LOCUS       DAYYGS010000001       102073 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000001 DAYYGS010000000
VERSION     DAYYGS010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 102073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.001"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..2153)
                     /locus_tag="ACHA44_000001"
     CDS             complement(<1..2153)
                     /locus_tag="ACHA44_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903331.1"
                     /translation="MSQWNTIDELRDIFIRKYTDSELSNELEKACSEEYELMRDYNGR
                     QILELLQNVDDAYGDIKSENDASNDEVKVQITYKNNILEVGNTGTSFSKDTIERLCLG
                     RASNKSSQNIGNKGTGFRSLLNDAEWVELYSGEFAIRFSEEFTKKLFEQYVSRDSDKF
                     SELIFEQKKNWKKEDYDLCFPIMNCPQPINKIDSGFDTLIRVKLKETNLSKGTSVSKQ
                     LQQPFYKSLLFLPNITKIVIETTDDEKISEKIVDGNDVLIEKSDVTGQSPSEEYFVFD
                     KESMIGDKVAKIALAVSKDSDYDYSKEKLYCYFPVRNFGTPIHALINAPFITNNSRDD
                     VPDDSEQINKKIFIEVLKFISEVAEKLSKPEYEDIALKMVTPFKENKLWDLDVFDLMN
                     EFLGILSNAQILPTVNWQYISIKDNPKLFLQEFPEELIGETFSTLLQLLETNENDQLV
                     QELANYNEYTGLSFVVDELSDKINILSSSWENRIRTKVFLWWSEQYKSSPIVPKLLKD
                     NSDNWLMKSDKVFLPTDTGVTVLPKELSSWVSLSILKQTYVDELIYQIKTSSSWKNKW
                     EETANAYKAERTGDKRILDAFSEKYLAIEFTEQSSSDLIIGTINRQIDTVEKSISFIN
                     WFYDNYNEKLSAGSELSKVPFNLPNVAGDVQPSNKMYIGKEYGNELGDKLFQDTDYTA
                     LKKIDCSSFAEERDFLSFILRCGVSKYPKIYDNDS"
     gene            complement(2276..3826)
                     /locus_tag="ACHA44_000002"
     CDS             complement(2276..3826)
                     /locus_tag="ACHA44_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8903332.1"
                     /translation="MAGKQVTVIPMKPRQWAAENTEEKPKLKVAAYCRVSTEMEEQAS
                     SYEAQVQHYTDYIQRNPDWELVGIFADEGISGTGTKKRDGFNRMIEACQNGEVEYIIT
                     KSISRFARNTVDCLQYIRQLKELKIAVFFEKENINTMDAKGEVLLTIMASLAQQESQS
                     LSQNTKMGVQYRFQQGQLRINHNHFLGYTKDEDGNLVIEPKEAEVIKRIFREYLEGSS
                     LQEIANGLMADGILTGGKRKLWRGEGVRLILRNEKYMGDALLQKTYTTDFLTKKRVKN
                     DGSYAQQYYVEDSHPAIIPKDIFMQAQQELDRRKSMKNKKSQCFSGKYALSGITVCGD
                     CGNAYRRVHWKNRGTVWRCKSRVDKREHNCTGRTIYETDLHGAVIKAINETLIDRDAF
                     LQQLNDNINSVMTDGLTDRLAELDSQLKELEAEIISLAVGGQGYDELASQIFSLRDER
                     DAVAKQIAANTNLQQRVDEMVVFVKEHDVINEYNEVLVRRLIEKVTIFEKNIVVDFKS
                     GVRVTVEI"
     gene            complement(3828..4193)
                     /locus_tag="ACHA44_000003"
     CDS             complement(3828..4193)
                     /locus_tag="ACHA44_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8903333.1"
                     /translation="MDEQQAQQIREFFNIYLEGVGLQEAGKRSGINKPHPFMKRILMN
                     EVYLGNEIYPKIIDEDIFFQADLEQERRIKFFKKRKEIKVPDTKVIPTHFKSGKITEK
                     YSDPFKQAQYAYSKIKEVK"
     gene            complement(4241..5794)
                     /locus_tag="ACHA44_000004"
     CDS             complement(4241..5794)
                     /locus_tag="ACHA44_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8903334.1"
                     /translation="MKTIHKLARPELPEASKLKVAAYARVSTSSNEQLASLQTQITHY
                     ENHIKNHEQWDYVGVYYDEGISGTKTVKRDGLKRLIKDAELGKIDLILTKSISRFSRN
                     TVDCLNLVRQLTDIGVTIFFEKENINTGDMESELLLSILSSLAESESYSHSENLKWAI
                     RNRMSKGKFKAYPPYGYKRKGYDFHIVAAEAKVVKQMFQWALDGISTGMIAKRLNDGK
                     QFTKRGANWQATSVRSILKNEVYTGTLTMQQTFVDDQYKRRINKGELPMYQIKNNHEP
                     IVSKEDYDRVQELIRRKAEAKGNTGDGKKYTKRYAFSGNIICDKCGSKFKRVHTYWKG
                     NTKKVQWKCTGHLKNKDSCDALPITDEDLKVAYLTMLNKLIFGHKLVLEPFMKSLNKA
                     GANQEGLEVLADEIDKIQEKLEVLASLTASSILSTKVSLEEQSKLQQERAVAQEKQRL
                     IIESVNGKSTQQIELEALYKFARMAEMLTDWDEDLFKKFTNSITVYNRQEVGFELKCG
                     LLLKERLPE"
     gene            complement(5855..6028)
                     /locus_tag="ACHA44_000005"
     CDS             complement(5855..6028)
                     /locus_tag="ACHA44_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8903335.1"
                     /translation="MTNKKTEINYSDEAAYYISQQILLNLLVTGKINLEEYVKIDNKN
                     RETFNPFLSRIMV"
     gene            6323..6628
                     /locus_tag="ACHA44_000006"
     CDS             6323..6628
                     /locus_tag="ACHA44_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019299341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903336.1"
                     /translation="MSSFDMEGLEKEYEHFCKSATTKELEEFDESFKQEFEKCFKTIE
                     AEEALYQFLQANINAMNNRMESIESPLFCINYKEQVFQLRNHLILMQNAVNTYLKEN"
     gene            complement(6689..7726)
                     /locus_tag="ACHA44_000007"
     CDS             complement(6689..7726)
                     /locus_tag="ACHA44_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014572406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8903337.1"
                     /translation="MVTFKDLLPEPTESQQAFILDHGKTEDDGQLKYADDAKSYGWNM
                     RQYGKLKAGAVVLNRHPGKITKDRKWEIYGGGYVESISEPDEAGNVTAVITHAFTIEP
                     AIKQGDSFIENFDWNTPSKKKRKKPNSWAYFWDQYGMNEITYADFVALIENRHLIPID
                     DTQSLPSELDLTNEEVEEIEQESSKGFTVLVDEVGPNRTNGTQKRKFTGRHTDWERVN
                     KAKQKTGALGEEIVLDFLIQEAEKNKTKLPEHVSKTEGDGHGYDICAFDQSGNEIHIE
                     VKASKTNFSDGFEMSTNEVAASLGDTPYKIYFVHDLDVASKVCKIKIYDGPITEENFT
                     MVPTSYKIFNK"
     gene            complement(7842..8750)
                     /locus_tag="ACHA44_000008"
     CDS             complement(7842..8750)
                     /locus_tag="ACHA44_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8903338.1"
                     /translation="MGISNLATKYGFQSFSHYSNGRSGAKVDKIVIHHMATTNYDIVP
                     DIWKTREASAHYGIGKNGEIRAYVDEGNTAWHAGNWNANISSIGIEHCNSTGSPNWGI
                     NQATIDASAKLCADIAKRYGLGKLVVNKNIFPHSHFSATSCPGQLLGKLQEIADKANA
                     INNGSSTPSTPASGLYRVRKSWADAKSQIGAYKDLNNAKRAADSHSGYKVFDQNGNQV
                     YPSGSSTSNVKTLVVQVSGLNVRNKPSLSGAVVGSYNKGATWTLPKSEKMTIADGWVW
                     ARCPIGYCAVGKNTGKTESDDYILIS"
     gene            complement(8740..9159)
                     /locus_tag="ACHA44_000009"
     CDS             complement(8740..9159)
                     /locus_tag="ACHA44_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin family protein"
                     /protein_id="HFM8903339.1"
                     /translation="MKEVWNWIQLAIAGGGGVIGWYVGGIDGLIYTLVTFVVVDYVTG
                     LLRAINEKKLSSKIGAKGITKKIMIFLLVGVAHMLDVEVIKGGNVLRDAVIFFYISNE
                     GISLLENAVAIGLPVPEKLREILQQLHEKKEETENGN"
     gene            complement(9160..9567)
                     /locus_tag="ACHA44_000010"
     CDS             complement(9160..9567)
                     /locus_tag="ACHA44_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HFM8903340.1"
                     /translation="MLKIRNEDLVDVMSFLKGFDLPPKISRVRTKLAKLIQVKVDELY
                     QDEVDLLQKYGKKDEHGNLVQHDGAFSLIPETATEYHKEKADLLNEETHINVDELQDK
                     LPLLVAGFENSEVTISGKDAEMLDLILEQLEDL"
     gene            complement(9554..9721)
                     /locus_tag="ACHA44_000011"
     CDS             complement(9554..9721)
                     /locus_tag="ACHA44_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903341.1"
                     /translation="MNEEKEEVIVTEPIEADIPKPMKVGTDMVDLLVMVVDDLCVQIG
                     DLKEKIDNAQN"
     gene            complement(9731..13621)
                     /locus_tag="ACHA44_000012"
     CDS             complement(9731..13621)
                     /locus_tag="ACHA44_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8903342.1"
                     /translation="MITLYNKTESNFNHNGLAVLDGNILNPVVSEELNGLFSFEFDYP
                     IHAPHSEELLPEMIVKAPVPELSDQLFRIVERDDALGGFLHIVAHHIFYDLATNFIED
                     TYIVNKNGSGALTQLLGATQFMHRFTGTSNISAVNNVRLVRLNPVEVLLDSDLENGFQ
                     SSYGGEIVRDNFTISMLTTRGSNNGVQIRDKKNLTGYKADVDYSGIVTRIMPQGYDGL
                     FLPEKYVDSPNINQYVQPKIKVIKYDNVKVGDEEDEFTQAEAYDELRRLAGLEYSANK
                     VDLPKATYDVEFAPLERTEEYKDFAQLETINLGDTVSVIHAEDGLNITARMVSYKYDP
                     FLKAYISVTLGNITPKFTDVAKDINRVDNKAEQAKDDANYALSSANGKNTNFYGPTTP
                     NNPKLGDVWYKENGDKFEMWVYEIRDGVTQWYPLMTDMTAEEVKQAVEQAQKDANTAT
                     EKANQAFNDAVNALNAAESVGASVETLTTSVEQNTSKISLVASGMSTLEGRVITAEST
                     LTVQAGQIASKASQSTVDNLTGRITTAESTITQNADRFELSLSKTNKAVNVLAGMSIA
                     DLQWAVGGLNTNDGKENTYGGYVRSGFTRVRNGEKYLLQTFVGGKLYSTYATAYLLYY
                     KEDKSFLSYTQFGNTTTPLTVPSSAAFIRIRLTTSDAPETISCYMLKTDVTNGYVDLS
                     EISNVVKLTATTDALLLSVSETKEAIGVPFKVNQWEQGSLSTTDGSEVSATNYLRSEF
                     LDVKVGDKYIGQLRDGTQAAFYYHYYGMDLPYIDFVPTAKQFSETLTAGTYTNATIAS
                     YLVSKNVESMSITSGVTTNPYSNSGDYLVIYKLPLEGKVAPSVISWNGRKDTSDNIVL
                     LYTGGAWEQISTVTSSSNATYSWTLTAEQISGITTDSVYLAFFSVKTGTYAGIYNSSN
                     TPFTLNPSDGSSYHQISYTGSSGAITVPSGTLKMRVRVNTSIKPDEYEGNIYPTTTRQ
                     DYTNANTLYSAILMQKDLINLRVGKGDVINQINISPESILIAGNKVHITGQTTIDSAV
                     ISSAMIQNAAITNAKIADATIQSAKIASIDAAKITTGTLSAARIGANSITADKIAANI
                     LTALTASSSIRITGTTIGYYSGSTLVTEINSQGMTIRRGGTTVGTIGANNISGHSDWR
                     GLVFDLEYGTEYMSWAHKDSSSASVYTQKLSWYARTLQSGMEKGFHFDDDIYLPSMLR
                     IGTQHQISVGGITFSSSLYTKIGSSSGSAGIAFNGSNLMLGDDGKWVDFGVIREICAK
                     LAGRTIALPTGFSSNGTASGWYNPQSFNSMTTYS"
     gene            complement(13618..14310)
                     /locus_tag="ACHA44_000013"
     CDS             complement(13618..14310)
                     /locus_tag="ACHA44_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8903343.1"
                     /translation="MNAFILDGTINSRTDLGLRITEVPVIPVTERVIDSIEIDGREGD
                     LTILKGWKDVTFGFKVVIWKSDVWTSWQIILPQILNAKTIAFSNEPNVFYPIKYAKAS
                     GLTQILSSMWEFEMELTCSPFRYKTNVATINRTTSGTVNNPGNIYSLPKIKVYGNGTR
                     TLTINGKPIVLNLTNGNLTLDSDLKECFYGDVAANQYMTGDFPEFRVGSNTVTLGTGI
                     TKVEIEPRWRYL"
     gene            complement(14387..14788)
                     /locus_tag="ACHA44_000014"
     CDS             complement(14387..14788)
                     /locus_tag="ACHA44_000014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903344.1"
                     /translation="MLKHKFKAKKPALIGYILLLAIGLFLTAVRWYSGINHGFVFINN
                     QINSHISNFALSFLFYLVIGYLWLLMGTNLRVLTAMGAGLIVANIVCETMMTFLNTPD
                     LIDAIYGISGVLVVYAYLAITKKIGLEETTE"
     gene            complement(14859..17420)
                     /locus_tag="ACHA44_000015"
     CDS             complement(14859..17420)
                     /locus_tag="ACHA44_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8903345.1"
                     /translation="MADNFGLKIGVEGEKEFKNSLREINQSFKVLGSEMKLVSSEFDK
                     NDKSISSLSARNQVLNKSIDAQKEKITTLENALKNASDSFGENDKRTQNWTVQLNNAK
                     AQLNNMEREVANNEKAIDAMGTEEVDAAKKTDELGNELKDTGDEAEKSGSKFEKFGGV
                     LKGIGVAMGTVALAAGAAAVELGKQVVSAYADYEQLVGGVDTLFKDASSTVQKNADNA
                     FKTAGMSANEYMETVTGFSAALISSLGGDTKKAAGVADQAVTDMSDNANKMGTDIESI
                     QNAYQGFAKQNYGMLDNLKLGFGGTKSEMERLLQEAEKISGVKYDISNFADVTEAIHV
                     IQTEMGITGTTAKEASETISGSIDGMKSAISNLMAGLGNSSADIGMLIGNVVEQFQNV
                     IKNILPVIQNIVSALPAVVDAIIQALGQLLPTLLEAVTSLFSQVLETILGLLPTLIPV
                     VVQAVLTIVQTLIENLPLLIDAAFQLVTTLIQGLGEALPELIPITVQAIITIVNGLIE
                     NLPLLLDAALQLIMGLAQGLITALPILIESLPQIITGIVNFIISAIPQIIQTGIQLLT
                     SLVTALPDIINAIVAAIPVIIQNIINAVIGAIPQLIDAGIQLLVALIGALPQIITTIA
                     NAMPQIINGITGTLVNNIDKIINAGVKLLVALVQNLPAIISAIVKAVPQIITGLANAF
                     GEYVSTMGTIGKNLITGLWNGIADAGSWLRNKISGFFGGVVDNIKDFFGIHSPSTLFR
                     DEIGKYMAQGIGVGFDKEMTGVSKTMQKAIPTKLYMPSFDIDTDIHAAVDSTFSMLDL
                     GAKVDALTNITSEMLPTLLKAMDIKVVLNDGTLVGKLAPEIDKSLATLNRRRVAY"
     gene            complement(17627..18007)
                     /locus_tag="ACHA44_000016"
     CDS             complement(17627..18007)
                     /locus_tag="ACHA44_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903346.1"
                     /translation="MSEERSAKISLGGDEYELILTTKATKEIASHYGGLDNLGEKLLK
                     SENFELALDEIIWLITLLANQSIKIYNLKNTEKKDELTTEYVELMTSPLQLAEYKSAI
                     TEAMFKGTARNIESETDSKNKAGE"
     gene            complement(18017..18607)
                     /locus_tag="ACHA44_000017"
     CDS             complement(18017..18607)
                     /locus_tag="ACHA44_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8903347.1"
                     /translation="MATIGLDKLYYAPITESATTGEETYGTPAMLAKAISAELSVELA
                     EATLYADDGASEVVKEFKNGKLTLGVDDIGRAVAAVLVGATVDQNGVLISTTEDGGTP
                     VAIGFRARKSNGKYKYLWLYRVKFAVPSTSLATKGDDITFSTPTIEGTVMRRNKLDAS
                     GKHPWKAEVDENDTGVEQTTINGWYTSVYEPDFTAD"
     gene            complement(18610..18951)
                     /locus_tag="ACHA44_000018"
     CDS             complement(18610..18951)
                     /locus_tag="ACHA44_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009052882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903348.1"
                     /translation="MNILEELNSLFATLHIPVETGEFSDKAPDKYCVLTPLADRFDVY
                     GDNFPTVDVQEVRISLFVKGNYLNTQKQLVQNLLKHEFTITERLFVGFEKDTNYYHVA
                     IDVAKHYEMEV"
     gene            complement(18948..19379)
                     /locus_tag="ACHA44_000019"
     CDS             complement(18948..19379)
                     /locus_tag="ACHA44_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8903349.1"
                     /translation="MAKALMKMPEDFLLKVSKLENKTDEIIPKVLESGAEVVEGKVRS
                     NLTAVVGSNTKVPSRSTGELESALGITQARQDKSGNWNIKVGFDEPRIDGSSNAKIAN
                     ILEYGKHGQPAKPFLKPAKSQSRKSCIETMKSKMESEVNGI"
     gene            complement(19372..19710)
                     /locus_tag="ACHA44_000020"
     CDS             complement(19372..19710)
                     /locus_tag="ACHA44_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8903350.1"
                     /translation="MSFGKMNQRIQIVKVTNQKDSAGFSVKQDEVLAAVHAYKEEKNA
                     TEKWVNMATFSSATCLFRIRYIPNLEVTTEMVVISDQKRYQIVSVENVRNRNMYLEML
                     TTEMEVSADG"
     gene            complement(19707..20018)
                     /locus_tag="ACHA44_000021"
     CDS             complement(19707..20018)
                     /locus_tag="ACHA44_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8903351.1"
                     /translation="MSDLLNKVKANLILEHNEDDELLERLILTALSYAESYQHIESGY
                     YSEHEMPPTTEQAVIVLASNFYESRDGSTGGFFADNVNASKQVWETVNLLLRLDRRWQ
                     V"
     gene            complement(20029..21240)
                     /locus_tag="ACHA44_000022"
     CDS             complement(20029..21240)
                     /locus_tag="ACHA44_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8903352.1"
                     /translation="MNKIQELMEKRAQAWNGAKAFVEAKQDKDGLMSEEDAKTYAEME
                     TKVKNFTMEIERMQSMEAMDRELEKPMSQPLTSQPMTTENKMEPKKTGRASAEYKEGV
                     LEALRSNFRHVSNVLQEGVDTAGGYLVPEEYDSRLIDGMTSENIMRTLATTITTSGEH
                     KINIAGSKPAASWIEEGGELSFGDATFDQILLDAHKLHVAIKVTDELLYDNAFNLENY
                     IIDQFTKALANAEEDAFLNGDGVGKPLGIFAATGGGQVAITTNTQSAVTADDVVNLVY
                     ALKRPYRKNAKFIMNDQTISLLRKLKDNNGAYMWQPALVAGEPDRLLGYPVYTSEFVP
                     TVKAGAPVIAFGDFSYYNIGDRGVRSFDQLRELFAGNGMVGFLAKERVDGKLVLPEAV
                     QILKMKASASS"
     gene            complement(21244..21999)
                     /locus_tag="ACHA44_000023"
     CDS             complement(21244..21999)
                     /locus_tag="ACHA44_000023"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
                     /protein_id="HFM8903353.1"
                     /translation="MKKFWKWKNQTVLNQDNQEEILERTLYLNGPIAEESWFDDEVTP
                     QIFKDELNSGGGNVTVWINSGGGDCVAAASIYNMLADYKGKVTVKIDGIAASAASVIA
                     MAGDKVLMSPVAMVMIHNPSAVAFGEKVDMEKAIAMLDEVKASIINAYERKTNLSRVQ
                     LAHMMDAETWMDANKAVHLGFADEIIGEIDPDIPVNNSMYSEVKVTNSLTSAIAKKCR
                     IEPPEPKEREEINNTIPADSLMSRLNLMKNWRN"
     gene            complement(21899..23227)
                     /locus_tag="ACHA44_000024"
     CDS             complement(21899..23227)
                     /locus_tag="ACHA44_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8903354.1"
                     /translation="MGIIKSLFKSRDKPTNQTIMNPYRFMFGGSSAGKSVTEQTAMQM
                     TAVYSCVRVLSEAVAGLPLHFYQYRDAGGKDKAMKHSLYFLLHDEPNSEMTSFVFRET
                     LMTHLLLWGNAYAQIIRNGKGEVVALYPLMPDKMTVNRDDNKEIYYLYQADVGQVRLS
                     KADVLHIPGLGFDGLVGYSPIAMAKNAIGLAIATEEYGSKFFANGANPGGVLEHPGTL
                     KDPGKLRESWNATFGGSSNAAKVAVLEEGMKYTPISMSPEQAQFLETRKFQLEEIARI
                     FRVPLHMVGDLDHATFSNVEHLSLDFVKFSLSPWLNRWEQALNKSLLSRDEKEKYFIK
                     FNVDGLLRGDYATRQQGYATGIQNGFLSPDDVRELEDLNPIPGGAGAKYYFNGNMLPI
                     EQAGAAYGVNKEESEEQDEEVLEVEESNGSESRQPRGNPRENPLPQRTNR"
     gene            complement(23293..24027)
                     /locus_tag="ACHA44_000025"
     CDS             complement(23293..24027)
                     /locus_tag="ACHA44_000025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903355.1"
                     /translation="MNEYLIQVAPDKISSDPDNTLISRLFYRKWWGKILTEDLTKYAE
                     GIGNYRGETIISFNTIAGATIRLLKCYNQKNFSMPDSHYGRLKVILYSDDITEEMKVK
                     FVKFYDLSHTLANFMPLYVSKPSLNGVKGKGPYHDFPDLFYSDVRDKRYGSSTNADKF
                     IVNNKDYFSKFKDWKSFIELNYLQDFFDDEDYMTFKKLAPSSSFFPYQRKSLTPDEKS
                     KCLEEVNTFLNNAIEIILSRGSRFYI"
     gene            complement(24083..25648)
                     /locus_tag="ACHA44_000026"
     CDS             complement(24083..25648)
                     /locus_tag="ACHA44_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8903356.1"
                     /translation="MDKDSHYDKSRADYAVAFIELLKHTKGQWFGKPFKLMPWQEKII
                     RDLFGTIKANGYRQFNTAYVEIPKKNGKSELAAAVALLLTCGDGEERAEVYGCAADRA
                     QASIVFDVAVDMIRQNPALSKRVKILTSQKRIIYQPTNSFYQVLSAEAYSKHGLNSSA
                     VVFDELHAQPNRKLFDVMTKGAGDARTQPLFFLITTAGNDTNSICYEQHQKALDIIEG
                     RKHDKTFYPLIYGIKQDDDWTDPEVWKKANPSLGITIAEEKVVAACDSAKQNPAEENS
                     FRQLRLNQWVKQAVRWMPMDKWDACSFNADEESLRGRVCYGGLDLSSTTDLTSFVLVF
                     PPEDEDDKFVILPYYWVPEDTLDLRVKRDHVNYDLWAKQGYIQTTEGNVVHYGFIEKF
                     IEELGEKYNIREIAFDRWGAVQMVQNLEGMGFTVVPFGQGFKDMSPATKELMKLTLEQ
                     KLAHGGHPVLRWNMDNIFVRTDPAGNIKADKEKSTEKIDGAIATIMALDRAIRCGNDN
                     GASVYDDRGLLFL"
     gene            complement(25731..25874)
                     /locus_tag="ACHA44_000027"
     CDS             complement(25731..25874)
                     /locus_tag="ACHA44_000027"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903357.1"
                     /translation="MMDYQIYMPDNEDGIEEGYYDWQELVQLLRDNKNNPEAIQFIAD
                     MME"
     gene            complement(25871..26047)
                     /locus_tag="ACHA44_000028"
     CDS             complement(25871..26047)
                     /locus_tag="ACHA44_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5049 domain-containing protein"
                     /protein_id="HFM8903358.1"
                     /translation="MNAKIKQQIIEIAESGLTNMFDAIAVQRIAHDRHFYELVIFIEE
                     HKDEYSRFILEGDK"
     gene            complement(26031..26225)
                     /locus_tag="ACHA44_000029"
     CDS             complement(26031..26225)
                     /locus_tag="ACHA44_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903359.1"
                     /translation="MKVSKRKIYNIAKNYIVQLPERGDLKAHNSDREDFLDIAVWCLE
                     DALIAAYEAGRKDGQNERKD"
     gene            complement(26227..26604)
                     /locus_tag="ACHA44_000030"
     CDS             complement(26227..26604)
                     /locus_tag="ACHA44_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4314 domain-containing protein"
                     /protein_id="HFM8903360.1"
                     /translation="MFPTHEQIEGMKKRYPEGSRVELIKMNDPHAPAKGTQGTVTGVD
                     DIGSILIQWDNGSTLNAVYGEDVVHIIKPDFELVFQNGRREGFETFNDAWQYVTDMIL
                     NHDLVWVDLESKTEDTIYIRKGL"
     gene            complement(26678..27913)
                     /locus_tag="ACHA44_000031"
     CDS             complement(26678..27913)
                     /locus_tag="ACHA44_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFM8903361.1"
                     /translation="MNTTERFEKVDIDKLVPYARNARTHSKDQVKQIQASMREFGFIN
                     PVIVDKDYNLIAGHGRILAAKEEGIKQVPCVFVEHLTEAQKRAYILADNRLAMNAGWD
                     DEMLAVELSDLQGEEFDLDLLGFETDELDKLLNGNDDAQDDDFDVDEELEKPAFSKTG
                     DLWLLGRHRLVCGDSTKPETYEKLMDGKLANLVVTDPPYGVSYQGKAGTIKNDNLKDE
                     AFYNFLYDAFSCMESVMANDASIYVFHADTEGLNFRKAFVDAGFYLSGTCIWKKQSLV
                     LGRSPYQWQHEPVLYGWKKKGKHQWYTGRKESTIWEFDKPKKNGDHPTMKPIPLLAYP
                     ITNSSLSNCIVLDSFGGSGSTLIACEQTDRICHTIELDEKFCDVIVKRYIEQAGSDAD
                     VSVIRDGKTIAFNDLENPE"
     gene            complement(27913..28695)
                     /locus_tag="ACHA44_000032"
     CDS             complement(27913..28695)
                     /locus_tag="ACHA44_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HFM8903362.1"
                     /translation="MIEKVNPQHPDKVADRIAGAIVDLAYAEENNPKIAVEVLIGHGI
                     CHVIIETSTALNLNEISQAINRIAGKIKKDVVIVPQDKELADNQATEIRCGDNGIFRG
                     MPLTDEQLQLSKIARDLYQDHPFDGKYILDGERLIICQSHAKTNELKQKYPNATVNPL
                     GDWTGGPDVDTGATNRKLGSDMADGVTGGGIHGKDLSKADVSVNIYAFLKAQETGMPV
                     ELSCAIGDTEIDGVPYSEIVEVARDYVNELGGFEKLAEWGLF"
     gene            complement(28710..29282)
                     /locus_tag="ACHA44_000033"
     CDS             complement(28710..29282)
                     /locus_tag="ACHA44_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006702252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8903363.1"
                     /translation="MAKDGTQRGGRRPRSGERSQPLVDKVAAGKKASRMEFDLPDVEL
                     LIGEDIGEGVELEGNDYPEPSAYLSAKQKDGTVLGADEIYNSTFQWLKDRGCETLIAP
                     RMVESYAQNFARFVQCENALSQYGLLGKHPTTKAVMASPFVAMSQNFQKQANVTWYEI
                     QQIVKENASVEYTGDPNTDAMERLLRSKNG"
     gene            complement(29416..29781)
                     /locus_tag="ACHA44_000034"
     CDS             complement(29416..29781)
                     /locus_tag="ACHA44_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002362768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8903364.1"
                     /translation="MPMKPANPCKFPGCPNRTHDTYCDEHKTLARKRYEKYERDPAIN
                     KRYGRAWKKIRARYVAAHPLCEMCLKEGRFTSVEIVHHIKELSDGGTHDENNLMSVCK
                     SCHSKIHMTRRNTKDKTIM"
     gene            complement(29949..30377)
                     /locus_tag="ACHA44_000035"
     CDS             complement(29949..30377)
                     /locus_tag="ACHA44_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HFM8903365.1"
                     /translation="MTPKEYLKQAYRLNKRIDDRIKEKAELYQLAVTVSSTTFEEKIS
                     SGTRNTEAPFVRNLEKCYQLEAEIDAEIDKFVDLKRQMRGVIDELTNVDEKIVLINRY
                     LLEESWDKIAEDLHADVRTVFRWHSKALENLKLPDEPIVI"
     gene            complement(30374..31726)
                     /locus_tag="ACHA44_000036"
     CDS             complement(30374..31726)
                     /locus_tag="ACHA44_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFM8903366.1"
                     /translation="MKFVPHNYQKYAIDFIKDHKTAALLLDMGLGKTVTTLTAIKDLM
                     HDDFTIQRVLIIAPLRVTQSTWPTEIQKWDHLKDLSYSVVLGTPSQRMAALQNNANIY
                     LINRENLDWLINKSGFTFDFDMVVIDELSSFKNLKAKRFTSFMKVRHKVDRIVGLTGT
                     PSSNGLMDLFAEFKVLDMGERLGYYISRYRDKYFLPDKQNGMQIYSWKPREDAEQEIY
                     DKISDITISMKSVDFLDMPELVMNEVPVDMGLKEKQKYDEIKADLVLQLKEATIDAAN
                     TAVLSNKLLQMANGAIYDEFNVSHHIHDQKLDALEDLIEGTNGKPILIAYWFQHDLER
                     IKERFNVRQIKTSQDIIDWNEGSIPIAVIHPASAGHGLNLQAGGSTLVWFGLTWSLEL
                     YQQTNARLWRQGQSDTVVIHHIIAKDTIDEDVMLALKRKDKTQSCLIDAVKARLEGSK
                     "
     gene            complement(31726..32010)
                     /locus_tag="ACHA44_000037"
     CDS             complement(31726..32010)
                     /locus_tag="ACHA44_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFM8903367.1"
                     /translation="MREKQVEQALVKAVKRVGGICPKFTSPGLAGVPDRLVLMPNGKL
                     GFVEVKAPGKKPRSLQLFRMKQLTDLGFQCFVLDEIDQIPELLERIGGDV"
     gene            complement(32007..32216)
                     /locus_tag="ACHA44_000038"
     CDS             complement(32007..32216)
                     /locus_tag="ACHA44_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903368.1"
                     /translation="MKFKQWLQIGIGKRDLRFDEFYEFAMSDVTYPWKKSFDKQVAHL
                     MIHCAEKEYLEILRDAYFEYLTVWL"
     gene            complement(32354..34597)
                     /locus_tag="ACHA44_000039"
     CDS             complement(32354..34597)
                     /locus_tag="ACHA44_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HFM8903369.1"
                     /translation="MLKMTLFTSNQVGNSKNGLFPTKQVVTDKETLVATVKLDHVMAE
                     YKGNYRKGDNFIQSDVIPLDCDNDHSDNPDDWVAPFEVAMQFPDVTFAVSYSRNNMKD
                     KGGKSARPRFHVYFPIPATTDKDEYVELKKQIQSEFPYFDNNAMDAARLLFGTPNSEV
                     EIYEGSKLITDFLEDDLFADWDNAQSEISEGSRNSSMSHIAGKLIKRYGATEDTYHKY
                     LEQSQKCNPPLPEEELKAIWNSAVNFGKKVKQQEGYIPPEQYNSDLSLEPTDYSDLGQ
                     ATVLAREYECKLHYSPSTGYLVYNGSYWEESKPKAQGVIHALTERQLEESETEIEKRT
                     KEMVSNGAFDVLASVGPKKAVTMFNTAQRHSFDLYQHAQEYKKFAVKRRDSKYLSSAL
                     TEAKPMLEIEQRLLDVNEFLLNTPTATFDLRTGKSQDHNSEDYITKQTECAPSDANQQ
                     IWLDALNTIFVGDQELIDYVQMIVGLAAIGKVYVEALIISYGEGRNGKSTFWNVISRV
                     LGNYSGSISADILTSQIRRNVKPELAEAKGKRLLIAAELEEGMRLNTSNIKQLCSTDE
                     IAAEKKYKDPFKYVPTHTLVLYTNHLPKVGAIDKGTWRRLIVIPFLATIDGNKDIKNY
                     ADYLFENAGGAILQWILDGAKKVIAADFHLTVPQVVANAISEYKTANDWLGHFLDECC
                     ETAVDFEEKSGELYAEYRAFCTRTGEYIRSSADFYTALEAGGFKRRRTNKGNKIVGLQ
                     LKSEFLE"
     gene            complement(34590..34934)
                     /locus_tag="ACHA44_000040"
     CDS             complement(34590..34934)
                     /locus_tag="ACHA44_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFM8903370.1"
                     /translation="MPRRKDYRTSVYVCSPFSGNVVVNHKNTKRYCRIALDRGYMPVA
                     AHLMYPQFMDDATERDVAMHMSLVLMGKCEEVWVVGDTISQGMAVEIEQAEYWKKHIR
                     YFDDEMKEVSNA"
     gene            complement(34927..35697)
                     /locus_tag="ACHA44_000041"
     CDS             complement(34927..35697)
                     /locus_tag="ACHA44_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014929981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HFM8903371.1"
                     /translation="MNELKVFENAEFGSVRTVMIGDVPHFVGKDVASILGYTNPQKAL
                     RDHVDEEDKTVNESFSVNGTMAILINESGLYSLIISSKMPNAKKFKRWVTNEVLPSIR
                     QHGVYATEDFITKSIEDPAWAISVLKQLQEKKEMVAMQQQMILEMKPKVSYYDLILQN
                     NSVMSISKISKDYGMSPQAMNKLLHELGIQYKQGKMWLLYQKYANQGYTQSKTHAIDA
                     ERSVMHTYWTQKGRLFLYDLLKSKKNILPVIERGDNNA"
     gene            complement(35908..37845)
                     /locus_tag="ACHA44_000042"
     CDS             complement(35908..37845)
                     /locus_tag="ACHA44_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFM8903372.1"
                     /translation="MKHLSLDLETYSSVDLSKCGVYKYSESPDFEILLFAYSVDGGPV
                     EVVDLKMDETIPAEILTSLSDETVIKHAFNASFERVCLSRFLGLDGFLNPRGWQCTMV
                     WGATLGLPLSLVGVGAVLGLDKQKLTEGKDLIRYFCVPCNPTKVNGGRTRNLPHHAPT
                     KWSAFKTYNSRDVEVEIAIQGKLSNFPVLDSVWKEYWLDQEINDRGIEIDKDFVEAAV
                     QLDELSKAGLNQQLQELTGLENPNSVLQMRGWLLDHGLETESLGKKAVAELLEQPIDE
                     NLKQVLVLRQQLAKSSVKKYQAMQNFACSDNRSRGLFQFYGANRTGRFAGRGIQLQNL
                     PQNHMSDLSEARSLVKQSNFEMLNILYGNVPNVLSELLRTAFVPREGMKFIVSDFSAI
                     EARVIAWLSGEKWRQEVFKNGGDIYCASASQMFGVPVEKHGVNGHLRQKGKIAELALG
                     YGGSVGALKAMDALEMGLTEDELQPLVNSWRDSNINITELWWAVDKAVKTCVKERRST
                     ATHGIKFEYKSGFLFIQLLSGRKLVYVKPKIGENKFGGEAVTYEGVGATKKWERLESY
                     GPKFVENIVQAIARDILCFAMENLRKYRIVGHVHDEVIIEVPMESKVEDICQLMSQTP
                     KWAEGLLLNADGYECEFYQKD"
     gene            complement(37906..38448)
                     /locus_tag="ACHA44_000043"
     CDS             complement(37906..38448)
                     /locus_tag="ACHA44_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFM8903373.1"
                     /translation="MSKTSKVITGTNTRLSYFHGWEPASINGGAEKYSTAVLIPKSDK
                     RTIDTIEKAIDAAIEEGLAKFGGKKPNKAEIKLPLRDGDIERDDEAYQGHYFINANST
                     TAPQIVDQHVQPILDRSQVYSGCYARVSINFYAFNANGNRGVAAGLGNIQFVRDGEPL
                     GSQVSASDEFETLADDDFLA"
     gene            complement(38450..39592)
                     /locus_tag="ACHA44_000044"
     CDS             complement(38450..39592)
                     /locus_tag="ACHA44_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFM8903374.1"
                     /translation="MPTQHAVLSASSSNRWIHCPPSVRLSEHFENKSSPYAQQGTDAH
                     TLCEYKLNKFIGNDVTDPTSTLEFYDEEMEQCAESYATFVMEEVAKAKQTTVDSIVIV
                     EQRLDFSRYVPDGFGTGDCLVIADGTLSVIDMKYGLGILVDAYQNPQMMCYALGALEL
                     FDVIYDIQEVKMTIFQPRRENVSTYTLLKEELLQWAKDVLSPAADLAFKGEGEYQCGK
                     WCGFCPAKNSCRARADHNLKLAQYEFKPPELLSDDEIEEIIGKVDDLVSWSNDIKDCA
                     LKLALGGKQWTHHKLVEGRSTRKYSNDNDVAAAVIKAGYDPYDKKLLGVTAMTKALGK
                     EKFNEILGEYIIKPKGKLTLVDGSDKRQAVTVNNLNEEFKPLTEEQ"
     gene            complement(39593..39907)
                     /locus_tag="ACHA44_000045"
     CDS             complement(39593..39907)
                     /locus_tag="ACHA44_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA biogenesis protein rrp5"
                     /protein_id="HFM8903375.1"
                     /translation="MSKTKLFLQVVEDVQALADSLQELAVAMKTNEPEPQVETPEPTM
                     ETKSEVKQPTLEEVRGLLARKSQEGKSTEVKALIEKYGASRLSDVPTENYAALVADAE
                     GL"
     gene            complement(39912..40118)
                     /locus_tag="ACHA44_000046"
     CDS             complement(39912..40118)
                     /locus_tag="ACHA44_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903376.1"
                     /translation="MNLKHKIRVNVTDDTGNKETVLQGGVRHLPRWFARWLFGKGNMD
                     KILVLTPGDTIESVEIREVNKKGA"
     gene            complement(40215..40832)
                     /locus_tag="ACHA44_000047"
     CDS             complement(40215..40832)
                     /locus_tag="ACHA44_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903377.1"
                     /translation="MSRFMKTHQNDRSTYKYVFERTDANGRIQKEVVEIRPGEDGVTE
                     LNIQELHRADDRAIYNFYKNARPERTEEEKEKIKAWKEKFREDFNNEHGYYPMDEYVD
                     GEANDYFKRNYNLSLNEFDDECDEGDNPIQSLVYDAYQDNEEDPRIDRLREVVTQLLP
                     YHQEIYHMLVTENMSQTEVADKLDKKKQSINRTFGIIKDKIKELF"
     gene            41227..42177
                     /locus_tag="ACHA44_000048"
     CDS             41227..42177
                     /locus_tag="ACHA44_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system protein"
                     /protein_id="HFM8903378.1"
                     /translation="MEISEITQTLYPFMGSEYVKPQDFLIALLDSVSNDDEATIFGFS
                     DDNLRRFYNGTRPLTQEYAVRVAGKFNIDKCTTFIYERLIDDAPTVLKQQLIDKGVVI
                     PKDTDVSEIVAHLLLDEITSIASQTKVKTKKAKPKLSELELASLRINTNGDFSYNDEV
                     KKFFDDLPVPLDIRAEEMTYVIALFSAYADADGYEVYEHTNDLPNSRLKDFKRQRRNY
                     YKAESIRRGVRDNFTQEEGTEHFEALKEDMYDGIEEVYEEDYDDGVERLKAVLQQSSV
                     ITLNGSILSFIPGLLQNSAKKGICHMLVNDGKIKWVTEDE"
     gene            42170..42616
                     /locus_tag="ACHA44_000049"
     CDS             42170..42616
                     /locus_tag="ACHA44_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-three component system middle component 2"
                     /protein_id="HFM8903379.1"
                     /translation="MNSLFNTPFEISVRVMLLLSEFKEPVSADRILITDFITTYGRDF
                     EISDYNLNGDNSYRFSEFIARRELVKDAVKNLVLQHVIQPSQSKEGFKYALSEDGLKL
                     ISQFVSVYAEKYLQIADESVKYIQDKSDVELLRQINQRAKEFKGEQ"
     gene            42619..44451
                     /locus_tag="ACHA44_000050"
     CDS             42619..44451
                     /locus_tag="ACHA44_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8903380.1"
                     /translation="MQNYYIERVTIKGKDREDATVKFTNGLNIISGPSNTGKTSIVTA
                     IDFLYGAKKDAAPFSTDATGYSKVELIIQSTNGKYSLTREFDSNSILVSKITSSGEYV
                     DYEKYNARSGKNPISHFWLQLLGIDDDYQIIKNEDFKRQKLTWRTLAPSTLIPQDRVN
                     TSASVLFPGETTQRTAFLSSLLLLLYGSDFANSEENETKEHKKIRRNSVINYINSSLS
                     ELYENYEVAKSELPEAPSDDFQQIVDGMFAELETIEKQINDALASSKILIKEIYQKEQ
                     YLHETQLLLNRYKQLHYQYQSDIERLTLIVDGERLLNDHNEHDTSHSCPFCNGELSKP
                     VQTTYIDTARSELHSLLSQLSDLTEESHDTEVELQELMEQLDELKTRKKSLDAAINDE
                     LKPKAETLKDRINKIETYRDIKQQLISMEKMQNKLKSDLEKIENEPEDERILYKPKEK
                     FDAEFYKEMSELLDSMLRNMHYKPNEFRNAFFAKDIFDIKINGNLKWTENGHGYTAFI
                     NSVVVMAYRHLLYNHAKYTPSVFIIDSPLLGLDERKKQDGDDNMKEGLFKYFMDHQNE
                     GQLIIVENTTNLPALNYDDKQVNQIVFTEDKNNGRYGYLYDIYN"
     gene            44467..44670
                     /locus_tag="ACHA44_000051"
     CDS             44467..44670
                     /locus_tag="ACHA44_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903381.1"
                     /translation="MKFSYNPLWKLLIDRGLNKAQLRKMTDISSATLSKLNKGENVTT
                     DILLRICEALDCDLSDIVEIVRD"
     gene            complement(44721..45857)
                     /locus_tag="ACHA44_000052"
     CDS             complement(44721..45857)
                     /locus_tag="ACHA44_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8903382.1"
                     /translation="MANSLDSIFEDDAFDDLTKQVEKKKVVKQDPEVAKFQEIIDFVN
                     ENGREPEKTPEWSSERALWARLSGFRGKPERSEKVKKYDTLGLLEKEFEPDGEFLADS
                     VEIDDELNLDDILGSDDMIDDFSDLLDVSRYRRTVNAADKIGRRKHASHFEKYKSLFD
                     QVHADIASGRRKIIPFEQYDIHENRFYIQNGVMMYIVSISKDTFIADNGKTNARMHVV
                     YENGTENKNLLLQSLASSLYSTERHGRMVTEPVDEETLAESFGEEFTTGYIYVLKSLS
                     TNPDISKWNNLYKIGFTRNDVESRIANAENDVTYLNAPVKVMLSAEVQNVNAQLLERT
                     LHHVFQDKQVVFQDGNFKKATEWYMVPLNDIESKINEVIAGLQK"
     gene            complement(45847..47802)
                     /locus_tag="ACHA44_000053"
     CDS             complement(45847..47802)
                     /locus_tag="ACHA44_000053"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8903383.1"
                     /translation="MTSNLFEINFDKNQSKTTNDLGMREMQERVYEKRASQYLLVKSP
                     PASGKSRALMFVGLDKLHNQGIKKVVIAVPERSIGKSFKDTELSKFGFYWDWEVKPSD
                     NLTIGGGEKSKVQHFVKFMNSTDPSDNILIATHATLRFAFEELDDSLFDNTLLAIDEF
                     HHVSRDDKSVLGNALRSIMSNSNAHILAMTGSYFRGDSVQILEPADEDKFDKVTYTYY
                     EQLEGYKYLKSFAMGYSFYRGKYTDALDKVIDVNKKSIIHIPNVNSGESTKDKYAEVD
                     EILDLLTDGGNITQNEDGIYEIERPDGKKLLVADLVNEEGREKVSAYLASITDDIKDL
                     DKLDIIIALGMAKEGFDWPFAEYALTIGYRNSLTEVIQIIGRVTRDSPNKTHAQFTNL
                     IAQPDAQDDEVLFAVNSIMKAISASLLMEQVLAPVYKFKPKDGKPDKPGDLSIRGLKP
                     ADTTERTKKIIENDMEDLKSSILQSQDIQNAIVSGADAKMINKTLIPRVIIDRYPDLT
                     NEELETVRQHTVADINIAAGRTTTSEDGSREIIKMADRFINIDELNIDLIDQVNPFQR
                     AYEVISRDIDAPTLRFIQDYLSSNKYDFTDEQLVAAYQAAKKFRAENGRNPDRNSKDD
                     GEKYLAFALLRLAEIKREREAAKEDGE"
     gene            complement(47795..50512)
                     /locus_tag="ACHA44_000054"
     CDS             complement(47795..50512)
                     /locus_tag="ACHA44_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010821417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFM8903384.1"
                     /translation="MAKNKVLNITEIEDGVKKILSNRGDDFITQFLSLYDIPKTSITR
                     AKSKFDNGEPFVIKNKLHYEEIEGNVVIAIDAIAQSIKEQKSKPRYILVNDYLDIAAI
                     DTKTHETLNIPIEELPANADFFLAWNGIEKADYQAENPADRKAAERFAKLYDVVAKDN
                     PDANEHAFNLFLIRVLFLLFAEDTGIMNKGSFTNVLKTRTAEDGSNFNEVIKELFAIL
                     DINELARNGKDEWLLEFPYVNGKLFGEPHVDLTFSKVSRELLIEAGELLNWNEINPDI
                     LGAMIQSVASAEDRHVAGMHYTSVPNIMKVIKPLFLDDLTESFETLKLRSEENELKDI
                     TEKTRNDNKRTIINDLSALLTRISNIKFLDPASGSGNFLIIAYKEIRRLEIKILQLLG
                     DLQNNDSMPLSVIHLGNFNGIELDDFAHEVARLSLWIAEHQMNKELEEAIPGTIAELL
                     PLKDAGNIVCANSLRIDWNEVLRLKENHEIYIMGNPPYLGKSLQNAEQREDMEKLVGD
                     IRKYKLLDYIFGWFWKAKQYIATSHGSFAFVTTNSICQGEQVSILWPYLLKDEVRISF
                     AYQSFKWNNNAKNNAGVTVVIIGMTNNVNIHPKLYLENGSVMSVSNINAYLANGENVF
                     VNESRNSISNLPSLKFGSMANDGGNLVFSVAEYWNLIQKYPDIKHLFKKYLGSTGLIN
                     GDLRYTLWLTEEDATRYKQNSLIKETIKNVKSYREKSKRKETRALAEKPWSFGEVRYD
                     ETSENSILIPRVSSEKRDYVPMGIVGSDTIISDSAMAIYNAPMWLLGLLESRMHMVWL
                     RSVGGKLETRYRYSAGLVYNTFPIQDLSAQRKNEMTRVMTEILDLREYEGGTLADLYN
                     KDSMPESLRKKHQELDGIVDRAYQQRPFESDEDRLGTLLKLYQEMTIND"
     gene            complement(50513..50734)
                     /locus_tag="ACHA44_000055"
     CDS             complement(50513..50734)
                     /locus_tag="ACHA44_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903385.1"
                     /translation="MSISYNKLWKLLIDLNMNKTQLREISGVSPNVIAKLGKNELVAM
                     ESLLKIAAALNVDVSEIISSDVIDAEDVK"
     gene            complement(50883..51719)
                     /locus_tag="ACHA44_000056"
     CDS             complement(50883..51719)
                     /locus_tag="ACHA44_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8903386.1"
                     /translation="MKQIFIFSDTQKDNISYFEREPAILVSMSRSDLPYLKEQDFSNY
                     PAVYVLIGGNKRYVGQAAGQSISLRLSQHFLKEYKAWVESVLFFTRADGKMSKADTDY
                     LEKRLIQDFQEKSDYEMMNSTAGNTSYIDKLQKAKSDQLYDTVFEIIDEIANIDLFGT
                     SEDSETQIYEFSSSGQYEIEYDGKKISSKSARGLLVDFVSQLFADGNYNNQLQELIVD
                     ETPSFAIILGRKPSMYNGRPNSARVADGVWVYANFNKKNVRIKIDKLARQLGIPVKIK
                     WN"
     gene            complement(51928..53304)
                     /gene="rlmD"
                     /locus_tag="ACHA44_000057"
     CDS             complement(51928..53304)
                     /gene="rlmD"
                     /locus_tag="ACHA44_000057"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8903387.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIECV
                     GLLTRVER"
     gene            complement(53415..54470)
                     /locus_tag="ACHA44_000058"
     CDS             complement(53415..54470)
                     /locus_tag="ACHA44_000058"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8903388.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(54480..55910)
                     /gene="gatB"
                     /locus_tag="ACHA44_000059"
     CDS             complement(54480..55910)
                     /gene="gatB"
                     /locus_tag="ACHA44_000059"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFM8903389.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(55907..57379)
                     /gene="gatA"
                     /locus_tag="ACHA44_000060"
     CDS             complement(55907..57379)
                     /gene="gatA"
                     /locus_tag="ACHA44_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFM8903390.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(57379..57684)
                     /gene="gatC"
                     /locus_tag="ACHA44_000061"
     CDS             complement(57379..57684)
                     /gene="gatC"
                     /locus_tag="ACHA44_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFM8903391.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(57790..59826)
                     /gene="ligA"
                     /locus_tag="ACHA44_000062"
     CDS             complement(57790..59826)
                     /gene="ligA"
                     /locus_tag="ACHA44_000062"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFM8903392.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(59843..62077)
                     /gene="pcrA"
                     /locus_tag="ACHA44_000063"
     CDS             complement(59843..62077)
                     /gene="pcrA"
                     /locus_tag="ACHA44_000063"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HFM8903393.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            62232..63791
                     /locus_tag="ACHA44_000064"
     CDS             62232..63791
                     /locus_tag="ACHA44_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HFM8903394.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(63902..65833)
                     /locus_tag="ACHA44_000065"
     CDS             complement(63902..65833)
                     /locus_tag="ACHA44_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HFM8903395.1"
                     /translation="MNIKDLMVKEAMIMDLEATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(65851..66765)
                     /gene="pfkB"
                     /locus_tag="ACHA44_000066"
     CDS             complement(65851..66765)
                     /gene="pfkB"
                     /locus_tag="ACHA44_000066"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFM8903396.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(66762..67514)
                     /locus_tag="ACHA44_000067"
     CDS             complement(66762..67514)
                     /locus_tag="ACHA44_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8903397.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(67673..67882)
                     /locus_tag="ACHA44_000068"
     CDS             complement(67673..67882)
                     /locus_tag="ACHA44_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903398.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(67893..69221)
                     /locus_tag="ACHA44_000069"
     CDS             complement(67893..69221)
                     /locus_tag="ACHA44_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903399.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            69357..69512
                     /locus_tag="ACHA44_000070"
     CDS             69357..69512
                     /locus_tag="ACHA44_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HFM8903400.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(69586..70173)
                     /gene="yihA"
                     /locus_tag="ACHA44_000071"
     CDS             complement(69586..70173)
                     /gene="yihA"
                     /locus_tag="ACHA44_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFM8903401.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(70256..71506)
                     /gene="clpX"
                     /locus_tag="ACHA44_000072"
     CDS             complement(70256..71506)
                     /gene="clpX"
                     /locus_tag="ACHA44_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFM8903402.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(71753..73042)
                     /gene="tig"
                     /locus_tag="ACHA44_000073"
     CDS             complement(71753..73042)
                     /gene="tig"
                     /locus_tag="ACHA44_000073"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFM8903403.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(73196..74128)
                     /locus_tag="ACHA44_000074"
     CDS             complement(73196..74128)
                     /locus_tag="ACHA44_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903404.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(74285..74944)
                     /locus_tag="ACHA44_000075"
     CDS             complement(74285..74944)
                     /locus_tag="ACHA44_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFM8903405.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(74947..75972)
                     /gene="sppA"
                     /locus_tag="ACHA44_000076"
     CDS             complement(74947..75972)
                     /gene="sppA"
                     /locus_tag="ACHA44_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFM8903406.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(75985..76275)
                     /locus_tag="ACHA44_000077"
     CDS             complement(75985..76275)
                     /locus_tag="ACHA44_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903407.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            76685..78490
                     /gene="glmS"
                     /locus_tag="ACHA44_000078"
     CDS             76685..78490
                     /gene="glmS"
                     /locus_tag="ACHA44_000078"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFM8903408.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(78562..79917)
                     /gene="glmM"
                     /locus_tag="ACHA44_000079"
     CDS             complement(78562..79917)
                     /gene="glmM"
                     /locus_tag="ACHA44_000079"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFM8903409.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICARYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(79941..81110)
                     /locus_tag="ACHA44_000080"
     CDS             complement(79941..81110)
                     /locus_tag="ACHA44_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HFM8903410.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(81107..81994)
                     /gene="cdaA"
                     /locus_tag="ACHA44_000081"
     CDS             complement(81107..81994)
                     /gene="cdaA"
                     /locus_tag="ACHA44_000081"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HFM8903411.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(82183..85863)
                     /gene="nifJ"
                     /locus_tag="ACHA44_000082"
     CDS             complement(82183..85863)
                     /gene="nifJ"
                     /locus_tag="ACHA44_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFM8903412.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            86061..86582
                     /locus_tag="ACHA44_000083"
     CDS             86061..86582
                     /locus_tag="ACHA44_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HFM8903413.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(87281..87846)
                     /locus_tag="ACHA44_000084"
                     /pseudo
     CDS             complement(87281..87846)
                     /locus_tag="ACHA44_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(88025..88681)
                     /locus_tag="ACHA44_000085"
     CDS             complement(88025..88681)
                     /locus_tag="ACHA44_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903414.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(89249..90184)
                     /gene="whiA"
                     /locus_tag="ACHA44_000086"
     CDS             complement(89249..90184)
                     /gene="whiA"
                     /locus_tag="ACHA44_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HFM8903415.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(90218..91216)
                     /gene="yvcK"
                     /locus_tag="ACHA44_000087"
     CDS             complement(90218..91216)
                     /gene="yvcK"
                     /locus_tag="ACHA44_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFM8903416.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(91213..92097)
                     /gene="rapZ"
                     /locus_tag="ACHA44_000088"
     CDS             complement(91213..92097)
                     /gene="rapZ"
                     /locus_tag="ACHA44_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFM8903417.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(92232..92963)
                     /locus_tag="ACHA44_000089"
     CDS             complement(92232..92963)
                     /locus_tag="ACHA44_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HFM8903418.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(92967..94232)
                     /locus_tag="ACHA44_000090"
     CDS             complement(92967..94232)
                     /locus_tag="ACHA44_000090"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HFM8903419.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(94495..97311)
                     /gene="uvrA"
                     /locus_tag="ACHA44_000091"
     CDS             complement(94495..97311)
                     /gene="uvrA"
                     /locus_tag="ACHA44_000091"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFM8903420.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(97321..99315)
                     /gene="uvrB"
                     /locus_tag="ACHA44_000092"
     CDS             complement(97321..99315)
                     /gene="uvrB"
                     /locus_tag="ACHA44_000092"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFM8903421.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            99574..101076
                     /locus_tag="ACHA44_000093"
     CDS             99574..101076
                     /locus_tag="ACHA44_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HFM8903422.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            101078..101815
                     /locus_tag="ACHA44_000094"
     CDS             101078..101815
                     /locus_tag="ACHA44_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8903423.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
BASE COUNT        28237 a        22719 c        18132 g        32985 t
ORIGIN      
        1 aaagaatcgt tatcataaat cttaggatat ttggaaacac cacatcgaag aataaatgac
       61 agaaaatctc tctcctcagc aaaactagag caatctattt ttttcaatgc cgtgtaatct
      121 gtatcttgaa aaagcttatc tcctaattca ttgccatact cttttccaat atacatttta
      181 ttggaaggtt gaacgtcacc agcaacgttt ggaagattaa atggaacttt agacagctca
      241 cttccagctg atagtttttc attgtagtta tcataaaacc aattaatgaa actgatagat
      301 ttttcaacag tgtcaatttg tcgattaatc gttccaatga tcaaatctga actgctttgc
      361 tcagtgaatt caattgcaag atatttttca ctgaatgcat ctagaatacg tttatctcct
      421 gtacgttcag ctttgtatgc attagctgtt tcttcccatt tatttttcca acttgatgaa
      481 gtcttaatct gataaattaa ctcatcaaca tatgtttgtt tcaaaatgct taaactaacc
      541 caagaagaca gctcttttgg aagcacagta acacctgtat cggtcggtaa gaatacctta
      601 tctgatttca tcaaccaatt gtcactattg tcctttaata gcttagggac aatcggagaa
      661 cttttatatt gctcactcca ccataagaac acttttgtcc ttattctatt ttcccaagaa
      721 gatgaaagaa tgttgatttt atcagatagt tcatctacaa caaacgacag gccagtgtat
      781 tcattataat tagcaagttc ttgtactagt tggtcatttt cgtttgtttc taataattgc
      841 aacaaggtgg aaaatgtctc acctattagc tcctcaggga attcttgaag gaaaagtttt
      901 ggattatctt tgatagaaat atattgccaa ttgacagtcg gtagtatttg ggcattagat
      961 aaaataccca agaattcgtt cattaagtca aatacatcta agtcccatag tttattctct
     1021 ttaaaaggag ttaccatttt taatgctatg tcttcatatt caggcttaga taatttttct
     1081 gctacttcag aaataaattt gagcacctca ataaatattt tcttatttat ttgttcacta
     1141 tcatccggga cgtcatcacg tgaattattt gttataaacg gtgcatttat aagtgcatga
     1201 attggtgtac caaaattacg tactggaaag tagcaatata gtttttcttt ggagtaatcg
     1261 taatcagaat ctttagaaac ggctagtgca attttagcaa ctttatctcc tatcatagat
     1321 tctttgtcaa aaacaaaata ttcttcagac ggtgattgac cagtaacatc acttttttct
     1381 atcaatacat catttccatc cacaattttc tctgatattt tttcatcatc agttgtttca
     1441 ataacaattt ttgttatatt tggtaaaaat aacagtgact tataaaatgg ctgctgaagt
     1501 tgtttagaaa cactcgtgcc tttacttaaa ttagtttctt ttagttttac tcgaattaat
     1561 gtatcaaaac cagaatcaat tttgtttatt ggttgaggac agttcattat tggaaaacat
     1621 aaatcataat cttctttttt ccaatttttc ttctgctcaa aaattaattc actgaatttg
     1681 tcagaatctc ttgatacata ctgctcaaaa agcttttttg tgaattcttc actaaatcga
     1741 atagcaaatt ctcccgaata cagttcgacc cactcagcat cgtttagcaa ggaacgaaaa
     1801 ccagtcccct tatttccgat attttgtgat gatttattac tcgctcttcc taagcacaat
     1861 ctttcaattg tatcttttga aaatgaagtg cctgtattcc caacttccaa gatattattt
     1921 ttatatgtta tctgaacttt cacttcgtca ttgctagcat cattttctga ttttatatct
     1981 ccataggcat cgtctacatt ctgtaataac tctaaaatct gacgcccatt ataatcacgc
     2041 atcaattcat attcttcgct gcaggccttt tcaagttcat tactcaactc agaatccgta
     2101 tatttacgaa taaaaatatc ccttaattca tcaatggtat tccattggct cacagaactt
     2161 cgcccccagc ctatttttta cataatatat tatatcatca atcactttat gaaatccaat
     2221 caataaagac gttaatatgg ctacccacat taacgtctag tcctatctat tcaaattaaa
     2281 tttctacagt cactctcacc ccacttttga aatcaacaac aatgttcttt tcaaaaatgg
     2341 tgaccttctc aatcaacctt cgaacaagca cttcattata ctcattgatg acatcgtgtt
     2401 cttttacaaa gactaccatc tcatcaaccc gttgttgcaa attagtattc gctgctattt
     2461 gttttgcaac cgcatctcgt tcatctctta gtgaaaatat ttggcttgcc aattcatcgt
     2521 aaccctgacc gccaactgcc aggctaataa tttcagcttc aagctctttc aactgactgt
     2581 ctagttctgc caatcggtca gttaatccat cagtcataac tgagttgata ttgtcattta
     2641 attgctgaag aaaagcatca cgatcgatta acgtttcatt gattgccttg ataacagctc
     2701 catgcaggtc tgtttcgtag attgttcgac ccgtgcaatt atgttctcgt ttatccactc
     2761 tggatttgca tcgccacaca gttcctcgat tcttccaatg aactctgcgg taagcattgc
     2821 cacagtcacc gcaaactgtg ataccagata acgcatactt cccagaaaag cactgtgact
     2881 ttttgttttt cattgattta cgccggtcta gttcttgctg ggcttgcatg aagatatctt
     2941 ttgggataat ggccggatgg ctatcttcaa cataatactg ctgagcgtaa ctcccgtcat
     3001 tcttcactcg tttctttgtc aagaaatcgg tagtatatgt tttttgaagc agcgcatccc
     3061 ccatgtactt ctcgtttcga agaatcaatc tgactccttc tcctcgccag agctttcgct
     3121 tccctcccgt taggatgcca tcagccatta gaccattagc aatttcttgc aaggaactgc
     3181 cctcaaggta ttcgcggaag atgcgtttga tgacttctgc ttcctttggt tcaatcacca
     3241 agttaccatc ttcgtcctta gtgtacccaa gaaaatgatt gtggttgata cgtagctgtc
     3301 cctgctggaa acgatactgt acgcccatct tggtgttttg acttagtgac tggctttctt
     3361 gctgggccag acttgccata atggtcaaaa gtacctcacc cttcgcatcc attgtattga
     3421 tgttttcctt ttcaaaaaac actgcaatct tcagttcttt taactgtcgt atgtattgca
     3481 agcaatcaac cgtattacgg gcaaagcgtg aaattgattt tgtgatgatg tactcaactt
     3541 cgccgttttg acacgcctca atcatccggt taaaaccgtc acgctttttc gtcccagttc
     3601 ctgaaatccc ttcatccgca aatattccta caagctccca atcgggattt ctttgaatgt
     3661 agtccgtata gtgttgaacc tgtgcttcgt aagaactcgc ctgttcctcc atttcagtag
     3721 aaacacgaca ataggctgcc actttcaact ttggtttctc ttcggtattc tctgctgccc
     3781 attgtctagg cttcattgga ataaccgtca cctgttttcc tgccatttca cttaacctcc
     3841 ttgatttttg aatatgcata ttgcgcttgt ttgaatgggt cagaatactt ttcggtaatc
     3901 ttgccagatt taaaatgagt gggtattacc ttcgtatctg gcactttgat ctccttgcgt
     3961 tttttaaaga acttaattct tcgttcttgc tccagatccg cttggaagaa aatatcctca
     4021 tcaatgattt ttggatatat ctcatttcct aagtaaacct cattcatcaa aatacgtttc
     4081 ataaagggat gcggcttgtt aatgccagag cgtttgccag cttcttgtaa gccaacaccc
     4141 tcaaggtaaa tattgaagaa ctcacgtatt tgttgagctt gctgttcatc cacgttgatt
     4201 tctccattct taatgacata cccgtatgga attgtattgt tcattctggt aacctctctt
     4261 tcaaaagtag tccgcactta agttcaaatc cgacttcttg gcggttgtaa actgtaatgc
     4321 tgtttgtgaa cttcttgaat aaatcttcat cccagtccgt caacatctct gccatccgcg
     4381 cgaatttgta taaggcttct agttcaattt gctgagtgct tttgccgttg actgactcaa
     4441 tgatgaggcg ctgcttttct tgtgcgacag ccctctcttg ttgaagtttg ctttgttctt
     4501 ctaaagacac cttcgttgat aaaatactgg aagcagtcaa acttgccaga acctcaagct
     4561 tttcttgaat cttgtcgatt tcatcagcaa gcacttcgag cccttcctga ttcgcacctg
     4621 ctttattcaa agacttcata aaaggttcca gaaccaactt gtgtccgaaa atcaacttat
     4681 tcaacatggt caaataagca accttcaaat cttcatccgt aattggtaat gcatcgcagc
     4741 tatctttatt tttaagatgc cccgtacact tccactgaac cttcttcgta ttacctttcc
     4801 aataagtgtg aacccgcttg aatttgctgc cgcatttatc acagatgatg ttgcctgaaa
     4861 atgcgtatcg cttggtgtac tttttcccgt caccagtatt ccctttggcc tcagcttttc
     4921 tccgaatgag ttcttgaact cggtcgtaat cttctttaga tactatgggt tcgtgattgt
     4981 ttttgatctg gtacattggc aattcgccct tgtttatgcg tcgtttgtac tggtcatcaa
     5041 caaaggtttg ttgcatcgtc aaagtcccgg tataaacttc atttttcaaa atacttctga
     5101 ctgatgttgc ttgccaattt gccccacgtt tagtgaattg ttttccatcg tttaaccgct
     5161 tggcaatcat tccagtcgaa atgccatcta atgcccactg gaacatttgt ttaacaactt
     5221 ttgcttcagc tgcaacgatg tgaaaatcat agcctttccg cttgtagcca tacggcgggt
     5281 aagctttgaa tttcccttta gacatccggt ttcgaatcgc ccacttcaag ttttctgaat
     5341 gggaatagga ttcactttct gccaagcttg ataaaattga aagcaacaac tcgctttcca
     5401 tatcaccagt attaatgttt tccttttcaa aaaagatggt gacaccaata tcagttagct
     5461 gccgaaccag attcaagcag tcaactgtat ttcttgaaaa tctggaaata gactttgtca
     5521 gaatcaaatc aatctttcca agctctgcat ccttaatcaa tcgttttaaa ccatcacgtt
     5581 ttacagtctt ggttccagaa atcccttcat cataataaac accgacatag tcccattgtt
     5641 catggttctt gatgtgattt tcatagtgag taatctgggt ttgtaaactt gccagctgtt
     5701 cattacttga tgttgataca cgagcgtatg ctgccacctt tagtttggaa gcttccggca
     5761 actctggacg tgccaattta tgtatcgttt tcatcagctc aactcctttc cattaggtga
     5821 catgttagct ctaaagtgcc gttatatcaa cgtttcagac cattatcctt gataaaaaag
     5881 gattgaatgt ttcccggttt ttattgtcga ttttgacgta ttcttccagg ttgattttac
     5941 cggtaacaag taagttcaga aggatctgct gtgaaatgta ataggctgct tcatctgaat
     6001 agttaatttc tgttttcttg ttagtcattg ctttcgcctc ctcaagatac aggcgaagaa
     6061 aatccatgaa agtaaaccca atcacaaatt ttcctttgcc tttcatccat atgcgaaaga
     6121 aataaccgac tcgaacccac aaaacgaaaa aagcctgcaa gaatgaaacg aatcactctt
     6181 gcaggcttct aagtttgttt atatatagta ggaattattc tttgtaaatt ttgacttttt
     6241 acttcccaaa catcaaataa gcctttacaa gcttcatcca ataaataaca atataaatat
     6301 caattgtggg aaggagaaag ttatgagtag ttttgatatg gaagggttag aaaaagaata
     6361 tgaacatttt tgcaaatctg cgacaacaaa ggaactggag gaatttgatg aaagcttcaa
     6421 acaagaattt gaaaagtgct ttaaaaccat agaggctgaa gaagctttat accagttttt
     6481 acaagccaat ataaacgcga tgaataatcg tatggaatca atagaatcgc ctcttttctg
     6541 catcaattac aaagagcagg tatttcagtt aagaaatcat ttaatcctaa tgcaaaatgc
     6601 tgtaaacacc taccttaaag aaaactagac gaaaaaaagg ccccacaata aaacttgtga
     6661 ggctgttttg aattataaag acgtcaaact acttattgaa aattttgtag ctcgttggaa
     6721 ccatcgtgaa gttttcttca gtaattgggc catcataaat tttgattttg caaacttttg
     6781 acgccacatc aaggtcgtga acaaagtaaa ttttatatgg tgtatctcca agggaggcag
     6841 caacctcatt agtactcatt tcaaatccgt cagagaagtt tgtttttgaa gctttcactt
     6901 caatatggat ttcattacct gattggtcaa acgcacaaat atcatagcca tgaccatctc
     6961 cttcagtttt agaaacatgt tcaggaagtt ttgttttgtt cttttctgct tcctgaatga
     7021 ggaaatcaag tacgatttct tcgcctagag cacccgtttt ttgttttgcc ttgttgacac
     7081 gctcccaatc agtatggcgt ccagtgaatt ttcgtttctg agtcccattc gtgcgatttg
     7141 gtcccacttc atcaacaaga acagtaaatc cttttgaact ttcttgctca atttcttcaa
     7201 cttcctcgtt tgtaagatcc agttcagacg gcaacgattg tgtatcatcg attgggataa
     7261 gatgacgatt ttcaatcaat gcaacaaaat cggcataggt aatttcattc attccatact
     7321 gatcccagaa gtaggcccat gaattcggct ttttacgctt ctttttgcta ggtgtattcc
     7381 aatcgaagtt ttcaataaat gaatcacctt gtttaattgc cggttcgatt gtaaatgcgt
     7441 gtgtaattac tgccgtaaca tttccagctt catcaggttc agaaatgctc tcaacgtatc
     7501 cgcccccgta aatttcccac ttacggtctt tagtaatttt ccccggatga cgattcaaaa
     7561 caaccgcgcc ggctttcaat ttaccatatt ggcgcatgtt ccaaccgtaa cttttagcat
     7621 catcagcgta tttcaactga ccatcatctt ctgttttccc gtgatctaaa atgaaagctt
     7681 gttggctttc tgttggttct ggtaacaaat ctttaaacgt aaccatatcg atacctcttt
     7741 ctataattca atcggaacaa atttatgttc tggttgaatt aataataaca cgttcgatat
     7801 tttaagtcaa caatccggac ataattttgt tccgattaaa tttatgaaat caaaatataa
     7861 tcgtcagatt ccgtcttacc agtattctta ccgacagcac aatatccaat cgggcaacga
     7921 gcccataccc aaccatccgc aatcgtcatc ttctctgact tcggaagcgt ccaagttgcg
     7981 cctttattgt atgaaccaac aaccgcacca cttaaacttg gcttgttgcg aacgttgaga
     8041 ccactcactt gaaccacaag agttttcacg ttggatgttg atgacccaga tggataaact
     8101 tgattaccgt tctggtcgaa caccttataa ccagaatggc tatctgcagc ccgcttcgca
     8161 ttgttcaagt ccttataggc accaatttgt gacttggcgt cagcccatga tttgcgaaca
     8221 cgatacagac cactagctgg cgtgcttggg gttgatgaac cattattaat cgcattggct
     8281 ttatccgcaa tctcctgcag tttaccaagc aactgtcccg gacatgatgt ggccgaaaaa
     8341 tgtgagtgag ggaagatatt cttgttcacc accaatttcc ctagaccata acgtttggca
     8401 atatctgcac acaactttgc acttgcatca atagttgctt gattgatacc ccagttcggt
     8461 gatccagtgc tattgcagtg ctcaatcccg atacttgaga tattggcgtt ccaattacca
     8521 gcgtgccaag ctgtgtttcc ttcgtccacg tatgctcgaa tttctccatt ttttccaata
     8581 ccgtagtgtg ctgacgcctc acgcgttttc caaatatcag gaacaatatc atagttggtg
     8641 gttgccatgt gatgaatcac tatcttatct actttggcac cactacgacc attcgagtaa
     8701 tggctaaatg actgaaatcc atattttgtt gctagattac taattcccat tttctgtttc
     8761 ctccttcttt tcgtgtaact gctgcaagat ttcacgcaac ttttccggca ctggcagtcc
     8821 aatcgccacg gcattctcca gcagtgaaat tccttcgttt gagatataga agaaaatgac
     8881 cgcatcacgc agtacatttc cacctttgat gacttccaca tcaagcatat gagccacacc
     8941 aaccaaaaga aaaatcatga ttttcttagt gatgcccttt gccccaatct tgctggatag
     9001 tttcttttcg ttgattgcac gaagcagacc agttacatag tccactacca caaaagttac
     9061 aagcgtataa atcaaaccat caattcctcc cacataccat ccaatcactc ctccaccacc
     9121 tgcgatggcg agttgaatcc aattccacac ttctttcatt tacaaatcct ccaattgttc
     9181 taaaattaaa tcgagcattt cagcatcttt ccctgaaatg gtgacttcac tattttcaaa
     9241 tccagcaacc aataaaggca gcttgtcttg aagttcatcc acgttgatgt gggtttcttc
     9301 gttgagcaag tctgcctttt ctttgtgata ttcagttgcg gtttctggga tgagtgaaaa
     9361 ggcaccgtca tgttggacca aattgccgtg ctcatctttc tttccgtatt tttgaagcaa
     9421 gtctacctcg tcttgataga gttcatcaac cttcacctgg atgagttttg caagcttcgt
     9481 tcgcactcgg ctaatctttg gtggtaagtc aaaacctttt aagaaactca tcacgtccac
     9541 caaatcttca tttctaattt tgagcattat ctatcttctc ctttaaatca ccaatctgga
     9601 cacaaagatc atccacgacc atcacaagca aatcaaccat atccgtccca actttcattg
     9661 gctttggaat atctgcctcg ataggttcag ttactatcac ttcttctttt tcttcgttca
     9721 tggcggcctc ctaactgtat gtggtcattg aattaaacga ctgcggattg taccaaccac
     9781 tcgctgtacc gttgctactg aaaccggttg gtaaagcgat tgttcgtccc gcaagtttgg
     9841 cacagatttc acggatgact ccaaagtcaa cccacttccc atcatctccg agcatcagat
     9901 tagaaccgtt gaatgcaata ccagcgctgc cggaacttga gccaatctta gtataaaggg
     9961 aactactaaa tgtaatccca ccaacagata tttgatgttg tgtccctata cgaagcattg
    10021 atggtaaata aatgtcgtcg tcaaagtgga accccttctc cataccagac tgcaatgttc
    10081 tcgcatacca agaaagcttc tgggtgtaga cactcgcaga ggaggaatct ttatgagccc
    10141 atgacatata ctctgttcca tactcaagat caaataccaa tccacgccaa tcagagtgac
    10201 cactgatgtt atttgcacca attgttccga ctgtcgttcc accacgtcga atggtcattc
    10261 cctgcgagtt tatttcagtc acaagagtgc ttcctgaata atatccaatc gtagttcctg
    10321 tgattcgaat ggacgaactc gcagtcaatg ctgttaggat attagcagca atcttatcag
    10381 ccgtaatcga gttcgcgcca atacgggctg ccgacaaagt tcctgtggta atcttcgccg
    10441 catcaatgct tgcaattttt gcactttgaa ttgtggcatc tgcaatcttt gcgtttgtga
    10501 tggccgcatt ttggatcatg gcagaactaa tcactgcact atcaatggtt gtttgaccag
    10561 tgatgtgcac cttgtttcca gcaatcaaaa tactttcagg tgagatattg atttgattaa
    10621 tcacgtctcc tttaccaact cgtaaattga ttaagtcttt ctgcatgaga attgctgaat
    10681 ataatgtgtt ggcattggta tagtcttgcc ttgtggtcgt aggataaata tttccttcat
    10741 attcatcggg tttgatgctc gtgttcactc ggactcgcat tttcaatgtg ccactcggga
    10801 ctgtaattgc tccgctcgaa ccagtataag agatttgatg ataacttgaa ccatccgacg
    10861 ggtttagagt aaaaggtgtg ttgcttgaat tataaatccc agcatatgtg ccagtcttaa
    10921 ctgaaaagaa agctagatac acgctgtcag tagtaatccc tgatatctgc tcagccgtta
    10981 acgtccaaga ataagtggca ttgctgctac ttgtaacggt cgaaatctgt tcccaagcac
    11041 cacctgtata aagcaagaca atattatctg atgtatcctt gcgaccattc cacgaaatta
    11101 cactcggagc gactttgcct tccaatggca atttgtaaat gaccagataa tctcctgaat
    11161 ttgaataggg gtttgttgtc acgccacttg taatactcat cgattcaacg ttcttactta
    11221 ctagataact cgcaatggtc gcattagtat aagttcccgc cgttagggtt tcactaaatt
    11281 gttttgccgt tgggacaaaa tcaatgtaag gtaaatccat gccgtagtaa tgatagtaga
    11341 aagcagcttg agttccgtca cgcagttgcc cgatatactt atctcccact ttgacatcaa
    11401 ggaactcact tcggagataa ttggttgcgg acacttctga accgtcagtg gtactcaaac
    11461 ttccttgttc ccattgattg actttaaatg gaacgccgat tgcttctttg gtttctgaaa
    11521 cacttaacag taaggcatca gtggtcgctg taagttttac gacgttactt atctcggata
    11581 aatcaacata gccatttgtc acatccgttt tcaacatata gcaactgatg gtttctggtg
    11641 catcactagt tgttaaacga atgcgaataa aagcggcact gcttggaaca gtaagcggtg
    11701 tggtggtgtt tccaaattgg gtgtacgata aaaaggactt atcctctttg taataaagca
    11761 gataagccgt tgcgtaagta gagtaaagtt tcccacccac aaatgtttga agtagatact
    11821 tttcgccatt tctaactctt gtaaaacctg agcgaacgta tccaccatag gtattttctt
    11881 ttccatcatt tgtattcaga ccaccaactg cccactgcaa atctgcaatg ctcataccgg
    11941 ctaaaacatt gaccgcttta tttgttttag aaagagatag ctcaaatcta tcagcatttt
    12001 gggtgatggt gctttctgca gttgtaatac gccctgtcag attatcaacc gtcgattgac
    12061 ttgccttact agcaatctgc ccagcttgca ctgtcagagt tgattcagct gtgattactc
    12121 gtccttcaag agttgacata ccactcgcca ctaatgaaat cttgctagta ttctgttcaa
    12181 cggatgtagt aagcgtttca acacttgcac ctacactttc agctgcattc aaagcattca
    12241 cagcatcgtt aaatgcttga ttggcctttt cagtggcagt attggcatcc ttttgtgctt
    12301 gttccacggc ttgctttact tcttctgccg tcatatctgt cataagtgga taccactgag
    12361 tgacgccatc acggatttca tacacccaca tttcaaactt gtcaccattt tctttgtacc
    12421 aaacgtcacc gagtttcgga ttatttggag ttgtcgggcc atagaagttg gtgtttttac
    12481 catttgccga actcaatgcg taattggcat cgtctttggc ttgctccgcc ttgttgtcca
    12541 cccggttgat gtccttcgcc acatccgtga attttggcgt gatgtttccg agtgttactg
    12601 agatatacgc tttcagaaat ggatcgtatt tgtatgagac cattctagct gtgatgttca
    12661 atccatcttc ggcatgaata actgaaacag tatcacccaa attaatcgtt tcaagctgtg
    12721 caaaatcttt atattcttca gttcgctcaa gcggggcaaa ctccacatca taggtggctt
    12781 ttggcaaatc gaccttgtta gctgagtatt ctaacccagc caatctgcga agttcatcat
    12841 aggcctcggc ttgagtaaac tcatcctctt catcaccaac tttgacgttg tcgtacttga
    12901 tgactttgat tttaggctgg acgtattggt tgatgtttgg actgtccacg tatttttcgg
    12961 gaagaaatag tccatcatac ccttgtggca taatgcgagt aacaattcca gagtaatcaa
    13021 catcagcttt atatcctgtc agattcttct tgtctcggat ttgtacgccg ttgttactgc
    13081 cacgagtcgt taacattgaa atcgtgaagt tatcgcgtac aatttcacca ccataactgc
    13141 tttgaaaacc attctctagg tcactatcaa gcaacacctc tacaggattt agtcgcacca
    13201 agcggacatt attgaccgca ctgatgtttg atgtaccggt aaaacggtgc atgaattgag
    13261 ttgcaccaag cagttgtgtc aaagctcccg acccgttctt gttgacgata taagtatctt
    13321 caataaagtt tgtggctagg tcatagaaaa tatgatgagc tactatgtgc aaaaaaccac
    13381 caagtgcatc atctcgttca acaatcctga aaagctggtc ggacagttct ggtactggtg
    13441 ctttcacaat catttctggc aacaattcct cactgtgtgg agcatgaatt ggataatcga
    13501 attcaaagga aaagagtccg ttcaattcct cactcaccac ggggttcaga atgttgccat
    13561 caaggacagc gagaccgtta tgattgaaat ttgattctgt tttgttgtaa agcgtaatca
    13621 taagtatcgc cacctcggtt ctatttctac tttggttata cctgtaccaa gagtcacagt
    13681 attactgccg actcgaaact caggaaagtc acctgtcatg tattgattgg ctgccacatc
    13741 cccgtaaaag cattctttca aatccgaatc tagtgtcagg ttgccattcg tcagatttaa
    13801 cacgataggc ttgccattta tcgtgagtgt cctagttcca ttgccgtaga ccttaatctt
    13861 cggcaatgag taaatgttgc cgggattatt gaccgttcct gacgtggttc gattgatagt
    13921 cgctacattg gtcttataac gaaaaggcga acaggttaac tccatttcaa actcccacat
    13981 ggaagataaa atctgagtta gtccgctcgc cttagcatac ttgattgggt aaaacacgtt
    14041 tggttcattt gaaaatgcga tagttttagc gtttaggatt tgtggcaaaa ttatctgcca
    14101 gctagtccaa acatcagatt tccaaatcac taccttaaaa ccgaaagtca catctttcca
    14161 gcctttcaga atggtcaaat ctccttcacg tccatcaatc tcaatactat caatgacacg
    14221 ttccgtcact ggaataactg gcacttccgt aattcgcaaa cctaaatcag tccgtgaatt
    14281 aattgttccg tctaaaataa atgcgttcaa ttgaccaccg cccatctaaa taactaaaaa
    14341 aggaccagac aacaatgtct aatcctaaag tttataatct atcatcctat tcggttgttt
    14401 cttccagccc tattttcttg gtaatcgcta aatacgcgta tacaaccagc acgccggaaa
    14461 ttccatatat tgcatcgatt agatctggtg tattcagaaa agtcatcatc gtttcacata
    14521 caatatttgc aacaatcaga ccagctccca tcgcagtaag aacccttaaa ttcgttccca
    14581 taagcaacca aagatagcct atcaccaaat agaaaagaaa gcttaatgcg aagtttgaaa
    14641 tgtgagaatt aatctgattg ttgatgaata caaaaccatg atttatgccg ctataccatc
    14701 ttactgccgt taaaaataat ccaatagcaa gcagtagaat atatcctatc agtgcaggtt
    14761 tctttgcttt aaatttatgc ttcaacatat gattaccacc atctcttcag taaacactcg
    14821 ttagttatag tatacagtca aaatgtgaaa atccaacact aatatgctac acgtctacga
    14881 ttcaacgtcg ccaagctctt atcaatttct ggagcaagtt tgccgaccaa tgttccatca
    14941 ttcaagacga ctttaatgtc cattgcttta agtagcgttg gcaacatttc actcgtgatg
    15001 tttgttaagg catcaacctt agcacctaaa tcaagcatcg agaatgtgct atcaacggca
    15061 gcgtggatat cggtatcaat atcaaagctt ggcatataaa gttttgttgg aatagctttc
    15121 tgcatcgttt tagaaacacc agtcatttcc ttatcaaaac ctacgccaat cccttgtgcc
    15181 atgtacttcc cgatttcatc acgaaacagt gttgatggtg aatgaatacc aaagaagtcc
    15241 ttgatgttgt ctactacacc gccaaagaat cctgaaatct tgttgcgtaa ccaacttcca
    15301 gcatcagcga taccattcca caatcctgta atcaagttct tcccgatagt tcccatagtt
    15361 gaaacatact caccgaaagc attcgcaagc cctgtgatga tttgcggtac tgctttgaca
    15421 atggcagaaa tgattgctgg taagttttga actaatgcca caagcaactt cacaccagca
    15481 ttgatgattt tgtcgatgtt gtttaccaaa gttcctgtta ttccgttgat aatttgcggc
    15541 atggcattcg caatcgttgt gataatttgc ggtaacgcac caatcaatgc cacaagtaac
    15601 tgaatacctg cgtcaatcaa ctgagggatt gccccaatca cggcattgat gatattttga
    15661 ataatcactg gaatagctgc aacaatcgca ttgatgatat ccggcaaagc tgtcacaagt
    15721 gatgtcaaaa gttgaatacc agtctgaatg atttgtggaa tagcactgat aataaagttc
    15781 acgattcctg taataatctg tggcagactt tcaatcagta ttggaagtgc tgtgataagt
    15841 ccttgggcaa gtcccatgat aagttgtaaa gcagcatcca acagtaatgg caagttttca
    15901 atcaagccgt tcacaatcgt tatgattgcc tgtactgtaa ttggaataag ttctggcagc
    15961 gcttcgccta gtccttggat aagtgttgtc acaagttgaa aggcagcgtc aatcagtaaa
    16021 ggtaagttct caataagtgt ttgaacaatc gttaaaacgg cttgaaccac cactggaata
    16081 agtgtgggaa gtaagccaag tatcgtttca agcacttgtg agaataatga ggttactgct
    16141 tctaagagtg ttggcaataa ctgccccaat gcttgaataa tggcatccac cactgccggt
    16201 aaagccgaca cgatattttg aatgaccggt agaatattct tgatgacgtt ttgaaattgt
    16261 tccaccacat tgccaatcaa cataccaatg tcagcgctgc tgttaccaag tccagccatc
    16321 aagtttgaga ttgcagactt cataccgtca atcgaaccac ttatcgtctc actcgcttcc
    16381 ttggcggttg taccagtgat gcccatttcg gtttgaataa cgtgaatcgc ttcagtcaca
    16441 tcagcgaagt tcgagatatc atatttgaca ccagaaatct tctcagcctc ttggagcaaa
    16501 cgttccattt cggacttcgt gcccccaaaa cctaatttca agttgtccag catcccgtaa
    16561 ttttgcttgg cgaaaccttg ataggcgttc tggatggact caatatccgt ccccatcttg
    16621 tttgcattat cgctcatatc agtaacagct tgatccgcaa ctcctgcggc tttcttggta
    16681 tcaccaccga gagatgaaat caaggcagct gagaaacctg tgacagtttc catgtattca
    16741 ttggctgaca ttccggcagt tttgaaagca ttgtcggcat tcttttgaac ggtggatgac
    16801 gcatccttga aaagagtatc caccccaccg actaattgtt catagtccgc gtaagcagat
    16861 accacttgtt ttcctagctc cacggcagca gcgccagcag caagtgccac tgtacccatt
    16921 gctaccccga taccttttag aacgccaccg aacttttcaa atttacttcc agacttttca
    16981 gcctcgtcac ctgtatcctt gagttcgttg ccaagctcat cagttttctt agcagcatca
    17041 acttcttcag tacccatggc gtcaattgcc ttctcgttgt tagcaacttc acgctccata
    17101 ttattgagtt gggcttttgc attgtttaat tgcactgtcc aattctgagt acgcttatcg
    17161 ttttcaccaa aactgtcact agcgttcttc aaagcatttt caagggtggt gattttttct
    17221 ttctgggcat caatcgattt gtttaagact tggttgcgag cagacagtga gctgatgctt
    17281 ttatcgtttt tatcaaattc ggatgatacc agtttcattt ctgaacctag caccttgaat
    17341 gattggttga tttcacgcaa cgagttctta aactcctttt caccttcaac gccaatcttt
    17401 aaaccaaaat tatctgccaa ataaatcacc accttccctt aaattccact cggtatcaca
    17461 tcatcaatga ataactcaac tttcggcttg ctaatcccag taaattgctt atgacactcc
    17521 cataaatcca aaaacagtcc aagcgagcag taccagaaat catctgacgt catacccatt
    17581 tgaactgtcc catagtaata caggcgagta aatgtttctt catcacttac tcgcctgctt
    17641 tgttttttga atctgtttca ctttcgatat tgcgagctgt gcccttgaac attgcttcag
    17701 tgattgctga tttatattca gccaactgca acggactcgt catcaactcc acatattcag
    17761 tcgtgagttc atctttcttt tctgtgttct tcaagttgta aatcttgatg gactgattcg
    17821 ccaacagcgt aatcaaccag atgatttcat caagcgccaa ttcaaagttc tcggatttca
    17881 acaatttctc accaaggtta tcaagcccac cgtagtgact ggcgatttct ttcgtggctt
    17941 ttgttgtcaa aatcagttca tattcatctc cgccaaggct aatcttggca ctgcgttctt
    18001 cactcattgt tgcctcctag tctgctgtga aatctggttc ataaacactc gtgtaccatc
    18061 cattgatagt tgtttgttca actcccgtat cgttttcatc aacttctgct ttccatggat
    18121 gcttaccact agcatctaac ttgttacgac gcatgactgt accctcaatt gttggtgttg
    18181 agaatgtaat gtcgtcacct tttgttgcta ggcttgtgct gggcacggca aacttcactc
    18241 ggtacaacca caaatatttg tatttgccat tcgatttacg agcacggaaa ccaatcgcaa
    18301 ctggtgtgcc accatcttcg gttgtcgaaa ttaagacacc gttttgatcc acggttgcac
    18361 caacaagcac tgccgccaca gcacgtccaa tatcatcaac accgagcgtt aactttccat
    18421 ttttaaattc tttcactact tctgatgcac catcatcggc gtataaagtt gcttcagcaa
    18481 gttccaccga aagctcagct gagattgctt tcgcaagcat agcgggtgtg ccgtatgttt
    18541 cttcgccagt agttgccgac tctgtaatcg gtgcataata taatttatcc aatccaatcg
    18601 ttgccatttt cagacctcca tttcataatg cttggcgaca tcaatcgcca catgatagta
    18661 atttgtatct ttttcaaatc ctacaaataa tcgttcagta atcgtaaact cgtgtttcaa
    18721 caagttctga accagttgtt tttgcgtgtt cagatagttg cctttcacaa acaaggaaat
    18781 gcgaacttcc tgtacatcaa cggttggaaa gttatccccg taaacatcga acctgtcagc
    18841 gagtggtgtc agcacacaat acttgtctgg cgctttatca ctaaactcgc ccgtttcaac
    18901 aggaatatga agtgtggcaa aaagactgtt caattcctct aaaatgttca aatgccgttt
    18961 acctcacttt ccatttttga tttcattgtt tcaatgcatg acttgcgact ttgtgatttt
    19021 gcaggtttca agaatggctt tgctggctga ccgtgttttc catattcgag aatgttcgca
    19081 atcttagcat ttgaactgcc atcgattcga ggctcatcaa aaccaacctt gatgttccag
    19141 ttgccgcttt tatcttgacg agcttgtgtg ataccaaggg ctgattcaag ttcaccagtt
    19201 gaacgacttg gtacttttgt atttgagcca acaacggcag ttaagttact tcggactttg
    19261 ccttccacca cttcagcacc actttctaaa accttgggga tgatttcatc tgtcttgttt
    19321 tctagttttg aaacctttaa caagaaatct tctggcatct tcatcaatgc tttagccatc
    19381 cgcactcacc tccatttcag tagtcagcat ttcaaggtac atattgcgat ttcggacatt
    19441 ctccaccgac acaatctgat accttttctg atctgaaatg acaaccatct ctgtcgtaac
    19501 ctctagatta ggaatataac gaatccgaaa caggcaggtc gctgaactaa aagtcgccat
    19561 gtttacccat ttctcagtcg cattcttttc ctctttgtat gcatggacag ctgcaagaac
    19621 ttcatcttgt ttcactgaaa atccagcgct atccttttgg ttcgtcactt taacgatttg
    19681 tattcgttga ttcatctttc caaaactcat acttgccacc gcctatcgag cctgagtaac
    19741 agattgaccg tttcccagac ttgcttactg gcattgacgt tatccgcaaa gaagccacca
    19801 gttgaaccat cacggctttc gtaaaagtta ctggctaaca caataaccgc ctgttcagtg
    19861 gttggcggca tttcatgttc tgaatagtag ccagactcaa tatgttgata gctctcagca
    19921 taagaaagag cggtcagaat caatcgttcc aacagctcat catcttcgtt atgctcaagt
    19981 atgaggtttg ccttgacctt atttagtaag tcactcatag gctactcctt aagatgaggc
    20041 agatgctttc atcttcaaga tttgcactgc ttcaggtaac acaagtttac cgtccacacg
    20101 ttctttagct aagaaaccaa ccatgccatt acctgcaaag agttcacgca actggtcgaa
    20161 tgaacgcacg ccacggtcgc caatgttata gtagctgaaa tcaccaaaag caatgactgg
    20221 cgcacctgcc ttaacggttg gaacaaactc cgaagtataa actggataac cgagcagacg
    20281 gtcaggttca cctgctacca aagctggctg ccacatgtag gcaccattgt tgtctttcaa
    20341 cttacgaagc aagctgatgg tttggtcgtt catgataaac ttcgcgtttt tacgatatgg
    20401 acgtttcaaa gcatacacaa ggttcaccac atcatctgcc gtaactgcac tttgagtatt
    20461 agttgtaatg gcaacttgtc cgccacctgt tgctgcaaaa ataccgagtg gtttaccaac
    20521 accgtcacca ttcaagaacg catcttcttc cgcgtttgct aaggctttgg tgaattggtc
    20581 gatgatatag ttttcaaggt taaaggcgtt gtcataaagc aattcatcag tcaccttgat
    20641 tgccacatga agtttgtgag catcaagcaa gatttgatcg aacgttgcat ccccaaagct
    20701 caactcgcca ccttcttcaa tccatgaagc ggctggttta gaacctgcaa tattaatctt
    20761 gtgttcacca cttgtcgtaa ttgttgtcgc aagtgtacgc atgatgtttt cagaagtcat
    20821 gccgtcaatc aaacgtgagt catattcttc tggcaccaaa taaccgcctg ccgtatccac
    20881 accttcttga agaacatttg aaacatgtcg gaagtttgag cgcaaggctt caagtacacc
    20941 ttctttatat tcagctgaag cacgtcctgt tttcttaggt tccattttat tttcagtagt
    21001 cataggttgc gaagttaatg gttggctcat tggcttttca agttcacgat ccatcgcttc
    21061 cattgactgc atacgttcaa tttccatcgt aaagttcttc acttttgttt ccatttccgc
    21121 ataagtcttg gcatcttctt cagacatcaa gccatcttta tcttgctttg cttctacaaa
    21181 agcctttgct ccgttccaag cttgtgcacg tttttccatc aattcttgaa ttttgttcat
    21241 gggttagttc ctccaatttt tcattaaatt aagccgactc attaacgaat cggctgggat
    21301 agtgttatta atttcttctc gttcctttgg ttcaggtggt tcaatacgac acttcttagc
    21361 aatcgctgaa gtcagtgaat tagttacttt gacttccgag tacatcgaat tattaaccgg
    21421 aatatccggg tcaatctcac cgatgatttc atcggcaaat cctaggtgaa cagctttatt
    21481 tgcgtccatc cacgtctccg catccatcat atgtgccagt tgcacacgcg ataaattcgt
    21541 tttacgttca taggcgttga taatacttgc tttcacttca tcaagcatgg caatggcttt
    21601 ctccatatca actttttcgc caaacgcaac cgctgacgga ttgtgaatca tcaccatcgc
    21661 cacaggactc atgagtacct tgtcacctgc cattgcaata acacttgcgg ccgaagcggc
    21721 gatgccgtca atcttgactg tgactttacc tttgtagtca gcaagcatgt tgtaaatact
    21781 tgcggcagcg acacaatcgc caccacccga attaatccag actgtaacgt tacctccacc
    21841 tgaatttaac tcatctttga aaatctgcgg tgtgacttca tcatcaaacc agctctcctc
    21901 agcgattggt ccgttgaggt agagggttct ctctaggatt tcctcttggt tgtcttgatt
    21961 cagaaccgtt tgattcttcc acttccaaaa cttcttcatc ttgttcctcg ctttcttctt
    22021 tgtttacgcc ataagctgcc ccggcttgtt caattggcag catattgcca ttgaagtaat
    22081 atttcgcacc agcaccacca gggattgggt tcaagtcctc aagttcacgc acatcatcag
    22141 gagataggaa cccattttga ataccggttg catagccttg ttgacgtgtg gcatagtcac
    22201 cacgcaataa gccatccaca ttaaacttta tgaaatactt ttctttttca tcgcgactta
    22261 acagtgattt attaagtgct tgttcccaac gattgagcca tggtgataat gagaacttca
    22321 caaaatccaa actcaaatgt tcaacgtttg agaaggtcgc gtggtctaag tcgccaacca
    22381 tgtgaagggg cactcggaat atccgtgcga tttcttccag ttgaaactta cgtgtttcaa
    22441 ggaactgggc ttgttctggt gacatggaaa taggcgtata cttcatgcct tcttcaagta
    22501 ctgcaacctt ggccgcattt gaactgccac caaatgtcgc attccaactt tcacgaagct
    22561 ttcctgggtc tttcaaagtt ccaggatgtt ctaggacacc accaggattg gcaccattgg
    22621 caaaaaattt agaaccatat tcctctgttg caatcgcaag cccgatagca ttcttagcca
    22681 tggcaattgg tgaataacca accaagccat caaagccaag gccaggaatg tgaagcacgt
    22741 cagcttttga aagtctgact tgcccgacat cagcttgata taaataatag atttccttgt
    22801 tgtcatcgcg attaaccgtc atcttatctg gcattaaagg atacaaagct acaacttcac
    22861 ctttgccatt tcggataatt tgcgcatagg cgttacccca aagtagcagg tgagtcatta
    22921 aggtttcacg aaatacaaat gatgtcattt ccgaatttgg ttcatcatgc agcaagaaat
    22981 acaacgaatg tttcattgct ttatctttgc cgccagcatc tcggtattgg taaaagtgta
    23041 atggcaatcc tgccacggct tctgatagca cgcgcacaca tgagtacaca gccgtcatct
    23101 gcatagctgt ttgttcggtc acgcttttac cagctgatga gccgccaaac ataaatcggt
    23161 atggattcat gatggtttga tttgttggtt tatcgcgtga tttgaatagt gattttatta
    23221 tgcccattga ttgctccttt caaaatttga gtatcaaaaa agcacctacg atttgtagat
    23281 gctgtgttca cattagatat aaaaccgact tcctcttgac aaaattatct caattgcatt
    23341 atttaaaaag gtatttactt cctctaaaca tttcgatttt tcgtcaggtg ttaaggattt
    23401 tctttgataa ggaaagaatg aactacttgg tgcaagtttt ttaaacgtca tataatcttc
    23461 atcatcgaag aaatcttgta aataatttag ttctataaaa cttttccaat ctttgaattt
    23521 agagaaatag tctttattat taacgataaa tttatcagcg ttagttgatg aaccatatcg
    23581 tttatctctt acatcagaat aaaataaatc aggaaagtca tgatacggcc ctttaccttt
    23641 aacaccattg agagaaggtt ttgatacata tagtggcata aaatttgcaa gagtatgact
    23701 taaatcatag aattttacaa atttcacttt catctcttcg gtgatatcat cggaatataa
    23761 aatgactttc aaacgcccat aatgactatc tggcattgaa aagttctttt gattatagca
    23821 tttcaataac cgtatcgttg caccagcaat tgtgttgaaa gaaataatcg tttctcccct
    23881 ataattccca attccttcag catactttgt caaatcttca gttagaattt ttccccacca
    23941 ttttctataa aacagtctag aaattaatgt gttgtccggg tctgaagata ttttatctgg
    24001 tgctacttga atcaaatatt cattcataca catacacctc caaagacaat tatatcaatg
    24061 gattgtaata aaagctcata tttcacagaa acaacaatcc cctatcatca taaacactgg
    24121 caccattatc atttccacaa cgaattgcac gatccagagc cataattgtc gcgatggcac
    24181 catcaatctt ctcagtggat ttttctttat cagctttgat attacctgcc gggtcagttc
    24241 ggacgaaaat gttgtccata ttccaacgaa gaaccggatg accgccatgg gctaatttct
    24301 gttcaagagt caacttcatc aactctttag tagccgggct catatccttg aaaccttgac
    24361 cgaaaggaac tacagtaaat cccatacctt ctaagttttg aaccatttga actgctcccc
    24421 aacggtcaaa ggcaatctcc cggatattgt atttctcacc cagttcctca ataaattttt
    24481 caatgaaacc atagtggact acgtttccct cagtggtttg tatgtacccc tgcttggccc
    24541 acaaatcata attcacatgg tcgcgtttaa ctcgtaagtc caaggtatct tctggcaccc
    24601 agtaatatgg cagtatcaca aacttgtcat cctcatcttc aggtggaaac accaaaacga
    24661 aggatgtaag gtcggtcgtg ctggaaaggt cgagacctcc gtaacaaact cgacctctca
    24721 aactttcttc atctgcattg aatgaacaag cgtcccattt atccattggc atccaacgca
    24781 ctgcttgttt cacccattgg ttcaggcgaa gctgtctgaa tgagttttct tcagctggat
    24841 tttgtttagc cgaatcacaa gcagccacca ccttttcttc tgcaatcgtt atcccaagtg
    24901 acggattggc tttcttccaa acttctggat cagtccaatc atcatcttgc ttgataccgt
    24961 aaattaacgg atagaatgtt ttatcatgct ttcgaccttc aataatgtcg agagcttttt
    25021 gatgctgctc ataacaaatt gaattggtat cattccctgc cgttgtaatc aaaaagaata
    25081 gcggttgcgt tctagcatcc cctgcccctt tcgtcatgac atcaaacagc tttctgttag
    25141 gttgtgcgtg aagctcatca aatacaaccg cagaactgtt taatccgtgt ttagagtatg
    25201 cttcagctga taatacttga tagaaactat tcgttggctg ataaataatt cgtttttgtg
    25261 aagtcaggat tttcactcgt ttgcttaacg ctgggttctg gcgaatcata tcaacagcta
    25321 cgtcaaatac gattgaagcc tgcgctcgat cagcagcaca gccatatacc tcagcgcgtt
    25381 cttccccatc accgcaagtc aaaagcaaag ccacggcagc cgctaactca ctcttaccat
    25441 tcttcttagg tatttcaaca taagccgtat tgaattgtct gtagccattt gccttaatcg
    25501 ttccgaataa gtcacggata atcttttctt gccaaggcat cagtttgaac ggtttcccga
    25561 accactgacc tttcgtgtgt ttcaacaact caataaaggc cacggcataa tctgcacgtg
    25621 acttatcata atgggagtct ttgtccataa attcagttgg tttgtaatta tcgattggaa
    25681 ctcactccct tctacgcaaa aagcagctgt tgtcagctgc cttaatctct ttattccatc
    25741 atatcggcaa tgaactgaat ggcttcaggg ttgttcttgt tatcccgcag caattgcacc
    25801 agttcttgcc aatcgtagta tccttcttca atgccatctt cattatctgg catgtaaatt
    25861 tgataatcca tcatttatca ccctccaaga taaagcggct gtactcatct ttgtgttctt
    25921 caatgaaaat caccaactcg taaaagtgcc ggtcatgtgc aattcgctgc actgcaatcg
    25981 catcaaacat atttgtcaga ccactttctg caatctcaat gatttgttgc ttaatctttg
    26041 cgttcatttt gaccgtcctt tctgcctgcc tcataggccg caattaaggc atcttccaaa
    26101 caccaaaccg caatgtccaa gaagtcttca cggtcgctgt tgtgggcctt caggtcgcca
    26161 cgttcaggca actgaacgat gtaattcttt gcgatgttgt aaatcttacg tttactcact
    26221 ttcatcttaa agcccctttc tgatgtagat ggtatcttct gtttttgact caaggtcaac
    26281 ccagaccaaa tcgtggttca atatcatgtc cgtcacgtat tgccaagcat cattgaaggt
    26341 ttcaaatcct tctcttctac cgttttgaaa gaccaattcg aagtctggtt tgatgatgtg
    26401 caccacatct tcgccgtata ccgcgttcag tgttgaaccg ttgtcccatt ggataaggat
    26461 tgaaccaatg tcgtcaacac cggttactgt gccttgtgtg cccttcgctg gggcatgtgg
    26521 gtcgttcatc ttgatgagtt ctacacgact accttcaggg taacgttttt tcataccctc
    26581 gatttgttca tgagttggaa acataacttt gtcttccgta ttcttttggt agtctatata
    26641 tcactctaaa gggattttat agcaagtgat acgttattta ttcaggattt tctaggtcat
    26701 taaatgcgat agtctttcca tctcgaatca ctgaaacgtc tgcatcgctt ccagcctgct
    26761 cgatataacg cttaacaatc acatcacaga acttttcatc cagttcgatc gtatggcaaa
    26821 tccggtcggt ctgttcgcaa gcaatcaaag ttgatccgct gccaccaaaa ctatccaaca
    26881 cgatgcagtt gctgagtgac gaattggtaa tcggatatgc caacagcgga attggtttca
    26941 tcgttgggtg gtcaccattc ttctttggtt tatcaaattc ccagatggtc gattccttac
    27001 gtccggtgta ccattgatgt ttgcctttct tcttccagcc atagagcacc ggctcatgct
    27061 gccattggta aggcgaacga ccaagcacta gactctgttt cttccagatg cacgtacctg
    27121 ataaatagaa ccctgcatcc acgaatgctt ttctgaagtt caagccttcc gtatccgcat
    27181 ggaaaacata aatactggcg tcgttggcca tcacactttc catacaagaa aaggcatcat
    27241 acaagaagtt gtaaaatgcc tcgtccttca gattatcatt tttaatcgtt cccgctttac
    27301 cttgatatga gaccccataa ggtggatctg tcaccacaag atttgctagt ttgccgtcca
    27361 tgagtttctc ataagtttca ggttttgttg agtcaccaca aactaaacgg tgacgaccaa
    27421 gcaaccacaa gtcccctgtt ttactgaagg ctggcttttc aagttcctca tctacatcaa
    27481 agtcatcatc ttgggcatca tcattaccgt tgagtaattt atcaagttca tcagtttcaa
    27541 aaccaagcaa atccaaatca aactcttcac cttgcaggtc tgataactca accgccaaca
    27601 tttcatcatc ccaaccggcg ttcattgcta gtcggttatc agcaagtatg tacgcacgct
    27661 tttgtgcctc ggttaggtgt tccacaaaca cgcacggtac ctgcttgata ccttcttctt
    27721 tagccgcaag aatacgtccg tgacctgcaa tcaagttata atccttatca acaatcaccg
    27781 gatttatgaa cccaaactca cgcatagaag cttgaatctg tttcacttga tccttactgt
    27841 gggtacgggc atttctggca taagggacca gcttatcaat atcaactttc tcaaaacgtt
    27901 ctgttgtgtt cattagaaca acccccattc tgctagtttc tcaaacccgc caagctcatt
    27961 cacataatca cgcgcaactt ccacgatttc agaataaggc acgccatcaa tctcagtgtc
    28021 accaatcgca cagcttaact caacgggcat accagtttct tgagctttca agaatgcgta
    28081 gatgttcact gacacatcag cttttgataa gtctttccca tggataccgc cacctgttac
    28141 tccgtctgcc atatcggaac caagcttgcg attagtcgca cctgtatcca catccgggcc
    28201 gccagtccaa tcacctagag ggttgactgt agcgtttgga tatttttgtt ttaactcatt
    28261 tgtctttgcg tggctctgac aaattatcaa gcgttcacca tcaagaatgt actttccatc
    28321 aaatggatgg tcctgatata agtcacgcgc aatttttgag agctgtaact gttcatcagt
    28381 caatggcatc ccacgaaaga ttccattatc gccacaacga atttcagtcg cttgattgtc
    28441 agccaactct ttatcttggg gcacaatcac cacatctttt ttaattttgc ctgcgatacg
    28501 attaatcgct tggctgattt cattcaaatt taaagcagtt gaagtttcaa taatcacatg
    28561 acagatgcca tgaccaatta aaacctcaac tgctatcttt ggattatttt cttctgcgta
    28621 tgccaagtcc acaatggctc cggcaatacg atctgcaact ttatctggat gttgcggatt
    28681 aaccttctct atcataaatt ctgttccttt caaccattct tagaacgtaa caatcgttcc
    28741 atcgcatccg tattcggatc tcctgtgtat tccactgatg cattctcttt tacaatctgt
    28801 tgaatctcat accacgtcac atttgcttgc ttttggaagt tctgcgacat cgccacaaat
    28861 ggggaggcca tgactgcctt ggtagtcggg tgttttccga gcaaaccata ttgactcaga
    28921 gcattttcac attgaacaaa acgcgcaaag ttctgagcgt aggactcaac cattcgtggg
    28981 gctatcaacg tttcgcatcc gcggtctttc aaccattgga acgttgagtt ataaatttca
    29041 tcagcgccta gcacagtgcc atccttctgt tttgcagaaa ggtaagcact tggttcagga
    29101 taatcattgc cctcaagctc tacaccctca ccaatatctt caccaattaa taattcaaca
    29161 tccggtaagt caaattccat ccgcgatgct ttctttccag cagcaacttt atcaacgagt
    29221 ggctgtgagc gttcccctga acgaggtcgt ctgccaccgc gttgtgttcc atcttttgcc
    29281 aaaatttatc acctcttttt cttgtttggg gctatccccc gtttgaactg aacttttttc
    29341 gcgtaagggc ctagcgcgct gtacagttta gaagggttta gggataatta ccgcccctcc
    29401 cccccttgtt atttttcaca tgattgtttt atcttttgtg tttcgtcgag tcatgtgaat
    29461 tttcgagtga catgatttgc agacactcat caaattattt tcatcatgcg taccaccatc
    29521 acttaattcc ttgatgtgat gcacaatctc aaccgaagta aagcgtcctt ccttcagaca
    29581 catctcacat aggggatgtg ctgccacgta ccttgcccgt attttcttcc acgctctgcc
    29641 gtaacgtttg ttgattgccg gatcacgctc gtatttctcg taacgtttcc tcgcaagtgt
    29701 tttatgttca tcacaataag tatcatgcgt acggttcgga caacctggga acttacaggg
    29761 gttagctggt ttcattggca agtggggtca catcctttcg ggcaaaagaa aagcccttga
    29821 aggatttttc ccacaaaggc ttcgagtatt tgttcgatac ttctattgct ggtattgctt
    29881 gagtatcgtt tgtctttttt gacatcttaa tgatatcact aaaggctact gacattcact
    29941 gacacatttc aaatgactat gggttcatct ggtagcttca agttctcaag agctttgcta
    30001 tgccaacgaa agactgtccg cacgtcagcg tgaagatctt cagcaatctt gtcccagctc
    30061 tcttccaaca ggtatcgatt gattaacaca atcttttcgt ccacgtttgt gagttcatca
    30121 atcacgccac gcatttgtcg tttgaggtct acaaacttat caatctctgc atcaatttca
    30181 gcttccaatt ggtaacactt ctcaaggtta cgaacaaatg gtgcctcagt attccgtgtg
    30241 ccacttgaaa tcttttcttc gaatgttgtg gaagaaactg tcaccgccag ttgatagagc
    30301 tccgccttct ctttaatccg gtcgtcaatt cgtttattca aacggtacgc ttgtttcaag
    30361 tattcttttg gtgtcacttg cttccctcca atctagcttt cactgcatca atcaaacatg
    30421 attgtgtttt atcttttcgt ttcaaagcca acatcacatc ttcatcaatc gtatctttcg
    30481 caataatgtg atgaatgacc acagtatcac tttgcccttg tcgccacagc cttgcattgg
    30541 tttgttggta aagttccaaa ctccacgtaa gaccaaacca tacaagtgtt gaaccaccag
    30601 cttgtaaatt caatccatgt cctgcactag ccggatgaat gacagcgatg gggatgctac
    30661 cttcgttcca atcaataatg tcctgagacg ttttgatctg tcgaacgttg aatcgttcct
    30721 tgatacgctc cagatcatgc tggaaccaat aggcaatcag aatcggtttg ccatttgtac
    30781 cttcaatcaa atcttccaaa gcatctaact tctggtcatg gatgtgatga ctcacattaa
    30841 actcatcata gattgctcca ttcgccattt gtagcagttt attcgaaagc accgctgtat
    30901 tggctgcatc aatggtggct tctttcaatt gtagcaccaa gtctgcctta atttcatcgt
    30961 acttttgctt ttctttgagt cccatatcaa ctggcacttc attcatcaca agctctggca
    31021 tatccaagaa atcgactgac ttcattgaaa tcgtgatatc tgaaatcttg tcgtagattt
    31081 cttgttctgc atcttccctt ggtttccaag aataaatctg catgccattt tgcttgtcag
    31141 gtagaaagta tttatctcga taacgtgaaa tataatagcc caatctctcg cccatatcca
    31201 gaaccttgaa ctccgcaaac aaatccatca aaccattact tgaaggtgtt ccggtcaatc
    31261 caacaattcg gtcaaccttg tgtctgactt tcataaagct agtgaaccgt ttagctttta
    31321 agtttttgaa ggacgacagt tcgtcaatca ccaccatatc aaaatcaaac gtaaaacccg
    31381 atttgttaat cagccagtcc aagttctcac gattgatcag atagatgttg gcattatttt
    31441 gtaatgctgc cattcgctga cttggtgttc caagaaccac ggaatacgat aaatctttca
    31501 aatgatccca cttttgaatt tcagttggcc atgttgattg cgtcacgcgc aacggtgcaa
    31561 taattaagac acgttgtatt gtaaagtcat catgcatcaa gtctttaata gctgtcagcg
    31621 ttgtcaccgt tttacccaag cccatgtcca atagaagggc ggcagtttta tgatctttga
    31681 taaaatcaat cgcatatttc tggtagttat gtggtacgaa cttcatcaga catcacctcc
    31741 tattctttct agtaactcag gtatctgatc aatctcatca agcacgaaac actgaaaccc
    31801 taaatctgtc agttgtttca tacgaaaaag ctgtaaagac cgtggcttct tccctggcgc
    31861 cttcacttcg acaaatccaa gtttcccatt tggcataagt accaatcggt caggcacacc
    31921 agcaagcccc ggtgatgtaa acttcgggca aataccacct acccttttga cggctttcac
    31981 tagtgcttgc tcaacctgct tttctctcat agccacaccg tcaaatactc gaaatacgca
    32041 tcacgcaaga tttccaagta ttccttttct gcgcagtgaa tcatcagatg tgcgacttgc
    32101 ttatcgaatg atttcttcca aggatacgtg acatccgaca tggcaaactc gtaaaactca
    32161 tcaaatcgta aatcacgttt accgattcca atttgtaacc actgtttaaa tttcatttag
    32221 atattctcca atctgcaagt atggtgtaac ccgtgaatac gcaatacata actttatata
    32281 ggtggtaatt ttttatccct taagaaaagt tctatataag acctacacag gttacacttt
    32341 cctttaaaat caactattcc aagaactcgg atttgagttg caaaccaaca atcttgtttc
    32401 ctttattagt tcgccggcgc ttaaatccgc ccgcctccag agccgtgtaa aaatcggctg
    32461 aactacgaat gtactcacca gttcgcgtac aaaacgcacg atactctgca tacaactcac
    32521 cagatttttc ttcaaagtca acagcagtct cgcagcactc gtctaagaaa tggccaagcc
    32581 aatcattcgc cgtcttatat tcagaaattg cattagcgac aacttgtgga accgttaaat
    32641 gaaagtctgc ggcgatgact tttttcgcac cgtcaaggat ccattgcaaa atggctccgc
    32701 cggcattttc aaaaaggtag tccgcataat tcttgatgtc cttattgccg tcaatcgtag
    32761 caaggaatgg aatcacaatt aaacgacgcc aggttccttt atcaatcgct ccaacttttg
    32821 ggaggtggtt ggtatagagc accaaagtat gcgttgggac atacttgaat gggtctttgt
    32881 atttcttctc agccgcaatt tcatccgtcg agcagagctg cttaatgtta gatgtgttga
    32941 gtcgcatccc ctcttcaagc tcggctgcga tgagtaggcg tttccccttc gcttccgcaa
    33001 gttcaggttt cacattgcgt cggatttgac ttgtaagaat atcagctgag atgctgccag
    33061 agtaattacc gagcacacgg ctgatgacat tccagaacgt ggacttcccg ttacgccctt
    33121 caccatacga gatgataagc gcttcaacgt aaactttacc aatagctgca agtcctacaa
    33181 tcatctggac ataatcaatc aactcttggt cacctacgaa aatggtgttc agagcgtcca
    33241 accaaatctg ctgattggcg tcactcgggg cacattccgt ttgcttcgtg atgtaatctt
    33301 ctgagttgtg gtcttgtgat ttgcctgttc gcaaatcaaa ggttgctgtt ggtgtgttca
    33361 gcaagaactc gttcacatct agtaaccgtt gctcgatttc aagcatgggc tttgcctcgg
    33421 ttaatgctga cgagagatac ttgctgtcgc ggcgtttgac agcaaacttc ttatactctt
    33481 gtgcgtgttg gtacaaatca aaactatgac gttgcgctgt gttgaacatg gtgactgcct
    33541 ttttcggacc aacactagca agtacatcga acgcgccatt actaaccatt tccttggtac
    33601 gcttctcaat ttcggtttca gattcttcca gttgacgttc tgtcaatgcg tggatcacac
    33661 cttgcgcttt tggtttcgac tcttcccaat agctgccgtt gtagacaagg taaccagttg
    33721 acggtgagta atgaagctta cattcatact cgcgagctaa gaccgttgct tgacctaagt
    33781 ccgagtagtc ggttggctca agtgataaat cagagttgta ctgctctgga ggaatgtagc
    33841 cttcttgttg tttcactttc ttcccaaagt tgaccgcgct attccagata gctttaagct
    33901 cctcctccgg cagtggcgga ttacattttt gggattgttc gagatacttg tgatacgtat
    33961 cttccgttgc accgtagcgt ttgatgagct ttcctgcgat gtgactcatg cttgaattac
    34021 ggctgccttc gctaatctct gactgcgcat tatcccaatc agcaaacaga tcatcttcga
    34081 gaaaatcagt gatcaactta ctaccttcat agatttcaac ttcagagttt ggtgttccga
    34141 aaagcaaacg tgccgcatcc atcgcattgt tgtcaaagta tgggaactca ctttgaatct
    34201 gcttcttgag ttccacatat tcatctttat cagttgtggc aggaatcggg aaatatacgt
    34261 gaaaacgtgg acgtgctgat ttgccacctt tgtctttcat gttgttacgt gaatagctga
    34321 ccgcaaacgt cacatcagga aactgcatcg ccacttcgaa aggggctacc caatcatctg
    34381 gattgtctga gtggtcattg tcgcaatcaa gtggaatcac atcagactgg atgaagttgt
    34441 cacctttgcg atagttgcct ttgtactcag ccatcacatg atccagcttg accgtggcaa
    34501 caagtgtttc tttatctgtc accacttgct tggttgggaa caatccattc ttggaattac
    34561 ccacctgatt ggatgtgaat aacgtcatct taagcatttg agacctcctt catctcatca
    34621 tcgaagtacc tgatatgttt cttccaatac tccgcttgtt caatttcaac tgccatgcct
    34681 tgggagatgg tgtcacccac cacccagact tcctcacatt tgcccatgag aacaagtgac
    34741 atgtgcattg ccacatcacg ctccgttgcg tcgtccatga attgtggata catcagatgt
    34801 gccgcaactg gcatgtagcc acggtcaaga gcaatacggc agtaacgctt agtgttttta
    34861 tggttgacga caacatttcc tgagaacggc gagcagacat agactgatgt tctgtaatct
    34921 ttacgtctag gcattattat cacctcgctc aatcactggt aagatgttct tcttcgattt
    34981 cagcaagtcg tacaagaaca aacgaccttt ctgcgtccag taggtatgca tcacgctgcg
    35041 ctctgcatct atcgcatgag tttttgattg tgtgtagcct tgattggcat acttctgata
    35101 aagcaaccac atcttgcctt gcttgtactg aattcccagt tcatgaagca acttgttcat
    35161 tgcctgtggt gacataccgt agtccttcga gattttagaa atcgacatca cagaattgtt
    35221 ttgaagaatc aaatcgtagt agctaacttt tggtttcatc tccaaaatca tttgttgttg
    35281 catcgccacc atttcttttt tctcttgtag ctgcttcaac actgaaatgg cccatgctgg
    35341 atcttcgatt gactttgtga tgaaatcttc tgtcgcgtac acaccgtgtt ggcggataga
    35401 cggcaagacc tcatttgtca cccaacgctt gaatttcttc gcgttcggca tcttgctaga
    35461 aatgatgagt gagtacaatc cactttcgtt gataaggata gccattgtcc cattaacgct
    35521 gaacgattcg ttcaccgtct tatcttcctc atcaacatga tctcgtaatg ctttttgtgg
    35581 atttgtgtag ccaagaattg atgctacatc tttaccaaca aagtgaggga catcgccaat
    35641 catcaccgtg cgtacagagc caaactccgc attttcaaaa actttcaatt cgttcatctt
    35701 gtgaacctcc tgtaatagaa ttttcaggag aggcattttg ccccttctaa cttacaggcg
    35761 aagaaaatgg tcgaaagtaa actctgactc gaatttattt ttgatttttt tgccttcgtc
    35821 tataagcgaa gaaagaaccc aattcgaacc ttatttttga taaaaaaatt tgaaaaaaat
    35881 atggagccat ttctgactcc acattgacta atccttttga tagaattcac actcgtatcc
    35941 atcagcgttc agtagcaagc cttcagccca cttaggcgtt tgactcatca actgacagat
    36001 gtcttcaacc ttcgattcca ttggcacttc gataatcact tcgtcgtgga cgtgcccgac
    36061 aatgcgatac ttacgaagat tctccattgc gaaacagaga atgtctctag ctattgcctg
    36121 cacgatgttt tcgacaaact tgggaccata gctttcaaga cgttcccatt tcttggtagc
    36181 accgacacct tcataggtaa cagcttcacc gccgaatttg ttctcaccaa tctttggttt
    36241 tacataaact aatttacgtc ctgacaagag ttggatgaac agaaaacccg acttgtattc
    36301 aaacttgatg ccatgcgtcg ctgtgctccg tcgttctttc acacaagtct ttactgcctt
    36361 atcaacagcc caccaaagct ccgttatgtt aatgtttgag tctcgccaag aattaacaag
    36421 cggctgtaat tcatcctcag ttagtcccat ttcaagagca tccattgctt tcaatgcacc
    36481 caccgatcct ccgtacccta gagcaagctc agcaattttc cctttttgac gtagatgccc
    36541 attcacgcca tgcttttcaa ctggcacacc gaacatctga ctggcagacg cacagtaaat
    36601 atcgccacca tttttgaata cttcttgacg ccacttctca cccgacagcc aagcaatcac
    36661 acgtgcttca atcgctgaga agtcagatac gataaatttc atcccttcac gtggtacaaa
    36721 cgctgtccgg aggagttctg agagtacgtt cgggacattg ccgtagagaa tgttaagcat
    36781 ttcaaaattg ctttgtttta ctaaactacg cgcttcagat aaatctgaca tatggttctg
    36841 aggcaagttt tgaagctgga tgccacgtcc tgcaaagcgt ccggtacggt tggctccata
    36901 gaattggaat aaaccacgag aacgattatc agaacacgca aagttttgca tggcttgata
    36961 ctttttcacg gatgacttgg cgagttgctg acgtaagacc aggacttgct ttaaattttc
    37021 atcaataggt tgttcgagta gttcggccac tgccttttta ccgagtgact cggtttcaag
    37081 tccgtggtct agtaaccatc cccgcatttg aagcacgctg tttggatttt ctagtccagt
    37141 taattcttgc aattgttgat tcaatccagc ttttgataat tcatccaact gaacggcagc
    37201 ttccacaaag tccttatcaa tctcaattcc tcggtcgttg atttcctgat ccagccagta
    37261 ttctttccaa acagaatcaa gcactggaaa attggaaagt tttccttgga tagctatttc
    37321 aacttccaca tcacgactat tgtatgtctt aaatgctgac catttagttg gcgcatggtg
    37381 tggtaagttc ctcgtgcgtc caccattaac tttggtcgga ttgcatggca cgcaaaagta
    37441 tcggatgagg tcttttcctt ctgtgagctt ttgtttatcc aaaccaagaa cagcaccaac
    37501 tcctacaagt gacaacggca aacctaatgt cgcaccccac accattgtgc attgccaccc
    37561 tcgtggattc aaaaagccat ccaaccccag aaagcgtgac aaacacacac gttcaaaact
    37621 cgcattgaag gcgtgtttaa tcactgtttc atctgatagc gaagttaata tttcagccgg
    37681 gatagtttcg tccatcttca gatcaaccac ttcgacggga ccaccatcta cactgtatgc
    37741 aaatagcagt atttcaaaat cgggggactc tgaatatttg tagacaccac atttcgataa
    37801 atcgacacta ctatacgttt ccaaatccaa tgacaaatgt ttcattttct tcctccaatc
    37861 aaacaggcga cagcacttct gccatggccg tttttgtttt aatgattaag ccaagaaatc
    37921 atcatccgcc aaggtttcaa actcatcgct tgcagatact tgtgagccga gtggttcgcc
    37981 gtcacgtaca aactggatat tcccaagccc tgccgctacc ccgcgatttc catttgcgtt
    38041 aaaggcatag aaattaatcg acacacgagc gtagcaacca ctatacactt gtgaacgatc
    38101 caagattggt tgaacgtgct gatccacgat ttgtggtgca gtggttgagt tcgcattaat
    38161 gaagtaatgg ccttggtatg cttcatcgtc gcgttcgata tccccatcac gtaatggcag
    38221 cttgatttct gctttgtttg gtttcttccc accgaatttc gcaagtccct cttcgattgc
    38281 tgcatcgatt gctttttcaa tggtatcaat cgttctctta tctgattttg gaatcaagac
    38341 tgctgttgaa tatttctctg cgcccccgtt gatggatgct ggttcccaac cgtggaagta
    38401 tgagagacgt gtgtttgtac cagtgattac tttgcttgtt ttagacatat tattgttcct
    38461 ccgttaatgg tttaaattct tcgtttaagt tgtttactgt gaccgcttgt cgtttatctg
    38521 aaccatcgac tagcgtcagc ttgccttttg gtttgatgat gtattcgccc aagatttcat
    38581 tgaatttctc tttaccgagt gccttcgtca ttgctgtgac accaagtaac tttttatcat
    38641 atgggtcgta tccagccttg atgaccgccg cagcgacatc attgtcatta gaatacttac
    38701 gagtagagcg accctcgact aatttgtgat gagtccattg tttgccaccg agtgcaagtt
    38761 tcaaagcaca gtccttgata tcgttactcc aagaaaccag gtcatccact ttaccgatga
    38821 tttcttcgat ttcatcatca ctaagaagct ctggtggttt gaactcatat tgcgccagtt
    38881 tcaagttatg gtctgctcgt gcacgacagc tattcttcgc cggacagaat ccacaccatt
    38941 tcccgcattg gtactcaccc tcacctttga aagctaaatc tgcggcaggc gacaacacat
    39001 cttttgccca ctgaagtaat tcttctttca gcagtgtgta cgtactcaca ttctcacgcc
    39061 ttggctggaa aatggtcatc ttgacttctt gaatgtcata aataacatcg aaaagctcca
    39121 acgctcccaa ggcataacac atcatctgtg ggttctgata cgcatctacc aaaattccta
    39181 gaccgtattt catgtcaata acagataggg ttccatccgc aatgacgaga cagtcgccgg
    39241 tcccaaagcc gtctgggacg taccttgaga agtcgagccg ctgttctact attacaatag
    39301 agtcaactgt tgtctgtttc gccttagcga cttcttccat gacgaaggtc gcataacttt
    39361 cagcacactg ctccatctcc tcgtcgtaga attcaagcgt agatgttggg tctgtgacgt
    39421 cattaccaat gaacttattc agtttgtact cacataaggt atgagcatca gtgccttgtt
    39481 gggcatacgg gctcgacttg ttttcaaagt gctctgacaa gcgaacactt ggcggacagt
    39541 gaatccaccg attacttgaa gatgcggata ggactgcgtg ttgtgttggc atctacaatc
    39601 cctccgcatc tgcaaccaaa gccgcgtagt tttctgtcgg cacatcactc aagcgactgg
    39661 caccatattt ctcaatcagt gctttcactt cagttgactt accttcttga gatttacgtg
    39721 ccaacaaacc acggacttct tcaagcgtcg gttgtttgac ttctgatttt gtttccattg
    39781 tcggttctgg tgtttcaact tgtggttcag gttcattggt tttcatcgcc acagccaact
    39841 cttgtaagct atcagctaac gcttggacgt cctcaacgac ctgaagaaat agttttgttt
    39901 tactcacgtc attacgctcc cttcttgtta acttcacgga tttcgactga ttcgattgtg
    39961 tcccctggtg tgagcacgag aattttgtcc atgttgccct taccgaacag ccaccgtgca
    40021 aaccagcgag gcaaatgacg cacaccacct tgtaggacag tttctttgtt acccgtgtca
    40081 tcagtcacgt ttactcggat tttgtgtttt aggttcatga tggtcctcct tcatctttat
    40141 ttgtttagga tctactccct tacacaatac aggcgaagga aaacggggaa agtaaactcc
    40201 cccgtaactt ttttctaaaa taattctttg atcttgtctt tgatgattcc aaaggtgcga
    40261 ttgattgact gttttttctt gtcaagcttg tccgccactt cagtctgaga catgttttca
    40321 gtgacgagca tgtgatagat ttcttggtgg tacggtaata attgcgtcac gacttcacgc
    40381 aaacgatcga tacgaggatc ttcttcatta tcttggtaag catcgtaaac caatgattgg
    40441 atggggttgt ccccctcatc gcattcatca tcaaattcat tgagcgacaa attgtagtta
    40501 cgtttgaagt aatcgttggc ttcaccatcc acgtattcgt ccattggata atagccgtgc
    40561 tcattattaa agtcttcacg gaacttctct ttccaagcct tgatcttttc tttttcttct
    40621 tctgtgcgtt caggacgtgc gtttttataa aagttgtaaa tcgcacggtc gtcagcgcga
    40681 tgcagctcct gaatgttaag ctcagtgacg ccgtcttcgc ctgggcgaat ctcaaccact
    40741 tctttttgga tacgtccatt ggcatcagta cgctcaaaca cgtacttgta agtgctgcgg
    40801 tcgttttggt gtgttttcat aaatctactc attagatccc tctttcttgc ctgaaagagc
    40861 ttttgagcaa ggaaaacaaa cagggaccgt gccaatacgt acaccgtccc tcatcacgaa
    40921 aaaagagcgc aataaagtaa ggcatacaat atctgaaaaa ttttctggat aaccagttct
    40981 tttcagtatt tgtaaccttg ccctattgca actcaggcat tgttatattt tattagtagg
    41041 actattcgct tttaagataa attcgttcac tttcttgaaa cataatgtag tgtttatgaa
    41101 ccataaatga taaaattaaa tagttaaact actatgtttt aattatagaa aaaggcctcc
    41161 cttgttttcg tcagtcatcg gcggcaaacg gcggtcaacg gcggacgtaa caaaggaggt
    41221 tgtattttgg aaataagtga aattactcaa acgctttatc cgtttatggg aagcgagtac
    41281 gtgaagccac aggatttttt aattgcttta ctggatagtg tttcaaatga tgacgaggcc
    41341 accattttcg gtttttctga tgataaccta cgaagatttt acaatggtac tcgaccatta
    41401 acacaagagt atgcagttcg ggttgctgga aaattcaaca tcgacaaatg cacgactttt
    41461 atttatgaac gtcttataga tgatgctcca accgtcctta agcaacaatt gattgataaa
    41521 ggtgtggtaa tccccaaaga tacagacgtt tctgaaatag tggcccatct tttacttgat
    41581 gaaatcacct caattgcttc tcaaacaaaa gtaaaaacaa aaaaggccaa gccgaagctt
    41641 agcgaattag agctcgcaag tttaagaatc aacactaatg gtgatttctc ttataatgat
    41701 gaagtgaaaa aattctttga cgatttgcca gttccccttg atattagagc tgaggagatg
    41761 acgtatgtca tagctctttt ttcagcatat gcagatgccg atggatacga agtatacgag
    41821 catacaaacg acctgccaaa ttcgcggcta aaggatttca agagacagcg aagaaattac
    41881 tataaagctg aaagcatacg tcgtggggtc cgtgataact ttacacaaga ggaaggaact
    41941 gagcactttg aagctttaaa agaagatatg tacgacggca tcgaggaagt ctatgaagaa
    42001 gactacgatg atggcgttga gagactcaaa gccgttctac agcagtcgtc tgttataaca
    42061 ttaaacggat caatcttatc gtttataccg ggattgctgc aaaattctgc taaaaaaggc
    42121 atttgccaca tgctggttaa tgacggaaag atcaaatggg tgactgaaga tgaatagttt
    42181 gtttaatact ccatttgaaa tttcggttcg agtaatgtta ctgctctctg aatttaaaga
    42241 gcctgtcagc gctgatagaa ttttaatcac agacttcatc accacgtatg gtcgcgactt
    42301 tgaaatttcc gattacaact taaatggtga taacagctat agattcagtg aatttattgc
    42361 gcgacgtgaa ttagttaaag atgcagtcaa aaatcttgta ctgcaacatg ttatccaacc
    42421 atcgcaaagc aaagaaggtt ttaagtatgc tttatcagaa gatggcctaa agttgatatc
    42481 acaattcgta tcggtatatg cagaaaaata cttacaaata gctgacgaat ctgttaaata
    42541 cattcaagat aaaagtgacg ttgagcttct gcgacaaata aaccaacgcg caaaggaatt
    42601 taaaggagag caatgataat gcaaaattat tatattgaac gtgttaccat aaaaggcaaa
    42661 gatcgtgaag atgcaactgt caagttcacc aatggactaa acataatttc tggcccttca
    42721 aataccggaa aaactagcat tgttacggca atcgattttc tctatggcgc gaaaaaagac
    42781 gctgctccct tttcaaccga tgcaaccgga tacagtaaag ttgaactgat tattcaatcg
    42841 actaatggca aatattcatt aactagagaa tttgatagca actccatttt ggtatccaaa
    42901 ataacgagtt caggtgaata tgtagattat gaaaaatata atgcacgatc tgggaaaaat
    42961 ccaattagcc atttttggtt acaactttta ggcatcgatg atgattatca gattatcaag
    43021 aatgaggatt tcaaacgtca aaaattaact tggcggacat tagcgcctag cacacttatt
    43081 ccacaagata gagtcaatac cagcgcctct gttctttttc ctggggaaac aacccaacgc
    43141 acagcttttt tatcatcgtt gttacttctg ctatacggtt cagattttgc gaactcggaa
    43201 gaaaacgaaa caaaagaaca taaaaaaatt agaaggaact cggtgattaa ctacatcaat
    43261 tcaagccttt ctgaattata tgaaaactac gaagtggcta aatcagaatt acctgaggct
    43321 ccctctgatg attttcaaca aattgtcgat ggaatgttcg ctgaacttga aacaattgaa
    43381 aagcaaatca atgatgcgtt agccagcagc aaaatcttga taaaagagat ttatcaaaag
    43441 gaacagtatc ttcatgaaac tcaacttctt ttgaataggt acaaacagtt acattatcaa
    43501 tatcaatcgg atattgaacg actaacacta atcgtagatg gtgagcgatt attaaatgat
    43561 cataatgaac atgatacttc acatagctgt ccattctgta acggcgaact aagtaaacct
    43621 gtccaaacaa cctacatcga tactgctagg tcagaattac attcattgct ctctcaatta
    43681 tcagatttga cggaagaatc tcatgatacc gaagttgaac tgcaagagtt aatggaacag
    43741 cttgatgagc ttaaaacaag aaagaaatcg cttgatgcag caatcaacga tgaactaaaa
    43801 ccgaaagctg agacgttaaa ggatcgaatc aataaaatcg aaacttacag ggatattaaa
    43861 caacagctaa tctcgatgga aaaaatgcaa aataagctta aaagtgatct tgaaaaaatc
    43921 gagaatgagc cagaagacga acgtatcctt tataaaccta aagagaaatt tgatgcagaa
    43981 ttctacaaag aaatgtctga gttactggac tccatgttac gtaatatgca ttataaacct
    44041 aatgaatttc gtaacgcatt tttcgccaag gatattttcg acatcaaaat caatggaaat
    44101 ctaaaatgga ccgaaaacgg acatggatat acagcgttta tcaattcagt agtggtaatg
    44161 gcatatcgac atctcttgta taatcacgct aagtacacac cttctgtgtt cattatcgat
    44221 tcccctcttc ttggattaga tgaacgcaaa aaacaagatg gcgatgacaa tatgaaagaa
    44281 ggacttttca aatactttat ggatcatcaa aacgaaggac aacttatcat cgttgagaat
    44341 acaacgaact tgccagcact aaactatgat gataaacaag taaatcagat tgtctttact
    44401 gaagataaaa acaatggacg ctatggatat ctatacgata tttataacta gaacaggaat
    44461 gattacatga agtttagcta caatccgctt tggaaactcc taatcgacag aggactgaat
    44521 aaagcacaac tcagaaaaat gactgatatc agttcagcaa cactctcaaa actaaataaa
    44581 ggcgaaaatg taacaacaga tatcttactt cgcatctgtg aagcacttga ttgtgattta
    44641 tcggatattg ttgaaatcgt tcgcgattaa atatcaaaaa tgcactgtat tcgattcatg
    44701 agtacagtgc attttcatat ttatttttgt aatccagcga ttacttcatt gattttcgat
    44761 tcaatatcat tcaatggaac catataccat tcagtagctt ttttgaagtt tccatcctga
    44821 aagacaactt gtttgtcctg gaatacatga tggagagtgc gctcaagtag ctgagcgttg
    44881 acattttgta cttcagcaga aagcatgacc ttcactggtg catttaagta ggtcacgtcg
    44941 ttttccgcat tggcaattct actttcaaca tcatttcgag tgaaaccaat cttatacagg
    45001 ttattccact tgctaatatc cggattcgtg cttaatgact tcagcacgta gatataacct
    45061 gtcgtaaact cttctccaaa gctttcagcc agtgtttcct catctaccgg ctctgtcacc
    45121 atccttccat gacgttcagt cgagtataga gacgaagcta aagattgtaa caacagattc
    45181 ttattctctg tcccattctc ataaacaaca tgcattctgg catttgtttt cccattatca
    45241 gcgataaagg tatccttcga aatggaaacg atatacatca ttacgccgtt ttggatgtag
    45301 aatctatttt catgaatatc atactgctca aacgggataa tttttcgtct gccagatgcg
    45361 atatctgcat gcacttgatc gaacaagctc ttatactttt caaaatgact tgcatgtttt
    45421 ctgcggccaa ttttatctgc agcgttcaca gttctacgat agcgggaaac atccaacaag
    45481 tctgaaaaat catcaatcat atcatctgag cctaagatat catctaaatt caactcgtca
    45541 tcgatttcaa ctgaatcagc gagaaactct ccatccggtt cgaattcttt ctccaataaa
    45601 ccaagagtgt catacttctt aaccttctca gacctttcgg gttttcctcg aaatccgcta
    45661 agacgagccc acaatgcgcg ttcactagac cattcaggag ttttttctgg ctctcgtccg
    45721 ttttcattca caaagtctat aatctcctga aatttagcaa cttctgggtc ttgttttaca
    45781 actttctttt tttcaacttg cttggttaaa tcatcaaaag cgtcatcttc aaagatgcta
    45841 tctaaactat tcgccatctt ctttcgctgc ctctctttca cgttttatct cagcaagtcg
    45901 tagcaaagca aaggccaagt atttttcacc atcatcttta ctatttcgat caggatttcg
    45961 accattttct gcacggaact tcttagcagc ttggtaggcc gcaaccaatt gttcgtcagt
    46021 aaaatcatac ttatttgatg acaaataatc ctgaataaac cgaagagtcg gtgcatcaat
    46081 atcacgacta atcacttcat acgcacgttg gaacggattc acttgatcga ttaaatcaat
    46141 attcaattca tcaatgttaa tgaaacgatc ggccatctta ataatttcgc ggctaccatc
    46201 ttctgaagtt gttgtccgtc cagccgcaat atttatatcg gcaactgtat gttgacgaac
    46261 ggtttctaac tcttcgttcg tcaaatcagg ataacgatca atgatgacac gtggaatcaa
    46321 cgtcttatta atcatcttcg catcagcacc tgaaacaatc gcattttgaa tatcttggct
    46381 ctgtaaaatg ctagatttca aatcttccat atcattttcg atgattttct ttgtacgttc
    46441 cgtcgtatca gcaggtttca gtcctctgat tgaaagatca cctggtttgt ctggcttacc
    46501 atcttttggt ttaaacttgt aaactggcgc caacacttgt tccataagta gagatgcaga
    46561 aattgccttc ataatactgt taaccgcgaa taatacttcg tcatcctgtg catctggctg
    46621 cgcaataaga tttgtaaact gtgcatgagt cttattgggg ctgtcacgag ttacgcgacc
    46681 aatgatttga atcacttcag ttaatgagtt acgataaccg attgtcagtg catattccgc
    46741 aaatggccaa tcgaagcctt cttttgccat tccaagtgca atgatgatat caagcttatc
    46801 aagatcttta atgtcatccg taatacttgc tagatatgct gagacttttt cacgtccttc
    46861 ttcattcaca aggtcggcca ctaatagttt tttaccatct ggacgttcga tttcataaat
    46921 accatcctcg ttctgtgtga tattaccacc gtccgtcaat aaatcaagta tttcatcaac
    46981 ttccgcgtat ttatctttag tagactcacc agaattaaca ttaggaatat ggataattga
    47041 ttttttgttg acatcaatta ctttatccaa agcatctgta tattttccac gatagaaact
    47101 ataccccatt gcaaaggatt tcagatattt atatccttcc agttgctcat aataagtgta
    47161 agtgacctta tcaaatttat cttcgtcggc tggttccaga atctgaacag agtcgccacg
    47221 gaaatatgaa ccagtcattg ctaagatatg cgcattagag tttgacatga tgctacgtaa
    47281 ggcattacct aacacagatt tatcatcacg gctcacatga tggaactcat caatcgctaa
    47341 tagcgtattg tcaaataatg aatcgtccaa ctcttcaaaa gcaaatcgca acgtagcatg
    47401 tgtggcaatc aggatattat cacttgggtc agtcgagttc atgaatttaa caaagtgttg
    47461 aacctttgat ttttcaccgc ccccaatagt taagttgtca cttggcttta cttcccaatc
    47521 ccaataaaaa ccaaacttcg ataattcagt atctttaaat gatttaccaa ttgagcgttc
    47581 aggaactgca ataaccactt ttttaattcc ttgattatgg agtttatcca agccaacaaa
    47641 catcaatgca cgtgattttc ctgaagctgg tggtgatttt accaacaaat actgtgacgc
    47701 acgcttttca taaacacgtt cttgcatttc acgcataccg aggtcatttg ttgtttttga
    47761 ttgattttta tcaaaattaa tttcaaataa attactagtc attgattgtc atctcctgat
    47821 agagttttag taatgttcca agtcgatctt cgtcagattc aaacgggcgt tgctgataag
    47881 ctctatcaac aatcccatcc agttcctggt gtttcttacg taatgattct ggcatactgt
    47941 ctttattata caaatcggct aacgtgccgc cttcgtattc tcgtaaatca agaatttctg
    48001 tcatcacgcg agtcatttca tttttacgct gggcggataa atcctgaatc ggaaaagtat
    48061 tatagactaa cccggctgaa taacggtacc gagtttctaa tttcccaccg acagagcgca
    48121 gccacaccat atgcattcgg gattctaaca gaccaagcag ccacattgga gcgttataga
    48181 ttgccatagc cgaatctgaa attatagtat cgctcccaac tatacccata ggaacataat
    48241 ctcttttttc tgatgacaca cgaggaatta gaatactatt ctcactggtt tcatcataac
    48301 gaacttcacc aaatgaccat ggcttctcag ctagtgctcg agtttctttt ctcttgcttt
    48361 tttccctata actttttaca ttttttattg tttctttaat aagagaattt tgtttgtatc
    48421 tagttgcatc ttcctcggtc aaccataaag tatatcttaa gtctccattt attaatcctg
    48481 ttgatcctaa atatttctta aataaatgtt taatatctgg atatttttga atcaaattcc
    48541 agtattctgc cactgaaaaa actagattac caccatcatt tgccatactg ccaaatttca
    48601 aagagggtaa atttgaaata ctatttcttg attcgttgac aaatacattt tcaccattag
    48661 ctaaatatgc attaatattt gaaacactca ttactgaccc attctctaaa tataacttag
    48721 gatgaatatt cacattattt gtcataccaa taatgacaac agtcacaccg gcattattct
    48781 ttgcattgtt gttccactta aagctctgat atgcaaatga aattcttacc tcatctttta
    48841 aaaggtacgg ccaaaggata ctaacttgtt caccttgaca aattgaatta gtagtaacaa
    48901 atgcgaacga gccatgagat gttgcaatat attgtttagc tttccaaaac cacccaaaaa
    48961 tataatcaag caatttatat tttcgaatat cgccaacaag tttttccata tcttcacgtt
    49021 gttcagcatt ttgaagactt ttgcccagat acggtggatt tcccattata tatatttcat
    49081 gattttcttt gagtcgcaac acctcattcc aatcaattcg aagagaattg gcacacacta
    49141 tattcccagc atcttttaac ggtaacaact cagcaatggt acctggtatt gcttcttcaa
    49201 gctctttatt catttgatgt tcagcaatcc ataatgaaag tcttgctact tcatgcgcaa
    49261 aatcatccaa ttcaatccca ttaaaattac caagatgaat cactgatagt ggcatggaat
    49321 cattattttg taagtcgccc aacaattgca aaatcttaat ttcaagtcta cggatttcct
    49381 tataagcaat gatcagaaaa ttaccagaac cactggcggg atccaaaaac ttaatattag
    49441 aaattcgcgt caacaaagca cttagatcat tgatgattgt tctcttatta tcgtttcttg
    49501 ttttttcagt aatgtccttc agctcgtttt cttcactgcg aagctttaaa gtttcaaatg
    49561 actctgtcaa atcatctaag aataacggct tgatgacttt catgatattc ggcacgcttg
    49621 tataatgcat accggcaacg tgacggtctt ctgctgaagc aactgattga atcattgcgc
    49681 caaggatatc aggattgatt tcattccaat tcaataattc ccctgcttca ataagcagct
    49741 ctcttgaaac ttttgaaaag gtcaaatcca catgtggctc accaaacagt tttccattaa
    49801 catatggaaa ttcaagtagc cactcatcct tgccattgcg agctaactca ttaatatcca
    49861 agattgcaaa gagttctttg attacttcgt taaaattcga accatcttca gccgttcttg
    49921 tttttagaac atttgtaaag gaacccttat tcataattcc tgtatcttct gcaaacaaca
    49981 agaacagcac tcgaatcaag aacaagttaa atgcatgctc gttggcatca ggattatctt
    50041 tagccaccac atcatacaat tttgcaaaac gttcagcagc ttttcggtca gctggatttt
    50101 cagcttgata atcagccttc tcaatgccat tccacgctaa gaaaaagtca gcattagccg
    50161 gtagctcttc aattggaata ttcaaagttt catgagtttt cgtatcaatt gcagcaatat
    50221 ctaaataatc gtttaccaag atatatcgtg gtttagattt ttgctctttg attgattgag
    50281 caatcgcatc aatcgcaatg acaacgttgc cttcaatttc ttcataatga agtttgttct
    50341 taatcacaaa tggttcacca ttatcgaatt ttgattttgc acgtgtaatc gacgttttgg
    50401 ggatatcgta caaggacaaa aattgagtga tgaaatcatc gcccctattt gaaagtattt
    50461 tttttacacc atcttcaatt tcagtgatgt ttaaaacttt attttttgcc atttatttaa
    50521 catcctccgc atctataaca tctgaactaa ttatttcact aacatcaaca tttaaggcag
    50581 cagcaatttt caataatgac tccattgcaa ccaactcatt tttaccgagt tttgctatta
    50641 cattgggact gacgccagat atttcacgaa gttgagtttt gttcatattc aaatcaatca
    50701 ataacttcca caatttatta taagaaattg acatactaca cctctattct actggtatct
    50761 tcaattatat catctatgag ctatcattat tatacgaaac atgaaatatt ttcgcgaacg
    50821 caaactattt ttctaaaata aaagccttca gcatagattt actaaaggcg agataacaaa
    50881 tattaattcc actttatttt aaccggaatc cccaattgac gagctagttt atcaattttg
    50941 attctgacat tctttttgtt gaaatttgca taaacccata ctccatcagc taccctggct
    51001 gaatttggtc ttccgttata catggatggt ttacgtccta aaattatagc gaacgacggc
    51061 gtctcatcga caattaattc ttgcagttga ttgttgtagt ttccatccgc aaatagctga
    51121 gaaacaaaat caaccaataa acctctagca gatttcgaag atatcttttt cccatcatac
    51181 tcaatttcat attgtccaga tgaactaaat tcataaattt gggtttcact gtcttcggaa
    51241 gtaccaaaca aatcgatatt tgcaatttca tctataattt caaacactgt gtcatatagc
    51301 tgatccgatt ttgctttttg aagtttatca atatacgaag tattaccagc agttgaattc
    51361 atcatttcat agtcagattt ttcttggaaa tcttggataa gtcgtttttc aagataatct
    51421 gtatctgctt ttgacatttt cccatctgct cttgtaaaga ataaaactga ttcaacccaa
    51481 gctttatatt ccttgagaaa gtgttgagat aaacgaagtg aaattgattg ccctgctgcc
    51541 tgacccacgt aacgcttatt accaccaatc aaaacataaa cagctggata atttgaaaaa
    51601 tcctgttcct tcaaatacgg caaatcacta cgactcattg aaaccaagat tgctggctcg
    51661 cgttcaaaat aagaaatatt atctttctgt gtatctgaaa atataaaaat ttgcttcata
    51721 agtttgtctc ccttgaaaac tcgataatat attatatcac gtaaccgtta tcaaaatcat
    51781 ctaacctatc aagtctacgg tgtaactcct atcaactcaa agctccatag catttccatc
    51841 aactctacgc tcacccaaaa atcgtcattt caacacgaaa aaagagcaat ccggaaacca
    51901 tcccgaatcg ctctaatcct tgtcatatca acgttccaca cgtgtaagca gtcctacaca
    51961 ctcgatgtga tgggtttgcg ggaaaagatc tacaggctga actttcttca cggcataacc
    52021 tgcttctgca aataacttag catctcttgc aaaagttgcc ggattacatg agatatagac
    52081 gattcgttct ggtgacattt cgattgctga ttcaataaaa tgatgatcta atccttttcg
    52141 tggagggtca actacgatca cagatggttt gatccctttt tcttgccatt ttttcataac
    52201 tttttctgct tttccgactt cataatgaac attttcgatt ttattattta aagcattgaa
    52261 ctgtgcatct tcaattgctt ctggaataac ttccatccca tacaccttat ccactctgtc
    52321 tgctaaagac aagccaatcg tacctattcc tgaataagcg tcgatcacaa tgtctttttt
    52381 atctaactcg gcaaactcga tggctgtttg gtacagatac tctgcttgtt gagtatttac
    52441 ttgataaaat gactttgctg agattcgata ggtttttcct aatagctgat cttcgatata
    52501 gtctctgcca tataaaactt tttctttgtc tcctaaaatc acatttgttt tttcttcatt
    52561 gatattttgg atgaccgaaa ccacttctgg caattcttca tgaatagctt cagcaatttc
    52621 cttggattta aagaattttt ctttacgagt aactaaaacg accatcattt catgggaata
    52681 atgaccacga cggataatga tgtgacgaag ctgtccttca tttttttctt cattatatcc
    52741 tcgtacttgg aaccgttgca agatatctct gactactttg attgcttgat cgatgacagg
    52801 atcttggata taaaaatctt cgatagggac tagatcatgg ctattctttt tgtaaaaacc
    52861 ggtggctaac tgtccatcga tacggcgaac agggatctgt gctttattac ggtagccgaa
    52921 tggttctgcc attccgaccg ttggaagtac aggaacttct ggcatcttag caatcttcgc
    52981 cattacatgc tctacttgtt tttgtttgaa gacaagctgt tgttcatact tcaaatgggc
    53041 taaaggtgcg atacctatgc gaagtaatgc attgttttct acaggctgac gatcaggtga
    53101 ttgtgtaata aatgtttcga cttttccata tccaaactta ttgcctgctt ttactactag
    53161 tatttctact tcttctccag ggagtgcatt ttcgataaat aatggataac catcgatttt
    53221 tgctaccccc attccttcat aactcaagtc gacgatttcg actgtatatc gttcgttttt
    53281 ctttactatt tgctgttctg tcatattttg ctcctttaag cctatttttc aatcctactg
    53341 aactctacta taaatcagct aattacacaa ctaaaaccgg cactgacctt actacgttca
    53401 gcaccggctt ttgattaatt cttttgatta ggtgtttcgt ctgctatttt tccgtcaccg
    53461 tcgatatctt catccgttaa acgttcgact tcttttacga attctttgct gacaacctct
    53521 aattcttctt catctgtacc tgtgattgct tcatcaggaa tctgatcggt attggcaaag
    53581 aactcaatgt gttgatgaag attttggaaa tgcatcggtg cttccccgcc gtattcacca
    53641 tctaagttga tcattactgg tcgattgata tcatcttctg tttcaacgaa caaactgctt
    53701 gttttggtat agattattct tggatcatcg acatgtcgac cgccattcag catgagtgct
    53761 gcaatatgca agatctcaaa gatatttgct gtcttcacaa tgattaaaga aaacttccca
    53821 tcatccaatt tggcatctgg cgcgatctgt tcgaatcctc caacagagtt cgtcagccct
    53881 aggaaaaaca tcgaagcatt tccgatatat tccccttcat catacttcaa gcgcattttg
    53941 attggcttta ctcgagggag catctctgct cctttcgcaa gataagcaag ataaccaaaa
    54001 atactcttta gttctgatgg aacttcataa gtcaattctg tcaaatagcc acctgcagcg
    54061 atattgatga agtaactgtt atcagaaagg ccgatgtcca tttttacggt ttggtgtttt
    54121 tttatcactt ctgctgctgc tttaatatta tctctaggaa tttttaatgc tcgagcatag
    54181 tcgttcgttg taccagctgg gatgatggcc attttaggac ggtgttcaag cggagcgatt
    54241 ccattaacca cttcgttgat tgtgccatcg ccaccagctg ccactactag atcaaaacct
    54301 agttctgcga ctctacatgc ttcattcttg gctgaatctg gttctggtgt agttgcatag
    54361 gcgctggctt caaatcctgc ttcttctaat gcctgtagga tatccgccaa attttttttc
    54421 aataattctt ttcccgaggt ggggttataa attacgcgtg cttttttcat tttcagcctc
    54481 tatcgtttcg ctaattcatc tttaagtagc ttattcacga caccaggatt tgcttgtcct
    54541 ttagttgctt tcatgatttg accgactagg aagccaaccg cacgatcttt tccatttttg
    54601 aaatcatcga cagattgttg gttgttatcc aaaatttcat taatgattgg cagtagtttg
    54661 ttcggatcag ataattgcac ccaaccattt ttttcaacta cttcttgggc ttctccgcca
    54721 tttgtgatca attcacggaa gacttttttg gcgatttttg agctgatcgt tccatcggcg
    54781 atcaaacgaa tcattcctgc taagttctca ggtgtcaatt ttgtctcagc taattccagt
    54841 ttctcactgt tcaagtaagc agaaacttca cccatcaacc agttagatgc ttgcttggca
    54901 tctgcaccat tttccaaaac agcttcaaaa aaatcagaca tttcttttgt aagtgtcaat
    54961 accattgcgt catactctgg aagtccaaac tcacggatat agcgttcgcg acgagaagct
    55021 ggcatctcag gcaaactagc ttttactttt tgaatccatt cgtcatcgat cacgaatttc
    55081 gggatatcag gctctgggaa gtaacgataa tcactagacc cttctttgac acgcatcaaa
    55141 actgttttat tcgttgtttc gtcaaaacgg cgtgtttctt gttggatttc tcctcctgaa
    55201 agaagaactt ttgcttgacg tttttcttca aaagctaaac cttttttcac gaaagataaa
    55261 gagttcaagt ttttcaattc tgctttcgta ccgaactctt cttgtccata agggcgaaga
    55321 gaaatattgg catcacagcg catagagcct tcttccattt tcacatcgct gacttctgta
    55381 aattggataa ttgatcgcaa tgcttctaga taagcgtatg cttcttctgg tgaacgcata
    55441 tcagcttcag aaacgatttc gatcaaaggc gtgccttgtc ggttcaaatc aacgtaagaa
    55501 taaccgccaa taccatgcat gttttttcca gcatcttctt ctaaatggac acgttcgatc
    55561 cggatttttt tctttttacc ttctacttcg atttcgatcc atccgtcatg accgattggt
    55621 tgatcaaatt gggaaatttg atatgctttt ggattatctg ggtaaaagta atttttccgg
    55681 tcaaaatgag tatctttaga gatttcacag tttaatgcca aagctgcttt catcccgaat
    55741 tccagcgcag ctttattcat tactggcagc acgcctggat aaccccagtc gatcacgttt
    55801 gtattgctgt ttggttctgc accaaaatga gctggcgctg gagagaaaat ttttgagttg
    55861 gtttttaact cgacgtggac ttctagtcca atgactgttt caaaattcat tcgtctttcc
    55921 ctcctaaaat agctggttgt tgtttatgga attctgttgc ttgttcaaac gcatatgccg
    55981 cttgatacat cgttttttca tcaaaataat taccgatgat ctgcaatcca actggtaatc
    56041 cttctgataa acctgccggt acagacatac ctggaagtcc agccaagttg acagggatcg
    56101 ttaagatgtc actcatgtac attgtgattg gatcgttaat gttctcaccg attccgaatg
    56161 caactgttgg tgaagatggc ccgatgatca agtcataatc tgcaaagact ttttcaaaat
    56221 ctcgtttgat caatgtacgg acttgtcccg cttttttgaa gtaagcgtca taataaccag
    56281 cacttaagga gaatgtacct aacatgatac ggcgttttac ttcttcaccg aatccttctg
    56341 aacgtgagtt gacatataca tcatctaatg atttcacatt ttctgagcga taaccgtagc
    56401 ggataccatc gaatcgttgc aagttggaac ttgcttctga tgaggcaatg atataataaa
    56461 cagccacacc gtattttgaa tgaggcaagc tgacttcttc cacaacagca cctaatgaac
    56521 ggaatgtttc tgctgctttc aatacagctt cttttacgcc aggggccaca ccttcgccta
    56581 agtattcttt aggtaaagca atcttcattc ctttgatgtc gcctgtcagt ccctctgcaa
    56641 aatcaggaac tgaaacgcct gaagaagtac cgtctttttc gtcataaccg ctgatagctg
    56701 tcaatgctaa tgcattatct ttcaccgtac gggtcattgg tccgatttgg tcaagagatg
    56761 aagcaaaggc aatcaaacca aaacgtgaga cacggccata agtaggtttc atccctacga
    56821 tgccgttaaa agctgctggt tggcggatac ttcccccagt atcactacct aaagaaactg
    56881 gaatttgtcc agctgcaact gctgctgcag aaccaccaga agagccacca ggtacttttg
    56941 ttgggtccca agcatttttt gttttcttga aataagaagt ttctgtactt ccacccatag
    57001 caaattcatc catgtttagc ttaccaacag ggatcatatc tgcttgatag attttatcca
    57061 tgaccgttgc atcataaatt ggttcaaaat tgtaaaggat cttagatgca gcggttgtca
    57121 agatatcttt tgttacgata ttatccttga taccaattgg aataccagca aggacgttgc
    57181 tttcatcaat ccctttttca tctaaagctt ttgcctgttc caatgctttt tcttcgttca
    57241 atgtaatgaa tgcttcgacc tcaggctctg tttctttgat tcgttcaaat gttgcttttg
    57301 tcaaatcttg tacagtgatt tctttcgata caagcatatg atgcagttct tctagagaac
    57361 gattgtgtaa ttctgtcatt atgcgccagc ctccccatta tcgatgattg cgggtacttt
    57421 gataaagccg tcttctgttt ccggcacatt tttcaataat tcatcgcggt tccatccagc
    57481 ttgcgcccga tcttgtcgca tcacgtttac tgtttcaacc acattcgacg taaatggaac
    57541 accttctgta tctacttctt ccagcatttc aaccatatcg atgattttac ctaattggtc
    57601 ggtaaaactt gcaagttctt catctgcgaa cgcaagtttt gctaatttgg caacgtgttt
    57661 cacttgttct tcactaatcg ccatactacc ccttctttct aaacgagtta aaaactttgc
    57721 tctctatttt aacacaagag tgccgtgtct gaaattgttt attgaaattt gtctgaaaaa
    57781 actcattttt cattcttcta ctgagcgact tttagccaat gcatctgcca tttgatcttc
    57841 tgtccaaact tcgattccta attcttgtgc tttggtcagc ttgctgccag catcttctcc
    57901 tgctaccacg atatccgttt ttttcgatac gctaccagtc actttcccgc caagattttc
    57961 gatcgtctct tttgcttctt cacgagtaaa tcgtgttagt ttaccagtaa ggactactgt
    58021 tttttcttta aaaggagact cgacttcttg caattgggca tttcgaagcc ctttgtattc
    58081 gaaattgacg cctgcttgtt ttaattcatt catcaattca tggacttctt cgttactgaa
    58141 ataagtgact acactgtcgg caatgatatc accgatcgta tcaagcgcaa taatcgactc
    58201 atagtcactt ttactcaatg tttctagatc accgaaatgt tctgctaaaa tttttgctgc
    58261 tttcgctccg acatgtcgga ttcctaaccc gaaaatcaac cgctcgaccg aattatcttt
    58321 gctgttgtcg attgctgtca aaatattatt tgctgatttt tcttttattt tgtccaacgt
    58381 caataactct tcttctgtca atttgtaaag atcagcaaca tctgctacta gttttttatc
    58441 atacatctgt tctagtacgc gcggccccaa cccatcgata ttcatcgcat ttcttgaaac
    58501 aaagtggttc aaaccttctt tcatttgagc agggcattta ggattgatgc accgtaaggc
    58561 tacttcttca tccaaatgga caagttcact tccacaaact ggacaatgtg taggcaattg
    58621 gtattcttcg ctgtctttag gtcgtttatc taacacgact tgcgaaactt ctgggatgat
    58681 gtctcccgct ttataaatca gaactgtatc tttcaagcgg atatccttca acttaatata
    58741 atcaccgtta tgcaggctgg cacggcttac tgtcgttcct gctactcgga ctggtgtcat
    58801 gatagctgtt ggcgttacta cccctgttcg accgactgtc cattcgatgt tttcgattag
    58861 cgtttcaact tcttctggcg gaaacttgta agctgctgcc catctcggtg ctttgacagt
    58921 aaaaccaagt tgatcttgta gcgagaattc attcactttg atgacgattc catcgatttc
    58981 atagggcaaa tccacacgtt tgtcatgata ttcttcaatg tatgaccaca cttcatcaat
    59041 cgaatgacat agacgttttt cccgattcgt gtggaatcca attttttcta gctcttctag
    59101 cgcatcatac tgggtttttg cttgcatagg accgaaatca gctactgtgt ataaaaaagt
    59161 gtccagattt cttttcgctg tgattttaga atctaactga cgcaaagaac cagctgcggc
    59221 gttccgagga ttcgcaaaga tatcttttcc ctctgcttct ctgtcttggt tcaattggac
    59281 aaatgaccgt tttggcatga aacattcgcc tcttacttcg atgttcattg gttcttttaa
    59341 tttgattggg accgaccgga cagtttttag gttttcagtg atgttttctc ctacagttcc
    59401 atccccgcga gtcgcacctc tcacgaaatt tccatcttca taacgcaaag aaacggaaag
    59461 tccatctatt tttagttcac agcaatagtc aactacacgt cccactgctt tttgtacacg
    59521 ttgatcaaaa gctatcagct cttctttgct gaaaacgtcg ttcaaactgt aaagcggaat
    59581 atcatgagtg actttttcaa aaccttctaa tacttttccg cctactcgtt gagtgggaga
    59641 atcagacgtg atcaaatcag gatattgttc ctcaattgct actaattctg cgtattcttt
    59701 atcataaaca tagtcttcca ctgttggttt atcttctacg tagtattcgc gagaccattg
    59761 gttcagtctt gtacgaagtt cttctgctcg tttagcagct tcgtcaatcg tcatttcatg
    59821 ttccatcatt taccctccac tactaagctt tttcaattgg cgcaaaggca gccaaaagac
    59881 gttttactcc ttgacttggg aaagccacat ctagttctag atccttcgac gtcccgctta
    59941 ctcggacgac tgttcctaga ccccattttt tgtgtttgac tttatctcca ggtttccagc
    60001 tgttattttc ccctccagta gcacttttag agggaacaac cgctgtctct ggctgtttat
    60061 agctatatgc tgttttttta gcgaatacag gtgttttttt aggcgtagga cgcatgcctt
    60121 cgatttccag taactcctga tcgatttctt ctacaaaacg agaaggtcgg ttatattgcg
    60181 tccgtccata taatgttctg gagaaagcat ttgttaaata caacgcttct tctgctcgcg
    60241 tgatccccac atatgcgaga cggcgttctt cttccagttc gctttcttcc atcaatgctc
    60301 gagataaagg aaaaaccccc tcttctagac caatcaggaa tacaactggg aattctaatc
    60361 ctttggctgc atgcaaagtc atcaatgtca cttgggaagc atcttcttct aaattgtcaa
    60421 tatcggaaac aagtgccaga tcatttaaga agaccgttag tttttcttct ggtgcatcag
    60481 cgtcttcttc attttgctgt tcgaattgtt tgtcgaattc ttgtgtgacc gttaaaaatt
    60541 cctccaagtt ttcgatgcgt gcttgtgctt ctaatgtatt ttggattttt aagtcttcta
    60601 gatatccgct acgatccaaa acctctttcg tcaattctgt cactgtcaag tatgggatca
    60661 tctgagtgac ttcttggatc atctctccaa atgcacctaa ttgctgtcct gcttttccac
    60721 cgatatttgc cagatccaca ttttgcgctg cttcaagcag tggccattca tgaagagaag
    60781 caaaactgcg tagtttttcg atagatcctg gtccgatgcc tcgtttgggc gaattaacga
    60841 tacgctcaaa actgattgaa tcttgaggat ttgccagcac attcaaatac gctaaaatat
    60901 ctttgatttc tttacgatcg tagaacttgt gccctccaac cattttataa ggaatatttg
    60961 ctttcaacag agtttcttcc attacacgag attgtgcgtt cgtccggtac aagatggcaa
    61021 agtcgccgta attccgatga ttgcttcgaa tctcttcttg catccgatct acgataaaac
    61081 gggtttcgtc tcgttcatta tctgcacggt agtaagtgat cttgtttcct tctttgtttt
    61141 ctgtccacaa gttttttggt tttcgattgc tattgttttc aatgacttgg tttgctgcgc
    61201 taaggatgtt tttcgtggag cgatagtttt gttctagcaa aattactgca gcatctggat
    61261 aatctttttc aaaatccaag atgttctgca tatctgcacc gcgccagccg taaatacttt
    61321 gatctgcatc tcccacgacg cacaggttcc taaatctacc tgccaataaa ttgactagcg
    61381 tatactgtgc gtgatttgta tcttggtact catctacatg aatataatgg aatttgtttt
    61441 gataatatgt gagtgaatca ggatgttctt caaataatcg aatcgtattc atgatcaggt
    61501 cgtcaaagtc catacattga ttatattgaa gctctttttg gtaagcagca taacattttg
    61561 ccgcaatctc ttcgtaataa ctgccttgca tttttgcaaa atcctgaggg gtctgcaggc
    61621 tgttttttgc ttgagagatt gttcctaata tacttcgagg atcatatttt ttcggatcaa
    61681 tgttcagttc ttttagtatc cgtttcatca aggtcagctg ttcagaagaa tcgatgattg
    61741 taaagttccg gttatacccg ataaaatcaa catctcttct taaaatacgc acacacatcg
    61801 agtgaaaagt cgatacccag acatcttcgc ctccagatgc gagaatcgca ttgacacgtt
    61861 ctttcatttc ttttgcagcc ttattggtaa aagtaatcgc taggatgttc caaggattga
    61921 cttctttttc ctcaatcaaa taggcaatgc ggtgtgtcag cactctcgtc tttccacttc
    61981 ctgcacctgc cattagcagt aatggtccgt cagtgtgaag aacagcttct ttctgacggg
    62041 ggttcatccc gtttaataat tcagctttgg atgacattaa caacatcctt tcttttatca
    62101 atcttgttaa ttatagcatt ttcttggctt tcattaaact agctagttca aaatcatttg
    62161 tttcttttta tggatttttc cttgtcaatt cgttgatttc gcgctaaatt aaaagaaagc
    62221 gaggcgtttg aatggaaaaa aaaggaaaaa tcaagaaatt agataagact cagatatatt
    62281 ttattctaaa aggaatattt gttggactgc tgacaggaat cgtcgttagc ctgtttcgat
    62341 tatctatcga aaagctaagc gatttagttc gaagtatcta tgaaatgagt cgggaacagc
    62401 cgatttactt aattggccta gctatttgct gcatccttgc agcatttttc gtgggttatc
    62461 tggtcaaaaa tgaaccggat atcaagggaa gcgggattcc acaagtagag ggacagcttc
    62521 gcggggaatt atcaatgaac tggttctctg ttttatggaa aaagttcatt ggcggtgtac
    62581 tttcagttgg tgcgggcctt tttctaggta gagaaggacc ttctatacag cttggcgcat
    62641 ccattggaca aggagctgga caattgtttc ggagtccatc ttcagaagaa aagattttaa
    62701 tttctagcgg tgcaagtagc ggacttgcag cagcattcaa tgctcctatc gcaggattac
    62761 tctttgtttt agaagaaatc catcactcct tttctccact tgtttggctg acatcatttg
    62821 cttcagcaat cactgctaac ttcgtctctc tttacttttt cggcttgaga cctgttttat
    62881 atctaggtga ccttccttct ttgccgttga aatattatgg ttccttagtg atcttaggag
    62941 tacttctagg catcttaggt tttatttatc agaaggtctt gctggctttg ccaaaatttt
    63001 acaaccgatt gcctttacca gcttattttc acgggttcgt tcctttcttg ctgattttgc
    63061 caattggtta tttatggcct agtactctag gcggaggcaa tcaaatcgtt ttagcttttg
    63121 gacatgcttc cttgccaatc attatgatta tcggtttgtt tgttcttcga ttcgtttttt
    63181 ccatgatttc ttacggtggt ggtcttccag gggggatttt ccttccgata ttgacattgg
    63241 gggctttaat cggtagcttt tatggaaaca ttatggttga tttcgggatg gatccaatct
    63301 acatcaagga ttttgtcgtt attgccatgg ctggttattt cacagcgatt ggaaaagcac
    63361 cgctgacagc aattatttta gtgacagaaa tggttggctc tcttaatcat ctgatgcctt
    63421 taggattggc tgctcttgtt gcttatatcg tcaatgacct tctcggcggt aatccaattt
    63481 acgaatcatt attggaacgt ctacttggaa atactcagac cgcctcacta aaaggtgcaa
    63541 ggacaacttt tgattttcca gtaacggcag agagttcttt agacggatta atggtacgag
    63601 acttcaattg gcctaaaaat atgctgctaa tctctatacg cagaggaaat caggaaatct
    63661 tgactcacgg tgatacgatc atgaatgtag gagatatttt ggttatcttg actgatgaag
    63721 caaacttgcc aagaatcaag caggaaatcg aggctaaatc ttctatcaca aatttgaagc
    63781 aatcatttta acgaacatgg aaaaactatt ttacaacaac tattcatcta cactacttga
    63841 ttctatatga ttctataaaa aaagaagaga ctgggaaaaa ccagcctctt cttttttatt
    63901 tctatgcttc tgctgctgca ggagcttttc tcaataaacc ataaatcaca ccagatacga
    63961 ctgctccgat agcaataaac accaaataca tgaccgggtg actcagtaag aatacaacga
    64021 aaatcccgcc atgaggagcc agtaatttga tcccgaaagc tccgactaat ccgccagtta
    64081 aagcagaacc aataataaaa cttggaatgg cacgaatcgg gtcagctgct gcaaatggaa
    64141 tcgatccttc cgtgacaaac gatagtccca tcacgatgtt cgtcaaacca gcatcttgat
    64201 ctgttttgct gaatttattc ttaaacaaac gtgtagcaac gaaaattgcc aatggaggta
    64261 ccattcctcc agccataaca gaagccatca cgacacttcc gccagttgca acagtcgtag
    64321 ctaaagttgc tgtaccaaac acataagcgg ctttgttgat tggaccgcct aaatctgccg
    64381 ccatcattcc agctaataat gcaccaagta aagcagcatt cgaaccactc atacttgtca
    64441 agaaaccgtt caatgcatca ttgattgctt tcatagggac attgaccagc aacatcaaga
    64501 aaccagtaat cagtaagcca aacactggat aaaagaggat cgttttaatt ccatccaatg
    64561 attttggtaa gttcttgaat aatttacgta aaaatacgat gacataacca gctaagaatc
    64621 caccaatcag tgcacctaag aatcctgcac cgcctgtatt agctaatgca ccagcagcaa
    64681 atccaacgat caaaccaggg cggtcaccga tacttgaagc aataaatcct gctaataccg
    64741 gcagcatgaa accaaatgca gcttgtccga tttggttgaa ccagctagca gcttgattgt
    64801 aattccctag atttgataat tgatcatgtg gcacgccaat aaattggtcg atcatgaaag
    64861 aaagcgcaat agcgattcct ccaccaataa cgaaaggtaa catgtgtgat acgccgttca
    64921 tcaaatcttt gtaaattctt tgtccgatag taccatccgc atttccttct tctgaactgc
    64981 tttcttttcc atcaccatgg aatactggtg cagtaccact aagggctaga ttgatcagct
    65041 cttctggttt acggatacca tcactgacag gacgattgat cagttctttt ccgtcaaagc
    65101 gattcatctc tacttttttg tctgctgcaa cgattacacc ttctgcacgg gcgatgtctt
    65161 cagcagtcaa tcggtttttg attccttctg aaccgttcgt ttcgactttg atctccacac
    65221 ccatttcttt cgcttttttc ttcagagcat cttctgccat gtaagtgtga gcgatccctg
    65281 ttggacaagc tgtaacggca acaacgaatt ttctttctgg atgagcagaa gcttctgcag
    65341 cttgtgtttc ttgttctgct ttttcttctg cttctttttg ctcttcagct gtttggaaca
    65401 agtcttggac ttcttctggt gtatttgctt cttttaattt tcctacgaaa tctgcatcaa
    65461 tcagcaatcg agatagtgct gctaatgctt gcaaatgtgt gtcgtttgcg ccttctggag
    65521 ctgcgatcat gaaaaacaga taagttggct gtccgtctaa ggcttcataa tctactcctt
    65581 tttggctttt tgcgaataaa actgttgctt ctttaactgc actgtttttc gcgtgaggca
    65641 tggcgattcc atcaccaaga cctgtagaag tttgtgcttc gcgggctaga attccttctt
    65701 tatatgtttc gatatcggaa atccggccgg catcaaacat tttttggacc atttcatcga
    65761 ttgcgccttt tttatcagtc gcttccaaat ccatgatcat cgcttctttg accattaaat
    65821 ccttgatatt catgattatc ctccttgaaa ctaatcaatt tttgtgattt caacttctgg
    65881 cagcaactca tcaatcaatt cgcgagctgc caagtcatca gaaaaagctg ttgcgcttcc
    65941 gcaagcaact ccccatttaa aggcttcgat tggatctttc gtacggctgt aagccccaat
    66001 aaagccggca atcattgaat cccctgcacc tactgaattt ttcaatgggc gaactaatac
    66061 attcgtttta taagttcctt gttccgtgaa gagcaaagca ccgtctccag ccattgagat
    66121 cagcacattc tgtgcccctt cagctagcaa ttttttacca tacggaatga tttcttctac
    66181 agattggaag gttgtattgt aaagctcagc taattcgtga ttgtttggtt tgatcaacaa
    66241 tggtttctta acaagtgcat tttttaggtc atcacctgtt gtatcaatca cgaaatctgc
    66301 acctttttct cgaatgatat caatcagttc ttgataaaat ccttttcgca ggctagccgg
    66361 cgcgcttcct gacaatacga cgatatctcc ttgtcctact gttcctaatg cttgcttcaa
    66421 ctcatcgatt tcttcatctg aaatagatgg gcccaaaccg ttaatttcag tttcagtatc
    66481 tgattttagt ttgatattga ttcttgtatc ttcttttacg gttgtgaaca aagactggat
    66541 ttgttctttt tcaagccact tcgaaataaa ttcgccagtg aaccctccta aaaaccctag
    66601 tgctgtagat tgagaatcga tccgtttcaa aatacgggaa acattgatcc cttttcctcc
    66661 aggtaacttg aagtcttcct tcattcggtt caaatcgcct agttttaacc catctacgcg
    66721 tacgatataa tcaattgatg gattaagtgt tactgtgtaa atcattgtat tgcctcctta
    66781 attgttgtct tttcacggaa ttcattaacg aattctaatg gacaacgctc ggttatgatc
    66841 gtggctgctt ccaaaggtgc aacagaggtg aaggacaact ggttgaattt ggtatgatca
    66901 atcaatacaa atgtctcttc ggaatttttc atggctaaac gtttaacagc tgcctcttct
    66961 gggtccggag tcgtgaatcc ttgttcaaga tgggcaccgt tcattcccat aaatgcttta
    67021 ttaaaatgaa aattttttag ttgttctatc gcattagatc ctaaaacagc attagttgcc
    67081 agctttatcg ttccacctaa aataatggta gcaagctcca aatcaactaa atttgccgca
    67141 tgttgaacgg aatttgtcac tactgtgatc tgttttcctt gcagaaaagg aatcatttct
    67201 aacgtagtag agccagcgtc taaataaatg acatcttcgt cattaatggt ttcagctgcc
    67261 gatttggcga tttgctgttt ttcgtgaacg tttttgagtg atttttcact catattctgc
    67321 tcaaaattca ataattgctc tttcttagct ccgccatgaa ctctttctag aaacccacta
    67381 ttctccagct cttgtaaatc acgtcttatc gttgattcag aagcattaaa aaggtttaca
    67441 agatcttgtg atttcacgat tttctgctgt tcaagcaagt ctaggatttt ttgtctacgt
    67501 tcctctgtaa gcattttcac gatcacctca atacaaataa tagcatattt tttttctact
    67561 cgcaatataa atcagtcaaa aacgttcata aaaattataa atcagtcaaa aaaaaataaa
    67621 aaaccagtca aaaagactgg ttttttattt ttttgtctat catcaagccg ttttatcgtt
    67681 ttttattttc gcttcttgtt tttttcatat aaacagaagc aggttttctt tccttatgaa
    67741 gaagataata ataaagtccc atcccgccaa acatgaccaa aaaaccgatg actgatcgaa
    67801 taggaaaatt aggaacaaaa aagagacaga cggataataa tgatgccgga accattattc
    67861 gataatagag ttgtttatcc attttcttca cctcatattt tttgtaaagt aatcaattgt
    67921 tctaattctc ggtaatctaa aaattgcaca cgcagatgct gctcaccttt cttgacatcg
    67981 aattggatac ttcgccgctt tttctttatt cgtttgaagg taacatcaga gagtctatgt
    68041 ttgatttcct ttaacggtaa ttctgacagg gattctgaag gaacatagat tcttaaggat
    68101 aaaagatact cttgatattt ttcatctgtt acctctactt ctagatattc cctaatataa
    68161 taacaataag cttcgattcc attatccaaa gcaacagtcg gatcgataac tttatagcac
    68221 atcatgcgcg agcgattctc gtcaacccaa ggaaaatacg cctgagcatt tagtgttccg
    68281 cgaacacgcc tttctttata atctccttgt tgaatatcat aactttttgt tacaaaaaat
    68341 gaagcattct ttttaggaac aggttgcatt tgtgcattct gatactggac agattggatc
    68401 gtatggtcag tgtaaatgta ttcaacgaat ggcggtttca ataaaccgac accattgtcc
    68461 caagcaagat gatcaaaccc aagctggtcc ctaaaatagg attgaaacat ttgattgttt
    68521 ggcttttgcc agttaaaggg gtctcgataa agctggatac atatgacttc gttttctaat
    68581 tccttcactt tttctacttg ttggatgtcg agaaaaatcg gtttagggat gatttgatga
    68641 taatagcgac tataatacat ttcccaacac gaagtgaaac ataaaccttg gtaaaaaaga
    68701 tcgtatcctg gaaattggtt gcaatcgact actacttccc cgttttgttg tttcatttta
    68761 ggtaaatgtt cgatttcatc ctttttctca aacaatagcc gttgagagat ctgttttgta
    68821 ttgtgataga taaactcgct ataagctcgc atataggcga acaccccatg agcaatcatt
    68881 tttttctgaa tgaagtctcg aatcaaaaat tgcttttctt tgaaaacaga gatagacaat
    68941 acatttttta tcaataattg attatcttcc aagcgaaaac tacattcgtt ctcgtcattt
    69001 cctacaacta accaactttc gtctcttacc gatgctagca ttattttttc ttgaagcttt
    69061 cgaagatcat acggatccgc ttgttgtgtg aaagagcgga taaatgttgg ctggatcaac
    69121 agacgtgtca aaatagccca atcgcttatt tcctgttttt tttcttcttc atttaagtct
    69181 gtgagctgga tagtcatcaa taatttatct tcgccaatca ttttctcacc tctttaaaat
    69241 ctttttataa tttaattata accaacgata aagaaaaact aaaactttat gtcttagaaa
    69301 ccaatttgga cgattacatg atagactaag taaaaagaaa caaagaggtg tttacaatgg
    69361 ggttgaaatt tgaacgtaca gataatttag ataaactttt cgaatcattt gctgtcgatc
    69421 cggaaaaaac agttgcacca aaagaagaga tcgatcgatt tctaaaacca cagccacaaa
    69481 atgaaaagaa aaaagacaag aaacaaaagt aagttaagct caaaaaaaga cttaccagcc
    69541 aaacttttat gaaagagttt gactggtaag tcttttttat tctttttatg attcaattgc
    69601 ttgttcgatt gctgcccacg cttcttcttt tccctgtttt gtaacggaag aaaagagaat
    69661 aaacgtgtca ttgcgatcaa aatccaacgc ttttttgatt tcgctctcat gcttgttcca
    69721 tttaccacga gggatcttat ctgccttagt ggcaacaaca attacaggga tatcataata
    69781 cttcaaaaat tgatacatct ggatatcttc ttgagaaggc ttatgacgca aatctactaa
    69841 agaaacgacc gcacgtagct ggtctctagt cgttaaatat gtctcgatca tctttcccca
    69901 ttttgctcgc tcagttttgg atacttttgc atacccatat cccggaacat cgacaaaatg
    69961 taatgcgttc tcgatcaaat aaaaattcaa tgtctgggtc tttcctggct taccggaagt
    70021 acgggctaaa ttctttcgat caatcaatgt gttgataaaa gaagatttcc ctacatttga
    70081 tcgtcctgcc agagctattt ctggcaattg tgtatctgga tattgttttg gtgccactgc
    70141 acttatgata atttcagctt gatggacttt catggtctct ctccttaaca aggtgatgaa
    70201 taaagcaagc ggctgtcgca aaagccaaaa tgacttttac ggcagcctct tttcatcaac
    70261 ccgctttttt ctctgaatct ttatagatta ctgttggctc accagacaat tcagctgctt
    70321 cttttgtgat aatcactttt tcgatgtttt catttgaagg gatatcaaac atcacatcca
    70381 tcatgatttc ttcaatgatg gaacgaagtc ctcgagcacc ggtattgcgt tcgattgctt
    70441 ttttcgcaat ggctctcaac gcttcaggtt cgaattcaag ttcggtatta tctagagaaa
    70501 gaagtttttg atattgtttg actagcgcat ttttcggctc agtcaagatc cgtaccagat
    70561 catctgttgt caatttctct aaagctgcca taacaggtaa tcgtccgata aactcaggga
    70621 tcaatccgaa ttttagtaaa tcttcaggaa tgatatgctg catgacactt tcgtcttcag
    70681 aaagtttttg gttttgtgta ccaaatccaa tcgtcttttc gcccatacga ttcttcacaa
    70741 tcgtttcgat gccatcaaat gcaccaccta cgatgaacaa tatgttcgtc gtatcaatct
    70801 gaataaattc ttgatgtgga tgtttacgtc ctccctgagg tggcacgctt gcaacagttc
    70861 cttctagaat tttcagcaag gcttgttgga caccttcgcc ggaaacgtca cgagtaatag
    70921 atacattttc gcttttacgc gcaattttat cgatctcatc aatatagatg atcccttttt
    70981 ctgcacgttc cacattgtaa tcagcagatt gaagcaattt aagcaaaatg ttttctacat
    71041 cttcccccac ataaccagct tctgtcaaac ttgtcgcatc tgcgattgca aaaggtacat
    71101 tcaatgtttt tgctaatgtt tgtgctaaaa atgttttacc agaccctgta ggtccgatca
    71161 agcagatatt gcttttttgc agttcaacat catctgtgtt ttcttgtgct tgctgattga
    71221 cacgtttata gtgattgtat acggcaacag ctaacgagcg ttttgcgcga tcttgtccaa
    71281 tcacgtagtt attcaatact tcaagaattt ctagtggttt agggacttca gtgaattcgc
    71341 gtacagcttc ttcataaaat tcttcatcaa tgatttcttt acataggtcg atacattcat
    71401 tacagatgta gacccctgga ccagctacga tctttttcac ttcttcttgg gttttcccgc
    71461 aaaaagaaca gcgtacggtc tcattgctac ttggattgtc atacatggtc ttcacccctt
    71521 aacttgcaaa aagtatgcaa gaaactatca aagactttac agtcttttac tatcataaca
    71581 tagtttagta aaaagaaaaa gtgtttaaca aaggatcaac tgacctatac ctttgcattt
    71641 tttactctta acaaaaaata aggaactgtg acaaaatcgt gtcacagtcc cttatttcga
    71701 ataaacaatt ttctagaagt agcttcttca gaaatgattt attacttgat ttctattctt
    71761 tcgcttctgt cgctgtacct gtgatcaatt cgattgcttt tttcattgtc acatcatgtt
    71821 ccagcatatc ttctgtcaac acgcgtttga tttgttcgat tggcatgttg tatgtttctg
    71881 ataattcttg gatttctgca ttgatttctt cttctgttgc ttgaagattt tcagcagctg
    71941 caattgcttc gatcacaaga tttgttcttg tgcgcatttc tgcttcacct tcgaattgtt
    72001 tatgcaagtc ttcttctgta gaaccagtta attgatagta catttcagga gagatacctt
    72061 gacgttgcat gttgttcaag aattcgtcca ttgaacgatg tacttcatca tgaaccatca
    72121 cgtgtggcaa ttcaacgatt tctgcgtttt caacagccat acggatcgct gcttcatctt
    72181 ttgcatcgtc cgctgctttt tcttttgttt ctactaattc tttgcgatat ttttctttca
    72241 attcgtctaa tgttgatact tcgtcatcaa catcttttgc gaattcgtca tctaattcag
    72301 gcacttcttt tgcttttact tcatggattg tcactttgaa gactgcttct tttccagcaa
    72361 gatcttctgc ttgataatct tctgggaaag ttacatttac atctagactt tcacctgatt
    72421 tatgaccaac taattgatct tcaaaacctg gaatgaatga gcctgaacct aattcaagtg
    72481 agtaattttc gccttttccg ccatcaaacg cttcgccatc tacaaagcct tcgaagtcga
    72541 ttactactgt atcgccattt tcagctgcag catcttcttt gataactaat tcagcttgtg
    72601 cttcttgttc acgtttgata cgtgcatcca cgtcagcatc tgttacttca cggtcttgtt
    72661 tttcaacaac tagatcttta tattgtccta attttacttc tggtttcact gttacttcag
    72721 cagtgattac ccagtcttga cctttttcca tgctttcaac atcgattttt ggttgagaaa
    72781 caggatcgat gccagcttct ttgacagcag cttcataagc ttctggtaat acagcattca
    72841 atgcatcttc atataaagct tcttcaccgt acatacggtt gaatacttga cgagaaactt
    72901 tccctttacg gaatccaggt acgttcaagc tttttttcac tttattaaac gcagttgtta
    72961 atcctttttg gatttcgtct tgcgcaatcg taaattttaa aacaccatca ttcgtgcctt
    73021 ttttttcaaa tgttgcagtc atttaattcc ctccgagttt agaaattcat tttatacatc
    73081 aaaaacgttt catgcatacc tttaaatagt acactaaccc tttcaaattg taaacatatt
    73141 gaatggatag aatccttgtt tttcataaca tttttatctt tttcactgat aaaatctaat
    73201 ggaagaagat catttgctgc aaaatttttt ctcttttttt taaaaaatct tgtttttcat
    73261 cttctgtacc ttcatacatc gtttttcctg tacacgcctc tacagtcagt tgaaaccagc
    73321 gcttcgggtc ttcgatgtat gtatcataga caggataaac aattgcagat tgcaacgtga
    73381 attcttcatt taacacggaa ctaagcagcg catcttcatt accgtaacga tctgaaaata
    73441 cacataattg ctggtaactt gcctgctgct gcggctttgg cagatctttt ggtatgactt
    73501 tgactaactt ttcttcaatc gtcaaataga aaacaggatg gcattcgttc aattgcctaa
    73561 gtgtctccag taattttgcc cttgctaaaa tatggacctc tggcgataca ataagtttac
    73621 tcgctgtttg gataaactca tgataaggaa gctgttcgat ctcatcaata gacgcaagct
    73681 gacgtgcagg ttccaacgaa ggaagttcct ccgatttatt gtggagtgcc ttgatcttct
    73741 gcctctccat ttgtccggat aattcttctg caaattggat tttttcaatg acccgctgct
    73801 cgaaagcttc ataaaaatct gtacttgcta tcaattttct agcatataaa aaatcttgtg
    73861 tactgattaa caagtcaaaa taaaattcag caaactcctc attttgaaga tactgttttt
    73921 catgcaagag tgcttgcttc aatgcttctt ttttctcgtc caactcgtaa agacaaaaaa
    73981 caagcaggct attagcctca aagctttctt tcaactcgta agctcgttga aataattctt
    74041 ttgcctgctg atattgctgg tttgctagtg cttgttgtgc tactcgcatt aaattctcat
    74101 atttttctgg aaaatcaatc ggcttcacgc agtgtcctcc tcttgctcat gttcagctca
    74161 tgttcaatag tagcaaaaca aaaaaagcat gacaattaaa aataactgaa gcaccctcta
    74221 aaagctagat tttagatctg acttttagaa ggtgctttca actgttactg ctttttattg
    74281 ctcatcagct gttgacttcc tggttgaatg ctttgatcag attgatcgta acaacacttg
    74341 tattagtgaa aaaatcgcct agatgttgct ttttctgatt aaagattgct ggaagatacc
    74401 ctaacatcag caaaggaaat tttagaatga accggcaagc accttctcta actaaaacgg
    74461 ttgaccagct aagttcttct tcatcaaagc tgatgactcg aataccaaag atcattttcc
    74521 caatcgtttg accatgattg aatttcgtca ataaaataaa atacgctaga taaataagta
    74581 aggctgtgaa tccgtagata ctcagataag aatctgaaaa attaagccaa cctagatttt
    74641 tggcaatacc aagtgttaaa cgcgtaatag ctccgataca caaaagatcg accaagaaag
    74701 caaacattcg gataaaaaaa cctgcataga aatacttagg aaaatcattt gcttttggcg
    74761 gttcatttct tgtgtccgta tactttttcc atttacgttg tttctttttg atttcttcgg
    74821 gcgtcaaagg ctctctttca aaagagctca gtaccttttg ggaaaagttg gagtcaggtc
    74881 ctacagcagg ctgctgttgc ttatcttttt cttgttcctt attttcattt gaaagctctg
    74941 acatgcttac tcacctccgt aataatacat tgcttttggt gattccgctg tccctagatt
    75001 ctcaagaacg gataagatct gactagtctc agaaggcttc aatccctgga attctgctag
    75061 tttacttcct aaccatgtac tagcaaaccc agatggcgtg gaagaatatt ctactagttc
    75121 cgcatcttca agctgttgtt cctttcgcat agcttttaaa gcatcttcag gaaatcccaa
    75181 ttcatcgact aaacctactt cttttgcttg gacaccatca taaatccttc catcagcaat
    75241 ttttttgact tcttcttcag atttattccg tccctcgctg acgattgcta caaaacgact
    75301 atatgcgctg tcgatatatg cttgaagcac cgcttcttct tcttttgtct ctggtcgcgt
    75361 tcctgaaccc atatctttca acgctccgct tttaactgtt gtatcttcca cacccagttt
    75421 ttctagaagt cctgaataat tcaatcctga catgatcaca ccaattgagc cggtcactgt
    75481 ttcttctgtt gcaaagattt tatctgcttg agcagaaata tagtagccac cgctagctgc
    75541 catgtttttc atgctgacat acattgggat atcatgttct ttacgaatcg tatccaattt
    75601 cttagcgatt tccgcacttt cgtagacacc gccaccagga gaatttactt ctaaaaagat
    75661 acctttgact gttggatctt cttcaatcgc cttcagttgt tccataaaat ttttatgatt
    75721 gtaaccttca gaagcaaata aacctgaaga ccctcccgca gcaatcgttc catctactga
    75781 taattttacg attttttgcg tactgtctcc ctcttccaac acatttgaag ctagttcttc
    75841 atccccatat aaccaactgt tcaatccctc aatttgatcg ttttcctgag gcttagtcaa
    75901 actagctgaa actaacgaaa ccacaaataa tcctgcagca atcagaactg ctgcccaacg
    75961 tctcttattc attctttctc ctttctaata caagtttttt atgacgatca cagtttctga
    76021 ctgctcagaa gtgtcgaacc gctctgcttt ttcttcccgc gcatacccgg aaaacgactc
    76081 ttttgtcaat tcatcttcta ccgtaaactc acgagcttca gcaacttcca aggcatcagg
    76141 tgcgatttct tggatgctat cccagccagt atctaaaaat attttcgtat catctgatac
    76201 accagctgat gcttcgaatt tggacaattg tttttttaac attcccagtg tcatgatctc
    76261 aatttttgaa tccattttta ctcctcctct atccgattac tttctattgt accgaactcc
    76321 tgaaaaagat gatagataaa aacccattat tttttgacat aaaaagagca tttgacaaag
    76381 catttgatat ataccaattt attttttatt ctttttttgt tgacaagaac attcgcaaaa
    76441 ggttatgatt ttaaatgttt ccagataaaa aacaaaagag gaggatagtt atacctttta
    76501 ggatagcgcc aggtctgtgt aaacagatga cgaggagagg gtttatcgaa agattcggcg
    76561 gatggcccta gggactgcac tctacagaaa atccacaaaa aatatctgca aagataaaac
    76621 aaaaggattt tcctgcagct ggaagcattc atacgaattc attatagaaa gtaggatgtt
    76681 ttttatgtgc ggaattgttg gaatgattgg attaaaaaat gtaacaccag gattgattga
    76741 cggtctagaa aaattagaat accgtggtta tgattcagct ggtatctttg tctcagatgg
    76801 cacaactgat tatctggtca aagcacaagg cagaattcaa aatttgaaaa acaaaataaa
    76861 tattgatact acaggaaata taggtattgg tcatacacgc tgggcaactc acgggcaacc
    76921 ttctgaagaa aacgcccatc cacatacttc ccaaagtgga cgcttcgtgc tcgttcacaa
    76981 tggcgtaatc gaaaactttg aagaacttaa aattgcttat ttagcaaatg accactttat
    77041 tggtgagacc gatactgaga ttatcgctca tctaattgaa actttcgcga aggatactac
    77101 aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa ggttcttacg ctttcgcttt
    77161 gattgatcgg acagcacctg atgttattta cgtcgctaaa aataaaagtc cactattaat
    77221 cggactcggc gatggattca atgtgattgc cagcgatgca atggctatgc ttgctcatac
    77281 gaaggaattc gttgagattg aagatgaaga gatggttacc gtcacatcag aaaaagttat
    77341 tattcaaaat tttgcaggtg acatcgtaga acgttcttca ttcgaagcac aagtagattc
    77401 ttcagatatc gagaaaggta cctatccttt ttatatgctg aaagaaatcg acgaacagcc
    77461 tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa gacaatcgtg tctctcttga
    77521 tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc tatatcgttg cttgcggaac
    77581 tagttaccat gcggggttag caggtaaaca gactctggaa aaattgacac agatccctgt
    77641 tgaggttcat ttagccagtg aatttggcta taacacacct cttctttcaa aaaaaccatt
    77701 ctttattttc ttgagtcaaa gtggcgaaac agcagatagc cgccaagttc tggtaaaaat
    77761 caaccgtctc ggctaccctt cacttacgat tacaaatgtt gctggttcta ctctttcgcg
    77821 ggaagcatcc tttacactgc tattgcatgc tggacccgaa atcgcagtag catcaacgaa
    77881 agcttatact gcacaaatag ctgtactaac tttattagcc aaagctatcg gtgataaaaa
    77941 agaatttgaa acttctcttg tattcgatat cactcatgaa ttgagtttag ttgctaatgc
    78001 gatggaatcg gtcatcgccc aaaaagatta tttagaagag ttggcagaag tatatctcag
    78061 cgatacaaga aatgcctttt acattggacg tggcgctgac tacaacgttt ctcttgaagc
    78121 tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa ggttttgccg ctggcgaatt
    78181 gaaacatgga acgattgctt tgatcgaaga aggaacacca gtgattggaa tcatcagtga
    78241 agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa gtcgaaagcc gtggcgctaa
    78301 aacactagtt attgtctcag agggattaga aaaggaaagt gaccagctgg tgctaccaac
    78361 tgtccaccct tatctaacta ctctaaccac agtcgtaccg actcaattac ttgcatatta
    78421 cgcaacgcta ttacggggat tcgacgttga caaaccacgt aatttagcga aatcagtgac
    78481 agtagaataa taaaagaaca aggaccagtg tcagacatct gacactggtc cttgttcttt
    78541 tattttttat gaaaatatct cttaatccag tccgatttct tctttgacta ctgccgcgat
    78601 tttatcgaca tagtaattaa ctttctcatc agtaggcgct tctgccatca ctcgaagtaa
    78661 tggttctgta ccggaaggac gaactaggat tcttccttct gatcccattt cagcttctgc
    78721 ctcttcaatc acttttttga ttgccggaac atccatcgca ccgtttttat cacttactcg
    78781 gatattcact aatttttgcg gatagattgt tacctctgct gcaagctctg ataatttctt
    78841 acctgtctgt ttcatgatat tcagtaactg aattcctgaa agcatcccat ctccagttgt
    78901 gttaaaatct aagaaaatca tgtgcccaga ctgctcgccg ccaaagttat aatcgttttt
    78961 gcgcatttct tctacaacgt aacgatcacc aacttgtgta acgacatctt tcaaaccgat
    79021 tccttccacc gctttgtgga atcctaagtt actcatgaca gtcgtaacga tcgtatcttt
    79081 tttcaatcga tttttttctg ccaagtatct agcacagata aacatgattt tatcaccgtc
    79141 gacaatgttt ccaagttcat ccaccgcaat aatacgatcg ccatcgccat cgaaagctag
    79201 tccagcatct gctccttttt caacgaccat ctcagctaaa cgttctggat gggtagagcc
    79261 tacgccatca ttgatattta acccgtctgg atttgtgccc atggtataaa aatctgtttc
    79321 aagatcggca aacaaacgat taacacttgt tgaagttgct ccgtttgctg catcgacaca
    79381 aatggtcaaa ccggaaaggt caccgttgat cgtttgtaca aggaattgag aatatttcaa
    79441 taagccttct ggaaattctt ctacagttcc caatccttca gctgatggac gaggcaattc
    79501 atccacttca gcatctaata atgcttcaat ttctagctct tggtcatcta ctagtttaaa
    79561 accatctgca ccgaaaaatt tgattccatt atcttgtgct ggattatgag aagcagaaat
    79621 catgacccct gcgcttgctt tttgcaaacg tgtcagataa gcaacacctg gtgtagagat
    79681 cacacctagc tggaacactt cgatacccac cgataaaagt cctgaaatca gcgcttcttc
    79741 taacatttga cctgagattc ttgtatcccg ccctactaac acgcgtgggc gttttccttc
    79801 ttcttcatgc tggcttagga cataacctcc gcaccgtccc agcttaaatg caagttctgg
    79861 cgtaagttcc ttgttggctt caccgcgtac gccgtctgtt ccaaaatatt tacccatagt
    79921 tgtaaaatta ctcctttaac ttaattttct gaggaatttg ttctgctcat ttgtgttgat
    79981 tcgcttgttt ctgatgtttc ttctgtactt gtggtcgagc tgcttgtccc agattccgaa
    80041 ctggaagaaa tggttgttgt cgatggttcc gttcctgaat cagcagatga aatacttcta
    80101 ctcgtacttg tattttcttg tgtccgactg ctcgtcgaag cagagctact gcttgacgtt
    80161 tgcaacacgg gtgtaatctc cacactaact gattcgggct tgatacttaa cccctcttct
    80221 actggtattt caattttacg agttgttcga tgattgatac ctgagacatt gatcggtaca
    80281 gcaatactgt caccgatcga tgcaagcaaa tcgctagagc cgcttacttc tgcttccagt
    80341 tttgaaaaac tgaaatcata gctttctacc ccggcagctg gtgttccttg ctgtatccca
    80401 taaagtcgta ccgacttgct tggcttgatc gaatcagctc tcactgttac ttgcactgga
    80461 tcagagacga tacttaatac ttcccctgct gcattcagcg cctgtattgg gacctcacta
    80521 ttaatcccat catctgtgac catcgaagcg tccacatcag ctactactcg tttgatttct
    80581 gccaatgttt tgtcacctgt agtgatagag acttttttag gactaacgga agtatcatca
    80641 agttcatatc cagatggtgt gaacgttgaa ggaatcaacg tttccacatc gaaatctttt
    80701 attacctttt tctcaaccgt tactgtaata gtcgatggtt cgatctttgc tgtgacagca
    80761 ctgctcaaat tttgaatttt tagcggaacc tcatgtgttc cttcgcccag cttagtgata
    80821 tctgctgtga cgcggaacat ccgtgtatct tcgtttgcct ctgcattcaa ctgaactcgg
    80881 tttgcactgg aaagtttgac agtcactgca ttttcatatc catggatata gtagcgcttt
    80941 gaatcataag aaatcttgat tggcacccca gtaactgttt gattatacgc ttcatttcca
    81001 gaaagcgtat ttggaacgga ctggccattt gcattgaagt acagcgcaat actgaacagc
    81061 aatgcaataa aactgtaaag aatgcttgtc cgtttctctt ttgagatcat ttgcgatccc
    81121 ctcccttagt cataccgtct aggaaagatt gaatcacgtt tttcttgttt tttgttttct
    81181 cttcagcaat cagttctcta cgcaaaattg ccaaatattc ttgctgggat aagccagcca
    81241 tcagttcatt atctaatgta atgctgacat ccccagtttc ttcagagaca acgatggtaa
    81301 tcgcgtcgct gacttcgctt ataccgacag ctgcccggtg tcgagtcccg aattcttttg
    81361 gaatcaacat actttctgat agaggaagat aggcgcttgc tactgcgatc tttccttctt
    81421 ttacaataac tgcaccgtca tgcagcggtg tattaggaat aaagatattg atcagcagct
    81481 ctccagtgat atctgcatct aatgcaattc ctgtctcaat atactcatct aatccagtat
    81541 gccgttcgat agtaattagc gcaccgattt ttcgcttgga catatattgg atggctttgt
    81601 caaaagacaa gatcatcttc tcatcttcct gctgttcgct ttttgattgt ttgaaaagcg
    81661 aactgcgtcc aagatgttcc agtcctctcc ggatttccgg ctgaaaaatc acgatagcag
    81721 ctatcacccc gtacgtaatg acttggttca tcagccaaga caaggtgtgc agaccaatga
    81781 tttccgctac gattcggata acaataaaaa tagctacacc tttcagaagc tgaatcgctt
    81841 ttgtgcctcg cagcagttgg atcagtttgt atacaagata ccagacaacc agaatatcaa
    81901 gtatgttcac caaaaaattc agagagaaca agtcaccgga aaacatttgc tgccaatagt
    81961 ccaaattaaa aagttccccg atttgaaatg acataaaaag cctctcttct cttttgagat
    82021 ttcctctcta gtatatcata tgttttaatt ttagaggctg atctgctcgt atttttcttg
    82081 agaaaaacgc ataattttca ggaaaattgc aagtatgcgt tttatttttc tctgcaataa
    82141 atatttttaa acttcagaga attattcatt atattttttc tgttattttt cctttttttc
    82201 ttttgtagct tgttccattt ttttacgtcg ttctgctctg gcagctcttc tttcttctgc
    82261 ttcaaggtcc gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg
    82321 ttcaagtcgt ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg
    82381 tgttttagcg tcttggatag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt
    82441 ctctagcgag gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg
    82501 ttttttgtag tccaaagtca ttgggttctt gtttttttcg accattgcag ggttatatcg
    82561 atataatgac caatagccag aagaaacagc ttcttttgct tctttaagcg attctttcat
    82621 tccaccaagc aaaccatgtg tgatacaagg cgtataggca atgattaaag aaggtccagg
    82681 atacttttct gcttcttcaa aagcctttat cgtctgcatt tgattggcac ctgaagcaat
    82741 ttgcgccaca taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc
    82801 atactttcca ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg
    82861 accgccagta ttggaataga cttcgttatc caaaacaaga atattcacgt ctgctccgct
    82921 ggcaagtaca tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat
    82981 catccattgg ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg
    83041 gacaattgat cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc
    83101 ttcgctctca aacatgtgta ccaaccagtc ttccatcaat tctcgtagtt cttctgatac
    83161 ttttggcaat acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag
    83221 aagcatacca taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc
    83281 ttgaccacat tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga
    83341 acagcctgtc gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac
    83401 ataaggtgtt tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa
    83461 ttgtgtagac aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat
    83521 tgcaaaagcc caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc
    83581 tttttctttt gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga
    83641 aacttggata cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc
    83701 tggcgcttct tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca
    83761 aacaaaggca cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa
    83821 agcgacgccc cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc
    83881 atttgctaca agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag
    83941 tatttcttgt atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct
    84001 ccaatggtct gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat
    84061 gtttttttct acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag
    84121 cagcggaagc acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat
    84181 tgccgtattg atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt
    84241 atagaatcta atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc
    84301 taattgttcg tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt
    84361 gaccaagtca tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc
    84421 gacaagatac gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc
    84481 gccagatttt ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc
    84541 aataatcttg atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa
    84601 cttggcctgg aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg
    84661 ataagtcaca tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt
    84721 tttcaattca tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc
    84781 gtagcgacca ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc
    84841 taataagaga ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt
    84901 ttttactgta tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg
    84961 gatattcaaa aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat
    85021 cgtctgtgca actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata
    85081 agtcaccaag tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac
    85141 gatttcaggt aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc
    85201 tggattctgg ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa
    85261 tgaagctaat ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat
    85321 tttttgaatc tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag gtacttctcc
    85381 ttcaatagaa gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc
    85441 agctaacata gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat
    85501 attcaacgca tttgtcgtca ctgcccgact agcaacatgg aaaacacttg gcagtagttc
    85561 cccagcaatc ttgtacatat tcggtatcat caaaagcagc ccttgagaag cggtatatgt
    85621 agttgtcagt gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga
    85681 ttgcatttct actactttta ctggttgacc gaatacattt tcttttcctg ctgctgacca
    85741 ttgatctact aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc
    85801 ggtaaatgca taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg
    85861 catgattcat catccttgtt catattctct cttattatct gttccattat aacgtaaaac
    85921 attccatttt ttcttttatt tataaaaata tttcacatat gtctatttga attggtcatc
    85981 taatttttag acaaacagtc taaatcgttg cctttttttg tcagacatgt ataaactata
    86041 agcgaaagag gtgttttaac atggatatat cagtgattga tgcaacgaaa acaaatacac
    86101 aaaaaggaat cctttatggt tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaacc
    86161 tatcttgtcc gtattgccga caatggtttg aagaatctta tgagctatta gaagaagcag
    86221 tccaaagcgg gcaattgcag cgagtgatca aattgttcga taaagaaaaa gaaagcttgc
    86281 agcgaggaaa cgtgatgcac cggtatctca caatcagcga tggtcaaaaa gcgatcaaag
    86341 agatcaaaca aatattcgat actcaagacg agtggaaaca tctttctctc caagaagtag
    86401 cggattttgc agtagataaa ctaaagctga cagaacaaaa agacgaacag ctgagccaag
    86461 ctgtcatcaa cgaagctgag caagcacata ttcgatttgt tccgactgtc atcttgggta
    86521 aagaaatctt tgatgaatct atttcaatag aggaattaaa agaattgatc caaacaaaat
    86581 aaaaaaataa ggagcgtagg acaaaagtaa agaacacttt tgtctcacgc tctaaacacc
    86641 tataaacggc ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc
    86701 cagcctcctt attccgaata aatggtgtcc aaaagcttgc atctgccaga ttatctcatt
    86761 tcttatgaca agctgagcaa attatctcca ccaaataatt tattcatatt tttgacttgc
    86821 gcatactaac taattcttaa attttcatac aaaatttctc aaattccctt aacgagtaat
    86881 atttatgtac ggtgattcac actagtatcc caatcacttt ttaagttatc ccgatttcaa
    86941 ttctacttca aaaatagttg cttttatgta tgctgttagt tgattagatg gtcgtttgtt
    87001 gagctaggtc gtttatttta aacaagttgt ttctttagct tatacctatc tcgtctacca
    87061 aacgcaagat ggcaaacaca aatgcatacg catctgtatt tgccactttg attcgtataa
    87121 agctaacaac tcctaactcc tctcatttaa tagttgctct cttttttatt cagatacatt
    87181 tttgctgatc acagcctagt atttcaatgg ttttgaacac taaaccaaat tgttccatta
    87241 caacattcac tggctgatga aatggattaa tagatttcga ttattcaaag cgattttcta
    87301 ttggtggatt gtgttcccaa ctaacaagac ggattccaaa gtcattatat aactcttcaa
    87361 gagttaaccc tttcctgttc ccaatagatg tatctctccc acgctcgata taaaatatgg
    87421 agtttcgaat gccaagacct ggcaaatctt tccttgttac agttacactg ctactaactt
    87481 ccccatataa aatccagctc ctccaaaatg ataatatatt tttcgaccat catctaactc
    87541 atgtaacaat aattgttgtg ccctcttgtc actcgccaaa tgcaaacgcc acgtgcgata
    87601 ccatgccatg ctacctgcat ctgcagaaaa tccatcaaat aatgcaacaa ttgtatcttc
    87661 tatcacaatc ctttccccat ctacttcata ttctaatcta aaatcaaact cccctcgggt
    87721 aatcgcaggt cttggtggct taggacccaa tattattcta cttaaaaccg cattcgttcc
    87781 cagatgaaac acagctataa caacaataat gattaatatt ttgtttctat ttgataaatt
    87841 tctcattatt cgcaaatcac ctctacattc tcacatacta gttcgtaata ctcctttaat
    87901 atcaaaatat ctactgatcg taataatatt cgctatgaat tcctactact cgtataagaa
    87961 aggccaaatc atcagctttt acttgctgat gatttggctt gtttctattt atcgtaattg
    88021 ttttctaatc accaaaattg ttaacaatcg gttcagatat ttcccaattc actagcctta
    88081 tattatattg atttaacaat tcttcttcat ttattgtatt ttgatcaagc ggaccaaatt
    88141 gttctaataa ttctttgtca tgtaaaatat cccttctagt aatgcctctt tccctttcaa
    88201 tgaaagaagc actaggaaaa gtatgattat aaccattcaa cctatctccc atgtaataat
    88261 tagcaggtcc tggaaaataa aatatttgct tattatcact aacttctaac agcaacacta
    88321 aatcttctcc gctggctaaa cgacttgtcc atctacgata tctccctatg ccttcgttca
    88381 ttccaaatcc atcaaattca actattaacg tgtcttctat cacatgtaat tcttcattca
    88441 attcatactc aagtctaaaa ggaaattctc catatgtaat ctctggcaat ggtggtgctg
    88501 gagttctcca aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg
    88561 ttatgagacc tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta
    88621 tatctagaaa aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaaacgca
    88681 tttttgccct ccctttataa acatcgattc tcaaattcta aaccaaatct tggtagttca
    88741 agttcaggta taacgtattc caaccataca tctcaattac tatttatgaa cttatgatta
    88801 tcactgaata aatcactcca tccccttgtt aaaacttcta cattaattac acctggttgt
    88861 tttcgactag ctaatccaat gaacttatca accatcctta tccaccaacc acgagaatct
    88921 tgaccagaag taagaagagc agttctcaag gatgatcgag cagttccttc cagtttttta
    88981 attaatcagg ccagttatag ataaatactt gatcttatct atatcaggac ttctatatta
    89041 agcaaaaatt tatgtacaaa actaaaatct tcattcagtt gtctccccgc tttttatacg
    89101 attgtattaa aaacaataga agcctttcca aattatgtta ctccagtaaa gatggaatta
    89161 tagtttgacg gaagaaaagc aagtataact tttaaaaaaa actgatacat tcgcatctcc
    89221 tgcttcatgt atcagttttt tctttggatt aaactgcttc ttcacgcagt ttttccgcaa
    89281 attcatttat tttgcgaatt cggtgattaa ttcccgattt tgatatcgca cctgagggaa
    89341 tcatttcccc taattctttc aagctgacct ctggatgttg aagccgaagt tctgcgattt
    89401 cctgaagttt ttcaggaagg gcatgtagac cgacctttgc ttcgatcaat tcgatgtttt
    89461 cgatttgttt agaagccgca tcaatcgtct tgttcagatt tgctgtctca caattgacta
    89521 gacgattgac cgaatttctc atatcccgta cgattcgaac atcttcaaat cggagcattg
    89581 agttggttgc accaatcaat gttaagaaat cagcaatttt ttctgctcct tttaaataag
    89641 aaatatagcc attgcgccgt tcaagcgttc gagcatgtag accgtaataa tttaacatat
    89701 cgcaaatgtc ttggttatgc tgttcataaa tagaaaatat ttccaaatga taacggctcg
    89761 tttccggatt attgactgaa cctgaggcca taaaagcacc acgtaagtaa gaacgcattt
    89821 tttgcgaatt tcccataatc tcgtccgaaa cgtgtccatt gaacatcata ccatccatga
    89881 tatcaagatc catcaacaca tcttttgtgc cttgcttcaa ccggacgata tagacattgt
    89941 tcttttttaa tttcattttt ttacgaacga gtagctcact tcgtacctga taatgatctt
    90001 tcaaaagaga atagatacgt ctggcaattg cagcattttc tgtttggacg ttcaaaataa
    90061 actgctggtt cacaatacct aaagagccgt tcattcgcag caaagcagct aattccgctt
    90121 tcgcatgttc tcgatgaact tccaaaccag ttagttcttt cttcacatct gcagcaaatg
    90181 acatcctatt ctcctccttt ctccaacaat aaattgctta atattttgaa ccgaaaacta
    90241 aacggaacaa ttcttctact actttgtctc catcgtggaa gacaccacca tctttcaatt
    90301 ccaaaaagtc ggtggaaatc actcgacatc cttcatttct caatccgttg aagtcgtgtg
    90361 cgacttgtac tagatactca tcatagatct ctgggtccat ataatcttca ggcacttttt
    90421 ccgtattgac caatactgta tcgataaaag gctgattcaa atgcttgtgt aaaacacgta
    90481 tatgatcggc atctgtgaaa tgttccgttt cccctttttg ggtcatgata ttacagatat
    90541 agacggtttc agcagttgtc ttgagcattg cttcgccgat ttctggtatc actaaatttg
    90601 gaaggatact ggtgaataaa cttccgggtc ccaaaacgac catgtcggcg tccataattg
    90661 ctgaaacgac ttttcgagcc gctttggctt cacttttatc atttgtgttg cagacataca
    90721 cacggtcaat ggttttacgg tcgatcgcga ttttagattc tcctactgca gtcgttccgt
    90781 ctttaaagac agcatgcaag gtcaaaggag tttctgaaga aggataaaca tgtccatcta
    90841 catgcatcat ttttgctaat aattgaatcg cctcataagt gcttccccgc atctcggaaa
    90901 ctgcggcaat gatcagatta ccgatcgtat gattggctaa aaactgatct tcctttttga
    90961 aacggtattg gaaaatatct tcatagaatt gtggcatatc cgataacgcc actaagacat
    91021 ttctcaagtc accaggagga gccatagcga tcgattctct gattgcaccg ctgcttcccc
    91081 catcatctgc aacagtcact actgctgtga tatctgctcc ttgatttcgc aaacttttca
    91141 ggataactgg cagaccagtg ccaccgccga ccactacgat ctttggtttt cttatccggt
    91201 aggttttcat tttcacgacc ggttcaccgt ctctttcctt ttatccttgt cccgatgtgt
    91261 cacattgact ttatagtttt tccctaaagc atttgccaaa cgttcagtca atgccacaga
    91321 acggtgttga ccgcctgtac agccgattgc aactgtcaag ctgctttttc cttctttaat
    91381 gtatccaggc aatacagttt ccagaagatc caaaaattta tggtagaatg attgcgtttc
    91441 atcaaaggac atcacataat catagactgg ctgatctttt ccggtcaatg gtcttagttc
    91501 cgggatataa tgagggttgg gcaaaaaacg cacatccatc acaatatccg aatcgatcgg
    91561 caacccgtat ttaaaaccaa atgatacaaa ttcgatccga aatccttggt ctccctttga
    91621 tttgaatgac tcattgattt tttcacgtaa ttgtcgtggt gtcaatgtcg tcgtatcaat
    91681 gatcacggaa gcttgggctt tcaagtcgtc tagtatcgcc cgttctttac gtatcccttc
    91741 agtgatcaaa ccgtccatcg ccattggatg tgctcgtctt gtttctttgt agcgagccac
    91801 tagttcttcg tctgaagcat ctaaaaataa gatagaagtg tcgatgaagt tcgtattttc
    91861 gatttcgatc agcatgtttt ggatttcttc aaagaaggaa cgagaacgta aatccactcc
    91921 taaagccatt tttgtgactt tgcctgattc tttgattaat tcccagaatt ttggaatcaa
    91981 tcgaggcggc atattatcga tacaaaaata gcccatatct tcaaaacttt ggatagctac
    92041 tgttttccct gcaccgctca tacctgttat gatgactaat tgcaaattat ctgtcataat
    92101 tacgaactcc tctcacattg tgcatagtaa aattatatca ttccgggcgt atcattacca
    92161 gttttctttt tcttttacaa aaagatttgg accaaatatc ttttaggata tttgccccaa
    92221 atccaatttt tttacttttc cgatgctgta tccaatgcct tgcttctttc agctagcgct
    92281 cgttcaatcg tccgatctgc taaaatagat tgggcatcga tcactggata atggttatct
    92341 tcagcaaatt catgcatcaa tgacaattct gtgcaaccta agataacttt ttcacagttt
    92401 aggcgctcta ccgcttcttc taagatttcg taatatagct cttgattcaa ataatccgat
    92461 tccttgatct catgatagat cagataattg attttttctt gaagaacagt atcaggaatc
    92521 actgtttcga atccaagatt tttgacttcg cgttcataga ttccagcttt catacttcct
    92581 tctgttccta gaatcgctac tctccctgtc gtatgctgac ggacaagttc atttgctgct
    92641 tctcttggca tatgaaggat aggaatatcc gttgccgctt gtaattcttc aaaaaagtag
    92701 tgtgcggtgt tacaagtcaa cacaatgaag ttcggctgca ataaattttg tttctcaata
    92761 tcatccagca gaaacggcat tggattttct tctgatcgat ctaaaatata agctgtacga
    92821 tcaggaactg ttgcatgatt gaataagaca taattcaaat attcttgatc ttttgtagct
    92881 ttcgttcggt ggttgatcaa gcgaacaaaa ctttctgtcg ccatcgtgcc cattccgcct
    92941 aaaatactga aaaaattctc catgaactag ccttctttca catgaaaata ctttttgaat
    93001 cttggaatat agttatggaa catatatttc atcaacaacc agcgcttgat tgaccggtct
    93061 ttttcataaa acacagtagt accgtaacga ttttctttga tcatttgctc agctagtttt
    93121 ttgtcttcat tttcccgagc atattctaaa aagatttttt tgggcacacc catccataat
    93181 cttgctttat ctgattgatt cgttccatag gtcgtttcaa caaagggttt gttaaacact
    93241 cgatcttctg tgacgaaacg agccagattc aagccgttca atgtcacaaa gaaactgcta
    93301 cgaccttgtc tcaaattgat ttcaaataat ttatattctc catctctagg atcatacttc
    93361 atatcgaaat tggcaaagcc tgtatattcg attttttcca aaaatgcctt aatggtttga
    93421 tagatttttt cgttataatc tggcatgatc acaacgtaat taccaatcga tgctggtgta
    93481 ggatcttcta acagtggatg accaagacac atcatccgaa cttgatgatc ttcatctaca
    93541 tacgcattta gcacacgcat attactatca tcacccggga taaaatcttg tacaatcatt
    93601 tcacttttat aaccggcttc gtatatccgg cctaaaatta aatcgaactc ttctctagtc
    93661 tctaaaataa aagctttttt tcttccttca aattggacag aaagatactc cacactgttt
    93721 gcaggtttca aagcaactgg gaattcaaaa ggcaattcct gttccaaatg tccatttact
    93781 aacatttctt ctctgacgat caatgtttta ggatacggaa gatcatattc ttcacaaact
    93841 tcataaaagc ttactttatt gatcagtctt tcaaataaag aataatcaat atatggacag
    93901 ataaaatatt ctgacagctc ttgtttatgt tgggagatca attctgcata tccatcacca
    93961 caagcaatca aaagatattt tttactctta tcagaatatc tttcttttgc caaacgaagc
    94021 atcgtctcga taaacacagg gtctttatca aatcctggaa taacttctac attgacaatt
    94081 ttactgtagc gggtaggggc taactgatcc gaagcatatg cttggcagat tttgccgtat
    94141 gcttcattaa aagaacgtgc cataccataa acgttcatgt cgcttcctaa taatataggg
    94201 ataaattctt cattttcaat actgttcatc attatctctc acttactcat aatctcttgt
    94261 ttttcttttc caactacaca aggaatatca taccataaag aaaaaagcaa tcaaaggaaa
    94321 ctcctccgat tgctcaattc ttttttgaac ggattgaact tataggaagt ttttgtctat
    94381 accaatcagt gtagaaagaa ctaccatgta atgattctag aacactgata ttaataaaac
    94441 aagcaaaaat tcattttttt atgttttttt aactatctca gctatctatc ctctttatac
    94501 cattattttt ttcaaatagt gacctgtata actttctttt acgttaatga tttcttctgg
    94561 tgttccagtc gcaaggatcg ttcctccacc ttctccacct tctggtccta aatcaatgat
    94621 ataatctgct gttttgatta catctagatt gtgttcaatc actaaaactg tgttaccagc
    94681 atctactaat ctttgtaata catgcaacaa gcgggcgatg tcatcgctat gaagtcctgt
    94741 cgttggttca tctagtatat agaaattctt tccattagag attttgtgca attcactggc
    94801 aagtttcatc cgttgtgcct caccaccgga caatgtcgtt gctggttgcc ccatcgtcac
    94861 ataacctaag ccaacatcaa caatcgtttg cagtttgcga tgaatctttg gaatgtgctt
    94921 gaagaattct acagcatctt ctactgtcat ttccaaaata tcagcaatgc tttttccttt
    94981 gtaatgcact tctaatgttt cagagttata tcgtttgcca tgacatactt cacaaggaac
    95041 atagacatca ggcaaaaagt gcatttcgat cttaataatt ccatccccgc gacaagcttc
    95101 acaacgaccg ccttttacgt tgaaactaaa acgccctttc ttataacccc gcatttttgc
    95161 ctcgttcgtt tgagcaaata acccacggat atcatcaaat acacttgtat aagtcgctgg
    95221 attacttctc ggcgtccggc cgattgggct ttgatcgata tcgatgatct tttcgatact
    95281 ttcgtagccg gaaattgtct tgaatttacc tggcttagca gaattcttat ttaatttttg
    95341 cgctaacgat ttctttaaga tactattgac caatgtactc tttccagaac cagacacacc
    95401 tgtgactgct acaaattctc ctaatggaaa ttcaacacta atatttttca gattgttttc
    95461 tgaagcacca gtgatcttga ttgctttccc attaccttta cggcgttctt ttggtacagg
    95521 gattgatttt ttccctgaaa gatatttccc tgtcaaagaa ttttcatcat tagctacttc
    95581 ttcaggagtg cctgcagcca cgatttgtcc gccttgttct ccggccccag gtccgacgtc
    95641 aatcagataa tctgctgccc acatcgtatc ttcatcatgt tcgactacaa cgagggtgtt
    95701 gccaagatca cgcattttct ttaatgagcc aatcagtcga tcgttgtcgc gttgatgcaa
    95761 tccgatcgat ggttcatcta aaatatacaa aactccagaa agattcgatc caatttgtgt
    95821 agccaatctg attcgctgcg cttctccccc agataatgtt ccggaagcgc gactcaaagt
    95881 tagatagtct agtcccacat tttccaagaa tgaaagtctg tcatttactt cttttaaaat
    95941 cggtttggca attactttct cttgttcaga taacgttaag tttgagaaga aatctactgc
    96001 tttttgaata gacaactcat ttgtttgccc gatattcgtt ccattgattt gaacagatag
    96061 agcttgggga ttcaatcgat aacctttaca tgtctgacaa gttaattctg tcatataaag
    96121 acgcatttgc tcacgcgtga aatcactgtt cgtatcatga taacgacgct cgatattctt
    96181 caatacccct tcgaaagtcg tttctacatc gcgaacgcca ccaaaatcat tttcataatg
    96241 gaaatggaaa agatcatctg tcccattcaa gatgatatcc tgatgctctt ccggtaattc
    96301 ttcgaatggt gtatctaaat cgataccgaa actttcacaa gcctgcgcta acatttgagg
    96361 ataatactga gagctgattg gattccatgg aacgatagca ccttgactaa gtgtcttcgt
    96421 tctatctgga atcactaaat cttgatcgac ttctaatttg acacctaaac catcacagtc
    96481 tggacaagct ccaaaaggcg cattgaatga gaacagtctt ggttctagtt cgccaacagt
    96541 aaatccgcaa taaggacagg catagtgttc gctaaacaac atttcttctt caccgatcac
    96601 gtcaaccaga gcatagccgt ctgccaatcg cagagccgcc tctaaagaat caaatagacg
    96661 agaacgaatc ccttctttca caatgatccg gtcgatgacg atcgcgatat catgtttttt
    96721 gtttttctcc agctcaggca cttcacttaa atcatagatc tcaccatcga ctcgaacgcg
    96781 gacgtatccc tctttttgga ttcgttcgaa tattttttta tgttgtcctt ttttctttac
    96841 cacgatcggt gccaacactt ggatctttgt gcgctcaggc aaagccatta cttgatctgc
    96901 catttgatct actgattgac tggtaatctc gatatgatcg ttgggacaaa tcggatgtcc
    96961 tacacgtgca aacaacaagc gtaaatagtc attgatttcc gtcacagtac ctactgtcga
    97021 ccgaggattt ttacttgttg ttttctgatc aatagaaatc gccggactca atccgtcgat
    97081 gctatctacg tccggcttat ccatttgtcc taaaaactga cgagcataag cagaaaggct
    97141 ctctacgtat ctccgctgcc cttcagcgta cagcgtatca aatgctaggg aactttttcc
    97201 agaaccggac aaacctgtaa cgaccacgaa tttatctcgt ggaatcgtta catctatatt
    97261 tttcaagtta tgggcacgtg caccgtgaat cgtaatttta tcgttagcca tgttgtcctc
    97321 ctactttgaa gcttttaatt ctaaaatcat atctcgcaca ttagcggctg tttcaaaatc
    97381 caatgctttc gccgcatttt tcatttcttt ttctaatttc atcaataacg tatctttttc
    97441 ttctttcgtc atttcttcat acgagacttg taccgcttct tttgtatcat cttccgattc
    97501 tttcgtaata gaaatcaaat ctcggatttc tttaatgatc gtttttggaa cgatcccatg
    97561 ttcttcattg tacttctgct gaatcgtacg gcgtcttgat gtttcatcca tcgctagacg
    97621 catagaatct gttactttgt ctgcgtacat gattacttta ccttcttcat tccgagcagc
    97681 tcgtccaatc gtttggacta aggaacgttc actacgtaaa aagccttctt tatccgcatc
    97741 caagatagca acaagggaaa cttcaggtac gtctaatcct tcacgaagca aattgattcc
    97801 aactaacaca tcaaattcac ctaatcgaag atctcgaatg atttctgttc gttccagcgt
    97861 tttgatatca ctatgcaaat atttgacttt gatccctaat tctttgaaat aatctgtgag
    97921 gtcttctgcc atttttttcg tcaatgtcgt tacaaacacc cgctggtctt tttcgactcg
    97981 ttcatttatt tcgcctacta gatcatcgat ttgtcccatg attggacgaa cttcgatcac
    98041 tgggtcaagc agccctgtcg gacggatgat ttgttggata acggtatctg tttgttcatg
    98101 ttcatatggt cctggcgtcg cagagacata aatgatctga ttgacatgtt tttcaaactc
    98161 ttctaaccga agtggccgat tgtctaaagc agatggcaaa cggaaaccgt aatctactag
    98221 catctgtttt ctcgctcggt caccattata cattcctctg acttgaggca tggttacgtg
    98281 cgattcgtcc gccacgatca aaaaatcttc aggaaagaaa tccaatagcg tataaggcgg
    98341 ttcaccttct ttacgtccgt ccatatgccg agaataattt tcaattcccg acgtgtagcc
    98401 catttctaac atcatttcaa tatcgtaatt ggttcgctgt tctaaacgtt gagcttccag
    98461 aagtttgttt tcatttcgca agacagttaa acgctgttct aattccgctt tgatattcgc
    98521 tacggcatgc tccatatgtt catcattcgt cacaaagtga gtcgccggga agattgccac
    98581 gtgttccgtt tctcctaaaa cttcacctgt cagtgcattg acttctcgga tacgttcgac
    98641 ctcatctcca aaaaactcca cacgtaaggc acgttcatct cttgaagccg gaaaaatctc
    98701 cacgacatca ccgcgcactc gaaaacgacc gcgctgaaaa tctatatcat ttctttcgaa
    98761 ttggatactg actaagtcac gaatcaactg attgcgatct agttctgctc cctgtcgaat
    98821 cgaaactact tgtttttggt actcaaaagg cgaacctaat ccaaaaatac atgaaacgga
    98881 tgcaatgacg atcacatcat ttctttctaa taatgaacta gtagccgaat gtcgtaattt
    98941 gtcgatctca tcatttacac ttgaatcttt ttcgatatac gtatcgcttg aaggcacata
    99001 agcttcaggc tgataataat cataataaga aacaaaatat tcaacagcat tgttagggaa
    99061 aaattccttg aattccccgt acagctgacc agccaatgtt ttgttatggg caataattag
    99121 tgttggttta ttcactttct caataagatt ggaaatcgta tatgttttcc ctgttccagt
    99181 cgcacctaat aagatttgtg cttttttccc gccgactacg ccatccacta attggttaat
    99241 cgcttcaggc tgatcaccag ccggttggta tttcgagaca agctcaaagt gtcgggaggt
    99301 atctctctca atcattcttc gtcctccttt tcattcattc cttttcattt tacgaccaat
    99361 aaaaagaaaa ggaaagcaaa ggcattcctt actaaaaaat tataccacac cgaacatact
    99421 ttcgccactt ttttatctta ttatgcatgt tacgttttat aacataaaat gttagaaact
    99481 cgcatttttt tcattttttc gagcttacag tcttgtctga ttcagcactt ttcgcctata
    99541 ataataaaat attggaaatt taggaggttg attatgaaaa gaaagcattt atttcactca
    99601 tttgttttct taatgacagg acttgtgaca cttgcgtttg gacatgttgc acaagctgac
    99661 aacactacaa cggcagagtc aagcacaagc gagacaatgc aaaaaataga gcccaaaaaa
    99721 gacgtatacg taattgccag tgactctgct tttgctccat ttgaatttca aaacgcaaag
    99781 ggcgagtacg aaggcatcga tgtaaaattg atggaaaaca tcgccaaact gcaaggattt
    99841 aacatccgaa tggactaccg tggtttctct gctgctttgc aagctttgga atcaggacaa
    99901 gcagacggaa tgattgccgg aatgactgta acagatgaac gaaagaaaag ctatgatttt
    99961 tctgattctt actttgatag cgggatccaa ttagctgtca aaaaaggaga tacttcgatc
   100021 aagtcttatg aagatttgaa aggaaaaaca gtcggagcaa aagttggtac cgaaagtgct
   100081 gaatttttaa gcgaccatga atctgaatat ggctattcta tcaaatattt tgatgctgct
   100141 gatcaattgt acgacgggtt aaaagttggc gcaatcgatg caatgatgga tgattatccg
   100201 gtaatcggtt atgcgatcaa acaaggacaa gaattggaaa caccgatcaa aaaagaatct
   100261 ggcggaaaat atgcatttgc cgttaaaaaa ggacaaaatc ctgagcttct taatatgttc
   100321 aatgaaggat tgaaagaatt aaaaagaaca ggcgaatacg ataagattat taaagaatat
   100381 gtagcggatg gttcagaaac ttccagtgag tcttccactg ctgatgaatc aacatttctt
   100441 ggacttatcc aaaacaacta tcaagcattg ttgaaaggac tttggagaac aattctccta
   100501 actttgatct cctttgcttt ggctatagta atcggtgtga tttttggatt attcagtgta
   100561 gcaccaatac gcgctcttcg cacaattgct agtatttatg tcgatatcat ccgaggaatt
   100621 ccaatgatgg ttcttgcgtt ctttattttc tttgggttgc ctggtatcgt tggatttacg
   100681 attcctgact tccttgctgg tgttatcacc ttgactctta acgcaagtgc ttatatcgca
   100741 gagattgttc gaggcgggat caacgccgtt cctgtcggac aaatggaagc ttccagaagt
   100801 ttaggtcttt cttataatcg gacaatgcaa aaaatcattt tgccgcaagc gatacgcatc
   100861 atgatcccat cattcgtcaa tcagttcgtt atctcattaa aagacacaac gatcatttcc
   100921 gcaatcggtg tagtcgaatt gctgcaaact ggtaagatca ttgttgcacg tacaacacaa
   100981 agtacgtatg tttacttgat cattgcgatc atgtacttga ttttgatcac agcattgaca
   101041 aaattagcaa aagttttaga aaagaaggtg aagtaagatg gcagaaaaaa tcttagttga
   101101 gcatttagtc aaaaaatacg gcgataacac agtattaaac gacatcaatg tgtccatcaa
   101161 cgaaggagat gtcgtctgta tcattggccc ttcaggttca ggaaaaagta catttttgcg
   101221 ttgcttgaat cgtttagaag aaccttctga cggtgaaatt attattgacg gcgcccactt
   101281 gatggataaa agcacagata tcaacttggt gagacaacat atcggaatgg tattccagca
   101341 ctttaaccta ttccctcatc tttctatttt ggaaaacatc attttagcac cgatggacct
   101401 gaagaaagaa tctagagaag aagcagagaa aaaagcaatc aaattgctgc agacagttgg
   101461 tctagaagat aaaaaagata tgtatcctga aatgctttcc ggtggacaaa agcagcgtgt
   101521 ggctattgct cgtgctctag cgatgaaccc tgacatcatg ctttttgatg agcctacatc
   101581 tgcactcgac cccgaaatgg tcggtgatgt cttgaacgtc atgaaaaagt tagcagaaca
   101641 aggtatgacg atggttatcg ttacgcacga aatgggcttc gctaaagaag tggccaatcg
   101701 tgtcatgttc attgacgacg gaaacttttt agaagacgga acgcctcaac aaatctttga
   101761 aaatccgcaa aatgaacgga caaaagattt cttgaataaa gtattaaata tttaataaaa
   101821 accaaaaatg cccggtcgct tcatgacgac cgggcatttt tagtttcttg ttttttcact
   101881 taactgtacc agccgcatcg cttgttctcc tgcacaaaca agcaatcgct ctacctgttc
   101941 catcgcataa gaaagtacca tcggttcatt gacgatatca agtacatagt cgataccacc
   102001 ttcagaaact atgatgatta tcttattcta aagctggctt ttttgttggt acaaacatga
   102061 caactacaaa aaa
//
LOCUS       DAYYGS010000002        79365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000002 DAYYGS010000000
VERSION     DAYYGS010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 79365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.002"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..635
                     /locus_tag="ACHA44_000095"
     CDS             3..635
                     /locus_tag="ACHA44_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903424.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(769..894)
                     /locus_tag="ACHA44_000096"
     CDS             complement(769..894)
                     /locus_tag="ACHA44_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFM8903425.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(1028..1921)
                     /locus_tag="ACHA44_000097"
     CDS             complement(1028..1921)
                     /locus_tag="ACHA44_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HFM8903426.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2103..3029)
                     /locus_tag="ACHA44_000098"
     CDS             complement(2103..3029)
                     /locus_tag="ACHA44_000098"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HFM8903427.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3114..3875)
                     /gene="pflA"
                     /locus_tag="ACHA44_000099"
     CDS             complement(3114..3875)
                     /gene="pflA"
                     /locus_tag="ACHA44_000099"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HFM8903428.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4119..6350)
                     /gene="pflB"
                     /locus_tag="ACHA44_000100"
     CDS             complement(4119..6350)
                     /gene="pflB"
                     /locus_tag="ACHA44_000100"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFM8903429.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6627..9077)
                     /gene="parC"
                     /locus_tag="ACHA44_000101"
     CDS             complement(6627..9077)
                     /gene="parC"
                     /locus_tag="ACHA44_000101"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFM8903430.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9089..11134)
                     /gene="parE"
                     /locus_tag="ACHA44_000102"
     CDS             complement(9089..11134)
                     /gene="parE"
                     /locus_tag="ACHA44_000102"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFM8903431.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11300..11734)
                     /locus_tag="ACHA44_000103"
     CDS             complement(11300..11734)
                     /locus_tag="ACHA44_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFM8903432.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11861..12499
                     /gene="plsY"
                     /locus_tag="ACHA44_000104"
     CDS             11861..12499
                     /gene="plsY"
                     /locus_tag="ACHA44_000104"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFM8903433.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12631..13506)
                     /locus_tag="ACHA44_000105"
     CDS             complement(12631..13506)
                     /locus_tag="ACHA44_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFM8903434.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13588..14379)
                     /gene="codY"
                     /locus_tag="ACHA44_000106"
     CDS             complement(13588..14379)
                     /gene="codY"
                     /locus_tag="ACHA44_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HFM8903435.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14397..15797)
                     /gene="hslU"
                     /locus_tag="ACHA44_000107"
     CDS             complement(14397..15797)
                     /gene="hslU"
                     /locus_tag="ACHA44_000107"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HFM8903436.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15811..16359)
                     /gene="hslV"
                     /locus_tag="ACHA44_000108"
     CDS             complement(15811..16359)
                     /gene="hslV"
                     /locus_tag="ACHA44_000108"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslVU peptidase proteolytic subunit"
                     /protein_id="HFM8903437.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16396..17301)
                     /gene="xerC"
                     /locus_tag="ACHA44_000109"
     CDS             complement(16396..17301)
                     /gene="xerC"
                     /locus_tag="ACHA44_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFM8903438.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17499..18875)
                     /gene="trmFO"
                     /locus_tag="ACHA44_000110"
     CDS             complement(17499..18875)
                     /gene="trmFO"
                     /locus_tag="ACHA44_000110"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047151 - tRNA
                     (uracil(54)-C5)-methyltransferase activity,
                     5,10-methylenetetrahydrofolate-dependent [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HFM8903439.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18844..20922)
                     /gene="topA"
                     /locus_tag="ACHA44_000111"
     CDS             complement(18844..20922)
                     /gene="topA"
                     /locus_tag="ACHA44_000111"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFM8903440.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(21044..21901)
                     /gene="dprA"
                     /locus_tag="ACHA44_000112"
     CDS             complement(21044..21901)
                     /gene="dprA"
                     /locus_tag="ACHA44_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFM8903441.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21957..22724)
                     /locus_tag="ACHA44_000113"
     CDS             complement(21957..22724)
                     /locus_tag="ACHA44_000113"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFM8903442.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22717..23577)
                     /gene="ylqF"
                     /locus_tag="ACHA44_000114"
     CDS             complement(22717..23577)
                     /gene="ylqF"
                     /locus_tag="ACHA44_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFM8903443.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23837..24235
                     /locus_tag="ACHA44_000115"
     CDS             23837..24235
                     /locus_tag="ACHA44_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8903444.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24472..25014
                     /locus_tag="ACHA44_000116"
     CDS             24472..25014
                     /locus_tag="ACHA44_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8903445.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(25097..25648)
                     /gene="lepB"
                     /locus_tag="ACHA44_000117"
     CDS             complement(25097..25648)
                     /gene="lepB"
                     /locus_tag="ACHA44_000117"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8903446.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25800..26027)
                     /locus_tag="ACHA44_000118"
     CDS             complement(25800..26027)
                     /locus_tag="ACHA44_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HFM8903447.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(26024..26542)
                     /gene="msrA"
                     /locus_tag="ACHA44_000119"
     CDS             complement(26024..26542)
                     /gene="msrA"
                     /locus_tag="ACHA44_000119"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8903448.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26562..27275)
                     /locus_tag="ACHA44_000120"
     CDS             complement(26562..27275)
                     /locus_tag="ACHA44_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HFM8903449.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27278..28174)
                     /locus_tag="ACHA44_000121"
     CDS             complement(27278..28174)
                     /locus_tag="ACHA44_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8903450.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28332..29174)
                     /locus_tag="ACHA44_000122"
     CDS             complement(28332..29174)
                     /locus_tag="ACHA44_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8903451.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29306..29959
                     /locus_tag="ACHA44_000123"
     CDS             29306..29959
                     /locus_tag="ACHA44_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFM8903452.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(30022..30549)
                     /locus_tag="ACHA44_000124"
     CDS             complement(30022..30549)
                     /locus_tag="ACHA44_000124"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8903453.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30569..31516)
                     /locus_tag="ACHA44_000125"
     CDS             complement(30569..31516)
                     /locus_tag="ACHA44_000125"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFM8903454.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKRPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31528..33411)
                     /locus_tag="ACHA44_000126"
     CDS             complement(31528..33411)
                     /locus_tag="ACHA44_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8903455.1"
                     /translation="MKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKTS
                     LLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVRA
                     YEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIST
                     LSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDRY
                     FLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMRA
                     GVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRINDQ
                     TLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQE
                     NEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEKR
                     RLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVAD
                     KLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKEW
                     ETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSEW
                     VED"
     gene            complement(33574..35652)
                     /locus_tag="ACHA44_000127"
     CDS             complement(33574..35652)
                     /locus_tag="ACHA44_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8903456.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(35665..35940)
                     /locus_tag="ACHA44_000128"
     CDS             complement(35665..35940)
                     /locus_tag="ACHA44_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903457.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(36302..36757)
                     /locus_tag="ACHA44_000129"
     CDS             complement(36302..36757)
                     /locus_tag="ACHA44_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903458.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(36911..38119)
                     /locus_tag="ACHA44_000130"
     CDS             complement(36911..38119)
                     /locus_tag="ACHA44_000130"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HFM8903459.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(38231..38563)
                     /locus_tag="ACHA44_000131"
     CDS             complement(38231..38563)
                     /locus_tag="ACHA44_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8903460.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            38711..39586
                     /locus_tag="ACHA44_000132"
     CDS             38711..39586
                     /locus_tag="ACHA44_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8903461.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(39668..40249)
                     /locus_tag="ACHA44_000133"
     CDS             complement(39668..40249)
                     /locus_tag="ACHA44_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ReoY family proteolytic degradation factor"
                     /protein_id="HFM8903462.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(40255..41514)
                     /locus_tag="ACHA44_000134"
     CDS             complement(40255..41514)
                     /locus_tag="ACHA44_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8903463.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(41516..42562)
                     /locus_tag="ACHA44_000135"
     CDS             complement(41516..42562)
                     /locus_tag="ACHA44_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903464.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(42797..43072)
                     /locus_tag="ACHA44_000136"
     CDS             complement(42797..43072)
                     /locus_tag="ACHA44_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFM8903465.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(43315..44625)
                     /gene="der"
                     /locus_tag="ACHA44_000137"
     CDS             complement(43315..44625)
                     /gene="der"
                     /locus_tag="ACHA44_000137"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFM8903466.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(44807..46036)
                     /gene="rpsA"
                     /locus_tag="ACHA44_000138"
     CDS             complement(44807..46036)
                     /gene="rpsA"
                     /locus_tag="ACHA44_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFM8903467.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(46171..46851)
                     /gene="cmk"
                     /locus_tag="ACHA44_000139"
     CDS             complement(46171..46851)
                     /gene="cmk"
                     /locus_tag="ACHA44_000139"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFM8903468.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREKLVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(46900..47535)
                     /locus_tag="ACHA44_000140"
     CDS             complement(46900..47535)
                     /locus_tag="ACHA44_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HFM8903469.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(47601..49004)
                     /locus_tag="ACHA44_000141"
     CDS             complement(47601..49004)
                     /locus_tag="ACHA44_000141"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFM8903470.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(48991..50022)
                     /locus_tag="ACHA44_000142"
     CDS             complement(48991..50022)
                     /locus_tag="ACHA44_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903471.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            50053..50274
                     /locus_tag="ACHA44_000143"
     CDS             50053..50274
                     /locus_tag="ACHA44_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HFM8903472.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(50439..51176)
                     /locus_tag="ACHA44_000144"
     CDS             complement(50439..51176)
                     /locus_tag="ACHA44_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HFM8903473.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(51329..52669)
                     /locus_tag="ACHA44_000145"
     CDS             complement(51329..52669)
                     /locus_tag="ACHA44_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8903474.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(52906..54429)
                     /locus_tag="ACHA44_000146"
     CDS             complement(52906..54429)
                     /locus_tag="ACHA44_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFM8903475.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(54807..55418)
                     /locus_tag="ACHA44_000147"
     CDS             complement(54807..55418)
                     /locus_tag="ACHA44_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8903476.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(55541..55666)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(55748..56464)
                     /locus_tag="ACHA44_000148"
     CDS             complement(55748..56464)
                     /locus_tag="ACHA44_000148"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8903477.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(56470..57039)
                     /gene="scpB"
                     /locus_tag="ACHA44_000149"
     CDS             complement(56470..57039)
                     /gene="scpB"
                     /locus_tag="ACHA44_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFM8903478.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(57023..57793)
                     /locus_tag="ACHA44_000150"
     CDS             complement(57023..57793)
                     /locus_tag="ACHA44_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HFM8903479.1"
                     /translation="MNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDIPIATITEQY
                     MNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEEDPREELVTQ
                     LLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKKIDLIRAMQK
                     ILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESPSKSEVVTTF
                     MALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(57790..58161)
                     /locus_tag="ACHA44_000151"
     CDS             complement(57790..58161)
                     /locus_tag="ACHA44_000151"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903480.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(58180..59067)
                     /gene="xerD"
                     /locus_tag="ACHA44_000152"
     CDS             complement(58180..59067)
                     /gene="xerD"
                     /locus_tag="ACHA44_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFM8903481.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(59163..59636)
                     /locus_tag="ACHA44_000153"
     CDS             complement(59163..59636)
                     /locus_tag="ACHA44_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8903482.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(59741..60604)
                     /locus_tag="ACHA44_000154"
     CDS             complement(59741..60604)
                     /locus_tag="ACHA44_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor B family protein"
                     /protein_id="HFM8903483.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(60800..62899)
                     /locus_tag="ACHA44_000155"
     CDS             complement(60800..62899)
                     /locus_tag="ACHA44_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division site-positioning protein MapZ
                     family protein"
                     /protein_id="HFM8903484.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(63119..64228)
                     /gene="rpoD"
                     /locus_tag="ACHA44_000156"
     CDS             complement(63119..64228)
                     /gene="rpoD"
                     /locus_tag="ACHA44_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFM8903485.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQERVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHSSRSKQLKDFLE"
     gene            complement(64252..66093)
                     /gene="dnaG"
                     /locus_tag="ACHA44_000157"
     CDS             complement(64252..66093)
                     /gene="dnaG"
                     /locus_tag="ACHA44_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFM8903486.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(66275..68590)
                     /locus_tag="ACHA44_000158"
     CDS             complement(66275..68590)
                     /locus_tag="ACHA44_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8903487.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(68775..69635)
                     /locus_tag="ACHA44_000159"
     CDS             complement(68775..69635)
                     /locus_tag="ACHA44_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HFM8903488.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(69694..70326)
                     /locus_tag="ACHA44_000160"
                     /pseudo
     CDS             complement(69694..70326)
                     /locus_tag="ACHA44_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            70493..71254
                     /locus_tag="ACHA44_000161"
     CDS             70493..71254
                     /locus_tag="ACHA44_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8903489.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            71280..73871
                     /gene="mprF"
                     /locus_tag="ACHA44_000162"
     CDS             71280..73871
                     /gene="mprF"
                     /locus_tag="ACHA44_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8903490.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(74059..75246)
                     /gene="tuf"
                     /locus_tag="ACHA44_000163"
     CDS             complement(74059..75246)
                     /gene="tuf"
                     /locus_tag="ACHA44_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8903491.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            75614..75970
                     /locus_tag="ACHA44_000164"
     CDS             75614..75970
                     /locus_tag="ACHA44_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8903492.1"
                     /translation="MKKLIGVLVVLGIFLGESFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(76211..77215)
                     /locus_tag="ACHA44_000165"
     CDS             complement(76211..77215)
                     /locus_tag="ACHA44_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFM8903493.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            77381..78232
                     /locus_tag="ACHA44_000166"
     CDS             77381..78232
                     /locus_tag="ACHA44_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8903494.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            78249..78494
                     /locus_tag="ACHA44_000167"
     CDS             78249..78494
                     /locus_tag="ACHA44_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903495.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            78681..>79365
                     /locus_tag="ACHA44_000168"
     CDS             78681..>79365
                     /locus_tag="ACHA44_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903496.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAA"
BASE COUNT        21824 a        16672 c        13639 g        27230 t
ORIGIN      
        1 ttatggaaat tcaacgtaga accactaatt ttttacattt aggctctttt tttgcaactt
       61 tgacgatctt attttttcta tgttattcat accttaagac ctcacatgcg ccttctagtg
      121 cattaggctg gttgcttttg gatcattctg atatcacaga agccgaagaa aatccgtttt
      181 ttttgatttt gtcttctctt tgcggcttac tgttttctgt ttatttcggt gcctattttt
      241 ttcatcgaca tcctggtacg ttaaacgaag cttcaagcgt attgattgca tttaaaacgt
      301 tttctttttt agcaattctt ttttattcgt ttaatcacaa tacgcttttc ttctttatca
      361 tgaatggttt agtcgtgatt ttatccattt ttacttattt tattttttca ctcattttta
      421 gccaattgaa atgtccgctc ttctttcgat tagttcctgt cacctgtttt atttacagct
      481 tgttttcctt ttttgtcctt gctttaatgc cactgaccaa cccttcgacg atccaagtcg
      541 caggaaatct gttggatatg ctcttcatcg tcagtctagg tgtatttatg tggcaaagaa
      601 aaaacaaaca aaataaaaag cattttgaag cctgactatt acgacttcat aaatgctttt
      661 ttagttttaa acacttcaat ctaaaagaat aacccaagat tggataaatt attaatgact
      721 cgccattcgt ttcttagtga ttctggatta tcaaatggac ttcctaatct aatcagctag
      781 ttcttcccat aaaatgccat acttgactga atcataatat tgattattta aaaatctaac
      841 ttttcttatc actacttcct ttgtcattcc acatttctca ccattttttt gcattccttt
      901 gtttccagac catgtagtga atctaatagg tggtagatag gcaaaatact caaataattc
      961 ttttaaccat aaaggaagag aaaggctcct cctgcaagag tcagtataat ttttgcttac
     1021 tgctctctta ggttttactc actaaaactg ttccatattc tttgatataa gaaaatgcat
     1081 gcggtataaa tttcttgctc agctcagaaa agatttgtcg tttaagaaag gccatttcct
     1141 ccattgtcaa tgaaggatcc aatgttggta attcaatctc aaaattgttt ttttcgacaa
     1201 taggtaattc taatgtgaat gattcttctt cctttatagt attcgtcaca agtagagact
     1261 gatggaaaat actaggccgg cgatttttat atttattctt acgtatcctg tcaacgataa
     1321 cttctatttg atcaaaaaag tacgattcat ttacgtctcc aataagttcg gctaattctt
     1381 tgaatgcctt cgttacagga agctgattct tctgtacaaa aaagtaatta gccaaagtaa
     1441 gcgggaagtg ttctatagag gaaagtgtga taaaacggta cttctcattt cctgcagttg
     1501 tcctcagcaa ttctgtctct cggctgtttc ttactccata aaaatacgct tctttccgtt
     1561 catcttcgaa acaatacgcc caaataactt ctcccatcgc taatttttgt tcatcaacag
     1621 agagtctctt caattgttca gctatggttt cagcagccga agtggcttgt tgcaagtaat
     1681 ctttaggatc aaatattgga aagttcaaat gatatcttcg ttcttccctc ttgattaatc
     1741 ctgcatcgat caataaatca agaaaatggg aaaatttatt ttgcgaaaat tcttttttca
     1801 attctcttag aatcgtttct tgttctcgat gttcatatag ataatcggtc aatgaagaaa
     1861 aaagcggatc atcggtcagt ttttgataaa agttttcctt attttttgaa taatagttca
     1921 tatatgttct cttttctatc cgttttattt tacttaaaag gcttttacgt aaaaaggagg
     1981 aagaacaaga gtcataattg actgttattc tcctccattt cttcggcctt tagtatgttc
     2041 tatgtttctt tttatatcaa tcgcttatcc attcattttg aatgcttcat actaccattt
     2101 gattagctaa atacagccgt caattgaggt acgacttgtt ttttacgtga aacgacacct
     2161 ttaagcaaag cacggttgtt ggtcaaagtt gtttggaaag cttcttctac tttttctttt
     2221 cctttaccag caacaagtaa ttctgaatca ctgtctaaga tgtttgtgac aactaatacg
     2281 aacagatcgt agccatttgc tttcatttca tcttccattg ctgcttctag ctctgcttgt
     2341 cgatccatca cttcattaag gtccactgta ttgacttgag cgatacgaac gttggaactg
     2401 cccattggga aactttttgc atccaaatca agaagaaccg cagctgattt atcacttaga
     2461 tttgttcctg ctttcaacat ttcaagtcca taagtgttta gatcgacttc agcgattgct
     2521 gcaagttctt ttgcagctgc ttcatcttca ggcgtacatg ttggtgattt gaacaacaat
     2581 gtatctgaaa tgatagctga gagcatgatt cctgcgattt ctttaggaat tgttacttgg
     2641 ttttctttat atagttttaa aataatcgta cttgtacatc caactggttc tgcacgatag
     2701 tacaatggat tagctgtttc aaagtttgca atacgatggt ggtctactac agctaaaata
     2761 tttacttcgc tgatatctga aatactttgt tggaattcat tatgatccac aagcatgaca
     2821 tctgcatttt ctgctttagc tgactctact actcgtggtg cagatagctg aaagtggtct
     2881 aatgcgtatt gtgtttcttc attaggtgta cctagagcaa ctgcttctgt ttctttacct
     2941 aattcatttt gcaagtgtga aaacgcaatg gctgccccga ttgcatctgt atctggattt
     3001 tggtgaccaa aaactaaaat ttttgacatg tcattcgctc ctttgtgatt tctcctattt
     3061 aataatagca aaaaaacgga aagaagcaat acttatcttc tttccgatgc gacctatcgc
     3121 gttaaatatt ttgtatactc ttctgtatga agcaatcgtt tagcattttc gacacgttct
     3181 tttgtaggtg gttcgatccc ttctaatgga tatttcaaac caagtgtttc ccacttgtat
     3241 tttcccatcg tatgataagg aagaatctct actctgtcta catttgacaa ggtttgaata
     3301 aagttgtcta gtcggatcaa atattcatca taatcacttc gctcaggaac tagtacatgg
     3361 cgaatccaaa ccggtttgtt gatttttgac aaatattgag ccatttctaa aatattttca
     3421 ttgtcccaat gcgttagttt tttatgctct tcattatcga tgtgtttgat gtcaaataac
     3481 agtaaatctg tatacttcat caattcttga aaacgtgaaa agaaaggttc ttcatacgta
     3541 aaaggttttc cgcatgtatc caatgtagta tgcatcttcc gctcttttgc ttttttaaat
     3601 agttcgatca aaaaatcgat ctgcaataga ggttctccac cactaacagt aataccacct
     3661 ttatctcccc agtaaggtct gaagcgttct gctttatcta aaagttcatc tgctgtgtat
     3721 tctttccctc cgccaatatt ccaagtatct ggattgtgac aaaattggca acgcatgcgg
     3781 catccttgca taaacacgac aaagcgcagt ccaggtccgt ctactgaacc gaaagtttcg
     3841 atagaatgaa catagccgat tgttttttct tccatttaag ttcacatctt ttctttgatt
     3901 tcattttaag tacaagttag ctgtcgaagc gaagctttct gaacaaaatt tttcgcaatt
     3961 gccgcttcgt tatgtgtaaa atttacaggg acggaacata caagtcctgc ccctgtaaat
     4021 cgtttctttc aaataagcat actgattatt cgattttgat gcgatggcta ttcaacgtcc
     4081 tttcgacgct aagtcgaaaa acttttttca cgaacttttt acattctgtc gtgtgaagtt
     4141 ctcatcaaga catctaattg ttgttctctt gtcaagtcac ggaatttaac tgcataacca
     4201 gatacccgga tcgtcaagtt cggatatttc attggatttt ccattgcatc acgcaataaa
     4261 tcattactga atacattgac attcaaatga taagctcctt ttgagaagta tccatctaag
     4321 acattacgca agttttcgat tcttgtatct tgttcttttc caagacctga aggattgatt
     4381 gtttgggtat ttgaaattcc atccaatgca cagccgtaat tcattttagc tgttgagttc
     4441 aggctagcta ataacccact ttgttccgca ttgtaacttg gatttgctcc tggcgcaagt
     4501 ggttttccag cttcacggcc atcaggagta tttcctgtat ttttaccgta aacaacattt
     4561 gaagtaatcg tcaataatga agttgttggt tttgagttgc ggtaagtttt atgacgtttg
     4621 atttgtccca tgaagtattc caagatccat gaaactaatt catctgcacg gtcatcatca
     4681 tttccgtatt tagggaagtc gccatccact tcaaaatcaa ctgctattcc tttttcatta
     4741 cggattgctt ttacttttgc atatttgatc gctgacaagc tgtctgccgc atgagagatc
     4801 ccagcaatac ctgtagcaaa tgtgcgttgc aagtcagaat ccatcaaagc cagctgagct
     4861 gcttcataag aatatttatc atgcatataa tgaatgatgt tcaacgtatt gacatatagt
     4921 tctgctaacc aatccatcat attcatgaag ttatgtttca cttcttcaaa gtctagtatt
     4981 tctgatgtga ttggacgata ttctggtcca acttgggcgc cagtcacttc atcgactccg
     5041 ccattgatgg catataatag tgttttagcc aagttagcac gagcgccaaa gaattgcatg
     5101 tcttttccga caactgttcc tgatacacag caagcaatcg cacaatcatc agaaccccat
     5161 tgttcacgaa gctgattatc attttcgaat tgaatcgaag aactttcgat agcgattctg
     5221 ctagcatatg ttctgaatcc ttctggaagg tgttcagaat acaaaacagt caagtttggt
     5281 tctggtgaag gtcccatatt tgttaatgta tgaagcagac ggaaatcatt ctttgtaact
     5341 aatgaacgac catctaatcc catacctgca atggataaag tagcccagat agggaatcct
     5401 gagaataatt ggttatattc aggtgtacgc gcgaatttga ccatacgtaa tttcattact
     5461 aagtggtcga tcaattcttg tgcttctttt tctgtgatta ctccattatc caaatcacgt
     5521 tggatataga tatcaaggaa tgctgagata cgtccgattg acatcgcagc accattttgt
     5581 gatttgattg ctgctaaata tccgaagtat agccattgga cagcttcttt tgcatttgca
     5641 gctggtttag aaatatcata gccatatttc gcagccattt ctttcatttg cgctaatgca
     5701 cgatattgtt cagtgatttc ttcacgtaaa cgaatcactt cttcactcat tgtatgataa
     5761 ccagtcatat catgatcttt tttcttttgt tccatcaaat agtcgattcc atacaatgcc
     5821 acacgacgat agtcaccgat gatacgtccg cgaccgtaag cgtctggaag ccctgtaatg
     5881 attttgtttt tacgagcagc acgcatttca gcagtataag cgtcaaatac accttgatta
     5941 tgtgttttgc gccagtcagt gaaaatatgt gtcaattcat cgtttggtgt atagccattg
     6001 ctttttagcg cattgttagc catattgatt ccgccgaacg gcatgaatgc ttgtttcaaa
     6061 ggtttgtctg tctgtagacc gacgattttt tccaaatctt tatccaaata gcctgcatcg
     6121 tgtgaagtaa tagttgatac gatttctgtg tccatatcat agacaccgtt acgctcgaat
     6181 tggatatcat ttaacttttg caattcatcc cataattttt ctgttgcttc tgttgggcct
     6241 tccaaaaagc tgtcatcacc gcgatattca gtgtagttat tttgaataaa gtctctagtg
     6301 tctacgcctt ctttccattt ggtaccttta aatccattcc attgctccat tgtatggacc
     6361 tcctaaattt ctgttttatg taacagtttt tacgtgatta gtataacaca gcaccctatc
     6421 tttgcaaggg ttttcttgtt ttttatttaa caatttttat gttttattta catatattta
     6481 aatcaacgtt gacataccaa catttaagca ttgtgaacac tttcatcttt ttttgcaaac
     6541 gtataaggat ttattaaaaa tgaatgaaaa agagacgcca cctgtattaa tacagatggc
     6601 gtctcttcat taaaatacta caacagttac gactcgatga aaatctcagg agcatcgata
     6661 acttcgatga cttctccacc ttttttctca tccatcgcaa atgatccatt cgatgttcga
     6721 tcacctatag ggaaatcagt tgggtcaatc gttaactgct gccctttttg attcatcaag
     6781 gttatttctt ttgatcttcc ttccgacata tagataatac ggtgcggatt ttttttcagc
     6841 tcacgcatga ccgtcactcc ccgcttcgca cgtgaagttt ggctgatttc ttgagcaagc
     6901 atgcgtttaa cagctcctcg ctgagtaacg attacgatcg gcgtatcacc ttctgctaac
     6961 acaagcagcc cgttgataat cctatctcca tctttcaaat tcatagactt cactccagct
     7021 gcctttgctc caacaacggg aacatctgtt aaaggatagc gcaaacccat tccttgatgc
     7081 gttactaaga aaacatctaa gcctggctga tcttctttca gatacacagc aatcagttca
     7141 tcttcttccg cctttagctt catacagctg agcggacggc tcttataggt gcgccatggt
     7201 tcgaactctg tcattcgtgt ttgcttgatc aatccattct tgctgatgaa tacaaatgtt
     7261 ttctctgctt gaattttttg atatgggaaa acagctaaaa tcgattcgtc tacagacaga
     7321 ttggagattg tttgagaaat gtgctcacca gcatctttcc atttcaaatc gggtagttca
     7381 tggacagggc ggtagatcac atttgcttta tttgtgatca gaagaacatg gtccaatgta
     7441 ctcaattcac ctgagtatag caagtagtcg ccttctcgca tcccgatttc ttctggtttg
     7501 gaagcactat aagaacgaag gcttgttcgc ttcacatagc cttctcgcgt cacactgaca
     7561 attacgtctt cttgtgccac taagacttgc gtatcaattt tgatttcttc gatttcgtct
     7621 tcgattgtcg ttagacgagc ggaagaataa ttttttttca cttctcgcaa ttcttttttc
     7681 ataacagaga aaagctcttt gtcatttgaa aggattttat tcaactctgt gatttgcgca
     7741 atcaagcttt cggattcctt ttgaagctct gtgatatccg tgttcgtcaa acggtataat
     7801 tgcaatgtga caattgcttc tgcttgttct tctgtaaatt gaaagacgtc gactaaattc
     7861 tttttggcgt cttttttgtc tttactttca cgaatcgttg cgattacttc atccagaatc
     7921 gataaagctt tcatcaatcc ttctacgata tgctgtcttt tttgagcttt ggctaaatcg
     7981 aattgactac gttttgttat gacttgcttt cgatgctcga tataacttct taaaatgtct
     8041 ttcagtccaa cttgatgcgg agtcatatgg tcgatagcga ccatattgaa attgtaattg
     8101 atctgtagct cagtgttttt gaacaaataa ttcaaaattc cttgagcatt ggcgtctttt
     8161 ttcaattcga cgacaatttg cagacctgtt ctatcacttt catctcgtac ttcagctatt
     8221 ccatcgattt tcttattcaa acggatctca tccatttttt tcaccaaggt cgctttattt
     8281 acttcatatg ggatttctgt gatcacgatt tgctgtttat tacccttcat tgcctctatc
     8341 tttgttttcg aacgaagaat aacttttcct cgtcctgttt cataagcttt tttgatttca
     8401 tcttttcctt gcaagatccc tcctgttgga aagtcaggtc caggaatata ttccatcaat
     8461 ttttcaaggc tcgcttgtgg atgatcgatc atatagattg tgccatcgat cacttcagct
     8521 aagttatgag tcgggatttc tgttgcatat ccagcagata tacctgtcga tccattgacg
     8581 agaaggtttg ggtatcgagc tggaaggact gttggttctt tttctgtatc atcaaaattc
     8641 cacacgagat cgactgtttc tttctcgata tctgctagca tctctccact taacttggat
     8701 aatctggctt cagtataacg catcgcagca ggtggatcac cgtccatact cccgttattc
     8761 ccgtgcattt cgatcagtac ttcccgcaac ttccagtctt ggctcatgcg gaccatcgct
     8821 tcataaatac tgatgtcacc atgaggatga taattcccca tgatatttcc gacagatttt
     8881 gctgatttac ggaatccttt gtcatacgta ttgccatctt tgttcatggc aaataatatc
     8941 cggcgttgca ctggcttcaa tccatcacgg atatcaggca gtgcacgttc ttggataata
     9001 tatttggaat aacgtccgaa gcggtcaccc atcacttctt ctaaagttaa ttcttgtact
     9061 tcttgtcgat tttccatcag gtgacctcct attctacttc aaataactga ttgttttcat
     9121 ttttatctgc tcgttcttca tctaataaat cattagaaac tgaaggagag atttcagtat
     9181 cctctttttt atctaagata ctgccatctt cttcgagcgt aaattgcacg tgattttcga
     9241 tccattttct tcgaggttct actttgtcac ccatcagtgt ggttactcga cgttcagctt
     9301 gcgcagcatc atcgatacgg acgcggatca acgtacggga agtcggatcc atcgttgttt
     9361 cccacaattg ttccgcattc atctctccaa gtcctttata tcgctgcagc atatatcctt
     9421 tgccgacttt tttaatcatt gctgccaatt cgtcatctgt ccacgcatat tcaatgactt
     9481 gcttctttcc ttgtcctttg gatactttat ataaaggcgg taacgcgata tatactttcc
     9541 cagcttcaat cagcggtttc atataacgat aaaagaaagt aagcagtaat acttgaatat
     9601 gggcgccgtc tgtatccgca tcggtcatga tgattacttt gtcgtaatta cagtcttcga
     9661 tagaaaactc aggaccgact cctgcaccaa tcgtatagat catcgtattg atttcttcat
     9721 ttttcaagat atcttgcatt ttagcttttt cggtattgat gacttttcct cgtaaaggta
     9781 gaattgcttg gaactttctg tctcttcctt gtttagcaga tccgccagca gaatcccctt
     9841 cgactaaata caattcattt ttcttagggt ttcgggattg tgcgggtgtc aattttcctg
     9901 aaagaagaga ttcgcctttt ttacgcttct tcccatttcg gctttcttct ctggctttac
     9961 gtgctgcttc tctcgcttct ctggctttga ttgctttacg gaccaacatc tggctcattt
    10021 cgctattttc ctgcaaataa aagcccattt gttcactgat gacgttatct actacggtac
    10081 gagcaactgg cgtaccaagt ttttcttttg tttgcccttc aaattgcaga agattttcag
    10141 gtacacggat agaaagtaca gcagctaacc cttctctgaa atcactacct tccagattct
    10201 tgtcccgttc tttcagcaaa ccaacttttc tcgcatattc attataagat ttcgtcatcg
    10261 ccgctttcat accagcttca tgggttcccc catcttttgt ccgaacgtta ttgacaaaag
    10321 ataaaacatt ttctgaataa ccgtcattgt attgataagc gacctctact tcgatacctt
    10381 ctttttctcc tgaaaaataa acaactggtg tcaatgtatc tttttcttca ttcagataat
    10441 caacaaattc tttgattcct tcttcgtaat gaaaaatttc cttgactggt tcttctccac
    10501 gaagatcaga aagagagatt ttaacgcctt ttagaagaaa ggcagattct cttaaccgtt
    10561 cggctaaaat ttcatatgaa aatttcgttg tactgaatat tgtatcatct ggtaaaaaat
    10621 ggacagtcgt cccgtttgct ttttttgttt ttcctatctt tttcaaagta ccgtcaggtt
    10681 taccgccgtt tttaaatttc tgctgatact ctacgccatc tctaacgatc gttacaatta
    10741 gccatttcga taaggcattt accacactgg ctcctacacc gtgcagtcca ccagaagttt
    10801 tgtaccctcc ttgaccaaac tttccgccag catgcagcac agtaaaaatc acttcaactg
    10861 ttggtattcc ggaagcatgc attcctacag gcatcccacg tccgctatct gcaacagtta
    10921 tactattgtc ttcatggatg gtcacatcaa tctcactgcc ataacctgat aatgcttcgt
    10981 caactgcatt atcgacgatt tcatagacta aatgatgcaa gcctcggctg tcagtggatc
    11041 caatgtacat ccctggtctt tttctaactg cctctaatcc ttccaatact tggatggacg
    11101 agtcattgta ttcgttgttt actttttttg ccaaggcaaa cgctccttca ataatacttt
    11161 gcggcaaaac cgcaccacga ttatcatact agaaaaggtc gtgaaaaaaa agagggtaga
    11221 cgagaaatca attttaatta tctcctaacc gctttttcga cattcccctc tatcgatcat
    11281 ctcttctttg tggtcatttt tatttttggt tttctttcaa cttaccaagt tcaatcttaa
    11341 tacaacgatc cattacgata tctgtacgtc ctgcttttct tagcatctca gctgcttctt
    11401 tgttttcaat ccctaattgt gcccagaaca ctttagcatc agttttcaaa aaatcacctg
    11461 ctatttcagg taaaaattca ctccgtctaa atacatctac gatatcgatt ggatcatcga
    11521 tttctgtcaa attcgcatag actttctcgc ctagaatttc ttcaccagcc aataatggat
    11581 ttactggtat gattttatat ccattgtctt gcaatacctg tgcaaccatg taacttgttc
    11641 gttctggttt attgctcaag cctacgacgg caatattttt tgcttcttcc aaatagttga
    11701 aaatcgtttc ttgtgttgga ttttttacag acatttctgc caccccttgc ttttcttttt
    11761 attccatcta ttatacttgt atctttcatt ttttcaattg ttcctgttac ctattcatgc
    11821 taaaataaag gatagttttc aacttgaaag agggtaaaaa atgaaattga tcttattact
    11881 tattttagca tatcttttag gttctattcc ttccggtgtc tggattggca aattgttttt
    11941 caaaaaagat atccgacaac acgggagcgg taatactgga acgaccaata catttcgtgt
    12001 attaggcaaa acagccggga ttgttgtcct gcttatggat attttaaaag gtacattggc
    12061 tacttgtttg ccgatgatct tccacctttc tgggattaac cctctttggt ttggggtatg
    12121 cgccattctt ggccatacat ttccgatttt tgctgggttc aaaggaggaa aagctgttgc
    12181 gacaagtgca ggaatgcttt taggatttaa tcctgtattt tttgtttatt cttgtatcat
    12241 ctttatcgtt agtttatttt gtacaagtat ggtgagtttg agcagtatga tcagtgcagt
    12301 attgatcact ttatctacga tcgtccttcc ttatgtagct cctgtgattt tggctaagcc
    12361 caattggtta ctgacgatca ttgccttttt agtttcttcc tttatctttt atcggcataa
    12421 agacaatatc aagcgtatca gaaaccatac tgaaagtcga atcccgtttg gtttgaatag
    12481 cagcaagaaa aaatcctgat actttttcat tccaaagaga taaaacggga gtgtgacaaa
    12541 tcgatgtcac actcccttat tacttacagg atagcttttt tacacacctt ggtaaacggt
    12601 gttccctgct aacttctcgg tcacaacctc ttacttcact gtgatggaat attttgtctt
    12661 gaagatttca tgggcaccta gttgattgat ccctactttt tccatcaagt ttccactact
    12721 gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa acgaaaggcg cttcttttgg
    12781 ataaggagac cagatcccta cataaggcat atctttatag cttaaagtta cgctatggga
    12841 agatttttca cttcgaatgg cgtacgcatt tagacccctt gtttcaaaaa ccaaggcatc
    12901 ttgctcaaaa agttcacgat tcaaagccag attcgtattt gtctgaccta acgtcttttg
    12961 atccatatcg atgaatgggc ctttcaatgg taggcgtaat cttgatttca atggagaaaa
    13021 tgccagatag aaatcttcaa atgacaatcc ttcttcaaga ggtacattga aagcaggatg
    13081 tccaccgata gaaaagaaca tttctttttc accggtattt gccacttgat aatgaaccgt
    13141 gatgccgtct cctcctaatt catagctgat cactaattca aaatcaaatg gatacttttc
    13201 taatgtctcc ggcgtactcc ttaagatgaa actagccatt tcttgtccat gttcaatgac
    13261 ttcaaaatcc atatcccgag caaatccgtg ttgccccatt tgatatgttt tcccttgata
    13321 aacatactga tcatctttta aacgtccaac aaaagggaat aaaaccggtg catgtctgcc
    13381 ccaatacgct ggatcacctt gccagatata ctctaaacca tgttcttttg atttcagact
    13441 gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc tggtcatttt gaataataac
    13501 agtcattgcc attcctccaa cttcgactta ctttttaaaa tgggaaggct ggaacaaaca
    13561 tttctatctg ccccagtccc cttacttcta ataactgtgt tttgctaact cgtctttaaa
    13621 acgggaattc aatactttta aataagttcc cttcatgcct aacgaacgag attcaatgat
    13681 tccagctgat tccaattttc gtaaagcatt gacgatcaca gaacgagtga tgccgatact
    13741 atcggcaata ttagaagcag tcagacgacc ttcatctcca tccaatgctt caaagatagc
    13801 ttgtacagcc ttcaattcgc tataagataa tgtgttgatt gccatttgga cagccgttgc
    13861 actccgtaca tctgcttcga tgcttcgaga cttctgataa aggatctgca taccgacaac
    13921 cgtagctcca tattctgcta agactagatc gtcatctgca aacgattgtt ccatacgaga
    13981 taagataatc gtacccaaac gttcgccagc tccaaagatc ggaacgacag tcgtcagacc
    14041 aaatggatat ttctctcttg actctaccgg aaatgcagta atatcactag taattgggat
    14101 attagcttca gttttcatca actgtgccac tgtatcagta taagattcag gaaattgttt
    14161 ctcaacaaac attgttttga ttcgttcatt gttcacatcg tgacgttcat tataacctag
    14221 taatactccg tcacttccta tgatataggt gttgctgtca agaataccac ctagtgtgac
    14281 agccatttta tcgtaaggca aatctgcttg catgtcaaaa gtattctttt gctgcaaaag
    14341 tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt gtcgtcctcc gattttttat
    14401 aaaatatagc gacttaaatc ttcatttgcc gcaattgagc caagtttgtc atccacatat
    14461 gcttctgtga tcgttatttc tcccatttgc atatctggtg cttcaaataa tagatcttct
    14521 agtagttttt ccaaaatggt atgcagtctg cgtgcgccga tattatctgt gtcacggttg
    14581 acatcgaaag cgatttgagc gatccgttca attgcttctt ttgtaaacgt aactgacaca
    14641 ttttctgtac cgatcaacgc gacgtattgt tttactaaag cattatttgg ttcggtcaga
    14701 atcttcacaa aatcttcagc tgtcaaatca tctaattcta cccgtatcgg gaagcgtcct
    14761 tgaagttctg gaattagatc acttggttta gacaaatgga aagctcctga agcaatgaat
    14821 aggacgtgat ctgtttggat gatcccgtat tttgtgttta cttgtgaacc ttcgacaatt
    14881 ggcaatatgt ctctttggac gccttctctg gatacttctc cagattgctg tgatttagaa
    14941 gtgatcttat cgatttcatc gataaaaata attccacttt cttccgctaa tttgattgcc
    15001 tcactatgga tatcagcatc cttgactaat tttgctgatt cttccttgat caataactct
    15061 aaagcttctt tgactgttac cgtccgttcc acttttttct tcggtgtcaa agcacctaga
    15121 gtttcattta ggtcgatccc catttgctcc aatccattgt tcatcattgg tgcaggtttc
    15181 ttttggtctt caacttcaat ggttacttca cgactatcca gtaaaccctt ttgtagttgt
    15241 tccaagactg tacggcgatt gattcgaata tcttctgtca cttcttcttg cggttcagcg
    15301 gtattttgct gactagcatt aaaaagattc atcatttgtt caaattgatt agtggattgt
    15361 ttctgttctt ttttgatacc aggtaccaag atttttacca aacgtttttc tgcatttttt
    15421 ttagcacttg tgtatacacg gctgtactgc tctttttcta cgatttggat cgcattttct
    15481 actaaatccc gaaccattga ttctacatct cttccgacat aaccgacttc agtaaatttt
    15541 gttgcttcga ctttgacaaa tggtgcattg acgatttttg ccaaacgtcg tgcgatttct
    15601 gttttaccga cccctgtagg tccgatcatc agcatatttt ttggtgtaat atcttgttgc
    15661 attttttcat ctaactgcaa acggcgatag cggttacgca aagcaacggc aacagatttt
    15721 tttgctgcgt gctgtcctac gatatattca tctaattcac ttacgatttg tcttggtgtt
    15781 tgttttaatt catacatatc tactcacctc ttacagttct tctacgataa tattatgatt
    15841 agtaaagata cagatatctg cggcaatatt taacgcattt tctgcaattt ctttcgcact
    15901 catgtcgttt tgagcatagt ttttcattgc tcgggctgca gccaacgcaa aattccctcc
    15961 tgatccgata gctaagatac catcatctgg tgtgataact tctcctgtac ctgaaacaag
    16021 aagcatttct ttatcattca ttacgatcag catcgcttca agtttttgca tagattgctg
    16081 tgtccgccat tcttgagcta attcgacagc tgcgcgttgt agattcccgt tatattcgtt
    16141 tagtttgcct tcaaattttt cttctaaagt aaacgcatct gcaacacttc cggcgaaacc
    16201 tacaacaact tctccattgt agatacgtcg tactttcttt gcagtgcctt tcatgacaac
    16261 agattcaccc attgttactt ggccatctcc agccatagca aatttcccgt ctttttcgac
    16321 ggcacagata gtggttgaat gaaatggtga ttcgaccatg tttgtttcct cctaaatttg
    16381 ttcttttcct tatttttacg ctcttggatg gaaagaacga tagttttttt gtaaactttc
    16441 ttttgttaca tgggcataga tctgggtagt cgataaattg gcatgtccta gcaattcttg
    16501 gactgtccgc aaatctgccc cattatttaa taaatgagta gcaaatgtat ggcgcagcat
    16561 gtgaggatgg atatcactat ccaaactgct ttttttgatc atttgattca agacatattc
    16621 aattccagta ggcgtgattg gttttccatg atgattcaca aaaacgatat catgctcttc
    16681 gtgatatttt gtcatcaata cctggcggcc ttccctgaaa tattcttgca gagcatcttg
    16741 agcaaaagaa cctagaggag catatctctc tttgttccct ttaccatgga tcaacatgac
    16801 ctctgaatca aaatcaatgt ctgataccga caaactgcta cactcactta aacggatgcc
    16861 tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa tccaaaggac tatctcctgc
    16921 tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa aaacgtggca gtctcaaatt
    16981 tttctttttg agatgaacgt aagaaaatgg attttctttt atcacttctt ctttgagaag
    17041 atactgataa aacgaacgta ggctggctat cttgcgactg atagaatttc tgctatagct
    17101 aggacgatga tcagctaatg cactcaaata gacacgaacc gtaaaatatt ccgccttcag
    17161 cagatcagcg tctccagatt cctccaaaaa atgtttgaaa tggagaatgt cttcttcata
    17221 tgcttctttc gttttttctg aatatcctct ttcgacaatc aaataacgca aaaatttttg
    17281 ttcccacgtg gcttctgtca ttttctcacc ttccttttgt cccagagaat aatttagcac
    17341 agcgtttata aaaagacaaa taaattgtca gaatttaacc aatatttcta agtttgttca
    17401 caattggcgc aattcctcac attttgtttg agaaattctt aagaaaaacg caagaaaaag
    17461 agcgagacta tccgtctcac cctcttcatg gagtttcctt actcatttgg gattgcagtt
    17521 ttttctaatt ctgtttttat tgcctcatta gcttttaaag ctcgttgtgc aattgcttca
    17581 tatcttgctt ttttgtctcg aatgcgctct ggcagttcag gaaacagacc atagttggca
    17641 ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt agtaagccat acttccaata
    17701 gctgtttctt gtggaaactc gactggttcc atttcttttg ctaagcgtgc ggcgttgatt
    17761 ccagcaagta gtccgctcgc tgcactttca acatagcctt ctacaccagt catctgacct
    17821 gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg gtttcagcaa ttctggcgaa
    17881 ttcataaagc tgtttcggtg catcacacca tagcgaacga attcagcatt ttctaaccca
    17941 ggaatcatcc ggaaaacacg tttttgctcg ccccatttca agtgagtctg aaagcccacg
    18001 atgttgtata aagaagctgc agcattgtct tgacgcagct ggataacagc ataaggacgt
    18061 ttgccagttt taggatcttc caatccaact ggtttcatag gtccgaaaag catcgttttg
    18121 aatcctcgct gtgccattac ttcaattggc atacatcctt caaaatattt ttctttctca
    18181 aaagatttaa gttctgccac ttcggcattg attagtgcct catggaaagc tttgaattcc
    18241 tcttctgtca tcggacagtt caagtaagct gcttctcctt tgtcatagcg ggacttcaaa
    18301 tatactttat ccatatcgat cgtagctttg tctacgattg gtgcagctgc atcatagaaa
    18361 taaaaaccat ctgaaccatt gaatgctttg atgctttctg ctaatggatg agaagtcaaa
    18421 ggtcctgttg ctactacggt gattccttcc ggtatttcag taatttcttc gtttatcact
    18481 gttactaatg gatgctcttt gacttttcgg gtaattgttt cagaaaaaga atcgcggtca
    18541 acagctaagg caccaccagc tggcactgct gtttcatctg ccgaagtgat tactaccgaa
    18601 ttcaatcggc gcatttcttc tttcaataac cctactgcat tagccaaact gtttcctctt
    18661 aatgaattgg aacatactag ttcagcaaaa ttacttgtgt gatgcgcctc agtcgatttg
    18721 actggacgca tttcatataa acggacaggg actcctgctt gtgcgatctg ccaagcagct
    18781 tcacttccgg caagccctgc tccaacaaca tttaccattt tcaatgaaag aacctctttt
    18841 ctattatttt tggatgtctt cttcgtaatc tccatttaca cagactactt gtttaccgcc
    18901 tttgactttc ttctcgacca gatatccgtc acattttgga caatttctgc ccactggttt
    18961 atcccaagaa gtgaattcac attctgggaa acgggaacaa ccatagaata gtctgttttt
    19021 cttagatttt cgttcaatga cttgtccttc atgacatact gggcaagtca caccgatttc
    19081 ttttacgatt ggtttcgtat tccgacaatc aggaaaatta ctgcaagcat aaaacttccc
    19141 atatttccct aattttatca ccatcggatg tccacagaca tcacaatcga atcctgctgg
    19201 ttcatctttg atttgaattt tttcgatttt ttcttccgca ttcgtcagtt ctttttcaaa
    19261 cggtttatag aaacgatcaa ccactttgac ccacgcttcc gtgccttctt caattttatc
    19321 caaatctcct tccatttctg ctgtgaaatg gatatcgacg atttgaggga agaattcaca
    19381 gattaatgaa ttgacaattt ctcctagttc agtcggctca aatctttttt gtgtcaattt
    19441 tacatagtag cgtctttgga tcgtatcaag tgttggtgca taagtggaag gtcgccctac
    19501 cccgttttct tctagtgcac gaatcaacgt agcctcacta aaacgtgctg gtggttgagt
    19561 aaaatgttgt tttggttcaa tatcaacaga ttggactaca tcgccttctt caagttctgg
    19621 aagaatattt tctttctctt ctttgccgtc atctctacct tccacataca cttgcatgaa
    19681 tcctttgaat ttgatttttg aaccgttcgc aatgaagatg acaccatttt gttctaatgt
    19741 cactttcatt gtatctaaaa tagctggtgt catctgacta gccacaaaac gtgaccagat
    19801 caatgtataa agtttcaatt gatctttatc taaatattgt ttgagttcat ctggtgtacg
    19861 aagcacactc gttggacgca cagcttcatg ggcatcttgt gcgccttgag catttttgat
    19921 ctttcggtga tcatgtctag aaaattcttt tccatacgta tcttcaataa atgctgcggc
    19981 ctctgctttt gctgtatcgg caatccgggt agaatcagta cgcatgtatg taatcaagcc
    20041 gactgttcct tgtttaccca aagtgattcc ttcatataat tgctgtgcta ccatcatggt
    20101 ttttcttgtt cgaaagttta acttacgggc agcttcttgt tgcaggctac ttgtagtgaa
    20161 aggattcgca gggtttcttt tgcgctcttt cttttctact tttttaactt cgaagtctgt
    20221 tccatcaatt ctagcagtga cttcttttac actttgtgca tctggtaatt tctttttctt
    20281 accgtcgatt ccccagaaat ttgctttgaa tttcttgcga gcttttttaa aattaccgtc
    20341 gatgctccaa tattcttctg gaacaaattc tcggatctct ttttcacggt caataataat
    20401 ttttaaagcg acagattgga cacgtcctgc acttaaccct ttcttgacct tacgccagag
    20461 aataggactg atcgaataac caacgattct gtctaaggta cgacgagctt gctgtgcatc
    20521 tactaaatct acatcgatcg ttcttggttc cttaaaagct gctttgactg cgtcttttgt
    20581 gatttcattg aagaccaccc gatttttttc tttcagatcc aacccaagta ggtacgctaa
    20641 atgccaagca atcgcttctc cttctctatc cgggtcactt gccaagtaaa ctttttgagc
    20701 tttttttgct gcggctttca ggcttttgat gacatcgcct tttccgcgta tagaaatata
    20761 atggggttca taattgtttt caaaatcgat acccatttta cttttcggta aatctcttat
    20821 atggccaaca ctggccatta ctttatagtt tcgtccaaga tatttctcaa ttgtcttggc
    20881 ttttgcaggt gattctacaa tcactaaata tttatacgcc attactgaat ggactccttt
    20941 ccattgtcgt gttcctacta ttaaaagagg attcatacaa atcgtagcta atcatatcca
    21001 ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg gtgttattct ttaaattctg
    21061 ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc ttggatcaaa tgatgacatc
    21121 cggtagaatg ttctgttaaa atatttcctg gaacagaaaa aactgttctt ccaagatcca
    21181 atgcttgctg tgctgttatc agagagccgc tttttttctt ggcttcaaag acacatgttc
    21241 ctttagacaa agctgcgata atccgatttc tcattggaaa acaatgcttt ttcgggccgg
    21301 attgaggagg atattcactc aaaagaagat gatcatttgc aatcacctct tgcaagtcac
    21361 gattttcagc aggatagact tgctctatcc ctgttccaat gactgcgatt gtccgtccac
    21421 catgagagat tgctgcttgg tgaccgtatg tatctatccc tttcgcgcag ccactgacga
    21481 tcacatatcc agcttctatc actggtttta gtaaataata aactgttttt ttggctaaag
    21541 cagaagtgtg tcttccacca acaacgctta acatgttaca agaaagtaaa gatagatcac
    21601 cattataaaa cagtacaatt ggcggatagg tcaagtgaag cagctgaggg ggatatttgg
    21661 gatcaaagca agtgataaac gactgtttcg attggatttt gccccagtta gcgtgaattt
    21721 cctgccaact tttgcgaaac agctcccggt gtttggttat tttacctatc ttaatgatgt
    21781 cttcaaatcc gatttcttta taaggatact gcatcatcca gttagcaagt gcccattttc
    21841 cttgactact gattcctttt gtataagcaa gtttcaataa attttcttct atttgataca
    21901 tcataaaacc gcctttcgtc tctctttcta ataagatacg taaaagcggc tgttttttat
    21961 tgtaaatatt tttttacagg tgcaaaactt ttacgatgga ttggcgtaat tccttgtcgt
    22021 tcaagtcctt ctaagtgttc ctttgttcca taacctgcat tatgcgagaa accatatcca
    22081 ggataaagca cgtcatattc tttcatcaat tggtctcgat atactttagc aatgatactt
    22141 gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta ttttttcctg ctgaataggc
    22201 aaatccaagg tcatcgcatc gatcaacaaa tagcttggta caatggacaa tttttctact
    22261 gcttggatca tcgctaattt acttgcttca taaatattca catcatcgat tgtctgctga
    22321 tcaacgatcc cgatcccgat tgccagggct ttttcttgaa cttgttgata aagaagttct
    22381 cgctttttct ctgataattg tttggagtca ttcagcccta atatgatctc atttttcggc
    22441 aagataactg cagctgccac gacaggtcca gctaatggtc ctcttcctac ttcatcaatg
    22501 ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt caaagcaaaa catttcttca
    22561 tatttttcct taattaatgc ttcctttttc aatcgtcgtt caaaagaagc cagtgcttgc
    22621 tgcacgcctt tgcgtggatc atttcttaat gattctagtg taggatcatc tattctctgt
    22681 atgcttttca atcgttcttt gatactttgg atggattcag tcatcacttt tttgctccat
    22741 ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct tgacgaatat catgcacgac
    22801 taattcgctg ccacgatcat agtcttcttt atacccacgt tttgctgtaa tatccatcag
    22861 taattccggt gccaattttt cttcttgctc gattgataaa ccataacgat cgctgattct
    22921 tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc gctaaatcat ctaaatggac
    22981 aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg ccgatttctt catcttcaaa
    23041 tttgggccat aaaatccctg gtgtgtctaa caattccaac tcattaccta aacgaagcca
    23101 ctgctgacct tttgttacgc caggcttatt tcctgtctgt gcaattttct tgcctactag
    23161 tcgattcatc aacgtagatt tcccaacatt tggaatacct aagaccattg cccgaatagc
    23221 gcgaggcttg atcccccgag cagcatctct tgcccgtttt tctttcaatg cttctttagc
    23281 cgcaggtact agacctttaa ttcctttact ttcttgtgcc gtgatagcta atgcaagatg
    23341 gccttgttta cgaaaatatt cttgccacag ctttgtctgt tgtggatctg ccagatccgc
    23401 tttattcaat aaaacaaggc gaggtttttg ctgaatgatc tgatctagca ttggattacg
    23461 agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg atgtccacat atttcatttt
    23521 ttcagaaact tctcgtcggg cttttgccat atgtcccgga aaccattgga tggtcattgt
    23581 tctctccatt cctttcatac cggaatattc cccggcttta cttgtttttt tctttttcaa
    23641 tataaattca cttttttatt ataaagaaat tccatctatt agttaagtga aagtgaacca
    23701 gtcctcattc tatcacatct tcactttttt gatgatgcaa atttttattt tggtttctat
    23761 atatgaatct aaatttttga cagcgtttcc taaataagag atgatagacg aacaaaacag
    23821 aaaggagacg aaacaaatga atagtatggt ttttgttaat tttcctgttg ctgatgtcaa
    23881 tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa aatgaagaat tttctactga
    23941 agaagctggt tcaatggtat gggatgacac tttttggata atgcttctct ctcatgattt
    24001 ttataaaaaa tttataaaag ataaagaaat agctgataat aaaaaaacaa gtggtgcttt
    24061 gatttcgttt agtgttgaaa atgcggatga agtaaaaaaa tttgctcaaa ttgcaaaaga
    24121 aaatggtggt actttccacg aagtagatat gggaatgcct gaagaagaaa tgttttcatt
    24181 agaagtccaa gatctagacg gaaatacatt agaacccgta tggatgaaga tgtaagcaga
    24241 taactaatct gacgtttttt ataacaaaaa aacaaaggcg gatgatacga taagtattct
    24301 ccgcctctat tttttttatt cgtacaatct cttttttaca gtttataaaa gcagtcttct
    24361 cacccttttt ttaattgtta gatgccagtg tatgtaaatc accaatgtgt catgacacat
    24421 tggtgatttt atggtatcct tttattatta actgacaaag gagagaatat tttgaacact
    24481 ttgattgcaa aagacgtttt tagattgccc gaaatgaatg caagattgtt gtcgacctct
    24541 gctcttttat tagcccttga tgtcatctta tacaagatag cgattggtcc tgcttatttt
    24601 caaatcacat tcggattttt aagtatggcg cttatgggtt atctatatgg accgatctgg
    24661 gctggtttgc ttgaaatgtt gtcgaacact ttaaatttca cgatatttgg ttcaggaacg
    24721 tttcaagctg cttttcttat cacggctttc cttggtggat ttattaatgg cttattttta
    24781 tatcataaaa agataaacct gccttccgtc atcgctgccc aattattgat tatgatccca
    24841 gtcagccttt ttatcaattc ttggctgatg tctgttttgt ttggtgcaga tttcaaagta
    24901 atttttctag gtagattcat caggaatatg atccaaatcc ctattcaaat aatggttgtt
    24961 tattcattta tcaaaacttt agaggctaga aaagtgttta aacgagctgt ttgattttac
    25021 gtactaaggt agtaaactga ttttggatta aacaataaag cgtgcagtct caacagactg
    25081 cacgctttat tgtttctcac tggatccatt ttatttcatc aagtggataa tagacaaatc
    25141 ttgctttacc tagaatatca gttttattga tcgtgccaaa cgaacgacta tcttttgaaa
    25201 ctcttcgatt atctccaagt acaaaataat tatcttccgg aagcttggat tgtcccgtta
    25261 gctcttgtag tgtaaagtcc gtcgtatagg atgcatttgc atgatctgat ttcaggtttt
    25321 tagttagata aggttcctta actactttcc cattgatctt caattgatca ttttggtagc
    25381 tgacattttc tcctggaaga ccgattactc gtttgatata aatcgtgcca tctgctaatt
    25441 gaaaaacaac aatatcaaat cgtctgatct ccgaaaattt ttccatcaca accatatcac
    25501 cttgcttcaa cacgttttcc attgaattgc cttccactgg taccgggatg aataaaaatc
    25561 cccgcaacat gaacgcaagg aatacagaaa caagtaagta tttgaataca agccagaagc
    25621 gattaatata gcgttgtttt tttgtcaatc tctatcaatc catccctctt tttttcattc
    25681 tttctttatt ataagaaaaa tatcaaaaaa atgcgactag atagctgaaa taaaaataat
    25741 tctttcaatt aggctattgg acaaaaaact tttttgatca taaagtacaa aactatctct
    25801 tatatgtcat gattattttc taaatatttt tcccatgctt cgtcaaagat atccatggac
    25861 ggcaaatagt cgacattcag ttccagataa ttagaaattt cttcgtagct agagctgtgc
    25921 ttaggaaatt ggatgtcttt accggcttct gtagcgaaaa cagataaagc atcatgtgtg
    25981 ccaccgcctc gcagtgtcat taaataatga aaaaagcttc gattcaccct tcgttcctcc
    26041 aattttcttc gataaaggca gcccggcgtg catggtttct tttaaaattt tcaggatgct
    26101 ttttataata ctcttgatgg tcttcttctg ctagataaaa tggttgggct ggttcgattt
    26161 ttgtaacgat tggctgttcg aaacgtccac tttcagctaa ctgtttctta cttttttcag
    26221 caatgatccg ctgttcttct gtcaaataaa agatcaccgg gcgataattg tcaccgcgat
    26281 cctcgaattg tccgaaagcg tcagttggat cagtttgctg ccagtaaagt tctactaatt
    26341 gttcatatga aatcaatgtt ggatcataga taatctccac tgcttctgta tgtccagtcg
    26401 tttggctttt tacttgttca taagtcggat ctggtacatg ccctccagta tatcctgaca
    26461 tcactgtatg gataccagga agcgtgtcga aaggctggat catacaccag aagcagcctc
    26521 ctgcaaatat tgctcgttcc attatgattc cccctctaat actactcatc cgatgatttt
    26581 ttattttctt ttgtcttgct ttcagtactg ctggtgctag ttttgttggt atttgagttg
    26641 tttgaagcat cagaggtatc ttttggcaaa tatagactaa accgaatgtc atcatttact
    26701 aagtcgattt tatctgcttt aatatacatc ccgttttgca ttcggaattg atccaaccta
    26761 agcatgatag tcagatcatc tgtattgact tcgatccaat ctggtaattt atagtttctc
    26821 ttgatcatat tcatcacatc tttgattggc aaactcaatg ttccgataga aagacttcta
    26881 gcttttagtt ggacatttcc gttttccatc acatacggat caaaatataa atagaaatcg
    26941 ataggaaaac ctagtacttc gaaagtacca ttgagtagtg cttcattttc caaataaaat
    27001 ttatattcaa tatcactatc tttttgatac tcactaagga aaaagctgat gatttgattg
    27061 actttttctt tatttgaatt gatcgttaag acaggatctc cttgtctttc cgtaagagct
    27121 ggtatttctg ggagttctgg ttcccgatta gcaaaaatac ggctgcctaa aaatacggtc
    27181 gaaccaagta ctaccccaag aaggataagg aaggcgaatt tccagtaatt tgttttcttt
    27241 gttttgtttg aagatacgtc agatcttgtt ttcatgttta ttgatcatcc tccttctcta
    27301 accattggtt ttgtgtctcg atcagctgat ctctcactgc atttgccatc aattgataac
    27361 ctaaattatt tggatggaaa tgatcttctt catagagcac attatttttg atgccattta
    27421 gattttctgt cgtgctagtt gtttgatctg ttccttcttc tgtgacacct acttcctgat
    27481 tcaatccttg ataaagcaaa tcattgattg ggataaagta gcagttcggc attttttttg
    27541 tcaccgtttc tgttcctttg ttccaattgt taacaattgt ctgcatgtca gtgatctcag
    27601 gaaagttcaa ataaaatgga ttgtagatgc ctaaaatgta aataggtgcg gttttattga
    27661 gggatcgaaa ttcttccaat agttctgtga cattttcctg atagtttttc agtggctttt
    27721 taaaggaatt gaccgttaac ccgaataaat tattttggat aactttcatc aaatcattac
    27781 cgccaacagt cagtgtgata aaatcagctg ttttgatatt gtttcgaatt tcttcttttg
    27841 tttttactcg ctttaagatc tggtcacttc gttcaccatt tacaccatag ttttctgttt
    27901 caaccgcggt caaattataa cggtctttta gatcatttgc gacaagggga acgaatcctc
    27961 cttgcttggt ttgatctcct actccttcag tcagcgaatc tccgatagct atgtagtgaa
    28021 gtttttcttt tttatttgga tcttctctca cagtagttac tggtttcatt agtgggccag
    28081 ctttcggtat agaagcagct aaacctgcat agatgatgat cgcaattaat aatgggaaca
    28141 aaacgaggat caaatgtcgt ttcgttaatt tcattttttc acctcttgta ttcgattggt
    28201 tctaatgtta tcaaaaattt tatcaaaaag gtagtcttta tgaaaaaaga aaacaattat
    28261 tttcctaatt ttataaagaa aaagagaata agctgcaagc ctttcaggct gagggcttat
    28321 cctcttttcg actaatctac ataatacatg attgcaaacg cgtctttacc agtatgcgta
    28381 gcgataaccg gattcgtatg cagtacaggg atatgcatgt ccgggaataa ttcttgcaaa
    28441 ccctctttga attcttgcgc caattctttt gcatccgcat gtgaaatacc gatttgtcgc
    28501 acatttggca attctttcag ttcatttttc aaattattaa accatttatc gaacgttttc
    28561 tttcctcgcc ccttagttac agggattaat tcagaatggg cgaaattcat caccacgcgt
    28621 atgttcaaaa cactagacag caaaccagtt gttcggctga ttcttccacc ttttaccaag
    28681 ttatctagtg tggatattcc gatgtataat tttgtattgt cttgtacttt tttgacttct
    28741 tcaaggattt tctctactgt tttaccttct tttgctaatt cagctgctcg aattacctga
    28801 aaagataatc cttggtcagt ggtatcactg tcgataaccg ttactttcgt ttttgttaga
    28861 ttactggctt gtcttgctgc ttctactgtt ccactcaagc cttttgtcat atggatcgaa
    28921 atcacttcac tgccgtcttc tcctagctca tcatacatcg agatgaattc tccaattggt
    28981 ggttggcttg ttttgggcag attttttgct ttcgccatca tttccataaa tctttcgccg
    29041 tctagttggt catcatcggg atatactgtt ccgtcgatca tgatggatag cggcatgaca
    29101 cgaatgccta aagcatcgcg tacatttttt tccatcgtac atgaagaatc tgttacgatt
    29161 tttacatttg tcattatttt ctctctctct ttctttaaca agatgcctta ttcatgttaa
    29221 actacatgta agacaatttc tatttttagt ataacagaca aataatgatt tttcattaat
    29281 tacacctagt aaaggagccg ttttcatgga aaaaacgaaa tttaatcgaa cgtatgaaat
    29341 attaaatgaa gtgtttaatg cagtcaccca tggaattggg actggattga gtatcgcggg
    29401 actagtggtt cttttagtaa aaggtgccca cttgcattcc tccctccata ttgtctctta
    29461 tgctatctat ggatcgatga tgattctttt atttttaacg tctactttgt ttcattcatt
    29521 gatttttaca aaagcaaaaa aggtgttcca agtattcgat catagttcga tttttctttt
    29581 gattgcagga agttatactc ctttttgttt gctgagtatc cgaggatggc taggttggac
    29641 tttatttgta ttaatctggt tgtttgcgat tcttggcatt atatataaag cgttgacgtt
    29701 gcataaacgg gagagagtat caaaagtatc tacgatcatc tatattttta tggggtggct
    29761 ctgtgtcgtg gctgctgttc ctctttatca ctctcttgga atgactggtg ttattttgat
    29821 ggtagcaggc gggctttctt acacgatagg cgcattcttt tatagcttga attctatccg
    29881 ctatatgcat gttgtttggc atttgtttgt tatgttaggt gcaggtttta tgtacttctc
    29941 tgttttattc tttacttaat aaaaaaaata aagctatgga tgggacagct ctaaataaca
    30001 gctagtctat ccatagcttt tttatatcgt caattcgcct gtcccgcttt ttacaggatt
    30061 ctcattacgc tgatacgttt caaattgatg cggataacga tttttttcat ccatttctcc
    30121 ttgactagta ttgatcagtg accaatcaga ccagtcaaca ggtggaaaat aagcatctcc
    30181 atcgaataca tgatggatga ctgtacggta taatatcgta caaaatggca gaaattcttg
    30241 ataaacagct gaccctccag cgatgaaaaa gataccgtct gtttcttttt ccagagcaag
    30301 cacgtcttcc acactatgca tgacatgcac gccttctgct ttataggaag aatctctcgt
    30361 caaaacaatc gtctgacgat tcggcaacgg acggctaccc atcccttcga atgtttttcg
    30421 tcccatcact agtgtatttg cttctgtcat ttgtttgaag aatttcagat catttggcaa
    30481 acgccaagga agacggtctt cttttccaat caatccgttt tcatcctgtg cccaaattgc
    30541 agctaacatt ttctcatcct ttctgattct aaacagcgat aggagctttg atcgccggat
    30601 gtgggtcata tccttctaat tggatatctt ccatctcaaa atcaaatact gactgctttt
    30661 cctgattcaa aactaaagtc ggaaatgcgc gtatttctcg gctcaattgt tctttcattt
    30721 gttcgatatg attgatataa agatgagcat ctcctaatgt gtgaacaaat tcgccaaccg
    30781 ctaaacctgt ttcatgtgca attaggtgag tcagcaacgc ataacttgca atgttgaacg
    30841 gaacgcctag aaacacatcc ccactgcgtt gatacaattg gcagcttagc cgtccctctt
    30901 gaacataaaa ttgaaacatc gtatgacaag gaggcaaagc catgctagga acatcttccg
    30961 gattccatgc agaaacgatc aatcttctag aatcaggagt ctttttgatt gcttcgatca
    31021 cttgtttgat ttgatcgata aagccaccgt cttttgtttc ccagtgacgc cattgatagc
    31081 cgtagatgtg tccaagatcg ccgtatgttt cagcaaagga atcatcggtt aggatcttct
    31141 cgcaaaaaag cttgctctgc tttttatatt cttcattgaa ttcaggatct gtcaaacatc
    31201 tatggccaaa atcagtcata tctggtccat tatagtctgg ccttttgatg tagcgttcaa
    31261 atgcccattc atcccaaata tggtttttat tttgtaatag atagcggata ttcgtatctc
    31321 cttttaaaaa ccaaagcaat tcgcttttga tcaatccaaa aggtactcgt ttcgttgtta
    31381 acaaaggaaa ccctttttgc agatcaaacc gcatttggta tccgaataag cttaatgtgc
    31441 ctgtgcctgt tctgtcttct ttacgatgcc cttcttccaa caattttctt cctaagtcaa
    31501 gatacgcttg ttccatcctt tttcctccta atcttctacc cattcactca agtattccca
    31561 ccgtgccatt ttttcttcaa gttgtgcttc tgtttcagat acgtcgtttt gcaattcttg
    31621 taatcgagtg aaattatctc cttgatgatt catctcttct tggagcaatg agattttttc
    31681 ttcaagttct gcaatctcat cttcaatcgt ttcccattct ttttgttcca tgtaagataa
    31741 tttcttcttt tcagcaggtt ccttgctctg tatttttggt ttcgcttttt cagcaggctg
    31801 ctgggtctct ttttgcttca gtaaataact ggacatgttt ccgtaataaa gttcttgttt
    31861 tccgtttcct tggaatacta ataacttatc tgctaccttg tccaagaaat aacggtcatg
    31921 ggaaacagtg atcacagcac ctggaaaact tctaaaataa tcttccaaaa tcgtcaatgt
    31981 tgctatgtcc aaatcattgg tcggttcatc caataataaa acattaggtt gctgaatcag
    32041 cagtttcaac aaaaataacc ggcgtttttc tccgcctgaa agtttgccta tcagtgtccc
    32101 atgcataaat cttggaaata aaaatcgttc aagtaattct gctactccga tttgactgcc
    32161 atctgctgtt tttgcttctt cagcagcttc ttgtaagtaa gcaatcatcc gtttatctgg
    32221 tgccatttct tcattttctt gtgtataata cgcaagatga accgtttcac cgatagacat
    32281 tgtaccgcta tccaggggaa ttctgccagc tagaatatta agcaatgtcg attttcccgc
    32341 accattttta ccagtaatac ctagtctttc ccgagattga atcaataaat ctaaatgttc
    32401 tagcagtgtt tggtcattta tacggtaact gccatctttg atttccagta cttttttccc
    32461 taaacgttgt gtcgctaggt tcagctctaa atcatcttct tggtttactt ggtacaagtt
    32521 ctcctttaga tcttcgaaac ggttgatccg tgcttgctgc ttggtgccac gtgcctgtac
    32581 gccagccctc atccaagcta attcctgttt ataaagccgc ttgcgctttt cttcttgttc
    32641 tacttctaca cgatcacgtt ctgctttttc taagacgtaa gcttcataat ttcctttata
    32701 ctcgtataat tttccaaatg aaagttcgaa gattcggttt gctactctat ctagaaaata
    32761 acgatcgtgg gtaaccatca atagtgcgcc acggtattgt ttcaaatagt tttccaacca
    32821 ctcgatcgct tcataatcta aatggtttgt tggttcgtct agtaatagta aatcgggttc
    32881 atcaatcaac acttgcgcca gactaactcg ttttttctga ccacctgata aagtactgat
    32941 ttttttatct aattcactga ttcctaattt ttgcaaaata atctttgcgt tggtatctgt
    33001 agtccaagca tcttctttat tcatttgttc ttctgcttta gcataacgtt tttgcacatc
    33061 agggtcttct ccgttttccc ctagttcgat caatgcttcc tcatatgctc gaacagtttg
    33121 gatcaagggg ctgtttcctt gaaagactgc ttggagaaca gttaattcat cggaaaattc
    33181 agctgcttga gaaagataac caatacggta atcagttgga taaaacactg tttgccgatc
    33241 accgtcgctg ctatcgatac cggcaataat atttaagaga cttgttttac ccgtcccgtt
    33301 cgtcccaatc aacccgatac ggtctttttc gtggaccaaa aaagaaatct ggtcaaacaa
    33361 atctttttca ccataagttt ttttgaggtc agttactttt aattctttca acatgttccc
    33421 tccttatctt ggatattgta tcataaaaat agtgaacttc aggaaaattt ttttggtatt
    33481 tgttcttttt gtgttctcaa aaaaagaaca ggaccgattc gatcctgttc tgatatgttt
    33541 tgttttttat aaaacttgaa aatttttacc tttttatttt attggactcg ttttttttga
    33601 ctttgctcga aacgcaaatc ggaaagaaaa gttatcagat ggtttgttca tcgtataaga
    33661 ttctaacaaa ttgatattcc tgcgatagct gttcgcagaa aacgtcgtga ttttttcagc
    33721 aacttcgtat tcatcaatga tttttctttc aagataataa cctcttagca tttttttcgt
    33781 aaacagacta tcttgctgga tcatccacga agaaatcaaa ttccccgagt tcatttttat
    33841 tccttcaagt ttgcgttgct ggataaaaaa ttgaaccatc gtttcatcat aaatatcagt
    33901 caagttatct aaactttcaa taatcaactg agtatttttt tgatatgtct ctctgatttc
    33961 ttggatatct tcatcactaa taaatgtctg acgatcagca tatcttcttt gccaaaacat
    34021 tttcggatgg agaacgattc gcattaacct acggacaaca atgaaccaaa agccgataaa
    34081 agacaaaatc tggcttgtga tagaatgttc gaatcgagaa agaaaacggg agtaaaaacg
    34141 ataaccatta gaagaaagct tgcccgaacg ataactttca tccaatccat cccgttcgat
    34201 cgacaaaatc agtgcttgta tttcttgtac ttcttgcttt tcatcatcgg ttaaatcttc
    34261 caagtacaga tcacgtatcc gttcttgata attttctatc actgcttcag tggctaaaaa
    34321 ttcttttgta ttacgatcaa tttcctttat ttcttttcgc aaaacttcaa cgacctcttc
    34381 taaaatcatc aaagcattca aatccgtgct tttcgcttcc tctgtttccg tcagcatagg
    34441 caagacaatg atccctatga ccaaagtcac taagatgaca cccgccgtca gaaacaaaag
    34501 caaagaccgc tggtaaaaca tcatattatt gattgaaaat ggcaaaataa aaatcgtagc
    34561 caaactgact gttcccttta caccgccaaa tgttaagatc aagatctcgt tcaactgttt
    34621 tttgaacttt ttactgccat ctttgaatac ataaaaaaga ctgatagaaa tgaatctgat
    34681 cacaaaaagc atgacagaga ttaatacgat aatcgctaat aaaagaccgt ttggatataa
    34741 accgtctccc catactggcg agaacacttg cgtcaattcg attcctaaaa atataaatac
    34801 taatgcattc agtgtaaaag caatagttgt ccatgtactg tgggacaagc tcgacaattc
    34861 tgcatcaaac acagagattt tcctaaaacc agaagcttgg agaatacctg ctgccactgc
    34921 agcaatgatt ccagatacat caaatgcctc tgccaataca taggctagaa aaggaagcaa
    34981 aagttcgatc agcagatacc ctgtgacatc ttgtgctgat gctttttcga tcaaatgaat
    35041 tattttttgt ttgaaccaga caagcaaaaa gccgatcaaa gctcccccaa tactagaaac
    35101 tactagactc atccccgcgt ttactgctga aaaagatccg gtgatcagcg caccaagagc
    35161 aaactggaag gcagtaacac cagatgcatc attcaaaaga ccttctcctt ccaaaatatg
    35221 catcgccttt ttagggatat tcactcgtcc agaaagagaa ctgactgcta ccgcatctgt
    35281 cggacctaac gctgctccaa aagcaaagca ggcagcaagt gggacacttg gcaacaggaa
    35341 tgataacgtc gcacctaccc cgacaagggt caaaatgact cctccaaatg ccaagaacaa
    35401 aattgtcccg aaatttttga gaatcgaaga aatatctgct ttttctcctt ctctaaataa
    35461 caatggtgca ataatcataa ctaagaacaa ttcaggttca aaatcgattg attgtcccca
    35521 ctctgtcaga cctaatacca ctccaaggaa aatttggatc agtggagctg gaatcattgg
    35581 caaaatgcgg ctagctatgt tcgataaggt gatcgttact gcaaatacaa taactaactc
    35641 agcaaattcc atcgtccatc ccctttattt tttattttgt gtaagcattt ttaaaaattg
    35701 ttcttttttc tttaaaattt tggtttcgac tacttcaacc tgtgtttggt tcccctctaa
    35761 ttcacactgt acacgttttg actccaaacg aagcagatca tgtttcagac gttccaactg
    35821 acgatacatc aatatatttc ttttacgcaa cttacaaagt atttgacggc ggtcatccat
    35881 cgaaagggcg ccatagcgat catgcaacag ttttttttgt tgttcaaaac tctctctcat
    35941 tttccagatc tcctatacac aaacttccat cattatagca aagttctttt tggatgaaaa
    36001 atatttcatt gtttcatcgt ttattgctag ttagaaattt ccataaattt ttcaacgcct
    36061 cattctattc caaatagtat cagatatcaa gtaaaactta agaaagataa aagtttctga
    36121 caaaaggatg tttactttcc cattcctgcc tttcttgttc ttttatcaca acaaaaaaat
    36181 ggggctgcga caaaaatctt gtcacagtcc cttattctga ataaacggtg tggaagagac
    36241 agcctatcag tagtaataag acacatctct ttcacagcct catttttgcg taaaaatcga
    36301 tttattcgaa aaattccccg tatacatctt ctacacgttg atctggattg atcacgatat
    36361 acaagttacc tgttttttcg ctgatttttg ttgattgata ttgattgtct aatccttctg
    36421 ttcctttatc tagatatggg aagtacacaa gatcaggtgt gccatttcgg taaaggattt
    36481 ccattcgctc gatatcttcg tatttcaatg cgcgattaaa catgcccatt tccagtccgc
    36541 ctaggttgat atcatgtgtc gcaactgcgt ccccttcatg gaagatttcg attttaaatc
    36601 cagcacacgg atggatttca acaaattcac tgccattgat tcgtccaaaa cttgttgtga
    36661 tttgtttgat ccaaagatcg ccgatatgac ggcggttgat cgtccaggtt tctccatttc
    36721 taaaataaaa ttttagcgct gtcatttctt tactcattaa aaagactcct ttatgatgta
    36781 atttattctt gtactacctc agtttagcac aaatttatgt aagtggtcac atcttatgtt
    36841 ttttcttcta atgacgtatc tgcccacttt aagtagccaa caaagctgag cagatacagt
    36901 ctagtgtttt ttaaatcaat ttttctttcg ataaaaaatc caaaacctgt tttttatcat
    36961 tttttattct ttgttctacg acggcttttt cagctaattg caaagcttca ctaacccact
    37021 taccaggttt ttttccagtt tcttctagca agattttacc atctactgct aattcgctta
    37081 atgacttgat aggtaaacga tcataggatt ctcctacggc atccatctga ggtgtttcgt
    37141 caaaatagat catcaattct tctacttgca gtgcatgttt tttgcctaag aagtacaatt
    37201 cgaatggttc caaagctcca gtttctcgtt gtcgtagtcc ttggacaagt gcttgaacgt
    37261 ctttaatcat ttggttagaa cacttccacg atttcaaaaa ggaacgaatc tcattttctt
    37321 ttagatccag ttggctgatc aataatgccc atgcttggct ctcttctttc aatgggacgg
    37381 atggtaattc agcaaaacgt aaaagtgctt cgccttcttc tttcaaaccg ggacagcaaa
    37441 tgtagcactc tgtttcaata aatgcatgga ttcccgcggc ccgttcattt cccaagagca
    37501 tcttgatgaa ttccacattt atacgttcta cagatatttt acctaatagc tcgtggtttt
    37561 ctttgatggc gtagaaggtt tcttcctcta atttgaaccc tagttgactg acaaaacgca
    37621 acccgcgcat cattcgtaag gcatcttcat ggaaccgctc atgaggattg cctacagctc
    37681 gcaagatctg ttcttctaga tcttgcagac catcgaacaa atcgatgatt tctccatctt
    37741 ctttcaaagc aaacgcattg atcgtaaaat ctcttctttt caaatcttct gccaacgaac
    37801 gaacgaattc gacatgatct ggtctacggt agtcctgata agttgattcc gtccgaaatg
    37861 tggtgatctc atattgttca tcattttcta ataccaacac agttccatgt tcgatcccaa
    37921 tatctatcgt ttttggaaaa agctgtttga tttcttctgg aaatgcgctt gttgcgatat
    37981 ccacatcatg gattggctgg ttcagcaaga tatcacgaac acttccacct acaaaataag
    38041 cttcatatcc tgcattctct aattttttca gaactggaat tgccttttgg tattccagag
    38101 gtaattgttt taatttcatt tttccaacct ttcctgcgtg tttcttgtcc tgagataaag
    38161 actcagttgc atggtgactg tttgacaagt ccgtttcaga gtcttcaagg ccaggaccac
    38221 ttcatcttgt ttactcgtcc ttacgctcaa agcgattatg gtctctttga ttgaattttt
    38281 ccatgttttt ttcaaaaact tcagttaagt cgatattcaa tgaattagcc atgatcatcg
    38341 taacgaataa aacatcacca agttcttctg ctaccgagtt aactggttca gaagcctttt
    38401 tgcttttttc tccataagtg tggttgactt ctcttgccaa ttcacctact tcttcagtta
    38461 atcgagccat ctgagctaat ggagagaaat accccacctt gaattgctgg atatattcat
    38521 ccacttcctg ctgcatactg tataaagaac gctttttatc catctttttc cctccaaaac
    38581 tatcttacca aaactttaac gcaaatccta gataagagca attgagaaaa tcgaatttcc
    38641 atgctacatt taggaaggaa tttctaaatg gaaattctgt gaaaatacat aattgaaagc
    38701 tggaattcat atggtcaaac gaaaaacagt aaaagaagtt ctttttatca ttataggaac
    38761 aagtatttat gcatttggtc ttgtttactt gaacatcgct aatcatttag cagaaggggg
    38821 agtctcaggg atcaccttga tcttaagagc tttatttggt attgatccag cttatacgac
    38881 tttattaatc aacatcccat tgattttact aggaggaaag atcctcggta aacgttcctt
    38941 ggcctatact gttctaggta cactttcttt gtcattcttt ttatggatct ggcaaagggt
    39001 tccactagaa atcaatttac agcatgatct actgatcgtt tctctacttg cgggtttagt
    39061 tgcaggtgta ggaagcggga tcgtctaccg catgggtgga acaacaggcg gcagtgatat
    39121 tatcgctcgg attttagaac aaaattacgg catcactatg gggcgttccc tattggcatt
    39181 tgatatcatc gtcttacttg cttcattaac ctatatcgac ttgaaacgta tgatgtatac
    39241 gttgattgtt tcgtatgttt tcagtcgtgt gattgattcg atcttagatg gcggatattc
    39301 tgcaaaaggt ctacttgtcg tctccaacaa aagcgaagag attgcaccac tactgatgac
    39361 cggtctgcaa agaggtgtga ctttcctttc tggagaaggc gcattctctg gtaattcaaa
    39421 aaaaatcatc tatatggtcg ttagctctag tgaattaact gaagctaaga ggatcgtcca
    39481 cgagattgac gaaaaagctt tcctttcaat catcaatgtg catgaagtgg aagggcaagg
    39541 ctttacttat ttgaagccac agacaagatt actgaaaaaa ctataagaaa aaagacaaag
    39601 ataggctatg aggaaagatt ccacatccag ctgacctttg tcttttttag tactatacta
    39661 tttttaacta attgttatgt tcacttaccc ggttactttt cttttgtgtc tgcaaaagga
    39721 tctttcggtt aacttcatca gataatttca aaaaagcatc cctatctcca tcttctagtg
    39781 cttgatcgat ttgagcataa agcattttta gttttgcctc ttcttctttt tcttgaaaat
    39841 atcgttcaat taattcagta tcttcttcac tgactgaaga atcccaagaa gaaaatggat
    39901 tgtcttctaa gatagataga tattctcttg tctgccaagc attttcgaat atacattcca
    39961 catataaagg ctttttccaa ttcaaacgga tttcatgaaa aatctggtct gtgtccgtaa
    40021 attcttttcc ctgcaaaaac aatttcatgg gttccccttc aacagataga tcactgatct
    40081 gcagtcctcg atccgcaaaa ttcgcccctt caacaaagtg gacattgctt aaaatcgctt
    40141 catgattggc aagataattc aagatccaaa aaacttctct tcggctaaaa gatacatgat
    40201 tgaccatcca cgttaaaaaa ttcttctttt cccgtacatc aattagcatt tttctcacct
    40261 ctctgttagg tcatctaata atgacagtgc ctccatatct cctggttcaa gtgttaaata
    40321 ataagtcaat aattcttgcg ctcttttcag ctgcccttct tctcttaaga aaaaggcata
    40381 ctctttcaag aaatctggtt catgtgatag ttcatgataa gcctgttcat agtgtacagc
    40441 tgcaacggta aagtcttcta attcattata agcatgggct aggttccatt ctgcataagg
    40501 attagcagtt tcttccatct gattgatcac ttgtatcaca tcttcatatc tttcatcatc
    40561 caagtaaaga ttacttaaag taagtaatgt ttcatcttgt ttgtctccta cttctaaagc
    40621 tttcagtaaa agttcttctg ctcgctgtct atcatgcaga cggaaagcat tttccgaggc
    40681 caggtgataa agttcgactt ggtaaggatt ttctttgatt ccttcttcta acactgtttg
    40741 tgcttcttca atcatttctt cttcttgaag agcttgtcct agatatagat ataacgattg
    40801 ataatgcgga ttaacttctc gaagttcttc cagatagttg attgcctgct gattttcttt
    40861 caattgaaga ttcgtaaaag ctagctggaa cagtcgatca tctgtccttt cttctttcag
    40921 tgcttcttct aaaggtacga tagctgcttc gaattctcct tgcatactta aggattgtcc
    40981 taaacgttcc tgcatcgaga ttcctgaaat ttcatttatc ccgcttgcca acaatgtttc
    41041 ataaaccact gctgcctctt tgaaacgatc aactgaaaaa tacaattcag ctagcgcaaa
    41101 ctggatcaat ggttcatcag gaagcaaccc agctgcttct ttcaattttg cttcactcac
    41161 ttcagggatt ccgataactt ggtacaaatc agctatcgcc aataagcttt gcggatagta
    41221 atcactttgc ttatccactt tttccaggta ttcaaaagct aagtcgatct cattatcttc
    41281 gattgcaatt tctgctaacg ggatgttcag tccgtcattt tctgggtatt ggttgatcag
    41341 ttgttcaaaa atctgttttg cttcgatcaa aaagccaata gccagcaatt cttcgcctag
    41401 ttcagctaat atatctgctg ggtcttcttt taatgctttt tctaaaaaga tttgtgcttg
    41461 tgacaagtct tcatctgata aagcttgcaa cattttttcg ctatttgtat tcatttcaat
    41521 tccctcttat ctcactgacg atttgttgaa ataccgtcat tgttctcaat aattcttttt
    41581 cgtttggatg ttggtatccg tctattatac ctattttttc taaaacaagt atttttttgc
    41641 ttttttctgc caaaaaaaga actttttcca aatactccat ctttgaaatc tgttccagcc
    41701 agtggattgg atagccaagc aagtccagcc acacaaaaaa gttttttagt tggaaatcaa
    41761 aaggatatgc ttggagattc cacacgaaat gaaacatcat tcctaaaaat tttttcatac
    41821 tagcagaaag tagatggcca ttttcggtca gataaaaagc ttgttcaaac agtttaccat
    41881 aatttgacag gttttctgcc tctaattgat aaaaatagat catttcttcg atcatcccgg
    41941 caaacggccg agtcgttaat tgctttttat ttggatagtt tttatacaga ttttgcaaaa
    42001 aacgatgatc acaaatcatt ccgcaacaga taaaagtcaa aaagtcaatc tgctttccgt
    42061 ccacctgccg ctcgctgatt gtttgatcat aaacgatgcg ttctggcaga ttgatggttt
    42121 gcagcactac acgatttggt agctgcaaaa tcgtccgctg cggtgttaat gctcggctta
    42181 gagaacggat cgatacaggg atcatccata gtgatgaatc aagggttgta tgtttttgaa
    42241 aaaaacctgc cagatccatg atcccttcat ttccaaatcc gatgataaac atggattgat
    42301 tttcgggata gcgtttgacg aaatcaagca ggttctttaa ttctgttaaa tgattgcatt
    42361 gagtgttggc acaaatatac cagtcattgt ttgcttgatt tgcaaataac gggttgatct
    42421 tatctgcaaa caaatcataa tagcgctgat tcgttaaaaa taagattggc tgatcaatgg
    42481 aaagttctct tagctgactg actagcgttt ctccgtaaaa cactttcgtc aacgcatctt
    42541 ctacttgata atcaagttcc atacgcatcc ctcctcttct tatttttatt ttaccattag
    42601 taagcaaatt ctagaaaaga taagagcaaa gctcacctca taacttgcaa ataaataaaa
    42661 attaaataaa atacatgtgt tttctatgta tgacatacgg attcggaatc tctgtctctt
    42721 atttattcct tttctgctag gaaataaaaa gaaaagcact tgtagcccta gagccacaag
    42781 cgctttttca actgtcttat ttaacagcgt cttttaatgc tttacctggt ttgaatgcag
    42841 gtactttgct agctggaatt tcgatttctt caccagtttg tgggttacga cctttgcgag
    42901 ctgcgcgttc gcgaacttca aagttaccaa aaccgattaa ttgaactttt tcgccgttag
    42961 ctaaagcatc ttgaatagtt gaaaatacag catctactgc tgcagttgca tcttttttag
    43021 ttaaatcagt agctgaagca actttttcga ttaattctgc tttgtttgcc atggatagat
    43081 tcacctcctc cacaagagat atgatagaaa tatcatttag ttaaaacttt ctgagtggtc
    43141 cagaagttta aaaagaaata ttgattaact tcaatattct gttatcacga tatcatatga
    43201 accgcttaat agcaaggttt ttaccaattt tcacatgctt tttttagtca tagaaacgta
    43261 acaaacgaaa ctgtcttttt cttttgaaaa aaaaacgtcg atatggtaaa atgcctattt
    43321 tctcctacga gggataattc ggatggacgt cccttcaaaa gtaaatgctt ttctgatttg
    43381 attttctaag aaacgttcat aagaaaaatg catcaattcc tcttcattga caaaaacgac
    43441 aaatgtaggt ggtttgacag cgacttgtgt agcataaaaa atcttcaggc gttttccttt
    43501 atctgttggt gttggattga ttgctacagc atccataatg atatcattca acacagccga
    43561 agagatacga agattctggt tcatacttac ttgttcgatc atttcaggca gacgttccaa
    43621 ccgttgttta gtcactgcag aaacaaaaac gattggagcg taatcaagat accggaattc
    43681 atcacgaatc tcttgttcga aatcacgcat tgtattggtt tctttcttta caagatccca
    43741 tttattcacg acgataataa ttccacgacc agcttcatgg gcgtaacccg ctactcgctt
    43801 gtcttgttcc cgaatccctt cttcagcatt cagtaccatc aaaacgatat cagaacggtc
    43861 aatggcacgc attgcacgca tcacgctgta tttttcagta ttttcgtata cttttccgcg
    43921 tttacgcata ccagctgtat ctatcattaa gaatttctga ccactttcag aaacgaagtg
    43981 ggtgtcgatt gcgtctcggg tcgttccttc aatatccgaa acaattacac gttcttctcc
    44041 taaaatcgca ttgatcaaag aggatttacc aacatttggg cgaccaatca aactaaactt
    44101 aatcgtgtcg tcgtcctctt cttcagtctc ttcagagaaa tacttcactg cttcatctaa
    44161 aatatcacca atccctagtc catggcttcc tgaaacagga taaggatcgc ctagccctaa
    44221 ggaataaaat tcatagatat cgtttctcat ttctggatta tctactttat tgactgctaa
    44281 aatcactggt ttattacttc tgtataaaat tttcgcaacc atctcatctg cgtctgtcac
    44341 gccttcgcgt ccgctgacta cacaaatgat tacatctgct tcttcaatcg cgatttctgc
    44401 ttgatgcttg atctgatcca taaaaggttc atcgccaaga tcaattccgc ctgtgtcgat
    44461 cacgctgaat tctcgaccta accattcacc ttttgcataa atacgatcac gagttactcc
    44521 aggagtatct tcgacaatcg agatacgctc accagcgatg cggttaaaaa tcgtagactt
    44581 accgacgttc gggcggccaa cgattgcaat tgttggattt gccatattat ttcctcctca
    44641 caagtttcca atctcactta gtttaacgaa tgcattgcca actttcaagc atagattgtc
    44701 aattaaacga agtattttta ttccttattg aaatactaat cgcttttcaa aagaaaaact
    44761 gagacaaaag cagctgcttt catctcagtc acagatacaa aatcaattat ttttcagaat
    44821 catcggttgc tttttcttct acttcttctt tcaacgcatc acctaaaatg tctcccatag
    44881 taaatccagt attttcttct ggtaattcat aagactcaac attttttggt tcttcttctg
    44941 aagctggttt ttcttctaaa gctttgatac tcaatgcaat acgatgttct tctggatgga
    45001 tctctaatac tttcactttc acttcgtcac cttcatgaag cacttcatgt ggtgtcgcaa
    45061 tatgtttatg tgaaatttga gagatatgga ctagcccttc tactccaggg aacacttcaa
    45121 caaaggcacc gaagcttgtc aaacgtttaa ctgttccgtc taatactgtg ccaactggag
    45181 cttttgtatc gatttctgac caaggtccag gctgtgtgtc tttgattgac aaggaaacac
    45241 gttcttgttc aggattgata gacaacactt tgacatttac atcatcattg acagccaata
    45301 catcacttgg ttttgctaca tgactatgtg aaatctctga gacatggact agaccgtcaa
    45361 ttccgcctag gtcgacaaat gcgccaaaat cagtaagacg tgcaacacgt ccactgagaa
    45421 catctccctc atggatagtt gaaagaattt cttttttctt ttcttctctt tcgttttcca
    45481 ctaccgcttt atgcgataag atcaaacgat tttctgatgg ttcgatttcg atgattttaa
    45541 agctcaatgt tttgccttta tagtctgaaa agtctgcaac aaaatgatct tccaccattg
    45601 atgcagggac aaaaccacgt acaccgacgt caacgactaa accgcctttg acaacatttg
    45661 taacaggtgc ttcgatgacc ttgccctctt ggaaatctct ttcgatatct tcccaaactt
    45721 ttttagcatc taaacggcgt tttgaaagta gatagctgcc gttttcttta tctttgccga
    45781 tcgtactgat aacgactaaa tctaatacat caccgacttt tactacttcg ctgatatctt
    45841 ctaccggcaa agttgataat tcttttgccg gaacgactcc ttcaactcca gccccttcga
    45901 ttccgacaat cacttgtttg tcttcaatga ctaaaacttc gcccttaaca acatcgccaa
    45961 cttttacttc ttgtacgctt ttcattgcgt cttccattga ctggttgttc tcttgatttt
    46021 gatcaaattc tgtcataaca cttgtcctcc taaccaacat tctgccgtaa gtacggtact
    46081 gtttttagtt taattgatta ttcaaaaaaa ataaagcgat ttcgttcgtt ttccttgaaa
    46141 aaacaattgt ttttcatttt ttttcttaat ttaaaaaaga agataacctt ttttcaaaac
    46201 aacagctttt attgctgcaa cgacttctgg aatcgatttg ccagtcgtat cgatacgaac
    46261 agcatcctct gcttgcttca gcggagagac gtcccttgtt gaatcataat ggtctctttc
    46321 ctgtatctct ttcgtcagtg tttcaagatc agtcggaatt cctttttctt ggttctcttt
    46381 gaaacgtctt tctgcacgct cacttacgct tgctactaaa aagattttca cttctgcatt
    46441 cggtaataca gctgtcccga tgtctcttcc gtccatgaca acgccaccgc gttcccctat
    46501 ttttcgttgg acagcaacta atttttctct gacttttcca tgtgcagaga cttcagaaac
    46561 agcttttgtg acatctggtt gacggatttc taaagtcaca tcttttgatg ctatgaatac
    46621 atgctgtcca tcttcttgtt gcgaaaaaga gataggatac tttttgatca gcgaaactag
    46681 cccttcttca tctgaaaaag atacattgtt cgtaatagct aaataagtta ctgcgcgata
    46741 catggcaccg gtatctgtat aaatataatt caattctttt gctaatatct tggcaacggt
    46801 acttttccca gatgaagcag gcccgtcgat tgcaatactg atttgtgtca tgatataact
    46861 cctttttctc agaaaaaaag ccgcaaagcg cggctattat tatttaattc ttaattcttg
    46921 accagggtaa agcataaagt tgtttggatc taagccattc aattcaaaca attgatcagt
    46981 ggtgatccct gcacgctcag ccacttgttt tggaccttcg cctgcttgaa cagtcgttgt
    47041 tgaaccggct gcagctgatg aagaaggtgt tgtttctgct gcattgctgc ttgaatcccc
    47101 tgcttgataa gttgcctcac ttgaggaagt cggtgttgtc tctgctgcaa tactgctttc
    47161 tgacgttaca cctgtatctt gagacacagt agattcttcc gcgattgtac tagattctac
    47221 tgttgaagtc tcagttgact cctcttttgt tgtacttgta gaactttcta ctactgatgt
    47281 cgaagtttca gaagaagttg ctgctgttgc attgttgctt gatccatttc tgatccagaa
    47341 ataagcacct gctggaatag cgatacataa gaccagtaaa atcaaaaata gcgaaagaaa
    47401 taatgtattt cctttctttt gttgtctctg agaacttctt gatgaaactt catcatctgt
    47461 atcgtagatt ggttgttccc atggttcttg tgtttcgtta ttttctgtag attgatgtct
    47521 atcttttctg ctcactgttt tacctcctaa actctatcgt gtgtattata ccataggaaa
    47581 aagtgtcttg tcctaaaaaa tcaatgttcc atgttttttt taaataaatt tagtaaaatc
    47641 gttccccaat gaagttgttc agtcttagaa aaaagcttcc tctttggcag aagttctgct
    47701 tttgctccat caatatcaca acaattctgc ggcttttcgg agagtttttc gccaaaataa
    47761 gctaagatga attttctgcg gcagttttct tcattaatat agcgaagcat ctgctgcagg
    47821 cggaattctt tttctttttc ctgacgtttg agccgttcca cccaattttc tggtttttca
    47881 gactgctgga tcagctccat ccattttttt tgcagctcat caaagggagc ttgctctgtg
    47941 gcatattcca aataaagctc aaaactttgt cgctgttctc tagaaagttg attaaagaaa
    48001 taatggatac gttcatcccc actttgataa aaaagaattg ctgcactttc ttcctgatct
    48061 ctgcctgctc gaccgatttc ttgtacatag ttttctagac tgtctggcaa atcataatgg
    48121 accacataac ggatatcaga tttatcgatc cccataccaa atgcattcgt agcaatgaga
    48181 aactgcagct gattttttac aaactgctgt tgaagttgtc ttctttgagc tgcatcaaga
    48241 ccaccgtgat agtatccaac tgtaaaacgt tccctgaata aatgatacaa tcgttcgact
    48301 tctttttttg ttgcacaata aatgattgcc gctccttgtg ctctttccat gaagtgttca
    48361 agttcttgtt ctttttgctg cgttttccga acaaaaagcg agatattttg ccgattgact
    48421 gattgtctga tctcttttgg tggtcgatca aatagtacat gacgtatatc tcttgctact
    48481 aaatctgttg ctgtcgccgt tagtgccagt gtgaccgggt ttcctaaaag ctttcggatt
    48541 tttcctaact gttgatattc tggacggaaa tcaactcccc attgagacac gcaatgagcc
    48601 tcatctacta catataacgc aatattttgt tttttcagat gctctaaaag agaaggatta
    48661 gtcaacattt cgggactcaa aaacaaaaat ttatattgcg atagatgacg gaggacgtat
    48721 cttctttcag attgcgaaag taaactattg aaagcagcta ctcgtttctc ccctcgtttt
    48781 tgtaattgcg tcacttgatc ttccattaga gaaagcagcg gtgacacgat gatgaccaga
    48841 ccttccatta aataccccgt caattgatag caaaggcttt ttcccgttcc tgtaggcaaa
    48901 atagcgagag tatcttctcc atcaagcaac gcctgaataa tttcttcttg gccgggacga
    48961 aatgaatcaa atccaaaata tttttttaat tcatttatta acattctgta tcgcttcctt
    49021 tctcaataga tagatttgat acaaacgaaa ttctccataa tctaattgtc catctacatt
    49081 ccattcgcta aagcgccact ctcgcacgga atccttattt ggttccaaac gttcgattgt
    49141 ttctgcagag agaatccact cgtatggaaa atcatcaaaa aacaaggccc actcaataag
    49201 atgatctgtt actgttcctc tttttaaatg acggattgcc atgatctggt caattgtctg
    49261 accattcata aacatctgct tggtgatcat catgctttga ttgaaatttt gctgtaataa
    49321 tgaatcgatc aaagcgtgcc aaagcgaatc tggccgtttt tcgatttgtg ctaacaaaag
    49381 gtggatacat ctgctttgtt gcagataaac cgctgactca tcattgtttt ttgccagctg
    49441 ataaggaagc agccctgttc gctcatttcc agataattgg ttagctaaaa aatctgctgc
    49501 ttcttctggt atctccgaaa acagctgcac caaactctca tacgcagtat caatcaaaat
    49561 ttcacgtggc aattggcttt cactaagcca tttttttagt tggaaagtat aaaaaggact
    49621 tgtctctgct ggaagatagt cttgaatacc cgttgccaga ttagaaatta cttgaacagc
    49681 aaatttaatg agccgccaac tagtttcccc tgttctgcca tagcggtcaa aacgcaaacc
    49741 agtatgttct atttgatgtt cggacaacat atcagcccct gcaggcgtta gctttgcttc
    49801 attttcattt atttcgatcc aaccttgctg cgcaattttt tgcaaaattt gataaaactt
    49861 atcctgcttc aattctggaa aactgttatg gataaacaaa agttcgtggg taaatccata
    49921 aataagtact gaagtcgttc gttttcccac aaggagttgg taaagagaac taatccgcag
    49981 cttgtgtcct gatgaaaata gcgataaaat aaaaacaatc atattattct ccttaagtta
    50041 ggatggttag aaatgtcact actatgtaaa cttgtacctg aacgctgtat tgcatgcggt
    50101 ctttgtcaaa tcgaagcgcc agatattttc gattatgatg aagaaggaat cgttttattt
    50161 gcccaagaac cagaggctca tcaacagttc gtgatgccgg aagaagaaac atatgtaaaa
    50221 aaagcgtatc aaaaatgtcc agttcgagcg attttattag aaaataaaat ataaatataa
    50281 acgaaacttt aaaaacgtac actaagttct ctaaacttga aaataagccg ggatctgttt
    50341 ctcttgttcc gtgaaatccc gacttatttt gaaagattca tttgctatct atagttaatt
    50401 gactagtttt ttcacggacc ctataggacc catttatatt atttcaactt tttcagccag
    50461 ttttccaatg tattgacgat cacagtttgc tggtctgcgt tagaaatttc tgcctctcca
    50521 tctccttttt gctcaccata gctgccaaaa cctccatgat ttccgccatt gattgataca
    50581 tactctgttc tttccggtaa atactgctta gcttcttgat aagcttcctg attcaatact
    50641 ttatcttttg tcgccgtgat cgataaaaca ggaacagctg attctgtcaa tgttcctttt
    50701 ttgtcgggat aacttgccat gaagaatacc ccttttaaat gtttgtctga atgattctta
    50761 ttgacataac gacttgccat tacgccacct aatgaatggc caccgatcac ataatcggaa
    50821 tttggaaaat cttctttgac cttatccgct ttgtttccgc ccatcactgc tagatcaagt
    50881 ggcattttga caatagcgac tgaaaagccg ttttttgcta aatcttgcgc ccaaatacta
    50941 taactttctg gctctacgag agcaccagga taaaaaataa gtaatggaga ctttccatga
    51001 ttctttttct ctccttcaaa aaataagtaa tcttctttgt tttttccttc ttttaccaac
    51061 acttgcgctt ctgacgatgg ttcgtacgta ttttgctgta tatagtaata accggctcca
    51121 ccgagtaatc ctaaaatgat gactaagcca attagtattc gtctaaatat tttcactttc
    51181 tcccaccttt tctatctgag tgaccccttt ttcttaatca agtatgatta taccttaaaa
    51241 aaataagaga gaaatgcaaa ctttccagca tcaaataaaa aaagacggaa tatccatttg
    51301 aatatccgcc ctttttgtat gtttattttt atttagcaca taaacgatac acagcttctg
    51361 cataaatttc cattgcttta tacatactac ttaattccca tcgttcattc gcctggtgca
    51421 taaagtcagg agtatcaggg aacatcgcac cgaatgccac acactggttc atcgtacgag
    51481 caaaagtagc gccgcctgat acaaacggtt cagtcatgtc tccagtaata tcgcgatatg
    51541 tccccaatag atttttcact aactcactgt ctagtggcac gtataatgaa tcaaggaagt
    51601 caaattcttc ataagtcaac ccatattttt ctgctgtttt agttaattta gctactaaat
    51661 catctttctt gaatgtaaca gggattctca tatctacacc gatttttgtt tgttctggtg
    51721 tgatttccaa acttgcaaag ttcatcgtta gctcgccgga ttgttcatct tctgttttgc
    51781 ccacgacagc ttcaccagtt gcattttctt gaactaattt tcctaagaaa ttgattgggc
    51841 caaaatcaaa tacttctgaa agagcaattg ccagacgaga aatagcattc actccttgtg
    51901 ctgcatcttt cgcgtggata cttttaccaa gaacgacgat gccttcacct tgttcttcga
    51961 aatcgaattc gtgttttttc aatgcttctt ttacttcaga aagcttttct ccagagtaag
    52021 gagctgcatc aggaacaaca ttcaacgctc ctccagcttt tacagaaaac tcgttcgttc
    52081 ctggtcctgt caagtaagct tgcaataagc ctttttctgc ataaattaaa ggaaattcag
    52141 catctggcgc aaatccttgc gtaattcctt cttctttttc gttatatttt tctaaacaac
    52201 gccataaatt ttcttcatct gtaccaaaga taaaacgaat acgtgtcttg aattccacgc
    52261 cagcatcaat caaagctttg acggcataca ttgccgcaat agaaggacct ttgtcatctt
    52321 gggatccgcg accaactaac cagccgtctt ttatagttgg atcgaatggt tttgtttccc
    52381 aattattttc gtcccctgct ggaacaacgt ccatatgaca caagataccg aataattcat
    52441 ctccagagcc aatctctgaa taaccgtaat agccttctgg atctttaaat gttctaaatc
    52501 ctagcttttc actgatttcc aatactttat ccaaagcttc aatcactttt ttaccgaagg
    52561 gatgtccttg tcctgaatct gcttcatcta gtacggaagg aattcggatc aattcgctca
    52621 atgattctaa tgcttgttca tgatgttgtt ctgtcacaaa ttttttcatg atattatcct
    52681 cctaatatgt taaaaagggt catgtcaaat tcctccgaca caacctcttc ttctcaaata
    52741 atgacgtttt aagcctgctc catcagcaag cagaatgttc ttaacaaatg atgaaagaaa
    52801 aaacactcac tgaatggagc tattgactta tccacgtgct tatcgatagc cgtttatcgg
    52861 tttcttcatt cttatgatag accttgtacc tgtatttgtc taggtttaga acattgctgc
    52921 tacacctagg atcacacaga tggctacaaa taatatcgca attaatttac cggtaaattt
    52981 ccaccacact gaaacatcga aacgagcaat tgctacagca cccattacga tagcagatgt
    53041 tggtgtaatt aagtttacta aaccagatgc agattgataa gcagtgatta ctaaatcctt
    53101 accgacgccg gcaaattcac ccataggtcc catgattccc attgttgcag cagctaagcc
    53161 ggaagttgaa ggaatcaaga atgacatagg gatatagaaa ataaatgtaa taatgatgaa
    53221 tacacttgaa gataatgaac ttaatccttg ttcgccccaa tgtaagatgg tgtctgtgat
    53281 taatccattg ttcataacca cttggatacc acgagcaaca gctactacga ttgctacacc
    53341 gatcaaatca gaagcaccag ctaaaaattc agagacaaat tcagactctt tcatgccata
    53401 agtgattgct actacgatgg acattactaa gaataacata gtaatttctg ggaagtacca
    53461 gtcacctaat ggaagcatgc ttttacctag aaccgtacct agtaccggaa ggtttgttaa
    53521 ccagtttgtc aatgattcaa aaatcgtgaa attcgcattc aaggttgtcc aaggaatcaa
    53581 actgatgatc atgataccaa aagtaataaa gaacaataca ttaacatgtt tttgtttttt
    53641 cgtgattact tcttgacgac cgattgcttc aatatcgaaa tgcttcatat cttctttacg
    53701 ttggttgtat actaatgatt ttgttggatc tttttcgatc tttgatgcat aacgatacac
    53761 atatacaata gaaatagcta acaaaataac gaacatcaag aatcgaagac caataccttc
    53821 acccatcgaa atcccaacag cttgtgaagc tacgcctgtt gcaaaaggat ttaccgttga
    53881 agctagacag cctacttgag accctactaa tacgattgct acagctacta ctgtatcaaa
    53941 gcctacagcc atcatgactg ggatcagtaa agcatagaat ggaatcgttt cttctgccat
    54001 tccgtaagta gagccgccta atgcaaataa cagcatcaat actggaataa gcattttttc
    54061 ttttcctttg aatttcttca caactgaagc aatcccggca tctagtgccc ctgttttgtt
    54121 gataatacct aaaaatccac caacaaccaa aatgaagaaa gagattggga ttgcagcagg
    54181 tgtttcttta gttagctccc ccgctggtac acctagcatc ccattgattg gcgccatgaa
    54241 aatatcccac agcccttgag gattttgttg tactcgttca tatgatccag cgacgatgtt
    54301 gcctgcatcg tctacactat agtgcccagc tggtataaac caagtaagta cagcgattac
    54361 tgcaataata ataaataaga ccgtatacgc tgaaggcatt tgaaattttt tcttttttgt
    54421 ttgttccatc ttcttcttcc tttcttttcc gttagagaca acaattttgg ctctaatgtt
    54481 attttatcaa aaagagtatc agaacaaaaa taataacacg cattttgtaa aacttttgtt
    54541 caaaaagttc gtttttccct attccctcct cttttttcag gtttttaaag ctttcttctt
    54601 tttcattgta gcgtttcctt ttttttataa acaaacattt tcgtctttta aaaggagaag
    54661 aatttcacat ttgcaaagaa ttgattgatt tttatgcata aatcaaaaaa atactcaaaa
    54721 caaacaaata aaacgtttac attattgcat gtcttgtatt tttttccaaa aaaaaagacc
    54781 aagaaatttc ttgatctttt tgatatttat ttgactgctg tatgacgttg ttcgaactga
    54841 tgttgttttc gacttaacca tggaagaagt tgcgcatgca gcactaaaaa gacagcactc
    54901 acgatgaatc ctttgatcaa gttgaaagga acgatcccaa tcagtacata gtttgctaaa
    54961 gacatgccca gcatctgtgt tgcgttcagc cctaaaaaca ttaagtaaag aggcgtaata
    55021 acgaaataat ttgcgatact cataaaaata gttagagcga tggttcctgt aatgacacct
    55081 aaaaatttgt ttcttccttt atgctgtttc tttacactaa agaaataata aatcgggaat
    55141 gtaaagatcg ttgttgataa gaaactagca gtgtctccta ccaaattatc cggagaaaaa
    55201 cctgtagtca gcagatgtaa tactgaacgc aagaacgctg ttgcaactcc ggccgctgga
    55261 ccgaataaga acatgctgat caataccgag atatcactaa aatcgatctt caaaaaacta
    55321 aaagccggca tgatcggaaa agcaaaatac tgtaaaacta atgcgatcgc agcaaacatc
    55381 gctactgcaa ccattttctg tacacgggtg ttcttcattt gaaatcctcc tgttaggact
    55441 cctcttcagc gaactttttg acgtttctct ccaaatgaaa aaactctatt ttccagggaa
    55501 aatagagtta gagttatttg atcaagcgtc gtcaaataag ctctatcttc tttatccaga
    55561 ctatactgtc ggtatcggaa tttcaccgat tcagctattt ttttgcaaaa taggtcgtgg
    55621 actataacca ccggtcggga atttcaccct gcccctgaag acgaacctta tataattttt
    55681 gtttacatat tcattataca tatctattca aaaaacgcaa catctaacac ttataaaaag
    55741 taagtgttca ttttgagtgg ttcagcaaac tgctgacttc ttttttgttc agttcacggt
    55801 aatccccagg acgtaggcct tgtaaagtca gatctccgta tttttcacgt tttagttttt
    55861 gcacaggaag accaactgct tgaagcatat ttttcacttg atgattacgt ccttcgtgga
    55921 tcgtcaattc cacgatactg tggtttgtct taggatccac tgataaaatt tggaatttag
    55981 ctggagatgt ttttcgtcct tcgatcttta ttccttttgt caaaggtgca agcatcgatt
    56041 ttgtagcgat cccttttacc tttgcaacgt aaactttatc cacttcatgt tttggatgtg
    56101 tcaggcgctg agcaaaatct ccatcatttg tcagtagcaa gatccccgaa gtatcgtaat
    56161 ccagtcgacc tacaggatag atacgttctt ttaccatcgg caaaagatcc gtaacaactt
    56221 ttcttccttt gtcatctgca acagcagaaa taactccttt aggtttgtac aataaatagt
    56281 aaccgtattc ttcttgataa attgggactt catccacttc cacagtgtca tgtttcccaa
    56341 cttttatccc taattccctt acaacttgtc cgtttacttt tacacgtcct gcaaggatga
    56401 attcttctgc ttttcgacga gaagtgatgc cagcgtgggc aattactttc tgcaatctct
    56461 ccattacaat tattctcctt tattcattcc atcaaagaat agatctggtg acacgtcttc
    56521 ttcaagtgaa atttccattt cttgtgtatc aggcagttct tctaatgatt tcaaaccaaa
    56581 ataatctaag aagtagccag ttgttccata taatatcggc cgacctggtc cttctactct
    56641 acctttttct tcaatcagtt tatgggcagc tagacgctgt accgctcctg aaacctgaac
    56701 tcctcgaatc tgctcgattt cagctcttgt gattggctgt ttataagcga tgattgctaa
    56761 ggtttcgacg gcggcttgag ataatgtgtt tgatgtccct tgcgcatatc gcttaagtaa
    56821 gggagccagc tcttttttag tcgttaatac ataatggctt tccgtttcaa aaatatgcaa
    56881 agctgagtgc gagttttgag tgtaagattc ttttaattct ttcactaaac ttgtgacttc
    56941 tgcttcgctg agatttaaca gtccgcttaa ttcttcatta ctgatccctt cctcgccaac
    57001 aataaaaaga agggcttcaa gttcacttaa tttcgtcatg ttcggtatct cctactgctg
    57061 gataaagcat aattgattca tagttttctt tttgttgcgc aatgatctgc ccttttttca
    57121 tcaattctag caaagccata aaagtagtga caacttcact tttagaaggt gactcaaaaa
    57181 aagaatcgaa agtacagcca tgcgcatttc tttttatacg ctcttcgata aataccattc
    57241 gttcttcgat cgttgtgtca tcgggcgtga tcgttttttc cactttttga gaaaatttct
    57301 tacgttctaa gattttctgc atggcacgga tcaaatcgat ttttttgact tgtcctcgct
    57361 tcaacggctt ttcagcatcc gctaacccat cgatgttcat aggttctttt gtgtaataaa
    57421 gacttcgttc ctctgctttt tctgtcagtc tttcagctgc atatttatat ttacggtatt
    57481 ctaaaagctg agtgaccaat tcttctcgag gatcttcttc ccataattct tcatcgtctg
    57541 tgacgaattc ttggacagga agcaatgtct tgcttttgat tgccatcaat gtagcagcca
    57601 tcactaagta ttctcctgca acagctagct cgagtgtttt cattgtatgg atataattca
    57661 tatattgctc cgtgatcgta gcgatcggta tatcatatat atcgatttcc atttgctgaa
    57721 tcagatgaag cagcagatcg agaggaccct cgaatacatc taatttaata ttgatctccg
    57781 tcaactcatt cactctttct tctttttctt tgctagccat tttccaatgt aaggctctgt
    57841 gatttcagtt gcacggatgg agacatccgg gtacttttcc tgtaattcat ctaacatttg
    57901 aaagcccata ttctcaccgc gataagacgg attcaaagaa atgtcatgga tgatcagctg
    57961 atcgttcgtt gcccagtaaa taccaataat cccttgaatg ttggttgaac cttctggcgt
    58021 aaagaaaaaa agttcataat cgtcttcaga ttcgtacgta tcaatctcct tcaacaaaga
    58081 agttttacct gtcagtttct tatggaaact caatagtccc atagcaattt tacggttttc
    58141 tttccggtaa tgtgttaaca tcttctcacc ttcctatgac taagcccttg gaaaatgctg
    58201 cttataaaca tccgtcattc ttttcttcgt gatatgtgta taaatctgag tcgtagagat
    58261 atccgcgtga cctaacaatt cctgtactgt ccgcaaatca gctccatttt ctagcagatg
    58321 tgtggcaaag ctatgccgca gcgtatgtgg tgttacttct ttgtaaatcc cagcctctcg
    58381 tactaactgc ttcagatttt tccagattcc ttgccgagaa agacctttcc catgattatt
    58441 gacaaataca tgcatttcag atgcgtcctt tacaagtaat ggacgtgctt cgtctaagta
    58501 gcgttccagc cattggatcg cataatcacc taaaggaatg atccgctctt tatctccttt
    58561 tccaactgtc tgtaccagtc caagagaaag atgaagatct cccaatttca atccaatcaa
    58621 ttcactgacg cgcatcccag tagcatacat gacttccaag attgcacgat ctcttatccc
    58681 taacgttttt gtcgtatcag gtgcttcaat cagcttttct acttctgtca gagtcaatgt
    58741 acttggtaat ttctgtgctt tcttcggcgt atcgatatgt tgcatcggat catgatcagt
    58801 gatacgctct tgccgtaaaa actgatggaa tcttctgaga cttgagatca tccgagtaac
    58861 agttgctgtc gctttttttt cttcatgcaa cttctccaga aaacggatga ctaaaaagcg
    58921 atcgacatcc tgccatgaag caattttctg ctcctgtaga aaatgcaagt actgttccaa
    58981 atcacgttca tagctttgtc gtgtattggc ggacaatccc cgttcaatat tcaagtagtg
    59041 gagataatcg ttgatttgct ctttcataat aatccccctc atgcctctct atcatatcag
    59101 ataccgcttc attctgatag tctttttcca taatctgagc aaagaaaaag ccgtctagac
    59161 actcagacgg actggtttct tttttctttt tcatagcatt cctgacagat tccatggaac
    59221 gttagccgat gatcttttac taaaaaatga taacgttctt ctactacttg ctccacttca
    59281 agtaaaagat cctcttctac ttcctcgatc gtgccgcagt tcaagcatag taaatgatga
    59341 tggaaatgtt tggcgccttc ttttctcaaa tcataacggg ctaatccatc attaaaactt
    59401 actttatcga caattttcaa ttctgtcaag atttcaaggg ttcgataaac agtagcaagt
    59461 ccaatttccg gactcttttt tttcactaaa aagaagattt cttctgcaga taaatgctct
    59521 ttctcatttt ccaaaagcac aagcagagta gcttctcttt gcggtgtcag cttaaacccc
    59581 gcttcatgta gctgctgttt tgtttttttc atcgccgaaa tagcattcat gaccatttac
    59641 tcaaccccaa caatttataa taattacaaa taaaaaaaga tataacacta tgttaattaa
    59701 aattattata agctagttaa gaaaaacatg caagggattc tcaattagat tttttgacta
    59761 gttttgtcac accgttttct tgtgtaatca atccttgttt caaaagatta cctaatgcgc
    59821 gcttaaatgc tcctttgctg ataccaaaag ctttcatgat ttcttctgga tccgatttat
    59881 ctgtaaagtt gatttgctga tctgcagaac gttctaaaat ggttaaaatc atttgtgcat
    59941 catctgatat agcttcatat gctcttggct tcaatgaaag attcagtaca ccgtctggtc
    60001 ggacaccaat cactcgacct tttatctgct cgcctagacg aggttcatta tatcgttcag
    60061 aaggatggat aaacccaaga tagaaatctt ccgttaaaac gtacgtacct gttaatttca
    60121 agcggtatgc tgttcctatc acatctttgt ttttcatttc ttctgttcca tatttgctta
    60181 aagatttgaa tattttttca tctgctaaac tgccccaaat acggtctttt gcatcaactc
    60241 taagggaaat catcaagcga tccccttttt taggccatag ctctgtcatt gtcggcaatt
    60301 catcaagaga tacgacaaag tctttatctg gaagaccgat atcgacaaac gcacctaaat
    60361 cttttcgtgt agcagtaacg attccaaaag cgtaatgacc gatacggctt ttaggaattt
    60421 gtgtagtgat ccgattttca tgtttttgat tttggtaacc gaatccttca actgcttcac
    60481 cgattttgtg ttcaccttct gatttgctta agccaaaggt ttgtccgttt ttttggacaa
    60541 agtagttttt ttcattttca tcaataataa tacctgtaaa tatagttgct aataaatcgt
    60601 tcattttttt ctccctctat agtgaaaggt tttcttaata catttctttg ctatcataac
    60661 ataatgttat ctacttgaat agctgagcct tggtttggaa aggtgaaaaa aggagtatga
    60721 caacagttgt gtcatactcc cctttttcga ataaacggtg ttaaacggca ggctcacagc
    60781 ctctttcatt tgcctaaaat taattgctcc catttcctcc gaaccatcca gttttacagt
    60841 tgatggttac gatgtaaaat ggcaaagcac tttctccaat gcctttcatc aatgcagatt
    60901 ttgttgaagt ggcatataga ttataatagc cttccccatt ttcgattctg gctttttcta
    60961 atctgtaacc tccttcaacg atatatccac gttggataca tgtagcaatc actttgcttt
    61021 ggacacctgg tgcccacgtc catgctggat tagatgcatc ggcaatatca cttgctctgg
    61081 cagatttgat tggctgattc gctgagtttg aagaggaagg ggtcgaggat gccgcttctt
    61141 gctgtgctgc ttgtgcgacc gcagcttgtt gagcggcttg agctgcagct gctgcttgag
    61201 cggcttgttt tgcttgttct tcagctgctt ttttcgcttc ttctgctttt ttcttttcct
    61261 cttcttcttt tttctcttta tcagtgagca tcttatctac ttgtttcaat tgttcattca
    61321 actgagcaga tagatctgta tttttcactt tttctactgc ttcttttgcc gtcttgtatt
    61381 ggtcgcgtgt tgctgaatcc actacttttc cgtctttaaa gactatagct gttttttctt
    61441 tagctgtttg caattcagca tattgatttt tagcttctgt ttgtgcttgt tgaatcaatt
    61501 ttccaaaatc tgtgttatcc gtttcgattg cttggatctc tttatctgct ttcaacttga
    61561 tgtcatctgc tagatgatca tcgacgatgg caggtgctat gaaccactca ttcacttttt
    61621 ggatatctcg gatcttagat tggatatctt ggtaagttgt ttctaactct gaatatcctt
    61681 tttcattctt aagactggat aaatcatttt ttagttttga taaatcttga tgaatcatat
    61741 ctgcacgaat aaattgatgg tgatcatctg tataaaaggc agcaagtttc tcatgaagtt
    61801 cttccatttg ttgctgtctt tttgcttctg ctgctacttc ggcttctact ttttgatgat
    61861 ggtcataata agcccagctt cctccaccga gtagaagtac tgctgctaaa acgagataag
    61921 gtactttttt tgattttttc ttttgcgctt tatctggatc tgttttttct aagtttgatt
    61981 cttgatgtgt tttttcagta tctggcacga ttgttattga tttcgcttct gcttcaaaca
    62041 gcttaggctg gttctcttct ttttctattt ttgcttcaag atcagtaccc tctttgctta
    62101 cagattgatt ttcagcatcg gattcactat ttatttcatc gggctctgtt gactttttgt
    62161 ctgtgttttc gagtatttct tttttctctg cttccggcag tttattcgtt ttttcctttt
    62221 ctaattgttt ttcagtaaat acttcagttt tttctgtgat atccgttttt acgtctttta
    62281 tttttactgt ttctttacct gtggcgtttt ctctatcaga gtctttttca gtttcttgtt
    62341 ccttttctgg ctctactttg ttttcctgtg caattttttc actgatcatt ttgagcagct
    62401 ctttcgcgtg ctcttctttt gtatcttcac gatgtgcttt gatataagcg gatagtatcg
    62461 aattttcttc ctcattatcg acgatttctt tcagttcagc tactcttctt ttttgttttg
    62521 attcaagcgt ttcttcttga ttctttcctg attcttccga agtctcatga acagttgctt
    62581 tcttctgttc atttttctct atattagtta cttgatcgtg agcaggtggt tcttcgtctt
    62641 ctggttctgc tttttcgaat aattccatta cagattctag aggtaaatct ttcaattccg
    62701 accattcgat tggatcgttt aattctgtat caggaaagac tgcatcttca gttgatttat
    62761 cctgcatagc tgcatctgta tttgtttctt caattgattc ttttatttgc tgatcttctg
    62821 cagttatttg atgaccgcag tctggacaga tagtattctg ctccattttt ttcgatccgc
    62881 aatttggaca ttttttcatc acttagcgat cggctccttt actaggttta cgctaccatt
    62941 taatctatca tattctatac gtcacgccaa tagcttattt ttaggattta cttaaaaata
    63001 atgaatgaat ttccttccta tagaaaaaaa agcttgagac tgcgtagtct caagcttttt
    63061 ttcttttaaa ataaaccaaa cagttttact gatttctctt ctggtcaata gtccacgctt
    63121 attctaagaa atcttttaat tgttttgaac gagaagaatg gcgcaatttt cttaatgctt
    63181 tagcctcgat ctgacgaata cgttcccgag taacaccaaa tactttccct acttcttcca
    63241 atgttctcgt ccggccatca tctaaaccaa aacgtaagcg aaggacattc tcttcccgat
    63301 cagtcaatgt atctagtaca tcttccaatt gttcttttag taactcataa gctgcgtgtt
    63361 ctgctggact tgttgcttcc tggtcttcta tgaaatcacc taagtgagag tcatcttctt
    63421 caccgattgg cgtttcaagg gaaacacgtt cttgcgcgat tttcaagatt tctcggactt
    63481 tttctgttgg aagatccatt tccgcaccga tttcttctgg cgtaggttct cttccaaggt
    63541 cttgcagcaa ttgacgttgg atacgtatca atttattaat tgtttcaacc atatgaactg
    63601 gaatacggat cgtacgtgct tggtcagcga ttgctcgagt gatggcttga cgaatccacc
    63661 atgtagcata ggttgaaaac ttaaatcctt tgcggtagtc aaatttttca actgccttca
    63721 tcagtcccat gtttccttct tggatcaagt caaggaactg catgcctcgt cctacataac
    63781 gtttagcgat acttactact aaacgaaggt ttgcttctgc cagacgctgt ttcgcttctt
    63841 ggtcgccttc ttcgattttc aatgctaacg ctacttcttc ttccgcagtt aaaagggaga
    63901 cacggccaat ttctttcaga tacatacgaa ccgggtcatt gatttttaca ccggttggcg
    63961 cagaaagatc ttctgttttt gcttgttcag cttttttctc agcactcttc aagctgtgaa
    64021 ttgatggatc accattttca tccaccacac tgatacctgc gtcttcaatc ttttggatca
    64081 atttgtccat ttcatctgca ttcaaagtga atggtgtagc aagctgattg gataaatcat
    64141 catatacaac ttgtcgtttg ggtttgtttt ctttgatgaa agctgctact gctgcttcat
    64201 acgttttttt atctgttcca tttgccataa aagaaggccc cccttgagta gctatttggc
    64261 ttgttttaac tgtttcgtta aattgatgat ttcaatcgtc aattcaagct caagctgttt
    64321 gtttccagtt cgtctggctt cttgctgcat ttgttttttt aatgttattt catcagctaa
    64381 gccagatttg ctgatcacat ctagcaagtc ttggatttct cgtggcgaac tttcttctgc
    64441 caccggtaaa tattcgattt ccaccaccaa ccgccttaaa ttttcatctt gcaaaaagtc
    64501 taaaaactct gcaaggttga attcataatg ttgtgagaca tatgcatcga ataaaaaata
    64561 aacttctgca tatgcatcat gaataaactg aaattctcgt tcttttaaaa tattacggac
    64621 agaaacttct ttaaatgcac gatataatag aatctgctct gccttttcta cttgcgtcaa
    64681 aggtctcttt ttccgtgcag tttcttgtct cgtaacctga gtgtgttgac tttcctgagg
    64741 ttcttgacga ttttgctgat ttgtccttcg aagatcgcgc agttgttttt gtatcgtctc
    64801 tctgccgatc tgaaactctg ttgacagttg agatagatac atatcttgtt cgattggtga
    64861 aacaactcgg ctaagttcaa ctagtaattc ttcgaggtat tcgatcttgt ctttttcatt
    64921 atctaaattt ttcccttgtt ttaaataacg tgatttaaaa gtaaagactg tttcgcgccc
    64981 atgagccact aattcttgga aagatgcttc tccatactta cggagatatt catcaggatc
    65041 aagcttttct ggaacgacta cgacagaaag tgctaatcga ctgttttgcc gaagcaattc
    65101 aatccctcga ttggttgctt caaaacctgc gttgtctccg tcatagcaaa agaccaattc
    65161 tttcgtcagc ttttccattt gttggatctg ttgagctgtt agacttgtcc ccatagaagc
    65221 taatccagtt ttgataccag cctgccatgc agcaatcaca tccataaacc cttcgaacaa
    65281 caaaatttct ccgtttttcc ggatatcgct tcttgcttta tcaagattaa ataaaacttg
    65341 tcttttattg aacagttctg tttctggact attcaagtat ttgggctgat ctttttcctg
    65401 ccctttttca ggtgcaagcc agcgtcctga aaaaccgatc gtctggcctt gagaattacg
    65461 aagtggaaac atcactcttt gataaaaacg atcagacata cggccatcat catgcgtgac
    65521 aaacaatcca gagcgttcta acaattcatt tgaaattgat tcattcttga atacttgctg
    65581 aagaaaagaa cgctcattag gtgcaaaacc tatgttgaat tcttcaatta attctaaaga
    65641 caacccacga ctttgcaaat attctaaagc tggctgtcca gcttttgtat gcaatagcat
    65701 gtgatgatag atttccgctg ctttttcatg agcagcaatc agtttccccg tagcagattc
    65761 aggattttgg acagctatag tacgctgacg ccattgatct tctaacggta tctgttcgaa
    65821 gtctgccact tttaccactg cttctggaaa acttagacct tctagttctt gaagaaaagt
    65881 aaacacgttt ccgcctttac cacaaccaaa gcaatgaaaa atctgcttgt cttctgcaac
    65941 agaaaaagac ggtgtacgtt cttcatgaaa aggacacagc cccaagtaat ttttaccaga
    66001 ctttttcaac tggacatatt gtccaatcac ctcaacgata ttcgtttgac tgcgtactgt
    66061 ttcgataacc tcttgaggaa tccgttgagc cattttttca ccccctgctc tattgatatc
    66121 ttttgggttt aacagaagat atttcaggag aataaccggt aaaaaatcgc actaactgaa
    66181 atcagtgcga ttttaaagga cacaattata catgtttgat tgtatcatat ttcattgctt
    66241 tttcaagtat aacacatttc tatctcatta agcatcaatt tgctactctt ttcagtaact
    66301 gattttgaat atcagccttc cagtacaata ctcgaagaac aatatttacg agcatgaata
    66361 caaagaagta tcctacgctc tctcccaacc ctaagtttac ttccaaaatt ccatggaaca
    66421 gcattgcagc tacatatatc cctacgatcg tcgttactgc taggaagatg cgcaatgggt
    66481 tgaagggata acaagctttt acaactgcca tgcaactgat cccaatcagc aagtaataca
    66541 tcaaagtagt agtttcagca tttccccatg cgaatgtttt tccaagtaga tacaccgcaa
    66601 tgatattcac tactacaagc aaggcatttg gtaaagcatt aataagtgct gtaggcaaat
    66661 aacggtaagt gatttttctt ttatcttgtt caaaagataa aaagaaggaa ggataccctt
    66721 caatcgctaa atcaatcagc gtgatttgga tagggataaa cggaaatcca attgctgtaa
    66781 cggcacatat aatcgataaa ataaatgaat aaatcgtttt gataaaaaag atgcctgaga
    66841 ctttcgtcac attgttgacc actcggcgtc cttcaaacag gacttctggc aatgtagtaa
    66901 aatcagagtc caacaatacc agattcgcaa tctgacgtgt tgctccatca ccttctgcca
    66961 tagcaatgct gcaatctgct tctcttaacg ccaaaacatc attcacaccg tcgcctgtca
    67021 ttgcaaccgt tctgccgctt tctttcaatt catttaccag caatttcttt tgctgcggac
    67081 tcactcgtcc aaaaaccgta tagcgatgag cagcttctcg cacttccatt tcttcagtaa
    67141 tttgagaaag atcgacatac gcatcataat tcggtaatcc tgcacgtcta gcgatattgg
    67201 agacagtaaa aggattgtct ccagaaatca ctttcagatc cacaccttcc tcatgaagat
    67261 aagctagtgt ttcttgagcg ttttttcgga ttggatcatc gatttcaaac agagcaaccg
    67321 gctcaacaaa tgctggtttt tcttcgttca attgctgatt tggagcgatc cccaacataa
    67381 gtacgcgata gccattcgct tgggctgttt ttagactgtc cggaagccga tcttcttcca
    67441 ccaaacgttc aggcgctcct acgtacacga ctcccagttc tggaaattcc atcgcgcccc
    67501 atttgcgatc agaagaaaat gcgactttgt ttgtcacatc ataccgatta gaaagctcaa
    67561 aataatctct catagcccgc atagtgatgt tgttatcact gctttctgcc agatacgtcc
    67621 ccataatttc tgggatggct ttctcgtaca gcggatgtag tgcttctacc cgctggatac
    67681 tcattttccc ttcagtaatg gtccctgttt tatccaagca taatgtatcg acatgggcca
    67741 gcgtttcaat tgaatacatg tcttgaacaa ggattcgttt ttttgccagt ttggttaccc
    67801 ctgtcgttaa tgcaatactt attagcaata ccaaaccctt tggaagcatg cctaacaaag
    67861 ctgctgcaga tgcaataaca gaaattttga tagaagcatc gcggatgaac agtgcttcca
    67921 aaaatagtat gatccccaaa ggaataatca cgaaactagt gaatttggat actttgcgaa
    67981 tcgaatctac aagttcagaa cgaatagctt tgtgagtctt tgcctcgcta gtcaagcgga
    68041 cagcataatt atctgcacct actcggataa cttcagccat gacagaaccg cttgtcaaaa
    68101 agcttccaga aagcaattca gctccagttt ctttgacaat caaatcagat tcgcctgtca
    68161 aaagagcttc atttgcttct gcccgtccag atataacctg taaatcggct ggtatctgtt
    68221 ctcctgctcc gatttttacc aaatctccta ataccagctc tttagtagaa atcgattgtt
    68281 ctttaccgtc ccggatcact agtacttctg attgggaaac aatcgataat ttccgtacaa
    68341 gatttcttgc atgtatttct tgataaattc caataacgat attgacgaca ataattgcta
    68401 aatacgccat gttggaatac gcacctacaa ataacagaca aatcccaata ccaaagttca
    68461 gaaaattgaa taacgtcaat atattatcgc tgaatatttg ttttgtactt tttgatgcat
    68521 cttcctggta gtcattttgc tggccagaag ccattttctg aactacctct tgctgtgtaa
    68581 gtccattcat tttctttttc tccattttcc gccaactttc aaaaataatc acgtgtttaa
    68641 gtctagcgtg ttccaagatt aaaaacaact tgtttttata aaatttatag aaaaacacac
    68701 gaatgatttt acaaaaaaga tgccaacctt tttgtttgaa tcacccgtgt gctgttttta
    68761 ttatcaatct atctttactt atattgttcc tccaaacggc tcatccacca acctagtgct
    68821 gtaccggcta taaaaagaac aggacaagct agtaccccag caactcccaa tcccgcgtaa
    68881 tcggtaggaa tgatcataat cgttgaagca aaaactacac ctaaaatgaa atggaatagc
    68941 ttgctatatg cctttgagaa aatgtaatcc atgatttttg acaatccaag aacacaaaga
    69001 atcccaccaa ttccgatcgg cacaatcact tcaagtcgta cttctttaaa tccgtcagcc
    69061 atcgctttat acaatcccat atacaccaaa aaattagaag ggctcaatcc tgggacgatc
    69121 atacccaaac caatcaatcc gccggcaatc agccatgttc caaaattctg atcgacttgc
    69181 ccgtcaaata attgagcgcc gtataataaa aacaaataag ccagaacaaa agtaacaacc
    69241 atgatgatca catcagaagt atctcttcct tttttccctg cttctttcca taacgcagga
    69301 acagttccag cgatacatcc gacaaaaaac cataatatcg ttgtttcata cgcacctaat
    69361 aaaaaactga ctaaaaaaga aagtagaaag atgcctgtca ctccgcctag accaacaggc
    69421 agaaaaaaca aaacattttc tttgaaattt tttgtaaggt gggctaaaaa agaaatcagc
    69481 cgctcataca tgccaaagac agctgctaat gcaccaccgc tgactcctgg caagataaat
    69541 cctgaaccga taaacattcc cttcacaaac cgtaacatcc agtctttggt tgtgtacccc
    69601 acttcttgat tttccatttt ttgccttttt tccactattt gcgtactcct tctttcattt
    69661 aattttattc atgattgaac atttcctgct attttactta aagacttttt cataggtctc
    69721 tattattttt tcattcattt tgacaatatc aggataaaac atgttacctc cattatgata
    69781 gagatagccg ccattgtaaa gcaaggactg gatccgccaa tatcggtatt gtttcccttc
    69841 ctcatttcct aaaagtgggc tcagcacatc tttagaatac gcttctgcta attctactgt
    69901 gttttctccg ccatgatttt ttacataggc aatataatct ttcccaaaat tatatgcttg
    69961 gacagctgtc cataaatcgc atccttgttt ctttgcttct actatcattt ccgccaaata
    70021 tttgacacct tgatcaatac tgtcagctgg attatcaatg atctcagctt ctccatgcag
    70081 actttccgag ctctgcatga gatctgtccc ttttcctttt gattcggtca atattatcgc
    70141 cttgatcaaa ttctcgtagt tttccatatg ataatttttt gcagctgttg ttatttctat
    70201 ttgatagctt tccactcgtt ttaccgaacg ataagttctt cctcataaaa acaacaccgc
    70261 accaataacc gtcagaatga tcatgtaaaa aagaatacgg ataatagata atcgttttcg
    70321 cttcatttca tcacctagtt attttgacat ctttttttat cttactattt ctttatcttc
    70381 ttggctattt cttccttgat ttctgtgtac gccttaagaa aaaatgaatt ttcctgtatt
    70441 ttttgacatc ttacgaaaat gatcgttgtt tttcagtaaa aacaattgcc caatgactga
    70501 tataatcgtt atactttaca cagtaggtga ttatgtgaaa aaagaaaaga aatatgcaaa
    70561 aatgccagat gggagcgaaa tttactatga aaagagcggt caaggcttcc ctctcttttt
    70621 actgcatggc aatgacggca gcggccgctt tttttcagaa caggtccctg tactcgaacg
    70681 ctactatacc gtgtatctag tagatagccg aggacatggt cgttcgacaa acgaagcatc
    70741 tatgttgaat tttcagttaa tggccgagga tctgaatacg atcatgttgc tggaaaaaat
    70801 cgatcaggca gattttcttg gatttagtga tggagcaaat ctagcactgg tatttgccag
    70861 cagcttccct aaaaaagttc atcgattgat tttaaattct ggaaacacgc ttgttaaagg
    70921 cgttcgtttt tctgccagag tcatcagtaa tattcattat gcatgggtat ggttactctc
    70981 tttatttcgc ccaagtcttc gcaaaaatct tttagttatc aaacttttgc tgcatgacat
    71041 cgggcttaca gaaaatgatt taaaaaaaat caactccccc actttgatca tcgtgggaaa
    71101 aaaagatgta atcaagctaa aacattcttt gtatatagct aaaactatac caaaagcttc
    71161 ctttgtgctt gtgaaagaac aaggacatga attagctaga aaagatcctg aacgcttcaa
    71221 tcgagaagtt cttcaatttc tttctgaaac ataactatta ggaatagaag gtgatttatt
    71281 tgttaaaaaa taccatacaa tggctgaaaa accacctagg actgttcaaa acaatctttt
    71341 tgatttctgt cattgtcatt attgtcggtg aattgatgtc catcggtaaa actctatcta
    71401 tccagcaatt aggagaaacg tttgccacta tacctgtatg gaaaagcgga ctaatgctat
    71461 tgattggtct tatctctgtg ttacctatgg ttggttatga tattattttg aatcggatgc
    71521 ttgaacaaaa gcagaatcct cgttacctgt ttgagacaag ctggctgatc aatacgatca
    71581 acaatattgc cggtttcggc gggtttgtta gtgtggggct tcgctctgaa ctatatggac
    71641 aaaagaaaga gagcagaaac gtgatccaag cattatcgaa agttttcttg tttttgatgg
    71701 ctggactttc tatttacagt ctgctttctt ttttacttgt agtcttcacg ccagttgctc
    71761 cttttttgaa gcaatattgg atatggttga tcggcggcgg tctatatttt cctgtcgtct
    71821 ttttatttac tactttcaaa aagaaaggat tccttggtgg tacctcactt aaaactcaag
    71881 gacagctatt gctagtttca ttgttggaat ggaccggtgt cctgtttagt ttcctttctg
    71941 ttggttattt aatggaaatc cggattgatc tgtggcaaac aattccatta ttcattgctg
    72001 cttcagtgat aggaatcgtg tctatgattc caggagaaat tgggagtttc gatgtaatga
    72061 tgattatcgg gttgtccgct attggtgtgc caagagaaat agtcgttgtg tggatcttgc
    72121 tctatcgcct gttctactac atcattccgt ttttgattgg aatcgtcttt ttcttcaaaa
    72181 atatcggttc cacttttgat cagcgttatt ctggtatccc taaacaatta gcaacagaaa
    72241 ttgcgcataa aatcgtggtt gtcttattat acttttctgg aatcatgcta gtactttctg
    72301 ccactatccc gcaagctttt acagagtttc gctggcttca tagtttgaac ccattgaaat
    72361 ttcattttat tatccaattt cctagtatac tattgggatt tcttttaatc attatgggca
    72421 gagggatcgc agcacgtgtg aaacgagcat atttgcctac aatcttcttg attgttctag
    72481 ctttacttta tgttttatta agtgatttta gtttcacacc ggtggttttc ttgtctattc
    72541 tgcttctgat cattcttgct tctaaaaacg aattatttcg ggaacaattg atctattcat
    72601 gggagtggcg tacgatcgat ggaatcatta ttggtgcact gtccttgctt tacatcgtta
    72661 ttggggtata caatctgccg gatttccctc atcggcgtca ccattttatt tcctttttct
    72721 tatttccttc tgaaaagatt tggttttcgg gattgttggc catcatcgct gttagtttca
    72781 tgatcgttct atttgttcat ttccttcaag gagaaaagaa acagattggc gaagccttta
    72841 atgaagaaaa ggccctgaag atccttacaa cttatggagg aaactccgat agtcaattga
    72901 tcttcttaaa agataagcgg atgtttgcct atgaaaaaga cggtgaacca actgttcttc
    72961 tccaattcgc ttgcttcaat aataaatgta ttgtaatggg tgatccttct ggtaagaaag
    73021 aagattttcc agaagcaatc gaagctttta tcgaagaaac agatcgacta tgctatttac
    73081 cagttttcta cgaaacaagc gaagagattg ttatgatttt gcatgaattc ggctacgatt
    73141 tcattaagat gggggaagaa gcttatgtag acctgaatag ttttacaact tcagggaaaa
    73201 aaatgaaggg cactcgtgct gtcttgaatc ggatcgaacg agaaggattt acatttgatg
    73261 tcttacaacc accattttct actgagcaaa tgagtacttt caaaaatatc tctgataatt
    73321 ggttgggttc acgaaaggaa aaagggtttt ctttaggctt tttctctgaa gattatctac
    73381 aaagagcgcc tattgcagtt gtcaaaaata cccaagaaga aattgtcgct tttgcaacga
    73441 tcatgcctac ttacacgaac aataaagtag gaacgattga tctgatgcga tatgattcta
    73501 aaaaagcacc ttctggcagt atggatttct tgttcttgca tttgtttgcc tatatgaaag
    73561 aagaacatat ccaatggttc aatttaggga tggcccctct ttcaaatgtc ggaacttcca
    73621 gaaagagttt tttacaggaa aggattgcat acttggttta cgagtttggt tctcattttt
    73681 attctttcca tggattaaga gaatataaaa gcaagtacgc tacaaactgg gtctcacgtt
    73741 atactctata ttctagagat agttggattg catatgttat gattgctttg ctgattattg
    73801 ataatgcgcc agttgagcag acacaaggta gaataagtgg tttgaaaaaa tggatacgaa
    73861 gaaagtatta aaaactgcat accgatagat gaacactctc atgatgaata aaagagactg
    73921 aagtaaaacg aaagttcatg cttctacaat ctcttcttat acaattgaag aacaaagagc
    73981 ggtccaaaca gaaatcctgt ttggaccgct cttttatttt aaacattttt cattaccaaa
    74041 atattcgagt cgcgtttctt atgcttcgat ctctgtaacg atacctgaac ctactgtccg
    74101 tccgccttca cgaattgaga atgtcgttcc gttttcaaca gcgattggat ggatcaagtc
    74161 aacatcaatc gtcacgttgt caccaggcat gaccatttct gtatcttctg gcaatgtgat
    74221 tgttcctgta acatcagttg tgcggaagta gaactgtgga cgatagttgt tgaagaatgg
    74281 agtatgacgt ccaccttctt cttttgtcaa cacgtacact tctgctttga attttgtatg
    74341 tggagtaatg gaacctggtt tagcaagtac ttgtccacgt tcgatatcgt cacgttggat
    74401 cccccgtaac aacacgccta cgttatcccc agcttccccg taatctaacg ttttacggaa
    74461 catttctaca cctgttacaa ctgctttttg tgtttcaggt ttgatcccta cgatctctac
    74521 ctcatcaccg acacgaacag caccacgatc aatacgacct gaggcaacag ttcctcgacc
    74581 tgtaattgag aagacgtctt ccactggcaa tagtaatggt ttatctgtat cacgttctgg
    74641 tgttgggata tattcatcta ctgtatccat caattccata atagcagctt cagcatctgg
    74701 atcgccttgc aatgctttta atgcggaacc tttgatcaca ggagtgtcat cgcctgggaa
    74761 accatattcg ctcaataact cgcgaacttc catttctacc aaatcgatca attcttcatc
    74821 atcgactaaa tcaactttgt tcaagaaaac aatcaggtat tttacaccaa cttggcgtga
    74881 caataaaatg tgttcacgtg tttgaggcat cggaccgtca gttgcagaaa caactaagat
    74941 tgcaccatcc atttgtgctg cccctgtaat catgtttttc acatagtccg cgtgtcctgg
    75001 tgcatcaata tgcgcgtagt ggcgtttttc tgtttcatat tctacgtgtg cggtattgat
    75061 cgtaatccca cgttcacgtt cttctggagc tgcatcaata ctagcatagt cttgaggatt
    75121 tgctaggcct tttttgccta atactgtagt gatcgcagca gttaaagtag ttttcccatg
    75181 gtcaacgtgt ccaatcgtac caatgttaac gtgtggttta cttctgtcat aatgttcttt
    75241 tgccataatt tacaaacctc ctaaataata tagatcactg aaaaatttca gctctcacct
    75301 ttttattata gtcttttttt gactaaatca aaaagaatac gtccgactta ctttttgtaa
    75361 gattcgaact aagcttatta tacgtcaata ttggtcaaag tcaactaaga gcactcgtct
    75421 gtcttatacg cgaaaaaagt accttttata aataaaaaaa tgtgtttttt gagaaaaact
    75481 tcaagatagt ccttaacttt cttttgaagc aataagaaca aaatgaggaa aagtttaaaa
    75541 ttcagctgtt gccaacaact ttcaatagac gaaatcttct atttcgttaa taataaggaa
    75601 agaggtgaag tacatgaaaa aattaattgg cgtacttgtc gttttaggca tatttctcgg
    75661 cgaaagtttt gcagcttatc attattttta cggcggcgaa gcttattata caaaaatcac
    75721 gacgaatggc gaaatgtcag ctacccaagc agataacgga gagaaattca cgacttatac
    75781 ctacaagcaa gcggcttata ataaagatgg tgaaaaaaaa caggtgacgc ttcgcgaaga
    75841 aagagaacat ccattaaaga tgaatgcgta tcttaaactc aaagtcaatc ctcgtaaagg
    75901 tgtattaagc tgggaagaaa taaaggcttc tgaagtgcct aaaaatgcgg cagaaaaaat
    75961 cgataattag taaatggtct taaaatttta ttagataaaa agaggctggg acagaagcgt
    76021 ttaactccaa gaaataagaa ggagttgctt ctgcttccgc cgtttatacg tgtttagagc
    76081 gtgagacaaa agtgttcttt acttttgtct cacgctcttt ttttgttata ttcgtctttt
    76141 ccgatccttc tcttgacaca attcatttaa ctgatcactg gtacttgaac acctaatatt
    76201 ctagtcattt ttataaacga ttcaatactt catccacatt tttgatcatc acggatatcg
    76261 taccatccgg attattcaca aattcaatca aatctgggtt gcgatataca tctactggta
    76321 cgatcagttc gatcccgttt gatagcttga atttttgttt tccaaatttt ttctcagaaa
    76381 tttcttgtac ttcttttaca ggaggtgctt ctgggacaaa tccttgctct tgtacttctt
    76441 ctttaaaagc catttttgcc gtgatgtttt ccttgaagac tttttccgca atcatctcat
    76501 gatccaaccg acctttttct tctatcgtat catagacagc ttctttcaaa tcagccatga
    76561 tatcaaattc ttctgtctca aatttttttc caagatgctt ggctactttt tggatcactt
    76621 ttacattttc ttctaaagaa ggtgcaggaa cactttcgat cactcttcca gaaaaataaa
    76681 gctctttttt gccagaaaac tcatattttt tttcaattaa ttcgtaggtc caatcagtca
    76741 aattcacagc gaatgcttca tctgcttttt gtgttttcgc ccctagaatt gctcgattca
    76801 aaatcaagcg gttgtcgatc cctgcttcgt ctgcttcaac aaagtgagtg taagcctcac
    76861 gataattgac tttcaataaa gataagtaca tcactgtatc taattcatac aagacaacaa
    76921 atacatctgc actaggtgct tcttcacttt cttggtagat atcgaaccac cgctgaacga
    76981 ttgcttcact tgcttccaca aaattttctt gagcgatttt aagtgattca acgatcatgc
    77041 tttcttctgc tagttgtcca gtttttgttt gagcagaaga gattttttgg atcttctttt
    77101 gaagaaaatc acgaatatat tcttttgtca gatccagctc tttttgtgaa aagactggag
    77161 aaccagtttc tctgtcaata atatgcagaa ttgctttttt taaatagata tccattttct
    77221 cattcccttc tgtcttagtt tacggaaaaa aaatcaaaaa taccagaatc aaaatcattt
    77281 tcttttaaac aaaaatttca agagtagaaa caaaatggat tctttttttc gaaaaatgcg
    77341 ctacacttat agttgaacga ataaaaaagg agctgaaagt atgaattcat taacaagcac
    77401 ttatcgtcta tcaaatggtt acgaaatccc ggtagtagga ttcggcacat ggcaaacacc
    77461 tgatggggat gtcgctgttt cctctgttaa agaagcgctg gctgcaggtt atcgccatat
    77521 cgatactgcc caaggataca agaatgaaga aagcgtcggc caagcgatta aggaaagtgg
    77581 aattcctaga gaagagatat tcttgacaac aaaattatgg aatgcaaacc acagctatga
    77641 acttgtgatg tcttcttttg aagaatcatt gaagaagctc caaacagatt atttagatct
    77701 gtttttgatc cactggccaa accctgttgc ttttcgtgat aattgggaac aagcaaatgc
    77761 agatacatgg cgagcatttg aagaactgta tgaagcaggt aaaatcaaag caatcggtgt
    77821 gagcaatttc ttaccccacc atctagatac actagcaaaa acagcaaaaa ttatgccgat
    77881 ggtcaatcaa gttttccttg caccaggcga attacagaca actgtagttg agtatgcaaa
    77941 aaaacatgat atgatcctcg aagcatatag tccattaggc actggtaaaa tcttcgatgt
    78001 gcctgaaatg aaacaaatcg ctgaagcaca tgacaagacg attgcacaag ttgctttgcg
    78061 ttggtcgctg caacacgaat tccttcctct tcctaagtca gtgacaccaa gcagaatcaa
    78121 agaaaatact gaattatttg attttgaatt aacagaagaa gaaatgaaac aaatcgatca
    78181 gttagatggt gtagtcggca aagcaaaaga cccagatact acccaatttt aagtcgaaag
    78241 gtgattttat ggatccaatc gaacgcttga attctctctc ggaagaagtt acccagactt
    78301 ttcattccga ttttgtcttt ttgatcgatg cagagaaaat ccaacatttt cctgcaagaa
    78361 actggacaca tgatcaaatc atcgaagaac tgaaaaaacg gttcgatcat tcgctaatgg
    78421 tcaaaccctg gcacgaacat gaagtcattt attcacctga actacctgtt tttgccttga
    78481 taccgaaaaa atagaaagaa tggaacggat tatgtatata tacataatcc gttccatttt
    78541 ttatttttct ttcaaaaaac gggaaaatac gcaaattttt ctgttgtaat ttgcgtatac
    78601 gcaagttttt ttgcattact ccttttcatt tggctgtttt tttcttataa tcgaaagggt
    78661 atgagcgaag gagagaacat atgaagaaaa aacagaaaca aaaattatta aatcaattcc
    78721 gtccttcatt ggatggtgta agaggacaat tattccgttc tctagaagaa gagagcttgc
    78781 gacgttatgg tttgccgtta caagtcatgc ttgatcccaa aaaccgtcaa atcttaacga
    78841 tcggattagc tgggaaaaca aatgaagata cacggatcaa cgtgccaatc gatgacaact
    78901 tcaatactgt attaaagaga attcgttcag gagaaaacgg gattttcgaa cgattccgtg
    78961 acaatcttct tattgaaatc gtgtcttact ggaatgatca acgattgaaa aacgatgaac
    79021 caactgcgca agctgttgcg acaccagtta cagaagaaac agtcagtgtg ccagaagaaa
    79081 aaacggaaaa accaaaagcg gacacaactg aaaatacacg ctcaactgat acgaatgccg
    79141 ctttaagttt tgctgatttt tcaaaagccg ttgctgagta tcctaaattt tacacagaac
    79201 aaaatgaaca tgaagtagtt attaatgaaa aaaatgctga tgaacctcgt aaattagcaa
    79261 ctatttctat gactactgaa aatgagttcg ttatcgaaaa agcactcgaa cggaaataca
    79321 aagtcaaatt gactttgatt cctttgatcg aacaatttgc agcga
//
LOCUS       DAYYGS010000003        61712 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000003 DAYYGS010000000
VERSION     DAYYGS010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.003"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(132..509)
                     /locus_tag="ACHA44_000169"
     CDS             complement(132..509)
                     /locus_tag="ACHA44_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903497.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            765..1493
                     /gene="purC"
                     /locus_tag="ACHA44_000170"
     CDS             765..1493
                     /gene="purC"
                     /locus_tag="ACHA44_000170"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFM8903498.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1493..1747
                     /gene="purS"
                     /locus_tag="ACHA44_000171"
     CDS             1493..1747
                     /gene="purS"
                     /locus_tag="ACHA44_000171"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HFM8903499.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA44_000172"
     CDS             1749..2420
                     /gene="purQ"
                     /locus_tag="ACHA44_000172"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HFM8903500.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2421..4643
                     /gene="purL"
                     /locus_tag="ACHA44_000173"
     CDS             2421..4643
                     /gene="purL"
                     /locus_tag="ACHA44_000173"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HFM8903501.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4628..6067
                     /gene="purF"
                     /locus_tag="ACHA44_000174"
     CDS             4628..6067
                     /gene="purF"
                     /locus_tag="ACHA44_000174"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFM8903502.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6090..7142
                     /gene="purM"
                     /locus_tag="ACHA44_000175"
     CDS             6090..7142
                     /gene="purM"
                     /locus_tag="ACHA44_000175"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFM8903503.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7139..7717
                     /gene="purN"
                     /locus_tag="ACHA44_000176"
     CDS             7139..7717
                     /gene="purN"
                     /locus_tag="ACHA44_000176"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFM8903504.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7714..9249
                     /gene="purH"
                     /locus_tag="ACHA44_000177"
     CDS             7714..9249
                     /gene="purH"
                     /locus_tag="ACHA44_000177"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFM8903505.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9265..10539
                     /gene="purD"
                     /locus_tag="ACHA44_000178"
     CDS             9265..10539
                     /gene="purD"
                     /locus_tag="ACHA44_000178"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFM8903506.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10661..11575
                     /locus_tag="ACHA44_000179"
     CDS             10661..11575
                     /locus_tag="ACHA44_000179"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFM8903507.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11708..13273)
                     /locus_tag="ACHA44_000180"
     CDS             complement(11708..13273)
                     /locus_tag="ACHA44_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903508.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13266..14717)
                     /locus_tag="ACHA44_000181"
     CDS             complement(13266..14717)
                     /locus_tag="ACHA44_000181"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8903509.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14731..15747)
                     /locus_tag="ACHA44_000182"
     CDS             complement(14731..15747)
                     /locus_tag="ACHA44_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8903510.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA44_000183"
     CDS             15967..17055
                     /gene="chvE"
                     /locus_tag="ACHA44_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8903511.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA44_000184"
     CDS             17109..18635
                     /gene="mmsA"
                     /locus_tag="ACHA44_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8903512.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA44_000185"
     CDS             18637..19842
                     /gene="mmsB"
                     /locus_tag="ACHA44_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple monosaccharide ABC transporter
                     permease"
                     /protein_id="HFM8903513.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA44_000186"
     CDS             20099..21076
                     /gene="guaC"
                     /locus_tag="ACHA44_000186"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFM8903514.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21340..22764
                     /locus_tag="ACHA44_000187"
     CDS             21340..22764
                     /locus_tag="ACHA44_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8903515.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            complement(22806..23063)
                     /locus_tag="ACHA44_000188"
     CDS             complement(22806..23063)
                     /locus_tag="ACHA44_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903516.1"
                     /translation="MPIRILTALVNKITIRKAFYISSAFFLFSNQTYNHLFLFKNVGV
                     IQFCCKHLFYLHIVNGRTNNGFFLVSLYRILFPKKKKQLEI"
     gene            23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA44_000189"
     CDS             23225..24334
                     /gene="pdhA"
                     /locus_tag="ACHA44_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HFM8903517.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24337..25314
                     /locus_tag="ACHA44_000190"
     CDS             24337..25314
                     /locus_tag="ACHA44_000190"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HFM8903518.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25339..26982
                     /locus_tag="ACHA44_000191"
     CDS             25339..26982
                     /locus_tag="ACHA44_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFM8903519.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA44_000192"
     CDS             26990..28396
                     /gene="lpdA"
                     /locus_tag="ACHA44_000192"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFM8903520.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28558..28833
                     /locus_tag="ACHA44_000193"
     CDS             28558..28833
                     /locus_tag="ACHA44_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HFM8903521.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28839..29606
                     /locus_tag="ACHA44_000194"
     CDS             28839..29606
                     /locus_tag="ACHA44_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFM8903522.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29774..31603
                     /gene="typA"
                     /locus_tag="ACHA44_000195"
     CDS             29774..31603
                     /gene="typA"
                     /locus_tag="ACHA44_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFM8903523.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA44_000196"
     CDS             31864..33636
                     /gene="recQ"
                     /locus_tag="ACHA44_000196"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFM8903524.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33750..34052
                     /locus_tag="ACHA44_000197"
     CDS             33750..34052
                     /locus_tag="ACHA44_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HFM8903525.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34055..35218
                     /locus_tag="ACHA44_000198"
     CDS             34055..35218
                     /locus_tag="ACHA44_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8903526.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35244..38672
                     /locus_tag="ACHA44_000199"
     CDS             35244..38672
                     /locus_tag="ACHA44_000199"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HFM8903527.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38693..39817
                     /locus_tag="ACHA44_000200"
     CDS             38693..39817
                     /locus_tag="ACHA44_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP-associated domain-containing protein"
                     /protein_id="HFM8903528.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39798..40244
                     /locus_tag="ACHA44_000201"
     CDS             39798..40244
                     /locus_tag="ACHA44_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8903529.1"
                     /translation="MIAVIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESA
                     KVCQLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQ
                     LQSLLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40228..40566
                     /locus_tag="ACHA44_000202"
     CDS             40228..40566
                     /locus_tag="ACHA44_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HFM8903530.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA44_000203"
     CDS             40685..41245
                     /gene="rsmD"
                     /locus_tag="ACHA44_000203"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFM8903531.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA44_000204"
     CDS             41242..41733
                     /gene="coaD"
                     /locus_tag="ACHA44_000204"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFM8903532.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41723..42766
                     /locus_tag="ACHA44_000205"
     CDS             41723..42766
                     /locus_tag="ACHA44_000205"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HFM8903533.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42925..43653
                     /locus_tag="ACHA44_000206"
     CDS             42925..43653
                     /locus_tag="ACHA44_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFM8903534.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43769..44014
                     /locus_tag="ACHA44_000207"
     CDS             43769..44014
                     /locus_tag="ACHA44_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8903535.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44011..44229
                     /locus_tag="ACHA44_000208"
     CDS             44011..44229
                     /locus_tag="ACHA44_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFM8903536.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44365..44904
                     /locus_tag="ACHA44_000209"
     CDS             44365..44904
                     /locus_tag="ACHA44_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HFM8903537.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44897..47170
                     /locus_tag="ACHA44_000210"
     CDS             44897..47170
                     /locus_tag="ACHA44_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFM8903538.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47208..48254
                     /gene="holA"
                     /locus_tag="ACHA44_000211"
     CDS             47208..48254
                     /gene="holA"
                     /locus_tag="ACHA44_000211"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFM8903539.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA44_000212"
     CDS             complement(48343..48597)
                     /gene="rpsT"
                     /locus_tag="ACHA44_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFM8903540.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(48997..49266)
                     /locus_tag="ACHA44_000213"
     CDS             complement(48997..49266)
                     /locus_tag="ACHA44_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903541.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49528..49656)
                     /locus_tag="ACHA44_000214"
     CDS             complement(49528..49656)
                     /locus_tag="ACHA44_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903542.1"
                     /translation="MFEKLKYFIGTIGWFLLAVLLILGLAYLLFPDLIQNVFDTAF"
     gene            complement(49736..50191)
                     /locus_tag="ACHA44_000215"
     CDS             complement(49736..50191)
                     /locus_tag="ACHA44_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFM8903543.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50329..52257)
                     /locus_tag="ACHA44_000216"
     CDS             complement(50329..52257)
                     /locus_tag="ACHA44_000216"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8903544.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52454..53287)
                     /locus_tag="ACHA44_000217"
     CDS             complement(52454..53287)
                     /locus_tag="ACHA44_000217"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFM8903545.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53479..54183)
                     /locus_tag="ACHA44_000218"
     CDS             complement(53479..54183)
                     /locus_tag="ACHA44_000218"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8903546.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54206..54832)
                     /locus_tag="ACHA44_000219"
     CDS             complement(54206..54832)
                     /locus_tag="ACHA44_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8903547.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55158..57533
                     /locus_tag="ACHA44_000220"
     CDS             55158..57533
                     /locus_tag="ACHA44_000220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFM8903548.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57557..58048)
                     /locus_tag="ACHA44_000221"
     CDS             complement(57557..58048)
                     /locus_tag="ACHA44_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HFM8903549.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58235..59155
                     /locus_tag="ACHA44_000222"
     CDS             58235..59155
                     /locus_tag="ACHA44_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8903550.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59309..59653
                     /locus_tag="ACHA44_000223"
     CDS             59309..59653
                     /locus_tag="ACHA44_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HFM8903551.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59843..60343)
                     /locus_tag="ACHA44_000224"
     CDS             complement(59843..60343)
                     /locus_tag="ACHA44_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903552.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            60871..60954
                     /locus_tag="ACHA44_000225"
     tRNA            60871..60954
                     /locus_tag="ACHA44_000225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:60905..60907,aa:Leu,seq:cag)
     gene            complement(61278..>61712)
                     /locus_tag="ACHA44_000226"
     CDS             complement(61278..>61712)
                     /locus_tag="ACHA44_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8903553.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        20241 a        10663 c        13218 g        17590 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct taaatgtaca
      661 tatttttgtt gatttataaa aagatgattg ctaaaattta aagtgaaaaa acgaacaaaa
      721 aacgatgtat ttgcttttaa tgtaaataaa aaggagagac gtacatggag aagaaagaac
      781 ttttgtatga aggaaaagcc aaaaaaatat atcttacttc tgatgagaac ttgatttgga
      841 tagaatattt agatcaagca actgcgttga atggcgcgag gaaagaccag ataagtggaa
      901 aaggagaatt gaataatcaa atcacaagtg caattttccg ttatctgact caaagaggaa
      961 taaaaaatca ttttattgaa caattgtcta aaaccgaaca attagcaaaa aaagtacaaa
     1021 ttatccctct ggaagtggtt atccgaaata ttgcagcagg aagtttctca aaacgtctag
     1081 gtgttcccga aggtaaagaa ttcatcaaac caatcattga attctactat aaggatgacc
     1141 agttggatga tccatttatc aatgaagagc atatctattt cttacaactt gcaaagccgg
     1201 aagaaatcag tgagctgaaa gcccaagcgt tggctattaa tgaaattttg accgaattat
     1261 ttgctaaggt cggtttggtt ttagtagatt tcaagctaga atttggccgg tcgaatggac
     1321 aaattttgct tgcggatgaa gtaacaccgg atacttgccg attatgggat gaacgtacga
     1381 atgaacatat ggacaaagac gtttaccgca gagatctagg tgatttgatc ccagtctatc
     1441 aagaagtata taaccgtcta tcggaactaa ctgaagaaaa ggagcgagta tgatgtattt
     1501 cgtaaaagta tatgtaacct ataaagattc agtgttagat cctcaagggg aggcagtaaa
     1561 aaaagcagtc catcgtatgg gatatgaaac aatcgaagat atccgaatag gaaaatactt
     1621 tgagatacaa cttgctgatg cagaagaacc aatcgataca gtgattgaaa cgatttgtga
     1681 caagctgctt gcaaatgtaa atatggaaac ataccgatat gaaatcaaaa aaatggagga
     1741 agtataacat gaaatttgcg gtcatcgttt ttccaggttc aaattgtgac gcagatatgt
     1801 tatgggcagt tcgcgagatc atgggcgctg atgccgaatt tgtacggcat gatgagtcct
     1861 ctttagaagg atttgatggg gttttactgc cgggaggatt ctcctatggt gactatttga
     1921 gatgcggtgc gattgctcgg ttttctacaa tcatggaaga agtgattcgt tttgctgaag
     1981 aagggaaacc agtattcggt acatgcaatg gattccagat attgacagaa gcaggacttt
     2041 tgccaggtgt actgagacga aatgagtctt tgcattttat ttgcaaaact gtaccattga
     2101 aagtcaaccc caagacggcc tttacttcag agtatgaagc cgacgaaata atctatcttc
     2161 cagttgctca tggggaagga aattatttct gtgacgaaga aacacttcaa gaactaaaaa
     2221 aaaataatca gattgccttt acttataatg acaacatcaa cggcagcgtg gaaaacattg
     2281 cagggattac gaacaaagca gggaatgtgt taggaatgat gcctcatcca gaacgtgcag
     2341 tagaagactt gctaggatca gaagatggga aacggttctt tgcttccatt ttaaaaaact
     2401 tcggaaaggt gaccgtatag atgaaaacat tgaaagaacc gacagctgaa gagatcaaga
     2461 aaaaaagaat ctatgccgaa tggggattga ctgatgaaga atatcgaatg atcgaagaag
     2521 atatcttagg aagaatgcct aattatacgg aaactggact gttttctgtt atgtggagcg
     2581 aacattgttc ctataaaaat tctaaaccgg ttctacgcaa atttcctact agtggacctc
     2641 atgtgttgca aggccctgga gaaggcgcag gcatcgtcga catcggtgat ggactagcag
     2701 ttgtcttcaa agcagaaagt cacaatcatc cttcagccgt ggagccttat gaaggagctg
     2761 cgacaggagt tggcggaatc atcagagata ttttcagcat gggggctcgt ccaattgcgt
     2821 tgctagattc attgaggttc ggcgaattag ataatccaag aacgaaatat ttactagaag
     2881 aagtagttgc aggtatcggc ggatacggca attgtatcgg cataccgact gtaggcggcg
     2941 aaattgcatt tgatccttgt tatgaaggaa acccattagt caatgcaatg tgcgtaggtt
     3001 tgattgaaca caaacatatc cagaaagggc aagccgctgg cgtcggtaat tcgatcatgt
     3061 atgtaggtgc aaaaacaggt cgcgacggta tccacggagc aacttttgcc tcagaagaat
     3121 ttaatcaaga agaagaacag caacgttctg ctgtacaagt aggcgatcct tttatggaaa
     3181 agctgctgct tgaagcgtgt ttggaattaa tcttgcatca ttcggattct ctaataggta
     3241 tacaggatat gggagcagca ggacttgttt catctagtgc ggaaatggct tcaaaagctg
     3301 gctcaggact tatattgaca ttagatgaag tcccacaaag agaaactggg atgacacctt
     3361 atgaaatgat gttatcggaa tcacaagaac ggatgttgat ttgtgttaaa tcaggagagg
     3421 aagaaaaaat ccaagaacta tttcagaagt atgatctaga tgcagtcact atcggtaaag
     3481 taacagacga cgggcaatat cggctatatc atcaaggaga agaagtagca aatctgccag
     3541 tcgatgcatt agctgaagat gcaccagttt atcataaaga aatgaaagaa ccagctcgaa
     3601 tcgtagaatt tcagcagtta gctccttatc agccagttat cgaagaacct ggacaagtat
     3661 tgcttgatct gctgcagcaa ccgacgattg cttcaaaacg ttcaatctac gaaacttatg
     3721 attcacaagt acaaaccaat acagttgtcc gtccaggaag cgatgctgct gtgatgaggg
     3781 ttagagggac gaataaagcg ttagctatga ccacagactg caacgcgcgc tatctgtatt
     3841 tggatccaaa aatcggagga caaatagcag tggcagaagc tgctagaaat attattgcta
     3901 gcggtggtaa gcccttggcg atcaccgact gcctgaacta tggttctcct gataaaccag
     3961 aagttttttg ggaattatcg acttcagctg acggaattgc tgctgcgtgt gaaacgttag
     4021 gaactcctgt tatttcagga aatgtctctt tatataatga aacggatggt caagcaatct
     4081 atccaacacc aatgattggt atggtcggat tgattgagga tcataaacat atcactactc
     4141 aggaattcaa aaaatcaggg gatctaatct atatattagg taagacgttt gcagattttg
     4201 atggcagtga attacaaaag atgcagcttg gaagaatcga aggtgtgatc cgaaactttg
     4261 atctatccat tgaaaagaga aaccaagagt tagtattgac agccatccaa aacggattga
     4321 ttgaaagtgc tcatgactgt tcagaaggtg gcttggcaat tgcactggca gaatcagctt
     4381 tcaaacatca gctagggata tctgtacagt ttgaattgtc ctctgctcaa ttatttgcag
     4441 aaactcaatc aagatttgtg ttgacagtcg caccggaaaa taagacacga ttcgaagaaa
     4501 tgatgggaga tgctgctgtt ttagcaggta aggtgacgga tgaagcgata atcgaaattt
     4561 cagcaactga cggtcagatc aaaatagaaa ctgcagtagc aagaaagtgc tgggaggatg
     4621 caatcgtatg tcttatgaag tgaagagttt aaatgaagaa tgcgggatct tcggtgtttg
     4681 gggtcatcca gacgccgcta gtgtggcata cttcgggctc catagtcttc agcatcgagg
     4741 acaagaaggt gcaggtatcg tgtccaatga tcgaggaaag cttaaggggc atcgagattt
     4801 ggggctgctt tctgaggtat tcaaagatca aagaaaattg cagcagttaa caggagaagc
     4861 tgcaatcgga catgtacgtt atgcaacagc aggcaatgga agtgtggaca atattcagcc
     4921 ttttctattc aaatttcatg atcagtcgat cggcttagcg cataatggta acttgatcaa
     4981 tgctcgttcc cttagaaaag agttagagga aaatggggcg atcttccatt ctaactccga
     5041 tacggagata ttgatgcatt tgatccgacg aagtgaagaa acagattttt taaataaatt
     5101 aaaaagtgct ttgaatgaag tcaaaggtgg ctttgcctat ttattaatga cagaaacggc
     5161 aatgattgcc gctttggacc caaatggttt tcgtccatta gcaatcggac aaatgaaaaa
     5221 cggagcatac gtgattgcct cggaaacttg tgcattagaa gttattgggg cacgttttat
     5281 aagagatgtc cagcctggtg agatagtgat catcaatgac gaaggaatcc agattgattg
     5341 ctttacgaag gacacgcagc tttctatttg ttcgatggaa tatatctatt ttgccagacc
     5401 agattcaaac atcgcaggga tcaatgtgca tactgcacgg aaaaatatgg gacggaattt
     5461 agcgaaagaa tcgccagtaa aagcagatat ggtcgttggc gtacctaatt cttctttgtc
     5521 agccgcaagc ggttatgcgg aagctagtgg aatcccttat gaaatcggat tagtaaaaaa
     5581 tcaatacatt gcccgaacat ttatccagcc aacgcctgag ctaagagagc aaggtgttcg
     5641 aatgaaatta tctgctgtac gaggagtagt ggaaggtaaa aaggtgatta tggtcgatga
     5701 ttcaattgtt cggggtacga caagcagacg gatcgtccat cttttgaaag aagcaggggc
     5761 aaaagaagtc catgtgagga ttgcttctcc tcctttgaaa tatccatgtt tttacgggat
     5821 tgatattcaa acgcgtaaag aactcattgc ggcaaatcac acgattgatg aaatcaaaga
     5881 atgtatcggt gcagattctt taggtttttt aagtgaagag ggtctgatcg aatcgatcgg
     5941 tctggaaaaa gatgctccgt attcaggatt gtgtatggct tatttcaacg gagattatcc
     6001 gacgccgtta tatgactatg aagaaaaata tcaggaatca ctaaaagaaa aagtatcgtt
     6061 cttttaaaag cagaccaaga agggagtata tgaattcaat ggcaaatgca tacgcaaaag
     6121 ctggcgtaga tgtcgaagct ggatatgaag tagtagaacg gatcaagaag catgtcaaaa
     6181 aaacggaacg tttaggtatg atgggcgcta taggaggatt cggcggttgt tttgatttat
     6241 cggtctttca attaaaagag ccagtccttg tctcggggac cgatggcgtg gggacgaaat
     6301 tagcagtggc aatcgaagca gacaaacacg atacaatcgg gatcgactgc gtagcgatgt
     6361 gtgtcaatga tatcttagca caaggcgcag agccgttata tttcttagac tacttagcaa
     6421 cggggaaaaa tgatccgtta cgtttggaaa atgtagtaaa aggtgtagca gatggatgtg
     6481 ttcaaggaaa tatggcgctg attggcggag aaacagctga aatgcctggt atgtactcag
     6541 gtaatgatta tgatctagcc ggatttgctg tagggatagc tgaaaaatct caattattga
     6601 caggagaaac gatcaaccca ggagatgtgt tgcttggaat agcatctagc gggattcatt
     6661 caaatgggta ctctcttgtt cgcaaaattt tctttgagca gcagcagttt tctttatcat
     6721 caaaactgcc agaattgaaa aaaacacttg gcgaagaatt gcttgagcca acgaaaatct
     6781 atgtttcttc cttgcttccg ctgatcaaag aaaagaaaat aaaaggagct gcacatatca
     6841 ctggtggagg gtttgtagaa aatatcccgc gaatgttacc agaatctatg gctgcacaaa
     6901 tcaaacttgg ctcatggccg attcttccaa tattcggctg tttacaaaaa tatggtgaaa
     6961 ttcctgagat ggagatgtat gaaatcttca atatgggcat cggtatggtc ctggcagtcc
     7021 aagaatcgaa agtaaaagaa atagtacacg aaattgagca agctggcgaa aaagtttatg
     7081 aaatcgggaa agtgatcgcc aaagaaaaac atcgtgtggt gtttatagga gaagaacgat
     7141 gagaatcgct gtttttgctt ctggaaacgg aagcaacttt caagcgctag ccgattatct
     7201 atcgaaaaaa ggattggaat cttcgattga ctggttgttt tgtgatcagc ctgaagcata
     7261 tgttttgaaa cgtgcaactg cgttgagtgt accggcagat tgtttttcac ctaaagaatt
     7321 tgattcaaaa aaagagtatg aagaagcaat tttgcataaa ttgaaagaaa aaaagatcga
     7381 tttgatcgta ttagctggat atatgcgaat cattggaccg gtattgctga aaaattatga
     7441 taaaagaatc atcaatatcc atccgtctct tttacctgcc ttccctggtt tgcatggcat
     7501 acgtgatgcc tttgaagctg gagtaaaaga aacgggtgtc acaattcatt atatcgacca
     7561 aggtgtagat actggaccaa tcatccggca ggaaaaagtg agaattgaac aggaggacac
     7621 gttcgattct ttagaagaaa aaatccatag agtagagcat cgaatctatc cagaagtgat
     7681 tagtgaaatt attgaaaatg gaggctatct gaaatgacaa gagcattgat cagtgtatca
     7741 gataaaactg ggattgtagc attcgcccaa ggtttgattg aacaaggagt agaaatcatt
     7801 tcaacaggag gaacaaagca tgcgttggat aaagctggga tcaaaacgct ttcgatcgaa
     7861 gaagtgacag ggtttcctga aatgatggat ggccgtgtca aaacacttca tcctaaaatc
     7921 catggtggtc ttttaggaag acgcgatctc cctagccata tggaggcaat ggaaacggaa
     7981 aatatccaac caatcgactt tgtttgtgtc aatttatatc ctttcaaaga aacgattatg
     8041 aaaccagatg tcgaaatagc agaagctatt gaaaacattg atatcggtgg tcctagtatg
     8101 ctccgtagtg cagcaaaaaa ccatgaattt gtcacctctg ttgttgatcc atctgactat
     8161 gaagaagtac tttcagaatt acgtcagtat gggcatactt cactttcttt cagacaaaaa
     8221 ctagcggcaa aagtctttcg tcatactgcg gcatatgatg cactgatcgc tcaatactta
     8281 acggattggg tggaagaaaa agaaccggaa aaattgacac ttacgtatga acgaaaacaa
     8341 acgcttcgtt acggtgaaaa tagccatcaa gccgctgcct tttatcagtc cgctcttctg
     8401 gaaaaatatt cgatcgcagc agcaaaacaa ctccatggga aagaactttc ttataataat
     8461 atccgagatg cagatgctgc gctacggatc atgaaggaat atgacgagcc aacggtagtc
     8521 gccttgaaac acatgaatcc ttgtggtatt gggacagcat ctacgatttt agaagcgtgg
     8581 gaagcagcgt atgaagcaga tcctgtttca atctttggtg ggatcatcgt tttgaatcga
     8641 gaagtagatc tgccaacagc agaacagatg cataagctgt ttctagaaat catcattgcc
     8701 ccttcttata cacaagaagc tttggaatta ctgcaaaaaa agaaaaacat ccgattactg
     8761 acagtagact ttgcgcagga agaaaatcaa aatcgagaag aaacggtctc agtcttaggt
     8821 ggacttcttg tacaagatca agatctggca atagaaaatg aagaagagtg gaaagtcgtg
     8881 acaaaacgta agccgactcc agcagaagca aaagcattag cgtttgcttg gagagctgtc
     8941 aaacatgtga aatctaatgc gatcgtactg gcaaacgaac gtcagacagt aggaatcgga
     9001 gccgggcaga tgaatcgagt cggttcagta aaaattgcga ttgaacaagc gcaagagaaa
     9061 atggaaggtg cagttttagc tagcgatgct tattttccaa tggatgattc ggttgaatat
     9121 gcagccaaac atggtataaa agcaatcatt cagccaggag gaagcatcaa agaccaagat
     9181 tctattgata tggcgaataa atacgatcta gcaatggtct ttactgatgt tcggcatttt
     9241 agacattaga gaggagtcgg ataaatgaac attttggtga tcggtgcagg tggtcgtgaa
     9301 catgcaataa gtaaaaaact tttagccagt ccgaaggtgg atacggtgta ttgtgcgcca
     9361 ggaaatccgg ggatgacaaa agatggtatc aattgtgtcg ctatcagtga gcgagatcat
     9421 tcagcgttaa tccattttgt taagaaagag gctattgatt ggagtatcgt tggtcctgaa
     9481 gttcctttgt taaatgggat cgtcgatgat tttatggcgg aaggattgaa aatcttcggt
     9541 ccgacaaaag cagcagctct gatcgaaggt tcgaaaagtt ttgctaaaca attaatggat
     9601 acttatcaaa tcccgacagc taaatccaga agttttgaat gttacgaaaa agcagcagcg
     9661 tatatcaaag aaaatggtgc gccaatcgta gtgaaagcgg acggcttagc agctgggaaa
     9721 ggtgtagtcg tggctaccac agtgaaggaa gcgctggatg cggcagaaca aatgttgaac
     9781 cagcatcgat ttggggatag tagcaaaaaa atcgtcgtag aagagttttt ggcaggagaa
     9841 gaattctccc ttttggcgtt tgtccgtaat accaaggtgt atccgatggt catttcgcaa
     9901 gatcataagc gggcatttga taacgataaa gggccaaata caggcggaat gggtgcttat
     9961 tcaccagtac cgcaaatttc aaaaaagatg attgaaaaag ctgtggaaga agttttaaaa
    10021 ccagcggctt tgggaatgaa agaactagga cgctctttca cgggtgtttt atatgcgggt
    10081 ttgattgcta cgaaggaagg accaaaagtg atcgaattca atgcgcgttt cggtgatcct
    10141 gaaacgcaag tcgttctttc tcggttgaag agtgactttg cgcaagtaat cgatgattta
    10201 ttagagaatc gtacagtaga gttaaagtgg cagcaagaag gatacaatgt aggcgttgtg
    10261 gttgcagctg ccggttaccc cgaagaatat gagacaggaa tggttctacc agattttctg
    10321 gaagaagagc tttctgttta ctatgctgga gtttcctcaa ataacggcaa actagttgga
    10381 gcaggaggac gtctttattt agtagaagca tacggtgaga cgatcaaaga agcacagaaa
    10441 aaaatttaca ccgcgttaat ggataaaacg acagatggaa cattttatcg tacagatatt
    10501 ggagaaaaag ctgccaatag cgaactgatc gtgaagtaaa tcagctgctt ccatttataa
    10561 aaaatacaac aaacataatc ggaagcgccg attttagtga agccttttca taaagagtgt
    10621 ggtacacttt tgttgtgtat ataaataagg aggagttcag atgaaaaaaa ttcaattagg
    10681 tacttctgat cagaaagcag ctgcagtgat cttaggctgt atgcgtatca acaatgcgga
    10741 agatccagta aaagtgattg agacagctta tgaaaacggc attgattttt ttgaccatgc
    10801 agatatttat ggaaacggtg aatgtgaaga aatctttgca gatgctctag caaaaacatc
    10861 aatcaaacga gaagatcttt ttattcaaac aaaatgtgga atcgttccag gtgtgatgtt
    10921 tgatttttct aaaaaacata tcattgaatc tgtagaaaaa agtttgaccc gtctgaaaat
    10981 ggattatgtg gatgctttac ttcttcatcg tccagataca ttggtagaac cagaggaagt
    11041 agctgaagcg tttgatgaat tggaacggtc aggaaaagtt cgttattttg gcgtcagcaa
    11101 tcaaaaacca atgcaaatcg aattgctgaa aaaatatgtt cgtcaaccat tagtagctaa
    11161 tcagctgcaa tttggcttga aacatactgg aatgatcgat caagggattc atgtcaatat
    11221 gacagatgaa gcaagtatcg atcatgacgg tagtgtactt gattattccc gattgcatga
    11281 tatgacgatc caagcatggt caccatacca gtatggattt tttgaaggtg tatttatcgg
    11341 gaacgaaaaa ttccctgaat taaaccaaac attagaagaa ctagcagcta aatatcaaac
    11401 gacaccaact ggcttggctt ctgcttggat tttacgccat ccagccaata tgcaagtgat
    11461 cgccggtacg atgaacgtag ggcgaatcaa ggaaatcgct caagcttcag aaattaatat
    11521 gtctcgggaa gattggtacg aattgtaccg tgctgctgga aacattttac cttaaagatt
    11581 gtcataagca ggtcaaaagg gctataaatt aaaaagacag aataacttga aagctgtttt
    11641 cagcttcgag atattctgtc tttttttatt gcaaatggaa agagtatcat tgtacggatt
    11701 tcgtttatta gttatttttg tttttaagga aaagctgtgt ccgatattct tttggagaaa
    11761 tcccttgttt tttcttaaat aaataactga aatagttagg gtcattataa ccaatttcaa
    11821 aagcaatatc tgaaatacga tagtctgatt gctgcaataa tttttttgct aattcgattc
    11881 tttgttctgt cagataatcg atgaatgttt ggttaaacga ttgagaaaaa atcgtactga
    11941 aatgagaggg acttaatgca acttgttgag cgacccaagt taaggacata tctggatcgg
    12001 taaaattttc tttgataaaa tccaatgcat ggttcaaaat tggctgatat ttgttccttg
    12061 aaggctgtga gggcagagca gttaagaaag aaatgaacat ataaactgtc gaacggtaaa
    12121 gcgagtcgtc ggcgctgatt gctaagcgtt gactggtgga tgtgcaatga accatttctt
    12181 gcgggatttt cttttctttc aggcagacat gctggagtag cttagtcaag acaaataaac
    12241 gatataatcg tgttcgagtt ggtgtgcctt gtggtttcgt caagtgttcg atgtacttag
    12301 atacttcgtt tttcgataat gaagacaaat caagtgagag gtcaaagtaa aagacagaat
    12361 caactggaag tttcgcttct tgaatcaaat cctcatagct gataatttgt tctttaaggg
    12421 aactggcggt ttcaagtatc atctgcgtga tctcaaaaga tgcaggtaat tcgctgattc
    12481 tttctgcgac aggaccaatg gcgacagaaa aattattttt ttgtcccttt tctaattcat
    12541 ggaccaacgt ttgggctacc tgataactgg tctcgatcac attttcttca agagaatcaa
    12601 ctattaaaaa tttgatgaat cgagaggaaa cagcagagaa gatcacttgt tgattctccc
    12661 taaataaaaa cttcaaataa tcactgagat gagcgtaatc ttcaaagtct gtactgactt
    12721 ggtttgctgc taataatacg gtcatttttt tccctgctaa agagcgattg aaagattcag
    12781 caagtttcaa tgtttcactg atcgtaagtg agcctttgaa caaaccgttc aagaaatgat
    12841 tttttttcaa ttcgaaaaca agatcagaag aagtgtgcgt ttctttttgc tgcaaatcta
    12901 aagcagttac tgctttttct attgcttgtt tcaattcgct acttttaatc ggtttaagca
    12961 gataatcatc tgcctgtacc tggattgctc ctttcatata atcgaaatcg tcgaatccac
    13021 taataaatat gagacgagtc caaggaagta gtttccttgc ttcttttgaa aaagtcaacc
    13081 cgtccatgaa aggcatttgg atatcagtca gaatgatgtc gggctgtaag tcgataattg
    13141 aagacagtgc catctcacca tctccagctt cgccgacata agtgattgga taataagaac
    13201 caaggacagt tatttgttcc cgcaagttat tgcgaatgag gtattcgtct tcgatgataa
    13261 aaactttata catgtttgtt tcctccaatt gttttgggag atagagggat catcagtgtg
    13321 acagttgttc cttctcggta tttgctgtca atcgtgatag gtttttgatc atttccgtaa
    13381 taaagaagga gacgcttgtt tacgttgtaa agtccgtagc cgatactgaa atctgaatcg
    13441 atccctttgg ctaactcttt acgtatttct gccagttttt ctgcagggat gccaatcccg
    13501 ttgtctataa ctttgaattg gatcatctca tcaattttgc atgtggtaat acgaataaat
    13561 cctttgtgac ggacgtactt cgttccatgg tatacagcat tttcaatcaa tggctgcaag
    13621 atcattttta aaatagaaac aggaaaaagt gtttctggta tatcgatctt atagtcaagc
    13681 atatggccgt atcttatttt taagatgact agataatcat tgatatggct aatttctttt
    13741 tcaagaggaa tccagtcttg tcctttgctt aaagacgtac gaaaaaagcc ggaaagtgca
    13801 tgggtcatag tcagagcttc ttcaaagcag tcttgttcga tcaaagaaat aatggcatcc
    13861 aaactgttat aaataaaatg gggagtaatc tgagcttgca atacacgaac ttcactttgt
    13921 gctaaatggt attgtttcaa agcgttctcc cgtaaagact ggtttagatc ttccgccatt
    13981 ttattcatgc tagcggtaag tgtttgtaat tccagtgttt ccggcagggt taaacgaaag
    14041 ccaaggttcc ctgaagaaag ttgttctgac atctcgatca gctgattgat cggttcttgg
    14101 acctttctct ctaaaaagcg acgattcttt ttcgcataga aaaaaatggt actaagaact
    14161 ccaagttcca ctattgttaa tagaatgagc gagcggacaa gtgtcgtatt ttttttgctt
    14221 gctaagtcga tctccacacg aacaaaatct tgtaagatat ctgacagcag ttgagtgaca
    14281 gaatctacct gattgatgat ctcttcattg gtatccataa aatcttttgc caagatatta
    14341 ttgatcagct cgttttgata atcttccagt gtatcgatcg tacgtagcga tacatctaac
    14401 gtactaattt ctttaagtgt atttgtatta tttttgatct ctcgaatatc attcctaagt
    14461 tcttctagga tattttcctc attgaactca tctacggtta cttggccaga aatcaagtcc
    14521 cacatacttt ccaaaacttt ttgatcaacc ttcaaagcaa ttgcatctgc tttttggata
    14581 tttgcgatcg tctgttgata aatcaaaagc tgttgcgaat ataacagtat gctcagtagc
    14641 aaagggataa cagcaaatag aagcatggct ttgtttgctt gggcaaaaat ctgttttatg
    14701 cttaaatttt ttgtcatgat acttctccag tcaataattt ctggttggga tgttagctat
    14761 attctgctgg gaaaagacag tctcggaaat aaaaatatct ttaggtaatg tttttcctgt
    14821 ttgaatattt ccaaaataac gagtaacggt attggctaca taccagccga catcaggttt
    14881 acactcgact acacaattga cgattcctga gcgtagcttc tcgatcatat cttcttgtcc
    14941 atcaatagtt acaatgatca aatcttctcc aggtttgatt gatgtttctt ttatcgcagc
    15001 taaagcaccg tgagtcattt catcactatg gctgtataat acatcgattt cggataaatc
    15061 atttgtttct aaatatcgtt tcatctggtc ttttcctttc atccgaataa aatcaccgac
    15121 aattcggtga acaatagtca tcggaggttt atggtcgatg gttccatctt tttttagggt
    15181 tggtttacga ttgattgatt ccaaaaatcc ttcggagcgt agcttggtcg gtgtggagtt
    15241 ctctgaacca gcaagttcta ggattctgat ttcctgtttc tttgaatttg tgaagtgatt
    15301 gctgacaaat aaacccgccc gatttccttc tgctttgaaa ctggggccaa tgtgggtgac
    15361 gaaaagctcg ggatcattcg tttgaatatg tctgtcgaca agtatgacag ggatccctgc
    15421 tcgctttgct tcttttaata cagagtccca cccggattct tgcaaaggag taaaaatgat
    15481 catatccact ttataggcaa tgaacgtccg tatatcttgg atttgccgtt cctgattcat
    15541 gtagccattt cggtagagaa cttggtaatc ttctttttca agcgcagtga taattgattc
    15601 agtatgcttt ttccgccagc tactctccgt tcctgattgc gaaaaaccaa cgactagtgg
    15661 gtcaggtgct tcctttgtat taggtgtaca ccccatcatt tggtaagcga ttacaacaag
    15721 ggctaaaaaa aataaaacac gtttcacttt aaatcactcc atctggattt gttagtacta
    15781 tcttatcaga taaaagggaa aatcaaagtc aaaaggaatc aatcgaaaaa aatccaagaa
    15841 acttaaaaaa atccaagaca aaaaaagtac acctaaaaaa tttaaagtaa atcccataaa
    15901 aaaatcaatg ttaacgcttt ctttattagc tatactcggt gtatcaattg aaatggggga
    15961 taaaaaatga aaaacaaatt tagaaagatt gttgggacta tcgctatttt gaccgcaagc
    16021 attctattaa gtgcgtgtgg caattcagga tctgcagatg atagtacggg gtatgtagga
    16081 atcgctatgc cgaccaaatc agcagaaaga tggatcgctg atgggaacaa catggtctcg
    16141 gaattagaaa aactgggtta taaaacagat cttcaatatg gagaagataa agtagaaaat
    16201 caagtagcac aaatagaaaa tatgattaca aaaggggtag atacacttgt gattgcttct
    16261 atcgatggtt cggctttaac tgatgtgtta gctaaagcaa aagaagcaga tatcaaggtg
    16321 atcgcctatg atcgtttact gatgaattca gaaaatgtcg attattacgc gacttttgat
    16381 aattttggag taggtgtatc gcaagcagct tatattgaag agcacttagg attgaaagaa
    16441 ggaaaaggac cattcacgat tgaattattt ggtggctcgc ctgacgacaa caacgcacta
    16501 atcaattaca acggagtgat gtctgtttta caaccatata tggacaacgg tcagttggtc
    16561 gtgccatctg gacagacgag tttcagtcaa atcgctacgc ttcgctggga tggttctacg
    16621 gcgcaagcaa ggatggataa tctgttaagc gcaaattata ctgaccaaac attggatgct
    16681 gttttgtcgc catatgatcc aataagttta ggaatcattt cgtcattaaa aggcgtgggg
    16741 tatgggagtg aaagtaaacc tttacctgtg attactggac aagatgcgac agttgctggc
    16801 gttaaatcga tcattgccgg ggaacaaaca caaacgatct ttaaagacac acgtatctta
    16861 gcaaaaaata cgattgaaat gatcaaagca atcagtgatg gagaagaggt tccggtcaat
    16921 gatactgaaa cttatgataa tggggtgaaa actgtcccta cgtatttagc aaatacagta
    16981 tctgtcgata aagataatta tcaagcagag ctgatcgaca ccgattatta taaagaatcc
    17041 gatctgaaaa attaagagga accaaacaag gaactaaaga gatctaagtc agagagagga
    17101 taagaattat ggcggattgt attttaaaaa tggcagatat caccaaaaaa ttttctggaa
    17161 tccgagcgtt agattgcgtg aatctgatga ttgaacgtgg ggaaatccat gcattgtgcg
    17221 gagaaaacgg agcgggaaaa tcaacattga tgaatgtctt gagcggcttg tatccatatg
    17281 gctcctatga cggggaaatc ttttatgatg gaaagctgtg tcaatttaaa gatttgaaag
    17341 atagtgaaga aaaaggcatc gtcatcattc atcaagaatt agcactaagt ccttatcttt
    17401 caataaaaga gaatattttt ctcggaaatg aacaagcaga aagaggcgtg attgattggg
    17461 aactgacaga aaagaaaaca tatgaactgc tgaataaagt cggtttgtct atcgacccga
    17521 atcttctagt atcccagatt ggcgtagggc aacagcaact agttgaaata gctaaagcat
    17581 tatcaaaatc cgtccgtctg ttgattttgg atgagcctac cgctgcattg aatgaagaag
    17641 aaagcgcgaa tttgctgaga ttgatccgag agttgaaaga gcaaggtgta acctccatca
    17701 ttatttcaca taagctgaat gaaatcgtgg atatcgcaga tcgaatcacc attttacggg
    17761 atggacagac gattgagaca ctagaaaatg aaatgatcag cgaagagcat attattcgcg
    17821 gaatggtggg cagagattta gttgatcgtt atcctgaaag aaaaccggat attggagaag
    17881 tttattttaa agtagaagac tggacggtct atcaccctct tgatcacgag cgaatcataa
    17941 acgaccacat caacttgcat ttgaaaaaag gagagattgt aggaatagct ggactgatgg
    18001 gagcgggcag aactgaattc gctatgagtc tatttggaca ttcttatgga agcaagatat
    18061 ccggacgtat attaaaggaa ggggcagaga tatcagttaa gaccgttcct tcagccattg
    18121 caaacggact cgcttatgtc tcggaggacc gaaaatcttt aggactgaat ctgctgatgg
    18181 atattcggga aaacgtgacg ctgagcagtt tggataaaat aagtcaaaaa ggagtattag
    18241 ataaagaaaa agaagtaact gaggcagaaa atttccggaa aaagatgcgt ataaaaacaa
    18301 actccatcca tcaaattgtc agtagtctta gtggtgggaa tcaacaaaaa gtcgttttat
    18361 ccaagtggct gatgacacaa cccgatattc tttttttgga tgagccaacc cgtgggatag
    18421 atgttggggc aaaatatgag atctacacga ttatcgaaga aatggcagca aatggcaaag
    18481 ctgtctgtct tatctcttct gaacttcctg aaatattagg gatgtgtgat cgaatctata
    18541 cgatgaacga aggacgtatt acaggagaag tctcgcgaga agatgcagat caggaagtct
    18601 tgatgaaatt aatgacgaaa gaagaggtgg gttaagttgg aacaggcaaa aaatcttacg
    18661 gataaaaaag agaaaaaact aaatttgaaa agtagagcag ctgatctttt tggtcgctac
    18721 agcatggtga tcattttagc gggactactt attgcgtttc aattgatgac ggatggtatt
    18781 ttctggcgtc cattgaatat aactaacctt gttctgcaaa atagccatat attagtacta
    18841 gctgcaggga tgttattagt tgtgctctta ggtcatgtgg atttgtcggt agggtcagtg
    18901 atggcgtttg ttggtgcaat tgcgggcatg atgatggtca atcgccaaat gaacccgtgg
    18961 atgacagtta tcgcatgtct tgcagtaggc gggctgatcg gcgcatggca aggtttttgg
    19021 gtagcttatg cagggatacc ggcgtttatc gtgactttag caggattgct gatgtttcgt
    19081 ggactgacac aagttgttct aggaggccaa tctctagcac cttttccagc tgttttccaa
    19141 aaaatgtcta cgggttattt accagactta agcgaaggaa gcagccttca cttcttgaca
    19201 ttgattttag gaatgatcat cacgatcgtg ttagttgttg gtcaatggcg aacaagaaat
    19261 cgtcggaaaa atcatttatt tgaagtggaa tctccaacag ctttcttggt aaaggcggta
    19321 tttacagcag tcgttgtact aggaataact tatatctttg cctcttatca agggtatccg
    19381 attattttga tcattcttgg tttgatcgtt gcagcatatg ggtttttgac aaataaaaca
    19441 gtagcaggtc gacagattta tgcgacgggc ggaaatcgaa aagcggctga actatcaggg
    19501 atcaaaacaa agcgaatcac gttctgggta ttcgtcaaca tgggagtcat ggcagcttta
    19561 gctggattag ttttagctgc tcgattgaat gcggcaacac ctcaagcagg tacatcgatg
    19621 gaactggatg caatggcggc agtatatttt ggtggtgctt cgacttccgg cggcattgga
    19681 acaatcatgg gtgcgattgt cggtggcttg gtcatgggtg tattgaacaa tggcatgtcg
    19741 attttaggcg tcggggtaga ctggcagcag gcaatcaaag gattgatctt gcttttagcc
    19801 gttgtgctag acttgtataa taagaaaaaa cgaacaacgt aggagaacgc cggttattca
    19861 gaaaaaagac tgtgacaaga catttgtcac agtctttttt tcgctaaaag cgaacattca
    19921 ttgtttcgtt tgtatagact aaaaataata tatgtttttt taccattcgt ataaattctc
    19981 tttttgctct tgattttttt gcttaattga gttaaattac atagggcgct aaaaaaagcc
    20041 ttgattaaat taataatgtt cgtgttttgc tggatgtgaa catcaggagg ataatgtaat
    20101 gaaagtattt gattacgaag atattcaatt aattcctaat aaatgtatcg taaatagccg
    20161 ttctgaatgt gatacaacgg tcacattagg taaacataca ttcaaaatgc cagtagtgcc
    20221 tgccaacatg cagacgatta ttgatgaaac aatcgcagaa ttcttagcgg aaaatggtta
    20281 tttctatatc atgcatcggt ttgatgaagc agctcgaatt ccgtttatca aaaaaatgaa
    20341 aaagcgtgga ttgattacct cgatcagtgt tggcgtcaag aaagaggaat attcctttat
    20401 agagaagttg gcagaagaat cgctaaatcc tgattatatc actatcgaca tcgcgcatgg
    20461 acatgctaac tcagttattg atatgattca gcatattaaa aaatatttgc ctgaaacttt
    20521 tgtgattgcc ggtaatgttg gtacaccgga agccgtccgt gaattggaaa atgcgggcgc
    20581 agatgcaaca aaagtcggga ttggtccagg aaaagtatgt attacaaaaa tcaagacagg
    20641 ctttgggact ggaggctggc aactggcagc tcttagatgg tgtgcgaaag cagctcgcaa
    20701 gccgatcatc gctgacggtg ggatcagaac acatggcgat attgcaaaat ctgtccgttt
    20761 tggtgcgaca atggtcatga ttggttcatt gtttgccggt cacgaggaat ctcctggcga
    20821 aacaaaagtc gaaaatggaa tcgtgtataa agaatacttt ggttctgctt cagaattcca
    20881 aaaaggcgaa aaacgaaatg ttgaaggaaa gaaaatctgg atccaacaca aaggatcttt
    20941 gaaagataca ctagtggaaa tgcaacaaga ccttcaatca tctatttctt atgctggagg
    21001 aagagatctg gaagccattc gaaaagttga ctatgtcatc gtgaaaaatt cgatttttaa
    21061 tggagacaca atctaaaaaa taaataaaat attaggatgt tgtgaaaaag ttgtcctgca
    21121 ttaagtaata agaacagcca aacaaaaata gttctttata tgttttgttt ggctggaatt
    21181 attatcgcag atacaagctt ttgaacatca ttattcagaa taagggactg tgataagatt
    21241 tttgtcacag tcccttatgt tttgcaaaaa aagtttttct tttttctcgt aaattcggta
    21301 aaatcaaaag aaaaaataga caatagaaaa gagaagctta tgtgggaaaa agtagagaaa
    21361 aaacaaggac cgatttatcg tcaaattatg gaacagatta tgagagaaat cgaaaacgga
    21421 aggatcaatc ctggaagtca acttgaatca gaacgaaaac tagggcaact ttttggtgtg
    21481 aatcgttcca ccgttgtcca tgcattggaa gagcttaggg agcttggggt actaacgagc
    21541 aaacgtggca gcggatggtt tgttaatcag actgaatggg ggaaattcgc tgttccaaga
    21601 atcgattggc ggcaaatgat ctctcctcgt tatgagcaga cggactggta tgagcaaaaa
    21661 ataagagaag caaagcagat ccaaaaaaca tcgtttcttg atttatacgc tagcgagatg
    21721 ccagatgagc ttttgccgaa ttttgagttt ccttcttatt ctcttgaaga aatcttagcg
    21781 gaagaaaaag aaatggatct tttaggctat actccgttac gtaacaaaat caaacggtat
    21841 ttggaaagta aatttgaatt ggcattttcc cccaaccagc ttcttgtgac tggtggcggt
    21901 cagcaagcaa tctttttgat tttacaaacg attctttcag ctggagaggg gatagctgta
    21961 gagtcacctt ctttttttta ccgattacct cttttcaaag cttcaggaac gcgtttattt
    22021 gggataccga tggatgaaga agggatcgac ttagaaaaac tgaaagaatc gatccataaa
    22081 aataaattga aagcagtgct tatcaacccg aattttcaaa accctacagg aaaagtgatg
    22141 agtcaaaaac gacgagaaaa gctagtagaa ttgtgtcgac agtatcggct tccaattatt
    22201 gaagacgatg tatttacgga tctttcattt ctaaaccaag aagaagtcct tcctatacga
    22261 acgattgatc cggataatgt gttatacgtc ggttcgttat ctagggtttt aggaaaaaca
    22321 acgaaaatcg gttgggtgat aggacctgag acttttatct cgcaactagc tcaagcgcaa
    22381 gaaatgatgg agttttcgat gaatatcctt acacaaatca cagtggctaa tgtatttaat
    22441 gaagcaattg atttgaaaat gcaagatgta agaaaaaagt tgaaagaaaa aagccgactg
    22501 ataagcgctt ggggagattc acaatcgttt ttcaagcttc attcaatttt aggtggttac
    22561 tatgcctgga ttacttggga tgggaaaaaa ctagaccccg aactagcaga aaaagtagta
    22621 gatatgggtc ttggaattgc accaagtcta ctttttgggc aagatataaa tggaatcaga
    22681 atcaattaca gtaggctgga tgaaaaccaa ataccgttgt tttattcaaa gatgaaaatt
    22741 ctagaaaagt ggttaacaga ataatttcac aaagtaaata gattaaagaa tataacagct
    22801 tggatttaaa tttcaagctg tttctttttt ttcggaaaaa gaatacgata taaagaaact
    22861 aagaaaaaac cattatttgt acgtccgttc acgatgtgta aataaaataa atgtttgcaa
    22921 caaaactgta taacaccaac gtttttaaat agaaacagat ggttgtaagt ttgattagaa
    22981 aatagaaaaa atgcagatga aatataaaac gctttcctta ttgtgatttt attcactaat
    23041 gctgttaata tgcggattgg cataatttcc tttcatcgtg aaaatttgtg cttttagctt
    23101 gtatttctca taaatccatg ataatttatt aatgaagacg gttaaatagt gatacagttt
    23161 tattcactgt ttcaatcgca gaatgaagag attttcttat agatgagaga ggaatgtgtg
    23221 acaaatggca aacaaaaaag caccgatcga cttccaagca ctgctagatg aagtcaatgc
    23281 agatttccca gtatatcaag cacttgatca agatggaaac gtagttaaca aagatttagt
    23341 acctgattta tccgatgatg aactcgttga attaatgact catatggtat ggtctcgtgt
    23401 attagaccaa cgttcaacag cgctgaaccg ccaaggacgc ctaggctttt ttgcaccaac
    23461 tgctggacaa gaggcaagtc agctggctag tgcttacgca tttgataaag aagatgttct
    23521 tcttccaggt tatcgggatg tacctcaact agtaaaacat ggtttacctt tatctcaagc
    23581 ttttttatgg tctcgaggac atgctgcagg taattcttat ccggaagaat tgaaagctct
    23641 accaccacaa attatcatcg gtgcacaata tgtacaagca gccggtgttg cattaggttt
    23701 gaagaaacga aacaagaaaa atgtcgtctt cacttatact ggtgatggcg gttcatcaca
    23761 aggtgatttt tacgaagcaa tcaatttcgc tggcgcttac catgcaaatg ccgtatttta
    23821 tatccaaaac aacgggtatg cgatctctac acctcgtgaa aaacaaactg cagcaaaaac
    23881 acttgcacaa aaagctgtcg cagctgggat tcctggtatt caagtcgatg gaatggatcc
    23941 gcttgcagta tatactgtat ctaaaatggc acgggaatgg gcgatcagtg gaaatggacc
    24001 agtcctaatc gaaacactga cttatcgtta tggaccacat acactatctg gtgacgatcc
    24061 aacccgctac cgtacaaaag acacagatga cgattggcag aaaaaagatc cattgatccg
    24121 cttccgtaaa tatttgacgg aaaaaggact ttggtctgaa gaaaaagaag aacaagtcat
    24181 cgaagcaact aaagaagaaa tcaaagcagc tattgctgaa gccgacaaag taccaaaaca
    24241 aaaggtttca gatttcttga aaaacatgtt cgaagtatct ccacaatcaa tcaaagaaca
    24301 aattgcaatt tatgaagcga aggagtcgaa ataatcatgg cacaaaaaac aatgatccaa
    24361 gcaatcacag atgccttagc acttgaacta gaaaatgacg agaatgtcgt tgtttttggt
    24421 gaagatgttg ggaaaaacgg aggagttttc cgggcaactg aaggattgca ggaaaagttc
    24481 ggagaagacc gagtattcga tactccttta gctgaatctg gaatcgcagg attgagtttc
    24541 ggtcttgcgt tagaaggatt tcgtccagtt cctgaaatcc aattctttgg ttttattttc
    24601 gaagcaatgg acgaagtagt agctcaaatg gctcgtacaa gatatcgaat gagcggcact
    24661 agaaatctac ccatcacaat tcgttcgcca tttggtggcg gtgttcatac accagaattg
    24721 cattcggata atttagaagg tttgatcgct cagtctcctg gtattcgagt agtgattcct
    24781 tccaaccctt atgatgcaaa aggactattg atttcttcta ttagaagtaa cgatccggtt
    24841 gttttcctag aacacatgaa actatatcgc tcattccgag aagaagtgcc ggatgaagct
    24901 tacgaagttc ctttggataa agcagcagtt actcgtgagg gaacagatgt atctattatc
    24961 acatacggag caatggtgcg tgaagcaatc aaagcagcag acaatcttgc aaaagaaaat
    25021 atatctgttg aaatcattga tcttcgtaca gttgcgccat tagatgtaga aacgatcatt
    25081 caatccgtgg aaaaaactgg tcgcgtagtt gttgtccaag aagcacaacg acaagcgggc
    25141 gttgccgcgc aagtagtttc tgagatctca gaacgcgcta tcctttcatt agaagcccca
    25201 atcggacgag tttctgcacc tgatacagtc ttcccatttg gacaagcaga aaatgtctgg
    25261 ttgccaaatg caaaagatat cgaagacaaa gtaaaagaaa tcgcggaatt ttaagaaaag
    25321 aagggacgaa acaaagtcat ggcttatcaa tttaaattgc cggatatcgg tgaaggaatc
    25381 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
    25441 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
    25501 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
    25561 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
    25621 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
    25681 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
    25741 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
    25801 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
    25861 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
    25921 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
    25981 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
    26041 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
    26101 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
    26161 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
    26221 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
    26281 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
    26341 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
    26401 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
    26461 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
    26521 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
    26581 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
    26641 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
    26701 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
    26761 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
    26821 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
    26881 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
    26941 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
    27001 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
    27061 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
    27121 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
    27181 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
    27241 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
    27301 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
    27361 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
    27421 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
    27481 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
    27541 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
    27601 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
    27661 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
    27721 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
    27781 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
    27841 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
    27901 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
    27961 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
    28021 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
    28081 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
    28141 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
    28201 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
    28261 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
    28321 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
    28381 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
    28441 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
    28501 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
    28561 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
    28621 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
    28681 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
    28741 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
    28801 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
    28861 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
    28921 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
    28981 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
    29041 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
    29101 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
    29161 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
    29221 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
    29281 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
    29341 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
    29401 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
    29461 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
    29521 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
    29581 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
    29641 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
    29701 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
    29761 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
    29821 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
    29881 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
    29941 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
    30001 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
    30061 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
    30121 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
    30181 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
    30241 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
    30301 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
    30361 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
    30421 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
    30481 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
    30541 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
    30601 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
    30661 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
    30721 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
    30781 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
    30841 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
    30901 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
    30961 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
    31021 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
    31081 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
    31141 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
    31201 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
    31261 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
    31321 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
    31381 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
    31441 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
    31501 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
    31561 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
    31621 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
    31681 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
    31741 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
    31801 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
    31861 aagatgaacg acttattaaa acaatacttt ggctatgatg agtttagacc tggtcaaaaa
    31921 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
    31981 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
    32041 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
    32101 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
    32161 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
    32221 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
    32281 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
    32341 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
    32401 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
    32461 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
    32521 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
    32581 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
    32641 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
    32701 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
    32761 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
    32821 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
    32881 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
    32941 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
    33001 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
    33061 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
    33121 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
    33181 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
    33241 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
    33301 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
    33361 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
    33421 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
    33481 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
    33541 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
    33601 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
    33661 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
    33721 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
    33781 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
    33841 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
    33901 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
    33961 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
    34021 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
    34081 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
    34141 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
    34201 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
    34261 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
    34321 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
    34381 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
    34441 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
    34501 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
    34561 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
    34621 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
    34681 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
    34741 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
    34801 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
    34861 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
    34921 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
    34981 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
    35041 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
    35101 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
    35161 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
    35221 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
    35281 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
    35341 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    35401 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    35461 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    35521 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    35581 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    35641 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    35701 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    35761 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    35821 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    35881 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    35941 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    36001 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    36061 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    36121 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    36181 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    36241 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    36301 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    36361 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    36421 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    36481 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    36541 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    36601 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    36661 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    36721 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    36781 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    36841 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    36901 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    36961 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    37021 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    37081 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    37141 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    37201 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    37261 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    37321 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    37381 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    37441 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    37501 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    37561 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    37621 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    37681 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    37741 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    37801 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    37861 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    37921 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    37981 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    38041 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    38101 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    38161 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    38221 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    38281 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    38341 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    38401 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    38461 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    38521 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    38581 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    38641 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    38701 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    38761 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    38821 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    38881 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    38941 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    39001 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    39061 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    39121 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    39181 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    39241 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    39301 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    39361 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    39421 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    39481 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    39541 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    39601 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    39661 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    39721 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    39781 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    39841 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    39901 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    39961 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    40021 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    40081 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    40141 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    40201 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    40261 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    40321 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    40381 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    40441 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    40501 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    40561 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    40621 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    40681 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    40741 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    40801 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    40861 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    40921 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    40981 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    41041 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    41101 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    41161 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    41221 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    41281 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    41341 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    41401 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    41461 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    41521 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    41581 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    41641 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    41701 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    41761 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    41821 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    41881 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    41941 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    42001 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    42061 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    42121 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    42181 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    42241 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    42301 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    42361 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    42421 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    42481 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    42541 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    42601 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    42661 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    42721 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    42781 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    42841 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    42901 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    42961 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    43021 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    43081 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    43141 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    43201 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    43261 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    43321 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    43381 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    43441 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    43501 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    43561 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    43621 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    43681 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    43741 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    43801 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    43861 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    43921 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    43981 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    44041 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    44101 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    44161 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    44221 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    44281 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    44341 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    44401 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    44461 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    44521 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    44581 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    44641 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    44701 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    44761 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    44821 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    44881 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    44941 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    45001 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    45061 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    45121 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    45181 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    45241 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    45301 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    45361 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    45421 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    45481 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    45541 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    45601 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    45661 tttgtttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    45721 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    45781 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    45841 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    45901 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    45961 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    46021 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    46081 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    46141 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    46201 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    46261 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    46321 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    46381 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    46441 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    46501 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    46561 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    46621 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    46681 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    46741 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    46801 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    46861 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    46921 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    46981 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    47041 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    47101 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    47161 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    47221 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    47281 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    47341 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    47401 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    47461 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    47521 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    47581 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    47641 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    47701 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    47761 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    47821 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    47881 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    47941 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    48001 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    48061 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    48121 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    48181 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    48241 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    48301 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    48361 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    48421 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    48481 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    48541 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    48601 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    48661 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    48721 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    48781 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    48841 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    48901 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    48961 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    49021 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    49081 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    49141 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    49201 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    49261 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    49321 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    49381 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    49441 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    49501 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    49561 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    49621 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    49681 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    49741 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    49801 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    49861 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    49921 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    49981 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    50041 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    50101 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    50161 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    50221 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    50281 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    50341 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    50401 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    50461 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    50521 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    50581 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    50641 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    50701 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    50761 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    50821 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    50881 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    50941 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    51001 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    51061 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    51121 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    51181 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    51241 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    51301 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    51361 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    51421 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    51481 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    51541 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    51601 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    51661 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    51721 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    51781 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    51841 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    51901 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    51961 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    52021 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    52081 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    52141 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    52201 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    52261 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    52321 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    52381 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt
    52441 gttctggttt tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa
    52501 tacgatacca gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga
    52561 tagctaagac ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt
    52621 ccattttgaa tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa
    52681 acatgacagg aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa
    52741 tcaagataga tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga
    52801 cttggaataa cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac
    52861 gaggtgtttt gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca
    52921 aaacaatggc aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca
    52981 aaccaatgat gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat
    53041 cttttttttc taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa
    53101 cgacgacagc caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg
    53161 aactggcatc cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg
    53221 gcagccattc cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa
    53281 aaaacataaa ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg
    53341 agaaacccaa tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca
    53401 cgaagagaaa aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta
    53461 aaaattaaaa tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat
    53521 ataagacgta tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt
    53581 tacgctgcca tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa
    53641 aaaggcgatc gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca
    53701 gtgatgggcg atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc
    53761 cgcccaccca gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc
    53821 tgtggtaaac atttcatcat atgttttaaa attcaataca cgagggatca caccccacat
    53881 ttccatgtca tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat
    53941 aaaataagga atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat
    54001 tgcacgacca ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc
    54061 taaataatga atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac
    54121 tcgttcaatt gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt
    54181 cattttattc tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct
    54241 gttcaatcgt cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa
    54301 acagctgggt ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc
    54361 taccgatact tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc
    54421 tgacgcttcc tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag
    54481 tataagtttt catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat
    54541 catgtctacc gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg
    54601 ggctgacata aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat
    54661 cgtagtgagt atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt
    54721 gtttgattcg ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga
    54781 ccaaataaca gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc
    54841 ctccttgata gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca
    54901 aaaacaggta cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg
    54961 gatattctaa agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt
    55021 agattcatcc aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt
    55081 ttttatgttt ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag
    55141 agaaagaggt ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat
    55201 ttattttgcg acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag
    55261 gagcgagaat ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct
    55321 ttttaaaaac aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg
    55381 gtattttcaa aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat
    55441 ttgaagattt agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct
    55501 atatctatag tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga
    55561 cggcagaaga agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt
    55621 tttttaaaaa gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca
    55681 ctgtacgcaa tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc
    55741 agcgtgaact agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag
    55801 aagcacctac cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag
    55861 gggaagaaga gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag
    55921 ctgaagatgc tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga
    55981 cggcgaaaga caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat
    56041 atgccaatgg ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga
    56101 atcagatcac tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg
    56161 aactatcatt agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat
    56221 ttgatccaac tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt
    56281 ttttgattga cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac
    56341 tatcttatga aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc
    56401 atcgccgttt caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag
    56461 atcattggga ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc
    56521 aattaagcga aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac
    56581 aattgcttcc ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg
    56641 atcactacga aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta
    56701 ttgatccaag cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt
    56761 ctgctagttt ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg
    56821 cttacaaact gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc
    56881 tagagacgac ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg
    56941 cttttgctga tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag
    57001 atcttgcggt ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa
    57061 cggacatgaa tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg
    57121 aaacattgat tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag
    57181 gggaccgatt gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc
    57241 aggaactgat caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc
    57301 agcttcctgg aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg
    57361 atcaaggaat cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat
    57421 tcccacaaca ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa
    57481 tacagcgttt ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt
    57541 cagcctcgtt ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt
    57601 tacgtaataa agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct
    57661 gatcttcttg gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt
    57721 tcttcgcgtt ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac
    57781 ctagagcgtg ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca
    57841 gcttaccaaa taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa
    57901 ctagattgct gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg
    57961 acaattcttt tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata
    58021 caatgtcgaa gctagcatct tttgccatag gaaccctcct gatatacata taaatattta
    58081 ccatacatct atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc
    58141 tcatttgagt tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata
    58201 ccaaagtagg ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag
    58261 attaaagaaa atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta
    58321 ttttttgatt ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc
    58381 ctcgctttta tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt
    58441 gaatatccct gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag
    58501 ttttgtgaat gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac
    58561 tgataatatt ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt
    58621 atctttaaaa atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca
    58681 aacaactgga aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat
    58741 cgcaggcttc ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat
    58801 gactcaagta attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac
    58861 cgtcaagcca gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt
    58921 actgccgtct gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac
    58981 ccgctacgaa atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat
    59041 caatatttta ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat
    59101 gtatcgaaca actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat
    59161 caggtttatt ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt
    59221 aaacgagaat cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa
    59281 taaaaatcgt gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca
    59341 agaaatagaa ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga
    59401 attaggaatc gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa
    59461 tggagaaaca gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt
    59521 aacagaatct atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa
    59581 actagtctgg tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt
    59641 ggggatacga tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa
    59701 gccttttatc ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc
    59761 tgctatatta taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct
    59821 ttccttttgg aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat
    59881 cttcacgata aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt
    59941 tatttacaga caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg
    60001 ctaatccttg tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat
    60061 ggccagcgcc gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat
    60121 actcatgttc atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat
    60181 cgattcctaa agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat
    60241 agccataagc attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat
    60301 aagctttctt taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa
    60361 gtaaccttat tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt
    60421 ttatttattg tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata
    60481 aaaaaattat ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg
    60541 aattacttca gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat
    60601 ccaaatgaaa gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat
    60661 aaaatagtta ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa
    60721 cagatttttt caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag
    60781 atggcataag ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa
    60841 cacatgtaat cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta
    60901 gcttcaggcg ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt
    60961 gtttttttat aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta
    61021 aaagaatata ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt
    61081 gtgtgaaggt gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt
    61141 ctgatacaag aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa
    61201 aagaggtcat gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa
    61261 cgttcaaata atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc
    61321 caagaaccat aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag
    61381 tagtcaccat ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt
    61441 gctgtgtaag aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt
    61501 gctggagtag cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct
    61561 gcttgtgttt ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca
    61621 gcttggactg gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct
    61681 tctgttggga tggttaattg ttgtcctgag ta
//
LOCUS       DAYYGS010000004        60364 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000004 DAYYGS010000000
VERSION     DAYYGS010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.004"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..921)
                     /locus_tag="ACHA44_000227"
     CDS             complement(73..921)
                     /locus_tag="ACHA44_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8903554.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA44_000228"
     CDS             complement(1145..3085)
                     /gene="thrS"
                     /locus_tag="ACHA44_000228"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFM8903555.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3422..3601)
                     /locus_tag="ACHA44_000229"
     CDS             complement(3422..3601)
                     /locus_tag="ACHA44_000229"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HFM8903556.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3648..5471)
                     /locus_tag="ACHA44_000230"
     CDS             complement(3648..5471)
                     /locus_tag="ACHA44_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8903557.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5749..8028
                     /gene="helD"
                     /locus_tag="ACHA44_000231"
     CDS             5749..8028
                     /gene="helD"
                     /locus_tag="ACHA44_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase recycling motor HelD"
                     /protein_id="HFM8903558.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8121..8768)
                     /locus_tag="ACHA44_000232"
     CDS             complement(8121..8768)
                     /locus_tag="ACHA44_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903559.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8947..9666)
                     /locus_tag="ACHA44_000233"
     CDS             complement(8947..9666)
                     /locus_tag="ACHA44_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8903560.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9797..10948)
                     /locus_tag="ACHA44_000234"
     CDS             complement(9797..10948)
                     /locus_tag="ACHA44_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HFM8903561.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11165..11905)
                     /locus_tag="ACHA44_000235"
     CDS             complement(11165..11905)
                     /locus_tag="ACHA44_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8903562.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA44_000236"
     CDS             complement(11909..12232)
                     /gene="rsfS"
                     /locus_tag="ACHA44_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFM8903563.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA44_000237"
     CDS             complement(12289..12882)
                     /gene="yqeK"
                     /locus_tag="ACHA44_000237"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HFM8903564.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12866..13516)
                     /locus_tag="ACHA44_000238"
     CDS             complement(12866..13516)
                     /locus_tag="ACHA44_000238"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFM8903565.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA44_000239"
     CDS             complement(13526..13837)
                     /gene="yhbY"
                     /locus_tag="ACHA44_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFM8903566.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA44_000240"
     CDS             complement(13853..14962)
                     /gene="yqeH"
                     /locus_tag="ACHA44_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HFM8903567.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14959..15486)
                     /locus_tag="ACHA44_000241"
     CDS             complement(14959..15486)
                     /locus_tag="ACHA44_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HFM8903568.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15666..16451)
                     /locus_tag="ACHA44_000242"
     CDS             complement(15666..16451)
                     /locus_tag="ACHA44_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFM8903569.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA44_000243"
     CDS             complement(16444..17313)
                     /gene="accD"
                     /locus_tag="ACHA44_000243"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFM8903570.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17314..18693)
                     /locus_tag="ACHA44_000244"
     CDS             complement(17314..18693)
                     /locus_tag="ACHA44_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFM8903571.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA44_000245"
     CDS             complement(18701..19120)
                     /gene="fabZ"
                     /locus_tag="ACHA44_000245"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8903572.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA44_000246"
     CDS             complement(19145..19621)
                     /gene="accB"
                     /locus_tag="ACHA44_000246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFM8903573.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA44_000247"
     CDS             complement(19624..20859)
                     /gene="fabF"
                     /locus_tag="ACHA44_000247"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFM8903574.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA44_000248"
     CDS             complement(20878..21615)
                     /gene="fabG"
                     /locus_tag="ACHA44_000248"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HFM8903575.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA44_000249"
     CDS             complement(21627..22544)
                     /gene="fabD"
                     /locus_tag="ACHA44_000249"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8903576.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22651..22875)
                     /locus_tag="ACHA44_000250"
     CDS             complement(22651..22875)
                     /locus_tag="ACHA44_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8903577.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22928..23890)
                     /locus_tag="ACHA44_000251"
     CDS             complement(22928..23890)
                     /locus_tag="ACHA44_000251"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFM8903578.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23900..24340)
                     /locus_tag="ACHA44_000252"
     CDS             complement(23900..24340)
                     /locus_tag="ACHA44_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8903579.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24646..25611
                     /locus_tag="ACHA44_000253"
     CDS             24646..25611
                     /locus_tag="ACHA44_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8903580.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25668..25898)
                     /locus_tag="ACHA44_000254"
     CDS             complement(25668..25898)
                     /locus_tag="ACHA44_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HFM8903581.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25984..26871)
                     /locus_tag="ACHA44_000255"
     CDS             complement(25984..26871)
                     /locus_tag="ACHA44_000255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8903582.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(26960..28183)
                     /locus_tag="ACHA44_000256"
     CDS             complement(26960..28183)
                     /locus_tag="ACHA44_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8903583.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28200..29246)
                     /locus_tag="ACHA44_000257"
     CDS             complement(28200..29246)
                     /locus_tag="ACHA44_000257"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8903584.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA44_000258"
     CDS             complement(29410..31137)
                     /gene="ptsP"
                     /locus_tag="ACHA44_000258"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFM8903585.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(31137..31403)
                     /locus_tag="ACHA44_000259"
     CDS             complement(31137..31403)
                     /locus_tag="ACHA44_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HFM8903586.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31882..34116
                     /locus_tag="ACHA44_000260"
     CDS             31882..34116
                     /locus_tag="ACHA44_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8903587.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34264..34584
                     /locus_tag="ACHA44_000261"
     CDS             34264..34584
                     /locus_tag="ACHA44_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8903588.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34789..35013)
                     /locus_tag="ACHA44_000262"
     CDS             complement(34789..35013)
                     /locus_tag="ACHA44_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HFM8903589.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35211..36791)
                     /locus_tag="ACHA44_000263"
     CDS             complement(35211..36791)
                     /locus_tag="ACHA44_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFM8903590.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37192..38568)
                     /locus_tag="ACHA44_000264"
     CDS             complement(37192..38568)
                     /locus_tag="ACHA44_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8903591.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38696..39784)
                     /locus_tag="ACHA44_000265"
     CDS             complement(38696..39784)
                     /locus_tag="ACHA44_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8903592.1"
                     /translation="MFWSLELLIIAALIMISSEINFIFEPIGTFFSTLFAPVLIAGFL
                     YYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQISSLASNMPDF
                     IKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSLGSIVGTIASA
                     TVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTLANYISGQAIE
                     CLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLVTVFNDPVKAL
                     LCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLGIFLGVPFYAI
                     CKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            39956..40390
                     /locus_tag="ACHA44_000266"
     CDS             39956..40390
                     /locus_tag="ACHA44_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903593.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(40468..41001)
                     /locus_tag="ACHA44_000267"
     CDS             complement(40468..41001)
                     /locus_tag="ACHA44_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HFM8903594.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACHA44_000268"
     CDS             complement(41095..42273)
                     /gene="mutY"
                     /locus_tag="ACHA44_000268"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFM8903595.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACHA44_000269"
     CDS             complement(42266..43063)
                     /gene="recX"
                     /locus_tag="ACHA44_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFM8903596.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            43170..44552
                     /gene="rlmD"
                     /locus_tag="ACHA44_000270"
     CDS             43170..44552
                     /gene="rlmD"
                     /locus_tag="ACHA44_000270"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFM8903597.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(44726..45304)
                     /locus_tag="ACHA44_000271"
     CDS             complement(44726..45304)
                     /locus_tag="ACHA44_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HFM8903598.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            45626..46096
                     /locus_tag="ACHA44_000272"
     CDS             45626..46096
                     /locus_tag="ACHA44_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HFM8903599.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(46204..47430)
                     /locus_tag="ACHA44_000273"
     CDS             complement(46204..47430)
                     /locus_tag="ACHA44_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8903600.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(48184..48384)
                     /locus_tag="ACHA44_000274"
     CDS             complement(48184..48384)
                     /locus_tag="ACHA44_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8903601.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(48518..49126)
                     /locus_tag="ACHA44_000275"
     CDS             complement(48518..49126)
                     /locus_tag="ACHA44_000275"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFM8903602.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(49211..49681)
                     /locus_tag="ACHA44_000276"
     CDS             complement(49211..49681)
                     /locus_tag="ACHA44_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFM8903603.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(49729..50643)
                     /locus_tag="ACHA44_000277"
     CDS             complement(49729..50643)
                     /locus_tag="ACHA44_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8903604.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            50789..51592
                     /locus_tag="ACHA44_000278"
     CDS             50789..51592
                     /locus_tag="ACHA44_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HFM8903605.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            51770..52717
                     /locus_tag="ACHA44_000279"
     CDS             51770..52717
                     /locus_tag="ACHA44_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFM8903606.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(52785..53078)
                     /locus_tag="ACHA44_000280"
     CDS             complement(52785..53078)
                     /locus_tag="ACHA44_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HFM8903607.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYNPNLEEPTYHFKKQ"
     gene            complement(53106..53771)
                     /locus_tag="ACHA44_000281"
     CDS             complement(53106..53771)
                     /locus_tag="ACHA44_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HFM8903608.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(53911..54543)
                     /locus_tag="ACHA44_000282"
     CDS             complement(53911..54543)
                     /locus_tag="ACHA44_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8903609.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(54550..55617)
                     /gene="liaS"
                     /locus_tag="ACHA44_000283"
     CDS             complement(54550..55617)
                     /gene="liaS"
                     /locus_tag="ACHA44_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     LiaS"
                     /protein_id="HFM8903610.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(55614..56345)
                     /gene="liaF"
                     /locus_tag="ACHA44_000284"
     CDS             complement(55614..56345)
                     /gene="liaF"
                     /locus_tag="ACHA44_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HFM8903611.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(56514..56993)
                     /gene="greA"
                     /locus_tag="ACHA44_000285"
     CDS             complement(56514..56993)
                     /gene="greA"
                     /locus_tag="ACHA44_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFM8903612.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(57129..58304)
                     /gene="mltG"
                     /locus_tag="ACHA44_000286"
     CDS             complement(57129..58304)
                     /gene="mltG"
                     /locus_tag="ACHA44_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFM8903613.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            58508..59677
                     /locus_tag="ACHA44_000287"
     CDS             58508..59677
                     /locus_tag="ACHA44_000287"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8903614.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(59944..>60364)
                     /locus_tag="ACHA44_000288"
     CDS             complement(59944..>60364)
                     /locus_tag="ACHA44_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903615.1"
                     /translation="QMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSP
                     NKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVT
                     IRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT        17531 a        12646 c        10172 g        20015 t
ORIGIN      
        1 tgatggatca tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa
       61 atagtaaggt ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt
      121 cactttttcg ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt
      181 agcaaattgg gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct
      241 agatgtttgt acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa
      301 cgcaatgatt tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact
      361 tctacctgag tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg
      421 gagcaagacg tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg
      481 cagaatatct tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc
      541 cgattccaaa cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc
      601 ttttatcgct acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt
      661 tgcttctata tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat
      721 tttgaagtcc gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc
      781 actcaacccg gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt
      841 accgataatg tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc
      901 aatacgacta attacgctca taagttcctc caccctttta atgatagtct atgtaagtat
      961 accatatcag acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc
     1021 caacagaata tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg
     1081 aatacctgtt atctattgtt cggattgctt tctatctact acttttttac aggaaattta
     1141 cctattagtt ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat
     1201 tcaaatcttc tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt
     1261 ccaattcctt gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta
     1321 tcttatagcc cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca
     1381 aacgttcttt gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg
     1441 ttgcttgtat aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag
     1501 cgacaaaacg ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat
     1561 tttccccgtc ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt
     1621 gtatagttga taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg
     1681 gtccgtaaaa ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag
     1741 cggctttcag cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg
     1801 tattgtttgg atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat
     1861 atactgccac catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa
     1921 caaatgtatg accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac
     1981 ctgatttttc atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat
     2041 aagaatggat cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa
     2101 caagcatttc gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc
     2161 agtgtccaga tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt
     2221 aacctagact aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc
     2281 cttttggaag ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa
     2341 gttcttttcc tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt
     2401 ctttcaaagc tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt
     2461 ttgagtttcc acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc
     2521 cagtggatgg aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag
     2581 taatcacttc ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa
     2641 aaatttctaa tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga
     2701 cgattttcat catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc
     2761 cattatctgt atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat
     2821 ttggatagag gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc
     2881 caagtgcatc ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac
     2941 gagtcaaatc aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt
     3001 ttgaaatgct ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac
     3061 catctggaaa agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag
     3121 atcatcaagc tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa
     3181 aagttcgtgg ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc
     3241 cgaccgccct tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa
     3301 aagtttcagc ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca
     3361 tcaatcaatt atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa
     3421 cttatttttt ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga
     3481 catggatgtg ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca
     3541 tcatattttc taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca
     3601 taatgctctc tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt
     3661 gtcttgggta ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa
     3721 atagagaccg tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc
     3781 gtacttgcaa ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc
     3841 gctattctca tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct
     3901 ttcaagatga ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca
     3961 ataccgatat ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca
     4021 acaggtcttt gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta
     4081 cgggcatgta cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca
     4141 tccccggtga tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt
     4201 gctgcttctg ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt
     4261 gttacataga ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt
     4321 ccaatcttta cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc
     4381 agttgactaa caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag
     4441 atatggctag attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt
     4501 ccattgactg gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta
     4561 tcaaaagcaa tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg
     4621 atcccgtttt tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc
     4681 agtggacaag gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac
     4741 caccaagaaa taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca
     4801 gctaaacgga caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt
     4861 tgatactggc tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt
     4921 aaagaccctg acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc
     4981 aaagaagatt catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt
     5041 tcgccaggct tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc
     5101 aattttcctt ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt
     5161 tctttacccg ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc
     5221 aatatcatca aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc
     5281 gctaatacat ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc
     5341 aagaaggcga gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga
     5401 aaaacaaatt cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg
     5461 aaatgtgtca ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc
     5521 cagctgcctc tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct
     5581 taattgaaaa caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa
     5641 tattcagcaa ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact
     5701 atgctatact tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa
     5761 caactagaac aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg
     5821 ctggatacaa aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct
     5881 aatcacaaga ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc
     5941 caacatgagc aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg
     6001 caaacgttgt ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac
     6061 cgtgaaacag aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca
     6121 atcgttattg actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc
     6181 gcattttatg aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt
     6241 aaaatccaag aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac
     6301 tttttgctag agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg
     6361 atacaaaaag cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa
     6421 ggaatcgccg gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat
     6481 cataatcgca agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt
     6541 tctgattaca tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca
     6601 ttcaaaaatt atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa
     6661 gaaagtggtt ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc
     6721 ttagtcgatc agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt
     6781 gatatgaaaa taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa
     6841 gaaacaaatg aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg
     6901 aaaaaattag gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg
     6961 gaagaacagc tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag
     7021 gagcgttcat tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc
     7081 catcgaggga tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta
     7141 caaactgtgc cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa
     7201 catttgacaa ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta
     7261 ctattctttt tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat
     7321 atctttattg atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg
     7381 ttcccaaaag caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa
     7441 acgatcgttg gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta
     7501 acgacaagct atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa
     7561 gaacatcaag tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca
     7621 acagatgata tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc
     7681 cggtattggc gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct
     7741 caactgccag acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg
     7801 aagcgttcca ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt
     7861 ttcttgtggg atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac
     7921 accatgtgta cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct
     7981 ttgttaaaca atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa
     8041 ctgctatcat taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta
     8101 attcaatcat ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt
     8161 aatttgacaa gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca
     8221 aataccttgc tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat
     8281 tcaatgaatt ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc
     8341 tctgatacac gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc
     8401 ttctcatttc tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt
     8461 agaaagaaag cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga
     8521 cgaaaaagca gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc
     8581 atacactcta aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct
     8641 tcaacgccga atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct
     8701 aactcttcta ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt
     8761 gcttccatat tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta
     8821 tgctctcatc ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga
     8881 caatctgtta gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact
     8941 ggttgcttag tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc
     9001 cagtgcatca atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt
     9061 ttgagggatc atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc
     9121 ttcttgtata ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc
     9181 cattacatcg cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc
     9241 ttctcctgca aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac
     9301 acccatgttc ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac
     9361 attgttcgtc aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg
     9421 caattcagag tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat
     9481 atgttttggt acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc
     9541 atgcggatca ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata
     9601 aatccggcta ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg
     9661 tcccatattt tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc
     9721 cattatactt gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc
     9781 atttttagtc aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat
     9841 tgcctcccaa ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt
     9901 tttttccgat tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag
     9961 catcggtaaa tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct
    10021 ttttgatatt cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt
    10081 ttgtttttac ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca
    10141 tgcgcgattc tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag
    10201 ataacaaccg ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg
    10261 aaagatcttc ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg
    10321 catggcgaat ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc
    10381 ctgcactttt acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct
    10441 ttgcataact taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat
    10501 acagactgcg aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt
    10561 cttgaaatgc ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt
    10621 aatcaaacga agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca
    10681 agatttttac tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa
    10741 gctccactac taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga
    10801 tgatagccgg ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg
    10861 ctcctgttat ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt
    10921 actctacgat aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa
    10981 gaaatagaga caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta
    11041 ttctcgcaaa cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc
    11101 gttcggatat ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa
    11161 accgctagtc tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt
    11221 ctgtaaaatc agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat
    11281 aactttccaa tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag
    11341 acccttcttg ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc
    11401 ctggataact atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg
    11461 gaaaaacctc atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt
    11521 ctaacgcttg atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc
    11581 acagcgaatc agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca
    11641 tattgccttc cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca
    11701 tcaacatctc ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa
    11761 ccgctaatgc tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat
    11821 gacgtttcga aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa
    11881 tgaaagcaaa tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg
    11941 gagcatctga ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat
    12001 gtacgatcac atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac
    12061 gttttacttc atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga
    12121 tttgacgttc gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc
    12181 gaacgtctaa agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt
    12241 ttaattgttc tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc
    12301 tacccagtaa ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa
    12361 atgggccaat gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat
    12421 ggctcgtgca tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata
    12481 aatgattttg tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg
    12541 aagaatctct gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc
    12601 atgccagatt gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac
    12661 cattttgaac tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact
    12721 agctttttca ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc
    12781 aagcacatgc ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc
    12841 tctagaataa gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa
    12901 tataatcaat tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt
    12961 gacgtatttt cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg
    13021 gagtatccgt tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct
    13081 catcgatttt ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat
    13141 aatctgtatc cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc
    13201 caccacgagc taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt
    13261 ccaacatgtt gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct
    13321 gataagaagg catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca
    13381 tcactaaatg tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt
    13441 tcttctctaa gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag
    13501 cttgcgtatt catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat
    13561 acttttcttt tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt
    13621 cgcaatctat ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt
    13681 gaagtaacga tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca
    13741 ttgctgaatt gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc
    13801 tacgtaaaaa acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca
    13861 atgctttgcg tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg
    13921 ctgttacagt gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa
    13981 actcaaaacg aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata
    14041 atccacctac atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat
    14101 ttacatcatt tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc
    14161 ttgcaagtcc gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga
    14221 tttcttttaa cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat
    14281 ggcgatggat aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga
    14341 ttttatccaa ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg
    14401 tatttttgat gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat
    14461 agacatcccg tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag
    14521 atttcttagc actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt
    14581 ccttcatcca ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt
    14641 tcccaactaa aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac
    14701 cattgaagtc aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat
    14761 tcagtaagcg caaaaagtcg tcatccgtca atgagacatc ttgggtctca ttataatggc
    14821 gtaatctgaa acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg
    14881 tttgcggcgt gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga
    14941 tacattgtaa ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc
    15001 taatagatag gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc
    15061 tgttgcgata atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat
    15121 atcagtcata atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt
    15181 ctttgctatg ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc
    15241 aaatttttca atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg
    15301 gattcctgca tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt
    15361 attccatgca atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt
    15421 taattgggca ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga
    15481 aaacatgctg tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc
    15541 gtattatact gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa
    15601 gcatttcttt acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa
    15661 aacaactaat attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat
    15721 cgaacaagtt ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat
    15781 cgtggcaact gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg
    15841 cgtagttctg gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct
    15901 ttccacaaaa tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc
    15961 atccagactt catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg
    16021 atgatggcga tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc
    16081 tctccttcgc cgcgctcttc tgctcctaca ccgcaataag cacctgctgt attgatcaag
    16141 gtaatcaccg gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag
    16201 ccttcaggat ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt
    16261 tgaataccga tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct
    16321 tcgtcatctg caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca
    16381 aaaaaatcca agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct
    16441 gtcttatcca cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt
    16501 ctgtttcaat tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc
    16561 agccttttgg aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc
    16621 agcaaaaccg atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact
    16681 ggcagttact cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt
    16741 gctatgccgt ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc
    16801 ttgcatccgt gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc
    16861 tcgttcgaat aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa
    16921 attaggatcc atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag
    16981 gactgcttca tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg
    17041 aaatgctagc ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc
    17101 aataatcaaa gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca
    17161 tactttttct tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc
    17221 ccacatgtta tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat
    17281 ccgaatatat ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg
    17341 tgtccaattc ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac
    17401 tgcaggatga ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat
    17461 aaattcactt aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac
    17521 gattattttt gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc
    17581 actgtctact cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg
    17641 agaaggagca aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc
    17701 ttggaatact acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg
    17761 tttttttact aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg
    17821 agtattcatt tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc
    17881 ggcattttca taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct
    17941 ttttgtttgt gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg
    18001 taaagaacaa tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg
    18061 aacttctata tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa
    18121 tgccgctgct gcttcttgct gagctgaaga aaaatgctgt gggagctctt cttttgattg
    18181 aactttccga atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc
    18241 aattctgtct gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg
    18301 aatgactggg acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc
    18361 atcgatcgtt gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc
    18421 aaatcggcta ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac
    18481 gattgcagcg ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc
    18541 aatgcagatt gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata
    18601 tatcgctacg gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc
    18661 accgcggtta gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta
    18721 attccgcttc ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg
    18781 aagctttcac tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct
    18841 tacggaattt tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa
    18901 gagaaagtaa tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca
    18961 taacaggttc ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct
    19021 tagcaacgat ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat
    19081 aacgatgtgg aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct
    19141 ttgcttattt ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt
    19201 ctacaaggat ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca
    19261 tcgcttcaat aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag
    19321 ctggtttgtc gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga
    19381 ttccatcaaa tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt
    19441 caggtatgac attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca
    19501 tgcctcgact tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga
    19561 attccgttag cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca
    19621 aaattattcc tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga
    19681 attactcatt gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc
    19741 aatctcagga tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc
    19801 cagcaaagtt gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga
    19861 tttcgtgctg ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt
    19921 tgcttctgcc tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg
    19981 ttccggctgt acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc
    20041 atctggagta ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc
    20101 tccatagatt ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc
    20161 tgctccttca cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt
    20221 aggatcagtt gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca
    20281 aatcgttgct tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg
    20341 gaatgcttcc ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat
    20401 accttttgca cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg
    20461 tacgaacatt ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc
    20521 gattgttgga agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat
    20581 tttttcttta tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat
    20641 cgaaaacata tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc
    20701 tttgacttca cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc
    20761 gatgccattc gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg
    20821 tgaagtgact ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta
    20881 tccgtgcatg accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg
    20941 tgctaagaaa actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat
    21001 ttgcttttcc gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat
    21061 gaatcctggc gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga
    21121 ttttgttaat cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc
    21181 aatcaaccca gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt
    21241 tttcaatacg tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt
    21301 gaaatcttct gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac
    21361 taacacatgg atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc
    21421 ataatcagag atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc
    21481 tatcttttct tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc
    21541 aaaagctaag gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt
    21601 tccttttaca tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc
    21661 ttattctcaa cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat
    21721 acttttccgg ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata
    21781 ctctcataga aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct
    21841 ttcttcatta tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga
    21901 atctgactta ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa
    21961 ggcccgctga catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca
    22021 gcttcgtcta ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt
    22081 gcaggagata cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc
    22141 ggcgcattca tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa
    22201 gcgcctcttt ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact
    22261 aatgccgaat attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc
    22321 ttcaataagc gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc
    22381 tgatccaaac gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca
    22441 cttgcttcat caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct
    22501 tgatattgtg caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata
    22561 tccagaatat atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa
    22621 atgtcccagc ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac
    22681 taaatcttga accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc
    22741 aatttcattg atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc
    22801 agtagtcaat ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat
    22861 tttttcgaat accataataa tatattcctc cgatattgtt tgctttttat ttttataatt
    22921 taacagtcta caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa
    22981 atattacttt ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata
    23041 agatagggat gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt
    23101 cttgcggcat ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca
    23161 aaagcagata atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc
    23221 gtacggcaaa atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat
    23281 cttctgaata gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg
    23341 taccgtcagc tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa
    23401 tcccacctgc tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct
    23461 tagatagtgt ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt
    23521 cagccatgct taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag
    23581 catgagttgc tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg
    23641 gcgacattgt tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta
    23701 tcaatttttc cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc
    23761 gttcacgaat acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa
    23821 gatcattatt agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa
    23881 attgttccat taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg
    23941 catccagcaa ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt
    24001 cacgatgaaa atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact
    24061 ttacaacgcg gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact
    24121 tgttaatagc agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag
    24181 ttgttttttt cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat
    24241 ctttgaattg agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac
    24301 taacaagata atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta
    24361 gtttgattat caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc
    24421 ttaaaagatc atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac
    24481 tgataaatca gcatttataa tttcttttat tagaaaaatc atctatctca catattttga
    24541 ttatcaaagt attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc
    24601 aaaatttgtt atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa
    24661 atgaaaactc tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc
    24721 atctatgttg gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat
    24781 aaacagcatt caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc
    24841 gtagtagaca cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt
    24901 cctttttata accgacaaat gaattatttc agtaattata attatgtaca tattaccaat
    24961 aagggatttg tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat
    25021 aaaaaaatgc cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct
    25081 gaaattagtt tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa
    25141 cagaaaaaaa aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac
    25201 ttgaaaaaaa gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt
    25261 caaaaagata tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt
    25321 ttaagaacac catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag
    25381 cactacctcg tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct
    25441 cctgacttct attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa
    25501 gaagaaatcg atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag
    25561 tttgaaattg aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc
    25621 ggcttctttt aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag
    25681 tcatagattt ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct
    25741 gtttcagtta attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg
    25801 ccttcttttt caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc
    25861 agtcgtttta tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt
    25921 tctgttatat tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt
    25981 tttttatgat tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg
    26041 ggttccgtca ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt
    26101 agttgctggc aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt
    26161 gataaaatgt ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata
    26221 attgcttaaa gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa
    26281 atcaagtccg tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc
    26341 aatcatgatc tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt
    26401 aaatgtcgta ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc
    26461 accgaccatg atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc
    26521 atctaacgca tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg
    26581 tgtacttgta gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga
    26641 ctgtccatag tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt
    26701 actttgttct gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg
    26761 atcagctttt gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg
    26821 gcgtgcgaca gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac
    26881 ctcctaaaaa tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt
    26941 cataaaaaat gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa
    27001 tcgagtcttc ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat
    27061 aaaattcgat catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta
    27121 aaacttttgg atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt
    27181 cttcgtcggt ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag
    27241 catttccttc cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt
    27301 gtgtttctga agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat
    27361 cattgttgtc cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca
    27421 attcctctaa ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat
    27481 aagcggagat gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa
    27541 tcctgcttaa agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt
    27601 tgctgatatc ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag
    27661 gaatcacgcg aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac
    27721 ttggacaaac tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg
    27781 attgcctgat cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt
    27841 aagtatggat cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc
    27901 cgaattctgt atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat
    27961 accacatgcc tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca
    28021 ttcgaatcac atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga
    28081 cttcatgccc atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc
    28141 tgacttgcgg aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac
    28201 tagtccttga cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt
    28261 tcgtatacat ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga
    28321 taaccggctt gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata
    28381 aattcttcgt ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac
    28441 actggaatat ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc
    28501 ccttctgtct cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct
    28561 atgatctctc cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca
    28621 attttccgga tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg
    28681 tattcgggca tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac
    28741 aaaccgacac aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt
    28801 tgttctttca ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga
    28861 atcggcacac ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc
    28921 agatagtccg ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg
    28981 ttcgatctga taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact
    29041 ttttttcctt ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg
    29101 atgtgcagga tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc
    29161 agagcacgtt tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca
    29221 ctttcaaaat ataataatac cttcacctta cgtccccctt cacttatata gtataacata
    29281 gcctacaaac tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg
    29341 ttttcttaaa aaagaacaaa aaaactggcg tagctctttt acaagccaca ccagcttttg
    29401 atacgttgtt tattttacat attcatgaac aagttcaacg acttcttcca tcatatcgca
    29461 ttctttcaat gcacgatcag caagttcagc cattttcgct gtgtccaagc gtttcattaa
    29521 actacgcgtt ttaagaatag atgtcgcact cattgagaac tcatctagac ccatacctac
    29581 tagcaatgga acagctgttt gatctccagc catttctcca cacatacctg cccattttcc
    29641 ttcagcgtgt gctgcatcaa ttacattttt gatcaaacgt aaaattgatg ggttatatgg
    29701 ttggtacaag taagaaacgc gttcgttcat acggtctgct gccattgtgt attgaatcaa
    29761 atcgttcgta cctacagaga agaaatctac ttcttttgca aacttgtctg ctaagactgc
    29821 tgctgctgga atctcgatca tgatgccgac ttggatcgta tcagatactt ccttgccttc
    29881 acttatcaat ttttgttttt cttcttcaaa gatcgctttt gctgcgcgga attctttgag
    29941 tgtagctacc atagggaaca tgatacgtaa attaccatga acagatgcac gaagcaatgc
    30001 acgcatttgt gtacggaaca tttcgtcgcc ttgttcagat aaactgatac gcaatgcacg
    30061 atatcctaag aatggattca tttcatgagg taattgcaga tatggtagtt ctttatctcc
    30121 accgatgtcc attgtacgaa caactacagg ttttccttcc ataccttcta atacagcttt
    30181 gtaagcttca tattggtcat cttctgttgg gaagtctgga gaatccatat acaggaattc
    30241 agtacggtac agaccaactg cttctccacc attattatgg acaccaacta gatcttttgg
    30301 tgttccgatg tttgcagcta attcaaaatg cttgccgtca gctgtgacag tttctgcatt
    30361 ttttaatttt tcccattctg ctttttgtgc tgcgtaatct gcacctgctt tttcaaattc
    30421 agctttttgt tcatctgtag gatcgatgat tacatcgcct tcgatcccgt tgacagccaa
    30481 aatatcgcct tctttgactt tagcagtaat ttcttttgtc ccaacgattg ctggaatttc
    30541 taaagaacgt gccatgatag cagaatgtga agtacgtcca ccaatgtctg ttacaaacgc
    30601 ttttacaaaa ttgcgatcta attgtgctgt atcacttgga gtcaagtcat gtgcaacaac
    30661 gaccacttct tcgttgatca ttgatggatt tggtaaagta acgcctaata agtgcgctaa
    30721 aatacgttta gcaacgtcac gaatatctgc agcacgctct tgcatataag cattatcttc
    30781 cattgcttcg aacataccga tatacatatc cgttacttct tttagcgctg attcagcatt
    30841 tacgctgttg tctttaatat tttgcttgat ttgacctacc atttctggat ctgaaagaac
    30901 catcaaatgg gcatcaaata cttgcgcttc tgcttcacct aagctttgcg ctgctttttc
    30961 acgaatttgc tgcaattctt cagtagattt agctaatgca gcatccaaac gagcttcttc
    31021 agccgcagta tcttctactg ttgccttact gaatgttaaa tccggttgaa ctagcaggta
    31081 agcttttgca acggctacac cgtcactagc ggcaatccct tttagcattt caaccattat
    31141 tcagataagc cttctttctt cattgtttca acgattgctg ccattgcatc agcttcgtca
    31201 gcaccttctg cagtaatagt aacatcagaa ccttgaccta cacctaaaga cataacgccc
    31261 atgattgatt tcaagtttac tgatttacct ttatactcta agtttacatc agagttaaat
    31321 ttgcttgctg tttgtactaa tagtgtagct ggacgagcgt ggatcccagt ttctgctact
    31381 acgtgaaatt ctttcttttc catattaatc ggtctccttt aagtgagtgt tgtttttatg
    31441 tgagggtttt cttttttagt aattcagaga atcctaacaa atgccaccct ttcatctgta
    31501 aagattaaca ttttttttcg ttagatacaa cagttttcat tcaatttcag ataaaagtat
    31561 tcaaattatt ccggattctt tttcatcctt ttcttcacat tttataacaa acgtgtttat
    31621 ttcctttttc ttgtcgcttt ctcttctcct gacgtttatc attctttcct ttgaccctct
    31681 ttgttttcat atgaaacgaa ttacttgctt ccctttttat ttttagtata ataagagtca
    31741 aaggtcagga atggtcaata gagatttcac cttttctacc tcataaatct cttaaaaaac
    31801 taggggttat tcttttacct catgaaaaac gagatgtaaa atatggatgt acccattttt
    31861 gtgaaaggac gtgaatgatt catgctttgt caaaactgcg gcaagaatga ggcaacgatt
    31921 cacttgtatg ccactgtcaa tggtcagcgg acacaactcg attactgtca gagctgttat
    31981 caaaaaatca aaaatcaaca aaatggaggt ctgactaata tggcccaaaa tgatccattt
    32041 ggatttggca gtttggacga tcttttccgt tccatgtctc gccaaatgca acagcaagga
    32101 aattatgagc aaacaccacc aacacaattt ggcggaaaca acaacttcaa tggtggtcag
    32161 ccacctcaag gcaatacaga aggtttattg ggagaatacg gaatcaatat cacagaacaa
    32221 gcacgacaag gtgatatcga tcctgtgatc ggacgcgatg acgaaatcaa acgagtcatt
    32281 gaaattttaa atcgccgaac caagaacaat cctgtcttga ttggagaacc aggtgttggt
    32341 aaaacagcag ttgttgaagg tttagcacaa aaaatcgtag atggtgatgt accgcaaaaa
    32401 ttaatggaca aagaagtcat tcgtttagat gtcgtatctt tagtccaagg tactggtata
    32461 cgaggacaat ttgaagaacg tatgcaaaaa ttgattgaag agatccgtca agcagaaaat
    32521 gtgatcctat tcatagatga agttcacgaa atcgtcggtg ctggttctgc tggtgatgga
    32581 aatatggatg ctggtaatat tttaaaacca gctttagctc gaggagaatt gcaaatggtt
    32641 ggtgctacta ccttaaatga gtaccgcatc atcgaaaaag acgctgcttt agaacgtcgt
    32701 atgcaacctg tacgggtaga tgaaccaact gtcgaagaaa cgatcagtat tttgaaaggt
    32761 ttgcaaaaac gttatgaaga ctatcatcat gtaaaatata ctgatgaggc catcgaagca
    32821 gccgcaactt tatccaatcg ttacgtccaa gatcgcttct tgccggataa agccatcgat
    32881 ttgctagatg aaacaggttc taagaaaaac ttgacgatcc aaatcgttga tcctaaaaca
    32941 atcgagaaaa aactgcaaga agcagaagaa caaaaagttt tagcttctcg agaagaagat
    33001 ttcgaaaaag cagcttatta tcgcgatcag atcaataaac ttcaaaaaat gaaagaacgt
    33061 cagctcactg aagaggaaac acctgtgatt accgaaaaag atatggaaaa aatcgttgaa
    33121 caacggacag gtattccagt gggtgaatta aaagaaaaag aacagacaca actgaaaaac
    33181 ttagcagatg acttgaaagc tcatgtcatc ggacaagata acgctgtaga tcgagtagcg
    33241 aaagcgattc gccgaaaccg tgttggatta aacaaacaga atcgtccaat cggctccttc
    33301 ttatttgttg gaccaactgg tgtaggtaaa acagagcttg cgaaacaact tgcctacgaa
    33361 ctattcggtt cacaagattc aatgattcgt ttcgatatgt ctgaatatat ggaaaaacat
    33421 agtgtctcta aattgattgg ttctcctcca ggttatgtcg gatacgaaga agctggtcaa
    33481 ttaacagaaa aagtacggag aaatccttac agtcttgtct tgttagatga agtagaaaaa
    33541 gctcatccag atgttttgca tatgttcttg caaatcttag atgatggtcg attgacagat
    33601 gcacaaggaa gaacggtcag ctttaaagat acgatcatca ttatgacaag taatgccgga
    33661 actggtaaag tagaagcaaa cgttggtttt ggtgcggcac gcgaaggtgt tactcgttct
    33721 gtattgaacc aattaaacaa ctactttaca ccagaatttc tgaaccgttt tgacgggatc
    33781 atcgaattca gtgcattatc aaaagaaaac ttgatgacaa tcgtcacact aatgctagac
    33841 gatgtcaacc aaatgctggc agcacagcaa cttcacatcg aagttcctac gaatgtcaaa
    33901 gaaaaattag ttgacttagg ctatgatcca tctatgggtg ctcgtcctct tcgacgcacg
    33961 atccaagaac aaatcgaaga tggtatcgct gaattttatc ttgatcaccc ttctatccat
    34021 gagttgaaag caaaactgga taaagatgga aaaatcatcg ttacttctaa accagaacgt
    34081 ttggctaaag aatcagccga agaaacggct gaataaatcc agttttatag cgtgagacgg
    34141 tatttctttg agaaatgccg tctcattctt ttttcagaaa agtcaaaata ctgttcattt
    34201 tataaaaaag aaattataat aagagaaaca agcaattaaa aatacataaa agaggtgagc
    34261 gccatgaaat tggtaaatgt aacaaacagc cattcccgtc ttgtattaaa ccagcttgaa
    34321 aacactgatg cccatctagt caaggtctac acagccggaa ataccactat tgtctacact
    34381 gaagcccctg aacataatga aattctgctg atcaatgaca aacgtaaaat ccagccgaaa
    34441 gaaatcgaag atgctaaaaa ttatttctta gataaaatca aagacgccgt ttaccacgag
    34501 gatgaaatca aagtaatcga attggcaggc cttgttgaaa tctcgattcc aaaacatgaa
    34561 ttaggcgcta cattagccgt gtaaacaaat tttgaacata ataattaaag agcgtgggac
    34621 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcga aagcagaagc
    34681 aactccttcg gaaataagct gaaattcacg aaaattgctt ttcaaatttt tgtgaattcc
    34741 agcttatttc ttggagttaa acgcttctgt ctcagcctct ttttctttct agctttttga
    34801 tttttctctt ttcttttgta cttttctctt ttcattttca gttctacgcg tgtctttatt
    34861 tttaactttt tcctctttat ggatgattga tcgtactttt tgtgcaattt gctcgttttt
    34921 gtcccagtct tttacatcta ataatcgaca taatcgtaac atctcttctt ttttctgttt
    34981 ttttctttca cgtctttgcc attctgtttc catgtgtcac actctccttt ctttaatcat
    35041 atgtacgcaa aataattttt atattttttt gattagacat ttctatgatt ttaatatagt
    35101 aaaaagagcc gtcacggaaa atccgtcacg gctctaaaca aaatatgaga ggctgttttt
    35161 gtttggctat tcttattact tgatgcaggg cagcttgtgt caagacttct ttacatcaaa
    35221 ctttttaatt ttacattggg atatttatcc gcaaaccagc gttctgcaaa ttggttctcg
    35281 aataagaaca acggttggtc gaaacggtca cgagccaaga tattgcgact tgaactcatc
    35341 ttctcatcca aatcttctgg atcgatccag cgagcgattt tgtgtcccat tggagacatg
    35401 attacttcgg cgttatactc atttaacata cgatattgga atacttcgaa ttgtagctga
    35461 ccaacagctc caatgatata ttcttccgtt agatatgttt tataaagctg aatcgctcct
    35521 tcttgaacta attgatagat tcctttgtgg aaggattttt gtttcatgac attttttgca
    35581 gtcactttca taaacagctc tggagtaaat gaagggagtt cttcataagc aacttttagt
    35641 tttccttcga acaatgtatc cccaatctga taattgcctg tatcatatac accgatgata
    35701 tcgcctgcta ctgctttttc gacgttttct ctcgaatctg ccataaattg tgtgacattg
    35761 cttaatttca gttttttatt tgtacgttcc aaccagacat ccattcctcg ttcgaatgtt
    35821 ccagaacaga ttcggacaaa agcgattcgg tcacgatggg ctggattcat atttgcttgg
    35881 atcttgaata cgaaacctga aaattcttcc tcataaggac ttaccatctg atcatcttgt
    35941 gttttgtgtc cataaggagc tggtgccaat tcaacgaaag tctctaaaaa agtctgtaca
    36001 ccgaagttag tcaatgcaga accaaaaaat actggtgtct gatcgccacg agcaattttt
    36061 tcttgattaa atgcatctcc agcctctttg attagttcta cttcttctaa gacttgttca
    36121 tataaggaat tttcagttag aggatgattt tcgggaagca gtccatcttc atccaattct
    36181 actaatcgtt catcctgata attttccgga cgataaaact ctacacgatt atggtagata
    36241 tcataaagac cttctagccc tttacccata ccgattggcc aattcatcgg ataagattcg
    36301 atatctaata gctcttctaa ttcttctaac aattccaatg gttcacgacc atcacgatct
    36361 agtttgttga taaaagtaaa tatcgggatg ccacgttttt ttacaacttg gaataatttc
    36421 tttgtctgtg cttcgatccc ttttgcacta tcgattacca tgaccgcact atccacggcc
    36481 atcaaagtac gataagtatc ttcagaaaaa tcttcatgtc ctggtgtatc caagatattt
    36541 actcgtttcc cttgataatc aaactgcata acagaactag taacagaaat cccacgttgt
    36601 ttctcgattt ccatccagtc tgatttggca aaattacctg tttttttgcc ttttacggtc
    36661 ccggcttgac ggatagcccc accaaacaat agtaattgtt ctgtaatcgt tgttttccca
    36721 gcatccggat gggaaatgat cgcaaaagtt ctgcgattgt ccacttgttg ctttaatttt
    36781 ggattattca taacctaact ccatttcttc cattcaatta tttatatttt ctctaggcac
    36841 agattgtccg atctcttaaa gaaagacaat ttcatatagg gctgtgacaa aagccgtgta
    36901 gcagtctctt tttccgagta aacggcgttc tagagacagc ctatcagcaa taagcccacc
    36961 caagccaaaa atacgaaaag ctgtttttgc ttggctgctc ttattactta atgcaggata
    37021 gctttttcac aacctttgtc aactactaca actgaatcag tatagcatgg attcagcgaa
    37081 tgaaaaagcc accaaaccat aagagtcaaa tatttttttg tcagctgact aaaagaaaag
    37141 acgtgttgct tgtttattca agcaacacgt ctttcataac caagtttcca attatcttct
    37201 tggctcgtca tcttcgaatt cttttctgaa gtatcttcgt tcttcttcag gttccgcttc
    37261 tgtttcttcc ggatcatgaa aaatgacacg gattttaagg attcgagaac cttccatttc
    37321 ttcagaaacc agcgtaatat tatcgacatc aaacgataac ttttcgcctt catccggaat
    37381 catgcctaat gctgtaatca aataaccagc catcgtatct acatcactca tatgcagatc
    37441 actgtcaaaa gcttcattga actcatcaat cagcatacgt ccttggatga tatattcgtg
    37501 ttcatcgatt ttttcataaa gattctcaac ttcatctgat tcatcatcga tttctccgac
    37561 gatttcttct aaaaggtctt ctagtgttgc taacccaacg acgccgccat attcatctaa
    37621 taaaatagcc atctgattct gcgtcttttt catttcatat aacagatcat caataaagac
    37681 cgtttcagga acgaataaag gctcctgcat gattttcttg atatctaaat tatcaaagcc
    37741 gaatttatgg gcagctttta gtaaattctt tgtatgaagg attccgacta ctttatcttt
    37801 gtcttcgtta taaacaggga ttcttgaata gttttcggat aaaacttcat tcacattttc
    37861 ttccacagtg tcgttgatat caatcataaa tgcatccgtt cgtgggacca tgacttctcg
    37921 ggcaactttc gtatcaagag aaaaaacccc ttggagcatt tctaattctt cattattcaa
    37981 tacgccttcg ttttcaagca tataacgcat ttcatctctg gtcatttttg aatcttcatc
    38041 atcaaaagtc atcggcgtga tacgcgccaa taaacttgtg gaagcagaaa gcagccagac
    38101 gaagggctta gctactacac caatcaagcg tatcagccct gaagtgaact ttgcgacttc
    38161 ttctgattta tttaaagcaa tacgtttagg gtacaattct ccaaaaacaa tcgatacata
    38221 ggtcaaaata gccagcacaa tgatatttgc aatatttttt gccgctgtgc cgccacctaa
    38281 taccggagct aaccgttccg ataaagtatt agctagtgaa gcaccagaca aaatgttgac
    38341 caaagtgatt cctacttgga ttgtcgatag gaaattattt ggattctgca atatttttaa
    38401 aagcttttga gatttcacat ctccttcatc agctttttgc tcgattcgat tcttattgat
    38461 cgaaacaaca gcaatttcgg aagctgctaa aaaagcattg atcaatgtca aaacgatcaa
    38521 taataaaatc tgcgctaaca gcgactgact ctcagggtca gcattcataa tcgcattctc
    38581 tccttataaa attaattttt agtttacgat agaaaaaaac gtggcactat agccacgtta
    38641 aaatggtatc acaaagcaat tttctttgca ataaaaacaa gtgtactaaa actacctacg
    38701 aggtcgtagc aattttcttc ttgttttcat tttgcttgtc ttctttgacg attttgatta
    38761 tataggaaat gatcgtcttg cagattgcgt aaaaaggaac acctaagaag ataccaagca
    38821 atccggcaat atttccagct actagaagaa cgataatgat cgttaatgga tggattttca
    38881 gtgatttccc aattacatta ggataaatga tattcccatc caactgttgg acgattagca
    38941 caaccacaca acaaagtaat gcttttaccg gatcattgaa gactgttact agaaatgcag
    39001 gtgctagtcc tagataaggt cctaaatatg gaattaggtt tgtcaatccc gcaatgaccc
    39061 caaataagaa agcataatcc acaccaatta ccatatatcc taaaaatgtg aaggtaccaa
    39121 cgaataaaca ttcgattgct tgtccgctaa tgtaattagc caatgtttta ttcagttgcc
    39181 ccaataactc cacaatttgg tttcttcttt tttctgggaa aaactgttgg atgtttggca
    39241 ctagtttttc tccgtctttc aacatataga acaagatgaa tggtgctgta atgattacta
    39301 cggttgccga agcgatcgtt ccaacgatcg aacctaaact attggatact ccactaagga
    39361 attgctgaat gattgtcccg taagaaatgt ctaattgctc aaaatattta ttgatatcta
    39421 cttccttgaa caaaggcaat tcggctacct cttttaacca gctttctacc tgtttgatga
    39481 aatcaggcat attagatgcc aatgaggaaa tttgctgtac tagacttggg atgacactaa
    39541 gtaaaatcca aataattgct gcaactaaaa ggagcagcac aattgcgaca gcccatattc
    39601 ttttgatttt tgctttcatc aacagcttga caataggatt caataaatag taaagaaagc
    39661 ctgcaatcag tacaggtgca aataacgtgg aaaaaaatgt cccgatcggt tcgaaaataa
    39721 agtttatttc tgatgaaatc atgatcaatg cagctatgat caataattca agcgaccaaa
    39781 acattagttt agactgtcta agtttttcaa acaatagatt tcctcctttc tttcttacta
    39841 gtatatatta tattcgttaa aaattctttt attctttttt tatttttgaa aacttgccat
    39901 actttttatt tgttttattt tccagtatac tatacgaaag gaaggtggct agaccatgaa
    39961 agttgtacaa acaaaagata caatgagcaa tatttactta gatgctgtac ggatcaggca
    40021 tcaagttttt gtagtcgaac agggtgtgcc tttgtctcga gaaatagaca aagatgaagc
    40081 ccattgtatc cacttcgtcc tgtactcaga taaaaaagaa ccgcaaggaa ctgtacgtct
    40141 tttgccttta gaaaacggaa aaatgaaatt acagcggatg gcgattcttt ctgaatacag
    40201 acaccaaggg ttaggaaaaa tattgattga agaagcagaa aactttgcta agaatcaagg
    40261 ctataatacg attttattag gggcacagtc cacagctgaa actttttacg aaaaactagg
    40321 atatacagct tacggcgatc cttttgaaga tgctggcatg cctcatattt atatgaaaaa
    40381 gactttataa aaatttattt cgaaaaaagc aaaaaacagc aagcttttga gaaaaatctc
    40441 tcactggctt actgttcttt tttgtgttta cttacgcgac aattgcttgt aacggttata
    40501 ccaaatatca atatagcctt ccgagaacgg gccatctcca ttattgatcc agtcaaccag
    40561 aatcttcaca ttttctttta ggatgaagtc aatatcgttc gggtaattca tcattttgct
    40621 gtgcatctca tattcatcga catccaatag tcgtttctcg ccgtcaggaa ataccttgat
    40681 atccaagtca tagtcgatat atttcaaggc ctcatcgtcc agcaaaaaag gtgaagctag
    40741 attacaataa taggaaaccc ccttttctct gatcattgct attacattga accagtattt
    40801 tttatgaaaa taaacaatcg ctggttcgcg cgttacccat cggcgtccat cagattcagt
    40861 gactaatgtg tgatcattca cgccgatcat agagtgttcg cttgttttta ataccatggt
    40921 atctcgccac gttcgatgca atttgccgtc gtgcttgtaa ctttggatcg tcacaaactc
    40981 tccttctttt ggaactccca tagcaaccct tccttaagca gcattttgtg ctttgtgttt
    41041 gggtgttcat tacaccgaca tcataattat tatatcaata aaaatgagct ttgcctagaa
    41101 aggattccta ttgtttcgat ttttctctaa ctgatctacc agtttcattt ggaccttagg
    41161 aaaaacgacc gaatcaaatg catcaggctt caaccacttc gaagttttgt tctcggtaaa
    41221 ttcttgttct gctccctcgg ttgcccgtcc gtaaaaaagg agaacatgcc atttcagatg
    41281 actaaaaata tgagtaactt ctcctaaatg tcgtgtctgc caaacaaaga gttcctgctt
    41341 ttcaaatggg agattttgct cttcttctgc tactaggtca tcaaacagat cgatttctgg
    41401 ctttgtttcc cattcttttt tcaatcgctc atactcctct tgtgtcactt ccatcattgg
    41461 aaatgtccac atattcgcca gcaatttttg gctatctctt tcttcaaaat aatacgctcc
    41521 agaatgattt tgaagagctg cactgatata ataaacgtct tttggctttg ctttttttgt
    41581 ttttacagga aaactcgttt ggattcctcg tttgttagct aaacaaaagg cttggatcgg
    41641 acaagtctca cactttggag aagttggtgt acagatagct gaacctagat ccatcattgc
    41701 ttggttgaat tcacctggat gcttttcatc aatgattttc cgcatcgctt catcaaagat
    41761 tttcctgcta gaagcttttg cgatgtctgc ttcaatgcaa aacaatctgc tgactactcg
    41821 catcacgttg ccgtcaactg caggttctgg aagtccaaat gcgatacttg caatcgcgcc
    41881 tgtcgtatat ggtcctattc ccttcaatga gctaatttct tcaggcgttt gcggcatttc
    41941 cccatcaaat tctgacatga tctgtttagc agcagcttgg atgttccggg ctcttgagta
    42001 ataaccaagg ccctcccatg cttttaaaag cttttcttct ggagcatttg ctagctcttc
    42061 aattgtagga aaccattcca tgaaacgata aaaataatcg atcactgtgt ctactctggt
    42121 ttgctgaagc atgatctcag agatccagat acgataagga tcacgattat agcgccaagg
    42181 caggtttctt ttttcttgtt cataccattg aatgaactga tcttgaaatt cttttgtttc
    42241 tttatctgtc catttttccc attctttatt cactttcctc ttccttttcc gtaatgaatc
    42301 gctggatcat atccaaatca aatccttttt gatataagct ttgctttact tttagatttc
    42361 tttttttagg ttcaaacctt aaatttcttc gccatagttt atctccttgt ttgactaaat
    42421 aatcatattc tatttcttga tctgcttctt gcggaagatc agctatcgct tgttggatca
    42481 cttcttgaga aaagcctttc gtcatcaatg tttgttggat ttttctcaat aattcttttt
    42541 ggctttttcc gtgatttttt ccaaacgctt tttcggctgt ctgatgtgca ttttgaactt
    42601 ggtcttcaaa tgtatattgg ctgagcgctt gttcaatgat ttcaggtttc aaaccttttt
    42661 gctgcatctg ctgcgctaat ttctttggtc ctttgtcact taagcgcata ttcgtgcgca
    42721 cataactttc cccatagacc aaatcatcga caaggtccaa ttcttttaag cgggctacga
    42781 ttttatttcg atcttcttga aggatttcct tttcttttaa gtatgttcgg atttcttttt
    42841 ccgatcgcaa ctgataactt aagtaattca ttgctaggtg aaaaccgaaa tcagctccgc
    42901 tactcttctt gatctcttgg atagtttctt cgtctaattc ccgtcctttt aataatcgat
    42961 agcgtactaa tacatcttca gatacttgta aagcttctcc ttcagataaa tctacgcgat
    43021 agaaagggcc tttgccttta ctgattctcg taacggttaa cataaacatc ctcctcgaac
    43081 atatattctc tatcatacca cagaaaatcc ccgtaatcag gtattatatg ttagaataaa
    43141 ccacagaaaa aagaaaaagg gagatactta tggcagaaac aattgtcaaa actggacaaa
    43201 cggttccttt gaaaatcaag cgtttaggca taaatggtga aggaatcggt tatttcaaac
    43261 ggttgatcat atttgttcca tatgcactac caaaagaaga agtactagtg aaaatcacga
    43321 aagcaactcc tcgctatgca gaaggagaat taataaaagt caaaaaaaca agcaaagatc
    43381 gcgtagtggc accatgtcct gtttactatg aatgcggtgg ctgtcaatta caacacttag
    43441 cttatcaagc tcaattggat tttaaaaagg atctgctttt gcaagcattg gaaaaattca
    43501 aaccagcagg ctttcgatct tatcaacttt tgccgacgat cgggatggat gagccttggc
    43561 attatagaaa caaagcacaa tttcagttac gaaaaaataa gcagactcaa aaaattgaag
    43621 ctggtttata cgaagcaaac tctcatcagt tagtccctat aacagactgc ttggttcaag
    43681 aacctcgaac acaaaaagta atgaatactg ttgttcaact attgaataaa tttgatgcac
    43741 ccatctatga tgaacggaca aacagtggaa tcttccgcac aatcatggtt agaatcggtg
    43801 ttaaaactgg ggagttacaa gttgttttta ttactcgcag taaaaaattc ccgcaaaaaa
    43861 acgcaatgat ccgtgagatc aatgaacgac tgcccgaagt tgtttcaatc atgcaaaatg
    43921 tccaggcaca aaaaacgtct ctcgtgatgg gggatgaaac gatccactta tggggaaaag
    43981 aagccattga agaacaaatc aatgaagtga ctttcgatct ttctcctcgt gccttctttc
    44041 aactcaatcc tcagcagacc gaattgcttt atggcgaagg aatcaaagca ttagatgtcc
    44101 agcctaatga aacgatcgtg gatgcttatt gcggagttgg gacgatcggc ttaagtgtag
    44161 caaaacaagc caaagaagta cggggaatgg ataccgttcc tcaagcaatc gctgatgcaa
    44221 aaaaaaatgc ggaaagatta ggatatcaca acactcgata tgaagtcggg acagcagagc
    44281 aagttctgcc taaatggttg aatgaaggtt ttcagcctga tggtatcatc gtagatccac
    44341 cacgtacggg tttggataac gcattgatcc aatcattatt gaaataccct ccaaaaaaac
    44401 ttgtttatat ctcctgtaat gtttctacac ttgccagaga tctggttaat ttaagtaaaa
    44461 agttcaagat tgaatattta cagtccgtcg acatgtttcc tcagactgca cgttgtgaag
    44521 ttgtcgtgaa gatgacgaga ctcgctgact aaatgaaaga ggttgtgaaa aagtctgccc
    44581 tgtatcaagt aataagaaca gccaaacaaa aatagctctt catgttttat gaatactgta
    44641 cttgtgaccg aagagttaac aggaggacac tatttattta aaaaaagggt tgtgacatag
    44701 tttttgtcac agcccttttt gattattatt ggatattcac gtctatcact tgataaaatg
    44761 catttgtcgt atcgctgata ttccatactg ctaaaatcac attatatcct tttcgatcct
    44821 gcggaagtgt gatcgtttgg ttgactgttt ttggtggaac agttgctttg tcgtctattt
    44881 ttgtgatcaa ttcaaaatca ctaaatttca aaggttgatt aggattccaa gttggtttgg
    44941 taatgtagta atcccaagta cttgtccgat gttgtgctgt taaattccaa gtaatattta
    45001 atgttcctgg attaaccatg tttttatgcc atctgttaga tgtctgttca tctaaaggct
    45061 caaagccgct gactcctgca ctagcaattt ttccatcaat gaatgtattt ttggtagctt
    45121 cgatactttg cggttcccat tgtgctcttc cgacattttg atttaagttt cctgctgctg
    45181 atgttcctag gtatgcacgg cttcctggag aagtaatata accatgcgca aaacctggaa
    45241 tgctaaaacc gaacaaccct aaactaacta acccaataaa caataacgta ctaatctttt
    45301 tcatgtagaa acctccttca tttgataacc acattgtaaa cgctttattg tattaacaca
    45361 aacattatgc ttttataatt ttatgttgaa gtaaaaaagg tgaaaaaagt aagatgcaga
    45421 ttataacaga agtagacgag aaaagtacaa atagaaaaca tgagaatcaa atcatatgat
    45481 ctttttcaaa aacatcattc atataaacaa atgttcaact ttttaataac aacaattatc
    45541 aatttttttc ttacttcaca aactaaatac aggtcttgct gtgagccttc atggtaaact
    45601 aatattatag aaaggagtta gtaaaatgaa gatgataaaa gatattcgtt tttgggtttg
    45661 tgttatagga attgtcatat taggcttttt atcagggttg cttagcggaa atcccggaaa
    45721 atattactat tctttgcaac ttccaccatt tgcaccacct agctggatat ttgggccgat
    45781 gtggacctta ctgtatatat tgatgggtat ttctctttat ttgttactta atcacaacaa
    45841 caaaaaacag cgaaacaatc ttgtcggtct ttttgtgatt cagttcattt ttaattttat
    45901 atggtctgct ctatttttta atttaagaaa tattttcatt gcagcaatcg atattacact
    45961 tttagttatt ttcttgtcag tgcttatgta tcagttgtgg ctgcatcatc gcctggcaat
    46021 gtggctcatg attccttatt atttatgggt actattcgca actctattga attattcaat
    46081 ttatttttta aactaagcgc taaaaagaga cgtcctgtaa tttaacacat agaaaaagtg
    46141 ccacctaagt tagatttttt gtctaattta ggtggcactc tttattaacc aaactatttt
    46201 tttctagtac ccacttgttg ttccatactc attattctgg tcataaccat aaccattttg
    46261 ttcctgtcca gcgtaaggat cataactttg agtatcttga cttggtgtat agtactgact
    46321 tgtatcttct gtttgctgat aataggtatt cggatcttgg tactgatcat atgtgttttc
    46381 tgaataggaa gaataatcgg tgtacccttc actattcgat ccatcaccgt ttgtcgaatc
    46441 gccatcttcc gaatcatcaa agaacatcat tgaagcattc tcatttttca aattaggtaa
    46501 tgtatcgctt gtttttatat ttaattgttt cttcagttca ttttggatcg aaagaagatc
    46561 attttttcct aaaatttgat agtagatatc attgatcatt tgtccttcgc cttgcaactg
    46621 gtcttgtttg attgtttcaa acgccggggt gtagttcgtc gctacatcaa gcatgtcatc
    46681 ccaatctaag tccgtcgtca cattgttctc aactgctttt aaaatttttc tataattgct
    46741 tacgccatct aaacttaaaa ctttattgac gattttcgtc actacttccc gctgacgacg
    46801 ttgtcgaccg atatcgcctt ctggatcttc gtgtcgcatt cgagaatagg ctaaaccttt
    46861 ttctccattc aatgtttgtt cacctactgg tacatggaca ccatccaaag taaagtcaat
    46921 tttattattc actttgatgc caccgaccgc attgatcaaa tcttccatgc catccaaatt
    46981 gatcgttaca taatgatcaa taggaatatc gagcagttgc tcaattgaat ccatggccat
    47041 ttcgacacca ccaaatgcgt aggcatgatt taatttatct accgttccat aacctacgat
    47101 attcgtataa atatctcggt ccaaactgat gatcgttgat tttttttgtt tcggattaac
    47161 tgttactacc atcatcgtat cagaacgtcc ttgttccgtt cttcctaatc caccggtatc
    47221 taaacctaat agcaaaaccg aaaaaggttc tttgtcatca atgcttactc tctcagaacg
    47281 tttggaggat attcgattag agtttttaga aattttgttt actgtttggc tcgcttctcc
    47341 cataaatctg attccgtatg ctgataatcc ggcaatcagc aaaacgaata cccctaacaa
    47401 ggttaataga actttttgcc atcgcttcat aatacacctc tgtttcacat tttctaaaat
    47461 attgtctcat ataactgttc aatcagcaaa ttcacttttt aatcataaga aaactttaaa
    47521 aaacattttt gtgtaacgat tattattttt taaaatttct caaaattctt aactatcata
    47581 ccataccgtg aaaaagatcg aaagaccgaa tacacgcagt tcgcaatcgt tttatctttt
    47641 tttatgtatt ttttccagaa aaagcgaaca acaaggtatt ttccaaataa tcgtgcagta
    47701 aaatgatcgc acataccttt cccatctttt taacagtctt tgcctaccgt tcagattatc
    47761 attcgatttc acgacaattt cttcctatga ctggtccttt tgttaaaccc atcttctatt
    47821 cgttttattt ccagctcact gcatggctct taaaatgact cgaactagtg attgcattac
    47881 catcatcttt taatggatag atatgctcct cttcaaagta aaacgatttt cttttaaagt
    47941 aatagaattg acatggtaat gagtatgtat actagtggtt tcacaaatgg tcttttattc
    48001 aacatccaaa aactgaaatt tctttaaaat ccattactaa cgaagaaaat gttccttgct
    48061 tgataattag ttaggacatg tcaagtgaac aaatgcattt acttagccaa aaaatctaaa
    48121 aacttcataa atagacaaaa agacacgact ctctaagaag tcgtgtcttt attcaacaaa
    48181 ttattataat ttagtcactt cagcagcttg aggtccgcgt gcaccttcaa caatcgtgaa
    48241 ttctacttct tgaccttctt ctaggctctt gaagccgtct ccttgaattg ctgagaaatg
    48301 aacaaatacg tcatctccgc cgtctactgt aataaaccca aatccttttt cattgttgaa
    48361 ccatttaacc gttccgtgtt ccataaatgt atatcctcca aaaaagttct tgcaagtaac
    48421 cttgcaagaa cattataccc aatttagaaa acgctagcaa gctttcgctc ttttattttc
    48481 caaattattt ttaagactga tatgacggag gaaatggtta ttttgctgct tcgaaacgtt
    48541 tgtttacttc atcccaatta ataacattcc agaatgctgc aatataatct ggtcggacat
    48601 ttttatattt caaataataa gcatgttccc aaacgtccaa acctagaatt ggtgtctttc
    48661 cttccatcaa tggagaatct tgatttgcag tagaggtaat agctaatttt ccattttcca
    48721 ttacaagcca agcccatcca gaaccaaatc gtccagcggc tgcttttttg aattcttctt
    48781 taaaagaaga aaaatcacca aaagcttcat taatcgcttc ttttatttct cctgtaggtt
    48841 cgccaccagc atttggtgcc atgatttccc agaaaaatga atggttagca tgtccgccac
    48901 cattgttacg tacagctgtc ttgatatctg ttggaatagc gtccatatca gataataatt
    48961 cttctattgt tttttcgcct aattctgggt atttctcaat tgctgaattt aaattcgtca
    49021 cataagtatt gtgatgctta tcatgatgca gatgcatcgt ttcttcgtca atataaggtt
    49081 ccaatgcgtc atatgcataa ggtagatctg gtaatgtgta agtcataaaa aaattcctcc
    49141 tcaatgtttt cttaataatg tctacattac aaagagtaac aaagagaagg aaattcttca
    49201 attgttatgc ctagtcgtct ttttcacttt gacctacttc ttcttcaaaa acctcagttt
    49261 ctttaaaaat aaacaacttg ctgaaaatat agttcgcaag gataacaata atttgagtga
    49321 tgattttcgc cacatactca ttgctgttca taacattgat caacaatacc attgccccca
    49381 tgtccaatcc gtaagaaagg atacggtaga agaagaactt accgcattca ataagtgcat
    49441 gtgtaaaatt cggtgatttt gaatggaata cccaaagttt gttcgttaca aaagcaaaca
    49501 atacagaaca aaaccacgaa atagaattgg ataaggccat acttaaatca aaatattggc
    49561 gtgcgagagc aaaactaaca aaatttacaa tcgtagccaa accaccaaaa aagagataga
    49621 ctagtatctc ccaatatcct tttgcttcta aatatccttt taactgtcga tactgcttca
    49681 tgagccaacc taccttatta aaaattctta tttatcatac tctatatttc aataactttc
    49741 atctttattt aaaaaaatta attttttttg atcctttgcc tttgtctctg cttggatcgt
    49801 cacatgtgta ataccatatt ttaattttaa taaagaaatt atacgaggat atatcttttc
    49861 aatctcgctt agtagctgat cttcaaaatc aatatgtccg tcgaaaataa ttatcttttc
    49921 gctatactgc caaatatgga catgatgaac atcctttacc ccatcgattg cttcaatttc
    49981 ttgttccatt ttttcatagt cgatagcagg tgcactttgt atcaaaatag cggcagcttt
    50041 tttgatcacc attatcgctt ctatcaatat atatatcgaa atgatcagtg taataactgg
    50101 atcgatccag tagatatcga aaaaacggat caaaattgct ccgataatca cacttaccga
    50161 agacagtgta tcacttaaca aatgcaaata actagatttt atattcaaac tttcatctgc
    50221 ttcttgtcgt aataataata ctgagaacaa attcgcgagc aatccaataa ctgctactgt
    50281 tagcatcaaa tgactattta tcttttctgg tgaagtgaac cgcttgaatg cttcaacggt
    50341 taaaaacaag gataatatga tcaatatgaa tgagttgatg aaagcactca atatttctaa
    50401 acgctggtag ccatacgtac gttttttatt tgcttctttt cgggccattt tttgtgcccc
    50461 gtaagaaaaa ataattgcca atgtatcact aaggttatgg aaagcatctg aaacaagaga
    50521 caaactccct gacataatcc ctccaataaa ttctgataaa gtgatcaccg cattcaaaac
    50581 cactactcca aatagttttt ttgatgatct ctgtggctgt gtaggcacta ttccttcctc
    50641 cattttattt atattggata gtctttagac aagaataaca tttttttgat atttcaccaa
    50701 tcattcagct gtttccttaa agcacttgta tttcacttga tacttcgcta gaattgcaag
    50761 tgatgacact actagatgag gagtacttat gacaacaaaa caattaatta tcagttcctt
    50821 tatacgattc aaagacctaa aggaggcgag aaatgtccca ttttggaaag ccattgtcta
    50881 tcttttagtt ttgagtataa ttatggcttt gccaatctct tttcaagttt ttcaagtctt
    50941 aaacaacatt caattggatg ggcaaaagat tgctactcgc attcctgatt ttacaatcca
    51001 aaacgggcag cttcaaaccg aagaaaaaga aggattcatt taccaaacaa actccattat
    51061 cttcacattt gatccagagg gaaaacgaac agaacaggac atctctactg atttgatggg
    51121 gaattttgtt agtgtcggga tgttaaaaaa caaacttatc atagctcttc ctaatacagg
    51181 cacgacttct gctttgttga acaataacca attagaactt ccctacacca atgaatcttt
    51241 aaaaaactta acaggtaagc agctccgttc ttttttaagt gaagcatcta taccactttg
    51301 gataaaagtc ctgacattta tattttctat ttatcctagt tttctgaatc tgctgatcac
    51361 tttgctattt accaacattg ctgcttttct ctatgcacgc tttcgactaa caaaagctac
    51421 ttttcttgat tgtttaaaaa cattgatcta ttcaatttca ttgcctacta taattgcaac
    51481 aatcttgatg gtctttcttc cttcttttga cactagcgcc ttcattgctt ttgcaggaat
    51541 ctttattttt gctcaagctg tcaaaggatg ggcaaaaata tcgatcaatt aagaaataaa
    51601 gacaactcgt tatttctact tcaatagacc cttttagagg ttgtgacatt tttttgtcac
    51661 agcccctttt ttatttgaaa aatatttctt tctcaaaaaa caagcaaggc gcttcaaagt
    51721 tcttcttgta tgcgctacac ttagagtaaa gaaaggcggg aaattaacta tgtcagaaag
    51781 aatcatttat gcagatcgtc catttcgtga tgaatttatc gaagaaatcc atactatagc
    51841 gcctggttat atctttaaaa cagaattaca accagaggat ctacaaaatg tagagatcag
    51901 tttgggctgg aactcagact accaagaaaa actattagcc tcttctacct tgaaatgggt
    51961 ccaatctatc tcagctggtg ttgatcagct accccttgag actttttcaa agaaacatat
    52021 tttactttca aatggtagtg gcatccacag tgaatcgatt acggagcatg tcttaggaat
    52081 catccttggg tattctcgtg gtctttttca agcacaaaag gcacagagtg acatgaaatg
    52141 gttaggttca tcggtccatt accaatctat ccaagggaaa aaaatactaa tcattggaac
    52201 tggccatatt ggaaaaatga ttgcaaaaaa atgcaaagca ttagacatga actgctatgg
    52261 aatcaataca tccggtcatc cagcagaagg aatggaaaag actttttctt taaatcaact
    52321 aaaggaagtt cttcctgaag tagatttcgt agtcaatatc ttacctctaa cggaggaaac
    52381 aactgggctc tttgatcagc atatgtttga acactttgat ccaaaatctg tattcatcaa
    52441 tgttggccgt ggtgcttctg ttaagacaca agatctggtg caggctttaa ataaccagca
    52501 actttccttt gcagcattag acgtttttga ggaagagcct cttccacaag atcatcctct
    52561 ttggaaaatg gacaatgtac tgattacttc ccacattgca ggtttgacac ctgattttca
    52621 aaagaaatta atggctattt ttttaaccaa tctgaaaagc tactttgcca cccatgagct
    52681 tcaaaccaat caagtaaaat tgtctgcagg atattaatag ctgaaaacaa tattttcaaa
    52741 agaaaaaaga gaaggtgaca aaaattgtgt caccctctct ttttttattg ttttttaaag
    52801 tgataggttg gctcctccag gttgggatta taatcaacaa gtaaatcata tcctttaaag
    52861 aaccattcat ctgcttcctc gataaaaaaa agtgtcccat caatttccac tttctttaca
    52921 ggatgttctg gctgttcaac tgacattcca atcgaaaaac catcatgaac ctctgttttg
    52981 ccgtagactt ttccaaaaaa acgtattccg taagtttctg gcagatcaat ttctgagtca
    53041 aaccactttt gtgcttttgg cgttaccgtt aatttcatcc caacaaccta ctttctcttt
    53101 tctttctatt gattcatctt atcgtctaga atatccacat cgcctgtctc tccaataacc
    53161 agtagattat cattttcacg aacatattca tctgctgcag gagaagcgat aaccgtttga
    53221 tctactcttc gtatggcgac tactgttaag tcaaaacgtt gcctgaaatt taattcagct
    53281 aatgttttgt tgaaaaattt aggattggag actcggattt ctgctaaaga atatttatct
    53341 gacaactcta ggtaatctaa aatattcttt gacactaaat tatgcgcaat ccttattccc
    53401 atgtctcgct cgggatggac gacgcgatca gctccgatct tttcaagtac tctggcatga
    53461 tactcatttt gggcttttgc taaaatattt ggtacgccca tttcctttac catcagagta
    53521 actaagatac tggcttgaat atcttctcca atagcgacaa ttacatgatc gaaatttctt
    53581 atgcctaatg atcgaagtgt catttcatcc tgtgcatttg ccacaacagc atgagtggct
    53641 atattcatat attcattgac ccgatcttca ttactatcaa tagccagtac ttcttgacca
    53701 gcttcgatca gtgttcggca gatacttccg ccaaatcgtc caagacctac aattgcaaaa
    53761 ttttgtttca ccgttttcct cctttagtat cttcttttct cagtataaca aagcctttac
    53821 aaaaacaaaa gcaagcatca ttattgtaaa aaagccaaga aaaataatag ttattttctc
    53881 agcttttgat tatcctcttt atcatcgttt ttatttcacc aaaccatgtt tgaatgcata
    53941 gatagcggct tgtgtacggt cttccacttc tagcttagcc aatatattgg aaacatgtgt
    54001 ttttactgtt ttcagtgtaa taaacaattc gtccgctatt tcctgattac tttttccttc
    54061 agaaatcagc attaaaattt cattttcccg attcgttagt tcttcatgta atacaggctc
    54121 atttcgccga ctcatttttt ccatcatttt tgttgtcact tctggttcta atactcgctc
    54181 gccacgttgc gttgccctta tagcatcagc aatttcatgt gcggtagagg tcttcaacaa
    54241 ataccctgct gcacctgctt caatggcagg ataaactttt tcgtcatcaa taaaactggt
    54301 tacgataatg attttagctt ctggccaatc ttttaagatt gcttttgttg attcgatacc
    54361 atccatttct tccatgacta agtccattaa aatgacgtcc ggtcgaagtg tcatcgcctt
    54421 ctcatagcct tgtcgtccat tttcagcttc tccaattact tcaatgtctt cttgaataga
    54481 taaataaaaa gagacgccta agcgcaccat ttcatggtca tctactaata aaacttttat
    54541 catccttatc tatgcctcct tcatcaaagg gaccttgatt tctacacttg taccttgacc
    54601 tttaaaacta atgattttta ctgttccacc aataccagct actcgttctt tgatattgtt
    54661 caatccataa cttcccgttt tcgtttcatt catattaaac cctgttccgt cgtcaataat
    54721 acgtaacaaa agattattgt ctattttgtg caaatatacc tctaattcat tggctttagc
    54781 atgtcttaat gtatttgata acaattcttg aacgattcgg aacaagtgat cctcaatgga
    54841 actagatagt tttacatctt ctacatccca tttcagcgaa atctggattt tattttgcaa
    54901 ttccttcaat aactgctcga taccttgctt taaacttttc tcttctaaat tgactggacg
    54961 tagatgaaga agcagcgcac gcatctcaga ctgggaagcg ttgatgatct ctgctaccat
    55021 tttcaactgc ttgcgaaaca tttctggtgt ctcgcttttt tctgcttgct ccgtcaatgc
    55081 cgacatcatc atcattgctg caaacaattg ttgcgaaact gaatcatgca gctcacgggc
    55141 taatcgatgt ctttctaatt ctaaaatttc ttctttggtt tgtccatcta catattgggg
    55201 acggctcgtt acaatttgta attcactaga tacttcaatc attttttctt ttatcttagt
    55261 gatttcctta tcaatatctt tgatgtataa atcgtccgat gcacttggga tcggaagaag
    55321 caacaatttg gattcataat tgccagaaga aagtgcacgc agcttttctt cgattttccc
    55381 gtattttgtc ttttgaataa gtgataataa tagaaagaca attaatccta cacctaagga
    55441 aatggctaac aaatggaaga taagcggaac atataataaa cgtgcacgca gtaattctcc
    55501 ccaccaatga ctttgattgc tggcataaaa ataagtgaaa agtgaaaata gaataataag
    55561 gaaagccgca atccccgaat aaacagctag catcgctctg gatatttttc ccatcatatt
    55621 cgtatcacct cgacatctcc aatcaaggta ttagtaataa tcttcaatct acgagggttt
    55681 tcatcataat cactgctgta gattttgatt tgttcatttt tcaaagaaaa ttgctcttct
    55741 tcaaacaaga catttcctac taatgtcgca tgctctaatc ggatggctac gcccaaaggt
    55801 actaagatcc gtgttctgcc tatccctttt cttacaatca caatattatc atcttttggc
    55861 aaaagcgtat tgcccaaatc tatgatagta tcaccagaaa tgacggcgat gttgatatcg
    55921 tcccattcat agacgtcgtt tccaatacgt tggttaccga ataattgctg tcttttccgc
    55981 tcattattat gtgtttttaa ttgctctgtt tggatcatca tgatctgttt ctttcgccaa
    56041 aaagcatttt tggttaaatc tatccctgaa atctctacac ctttcagacc aataaataaa
    56101 acggcaaata cagccatcat ccataaagca ggactgttga ctaagctaaa aaaaatgacc
    56161 aaacttccta atatcaattg aaaattttga aatttagtac gaggatgttt gcgcatagct
    56221 agatatatat tgaaaatacc gaatatcact aaaagcaaaa gtccagtatt gtttacgatt
    56281 tgccaaacag caaataacag cagtagtgct tcaactacca caaaaaaacg ccaagaacta
    56341 ttcatcgctc tttctccctc ctgacacatt ctcatttcat catacctatt tttttaactg
    56401 ttggctatag gactttagat gtaagatcgt ttgttgaaat ctaaaataca caaagagaaa
    56461 aagatctaga gtgtatcatc agtaagatga actctctaga tctttcagtc agactacgct
    56521 tgttctactt tgacgatttt aactaacata tcgccacctg gagtagcaat agttacttca
    56581 tcatttttac gtttgccaat caatgcttgc gcaataggag aatcattgga aattttgcca
    56641 gaaaacggat ctgcttcagc gcttccgaca atcgtgtact cttcttcttc tccatctggt
    56701 agttcgatga atgtaactgt tttaccaatt gatacctcat ctgcatctac accgtcgtta
    56761 tcgatgattt gagcaaaacg aatcatattt tcaattgttg tgattcttcc ttcaacaaat
    56821 gcttgttcat ctttcgctga ttcatattca gagttttcgg aaagatctcc gaaaccgcgg
    56881 gcaattttga ttcgttcaat gatttctccg cgtttgactg tttttagttc ttctaattct
    56941 ttttctaatt tttctttacc ttctagagtc attggataaa ctttctctac catgtgtaac
    57001 gtctcctttt ttcgataaaa taccgcaaaa ggtaatcttt tgaggaatat agtccaaaaa
    57061 agattaccac gcttttcttc aaatgtaaat gtttttctaa aaatgatcaa tgtctagagc
    57121 ctgtttttct aagaattatt tacatatttt tccactaatt ccatatgttc ttcataagtt
    57181 ttagcgaaat aaacatttcc agtgctcgta tctgctacga aataataata gtcattttct
    57241 gctggttcta atactgcctt gattgcctct tcgcttggat tgtcaaaagg tccaggtcca
    57301 tagccggtat tggtatacaa attatatggt gaatctacag ctgtatcttc atacgtcact
    57361 aattctttgt gttcacctaa tgcgtataaa acagagatat ctgactgaag tggcatattt
    57421 gctttgattc ggttaaagaa gacttgtgca atgtttttac gatcattttc ttttacgcct
    57481 tctttttcaa ctagagaagc tagagttaaa acttcttgta cagtcaaatt tttctgcttg
    57541 atagctgaat aatagttttg catgaccgta ttcgtcttgt tcaccatttc aatcactaga
    57601 tccttaagcg aagtactcgt gtaataatca tatgtggctg ggaataagta accttccaaa
    57661 cggtattttg tgttctcagc tttagatgca ctttccaaca gttctgggaa tttttggtgc
    57721 aactcgttaa agaaagtctc atcattcact aaatctaaaa attctttctt gtctttacca
    57781 gttaccttcg ctacccgttc agcaatctga tcgatatcgt acccttctgg aatggcaatt
    57841 ttcccatctg caacttttgt tggctcagaa gtaccgcctt cttgcaactg tttgccaatc
    57901 tcgtcaagag tcatattagg cgctaactga taatagccag cttgaaagcc agttaaattt
    57961 ttaaattttg tataataatt gaaaacaatc ccgcttttaa taatattgtc cttctccaaa
    58021 atctcaccaa tctgcttgtt agaagagcca ctcgggatct cgacttggac taattggtca
    58081 tctgatttat ctaaaggctt caacccgcta tctacataac gatacacggt aaatccaaga
    58141 aatccgccaa taatcaataa tgtaagagca atcactaaaa tgatcttcct cacaatctta
    58201 tcttcttttt tacgcagatt tctccgcttg tcgattatat ctgctgtagc tgccttactg
    58261 gcttcttttt tatctttact tgggtgttgt ttgtttcctg acaaaacaac tcctccttac
    58321 tatcaagttc gtaactttat tcattataca tgatatttga attggcgtga atgattttat
    58381 ttaaatattc tatcaaaaat gtaagtagta aatccattac tataagatta aaataaatgt
    58441 tatcaagata caagctattt tagctttatt ctttctgtaa aaaagaaagg agactaaata
    58501 aactattatg aaaaaaggaa aacatgctaa aaaaagaaaa tattcatttt taatgatcgt
    58561 tgtcgtgttg tttagcctta gtgcgttctc tacaggaact attcttggat ggcaatttta
    58621 caataatgaa cactctcaga tagttgccca tgctcaatca gctcaaaaaa agttgatact
    58681 gactaccaat gaacaacagc tcgttgaaaa aatcgatcaa ctattaagaa aaaatgatta
    58741 tattggctca atttacgtaa agaaaaataa tcgcatcatc atggaaaaag gctatggcta
    58801 tgcgaataaa ctcacgcaac aaccaaatga tccttctctt tattatcaga ttggttcggt
    58861 tcaaaaagca ttgactactc ttttgatttt aaaacaagtc gagcgtgggc acctttcttt
    58921 agatactaag ctggctcaat tctatccaca aattgctggc agccagcaaa ttactatccg
    58981 tgacttgctg tatatgcgct caggcttgca ccgaactgct tctccaacca taccgatgac
    59041 tgatgaagaa gtcgttcaat ttgcgattca gcacttaaaa tttacagatt atcagacctc
    59101 tcattatgat tcattgaact ttaccatatt gactggaatt ttgatcaaat taacaaatca
    59161 accgtatgaa aaattattga ccgatgatat catcgaacct ttaggattac agcatacaaa
    59221 attttatgac caagtcaaaa attcatccaa tcatgcacta tcctatcaaa tgtctaaaga
    59281 ggatgattac tttaaaccac tgacagaatc tgaaacagag atccataacg aattagggac
    59341 tggtaatatc agtatgtccg tctatgattt aaatacgctg tttacacaag ttttatctgg
    59401 aaaactagtt tccaaagact tactcttctc attatggcgt gaaaatgcga atggacatcc
    59461 atatagtggt ggggtatata gcggagataa ttatatcctg tcgcaaggaa atatcgactc
    59521 ttttgataca tctgctatat ttagcaaaga ttcacaggat gctatcatca tggaaagcaa
    59581 tataaaagct gacaaaacta tcaaactgcc tactgctgat ctaagaaacc aaatctatga
    59641 cctgatggaa ggaactacta agttggcaaa tagctgatat gatgaaaaga tagcaaatac
    59701 tacttctttg aatagcattt gcttaaaaaa agtactcgac taaacgtagg aaagagtgtg
    59761 ggacaaatct aaaaatcaat ttttgctaca gactttaatt ccaaataaac agaacagaaa
    59821 gaggtgttct tctttctgtt ctgtccaaat tattctaaga tttctcttta ccaacattat
    59881 ttgacaaagg gcctattaaa tctaaaatag tatctttaga ttgctaaaat atgtgctagc
    59941 aaatcattca tggcttgttc ttatttttaa ttttatgcaa taaaaaagaa gaacaattag
    60001 cgtgattata gaaataaata atgagatttt agaaaaaaat gtgtatgaat acctgattgt
    60061 tactttattt attccacttt cactttttac tgtaacaaaa ggacctcctt gttttagtac
    60121 tgtagcattt tttgaattaa cagtaaccgt taatccttta taccggaata caggtaaaac
    60181 tattgttttg ccttttgaag atttaaacgt aaatgtaatt tcatctttat ttggagaaaa
    60241 gctactataa ataacttgac catcttctgt aacttgatgg tttttcaaaa gcaattgatc
    60301 ttttttatag cctataggta cataatcaaa attctcaaat tcttcaagtt tattttccat
    60361 ttgt
//
LOCUS       DAYYGS010000005        58649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000005 DAYYGS010000000
VERSION     DAYYGS010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 58649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.005"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            144..1661
                     /locus_tag="ACHA44_000289"
     CDS             144..1661
                     /locus_tag="ACHA44_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HFM8903616.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1633..2922
                     /locus_tag="ACHA44_000290"
     CDS             1633..2922
                     /locus_tag="ACHA44_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8903617.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3097..3993
                     /locus_tag="ACHA44_000291"
     CDS             3097..3993
                     /locus_tag="ACHA44_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8903618.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGTIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            4007..4870
                     /locus_tag="ACHA44_000292"
     CDS             4007..4870
                     /locus_tag="ACHA44_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8903619.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4876..5439
                     /locus_tag="ACHA44_000293"
     CDS             4876..5439
                     /locus_tag="ACHA44_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8903620.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5461..6972
                     /locus_tag="ACHA44_000294"
     CDS             5461..6972
                     /locus_tag="ACHA44_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HFM8903621.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6984..7943
                     /locus_tag="ACHA44_000295"
     CDS             6984..7943
                     /locus_tag="ACHA44_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HFM8903622.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            8029..9108
                     /locus_tag="ACHA44_000296"
     CDS             8029..9108
                     /locus_tag="ACHA44_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8903623.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9360..10760)
                     /locus_tag="ACHA44_000297"
     CDS             complement(9360..10760)
                     /locus_tag="ACHA44_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HFM8903624.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10978..12639
                     /locus_tag="ACHA44_000298"
     CDS             10978..12639
                     /locus_tag="ACHA44_000298"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0005998 - xylulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFM8903625.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12596..13294
                     /locus_tag="ACHA44_000299"
     CDS             12596..13294
                     /locus_tag="ACHA44_000299"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8903626.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13310..14734
                     /gene="araA"
                     /locus_tag="ACHA44_000300"
     CDS             13310..14734
                     /gene="araA"
                     /locus_tag="ACHA44_000300"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFM8903627.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            15041..16200
                     /locus_tag="ACHA44_000301"
                     /pseudo
     CDS             15041..16200
                     /locus_tag="ACHA44_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(16177..16317)
                     /locus_tag="ACHA44_000302"
     CDS             complement(16177..16317)
                     /locus_tag="ACHA44_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HFM8903628.1"
                     /translation="MLAPSFLSIKECCFSYFSCTKFESKERKEELMKSKYSLSLWPFW
                     FL"
     gene            16318..18177
                     /locus_tag="ACHA44_000303"
     CDS             16318..18177
                     /locus_tag="ACHA44_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8903629.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18287..18721
                     /locus_tag="ACHA44_000304"
     CDS             18287..18721
                     /locus_tag="ACHA44_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903630.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18980..19141)
                     /locus_tag="ACHA44_000305"
     CDS             complement(18980..19141)
                     /locus_tag="ACHA44_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903631.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19160..20182)
                     /locus_tag="ACHA44_000306"
     CDS             complement(19160..20182)
                     /locus_tag="ACHA44_000306"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HFM8903632.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20310..20663
                     /locus_tag="ACHA44_000307"
     CDS             20310..20663
                     /locus_tag="ACHA44_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8903633.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20675..21841
                     /locus_tag="ACHA44_000308"
     CDS             20675..21841
                     /locus_tag="ACHA44_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8903634.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21933..22553)
                     /locus_tag="ACHA44_000309"
     CDS             complement(21933..22553)
                     /locus_tag="ACHA44_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8903635.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22745..23041)
                     /locus_tag="ACHA44_000310"
     CDS             complement(22745..23041)
                     /locus_tag="ACHA44_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8903636.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            23245..23895
                     /locus_tag="ACHA44_000311"
     CDS             23245..23895
                     /locus_tag="ACHA44_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HFM8903637.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            23911..25638
                     /locus_tag="ACHA44_000312"
     CDS             23911..25638
                     /locus_tag="ACHA44_000312"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903638.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            25635..27527
                     /locus_tag="ACHA44_000313"
     CDS             25635..27527
                     /locus_tag="ACHA44_000313"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903639.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            28231..29100
                     /locus_tag="ACHA44_000314"
     CDS             28231..29100
                     /locus_tag="ACHA44_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8903640.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            29309..30334
                     /locus_tag="ACHA44_000315"
     CDS             29309..30334
                     /locus_tag="ACHA44_000315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8903641.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            30373..31323
                     /gene="manA"
                     /locus_tag="ACHA44_000316"
     CDS             30373..31323
                     /gene="manA"
                     /locus_tag="ACHA44_000316"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HFM8903642.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            31417..33120
                     /locus_tag="ACHA44_000317"
     CDS             31417..33120
                     /locus_tag="ACHA44_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8903643.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            33122..35155
                     /locus_tag="ACHA44_000318"
     CDS             33122..35155
                     /locus_tag="ACHA44_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HFM8903644.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            35166..35606
                     /locus_tag="ACHA44_000319"
     CDS             35166..35606
                     /locus_tag="ACHA44_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8903645.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(35806..36933)
                     /locus_tag="ACHA44_000320"
     CDS             complement(35806..36933)
                     /locus_tag="ACHA44_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8903646.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(36936..37613)
                     /locus_tag="ACHA44_000321"
     CDS             complement(36936..37613)
                     /locus_tag="ACHA44_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8903647.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            37877..39643
                     /locus_tag="ACHA44_000322"
     CDS             37877..39643
                     /locus_tag="ACHA44_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HFM8903648.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            39658..40095
                     /locus_tag="ACHA44_000323"
     CDS             39658..40095
                     /locus_tag="ACHA44_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8903649.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            40108..41259
                     /locus_tag="ACHA44_000324"
     CDS             40108..41259
                     /locus_tag="ACHA44_000324"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFM8903650.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(41321..42019)
                     /locus_tag="ACHA44_000325"
     CDS             complement(41321..42019)
                     /locus_tag="ACHA44_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HFM8903651.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(42012..43364)
                     /locus_tag="ACHA44_000326"
     CDS             complement(42012..43364)
                     /locus_tag="ACHA44_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HFM8903652.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(43587..44987)
                     /locus_tag="ACHA44_000327"
     CDS             complement(43587..44987)
                     /locus_tag="ACHA44_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8903653.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            45148..45885
                     /locus_tag="ACHA44_000328"
     CDS             45148..45885
                     /locus_tag="ACHA44_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFM8903654.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            46010..46489
                     /locus_tag="ACHA44_000329"
     CDS             46010..46489
                     /locus_tag="ACHA44_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HFM8903655.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            46486..47253
                     /locus_tag="ACHA44_000330"
     CDS             46486..47253
                     /locus_tag="ACHA44_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6681 family protein"
                     /protein_id="HFM8903656.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            47483..48052
                     /gene="lepB"
                     /locus_tag="ACHA44_000331"
     CDS             47483..48052
                     /gene="lepB"
                     /locus_tag="ACHA44_000331"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFM8903657.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(48156..48758)
                     /locus_tag="ACHA44_000332"
     CDS             complement(48156..48758)
                     /locus_tag="ACHA44_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8903658.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            48864..49094
                     /locus_tag="ACHA44_000333"
     CDS             48864..49094
                     /locus_tag="ACHA44_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903659.1"
                     /translation="MPISFVKDREEKGKCVREILLDLPEWFGLPESTEKYIEESSKLP
                     LWCEKRKEEYLGFITLSQTSEDTAEIYSIVWE"
     gene            49085..49333
                     /locus_tag="ACHA44_000334"
     CDS             49085..49333
                     /locus_tag="ACHA44_000334"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903660.1"
                     /translation="MGIKKDYHHQGLGTKLFKEAEGYAAKHYKYLQVKTVDEGHYSIY
                     DQTICFYESLGFSRLEVFPNLWDEWNPCLVLVKKLEQK"
     gene            49439..50023
                     /locus_tag="ACHA44_000335"
     CDS             49439..50023
                     /locus_tag="ACHA44_000335"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8903661.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            50026..51099
                     /gene="prfA"
                     /locus_tag="ACHA44_000336"
     CDS             50026..51099
                     /gene="prfA"
                     /locus_tag="ACHA44_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFM8903662.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            51092..51931
                     /gene="prmC"
                     /locus_tag="ACHA44_000337"
     CDS             51092..51931
                     /gene="prmC"
                     /locus_tag="ACHA44_000337"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFM8903663.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            51924..52103
                     /locus_tag="ACHA44_000338"
     CDS             51924..52103
                     /locus_tag="ACHA44_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903664.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            52125..53150
                     /locus_tag="ACHA44_000339"
     CDS             52125..53150
                     /locus_tag="ACHA44_000339"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFM8903665.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            53325..54569
                     /gene="glyA"
                     /locus_tag="ACHA44_000340"
     CDS             53325..54569
                     /gene="glyA"
                     /locus_tag="ACHA44_000340"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFM8903666.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            54722..55669
                     /locus_tag="ACHA44_000341"
     CDS             54722..55669
                     /locus_tag="ACHA44_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFM8903667.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            55803..56432
                     /gene="upp"
                     /locus_tag="ACHA44_000342"
     CDS             55803..56432
                     /gene="upp"
                     /locus_tag="ACHA44_000342"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFM8903668.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(56571..56813)
                     /locus_tag="ACHA44_000343"
     CDS             complement(56571..56813)
                     /locus_tag="ACHA44_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903669.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            56934..57006
                     /locus_tag="ACHA44_000344"
     tRNA            56934..57006
                     /locus_tag="ACHA44_000344"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56967..56969,aa:Thr,seq:cgt)
     gene            complement(57125..57763)
                     /locus_tag="ACHA44_000345"
     CDS             complement(57125..57763)
                     /locus_tag="ACHA44_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HFM8903670.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(57886..58518)
                     /locus_tag="ACHA44_000346"
     CDS             complement(57886..58518)
                     /locus_tag="ACHA44_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8903671.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
BASE COUNT        19502 a        10242 c        12068 g        16837 t
ORIGIN      
        1 gttatattaa attatttgtt ttaatcatta ttaatatttt ggttaattaa aatcgataaa
       61 aaataagtaa gtttaacatt tatattattg acaacgcttt caacgaggtg tattattcat
      121 gtataaatta aaggaggtca gttatggaag ccacaatgtt gattagtagt gtcaagcgtg
      181 taggcaaagt tgatccgcgg ttgtacagtt catttattga acatatggga cgagcagtat
      241 atgaagggat ttatcagcca gatcatccat cagctaataa aagtggattt cgccaagacg
      301 tcattcactt ggtaaaagaa ttgaacattc ccatgattag atatcctggt ggaaattttg
      361 tgtcaggatt tcgttgggaa gacagtattg ggcccatagc aaatagacct agaaggttgg
      421 atttagcttg gcggacaatt gaaacgaatg aagttgggat tcatgaattt tataactggt
      481 gtcagcatgt gggggccgaa atcaatatgg ctgtcaatct gggtactcgt ggagtagatg
      541 cagctcgtaa tttagtcgaa tattgtaatt ttcctaaagg aacttattgg agtgatttaa
      601 ggcgttcgaa tggtcaagag agcccttttg gaattaagac atggtgttta gggaatgaaa
      661 tggacggtcc ttggcagatt ggacataaaa cagcggaaga atatggacgg ttagcagagg
      721 aaacggctaa ggcgatgaaa ctcgtagatg attcaattga ggtggtttta tgcggaagtt
      781 cgaatcggca aatgcctaca tttggtgatt gggagttaac agttcttgat caagcgtacg
      841 atcaaataga ttatttgtct ctgcatcaat attatggcaa ccaaaaaaat gatctttctc
      901 attatcttgc acgttcctta gacatggatc ggttcatttc tgaagtgaca gcgatgtgcg
      961 atacagtcaa ggcgaagaaa catagtaaga agcagatcaa cctgtccttt gatgaatgga
     1021 atatttggta tcattcagcg gaacaagatg agcaagcagc gccttggcag attgctcctc
     1081 ctttactaga agataactat aattttgaag atgcattact attaggctgt ctattaatca
     1141 ctttattgaa acatgcagat agagtaaaga ttgcttgtct tgctcaattg gtaaatgtta
     1201 ttgcaccaat caggacagaa acaaacggag aagcttggag acagacgatc tattatccat
     1261 ttatgcaagc tgctgtgtat ggacaaggag aggtgttaag cccacaaatt cgttcagcaa
     1321 actatgcgac ggaagatttt gaagaagtgc cgtatttaga aagtatagcc gtaataaatg
     1381 acaaagaact tgtcatattt gcagttaacc gtggagaaga agaaatgaat ctaactgttc
     1441 acttgtctga attatcatta gagggagtga ttgatttttc tgaaatgagt ggatttgata
     1501 caaaagcaac taatcttcct aaaagtgaac aagtaaaacc aagtcattca acaaatgtaa
     1561 aagctgaaga tgggaaaatc aattgtttat tgaaaccgct atcttggaat gtggtcagat
     1621 gtcgtttatc agatgaagga ggaacaaaaa atgaaaggta aaaaatggtt aattggctct
     1681 ttgttggtag ctgcaacagg tattttagga gcttgcggtg gcggaagttc agcagaatct
     1741 gattctgata aagaagtgac tttgactttt tggaatgggt ttactgcatc tgatggtgaa
     1801 atattgcaag aaatcgttaa tgaatttaat aaaacaaatg ataaaaacat caaaattgaa
     1861 atggatatca tgacttgggc caatttgaat gaaaaacttc ctacagcgat ttcttctaaa
     1921 aaagcacctg actttattgc attaaattat ccagattttg cacagtatgt agaaaacgga
     1981 gcggtacaac cattagacga tttctgggaa tatgatggag tagataaatc tgattttaaa
     2041 gaaacggcta ttgaattagg tgaagtagaa ggtaaacagt attttgtccc catgcaggta
     2101 caaggcatgt atatgtattg gaataatgat ttgtttaaac aagctggatt agatactgaa
     2161 caaccaccta ctacttggga agaattgaca gaaatggcac caaaattaac tgatagtagt
     2221 aaaaatgtat caggttttgt cttcaataag gatggaactg caccgttata taactggatg
     2281 ttagcaaatg gcggtaaatt agtgaacgat gattatacta agtctgaatt tgcgtctcct
     2341 gagaacctta aaacgttaaa agcgatccaa aaaatgatcc atgtagacaa gactggtcca
     2401 gaatcgattt ctggcgccga aatggacaat ttaatgaatg ctggtcaact agccattgaa
     2461 atcaatgggc catggttaaa taacggattg aaagcaaacg agattgatta taatgtgact
     2521 accttaccac aagggagtga ttcagacaaa aaaacagcta ttttagatgg cgtaggatat
     2581 gccattccgg caagtacaga taataataaa aaagaagctg tctatgagtt tttgaaatat
     2641 tggaatacaa ctgaaattgg taaaaaatgg agtatcgaaa atggttttcc tgcctacttg
     2701 gattcagtag caaaagatga agaagtaaaa aatgatccta ttgtatctga attatcgaaa
     2761 caaatggact atgcagagcc atatttacca ggttttaccc aaatctctgc gatcaataac
     2821 gatatcatca atccactgat tgaaaaatta ttagcaggag aagatcctga gacactcatg
     2881 aacgatgcag atgatgccat caatcaatta ctgcaaaaat agtacagaac tttatacagg
     2941 atagactgtt ggcaagatac agatatctga atgatcgacc tagtatttgc agataggtgt
     3001 tattgcaaaa atcacagcga ttgttcggat atcttattca gattcgttta aaccaagatt
     3061 gtatatttta tcaaaatgaa agatgaggtg gactatatga acggattcac caaatattgg
     3121 aatcgtcctg atcgtgctgc gtatttgttt ttaactccat cttttttagt tttattatta
     3181 tttactgtca taccgctttt aggaacattt gctattagtt ttacagattt gaatattttc
     3241 tttacatcaa gaaattttgt aagttttgaa aatttcacac gtatatttag tgatgaacga
     3301 gcagttaatt cgttgattca ttctttatat ttcaccgtac tcgaaacgcc aacacaaatt
     3361 atcgttggtt tattagcagc agtagcatta tctaaaaata ccagattcaa tcgtttttgt
     3421 cgttcgacat tttacattcc agttatttgt tcattgacgt caattgcgat tattttttct
     3481 atgttgcttg atccgaatgt agggacgatt ccttacttgt tttcacaagt cggcttgccg
     3541 atccctcaat tttttcgcga tccaaccctt gctatgccga cggtagccat catgactgtt
     3601 tggaagaatt ttggtgttac tatgacgatt ctcttgactg ctattcaggg aatctctccc
     3661 tctttgtatg aatctgctca aatggatggt gcaacaaatc gacagcagtt ttttaacatt
     3721 acgctacctc agatcgttcc ctccttaggt ttttgtattt taacgaatct gattggttcg
     3781 atgcaagtgt ttgaccaagt atatgttgca accgctggtg gcccacaatt taagacagaa
     3841 acagctgttc aatatattta cagtcgagga tttacagcac cttatgaatt gggctatgca
     3901 tcagcactgt cttcggtttt gtttatcatt gttgcaacta tagctatctc cactaatgtg
     3961 tacatgtcac ggaaagaaaa acaaatgaaa taggagggaa acctgtatgg caccagaaag
     4021 ctcgattaca gttactgtga atagtcgaaa agcagttagt ggtaaaaaaa tcattcgaac
     4081 gattagtatg ctagttatat tgttggttat tgcgtttcct tttttgtggc tgattatttc
     4141 ttcttttaaa cacgaaaaag acattatctc atttccgcct agaatatttg cagatagcta
     4201 taccctagat aactatatta aggtttggac aacgatccct ttattagatt acatcaaaaa
     4261 tacagtgatt tttgctggag ggacagtgat cacttctgta ttctttgatt ctcttgcggg
     4321 gtatgcattt gctcgtatgc gtttcaaagg aaaatcagtt ttgttctatt tcgttttgct
     4381 gacaatgatg atcccattcc aagtcttcat gattcctcta tttattgaag tcaatttgct
     4441 tggcatgctt gatacttatg caggtttgat cattcctcgt atgacaactg catttggtat
     4501 cttcatgatg cgttcctttt ttattacttt accagattct ttggaagagg cagcacgcat
     4561 tgatggatta agtgagttta atattttttt gaaaatcatg ttgccattga gcaaacctac
     4621 tcttttatca ttagggattt tcacgctgat gaatagttgg aatgacttgc tttatccatt
     4681 gatattgaca agtagttcaa aaatgagaac tttaccagca gggttagctt tatttacagg
     4741 gcagaacatt tctttttatg gacctgtaat ggctggaacg gttatttcaa tgctgccatt
     4801 actagtggtt tatatttttg cacagaaata ttttgttcaa ggaacagcta tgtctggaat
     4861 gaaagagtga ggaagatgaa aatagttgag actaaacttt atggacagtt gattgtggtt
     4921 tctgattaca tcttattagg tattctctgg gtaatcgtgt gtttgcctgt tattactatc
     4981 gtgcctgcta catgtgctgt tttttatgta atggcgcaat ggaaaggtga gggatcagga
     5041 aatatcattt attttttctt tcaaggaata aagcaacatt ttggtaagaa cttgttcctt
     5101 agtctgttaa cgatcattat ttattttctt gttaatcaat tattagaaga acagacaatc
     5161 gtactttcaa ttagtggata tgtagtttta cttatttatt ccatgtttct tttatcctgg
     5221 atatatgtga tttctgaaaa caaagatatc acatttttac aaatttttga acaaagtgca
     5281 tggaaagtat tttttgattt tttgagaaat attctttgtt gcgtattgtt attaggattt
     5341 gtactgctca tatttctttt ccctcctttt atttttatct ttgctggtgg cgtatggaaa
     5401 cttgtatcta ttatcttgac tataggaaat gaaaggtagg gaaagctgtt ggacgaagaa
     5461 gtgaaagtaa aaatcagaga ggattttacg gtaggagaga tcagttctcg gctgtttggc
     5521 tcatttgttg agcatatggg aagtgtcgtc tatacaggga tttatgaacc gggccatgaa
     5581 acagctgatg gtgacgggtt tagacaagat gtcctctctt atgtaaagga attagaatta
     5641 ggtgtcattc gctatcctgg aggaaacttt acatctggtt atgattgggt ggatactgta
     5701 ggtaggaaag aacaacgacc aaaaaaaatt gatttagcat ggcgaggtat agaaccaaat
     5761 tcttttggac tccacgaatt ttttaaatgg atcagtcatg tacaagcgaa accgatattc
     5821 acggtgaatt taggaacaaa aggaattgat gaagcaaaaa atttagtaga atattgtaat
     5881 tttccaaaag gaaccttctg gagtgattta agaataaaaa atgggcaaaa aaagcctttt
     5941 ggcgttaaat tatggtgttt agggaatgag ctagacggtc catggcaagt gggggctaag
     6001 agtgcttatg aatatggtag gttagcaaat gaagcctcaa aagtcatgaa gttagtggat
     6061 gattcaattg aaacaatctt agttggaagc tctacgccac gtttaccatc gtaccctgaa
     6121 tgggatcgta tcgcattaga agaagcttat gagaatgtag attatttagc tttgcataac
     6181 tatattgata agtatgatga agaagacttg actaaacctc ctaaaaatga gcgtgatgat
     6241 acagcgacat accttgcaaa agcttatcga ttcgatcgtc aaattgaaga aatcgttgct
     6301 acttgtgatt atgtaaaagg atatttgaga agtgagaaaa caatgtatct agcgtttgat
     6361 gaatggaatg tacatgccca accggaaaaa agacataggg attttgaagt cggtagtccc
     6421 atcgattggt gttactttac tatggaagat acattattat ttggttcatt agggctggct
     6481 attttacgac acgcagatcg cgtaaagatt agttgtcaat cattgttagt aaacacaata
     6541 cctttaatac tgacagatgc taaaggacaa gcatggagga atccaactta ttatgtgatg
     6601 caacacttat cgaaatatgc taaaggtcaa gtattgaaac aacaaatcat ttgccctgtt
     6661 tatgattgtg aaaagataaa aaatgttcct tatgtggaca gtgtgatcgt agataacggc
     6721 aatcagctga ctattttctt gatcaatcgc acaaatgaac aacaaaagat aacgattgaa
     6781 catgatttca atttgctaga tcaaggagag cactacacat taaccagtag cgatctgttg
     6841 gacaaaaata cacgtgaaga accagaaaaa ataaaacctg aaataaacta tttttcaata
     6901 gaaaaacatg acgaattaaa tgtagggata gaaaaatact cttggaatgt aattagaata
     6961 aagaaaatgt gaggaaattt gttatgatga agacacttta taataatcct atcgttcggc
     7021 aacgggcaga tccttgggta tataaacata cagatggtta ttattatttt acaggatcgg
     7081 ttccaggcta ccaagtgatt gaattaagaa gggcaaagtc actgaatgaa ttagaacatg
     7141 ctgcaagcct gattgtatgg caggctcatg aagaaggacc gatgagtgaa ttgatctggg
     7201 cacctgaagt ccattatatc aatggaaaat ggtatattta ctttgctgca agcaatcata
     7261 aaactatacg tgatgaatct catcatcacc gcatgttcgt acttgaaaat cagcatatag
     7321 atccaatgaa tacagattgg atagaaaagg gacaaataaa aacgaaaagg gaaactttca
     7381 gtttagacgg aacaactttc tcttctcaag gaaagctata ttatgtgtgg gcccaacaag
     7441 atcctgaaat tactgggaat tctaatcttt atctatccga aatggataat ccttggacgt
     7501 taaaggggaa gcagatcctc ctttctatac ctgagtatga atgggaaaaa cggggttttt
     7561 cagtaaatga aggtccagca gtccttactc gcaatgggaa aatattcatt acgtattctg
     7621 gtagtgcaac agatgaaaac tatgccatgg gcctgctgtg ggctgatgag cacaagaatc
     7681 ttttattaaa agaatcatgg cataaattat cggagccagt attcgtttct tctgaaaaaa
     7741 ataagcaatt tggtcctggt cacaactcgt ttacggtttc agaagacggg tgctttgata
     7801 ctttgatcta tcatgctcga ccagagaaga accaaaaagg ggatccgtta gacaatccta
     7861 atcggcacgc gaacgcgcaa tgctttacgt ggaatgagca aggatttcca gaatttggtg
     7921 aacctgttcc gtatgcctta tgagcacaag aaaccttgat ttttgcttga ataaaccttg
     7981 cagactcgtt atgatatatg tacgtacaaa taaaaagggg caaggcaaat ggcaaaatat
     8041 caagatattg cagaagagat aaaacaaaag atccttcgtg aggaatacga agtaaataca
     8101 accattccct cagaattcaa gctgcaagaa atatatgacg tcagcagaca tacgattcgg
     8161 caagcaatcg ctgtgttggt aaatgagggc tatctgcgta aagaaaaagg gtcgggcaca
     8221 tatgtagatg acagctataa gaaagaaaaa acaccattga acagaacaaa aacgatcggt
     8281 gtcatcacca catatctctc tgattatatt tttccgtcga ttatccgagg aatcgaaaat
     8341 gaattacgag aatctgggta ttcattgctt ttagccagta cgaataacga tcacgaattg
     8401 gagaaagagt gtttgaagaa aatgattgct tatggggtag atggtttgat cgttgagcct
     8461 acgaaaagta atcagtataa cccaaactta gcactttatg ttgctttgag agaacaaggc
     8521 attccgatgg tgatgatcaa tgctgcttat gaagagttaa atgcctctta tatttgttta
     8581 gatgatgtga aagtcggttt tatggcagca gaatatttga taaaaaaagg acatcagcgt
     8641 ctgttgttca tcacaaaaat cgacgatctt caaggaaaat acaggatgaa aggttttatc
     8701 agcgcgtgtg aacagtataa tgtgcgtttt ttgacggaag atatcattac tttcaccacg
     8761 gaaacacgag aaacaatcgc tgaaaaaaca gtacaacggt tgaaagaaga acaagcttta
     8821 gatggcatca tttgctataa cgatcaaata gctagtttaa tgctagataa gttgacagaa
     8881 aatggctatc atattccaga tgacctatcg atcgtaggaa cagacgattc ttctttaagc
     8941 catttaggga ataaaaaatt gacaacattg acgcatccaa aagaaaagtt gggacaagat
     9001 gccgcacggt ggatcgtcac aacgattgaa aaaggacagg cacagccaaa tattctctat
     9061 gaacctgtac tagttgaacg ggaaacagtg aaagaaaacg aaaaataaga gacgggtttt
     9121 taccgcctct tattgctcct acggttactc gtcgcaatgt ttgtgagagc ttttgtcatc
     9181 gctcaaggct gaacgtttct ttcaccacct ctccactaaa cggtgttctc ctaccaactg
     9241 gtcacaagtc acaatattca tcaaacatga gaagctgttt tttgaaattg ttccttgtga
     9301 ctggaaacta agcggttcct tcttatttct tgaagctgac cacttctgtc acaacctctt
     9361 taagaatgat ggatagaaat acgtttagga taagtttttt tagatgcact ttgttcgatt
     9421 tcctctaaag atttcccacg agtttctggt acgatctctt gaataaataa aacccctaaa
     9481 aagcagatga tcccaaaaat gccgaaaacg atttcttgag acatagaagc agtcatcaca
     9541 ggaaaaagca agccaactag gaaagaacca atccaattaa aagaagaggc taggccagat
     9601 gctcgcccgc ggacagctag tgggaatatt tcgcctacga ttacccatgt cagtggagcc
     9661 catgtgcaag aataaaatgc gacataaata cataaaaaca agagaatcaa caacgaattt
     9721 gtatgagcat ccagtacagt tcctaaaaca gctggtagga tgaaagaaag tcccatcacg
     9781 ctaccgccta ttttcaataa agttcgccga ttgaacttgt ctgcaataac caaaaagagc
     9841 aaagaaccag caactaaaat gattccttgg atgatcggcc acatcaatgc atcgctcgct
     9901 gcatgcccag tcgctttttc tacgatcagc ggaatatagt aaaaaatagc attagctcct
     9961 tgaaattgct ggaatactgc tacaccaata ccggcaatga ctaaggaacg atatttttct
    10021 aaaaacaaag tatgccaaga agttccggtt gtcttttctt gtttgacatt cagctggatt
    10081 tgttcgaatt cctgttcagc ttcttttggt gtccgaatat agttcaatac tctttttgcc
    10141 tcttccaagc ggccagattg cataagaaaa cgcggtgact ctggtagagc taaaacaccg
    10201 aaaaataaaa tcagcgcggg gacagcagcg agccctaaca tcacgcgcca agccatcgtt
    10261 tcaggcaacc cttttagcaa ataatctgca acataggaaa ggagcatgcc agaagcaatc
    10321 atcacttgat tgatccctga caatcttccc cttagtctag caggagccat ttcagacata
    10381 taagcaggaa ctagggcaga tgcagcgcca acagctaaac ctagaagaat ccttgctgca
    10441 atcagaaaat aagaaccatt gtgaggggca gatgcagaaa ggacagaacc agccataaaa
    10501 ataagtgcag aaagaagaat catttttcgt cgtccaagct tatcagaaag aagaccagat
    10561 aaagagccgc caaggatcgc acctaacatg accgaagacg tgatccatcc tatcaaagaa
    10621 gctttacctg ccaaacccca atcatgctgt aaaaaaggta aggcgccggt cataacacca
    10681 atatcatatc caaatagaat gccgccaaat gcgccaaaaa aataaataaa cgatgaggaa
    10741 actttttttt cttttttcat aagtattctc tccttctaga atttcgagaa ggcggttgat
    10801 cagattttgt aagcaatttc attttataac ttatgtacgt acaaaacaag tgttttttat
    10861 tgtattcttg taaataactg cttgacatgt acgtacaaaa gtatacaata atcattgtaa
    10921 gcgctactaa aaagacggtt tgatcaggtt tgacgagaag gagggagtta aatcattatg
    10981 aaggaagtaa atgcgcaaag tatgttagat gtcatagatg gtcgtacatc tttagggatt
    11041 gagtttggat caactaggat caaagctgta ttgattgatt catcttttac tcctattgcc
    11101 gctggtagtt atgagtggga aaacaaacta gaaaatggta tctggaccta ttcattagaa
    11161 gatatttgga aaggactgcg cgtcagttac cgaaaaatgg cagcagaagt attcgaaatc
    11221 tatggcgaga atcttacgac aatcgggtcg attgggttca gtgcaatgat gcacggttat
    11281 ctagcatttg atagcaagga acagttacta gtaccatttc gtacttggcg aaatacaatc
    11341 actgaagaag cagaacgaat attaacggaa aaatttcaat acaatattcc gcagagatgg
    11401 agtatcgctc atttgtatca atctattttg aataaagaag aacatgtgaa agaaattaat
    11461 tttttgacaa cgcttgcagg ttatgtccat tggcagctta ctggtgagaa agtactaggc
    11521 gtaggagatg catcaggcat gtttcctatc gatccttcaa cgaaaaccta ccatcaagaa
    11581 atgaaaaata tttttggtca gttaatcgaa aaagcgcacg tagcgattga cttggatcac
    11641 attttgccta aagttctagt agcaggagaa gaagctggca agttaagcga atcaggggct
    11701 tatctgatcg accctacggg aaatttgaga ccaggaatcc cggtttgtcc gccagaaggg
    11761 gatgcaggaa ccggtatggt cgctacgaac agtgtagcca aagggacagg gaacatttcg
    11821 gctggtactt ccgccttcgc tatgatcgta ttagaaaaag aactagaaaa tgtccatccg
    11881 gaaatcgaca tggtcactac accttcagga gaactagtag gcatggttca tacgaataac
    11941 tgctcttcgg atatcaatgc ttgggtcagt atttttgaag aatttaccaa ttctttgggt
    12001 atggagataa gcagagataa attattcacg gtattattca acaaagcgct agaaggcgat
    12061 acggattgcg ggggattact ttcttatggt tatttttccg gtgaaaacat tacaggagta
    12121 aatgaaggaa ggccattatt tgtacgtaca ccaggtagcc gttttgattt agcgaatttc
    12181 atgcggatcc atttagccag tgcttttggt gcaatgagaa tcggaatgga tatattgaaa
    12241 tctgagaatg tccaaattga tcgtttagtg gggcatgggg ggatttttaa aacaccagaa
    12301 gttggacagc ggattctagc atctgctatg gaggcaccag ttacagtgat ggacacagcc
    12361 ggagaaggtg gagcgtgggg aatcgcgtta ttagcagcct atatgatgga tcgacatggg
    12421 aagtctttag aaaacttctt ggcagaagac gtgtttggac aagaccaagg aatcaccatc
    12481 acaccttcag cagaagaaat agcaggctat cagatattta tgagacgtta ccgtgagggg
    12541 atcgacattg aaaaaacagc gattcatcaa ttgcaagaaa aaggagtgat agaaaatgct
    12601 ggaacaactg aaagaagaag tataccaagc aaatcttgac ttgcctaaac acggactcgt
    12661 caaatataca tggggaaatg tgagcgcttt tgatcctgaa acacgatatt tcgttatcaa
    12721 acctagcgga gtcagttatg aagagatgac agcagacgac atggtcgttg tggacttgga
    12781 caatcatatc attgaaggta aactcaatcc ctcttcggat acgcctactc atgctgtttt
    12841 atatcgttca tttcctcaaa tcggaggaat cgtccatact cactcgacat gggcgacgat
    12901 ctgggcacaa gcaggattgg atgttcctgc gatggggacg acgcatgcag atacttttta
    12961 tggatcggta ccttgtgctc gatttttgac acaacaggaa atcgacagcg ggtacgaata
    13021 tgaaacagga aaagttatca tagaaacatt caaagaacgc aaaattgatc ccttagcaat
    13081 tccaggggta cttttacacg gacacggtcc atttacttgg gggaaagatg cgcaaagtgc
    13141 ggtaatgaac gcagtcgtac tagatgaagt atgcaagatg aatttattta ccagacagct
    13201 caattcattt tcagaagagc tgcctcaacg gattttagat aaacattatt tacgaaaaca
    13261 cggaaaaaat gcgtattacg gacaaaacaa ataaaaggag tggctaatta tgttgaatat
    13321 tggagaaaaa gaattttggt ttgttgtagg atcacagcat ttatatgggg aagaagcact
    13381 gcgagaagta aagaaacatg cacaagaaat ggtcgatgaa ctgaatgaaa atggccaatt
    13441 gccttatcca atccgccttc aagaattagc agttactgct gatacgatca caaaaatcat
    13501 gaaagaggtc aattatcgag aagaagtagc aggtgtgatc acttggatgc atactttctc
    13561 accagcaaaa atgtggatcc gcgggacaaa gcttctacaa aaaccattgc ttcatttagc
    13621 tacgcaatat aacgaaagca ttccttggaa aacgatcgat atggatttca tgaatttaaa
    13681 tcaatccgct catggtgacc gcgaatatgg gttcatcaat gctcgattaa ataaacaaaa
    13741 taaaatcgtc gttggctact ggaaacgacc tgagatccaa aaagagattg ctgattggat
    13801 ggatgttgca gtagcctata atgaaagttt tggaataaaa gtggctcgtt ttggtgacaa
    13861 catgagaaac gtgggtgtca cagaaggaga caaagtggaa gcacagattc aatttggttg
    13921 gactgttgat tattttggta tcggagatct tgtacaagta atcgatcgcg tatcagacga
    13981 agaagtcgat caattatttg aagagtacaa ggaattgtat accttcgact atggtgatta
    14041 tgaagaaaag acatgggagg aacatgtaaa agtacaagct cagcaggaaa tcggtattcg
    14101 ccgtttctta gaagaaggag gctataacgc ttttacgact aattttgaag acctgtatgg
    14161 aatgaaacaa ctacctggac tagctgtaca acgtttgatg gcagaaggat acggttttgc
    14221 tggggaaggc gactggaaaa ctgcagcgat tgatcggttg ctgaaaatca tggctcgagg
    14281 gaaagataca ggattcatgg aagattatac ttacgagcta gctagtggcc aagaagcaat
    14341 cttggaatcc catatgatgg aagtagatcc gacgcttgct gcgacgaaac caagaatcgt
    14401 tgtttctcct ttatcaatgg gagatagaga agatccggcg cgtcttgtat ttgatgggaa
    14461 agcaggagaa ggggtagtcg tatcgatggc agatttcggt acgcattata aactgctgat
    14521 caatgaagta gaagcctttg aaccaacgac tgaagcacct aatttacctg ttgcacgtgt
    14581 gttatggaaa actaaaccga atttccatga aggtgtccat tcatggattc aggcaggtgg
    14641 cggtcatcat accgttgtct cgctaaatct aacaacagat caaattgaaa cgtgggcaaa
    14701 attagttgag ttggaaacag tggttattag ataaaaagag gttgtgaaaa agccacttag
    14761 ttttaagaaa taagaagaga ttgacgaaat ttgcttttca acttttttga aatttagtct
    14821 tattatcaat gtcagctaac ggaacaccat ttatcaagat atctcaccct caatattttg
    14881 ctctcgaaaa taaggcttat ggcagtaact tgtcatagcc ttatttttat cactcagcct
    14941 actctgctct aactgctctt tttgcttcta ggaagtcaaa tggtaaacag ttgtctaatt
    15001 gcagaaattt cgagatactt gttagtaagg agggggtttt atgtatcacg ttcatttgat
    15061 tgatgctatt ccagaacaag agatagaaaa gttacaaggg gaatatttca aaataataga
    15121 tgatttgcca gccgaaggaa tagttattcg tagtacgaaa gtgaaagatg aatggcttac
    15181 accagatttg ctagcaattt caagagctgg tgttggggtg aacacgatca acgttgaaaa
    15241 agcttctgaa aacgggacga tcgtcatgaa tacaccaggt gtaaatgcta atgcagtaaa
    15301 agagcttgtt ttgtgctgtt tgctcttatc ttcccgtccg ataatcgaag caagtcgtat
    15361 ggtacaaaca ctaacaggtc ctaatatttt agaacaggca gaaaacaaaa gaagtgctta
    15421 tgtgggacga gaattgcaag gcaaaaccat cggtcttctt ggtcttggag cgataggcac
    15481 aaaagtagcg ctaagttgct atagcttagg gatggatgtc ttgggttatt ctatccgcga
    15541 tgcccagttg gattatgttc ggcaagcaga tttagaaaca gttctttcta cctcggatta
    15601 catcgtagtc atgctgccgt tgacagaaga tacaaaagga ttgatcgatc aggcgaatat
    15661 agaaaaaatg aaaaaagatg ctgtacttat caatgtagga agaagtgaaa ttgttgataa
    15721 atatgctgtt atgcaagcat tggaaaaaca acatctggct aaatacttga ctgattttcc
    15781 agaagaagag ttcttagaaa atgaccgtat acttatgttg cctcatctag gggatcgact
    15841 caggaagctt tggcagacag cggacgtttg gcagttgaag ccttaaaaga ttatcttttg
    15901 ttcggtacgg ttagagaagc ggtcaattat ccatccgcac gaatgttttt ccaagcgccg
    15961 tttcgattca ctatatttta tcaaaagaaa accaatatat tagcagagat attttctctg
    16021 ctgaatgaga acgagctagc gatcgcagat atatcgcgta accataagaa tgagcatgtc
    16081 tacatattga ttgacataga cagtactgat tttgaacagc tcaatcaagt gaaacaacag
    16141 ctagaaaaaa tttctggagt acgaaaggtc cgcttattaa agaaaccaga aaggccataa
    16201 agaaagagaa tatttggatt tcattaattc ttctttccgt tctttacttt cgaatttcgt
    16261 acaactaaaa taagagaagc aacattcttt tattgataga aatgaaggag caagcatttg
    16321 aaaagatata taagcagact ttcgaccaaa cagaaaaaga ttttaaaagg aacagcagca
    16381 atcatcagta tcttttttct tgttgttttt agtaatttat ttttgcagtg gtgtcaaaat
    16441 gatctatccg tagaccttgc gttgaaattt gctttttctt ggcatacgga aaaattcttc
    16501 ttagcctgtc tcgtcttgct gattattctt atttttctga ttgcactagc aggatctgtc
    16561 cctttaggtt cccttacgta tgtcgtagcc atcggtgtat taggttttgc aaattatatg
    16621 aaaatgagtt atcggcaaga accgatttat ccagacgatt tgaagatgat cactgaaatc
    16681 ggattgctga aagatatgac cggcactatg ctgtttacag tgatcttagc tgccgcagga
    16741 accgtgatag gattgttttg ctggtacatg tttcgaagcc tgaaaaaagg aaggcgattc
    16801 caattgatcc gattaacgac actgcttgtg gcaattggac tgctagggta tatcagtaat
    16861 ttcaacaatc ccgataattt gttgcgaaaa gcatataata aaacggcact atggatacct
    16921 tacagccaga aaatgaacta ctataacact ggttttatcg gtgggttttt gtacaatctg
    16981 aaggtagaac caatggatga acctgaagga tattcgaaag aaaagatcaa agaaatcact
    17041 gaaaaatacc agaagctagc cgatgaaaaa aataaaactg tagaggaaga gtcgccgaac
    17101 atcgtgttcg tgatgagtga aagtttctca gatcctagtc gattaaacgg cgtagaagta
    17161 tctggtgagc cattggcaga ttattatgaa gtagctgatc aaacatatag tggcaatatg
    17221 ctttctcaaa actatggcgg tggaacagca aatatcgaat ttgaagcatt gactggtttt
    17281 tctatggcgt tatttaatgc gcaactcacc accccttaca cgatgcttgt accaaaaatg
    17341 gatcagcttc cttctatcgt ttcagcgtta aacgcacaat cataccagac gacagcgatc
    17401 catccataca atacttcgat gtataaaaga gaggatgttt atcagacatt aggatttgac
    17461 cagttcatca gcgagcgtac gatgacctat acggatacga tagagaataa tccatatatt
    17521 tctgatgagt cagcttacaa agaagtaatg acacttctca aggaagaaaa aacacctcag
    17581 ttcattcatt tagtcacgat gcagacacat atgccctacg atggaaagta tgataatcta
    17641 gactatcctg caaaaacttc agatggaagt gggacacttg acttagagaa ttatctgcaa
    17701 gatatttcct atagcagcac ggcactgaag cagttcacgg aagaattgaa gaatctttct
    17761 agaaggactt tagttgtttt ttggggggat catcttccag ggatctattc agatacgatc
    17821 caagcaaaaa atgacaaaca aacattgcat gaaacacaat ttcttatgtt cgacagtaaa
    17881 ggagaattag aaaagcaaac gactcacgat gcaatcacca gtccgtttta ttttgcagcg
    17941 aatctaatgg aacagacgaa tcagacaaca aatggctttt atcagctgct tttatcatta
    18001 gaacaggaac ttccggcgtt tgaacgagaa ctgtactatc aaaacggaca gtggtataaa
    18061 gaagcgcaat tcaatcgctc gcaacaagag atttacgacg aatatcaatt gatccaatat
    18121 gacattgtcg cagggaaaca atatagttta gcagatggtt tctttgagca cgaataagaa
    18181 tggcgagctg tatagaaacc attaaatttt ttcaagtact tgtcatttga ggataaaaac
    18241 aagtagaata aataataaca acaatggaga aagtaggaaa acgaacatgt taccgaatat
    18301 tggctattat gtgcagtcca tcgatcaaat cgtcaaggaa acagaagcaa tcggagaagg
    18361 aatgcatccg aaatatgaag tgatcagaaa cgcaattgat gaaggaaaaa caagtgaact
    18421 ggacaaatcg actcttgaag agatcatcct gttatttgaa gaaggaacaa aaaaatatcg
    18481 ggcaatgctt atgaaagtca gtactttgcg tccaccagca aaagcaatgg gcattcataa
    18541 aaattttgaa aaagcttatc tttcatatgt agcaggctgt gaagaaatga tccaaagtat
    18601 cgatcttgaa tcaggggtag atgaagatct attcaataca tctgaagcaa aacaagacca
    18661 agcgacagac caattgacag ctgccatcca aaaaatgact aatttgttgt tgaaaaaata
    18721 aaagaaatct ttgataacaa taaaaactag agttatctaa tataaatgta atattacctt
    18781 tatgatagag tgatcaaaat cctttatgat aatgacatac ctataaccaa caacaacctt
    18841 gtacttactc aaaatcgagt aaatactcct tttccgtcga gtctaaccat ctcggcggtt
    18901 ttttatttgt agaaaagaac atcaggatag acggaaaagt cttgtcctga tgttcttttc
    18961 agatagtatg gaaagctgat tagttttcta cattctgtac atattgttca aagtagttct
    19021 gtaaatcggc tttgagtttc tgatattcct cttcgctata ctctttatct tcgattttcc
    19081 catcaaagaa aggaatattt agttcttccc gtagtctgtc taatacgtct tgctgattca
    19141 tttcattctc cttttcttac taacgatcaa aataaacaca gacacattct ggtacataga
    19201 catctttttt cagtaattcc agtaatcctg tttcaggatg acggcgatat aatgtcagat
    19261 tatcggtgtt ctgattggca cacacgacaa aattattgct tggatctagt gcgaaatcgc
    19321 ggggaaaatc cccttctgtt gagatgattt gcttgttttg aatgtgggaa ccatcttcaa
    19381 aaactgcgaa tacagcaatc gagttatgtc ctcggttaga agcataaagg aacttgccat
    19441 catttgaaat gcggatagct gcccctccat tttcaccgtc gaattcttct ggcaaggtcg
    19501 atattttctg taattcttca aaagcacctg tttctttatt ataagaaagc acgcggactg
    19561 tgctgtctaa ttcaccaaat aaataagcga attgtccatt tggatgaaag acaagatggc
    19621 gagggcctgt tcctggctct gcagtgaagg tagttacaag attcaatttg ccgttttcgg
    19681 acacatcata agtatacaca cgatctgtac caagatcaca tacgactaat cgttggtcag
    19741 gagtcagatc cgtatagtgg acatgcgcat gatcttggtt tttatgagga cctgtcgctt
    19801 cttgatggaa tattgaatct gctgctttca gttcgccatc tcctaatata tggtagacat
    19861 ttacttcacc tttatgatag ttcgccccgt atagtaattg acgtgcttca tcaaccgcta
    19921 cataacacaa aggagcgcca gactctgtta ccgcattcaa aaaatgaaaa tccttatcgt
    19981 acgcaccggc accacctaat ccatccacac tagtaactgt gtaaagattg ccagctttac
    20041 tttttgctac ataggtaggg ctattttctt caacagctaa tgaaaggttg gctaattctt
    20101 ctttttctgt atcaaggtcg atcgtataga ttcctttact ttcgcgacgc gtataggttc
    20161 ctaaaattat cttttcgatc atatgagctg attcctccta attgtaatga aatagttttc
    20221 gtttttattc tatcacataa ttattccaca cggctaaaga atgagaatgt gttatactgt
    20281 ttttgaaagc aattaaagga gagagtgcaa tgattcaggc aaagattaca gaaattggga
    20341 cagaagcaat aaacaaggaa gaacccatgc tgattttgtt cgataaaaca gcaacagctg
    20401 ccttacgaaa atattcggtg attcaagaag tatcaaaaga cgaacctttt tctttgaaag
    20461 caggcggaac aattgctttt gatgatcagg tatatacgat cgattatgtc ggtccgatgg
    20521 ccaatgggaa tttaaagaca gtagctcacg tatcattgat ttttgatgct tgtccagaag
    20581 aaggacaaat tgcaaatgga atctatcttc atccgcatag actgcctgct attggtttgg
    20641 gaagccaaat tttttataac taacaggagg aattatggaa ggaaaaaata agaagaacag
    20701 accggtatcg tggttttggc gctggttttt aaataatcaa gtagtcacag ccttacttgt
    20761 tgtattgtta atattgctga tcgtcttttt atttacaaaa gtttcatatt tgtttgcacc
    20821 aatctggcaa tttttagcaa tcgtaggtct gcctatcatt ttagcaggaa tcttgtatta
    20881 cttgatgaat ccggtagttg attactttga aaagcgaaaa gtaccaaggc tttacagtat
    20941 cattgggctg tttgttttgg ttgttgcact aatcgcatgg gggagcgtag tgatcatccc
    21001 taagattcag gaacaaacag tgagcttcat cagtaacttc cctcaatatg tagatacgat
    21061 tgataataaa ttaaccgaga ttttgcggga cccgctgttc aaccaatttc gcgaacagtt
    21121 ggaaacagca ggtgataagt tcatgaattc ggcaggcgat atgatccaag atatatctaa
    21181 gagtacagtt cagagtcttg gaagttttgt cggcgcggta gcgactattt tagtggctct
    21241 gctgacgatg ccgtttatct tattttatct attgaaagac ggtaaacaat tagcacctta
    21301 ttttgtcaaa tttttaccga cacgcatgca aaaaccgacg ttgaatgtgc tgagtgaaat
    21361 gaatagtcaa gtttcttctt atataagagg acaactaact gtggcttttg ctgtggcaat
    21421 catgtttatg attggttttg cggtcattgg tttagagtat gctgtcacgt tggggattat
    21481 tgcgggtttt ttaaacctga tcccttactt aggatcattt ttagcgatga ttccggctat
    21541 ctttttagcc attgtagcag gtccattcat gatcatcaaa gtactgatcg tttttgcagt
    21601 agagcagaca atagaaggac gcttgatctc tccattagta ctagggaatc agttagcaat
    21661 ccatccagta acgattttat tagttctgct gacttctggg aaattgttcg gaatcatcgg
    21721 tgtgatcttg gggattcctg tttatgcagc agctaaagta gtgattactc atgtttttga
    21781 atggtacaca acaatctctt ctctttatga agaaaatccg ttgaaaaagc agaaagaata
    21841 acaaaaagag acagatggga aaatatccaa ggcacaaatg ctgctttgaa gattttttcc
    21901 tctgtctctt ttgtatgcat ttatctcaat ttttaaaatg atttacctaa agcagcagct
    21961 ttatctaatg cattttgcat caactcatct gcacggtcag ggtcataatc gattccttcg
    22021 atgaatagct gatctacttg atcgatgcct acaaagttta aaataccttt tacatattga
    22081 gaggcaaagt cttgtccgtt gtatacaccg ccattagatt ggatgtgcag tgctttttta
    22141 cctgtcacta atccttctgg cccgttttct gtatagcgga acgtttttcc agctacattg
    22201 acggtatcga accaagcttt taattttgtt gggatattta gattccataa ggcgtttgcg
    22261 ataacgattt tatcagcagc aaggaactga tcagtcaatt cattgaaacg agcaattttt
    22321 tgttgttgac tttctgataa ggaagtgaat tctgcaccag cacgtaatgc ttcccaacct
    22381 gataataatt cttcatcgat ttccggaata tcatcgcgat aaagttccaa aacagtcact
    22441 tcatcatctg gatgattttc tttataactt gttaaaaaag acgtcaatgc cttaactgtg
    22501 cgcgattctt ctgcagttag gggatgacct ttgactacta aaacagatgc catatgctta
    22561 ccttctcctt ttattgctat gattgttata actatgacta acaaaatcag tttactcgaa
    22621 aaagaaaagg ctatcaagtc atattgataa ggttgaagaa taaataacta aaaaaagaca
    22681 gggtataaaa gaggctgtga cacgattttt tgtcacagcc tcttttgaat ctaacaagca
    22741 atattcaagc aacactcgac tgaagagctt ccatcccacc aatgatataa acagcacgaa
    22801 aaccattagc agtaagttga tcggctaatg cttctgatcg cctgcctgta tagcttagaa
    22861 tataataaag tttctttgga tctagatttt ttaattgtct tggcaagata gcagcgggga
    22921 tagaaagcga gtggcttaag tggttttttg tgtaatctaa ggtatttctt gtatctaaaa
    22981 cgatgctgtc agcatcgaga ggcaatttat tgaagtcttg gacactaatt gattgggcca
    23041 tttatctcat cctttgttta gtatgtggct attttaatgg gcaactctca ttaattcaaa
    23101 tggaaggatt cctcttatca cttttttaat gtatcggatg ataatgaaaa aaactatgca
    23161 aatgtcttgc aaaacaggta ggaggtgtta taatgacata gcgtcatatg acaatgtgtc
    23221 atcttgaaag aaaggagaat ccatatgccg acacagacat ttttccattt gcctaaagaa
    23281 aaacaaaaaa gactaatcga ggctgcgaga atcgagtttt ctagagtacc cttaaaagaa
    23341 gcttcgattg ctaatatcgt caaattagcg gatatcccta gaggcagttt ctatcagtat
    23401 tttgaagata aagaggatct ttatttttat tactttgaga caatgagacg tgacagctct
    23461 aaagatatgt tacaactaat gaaagaatcc gatggtgatc tattcaaagg attcgaatcc
    23521 tatttttcta aaatgattta tgagattttg gaaggcgaga atgcttcttt ttatcgtaac
    23581 ttgtttatga acatggatta ccgtgcctca agaaaagtcg ctcctcatac gagtcgccaa
    23641 aaaacgcctc acgatccaca taaagaacac aaacaagatg caaaagagtt gcttgaattg
    23701 atcgatcgga cgaagctgaa agtaaaaaat gatcatgaat tggaattact gatccaatta
    23761 gtgatgaata ctgtatttac aacaattgcg cattcgtatc gcttattttc aacatctgaa
    23821 gattactcag tagagcaagc aatcagtgaa ttcaatttga aactttcttg gctgaaaaac
    23881 ggagtatata aatagaaaga agggaactag atgctaaaaa tcgtaaaacg gatctccttg
    23941 acttcagcaa ttgcagctgc agttttcatg gtgatccaag tactggcaga tttatatttg
    24001 ccgacactta cctccaatat tattgataaa ggggtagcgc aaggagatgt cgattatatt
    24061 tggcatacag gttttgtgat gatcggcttt tctttgatca gtatcttagc tgctatcgca
    24121 aatacatttt ttgctactcg tgaatcacag aaactgggta agcaattgcg tacggatgtc
    24181 tataaaaaat cagagagtct gacaaaagat gcgtttgata aatatggaac ggcctctttg
    24241 atcacaagaa cgaccaatga tgtgactcaa atccaaatgg ttacacaaat gtttttacgt
    24301 atgatgatca atgcaccaat cacgttgatt ggcgcgagca tacttgctta tcaaaaagat
    24361 catcagttga caaaaatctt cttagttgtc atcccagtaa tgattatttt gatcggcgga
    24421 atcatgtact ttgctgttcc attattcaaa agtatgcaaa agaaaactga ccgtttaaac
    24481 ctggtatttc gagaaggatt gacaggggtc agagtgattc gagcattcga taagacacgc
    24541 tttgaagaaa atcgttttga tttagcgaat aaagattata caaatacggc aatcaaagtt
    24601 aatacgatcg tcgcattgat gatgccaatg atgacattga tcatgagcgg tacaaacgta
    24661 gcaatcactt ggtttggtgg acactacata gcagatatga ctttggaagt cggtaatctg
    24721 attgcattta tgacttatgc gatgcagatc ttgatcagct ttatgatgct ttccgctatt
    24781 tttatcatgg tgcctcgtgc acaagcttca gctgaccgta ttaatgaagt gttggatgaa
    24841 aaaatcggta tccatgaccc tgaaaatcct aaaacagttt cttttgctgg aaaaaacgca
    24901 acattggcct tcaatcatgt caattatcga tatcatggag cagaaaaact tgcattggaa
    24961 gatattgatt tccaagccaa gtcgggcgag atcgtcgcaa tcataggggg gactggttca
    25021 ggtaaaacga ccttagtcaa tttgatccct cgattatatg acatcgaatc tggttcgatc
    25081 caaataaatg gaacggacat tagtgacatg actcaatata atttgcgaga attaatgggc
    25141 tttgttcctc aaaaagcagt tctgttctct ggaacgatcc gagacaatat gcagtatggt
    25201 aagccggatg caacagatga aatgatttgg aaagcattag aaatcgcaca agctaaagat
    25261 tttgtttcag agatggaaga tggcttagat agccatgtgg aacaaggagg aggaaacttc
    25321 tctggtggac aaagacagcg tctggcaatt gcacgggcat tagtcaaaac agcagatatc
    25381 tatgtgtttg atgattcctt ctcagcgttg gatttcaaaa cggatgccaa tcttcgccaa
    25441 gcattgaaaa cgaatatgaa agaaagtatc accgttttag ttgctcaacg agtaagtaca
    25501 gtaatggatg cagatatgat ccttgtgcta gatgaaggaa aactggttgg caaaggaaca
    25561 catgaagaac tattagcaac aaacgaaact tatcaagaaa tcgttcattc acaactaaga
    25621 gaggaggact tagcatgagt aaaacaaaca cacgtccctc tcatggacca gcaggcggtc
    25681 caggacatgg tggacctggc agaaatctcg gtgtaaaagg tgaaaagcct aaaaatttct
    25741 ggggaacggt acgccgattg tttggctata tgtctaaacg tatggttgcg atcatcgctg
    25801 tatttgtact agcaatagca gccgtgatct tccaaatcca aacaccaaag gtcttgggga
    25861 aagcaaccac agaaatattc aaaggtgtaa tggctggcgc tcaacagatg caacaaggcc
    25921 aacaagtaac aaaatttccg atagactttg ataaaatcgc gcagatcatt gcgacagtaa
    25981 tcgtgatgta tttgatttct gctgtattca actttttaca gcaatttatt atgacacgtg
    26041 tttcccaacg aacagtttat gaattacgtc atgatttgga agccaaaatg aatcgtgtac
    26101 cgatttccta ttatgataca catacaaatg gggatatcat gtctcgagca atcaatgata
    26161 tggataacat cgcaagcacg ctgcagcaaa atttgactca atttgtaact agtgcagtca
    26221 cattgattgg ggtcatcgtg atgatgcttt caatcagctg gcaattgacg ttagtcgcgt
    26281 tattgatgat ccctttaagc ttgatcatcg tgatgatcgt agcgccgaaa tcacaagtgt
    26341 ttttcgcaga tcagcaaaaa agtttaggtt tattgaataa ccaagtagaa gaaacatatg
    26401 gtggtcatac cattgttaaa acattcaatc atactgaaaa agaccaggaa gtatttgaaa
    26461 aagaaaatca gaatctgtat gccgctggtt ggaaagctca atttatttcg gcaatcatca
    26521 tgccgttgat gaatttcgtt aaaaatctag gctatgtgtt cgtcgctgta ctaggcggag
    26581 taaaagttgc aaatgggaac atgacattag gggatgttca agcgttcttg caatatacaa
    26641 cacagttctc tcaaccaatc actcaacttg caagtttgat gaacactatc caatcaacag
    26701 ttgcttcagc cgaacgggta tttgaagtgt tggatgaaga agagatgtcc aatgaaaaat
    26761 ctggccttcc agaagaaaaa gatacaccat acaaagttcg ttttgaaaat gttcaattcg
    26821 gctacacacc agataacttg ctaatgactg attttaactt ggatgtcaaa cctggtgaga
    26881 tggtcgctat tgtaggtccg actggtgcag ggaagacgac actgatcaac cttcttgaac
    26941 ggttctacga tgtttctgga ggtagtattc gttacgatgg tgtagatacc cgtgatcttt
    27001 cacgtgatga attgcgcgca cagttttcaa tggtactgca agatacttgg ctgtttacag
    27061 gaagtatcta tgacaatatc aaatatggga acgacgatgc aacagacgaa caaatcattg
    27121 aggctgctaa agctgctcac gtagacgatt ttgttcgtaa attgccagaa ggatataaca
    27181 ctgtattaaa cgaggatgca agcaatattt cgcaagggca gcgtcagcta atcacaattg
    27241 cgcgggcatt cttagcaaat ccagatgtcc tgatccttga tgaagcaaca tctagtgtag
    27301 atacacgaac agaaatactg attcaaaaag cgatgaaccg cctattggaa aaccggacaa
    27361 gtttcgttgt tgctcaccgc ttgtctacaa tacgcgatgc agacaacatt attgtgatga
    27421 atcatggttc gatcgtagaa acaggaaacc acgatacatt gatggaaaaa gacggattct
    27481 atgcagatct ttataacagt cagttttctg aagaagaagc ttcatagaat agtacaaatt
    27541 tattattttt tttaggggct gtgacaaaag tcgtgtcaca gtcccttttt ccgaataaac
    27601 ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac ggcaaaatat gagaagctat
    27661 ttttccgttt tgtatctcta atactcaggt caaaccgctg attttacaac ctcttcctta
    27721 aacggtgttc tcctgccaac tcctcggtca caagtcacaa tagtcaacaa acctgagaag
    27781 ctgttttttg aaattgctcc ttgtgactcg gagttaaacg gcaggctcac aacctcttgg
    27841 taaacggtgt tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa
    27901 agccattttc acaaaatttt tcttactact cgaaactaag cgactttttc acaacctctt
    27961 ccttaaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag
    28021 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
    28081 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
    28141 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
    28201 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
    28261 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
    28321 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
    28381 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
    28441 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
    28501 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
    28561 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
    28621 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
    28681 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
    28741 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
    28801 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
    28861 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
    28921 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
    28981 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
    29041 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
    29101 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
    29161 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
    29221 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
    29281 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
    29341 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
    29401 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
    29461 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
    29521 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
    29581 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
    29641 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
    29701 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
    29761 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
    29821 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
    29881 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
    29941 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
    30001 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
    30061 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
    30121 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
    30181 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
    30241 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
    30301 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
    30361 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
    30421 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
    30481 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
    30541 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
    30601 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
    30661 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
    30721 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
    30781 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
    30841 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
    30901 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
    30961 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
    31021 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
    31081 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
    31141 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
    31201 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
    31261 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
    31321 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
    31381 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
    31441 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
    31501 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
    31561 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
    31621 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
    31681 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
    31741 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
    31801 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
    31861 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
    31921 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
    31981 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
    32041 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
    32101 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
    32161 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
    32221 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
    32281 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
    32341 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
    32401 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
    32461 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
    32521 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
    32581 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
    32641 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
    32701 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
    32761 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
    32821 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
    32881 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
    32941 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
    33001 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
    33061 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
    33121 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
    33181 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
    33241 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
    33301 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
    33361 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
    33421 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
    33481 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
    33541 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
    33601 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
    33661 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
    33721 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
    33781 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
    33841 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
    33901 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
    33961 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
    34021 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
    34081 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
    34141 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
    34201 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
    34261 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
    34321 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
    34381 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
    34441 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
    34501 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
    34561 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
    34621 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
    34681 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
    34741 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
    34801 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
    34861 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
    34921 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
    34981 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
    35041 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
    35101 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
    35161 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
    35221 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
    35281 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
    35341 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
    35401 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
    35461 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
    35521 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
    35581 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
    35641 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
    35701 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
    35761 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
    35821 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
    35881 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
    35941 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
    36001 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
    36061 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
    36121 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
    36181 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
    36241 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
    36301 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
    36361 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
    36421 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
    36481 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
    36541 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
    36601 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
    36661 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
    36721 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
    36781 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
    36841 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
    36901 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
    36961 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
    37021 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
    37081 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
    37141 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
    37201 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
    37261 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
    37321 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
    37381 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
    37441 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
    37501 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
    37561 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
    37621 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
    37681 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
    37741 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
    37801 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
    37861 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
    37921 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
    37981 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    38041 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    38101 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    38161 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    38221 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    38281 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    38341 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    38401 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    38461 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    38521 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    38581 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    38641 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    38701 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    38761 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    38821 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    38881 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    38941 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    39001 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    39061 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    39121 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    39181 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    39241 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    39301 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    39361 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    39421 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    39481 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    39541 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    39601 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    39661 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    39721 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    39781 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    39841 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    39901 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    39961 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    40021 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    40081 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    40141 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    40201 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    40261 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    40321 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    40381 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    40441 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    40501 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    40561 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    40621 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    40681 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    40741 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    40801 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    40861 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    40921 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    40981 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    41041 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    41101 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    41161 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    41221 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    41281 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    41341 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    41401 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    41461 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    41521 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    41581 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    41641 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    41701 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    41761 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    41821 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    41881 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    41941 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    42001 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    42061 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    42121 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    42181 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    42241 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    42301 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    42361 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    42421 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    42481 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    42541 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    42601 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    42661 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    42721 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    42781 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    42841 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    42901 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    42961 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    43021 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    43081 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    43141 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    43201 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    43261 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    43321 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    43381 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    43441 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    43501 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    43561 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    43621 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    43681 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    43741 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    43801 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    43861 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    43921 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    43981 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    44041 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    44101 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    44161 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    44221 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    44281 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    44341 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    44401 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    44461 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    44521 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    44581 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    44641 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    44701 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    44761 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    44821 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    44881 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    44941 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    45001 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    45061 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    45121 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    45181 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    45241 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    45301 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    45361 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    45421 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    45481 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    45541 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    45601 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    45661 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    45721 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    45781 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    45841 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    45901 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    45961 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    46021 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    46081 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    46141 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    46201 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    46261 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    46321 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    46381 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    46441 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    46501 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    46561 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    46621 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    46681 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    46741 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    46801 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    46861 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    46921 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    46981 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    47041 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    47101 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    47161 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    47221 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    47281 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    47341 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    47401 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    47461 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    47521 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    47581 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    47641 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    47701 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    47761 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    47821 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    47881 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    47941 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    48001 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    48061 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    48121 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    48181 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    48241 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    48301 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    48361 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    48421 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    48481 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    48541 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    48601 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    48661 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    48721 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    48781 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    48841 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    48901 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    48961 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    49021 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    49081 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    49141 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    49201 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    49261 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    49321 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    49381 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    49441 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    49501 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    49561 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    49621 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    49681 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    49741 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    49801 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    49861 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    49921 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    49981 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    50041 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    50101 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    50161 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    50221 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    50281 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    50341 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    50401 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    50461 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    50521 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    50581 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    50641 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    50701 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    50761 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    50821 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    50881 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    50941 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    51001 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    51061 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    51121 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    51181 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    51241 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    51301 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    51361 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    51421 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    51481 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    51541 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    51601 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    51661 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    51721 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    51781 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    51841 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    51901 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    51961 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    52021 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    52081 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    52141 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    52201 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    52261 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    52321 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    52381 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    52441 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    52501 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    52561 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    52621 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    52681 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    52741 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    52801 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    52861 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    52921 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    52981 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    53041 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    53101 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    53161 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    53221 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    53281 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    53341 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    53401 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    53461 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    53521 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    53581 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    53641 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    53701 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    53761 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    53821 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    53881 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    53941 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    54001 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    54061 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    54121 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    54181 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    54241 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    54301 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    54361 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    54421 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    54481 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    54541 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    54601 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    54661 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    54721 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    54781 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    54841 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    54901 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    54961 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    55021 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    55081 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    55141 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    55201 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    55261 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    55321 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    55381 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    55441 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    55501 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    55561 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    55621 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    55681 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    55741 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    55801 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    55861 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    55921 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    55981 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    56041 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    56101 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    56161 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    56221 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    56281 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    56341 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    56401 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    56461 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    56521 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    56581 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    56641 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    56701 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    56761 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    56821 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    56881 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    56941 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    57001 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    57061 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    57121 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    57181 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    57241 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    57301 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    57361 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    57421 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    57481 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    57541 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    57601 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    57661 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    57721 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    57781 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    57841 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    57901 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    57961 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    58021 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    58081 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    58141 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    58201 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    58261 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    58321 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    58381 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    58441 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    58501 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    58561 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    58621 gtcgctatct cttgaaacgt tcttagaat
//
LOCUS       DAYYGS010000006        54857 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000006 DAYYGS010000000
VERSION     DAYYGS010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 54857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.006"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            57..130
                     /locus_tag="ACHA44_000347"
     tRNA            57..130
                     /locus_tag="ACHA44_000347"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:91..93,aa:Arg,seq:tct)
     gene            289..648
                     /locus_tag="ACHA44_000348"
     CDS             289..648
                     /locus_tag="ACHA44_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFM8903672.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            771..2402
                     /locus_tag="ACHA44_000349"
     CDS             771..2402
                     /locus_tag="ACHA44_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HFM8903673.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQFPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(2464..3105)
                     /locus_tag="ACHA44_000350"
     CDS             complement(2464..3105)
                     /locus_tag="ACHA44_000350"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8903674.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    3291..3367
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            3387..3695
                     /gene="rplU"
                     /locus_tag="ACHA44_000351"
     CDS             3387..3695
                     /gene="rplU"
                     /locus_tag="ACHA44_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFM8903675.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            3722..4066
                     /locus_tag="ACHA44_000352"
     CDS             3722..4066
                     /locus_tag="ACHA44_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HFM8903676.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            4079..4372
                     /gene="rpmA"
                     /locus_tag="ACHA44_000353"
     CDS             4079..4372
                     /gene="rpmA"
                     /locus_tag="ACHA44_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFM8903677.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4806..7612
                     /locus_tag="ACHA44_000354"
                     /pseudo
     CDS             4806..7612
                     /locus_tag="ACHA44_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            7803..8294
                     /locus_tag="ACHA44_000355"
     CDS             7803..8294
                     /locus_tag="ACHA44_000355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8903678.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            8397..9386
                     /locus_tag="ACHA44_000356"
     CDS             8397..9386
                     /locus_tag="ACHA44_000356"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIB"
                     /protein_id="HFM8903679.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            9419..10222
                     /locus_tag="ACHA44_000357"
     CDS             9419..10222
                     /locus_tag="ACHA44_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFM8903680.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            10243..11157
                     /locus_tag="ACHA44_000358"
     CDS             10243..11157
                     /locus_tag="ACHA44_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8903681.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            11240..11614
                     /locus_tag="ACHA44_000359"
     CDS             11240..11614
                     /locus_tag="ACHA44_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HFM8903682.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGNNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11753..11998
                     /locus_tag="ACHA44_000360"
     CDS             11753..11998
                     /locus_tag="ACHA44_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8903683.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            12170..13234
                     /locus_tag="ACHA44_000361"
     CDS             12170..13234
                     /locus_tag="ACHA44_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFM8903684.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            13480..13905
                     /locus_tag="ACHA44_000362"
     CDS             13480..13905
                     /locus_tag="ACHA44_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8903685.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            13898..14347
                     /gene="nusB"
                     /locus_tag="ACHA44_000363"
     CDS             13898..14347
                     /gene="nusB"
                     /locus_tag="ACHA44_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFM8903686.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            14444..15298
                     /locus_tag="ACHA44_000364"
     CDS             14444..15298
                     /locus_tag="ACHA44_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HFM8903687.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            15299..16636
                     /gene="xseA"
                     /locus_tag="ACHA44_000365"
     CDS             15299..16636
                     /gene="xseA"
                     /locus_tag="ACHA44_000365"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFM8903688.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            16641..16865
                     /locus_tag="ACHA44_000366"
     CDS             16641..16865
                     /locus_tag="ACHA44_000366"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFM8903689.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            16867..17757
                     /locus_tag="ACHA44_000367"
     CDS             16867..17757
                     /locus_tag="ACHA44_000367"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8903690.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            17766..18584
                     /locus_tag="ACHA44_000368"
     CDS             17766..18584
                     /locus_tag="ACHA44_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HFM8903691.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            18691..19140
                     /locus_tag="ACHA44_000369"
     CDS             18691..19140
                     /locus_tag="ACHA44_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8903692.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            19158..20840
                     /gene="recN"
                     /locus_tag="ACHA44_000370"
     CDS             19158..20840
                     /gene="recN"
                     /locus_tag="ACHA44_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFM8903693.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(20895..21011)
                     /locus_tag="ACHA44_000371"
     CDS             complement(20895..21011)
                     /locus_tag="ACHA44_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HFM8903694.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(21084..22040)
                     /locus_tag="ACHA44_000372"
     CDS             complement(21084..22040)
                     /locus_tag="ACHA44_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8903695.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            22211..22579
                     /locus_tag="ACHA44_000373"
     CDS             22211..22579
                     /locus_tag="ACHA44_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HFM8903696.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            22749..23180
                     /gene="mraZ"
                     /locus_tag="ACHA44_000374"
     CDS             22749..23180
                     /gene="mraZ"
                     /locus_tag="ACHA44_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFM8903697.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            23201..24160
                     /gene="rsmH"
                     /locus_tag="ACHA44_000375"
     CDS             23201..24160
                     /gene="rsmH"
                     /locus_tag="ACHA44_000375"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFM8903698.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            24165..24566
                     /gene="ftsL"
                     /locus_tag="ACHA44_000376"
     CDS             24165..24566
                     /gene="ftsL"
                     /locus_tag="ACHA44_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFM8903699.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            24567..26759
                     /locus_tag="ACHA44_000377"
     CDS             24567..26759
                     /locus_tag="ACHA44_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8903700.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            26785..27747
                     /gene="mraY"
                     /locus_tag="ACHA44_000378"
     CDS             26785..27747
                     /gene="mraY"
                     /locus_tag="ACHA44_000378"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFM8903701.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            27761..29122
                     /gene="murD"
                     /locus_tag="ACHA44_000379"
     CDS             27761..29122
                     /gene="murD"
                     /locus_tag="ACHA44_000379"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFM8903702.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            29148..30236
                     /gene="murG"
                     /locus_tag="ACHA44_000380"
     CDS             29148..30236
                     /gene="murG"
                     /locus_tag="ACHA44_000380"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFM8903703.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            30251..31471
                     /locus_tag="ACHA44_000381"
     CDS             30251..31471
                     /locus_tag="ACHA44_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFM8903704.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            31627..32952
                     /gene="ftsA"
                     /locus_tag="ACHA44_000382"
     CDS             31627..32952
                     /gene="ftsA"
                     /locus_tag="ACHA44_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFM8903705.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            32975..34216
                     /gene="ftsZ"
                     /locus_tag="ACHA44_000383"
     CDS             32975..34216
                     /gene="ftsZ"
                     /locus_tag="ACHA44_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFM8903706.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            34233..34910
                     /locus_tag="ACHA44_000384"
     CDS             34233..34910
                     /locus_tag="ACHA44_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFM8903707.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            34924..35523
                     /locus_tag="ACHA44_000385"
     CDS             34924..35523
                     /locus_tag="ACHA44_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HFM8903708.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            35604..35825
                     /locus_tag="ACHA44_000386"
     CDS             35604..35825
                     /locus_tag="ACHA44_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HFM8903709.1"
                     /translation="MVYALLSWFPGGYQSGLGRLLAKICEPYISLFDRLPLRIGPLDF
                     TILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            35874..36656
                     /locus_tag="ACHA44_000387"
     CDS             35874..36656
                     /locus_tag="ACHA44_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFM8903710.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            36677..37384
                     /locus_tag="ACHA44_000388"
     CDS             36677..37384
                     /locus_tag="ACHA44_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HFM8903711.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            37646..40444
                     /gene="ileS"
                     /locus_tag="ACHA44_000389"
     CDS             37646..40444
                     /gene="ileS"
                     /locus_tag="ACHA44_000389"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFM8903712.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(40493..42019)
                     /gene="zwf"
                     /locus_tag="ACHA44_000390"
     CDS             complement(40493..42019)
                     /gene="zwf"
                     /locus_tag="ACHA44_000390"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFM8903713.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(42034..42681)
                     /locus_tag="ACHA44_000391"
     CDS             complement(42034..42681)
                     /locus_tag="ACHA44_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8903714.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(42865..43194)
                     /locus_tag="ACHA44_000392"
     CDS             complement(42865..43194)
                     /locus_tag="ACHA44_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HFM8903715.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            43371..44099
                     /gene="sfsA"
                     /locus_tag="ACHA44_000393"
     CDS             43371..44099
                     /gene="sfsA"
                     /locus_tag="ACHA44_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFM8903716.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            44115..45128
                     /locus_tag="ACHA44_000394"
     CDS             44115..45128
                     /locus_tag="ACHA44_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8903717.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            45128..46405
                     /locus_tag="ACHA44_000395"
     CDS             45128..46405
                     /locus_tag="ACHA44_000395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8903718.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            46468..49170
                     /locus_tag="ACHA44_000396"
     CDS             46468..49170
                     /locus_tag="ACHA44_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8903719.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            49322..49639
                     /locus_tag="ACHA44_000397"
     CDS             49322..49639
                     /locus_tag="ACHA44_000397"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8903720.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            49669..49989
                     /locus_tag="ACHA44_000398"
     CDS             49669..49989
                     /locus_tag="ACHA44_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8903721.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            50097..51557
                     /locus_tag="ACHA44_000399"
     CDS             50097..51557
                     /locus_tag="ACHA44_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8903722.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            51625..51846
                     /locus_tag="ACHA44_000400"
     CDS             51625..51846
                     /locus_tag="ACHA44_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903723.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            51877..52059
                     /locus_tag="ACHA44_000401"
     CDS             51877..52059
                     /locus_tag="ACHA44_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HFM8903724.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            52059..52472
                     /locus_tag="ACHA44_000402"
     CDS             52059..52472
                     /locus_tag="ACHA44_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HFM8903725.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            52595..53776
                     /locus_tag="ACHA44_000403"
     CDS             52595..53776
                     /locus_tag="ACHA44_000403"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903726.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(53847..54011)
                     /locus_tag="ACHA44_000404"
     CDS             complement(53847..54011)
                     /locus_tag="ACHA44_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903727.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            54165..54238
                     /locus_tag="ACHA44_000405"
     tRNA            54165..54238
                     /locus_tag="ACHA44_000405"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:54199..54201,aa:Arg,seq:cct)
     gene            complement(54307..54498)
                     /locus_tag="ACHA44_000406"
                     /pseudo
     CDS             complement(54307..54498)
                     /locus_tag="ACHA44_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT        18116 a         9282 c        11826 g        15633 t
ORIGIN      
        1 tcgtattgac agaagcaatt taattatcta taattattga tgttccgctg attcttgtca
       61 tagtagctca gctggataga gcatccgcct tctaagcgga cggtcggggg ttcgaatccc
      121 tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta acggtttttc tttgtttttt
      181 gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa aaacatcctt atcgagtaca
      241 attataagga gcaaaaaaat aaaactcatc agaaaagagg aagaagatat gacgatacta
      301 tctagaattt tagtgactct agttgcaatt gaattttttt atatcatgta tatcgaaacg
      361 gtgagaactg attctgacac gacaagtagg gtttttaaaa tgagtaaaga agagttaagc
      421 agaaaatctg ttcaaacatt gtttaagaac caaggagtgt acaatggact attaggagtg
      481 ggactacttt atggagctta tctttcttcc gcatctaaag aaatcacaag tatgctatta
      541 atatctattt tttttgttgc attatatggc agtttgacta gcgatcgttg gattttactc
      601 aaacaaggtg ggctagctct gttggcattg ctttctattt tcttataaat atagaaaaaa
      661 taagaattta tctaacagga aactttgtga aaaatattac tttatctcat gaaatatgat
      721 attctaatga atggtatttt agaaaatttc atgaaaaagg agttagatag gtgttcaaaa
      781 attatgtttc ctcactaaaa aaagaatttt caggttatga tctttcaaga tttcagaaag
      841 atcttttggc tgggatcaca gtagctgctg tagcattgcc tttagccttg gcttttgggg
      901 tatcaagcgg agctgatgcg gccgcaggtt tgattacggc tatcattgcc ggtatcgtta
      961 ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta
     1021 tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt attgcttgct acttttttag
     1081 cgggtctatt tttacttgca gcaggacttt tccgattagg aacactgact tcttttatcc
     1141 cagcacccgt aattacgggg tttacttcag gtattgctat cattattgca ttaggtcaga
     1201 tcgataattt atttggtgtt cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt
     1261 atcaaacgat aggatttccg ataaacacag cctctttgat catgggaggg ctagtcattt
     1321 tagggatgtt tatctatcca aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta
     1381 tcattttggc aacagcagtt atgctattgt tccatttacc agtagcaact gtgggtaaga
     1441 ttccacaaac attgatcagt agtaatcgtt tgaatatggg agattttagc ttttctgcct
     1501 tgcaacaagt ggcagttcca gctattagca ttgctttatt aggaatgatt gaaagcttgc
     1561 tatgtggtgc ttcggctggc cgaatggcaa ataagccctt agatagcaat caagaattaa
     1621 tggcgcaagg aattggaaat ctgttgcttc ctttctttgg cgggattcct gctactgcgg
     1681 ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca aactagatta gctggtatga
     1741 tacatgcagt tgtcttactg ctctccatgc ttgtttttgc gccagtgatg tcacagtttc
     1801 caatgccagc acttgcaggt gttttgattg tgacggcttg gcgtatgaat gaatgggaaa
     1861 cgatcaagga gctatttact aataaatatt ggtcagcagt tctattgttt atccttacga
     1921 tgatttgtac agttatcttt gatttaagta ttgctatcgt gatcggggtg atcggcggct
     1981 gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc attagaaacg atcgattggc
     2041 aaagaatggg gctgccagaa acggaaaagc tgaataactg gacagtagtg tatattagtg
     2101 gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc
     2161 acgatggtgt cattttctcc atgcgaggcg taccaagtat tgatttgacg gcacgtgcag
     2221 tttttgatga atttcaagaa aaagcagagc aaagaggaca aacgattatc tatacttcct
     2281 tgcagccaga agtagaaaaa caactttctc acttgtggga acatacattc acgcatcaac
     2341 atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa atatactgca attagccatt
     2401 aaaacaatga aaaagaacag acaagactgt aaactgaaga ttttatggtc ttgtctgttc
     2461 tttttaatat tgctgtaaaa gtgaagctag tacttgccag tcttgatgga taacaggtac
     2521 aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt ggaagaattc gatagttttt
     2581 ttctgaccat tcgtaggcat ccttttgttc tgttaattgt aagacaggcc ctgtatagag
     2641 ttgaccatga tttttggtaa ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt
     2701 tccaagtgtc gcaattaatt ttggctggac cttcatcagt tcgtaatcca atattggtgc
     2761 atgcgcaaat acttcggaac gagtaggtgt ccgatttgga taaacagttt ctgactgttg
     2821 tgtccgttta ttgatccgat gagtcacgcg atacgggcgg ctccttacag cactggtgat
     2881 atacacctct tctcgtgtta agccagttga agaaagtgcc tgcatcaatt cttttccagc
     2941 ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc cctggggctt ctcccacgag
     3001 catcagttta ggatgaagag gtccttgccc agcgataaat ccttctacag gacggccgtt
     3061 cattcttaaa gaagcttctt gtatcagttc ttttgggtat tccatttatg ctcctccttt
     3121 ttcttttatt ataagaataa aagaaacaca atgcgactat tttatttggt agaactgtga
     3181 ggatagaaaa gcagaagtat tgcttgaatt tttgttttac tttttgattt taaatttagt
     3241 tgactaacgg ttgctgttct ggtaagctag tactgttgta attgtggact ccacagctac
     3301 aaccgctcag aacaagtaat aagcacagca tatgctgtca cttcggatgg cgagtctaag
     3361 tatatcagaa ggaggtgctt tatagcatgt acgcaattat taaaactggt ggtaaacaag
     3421 taaaagttga agtgggacaa gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa
     3481 aagttgtttt tgacgaagtt atcttagtag gcggagaatc tacaaaagta ggcgctccaa
     3541 ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg
     3601 ttactttcaa atacaaacct aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat
     3661 atacaaaagt ggttatcgac gcgatcaacg cttaatagcg tttgagaaag aaggcctata
     3721 tatgatcaaa ggaacattta agcgaaatga cgcaggtcag atcgtttcat tcactttgac
     3781 cgggcatgct gatgctggtc cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt
     3841 agcgatcagc actgtcaacg ggattgcctc attagccggt tttgagccga ttgttgaaat
     3901 gaatgaagaa gaaggcggtt atctttatac cgaagtcact tcaggaatga cacaagaaca
     3961 aaacaatatt gctgagattc tattagaaaa tcttttatta ggattacaat cgatagaagc
     4021 tgaaaactca gaatatatcc aaattaaaac aattaatgat aaataggagg tgcagaccat
     4081 gttgttatct atgaatctac aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa
     4141 cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt gctgatggac aaacagttac
     4201 aggtggatca attctttacc gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg
     4261 tatcggtgga gacgacactt tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa
     4321 aggccgtaac aaaaaacaag tgtctgttta tccagtagct caagaagctt aattgaatct
     4381 tatgattaaa gaggttgtga caagactttt gtcacagcct tttttgttta taaaaaaaaa
     4441 agttgtagtg gaggctacga agtttacttt gtctttttac acttaatatt tttttgccaa
     4501 tttggaatca ggatgttttg ataactatcg ttttcaattg atggaagcta tattgtttgc
     4561 tatcagtgaa ataattatat ggtaaacagt gttctttttc ttgtttccat tataaaaatc
     4621 gtatcgggat ttttcgttct tttaaattga acatttctat ctatccgcgt gttatcaaca
     4681 aaacaaaatc aggtaaatag ttgacgaaac agaaataaat acatagtatt atgttttgaa
     4741 gcctaagtac aatagtctat agaaaaggag cgataccatt ggcaaaacgc attgaccgaa
     4801 tttatatgta tgtcaaagaa aaaaccgctc atttaacaaa ggcagaatat gatcaaggag
     4861 taacaacaca ggaaattgct gaagtgttag ggattcagcg aacaaatagc agtaaagatc
     4921 tgaatcagct agtgcgcgaa ggaaaactga taaagacaga cggacgacca gtacgttaca
     4981 tatatcaaac actcgttacg cacgtaaagc cagttacaaa acatgtagtc agttacaaag
     5041 aaacgccggt ctctgaagaa aaaaatacac tccaaataga tactaaggat atctttgcaa
     5101 aaatcattgg agctaacggc agcatgaaaa attcagtgga acaggcaaaa gctgcgattt
     5161 tgtatccgcc aagaggatta aattgtttga ttacaggacc taccggatca ggaaagactt
     5221 attttgcaca cgcgatgtat cattttgcaa aagcgcatca tgtaatagat gaggaaacgg
     5281 aactgattgt tttcaattgt gctgattatg ccaataaccc tgagcttttg atgagtcatt
     5341 tatttgggta cgcaaaaggt gcttttacag gcgcagatga agaaaaaatg gggattatcg
     5401 atcaagcgga tggcggtatg ctgtttttgg atgagattca ccggttgccg cctgaaggtc
     5461 aagaaatgat cttttacttt atggatcatg gaacttacag tcgtcttggg gaaacaacga
     5521 aatcccatga agcaaatgta cggatcgtag gtgcgaccac agaagaccca ggttcttctc
     5581 tattagaaac ttttgttcgg cgtatcccaa tcaatatcaa acttccggct tttgaaaaac
     5641 gtcctgcaaa tgagaaagtc gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca
     5701 cgcagcgaaa gatttctttg acagaagatg tagcaaaagc attgatcggc agtgtgacct
     5761 atggaaatat aggacaattg aagtcaaata tccagttagt atgtgcacga ggatttctaa
     5821 atcatatgca atcacccgaa atctcgatca caatcgatga tttgacagaa gggattcgta
     5881 gcggattgat tcaattagca agcaaccgca gtgcaatggc agagctttct aaaatattgg
     5941 agccaaagat aactgtagca ccaaatgata cgatcatgaa aatccaatcg gactcctatg
     6001 agttacctta taatctatat gatatcatcg gagataaagc agctttactg aagtcggatg
     6061 gtttggatca agaagcaatc aatcatttca tttctacaga tatcaacatc catctgaagt
     6121 ctttctataa agaccatggt ttttctttta atgcagacag caaattagca gaatttgtag
     6181 atccgaaaat catagaagtg accaatcaga tttatgtaat ggtcaaaaga tctttaccat
     6241 atgagtttca gcaaaacttt atttatgcta tgagcttgca tatcagttct tttttgaaaa
     6301 aaatcaactt aggagaagaa cggcatacga atgataacat ccgtgagatg gcttttgatt
     6361 ttccagatga atatgctgtt gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag
     6421 tggagattcc agaaagtgaa gactattact tgaccgttct actcgtctcg ttacgggcaa
     6481 atcaagcaag cggcagaatc ggtgtagtcg ttgcagctca cggaaacagc acagcaagca
     6541 gtatggtcca agttgtccaa cagttgttag atgcagagaa cgtacgagca gtagacatgc
     6601 ctttggatat ggatccgaaa acagcattgg accgaattga acgcaatgta caagaagtag
     6661 atgaaggaag cggggtcata ctcttagtag atatggggtc ccttgcgtcg tttaattcgc
     6721 aattcaaaga gacactggaa ttccagtacg tactgttgat atggtaacaa cttctctagt
     6781 tttggaaact gtacgaaagg tctcagtatt agggacagat ctagatatgt tgcatgaatc
     6841 attgaaaaat tttcgaggtt acgcagaaat atcttctgta gaatctgttc aggaagcaaa
     6901 acctattcat aagaaaaaag ctgttttggc tatctgcgca tctggcgaag gtacagcaca
     6961 aagaatcaaa gaattgatcg aacgagcggt aaacaagcgt caagaaacag aattagaagt
     7021 tctagctttg tcgattgtgg aagtgaaaga agaactgcca gttatccaaa aagaatatca
     7081 gatcatagct actactggga ttactgattt gaatatccaa gccccattta ttcctttaga
     7141 acgttttatt gatcaaaata tcgaagtcat tttggatcag ttgctgatgg aatcagaatt
     7201 ggaggaaact gaattcatct ccctagatga agagtcagct aaaaatacgt gtgttgagtt
     7261 tatctctgat aacttcatct ttattaatgg tagtaagtta atcgatccaa tgtggcaatt
     7321 ttctacacaa atctctcaga cgacgggaat cggcgatgaa gaatatggat tcaaaatcaa
     7381 cctagtgatg cacacagcag gaatgatcga acgtattatt cggaatgaac cattgactgt
     7441 ggaagaaaac gaattgacaa atacaacgaa tgatcctctt tatagccaat tggcagcctc
     7501 agttgttctg ttggaagatc agataaaagt aaaagtgccg atagaagaaa tgtattatct
     7561 gctacgtcta gtccataatc agttagataa aaaagaatac actgtgccat gaatacacaa
     7621 aaacgataca cttttatagt gtatcgtttt tgtgtattgg ttaagaatcc tttaaattca
     7681 attagtgtat cgtaaaaaag ttggcacgct ttttgcttat agtagtgtgt gctaggaggt
     7741 ggttgttcac acagaaatga taggggtttc tgtgaagtag tgaggaagaa ggagttttgc
     7801 agatggatat tcgattggca cgtatagatg atcgtttaat ccacgggcaa gtcgcaacag
     7861 tttggtcgaa gcaaacaggt gttgaacgta tcattgtgat cagcgatgta gtcgctcaag
     7921 ataaattgag aaagtttcta ctaaaagaag ctgcaccgcc ggggatcaca gcaaatgtaa
     7981 taaccgtgca gaaaatgatt caaatctatc atagtggatt attatcacga gcaaaagtga
     8041 tgcttttatt caccaatccc caagatgttg aagcggttgt ccgctgcggt gtacagttga
     8101 agtcgttgaa tatcggcggt atgagtttta ctgaaggcaa atcaatgatt accaattttg
     8161 tttcagtaaa tcaacaagat attgattcct ttcgttactt gaacagtcaa gaagtggagt
     8221 tagagattcg aaaagtacct gctgacagga aaatcctttt gatggaacta ctagcgaaaa
     8281 tcaaaaaggt gtagcaagaa tggttgttgg aaaaggtcaa ataattctgt tgtaatggtt
     8341 agcaattatt aatatttttg aatttgttac aacaaaagta aataggaggt ataaatatgg
     8401 tagggattat cctagcaagt catggacaat ttgctgaagg aatcttacaa tccggttcaa
     8461 tgatctttgg cgaacaagaa aacgtcaaag ccgtgatttt gaaaccaagt gaaggtccag
     8521 atgacttgag agccaaattg gaagaagcgg ttgcttcttt tgataatcaa gacgaagtgt
     8581 tattcttagt tgatttatgg ggcggaacac cgttcaatca atcaaacaca ttattcgaag
     8641 agcacaaaga taaatgggca atcgtcagcg gtttgaattt accaatgttg attgaagcct
     8701 acgcatcacg cttttcaatg gaatctgctc atgaaattgc agcacacatc attgaaacag
     8761 caaaagacgg tgtaaaagta aaaccggaag aattagaacc ggcagaagca ccgaaagcag
     8821 cagttgaaga cgcacaacct aaaggagcac ttccagaagg aactgtggtc ggagacggaa
     8881 aaatcaaata tgtattagca cgtgttgact cacgtctgtt acatggacaa gttgcgacag
     8941 cttggacaaa agcagtgcaa ccaaaccgta ttatcgttgt ttcagatgca gtatcaaagg
     9001 atgatctgcg taagagacta atcgaacaag ctgctcctcc aggtgtaaaa gcaaatgtta
     9061 ttcctattag caaaatgatc gaagtagcaa aagaccctcg ttttggaaat accaaagcat
     9121 tgcttttatt tgaaaatcct gaagacgtat tgacagctgt tgaaggtggc gtagacatca
     9181 aagaattaaa tgttgggtca atggcgcatt cggtcggtaa agtcgttgta agtaaagtat
     9241 tgtctatggg acaagaagac gttgaagcgt ttgagaagct tgaacaaaaa ggtgtgaaat
     9301 tcgatgtacg taaagtacct aatgattcac gcgataacat ggatgatatt ttgaaaaaag
     9361 caaaagctga attagcaaaa gcgtaataaa taatattaaa atttatagga ggcttatcat
     9421 gtctattatt tcaataattt tagtcgtatt agttgccttt ctagctggta tggaaggtat
     9481 tcttgatgaa tttcaatttc atcaaccact agtggcatgt acattgatcg gtttagttac
     9541 cggtaacctc gaagctggta tcgtacttgg cggtacgctt cagatgatcg ctttaggttg
     9601 ggcaaatatc ggtgccgctg tagcaccaga tgctgcatta gcatcagtag catcagcaat
     9661 tattttagtt ttaggtggac aaggtgttaa aggtgttcct tctgcgatcg cgattgccgt
     9721 tcctttggct gttgcagggt tgttcttaac aatgatcgta cgtacaatcg ctgttccaat
     9781 cgttcatttg atggatgcgg ctgctgaaga aggaaacatc cgtaaggttg aaatgtggca
     9841 catcattgct gtatgtttac aaggggttcg tatcgcggtc ccagcagcag cgttgctatt
     9901 tatcccagca caaactgtgc aatcattcct tgaatcaatg ccagcatggt taacagatgg
     9961 tatggctatc ggtgggggaa tggtcgtagc tgtaggttac gctttggtaa tcaacatgat
    10021 ggctacaaaa gaagtatggc cattcttcgt tattggtttc gttgtagctg caatttctca
    10081 attaacattg attgctctag gtgctttagg tgttgcatta gctcttattt acttgaacct
    10141 ttctaaaatg ggtggttcat caaataatgg aggcggaagc aactctggtg accctttagg
    10201 cgacattttg aatgactatt aatcttgaag gaggaaaata aaatggcaga agaaaaaatc
    10261 aaattatcaa aaaaagatcg tttagctgtt gcgtggcgct ctaccttcat tcaaggttca
    10321 tggaactacg aacgtatgca aaacggtggc tgggcatttt ctatgattcc agcaatcaaa
    10381 aaattataca aaacaaaaga agaacgttct gcagcattaa aacgtcactt ggaattcttt
    10441 aatacacacc catacattgc ttctccaatt ttaggggtta cattggctct ggaagaagaa
    10501 cgtgcgaatg gcgcgcctgt tgatgatgta gcgattcaag gggttaaagt aggtatgatg
    10561 ggtcctttag ctggtgttgg tgacccagtg ttctggttta cagtacgtcc aatgctaggt
    10621 gcactaggtg cttcattagc tatgggagga aatatccttg gaccaatcat cttcttctta
    10681 ggttggaaca ttattcgttg ggcattcatg tggtatacac aagaatttgg ctacaaagct
    10741 ggttctaaaa ttactgatga cctttctggc ggattgttac aagacgtaac aaaaggtgct
    10801 tcaattttag ggatgttcgt attagctgcc cttgtacaaa gatgggtatc aatcaaattc
    10861 ttaccagttg tatcaactgt aaaattagat aaaggtgctt acatcgaatg ggataaattg
    10921 ccttcaggtg gtgaaggaat caagagtgca tttgaacaag ttaataatgg cttagccctt
    10981 tctcctgaaa aggtaacaac attacaagac aacttagacc aattgattcc tggtttagct
    11041 gcattaggat taacattgtt atgtatgtgg ttgcttaaga aaaaggtttc tccaatcgtt
    11101 atcatccttg gtttattcgt agtcggtgtt gttggacacg tcatcggact tttataatcc
    11161 ataaaaatat agctcgggcg ttgtcccgag ctttttttat atagtatact gttcataaac
    11221 aaatatctgg agggagaaaa tggttcaatc aatcaataca aaagtagact tggtaattga
    11281 tgctacttcg tttacgggtc tgacagatta cgggcaaatc atgataggta ataatggttt
    11341 tgagttttac catgcgcgag atagacgcaa atatatccaa attccatggg aagaagtgga
    11401 ttatgtacta gcatcagtca tgctaaaggg aaaatggatt cctcgatatg ccattcaaac
    11461 gaaaaaaaat ggtacataca cattttcttc caaagaacct aaaaaagtgc ttcgtgcgat
    11521 cagagaatat gtagatgaag atagaatggt acaatcattg agtttctttg atgtagtgaa
    11581 acgtggtatt cgtagtatgt ttaaacgcaa gtaaaaaata aagaaaactt ctaagaggag
    11641 gttttcttta tttttttact ttgcagaaga aaatatgaaa tagcaaaacc aattatttgt
    11701 cccctttaaa tcccatatgc taaaattgat tagcaaagaa ggaggttata ttatgagttg
    11761 gttatggaca ttgattgttg gagccgtgat cggagcaatt gcaggtgcga tcaccagtaa
    11821 aggaaattcg atgggctgtg taacgaatat cattgctgga ctagtcggtt caagtattgg
    11881 acaagcgatt ttcggcgaat ggggaccaaa agctgcaggt atggcactta ttccatctat
    11941 tttaggcgca gtgattttgg tagcagttgt ctcatttttc tttggaaaac gatcataagt
    12001 ttttacaaaa tagtaaacta aaaggagttg cagcgagact gtgactcctt tttggcttta
    12061 tttatttaaa ataaaaagaa gttttccgga aatttttatt taactgtcgt gtacgaagaa
    12121 aagtttgcta taatggaaaa aagcgtgcaa agaaagaggg agtttcatta tgatgctacg
    12181 tgtagaaaaa ttgcgcaaaa aaatgcaaga agaaaatttg gattcatttt tggttaccag
    12241 tccgtacaat cttcgttact taacgaactt tacaggtacg acaggattag ctgttattac
    12301 tttggaaaaa gcatttttta ttactgattt tcgttatacc gagcaggcgg cagcgcaggc
    12361 tcaagggttt gaaattatta aaaatgtcgg acctattttt gatgaggtag caaacttagt
    12421 acgcaaagaa gaactgcact cattagcttt tgaggaaaca acagtctcat ttttagaata
    12481 ttcggtttta gaagaaatca tcgatgcgga attagtacct gtttcacaga tgatcgaaga
    12541 actcagagaa gtgaaagatg aagaagaaat cgctattatc gaaaaagcat gtcatattgc
    12601 ggatatggca tatgatcaca tcctgaaaat gatccaaccg ggcatgactg agattgaagt
    12661 cgcaaatcaa ctagactttt atatgcgttc tcttggcgct tcaggtgttt cttttgatac
    12721 gatcgttgct agcggattgc gttcggctat gccgcatggt gtagcaagca aaaaaatcat
    12781 cgaacaaggt gacttgatca cattggactt tggttgctat tacgaagggt acgtttccga
    12841 tatgacacgt acattcgcta ttggtgatcc tggggaaaaa ttaaaagaaa tttatcaaat
    12901 agtcttagat gctcaattag cagttattga tgctgcaaaa ccaggaatga ctggtgtcca
    12961 attagatgca gttgctagag attatatttc aaaacatggt tacggggaag catttggaca
    13021 ttctacagga catgggatcg gtttagagat ccacgaagga ccaaacgttt cttctcgtgc
    13081 agaaaagcaa tttgtaccag gaaatgtgat cactgatgaa ccagggatct acttaccagg
    13141 aatcggtgga gtacgtattg aggatgactt gttgatcaca gaagatggaa atcgtgtgtt
    13201 gactcgttca ccaaaagaat tgatcatctt atagtttcat caatagaaga agtaagcgta
    13261 aaaatcagtg ttcacagact ttgttataga aaatcactcg atttaatgat acactataga
    13321 agaactttta agctgtaaga gataataaag ttgggtgttt tctccaattc atagcagttt
    13381 gcagctgatc ggcggcagtt ttcttaccac gtgatcacta tgccgcatca tagagaattc
    13441 ggatgtatct cgtgcttagg caaagggagg aataaataaa tggcagatga aaaaaatttg
    13501 attttaaacg caaaccaaga tttaggagag atcgtgatcg ctcctgaagt catcgaagta
    13561 attatcggga ttgcagcttc taaagtagaa ggcgtatacg gaatgcgcgg aacttttgcc
    13621 agcaatgtca ctgaattatt aggacgtgca gctcacggaa aaggtgttta cttagcaaac
    13681 gaggaagatg gcttgaaagt agatctttac tgttatctag aatatggtgt ttcagttcct
    13741 aaagtagcaa tggaaatgca ggaacgggtc agccagcaag tccttttcat gacggatatc
    13801 gatttatctg aagtcaatat ccatgtagta gctgttgttc ctgaaaaatt agaacaacca
    13861 acaatcgaag aactttttga agacgaagag gaagagaatg aataaagaat tatcaagaca
    13921 cgagattcgg gaaatggcat tgcaggcttt atttccatta gacttcaatg cagacctaac
    13981 aaaagaagat gcgattttca acgcaatcga attagaccac cgcgacatga tcaacgaaga
    14041 tgaatcagag tttgttcctg tttatcttga tacgctagtt ggcggtgtct gtgcaaaaaa
    14101 agatgaattg gataaagtga ttgaaaaaca tttaaaaaac aattggcaca tcaatcggat
    14161 ctcaaaaatg gatttggtga tcttgcgtat cgcgattttt gaaatgactt atgtgacaga
    14221 tgtaccagcg gctgttgctt taaatgaagc aatcgagttg gcaaaaacat ttagtgatga
    14281 ccgttcacgt aaattcgtca acggtgtttt atccaatgta ttgaaagaat tagaagctgg
    14341 ggcgtaagtc ctagcttttt tctatgaatc atcataaacc tgttataaat cgggaaaatt
    14401 ctatggtaga atgaaaacac aaaaaatatc tgggagtggt tagatggcag aattgattaa
    14461 cggaaaagaa ttagctgaaa aaatgcaggc agagattgct gaaaaaatcc aaaaattgaa
    14521 agataatggt atccatcccg gattagtcgt tttattggta ggggaaaatc cagccagcca
    14581 aatctatgta agaaataaag aacgtgtagc aaaagaaatc ggaatccatt cattggtaga
    14641 gcgttatcct gagacgattt ctgaagacga actacttgca gaaattgaaa aatacaatca
    14701 agatcctagt tttcatggta tattggtcca attaccattg ccaaagcata tagatgaaga
    14761 aaagatcctg ttagctatcc gccctgaaaa agacgtagac ggttttcatc cgatgaattt
    14821 aggcagactc cttgcgggta aaccggacaa gattccttgt actccatacg gcatcatgaa
    14881 aatgtttgaa gcttattcta tcgatccggc gggtaaacga gcagtagtta tcggacgaag
    14941 caatatcgtc ggtaaaccga tggctcagct actcttgatg gcagatgcaa ctgttactgt
    15001 tgctcattct aaaaccgaaa atctggctga actggcacaa gaagcagata ttttagtcgt
    15061 agcaatagga cgtggacatt ttgtcacaaa agaattcgta aaaccaggtg ctgtagtgat
    15121 agatgtaggg atgaatcgtg atgctaacgg taagctgatc ggtgatgtga aatttgatga
    15181 agtggagcct attgcaggct ggatcactcc tgtgccaaag ggtgtagggc caatgactat
    15241 cacgatgctg atgtatcaaa ccgtaaaatc tgccgaagct tttggagcag gtgaatagat
    15301 ggcacaagaa tatttaactg tcactacatt aacgaagtat ttgaaaagaa aatttgatgc
    15361 cgatccttat ttagaacgcg tatatttaac aggcgaaata tcgaatttcc gtttacgtgc
    15421 caatgcccat caatatttca gcttaaaaga tgaccatgca aaaatatctg ctattatgtt
    15481 caaaggagca ttccaaaaat tacgattcca gccaaaagaa ggaatgaaag tccttgtcgt
    15541 tgggcgtatt tcattatatg aagcgggagg ttcttatcaa atctatgtag agcacatgga
    15601 gcctgatgga ataggtgctt tatatcaaga attggaagaa cgaaaagaaa agctgatgaa
    15661 ggaaggcctt tttcaagggc ctaaaaaaat gctgccacgt ttcccaaagc gaatcgcggt
    15721 ccttaccagt ccaagcggag cggttatccg cgatatcatc acgacggtga aaagaagata
    15781 tccaattgtc caattggttt tgttcccaac agttgtacaa ggaaaccaag cagcggacaa
    15841 tgttgtcagg aatatccaga gagtagaaga gatgggcaat ttcgatacaa tgatcatcgg
    15901 tcgtgggggt ggttcgattg aggatttatg gccgtttaat gaagagcggg ttgctcgggc
    15961 gatttatcaa gctaatacgc cggtgatttc atctgtagga catgaaacag ataccacgat
    16021 cgctgattta gtagcagatg ttcgggctgc cactccaaca gcagctgcag aattagctgt
    16081 tcctgttttg tcagaagagt tgatgcgcat tcaagaacgg caagctagat tgcagcaggc
    16141 gtttgtacgc cagatccagc gcaaacaaga acggttcgag cgtgctcgac gttcctatgt
    16201 atttcggcag cctgaaagat tgtatgaagg tcagaccatc aaattagacc agttgaaaca
    16261 gcgattatat caagctacac agcaaattta tcatgcgaaa gaaaaacaaa cgatcgcatt
    16321 aagccaccaa ttggaacaag tggctccgct ttatcgagtt aaagcagctc gccaagaaac
    16381 tgcttatcta gaaaaacgct tagaagagaa aatccaacaa tatatgaaac aacagcagca
    16441 gcgttttgct caagctgttc aatctcttga tttgttgagt ccattgaaaa tcatgggaag
    16501 aggctacagc tatacgacgt taaatgatca agtagtaaaa agtgtcaccg agattcatca
    16561 aggggatttg ctgacgatcc attatcaaga tggaatcgtt aaaggacaag tacaagagat
    16621 agacaaagga gaataaaaat atggaaaatc caacttttga agagtcactg caagaattag
    16681 aaaaaattgt catgcagtta gaacaaggag acgttccatt agaatcagct ctagattcgt
    16741 ttaaacgagg aatggaatta agcaaacatt gccaagatac attagctaaa gcagaagaga
    16801 ccttgacgaa aatgatgaca gaatcaaatg aagaagttga ttttgacgga aacgaggaaa
    16861 gctgagatga gattaactga cttttcagct attcatctac ctgctgtaga aaaagaaatc
    16921 ttgtcttttt tagatgagca tacaacggat agaggactct atgatgcgat gagttattca
    16981 gtgaaagcag gcgggaaaag aatacgtccc ttattacttt taacggccgt tgcttccttt
    17041 gatgagccta ttgatgttcc cgtctatcaa gtagcagctg ctttagaaat ggtgcatacg
    17101 tattccttga tccacgatga tttgcctgcg atggacaatg atgatcttcg tagaggaaaa
    17161 cctaccaatc ataaagtatt cggcgaagca ttagctatat tggccggaga tggtttgctt
    17221 acaggagcat tccagctaat cagtatggca cacttaggta attctcctaa gcttctgctg
    17281 cttcagcaat tagctgtttg tgcgggatca caaggtatgg ttgctgggca agctgcagat
    17341 atcgagggcg aatcgaaaaa gctttcttta gaagaattag catttattca tgaaagaaag
    17401 acagggcatt tgattcgtta tgccttgctg gctggtggca tattagcgaa gcaacctgaa
    17461 gaaatcttat tgctacttca acgattagcc gaacatttag gtttagcttt tcagataaga
    17521 gatgatctac tcgatgtgat tggtacaacg aaaacattag ggaaaacagc tggtaaagat
    17581 gaacgtatgg aaaaaaatac ttacccgcgt ttattaggcc tagaaaagac acgtgaggcg
    17641 ttggagattg agctgttatc cgccaacaag atcatagaca aattagaaga ggacgttttt
    17701 tcttttgatg gcgggctgat gcgccagatg atcaagcaat ttgatgtgga gaaatgaggg
    17761 aaagaatgga aaaagaacgc gtagatgtat tagcggtcaa acaaggatta tttgaaacaa
    17821 gagaacaagc caaacgttca gtgatggctg gtttgattta caacgaaaaa aacgagcggt
    17881 ttgataaacc gggagaaaaa attccagtga gtagtgaatt aagagtcaaa ggtaaaaaac
    17941 ttccttatgt ttctcgaggg ggattaaaat tagaaaaagc actcaaagca tttgatctat
    18001 ctgttgaagg aaaaacattg ttagatattg gtgcatccac tggcggattt actgatgcag
    18061 ctttgcaaaa tggcgcaaaa atgagttatg ctttagacgt tggctacaat cagctagctt
    18121 ggaaaataag acaagatcct agagtcgtcg taatggaaag agtgaatttt cgttatgcaa
    18181 aacccgaaga ctttacctta ggaattccag aagttgctgt tatcgatgtt tcttttattt
    18241 cactaaaact gatgctgcct ccattacatg ctattttaaa agaagaggga gaagtgattg
    18301 ctttaatcaa accccaattc gaagcaggca gagaagctgt agggaaaaac ggaattgttc
    18361 gtgaccctca gacccatcaa aaagttttag aagacatttt gtcatttgcg gcacaaggtg
    18421 gatatgatgt gctggaatta agttattctc cgatcacggg gggagaagga aatatcgagt
    18481 tcttggctca cttgaggaaa gtacccgaaa gcggaacaat caattcagct attaatatgg
    18541 cagaagtggt ttcaaatgca cacgaacagt ttgaccataa ataaattgta actgtgaaaa
    18601 tcaagtgaga agtatgcgga ttcttcttta ttataccaag gaaataagct atcattgatt
    18661 aagaagaata tgctaaggag cgaagacgta atgagaaaaa aagatcggca tcgtttgatt
    18721 actcgattgt tgagtgagca agatatccga aagcaggaag aatttgttga aatcctgaaa
    18781 agcaaaggaa tatctgttac tcaagctacg atttccagag atataaagga actgaaactg
    18841 atcaaggtac cggcttcaga tggcggctac cgttatagca taccagctga aacaaacgaa
    18901 gatgtaaata ctaaattgga aaaactatta aaagacgctt tcgtggcagt tgaccaaatg
    18961 gaaaaatttg ttattttgaa aacattgccg ggaaatgctt cggcagcagc aaatttgatt
    19021 gataaacgtt acaagaaaga attattttcg attatcaatg atgacgataa tgttttgatg
    19081 attacacgaa ctgaagacca ggcagtttta ttgaagaaag acttcctgca ttacctttaa
    19141 gttgaggagc gaatgtgatg ctacaagaaa tcagtattac taactttgcg attattccgg
    19201 aattgcggct ttcttttcac gaaggaatga ccgcgctgac cggagagact ggtgcgggta
    19261 aatccattat tatcgatgct ttaggtctgt tagcaggagg aagaggttca agcgattata
    19321 tacgtcaagg agcagaaaaa tgtgtgcttg aagggttgtt tgaactacca aaacaagaag
    19381 gattctctga actgatggtt gaattaggga tcgaaacaga tgaggacaat ctgattgttc
    19441 gacgggatat gtccttaact ggaaaaaatg tttgtcgagt gaatggtcat attatcacat
    19501 tagctaatct gagaagaatc ggcagttatt tagtagatat ccaaggacaa aacgaacatc
    19561 aggaactgct ccagcctgaa tcacatcttg ctttattgga tcgttttgga gatgtagctt
    19621 tccaacagaa gaaaaagagt tatcagcaag aatatatctc ttatcgtgag ctggaaaaaa
    19681 gggtacgaaa agtgcagcaa aatgaaaaat cctatgttca gagaatagat atgctccatt
    19741 tccagcaaga agaaatagct gctgctgatt tgcaagtcgg agaagaagag aaattaaaag
    19801 aagaaagaga aaaacttagc aattatcaaa aaattgctga tggattagct gcaggttacg
    19861 gagcactgac gaataatgaa caaaacagtg tagatggtgt tggcttggcc gtttcagaac
    19921 tgcaagggat cgcccatcta gatgtggagt atgaagcaat ttatgaaaat atccaaagtg
    19981 cttactattt gctgcaagat gcaatcggcg atatgagtcg tcagattgat ttattggagt
    20041 tagatgagag tcgtttagaa gaagttacgc aacgtttaga attaatccga cagttgaaac
    20101 gtaaatacgg cgaatccatt gagtcaatat tggcttacta tgacgaaatc accgaagagc
    20161 tagcttcttc tgatttttca gaaggtcaat tggacaaaat gaaagaggaa ctagagcaaa
    20221 aagagcttct acttcagcaa caagccgcag atcttcatga agcgcgtaaa aaaatcgcaa
    20281 aagaattgga aaaatccatt ctgcatgaat tgaaaagtct gtatatggaa aacactgagt
    20341 ttgaggtacg tttcttaaaa gaagaaaata gacaattgaa tcatgatgga tttgaccaaa
    20401 tcgaatttta cattacgacc aatccaggag agccactgaa gcctttggtg aaagtcgctt
    20461 ctggtggcga actgtctcgg atgttgcttg ctttgaaaac tattttttct tctgagcaag
    20521 gtgtgacaag tattattttt gatgaagtgg atacaggtgt gagcggaaga gttgcacagg
    20581 ccatcgcaga taaaatttct caaatatcga aatactctca agttttgtgc atcactcatc
    20641 tgcctcaagt tgctgcagtc gccgactatc aatattatat tgtgaaagca gtgattgatg
    20701 gacgtacgca aacttctgtt tcagagttga aaacaaaaga aagagaagaa gaaattgcac
    20761 gtatgctggc tggaagtgag attacaaaac tcacactcga acatgcaaaa gaactattga
    20821 aactcgcaaa aaggacctga gccacctcgc agtggttcag gtccttttca gcagctgata
    20881 gctatatttt atttttacat gacactcatt acagcagtca atacaacaga tccaatcgtt
    20941 aaaatcagca ttgtccaaat gacaaatttt gttactttag caaaagtact tgtttgtttt
    21001 ttttcacgca ttgttttcaa cctcgtcatt aattagttga ttcaagtgta caatcttttt
    21061 tccatttcca caagagggct tttttataaa aacttccctt tttttaaata ataggtagtt
    21121 acgatacaaa taaataatgt taatagaact agccatataa aggcatcatc cctttcagca
    21181 ataggtaact taatgttcat tccatagata ctgccgacga ttgtcggtat agttaaaata
    21241 atcgttaaag aagttaaaat tttcataata ttattcagat tattcgatac gattgctgaa
    21301 aaagtctgat tcatttgttc tactagtttt aattgaatgc gagaagtagt cattgcctgc
    21361 tctgtttcca ccaatacatc acgtaaatga tttttcaaag catgattttc ttggaaaagt
    21421 ttattttgcg ctagttcttt caatgcatct aaattagcag aagtcgcttc tctgaaataa
    21481 acgaggcttt tttggatatc catgatttga taaagttgtt tgttttcagt ggatacttga
    21541 agtttggctt ccaaagtatc gcattgagat tgaagcgtag ctaaataact gttgtaagag
    21601 agtgtgacct gccaaatgaa ttgaaagaag atattgagat taggcgaaat atcacttttt
    21661 ggaaaggtct ggctaaagac tttctttaaa aacagtggtt catgattcgt aattgtgata
    21721 agcttctttt tgggcgtcaa aataatagag agcgggtatg tatccacttg aaaataaccg
    21781 ctaggacttg ttttagcaaa aggatattgt aacaataata acgctggctt tgtcaacttt
    21841 gattgattga attcttctgt acgggaattt tctgcatcgt ccaatatgga agtgatgtaa
    21901 tctgacggaa tttcaaattg atgtgtcagc tgatgaatct cttcttgatt aggacgttct
    21961 acacaatacc agatcgtttg gtcagtttcc tttccggtat tacgcagttt ctcattatct
    22021 aatgtaaaat atttgatcat gattttcctc ctgttgcctg tttcttttat tataacggat
    22081 agggaattgg aatacatgga atgtgtttgt aagatataga atataactgc ataaatttaa
    22141 ataaaatgaa acagttgtgt aaagtatata cagtatactt gaagcaacga taaaaaagga
    22201 gtttacgttc atgggcacat tcacaccact tatcttgttt tggtatattt acccggtaat
    22261 cgttttgttt ggttgccaat ttcttgtaaa attattttcg ttaaatcgtc gttttaaaat
    22321 aaaagcccct gatttggcga ttcctttttt gtggggcggt atgcatgcat tgtctagaaa
    22381 tacagggaca atttctatct taccgttttt ctttatttct gttttgctaa tgggaatttg
    22441 tattgctgtt ttccaagcat attattatga agaaattatt tatccgcgtt atttcaagat
    22501 gacttggcgt ttggtttttc tgttgacaat cgtcttgtac gctgtcttga tcattgtgaa
    22561 catactttct tacctatgaa aaaaatagaa aagctgtaaa aagtctttta aaaggctttt
    22621 tgcagcttga tttttaaaaa aatgtttatg aataaaaaaa gttgttcccg gatttcccag
    22681 aaaaggtggt agaaagtggg ggattgtggt agactaattt tagttagtgg aggaatgggg
    22741 ggctttcaat gttgatgggt gaattccaac ataatattga tgcaaaagga cgtttaatcg
    22801 tgccctcaaa acttcgtgaa gaacttggcg aaaagtttgt tctgacacgc ggattggatg
    22861 gctgtctttt cggctatccg atgagcgaat gggaaaatct ggaagctaaa ctgaacgaaa
    22921 tgccgttggc taaaaaagac gcacgtactt ttgtgcgatt cttctattcg gctgccactg
    22981 aatgcgaact tgataaacaa ggacgaatca atatacccag cacgttaaga aattatgcag
    23041 ctttgacaaa aggttgcgta gtcatcggtg tatctaaccg aattgaaatt tgggatgaga
    23101 cacgatggca agatttttct gcagcagcag aagagaactt tgacgaaatt gctgaaagta
    23161 tgattgattt cggactataa gaataggaag aggctgacta atgactgaaa catttcaaca
    23221 ctatacagtc atgttgaaag aaacggtaga tggattgaac atcaaaccgg atggcgttta
    23281 tgtagactgt acactcggcg gtgccggaca tagtgaatat ttattaagcc aactaagttc
    23341 taaagggcat ctgtatgcgt ttgaccaaga tcaaaaagca atcgatcacg ccaagctgcg
    23401 tttagcttct tatgtcgaac aaggtatggt cacatttatc aaagcgaact tcagagaatt
    23461 agaagaacgc ttgagtgagc atgtagataa ggtagacggc attttgtatg atctaggggt
    23521 ttcctctcct cagctagatg aagcagaacg tggatttagt tatcatcaag atgcaccgtt
    23581 ggatatgcgc atggatcagt cagctccttt atccgcttat cacgtaatta atgaatattc
    23641 ctaccatgaa ttagtaaaga ttttcttccg ctatggcgag gagaaatttt ccaaacaaat
    23701 tgctcgtgaa atcgaacgag tacgaaaaac aaaaccaatc caaacaactg gtgagcttgt
    23761 cgaaatcatt aaacaagtca tcccagctcc cgcaagaaga aaaggcgggc atcctgcaaa
    23821 aagaattttt caagctgtgc ggattgcagt aaatgacgaa ctcggtgccg aagaggcctc
    23881 tttagaacag gcaatccggt tattgaaagt caatggaaga atcagcgtaa tcacattcca
    23941 ttcattggaa gacagaattg tcaaaagtat gtttaaagaa tatagtacgg tacaagatct
    24001 accaccggga cttcctgtca tacctgacga attccaacca gaactgaaag tcattactcg
    24061 taaaccaatc cttccttcag aggaagagct aacagaaaac aatcgatcac gcagtgcaaa
    24121 gttacgtatt gcagaaaaaa taagaatgaa agaggagtag aatgatggca gaattaaaga
    24181 aaatgcaaga ttacgaatat gatttacctg aattggtgga tcagccggaa caacaaatgc
    24241 ctgattcttc ttcgaatcaa gaaatcattc ttccacaatc accggcaaaa cgattaaagc
    24301 agatttctgc tttggaaaaa attgctgtgg caagcgtggc gattgctatt gttgctttat
    24361 gtgttttaac agttatgctg cgcacaaata tcagcggagc tgaaaaagaa atcacgtcaa
    24421 tacaaacaga aatcaatgat aaaaagcaag aaaaaacgag tttagaacaa gaaaaaaacg
    24481 aactttctcg tactgaacgg attaaacaaa tcgcagaaga aaaaggattg tctattaatg
    24541 acgacaattt aaggaaagtg aagtaaatga gtcttaaaaa taaaattcgg cggtttatgg
    24601 aaaagaaaaa tctaaatccg atgaacaatc gcaaaaaagt aggtatcatt ttatttgcta
    24661 cgagtattgg attgttcttt ttatttgccg tccgtttctc gtatatcgtt attggaggtc
    24721 atgttgctgg tacctcatta gctgaaaaaa caaaacagtt gtaccaagga agtgaagtag
    24781 taaaagcaaa aagaggaacg atttatgatc gtaacggtgt ggcgctcgca gaagatgcct
    24841 cctcttattc aattaaagcg atactctcaa aaacatatac ctctggggat aaaaaactgt
    24901 atgttgaaga aaagaatttt gataaaatag cagaaatcct gcataagaat ctttctatag
    24961 acaaaaaaga tgcactgaat attttagagg acggcgcaaa aaaagagctg tatcaagttg
    25021 aatttggtag ctatgggaaa aatatctccc aagaaaccaa gcaaaacatc gaagcggaca
    25081 tgaaaaaaga aggtgtcgct ggtctttatt ttgtcgacca tcaggctcgg atgtatccaa
    25141 atggcgtctt ttcttctcat tttattggat acgcagttcc agacaaagat gagaatggct
    25201 tagttggtaa actaggtttg gagtctgctt acaatgatat cttgagcggt aaagatggta
    25261 agattattta tcaaaaagat aattttcaaa atcctctgcc aggtacagtg gcagaagaag
    25321 aaaaagctgt cgacggacaa gatatctaca cgactttaga cagcagacta caaagttatt
    25381 tagaaacact gatggatcaa gtaaatgaag aatatcagcc agaagaattg acagcagtcc
    25441 tgatgaaagc aaaaaccgga gaaatcttgg caatgggaca acgacctact ttcaatcctg
    25501 aaacaatgga aggattgact ggaaaagatg ctatctggcg taatttcttg gtacaagata
    25561 gttatgaacc aggatcaacc atgaaagttt tcactactgc agctgctatc gaagaaggag
    25621 aattcaacga gaatgaaacc ttccaatctg gaaaaatcca agtagaagat gctacgatca
    25681 atgaccatga ctttggtgaa aaaggtgtgc tgaccatgcg tcaagctctt tcatggtcaa
    25741 gtaacgtcgg gatggtcata ctggaacagc gattaggcgg acgatggtat aactatttac
    25801 aaaaactggg atttggacaa agtactcatt ctggattaga tgatgaagta aacggtgctt
    25861 tacctacatt aaatattgtc gaccgagcaa tgagtgctta tggacaagcg gtaggtgtga
    25921 cgaatttcca aatgatgaaa gctttcactt ctattgcgaa taacggtaca atgattcaac
    25981 ctcgatatat cagcaaagtg gtggatcccc aaactggaga agagcgaaca actcaaacag
    26041 aagttctagg gcaacctttt tcaaaagaaa caacagaaaa agtccgtgaa tatatgagag
    26101 atgttgtcga aagcgaaaac tacggtagtg cttatggtgt atatagtgta cctggataca
    26161 atgtatcggc aaaaaacgga acagcccaaa tcgcttctga tacaggggga tatcaaacag
    26221 gagatacagc ctacctgtat tcgattgtgg agatggttcc ttctgaagat ccagactatg
    26281 tcttgtatct aacaatgaaa caccctaaga catatgatcg aatggcattg gctaaaatcg
    26341 caaatccatt gatgaaacgg gcaatggatt ttaaagaaac tgaagaagat tcagatacag
    26401 aaacaaaaac agaaaaagta tccgtagccg attatcgtaa tcttgaagcg gacgttgcag
    26461 cagctgatgc tcaaaaaagt ggactgcaac ctgtagtgat cggtaatggt aaaaaagtac
    26521 aaaaacaatc tactgccaac ggtgatcagt tgatctctgg agaaaaactg atcctatata
    26581 ctggtggtga taagctgatg cctgacgtaa caggatggtc aaaagcagat atcatgaagc
    26641 tggggaaaat cctcggtatt gaagttagtt ttgatggaga tggttattgt gtcaaacaag
    26701 aacttgctcc atatgaaaaa atcactgaag acaaacttag ttttacttta gaagaataag
    26761 aagagaaata ggagagaaaa gagcatggaa tggacacaag cattgattcc cattgtcagc
    26821 agttgtgcaa tgacaattgc cgcaatgccg ctatttattg gttattttca aatgaaaaag
    26881 caaggtcagg cgattcgtga ggagggacca aagtggcaca atagcaaagc cggaactcct
    26941 acaatgggcg gcttagtgtt tctgatcggc tctattttga caggaatttg ggttggagct
    27001 tggcaaaaac agctaactcc tactcttttc atcttgcttt ttgtattagc gttatacgga
    27061 gtaatcggct ttcttgatga ttttataaaa atattcaaga aaagaaacat gggactgaac
    27121 tcgaagcaga aattgattgg acagattcta gggggaatca tcttttatct tgtttaccgt
    27181 tctgaaggtt atccaggtgt attgaatttc ttcggactag atctaccttt aggaatcatt
    27241 tatggtattt tcgctctctt ctggcttgtt ggattttcaa atgcagtaaa tctgacagat
    27301 ggcatcgatg gattagttgc tggcttagga agtatctctt ttgcagctta cggtctaatt
    27361 gcttggcatc agcatcagta tgatgtatta atcatttgct taagtgtttt aggaggttta
    27421 ctgggtttct tcgtctacaa cagaaaacct gccaagattt ttatgggaga tgtcggttca
    27481 ttagcgttgg gcggactttt agcggcaatt tctataatgc taaaccaaga atggacattg
    27541 cttttgattg gtttgatcta tgttatggaa acagcaagtg tgatgcttca agttacttcc
    27601 tttaagctta caggaaaacg aatttttaaa atgtctccaa ttcatcatca ctttgaaatg
    27661 tgcggatggt cggagtggaa gattgataca gttttttggt tgatcagcat tgtgacttcg
    27721 ttgatcacat tatggatcgt ttggtaggag atgacgggaa atgaaaaaaa tatcaacata
    27781 tgaaaataag aaagtgcttg tcttaggact cgcaaaaagc ggagtaagtg cggccaaatt
    27841 attacatgaa ttgggcgcac ttgtcaccgt taatgatgga aaaccttttg acgaaaatcc
    27901 ggaagcacaa gagctgcttt ctttagggat aaaagtaata acaggtagtc atccaatcga
    27961 attgttggat gaagaatttt cattgatggt caaaaatcca gggattccct acagtcatcc
    28021 tttagtagca aaagcccaag aaatgggtat tccggtaatt acggaagttg agctagctta
    28081 cgaagtagcg gaatgcccaa ttattgggat tacaggaacg aacggaaaaa caacgacaac
    28141 aacgatgaca ggactactac tgaatgcagg agcagatcaa ggaattgcac gtttagcggg
    28201 taatattggg tatccagcaa gtggtgtagc gcaagaagca aaatcagaag ataaaattgt
    28261 gatggaattg tctagcttcc aactcatggg aatcacagat ttccgccctc atatagcggt
    28321 gattaccaat atctatgaag cacatattga ttatcatggt acaagaaaag aatatgtcaa
    28381 agctaaatgg aaccttcaaa aaaacatgac agaaaaagat tatctgatcc ttaactggaa
    28441 tcagtcagaa ctacaagaat tagcgcaaag aacaaaagca cgcgtactgc ctttctcgac
    28501 aaaagaagtg ttggaagacg gtgtgtatgc tgacgattac tcaatatact ataaaaaaga
    28561 aaaaataatg gaaatatctg aacttggcgt accaggtaag cataatgtag aaaatgcact
    28621 agctgctatt tctgtagcaa aattgtatgg tatatcaaat gaagctatac gagaaacgtt
    28681 gcattttttc catggcgtac cacatcgtac gcaatatgta ggagaaatcc aaggcagaaa
    28741 attttataat gattcaaaag caaccaatat tttagcgact aaaatggctc taagtggatt
    28801 tgaaacgtct aaggtcgtgt tattggctgg cggattagac agaggaaata catttgatga
    28861 attaattcct tcattaaaag gaatcaaagc aatggtcgta tttggccaaa caaaagaaaa
    28921 gttgatggat gctggaaaaa aagcaggcat cgagacaata gtaacagccg attctgtaga
    28981 acaagcagtt cctttagcat tagaaaacag tacagatggt gatgtagttt tattatcacc
    29041 agcgaatgcc agctgggatc aatacccaaa ttttgaaact agaggaaaca gattcatgga
    29101 agctgtgaac cgattaaaat agaaaaagta gaagcgagcg ataaaaaatg aaaattttgg
    29161 ttacaggcgg aggaacaggg ggacatattt atccagctct tgcctttgtc aattatgtaa
    29221 agactcaaga accgaatgct gaattcatgt atgtgggagc aaaacgaggt ctagaaaaca
    29281 aaatcgtccc tgatacagga atgcctttcc ataccttgga gattcaagga tttaaacgaa
    29341 aaatttccat gcataacatc aagacggtcc gattgtttct taagagtatc cgtcaagcaa
    29401 aaaaaatcct cagtgatttt caaccagata tcgttatcgg tactggagga tatgtttccg
    29461 gagcggtagt gtacgctgca gccaaaatgg gtattccaac agttatccat gaacaaaaca
    29521 gtgtaccagg tgttaccaat aaatttttaa gccgatatgt ggatcggatc gctctttcat
    29581 ttaaagatgc ggcttccttt tttcctgaaa acaaagcagt tcttgtaggt aatcctcgag
    29641 cacaagaagt agcagatact aaaaaatcag atattttgaa aacatttgat ttggatccag
    29701 aaaagaaaac agtcttgatt tttggcggta gtcaaggagc acttaaaatc aatcaagcag
    29761 tatcagtttt tttaagtgaa tatattcctt cttcttacca agttttgtat gcttcaggag
    29821 atagatatta tgaagagatc ctaaataaaa taaatcctga aatacaaaat attagtgtcc
    29881 agccttatat caaaaatatg gcagaggtca tggcttccag tgatctgctg gtggggcgtg
    29941 ctggtgcaac ttcgatcgca gaattcacag ctctggggtt accggcagtg ttggttccca
    30001 gcccatatgt tacgaatgat catcagacta aaaatgcaat gagtcttgtt cacgcaggag
    30061 cagcaaaaat gattgcagat aatgaactaa caggtgaatc actaagtcag acagtcaacg
    30121 aaatcatggg tgatgaagaa ttgcaaaagc aaatgtgccg agcttctaaa gaacaaggaa
    30181 tccctgatgc ttcaaaacgt ctttatgact tagtaaaaca aataatcaaa aaataaaggc
    30241 ggtggtctta gtgagtaaga aaaaagaacc aaaaaaattg tctcatccgt cttctgatga
    30301 aaacaatttg actccttggc aaaaggctaa ccgaaaatat ctggcagaac aagagatgaa
    30361 aaaccaagag ccaaaaaaag aagacataac attagataca cccctttcag attctgaaga
    30421 agaaaaaacg gttcaagagg aacagcaaga agaaagccag tccgagaaaa aaatcgaatt
    30481 ttttgaagag atcaagcaaa agaaaaacgg tggaccatat aacggttctt ttttgaaccg
    30541 gctgcccaat ttaaaaagtc aacggcacaa agtactattt cgtcggttag ctttgatcat
    30601 cactgtattg ggtataccgc tgattttttt gatctattat gtttctcctt atagcaaatt
    30661 acaagcagtt gcagtatcag gcaataagac agtcaacagc caagaaatca tctctgatac
    30721 aaaattgtca ttaggagaaa atgtttgggg acagtatttt catcgatcta cttatatcga
    30781 tcgccttaaa aaagcacagc caagaatcga aacagcaaat atacatttca aagggatgaa
    30841 tgaattcgat ttggatgtaa cagaatataa agagattgct ttgatcgcaa aaaacaatca
    30901 atactaccca gtgattgaaa atggaacagt attagatgaa aaagtagcaa atcctacaaa
    30961 aaatctccct atcttggaag agttcaaaga ctctgcaaaa atcaaagaat taacaaagca
    31021 atacaatcag ttatcatcag aattacaaaa agcaatttct gaaatcaagt acacgccaag
    31081 agcaagcaat aaaaatctga tccaattgaa tatgaatgac ggcaatcaag ttattgtaaa
    31141 tatcaataat cttgcgaacc aaatgaaata ttattcgcaa gtagccaaag atatggatga
    31201 aaaaggtgtg atcgacatgg aagttggtat cttctcgcat ccttacggta gcactcaaga
    31261 aacggaagaa tcgggccaat cggaagaaac aactgaaagt gaagaaaatg cggatcaaac
    31321 aaacgaatca agtgaagagg cagaaacgca aaaagcacaa acacctggat cgtctagcac
    31381 aaacggatca tctgattcaa atgattcaag tgaaacaagt caaatagaag ataatccaag
    31441 tacaactccg tcaagtttaa atgaaaattg ataaaacaac tttaaaaagt aagaatgtac
    31501 tttctaaaaa ccagtaattt atggtagaat tggaagcagt gaatatatct attaataagt
    31561 atattttaaa acggacgcta gttgttcgct tgtatatgga atagtaagta ggagggaatc
    31621 ccattcatgg caaaaacagg aatgtatgta ggccttgata ttggaacgac atctgttaaa
    31681 gttgtcgtgg ctgaatatat cgacagccaa atgaatatta ttggagtagg aaacgcaaaa
    31741 tcagaaggga ttaaccgagg cattatcgtt gatatcgata aaacggtcca agcgatacaa
    31801 cgagcagtac gacaagcaga agaaaaagca ggtatccaga tcaaaggcgt aagtgtcggt
    31861 ttgccagcaa acatgcttga agtagaaaat tgccagggaa tgattgcagt taacggagat
    31921 tcaaaggaaa tcaccgatga agatgtcaga aatgtggctt cagctgccct tgttcgttca
    31981 atacctcctg aacgtcaaat cgtgtcaata ttgccacaag attttactgt cgatgggttc
    32041 gagggaatca aagatcctcg tggaatgatc ggcgtaagat tagaaatgta cggtctgttg
    32101 tttacaggac ctaaaacgat catccataac atacgtaaat gcgtggaaaa tgccggatta
    32161 atcgtcaacg aaatggtgat tactccttta gcgctgactg aatcaattct ttctgacgga
    32221 gaaaaagatt ttggtacgat cgtcattgat atgggtggcg gccaaacaac aacggctgtt
    32281 atgcatgata agcaattgaa gtttaccaat cttgaccaag aaggtggcga atttgtcact
    32341 aaagatatct ctattgtatt aaatacgtca tttaacaatg cagaagcgtt gaaaataaac
    32401 tacggggatg catacccaga acggacatca ccagatgaag aatttccagt agatgtgatt
    32461 ggtcagtcag aaccagtgaa agtcgatgaa cgctacttgt cagaaattat ttcagctcga
    32521 atggaacaga ttttcaacaa agcaaaagat gcattggacc aaatcgaagc attagagttg
    32581 ccaggtggcg tcgtattgac aggcggagct gctagtcttc ctggtgttgt ggacttggca
    32641 caagaaatct tcggcgtaaa tgtaaaattg tatgttccaa atcacatggg gctgagaaac
    32701 ccggtattta caaatgtaat aagtatcgtg gattattcag caaacttgag tgaagtgtat
    32761 caattggcaa aaattgctgt tacgggtgaa acctcagcag ctcgtcaaat ggtagttgaa
    32821 caagaaacaa caaacacata tgagagctat gaagcacctg aagaaacaat ctacgatgag
    32881 ccggaaccaa aagagtccgg agaaaatgta aaaaataaaa tcaagggttt ctttacaaat
    32941 atttttgatt aattcatcct gggaggaata agatatggaa ttctcaattg ataataatat
    33001 caatgacggc gcagtaatca aagtcatcgg agtcggtggt ggcggtggaa atgctgtaaa
    33061 ccgaatgatt gaagaaaacg ttaaaggtgt tgaattcatt acagccaaca cagacgtgca
    33121 agcattaaaa aattcaaaag ctgaaacagt tatccaatta ggccccaaat atactcgtgg
    33181 tttaggtgct ggttctcagc cagaagttgg acaaaaagcg gcagaagaaa gtgaacaatc
    33241 cttacgcgaa gctttagacg gtgcagatat gattttcatc actgcaggaa tgggtggagg
    33301 aaccggtaca ggtgctgcac caatcgttgc aggtatcgcg aaagaattgg gcgcattgac
    33361 tgttggtgtc gtgacacgtc cattcacttt tgaaggaccg aaacgtggcc gttttgctgc
    33421 agaaggtatc gctcgtttga aagaaaatgt ggatacatta ttaattatct caaataaccg
    33481 tttattagaa gttgtagata aaaagacacc gatgcttgaa gctttccgtg aagcagataa
    33541 tgttttacga caaggtgtac aaggtatctc tgatttgatc acagcgccag gttacgttaa
    33601 ccttgacttt gctgacgtga aaacagtgat ggaaaaccaa ggtacagcat tgatggggat
    33661 cggtgtggct agtggagaag atcgcgtagt agaagcaacg aaaaaagcaa tctcctctcc
    33721 attgcttgaa acatctattg atggagctga acaagtactt cttaacatca caggcggttt
    33781 ggatatgaca ttgttcgaag cacaagatgc ttcagatatc gtagcaaatg cagcaactgg
    33841 cgatgtaaat atcattctag gtacatctat caatgaagaa atgggcgatg aaatccgtgt
    33901 gacggtcatc gcaacaggta tcgatgaatc aaaaaaggaa cgcaagtcta gccgaccagc
    33961 tagacaagca caaatgcaat cacctgctca aaagactgta ttagatatgg atcaagctaa
    34021 accaacttct tcagaagaag aaaacagttt tggcgattgg gatatcagac gtgaacaaaa
    34081 tgttcgtcct agagttgatg attccaattt tgacaatatc gaaaaaaaag aatttgatac
    34141 attcaaccgc gaagaaacaa aatctcatgg cgatgatgaa ttaaatacgc caccgttttt
    34201 ccgtcgtaaa aaataaagga gaggaagagc acatgattgc tgacaacttg cagaaaatca
    34261 agcaagaaat tcaggattcg tgtgctcttg ttcatcgtga tccgaaagaa gttacattag
    34321 tagctgtaac aaaatctgtt gaatccgctc aagctgaaga attggttaga gctggcgtta
    34381 tgaattgtgc agaaaaccga gtagataaat tattagaaaa gaaacaagat ttatttgatt
    34441 ttcctgaaat aaagtggcat ttaattggta atttacagcg cagaaaggta aaattgattg
    34501 taaatgaaat tgattacttt catgcgttgg acagtttaag attagcagaa gaaatccaga
    34561 agcggttaga gaaagaatta tcctgtttta ttgaagtaaa tgtttctggt gaggaatcaa
    34621 aacatggaat ttcaccagat gagcttttac cgtttgtgga atctttagct gcctgcacaa
    34681 aaatcaaggt tgttgggttg atgactatgg cgcctaaaga agcttctgat gagcaaatcc
    34741 ataaagtgtt tggtacgctg aaagaattgc gtgatcttgt tcaagcaaaa ggttatgccc
    34801 atgcaccatg tagtgaatta agtatgggga tgagtcaaga ctttcaaatt gcgattgaag
    34861 aaggtgcaac atttatccgt gtaggaacag cgctattcaa agacgaataa aggaggaaca
    34921 aaaatgccgc ttttaaacag aggagcaatt gctaattttt tcggattagc tgatgaggaa
    34981 gagtacgaat accaagatta tcctgaaaca acaaaaaagg ctgtacagcc aactaaaaaa
    35041 aatacaacac agaatcaatc aactcattct gtatctgctg cgaatactca tgaaaaacaa
    35101 aaaacggcga cttcacgtcc tgtaacgcat gaaagtattc agccggctga aaagaaaaaa
    35161 gtaattgaac tacatcaatc acctacaaaa actgtagcga aagaaagaac agcttcaaga
    35221 acctctacac aacctaaagc aaatcttgct ggaaaaatca ctgtaatgga accaagagct
    35281 tattctgaag ctatggcgat tgctaaacga atcattgctg gagaagcaac cttagtgaat
    35341 tttcatttag tagaagaaaa acaagcgaga agaatcgttg attttctaac aggaacagtt
    35401 tatgcactag atggagatat caaacgtgtt ggagatgaga tttttctttg tacaccgact
    35461 gggatggaga ttgacagttc aaccgctcaa tcttttgcag atgcgaattt gtttgatctg
    35521 taagaggagg aactacgatt tatactattg cactattact tcatttactt caacgggcag
    35581 cctatttcta tagcattatc ttgatggtat atgcattgct ctcttggttt ccaggcgggt
    35641 atcaatcagg gctaggacga ttattagcta agatctgcga accgtatatc agtttgttcg
    35701 atcgtctgcc tttgaggatt ggacccttgg atttcacaat tttaggggca atcctgatct
    35761 tgaacttagc cgtacaaggt ttagcacaga tcgttatatg gataactgtc catttagttt
    35821 attgattgaa gcaacaaaat ctttattctc agaatagata ggaggggaaa attttggatg
    35881 caaatgtata ccagcacttt cgggcagatg aacgtacatt tatcgattcg gtaggcgatt
    35941 ggattgaaca ggttcaagga caatatgcac cctatctgac cgaatttctt gacccaagac
    36001 aatcctatat tttagaaaca ctgattcgac aaagcagtga tttgcgtttc atgttttatg
    36061 gcggatatga acaggcagaa agaaaaagat gtttgatttt ccccgattat tacgaacctg
    36121 tagaggaaga ttttgaaaca gcattgtatg aaatccatta tcctagcaaa tttgccagcc
    36181 taagtcatgg gaaaatcctt ggaactttag tgggaacggg gatcaaacgc tcttactttg
    36241 gcgatatcat ctcggacggt gatcgctggc aagtatttat tgcaaatgag gtggatcatt
    36301 ttgttgctgc acaagtaaca aagattggaa aggttgctgt acgcttggaa ccaatcaaat
    36361 acacagaaat gattatgccg aaagatagct ggacacaaga aagaggaaca gttagttcca
    36421 tgcgactaga cagtgtgatc tctgagattt ataatatttc cagacagcgg tccaagcagc
    36481 tgatcgaagc tggtaaagta aaagtgaact ggacagaaaa tacaagacct gattttgtac
    36541 ttgaactatt agatattatt tcagtcagag gttatggcag agtccagatc caagaacttg
    36601 aggggaagac gaaaaaagaa aagtacaggg tactgttcgg tgtattacgt aaataaaata
    36661 aaaaggtggt ttactcatgg ctttaactcc attagatatc caaaataaga cattcccgac
    36721 aaaaatgcgg ggatataatc aagacgaagt agatgatttc ttagatttag ttgttcgtga
    36781 ctatgaggaa ttgactcaaa gaaatcgaga attagaaaaa gcagtcaaac attctgaaga
    36841 aaaacttgag tacttcaatg aattaaaaga tgctttgaac caatcaatca tcgtagctca
    36901 agatacggct gataaagtaa aaacaagtgc aagcaaggaa tccgaagtga tcgttacttc
    36961 tgcacaaaac aaagcagatg agttagttgc gaacgctgaa aaacgtgcac atcaattaac
    37021 aactgatgca gaagaaaaag caagaaaaat cttgacagat gcaacagaaa aagcaagaca
    37081 gcttgcgaca gaaactgaag atcttaagaa aaaaacacga gtattccatc aacgtatcag
    37141 cttgatgctt gagtctcaat tagaacaagt gaaaagtccg gaatgggatg aaattttaca
    37201 accattctcc agctacgtga ctgacagcca tgaagtgatc aaagaagttt tagcaaaaca
    37261 gcttgacaat gaaaatgata ctgatgtaaa ctcagacaca ggagtcatag aagcacctat
    37321 gacggcattt gatacacagg ctattgatat ctcgatcgtg cctggtgaaa atgaaaacga
    37381 gtagaacaga aaagcacttt gttattctta aagcgagccg atgagagtgg aagttcggta
    37441 agacactgca tggtgaaaga tcctctactt gagacctgct tttccgaaat tagagtaaga
    37501 agcagcggct gacctcgtta ttggtcacag agagtttgca ctagatagat gcaactaaat
    37561 ttgggtggta ccacggcatg ttcgtccctt gcggctgaac atgctttttt gtgtccggaa
    37621 aacaaataaa ggagtgtatg gttgaatgaa aatgaaagaa accttgcaat taggaaaaac
    37681 aaaattccct atgcgtggta atttgccaaa ccgagaaggc gagtggcaaa aagaatggga
    37741 agaaaatcga atctaccaaa aaagacaaga gttgaacgaa ggaaaaccta gctttgttct
    37801 tcatgatgga cctccatatg ccaatggtaa tatccattta ggacattcat tgaataaaat
    37861 cagtaaagac attatcattc gttcaaaatc aatgtctggc tatcgtgcac catatgttcc
    37921 aggatgggat acacatggat tgccgattga acaagtattg gctaataaag gcgtgaaacg
    37981 taaagaaatg tccatggcag aatacttgga aaaatgtcgt gaatatgcac tttcccaagt
    38041 agataagcaa cgagcagatt tcaaacgcct aggagttgca ggagattggg aacatcctta
    38101 tgttacatta gatccttctt atgaagctgc acaagtacgc gtatttggta aaatggctga
    38161 aaaaggctat atttacaaag gattgaaacc aatttactgg tctccgtcaa gtgagtcttc
    38221 tttagcggaa gctgaaattg aatataaaga tgtaaaatct ccatctattt ttgtagcttt
    38281 caatgtcaaa gacggcaaag atattcttga tgaagataca gcttttgtca tttggacgac
    38341 aacaccatgg acattgccag caaaccaagg tattgctgta aatccaaaat ttacgtatgt
    38401 attggtggaa gccgatggcc gtaagtttgt cgtagcgaaa gatttgctag aaacagtcca
    38461 atcagaaatc ggttgggaaa atgtaacagt tctaaaagaa tttgctggac aagaaatgga
    38521 atatatgact gcaagtcatc cattctatga acgtgaatca cttgtgattt taggcgatca
    38581 tgttacttta gacgctggta ctggattagt ccatactgct ccaggacatg gggaagatga
    38641 ctatatcgct ggtagcaaat ataaactatc tgtagtgtca cctgtagact caaaaggatt
    38701 gtttacagaa gaagcacctg gatttgaagg tatcttttat gacaaagcaa atcctatgat
    38761 cactgatcta ttaaaagaaa aaggagcttt gctgaagcta gatttcttta ctcatagtta
    38821 tcctcacgat tggcggacga aaaagcctgt gatctatcgg gcaactccac aatggtttgc
    38881 atctatcgat aaattccgtc agaatatttt ggatgaagta gaaaaagtag agtgggttat
    38941 cccatggggt aaaacacgtt tgtataatat gatccgtgac cgcggcgatt gggtaatttc
    39001 ccgtcagcgt gcatggggag taccacttcc gattttctat gctgaaaatg gagaagcaat
    39061 catcacacca gaaacaatcg aacatgtggc aaaattattt gctgaacatg gatcaattat
    39121 ctggtttgaa aaagaagcga aagaacttct tcctgaagga tttacccacc caggttctcc
    39181 taatggcgaa tttacaaaag aaaaagatat catggatgtc tggtttgatt ctggttcttc
    39241 tcatgaagct gttttacgtc agcgaccaga attatcattc ccagcagata tgtatttaga
    39301 aggatcagac caatatcgtg gctggttcaa ctcaagtatc acgactagtg tggctatcaa
    39361 tggtgtagca ccatacaaat cagttatttc tcaaggattt gctttagatg gtgaaggccg
    39421 taagatgagt aaatctttag gaaacatcat tgcaccagaa aaagtgatca aacaatttgg
    39481 tgcagatatc cttcgcttat gggtatctag tgtagattat gaagcagatg ttcgtgtttc
    39541 tatggatatc ttaagtcaag tggcagaagt atatcgtaaa atcagaaata cgatgcgttt
    39601 cttattagcg aatacagaag atttcgatcc taaaaaagat actgtttcgt acaatgattt
    39661 acgttctgta gacaaatata tgaccgttcg attgaaccaa gtaatcaaag aaatccgaga
    39721 agaagggtac gacaaatata acttcatgca tatttaccgt acagttatga acttcttgac
    39781 ggttgatcta tcgtcattct acctggattt tgctaaagat gtagtttata ttgaagcaga
    39841 agatgattat caacgtcgtt gcatgcaaac tgttttctat caaacagctg tagctttgac
    39901 aaaattattg actcctatca ttccgcatac agcagaagaa atctggtcat tcctgaaaga
    39961 agaagaggag tacgttcagt taagcgagtt tcctggttat gaagagtttg caaaccaaga
    40021 agaattgatg gatacctgga ctgctttcat ggacttccgt gataatgtct taaaagcatt
    40081 ggaagaagca agaaacagca aattgattgg taaatctttg gaagcaaaac taactgttta
    40141 tccaaaacaa caagttcgtg aaatgttaaa agctttggat gcagatattg cacaattatt
    40201 gatcgtttca ccagactact ttgaagttat ggcagcagat gaacaagtgc cagataacgc
    40261 tatggtattt gatgatgttg ctattacagt ggaaaaagca gacggggaaa cttgcgatcg
    40321 ctgtcgccaa gttcgtaaag atgtaggagt agatgaaaaa cttcctcatc tatgcggtcg
    40381 ttgtgcaaaa atcgttgaag acttctatcc tgaagctgta gctgaaggat tcgaagaaaa
    40441 gtaaaaggga aaagggggtg tgacatgttc tttgtcacac ccccttttgt tatcaatcgt
    40501 ttaattttcc acgttcacga taccagttat ctggttgcca gatccattcg aatccgtctt
    40561 tttcaagtag ttcgaaagct gctttaggtc ccattgatcc agctttgtag ttgggaaaat
    40621 ccggttgtgt ttggtcccaa gcttttcgaa tgacatccac gatatgccaa gattgagcga
    40681 cttcttccca atgagagaag ttcgttccat caccattcaa cgcatctaat agtaattttt
    40741 cgtaagcttc tgggctattt tcaaccattt ctgcactatt tctaaattcc aatttgattg
    40801 gttccgtttc aaatccttgc cctacttctt tcccattcaa ggataaagaa aatccttcag
    40861 ttggctgaat atagatcgtt agaacattag gtggaagtgt cgtatcgtca catggttcat
    40921 cgacagatgt tttgaataca ttaacaggaa cttgtttgaa tacgatgttg atccgagtcc
    40981 ctttttcagt caaacgtttg cctgtacgta catagaaagg aacacctgac caacggaagt
    41041 tatcgatagt gaatttgcct gcgacgaatg tttcagtctc tgattgagca tccactttgt
    41101 cttcttcgcg atagcccttg aattgtacac catccaattc accttgagca tattgcccgc
    41161 gaacgaagtt ttctaacact tcttcttcgt tataaatgcg gatagctttc aatgctttga
    41221 tcttttctgt acggatttct ttttcggaaa atgctactgg cggttccatt gcaagtaatg
    41281 ctacaacctg taaaatatga ttttgcacca tgtctttaag tgcgccacta tgatcgtagt
    41341 agcccccgcg atcttctaca ccgaggtttt cgccaaaagt aatttggata ttgtcgatat
    41401 agcgattgtt ccattgagcc tcaaaaatat tattggcaaa acggatagct gagatgtttt
    41461 ggatcatttc ttttcctaaa taatgatcaa tcctgaaaat atcttgttct gggaatactt
    41521 ctctgatttg ctggttcaat tcaaaagcag actggtaatc agaaccgaac ggtttttcta
    41581 tgatcaaacg attgaagcca ttttttgtca taatattttg tgatttcaaa tgagaaacga
    41641 tcgttccaaa aaattgaggt gccatagcca aataatagac acggttaccg tttaattgat
    41701 agtgtccatc taatcggtca gacaattctt ttaatgtctg ataatgcgtt gaatcattta
    41761 catcatggga ttgatagtaa aaatggcttg caaaggtagt tgcttcttca tctgaaggat
    41821 tcaatgcacg gattgtttct ttgacaactt cacggtagtg ctcatcgctc cagggacggc
    41881 gagcggttcc gattacagca aaatgctcgc ctaaatttcc ttttctgtaa aggcggaaca
    41941 aagacgggta caatttacgc tgggctaggt cacctgtacc gccgaatatc gtaaacaata
    42001 catttttttc attagacatg tggatacact cctttatatt ttatcgacaa aaatcgtgct
    42061 ggccgcctta aagctgacgg ggattttctt tctatcattt tggatagtga tcggtccttc
    42121 ataagctgcg atgtcaacga tctcatattc ttcgttgata ttcaggtcaa tagtgactaa
    42181 gtaatccaac aattcttttt catcaagcac tcgtgcgata cgcacttttg taccgacagg
    42241 ataggactcc aatgtttgtc ggcgttcttc atggacaact tcctcttttt taggaatcga
    42301 tcctccatga ggacaatgtt caggatgatt caaatagttt tctaaatgat ctgcaagatg
    42361 ttccgaagtc acatgttcca atacttcagc atcatcatgg acttcattcc agctatagtc
    42421 taaatgttca actaaaaaaa cttcccataa gcgatgtttg cgaatcagac tgctagcttt
    42481 ttgcagtccg cttgcagtca attgtacacc ttgataaggg gaatgttcta caagctcttc
    42541 tttcacaagt ttagaaatca tttcactgac agatgcagct gagacatcta gactagatac
    42601 gatttgttta ttgttgactt tcgttgtatc tccacctagt tctaaaatga ttttcaaata
    42661 atcttctcga tttggtgtca tattcgatca tcctttcatg gtaaaaaacc atcattagta
    42721 agtgtaacaa aaaaaacgtt aattgactat ttttctaaat aaaaaacaaa ggatttttag
    42781 acgtttttac aacaaaaaac gaaagatgat agagaagact cattctttcg tttataaagg
    42841 atatatatat acattgggag ggatctattt ttcagataac aacttatatg aagtgtctgc
    42901 agattcgtca taaagagttt gttcgggata agaaaagata agataaaaac catactgatc
    42961 acgtacgact aaacgatatt catctatttc taccacagat gtgccaagag actgaccgtt
    43021 gatcaaaaac gtgtaatcag atgtaatctg taattgttgt ttttgctggc tatcactaaa
    43081 gaaccatgta ccttgatact ttgagagtcc ttctgacttt tccgcttgtt ttttcttttt
    43141 caagtaaata aaacttgaga gaacggtaat gagtgaacca agaaaataga tcattttttt
    43201 tatcaccaaa tcactccttt atgagaaaat aatcgcatag acatattctt tcttctttat
    43261 ttcattgtac cgaataaaag ctaaaatttc tctaagactt tcgactggtt tttttactgt
    43321 ttttctacaa attatgatag gatacatagt aggaagaaag gtgacataat ttgaagtatc
    43381 agcaagtgga aatagctttt tttattgacc gcccgaatcg gtttatagca cattgtatga
    43441 aaaaagacgg agaaataatt attgcgcatg tcaaaaatac aggtagagga aaagaggtcc
    43501 tgataccagg cgctgaagtg gctattgtat ttgctcctgg acctaaacgg aaaaccgcct
    43561 atgatctgat tgctgtaaaa aagaagggtg actggtttaa tatcgatagt caattgccta
    43621 atcggctggc aattgatggt attttagacg gcacgatcct tttaccagga ttaactggtc
    43681 catttacggt atacaagcgt gaagtaactt atcttcactc caaatttgat atttatttag
    43741 agacggtggt cggacaaaaa gcatttgttg aagtcaaagg tatgacactt gaaaataaag
    43801 cgattggtgc ttttcctgat gcgcccacac tcagaggatt gaaacatata ggagaattaa
    43861 cgtatgccgc acaagatggt tatgccgctt atgtcttgtt tattgctcag ttcgaacatc
    43921 ttcatctggc aacgatccat gaagaaatgc aaccagcact agctgatatg gttcgacatg
    43981 cgcagcaaag cggagttcaa atcctcgcct ataattgtca agtatcggca gatgaggtaa
    44041 ctgtcaagca ggccattcca tttgatgtag atatcccttt tgaagatcct atacaataga
    44101 aagaaggtaa aataatgatc aacttaggta ttatcggaac aaattggatt actcatcaat
    44161 ttgtccaagc agctttaagc acaaaaaaat atgaattgac agcggtatat tctagacatt
    44221 tggaaaaagc acaggaattt ggttctcatt atgaagggga tatcgaattc gcaacagatt
    44281 tgaagacttt tttcaatatt actcatatgg acaccattta tattgcttct ccgaatagtc
    44341 ttcactttag tcaagctaag caagcaattt tggcaggaaa aaatgtgata gttgaaaagc
    44401 cagctttttc gacgccaaaa gaaatggatg aaatcatcca tttggcaaat gaacacaatg
    44461 tgtatttttt tgaagcagca agaaatatcc atgaatcagg ttttaaaaag attgccgatt
    44521 acttaccatt aaaagacgag ataatcggtg caaatttcag ctatatgaaa tattcttcgc
    44581 gctatgacca agtattagat ggagaagaac cgaatatctt ctcgactcat ttctcaggtg
    44641 gtgcattgat ggatttaggt gtttaccttg tatatgcagc tgttgcttgg tttggaatgc
    44701 caaaagaagt ccattatttc ccaagaaaga tttctactgg tgtagatggt cttggatggg
    44761 ggatcttacg ttatgagaat tttgatgtat cgatccagcc aggtaaaatc ggtgattctt
    44821 atttaccgtc agagatttat tttgactcag gaacgctagt gatgaatgga gtcaatgcaa
    44881 ttgaacaagc acaatttcat gatcgcgcgc ataaagaaat cattgatttg gcggtaacag
    44941 ctgcagaaaa tccaatggca gatgaagcag aggattttgc caatgtcatg gctcatccga
    45001 aagatccagc ttggggattg ttgtatgaag agtggatcga gctagctaga aatgtgaacc
    45061 aagtgattta tgatttaaga aaaaatggcg gcatcaaatt cgatgcagat aatgaggaag
    45121 atttttaatg aacgaatttg tacaagtatt tcccgaaaat tggcagaaaa aatatgagga
    45181 taaagggttt atctcacctt ctcttattca acgagcagtt tttcagccac taagtaataa
    45241 acaaagtata gtagcggttt ctcctacagg ttcaggtaaa acactggctt atctttggcc
    45301 tctactacta aacgtggaac caggtgaagc aagtgcgttg gtcatttttg cttcttctca
    45361 agaactagct attcaagtag cagatgttgc acgtgaatgg ggaaaagaca aagagttgaa
    45421 agtccaatct ttggtgggcg gagccaacgt caaacgtcag attgagggtt tgaaggaaaa
    45481 accagaaatc ttgattggta caccaggaag aatattggaa ttgatgaaag caaaaaaaat
    45541 aaaagcacat caagtgaaaa caatggtatt tgatgaagca gaccagctat ttgacgctgg
    45601 gaacagccaa atcattgatc aaattcttca tcaagctcca acagagtatc agctagcttt
    45661 tttttcagcg acagcagatc gttcccttga aagcattgaa aaaatcactg ggaaaacgtt
    45721 tgagacaatt gacgtgacag cagaggacga ttctcgaaaa ggactgagac attacttttt
    45781 acgtgtaccg atacgaaaga aagatgacta cttgagacgt ctcacgcata tagaagattt
    45841 tcatggtctg attttcttta atcaactgac tgaattaggc gtaatggaag aaaaattgct
    45901 gtatagaggt gtaccggtag gaagtctggc ctctgatcag aacaagctac tccgaaaagc
    45961 agcgctagaa caattcaaaa agaaaatggt cagagcactt ttgaccacag atgtggcggc
    46021 tagaggttta gatattacag gtcttcctta tgtagtgaat gcagaagttc cgttaagtga
    46081 agaagcttat ttgcatcgtt cgggaagaac tggtcgaatg ggtaatgaag gaatagtaat
    46141 tacactcgta caagataata atctaagaga tttaaaacgg cttttacgtc catcaaatat
    46201 aacacttgaa gagatttttt tatacggagg taagctgcaa actgagcagc caaccaaaga
    46261 agatgcagct cctgcagtaa ataaaaagta tgcgaaaact ttttctggaa aggagcagaa
    46321 cccagaatat aacaaagaaa aaataagaaa aaaagttaaa aataaatcaa aaaaagagag
    46381 aaataaagga gcaaggcgca aataagcgcc tttttatgct atgattatga aaacgggaac
    46441 aaaattggtt gccagaggag gggttatgtg aaagaacgtc aagaagagat cttgcagcta
    46501 ttgacagaaa accaacaagg attgacagca agtgatgtag ctgagcgctt aactatcgat
    46561 cgaagcaatg caagccgata tttaagtgaa ctatacaagg cccatcatat tgtaaaaacc
    46621 gctggccgac cagtcgttta ctctttgcca acggaaaagt caaaatctga tgaagtgcat
    46681 gtcgactctt ctacgcaagt gacttttgaa acactcgttg gagaaaatga ttctttaaaa
    46741 gtcagcatac agcaggcaaa agctgcgatt ctttatccac cgcgggggtt gcatacgatt
    46801 atttttggag aaactggtac agggaagtcg atgtttgctg aatgtatgta tcattttgca
    46861 atcgattcag agatgctttc tgcagatgcg ccatttgttt ctttcaattg tgcggattat
    46921 gctcaaaatc cgcaactgct ctttggacac atctttggga tcaaaaaagg ggcttacaca
    46981 ggagcagctc aagatagtcc cggtctaata gccaaagcag acggcggtat attgttttta
    47041 gatgagatcc accgtttacc gccggaaggg caggaaatgc tgtttacttt cattgataaa
    47101 ggtatttacc gtccgcttgg agaaagtgca caagtacatg aagcatctgt tcagatcatt
    47161 ggggcgacga ctgaatcttc agaaagtttc ttatctacgt tcaatcgacg gattcctatg
    47221 gcaatcacct tgccgaatct tgcttcacgt tcactggatg agcgttatga aatcatctct
    47281 ctttttatca aacaagaagc gaatcgtttg aatcaacgta tcgatatcga aaaagaagcg
    47341 attcttgcct tcatgcttta tgatgcggaa ggaaatatcg gtcaagtgaa acgagacttg
    47401 aaactagtct gtgcaaaatc ttttttacat taccgtactc atcatgagaa aaagctaatc
    47461 attcgaaaag aagaactttc ccttcaagta caaaaaggat tactgaaaat caaggaaata
    47521 ccggaacgtc tcgaccgatt catggacagc aaaagtcagt atttgacatt tgaaccagga
    47581 tttgctgatg ttgtctggtc acaagatcca gaacgaaaca tgcaggtcta taacgatatt
    47641 gaggaaaaag tcacttctct ttccgaaacc ggtgtggaga acattgatct agagaccctc
    47701 atttcaaaag atgtggatgc ttatttccag acatatgtca aagaattgac taaaagttcg
    47761 attcccaaag atcttttgcc agatgaaatc tggcggttaa ccaatcaatt gtatgatgtg
    47821 gctgaaaaac ggttagaccg tatttataat gaaaaagctc gttttgcttt tgctttgcat
    47881 ctgcaaagta cattggatcg tgtgagagaa ggtcatatga tcgtccaccc agatttgaat
    47941 aacgtacgga aaaattttaa gagggaattc caggtagcaa tcgatctttc aacgatcatt
    48001 gaagaagaag aacaagttga gattcctttt gatgaaatcg gctttattag tatgtttctt
    48061 tctatcaatc taggagaaag tgagcctgtt aaagaaaaca aagtagatgt tctcatattg
    48121 atgcacggca gacagactgc ttcaagcatg ttggagatgg cacaagaact attaggaaca
    48181 aaagtaggga aagcttttaa tatgccactg gttatggaag ttcaaacaat gtatgaagaa
    48241 gtgttacaat atgtcaaaaa ctatcaagag catttgacga atggcctact cttgttaaca
    48301 gatatgggat cattaaacac cttcagtgat ttgatttatg aagagactgg cattcggacg
    48361 aaagctatca cgatgacaag taccatgatt gtgatagaag cgatacggat ggcaagtgtt
    48421 ggaagaagct tagaagatat ttatcaaaat atccagcttt cctttgaatc gatcgtacgt
    48481 gatcagttca aagcggaaaa gtctgaaact ttacagcgta aaaaagccgt aatcgtgaca
    48541 tgctttactg gtgaaggagt agctgccaaa ttatatcagc gtattatgcc agtgattaat
    48601 caaaataagg tagaactgat ccagatgcag tttttagaac gtgaatcatt caaaaaacac
    48661 atcgatgcct tattggaaga atatgagatc aaagcaattg cgggaacagt tgatgttgat
    48721 tatcaaaata tcccattttt ttcagcctat gacgtttttg atgatgaaaa actaaatatt
    48781 ttaaagcgta tagctgatga tgagatcccg acagctaaaa ttgtcgattc acttgctgga
    48841 tcattaagcg atgtcttttc tattgatgaa ctgatccgtt cattgcaaaa gacagttcag
    48901 cagatccaga ccgatttgtc gattatcgtg gaacctggag tagatactgg aatcgttatc
    48961 catttagcct tcatgttaga cggattgatc aaacaagaaa gccatcggca atttgagaaa
    49021 ctgtctattt tctcaaaaat ttatcgtttg gaaatggata tcgttcgggc aaacttgatg
    49081 gcactagaac gaaaatatca ggtaaaaatt ccagaagatg atatcgcatt tcttacacaa
    49141 atgtttcttg aaaataaaat aaaaaaatga acacactaat catactgttt acacactaaa
    49201 catctgtgtg cgcactataa aaaagagcta aaaagcatga aaacgctatt tcagctcttt
    49261 tttttattgg catgcttctt gcgttatata aaagtgtaaa aaaataaaag gagtgttaaa
    49321 aatggcaaaa aaaacaatca tgttagtttg ttcagcaggg atgagtacga gtttattggt
    49381 cacaaaaatg caaaaagcag cagaagaaaa agggatggaa gccgatattt ttgcggtctc
    49441 tgcctcagat gcagataata atttagaatc taagaatgtc gatgtattat tattaggtcc
    49501 tcaagtacgt ttcatgaaag ctcaatttga acaaaaactt gcgcctaaag gtattccatt
    49561 agatgtcatc aatatgcaag actatggcat gatgaacgga gaaaaagttc ttgcacaagc
    49621 agaaaatcta atgaaataaa tcaagtatgc agttgggggt ataacaacgt ggaagatcaa
    49681 caaaatctag aagccatcat ggggttaatc atgtttggcg gtaatgcaaa gagcgatgca
    49741 atggaagcga ttgcggctgc taaaatcggt gattttgagt tagcggatag gaaaatcgct
    49801 gatgcagaag aatcacttat acaagcgcat catgcacaaa cagacatgtt gacacaagaa
    49861 gcacaaggaa atcatatgca agtgacatta ttgactgttc acagtcaaga tcacttaatg
    49921 acatctattg cgtttactga tttagcaaaa gaaatcatcg aactttatcg tcgcgtatac
    49981 agcgaataaa tacaacttct tttttgaacg gaagaagaaa aatttttgaa cggatttttt
    50041 attttgttat aaggagaact ctctttatat aaaaaactta taggaggaag taaaaaatga
    50101 acggactatc aagttggatg gagaaataca tcctgccggt tgccgcaaaa atcggagcgc
    50161 aaaaacattt agttgctttg cgtgatgctt ttatcggcat actgcccgca acaatggcgg
    50221 gatcagtcgc tgtaatgatc aatgcaatta ttcgtgattt accgcaacag tttaacagta
    50281 cctatgctga cagtttagca gcagacaaag gaatctttgc cgtattgaat gtcatcatcg
    50341 gaatcaatgg ttttgtctgg aacggtacac tagcaatcgc cggattgatc tttgcctttt
    50401 cttgggggta taatcttgcc cgagcttatg gagtaaatga tctagctggg gggatcgttg
    50461 gtttagctac cttgattcaa ggtatttcct ttggtccagc attatcagca gcattagatg
    50521 tgaatgttcc ttcaaatgtc gcagatgcaa tcaatagttc tgagattggt gcaacgattg
    50581 cagatggcac actgtcagtt ggattatggg gttggctaaa actagatcat ttgaatggaa
    50641 acgcttattt tacagttatg atcatgggag ctatttcagt cattattttt tgtaaattga
    50701 tgcttgccaa cattacgatc aaaatgcctg attcagtacc accagcagta tctaaagcat
    50761 ttgcagccat tttaccagcg acgatcgctt tatacgtcat tgctattatc aattatatcg
    50821 taggtcgtgt agctgatggg caactgatca ttgatttggt gcaaaaatat attgcagaac
    50881 cattcctagg attatctcaa gggttgggag cagtattgat cgtcacagta tttgttcaag
    50941 tattctggtt ctttggtatt catggatcaa atgtattggc accagtgcta gagggaatct
    51001 ggggacaagc acaactaatc aatattgata ttttccaaaa aggttataac ggaaaaactg
    51061 gtacaccagc cgtgttagat gcaatcgaag atggaaaagc ttatatgtgg gtccgcggat
    51121 cgttcgatgc ttttgcatgg tttggcggat caggcggaac gatcgtctta ctgatagcta
    51181 tcttgatttt ctctaaacgt gcagattatc taacagtagc taaattatcc attgggcctg
    51241 gtatcttcaa tatcaacgaa ccagtgatgt ttggtctgcc aatcgtattg aatgcaatca
    51301 tgtttatccc attcttgatt gctccagtag tagcaacagc aattggatgg acagctactt
    51361 atcttggcct agtcgctccg gtatcgcagc aagtcacatg ggtagtaccg ccgttcttat
    51421 tgtcattctt agcaacagga gcggattggc gggcaccgat cgtaacatta gtatgtatgg
    51481 tcgtgacttt cttgatttgg gcaccatttg tcatctctgc aaacaaaatg gaccctgccg
    51541 caggcgaaca acaataagat gaacgtgagg agcgggacgc tttaagtttc gttcctcata
    51601 atgaattttc gaggaggaac tgaaatgatt acaattgcca gtgcaatgca acaagatgaa
    51661 atcaaagaat tgttagaagg atacaatgaa gaaggaatga cttttacatt taaagaaaaa
    51721 caaggaatca agctgttctt tgaaacaagt gcagaagatc cggaagttgc ggctaaaaaa
    51781 gcaaaggaac taatcaaagc acagccttgg ggaacagtcc tttattttca atcaacagct
    51841 gtttagaaaa tggtttaaaa agataggatg gttgagatga taaaaaacgg tttattttta
    51901 tgtagtattg gttctttgat cgtgttgtat agtttgaatc cacattcgca aaggtatgat
    51961 ttgtatagta tggtcacagg tctttttcta acaggattag gaatctattt cttcatgaag
    52021 gggaaaaaaa gagaagaagc aaaggagaaa aataactaat ggaaatcacg aaaaaattaa
    52081 atattccagc atcgtttttt tacgataaag ttatggactc tgtccttttc gatgtacgta
    52141 aagcgaccgg caaatcagta actagaaaac agctgaaaaa tttggagtac gtcaaacaat
    52201 tctcgcaaaa taaccgggct cgcataaaaa tcgaagaagt cattgaaaac cagtcttata
    52261 agtttcgtac atcgacaacg aagaatgaat tcgtcgttca ttatcaaatc aaaccattgg
    52321 acgattcgac ctgtgaggtt cattacacag aaaaaatgga gtcttatggc atgcttcaaa
    52381 agttgaatga tatgctttta ggaacaatct tgatgtattt taaacggaga cgtttcaaaa
    52441 aaatgctaga tatgatcgaa gcaacgtatt aaatcaatga aacatggtac tatttagtta
    52501 ttgaaaaatg aagtaagcta gaaggaattt ttcataggaa gatagatctt tcctgcgaat
    52561 cctcactagc ttttttgtag gaaggggcag caaaatgaca aacaaagtaa gagagctagg
    52621 tgtatcttcg attgaggaaa tgtttgattt agccacttat gcatttaatg gggcagatac
    52681 acctgaaagg cgagaacgtt ttcgtacact tgcggagaat tcttggaatt acggattttt
    52741 tgatgaggag gatcggctaa caagtcaagt catggcgacc ccttttccag tgaattttta
    52801 cggacaagag tacctaatgg ccggcattgg ctatgttgct tcttatccag aagcaagggg
    52861 acaaggcggg atcaatcaga tcatggaaaa gatccttgaa gattgccgtg atcgtaaagt
    52921 aagcttatct tatctggcgc cattctccta tcccttctat cggcgctatg gctatgaaca
    52981 atcatttgat aaaatcagtt atcaactaaa cagtcgtgat gtgccgttta tcaaaaaaaa
    53041 atctggaaaa ataaagcgta tggattttga agatgcaaaa gctgcaatca gaagaattta
    53101 tgaacagatg ccggaaaacc aacgagctgg tctgaagcgc gaagactggt ggtatacgta
    53161 taaattcaag caaaaaggca attaccagtt tgctttgcat tttgatgata atgaggaagc
    53221 aacgggatat atcgtttatc agcttgcaac gtcttccttt attattgcgg agtggggatt
    53281 tttggatcat gatgcttttt gcagccttgt tcgcttcgta agttctcaca atggtgcttt
    53341 tgaaacattt gaatattcat gcggctacgg cggtaatgat caaaattatc tattagaaaa
    53401 tccatttgct tctgtaatga tcacacctta tatgatggcg aggatcgtag atatttcttt
    53461 gtttttggaa aattatccat ttaaaaagag acaagctgca tttgccctac aaatagaaga
    53521 agatcattat gcgaaatgga atgaaggaat cgtggaagta acaatcgaaa atggaaaaaa
    53581 tcatctctca aaagtagaac aaacaaaact cccaatcgtt cgaggaagta tccaacatat
    53641 cacgcagcta ttattagggt atcgtaaagt ggaagagttg gtattccaag aaagaataca
    53701 ggtgacaaaa gaatatgaag aaacgttaag ctcaatcttg ccacagcaac agccaattct
    53761 taatgactat ttttgataaa aaacgggtga tgacaatatt tttgtcacca cccttttgtt
    53821 tttcatttgg catttgaagg aaaatgttaa agtaaatcct cttttttcgt aaatttgaat
    53881 tttttgtttt cttttgtttt agggttttct tttttgtggt cttggattgc ttctttcgta
    53941 atcttttccc gatttttttt agatgaatct aagcgttctg cgtatacttt ttcttttggc
    54001 ttatcattca ttggaaagga cctccctaca ttatttcatt ctacctctag tttatcatct
    54061 gttaaggact agtggaaacg ttttgcctaa cttataagct gattttttta gcagataagc
    54121 ttgcaagtaa aaaacaaaca agctacaatt gatttattga aattgttcct gtagctcagt
    54181 aggatagagc aatcgcctcc taagcgatag gtcgctggtt caattccagt caggaacgta
    54241 taaataaccc ctgtatcctt tgcggtacag gggtttgttt tgtatttgtg ccaaaaatgt
    54301 gccaaactag ttatcatttt tatataaact atcgaacaca gtattgattt tttctttatc
    54361 ctgatcttca agctctcgaa caatatgaag ataagtagac attgtagttt ctaaactgct
    54421 atgtcctaaa cgtttcgata cgctaagtat atttactcct tgataaagta aaatagatgc
    54481 atgcgtatgt cttagtccat caataaacag tcgaagagaa aatagggaaa aattgattac
    54541 taaagaaaaa cttactaggt cgacggtgta gttgattttc atacttcaat agattcgaat
    54601 taaacgattt cgattggctt ataccagtcc tatccaacga aagatatatc gttcttaact
    54661 ctagcggtat aacttgaagc tttacgtaaa gccaatggga atgaactgga gatgactttt
    54721 tattattttt agtaatagct gatgggttaa tacttacaac tttccttttc aaagtaccat
    54781 ataaaaaaac ggtggggttg tttagcgaaa taaaggatct aagccaatag cttagatcct
    54841 ttattctatg ctttata
//
LOCUS       DAYYGS010000007        52348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000007 DAYYGS010000000
VERSION     DAYYGS010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.007"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..1581)
                     /locus_tag="ACHA44_000407"
     CDS             complement(7..1581)
                     /locus_tag="ACHA44_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFM8903728.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="ACHA44_000408"
     CDS             complement(1836..2345)
                     /gene="mreD"
                     /locus_tag="ACHA44_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFM8903729.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="ACHA44_000409"
     CDS             complement(2349..3203)
                     /gene="mreC"
                     /locus_tag="ACHA44_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFM8903730.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(3358..3996)
                     /locus_tag="ACHA44_000410"
     CDS             complement(3358..3996)
                     /locus_tag="ACHA44_000410"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HFM8903731.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3997..4647)
                     /gene="rpe"
                     /locus_tag="ACHA44_000411"
     CDS             complement(3997..4647)
                     /gene="rpe"
                     /locus_tag="ACHA44_000411"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFM8903732.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="ACHA44_000412"
     CDS             complement(4660..5559)
                     /gene="rsgA"
                     /locus_tag="ACHA44_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFM8903733.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="ACHA44_000413"
     CDS             complement(5724..7793)
                     /gene="pknB"
                     /locus_tag="ACHA44_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HFM8903734.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7790..8530)
                     /locus_tag="ACHA44_000414"
     CDS             complement(7790..8530)
                     /locus_tag="ACHA44_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HFM8903735.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="ACHA44_000415"
     CDS             complement(8543..9901)
                     /gene="rsmB"
                     /locus_tag="ACHA44_000415"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFM8903736.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9901..10839)
                     /gene="fmt"
                     /locus_tag="ACHA44_000416"
     CDS             complement(9901..10839)
                     /gene="fmt"
                     /locus_tag="ACHA44_000416"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFM8903737.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10832..11323)
                     /gene="def"
                     /locus_tag="ACHA44_000417"
     CDS             complement(10832..11323)
                     /gene="def"
                     /locus_tag="ACHA44_000417"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8903738.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(11338..13746)
                     /gene="priA"
                     /locus_tag="ACHA44_000418"
     CDS             complement(11338..13746)
                     /gene="priA"
                     /locus_tag="ACHA44_000418"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFM8903739.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13999..14304)
                     /gene="rpoZ"
                     /locus_tag="ACHA44_000419"
     CDS             complement(13999..14304)
                     /gene="rpoZ"
                     /locus_tag="ACHA44_000419"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8903740.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="ACHA44_000420"
     CDS             complement(14309..14923)
                     /gene="gmk"
                     /locus_tag="ACHA44_000420"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8903741.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(15410..15694)
                     /locus_tag="ACHA44_000421"
     CDS             complement(15410..15694)
                     /locus_tag="ACHA44_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903742.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15712..16602)
                     /locus_tag="ACHA44_000422"
     CDS             complement(15712..16602)
                     /locus_tag="ACHA44_000422"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFM8903743.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(16622..16840)
                     /locus_tag="ACHA44_000423"
     CDS             complement(16622..16840)
                     /locus_tag="ACHA44_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HFM8903744.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16810..18510)
                     /locus_tag="ACHA44_000424"
     CDS             complement(16810..18510)
                     /locus_tag="ACHA44_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFM8903745.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGRGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18777..19550
                     /locus_tag="ACHA44_000425"
     CDS             18777..19550
                     /locus_tag="ACHA44_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903746.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="ACHA44_000426"
     CDS             complement(19631..20200)
                     /gene="thiT"
                     /locus_tag="ACHA44_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HFM8903747.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(20266..20368)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(20374..21345)
                     /locus_tag="ACHA44_000427"
     CDS             complement(20374..21345)
                     /locus_tag="ACHA44_000427"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8903748.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="ACHA44_000428"
     CDS             complement(21439..22812)
                     /gene="glmU"
                     /locus_tag="ACHA44_000428"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFM8903749.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(23315..24133)
                     /gene="purR"
                     /locus_tag="ACHA44_000429"
     CDS             complement(23315..24133)
                     /gene="purR"
                     /locus_tag="ACHA44_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HFM8903750.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(24300..25124)
                     /locus_tag="ACHA44_000430"
     CDS             complement(24300..25124)
                     /locus_tag="ACHA44_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8903751.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(25097..25777)
                     /locus_tag="ACHA44_000431"
     CDS             complement(25097..25777)
                     /locus_tag="ACHA44_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8903752.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25800..26738)
                     /locus_tag="ACHA44_000432"
     CDS             complement(25800..26738)
                     /locus_tag="ACHA44_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFM8903753.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26983..27834)
                     /gene="ispE"
                     /locus_tag="ACHA44_000433"
     CDS             complement(26983..27834)
                     /gene="ispE"
                     /locus_tag="ACHA44_000433"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFM8903754.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLGDHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="ACHA44_000434"
     CDS             complement(27988..30561)
                     /gene="mprF"
                     /locus_tag="ACHA44_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HFM8903755.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30702..31508
                     /gene="proC"
                     /locus_tag="ACHA44_000435"
     CDS             30702..31508
                     /gene="proC"
                     /locus_tag="ACHA44_000435"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFM8903756.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(31587..33143)
                     /gene="rny"
                     /locus_tag="ACHA44_000436"
     CDS             complement(31587..33143)
                     /gene="rny"
                     /locus_tag="ACHA44_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HFM8903757.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(33554..34603)
                     /gene="recA"
                     /locus_tag="ACHA44_000437"
     CDS             complement(33554..34603)
                     /gene="recA"
                     /locus_tag="ACHA44_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFM8903758.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34712..35953)
                     /locus_tag="ACHA44_000438"
     CDS             complement(34712..35953)
                     /locus_tag="ACHA44_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFM8903759.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="ACHA44_000439"
     CDS             complement(36193..36771)
                     /gene="pgsA"
                     /locus_tag="ACHA44_000439"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFM8903760.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36980..37903)
                     /locus_tag="ACHA44_000440"
     CDS             complement(36980..37903)
                     /locus_tag="ACHA44_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HFM8903761.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(38642..39928)
                     /locus_tag="ACHA44_000441"
     CDS             complement(38642..39928)
                     /locus_tag="ACHA44_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8903762.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39921..41189)
                     /locus_tag="ACHA44_000442"
     CDS             complement(39921..41189)
                     /locus_tag="ACHA44_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFM8903763.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            41441..41902
                     /gene="mscL"
                     /locus_tag="ACHA44_000443"
     CDS             41441..41902
                     /gene="mscL"
                     /locus_tag="ACHA44_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFM8903764.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41961..42407)
                     /locus_tag="ACHA44_000444"
     CDS             complement(41961..42407)
                     /locus_tag="ACHA44_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8903765.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(42500..42745)
                     /locus_tag="ACHA44_000445"
     CDS             complement(42500..42745)
                     /locus_tag="ACHA44_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HFM8903766.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42860..43438)
                     /locus_tag="ACHA44_000446"
     CDS             complement(42860..43438)
                     /locus_tag="ACHA44_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8903767.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(43515..44045)
                     /locus_tag="ACHA44_000447"
     CDS             complement(43515..44045)
                     /locus_tag="ACHA44_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFM8903768.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="ACHA44_000448"
     CDS             complement(44049..44909)
                     /gene="rlmB"
                     /locus_tag="ACHA44_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFM8903769.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44902..45297)
                     /locus_tag="ACHA44_000449"
     CDS             complement(44902..45297)
                     /locus_tag="ACHA44_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HFM8903770.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(45294..46706)
                     /gene="cysS"
                     /locus_tag="ACHA44_000450"
     CDS             complement(45294..46706)
                     /gene="cysS"
                     /locus_tag="ACHA44_000450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFM8903771.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46703..47239)
                     /gene="epsC"
                     /locus_tag="ACHA44_000451"
     CDS             complement(46703..47239)
                     /gene="epsC"
                     /locus_tag="ACHA44_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HFM8903772.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(47325..47564)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(47561..49021)
                     /gene="gltX"
                     /locus_tag="ACHA44_000452"
     CDS             complement(47561..49021)
                     /gene="gltX"
                     /locus_tag="ACHA44_000452"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFM8903773.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(49191..50348)
                     /locus_tag="ACHA44_000453"
     CDS             complement(49191..50348)
                     /locus_tag="ACHA44_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HFM8903774.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(50382..51752)
                     /gene="radA"
                     /locus_tag="ACHA44_000454"
     CDS             complement(50382..51752)
                     /gene="radA"
                     /locus_tag="ACHA44_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFM8903775.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51815..52342)
                     /locus_tag="ACHA44_000455"
     CDS             complement(51815..52342)
                     /locus_tag="ACHA44_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFM8903776.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT        14205 a        11400 c         9368 g        17375 t
ORIGIN      
        1 tcattcttac atgctgacag caaagtcagg ggcaaaccat tgaactgaac caacttttac
       61 gctttcgcct ggttgaggag catggatata ttgtcctccg cctaaagcaa tcgctacgtg
      121 gtaagttccg ccttgtgacc cccagaataa caagtctcca gcttttgctt gagaaactga
      181 aatttttgtt ccagctgatt cttgaggaac tgtccaacca ccgatgtcac gaccagttac
      241 ttgcatgtaa acatagcgtg tgaaccctga gcagtcaaag ccacttggat ctttaccgcc
      301 ccaaacatat ggagtaccaa tatatttgta agcttctgct acgattgctg caccattcac
      361 acttccagaa ggagctggag caggtgttac aggttttggt tgttcaggtg ttggtgttgg
      421 cgttggagcc ggagtactac ttcctgtgcc gtttcctgta tcaacacttt gatctgttga
      481 tggtgttgta ggcgcaggtg ttgttgattc ttctgttgtt gaagattcag gcactgttgt
      541 tgattcttct gttgctgaag attcaggcac tgttgttgat tcttctgttg ctgaagattc
      601 aggcgctgtt gttgattctt ctgttgctga agattcaggc gctgttgatt cttctgttga
      661 agattcaggt gttgttgatt cttctgttgc tgaagattgt gtagctgatg atgattcttc
      721 agtcattgaa gattgtgtag ctgatgattc ttctgttgct gagctttgtg cagtagatga
      781 agcttgagta gcttgtgctt cagcctctgc ttgttcgcgt gcttcttttt cagctttttc
      841 ttgtgcagct tgttgacgag cttgttctgc tagacgttgt tgttcacgaa tacgagcttg
      901 ctctgcttca gcttctgctt tttgacggtt caagtcagct ttcttgtctt cagcagttgc
      961 ttgttcagca gctaatgaag ttttcagtac gttcaaatca gcttgtttag ataaaagatc
     1021 gcctttttga gattccaaag cagcttggtt ttcagctaat tcttttagtt tagcatcgtt
     1081 ttcagctttt ttgtcttcta cagctttttt atcttgtttt tgttgttcca tcaagtcgtt
     1141 gtttgctttt accatcgttg tcattgcttg aacgcgtcca atagcatctg ctaatgaatc
     1201 agcattcaat acagcatcga tgtagtttga gcttgtgttg cttacttgtg cttcgcgtgc
     1261 ttgtttttgg attgtatctt cacgtttttc aatacgttct tgtaaatctg caatgtcttt
     1321 tactaattgt gcagattctt gacgcaatgt atcttgtttt gctaataggt cttgtgcttg
     1381 tgtgttgatt tcagaaactt ggctttctaa ggcatcgatt tgtgattgtg catctgcttg
     1441 ttgatttttc aagtctgcaa tcttttgatc ttgctgttgt atctgagaat caaaatcgtc
     1501 cgccgcggca gcgatcggtg atgctacggc agtcaaggtc attgaacata ccattactgc
     1561 tgatattaaa ctctttttca ctcttcattc ctccgactgg cttaatttaa taaatatcta
     1621 aatatattta agtctttctt aacgatataa aaattgtacc atagcagtgt gtcaacgata
     1681 taatagttat gttacaaaaa tgtttcaaaa caacttttaa aaatgacaaa actgttcgtt
     1741 tcagaaaatg atcatgaaaa agccgcgtta agtctagcat ttcttaagat aacaacgatt
     1801 ctttttcgct ttcgacctaa aaataaaaaa agaaattatt catcactgaa taatttctta
     1861 aagggaaaaa tgaataatac aaatataatc atgttcaaca gcagtgtagg tcctaaaaac
     1921 cttgtaataa aatacgtgtt atttactact gctaatttaa aaatcaactg gaccaccatc
     1981 gtaaacaatt cataacccgt gacaaagatg atcatactga aaaattcagt aaagatgttg
     2041 acatggataa cgtctttcat tacatacatc aaccatacaa ttaaagggag agctactgca
     2101 tagataccga taacgccaat atagtaagcg tcatagatag ccccgagaac aatcgtggta
     2161 atcagtaaat aacgtttttc aaacgccata ctgcaacata ataatgccaa tatcagaaag
     2221 tgggcatttg acatataggt gcctttcgac cactccccta gcattcttgt taaatgacca
     2281 tctatcaaca tcaaaaagaa caaaacgatt ggcagatagt acttcattgt ttctttcttc
     2341 aacatggact aatcctccgc tagccgttgg acaatcgtca caactgacac gtcatacatt
     2401 tctgcataag gtttcacata aacttcccga tccagcccat aactatctgg tttcgtttta
     2461 ataaccgtac cgatcggcaa gttagctgga gaattccctc caagaccgga tgtctggaca
     2521 acatcccctt ctttgatatc cgtatcacca gttaattggg tcaccactaa ggcatggagc
     2581 ttttcatcat agtttttaag caaaccaaac gcttcgccat tagccgaaga tacccgtact
     2641 ggaaaatgat tggagctttc attagaggat gacagtaatt cgattttaga cgaagccgta
     2701 ttgacctcga tcactcggcc aatcaatcct ttttgcgaca tgactgccat atttgcttcg
     2761 ataccgtctt tagaaccctt gtctacgatc agcatgtcct gccaactgtc aggcgaacga
     2821 gtaatgacat ttgccgttac tttttcgtaa ctagtcagcg tctgattcaa ctcaagttct
     2881 tttttcaacg cttcgatttc tctctcgtaa ttcttgtttt gctgtgaaac gtcatcatag
     2941 ctatcgattt tttcttttag ccgctcattt tcggcataag ttgaaaaaag attatggaca
     3001 gaatctactc catttcgtac ccaccgtact ggggcagaga ttgttttatc aaccattgca
     3061 acactgtcat taacgatgga ttggaagaaa ttgggctttc cttcattcgc tcgctgcgca
     3121 gcggtcacac ttagtatcgt caccacgatg ataacaataa ccaaggtaat aataatattt
     3181 ttattaggat tgaacttctt cacaaagaca cctcgaatta attagactct tgtgttcaat
     3241 tttatcacta aatgccgtga aacaaaaggt aagttgctcc tcttaagctt ttttttagca
     3301 aataatagat tctgtcattt ttagcctgaa agacaaattt ttttatttgt ttatggatca
     3361 atctttgctt tgaataactg caatcatacc atctgaaaaa gaaaattctg cggtatctga
     3421 aagaaattcg ttacttgcat aagatgtcgg atattctact tctttatttt ccaatacata
     3481 cttgctgcct tttaatgtaa gattcgagac gggagtcaag cagcaataag ctaaatactt
     3541 catatctggt atctgtctta ctatatgctc tccaggcaaa taataagaca aataattttg
     3601 acaatctctg ataataagct gatgggcaaa gggtttgaag cgaggttcca tacctaacca
     3661 gagattggct aaaagatgat ccaatcttcc gcccgtagct ccaatcaaag tgacttccgc
     3721 ttgcgggtaa cgttcaaatg tttttaagat acctaactgt gtatctgtat cgtctttttc
     3781 tgcaggggaa cgggtatact ctttggcttt tgcaaatact tccttctgct cttcgttcga
     3841 caatgaatca aaatcaccca ctgccagatc tactgggtag cctgcttgaa gcaggtacag
     3901 cgcaccacgg tctatcccga cataggcatc gtatttggac agcagagact gcggccagtt
     3961 ttgcggatta cctccagcag ctaataaaat tttcatttag tctaatgcct cttttaatcg
     4021 ttcgatttgt ccgactggat tttcggctcc atagatatat gatcctgcaa cgaagacatc
     4081 cgcccctgca tctttacaga tttttgctgt ttctggaacg attccgccat ctacttcgat
     4141 tgcataagtc caattgttct ctctacgtag atcagcgagt tccgtaattt tttctactgt
     4201 ttcttcaata aagctttgtc cgccgaaccc aggattgaca gtcatgacta gtacttgatc
     4261 agccaaaggc aaaacatgtt tgatcatgga gactggtgtt cctggattga taactactcc
     4321 agctttaact cctaagcttt ttgtcatttg aagtgcccga tgaatatgag gagttgcttc
     4381 gacgtggaca gtaatgatat ctgctccagc ttttgcaaat gctggaatgt agttttctgg
     4441 atcaacgatc atcagatgga cgtctaacgg taatttagta acaggacgga ttgcctgaac
     4501 aatatttggc cctaatgtga tatttgggac aaatcggcca tccattacat ctacgtggat
     4561 gtaatctgcg cctccttttt ccactaactc aatgtctctt tgtaaattgg caaaatctgc
     4621 actcaaaatg gatggtgcta tcttcattct tgtttcctcc tattttcgtt taccttcttt
     4681 ttttgcatac attggttttc ttttttcgat ttcttgtaag aattgcaaat aattgtcgta
     4741 acgtgtctgt gcgataccac ctgtttctac tctatgtttt acttcgcagc caggttcttt
     4801 agcatgcatg cactctctaa atttgcaatg gacagaagct tctacaaatt caggaaattg
     4861 tctcggcagt tctcctgctt caattgtcaa aaagtcaatc gagctaaatc ctggtgtatc
     4921 tgctactaac ccgccataga taggaatcag ttccacatga cgtgttgtat gccgtcctct
     4981 tccaagtgca tcagaaatct ctcctgttgc taaattcagt tccggtgata tttgattcag
     5041 tagcgtcgat tttcctgctc cagactgacc cataaagacc gatagccgtt ctgggaagta
     5101 acggatcagt tctgtcgtat cacccgtatg attgggtaca atcaccggat agcctatttt
     5161 agtataggtt tgcttgatct cttcgatttt ttctgggtcg tctgacaagt cggtctttgt
     5221 caaaaaaata atcggttcga tcgactcgta ttccaaggta actagaaagc ggtcaagtag
     5281 attataagaa aatgcaggct ctacgatact tgtgacaatg attccaagat caacatttgt
     5341 gactggtggg cgaactaatt gattttttct cggcaagatt tccagaagat agccgtctgt
     5401 ttgattttca ctctcaaaga tcacctgatc tccgacaagc ggcgtgattt tccgattccg
     5461 gaaatttcct cttcctcttg tttgataggt ctctccgttt gcgtatacat agtaaaaacc
     5521 gctgattgct tttctgattt gtcctttgat aagtgccacc tccgtttttg tttattttac
     5581 catacgaaga ggaaagtagg tgttattcca tgcaaacttc tcaaagtata tggcttgaaa
     5641 attgatcaaa agagatagaa acacaaaaaa gagaatgcaa caaatcaatg tcgcattccc
     5701 ttctgatata ttttattcag cacttatttt gaactttctg aggtgctagc tgatgaactt
     5761 gtggagctgg aagattcttc ttttgaagag ttgcttgtgc tactggagct acttgagctg
     5821 ctagaactgt ctgaagagtc tttttcagaa ctagaagacg ctggatctgg tcctttagag
     5881 taaattacgt tcacaaccat tcctttggta attgtcgttc ctgcccctgg tactgtacga
     5941 atcactttat ctttatccac cgtgtttgaa tattcggaag tctcatgccc gatatattct
     6001 gcaccgacac tagctaaata actttgtgct tcgctttttg tatagccgga gatatcattc
     6061 aatttaggct ctgttccttg gcttacgaca aaattgatgt aagtttcttc tgctacaact
     6121 tcacccgagg ctggatcctg actgatgatc gaacctgatg gtacagtgtc gctatagtca
     6181 taagtagcac taatattatt gatattgaat ccttgtgtaa caagccaatt acgaacagtt
     6241 tcgtagtttt ctcctacatg actactcaaa gtcactgttt ctggtccttt gctgactgtg
     6301 aaagtaatcg tgtctgtttt aggatcgact tctgtatctt ctgatggaga ttgcgaaatg
     6361 atcattccgc tttctacctc tgaactgaat tcctcttctt tttgaatcaa gtcagcagaa
     6421 aaccctaatt ctttcaaact tgcttcggca tcttcataac ttttaccact gtaatcatct
     6481 aatttgattt ttttcgtgcc gctactaata tacagagtga cttcgcgttt ttgcttgact
     6541 gtcgttcccg ctgccgggtc tgtcttcact acattacctt cttcgatttt gtcatctgca
     6601 atttcttctg tttttgttgc tacttctaat ttcgcttttg cgagtgtttc tctagcagaa
     6661 gcttcagaaa gatcagaaac atcaggaaca acaacttctc ctcgcccgcc agaagcaaag
     6721 aatacagctg ttccgattcc taaaacagca agaatagcta ataggatcag ccaccatttt
     6781 ttctgtcctt tctttttagg atcaggcaga acttcttcct cctctggtaa ggtttcgttt
     6841 tctggcttgt cttttggcag aggcatagat ttgaatgaat caggcattgg tgcatcttcc
     6901 gtaaggggtg tgataacttt tgtttcatta tccatcacat gaggctgcca tttttcttcg
     6961 tttgcacgag ctggtgataa tactgtagaa agatcatcag acatttcctc ggctgactta
     7021 taacggtctg caggttcttt agccgttgcc cgtaaaatca cgttttccag tgcttgtggc
     7081 acatttggat caagtgcttt tatagatgga agatcatctt gaaaatgttt taaagcaatt
     7141 gttacagcag attcaccatc aaacggtacg ctacccgtca acatctcata aaggatgatc
     7201 cctaaagcat aaatatctga ctgcttcgtt gccatacttc ctctggcctg ctcaggggac
     7261 agataatgaa cagatcctag catcgtattc gtctgcgtga tcgatgtttc agataaagcg
     7321 atagcaatcc cgaaatccgt gatttttaca acaccttcat tatcgatcag tacgttttgg
     7381 ggtttcaaat ctcggtggat gatttgatgg ttatgagcta aagaaatagc agaaaggatc
     7441 tgctgcataa tatttactgc tgtttcataa ggaacaggat aatgtgtttg gatatatcgt
     7501 ttcaagtcca ttcctttgac gtattccatc accagatact gcatgttgtc ttcttcacca
     7561 acatcgtaaa cactaacaat gtttggatga acgagttctg ttgctgctaa agcttctctc
     7621 tgaaatcgtc gaatcgcagt ctggtcattt tgaaagtcaa atcgtaagac tttaactgct
     7681 acatcacgat ccaagattaa atcgtgcgcc aaaaatacat ttgccatacc accgctaccg
     7741 atatttccag tgatttgata acggccattt aattttttgc ccaactcgat catacactgt
     7801 cctccttata ttcgataaga agaacggtga tattatccgc tccgcctgct tcattggcac
     7861 gatcaatcag ctcagttact ttatgagaca atgggccttc ttgattgatg atcgttccta
     7921 tttcttcttc agacagcatg ttcgtcaagc catctgaaca taacagcagt cgatctttta
     7981 gttgccaaag atgagtagaa acatctactt cgactgttcc aggcatgccg acagaacgtg
     8041 ttaaaatatt tttccttgga tggttcactg ccatttcttc gctgatttct cctgatttga
     8101 ctagttcatt gactaaagaa tgatcctccg tcaattgcat gattttatct tcacgaatca
     8161 aataagctct gctatcccca acattggcaa tcgtgaacca gtcatctgac agagcggccg
     8221 ctacgattgt tgttcccata ccgttatatt ccggctgttc ttgtccacgc tgataaatca
     8281 gcatattttc ttcttgaatc gattgaatga accattgggc gattttttca tcgtcaacag
     8341 cttcttgttt ttcccaagct tcgcctagat tcgttaccgc catctgactg gcaatgtctc
     8401 cggcacgatg accacccatc ccgtctgcta atacagcaag cttgagacct gaacggctgg
     8461 taaatacact ggcatagtct tgatttgtgt tacgtcgtct tcctacatct gattgatatt
     8521 caatctgcat tttttcacct cattactttt tgcgcaaaca acaaatgaaa aatccatctg
     8581 tgtaaaactg atgcggatac aaagtcaaca ttttctcttc gattgatgat tgtagcatag
     8641 gattggtcaa aacatcaatc ctctcaaaat cagggtgtct ttccaagaaa gcagaaacaa
     8701 cgtcttggtt ttcttctttt aagatcgtac aagtgctata agtcagaata ccggaagact
     8761 ttaaagttgg tgcgcagctt tctaaaattt ccaattggat ttttggtaaa ttcgctaatt
     8821 catccggctg ttttttataa cgaatatctg gttttcgccg aagcagccca agacctgaac
     8881 aaggcgcgtc tactaaaatc cgatcaaact gctctgcttc gaactgttta ctgacttctc
     8941 tagcatccat cttttgagcg tagacagcag aagagacacc tagacgtgct gcattatctt
     9001 caatgagttt gactttgtgt tcgtgtacat ctaaagcgac cacttctcca ccgcatgacg
     9061 gatctaaaaa agaagcaata tgcgtcgtct tacctccggg tgctgcacag gcatctaaga
     9121 catgatgttc aggctcgatt tgcatagacg gcgctacaag catcgaactt tcatcttgga
     9181 tcgtcatgac gccatcgtgg aacaatgagc tagaagctaa atgccctttt ttcgctacta
     9241 taccgtatgg actgacttgg cttttttcag ctgtgatctc ttcttcttgc aaaacagtga
     9301 tggcctgatc tctggaaagt cggcgtgtat ctactcgggc actagcatgg ctaggctgat
     9361 atagcgataa accgagcttt tcagtttctt cgtatccgat ttgatccacc agtcgtttcg
     9421 tcatccatag aggcaggctg attttgacag aaagtctttc gataggatca gcaatgcctt
     9481 gcagatctgg tgctccttga cgctgataat tcctcaagac accatttaca aatttccctg
     9541 tcccaggatt tccttttact tttgcgattt cgaccgcctc gtttaaaata gcatgtgcag
     9601 ggactttatc taaaaattct aattggtata cagaaagata aagaagcatc ttgacccagt
     9661 catccacttt ttttgcttta gcgatcaatg gtgcaacgta aaattccaat agtcgctgat
     9721 ggctaatcgt accatagatc aattctgtaa ataaacccac atctttttga tttagctcgc
     9781 tttgtttgat catctcattc aacaaaagat tcgagtaggc gccacccttg ttcactcgct
     9841 ctaatgtcag taatgccata tatcgtactg attttttcat ttttttaggg atttttttca
     9901 ttatttcacc tgctgaccaa tctgaacttg ctgaccgctt ccgttaagga aatcatgtac
     9961 ggcctgtttt cctttaccag ctggctgtaa ttcattgatg gctaagacgg tctgtttacc
    10021 gcaagcaatc catagatttt ttttgtcttt ccggatgatc gtaccaggtg ctttgtctgt
    10081 agtttctgcc aatacttcaa ccgataataa tttccagcgt atgccctcat aggtcgtgaa
    10141 tgcaattggc caaggacgca ttccgcgtac ttggttgtca atttcttctg cagttttatg
    10201 ccaatcgatt gcttcttgtt cccgtgtgat gtttggcgaa aaagttgctt ctgactcgtc
    10261 ttgaggtact ggtttttgtc catccaaaat attgggcaat gtttctagca atagctttct
    10321 tcctaaaagg ctaagctttt caaacatcgt ccctacatca tcttgtttag tgattggtat
    10381 gctttcttgc gcatagattc cgcctgcatc catttttttg atcatttcca tgatcgttac
    10441 accagtttct ttctccccat tcatgatggc ataatgaaca ggcgcacctc cccgatattt
    10501 aggaagtaaa gaagcatgga cattgattgc ccctaatttt ggtacttgca ataacttttc
    10561 aggcaagaat tggccaaatg ccgcagtgac gatcacatca ggcaccaatt caatgatttc
    10621 ttccatttct ggcgaaccgg agattttctc tggctgcagc acacggatac cgtgcttcag
    10681 agccgctttt tttaccggtg tcggcgtaat tactcgtttc cgcccaactg gacggtcggg
    10741 ctgtgttacc actgcctgga tctcgtaacc tgattctatc agcccttcaa gaatcggcac
    10801 agaaaaagca ggtgtgccca taaatactag cttagtcatc aagatgttcc tccatatatt
    10861 cttcaagttc ttcttccggg atctgctgta tcattttctc aatgaacaag ataccgtcta
    10921 agtgatcgat ttcatgctgg aaggcacggg ctaaatagcc aaaagccgtc acttctatct
    10981 cttctccgtc tcgatcataa taacgcacgg tcacttcatc tgctcgcttg accgttccat
    11041 agacatgcgg aatactgaga caaccttcta cgtcaaggct ttcacctttt gcttcgatga
    11101 tttctgggtt aatcagttca aatttctcgc cttcatctac ttcgatcaca gcaattctct
    11161 tgttttgtcc tacttgagga gcagcgatcc ctattccgtc attcgcaatc attgtctcat
    11221 ataaattgtc taataaagcg attgtctcgt ccgtgattac atcgattggc tgagcagttc
    11281 tttttaattt atcattaggg tggatcaata taggatagcg cataatttgg ccctttctta
    11341 aataaagttt aatggttcgt tgtcaattgc cacaaaaaga ccttgtcgct gttctttctg
    11401 gcttccattc aaaatttcat ccaaaacatg aggaagattc ggttcatgtt tatatttgat
    11461 gattagttga taataatatc ggtttttcac tctcaaaatc atgctgggcg ttggccctaa
    11521 tagcaagctt tgagggctta acacttcttt tagctgattg gcaatctgaa agattttctt
    11581 tgccgctact tgttcttcag gatggctggc ggttattttc acagtaaagt aataaggagg
    11641 atagcctcca cgatgccgta catgcatttc ctgctgataa aactgctcat aattttgttt
    11701 tttagccaat tctatggcgt aatgttgagg attgaatgtt tggatcacga cttcgcctgc
    11761 tttttccgct cgccctgctc gtcctgaaac ttgggtaagc aattggaatg tatgctcact
    11821 agaacgaaaa tccggtaaat tcagggaggt atcagcattc aacaccccaa ctaatgtcac
    11881 ttctggaaag tccaaacctt tagcaatcat ttgtgtccct aataatatat ctgcttcttt
    11941 tgcaccgaat ttttgtaaaa tctgttcgtg tgcccctttt cttcgagtgg tatctacatc
    12001 catacgcaaa atcctagctt gcggataaag ttctcggagt tcctcttcta ctttttgagt
    12061 acctgttcca tagtagcgga ttttattgcc gccacaatta ggacagcgat tgggaatagg
    12121 ttcttcatgt ccgcaataat ggcagcgcat tgaacgtgta tccatatgca gtgtaagaga
    12181 gatatcgcaa ttaggacatg gaagaacaaa tccacagtcc ctacacatca caaaagacga
    12241 atatcctcga cgattgagca gtaggacagt ttgttctttt ttgtctaatc gattttgtat
    12301 tttttcctgc aaattagcag aaaaagtaga ggtccgatga ttctgaaact cttcacgcat
    12361 atctacgatc tcaatagctg gaagctgtgc agctgctttg gctcggtggt ttaactgcaa
    12421 taactgataa acgccttttt gagctcgtgc tcttgattct aaagagggcg tcgcgcttcc
    12481 tagaactatt gggcaatgat gatatttact gcgccaaatt gctagatctc tcgcatgata
    12541 acgcggtgtc tcatcttgtt tgtaacttga ttcatgttct tcatcgataa tgatcagccc
    12601 gatatttttc agcggggcaa aaatagcaga tcgtgcgcct actactactt gcgcttcttc
    12661 ccgctcaatc ttgcgccatt catcatattt ttcaccttgg gaaagtccgc tatgtaagac
    12721 cgcaactgat tctccgaagc gtcctttgaa tcgctcgacc atttgcggag tcaatgcgat
    12781 ttcaggcact aacataatag ccgttttatt ttctgacaaa acatcagcga ttgcctgaag
    12841 atatacttct gttttcccgc tgccagtgat tccctccagc aaaaagacct gatctttttg
    12901 ctgctgacct gctgtgatga tttgttcgac agcatttttt tgttctgcat tcagttccaa
    12961 tgccgtcgtt tggtcaaata ctcggtcttt ataagggtcg cgataacgtt ctgattctat
    13021 aaactccagc cagccctttt cttttccttg attcaagaca gcagtactga ttcctttatt
    13081 tttgaaataa gcaattggtt cttcttggct cagttcagat aaacacaaaa gaagttcttt
    13141 ttgtttcttg gcattttgcc gtaattttgc ccattcttct tcgatttgct tttcagttaa
    13201 caaagaacgg accaaacgaa tcgtttttac tttatttcgt gtatggactt catatttgat
    13261 atctactaat tgctcttggc gccagcgaat caattgagaa aggctgcctt cttcttctgc
    13321 ttccttccag gaaatcactt ctcgctgatc aaacaatacc tgaacttctg gtgtgtctgc
    13381 caaaggatag atcaatttat catattctgc tttcatcaca ctcggaagca tcgtttgtag
    13441 acaagtgatt ttgaatgcgt aagtaatttt tttcatataa tcagctaatg aaagtaattc
    13501 ttcattgacc accggagcaa ggtctagcaa acgaataact gccttcaaag atggattagc
    13561 tgattcttct gatttttgga tagccataac aaatccttgg acatgacgat tgccattacc
    13621 aaaagggact tctactcgca tcccgacttg caaagctgtt tccatttcag agggaaccaa
    13681 ataagtgaat ggctgatcgg tctgcatagt aggtacgtcc acaatgactt cagctgttaa
    13741 tgtcatggtt cgtttcctcc tttttttgtc ttttcttatc aagcgacaaa aagttccttg
    13801 cctattttac tagagatcga tggatttgtc ttatcttctg ccaaatccta acaaaaaatt
    13861 aacagcagat aagaaaaaaa agaactgtgt caaaaagttc agacacagcc ttttctacca
    13921 gacaataagt ggcgaatgtt tgaaaaggaa acagctgggt ttgcttttct ttttagatgt
    13981 tcgctctatt tttctttatt acattttttg ctcttcacgg atacgtgatt ccaattcgtg
    14041 ttgttcacgc tctttttgtg cttgacgttc ctcttcttcc atcttcaaac gttcacgttt
    14101 taattcagga tgtggatcat tgactacatt tcctgcatcg atttcttcta aagcttgacc
    14161 gacgcttttt actgaatcaa aagaatctag agtaggctgt gcacttgcat ccaattcatg
    14221 cgcgcgttta cttgctaaaa tcactagaga atattttgaa tttacacgat ctaatagtga
    14281 gtcaattgaa ggttttaaca tcataagact acatctcctt taacatttta atatatttac
    14341 cgattacgcg atctactcga aaatgttcac tggcaataat gtttttgatt cgttcaactg
    14401 ctagcggcac ttcatcattt acaacagcat agtcgtaaag agccatcatt tcgatttctt
    14461 cttttgcaac tttcattcgt ttttcgatta cttcatctgc atctgttcct cggcctacga
    14521 tccgtgattt taattccgct aaatctggcg gagtcaaaaa gatgaataca ccatcgggca
    14581 ctttttcttt gacttgtttt gctccttgga cttcaatctc caagaaaaca tcttttcctt
    14641 catccaatgt cttgttgaca tatgacaaag gagttccata ataattcccg acatattctg
    14701 cgtattccag catctctcca gcttcgatca ttgattcaaa ctcttctttg cttcggaaga
    14761 aataatcaac gccttcgacc tccccttcac gcattttacg ggtagtcatt gagatcgaat
    14821 actgaaaatc attatcctca ctctcaaaaa ttgcttttcg tactgtgcct tttcctacac
    14881 cggatggacc agacaacacg attaataatc cacgctctga catgacgacg tcctttcaac
    14941 tagtcttttt gtacttatcg tttattttgc actttttttc tgcaactttc aaggttatct
    15001 ttaattatag ctagaaattg gtaaaaaagc taccctgtac caagaggtcg taaaaaagct
    15061 gtcctgcatc aagtaataag aacagccaaa cataaacagc ttctcatgtt tttcgtttgg
    15121 ttggggttat tatcgcagat gcaagctttt gaacgccgtg tataagaggt cgtgaagaag
    15181 ccgattagtt tcgagtatga agaaaaattt tgtgaaaata gtttctcaca tttttgcata
    15241 aatttggctt aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga
    15301 ttgtgacacg aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga
    15361 agaaatccaa ttctgtttat ttattgtatt tctttgttca gcatccttgc taaaagccca
    15421 tattggacaa agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg
    15481 tcggatcttc tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt
    15541 aagcaccgtc gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt
    15601 tccatacgtc aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac
    15661 ttaattcgtg gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat
    15721 attttgtact tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt
    15781 aatttcgatt gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat
    15841 caggaaatct aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg
    15901 tacatggatc gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc
    15961 tagctccgtc aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt
    16021 ttgatacctt atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag
    16081 tacttctttg aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt
    16141 ttctcgactc gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc
    16201 caattcttcc atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcac
    16261 gaagcgttcc gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat
    16321 tccgtctgtc aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt
    16381 ttcgtttttg ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg
    16441 ctgtttaata agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat
    16501 ttgaacatct agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt
    16561 tgtttcgcgg atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc
    16621 attacatatt ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc
    16681 ctttccccat tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac
    16741 cattccagct gactaggttc agttctttac gccacccctt gttgttttct gacaatatag
    16801 cgatttcttc tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata
    16861 atataaatat aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg
    16921 atccttgcgt tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg
    16981 tattttttgc tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg
    17041 tgccaataaa tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat
    17101 gtctacttga tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat
    17161 cgccaatccg catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca
    17221 aaattttatg cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa
    17281 aaatcctcgc cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata
    17341 gtaatagcct aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct
    17401 ttggctatca aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat
    17461 aaaaatgtct ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa
    17521 tgcctctgtt gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc
    17581 gtctgcatat ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga
    17641 ggcgagtatc caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc
    17701 cgctagttct ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag
    17761 taaggacaaa tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc
    17821 ttcaattagt gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc
    17881 ttcaactgca gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa
    17941 attcacagct tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga
    18001 ggtacctgtg ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat
    18061 ctgataagca ccgtgcgctc ctcctctacc taaaacaagt gctgtatgat agaccagctt
    18121 tttatttaat tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc
    18181 tgatagagga gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc
    18241 ttccatatac cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt
    18301 aaaaataaca tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac
    18361 tgctttaaaa gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg
    18421 actttgaaaa aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt
    18481 ctgtgaaaag taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag
    18541 tacgtcataa aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat
    18601 tcataaaacc tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga
    18661 gtgttttcaa tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt
    18721 ttaatgcaaa tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga
    18781 ctgagtcagt ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga
    18841 ctgctactga ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc
    18901 catctgcttc tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag
    18961 aaaatattca gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt
    19021 ggatcgactt tactgattta acgatgctga aagaattgac acctttagaa atttcagaat
    19081 tattatattt tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga
    19141 ataattttgt atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag
    19201 atgaatttta taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg
    19261 taaaaaaatc ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga
    19321 aagaaatgcg taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga
    19381 tcgatggtaa atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag
    19441 gcacccggta tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac
    19501 actggcaatt ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct
    19561 tatcaaaaaa taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct
    19621 tcttttttat ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt
    19681 ggatccctaa taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca
    19741 ttacgaaaga aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca
    19801 ctcctgcaat aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga
    19861 tgctttttct aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca
    19921 tgagccctgc tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga
    19981 cacttaaata ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca
    20041 gcatccctgc ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg
    20101 aaatagaaaa actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg
    20161 caacaatcgt tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga
    20221 ataaaaaagt cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac
    20281 aattcctacg ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca
    20341 gcccaattgg actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg
    20401 actaactggt ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact
    20461 tacttcatcg attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac
    20521 tagacgctcg atagctgaat cttctattct ttgtaaagca gggccggata atactggatg
    20581 tgtacatgaa gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt
    20641 gatcgtacct gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg
    20701 tccaatgata ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc
    20761 aatcggagat ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg
    20821 tgaaacaacg acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag
    20881 tggcgcaccc atcaaatgat cgactggaat atcaaagaaa ccttggattt ggactgcatg
    20941 aaggtccaaa gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt
    21001 cgctgtgatt ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg
    21061 catgactacg ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa
    21121 ctccatcaag ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc
    21181 acgaatactt tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga
    21241 ttttcctaac tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt
    21301 taacgcaaaa atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt
    21361 ctttttcaca actctagaat tctacctaat aaatagtagt catatttttt aaataaatca
    21421 agtgattttg caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt
    21481 gacttgtctt gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga
    21541 cccggcagca atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga
    21601 accaataaag ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac
    21661 gaagacaaca ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt
    21721 caagtgaccg actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc
    21781 gatatggacg ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc
    21841 tgcatgtttc ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc
    21901 aatcctactg tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt
    21961 atttccttgg atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc
    22021 atcgatgtat gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt
    22081 aattcgcttt ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat
    22141 tgattcttca aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat
    22201 ttcgatgata tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa
    22261 cgcatcgaac aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag
    22321 agcttcttct ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc
    22381 acggatgatt cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact
    22441 tgcatttttt ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg
    22501 tgtatcgcca ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg
    22561 caaaacagca tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc
    22621 tagttgtgct ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt
    22681 cgtttcactg acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa
    22741 aactttatat aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata
    22801 acgtgcgtcc aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat
    22861 acccattttt tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa
    22921 atagatagac cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg
    22981 gtcacaagtc acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga
    23041 cttagagtta aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct
    23101 tcggtattag ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa
    23161 tactcaggac aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt
    23221 ctcggtatta agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt
    23281 actactcgaa actaagcggc tttttcacaa cctcttattc tgaaaaataa tttcctggca
    23341 caaccgtaat atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat
    23401 catcgatggc gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt
    23461 ctgattcgaa ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga
    23521 aatcatccac tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct
    23581 tttcaattct ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta
    23641 tttttgagtc ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg
    23701 caataggtac gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt
    23761 tagaagcaat gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga
    23821 gataaacata tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt
    23881 caatatattt tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag
    23941 cacccgggat tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc
    24001 ccaaatcttc actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg
    24061 aaatcaattc atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc
    24121 gacgtacttt cacctattcc aactcctttt aagaaatttt atcgttttac attatataag
    24181 tttttcttca aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta
    24241 aaataaaaag aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt
    24301 tagttatttt ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac
    24361 aaagcaataa agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta
    24421 gttagtcctg acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg
    24481 aagcttttgc ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa
    24541 agtgcaccgg caatcgggat catcacagca atagccacac cagtaatcac attgaacatc
    24601 atcgacatca aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga
    24661 acatacatcg gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct
    24721 aaaaagatga cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata
    24781 cttgttgctg aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca
    24841 gccattaaaa tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc
    24901 aatatcacag ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa
    24961 aagaatccta acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat
    25021 tgtcttctga tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg
    25081 gcaagaaacg ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc
    25141 tcaccagtcg gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca
    25201 ttagaactga tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc
    25261 tggattcttc atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag
    25321 caaaaatacg cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc
    25381 gatttcgcat atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt
    25441 tttcagagag acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga
    25501 tcaccgtact aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac
    25561 ttaatttttt tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca
    25621 gtaatcctaa cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa
    25681 tgacaaattc acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat
    25741 agtaaaaggt caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact
    25801 taattgatac tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg
    25861 cctgctttga tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact
    25921 cctgtttcat tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc
    25981 actttgatgt tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt
    26041 tcctgatctg gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa
    26101 taagcaaaag cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg
    26161 gagtattttt gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa
    26221 gtttcctttt tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt
    26281 atagctaata ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct
    26341 tcttctgatt cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct
    26401 gcttcgatca tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat
    26461 tccatgtctg aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt
    26521 gcacttggtt cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct
    26581 tcatccccta cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc
    26641 aaatcctctt ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca
    26701 cctatcatta ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac
    26761 aaatcggaat tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc
    26821 aatagaaatc catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg
    26881 atgagttgct gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt
    26941 aggattcctc agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac
    27001 taaataaact tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata
    27061 atgttgacac aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc
    27121 gtacttgatc atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc
    27181 ttctaaactg tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc
    27241 agcaagtccg tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt
    27301 acgtgtagag acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg
    27361 taatacttgg atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta
    27421 aggaacatct gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag
    27481 attccataat tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa
    27541 tccagctgct actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta
    27601 tttatttttt agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa
    27661 tgctttattt gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc
    27721 tcccaagtct acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata
    27781 taatacatcc agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc
    27841 acccattcga aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct
    27901 tatgtctgcc tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt
    27961 cataatccaa acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact
    28021 cggaacattt cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa
    28081 attacttcct ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt
    28141 cttttcataa gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat
    28201 gactttttct tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc
    28261 tagattggct tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata
    28321 gtcagctaat tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt
    28381 cttcgtcatc ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc
    28441 gacaccgcta gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg
    28501 tgcaccttga agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag
    28561 ttgatcgtac caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg
    28621 actcagccga gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt
    28681 gaagtgaaaa cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc
    28741 aagctgatag cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat
    28801 tttaggattg ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc
    28861 aaaaacgact tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg
    28921 aagctgatgg cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt
    28981 ttcgtactca ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa
    29041 agctagcaat gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa
    29101 caaaataaaa tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc
    29161 aattgcgtaa acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca
    29221 agaataaacg aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat
    29281 caagacatat accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc
    29341 aaaagatagt aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct
    29401 agcaaggccg agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa
    29461 tgagaacggc aataatactt gaaatagccg actgattgca gacagattag tgattgttga
    29521 tagaagtacc atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac
    29581 cttttggaaa aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg
    29641 tgtgatgaac aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata
    29701 taatagccaa actactgcta catcttgccg aacagacaat tgactcattc ctaagatcat
    29761 caaaacgtca aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc
    29821 aatcatgaac ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat
    29881 gatcaaaaat accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag
    29941 caagcctttt ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa
    30001 aatgactggt gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact
    30061 ttctgactga ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt
    30121 cagcataaat agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt
    30181 tccataaaaa ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt
    30241 gatcgtgttt gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc
    30301 ttgtttttct aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag
    30361 cagtccagcc agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca
    30421 aatatcttgc caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa
    30481 cacggagccg aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat
    30541 caagtatcgt ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc
    30601 ccaatgttat cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga
    30661 tagaaaaaga aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg
    30721 gcacagggaa tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga
    30781 aagaagaggt gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa
    30841 ctggcgtcca tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt
    30901 tagccgtgaa accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga
    30961 aagtcttatt catttctatt gcttcaggaa aatcattaat agatttagaa acagcgcttg
    31021 aagatcctca ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag
    31081 gcgtttctgc gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga
    31141 tgatttttga agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca
    31201 ttggccttgc tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga
    31261 ctggtgtact gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta
    31321 tgggttcttg tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag
    31381 tttcttctcc tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca
    31441 tcccagcaat catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata
    31501 aaaaataatg atagcaatgt tttttcgtgc cttacaaaaa aagaagagct gtggcttttg
    31561 ccgcggctct tcttttgtat tttttattat ttagcataat cagttgcacg agtttcccga
    31621 ataacgatta ctttgatgtg tccaggataa tccagttctt cttcgattcg tttacgaata
    31681 tcacgtacta aacgaacagc gtctaaatcc gagatttctt ctggcttgac cattacccga
    31741 acttcgcgtc cagcttgaac tgcgtaactt gattctacac cggcgaagct gttcgaaata
    31801 ttttccagac ttcttaaacg acggatgtag ttttctaatg attcacttcg cgcaccaggt
    31861 cttgctgctg agagcgcatc tgctgcagct acaaggacag agataacaga agtagcttcc
    31921 acatctccat gatgagaagc gatcgcgttg attactactg ggttttcttt gtacttggca
    31981 gctaattccg caccgatctc aacgtgagat ccttcaatct catggtcaag tgctttccca
    32041 atatcatgga gcaacccggc acgttttgct aattggatat cttcgccaag ctctgctgct
    32101 aagataccag ctaattttgc aacctcgacc gaatgcttca agacgttttg tccataacta
    32161 gttctgaatc gcagacgtcc gatgatcttg attagatctg gatgaagtgt atgagcaccg
    32221 acttcaaacg ctgcctcttc cccgtattcg cgaatacgtt catccatttc tttacgagat
    32281 ttctcgacca tttcttcgat acgcgctgga tggattcgtc catcttgtat cagtttttcc
    32341 aaggtcatcc gtgcgatttc tcttcggatt ggatcgaatc cggaaagaac aactgcttcc
    32401 ggtgtatcat cgatgattaa atcgatacca gtcagcgtct caagcgttcg aatattacga
    32461 ccttctcgtc caatgatacg acctttcatt tcatcattcg gaagggtaac gactgaaaca
    32521 gtcgtttctg aaacttgatc tgctgcgcag cgttgaatcg ctaatgataa taagtttttc
    32581 gctttgcgat ctgcttcttc tttggcacgc tgttcagatt cttttaccat tagagttaac
    32641 tcatgattca gctcttcttc agtcgatttc atgatgacat ctcgtgcgtc ctcttttgat
    32701 aaaccagcaa ttttttctag ttctttttgt tgatcttcaa tcattttttc aacgtctttt
    32761 tctcgctctt caattaattg ctgttttgaa ctaagtgctt cttctttact ttcaagtgag
    32821 ttctcgcgtt tttctaacga gtcatcttta cgattcagcg tttgttcgcg ttgaatcaaa
    32881 cgattctctt gagattttaa ttctaactta ctttctctca gctcactttc aacttcagaa
    32941 cggtatttct gattttcttc cttagcttct aacaatgctt ctttttttag agtttctgct
    33001 tctttttttg cactatcgat gattccagca gctgatgatt gtgcaccagc tatttctttc
    33061 tcgtgacgtg actttgcgac cataaaccct aaaccaagac cgacaattaa accgatgata
    33121 gcgaggagaa tgttgaatac cataaaatcc acctccatac tatctttttc atttttatca
    33181 tctatacagc ttctatgata aattattcta aactcgtcaa tatgcatctt cgcatatatg
    33241 tgtacaaaaa acatgcacaa ctttattcta atgtttgtac cccgtagtgt caactttaaa
    33301 taaacaaaga aacggctttc tacttattca ttaaacgatg gtttttaaaa taaatgacac
    33361 tagtttcata taggtaaacc tttaaattga gaaacttttc tattaaatta ttctttgtag
    33421 atgaacagat atttttattt tatgtaccca ctcgtataat taaataactc acgatttctt
    33481 tattcaataa aaaaacggca ggtcaaggag agtacctact cttcagacca gccgtttttg
    33541 tttatcataa tttttattct tctactaaag gcaattcttc ttgtcctttt tcatcttctg
    33601 ggacagcgac ttcttcacca ataccataag ctgctctaac tagagcagaa acttctgcca
    33661 tcatttcagg atgattagcc atatagattt tagcgttttc ccgtccttga ccaatccgat
    33721 cttctttata agaataccaa gcaccgcttt tatcaacaat gtctttttct actgccatgt
    33781 ctagaagttc tccttcttgt gagatcccta aaccatacat gacatctact tcagcgattt
    33841 tgaaaggagg tgctactttg ttttttacga ctttgatctt tgtacggtta ccaacaatat
    33901 ctgtaccttg tttcaattgt tctgcacgac gtacttccaa acggatcgtt gcgtagaatt
    33961 tcaatgcgcg tcctccagga gtgatttctg gattcccaaa catcacgcct actttttcac
    34021 ggatttggtt gatgaaaata gcgattgttt ttgttttgtt gatcgaacca gagagtttac
    34081 gcaatgcttg agacatcaaa cgtgcttgta acccgacatg tgagtcaccc atttcgccgt
    34141 cgatttctgc tcgtggaact aaagcagcaa ctgaatcgac tactacgata tctacagccc
    34201 cacttgatac taaagcatca gcgatctcta gaccttgttc tcctgtgtca ggctgtgaaa
    34261 gaagtagttc atcgatattc acacctaatt tttgtgcata ttgcggatct aacgcatgct
    34321 cagcatcaat gaaagcggcc gttccgccat ttttttgtac ttctgcaata gcgtgtagtg
    34381 caactgttgt tttacctgaa ctttcaggtc catatacttc gatgatacgt ccacgggggt
    34441 aaccgccgac acctaaagcg acatctaacg caagagagcc acttggaatt gtagagattt
    34501 gttggtcgat tttttcgccc aatttcataa tagagccttt accatagctc ttttcaattt
    34561 ttttcagtgc agcatctaag gctgctttac gatcatctgc caatacgata atcctcctta
    34621 tatttacaaa tcagagcaac atttcattat ccttatcata tcttttttag tgaaaaaaag
    34681 caagaaaaaa ccgaacaaat attcgcattt atcattttct taaaagctgt tttctcaaaa
    34741 gatcaaatcc ttgcataacc gcattatatc tgatagcagc acgatctctg gcaaagtgat
    34801 atttttccga aaccacgccg tctttagatg aaatagcgat ccatactgtt cctgcagatt
    34861 gaccttctaa cgtgtccggt ccagccactc cggtgaaagc tacactgtaa tctgttcttg
    34921 ctatctcacg tgcttgaatg gccatctgct ccacacattc tttgctgact gttccatatt
    34981 ctgctaatag ctccggatca atctccagaa aaccagcttt tgtttggagc gagtaggtaa
    35041 cgaatccgcc tggaaatact tcagatacac cagcaatgtc gcctaatgcg gcttggaagg
    35101 cgccggctgt caaactttcc gctgctgtta cagtctgctt gttttctttc agtagttcca
    35161 ctacgacttt tgctagcgaa ttatcatcgc cgtatccata aaaatattcc cctactcgtt
    35221 cttggatttt ttcttctaat gctaaaagag cttgattccc tgcctgaaca tcatttgttt
    35281 tcactgtcag tcgtaatgtc acttcatttg gtttggcata tggtgcgatc gttggattga
    35341 tctgtgtctc aatcagatct tttagctcag tgaccagtcg cgattcacca atcccataaa
    35401 agcgcaaaac tttagaaatt agtttttctt cagaaggaaa acgttcctct agtaaaggac
    35461 gtacttgttc cacaaacatt ggtttcaact cattaggcgg accgggaaga aggatatacg
    35521 catgtgtatc tgtttgataa aaagtcccta aagcaagccc tgtacgattt ggtagaggaa
    35581 ccccgccttc gataatctgg gactgttgca gattgttttt ggtcattttt cgatgggttg
    35641 tttcaaaata tgccaataat tttttgtatc cttccgtatt ttgtatgaga gacttaccga
    35701 ggtgagccgc agttgcttct tttgtcaggt catcttctgt cggtcctaat ccgccacata
    35761 agagtatgag ttcacttctt gtttcagcta gagcaatcaa ctcttctaaa cgctgctgat
    35821 tatctcctac aactgtttga taataaactt cgatacctag atcagctagc tgttctgaca
    35881 agaaagtggc attcgtattc acgacttgtc ctagtaaaag ttctgttccg acagctatta
    35941 tttctgcttt cattacttcc cgctccctta tatagtatac gctgtatttt tatatttttt
    36001 tatttaaaaa gagggcaagg agctgtgaca aaaatcttgt cacagctcct tgttccaaat
    36061 aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca atatacataa aacatgaaaa
    36121 gctgtttttt gaaattatcc cttgtgactc agagttagac ggcaggctca caacctctcc
    36181 actaaacgat acttacattg atcctttgaa cacaccgctg tttttgatga agtaatccac
    36241 acctgaatag atagtaaaga tcagacaagc ataaagcatg atttgatcta ctggaatacc
    36301 gatcaggctg aatgggatat tattgataaa cagcaagata atagcaacca tttgtgtagc
    36361 cgtcttgact tttcccggcc aagctgcagc catgacttct ccatgttcga caagtagtaa
    36421 acgcaagcct gtgacggcta attcacgaca gacaatgatc gctactaccc aaccaggggc
    36481 ctttccttgg tcaaccaaca tgataaaagc agtcataact agcattttat ctgccaacgg
    36541 gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca cgggcaattt ttccatctaa
    36601 ccagtctgtg atactagcta aagcaaaaat aactgcacct actagctgtg tgacagccag
    36661 tgtggtgtct cctacgttaa attgtcccca atctaaaggt aaacctacaa ccaaaatgaa
    36721 aatcgggatc atacaaattc taagtacagt taatttattt ggtaaattca cactatcgac
    36781 tcctttcttc cctattctac gtgactatca gcgaagtttc caccgatatt atttactttt
    36841 ccaaatgaat ttattcaaag gagtattttt attgaaattg atctttttga attaacgaag
    36901 aaggaccgcg acacagattt gtcacgatcc ttctttttta tacctacaag taaacagatg
    36961 cgtaaactaa tttatacgat cattctgcgt attgaagagt aaggttcaca ttttttggac
    37021 tctgattgtt ttcccctgga ttaactggta cggattctcc attcgctttg atcgttgcat
    37081 tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt ttccggtaac acggtactct
    37141 gtgtttcatt tgctgccagt gtcccttgat aaacataggc attgtctact agcacaccta
    37201 accatacacg attgacagca gtaaattcaa gggttacagg cttggtagcg tctgtgatat
    37261 ttactgcgat ggcactgcca gaatcttctc ctgtagctat tgccattttc ttttcttcac
    37321 ttgattttgt tgttgattct actgtagaag actctttggt ctgcgtgctt tgctctgagg
    37381 aagaggactc ctcgactgat ccttctactt taagcgaaga agctgtgtct ccgattattg
    37441 gatcagcttg acgatcttgc caagtcatat agcccacgac aacgacaatc gccaaagcaa
    37501 ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg acttggatgc gattcttcct
    37561 gatgcattgc ttttctagaa gtacttactg tttcaggctg cggacgttca gggaccgatc
    37621 ctgtcaattt gcttttccca tcaaaaacat cgactaaatg gtctccatct tctcctacag
    37681 cttcagcgta ttgccgaata aatgcccgca cgtaaaattt accaggcaat tggtcaaaat
    37741 catcattgtc gattgcttcc aaataacgtt tttgaatttt tgtaatctgc tgtaattcat
    37801 ctaaagatat ttttttattc aggcgtgctt gccgtaattt ttctccgatg gtttcaaaag
    37861 ccactcgttc atcttctcca gtctgtttga ttctccgaag catctgctgt tgcaacgaaa
    37921 gacgttatgt ttgggtgctg tgacaaaagt catgtcacag ctcctttttt ccgaataaac
    37981 attgttctcc tgccaactcc tcggtcacaa gtcacaatat ccaataaata taagaagctg
    38041 ttttttgaac ttgttccttg tgactcggag ttaaacggca ggctcacgac ctctttctta
    38101 aacggtgttc aatcagctgt tcctcggtat tagctcaaaa accggcaaaa tataaggaga
    38161 tatttttccg tatgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttcct
    38221 taaacggtgt tcaaaagctt gcatctgcgg taataagtcc aaccaagtta aaaacattaa
    38281 gaagctgttt ttacttggct gttcttatta cttgatgcag ggcagctttt tcacaacctc
    38341 ttggtacacg gtgttccaga agtagcttct tcggcaataa gccgaaatat cacaaaaatt
    38401 tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac
    38461 ctctttgatt acttcgtttg attttatttt aatataagtt gattaaaaga gagcatgatt
    38521 tgttttattt tctcactaat ttttccaaca caactaagaa taccataaaa gctgattttg
    38581 gaggaggttc ctactacaag tctaaaacga aatttaagac ttttcctcat aaaatactgg
    38641 tttattttgg tctcatgtaa aactcgctga aggcctcttc acttataaat gcttccccag
    38701 ctgccagtac gtctttcatt tggatcgaat cgatgatttc aggaagatca aataaagttc
    38761 gatcgccaaa taaactttgc gtaaattgat tggcaatata ttcgatcgaa tttaatgatt
    38821 ggaaatattg tccaagcatt ttcttcttca gcaaatcgag atttgtttct gacacttccg
    38881 gatcatcagc gaatccaaga atgatttctt tgactttttc tgctgctttc tctggttcgt
    38941 ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc caagctgaat tcaaaaccaa
    39001 agctgtcatc gataattcct tgattgtaca tcgctagata gtttcgagaa gtattgccta
    39061 aaatcatttg gaacaacaga ttgattgctg ttttatagcg taacagttct gttccttctt
    39121 gcggcaatgt gtctaatcct ttaataccaa gtacaaattt atctcgtgta atggctgctt
    39181 ccaatgcact ttgtttgatg atttcttttg tgttttcagg gaaataacgt acaatttctt
    39241 gctttggagg gaaatttttt gcttcttggt tttcccgaat aagtttcatc aatttctcag
    39301 gctccatctt accaactaca aataaaacca tattgcttgg ctgataaaac gtccgatagc
    39361 acgtatacaa gtcttgagct gtgattttgt cgatactttc aaccgtgccc gcaatatcga
    39421 tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc aaaaaacatc cgccagttag
    39481 ggtcgtcttc atacatttgg atttcctgac cgatgattcc tttttccttg tttacagttt
    39541 cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt cgtcagattc ttttctacct
    39601 gatccgtcgt agaaaacaga tagctggttt tcgtaaaact tgtgaaagca ttggcggaag
    39661 ctccttgttt cccaaataat tgaaagacat caccatcttc tttttcaaac aacttatgtt
    39721 ctaaaaaatg ggcaattcca tctggaactt tcaccttttc tttttcacca tagggaataa
    39781 attcgttatc gatcgaacca taattcgtac tgaacaaacc atatgtcttg tgatagtcat
    39841 ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac ttcatgataa agtgtttcat
    39901 tgatttgctc atattctgtt ttattcattt gcatttcctc ctgctaaaaa gaagattgcc
    39961 tgcaattcga tttgctcagc tacttgttgt atctctttga tcgttacgct ctccatacgt
    40021 ttcaaccatt cttcatcaga taattttgtt tccggcagcc agctgtccaa aaaagaagct
    40081 tcgattgccg cttgcggaga gtctagagaa agcagatatt ggtttctcag cattgctttt
    40141 gtctgagaca gttccaactc tgttatttca ccatttcgca aactttctaa ttgctcgtga
    40201 atcagatgaa gtacttgatt tcggttcttt tcatcgatac cggtttggac actcatgaat
    40261 cctcggaacg tatccacgct actagaggca taataagcta gactttcttt ctctctgaca
    40321 ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac cgttaaagac cattagcgca
    40381 aaacgttctg gttcatcgta atacacattt gtctgataag ctaggttcag ctttgcttga
    40441 acgattggct cacgtacttg tcgttccttc acgatattca cttgaggttg ggtataaaac
    40501 agatctggat aaactgtttc ccgcgactca aatggtaatt gcccaactgc ttcagctgct
    40561 ttgtccggat caacatcgcc gacaacaaaa atgtcgattt gatcatttgc catcatttct
    40621 tgatagtagg acgctagcga actagcagtg atcttgtcca aatcttcgac tacgcccaac
    40681 cctgggatct tttgttccgg gctgttttca aaataaagtt cttgcaaggc ataggaagca
    40741 aaagtttgtt tatcttcctt caaactttcc aaatatagac gcagattgtt cttttccaag
    40801 tcaaacgtca actgatcaaa ctgatgattt ttaatatttg gataaaataa aacctctttt
    40861 aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga catattcacc attcacaaaa
    40921 ctgatccctg cgtttagcca atgtaaattt cctttttttc taactgataa tccaaagctt
    40981 gcgccataaa gctctgcaag ttgcgcactt aactttgtct gatccgaata attcaaacta
    41041 tttgtttcca acatgctgct gagaagtgaa cgtttcgtga tgacttcttg ttgcagacgc
    41101 gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt ttgttggtaa gacatgaaga
    41161 ttcacgcctt ctgccaattg atacaccata tttatatgct ccttttcaca gttgattgcc
    41221 cgcttactct tttaatgggt caacctgcct tttttactta ttcctctgct tgaaatttat
    41281 aagttcaggc aattcgccgt tttttctaaa tgatgttaca ctttatagta tagcatagat
    41341 gttttttatc aaaagaaaca gacggctaaa agcgtatatt gtttttttat tttttatttt
    41401 tcgatatact aaagacaaat aaaagaaggg agggtcattc atgatcaaag aattcaaaga
    41461 gttcatcatg cgtggagatg tcttggatct tgctgtcggc gttgttattg gtagtgcgtt
    41521 tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca ccactaatag gttggtttgt
    41581 cgcaatgatc acaggtactt ctgatttaga aggagcactt tcaattcttg actggtcgcc
    41641 gacaaaaggc gtcacatttg cgtttggtaa cgttattagt gctatcatta catttctgat
    41701 cactggtttt gtcttattct tagttgtcaa agcggcaaac cgtgcgaaga atttacgctc
    41761 caaagaagag caagtggaag tggaagaaac tcctactgca gaagattatc ttagtgacat
    41821 acgcgatctt cttgccaaac aagtggcaga agacaataca catccagtag agcaaacaaa
    41881 tctttccgat acttctaaat aataaagaat ctaaaaaaaa gaaagccgat tttcagtgtc
    41941 ggctttcttt ttttagattc ttaacttgct acttgttcct ttttcatgtc gatatgtgga
    42001 atatcatctt ccaaatacac atctgagatt gccttgaatc caaacgactc ataaaaagct
    42061 tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg gaaaattttt ctggatcatg
    42121 ttaatcgttt cttgaacaat ctttcttcct aaaccatctt tacggttgtc ctgatgaaca
    42181 aggacacgtc caaaatggat actctccgct tcttcatata ctcgcgtgta ggcaaggatc
    42241 ttcccatcag tatcttgaaa aaacgtatgc caagcttctc catcaatgtc atcaacttct
    42301 tgataaggac actgttgttc cacaacaaaa actgcgattc gttctttgac gatcgcgtaa
    42361 aactctgctg ttgacaattc ttcaaatttc tttacctgat attccactaa gcaaacgccc
    42421 cctgcttttt tattcttagc ataccgattc gcacacaaaa aagcaaacga accttacgaa
    42481 tctcaggctc gtttgctttt taaactgctt cagttaaaaa ttcgatctca actgtccgag
    42541 taagtacatc tgaatagctg taagatactc gttcaaaaga attttcttca ggatccaaat
    42601 caacaacgaa tactgaagga taggtttctg ttaaaacgcc gcggcgttct gtttgacgct
    42661 ttcttcctgt ctgagcaacc aacatgattg ggctgccgat acgaccttct aaatctttct
    42721 tgattgaagc taaagttgtt ggcatacagt tcacctctac tatcaactat accacatatt
    42781 tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc aataaaaata aataagaagc
    42841 tcattaagat ttttctaaat tattcctcat tataaaattt caagtgtttt tttattttga
    42901 gttttactct cccgtaagcg ctcattactt gcttgtgatc cattgctaaa cattcagcca
    42961 tgtctacaaa atcaacgcct tgcatataat aatgaaaaat tttagcttct atttttgaaa
    43021 actggatttc gcaattttcg attgcttctt ttaataaaag atgctgaatt gctaaaggtt
    43081 cttgcaatgt gatctctgct aaaaagtctg gtccttctgt ctctattttt tggtcgattg
    43141 agacttcatc tttttgatca cgacgcttca gcgcgttctg cttcctcaac agagaataga
    43201 tttgattgct tataacgctt ttataataac ttccaaagct aacacctttg cttttttcgt
    43261 aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg ttcccaatcc tcagaaacat
    43321 aatttcttaa ataataggtg tttttcaaac gtagtaaaag tggaaaatat ttttgataca
    43381 gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg caataactcc tcagacataa
    43441 aataattcct ccataaattc gtttatggag gaatcttatc atcaatgatt catcgaactg
    43501 attagaacga tatgttattt attttttctc attaaatcgt ccaatttttg ggacaatttt
    43561 tttaactgct cttcattcca tggagaatta cgtcggaaat caataaaatg catatcttta
    43621 ttttcttggg ctattgtttt ttccgttttt tttatcgtct tatataattc ataagcagaa
    43681 gttcgtaatg ctccttggga aaagatcatc cactgttctg ctaaatcgct tgtcgccact
    43741 gtcacttgtg tcaaacggtc attcagctga ccagcgattc tctcgatata actgtcagcc
    43801 gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt tcttataatt ttgttggatg
    43861 cccggaacta gttgggcatc gaagacaacg atgacttcca ttccttcata tttcgcataa
    43921 tttgataaac gatgtaaaag agcttctctt gcatccgcca ttttgtcctg ctttttaagc
    43981 tggacaagtt ccggccacga accaatcatg ttgtagccgt ctacaatcaa cagctgtttt
    44041 ttcatggtct actccccctg acgttgggaa aaagcctgat acataagcaa accagcagca
    44101 acactggcat ttaagctctg gacgtgcccg gtcatcggga tcgtcagcaa ttcatccatt
    44161 tcttttttca atcctggact cattcctttg ccttcattac caatgactaa agcaattgat
    44221 cctttggcat tccatttttg gaagtttgtg ccggtcatat ctgtgccaaa tacccaaaat
    44281 cctgtttctt tgagctgttg cacagcttgt accaaattcg tcacgcgtgc aacagggaca
    44341 tgttcgactg ccccagtgga agtctttgtt acgataggtg tgattccaac tgctcggtgt
    44401 tttggtatga tcacaccatc gacgccactt gcgtctgctg tccgtaaaat cgacccaaaa
    44461 ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg ggtcttcttt tttctctttt
    44521 gttcgttcaa tcagttggtc aagggataaa tattcataag gtgtaatcgc aagaacgact
    44581 ccttgatgca cgccgtgatc gctcaaagtt tctaattttt gtttaggaac ccatttgact
    44641 ggcacagctt gttcacgtgc gatttctttc agttcttcta ctttttctcc tcgtgaatct
    44701 tcttgaagaa agagtttatt ccctcgccct tgttgtagcg cttctactgc ggcatggtgc
    44761 ccaaagacga aattatcttc taatgcttct tcattttgat cagaaactga ctgatttcgt
    44821 ccagtttgct ttttatttga atatctattt tgttttttgt cttcaaatcc acgtttcgat
    44881 tgatgtttgt ttgttctttc tttacccatt tttttcacca actttctgaa tacaccagcg
    44941 gatcaattct tccatccgct cggtttgttt tgtcaaatgc agatagccca tgagcgcttc
    45001 gaaccctgtt gcgacccgat acgtcgtaac atcagcattt tttgcgctcg tatggctttt
    45061 agcatttctt cctctgcgat aataaagttc ttcttcttct gtcaaaagat gttcttccag
    45121 catttgctga atcaaaaagg cttgtgcatt cgcagaaaca taattcgttg ccgcacgatg
    45181 cagcttattg ggttttgttt ctccttgatc aatcaggtgg tcacgaacat agatttcgta
    45241 aatcgcatct ccaacataag ccaaggctaa accgttcaat tgtgtgtaat ctctcatgct
    45301 tctcttctcc atcgggttcc ttgcggtgta tcttctaata agatcccttt ttctttcaac
    45361 aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc ttcttgcttg atttcgttct
    45421 tcgatcaatt gttcaatctc ttcatctaat aactcttctg ctgtaaataa aataccgaag
    45481 atcgaaagcc attgcgagaa taaagcttct ccttgtgcca agacagcggc agaaactttc
    45541 ggctggtcta gataagtgtt cagccactta gcaagctcat agaccactgt gatcccattt
    45601 gctgcattaa aatcatcgtc catttcttcg atgaatcgct gttctaaatt ttctaacaaa
    45661 gcgattttat ccgcatcatc tgccaaattc tctgaggcag tttcttggcg gaatttcaaa
    45721 ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg cttctttcat cgtcgtttca
    45781 ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga agaaacgcaa gacttgtgga
    45841 tcaacgtttt tgatcatttc atgtacagtg atgaaattcc ctagagattt actcattttt
    45901 tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc agtaattagc gaattttttc
    45961 cctgttttcg cttcactttg tgcgatttca ttttcatgat gaggaaattc taaatcttgc
    46021 ccgccaccat ggatatcgat cgtttctcct aaatgttttg ttgccataac ggaacattca
    46081 atatgccaac cgggacgtcc tttgccccat ggtgaatccc aagaaatctc gccaggtttt
    46141 gcttgtttcc ataaagcgaa atctaatggg tcttcttttt gctgctgttc ttcccctgta
    46201 cgctgacttg ctccgacttc cagttcatcg atggattggt cacttaattt cccataattt
    46261 tcaaatttac gggtacggta atacacatca cctgaagatt cataagcata gcctttttca
    46321 accaacactt gaataaatgc taaaatatct ggcatatgat ccatgactct tggatgcagt
    46381 gtagcaggtt cgacattcaa cgctttcgta tcttcttcaa atgctcggat gaaacgatca
    46441 gcaacttctg gtgctgtaat acctaattcg tttgctgtac ggatgatttt atcgtctaca
    46501 tccgtgaaat tcgatacgaa atcgacttta tagcctcgat attcaaaata gcgtcgaatt
    46561 gtatcgaaag caatggtgct acgagcattt ccgatatgga tataattata cacagtcggt
    46621 ccgcagacat acataccgac atgtcccttt tgtataggga tgaattcttc tttttttctg
    46681 gtcaatgtat tgtagatttt tatcattctg atctcctcac tttttctcct ttgattcgaa
    46741 cgacttttgc tggtactcct accgctgtag cgtcgtctgg aacatctttc aacacaacgg
    46801 cacctgcacc gattttggcg ttttttccga tcgttaccgg tcctaatatc tgcgcattcg
    46861 cagagagcat ggctccttgt ttaacagttg ggtgtctttt acccgtttct tttcctgtgc
    46921 cgcctaatgt tacgccatgg aataaaatca catcgtcctc gatttctgcg gtttcgccaa
    46981 tgacgattcc cattccatgg tcgatgaaca cgccagttcc aatcgttgct cctggatgga
    47041 tctcaacacc agttaggaaa cgccagaatt gagcattgat cttcgccaat aaaaagagcc
    47101 ggtgctgata taaaaaatga gagaaacgat gccaaaacaa ggcatgaaaa ccagggtatg
    47161 tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt tctctttgct gcatcaatcg
    47221 ctcgggtaaa ccaactcatg actttccact cctttcgtaa gaattttatg taggaaatca
    47281 tcttcttata aagaaaagaa gcgcctttta aataagtttc cttatcaaaa gacgctttcc
    47341 gcgtggttcc acttttcttc ataatcaatg attatctctg acagctaacg cctgttatac
    47401 gttcttggct actttgtttc ctataaaaca gtttttcctg ttttctgtgt ttcgccaaga
    47461 ccactcccag aggcatttcg gcaaccattc attgatacct tgcaccaccc ggtatctctc
    47521 ttgtaatgat cagctgctta cttttttctg ttcatcgtgt ttagttcagt acttgattta
    47581 aatgtgctaa tgatttttca cgaccaagca attcgatcgt ttcaggcaat tctggaccat
    47641 gcatttgtcc tgatacagct acacggattg gcatgaaaag attttttcct tttacgccag
    47701 tttctttttg aacagctttg atggcagctt tgacagttgg agcatctact acttccattt
    47761 gttctagctg tgctttgaat gcgtggagaa cagtaggaac tgtttcacca gcaagtactt
    47821 cttttgctgc ttcattcaat acaggatgtt cattgaaaaa tagcgtagac agatcaacga
    47881 tttctgcagc atagctcatt tgaggttgat acaggctaac gatcttcttc acccattcga
    47941 ttgtcttttc atctgggttt tctgctacac gtccatcagc gattagataa ggcagacaca
    48001 tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg gttgtttacc cattgcagtt
    48061 ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt tggatcgaac attttgatca
    48121 attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga ccagcccaat aatgcaataa
    48181 agttgaacat tgcatctggt agatagccta attcgcgata ttgttcgata aattgcaaga
    48241 ttgattcgtc acgtttgctt agttttttac ctgtttcact gttgatgatc aatgtcatat
    48301 gtccgaaggt tggtggtgtc cagccaaatg cttcatagat catcagttgt tttggtgtat
    48361 tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt catcaaatga tcatcgaccg
    48421 caacagcaaa attataagtt ggcataccat cgcgtttttg gatgacaaag tcgccaccga
    48481 tattatcgga ttcgaacgaa atagcgcctt tcaccatatc tgtaaatgcg tattctgtat
    48541 tgcgaggcac acggaaacgt acaacagatt ctagaccttg tgcttctttt tctgcttgtt
    48601 cttcaggtgt caagttcgca catgttcctg cgtaatgagg catttctcct cttgcgcgtt
    48661 gtgcttcccg ttctgcttct aattcttctt ctgtgcaata gcatttgtat gctcgattgc
    48721 tagcaagcag ctgatcaatc agcggttgat agatttcttt acgttcagat tgacggtaag
    48781 ggccgtattc tccaggattt tcaggagatt catcccaatt cattcccaac caagcaaggt
    48841 tttccaactg gcttttttcg ccatcttcga tattacgttt ttggtcagta tcctcaatac
    48901 gaataataaa gtctccatca ttgtggcggg caaacaaata attgaataat gcagttcttg
    48961 catttccaat atgcaggtgc ccagttggac ttggtgcata gcgtacgcgt actttcgtca
    49021 ttttcttcct cttctttcaa actagacttt gactaacagc aaaaattccg tcagttacgg
    49081 cttctcaaat atcatttatt taaagccact cagcacaaat tttacctttt ctaaggtaaa
    49141 tagtaaagcc aactggttga atttccttac cagttggctg aatttctttt ttattttgat
    49201 tgtttagtct ttttctctgt ctctttttta ttgtcgattc ctcgtcctgc atgtgcaggt
    49261 ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac ttgttacaac gacttcgata
    49321 cgttcattca tgtaatgctg tccatcttct acgacaacca tcgtgccgtc atcaaggtag
    49381 gctactcctt gttggcgttc tgtcccggct tttactacta gaacattcat cgtttcacca
    49441 gggatgacga ctggttttac ggcatttgcc aacgcattga tattaagaac aggtacattt
    49501 tggaattcag agactttatt caagttatag tcattggtta caatgacacc atctaaaagc
    49561 ttagccaatt tgatcaattt gctgtctact tcactgatat cttcaaagtc tccatcgtac
    49621 atttcgacag atataccctc ttctttttgc aaggcattta ggatatctaa tccgcgacga
    49681 ccacgcacac gtttcaaact atcccctgag tccgcgatgt attgtagttc gtaaagaaca
    49741 aaattaggga tcatcaaggt tccttctaag aatccagtct ttgtaatatc ataaatccga
    49801 ccgtcaatga tcacgcttgt atccaagatt ttatatttat ggaaattttc tcctgatttt
    49861 cgttctaaca gttgactctc aacttgacta gctgtttctt gctcgatttt tttgcttcgt
    49921 gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc ccattctcaa tcccagatag
    49981 cctaagatga tcatcagtaa aatcggcaat acactattga cgaatggaat ttccatattg
    50041 taaagagggg tcgagatcac aacacctaaa gtcaatccga tgatcgcacc gatcgcaccg
    50101 aagagaagat aagtcaagct gacctcactt agtttttgtt ctacttgttt gataatagct
    50161 gagatatgtt tggccaacag caaagataat aaaaagaaaa taagtgcgcc taacaggctg
    50221 ttggtaaatg tattattcag ccatccgttt gactgttgac cgattgcctg ccaagctgag
    50281 ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca tcagcaaagt aatgacacgt
    50341 ttttgcatcg tttttcctcc gtttcgacaa tcatttatta gttagcggaa cacacgcttc
    50401 aacgtttcag ccaaagttgc tactccaact atctcgattc cttttggcgc ttcccagtta
    50461 cctaagttgt ttttaggtag gtatactttt gtgaagccga gtttttggac ttctttgact
    50521 cgttgttcaa tatgactgac acgtctgatt tctccagtca gtccaatttc accgatgaaa
    50581 cattccgttg gctgtgttcc tttttcttta tagcttgaag caatactgac agcgatagct
    50641 aaatcaatcg ctggttcatt taattttacc ccacctgcag cttttaagta agcatcttga
    50701 ttctgaagaa gcaacccagc tcttttttcc agcactgcca tgatcagaga tacccgattg
    50761 aaatcaagac ctgttgttgt tcgtttggca tttccgaaca tagtaggtgt gactaatgct
    50821 tggatctcta ccaaaattgg acgtgtccct tccattgcca caacgatagc agagccagtt
    50881 gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat tagcgacttc ttccaatcca
    50941 tgctcatgca tttcaaaaat cccaatctca ttcgttgagc caaatcggtt ttttactgct
    51001 cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat ataacaccgt atccaccata
    51061 tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg taacatgacc gacgataaaa
    51121 atggctatcc catttgtttt agcggttttg agtagttctg cagtggtttc tcttacttgg
    51181 ctgacacttc ctgccacact tgtgatgtct ggctgcgtca tcgtttggat ggaatcaatg
    51241 ataacataat caggggagat atgttcgatg gcacgactga tttcgttcat atctgtttca
    51301 gcgtatagat aaaattctgt gttgatactt ccgaggcgtt ccgcgcgtaa ttttatctgc
    51361 tctgcgcttt cttcgccgga tacatataag acttttccgc ctatggctgc taattgctga
    51421 gaaacttgta acagcaaagt ggatttcccg atacctggat cgccaccaat caagaccata
    51481 gaccctggca ccacacctcc gcctaacaca cggttcagtt cttctaattt tgttttgatc
    51541 cgaggttctt ttttagggac gacgtctgcg atttttgttg gttttgcttt ttctcctgtc
    51601 aaacttgtcc gagttctgcg gtccgttgta tcttgttcga tttcttcgac catcgtattc
    51661 cattttccgc aatttgggca gcgtcccaag aacttaggcg acacataccc gcaattttgg
    51721 caaacaaatt gtacttttga tttttttgcc attgtaccgc ctccttttat cctaatcatg
    51781 agacattaaa tcaagcttaa gtatctttaa cgttttattt actggaagaa ccaaatccgc
    51841 cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa aaacggcaaa aagatccctt
    51901 ggccgatacg ctcgcctttt ttgatcacta catccttgaa accaaagttg gtgaattgga
    51961 acatgatatg gccttcattt gcctcattat tgtaataatc actgtcaatc acacctactc
    52021 cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga acggttagca agctgcaaaa
    52081 attcatcttc tcccatgtaa gctttgattc cagtaggcac gagggttggt ttcaattgtt
    52141 tttcattttc tgatacatct tttttcaatc catctgcgac tgtttccaac ccctttttcc
    52201 agcctgcttt ccagatactt ggaatcacga cctcttctgc tgcttcaaaa tcatagccag
    52261 cagcatgatg tgtagcgcgc tgcggtagat tgatattttg atctttatat ttagaaataa
    52321 cttcaaatcc tctttttctc accggttt
//
LOCUS       DAYYGS010000008        51833 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000008 DAYYGS010000000
VERSION     DAYYGS010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.008"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            163..2697
                     /gene="secA"
                     /locus_tag="ACHA44_000456"
     CDS             163..2697
                     /gene="secA"
                     /locus_tag="ACHA44_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFM8903777.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRIYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            2824..3925
                     /gene="prfB"
                     /locus_tag="ACHA44_000457"
     CDS             join(2824..2895,2897..3925)
                     /gene="prfB"
                     /locus_tag="ACHA44_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFM8903778.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            4030..4716
                     /gene="ftsE"
                     /locus_tag="ACHA44_000458"
     CDS             4030..4716
                     /gene="ftsE"
                     /locus_tag="ACHA44_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFM8903779.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            4709..5593
                     /gene="ftsX"
                     /locus_tag="ACHA44_000459"
     CDS             4709..5593
                     /gene="ftsX"
                     /locus_tag="ACHA44_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFM8903780.1"
                     /translation="MIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFMAVI
                     MNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELKKIQ
                     NEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSDRIF
                     QIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRWPFF
                     LEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMVGIG
                     VIIGSLGSIISMRRFLKF"
     gene            5745..7193
                     /gene="cls"
                     /locus_tag="ACHA44_000460"
     CDS             5745..7193
                     /gene="cls"
                     /locus_tag="ACHA44_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8903781.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            7530..8387
                     /locus_tag="ACHA44_000461"
     CDS             7530..8387
                     /locus_tag="ACHA44_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFM8903782.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            8502..9422
                     /gene="pstC"
                     /locus_tag="ACHA44_000462"
     CDS             8502..9422
                     /gene="pstC"
                     /locus_tag="ACHA44_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HFM8903783.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            9422..10306
                     /gene="pstA"
                     /locus_tag="ACHA44_000463"
     CDS             9422..10306
                     /gene="pstA"
                     /locus_tag="ACHA44_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFM8903784.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            10321..11127
                     /gene="pstB"
                     /locus_tag="ACHA44_000464"
     CDS             10321..11127
                     /gene="pstB"
                     /locus_tag="ACHA44_000464"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8903785.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            11143..11901
                     /gene="pstB"
                     /locus_tag="ACHA44_000465"
     CDS             11143..11901
                     /gene="pstB"
                     /locus_tag="ACHA44_000465"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFM8903786.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            11914..12591
                     /gene="phoU"
                     /locus_tag="ACHA44_000466"
     CDS             11914..12591
                     /gene="phoU"
                     /locus_tag="ACHA44_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFM8903787.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            13155..13847
                     /locus_tag="ACHA44_000467"
     CDS             13155..13847
                     /locus_tag="ACHA44_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8903788.1"
                     /translation="MLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAIL
                     FVVIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYL
                     TRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVI
                     GSMANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIA
                     VLFFLAVRRLKK"
     gene            13844..14611
                     /locus_tag="ACHA44_000468"
     CDS             13844..14611
                     /locus_tag="ACHA44_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HFM8903789.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            14711..16279
                     /gene="liaX"
                     /locus_tag="ACHA44_000469"
     CDS             14711..16279
                     /gene="liaX"
                     /locus_tag="ACHA44_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HFM8903790.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENSEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            16455..16763
                     /locus_tag="ACHA44_000470"
     CDS             16455..16763
                     /locus_tag="ACHA44_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HFM8903791.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            16763..17113
                     /locus_tag="ACHA44_000471"
     CDS             16763..17113
                     /locus_tag="ACHA44_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFM8903792.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            17279..18217
                     /gene="hprK"
                     /locus_tag="ACHA44_000472"
     CDS             17279..18217
                     /gene="hprK"
                     /locus_tag="ACHA44_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFM8903793.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            18232..19062
                     /gene="lgt"
                     /locus_tag="ACHA44_000473"
     CDS             18232..19062
                     /gene="lgt"
                     /locus_tag="ACHA44_000473"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFM8903794.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            19106..20131
                     /locus_tag="ACHA44_000474"
     CDS             19106..20131
                     /locus_tag="ACHA44_000474"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8903795.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            20146..21084
                     /gene="galU"
                     /locus_tag="ACHA44_000475"
     CDS             20146..21084
                     /gene="galU"
                     /locus_tag="ACHA44_000475"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8903796.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            21209..21658
                     /locus_tag="ACHA44_000476"
     CDS             21209..21658
                     /locus_tag="ACHA44_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HFM8903797.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            21651..22253
                     /locus_tag="ACHA44_000477"
     CDS             21651..22253
                     /locus_tag="ACHA44_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8903798.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(22316..23419)
                     /locus_tag="ACHA44_000478"
     CDS             complement(22316..23419)
                     /locus_tag="ACHA44_000478"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFM8903799.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            23629..24630
                     /gene="ccpA"
                     /locus_tag="ACHA44_000479"
     CDS             23629..24630
                     /gene="ccpA"
                     /locus_tag="ACHA44_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HFM8903800.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            24984..25379
                     /locus_tag="ACHA44_000480"
     CDS             24984..25379
                     /locus_tag="ACHA44_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903801.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            25376..26632
                     /locus_tag="ACHA44_000481"
     CDS             25376..26632
                     /locus_tag="ACHA44_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HFM8903802.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            26644..26967
                     /locus_tag="ACHA44_000482"
     CDS             26644..26967
                     /locus_tag="ACHA44_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903803.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            26960..27226
                     /locus_tag="ACHA44_000483"
     CDS             26960..27226
                     /locus_tag="ACHA44_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903804.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(27389..29734)
                     /locus_tag="ACHA44_000484"
     CDS             complement(27389..29734)
                     /locus_tag="ACHA44_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HFM8903805.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            30013..30915
                     /locus_tag="ACHA44_000485"
     CDS             30013..30915
                     /locus_tag="ACHA44_000485"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFM8903806.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            31126..32901
                     /locus_tag="ACHA44_000486"
     CDS             31126..32901
                     /locus_tag="ACHA44_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HFM8903807.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(33210..34796)
                     /locus_tag="ACHA44_000487"
     CDS             complement(33210..34796)
                     /locus_tag="ACHA44_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFM8903808.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            35047..35271
                     /locus_tag="ACHA44_000488"
     CDS             35047..35271
                     /locus_tag="ACHA44_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HFM8903809.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            35413..37164
                     /locus_tag="ACHA44_000489"
     CDS             35413..37164
                     /locus_tag="ACHA44_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFM8903810.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            37164..38948
                     /locus_tag="ACHA44_000490"
     CDS             37164..38948
                     /locus_tag="ACHA44_000490"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903811.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            39283..40134
                     /gene="licT"
                     /locus_tag="ACHA44_000491"
     CDS             39283..40134
                     /gene="licT"
                     /locus_tag="ACHA44_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HFM8903812.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            40231..42117
                     /locus_tag="ACHA44_000492"
     CDS             40231..42117
                     /locus_tag="ACHA44_000492"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8903813.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(42158..43060)
                     /locus_tag="ACHA44_000493"
     CDS             complement(42158..43060)
                     /locus_tag="ACHA44_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8903814.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            43290..44693
                     /locus_tag="ACHA44_000494"
     CDS             43290..44693
                     /locus_tag="ACHA44_000494"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8903815.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            44693..46126
                     /locus_tag="ACHA44_000495"
     CDS             44693..46126
                     /locus_tag="ACHA44_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFM8903816.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            46271..47197
                     /gene="trxB"
                     /locus_tag="ACHA44_000496"
     CDS             46271..47197
                     /gene="trxB"
                     /locus_tag="ACHA44_000496"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFM8903817.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            47411..49138
                     /locus_tag="ACHA44_000497"
     CDS             47411..49138
                     /locus_tag="ACHA44_000497"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8903818.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            49154..49504
                     /locus_tag="ACHA44_000498"
     CDS             49154..49504
                     /locus_tag="ACHA44_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFM8903819.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(49598..50458)
                     /locus_tag="ACHA44_000499"
     CDS             complement(49598..50458)
                     /locus_tag="ACHA44_000499"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFM8903820.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            50550..50978
                     /locus_tag="ACHA44_000500"
     CDS             50550..50978
                     /locus_tag="ACHA44_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8903821.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            51140..51316
                     /gene="rpsU"
                     /locus_tag="ACHA44_000501"
     CDS             51140..51316
                     /gene="rpsU"
                     /locus_tag="ACHA44_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFM8903822.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            51343..51789
                     /locus_tag="ACHA44_000502"
     CDS             51343..51789
                     /locus_tag="ACHA44_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFM8903823.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
BASE COUNT        16913 a         9166 c        11157 g        14597 t
ORIGIN      
        1 tttttgagtt tcctcctctt ttagatgaaa tatgaataaa ttatgaagtt cttactttaa
       61 tacgcttaag atatttcaat tatctataac taatgataaa atgaaaagta aagtctacat
      121 agtctggata atacacagaa ggaaaaggag attacacaac gcatggccaa tttgattcgt
      181 tcacttatcg aaaacgataa gaaagaacta aaacgtttag atagcattgc aaaaaaagtt
      241 gaagcatacg ctgaccaaat ggcagcatta actgatgaac aattacaggc aaaaactgct
      301 gaattcaagg gcagatacca aaaaggagaa acattagatc aattacttcc ggaagctttt
      361 gcagtcgtcc gagaagcagc aaaacgtgta ttggggttat atccatacca cgtacaatta
      421 atgggtggga tcgtgcttca cgatgggaat atccctgaaa tgcgtactgg tgaaggtaaa
      481 accttgactg caacgatgcc ggtgtatctg aatgcgttga ctggcgaagg ggtccacgta
      541 gttaccgtca atgaatattt agcaacccgt gactcgacgg aaatgggcga gctttataat
      601 ttcttaggat tgacagttgg gttgaatatc aactcaaaat cttcggatga aaaacgcgag
      661 gcttataatt gcgatatcac ttatagcacg aataatgaat taggatttga ctatttgaga
      721 gacaacatgg tcgtttaccg tcatcaaatg gtacaacgtc cgctgaatta tgccattgtc
      781 gatgaagttg actcgatctt gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa
      841 gcagaaaaat caacagcttt atatacaaga gcagacaact tcgtgaaacg tttgaaagaa
      901 gaagaagatt ataaaatcga tatacaatca aaaacaatca gtttaactga agcagggatc
      961 gaaaaagctg aagaaacatt cggtttggac aacttatatg acattgaaaa tactgcattg
     1021 acccatcact tggatcaagc attgagagcc aactacatca tgcttttaga catcgattat
     1081 gttgttcaag atggaaaagt attgattgtt gaccaattta cgggtcggat catggatgga
     1141 cgccgttatt cagatggtct tcatcaagcg atcgaagcta aagaaggcgt agagatcgaa
     1201 gacgaaacga aaacgatggc gacgatcact ttccagaact atttccgtat atacaagaaa
     1261 cttgcgggga tgactggtac agctaagaca gaagaagaag agttcagaga gatttataat
     1321 attcaagtat accagattcc gacaaaccgt ccggtcatcc gtgacgatcg accagatttg
     1381 ctttacccaa cattacaaag caagttccat gctgtcgtac aagatatcaa agatcgctac
     1441 cgcaaaggac agcctgtctt agtcggaact gttgcagttg aaacatctga gttattatca
     1501 gatatgctga atcaagaacg tattcctcat gaagtactga atgcgaaaaa tcactttaaa
     1561 gaagcagaaa tcatcatgaa tgcgggtcaa aaaggtgcag tgacgattgc taccaacatg
     1621 gctggtcggg gaacagatat caagctaggg cttggcgtaa gagaacttgg tggcctagca
     1681 gttatcggga cagaacgaca tgaatctcgt cgtatcgata accagcttcg aggacgtgca
     1741 ggacgtcaag gagaccctgg tatgtcacaa ttctacttgt ctttagaaga tgatttgatg
     1801 aaacgattcg gttcagaacg aatcaaagct ttcttagatc gaatgaagat cggtgacgaa
     1861 gatgctgtga tccaaagcaa gatgctgacg aaacaagtag aagctgcaca aaaacgcgta
     1921 gaaggaaaca actacgacac acgtaagaac gtcttgcaat acgatgacgt gatgcgtgag
     1981 caacgtgaag taatctataa acagcgtcaa gaagtgatca tggaagaaaa aagcttatct
     2041 aaaccactga tgaatatggt caaacggaca atcagtcgtg tggtagatgc gcatacgcaa
     2101 ttagaaaaag aaaattggaa tttggaaagt atcgtcgatt ttgctggcag cacgcttgtc
     2161 catgaagaca gtatttccct tacagatatc gaaggaaaaa cctctcaaga aatcaaagaa
     2221 tatctaaacg aacgagcaca agaagtattc aataccaaag cagctcaatt gaacggacca
     2281 gagcaactgc ttgaatttga aaaagtcgtg atcttacgtg tcgtggactc taaatggaca
     2341 gatcatattg atgcaatgga ccaattacgt cagtcaatcg gcttgcgtgc ctatggtcaa
     2401 aacaatccat tagtcgaata ccaaacagaa ggttataaga tgttcgaaga tatggtaggt
     2461 gccattgaat atgaagtcac tcgtatcttc atgaaagctg agatccgtca aaacgtacaa
     2521 cgtgagcaag tttctcaagg aacagcttct cactcagaag atggagatgc caaagaaaac
     2581 agtaatacta gcgcgaaaaa acaaccaatc catgtgaaca aggttggtag aaatgatcct
     2641 tgtccttgtg gaagcgggaa aaaatataag aactgtcacg gaaaagatgc aaactaattt
     2701 ctgacagcag gattggtgag acaaattctt ttttaaagaa tttgtctcac cttctttctg
     2761 ttatttgacg attctgttag actagatata ataaaaaaag tatagtgaaa gaaggaaaaa
     2821 aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc
     2881 ttcaggaggt ctctttgact tagaccagct ggaagaagac attgcggaag cagaagcaaa
     2941 aatggctgaa cctggttttt gggatgatag cgaagctgct caaaaagtga tcagtgaaaa
     3001 caatgcaaat aaagaaactt atgatcaatt ccattctctt gctgaagaac tggaagaaat
     3061 cgaattgatg gtggagatgt tgcaggaaga acctgatgca gtaatgcaag aagaagcaga
     3121 agcacgtatc aatgcgctag atgaaaaaat gaatacgtat gaattatcta tgttgttaga
     3181 tggtccttat gatcgaaaca acgccatcgt cgaattgcat ccaggggctg gcggaacgga
     3241 gtcccaagac tggggcagca tgcttttacg catgtataca cgctgggcag agcagcatgg
     3301 gtatcaagta gagacactag attatcaagc aggcgatgaa gcaggaatca agagtgtgac
     3361 cctactgatc aaaggacaca acgcatttgg ctatctgaaa tcagaaaaag gtgttcatcg
     3421 ccttgtacgt atctcgcctt ttgactcagc aaaacgtcgt catacttctt tttgttcggt
     3481 agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag atgatttgaa
     3541 gatcgacaca tatcgggcga gtggtgcagg tggtcagcac atcaataaga cagaatccgc
     3601 tgtacgaatc actcacttgc ctacaggaac agtagtagcg agtcaggcac aacgttcaca
     3661 attgaaaaac cgggaacaag cgatggggat gctgaaagct aagctttacc aattagaagt
     3721 ggaaaagaaa gaacaagaag cagctgcact gagaggagaa caaaaagaaa tcggctgggg
     3781 atcacagatc cgttcttatg ttttccatcc atattctatg gtaaaagacc atcggacaaa
     3841 cgtagagaca ggaaatgtcc aagctgttat ggacggagat cttgatttat ttattgacgg
     3901 ctatttgaaa atgcatctgc aataagagcc ctttgttaaa ttgaaataaa attgtaagat
     3961 tttgcaagca agttgaaaca ttgccatgct ataatagcta aagactgaaa aagatatgga
     4021 gagaacgcca tgattgaaat gaaggatgta atgaagaagt actcaaacgg taccactgct
     4081 atccgcaatc tctcgattgt catcgaacaa ggagagtttg tgtatgttgt tggtccgtct
     4141 ggagctggta aatctacatt catcaaattg atgtatcgag aagaaaaagc ttcaaaagga
     4201 actttgaacg tcgcaggtca tgatctaata gccatcaaaa accgcgaagt tccttatcta
     4261 cgcagagaaa tcggcgtggt gttccaagac tataaattat taccaagaaa aacagtatat
     4321 gaaaatgttg cgtacgccat gcaagttatc ggcagaaaac ctcgggatat caaaaaacga
     4381 gtaatggaag tgctggatct tgttggtttg aaacacaaag tccgagtctt tcctagcgaa
     4441 ttatctggtg gggaacaaca acgtgtttct attgcccgag caatcgtaaa tacacctaaa
     4501 gtacttatcg cggatgaacc gacagggaat ctagatccgg aaaactcatg ggaaatcatg
     4561 aaactattag atcggatcaa tgcacaagga acaactgtcg tgatggctac acataacagt
     4621 acgatcgtaa atacgattcg acaccgagta attgctatcg aaaacggccg cattattcga
     4681 gaccaagcgg aaggggaata tggatacgat gattagaacc tttttccgcc atttattaga
     4741 aagtatcaag agtttgaaac gtaacggctg gatgacagta gcttcggtca gtgcggtaac
     4801 gatcacgttg actttagttg gtgtgttcat ggcggtcatc atgaacgcaa ccaaactagc
     4861 acaagatata gaaggaaacg tggatgtatc tgtgttcgta gacattggta caaaagacga
     4921 agagatgaaa aaattagaaa aagaattaaa tgacttagat catgtcaaaa aagtcgaatt
     4981 ttcaagcaaa gaacaggaat tgaagaaaat ccaaaacgaa atgggcgatt cttggaaatt
     5041 gtttgaaggg gataataacc cgttatatga tgtatacgta gtaagtgcta ccgatccttc
     5101 ttatacaaaa caagtagcag aaaaagcggc agatttgaag aatgtttccc gttctgatta
     5161 cggtggggct tcctcagatc ggattttcca aattgctggt gcagttcgaa catggggatt
     5221 agttgcggcg gcattgctgc tgtttgtagc aatgtttctc atttctaaca cgattcggat
     5281 cacgatttta tctagacagc gtgaaattca aatcatgcgt ttagtagggg caaaaaatgg
     5341 atatatccgc tggccatttt tccttgaagg tggttggatc ggcttaatcg gtgccattct
     5401 tccgatactg atcattacat ttggttacgc atggttttac caattgatca atccgtcatt
     5461 attacgatct cattattcat tgatccatcc agaaaatatc gtttggaaaa tcaatctttt
     5521 gatggtcggt attggtgtga tcattggttc actcggctcg atcatctcta tgagacgttt
     5581 cttgaaattc tagtgtcaca agataaatat aatcagaaag ttggacgatt tcgttccagc
     5641 tttctttttt tatcagctag gtggagtaaa tggggcggct tttttctctt tactttaaaa
     5701 aacaacttgg gtattcttaa gttggtagaa aaagaggtga agccgtgaag attatcgttg
     5761 acaatttttt tacgatcctt ttgattatga atattttgct ttcctttatt atcgtttttc
     5821 gagaaagaaa ggaaactgcg caaacatggg catggctgct cgttttgatg ttcattccag
     5881 ttgtaggatt tattttatat atttttttag gaagagggat ttccaaagag aaaatctttg
     5941 atttgaagat ccaagggaaa atcggtaaga atctagaaat cgaagaagat aaacaagcat
     6001 tgatgcgagg gctttatcct catccgccta ctggacaagt agatgtgaaa caattgatct
     6061 atatgcttac tgtatttgaa agtacgcttt atacgactaa aaatgaaatc actttattta
     6121 cagatggtcg tgaaaaattc gatgcgctga tcgaggatat caaacaggca gaagaccata
     6181 tccatttcca atattatatt taccgcagtg atacattagg tgaagaagtt cgagaggcat
     6241 tgacagaggc agcaagacgc ggagtaaaag tcagagtcct tcttgatgca tggggttcca
     6301 cacaagtttc ttctagtttt tttcagaatt tgaaaaaagc aggtggagaa gtcgcatttt
     6361 tctttcctct attcgttccg tatatcaatc ctcgaatcaa ttatcgcaat catcgtaaaa
     6421 tcgtagtgat tgatgggaaa atcggatata caggtgggtt caatgttgga aacgaatatc
     6481 taggattggt aaagaaattt ggttattggc gagacaatca tctgcgtatc tatggagaag
     6541 cggtttacac cttgcaaaac cgttttttga tggactggaa ttctcagcat acaaaagaag
     6601 taggatattc accggaattt ttcccgacca ttcattctac aggtgatatg gctgttcaaa
     6661 tcgtgacaag cggaccagat accgaacatg agcaaatcaa aatgacatat ttgaaaatga
     6721 tttcgttagc caaacgggag atattgatcc aaacgccgta ttacataccg gatggatcga
     6781 ttcatgaggc attgaagttg gctcttctct caggcgtaaa agtccatatc caaataccta
     6841 acaaaccaga tcatcttctc gtttactggg caacgtattc tttcgctgca gagctgatcg
     6901 agtatggggc aagaatcgaa acatatgaaa atggatttat ccatgcaaag acgatgatta
     6961 tcgatggcgg catcgtttcc gttggttcag ctaatatcga cgttcgctca tttagattgg
     7021 attttgaagt caatacactg atctatgatg aacgaatggc ttctcgcgta agaaaagcat
     7081 tctttgaaga ttcaaagatt tcgacgcatc tcacaaaaga agtgtatgaa aaccgaggga
     7141 tgatgatcaa gatgaaagaa ggattagcaa gactgatttc gccgctcctt taaaatacaa
     7201 aaaggacagg agagccatca atagatggaa atctcgcgtc cttttttctc ggggccattt
     7261 ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag cgaaattaac tttggggtat
     7321 ctcttgtttt ttggggaaag caagaaataa gctaatatat gctattaaaa gagacacatt
     7381 tcaaagccag tatagcatag aaacttttaa tgagatagct ttttttgcaa ttatgagaat
     7441 ttattttgtt atttaatgat tttgattcgt tttcatcaag taatgaaaat gatagaataa
     7501 acatacttta ggattaaagg agatttctaa tgaaaaaact aaaattattc ttaccaataa
     7561 taggattagc tattctttta tcaggctgtt caaagtggat tgatcgagga gaatccatta
     7621 ctgcagttgg ctcttctgcc ttacagcctt tagtagaaac ggttgctgaa acctaccagg
     7681 cagaaaatcc tggaaaattc atcaatgtac aagggggcgg aagcggaact gggttgtctc
     7741 aagtacagtc aggagcagtc gatatcggaa attctgattt gttcgcggaa gaaaaatcag
     7801 ggattgatgc ttcggcatta gtcgatcatc gagtagcagt tgttggaatc actcctattg
     7861 tgaacaagga tattggcgta aaagatatca gtatggaaaa cctgaaaaaa ttatttacgg
     7921 gcgagatcaa aaactggagt gaagtcggtg ggaaagatca gccagtagtt atattgaacc
     7981 gtgcatcagg aagcgggacc cgtagtacgt ttgaaaaatg ggtattggac ggagaaacag
     8041 ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg ccaaatcgtt gcggacacac
     8101 ccggagcaat cagctatgtt gctttttctt acgtttccga tgatgtggat acgttgaacg
     8161 ttaacggtgt ggaaccaaca gatgaaaatg tgacaacgaa caaatggacg atttgggcat
     8221 atgagcatat gtacacaaga gaccatccaa aaaaactgac aaaagaattt ttagactata
     8281 tcttatctga tgaagtacaa gataatattg tcggagagct aggctatatc cctgtttcac
     8341 atatgaaagt tgaacgtgat tggcaaggaa acattgttaa agagtaatct ttacacaaaa
     8401 tttacaaact tagctaaact tttacatgtt attaaaacca aattgatgtt caatttgtac
     8461 actgagtgag tatgttaaac ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag
     8521 tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg cggaaaattc attagttttt
     8581 tgtgtatcgc gctgatcgtg ttggttgtat tatctatttt ttattttgtc gctagtaaag
     8641 gattggccac cttttttact gataagatca atgtatttga ttttctgttt ggtacagatt
     8701 ggaatccaag ccaaatagga gcaaatggga aacccatggt aggggcattg cctatgatca
     8761 caggttcatt tattgtgacc tttttatctg ctattgttgc cacaccattt gctattggag
     8821 cagcagtgtt catgacggag atttctccta aaaacggaac gaagatcttg caaccggtca
     8881 tcgagctact tgtaggtatt ccgtctgtcg tttacggatt tatcggttta tctgttatcg
     8941 tgccttttgt cagaagtatc tttggcggga cagggttcgg tatcttagct ggtacatttg
     9001 tgttattcgt tatgatctta ccgactgtca caacgatgac tgtggatgca ttaaaggcag
     9061 tgccgcgaca ctatcgggaa gcttcattag cgctaggtgc cacaagatgg caaacaatct
     9121 acaaagtggt gctgcgagca gcagtgcccg gaattttaac agcagtcgtt tttggtatgg
     9181 ctcgtgcgtt tggcgaagcc ttagctatcc aaatggttat tggtaatgcg gcattaatgc
     9241 caacaggact tactactcct gcttctacat tgacgtccat cttgacaatg ggaatcggaa
     9301 atacaatcat gggaacagtt gaaaataatg tgttatggtc attagcgctg atcttgctat
     9361 tgatgtcatt attctttaat atcgtgatcc gtatgattgg gaagaaagga gagctgaaat
     9421 aatgaacgca aaacgctcag ataaaattgc tacaggtgtt ctatatgtgg tttcagggat
     9481 catcgtattg atcttagcag ctttattact ttatatctta gttcgaggaa taccgcacat
     9541 ttcatggtca ttcttgactg aaccttccaa agcctaccaa gaaggcggtg ggatcgggat
     9601 tcaattattt aactcgattt atttgctttt gattaccatg atcatcagtt ttcctatctc
     9661 tttaggtgcg ggtatctacc tttcggaata tgccggaaaa aactggctga cagacgtgat
     9721 ccgtacgtct atcgaaatct tgagctcttt gccttctgtt gttgtcggtt tattcggttt
     9781 cttggttttc gttattcaat tccagtatgg tttctcaatc atttctggtg cattagcttt
     9841 gaccgtattc aacttgcctc ttttgactcg aacggtcgag gaatctttac aagcggtcca
     9901 ttacacacaa cgagaagctg gattggcgtt aggtttgtca cgctgggaaa cagtgataaa
     9961 agtggttgtt ccagaagcga caccaggaat tttgactggt gtgatcctaa gttcaggaag
    10021 aatttttggt gaagctgccg cattgattta tacagctggt caaagcgcgc cagcattaga
    10081 ttttacgaac tggaatccat taagtgtttc tagtccgatc agcattttcc gccaagctga
    10141 aacactggct gtccatattt ggaaaatcaa cacagaaggt acgatgcctg atggtgtttc
    10201 tgtttcggct ggcgcttctg ccgtgttgat cattgctgtt ttactgttca acttcggcgc
    10261 tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca gcctaaggag aagaagtaaa
    10321 atgaaaaaat ataattggaa tgaaacaaat gtcattacca tgccaaaaga taaggaagtt
    10381 gcattatata cgaatgatct gcatgtttgg tatggcgaca atgaagcaat caagggagtg
    10441 gatctagaat tcgaaaaaaa taagatcact gcattgattg gtccctctgg ctgcggaaaa
    10501 tccacgtatc ttcgttcttt gaatcgtatg aatgatggga ttgctaatac gaaaattact
    10561 ggaaagatta tgtataaaga cgtggatatc aatgcgcctc aagtggatgt ttatgaaatg
    10621 cgtaaacgga tcggcatggt gttccaacgc cccaaccctt ttagcaaatc gatttatgac
    10681 aatataactt ttgctttgaa acagcatggt gaaaaaaaca aaaagaaatt agatgaaatc
    10741 gttgaaacaa gcttgaaaca agcagctttg tgggatcaag taaaagatag cttggataaa
    10801 agtgccttgg ctttatctgg cggacaacag caacgtttgt gtatcgctcg tgcaatcgcg
    10861 atgaaacccg atatcttgct gttagacgag ccggcaagtg ccttggatcc tatttcgaca
    10921 ggaacggttg aagaaacact tgtaaacttg aaagaagact acacgatcat tatcgtgacg
    10981 cataatatgc aacaagcagc tcgtatcagc gactatactg cctttttcta tctaggaaaa
    11041 gtaatggaat atgacaaaac aaacaagata tttaccagac caaaaattca ggcaaccgaa
    11101 gattatgtgt ccggtcattt tggttaagac ggaggcctca acatgacaaa agaaattatc
    11161 tcatccaaag atcttcatct gtattatgga aagaaagaag cactaaaggg aattgatcta
    11221 agtttcaatc aaggtgaaat cacagcgatg atcggtcctt ctggttgcgg gaaatctacc
    11281 tatctacgtt cgttgaatcg aatgaacgat ctgattccaa atgtaacgat caccggaagt
    11341 gttttatata aagacaaaga catctatggt ccgaaaacag atgttgttga actaagaaaa
    11401 gaaatcggca tggtgttcca acagccaaat ccatttcctt tttctattta tgaaaatgtc
    11461 atctatggat tgaagttaaa aggtgaaaaa gataaaaaag tccttgatca agtagtggaa
    11521 gaaagtctga aggcagcgtc cgtttgggag gatgtgaaag ataaactgca taaaagtgcc
    11581 ttgtctttat ccggtgggca gcagcagcgt gtatgtatcg cacgtgtttt ggcagtgaat
    11641 cccgaaatca tcttattgga cgaaccaaca agtgcacttg accctgtttc tactgggaaa
    11701 atcgaaagta tgctgttaga attaagagag cggtatacga tgatcatcgt gacccataat
    11761 atgtctcaag cttcacgtat ttcagataag acggcattct ttttagatgg tcacttgatc
    11821 gaatacaacg atacgaagaa gatattcatg aatccagaaa aacaagagac agaagattat
    11881 atttcaggtc gatttggata aggaggaaac ggaatgctac gtacacaatt tgaagaagaa
    11941 ttgctgaatc tccataatca gttctacgaa atggggatga tggtgagcag cgctgtgcac
    12001 aagtcagttc gtgcatacat caaacacgat aaaatcttag ctcaagaagt tatcgaaaac
    12061 gatataaata tcaataatat ggaaacgcgt ttggagaaga aaagttttga aatgattgca
    12121 ttacaacagc cagttacaac tgacttgcgt atgattatca cggtcatgaa agctagctct
    12181 gatttagaac gtatggccga tcatgcggtg tccgttgcta aatccacaat ccgcgtaaaa
    12241 ggacagacga gaataccgga aatcgaaaaa gagatctccg atatgtccga ttacgtgaaa
    12301 aaaatggtgg acaacgtcct agttgcgtat gtcaaaacag acgaaaaaga tgcgcgtaca
    12361 atcgccaata tggatcaaag agtcaatgac tatttcaatc gaatctataa tgcaaccatc
    12421 aaaaacatgc aagaaaatcc tgaaacagtc atcagtggca cagactattt gaatgttgcc
    12481 agctatctag aacgaatcgg agactatgtg acaaacatct gtgaatggat cgtctacttg
    12541 gctacaggga aaatcactga attaaatacg aaccacaatg agattttcta aactgaagtt
    12601 gtgattctgc tgtttattta ggaaaagagg ttgtgaaatc agcgttttgt cctgagtatt
    12661 agagaaacaa acggtaaaat agttcctcat attttgccgt tttttgagct aataccgaag
    12721 gtcagctgat tgaacaccgt ttattctgga aaagggtttg tgacaaaaca tgtcacaaac
    12781 ccttttcatt attccaatag aaaggataaa gaatgcaaaa aaagattagc atcatcatca
    12841 ctctattttt tatttgtata cttgcttcat ggtttattca gcgtcaagct gctctatatg
    12901 acgtacctgt catcgagatc gaaagagtga gaatagaaga ggatcaacaa ttgattacag
    12961 gaagattgat gaatacggac caaaaaggag aagtgatttc tttaacggct ccttatcaaa
    13021 aaaatcaaat cgaaacagca aatataacaa caagacaaat ctatcttgtc caaatgcatg
    13081 gagatgaatg gcaactagta gcaaaaaaaa atgatggctg gctttttttc tttacaagtg
    13141 tcttcattgg caccatgttg cttattggtg gaaaacaggg cgtatttgct ttaggaagtc
    13201 tcatcatcaa catcgtttta cttttacttt tgttgctcct ctttaccaaa acagaaggta
    13261 tttctctctt aaatatagca atcttatttg tcgttatagg gataatcgtt agtatattgg
    13321 cgttagacgg atggaataga agcagtataa tcaaaatttc ggctgcagtt acaagtgttt
    13381 ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagatcaa ggactgcgct
    13441 atgaagaact gaattatctg acacgaccgt atcgttctgt ttttctttcc agcgtgctga
    13501 ttgggacagc tggagggaca ctcgataccg tgatcacagt cattgcgaca ttagaagaat
    13561 tgaccaagca acaaccgaaa atatcagcga aacagttatg gctttcagga aggcaagtgg
    13621 gccgagatgt gattggttcg atggctaata tccttttatt cgtctatatc agcggagcga
    13681 ttccatcgct tctgttatat ttaggaaatc aatggtcttt caaagaaacg gtagaacgac
    13741 atttatcatt agaactttta cgcgtcattg ctggcagctt aggaatagta ttatcaatcc
    13801 ccatcgcggt cctctttttt ctcgctgtaa ggaggttaaa aaaatgaatg tacttattat
    13861 acttgttggg atttttgcaa tcagtgtgct atttgtcggt ggtacgcaag gtatgtatat
    13921 tcttttagga ttattcatta atttaggaat ctttttccta ttattattcg gctatcatca
    13981 gaagtggccg atattggtat tgtcgattat agggttccta ttgatcgctg tggttatctt
    14041 attttttatc aatggttata atctgaaaat gcgagccgct tttgccagta tactgatctt
    14101 tttgttttgt ttcctactat tgattcctat cactgatttt ttagcgatcc aaggctttac
    14161 ttcgatagaa ctagaagaac tatctggctt agataagacg ttagcaattg attttagact
    14221 acttacgagg tcgttgcttc tgatcagtct tagcggagct gttttagatg cgagtgtagc
    14281 gatttctagt ggaacattcg aagtatatca ggcaaatccg caaatgtctt ttaaccaatt
    14341 gcgccatgct agctttgcca ttgctaaaaa aatattggca tcaacagtga tgacgcttct
    14401 ttttgcgttt atggggagtt ctttagcgtt gattctttgg tttattgact tagacatttc
    14461 ttttcagcaa ataatcaatg aaaaatcatt tgtcttggaa tatacgatgg ctattcttac
    14521 cacactggcc gcactacttg tcctgccctt aacttgcgtg actacagcat atctctttac
    14581 aaaaaagaaa gaacatctca agatggagta aaagttacaa cttaagtcct atgacatttt
    14641 tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt tgcttaagga
    14701 ggcaatttcc atgaaagaaa gagaacgtat tttagactta gtgaaaaaag gaatcctttc
    14761 gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg aaaaacaaat
    14821 caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg caagtcaatt
    14881 gatcgatgaa cttgaaaaca gtgaagaaaa aatttctgag ccagttgatc caaaagaaaa
    14941 agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa ctgaagcgaa
    15001 taaaacttcg gcgcgtctag acgaagtaaa tgctcaattc gctgacaata aggcagcacg
    15061 taatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag gcgagttgac
    15121 agaagaagaa ttagcacagc gtcaaacgtt agaagcagaa atcaaagagt tagaagcaac
    15181 cggcgataca ctattagaag aacaattgaa gctggaagca gaattaaaag atatccgcaa
    15241 gaatcaatgg tctgagaaaa aagaaacatt tacggataag tttgaattgc cagatgattg
    15301 gaaagatcaa gcgacagata cgctgaatca agtaggcgaa aaaatgtcag aagctggatc
    15361 acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag taaatgataa
    15421 catggaatgg aaagatgtca gcttacgcgt ccctggaatt gcgacaacga aatttgaaca
    15481 cgaattttat tatgaagctc ctgcagcaag tatccttgat atcaaggcag caaacggaaa
    15541 cgtgacgtta aaaacgtggg acagtgatga tgtgaaagtg gaagccaaga tcaaacttta
    15601 cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga tcgaggtcaa
    15661 tgaagatcat atctccttcc aaattccaaa taagcgtgtg cgtgcagacc ttgttttcta
    15721 cttgcctaaa cgagtatacg accatgcagc tatcaaactt ctaaatggca acatcatgat
    15781 tgagacattg gaagctaaag atatttatac aaaatctacg aatgggaaca tcatcgtgaa
    15841 ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa
    15901 cgggaacatc ttagattcta ttatagaaac agtaaacggt acagtgacat ttggtgccac
    15961 accagaaaac ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga ttaaagaaga
    16021 taatttgaaa aaagtagaag caagttcagt gaacgggaat gtcaaagttg ctttaccaga
    16081 tagcatcggt ttggaaggac acgctaaaac aagtcttgga agcattaaca gccgtctgtc
    16141 caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc aattccgtcg
    16201 tgtttcagat gatgagatcg cacaagtgca gctttcaacg acaacaggaa gtatctattt
    16261 aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag caataagaag
    16321 cgatttttta aaatagcttc ccctatttta aaaaaatgtg ccttattgcc gataggctat
    16381 ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt aaaatagtag
    16441 aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat gttgttttga
    16501 caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt atccgtgtgc
    16561 tttatgtatt cttaagctta gttgcttttg gaagtccaat cattttatac attttattag
    16621 ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac aatgcatatt
    16681 atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa aaaatcgatg
    16741 atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta atacgttaac
    16801 attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta tttggacggc
    16861 cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga ttttgacgat
    16921 cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta tcaatgcagc
    16981 gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt cttcatttgg
    17041 tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaattgtta gtgaacataa
    17101 cagcagaaaa taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt ttagaataaa
    17161 gaatgcgttt tctattacat tggtaattaa ataaaaacgt tttttttaat tggtagtact
    17221 tttggctaga caatgataaa atgatagaga acactctaat agaaatgagg tagcatgaat
    17281 gtcagaagtt gtaaaagtta gccaattagt aaaaaaatta tctttggaaa tcgtcacagg
    17341 cgacgagaaa agtttagacc gcgtcatcgt gactggggac atctcacgcc caggactaga
    17401 gttgaccggt tattttaatt attattccca taatcgttta cagctatttg gcagtaaaga
    17461 aatcagtttt gcggaacgga tgatcccaga agagcgtttg atcattatga gaagattatg
    17521 tagtgaagat atgcccgcat ttattatttc aagaggattg ccggctccgg aagaaatgat
    17581 ccaagcagcc aatgaaaatg ggatagctgt gctgcgttcg cctatctcaa cgtcccggct
    17641 gctaggtgaa ttatctagtt atttagatgg acgcttagcc cctcgaacaa gtgtccacgg
    17701 tgtgttagtc gatgtctacg gattaggtgt actaattcaa ggggacagtg ggatagggaa
    17761 aagtgaaacg gcgttggaac tgattaagcg cggacatcga ttgatcgcag acgatcgagt
    17821 agatgtctat cagcaagatg aactgacggt aatcggagaa cctcctaaaa tcctggaaca
    17881 tctgattgag attcgaggga tcggtatcat agatgtaatg aatcttttcg gtgcgagtgc
    17941 ggtacgaggc tctatgcaag tccagttagc tgtttatcta gaagcatggg cgaaagataa
    18001 gaaatacgac cgtctgggct ctgaagacac gaccgttgaa atcgcggaag tgggcattcc
    18061 acaaataaaa atccctgtca aaacaggacg aaacgttgca atcatcattg aagtagccgc
    18121 aatgaatttc cgcgcacgta caatgggctt tgacgcaacg aaaacatttg aagaaagatt
    18181 aagtcgcttg attgaagaaa actcaggcaa tgactagcaa ggagtgacaa tatgttagca
    18241 caaatcaatc gagttgcttt tgagcttgta ggaattccta tctactggta tgcattgatt
    18301 atcgtatcag gaatcattat tgccatgtgg ctcagcagca gagaagctgt tcgggtagga
    18361 ttaaaagcag atgacgtgac agattttatg ttgtggggat tgccgatttc cattattggt
    18421 gcccgcttgt attatgtttt attcgatctt ccccaatata tagctgatcc aatccagata
    18481 ttcaatatcc gttcaggagg attagcgatt tacgggggat tgattgcagg tgcaatcaca
    18541 ctgtactttt ttaccaaata tcattttatt tctttgtgga cctttttaga tatcgctgca
    18601 ccaagcgtat tattggcgca ggcaatcgga cgatggggaa atttcatgaa ccacgaagcg
    18661 tatggtccag acacgacacg aagtttctta gaaagtctgc atttaccgca attcatcatt
    18721 gataacatgt atatcgaagg aatctatcga caaccaacct ttctttatga atcactttgg
    18781 agtttgattg gatttattct gcttttggtt ttgcggaaga aaccacatct tttgaaaaaa
    18841 ggagaagtcc ttttatgtta tctaatgtgg tatagtttcg gacgtttctt tatcgaaggg
    18901 ttacgcacag acagccttta ctttttaggt gtgatccgca tttctcaatt attgtctgct
    18961 gttttatttg tcggcacatt gattctgttt atttggcgta gaaaaaaagg aagtttaccg
    19021 gattatgacc gctcattagg aaaaaatcaa caaattattt gattttcaga gcgttaaaaa
    19081 tgaaaaaaat ttcaggagtg gtaaaatgaa ttatagagta gcggttttag gaccaggctc
    19141 ttgggggact gctttagcac aagtcctcgc tgaaaacgga catgacgttc ggatttgggg
    19201 acatcgtgct tcacaagtag aagaaatcaa cacccaacac accaatcaac gttatttacc
    19261 agatcatcat ctacccgatt ccatccgagc attcaccgat atggaagatg cagtaaaaga
    19321 tgcagatgcg gtactattcg ttatcccgac gaaagcgata cgttctgttg caaaagaact
    19381 agttgccaaa ttgaatacga agccagtgat catccacgca agtaagggat tagaacaggg
    19441 aacgcataaa agaatctctg aaatcttgtt agaggaaatc ccgacagaaa aaagaaaagc
    19501 tgttgctgtt ctttctgggc caagccatgc ggaagaagta gcaaagcatg acattacgac
    19561 gatcacagcg gcaagcacaa atgtaaaagc agcaaaatat gtacaagagc tgttcatgaa
    19621 ccattatttt cgaatctata ccaaccctga tgtgattggt gtagagatgg gagcagcttt
    19681 gaaaaatatt attgccattg gagctggtgc tattcacggc ttagggtttg gcgataatgc
    19741 aaaagcggca atcatgacgc gcggattagc tgagatcagc cgtttaggtg tagcaatggg
    19801 tgccaacccg ctgaccttta ttggcttaag cggagtaggt gatctagtcg ttacttgcac
    19861 aagtgttcat tccagaaact ggcgcgcagg caacttgtta ggacaaggtc agcctctaga
    19921 agaggtgcta gccaatatgg gaatggttgt tgaaggtgtg aatactactc aagcagcgat
    19981 ggaactagcc caaatgatgg aaatcgaaat gcccatcact caaacaatct atgaagtgct
    20041 gtatgaacaa aaagaaatca aaacagccgc aaaagaaatc atgttacgtg atgggaaaaa
    20101 agaaaatgaa ttctcattaa caattttttg aaggagcgaa tgtatatgaa agtaaaaaaa
    20161 gcagttattc cagcagcagg attaggaaca agatttttac cagcaacaaa ggcgatggca
    20221 aaggaaatgt tgccaatcgt ggacaaacca acgatccaat tcatcgttga agaagcgttg
    20281 aaatcaggaa tcgaagacat cttgatcgtt acaggaaaag ctaaacgacc aatcgaagat
    20341 cactttgatt caaatatcga attagaaaca aacctaagtg aaaaaggaaa aaccgaatta
    20401 ttgaaattag tagaagaaac gacagatgtc aatttgcatt tcatccgtca atctcatcca
    20461 aaggggttag gacacgccgt tcttcaagca agagccttta ttggaaatga accgtttgtc
    20521 gttatgctag gcgatgatat catggaagac gaagtaccat tgacacagca attgatcgat
    20581 gactatgagc gtacacacgc ttctacgatt gccgttatgc aagttcctca cgatgaaact
    20641 tcaaaatacg gaatcatcaa cccaggggaa gtactggaag atgggttata caatgtcaaa
    20701 aactttgttg aaaaaccaga tccaagcaaa gcaccaagtg atttagctat tattgggcgt
    20761 tacttattga ctccagaaat ctttgatgta ttatcagaac aacaaccagg cgctggaaat
    20821 gagatccaac taactgacgc aatcgatacg ttgaataaaa cacaacgcgt atttgcccgt
    20881 aaattcacag gtaaacgtta tgacgttggt gacaaatttg gttttatgaa aacaagtatt
    20941 gaatatggtc tgacacatcc agaagtaggg gcacctttgc gtgaatacat catgtcttta
    21001 ggtaaaacat tagaagcaga aggaagcaag atcaatcata atccaaaagg aataacagaa
    21061 atcaaagaag aaaagaaaaa ataatctttt tttctaacga gcttcagctg ctaattgaat
    21121 aaaaagtagt tgaagctcgt ttttatttgt ttaaaaaaca cgaaaatgct atccttatag
    21181 aaaagagaga caagaaggga gtttgatcat gtcaggagga gaaattgcag gattgatcgc
    21241 agcaatcgcc tttgcgatac ttgtcgtttt catcgttcgc gtattgttgc aagtttccaa
    21301 aactttagaa aaagtagacc agactgtcgc agaagcaaat acgacaattg agatcgtcac
    21361 acaagacgta gatcttttat ctagacaagt agaaggcttg ctagtcaaaa gtaatgagct
    21421 gctcttggat atcaatggaa aagtcgcaac gatcgatcca ttgttcacag cagttgctga
    21481 tttaagtgaa agtgtatcgg aacttaatca ttctagtaaa aacatcgtga cgaaagtcgg
    21541 cggaatcgga aaaacgacag ctaaagcgac tgttgccgga aaagtcggtg gaacagcgat
    21601 gaaattttta aaaacaaaaa acatactgaa acgacaggag gaaaataaga atggctaaaa
    21661 aaggtggatt tttattaggt gcagtagttg gaggtacagc cgcagcaatc gccgcattat
    21721 tgttagcacc caaatctggt aaagaacttc gggatgaatt ggctgctcaa gcagatgatt
    21781 tgatggacat ggcttcagat tacacagata tggcaaaaga aaaatcatct gagttaacag
    21841 gtattgcaaa agaaaaagcg caagatcttt ctcaacaagc aggggatcta gcaggcaatg
    21901 ttaagcagaa ggtaagtgaa tcagcagagg aagcaggaag cgaatcagat gatatcttgt
    21961 taaacctcaa agaacaatca gctgatttat ctaatcgttt caaaaaaaca gcagaagatc
    22021 tgaaaaagcc agcagacgaa ttaggtagta ttgctaaaga tacttcagaa gatatcatga
    22081 tcgatgtaaa agatgctgca gataaagcag gagagacggt caatgaagga atgtcagaag
    22141 caaaaccagt tgcagatgac acaaaagaaa tgttgaaaat ggcaaaagaa gatgcaaaag
    22201 aagctaaaaa agaaatgaaa gatacaatga cagattcgac taccaatcac taagattact
    22261 catttctaga ataaaaaagg tgagagcgga ccagctctca ccttttttat tttcattact
    22321 ctaaaacaag taattctttt ggtgtagtcg tgaatggcac acaaccatca cttgtcacat
    22381 acacacaatc ttcgatacgt acccctactt tttcaggaat atagattcct ggttcaagag
    22441 agaagcacat cccttcttca atcactaagt cgtttccttc tacaagagaa gggaattcgt
    22501 ggacggttgt tccgattccg tgacctaaac ggtgattgaa atattcgccg tagccatagc
    22561 tgttgatcac gtttcgtgcg atttgatcaa gttcacctgc tgtcacacct gggcgaactg
    22621 cctcttgtgc ggcaagctgt gcttcaagag tgatgttata gattttttct tggaattcag
    22681 ttggtttttg ataagcaact gtacgagtag catcactgca atagccattc caaataacgc
    22741 caagatcgaa taacactagt tcatttggtg aaaccttcgt gcttcctggt gtaccatgag
    22801 ggcttgcagc atttgctcct gcaaggacta atgtgtcgaa agacatttgt gaaacacctt
    22861 gtttttttaa ttggtattcg atttctgcga taattgcttg ttcagttacg ccttctttta
    22921 ctgttttgaa gccaatttca aaggcaacat cggcccattt accagcttcg atcagtttgt
    22981 ctttttcttc taccgttttg atcagttgca gacgctggat aatagaagtt agatcggtgt
    23041 aagaatctgt acctgtcaag atatcagaga tagcatcgaa gcgatcgaca gtcagagcat
    23101 tcttttcaat acctacatta ccaggttgcc catagcgtgc gcgaagctct tttccgatga
    23161 tctcccaagg attttcgctg tctaagtagc cacgtacatc gtacgcccag ctgctgtttt
    23221 gagcatcttc aacttccaga gcaggagtga aaaggaaagg atcttgttcc agagggataa
    23281 aaagagctag tacacgctcg tgaggatcac tttcatatcc ggaaaagtaa gcgatgtggc
    23341 cagggtcgct gatataagcc aattctaatt ggtgttgtgc catccattga cgtagttcat
    23401 tgatttttac gttattcatt tggcaaccta ctttctaaat taattttgta ttcaataatt
    23461 gtaccactga ttgattaaaa gaccaacgtt cttaaaactg aaaaaagtaa aaattttcat
    23521 gaaaacagag ataaaacggt tgcaattgtt gagattattt gatatcctat tttagaaatg
    23581 taagacgtat tttcagaaaa catataaaat atgaatagga gaacacaaat ggaaaagcaa
    23641 acaatcacta tctatgatgt agcgagagag gcaaatgttt cgatggctac tgtttcacgt
    23701 gtcgtaaatg gcaatccaaa cgtcaaacca gcaacaagaa aaaaagtctt ggaagttatc
    23761 gatcgcttgg attatcgtcc aaatgcagtc gcacgtggct tggcaagtaa gaaaacaaca
    23821 acagttgggg taatcattcc tgatgtcagc aatatgttct ttgcctcttt agcacgtggt
    23881 atcgacgatg tggcaacaat gtacaaatac aatatcatct tagcaaactc agatggcaac
    23941 gatcaaaaag aagtcaatgt attgaataat ttattggcta agcaagtgga cggcgtgatc
    24001 tttatgggtc accatatcac agacgaaatc cgtggagaat tttcacgttc aaaaacgcct
    24061 gttgtactag ctggttccat tgatccagat gaacaagtag gtagcgtaaa cattgattat
    24121 gtatcagcaa caaaagatgc agtaaataaa ttagcaaaaa gcggcaataa aaaaatcgct
    24181 tttgtcagcg gtgcgctgat tgatcctatc aatggtcaaa accgtctaaa aggctataaa
    24241 gaagcattaa gcgaaaacaa tctaccatat agcgaaggat tgatttttga ggcacaatac
    24301 aactttaaag atggtctgtc tctagttgac cggatccata atagcggtgc aacagctgtc
    24361 tatgtttcag acgatgaatt agccattggt atcttggatg gtttattaga ccgcggcgta
    24421 aaagtaccag aagaattcga aatcatcaca agcaacaatt ctttactgac agaagtagct
    24481 cgtcctcgtt tgacaagtat cacacaacca ttatacgatt taggagcggt atccatgcgc
    24541 ctattgacga aaatgatgaa taaagaagaa gtcgaagaaa aaacgattat cttgccttat
    24601 ggaatcgaag aaaaaggatc aactaaataa taaaagatca atcatttaaa ggttgttccc
    24661 aaagtggtca acttctggaa caacctttat acgagaaaag gggttatgac aaaattttgt
    24721 cataacccct ttttttgtga ctttaaaaaa aagagagaat ggcggaaaca aaccttacgt
    24781 ccatcgtata cgacacaatc tgtgcgtaga tgctacattt tgttcactac tatcaaaatt
    24841 actttaaaat gaaaacgtga taaaaaagaa agtagaaaag tagaaaagta actctaaaaa
    24901 atccaaaaaa atccaggctc gattgcgtac ttatcttaaa ggagaaacaa tccatcatac
    24961 ataacccgaa aggagacttc tatttgccta agaaaagacg aaagaccata gtcatcattt
    25021 tgaccattct tactatagga atcggaggaa aattttacat ggacaaaaga gaagcgcaaa
    25081 aacagcaaga tttactcgca gtagaaaaac agtccgtcaa agttctgaaa aatacctttg
    25141 cggatatcaa agaagtgaaa atcgaacaat ttgagaaaaa cgacatgaca ggaatctatg
    25201 aagcattgat aacgatgacc aatacagaag gaaaagccgt ttattttgat tatagcttta
    25261 caaaagaaag caatgagata gatggctatg gtgttgaaaa tgaagaagta caaaaagaag
    25321 ggattacggt tgatgagatt atagttatct atacagatgg gagtgaggaa aaagtatgac
    25381 aattactgat aagcagtata aaggaataag taataaagta tattggctag atccaaaaca
    25441 tcctcaatat agtaaaagtt acaaagaagg cgccattaaa aactttggag aaacaaaatt
    25501 ccaaatccta aaaatcaaaa actccttcga cggcatgcag gcgatggctg tagcgccaat
    25561 caagaacggt aaagtggata tttctcaagt agtgattgct tttgcaggaa cgaacttaag
    25621 cgattggaaa gatttggaaa cggatgcaag aagtgttatg aatctctatg ggaatagacc
    25681 aggagcaagt ttttctgaaa aagcaacaac aaacaagatt ttatccgtta gtggattgaa
    25741 aacagcgggg caaatcaatt cagcgattgc atttgctaat gaagtaaaaa aggaatatgg
    25801 agaagctgct atttctacta ccggccattc attaggtgga ttcttagcat tatatgtagc
    25861 agcagagaaa caatggaaaa atgtcggctt caacgggccg gacccgtatg atctcttatc
    25921 accagaagca aaaaaatggg tggaagaaaa tcccggtatg ctcaccaatt accgaaatca
    25981 cgccgatttt atcggtaatt ttggtggtaa tggcacaggt gctgaagttc ggatcagtat
    26041 ggaaacagca tacttgaatc tgcttgcttc ccacagttta acctcttgga agtttgatga
    26101 aaatggaaag ctgattattc ctgaaaatga aaacaatgtt aaagctcgaa aacaacagaa
    26161 agaaaaccaa gcgacccagt tgttttccta tcagttgcaa gaactgaaca acatacgtaa
    26221 aaaactacga ttaagtggcg gccggctgac agttaatgaa caaatttact tagatgacac
    26281 acaagctagg ttagtggtgg aaaagttatc ggtaaaaatg aaaagcgcta tggaagaagt
    26341 tatccgcatc aaccaaagag gaatttttga gctagagaaa aaatggcaag aaggcttgcg
    26401 gcaagcacgt gttgtcgccc cttcgttgac tgaacaggaa atcatagaag cattagctag
    26461 tgtgggcgcc acaaaagaga acttagtcga ttctgcgaaa gaagtctatc tgctacgaat
    26521 caataaggca agacgtatgg gggaggcctt tgatacattg gcgaaggaga tccaagacaa
    26581 aatcaatgaa atcgtggcac gagatcgaga attagcacag caattcaatt aaaggagaag
    26641 tagatgaaac tagaagaatt ggaaagagat tatcgaagtg aacgaagaca attagaagct
    26701 caagaagatg aattgatcca ctttaagaat caaggattac gattgatgga gcaagaggag
    26761 cagcgactgc atcatgtatt aaagaactta gctttagata atgagcctct gtttcaagca
    26821 agacgaagtt attcgaatta tcaggagcat tatcaacaag ccctacaagt agaacgacga
    26881 aaactctatc agcagcaaga cgaactagaa cgttcctatc gtcaaaaaat cagacaaata
    26941 actatggaga aatcatctta tgaatgaatt tttaatgcaa ttactaaaag aattagagaa
    27001 aaatctacat cgatcaaatg tacgttcagt tggtatgggg atatctcagc aaacttgggc
    27061 agaaaaacga ttgacagatc aaaaccacca aatgactgca gaagaagcta ctcaagaatt
    27121 ttctaaccga catgcacact taaaagaatc agtaaaaggg caatttgcag aaaaactaac
    27181 cgaattattc caaagacaag aaaagaaatt aaaacagttt cgttagtaaa gttatataga
    27241 aaaattttgt gaaaatggtt tttcacattt ttacataaat ttgtcttaat atcgtagatg
    27301 taagcttttg aacaccattt attcggaaaa agggagctgt gacaagattt ttgtcgcagc
    27361 tccctttaat tctgattaat ctattagatc aatcatcttt atcgtctgat tctttggtct
    27421 ctggattcgc tctagcataa gctgaagcgg tgttccagta atctgtatag ttttcatcag
    27481 taccgccaac gccgaattta aaggcgcttt tcttcggacc attttttgcc caaagtgatt
    27541 ccactgtttt tcctggcgtt tggatagatt ttttattgac agtaactttt ccagctgttt
    27601 gtcctgtgaa cccagatact tttttcttca tcacatcgga tgataagtca aatttctcat
    27661 cagtgccgaa aatctcaggg tttgcttggt aagcttgatt gattagattg gcaagataat
    27721 tagccgaacg tattcttgct tggctgtcca ttccagtatt gtcatcgtgt cctgcccaac
    27781 tactgatagt gatagatgga gttgagacga tcagccatga atctcggtat tcattggtag
    27841 aaccagtttt tccgacccaa tctgcatttc ctagatttcc gtttaagctt gagatgacat
    27901 ctttgaatgg tgtagtgatt ttttcattga tcacactacg catcatgtca ttcatgatcg
    27961 atgccgttgc ttcggaataa atacggacag aattactttc atgtttataa atcacattac
    28021 ctgcattatc tgtgatggaa tcaatgatat agccttcttc gtagactcca ccatttgcta
    28081 aggcctggaa tccatttgtc tgttgaagcg tcgttacatc tactgtccct aaaggagccg
    28141 attcaacgcc ccaattatca tttgctgggt agttcatctt cgccaagtag tgctcgtaag
    28201 cgtattgttt cgatccaccg gaattcaaca catcttgata taagtgataa gctgggatat
    28261 tgttggacca ttccagtgac tcacggacgg tttggaaggt gttcgaccct tcattggtcg
    28321 cattgacgat tttctcacca gcattttttc cttcttgcca agtcgttgca taatctgata
    28381 cacgggattc tgatccgaca agtcccatat cgattgcagg accgtaaaca agaaccggtt
    28441 tgatggatga accagcttga cgttctgtat caaaggcgtg gttattttga ttctggctgt
    28501 aattccgacc acccacgaag ccatagatac gtccggttct gttatccatc aatacatttc
    28561 cagtctccac ttggctacca gcaccatcat ctaacatgta gccataattt tctacgccgg
    28621 tttgcaatgc agcatagata tctttatcaa tcgtcgaatg gatcgtatat cctttgtttt
    28681 ggatcgtttg ctgtgctttt tgataataag cttcactgac cgatgaatct gacatatcag
    28741 cattatcttt ttctgccaat tgttgtgcaa taatccttgt tgcttcgttc atcacggtat
    28801 aatacaagaa ttctctgtca gtctgttccg cgatttgttg tgggagaaag tcttttgtaa
    28861 gatcataggc tttcgcttct tcgtattgtt ctttcgtaat ttgtccttca cggtacatac
    28921 tgaataaaac aaaatctttt ctggcaagac cggcagaaag atcttctttt agcgcgcctg
    28981 tattcgtgta tggactatac gtgatagggc tttgaggcaa accagcgata taagcagctt
    29041 gcggcagagt cacatccttt gcatctacac caaagattcc tctagcagct tcctggactc
    29101 cagcaatatt ttgcccctta ttattccgac caaatggtga gacattcaaa tacgtagtaa
    29161 tgatctcatc ttttgaaaag tgtttttcta cttgtgcaga aagaacgatc tcgtttgctt
    29221 tacgtttgaa tgttgtttca tcggttagga tctgttgttt gactaattgc tgtgtcaaag
    29281 tggatcctcc agacgagccg atgccagtcg cttctgaaac tagtgctcgt aaaacagctt
    29341 tagggacata accttggtgt ttatcgaagt attcatcttc tgtactgata atcgcggttt
    29401 tcaataaagg cgaaatctta tcagatgaaa tagtcgtccg catcagatct gtctggattt
    29461 ttgatatctc tgtattatct gcataaacaa tctttgacgt ttccgtaatg tttcctagtt
    29521 ctgtctgcag ttctttttta gtcggcaatt gtgtgtcttc gacaagatag gcaaagtagc
    29581 cagcacctat tccgatacct agagctgcga acaacaaaag aagaacagta ataaaaacag
    29641 tcaatgattg aaacactcta aaaataatct taggaacaaa ccatttttct ttttttcttc
    29701 tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctcaact aatggtttta
    29761 tttcgctaat tatagcaaat aataggacaa aaaaacagac aaggctgttt ttgacaggaa
    29821 tttctttttc gaatgagata aaacgtgagg aaatgatctt ttttatgaaa gagttcttaa
    29881 gcttttcttt agggaaagtt tcagttacta tggagaacag ggcaaagaaa aacaaactat
    29941 tttctttgta catcatctta tcaatataca ggaattgttc gaaacaattg cctttcaagg
    30001 aggaatccat ccatgttatt aggctcacac gtcagcatga gcggaaagaa aatgctgtta
    30061 ggcgcggcag aagaagcagc cagttacgga tcttcgacct ttatgatcta tacaggcgct
    30121 cctcaaaata ctagaagaaa agctatcgaa gagatgaata ttgatgccgg aaaagcattt
    30181 atggcagaaa acggactaac aaatatcgtc gttcatgcgc cttatatcat taatttggga
    30241 aatacgatca aaccagaaat gtttccattt gctgtacaat ttctgagaga agagattgtc
    30301 cgtgcggaag ctttaggtgc tactcaaatc acccttcacc caggcgctca tgtaggagca
    30361 ggggcagaag ctgggattgc tagaattatt gaggggttga atgaagtttt gacaaaagaa
    30421 cagacggcac agatcgcttt ggagacgatg gcagggaaag gtacggaaat cggtcgaaca
    30481 tttgaggaat tagcgcaaat catcgatgga gtaaaattga atgaaaaatt atccgttact
    30541 tttgatacat gtcatacgaa tgatgccgga tataatataa aagaagattt ttcaggtgta
    30601 atggaagagt ttgataaggt catcggatta gatcggctta aagtactgca tatcaatgat
    30661 tctaaaaatc ctcaaggttc tcataaagat cggcatgcca atatcggttt tggaacaatt
    30721 ggatttgaca cattgaacaa aatcgtccat gatccaatgt ttgacaacat ttcgaaaata
    30781 ttagaaaccc catatgtcgg taaagataaa aagaaagctt acgcaccata tggaaaagaa
    30841 atttccatgt tcaaagcgca agcattcgat cccgaagtgt tccctgagtt attagcagaa
    30901 gaagtcacac tttaagtttt ctacctgaca gcaacaaaaa aggattgggc agtctgcctt
    30961 gtcctttttt gtttaagaaa caaaggagtg ctttttgcat tcgttttcgc catgctcctt
    31021 ttgccccctc tttttaaatt cagacaaaca gggtatactg atagaaaagt aaaaaacgaa
    31081 aagtttcaca aacaagtagc aaaacgtgat tgaaaggacg aaaagatgct gaacatttta
    31141 aaccccttga tcgaccaact aaaaaagaaa catacatact ttattcttgg catagccgta
    31201 ttatttacaa ttattcggtt gtatttatat agtaaagcaa tctatggaac agatatgtcg
    31261 gcgacctatg atgatcagct gctgattcac tatgctcaaa atattttgga tggtaaatgg
    31321 ctaggggagt acacgactac gacactttca aaagggattt cctacagctt gtttttagta
    31381 cttgcacata aactgcatct ttcttatgca gttttattcg gcatgttcag tattcttgcc
    31441 agtatattgg ttgcactagc gtttaaacca atcgtgaaga acaagtatct tcttttaagt
    31501 atctattttt attttttgtt ttctccgatt agttttacac atgaatattc cactcgaaca
    31561 tatcgtaatg ctctcgtgat cccgactgtt gagatcatta tcgcttgttt actagctatt
    31621 tattaccgca aatataattc cagcaaaaaa ttgctgccgt ggtttttagg gctagctgtt
    31681 tcttttcctt tcttttggtt cattcgcgaa gattccatct ggcttcttcc ttttactgta
    31741 gtcgcgttag gcatcagttt cgttcagatc ttgctaggac aagacttttc tttcaaagaa
    31801 gcaaagaagt taaaagaacg accatttctc ctttcgaaac aagaatggct gaaactttca
    31861 ttgtttttac tccctctagt cggtttctcc atcgtctatc agtggatcga atataaaaac
    31921 gagacaacat atggtattgc agccatcaat gaccgttcga gtggcgaatt tggacgtttg
    31981 atgaaacagc tgatccgaat agatgacgaa acaaacttaa ataaagaaaa cagtgagata
    32041 tgggtctcgc atgctgcatt aaataaagct ttagacgcat ctcctacatt tcatactgtt
    32101 tctactcgta ttgacgaatt gtatacgact catgcgtgga cccaaggcgg aaaagtaaaa
    32161 gaattaccag gagatatcat tttttgggca ttgcgagatg tggtaagcga atctggttat
    32221 taccaaaaca atgcaaaaga aaccaatgat ttttggaaaa aagtcaatca agaattagaa
    32281 gaagcctatc aaaagggaac tatcaaaaag aaaaaagaat tctatctgat gggatcaggg
    32341 gatggaaaga cgatgaatga tctgccaatc gtatttgatt ttctgaaatc aggatatgct
    32401 tatgcatggc gatacaaaga tttccatcaa ggggggaatt atagttttgg gacgcaagaa
    32461 caagtcgaac aggcacaaga attattgaat cttccactaa tggataattg gatcgatagc
    32521 agtgatccga gaacacctga tttccagttg acaaaaacag caaaagtcgc gaatgtcatg
    32581 ataaaaattt accaagttct ggccattccc gtgctgattg cttcaatagc agggatatta
    32641 cttatgatac taggaaccgt atttgcaaaa gctgatcgtg cctattatag ttcctctttg
    32701 attgtagtca tcggattaat actgacacaa ttggtttttc ttttcggtgt ctcgtggttt
    32761 tgttcttatg cgccagaaaa aaaggattat tttttaagtg tctacactgg cgcaggcgtg
    32821 ccattaacgc aattcatcgc agtaacaagc atgctagttt ttagtaaatt gaatctgaaa
    32881 aaagctttat cttccaaata aaaagggaac tgtgacaaaa gtcttgtcgc agttcctttt
    32941 tccgaataaa cggtgctcaa aagcttgcat ctgcggtaat aagctcaacc aaacgtaaaa
    33001 tatgaggaac tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
    33061 acctctgata cacggtgttc cagaagtaac ttcttcggaa ataagccgaa atttcacaaa
    33121 aatttgaaaa gcaattttct taaattcctt cttatttctt gaagctgccc acttctgtca
    33181 caacctcctt tgtgtaaaag aatcagtttc tattcgattt cttctatcgt ttcttgcaaa
    33241 cgctgttcat attttttgtt tcctttatat aaatcaccaa tcgtccatgc caacaggaga
    33301 aggacaagaa caatgattac ataggcgatt tgatcagcca atgctagttg agctgaggtg
    33361 gcttgctcgc cgaagaaact ttcgatttga cctggtaatc ctcgtaagtt caagtaagtc
    33421 agactaatca cagagaacca gccaagaagt tttacaacaa atgaattttt gaatcggctc
    33481 cccatctccg cttggctatt ggtcatcatc agtaaaggaa tcatcgagaa tggcaatgca
    33541 aaagccagaa aaacttggga ttcattcatc aagttattca aagcggtatg ttcttcgatc
    33601 gttccttttg tacttgtaaa caaaacacaa atcaatacag gaatcacaga aagcaatcgt
    33661 gtgactagtc ttcttagcca aatcgggata cgcatatgga tgaatccttc catgatgact
    33721 tgaccggtca acgtaccagt aatagtagag ttctggccag aagcaagaag tgctacagca
    33781 aataaagtag acaagatacc agattttgct acagcaatca agataccatt gctcatagct
    33841 gatggatcag ataaagcttc atacagacca aaaaaggaag gatcttggac agaaccggtt
    33901 ttgaatacag cgacacccat gatcaatagc agtgcattga ctaaaaaagc cattgacaac
    33961 tggatattag agtcccaagt agaaaagcgt accgcttgtg ctacatcttc ttcgtcttgg
    34021 tgatcgatct tccgagtttg agaaactgct gaatgaagat ataagttatg cggcatgact
    34081 gttgcaccaa taatccctaa cgctccagtc agcggagttt caccacctat gacaggtgag
    34141 tctgagaatg tatgactgtt agggataaag ccagcaaata aactgtgcca atcagggtta
    34201 gacaagccaa cttgatagac gaaaacgaat aaaatgacga aaatcaagca gacaaccaaa
    34261 gcttcgattt tacgaaaccc gacttttgtc agcaataaaa ggacgaaaac atctaacaca
    34321 gtgataaata cggcaattac caatggaata tcaaaaagta agtagagggc gattgctgca
    34381 ccaatgactt ctgcgatgtc tgttgccata atggctaatt cagtcaagat ccataaaatg
    34441 acacccaatg tcttacttgt ccgtgctcgg attgcttggg ccaaatccat ttgggtcacg
    34501 attcctaatt ttgctgccat atactgtaaa agcatagcaa ttagactaga catcaaaata
    34561 actgacatca ataaatattg gaaattttgt ccaccagtga tcgaagtgga ccagttacct
    34621 ggatccatat aaccaactgc tactaatgca cctggtccag agtaagcaag aagtgtttta
    34681 aaaaaacttt ttccctttgg tacatccact gttccattta cttcagctaa agataaacca
    34741 tttgtatgtt caataagtcg atgcttatga aattgtttgt tggattgatt attcaagaaa
    34801 aaaccacctt tcctatttat ctacgattac attctacacc tcagaaagaa aaaattaaag
    34861 aaaaagattc ggtatgccta attttttttc gtggcgtgtc ttgctggtta atgaaagaaa
    34921 ttttgttctt ctctaaaaaa accactattt tttgcatgaa actaaagaat acgcctttct
    34981 tttttcaata aactttagta aaataaatca ttgagagcaa ttcggaaata gaaaggagag
    35041 atttatatgg tatcaacagg tattgtcgta cttattgcta ttattgcttt gctaatcggc
    35101 gcagtaggtg gctttttcct agcgcgtaaa tatatgaaag actatttaga aaaaaatcct
    35161 cccgttaatg aggacatgtt gcgatcaatg atgatgtcaa tggggcaaaa accatctgag
    35221 aagaaaattc gccagatgat gcagcaaatg aagaaccaag ggaaaaaata ggttctgacc
    35281 caaagacttt ttgcgggctg attttttcag tccgtttttt atttcgtaat ttgttagact
    35341 attcagacta ttcagcaaaa aagacaagga cagacaatcc tatgctcttg ataaagacaa
    35401 aggaggagta aaatgtcgat ttttagaaaa ttaggttggt tttttcgtca ggaaaagaaa
    35461 cattatttaa ttgggatttt ctccctattc gcagtggcct tggtacagtt agttcctcca
    35521 aaagtgattg ggatcatcat tgatgagatc gccgataaga atttatcaat aaagatcatt
    35581 tcttggtgga tcttactatt gatccttgca gctttcttac aatatctttt tcgttatatc
    35641 tggcggacga atatttgggg aagtgcagct cggttggaga aagaacttcg gcagcagcta
    35701 ttcaaacatt ttacagaaat ggacaatctc ttttaccaaa aatatcggac tggagatttg
    35761 atggctcatg caacgaatga tttgaatgca atccagaatg tagcaggagc tggaatcttg
    35821 acctttgcag attcagtgat tacgggcgga acaacgatca ttgcgatgat tttgtttatt
    35881 gattggcgct taacactgat tgcgctgatt cctctgcctt tgctagccgt tacttcacga
    35941 gtgctggggt caaaactcca cgatgctttt cgtgattcgc aagaagcttt ttctagtatc
    36001 aatgataaga cacaagaaag tatcactggg atcaaagtca ttaaaacatt tggacaagaa
    36061 aaagaagatc tcgcggattt tgaagaaaag attgaagatg caattattaa aaacaaacga
    36121 gtcaattttt tagactcctt atttgatcca ttcattacct tgattatcgg tctgtcttat
    36181 gttgcaacaa ttattttagg tggaaagtat gtcttggaag gcacgatctc gattgggcag
    36241 atggtctcat ttattagtta tatcggtatg cttgtttggc cgatgtttgc gattggccga
    36301 ttgttcaacg tattggaaag aggaaatgct tcttatgatc gcgttaatga acttttgcat
    36361 gaaaagacgc atatcgtgga acaactgggt gcagtgcaga ccccggcaaa aggcgagctg
    36421 aatgtagcta tttcctcttt taactatccg aaagaagaat caccagcatt agaacaaatc
    36481 aaatttgcga tccaagaagg agaaacatta ggtattgttg ggaaaactgg tgcgggaaaa
    36541 acgacaatca tcaaattact gatgcgtgaa tacgatgatt atcaagggaa aatccaattt
    36601 ggcggacgtt cgatcaaaga atattcattg aatgctttat taggatcaat cggttatgtg
    36661 cctcaggatc acttcctgtt ttcgatgacc attcgagata atatccgttt ttctaatcca
    36721 aaactttcag aagaagaagt agagcatgcg gcttctttag catttatcaa caatgaaatc
    36781 aaggaattgc caaaaggtta tgatacctta gttggagaac gtggagtctc tttatctggc
    36841 ggacagaaac aaaggatttc gattgctcga gcattgatca aaaatcctga attgctgatc
    36901 ttagatgatg cactttctgc agtagatgcc aaaacagaag aagctatctt gcataattta
    36961 aaagaaataa gaaaagagaa aacaaccatc attgcagctc accgtttaag cagcgtgatg
    37021 catgcaaaag aaatcattgt tgtcgatcaa gggaaaatca ttgaacgagg aacccaccac
    37081 gagctgcttg cattaggtgg ctggtatgaa cgaatgtggg aaaaacagca attagaagcg
    37141 aagatagaag gaggcgaggc ctgatggatg aaaattatca atcggaatgg acaaaatcga
    37201 ttccacttaa agaacagaag aagatcatca aacgactgat gaagttcgcc aaacctttta
    37261 gaagaacgtt catcgttgct atcttatttg cgtttgccct gtctgtgatc aatgtcctat
    37321 tgccaaggat catccaaact tttatggatg atcatctggc taaacagtca gctacgacac
    37381 aagttatttt gttttttgct ggtgtgtact tgtttggagt catagtcaaa agtatcattt
    37441 ggtttttcca atggtatttg tattccatgg cttcattgaa gacctatcag tatgttcggg
    37501 taagattatt tgaaaagctg catacactag gtatgcgtta ttttgaccag acaccggcag
    37561 gatcgaccgt ttctcgagtg acgaatgaca cagaaacgct atttgaattt tggtatgtct
    37621 ttttgatggt attgactggg atctttgcag tcgtttcttc tttcgttgcc atgtttcaaa
    37681 tcaatggaga aattgccttg tggactttgg tgttcttgcc gattttagga atcgtcatct
    37741 ggtattacca gaaattcagt tctcgtattt accgaaatat gcgggaaaaa ttaagtcagc
    37801 tgaacacgaa attgaatgaa tcgatttccg gcatgcgtat tatccagcag tttcgccaag
    37861 aagctcgttt agctaaagaa tttgaacaag tgaacaacga gtatttagac acgcgctatg
    37921 cgatgatcaa aacgaattcg attctgttaa gtccgatcat caattttctc tatgcattgg
    37981 cgattgcgtt ggcgttgact ttgtttggga tcgatgcgtt gcattctccc gtagaagttg
    38041 gactgatcta tgcatttacg acctatgtcc agggattttt taatccaatg tctcaaatga
    38101 tggacttttt gagcaccttt acagatggga tggttgccgg tagtcgtatt ttgaaaatca
    38161 tggatacaga agaatatgca ccgcaacaaa atcctggagc agatcaagcg attacttcag
    38221 gtaagatcga gtttcgcaat gtaagctttt cttatgatgg agagcacaat gtattgaaca
    38281 atatctcttt tgtcgctaat ccgggtgaga ccgttgcatt agtggggcac accggaagcg
    38341 gaaaaagctc gatcatcaac gtattgatgc ggttttacga attttatgaa ggccaaatct
    38401 tgattgatga tcatgacatt cgagaatatc caatggaaga gttacgtaag aaaatgggat
    38461 tggtcttgca agatgccttc ttgttttatg gagatattgc aggaaatatc cggatgatga
    38521 atccagctat cacagacgaa cagattcgcc atgcagctga gtttgttcaa gcaaacaagt
    38581 tcatcgatga attaccaaaa ggatatcact caaaagtgat cgaacacggt gccagctatt
    38641 ctagtggaca acgacagctg atcacttttg cgcgaacaat cgtgacagat cctaaaatac
    38701 tagtattgga tgaagcaaca gcaaatatcg atactgaaac cgaaggattg atccaagaag
    38761 gattggcaaa aatgcgacaa ggacggacaa cgattgcgat tgcccatcgg ctgtccacga
    38821 ttcgggatgc agaattgatt ttagttttag acaaaggtca tatagtagag cgtgggaatc
    38881 atgaaacact tctagcgcaa aatggtttgt atgcagatat gtacaaactg caaagcagtg
    38941 gtgtgtaaaa agaggttgtg acagtagcat agtagcattt tgtcctaagc gctagagtga
    39001 aaataaaaca gagcttccca gattcttcat ctgtgagcgt tagtggcgta gtcacccgat
    39061 ttaaaaaaga cgacgacagc tagtcaaaag tgcctaatag ccactaagaa agcgttgaca
    39121 aaagcgtttg ctttcgataa tataaagaaa acgatacaac ttggatagtg attgcctgat
    39181 gcaagctaaa cctgatgagt ctcccatggt tccatggacg gctttttggg tttttctttt
    39241 tgttttgaat acagccctat atatcatgga aaggagcaaa acatgctgat tgagaaagta
    39301 ctgaataaca acgtcgtaat aaccagggat gaaagaaatc aggaaatgat cgtgatggga
    39361 aaaggtttgg cattcaaaaa gaaagtgggt gacccgattt ttgactcatg catcgacaaa
    39421 aaatttcagt tgacaaatga cgggctttca caacagttcc aagaattggc acaagcgatt
    39481 cctcttgagt acttagaaat ttcttgtgaa atcattgaat atacaaaaga ggctctacaa
    39541 gtccagttga atgattctgt gtatattgca ttgacggatc atttgtatac ggctatcgaa
    39601 cgtcacaaaa tgggaatatt tgtaccaaat gtattgctgt atgatatcaa acaactattt
    39661 cctaaagagt atgccatcgg gaaaaaaaca gtgaagaaaa tctcggataa ataccacatc
    39721 aagttatcag aagatgaagc cggatttatc gcattgcatt tagtcaatgc acaaggcatt
    39781 caaagccaac aaaacaatac ctacctgttt actataacaa tacaggatat tctaaatatc
    39841 gtccgctatt actataagat tacatttgat gaagattcag tccatttttt tcgattcgtc
    39901 acacacttac gtttttttct gacacgcgtc ctagatggag aaaaatcaga agtgggagaa
    39961 gtttctgagg aattgtttca ggttattcaa aaaaaatata aggatacgtt tggttgcgtt
    40021 gagaaaatag cagacttttt agccgagacg ttcaactacc agatgtccac ggacgaaaaa
    40081 atgtatttaa cgatccatac tgcgcgttta gtgcatgaga atcgttgtaa ataaataaaa
    40141 aaaggaggat aaattaacaa gaacgtatga aataagggta gtgacattca gttggctaaa
    40201 ccttccacta atatattgga gggaatagaa atgggaaaat accaagaatt agcgagaaaa
    40261 atcgtagaaa atgtcggtgg gaaggaaaat atcaatggtt tgacacattg tatcacaagg
    40321 cttcgtttca aattaaaaaa cgaagaaaaa gcaaatgatg agatcttgaa aaatatggac
    40381 ggtattgtaa cggtaatgcg agcgggaggg cagtatcaag ttgtcatcgg gaatcatgtg
    40441 ccagtcgtgt ttgaagaggt tataaaagca ggaaacctta cttttgatga ggctgtttca
    40501 acagaaaaaa tgagaccgtt tgatatgctt attgatggaa tatcgggctg tttccaacca
    40561 ttccttgcta tcttagctgc tggcgggatg attcgtggac tgacagcatt ccttgtcttc
    40621 ttaggcgcgt ttgaccgcgg ttctggcaca tttgttatgt ttgataatat cggtgattct
    40681 gtattccaat ttatgcctgt tattatcggt ttaacagctg cccgtaaatt taaagtaaat
    40741 gaattcgtag ggatgttgct tggcgcagca ttaatgaatc ctagcttgtc gctggaagct
    40801 ttgagtggtg cagcagaagc accactaacg accatatttt ctggcactat ttttgaagca
    40861 cctatttatc aaacagtatt cggcattccc tggattgcgc gtaactacgc ttcttctgtc
    40921 attcccatta tctttattgt gttgttagct agccaaattc agaaaccaat taaaaagtta
    40981 gtacctgaga tgatcgcgaa tttcttcgtt ccattcttca ccgttcttat tacaatgcct
    41041 ttaggttttt tactcgttgg accagtattc acattcgcaa ctgatatttt aatggctgga
    41101 tttgaaacac ttttagcact ttcgccagtt atttatggag caatcgtagg cttcttctgg
    41161 caaattctgg tgatgtttgg tctccattgg gcaatcgtac caatgggatt gatgcaattt
    41221 tctgtgaatg gttggcaaaa tattatgaca cctgttgcag tagtgtcttt tggacaaact
    41281 gcagcactta ccgcactcta ctttaagctt agaaatccaa aagacaaagc aattgcaata
    41341 ccagccattg tttctggtat tgtggggatt acagagccag ctatttatgg gtttacatta
    41401 ccacgtaaaa aaatatttat ttttacttgt gtcggtggtg cgttaggggg cgcgtatagt
    41461 gggttaatga atttaacttc ttggaatcaa ggtggactag gcatctttac aattcccaac
    41521 tatattagac cagatggtga tttaacagat gtgattaatg tattgattgg gatcgccatt
    41581 gcgatggtgg tatcctttgc gctgactttt ttcttttgga aggaggaagc aggtgaaaca
    41641 gatgacagac agaaaagatc aggtaaagaa attgtaaaaa caccgataca aggacaaatc
    41701 gctccactga aagcagcaaa ggatgcagct tttgcacaag gaacactagg tcgaggaatc
    41761 ttgatctacc ctgaaaaagg agaagtacgt gccccttttg acggaacagt catgaccctg
    41821 ttccccacga aacatgcaat cggaatggtt tctgaaacag gtctggaatt gctgattcat
    41881 gtcggattgg atactgttca gttggaaggg aaatattttg aaagcttggt gcagcaagga
    41941 gaaagagtga aacaaggcga tctgttgctc cgcttcgaca aaaagaaaat cgaaaaggaa
    42001 ggatacagtt tagaaacacc tgtaatcgtc accaattaca tggattattt ggatattttg
    42061 gaaaatcaag gggagacggt cgggccagac gatgcactta tcaccgcact gacctgaacg
    42121 gttaggtgaa agaatggaat agacttaact attcttttta gtccacttct ttattttcaa
    42181 aattgaaagg ctctttcatg atggctgagc tgatagggcg tttatcaaat ttttgtccta
    42241 cagaaattag atggactaaa ttctgttgat aattttctgc aaaaacacaa ctatataaca
    42301 cgtctagaag ataacaaatc gatgtattga tcgtaaaatt accaattttt gaatacagtc
    42361 gttcgcgtgt cgtaagtcgc aaagcggctg tactcattga ggatagcgtt gaatcaccga
    42421 tactggtcaa cgctaaaatt ggaacatgat tttgtttcaa tatttctgcg gtacgaagga
    42481 tcacattgtt ttcacccgta taagaaatca gaatcccgca catatcactc gtgcaattgt
    42541 acgcttcata gaacgattca ccaaaagtgg tgacaatcgt gacatccatt ttgatgcgct
    42601 tcattttcag catgaaatct tgagaaatca aagtattcga gttactggcg aaaatcacga
    42661 ttttttttgc ttgaagcatt agttgtttgg ctttttgcag ttggtcatga tgaatgagag
    42721 aaagtgtatc ttgaatcgtc atctgttcta aagaagccat tttatttgcg atcgtcataa
    42781 taccgtctgt ggtggaaaaa ggaagattcg catctacgtc ttgaaaattc ccttcaagat
    42841 aggctgtttc agctaaaaaa gcattacgaa aactgaccca gccatcaaaa cccatttttt
    42901 ttgctacacg gatgagtgtg gacgggtgga cataacatcg ctcagcaatc tcttggatgg
    42961 tcatttcgcc aagtgctgga gaatcgctta agagaaactg aatcacttca ttttctgaag
    43021 gagaaaagga gatttctttg attttttcac gtaataacat gtagatacct tcttctagtt
    43081 tttttacgta aattataact tttttgaaaa tgatttgtaa acggatttat gttatatagc
    43141 ttaataaagt cattttttga caagatattt acaaaaatcc agtagaaaac actaactttt
    43201 gtaaatgtta ctggaaagaa acgaatttct attgtttgtt ttgtttacaa aaaagataat
    43261 aaaagtgtaa aaagcaaagg agtgagaaca tggcaaaagg aatcaaaatc gctacgattg
    43321 gtggaggcag cagttacacc cccgagttga tggaaggatt tatcaaaaga tatgaggaat
    43381 taccgatacg agaaatctgg ctcgtagatg tagaagaagg aaaagaaaag cttgagatcg
    43441 tcagtcagat gtctcagcgt atgtgggatg cttctccgta tgaagtcaag atctatgcaa
    43501 ctttggatcg ggaagaagca ttgaaagatg cggattttgt tacgactcag tttcgtgtcg
    43561 gtcaattaga tgcacgaatc aaagacgagc ggatccctag ttattatggg atgattgggc
    43621 aagaaacaaa tggtgctggc gggatattca aagcttttcg taccgttcca gtggttttgg
    43681 caatcgttga agacatgaag cgtttatgtc caaatgcttg gctgatcaat ttcgcgaacc
    43741 caagcgggat ggtgacagaa gcagttatca gatatggcaa atgggataaa gtgatcggat
    43801 tatgcaatgt gccagtaatg gcgacgatga cagaaccagc aatgcttggc aagaaaaaag
    43861 aagagctgat ctacaagttt gccggtctaa atcacttcca ttggcacaaa gtagcagata
    43921 ataaaggaaa tgaccgcaca gcagatttga ttgatcgttt atacgatgaa gacaatggat
    43981 tgccgaaaaa tatctttgac atcccgtttt tcaaagaaca attaaaacaa atgaacatga
    44041 tcccttgtgg ctaccaccgc tattactacc gtgaagaaga aatgttggaa catgctttag
    44101 aagaatatca aacgattggt acaagagcgc aacaagtgaa agaaacagaa gcagagctgt
    44161 ttgaattata caaagatcca gaattggatc ataaacctga acaattgcaa aaacgaggtg
    44221 gtgcttatta ttcagatgct gcctgtgaaa cgattgcttc gatctatgga aataaagaaa
    44281 cacagatcgt ggtgtctaca aagaatgatg gagctgttcc tgatctgccg gcagattgtg
    44341 tagtagaagt cactgcctat gttggcgcac aaggcgcacg aaatgtagcg tttggttccc
    44401 ttcctgctgc tgaaagaggc tggttgcaag taatgaagaa tatggaattg ctgacgatcg
    44461 aagcagccgt gactggtgat tatggaacag ctttacaagc gtttacgatc aatccgctta
    44521 tacgatcagg aaagacagca aaacggatca tggatgagtt gtttattgca catcagcagt
    44581 atttaccgca attcaaggaa acaatcagcc ggttaaaagc agaaggaatc gaagtccaag
    44641 atgaactggc aagagaattg tcaagcaata cacatgtaat ggaggaagta tgatgggatt
    44701 cagaaaagat tttttatggg gcggtgctac agcagccaat caatgcgagg gtggttatga
    44761 tgaaggcgga cgtgggttag cgaacgtaga tgttgtgcca atcggagaag accgttttcc
    44821 tgtcattact ggaaaaaaga aaatgtttga atttgataaa gatcattttt atccagcaaa
    44881 aattgcgatc gatatgtatc atcattataa ggaagatatt gctttgtttg cagaaatggg
    44941 gttcaagact tatcggatgt ccattgcttg gagtcgtatt tttccaatgg gcgatgaaaa
    45001 tgagccaaat gaatctggtt tgaaatttta tgaggatgtc tttaaagaat gtcaaaagta
    45061 cgggatcgag ccgcttgtga cgattacaca ttttgattgt ccgatgcatt tgatcaaaga
    45121 atatggtgcg tggcgttcta gagaactcgt tggtttttat gaaaatctgt gccgagtcat
    45181 cttcaatcgc tataaaggac ttgtcaaata ttggttgaca ttcaatgaga tcaatatgat
    45241 ccttcatgca ccattcatgg gcgcagggct gtattttgaa gaaggagaaa atgaagaaca
    45301 aatcaaatat caagcggcac atcatgagtt ggttgcaagt gccatcgcaa cgaagattgc
    45361 tcatgaagta gatccggaga acaaagtagg ctgtatgctg gcagcaggag catattatcc
    45421 atacacttgc aatcctgaag atgtttgggc ttctagaaaa tcagatcgag aaaattattt
    45481 cttcattgat gtccagtcac gaggagaata tcccgcttat gcattgaaag aaatggaacg
    45541 aaatggcatt cagatcaaaa tggaacctgg ggatgccgca ttgctgaaag aacatactgt
    45601 tgattttatc tccttttctt attattcttc tcgagtttcg actactgaca acgaactctt
    45661 ggaacagact gcaggaaata tctttgcttc tgtcaaaaat ccatatctgg aagcaagtga
    45721 atggggttgg caaattgatc cattaggctt gagaataacg atgaatgata tttatgatcg
    45781 ttatcaaaaa ccactattta ttgtagaaaa cggcttaggt gcagtcgata cgcccgatga
    45841 gaatggttat gtggaagatg actatcgaat cgactatctg gcagcacata tccaggcaat
    45901 gaaagataca gtagaattag atggagtcga tctattaggc tatacgactt ggggatgtat
    45961 tgatttagta tcagctggta caggtgagat gaagaaaagg tacggattca tttatgtcga
    46021 tcgtgacaac gaaggaaatg gcacattagc ccgttcgaag aagaaatcat tcaactggta
    46081 taaaaaagta attgattcaa atggagaaaa tctttcgaat gattgatcaa tgatttgtaa
    46141 aagtctattg tttttcagcg gatttttcga gtataataaa gctattcgag aaacgcaagc
    46201 agaacaatag actttttttc gcattggctg taaagtatgc ttacttgatt aagtaaagga
    46261 gaatctacat atgtatgatg taatcgttat tggcgcaggt cctgctggga tgactgctgc
    46321 tttatatgct tcacgttcaa acttatccgt attgatgatc gaacaaggcg cccctggcgg
    46381 ccaaatgaac aatacggcag aaatcgaaaa ctatcctggt ttcgattcaa tcatgggtcc
    46441 tgatctagct tataagatgt atgaaggtgt ttcaagattt gggacagaaa atgcttatgg
    46501 aatcgtgcaa gatatcaagg atcacggcag ccacaaagaa gttatttgtg aagataaaac
    46561 ttacgaagga aaaacagtca ttatcgcaac aggctgtatc caccgtaaat taggcgtacc
    46621 tggcgaggaa gaatttgctg gacgtggcgt ttcttactgt gcagtttgtg atggtgcgtt
    46681 tttccgtaac aaacgtctag tagtgatcgg aggaggagac tcagcagtag aagaagccat
    46741 ctatttgaca caatttgctt ctgaagtagt catcgtccac cgtagagatg aattacgtgc
    46801 acaaaagatc atccaagacc gtgcatttgc gaatgaaaaa atttccttta tgtgggatac
    46861 agtcgttgac gaaatcgttg ggaatgaaat ggtagtgaca ggtgtgaaag cacgtaatgt
    46921 caaaaccggc aatgagtttg atttggcagc agacggagta ttcatctatg ttggactgga
    46981 tccattaact gcaccattta gagaagcagg gttgacgaac gaagctggct ggattccaac
    47041 agacaacgaa atgaagacga atataccagg cgtttttgcc atcggagatg ttcgtgaaaa
    47101 agatttgcga caaatcacta ctgctgttgg tgatggtggg atcgctggac aacaagtgta
    47161 taaatacttg gaatctttag aagaaacaac tgcataagtt taaaaagagt ggaagaacgg
    47221 tttcaattgt tcttttactc ttttttttcg cgtgaaatct actgaatccg atttatgcga
    47281 atacttgcaa ccgtgcttga taaaagaatc ctttcttgat ttaagcgttt tttagacctc
    47341 gtactttcat atctaataag tgaatgctat acttattaag aaccatattt cagaaagagg
    47401 tgttattttc atgtcttggg aacaagtgta tgaacaatgg gtacatgaag aaaatttaga
    47461 agaaaactta aaaaaacaat taattgatct gggtgaagat cctgaaaaac ttgaagatgc
    47521 tttttatgca ccgttggaat tcggtactgc aggcatgcgg ggaatcttag gacctgggat
    47581 caatcgaatg aacatctata cggtaagaca agcaacagaa ggacttgcac gtttcatgga
    47641 tacacaaggt gaagaaacaa aacgccgagg tgtcgctatc gcttatgact cacgccatca
    47701 atcaccagaa tttgccatgg aagcagccaa aacattagca catcacaata tcccttctta
    47761 tgtattcgaa agtttacgtc caacaccaga gctttctttt gcagtccgtc atttgaagac
    47821 gtttgcggga atcatggtaa ctgcatccca caatccagca gcttacaacg gatataaagt
    47881 ctacggagaa gatgggggac aaatgcctcc tgcagacgca gatgcattga cgaaatatgt
    47941 ccgtgaagtc agcaatccgt taaaagttga agtattatct gacgacgaag ctgaacatag
    48001 cggattgatc acgatcatcg gagaagaagt ggatgccgct tatctagaag aaatcaaagc
    48061 agtaacgatt gatcatgaat tggtggaaac aatgggtaaa gatttgaaac ttgtgtacac
    48121 accacttcac ggaacaggaa aaatgttggg cgaacgtgcg ttgaaacaag ctggattcga
    48181 acaattcgta ctagttccag aacaagctgt cgcagacccg gatttcacta cagtcaaatc
    48241 accgaatcca gaagaacact ctgctttcga gtatgcgatg cgacttggcg agaaagaagg
    48301 cgctgactta ctgattgcaa cagatcctga tgcagaccgt ttaggcgcag ccgttcgttt
    48361 gccaaatggc tcctatgaag tattgacagg gaaccaaatc ggagccctgt ttgctcaata
    48421 tattttggaa gcacaccaat cgcgaggcac tttacctgaa aatgcagcgg tattgaaatc
    48481 aatcgtttct agcgaattgc ctacagcaat tgcggaacac tatggggcta ccatgttcaa
    48541 tgtattgaca ggattcaaat tcatcgctga aaaaattcaa caatttgaag aagatcattc
    48601 ccatactttt atgttcggat tcgaagaaag ttacggttat ttagttaaac ctttcgttcg
    48661 cgacaaagac gcgattcaag cattgctctt attagcagaa gtagcagctt actataaaaa
    48721 acaaggcaaa acgttgtatg acggcttgca aacgattttt gaaacatatg gctactacgc
    48781 agaaaaaacg atttctgtga caatgagcgg acaagaaggt tctgcaaaaa tctctgcttt
    48841 gatggaaaaa ttccgtgaag aagcaccgac agaatttgcc ggagcaaaag tcatccaaac
    48901 agaagacttc aaagagttga cgagaacaga ccgtgaagga aacgtagaaa aactaacgac
    48961 accgccatct gacgtattga agtatacgct ggaagaaaat ggctggatcg ctatccgtcc
    49021 ttcaggaact gagccgaaaa tcaaatttta tatcggtgta aaaggaaact cttcagaaga
    49081 cgcagctgaa aaagtagccg cttatgaagc gattatcaaa aaaatgacag aagaataaaa
    49141 aaacttgaga ctgatggaaa aagtggtaaa ctttagaagt cagtcttttt tcaagttgga
    49201 ggtgaatgga ttgccagaca cgcaaaaaac aaaagtagag aaagtcagcc aactattcaa
    49261 aatgttgagt gaccccactc gtctcaaaat tctgctgtat ctgaaagacg gggaacaaaa
    49321 tgtgacagca atcacccaag cagtagaaat ggaacaatct gctgtatcgc atcaactgcg
    49381 tttattaaga gaaaaccatg tcgtcaagtc ccatcgtgag ggaaaagcga ttttgtatag
    49441 tcttgatgat tctcacgtac tggatatttt gaaccagacg ctcaaacatg tggaacatca
    49501 ataacgatag gtgcaaatag agacaaactg gactgtatca acgaatcttc ttcgctgaca
    49561 cagtccagtt tttttaagaa aaagacaaga aaaactgtta gtctgtttct tcggtggttt
    49621 cagttccata ataactatga tcttctaggt ctaaagcatt caaaataagt gaagctgttt
    49681 cttcgataga taaagaagcg acattgatta ctacgcagcc caatttttca taaagatcat
    49741 tcgcaaaatc gagttcagct cgtatttttt cgatatcgga ataagcagta tcaggattta
    49801 atccgtaaga gcgcatccgt tctttacgga tattgtttaa aatatctggg ttattcgtca
    49861 gtcccacgat ttttttagga tcgatctccc aaagctgttt gggaatatgt gcttgtggga
    49921 tcaaaggtag attggcaacc tttaaatttt tgttagctaa aaacaagctc aaaggcgttt
    49981 tggaagttct ggatacgcct agcaaaacga catctgcttc tagaaaccca cgaggatctt
    50041 tcccgtcatc gtattttaca gcaaattcca ttgctttgat ccgtttgaaa tagttctcat
    50101 tcaataaatg aagggcgcca ggttgccgaa tgggcttgat accagttaag cgctcgattt
    50161 ctgatatcgg cggagtcatc acatcaaaac taaataattg atgttcttca caaaaagaac
    50221 gcgtcatctc gatcaattct tgattgatca atgtgtgcag gacaagggct ttttctttta
    50281 gtgcatcttg cagtgcctgc atcaatgttt tctcatctgt cacaaatgtt ctgcggaata
    50341 aattgaactc aacggtagga tattgggcca tagtggcttg cgctaatttt gtcgcagttt
    50401 ctccagcaga gtcagagatg acaaaaaaag taacgatttc tgtagaatcg gaagacatgg
    50461 ataaaacctc acttgcattt tttttatgtt tattatacca tactctagaa tgtgtacttc
    50521 gtaatcattt tgatttggag gaataagtca tgaaaaaaca gacattgatt gaacaatcac
    50581 tagaaacatt gaaaaataac ggttttaaat atactaaaaa aagagaagca ttgttaactt
    50641 atttagtgaa gcgcaatcgc tacgtatccg ctaaagaagt ttttgactat atgaatcagg
    50701 aattcaaagg agtaagttac gatacgattt atcgaaatct ccatgatttt gcagaaatcg
    50761 atttactgga agaaaccgat ttaaatggag agatgaagtt tcgttttcat tgctgtcaag
    50821 gggaaatcgg ccatcaccat cactttatct gtacggtgtg cggaacaacg aaagaaatcc
    50881 atatgtgtcc aatgaattat ttcgaagaac agctcggtgg atgtacgatc gaagggcata
    50941 gattcgagat tttcggaaga tgtgaaaatt gtcaataagg atttaaataa aataacattt
    51001 gataatttta aaaaaaagca agatttcctt gacggtcaaa acagcattag atataatatt
    51061 tacaggacag tcttgttttt tgagatatat ttcaataaca attacggtta ctttttaagc
    51121 tcggagggag ggaattaaca tgtcaaaaac agtcgttcgc aaaaacgaat cacttgacga
    51181 tgctcttcgt cgcttcaaac gttccgtgtc aaaagcgggt actttacaag aatctcgtaa
    51241 acgtgaattc tacgaaaaac caagtgtaag acgcaagaaa aaatctgaag cagctagaaa
    51301 acgcaaaaaa ttctagttct gtgatgaatt ggagttggta gtgtgtcact actaagtaga
    51361 ttaaacgatg acatgaaaac agcgatgaaa gcgaaagata aggaatcttt acaagtcatc
    51421 cgaatgatca agtcatctat acaaaacgag cagattaaag aaggtcacga cttgacagaa
    51481 gaagaagaat taacggtgtt gtctcgcgaa atgaaacagc gacgtgattc tttgcatgag
    51541 tttgaagaag caggacgtga cgatttagct gaaaaagtaa aaagtgaaat tgtgatagtc
    51601 gaaaaataca tgccggaaca actttccgac gaagaaattc gacaacttgt tcaagaagca
    51661 atcacacaaa ctggtgcttc atcaatgaaa gaatttggca aagtcatggg tgcgatcatg
    51721 cctaaagtca aaggaaaagc agatggaaac caagtaaatg ccattgtgaa agaactatta
    51781 caatcttaat tttatcattt aaactgcgga caatcattgt ccgcagtttt ttt
//
LOCUS       DAYYGS010000009        51252 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000009 DAYYGS010000000
VERSION     DAYYGS010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51252
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.009"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(52..1557)
                     /locus_tag="ACHA44_000503"
     CDS             complement(52..1557)
                     /locus_tag="ACHA44_000503"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HFM8903824.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLEFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(1649..2239)
                     /locus_tag="ACHA44_000504"
     CDS             complement(1649..2239)
                     /locus_tag="ACHA44_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8903825.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            2418..4064
                     /locus_tag="ACHA44_000505"
     CDS             2418..4064
                     /locus_tag="ACHA44_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8903826.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYFQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            4113..4832
                     /locus_tag="ACHA44_000506"
     CDS             4113..4832
                     /locus_tag="ACHA44_000506"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFM8903827.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            5311..6258
                     /locus_tag="ACHA44_000507"
     CDS             5311..6258
                     /locus_tag="ACHA44_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8903828.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            6270..7190
                     /locus_tag="ACHA44_000508"
     CDS             6270..7190
                     /locus_tag="ACHA44_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8903829.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            7231..8688
                     /locus_tag="ACHA44_000509"
     CDS             7231..8688
                     /locus_tag="ACHA44_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8903830.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            9033..9647
                     /locus_tag="ACHA44_000510"
     CDS             9033..9647
                     /locus_tag="ACHA44_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8903831.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            9640..11364
                     /locus_tag="ACHA44_000511"
     CDS             9640..11364
                     /locus_tag="ACHA44_000511"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8903832.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            11361..12812
                     /locus_tag="ACHA44_000512"
     CDS             11361..12812
                     /locus_tag="ACHA44_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8903833.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(13284..15437)
                     /locus_tag="ACHA44_000513"
     CDS             complement(13284..15437)
                     /locus_tag="ACHA44_000513"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HFM8903834.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(15439..16485)
                     /locus_tag="ACHA44_000514"
     CDS             complement(15439..16485)
                     /locus_tag="ACHA44_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8903835.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            16635..17924
                     /locus_tag="ACHA44_000515"
     CDS             16635..17924
                     /locus_tag="ACHA44_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HFM8903836.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            17937..20639
                     /locus_tag="ACHA44_000516"
     CDS             17937..20639
                     /locus_tag="ACHA44_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8903837.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            20624..21481
                     /locus_tag="ACHA44_000517"
     CDS             20624..21481
                     /locus_tag="ACHA44_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8903838.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(21552..22409)
                     /locus_tag="ACHA44_000518"
     CDS             complement(21552..22409)
                     /locus_tag="ACHA44_000518"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8903839.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            22620..24791
                     /locus_tag="ACHA44_000519"
     CDS             22620..24791
                     /locus_tag="ACHA44_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8903840.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLKLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            24859..25200
                     /locus_tag="ACHA44_000520"
     CDS             24859..25200
                     /locus_tag="ACHA44_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8903841.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(25476..27230)
                     /locus_tag="ACHA44_000521"
     CDS             complement(25476..27230)
                     /locus_tag="ACHA44_000521"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903842.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(27241..28077)
                     /locus_tag="ACHA44_000522"
     CDS             complement(27241..28077)
                     /locus_tag="ACHA44_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8903843.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(28079..28762)
                     /locus_tag="ACHA44_000523"
     CDS             complement(28079..28762)
                     /locus_tag="ACHA44_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HFM8903844.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            complement(28921..30315)
                     /gene="uxaC"
                     /locus_tag="ACHA44_000524"
     CDS             complement(28921..30315)
                     /gene="uxaC"
                     /locus_tag="ACHA44_000524"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8903845.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            complement(30354..31853)
                     /locus_tag="ACHA44_000525"
     CDS             complement(30354..31853)
                     /locus_tag="ACHA44_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HFM8903846.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            complement(31846..33267)
                     /locus_tag="ACHA44_000526"
     CDS             complement(31846..33267)
                     /locus_tag="ACHA44_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFM8903847.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            complement(33327..34319)
                     /locus_tag="ACHA44_000527"
     CDS             complement(33327..34319)
                     /locus_tag="ACHA44_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8903848.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            34551..36749
                     /locus_tag="ACHA44_000528"
     CDS             34551..36749
                     /locus_tag="ACHA44_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903849.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            complement(37207..38079)
                     /locus_tag="ACHA44_000529"
     CDS             complement(37207..38079)
                     /locus_tag="ACHA44_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8903850.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            38379..39353
                     /locus_tag="ACHA44_000530"
     CDS             38379..39353
                     /locus_tag="ACHA44_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8903851.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            39375..40067
                     /locus_tag="ACHA44_000531"
     CDS             39375..40067
                     /locus_tag="ACHA44_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8903852.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            40033..41541
                     /locus_tag="ACHA44_000532"
     CDS             40033..41541
                     /locus_tag="ACHA44_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8903853.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            41615..42718
                     /locus_tag="ACHA44_000533"
     CDS             41615..42718
                     /locus_tag="ACHA44_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8903854.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            42721..44058
                     /locus_tag="ACHA44_000534"
     CDS             42721..44058
                     /locus_tag="ACHA44_000534"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HFM8903855.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            44045..44923
                     /locus_tag="ACHA44_000535"
     CDS             44045..44923
                     /locus_tag="ACHA44_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HFM8903856.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            44920..45843
                     /locus_tag="ACHA44_000536"
     CDS             44920..45843
                     /locus_tag="ACHA44_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HFM8903857.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            45840..46250
                     /locus_tag="ACHA44_000537"
     CDS             45840..46250
                     /locus_tag="ACHA44_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HFM8903858.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            46254..47822
                     /locus_tag="ACHA44_000538"
     CDS             46254..47822
                     /locus_tag="ACHA44_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HFM8903859.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            48062..49480
                     /locus_tag="ACHA44_000539"
     CDS             48062..49480
                     /locus_tag="ACHA44_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8903860.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            49492..50802
                     /locus_tag="ACHA44_000540"
     CDS             49492..50802
                     /locus_tag="ACHA44_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HFM8903861.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT        16516 a         9054 c        10465 g        15217 t
ORIGIN      
        1 cacagtccct ttttttctta gaaatgggaa accactagta tataaatacc attattcttc
       61 aatcaaccgc ttcacgattg cataatcccc ttcataaggt tcatctacac cattgatttt
      121 ttgataggga tccacgcctt ttcccgcaat aacgactgaa tcttgcggag cgctttctgc
      181 taaagcgcgt cgaatcgctt caggacgatc catttcataa tgaacgatca aatctggatt
      241 agtaattgct tcattgattt cttccgcgat ttttttcgga tcttcaaatg ctgggtcatc
      301 tgccgtcaaa aaggcgatgt cagctgtttc tgatagcact tttccaaaat catgtcgacg
      361 ggagattgct ttgtttcctg ggctgcctaa tacaacaatt acacggccat ctggatgttc
      421 atttttggca aattccagca atgttttcaa gcttagataa ttatgtgcat agtccacata
      481 gacatgcgct ccattggatt ggatcaattg atccattcgg cctggtacac gagcttcttt
      541 taatccgtct tgtgcatctt gactggaggc acctgttagt gctgcggcaa tgatcgcact
      601 ggctgcattt tcttggttga atcctccagc cagcaaaata tcataagcag tatcgcagat
      661 agctaagcga tcttcatgag aagataaagt aaaaccgtga gtacctttat ctgaacggat
      721 cacttgataa ttgctttctt ccgaacgacc ataagtgatc gttggaatct ggaatagatc
      781 ggctgtttcc ctaagtaaat caaaatagtc actttcatga tttaaaacaa ctgtttttga
      841 attcaagatc aattgtcgct tgcaatagaa atagtcatca aatgtcggat gttcgatcgg
      901 actgatatgg tcaggcgaga tattcaagaa gattccgaca tctaaagtca agccgtatac
      961 gcgttgtgtt ttgtaagctt gtgaagaaac ttccatcact agatgagtca taccattttc
     1021 aactgcttct gacatcatac gatataaatc taatgactca ggcgttgtca aatgggattt
     1081 gaaatatgtt ttgccatcta acgttgtatt catagtagaa agcaatgctg tttttttatt
     1141 tgttgtgtga tccaagattg cttttgtaaa gtacgcagca gttgttttac cttttgttcc
     1201 agtgaatccg atcactttca atttattttg cggatagtcg taaaaagcca tagcaagaag
     1261 cgccattgct ttacggatat ccgttacgat gattcctact gctgaatttt cgtatggttg
     1321 ttctgataca taatattgaa taccctgttc gattgctgaa tccaaatatt cttctttgaa
     1381 attcattcct ttacagaaaa acaaagtaga agcatcagct tttcttgaat cataacttaa
     1441 ctgaagaaat tcagcttttt ctggtacatc aagatgccag cctcttgttg aaacaaattc
     1501 ttttaataga ttttctttta atagtaaatg acggatgtct tctaaagaga agctcattga
     1561 ttgatccctc gctttattca atttcttggt ttattatagc acactctttt taatatatcg
     1621 tttgtttccg ctatgacaaa atccagcttt attcaaattt tcttgacaca ggggacaagc
     1681 ggatgagtat tttcataaac aatgaactga ctgtaataga taggagaacg aatagtgtct
     1741 ccaacaatcc aaaaacataa taattcaaaa agacgataag actattggta agtaaaagtc
     1801 cttgcggcat tggaagccga aaaaatttgt ggaaaataat cggcggtatc gttataccgc
     1861 catttgaggc cccgactgtg tacaatgtgt aaaccccaag actgaacaaa agacttcctg
     1921 aaatcagtga aaagaaatgt gaggagatca tttccacttt tggaacaatc tctaaaacaa
     1981 caggaaaaag cagacttccc gctacagtct taaaaaatac tgattttcct aataggacag
     2041 cggctagaag cagcatcata agattgatgc tccaaacagt aactgagcgt tggatagaaa
     2101 aggccgtttc aactaaatgt ccgatggcgc ccactccggc ggaagctata tcgtgaggga
     2161 gcaagaacca attcaatcca actgtgataa agaaaagacc taaaaatata acgggtagtt
     2221 tttttatttc ttgtgtcatt gatatctcct ataaatgtta gccataaaag gctgtatatt
     2281 tctttacttt accaaaaaaa cgaaaccgaa ttcaaaaaaa caattgttct tataaaagtt
     2341 atataaaatc gattgccagt ttctgtttta ccaattaaaa tgatagtaat gttttgtaga
     2401 aagttggaat ctgaccgatg gataatcaaa gaaaaccagc attagatcaa ttaacaccgc
     2461 aagaaaaaat ggcacgaggc tctgcttgga tgaccgccag taatatcatt tcaagattgc
     2521 taggcgctgt ctatatcatt ccctggtatg cttggatggg cgaaaatgct aaggctgcca
     2581 atggattatt caatatggga tacaatatat atgcattgtt tctactgatc tctacagcag
     2641 gaatacctgc tgcgattgca aaacaaactg cacgctataa ttcattgaat gaatatggca
     2701 cttcacgacg attatttatc cgtgcattac agatgatggg cgggctaggc ctgttgtttg
     2761 ctttgttcat gtacatcgcc tctccttggc tagctcatgc ttctggtggc ggagaagaac
     2821 tgattccaac gatgcgttct ttgagtatcg ctgtattggt ttttccttgt atgagtgtga
     2881 tacgagggta ttttcaaggc aatcaagaaa tgatgcctta tgcgctgtcg cagattgtag
     2941 aacaggtggc acgtattttt tatatgctat tggctacatt catcatcatg aaagtcatgg
     3001 atggtgacta tgtgacggcg gtcacgcaat caactttcgc ggcatttatc ggtatgctag
     3061 caagtattct tgtccttttg tattttttga agaaacatca agcgtataca tcggcgttta
     3121 ttcactacag cgaaaacaaa ataaatattg caacgaagga attgttgtta gataccgtta
     3181 aagaagcgat tccgtttatt attgtgggtt caggggtaac gatcttcaaa ttagtcgatc
     3241 agttcacttt tatgaagatc atgtctgcat ccaccgagta ttccaatgca cagttactgg
     3301 atcttttttc gatttttagt gccaatccag ataagttgac gatggtagtt attgcattgg
     3361 caacatcgat cgcgtctact gggttaccgt tgattacaga agctgttacg ttgaaagatc
     3421 gagtaggtct ggcaaaattg acaagcggaa atttacagtt gttttcattt tttatgtttc
     3481 cggcgacatt tggtgttatg ttacttgcct atccgctaaa tacattattt tatacaccgg
     3541 attctctagg aagtaatgta ctgatccaag caagttttgt aggactcttc ctaggactat
     3601 atatgctagt ttccaacatg ctgcaaggaa tgtttgaaaa caaggcggca attaattatt
     3661 tggttattgg gtttctagtg aaactccttc ttcaataccc agccattcgt ttatttgaag
     3721 tatacggccc tttattggcg acaatgatcg gatttgctgt gtcttgtacc ttgatcttaa
     3781 agagaatcca tgaagtagcc cgctttaaac caagttttgt ctggcgcagg acattgctga
     3841 tctttattct taccttgatt atgcttttgg cttcgtggat cacaaagatg atttttggtg
     3901 cttttttaaa cgaagatagc aaattccagt ctctattgtt gattcttgta gtagcaggta
     3961 taggggcttt agtgtatggt tatttagctt tgaaaatccg cttggcagaa agactactag
     4021 gaccaggaat agcgcgttta cgacataaat taaaaataaa ataagtgaat cagaagagct
     4081 agattgatgc tcttttgaaa tgggggaaac gcatgcgttt agataagttt ttagcagata
     4141 tgggacttgg cagccgcaaa gaagtcaaag gattgatcaa aaaaggaacg atcgaagtca
     4201 atggacaagt ggtgaaaagc gataaagcgc aagtaaatga attcgaagat gcggttactt
     4261 atttaggaga aatcattacg tatcaaaaag atttttacta cattttgcat aaacctgcag
     4321 gagtcatttc tgctactcag gataatcatg atcaaaccgt aatcgattta ttggaagacc
     4381 aggattatcg ggaagatctt tttccagtcg gccgattaga taaagacaca gagggcctct
     4441 tgattttaac gaatgacggg gtgtttgccc atcagttact atcacctaaa aaacatgtgg
     4501 agaaagaata tctagctgag gttagcggta taatgacaga agaagatatc cgtttatttg
     4561 cagaaggact gattattgat ggcgatgagc agacattgcc ggcaaaactc caaattgatc
     4621 aagtagatca agagacagaa acttcaatta tccgtctgat tttgcatgaa ggaaagtttc
     4681 accaagttaa gcgaatggtc aaagcagtag gaaaagaagt cttatattta aaaagaatcc
     4741 gtatgggaaa attttggctg cctaaagatt tgaataaagg gcaatatcga cccatgacca
     4801 aagaagagct agaacaagtg agagaaaaat aattgaatgg atagctaaaa gaataagcag
     4861 agtagttgta gcgccatctg aaaagcagac ttgtccgtct gactttcgga tggctttttt
     4921 gtgaaatttc ggcttatttc cgtagaagct acttctggaa caccgtttgt tcggaataag
     4981 ggactgtgac aagacttttg ttacagtccc cattgttcct tgtgactcag agttagatag
     5041 taaggacacg atctccctaa tactcaggcc aaaacgctga attcacaacc tctgataaac
     5101 ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat
     5161 tttcccgttt ttttctctaa tactcaggcc aaaacgctga tttcacaacc tctcctatac
     5221 tttgtttcat tttccctata ctttgtttat ttattctcat tattgaaagc gctataatta
     5281 cgttgcgaat gaatcgaaag gggtttttag atgaaaaaac gaaaggggtt caatcatttt
     5341 tttatgaatc tatggcgtta taaagcactt gtcctgatgg ctgtaccggg tatgctttgg
     5401 atgatttttt tcttttacat acctgtaatg gcgaatgtcg tagcattcaa aaattttcat
     5461 atttcagcag atgggttctt ggctagcttg agggaaagtc cttgggtggg ttgggaaaat
     5521 ttcaaatttt tattttcttc ggatcaagca tttctcatca ctaaaaatac aattttatat
     5581 aatgtagcat ttatcctttt gaatctgctg atctcagtcg tatttgcaat cattatgagc
     5641 gagttgagaa ataaacgact cgttaaagtt tatcaaacga tgtccttact accttatttt
     5701 ctatcttggg tcattatcag ttactttgtt tacgcttttt taagtcctga caaaggaata
     5761 ttcaatcagt ggatcactgg acatggagga gaagctgtca attggtataa tgaaccgaaa
     5821 tattggccgt ttatcttggt gttcattggt acatggaaag ggattggtta taacagcatt
     5881 atttactttg catctgttat gggaatcgat ccgacttatt atgaagctgc tatggttgat
     5941 ggagctagca aatggcagca gatcaaacat gtgacgatac cacaacttat tccactaatg
     6001 acgattttaa cgattttagc cgtaggaaac attttccgag ctgattttgg cctgttttat
     6061 aacattccta ggaattccgg tgcattatat gatgtgactc aagttttgga tacgtatatc
     6121 tacaatggcc tgacatccac aggagatttt ggtatgacgg ctgctgcagg actttaccaa
     6181 tcagttgtcg gctttgtctt gttgatgatc acaaatacga tcgcccgacg ttttgatagc
     6241 gattctgcat tattctaagg aggaaaaaaa tgaaaaaaaa gaaagtggag agagtccaag
     6301 tccgctcttt caataagacg gctaattttt tctctaatat aatgattgca ctctttgcta
     6361 tttcctgtat tttgccattt atatttgtta tcgccatctc ttttaccgat gagtccatat
     6421 tgtcgactca cgggtacagt ttttggccag aaaagttcag tatatttggt tatacgtact
     6481 tgttcgacca gatgcaggat aaaattttcc aagcgctttt cgtcactgta cttgttactg
     6541 ttttaggtac actgatcaat agcacggcta cttctttata tgcttatgcg atttcacggt
     6601 caaactttcc attccgtcgt ttttttaccg ttttttgttt aattacgatg ttgttctcac
     6661 cagggatggt ggccaattat cttgttatga caaatcttct tcagttaaaa gatacgattt
     6721 gggcattgat cttgccaatg gctgtcagtc ccttcaatat tattgtgatg cgcacattct
     6781 ttaaacgttc ggtctctgat tcgattattg aatcggcacg gatcgatgga gctagcgaac
     6841 tgcgaatatt tgttcagatc gttttaccgt tagctgttcc aggtattgcg acaatcagtt
     6901 tatttgccgc attgggttac tggaatgact ggttcaatgc tttactatac atccaagatg
     6961 ataagctagt tccactacag tatcttttga tgaaaatcca aagtaatatt caatatctga
     7021 cacaaaattc aggagcagga agccaattga caggcggatt agcctcagtt ccaggagaat
     7081 ctgccagaat ggcgatcgta gtcatctcga cgttgcctat tgcgatcagc tatccatttt
     7141 tccagaagca ttttgtcaaa ggtctaacaa tcggcggagt aaaagaataa agaaacaaaa
     7201 atataaatca taaaattagg aggaagaaaa atgaagttat ggaagaaact agcctttaca
     7261 ggtgtttcag cgttgttgtt gggaacatta gcagcatgcg gaggttctgg ttctaaagac
     7321 caggcagaag ctagcaattc ggacactttg cagatgtatc aaatcggaga taaaccagat
     7381 aatttcgatc aattgatgga agtagcaaac aaacgaatcg aaaaagagat cggtgtaaaa
     7441 gtcaatatca attacattgg ctggggcgat tacgaaaaga aaatgaacgt catcatttct
     7501 tccggggaaa attatgatat cgcctttgcg aacaactatg tttccaatgc ccaaaaagga
     7561 gcctttgctg atttgactga attagcgcct aagtacgctg aaaaagctta taatgattta
     7621 gatgaagcat atattaaagg aaatctggtc aatggaaaat tatatgcttt cccagtcaat
     7681 ggaaatgtct ttgctcaaca agtacttact tttaacaaag ctttactaga taaatacgat
     7741 ctttcaattg atggaatcca atcttacgct gatgcagaaa aagtcttgaa ggaattccat
     7801 gaaaaagaac caaacacggc agcttttgcc attggacaag gcttcaaagt ccaagggaac
     7861 tttgattatc ctttagggaa tacgttacca tttgctattg atctggatgg agatgattcg
     7921 aaaatcatca accaatacga taatgaaacg tatatcggac tgttacgtac aatgcatagc
     7981 tggtacaaac aaggactcat tccaagtgac gctgcaacga gcaataagga ttatccacta
     8041 gaaggaaata cttggctaat gcgtggagaa acacaaggac cgtatgatta tggcgataca
     8101 attttgacaa atgcagcaaa acaagaactt gtctcaaaag caattactgt tccactaaaa
     8161 tctacagctc aagcacaaat ggcaaatttt gtcgtatcaa atacttcaaa aaataaagaa
     8221 aaatcagtag aacttctagg gctgcttaac agtgatccag aattattgaa tgggcttgtt
     8281 tgggggatcg agggagaagc atgggaaaaa gtcgatggtt cagatgataa aatcaaattg
     8341 ctagatggtt atcagccaaa tacacatatg tctgcttgga atacaggaaa caacaagatt
     8401 ctatatacac aagagtctat tacggatgac atgattgcca aacgtgatca atcgattgct
     8461 gatgcagaaa cttcaccgat tttaggattc agcttcaaca ctgacagtgt caaaacagaa
     8521 ttaagtaata tttctaatgt catgaatcaa tatttagatg gattgaatac agggactgtt
     8581 gatccagatg aaacattacc gaaattgaaa gacgctttgg acaaggctgg ctatgataag
     8641 gtattgaaag aaatgcaaaa acaatatgat gagttccgac aagaataata atgaataaag
     8701 acaaaggggt tgtgacaaaa gacgtgtcac aacctcttgt tccgaataaa cggtgttcaa
     8761 aagccagttt ctcggtatta agctaaatat atgtaaaaat gtggaaagcc atattcacaa
     8821 aatttttctt actactcgaa actaagcggc tatttcacaa actcttggta aacggtgttc
     8881 aatcagctaa ccctcggtat tcgctcacaa aacgacaaaa tatgaggaac tatttttccg
     8941 tttgtttctc taataatcag gtcaaggtgc tgattgccca accttttttt gtataatgag
     9001 aaagtataaa cttaatgttt ggaggagaag atatgttagg gaaggttttg gaagagttat
     9061 ttatccggat ctgggtcatt ataaaactaa ccttgtattt ctggatatat acatttgccg
     9121 gcgggatcat ctttggatta ggtgcggcat ggaaaacagt caatgaacta ttttatttgt
     9181 acggattcga gtataaagaa atcacaataa aaagaggctg gaatatatat aaaagaaatt
     9241 ttttaagagg caatctgcta tttagtttat ttttatctgg tactgcttta ttgagttaca
     9301 atttatattt atctgttcaa atcaaagggc tcattttttt agctattgat tttattttac
     9361 tttttgcctt gctctgccta tttgctactt atcaatacag tatgatttta gatagtgaat
     9421 atacgatctc tgttcctaat ctgctaaagc taagttttat ttcggttttt tccagctttt
     9481 cgtcttttct aaaaataatt attggtagcg gtataatttt aggcgtgact tggcaattca
     9541 aaggattgat cctgtttggt gtcattggtc ttttgacggt ttggaatggt acgatgacaa
     9601 cacattggcg tgaagaatta gataaacagt tggaaagtta tgaataagtt tcggattcta
     9661 ttttcaaaag gaaccttact aaatcgtctg ttgcggaaat acgcggttat catgatcggt
     9721 gtaacgatga ctgctaccgt tatttttagt atccatactt gggaacaaaa tcaaaaacag
     9781 gcagaaaaca tgacaagtga tgccgttcaa agcacaagca gaatgctgaa tgataaaaca
     9841 accttgagta ggatcatcaa aaaccagctt gtagggaatt ccgaaaagat cgaaaatgtg
     9901 acgacctact taacaaaacc catagatcag tatctgatgt atgtatacga acagcaaaac
     9961 agtaccgatg aattagtttc tttcccaaat caaatcaaag atctctacgc aaattatgaa
    10021 gaactaagtg cgatatatat cgtgttgaat caactaccgg agtattatga atcttctaga
    10081 gaaaacaaag gtggggaaat aaaaaagggg acaccaaaat tactagacgc cttttatatc
    10141 aagttgccta tcacacaagg aggagctgag tcaggaagta tttttattgg ttttcaaaag
    10201 aaagaactag atatgatttt agaaaatcta acgtcattca atggactctc tttatacatg
    10261 atttctggaa cgacaaatcg actatacaca ttccatgata ataaaatagc taaagaaacg
    10321 tttgaaagac aagaaatgat catcacagag agtctgaaga aaaattcaca attgccgatt
    10381 gacgatctgg aaaaaaacaa ttttgttcaa caccaagaac tgtctgatga ttatcgtata
    10441 ttagctgtac tagataaaaa aagtgtaaaa atcgaaacgg gaaaaaatct tgccccgctg
    10501 atcattggta tcattatttt agacaatata ctgcttatct ttctttataa aacattcaaa
    10561 cgatattcac agcaagtatc tatcgtcatg caggccatgg atcaaacagc agcagggaat
    10621 ttagagacgc ggatcgacat aacaaacacg gaatatgaac tgaaggaact ttcaatcggt
    10681 atcaacgaaa tgttggatag catcaatcag tttgtagaag acatctacaa attagaaatc
    10741 aaacagcaag acgcacatat gcgggcgctt caagcacaga tcaatccaca ctttttatat
    10801 aatacattag aatatatccg aatgtatgca atcagcgaag gaagtgaaga attagcagat
    10861 gttgtgtatg cgttttccgc tttgcttcga aacaatacga atcaagagaa aacgatcact
    10921 ttaaaagaag aactggactt ttgcgaaaaa tatgtctatt tgtatcaaat gcgttatcct
    10981 aatcgagtag cctatcattt tatgatcgat cctgatttgg aaaagatcga agtacctaaa
    11041 tttgtgatcc agccattagt tgaaaattat tttaaacacg gtattgattt tactcgattc
    11101 gataatgcgc taagtgtcaa agtcttgcag gaaggcaaac gggtaaggat catcatcaaa
    11161 gataatggaa aagggatgac tgaaaaacgg ctaaaacaga ttgaagaaaa actgtctcat
    11221 cctaaagtgg aacttcacgg ttctatcggt ctgcaaaacg tgaatgaacg tttacgtgcc
    11281 agcttcggca gcagttatta tatgtcgctt gagaataatg agacaggtgg actgaccgtt
    11341 tctattacat tcaaggaggg atgagcgtgt atcaagtgtt attggtagat gatgaataca
    11401 tgattttgaa tgggttaaaa aagataattg attggcaaag tttaggcttt caaatagtag
    11461 caacagcaga gaatgccaaa gaaggactag ctgtacttga acagcgacag attgatttag
    11521 tagtaacgga tgtgacaatg ccagaaataa atggactgga atttattgaa gcggctcaaa
    11581 aagaacgtca taattttgaa tttatgattt tatcaggata ccaagaattc gattatttga
    11641 aaggaggtat gcaattaggc gccgtgaatt atttgatgaa acccgtgaat aagttcgaat
    11701 tggttgaaag cctcaagaaa atcaaaacac ggttagatca gcaaaatgaa caaaaaaatc
    11761 aacaagaaat ctatcaagaa ctgttattca gtcaatggtt gaatgaagaa ttggatgaaa
    11821 taagtgaaga agaaataata ggacaaataa gtgagaagca gcataggata gtattgattc
    11881 aactggcaag aactcatgat actcttatca atagatggtt gcaagaacgg caacaaatct
    11941 tttactatca acgaaattac ggagaccgtg tattgatcac gttgttgttg gaggcagatg
    12001 aagttgatgg cttctgttgt tttgttcaag aaaactttcc tgatcaggaa tggctgatca
    12061 gtatcgggga agagacagat tctttggaca aaatccctga aacattccaa caagcgaaag
    12121 acaatctaca gttgcatcaa ttttatggag ataaagacga gcatgttttt tacgcagtac
    12181 aagcagaaac aaaagatcaa accatcgatt tttcaagttt cagacgcgtg ctgcaaaata
    12241 aacaattgga agtcgctcaa aaaatgatca ctgatttttt tgaacagttt cagttagcag
    12301 ccatgatgcc ggaagatata cgatattcca gttttttact gtttatggag attcaacggg
    12361 aattgattga tctggaagat gaggaatatt tacaaggaat cgaaaaaatc catcaggcaa
    12421 aaacggtgca agaacttcag cagctacttc tgtcattcat caaaaagcat cagagacaga
    12481 aaaagtacag tgacaatgtg gaaaaagtaa tcgtaatcct gcaccagcat tatcaagaac
    12541 cacttacact caaagaagtg tcagaaaacc tgcatttaaa tgtcatgtat ctaggacagc
    12601 tgttcaaaaa agaaacgaaa aaaagttttt cagcttattt gaaccattta cgtatggaaa
    12661 aagcgaaaca actgcttttg cacagtaatc aaaacatcaa tgagatagca agtgagattg
    12721 gctacaacaa caccacgtat ttctctaaat tattcaagaa aatcgtcggc agatccccta
    12781 aagagtaccg agaaaacagt ggagaatcat aaaaaaaaag ccaatgtaat gaaataactt
    12841 ataaaaaatt gtctagaaat ctaattttgg gctgttctta ttacttgagg caaggcagct
    12901 ttttcatgac ctctcgataa acggagttca aaagccagtt tctcggtatt aagccaaatt
    12961 tatgcaaaaa tgtggaaagc cattttcaca aaatttttct taatactcga aactaaacag
    13021 ctttttcacg acctctccac taaatggtgt tcaatcagct gaccctcggt attagctcaa
    13081 aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc aggtcagaac
    13141 gctgatttca caacctctca ataaacggtg ttcaaaagct tgcatctgcg gtaataagac
    13201 caaccaaacg aaaaatatga ggatctattt ttgcttggct gttcttatta cttgatgcag
    13261 gacagctttt tcacaacctc ttgttaatta tagtttgata aggaaaaagg caaatctttt
    13321 gcttgatatt tcttagggtg agggatgatc cctaattggt aagagatcgt gccgcctttc
    13381 agcaaatctt gatgggtaaa atataatttc gtatattctc tgtccatccg tttgatttta
    13441 tggataaatt gttgctgcgg ccgattaggc gttgcttcaa tcgtcaattc ctttccattt
    13501 gacaaaagca aaacagcctt gtcaattaat ggcataccga taacatattc accggttcca
    13561 ggcgtgacag gataaaatcc cagactattg aagatatacc aagcggccat acttccattg
    13621 tcctcatctc cagggtaacc cattggagaa tcgtcgaagg tctgagtcat caactgttta
    13681 agcagtggct gtgccatttc aggttgtcct atataactga acaagtaagg atagtgaaaa
    13741 cttggctggt ttgaaatagc taattgtcca aattctatcg cggccatctc actcatttcg
    13801 tggatctcaa aaccatatcc tcctgggtca aacgttgggg gctgattgca cagctcaatg
    13861 agtttttctt tatatctctc actgccacca tattttccga tcagtcctga aaaatcgtga
    13921 taaacagcaa agctgctttg ccaagcactt ccttctgcat aatcttgtcc ccaacgatta
    13981 gctgaaaatg gttctctgaa cttgccatct ttatcttttg aacgcataaa tcctgactca
    14041 tagtcaaaga tattttgata gttcaatgat tgctgctgat atttcttcgt catttcctct
    14101 tcttctagta ccttggcaac ttggctaata caaaaatcgc tataagcata gtctaatgtg
    14161 tgattgactg attcgtggta gtgattagga acatagccat atgtcaagta atcttctgtt
    14221 ccttggcgtc cataatttgg atgtttgctt tgaaccgtag cacctttttc cattgccgtt
    14281 agaaattcag gcatcaagtc tcgtcggata tcttttacag cagcatctgc aatgaccgca
    14341 tcgatcaatg tcccaggcat aagtccacgt tcatcaggag acagccattt tggcaaatag
    14401 ccactttctc tatagctgtt caaaaaacct tccagcattt cttcgtattt ttctacagca
    14461 atcaaagaat aaagaggata aaccgtttta tacgtatccc aaaacccatt atttgtatag
    14521 agaacaccag gttttataga tcgactagtc gtatcatagt ggattttttg cccactagga
    14581 tcaatttcat aaaatgtttg tggaaataaa aacacccgat ataaattatg ataaaacgtc
    14641 cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat caaaatagga ctgccattgt
    14701 tcttgactgt gagcaaggta agtctctttg ctttggtttt gttctagttc caggttataa
    14761 gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga tagtttgttt ttttgtctgt
    14821 tcaaaataca acgggatcga gtcgctattt ttgttggaaa cagctaactc atcaattgtt
    14881 aaaggatatt caaatctcaa tataaaataa aaagaaaaat caggatcttc tgaaccagca
    14941 aaatgtgtga tttttccttc cactgtgtaa ggatctataa caaagagctg atggcgtccc
    15001 ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat caatagataa gattccgcca
    15061 tacatgctag gtatcaatgt agaacagatg ccataacgta attgacagat cgacagatga
    15121 gtcgggcaaa atatactttc ttctgggcgg taagaacttt gagcatggaa aagggtgttc
    15181 tcctgtagtt ttccattgat cggcgtaaac agcatatgac tgaaatcacc catccaagga
    15241 cttggttgat gggtaagacg gtagccttga aaaacgcggt catcaggatg aaaccaccag
    15301 ctgccgtttt gatccgtagt ttgcggtgca aaataattca tcccaaaagg aacgccagta
    15361 taaggctggc agttcccatt agagaaattt ggctgattcg ctgttgcatg tctagtatct
    15421 atttgaccga tattcatttt atttctcctt tgttgattgt cttttgatga atgaaactgg
    15481 aaatttacta tcggatggtc gatcttttgt ctctaaccaa gtgaccaaag catctcccgc
    15541 aagcttacct aactgttcaa aatcttgtgc gatggttgtc agtggcgggt caacaagagc
    15601 tgctgcttgg atatcatcaa atccgataat tgaaaaatct tcagggatat gataaccatg
    15661 ctgtctcaag agattcatgg tctggatagc gacaacatcg ttttcacaaa caaaagcagt
    15721 tacttcatac ttagtgatat aagaaagcaa ttcatctaat gtcgcgcttt tgtcatccag
    15781 tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca aggtaaccaa ggtaacgctg
    15841 tcgaacggat tgtggatgag tctgcgtacc aaacaaataa gcgatttttg aatgtccctg
    15901 ttcgatcaaa taagaggcag caagcgcacc gccttggaaa ttatcggaaa gaacggtagg
    15961 aaaaggtaac tcatagattt ttttatctaa tatgacgaca gggaattctt tcaatgaaag
    16021 ctcaaaaagt aagtcaagat gttgttctgt atcaagagca taataaatca atccgtcaaa
    16081 ttcattgatt agctcttttg ctgttttatt ttgtagaaag tcaagcgtcg tcatcatgac
    16141 atcaatctgt ttatcttgca tcacagggtt caatcccttg ctaaaatcac caacagacaa
    16201 atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt tttgtaggag aagcaagaat
    16261 ctgttccgga gaagcggatt taacgaaact gcctttcccg cgaacgcgat aaataagtcc
    16321 taattgctct aattcagtca atgcacgttt ggaagtgatt ctgcttactt tataggtacg
    16381 tgaaagttct ttttcagttg gtagttggct gtctacaggt aattcaccag ttaaaatagc
    16441 cgtttgcaaa tcattaaata ttgtctggta caatggttga gtcatataaa cacctcttaa
    16501 tgatatatca tattcaaata ataccataat ggatgaaaaa gtaaataaaa agatattttt
    16561 tgaaagcgta gacataattg gctgtatgaa gtataatctt taaagatata tcaaatagaa
    16621 agaaggtttt attagtggtt tataaagata taccaaaatc ggtgcagaaa tttatggaag
    16681 atatcacaca aaaatgtggg gatatccatg cggactgggc aaagaatttt acagcagcat
    16741 ttgccaatac tctgttgaca acagtcaaaa agcaaaatga cggaaccacc tttttattga
    16801 cgggagacat tccagcgatg tggttaagag actctactgc acaagttcgt ccttatttag
    16861 tgattgcaaa agaagatgag gaattagctg acatgatcag cgggttagtc aaaaagcaat
    16921 ttttttatat caatatcgat ccatatgcga atgccttcaa cgaagaagct aacggtgctg
    16981 gccaccaaac agatcataca aaaatgaacg attggatctg ggaaagaaaa tatgaaattg
    17041 attctttatg ctacccaata cagttggcct atcttcttta caaaaataca ggaaaaactg
    17101 atcaattcaa tcaatctttt attgaaggta tcaaaaaaat cttggacgtg ttcaaaacag
    17161 agcaagatca tgaacactct ccttatacat ttgtacgaga cacgacaaga aaagaagata
    17221 cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac aggaatgaca tggtctggtt
    17281 tccgtcctag cgatgatgct tgccgttatg gatatctggt accatccaat atgtttgcgg
    17341 tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat actcgatgat aaagaaattg
    17401 tacagcaaac acagaaattg aagaaagaga tccaagaggg gattcagaat cacggacgaa
    17461 cggttaatca aaaagggcaa acgatttatg catatgaagt agatggaatg ggaaacgcca
    17521 cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc accttacttg ggatattgtt
    17581 ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt gttgagtaaa gaaaatccat
    17641 atttctatga aggaaagtac gcaaaaggaa ttggatcctc ccatacgcca gaaaaatacg
    17701 tttggccgat tgcattagct atggaaggaa tgacaacgtc agacaaatct gaaaaagagc
    17761 ggatattgaa tttacttgta gcaactgacg caggaacaca ccttatgcac gaaggattcg
    17821 atgtgaacaa tcctgaaaat tatacgagag aatggttttc ttgggcaaat atgatgtttt
    17881 gcgaattagt catggattac tttgatatta gagtagaaaa gtaggaggta tcgtaaatga
    17941 aaaagaaagt atatatcatt tctcatagcc actgggatcg tgaatggtac atggcttacg
    18001 aacagcatca catgcgactg attgaattga tagacgatct actagagcta tttgaagttg
    18061 atccgtcctt caacagtttt catttggatg ggcaaacgat tattttagat gactatcttc
    18121 aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat caatgaagga aaattgagaa
    18181 ttggaccatt ttatatcttg caagatgatt ttcttatcag ctcggaatcg aacgtgagaa
    18241 atatgctgat tggcatggaa gaaagccgta aatggggaac ccctgtcatg ttaggctatt
    18301 ttcccgatac atttggaaat atgggacaga cgcctcaatt aatgaaacaa gctggtattt
    18361 cagcagcagc gtttggccgt ggtgtaaaac caattggctt tgataatcaa gttttggaag
    18421 cagaaaacta ttcttcccaa tactcagaga tgtggtggaa aggccctgat caaacagcaa
    18481 tctttggtct gctatttgcc aattggtata gcaacgggaa tgagattcca gtagaaaaag
    18541 aagcagctct tgccttttgg aagcaaaaat tagcggatgc agaacaatat gcctctacga
    18601 accacttatt gatgatgaat ggtgttgatc atcaaccggt acaaaaagat atatcaaaag
    18661 caatccatct cgcaaatgaa ctgtttccag attacgagtt tattcattct aattttactg
    18721 actatctgga agcagttcaa aaagatgtgc cagaagactt aggctcagta gaaggagaat
    18781 tgacaagtca agaaacggat ggctggtata ctttagccaa tacagcatct gctcgggttt
    18841 acttaaaaca atggaatacc aaggtccagc gacagctaga aaatattaca gaaccacttg
    18901 cgacgatggc ttatgaagtt agtggaaact atccccatga tcaattagat tatgcttgga
    18961 agacattaat gcaaaatcat cctcacgaca gtatttgcgg ttgttcagtc gattctgtcc
    19021 atcgggaaat gatacctcgt tttgaaaaag cagatgaagt tggaaagtat cttgcacaag
    19081 attccttaga acagctgacc gcagcaatcg atacgaccgg attcccaaaa gacagctttc
    19141 catttgtcat tgtaaatact gcaggaatgg ataaaactgg tgaagcagaa ataaccatcg
    19201 aattagaaag aaaaagattc gctgaaggaa tacctgaaca actatatcaa gagttagaaa
    19261 atttgcctaa acgtaagtat catgtagaaa caaaaagtgg ggccactata ccagctattt
    19321 tatctgaaga aactgtccaa tttggttatg atttgccgaa agaccgtttc cgtgtgccat
    19381 atatggcaag aatgatcaaa gtgactttac cattagaaaa catgccagca ttttcttggg
    19441 aaacattcgc gcttgtagaa ggggaagcga aagcagaaga aaaggaaaca atgattcatc
    19501 agagtggacg aatcatcgaa aacggccctc tacatttgac gattgaaaaa aatggtacca
    19561 ttacaatgga agatcgcaaa aacaaacgaa agttgaataa tctgcatatt tttgaagata
    19621 tcggcgatat aggaaatgag tatatcttta aacaaccttt ctgtgatcag ccaatcctat
    19681 ccagtaacaa agaaaactct gaagtaaaag tattagtaga tacaccggaa atagcaaaaa
    19741 taagtattct acaggaaatg gaaatccctg tttctgcaga cgagcgttta gaaaaagaac
    19801 agcagatggt tgtggaattt cgttatcgaa aagcagaacg atccaaagaa aaacgaatcc
    19861 ttcaaataaa aacaatcatg acaatcagaa aagatagtaa aaaaatcgac tttgaaacga
    19921 ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt gtttcctaca aagttacatg
    19981 tggaacatca cgaagcagat agtatttttg aagtggtgaa gcgcccaaac catgtgtcta
    20041 aaagttggga aaaccccacg aatccgcaac atcagcaagc atttgtcaat attcatgatg
    20101 aagagtacgg tgtaacagtc ggaaactttg gtttgaatga atatgaagta acagaagacg
    20161 gacaaatcgc agtgacgcta cttcgcagtg tgggagaact aggcgattgg ggatattttc
    20221 caacaccaga agcacagtgt ctaggtgagc atcggttcaa ttacagcatt gaactacatg
    20281 gaccagaaga aaaattctct acttatttgc atgcatacgc agcacagatt cctttttcca
    20341 ctcaacaaat caaacatcat gaaggaacat tgatttcaaa acaacaatat ttaacgataa
    20401 aaagtgaaac gtttgcaatt acagctttga aacgcagcaa gtttagtgat aaagtagtag
    20461 taagaggttt caatatgtct agtcatttag aaaaattgga aataactaaa gacaatggaa
    20521 aaactgtcat attgaatctg cttgaagaac caacgaagca agcggttgtc ccaataatcc
    20581 aaccttatga gattcggaca atcggattcg aggaggaaaa cgaatgtatg gcggaataga
    20641 agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt ggagaaattt tagaaaaatt
    20701 aagtatccct accacaacac cagaagaaac attggatcag gtatttgaat tttttgatcg
    20761 ttatgaattg aagggaatcg ggattggctc attcggcccg attggtatcg acaaaacaaa
    20821 agataattac ggatacgttt tggcaactcc caaaaaaggg tgggcacaat tcgattttct
    20881 tggcacaatc aaggaacgct acaatattcc tgctgcttgg acaacggatg tcaatgctgc
    20941 tgcatatggg gaattgaagc aaggagcagc acaaggaaaa aatagttgta tttaccttac
    21001 cgtgggaaca ggaatcggtg caggtgtcgt gatcaatgaa gaaatttttt cagggatcgc
    21061 acatcctgaa atgggacata tttgggtgaa acgccatcca gatgaccatt atgaaggtac
    21121 ttgtccttat cataaagatt gtttagaagg attagcagca ggaccgtcta ttgaggcacg
    21181 cacagggatc aaagggcaag atctgccgca agatcatcct gtttgggcta ttcaggctta
    21241 ttatattgct caagcactga tcaactatac gttgacgtta gcacctgaaa agattatttt
    21301 aggcggtggg gtaatgaatc aggatcatct ccttcaaaaa atccgtcagc aatttgttga
    21361 attgatgggt ggttatatgg aaacacctca agtaagtgaa tatatcgttc gttggggact
    21421 gccgaatgaa agtgggatta tcggtagttt gctgttagca gaacacgaac atcaaaagta
    21481 agcagacaaa ttacacaaaa agaacagagt cttatcatta tctgctgata agactctgtt
    21541 cttttttatt tctattccca taatccaggt aaagctttac cgactatttt ttcttgagta
    21601 aatggatgga tcatgtgcag ttctaatgca tggagcatca gtcgttcagc tgttctattt
    21661 tggtagagag ggtctccaac gatcggatgt cccttatgag ctaaatgaac tctgatctgg
    21721 tgtgtacgtc cggtttccaa aacacaatag acttgtgttt gttcagaaag tactttgttg
    21781 acagtgacat gtgtcaaggc gctctgacca tttttatcat ctacgaccct ttttctccga
    21841 tcatggcgat ctcgtccaat tttattggtg atggtcatat ctttggcaat ttctccccat
    21901 acgatggctt gatatctccg atagatcatt ttttgttcca acattcttcc aagtatcggc
    21961 aagacaaatg gattttttgc aaataaaacg gcaccgcttg tttctttgtc caagcggtgg
    22021 acaacgtaag gtcgttgctg tttagtgtcg agataagcag ctaaatgatt gagtaaggta
    22081 ctgttttctg tcggttcatt tggatgagtc ttcattcctg caggcttatc cactataatc
    22141 aaatgctcat cttcatataa aacacggatg tgcttcgcat ctcctaattg aacttcttga
    22201 taattataat ctgtttcttc gaataacaat gtgatctggt cattgccctt tacttcatga
    22261 tggaacaaag caggttgttg attgatccaa acgtttttac gtatacgcaa aaagtgtcga
    22321 acctttctag gaactaacca ttccttttca agcaagtcgc gaattgttgt ttcaggatgg
    22381 ttcataggta aagtgatcgt gattttcaca agtgtactcc tttcagtgca atcgcagact
    22441 aattgtacca tttttaaaac atatttaaga aaaaacttac atttttctgt tcattcttat
    22501 gttactctac ttgcactaac gtaaaaaaac atattgaact acaacttaag caggaagaaa
    22561 acactctttc actatggtaa aataagacga ttgaatcaaa tagacaggag aagaagcaaa
    22621 tggattttca aagcatttta gaaaagataa aagctgcttt gatcagtttt tggcgttgga
    22681 tcagaccata tctcggacaa ttccatcgat ggcgaaagcg catctggaaa aaatatcatg
    22741 tgaacaaatt gattttgctg attggtttga tctgtgtctt agtgacaagt atctacctct
    22801 ttattttagc aaaacaagca aatgtcgaaa cgttaaaatc aggattaagc caatcgacag
    22861 tcatttatga taagaaaaac gaagaagctg gcacactgta cgcccaaaaa ggaacatatg
    22921 tagaattaaa cgcgatttct ccctatatcc aagatgcagt aatctcgaca gaagatagga
    22981 atttttacga gcatcacggg tttgatatca aagggattgc ccgtgcagca gtccgaatgg
    23041 tcttgtctgg cggaacatca gggggcggag gaagtacgat cactcagcag cttgccaaaa
    23101 atgcgtatct gacattagac caaacgtttg atcgaaaagc gaaggaatta tttttggcaa
    23161 tcgagatcga aaagaagtac agcaaagaag aaatcttgac catgtatctt aacaatgcct
    23221 attttggcaa cggggtatgg ggtgtccaag acgcttccag aaagtatttt ggagtagatg
    23281 caacgaatgt tacgctgtct gaggctgcta ctttggcagg aatgctgaaa ggtcctggta
    23341 tttataatcc gatcgatcac ccaaaaaatg cgaatgatcg gaaaaacaca gtactaagtg
    23401 taatggaaga aaacggaaaa atcacaaaag aacaagaaca agcagaaagc cagacagata
    23461 tcagcgcgta tcttaatgac acatacgata attcagattc tggttatcgt tatccttatt
    23521 attttgatgc agtgatcgat gaagcagtta acgagtatgg tcttgatgaa gaagatgtca
    23581 tgaataaagg ctataaaatc tatacagctt tagatcagga ttatcaaaag caattagaag
    23641 ctacttatca aaacagtgca ttattcccag caaatgcttc agatggtgcc atggtccaat
    23701 ctgcttcagt agctttagat ccgaatacag gtggtgtaca agcactagta ggacgaagag
    23761 gagatcatgt ctttagagga ttcagctttg ctacacagat gcgtcgttca ccaggatcaa
    23821 cgatcaaacc attaagcgta tatacaccag cactggaagc tggatacaag ccttccagta
    23881 tcttgaaaga cgaaccgcaa agttattacg atgcgcataa ttttgacggg acataccaag
    23941 gagaagtacc gatgtatcaa gctgttgcac aaagtttgaa attgcctact gtatggcttt
    24001 tgcatgagat cggtttacaa aaaggatatg ataaagcaga agaattcggc ctgcctttag
    24061 caaaatctga taagtattat ggcttagccc ttggtggact ggaaaaagga gtatctccat
    24121 tgatcatggc gggtgcttac agtgcatttg ccaatgatgg tcagatgtat acgccgcatc
    24181 ttatcacgaa aatcgttgat tcgactggag cggtgatcgt cgataacaca aacaaaaaac
    24241 caaaacaggt catatcaaag gaaacagccg atgaaatgac cagcatgctt cttggtacat
    24301 tttcaaatgg aacagcggct gctgcgaatc ctgcaggtta tacgatagca gggaaaacag
    24361 gaaccacaga aactaatttc gatgccacaa aagcaaatga ccaatggatg atcggttaca
    24421 cgccagacgt agtgatttcg acatggatgg gttacgaaga atccagcaag ctgcactatt
    24481 tggaagggac aagtggaaat gttgtcggga aagtcttcaa atcagcggca gaaggcatcc
    24541 ttccgtatac ttcaaaaaca ggatttagtg tagcagatgc ctatgccaca ggaggacaag
    24601 tggtagctgc agatcaagtt ggaaacacag gaaattctaa tggagagtct ggaaattggc
    24661 aagataactt gaatcaatat ggtgaacaag caaaagaagg gctgaaaaac ttcggcgata
    24721 tggtgaaaga aggcgttcag ggaattggtg aagcggctaa agacctctgg cggaaatatc
    24781 aaggagagta gcccctttga aaacgaataa ccccatgtta caatagagag taactactaa
    24841 aagaaaagag ggcataacat gtcaaacatc tacgatagcg cgaatcaaat cgaacgtgag
    24901 atccgtgaat taccagaatt caaagcgttg aaagaagcgt acgagaaagt acaagcaaac
    24961 gaagaagcac acaaactgtt caaagacttc caagcgatgc aaatcgaatt acaagaaaaa
    25021 caaatgagtg gacaagagtt cagcgacgaa gatgcaatga aagcacaaga aatggctgtg
    25081 aaaatccaat cagaagaagt aatcaatgac ttgatgcaaa aagagcaagg cttcagtaca
    25141 atcatcaatg acttgaaccg tatcatcatg acaccagttc gcgaccttta cagcgaataa
    25201 aactatttta tgaaacaaca gagggtgtga aagagacgtt cggccttgag cgatgacaaa
    25261 attgcttctt attgccgata ggctgtctct tgaacaccat ttaccaagag gtcgtgagcc
    25321 taccgtttaa ctctgagtta caaggaacaa tttcaaaaaa cagcttctca tgtttgatga
    25381 ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcag aaataggagc
    25441 tacgacaaaa gtcttgtcat agctcctatt ttttgtcatt catttttcaa gcggtagaaa
    25501 tagccttttt gagctaacag tttttcgtaa gtacctattt caacgatttt tccttcatcg
    25561 atcacaacga ttttatctgc ctctttgatc gttgaaagcc gatgagccac aataaaagta
    25621 gtgggtgttt ctgtcaggta attcagggct tgctggattt ttttctctga ctggttatct
    25681 aaagctgagg ttgcttcatc tagcaaaatg atttttggct gacgtatcaa cgctcgtgca
    25741 atggagatac gttgcttttg gccaccagac agcttgttgc catgttcgcc tactaaggta
    25801 tccagaccat caggaagctg gtcaatcagt gaagaaagtt gcgcagcttc gatgacttca
    25861 tctaaacgtt cttttgatac gtttttcaag ccataagtga tgttctcttt gatcgaagcg
    25921 gagaacaaca aagtggtttg aggaacaacg gctaaataat ttcgcacact tcgcatatcc
    25981 agttcatcca aaggctgatc atctagaaaa atttttcctg aagtcggctg gataaatcca
    26041 atcaataagt taaggagtgt agattttcca gagccagatg gcccaacaaa agcaattgtt
    26101 tctccagctt tcacctctag agaaaaatga tcgataatcg gtttgagtga gtccggataa
    26161 agaaaagaaa catcctcaaa acgaaaattt ccatccaaat gttggatttt tttcttaccg
    26221 tgataagttt ctacatcgcc agcaatcaac agttcgccaa tagaagtgat agattccatg
    26281 ccttttgaaa tcgtaggaat cagcgtgatc attccagaaa cggaattgac gatcgtcgtg
    26341 aaatacgtct gatacatcac gacatcgccg ggttggatgc gcccttgtaa agctaatatc
    26401 ccagtgaaaa tcaaacaaat cagctgaaat atctgaaaac tagcccagct gactgatccg
    26461 aaaacagatt gcaggatatc taagtggaat cctttttcat agatttgagt aaaatgctta
    26521 tttaagcgag ttgtttcgtg tttttctaac ccatgagctc gagcaatcgg tgctaattcg
    26581 atcacttcga tcacttttgc agaagtctct tccatttctt gcctgaagtc tgagttggac
    26641 tgtctgatcc ttttacggaa aaagagagta acgaatgcgg cgagaggaac agtcaataag
    26701 aaaaacatga aaacaacagg acttttataa agcgtgatac ctaaagctac gaataaattg
    26761 acagcgatat ttaatccgct gacaaatact tgagaggaca acgtttggat agcttctaca
    26821 tcacggatga tctttgactg gattcgtcca gattgcaatt ctttttgata aggaatagat
    26881 agttcttgga ttttcttgat aagggtcgct cttagtccag cttctacttg acggatcgaa
    26941 gtgctatggt attttacatg aaaataattc gtcaatatat tttgaccaat cagacaagcc
    27001 atcaaaaaag cgttgaaata aatggcctgc aactgagaag aatctttcga tgaagctgca
    27061 ttgattacat tggcaatagc gatcggcatt aaccaagatg gaagatgttt gataataaaa
    27121 aagagtcctg acatggcaaa acgccaataa tttccctgat aaagatgcag caatgtctga
    27181 aaagcatttg catgatattt ttgataactt ttctgtattc gttcctccat aatcttctcc
    27241 ttaacaatct tggtaaataa actgcaagtg ttctattgct tcaggaacat aaatctcagg
    27301 agtagcttca agagtggcgt acattagagg acgctcatat ttgatataat gcagtaccgg
    27361 gcggaaatct aaacagccct tgccaacagg taccatgcgg atctttccat tttccatgat
    27421 atagtctttg agatgaagcg ctgtaatata accatgtaaa ttgtccaagg cacctcggat
    27481 cactttttcc tgatcagcat gattttcttt atgcatcaaa ttcgcacaat ctaaaatgat
    27541 ttttaaattt ggtgaagcta cttcatcgat taaacgttta gctaactgat agttatagat
    27601 tggatgattg atccctgctt caacagcaac agttacgcca aaattttctg catgagcgac
    27661 taattcaatg atagaatttc tggcgatctg ataagcttct tcagtaaaat tttcttttgt
    27721 gtaaccgaca gagacactac cggtttccgt agcaatcatc ttggcttgat aggcagagca
    27781 cagagcaatt tgctgtttga aagttgccaa tgcttgtgca cgaacttctt gatttttgct
    27841 ggataggttg atatagcatc ctaaaatgga aagttcgata ttgttttttt ggaagtattc
    27901 accaaaatag gcagcactcc caagtgtcat gtcagttaat ttaagttgtt caggaaatgc
    27961 tttttgtggt gcaaattgga tatgggacaa gtgaagctgt gatacttttt gaacgatttc
    28021 ttcacgattt cgaacatcta aatcgtgggc acggatgcct aaatttaatg gaaacatctc
    28081 aaactccttt ttgtaaataa ttagcaagag gcagacggga ttcttttatc aattcaacaa
    28141 ttattcgggc aactttatca gcgccttctg gactaaagtg ggtattgtct tttagtcctt
    28201 ctgggtaatt tttacaagta tcaggtataa gatgcaggta gaattttttt gtctcttctt
    28261 ttgagaaggt ctggaacaat tcttgtgatc ttgagaacac atctaaaaga ggaatatggt
    28321 gtttctcagc aaatgcacgc atcgcttttg gatagtctcc aagtatatct ggattcaatg
    28381 ttccgttttc taagtaatct cgtcgcgtca cagatgtcag taagaccatt tggacgttcg
    28441 ctttttttgc gcattcaaaa aatatctgca gattttttaa atagctaccg aagggaagcg
    28501 tacctctttc ttcttggatt ttctggtcgt tatgtccaaa ttgtacaaat aagaaatcat
    28561 tcggctgaaa ctgtgaagct agacgggcta accttccttc ctggataaat gatttggtgc
    28621 tgcgtccatt ctttgcttgg ttattgatct ggatctcggg tgaaaaataa gcagagattt
    28681 tttctcccca acctgtctcg ggacgcttgt tttcatcttt ttctgccgca gtagaatcac
    28741 cggcaatatg gatagtaggc atgttatctc ttctttcata aaggtttata ggcaatgaag
    28801 cagaacagca agagaagaag acggagcaaa tttggtccgt cttcttctct aaaacgtgga
    28861 taaacggatt ccgttatttc actatcctgt ttttgattcg ttactgttac tattaatcaa
    28921 ttaggcaaaa aagccaaagt aatttttagc atttgtataa ctaatcgctt gaactagact
    28981 tcctaaaagt tgttcatctt caggtgcttc tcctcgcatg accatttcgc ctaaaaattc
    29041 acaaagaaca cgacggaaat attcatgtct agtgtaggac aggaaacttc ttgaatctgt
    29101 cagcatgccg acaaaattcc ccaaagcact accatcagca aattgtgtca attgatcgcg
    29161 cataccagct cgagtatcgt tgtaccacca tccagaacct aactgcagtt ttccattctg
    29221 ttctttttgg aaacagccca tcaaagctaa aatcgcagga tagtcattcg gattcaatga
    29281 ataaaggatc gtctttggca actgatccgt ttcttctgca cggttcaata atttttgcag
    29341 aggaatagtc aaagaaagat cattcattcc atcatagccg gtatcgggcc ctaatttttc
    29401 caacatttgt gtattacagt tacgataagc atggatgtgc agctgcatcg tccaattatg
    29461 agaatgatag aagtgggtca aatgtatcag tgtttctgta cgataagcat cgatttcatc
    29521 tgaagataat agtttgttag ctcgtgcttt ttgaaaaatc tcttctaata ctgtttcatc
    29581 cgcttcttta tagcgtaaga tgtctaaagc gtggtcggaa acacgacatc catgttgatg
    29641 gaagtattct atccgctgcc ctaagacttc tatcagttct tgataggtgg tgatcgtttg
    29701 gtcagctgct tcttccagta gtgtgaccca tgcattaaat ccttcttttt caatattgag
    29761 tgctttatct ggacggaatg aaggtagaac cttcaaatct ggttcttcat gagcaagcaa
    29821 ctgatgatag tgcaagtcgt ctgcgggatc atctgttgta caaatcactt ctacattcga
    29881 tttttttgcg atttcccgac ggcgaaaatc aggagctgcc aaaatttcat ttgcttgtgt
    29941 ccaaattttc tctgcatttt cttcatttat caacaagtca ataccgaaga aacgcttcaa
    30001 ttcaagatgt gaccaactgt aaagcggatt tccgataatt tttggtacgg tttgacacca
    30061 agctaaaaat ttttcaaaat cagaagcatt tcctgtgatt ttttcttcag gcacgccgca
    30121 agctcgcatc aagcgccatt tgtaatgatc gcctcctaac caagcttctg tcagattgct
    30181 gaaattgttg ttttcgtaaa tttcttttgg atctaagtga cagtgataat caataatggg
    30241 catgtttttt gcgtaggaat ggtaaagctt tttcgcccaa tcgtctttta ataaaaaatc
    30301 atcagataaa aacataaatc ttccttcttt ctttttatac tgttttatga aaatcaaagt
    30361 gttactccgt ttttaaaaat ggcaatttct cgaacgtcat tttgttcatt tttcgtttct
    30421 tctccactag ctacagcgat gattttttga acaaacgatt ctaagattac ttccattggt
    30481 tgttctaata agacgcctgc attaaaatcc atccaatgac ctttgcgttc aaaaagagtt
    30541 gtattcgtag ataccttgac cgttgggaca tatgagccaa acggagtccc tcttcctgtg
    30601 gtaaacaaga ccaactggca atcggatgaa gcaagagcag aggcagctac aagatcattc
    30661 ccgggagctt gcaataaact aagccctggt tttctgattt tttcgccata ttgtagcaca
    30721 tcaacgacag gagagcttcc tgatttttgc gtgcagccta aagacttatc ttctaatgta
    30781 gtgatcccac ctgctttatt accaggagat gggttttcgt aaactggttc cccgtaagac
    30841 aagaaatact gtttgaagtc attgatcaag tcaacgattg agtcaaaaac ttcttcattt
    30901 tccgctcgtg ccatcaatac ttgttctgcg ccaaacattt ctggcacttc tgttaagatc
    30961 gtgctgcctc cttgagcaat caagtagtca gaaaatgcac caagtaaagg attagcagtt
    31021 attcccgata gaccatcaga accgccgcat ttcaaaccga cattcaactt agagagcggg
    31081 actgatatgc gctggtcatt ttccgccgct gttaaaagct cttctaatag agccacacct
    31141 tgttcgatct catcgtaaac atcttgagcc actagaaatt tcactcgttc ttcatcatat
    31201 tcacctaaaa tttttttgaa ttcaggaact gtattgttct cacaacctaa tccaaatact
    31261 aaaactcctc cggcattggg atgatttact gcgtccgcta aaatttctcg agtattttga
    31321 tgatcttttc caagctgcga acagccataa ggatgcttta aaatcgtaat atggtcaaac
    31381 tgtcccagat ctggatgttt ttccttgaac tgtttgacga tcagttcagc aatgccattc
    31441 acacacccaa cggtaggtac taaaaacaaa tcattacgaa tccctacttt tccatttttt
    31501 cgcaagtaac cttgaaatgt tcggttctca aaaaggtaat gacttggatg gacatctttt
    31561 tcatatcgat aatccaattc accagaaaga ttggttttta cattatgagt gtgcagccat
    31621 tgaccggcag ttacgtctgt cgtcacatga ccgattggat agccatattt gatcacgttg
    31681 tctccagctt tcaaatcacc caatgcaatc ttatgacctt gcgggatatc ttcaagagtg
    31741 gtgatcgttt gctgatcaat cgtcagttgt gtttgttttg gaataggggt taatgcgacg
    31801 accacactgt ctcgtcgatt taatttcatt gttggttgca gggtttcaag catggaccat
    31861 cgttcctttc atttcttgta atactgaccg gacacctttt tgatcgatct ttgcagctat
    31921 ttcttgaact gtatctgtca cattcggtat ttgagacaaa tcttctcccc aaagcgcagc
    31981 gttttgcaat aatcgaggaa tagctgttcc attatcagac caagcatcag taaattcgct
    32041 tatcgtcttt tcatcatcat gtggctttac ttgatcgcct cgatacataa gaagtagcgc
    32101 agctagtgaa acagtgatta acggtggtac ctgattttgt tcttcgatat acttcttgaa
    32161 cactggaagc agacgggtgc tgaactttga aacactattt aatgaaatcg cttctaattt
    32221 gtgatgggca aaaggattca agaaacgttc tttgatttgg tcagcataaa tggctaattc
    32281 atctttaggt aaagaaagca taggaatcaa ttctagattg aataactgat caacatatgc
    32341 ggagaataaa ggatctttca ttacttcttc tacagtttcc aattccgcca acaaagcaag
    32401 agggaccatc gctgtatgcg gtccattaag caaatgcacc ttacgttcac gataaggagt
    32461 catatcatct gtgatgatga cattcaagcc agcctgtttg aatggtagtt gattctccaa
    32521 ttctttaggc ccttcgatca cccaaagcat gaatggttct gctttgacca tcaattgatc
    32581 ttcatagtta tgccgttgat tgaaaacacc tgcatcttcg cgaggataac ctgggacgat
    32641 gcgatcaact aaactacaac agaaaacatt ttctttttct aaccactcaa caaaagcagg
    32701 ttctaaatcc catttttctg cgtagcgtaa aatgatttct tttagacgtt cgccgtttct
    32761 gtcaatcagt tcgcaaggaa tgatggtaaa gccagctttg tttaatttga aacgtcggta
    32821 taaaaaggct gtcagtttgc ccgggaagct ctgtggggga gtatctgtta aattatcagt
    32881 ctccacgaat tggattcctg cctctgttgt attggagacg atataggcca agtcgtcatt
    32941 ttctgctaga gatagataag catcccattc atcataagga ttgatcattt gttctactac
    33001 ggtgataatc tcggaagtat caacgatttc gccattgctg agtccttgta aaatgaccgt
    33061 atataaatta tcttgctggt tcatcatatt tccaagacct tgttctaaag gttgaatgat
    33121 tgcaacatta cctttaaaca attgttggtt attcagctgc tgaatctgcc aatcaataaa
    33181 tccacgcata aaattgcctt caccaaactg aagaactttt acaggtgctt cgtttttttt
    33241 cactaattct tttgtcagtt ttttcatata aacttccttt ctgtgttatg cacacgttaa
    33301 catttttata taaaattact tgtagtttac aagtaattga tcgactcacg aaggatcaac
    33361 aaggtggaga gctcaatttt agattgttcg atcgtcctgc cttcgatcaa atctagtaaa
    33421 agctttgttc cttcttgaca catttctttc aaaggcttac gtacggtggt aagcggcgga
    33481 ttgagatagg agacgactgg agaatcatcg aacccgatca gagaaatgtt ctggggaaca
    33541 cggaggccag ctaaatgtac agctctcaat gcaccaatcg ctatgtcgtc gttgccgcaa
    33601 aaaaccagag taggcaggtc agaaagtgat aataggtatt ccatgttttg gaggccgctt
    33661 tctaagctgt aatctcctgt taaaaaatag gctggattga cagggatgct cgcttttttt
    33721 agacttttca ataccccttt ttcccgttct tttgtcgaac gaaaattttt ctttccgcca
    33781 atataagcga tcttcgtatg tccgcatcca ataccgtaat ctactgcctg cataacaccg
    33841 gccgcatcat cggcagtgac gttgataacc gctggatcat tgatgtgacg attgacaaca
    33901 acgaaaggaa attctttttc tttgagatag tcgatgaaag gctgatcatc atcactttga
    33961 ctcataacga tcagtccatc gtagcgttgc aggttgattt ctgttaaatc aacaacatcg
    34021 atactttcaa cagataaact gtaatttgtg tctaatactg aatggatgcc tgcaatggaa
    34081 tctgctagga aatttgatga agttccttta tgcaaacttg agaaaaatag tccgattaga
    34141 tatttttttt gattcactaa accttttgca ctatagtttg gcgaataatt catttcctta
    34201 gctagttgtc gtattttttg ttttgtttca ttttttatta aactgctgtt attcaaagca
    34261 cgagaaactg tcgtatgaga aacgccagct gctttagcga tatctttgat cgttaccatt
    34321 ccatcatttt ccttactttt tcactttttg ttaacgcatt catcatatta cgataactac
    34381 gataagtcaa tagatctttg atatgaaaag attgcacacg ttaaaaatta cttggatttt
    34441 tatttttaat tatgctattc ttgaaagcgg ggaatgctca tttaattaga tgagtatcaa
    34501 ttttacagaa aaatgaaagc cttttatttt aaaaggagga gaggaagaaa atgaagaaaa
    34561 gtacagtatt ttataaaata tttttaccga tgtttttatt aggagccgta cttgtcatag
    34621 gatttagttt attcatttac cagaatacct atgaatcgat cgaatcgagt tatttaacag
    34681 acaaaaagaa tttgctcagg caagttaaaa ctaacgttga atggaaaatt agaacgattg
    34741 aatattcgtt ttctacgtat ggttcgacca aaaatttctc ggacatattc aagcatccgc
    34801 tgacatatac agattactcc acttattctg aaatcaggaa agaactaaat tttatagaaa
    34861 cgattgttat ggatgataat aattatgatt tagtcagttt ggctggtaaa tggggagtaa
    34921 tcaatggatc gttgagtcaa ctgaccgaag aggaagttga aaagtatcaa cagaagtaca
    34981 taaataacaa aaataattta ttttggcaaa agaaaaacaa aggaatcgaa atggtgattt
    35041 ctttgccaat gtttgaaaaa gaaaaatatg cattgggaat tgctacgata gagaaacata
    35101 caatagaaca agttgttggt gaaagtggag aagacatact ttctattgaa aacgaagttg
    35161 gcaacctttt tactactcag acagtaaaaa ataaagagca accaactgta ttaacggacg
    35221 tggatctaga ttacaatcag ctgaaagtga taagagaaaa agataaaacg tatatagtac
    35281 tgaaatcaga ttataataat tggatttaca gattagaagt tgactctagt gcaattggcg
    35341 caatgatccg caatctgaga atcgggctag ttgcagtgtc tttaacactg gtaggactca
    35401 ttggtcttct ttcctactta ttttcggaca gatttgcccg tccgatttct caaatacagg
    35461 aacgtctgaa tcttaaatca aaaggattca gtgggaaaga actagatttg gtcgctcaat
    35521 cagtcagtcg gatcatagga gaaaaagaag cgttaagtgc acacttagtt acccaaaagc
    35581 cacagctgga aacgctattt gtacttagtt tatttagaaa tcgagtagaa aggcgagaac
    35641 tagatcagcg gttgcagcaa tttggttata gttctcaaaa tgactgctat tatacagctt
    35701 tggttcaaat cgatctgtta gaagatagtc acggtgggga gcgtgatttg ctacttttag
    35761 cgatcaacaa catgatcacg gaaattgtgc caaaggaaaa tcgaatgctg ccgatcgttt
    35821 tgaatgaaga aatgcaagcc actatttatc gaatggataa agatgatgca gaagcaagca
    35881 aaaaagtcat ggagtattgc cgactcattc aaaaacgtgt aaaagaatac ttgaatttaa
    35941 ctatcagtat aggcatcagt aaccggttcg aaacgctgaa tgaaagcaag caatcggttg
    36001 atcgggcaaa agaagccctg tattatcgtg taaatactgg tccaagttca attatttttt
    36061 atcaggaaat cgttcctgct gctcatgaaa aaacacttat tagatatccg atagaagaac
    36121 aaaatcgatt gtttgaagcc attcgttctg gaaatcagga agtgaagaat ttagtacatg
    36181 aattgattga tgcattgttt gcacaaaata aaaatccttt gagtcgggaa gtagtttcgg
    36241 tccgcttgat caatgaactc gttcaattag gacagttatt gggagtagat tcaaaaaact
    36301 ttgataacat gaaacaaatt tatgtaaaag cactgaacaa ttatcatccc aaagaactag
    36361 aaaaacttgt tttagaacaa ttagttctgc caatcacaga atgctctcaa gcaatcactg
    36421 atcatgaatt taaaaatttg tcggagaata ttatgcatat cgtctacaca gaatatgatc
    36481 gtgatctgtc tttagatgtg atcgctgata ggttgcatta taaccctaac tatttaagta
    36541 atgtatttaa aaaggaaaca ggagaaaaat ttggtgattt tgttcaaaat catcgtttag
    36601 aggtagcaaa aaaatggttg aaagagacta acctctccgt aaaagagatt gcagaaagat
    36661 tgcaatatcg aaatccgcaa aattttattc gtttctttaa aaagaaggaa tcgattacgc
    36721 cgggagaata tcgaaaaaaa tatttgtgaa tcaagaggct gtttttcttg tggactgatt
    36781 caagcaataa aagaagttag aataacccta gtatttgtat ttatggatag ttaaatttca
    36841 aggctaagca cgcaaaaaga actgtgacaa aagtcttgtc acagttcttt tttacgaata
    36901 aatggcgttc aagagacagg ctatcgacaa taaggcataa ttttgtgaaa tatgggaagc
    36961 tatttttcaa aatcgcttct tattgctcct gtgggttact cgtcgcaatg tttgtgagag
    37021 tttttatcat cgctcaaggc tgaacgtctc tttcacaacc tctgatacac ggtgttccag
    37081 gagtagattc ttcgaaaaat aagccgaaat ttcacaaaaa tttaagaagc aattttcgta
    37141 aatgccttct tatttcttga agctgaccac ttctgacaca actaccattt tgcgttcagt
    37201 acataatcac cctttgacag aacctacgag tgctcctttg acaaaatatt tctgaacaaa
    37261 aggataaaca atcagcatag gcactgttgc aataacgata acagccattt taactgtttg
    37321 tgctggtgga atcacatcaa cggcactact ttctgcttgc atcccacttg atacaatgac
    37381 gatttggcgt aaaagaatct gtatcggcca tttagatgaa tcgttgatat acaaaatagc
    37441 agtcatataa gtgttccagt aagtaacagc ataaaaaagc gagatagtag caatagacgg
    37501 taacgctaat ggcagcataa ttttgaaaaa tacaccaaaa tctgtgcaac catccatttt
    37561 tgctgattct tccaaactat ccggcaaggc ttggaaaaag tttctcataa tgatcatatt
    37621 cattgcatta acagccacag gcaggatcat ggcccagtaa gaattgacca gccctagaga
    37681 acgaacaact aagaacgttg gaatcatccc cccgctgaac aacatagaaa atacaacaag
    37741 aaagttgacg aaacctcgac caaacaaata ctttctggaa agaccgtagg ccatcagtga
    37801 agttgcacac atactgacga tcgttccgac acctgtaaca ccaatcgaaa cagcaagtgc
    37861 tctaaaaatg gtcggcgtag aaagaatgta gcgatacgcg tctaaagaaa aggtagtagg
    37921 aaaaaggata aactttttcg cgacgacttc ttgtgtagaa gcaaaagatg tagcgatgat
    37981 atttaaaaaa ggaacgatac agatcaaagc tagcaaaatt agaaacgtat tgttgaaaag
    38041 tgtaaaactt ctttctccta tagagacttt ctttttcata tagataggct cctcctcaaa
    38101 ataaattaaa tgtttctgtt tctatatgtt caatttatca tcggtgaaag attacgtata
    38161 taatcatttc ctatgctaag tgtcagatag aaaaaaatag aaggtttgct atgcgtagtt
    38221 tatttgtgaa aaaatacact ttatcgtttt gtatagcaca aaaatcattg ctatgtaagc
    38281 gtttttactt acggtaaaaa tgattatata cgtaattcag atagttatct atcatgaagt
    38341 cataagcagc aaacattcac aaaactaagg aggggcttat ggaaagtgta aagaaacaag
    38401 aaccatttca actgacgcta tcgaaaaaag aagaaaagct cagaagacga aaaaaaataa
    38461 ttgcaggaat caaaacgaat aaatttttat atgtgatgat tctgccggga gtcctttatt
    38521 tcttgatttt taggtatctt cctatggggg gactcgttat tgcttttcaa gattatcaac
    38581 catttttagg aataatggga agtccttggg tcggtttcaa acatttcatt cgtttgttta
    38641 cagaaccgac attctttaca ttattaagaa atacgttgat cttgttcgga ttaaatatct
    38701 tcgtgttttt tccgatgcca atcattttgt ctttactatt aaatgaagta acgaacaaac
    38761 gattgaaaaa cgggattcaa acgatcattt acatccctca cttcatgtct tgggtaatca
    38821 ttgtttcgat tacttatgta tttttgaatg ttgacggagg ggtattgaat gaattccttg
    38881 caacaatggg atttgaaaaa atcagttttc taacatcccc tgaatggaca cgcacagtat
    38941 atatcggaca agtgatctgg aaagagttgg gctggtctac gatcatctac ctttcagcga
    39001 taactgcagt cgatacacag ttgtatgaag ctgcagaaat ggatggagca ggacgcttac
    39061 gcaaaacatg gcatgttaca ttgccggcaa ttcgtccagt aatcattacc ttgctgattc
    39121 taaaaatcgg tcagacatta gacttagggt tcgagcatat gtacttgtta ttgaattcat
    39181 tgaatcgaag cgttgcagaa attttcgaca cctatattta tacagctgga ttgaagaatg
    39241 gacagttaag tttcagtaca gcaatgggat tgtttaaagg ggtcgtagga ttgtttatgg
    39301 taattttagc taataagatt gccaaaaagc taggagaaga cggcgtttac taagatagaa
    39361 agagagggaa agatatgctg cagtcattag ataaaataaa cggaaggcta aatcgtattt
    39421 tacagttggt ttatttgaat tttctgtggg gcggtttagt catcgcaggt ttgggagttt
    39481 ttactttcgg tcctgcatcg tatgcgatgg ttgcagtgat tagaaaatgg ctgcgctcaa
    39541 aagaaacgtt tcctttgacg aaaacgtact tcacatattt taaagaaaac tatcgtgaaa
    39601 ccttgctgat gagctggata tatggcgcca tcgggattgt tttacttgtg gatctttctt
    39661 tcatacacaa ttggtatctt aggatcgctt tgtatattgc tgcgttcata tatgtgctct
    39721 cagcactcta tattttccca atcatggctc attatgattg gaaaggatat tttttcaaaa
    39781 tgaaaatggc atttttattc ggattctcac atcttcatta ctcattagtg ttgatattaa
    39841 gtatggtagg gatttatctg atgatcatcc aaacaatgcc aggtgtttta acctttatcg
    39901 gtggcagtct cttcttttcc gcagtcacgt ggacagcaaa ttctatattt tgccatttgg
    39961 aatggaagaa ctcatataca gaaaaaacag aagaaaacaa aaatattatt aaaacagtga
    40021 aggagaaaat ttatgaaaaa agcaacgctc gtaaaattag gtcttagttt tgcaggaatt
    40081 ctagcacttg ccgcatgcgg cagcagttca agtaacagtt cttcagagac agaagatgga
    40141 aaagctaaaa tcacttggtt aaatattttg catcatgctt caccgcctac agacacgatt
    40201 gtgaacttga tcgaagaaaa aaccgatacg gaactgacat tcaactggat tcctgatgct
    40261 tcaaaagaag aaagattaac aactgcattg gcttctgatg aactaggaga tatcgttaca
    40321 cttcaaattg cgatgttgaa aaactcttct gtccgcaatg cgttaaaatc aggcgtattc
    40381 tgggatgtat cagaatattt aaaagactat aaaaatttaa gtcaaatttc tgaagataga
    40441 atcgaagctg cgagtatcga cggtgccttg tacggcgtac cgatacaaaa agactacgct
    40501 cgtgctggct tagtcattcg aaaagactgg ttggataatc taggactaga tgttccaact
    40561 accatggatg agctttatga agtagctaaa caatttacag aaaacgatcc tgatggaaat
    40621 ggtgtggacg acacagttgg ttttggcgat cgtgcgccta gtgatattcg ttataccagc
    40681 tttaaacttt tcacttctta ttttggagca ccaaatggct gggctgtaga cgataatggc
    40741 aaattcactc cagaatttga tacagaagag tatatgaaag ctttgaattt ttcaagagat
    40801 ctatttgaga atggctattt ggcacaagac tttgcagtta cccaaaagac tgatcagcaa
    40861 gaacaatttg cacaaggaag aactggaatc tacactggaa tggtggatat caagaatctg
    40921 aagacgatgg gtcaaggtat tcaagatgat atggaattag tgcctgtaaa caagatctca
    40981 gatggtgaag gcggcggtta tcatgtttgg tcagaaggaa atggtattgg cggcttgcta
    41041 gcatttccca aatctcaagt aaaagacgaa gcacaattga agaaattact tcaatttgtt
    41101 gatgatcttt tagaagaaga tgtgtataaa ttgatgactg gcggtatcga aggaacgcat
    41161 tatgaattga atgatgatgg ttctatcgaa tttatcgata tggatctttg gcaacaagag
    41221 gtgcaaccgt tatcaagctc tcgtcctagt gaagtgacgt tctctttcaa agatacagac
    41281 cctgaaaaag aattctctaa tcaattgatc aaagaaaatg aagaatttgc tgtaatggat
    41341 ccttctgtgc cattggattc accgacgaac aatgaagtag gatcagaaat tgaaaaaatc
    41401 gtcattgatg ctacggtgca atacatcatg ggacagcttg acgaagatgg attcaaaaaa
    41461 gctgtagaag actggaaagc acaaggaggc gatcgaatca cacaagagta cgaggaagca
    41521 tacaaagcag cggcacagta atatagacga ttgggacaaa tacttcttgg tatttgtccc
    41581 aaatcctatt ttaattttta aaggagagca aataatggaa aactggtcaa agaaattagc
    41641 aaaaagtatc atggaaagaa cacctcgctt atatgaagaa aagtggtata aaggaaaatg
    41701 gtcttatgac tatggcgtcg tacttaaagg gttccaatta ctttgggaac gaacccaaga
    41761 aaaaatttat tttgatttca taaaagacaa tatcgactat ttcgtgcaag aagatggaac
    41821 gatccgtggc tacagcgttg aagaatacaa tatcgaccat gtaaatacag gaaagctttt
    41881 ctttttattg tacaaagaaa caggcgaaga aaaatataaa aaagcagcag aactcctttc
    41941 ccagcagttg gcaaaccatc cacgcacatc cgaaggagct ttttggcata aagaaattta
    42001 tccttatcaa atttggttgg atggcttata tatgggttct cctttttatg ctgaatttat
    42061 ccagaatttt agtgaagaaa aagattacca agatgttttg agacagtttg aaatcagtta
    42121 taaacatcta ttcgatccaa aaacaaattt gctgattcat gcatgggatg aaaagaaggt
    42181 ccaaccatgg gctgatccaa aaacaggtct atcccatcat ttttggagcc gatcgattgg
    42241 ctggtatttg atggctgcag cagataccta tgaattactt catgaagaaa cagattgttc
    42301 tttgctgaaa gaaattctag aaaagacact ggctgcatta cttccatatc aagaagaaaa
    42361 cagcgggaca tggtatcaag taacaacaga aacaaaacgg aaaggaaatt atctggaagc
    42421 ttccggcagt agtatgtttg tttacgcaat ggctaaagga atacgtttgg gactgctgaa
    42481 caaagaagaa tggcttccac aagtgaagaa atcacatcaa ggattacttg atgaatttgt
    42541 tctggaaaca aaagaaggct ggctgaactt gaataaaaac tgtcaagtag cagggttagg
    42601 cggagcagat cagcgagatg gttcatatgc ttactacatc agtgaaccaa ttatttgtaa
    42661 tgatcaaaaa ggcgttggtg cattcttgca agcattgatt gaagtggagg cattataaat
    42721 atgtatgata ttttgacatt tggcgcatca attgatgaat tgaatactga agcaatacaa
    42781 caagccattg atgctgcagc aagtgacgga ggcgggaccg tagttgttcc agcaggtgaa
    42841 tttctaacag gagctttatt tttaaaaagc aatgtcgaac ttcatttaag cgcaggagcg
    42901 gttctgaaat tctctgacga tccaaaagat tatcctgttg ttcattcacg ttgggaagga
    42961 gtccatcgaa aagtttatgc ttcctgtatc tatgctcaaa atgtagagaa catctctgtc
    43021 actggtttcg gtacattaga tggaaatgga aaaaaatggt ggcatacttt tagaaatgaa
    43081 ccagataatc tcgcttatcc tcgtcctaaa ttgatgagtt tccataactg tcatcgaatt
    43141 actgtgaaag atattaaatt gatccagtca ccaagttgga cgatcaaccc gattctttgc
    43201 agtaatgcaa cgtttgataa cttgacgatc ttgaacccag cggattcacc aaatacagac
    43261 ggaattgatc cagaatcatg caaaaatgtg cgtattagta attgccatat tgacgtggga
    43321 gatgactgta ttgcaatcaa ggcagggact gaagatacgt acgaaaggat cgcttgcgaa
    43381 aatatcacga tcacaaattg cacgatggtc catggacatg gcggtgtcgt attaggaagt
    43441 gaaatgagtg gtagtattcg taatattacc atttcaaatt gtatcttcca agaaactgat
    43501 cgaggaatac gattgaaatc tagacgaggt cgaggcggga tcgttgaaga tattcgagta
    43561 tccaatatag tcatggataa tgtgatgtgt ccatttatac tgaatcttta ttatttctgt
    43621 ggtccgcgag ggaaagaacc gtatgtatgg gaaaaaaagg cttatccgat agatgaacgg
    43681 acaccagcat tcagaaggat ccatttttcg aatatcacgg caaggaatgt ccatgcttct
    43741 gcaggtttta tttatggatt agcggaacaa ttcatacaag aaatcacatt tgacaatatc
    43801 gatgtctcga tggcccaaga tgcagttccc ggtgttcctg ctatgatgac aggaatagag
    43861 gaaatgagca gacaaggatt ttatattggc tttgcgggta aggtttcctt taaccagatg
    43921 tcgattgaaa atcacgaggg accagcattt ctattagaaa atactaaaga aatcagtatt
    43981 gagaaattcc aatcaaatca atcagaaaat atggatgaac tggttgttcg taaaaacttt
    44041 gcttatgcag ttgaatgatt ttttaggtgc tccatctagc agacataccc ctagtggaaa
    44101 acgattatac cgagaaaaac aaaacggttt tatgcttgaa tactatcaat tgtacttaaa
    44161 tgaattggaa acagtaccag ccctctatgc ttatccagaa aaagaaggac cgtttccgcc
    44221 ggtcctttac ctgcattctc acggcggaga tttttcagtc gggaaaagtg aattgatcca
    44281 tggtgctcct tacttagctt ctccatcttt tgcagaagta ctgacaggaa tgggatatgc
    44341 tgtttggtcg attgatgctt ggggatttga agaacgcgga ggtatttctg aaagcgagtt
    44401 gttcaaacaa tttttattaa caggaaaaac gttatgggga atgcgtattt atgatgtgat
    44461 cagcttgatc gattatttgg agacaaggga agacacagat actcaacggc tcgcaacgat
    44521 tggtctttct atgggaggct tattaagctg gtgggtgaca gcattggatt ctcgtgtcaa
    44581 agtttgtatt gatctagcgg cacaggtgga catcgaaata cttttgctac atcgccgcct
    44641 agatcatcat gggttttatt actatgtgcc aaatctattg acagcttata caacgttagc
    44701 aatccaagaa aaaatcgctc cgcgccctcg tcttagctta gttggaaaaa acgatacgat
    44761 gtgcttggac gaaggcgtac tgaagctgag aaaagggctg gacaaaaagt atcaatcaat
    44821 gggtagtcca gaaaactttt cttctttttc tttaacaggc ggtcatatgg aaacacaaga
    44881 aatgagaaat atttggaaac aattcataag ggagcattta tgaggatgaa agacaaactg
    44941 ataattggaa agaatgctat agataccccg acggattttg aaactatcca agaaggaatc
    45001 gattatttgg caggcctgcc ggatagccat ccaaaggtct tgatagtaaa agaaggaaga
    45061 tatgatgaat atatagaaag cagattatcc aattttaaac ttgtgggagc aggaacagta
    45121 gttatctccg gtaatcgttt tgcaaaacag gagtatcttg acggtagcca gttagggact
    45181 ttccgaagtg ctactttttt cttagaagga tcgaatgtcg tattagaaaa tcttcacatc
    45241 gaaaatacag caggtccagg agaaaaggta ggacaggctg ttgctttatt caattcagga
    45301 gatcgcgtat ctttaaaaaa ctgtcgacta tctggttatc aggatacctt atgcacgggt
    45361 ccgttgcctg atttgcaaaa agatggtact cctttttgta caccggcttc tgccaatcct
    45421 gagtattgtc gtcagaccta tgaatattgc tggattgaag gaacggtgga tttcatattt
    45481 ggtggggcag atgccgtttt tcatcagtgt catatccata gcttagccag taaaaatcct
    45541 ggatacatca cagcagcatc tacgccgaaa aatcaaaaaa cagggtatcg ttttgaaagc
    45601 tgtaccttga ctgcagaacc tgatactaga aacgtctatt taggaagacc gtggcgtccg
    45661 tatgcaaatg tcttatttga agcgtgtaag ataggtgggc atgttcaccc aactggttgg
    45721 gataattggg atgacaagaa aaatgaacag actgtcaaat tctctgaacg taataacaat
    45781 tatgaaacat cgatcaaaag acctgaatgg atacatttag aaaagcaggg ggttcagaaa
    45841 tgaaaagaaa atgggaacta ttgctaggaa tggtcggtgg cagtctatct ttgattttct
    45901 ttggcgggtt agcagttact ttaagtaata tgtctgcgtc agagtttaaa aaaagttatc
    45961 agtcgcttgc tgtagatcat tcaactttgt ctttagaaaa tacatttgga ttattacagg
    46021 acatgactgg tctgtttgcc gttgtgttgt ttatttcact tgctttttta gcagtagcat
    46081 tgtttttgac tgctaaagga aaatacttaa caactgccac tggtttgtat tttataacag
    46141 gattcatatt gttgattggc actcagttta ttgcgtttcc ttttgcattt ttttatttcg
    46201 cagctggggc attcagttta tatcgagtaa gaatgagaaa aggagcctga atcatgcaga
    46261 ctatcaaagt gaaaaatcct attttaagag gattcaatcc agatcctaat atattaagag
    46321 tgaaagagcg gtattacatt attgtctcct cttttgaatg gcttccagga atccgagtat
    46381 atgagtcgaa aaatttagtc aactggtcac atacaacgga tattttgacc actcaagtat
    46441 ctttgcaagg aaatcctaca aatggaagta tatgggcgcc acaaatcagt tatgctgatg
    46501 gattgtttta tcttgtttac acagatgtca aaagtgcagg ccgtccgttt aaagatgtcc
    46561 acaattattt gatcactgca tctgagatca caggtccttg gtccgaacca atttatttaa
    46621 atagtaacgg ctttgatcct tctttgttcc atgatcttga aacgggaaaa aaatggcttg
    46681 tcaatgaact atgggattat cgtctggaca caccgaataa atccagcggt atcgtattac
    46741 aagaatatga tgcagagaag aagaagcttg atggtcccat ctataaaata tttgcaggta
    46801 cagaattagc gaaaacggag gcaccacatt tgtattttcg taacgggtat tattatttga
    46861 tgacggcaga aggcggtaca ggaagaggac attcagttac agtttgccga gcaaagaata
    46921 ttactggtcc ttacgagctt gatcctggtt ttcctatgct gacagctagt gacaaacctg
    46981 cttctacatt gcaatgtaca ggacatggga gcttagtaca aaccccgtca ggaagatggt
    47041 acatgacata tctttgtacc agaccattaa acggtgcggc tattttggga agagaaaccg
    47101 ctattcaaga agtgtattgg acgtccgacg attggttaag acttaaagat agccagacaa
    47161 ctccgatgga agaaataatg attgagacaa atgaagaggt acaacaagaa aaagatcatc
    47221 agtttatgga tacatttgaa ggagagctaa agaagaactg gaatgccaga cgcattttgc
    47281 caaatgctga ttggtgtgat acaaaatgta gaccaggttt tttacgtctg atttccggtg
    47341 aatctcttca atctaccttc gaacatcatt tgttagctat tcggcaaacg gattttactt
    47401 ttgatgcgga gacagcattt gaatacatgc ctaataattt taatcagatg gctggactgg
    47461 tactgtactt aaatgaatcc aattatattt attgttatgt tacttggaat gaggacaagg
    47521 gaaaatgcct acgtatgata gaatcagaaa acggagtagc acaaatagaa gattggacga
    47581 ttccactcac agaaaagaag acttttttga aagtcactgt aaagaaagag acagcacagt
    47641 ttcatttgaa agacgtatca actgaagaat ggaagaaagt aggtccgcaa aaaaacatgt
    47701 gtatactctc aggagggttt actggaaatt ttatcgggat agcagtgcat gatctaaata
    47761 aaaaaagagg aagttatgct gattttgagt ttttcaagta tgatggaaaa gatcatctgt
    47821 aatttaattc tttaataaat gaggttcact aacttatttt cactagttag tgaatttttt
    47881 tgtttgtata tattgttatg taaaaaaagt tatcaatctt atgcatcaat gtttttagat
    47941 tattttttgt tttttatctg tcaagataat gcaataaaaa gccaacattg tgaatataaa
    48001 aacaagaaaa aaagagttat aattttgaaa acgtttacta gaagaggtag gaggaattca
    48061 aatgctttca caaaatgctg ttacagcaaa tacaataaaa aaagaaaaaa aggaatttgg
    48121 gatttccgat aagataggct acatgttcgg tgatctaggt aactgtttta ttttaggttt
    48181 agtcaatagt ttcttgatga tttattatac gaatgttctt ggcgtttcag gggcaatcgt
    48241 aggtacgtta ttcttgattt cgagaatttt ggatgcgttt gctgatgtga tggttggaag
    48301 attatgtgat gtctcagagt taacagaaac tggccgattt attccttgga tcagaaaaat
    48361 gaagtatcca ttttgcttag tgactgtcat tttgtttttg ccattcgtcc aacatcttcc
    48421 aatgacggtg aagattgctt atatttttgt ttcatacatt gtttatggga tttttctctc
    48481 aacaatcaat atcccgtatg gagcaatggc agcagcaatt agctcaaatc ctaatgaccg
    48541 cacctcgttg tccacgttcc gaagcattgg ttctgcgatt ggcggatcaa ctacaggatt
    48601 cttcattccg attttaatgt atacccataa caagagtgga caacaagtcg tttcagggaa
    48661 tcatttcttt tggatttcaa tcggatgtgc tgcaatagca tttgtcgcat atatgttaac
    48721 ttgtaaattg actactgaaa gagtaagagt tgtcaaaaac gaaaaagtat ctgcttctca
    48781 gttggtaaaa ggtttgttag ctaatcgagc actgattatt ttagtcattg ttgacatttt
    48841 cattgtgatc aatcagattt tagctgggac gaatatgact tatttattca atgattactt
    48901 ccataataaa caagcaatgt ctgttgctct tttatttact tacggaacag tagtagtttt
    48961 agcaccattt gctacaaagc tgacaaaatt atttggtaaa aaggaagcaa gcgtatttgc
    49021 gttacttgct tcttcatgta tgtacttgct gatgtatttc cttcatataa caaatgcatg
    49081 ggtatatctg attcttttat ttatcgctac tttaggatct ggtttattca atttgatggt
    49141 ctgggcattc attactgacg tgattgatta tcatcaatat aagacaggct taagagaaga
    49201 tggaacagta tatggagtaa attcttttgc gagaaaagta ggacaagcat gtgcaggagc
    49261 ggtaggcgga tttatgctgg cactcattgg ttaccaatcg tctacaacag gtggagccat
    49321 ccaatcagtt ttagtacaag aaaggattta tacgatggca aacttgttac cagcattctg
    49381 cttatttgta tccgcaatca ttttgctagt aggttaccca ctgaatcgtt caaaaactgt
    49441 atttattggt gaagaattga aaaaaatcaa tgaaagatag ggagaaacgt attgcggaca
    49501 aaaaaaggga ttatcaatgc tttgaacttt aaaacaaacg acactcaaca agatgataca
    49561 ataatactgc aaaaagcaat cgatcaaggt gcgaaagaac acttacctgt atttattcca
    49621 aaaggcattt atttagtcgg agcccttttt ttaaaagata agagtcatct tatctttgaa
    49681 gaaggtgcag tcctcaaggg gagaacagat atcgaagcat ttccagaaat cgatacacga
    49741 gtagccggcg tagaaatgaa atggccagca gcgattttga atgttctctc ggcgaaagat
    49801 attcttatag aaggaaaagg aataatcgac ggtcaaggag accattggtg ggagctgtat
    49861 tggggaaaag atcaaaaaag cgggacaaga gctgaatacg atcaaaaggg acttcgttgg
    49921 attgcggatt atgctattaa acgtccacgc gcttgtttac tgtatcatgc agaacatgtt
    49981 gttgttcgtg atttgatttt tcagaaatca ggtttttgga acttgcaaat cacttattct
    50041 aacgatgtat tagtagaaaa agtaatcatc cgcaacaatg atggaccaag tacagatggg
    50101 attgatattg attcttcaac aaatgtgcgt gtctatgaat gtgatttggc ctgtggtgat
    50161 gattgtattg ctattaagtc tggcagagat ggcaatggtg caagagtcaa tcgaaaatca
    50221 tcaagaatcg aggttgctag atgtaaaatc cgttcaggat acggtgtaac tattggtagc
    50281 gaagttagtg ctggagttag tgacgtatat attcacgata tcgatttttt tcagtcagac
    50341 tgtggtattc gaatgaaatc ttcaaaagaa agagggggag tgatcgaaaa tatcagggta
    50401 gaaaatctga atatgattga tgtacaattt cctttttctt ggataatgga ctggcataat
    50461 gaatacaata gaaaaaatag caatgacctt gctcaaatgc cagaaagttg gcaagcagtg
    50521 gctgaagaaa tacctgaatc aaagcaaatg acgaaagtca aagacataca catcaaaaac
    50581 gtacaggcat gcctttctcc aggttactca cttccttccc gagcttttga tttagtagca
    50641 tttcctgaaa agcctataga agatgtctgc ttttctcatt gccaaataac tgcaaaagaa
    50701 ttcgggcgaa tcgaagcagt acaaaactta tgtttcaaaa gctgtgattt atctatagaa
    50761 actggcaaca cagctgcaaa caatactttt gacaatcgtt gaaattataa taaggtcaag
    50821 cttaaataat attttagctt gacctttatt ttttttaatg atatattaaa caacgttgtc
    50881 gcgcttcatt gaaacatttt atgaaaattc caaaaaagag ttgacacatg tttttagacg
    50941 tgataagata actaagtcgt taaaaagaac cacttgcgaa gcttgattct atcgaaaaaa
    51001 acttcaaaaa agttcttgac aaataacaaa cgataagtta tgataaatga gttgctgaaa
    51061 gatttcgaaa aacgaaatcg aaaaaaactt ttaaaaaaga gttgacaatc gtttgtctct
    51121 ctgatatgat ataaaagttg ctacggcagc caaaaatgaa tcgggaaaca ctctcgatga
    51181 agtagacctt tgaaaactga acaaagtaag acaaaccaaa tgtgtagggc gtcttgattc
    51241 aattcaagac aa
//
LOCUS       DAYYGS010000010        46839 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000010 DAYYGS010000000
VERSION     DAYYGS010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46839
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.010"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(117..644)
                     /locus_tag="ACHA44_000541"
     CDS             complement(117..644)
                     /locus_tag="ACHA44_000541"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8903862.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(711..2213)
                     /gene="eat(A)"
                     /locus_tag="ACHA44_000542"
     CDS             complement(711..2213)
                     /gene="eat(A)"
                     /locus_tag="ACHA44_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8903863.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(2471..3592)
                     /locus_tag="ACHA44_000543"
     CDS             complement(2471..3592)
                     /locus_tag="ACHA44_000543"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8903864.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3718..4659
                     /locus_tag="ACHA44_000544"
     CDS             3718..4659
                     /locus_tag="ACHA44_000544"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8903865.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(4809..5705)
                     /locus_tag="ACHA44_000545"
     CDS             complement(4809..5705)
                     /locus_tag="ACHA44_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8903866.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            5974..7314
                     /locus_tag="ACHA44_000546"
     CDS             5974..7314
                     /locus_tag="ACHA44_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8903867.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            7532..7975
                     /locus_tag="ACHA44_000547"
     CDS             7532..7975
                     /locus_tag="ACHA44_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8903868.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(8479..9897)
                     /gene="nhaC"
                     /locus_tag="ACHA44_000548"
     CDS             complement(8479..9897)
                     /gene="nhaC"
                     /locus_tag="ACHA44_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8903869.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(10300..11523)
                     /locus_tag="ACHA44_000549"
     CDS             complement(10300..11523)
                     /locus_tag="ACHA44_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8903870.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(11554..12393)
                     /locus_tag="ACHA44_000550"
     CDS             complement(11554..12393)
                     /locus_tag="ACHA44_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8903871.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(12410..13090)
                     /locus_tag="ACHA44_000551"
     CDS             complement(12410..13090)
                     /locus_tag="ACHA44_000551"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8903872.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(13092..14165)
                     /locus_tag="ACHA44_000552"
     CDS             complement(13092..14165)
                     /locus_tag="ACHA44_000552"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8903873.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(14221..14459)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(14502..14735)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(14812..15483)
                     /locus_tag="ACHA44_000553"
     CDS             complement(14812..15483)
                     /locus_tag="ACHA44_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8903874.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(15570..18218)
                     /gene="mgtA"
                     /locus_tag="ACHA44_000554"
     CDS             complement(15570..18218)
                     /gene="mgtA"
                     /locus_tag="ACHA44_000554"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFM8903875.1"
                     /translation="MEKKKDTQKIYAAFAGKTKQELFELFHASANGLTEEQIERSREN
                     YGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPGDKDLFGVAII
                     FTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEEIVCGDLVRLS
                     AGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSYENLVFMGSNV
                     ISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKFMSLMAPAVIV
                     INGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGTVIKNLNAIQN
                     FGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQTGLKNLLDVAI
                     IDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGAIEEMLNISKF
                     IDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFSVKDESDMVLI
                     GYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLEINELVTGEKI
                     SEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGINDAPAMKVADI
                     GISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKATASSNFGNMFS
                     VLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKWNASSIGEFMV
                     YFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAGWFVESLWTQT
                     LVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLMPLPGNYWLWL
                     FATVIAYLALVTAVKKIYIRRFQELL"
     regulatory      complement(18560..18734)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            18907..19344
                     /gene="ndk"
                     /locus_tag="ACHA44_000555"
     CDS             18907..19344
                     /gene="ndk"
                     /locus_tag="ACHA44_000555"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFM8903876.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(19366..20232)
                     /locus_tag="ACHA44_000556"
     CDS             complement(19366..20232)
                     /locus_tag="ACHA44_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFM8903877.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(20392..22485)
                     /locus_tag="ACHA44_000557"
     CDS             complement(20392..22485)
                     /locus_tag="ACHA44_000557"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8903878.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(23234..23857)
                     /locus_tag="ACHA44_000558"
     CDS             complement(23234..23857)
                     /locus_tag="ACHA44_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903879.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(24016..25413)
                     /locus_tag="ACHA44_000559"
     CDS             complement(24016..25413)
                     /locus_tag="ACHA44_000559"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8903880.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(25445..26416)
                     /locus_tag="ACHA44_000560"
     CDS             complement(25445..26416)
                     /locus_tag="ACHA44_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8903881.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            26790..27215
                     /locus_tag="ACHA44_000561"
     CDS             26790..27215
                     /locus_tag="ACHA44_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903882.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(27703..28404)
                     /locus_tag="ACHA44_000562"
     CDS             complement(27703..28404)
                     /locus_tag="ACHA44_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8903883.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(28420..28860)
                     /locus_tag="ACHA44_000563"
     CDS             complement(28420..28860)
                     /locus_tag="ACHA44_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8903884.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(28860..29435)
                     /locus_tag="ACHA44_000564"
     CDS             complement(28860..29435)
                     /locus_tag="ACHA44_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFM8903885.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            29676..29885
                     /locus_tag="ACHA44_000565"
     CDS             29676..29885
                     /locus_tag="ACHA44_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit beta"
                     /protein_id="HFM8903886.1"
                     /translation="MYYFKQQILVSFINEKIILFSCYVSLINDVSLNKKADELNDQNL
                     IIFVHQLLLKIFLLTINSWICKNIT"
     gene            complement(30283..30795)
                     /locus_tag="ACHA44_000566"
                     /pseudo
     CDS             complement(30283..30795)
                     /locus_tag="ACHA44_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014566402.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(30921..31622)
                     /locus_tag="ACHA44_000567"
     CDS             complement(30921..31622)
                     /locus_tag="ACHA44_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8903887.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(31730..33173)
                     /locus_tag="ACHA44_000568"
                     /pseudo
     CDS             complement(31730..33173)
                     /locus_tag="ACHA44_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738852.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(33187..35052)
                     /locus_tag="ACHA44_000569"
     CDS             complement(33187..35052)
                     /locus_tag="ACHA44_000569"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8903888.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(35173..36023)
                     /gene="licT"
                     /locus_tag="ACHA44_000570"
                     /pseudo
     CDS             complement(35173..36023)
                     /gene="licT"
                     /locus_tag="ACHA44_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
     gene            complement(36333..37046)
                     /locus_tag="ACHA44_000571"
     CDS             complement(36333..37046)
                     /locus_tag="ACHA44_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HFM8903889.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(37114..37440)
                     /locus_tag="ACHA44_000572"
     CDS             complement(37114..37440)
                     /locus_tag="ACHA44_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HFM8903890.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(37563..37790)
                     /locus_tag="ACHA44_000573"
     CDS             complement(37563..37790)
                     /locus_tag="ACHA44_000573"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8903891.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(38112..39365)
                     /locus_tag="ACHA44_000574"
     CDS             complement(38112..39365)
                     /locus_tag="ACHA44_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8903892.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            39426..40166
                     /locus_tag="ACHA44_000575"
     CDS             39426..40166
                     /locus_tag="ACHA44_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8903893.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(40252..41010)
                     /locus_tag="ACHA44_000576"
     CDS             complement(40252..41010)
                     /locus_tag="ACHA44_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8903894.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(41007..41471)
                     /locus_tag="ACHA44_000577"
                     /pseudo
     CDS             complement(41007..41471)
                     /locus_tag="ACHA44_000577"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
     gene            complement(41490..41987)
                     /locus_tag="ACHA44_000578"
     CDS             complement(41490..41987)
                     /locus_tag="ACHA44_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HFM8903895.1"
                     /translation="MILLLGFILWQTPVHASEFNFAVDFELPANQIDKNKTYINVRME
                     PGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAK
                     TVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQV
                     KAILI"
     gene            complement(42055..45222)
                     /locus_tag="ACHA44_000579"
     CDS             complement(42055..45222)
                     /locus_tag="ACHA44_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFM8903896.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(45446..46330)
                     /locus_tag="ACHA44_000580"
     CDS             complement(45446..46330)
                     /locus_tag="ACHA44_000580"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8903897.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSITESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(46425..>46839)
                     /locus_tag="ACHA44_000581"
     CDS             complement(46425..>46839)
                     /locus_tag="ACHA44_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903898.1"
                     /translation="LFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSVLVLGTLII
                     AGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKI
                     FTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        13397 a         9846 c         8011 g        15585 t
ORIGIN      
        1 tttcatttct aaaggttgag tttatattat caaaagaatt gtcataacct aaaaaaggca
       61 ctgctaagca aaagcagtgc cttgaaatat tttatttaaa taacacaaaa acataattac
      121 tcataagaaa ccaattctgt attatctacc atgaatgtaa agaacccctc attaggcatc
      181 ggatgaaaat aagcattgat cactcttatc aaggcattat ggcaaactaa cagatagaca
      241 ttttcttcgt cttcgatgca ttctttcaat aaaggaacga tccgtgcata cacatctaag
      301 actgactccc catttgggaa tcgtacagcg aacgctaaac gtgctttttg aaagttctca
      361 tctttgcttg gcagtccatc ataatcccca aaatccattt ctaccaatcg ttcatcaacg
      421 gtcagcggca agctgagacg atcagcaact gcttgtgctg tatctcttgc tcgttgaaga
      481 ggggaatgga tgattttcgt gattggaaca ggcaaatcta ctaccttttc tgctagtttc
      541 tgtgcctgca tctgtccttt ttcagtcagc ggcacatctg cccgtccgca aactaatcct
      601 tgtgcgttcc aagttgtttc tccgtgtctc gttacataaa gcatgtgatt ctctccttcg
      661 ttggcaaagt tccccttaac cataccttgt tgttcttgag attacaaggg ttagattctc
      721 tcaagagaaa taatctcagt agatacttta tccaggaagg tttgatcatg ttccactagt
      781 aacatggcag gtttcacgtt caagatcagt tgttctaatt gttcttgatt gaagacatcc
      841 aaataattca atggttcatc ccatgtatat agttcagctg gctgtgataa agatttagcc
      901 aattccactt ttttccgttg gcccatactc atctgctcga tcttgttatg aaaaacatct
      961 ctttccatcc caagctttcg gaggttgttc aaaaatgctt ggtagtctac ttggtttttc
     1021 tctgcaaatt ccgccaacgt tcctcgattg tcttcataat tttggcttgc ataactaatg
     1081 ctcagatgtt ttggctggta tttttctcct ataaccttgc cgttgaatgc ccccagaaga
     1141 taatggataa tggatgactt tcctgcaccg ttaggacctg aaatcgccac ccgctgatga
     1201 ggctcgattg taaaatgaat tggctcgaat aacagatttt cataccctaa ttgaagatct
     1261 tctacgctta aaagtcgctt atggtgagac gcttggctat tcatcgtcaa agaatcgata
     1321 tattcgatat cttttaatag tttttctttc tcgctgatct gggtctccat ccgattcacg
     1381 attgctttgg atcgtttcat cgtccgtgca gcatcagcac ccactgctcc tttattcact
     1441 cgtctagatt cagtatcgat gaatccgact tgtttctttg ttttatctcc ttctcgggaa
     1501 cgagaccatt cggctttttc agctgctgtt ttctttagcc tactgacttc ttttttcaat
     1561 ttttcatttt gagccatttc aaattcatca cgaagttttt tctgttcttc atagatagag
     1621 aaattccctt gataaagttc cagttgactt ttttcgattg ctaaaacatg gtccactact
     1681 tcatcgataa atccccggtc atggctgacc acgatgaagc cttgtttttt ctttttcaaa
     1741 taagccgcta cttgttttct accagagata tccaaatgat tcgttggttc atcgattaac
     1801 gggaaatgag tgtcatccac aaataaaagt gccagtagga cttttgtctt ctctcccccc
     1861 gatagtgtgc tgaattctct ccataagatt tcaggatctg tttgcatcaa ttggagctct
     1921 ctttcgattt cccatatctc aaaatccgta atatcattta acacgtaata ggttaaacgt
     1981 tctttatctt ttgtctgttg cgggaaatag gcaaattctt gctgatggat tacttgccct
     2041 tgataaggta gtttgttttg tagaatattc agtaaagttg tctttcctcg accgtttcgt
     2101 ccgataagtc ctagtttcca ttgtgtgtca aaatttagat ttgcttgttc aaataataat
     2161 gtgccttggc tgtcgtagcc gaatgtcaga ttttttattt cgattttaga cattttccat
     2221 cacctcgtaa aaaaagacca gtcctatttt cagaactggc taaagaggat gcaagggttg
     2281 gatacacaca aaaagaccca tacatcgaaa aaaccaagcc aatcccgaaa atctgcacac
     2341 tgcttccttg tctccacaag gtacagtctg aatgcagctt tcatcaatcg acttaatttt
     2401 tcaatgcacg gatccctcac tttgtcctat atttgaaatt acgatagcat aaagaaaatg
     2461 attttgtcaa ctacttcggg aggatggtga aaaatctgct aagtgtctgt cctaagattt
     2521 ttgttttttc taatgtttgc ctgtgttcca ttgcttcttc taaacctatt ggacccaaat
     2581 gaatacttaa gacaattggg agaaaatcag aaagtccttc tagctcttgg tctctacttc
     2641 cgcaaatagc gattattggt tttcgatatt tttttgcaag cactgctaca ccgtatggaa
     2701 cttttccgtg gatcgtttgc tgatcgatcc gcccttctcc tgtaatgatg acatctgctt
     2761 gttcgatctt tttttccaat ccagtgattt ggctgactaa atcgaaacca gaaaccatct
     2821 tcgctcccaa caaaaaaagg gctccaccaa taccgcctgc tgctccactt cctggaatat
     2881 catctaaatc cacctcccag aaatctttta tttgctgagc aatctttcct gcttgtttat
     2941 ctaagaaagc tagcgtcgta tctattcctc ctttttgtct tccaaattgc caagcagctc
     3001 cttgttgacc tgtatatgga ttggtaacat ctgcagcgat aatcaatttt actttagaaa
     3061 caagaacgtt tcttcgatca aaatgaagtt ttttagtaga taataaagga tttccttctg
     3121 tgtacccttc taaagaagca cccaaggatt ggagtaatcc tagaccacca tcgctacacc
     3181 cgcttcctcc taaagtgaca ataatttgtt ctacttcttg aagcagtgca tctttaataa
     3241 gttctcctaa ggcataactg gaagcaagtc ggatagtttg atccgttggt ttatcaagta
     3301 gatctaaccc caatgcttgt gcagattcga tgactgcggt gttttttcct tgccaagttg
     3361 tcaataaata gctgattttt cgctgacgga ataagagatc catcccatca atggtacgcc
     3421 atgttcccct gagcgcgtaa tagattgctt ctgcagtatt ttctccgcca tctgcaatcg
     3481 gcacattgat ttttcttcct ttacgatctt cccagccatt tagaactgcc tgatttagtt
     3541 caaatgaaga agcacttcct ttaaatgaat caattgccgc caaaatatcc atctgtatcc
     3601 ctcactttct tcttaagtaa gacacttttt ttcatttttt tcaaacaaaa caatgctatt
     3661 ttcttccatt tttctatata ctaaaaggga actcattgaa taaaggagag aaaaaacatg
     3721 aaaatcgcaa tagctggggc cggagcaatg ggcagccgaa tcggattgat gctacatcaa
     3781 agcggaaatg aggtcctctt gatcgatcgt tggcctgctc acattgaagc cattcgtaca
     3841 aatgggttga ttgctgactt taacggtaaa gaagtggtcg caaaactgcc aatctattca
     3901 ccagaagaaa tcatcgaaag caacgaacac gtggacttga tcgtagcatt aacaaaagcc
     3961 aatcaattag atgatatgtt ttgttcgatc caatccatta ttactgacaa tacttatgtt
     4021 ttgtgtttac taaatggatt aggtcatgaa gatgtactgg aaaaatatgt gccaaagaaa
     4081 aatatcttat ttgggatcac gatgtggaca gctggtctag ctggtcccgg aaaagtcaca
     4141 ttgttaggtg atggagaaat cgaactagag aacttggaac cagaaggcga agcatttaca
     4201 aaaaaagttg tagaagtctt tcaagaagca aatttaaatc cgatctatag ccataacgtt
     4261 cgttattcca tttggcgaaa agcttgtgta aatggaacat tgaatggttt atgcacgatt
     4321 ttggattgta atattgctga actaggcgcg caaaaagcgg cagagagcat ggttcgaaca
     4381 atcgtatctg aatttgcttc tatcgcagca aaagaaggaa tcatactaga tcaagaagaa
     4441 gtgtaccaac atattgcttc tacttatgat cctgacaaca tcgggttgca ttacccatct
     4501 atgtaccaag acctgatcaa aaaccatcgt ttgactgaaa tcgactacat caatggtgcc
     4561 atttggcgaa aaggacaaaa atatgatatt gctacaccat attgtgcctt tttgacccaa
     4621 ttagtccatg caaaagaagg aatccttggc gcagaataaa aggctctctg atgagaatca
     4681 gctgaatcag cagctggttc tcttttttta agcccaataa aagatcaaaa agaaaacaca
     4741 actctcatga tgccataaga attgtgcttg tttgtttcag tacataatct cttcaaaatg
     4801 taggtttttt atttgttttc aacagaggag aggtcatatt cgtattgctc ataaatagtg
     4861 gaatcgttag tgataaatcg ttttgcccaa ttttctgcat cataaaattc caatagctct
     4921 gataggattg gcttaaatgg tgtatgcaga aattccggta attcatctaa atcacgttct
     4981 cgaagcaaaa aaatattttc aggacgataa aaatgataat gagcaatcgc ttgattatcc
     5041 gtcactagtt tcttttgaaa atacatggat tcaaatgtcc gcaaagtcac tccttgctga
     5101 ccctcccgca acagttccag aatcccgcgc gattcccctg tttttttcag gtactcttca
     5161 taagtaagat atccaggttg ttcattccct cgatgtggaa gaatgaaata gttggtcgta
     5221 acatctcttt ttataaattc ctgcttatag ctttctgctc gttcttttct cccattattc
     5281 gttgcaccaa aaaatagatc cgtctttggt tcgctagaag aaaaattcat atgtttcgaa
     5341 taaaatgaac tattatgttt cagcccaagt tcttttgctt ccaaaaaatc aaaatgataa
     5401 atctcatcga ttgccggttc atcttgtgcc tgcttcagca tcgcctcgtg ttctgctttg
     5461 aagtgattcc aaaaaaataa cacaattttt gcattggtaa atgggcgcac gcgtcgagcc
     5521 atctccatat caaatccata atctaaaaaa acgatcagct gataatttgc caactgttgt
     5581 ctagctgatt taatgaattc cgactgtagt aaatcttttc cggaccaaac atctaaagag
     5641 cggatggaag gcaaaaaata aagtgattta ttttttgcaa ctagcagtac gtcatttttg
     5701 ttcatctttt ccgcctcact ttctctgtca ctttttccta tgatagcctg tttaaatagt
     5761 aaaaagccag taaaacgttt cctatatttg tatgaaaatt taacatttgt atgaaaatat
     5821 gatgaaataa atcattcttc tattaaaatt cgcctaaaag ttttctgaat agttgtaatt
     5881 gtctgaaaat ttgttatcat tagtcattgt taaatattgc tatttattct taatataaat
     5941 tttgattgtg aatttaaaag aaagaggtca tttatggaag ataaaaaatt gaaaaaacaa
     6001 cttagctcca gacatatcac catgttagct ttaggtggag caatcggtgc aggtctattt
     6061 aaaggaagtg gcgaagcgat tgggatcgcc ggcccttctg tattgattgc ttttttgatt
     6121 ggtgggctga ttttatatat cgtgatgaaa ggtctgggaa aaatcgtttt aagcggtggc
     6181 gacacgcatc acggattatc tggtttgata cgcccttatc taggtgctca ttctgctgat
     6241 tttactgatt gggtctactg gtctatgtgg attatcaata tcatcgctga agctgttgca
     6301 gctgcttcct ttttgcagtt gtggttcccg cacattccag cctgggtatt tgtttttatg
     6361 ttggctgttt taactacgat catcaatctt tattcggtac ggttattcgc agaaacagaa
     6421 tattggctgg cttttgctaa aatcagtgtc attatcttac tgatcatttt tgctacttat
     6481 cttgtcggtc agcaaatgtt gggcacaggc gttttcccta cccttcagca attgactgac
     6541 catggtgggt ttactcctca tggtatgaag ggaatcatca gttctttgct tgtagttatc
     6601 tattcttacg gcggttctga actgattgct atcacggtaa gcgaagcaga tgatcctaaa
     6661 cgtgcgattc ctaaagcaat caaaggtgtc atgggaagaa tcatttcctt ttacatcatt
     6721 cctttgtttt tgcttttgat tctttttcct tggaatactt tagcaggcac gaatgtcagt
     6781 ccttttgtga tggtatttga aaaaatgaat atcccatttg cagcggatat cgtcaatttt
     6841 gtgattgtcc ttgctttgtt ctcatctatc aattcgggtg tgtatgcatc ttcaagaatt
     6901 ttgttcttcc gtttgaagga tcgaaaaggt tcctctagaa aactagctgt tttaaacaag
     6961 catcaagtac cgcaacgtgc tgttttattt tgtaccagtg ttctttatct tggtgtagct
     7021 ctttcttatt ttgtcggtga caaattattt ggctatctgg caggttccct ttcgtataca
     7081 gtattattga tctggatcat tatcagtgcg gcatcctttg tactggcact aagaaaagga
     7141 acattatggg ataaaggaat cagctttttt gcattagctg tcttaggatt gattttcctt
     7201 ggtatcctgt tcacgaactc cattggggta actgtattga ctggcctgct ttacctgttc
     7261 atttatttca gttatcaaaa aaagaatgat gcgttcgtat tgacgaatga atgaaatcga
     7321 tagaaaattt tattttattt atgtggaata gatggagcta ttgaagaaag gagttgtgac
     7381 aaaagtcttg ttgcaactcc tttttccgaa taaacagtct catgacctct tggtaaacgg
     7441 tgttcaaaaa ccctctgtgc aaaataattg attgttctcc atgctcatga catgatagaa
     7501 gaaaggaatg aaaaaaagga gtttgtttta aatgaaaaag tattggaagt ttttgttgat
     7561 gatcggtgtt tcaacggtag ttatgtttgg tatgatgtac ttgaatgtct atgcgctcga
     7621 tcatatcttc ttcagtcgta cacgagtatt catggcgttg atgatgggtg cagtcatggc
     7681 agtcatcatg ctgttgttca tgtggaaaat gtatgaaaac aaaaagataa atgttatgat
     7741 cttagccgct agtcttcttg tttttgccgg ttccttgttt atggtacgaa gccaaactac
     7801 tgtcaacgac acagcttgga tgaaggccat gattcctcat cattctatcg ctatcctggc
     7861 aagtaaacga gctgatttaa aagatcctga agtaaaaaaa ttagcggaag aaatcattca
     7921 ggcacaagaa gaagagatcg acaaaatgaa aaaaatgatc aaagaaatgg aatgaccatt
     7981 atccgtccaa ataaaaaaag aggatgttcc agaagttatc agcttcaaga aataagaaga
     8041 agctacttct ggaacgccgt gaataagggg ttgtgaaaaa gctgccctgc atcaagtaat
     8101 aagaacagcc aaacaaaaat agcttctcct atttttcgtt tggttaggct tattaccgca
     8161 gatgcaagct tttgaacacc gtttattaag aggttgtaaa atcagcgttc tgacctgagt
     8221 attagagaga caaatggaaa aatagttcct catattttgc cattttttga gctaataccg
     8281 agggtcagct gattgaacac cgtttatcga gaggttgtga aaaagtcgct tagtttcgcg
     8341 tattaagaaa aattttgtga aatggctttc cacatttttg tataaatttg gcttaatacc
     8401 gagaaactgg cttttgaata ccgtttattc gtaaaaggga ttgtgacaaa agtcttgtca
     8461 caatcccttt ttatgttttt attgttctaa ttgcgctgtc ttgcttactg cttcttgcgg
     8521 tttcacaaat ttttttgaga ttccccaacc tgtgaaggca ctgaccatcg aaaacacagg
     8581 agaaagcaaa ctgaaaaata cgaatggcaa gtaagtgagt gtaggaatac caaatgtact
     8641 tgctacgaaa cttccggcaa ttccccaagg cactaagtag ttgattactg ttccaccatc
     8701 ttctagcgtt ctacctaata cagttggatc aaatcctgct ttttcataag tttctttaaa
     8761 tgcattacca ggcagaataa cagataggaa ttgttcacca ataaaaatat ttactccgat
     8821 acttgtcatc aatgttgtaa aaatcaaatt tggtgtgttc gtcatctttc ttgatactcg
     8881 ttccatcacg gttgagatca agccaaattc gatcaacaat ccacctaatg acaaagttag
     8941 aataatcaat gacacagtag gcatcatact ttcgattcct ccgcggctaa gcagtgaatc
     9001 gatttgttgg tttccagtat ttgaaacaaa ccctgattcg atgaccccag ccaacttcgt
     9061 taaggaagtt tgtgggtttt gaataaaaat cattcctact gctacagcga tattcaacaa
     9121 aatcgttaaa attgccggca cctttttcca tgcacacagt aacattaaaa ataaaggaat
     9181 cagtgaccag attgaaatag taaagtttgc ttctagaaca tcagtcactt cacgaataga
     9241 tgaaagactt gcggctttac ttgtgtgtcc taaaatcatg aataaaaaga aagaaacaac
     9301 aaatgccggg acagttgacc acatcatatg cttgatgtgt ttgaacaaat cggcatcaac
     9361 aatagctgct gctagatttg ttgattcgga taatggagac atcttatctc caaaaacggc
     9421 acctgaaaca atagctccaa caaccagcgc tggatttaag cctagtgttg tcccaatacc
     9481 gaaaaatgca atcccaatcg ttgacataac agtaaaggca ctccctacag cgctacctac
     9541 gattgcacac acaacaaata cagaagggac aaaccactgg acactgatca atttgaaccc
     9601 aatgaccatt agagttggaa tgatccctgc ttgtatccat acagcaatca atgaaccgac
     9661 taaaataaaa ataaagattg ggatgattcc tggtttgatg ccattgatga ttccttcgtg
     9721 cagccaatct gtcgggtatc tttttatcac tgcatacaac atgaccattc cgatcgctgc
     9781 taagatcgtg atattaggtg agacctttaa tccgacaacg ccaatcgcaa tgcctaaaac
     9841 aataaccatt aatacaagtg ttgcttctaa aaaacttggt gttccttttt ttcccatgat
     9901 aatctctcct tttttagttg ttcttatgtt ctaaaaaagt taagatatcg gattactccg
     9961 ccgcaattca tccgcgattc ctcctttata aaaataaaaa gacctgtttt aaactaagga
    10021 cgaatgactc cgcggtacca ccttaattaa aaacagatct gtttttcact ttcaaacttt
    10081 tattcaaatg tatggacttt ccatgtaatt cgtcaattag gcttgatcgg ttcacagcga
    10141 ccaccgactt tctgaacaaa acacctaacc gctactgcgt gaaaagttga cttgtacgaa
    10201 gtttaccttc cataagatca tttgtcaatg aaaaagaaaa tattttttca tttttctgaa
    10261 aattattttt ataactttca cacacaagtt tattttttac tagaaagtag cgccctcttg
    10321 tttttttctt gctactattt cttgatgttc ttgcttgatt ctttctaata gctttggctc
    10381 tttcaacaga tcaagagctg tattggcgag tagttctgct gcaatcgcaa tagaagcaag
    10441 gcctttgtca cttttagcag cagctttgaa ttcttctgtg tgtccggcaa tatatgtctc
    10501 agagattgct accgtaggct ggattgttgg tatgacttgg ctgacatttc ctacatcaga
    10561 agaaccgaga cttgttcttt gttccgtttc tatttcttca tctggaacac ctgcttcttt
    10621 tgcatgctgg aagaataagt catcaaaaga aggagtaacg atgacatcat caactgcatt
    10681 ttggaacaat ccaaactcaa aagtcgtgcc cgtacttatc gcagcacctc gtacgatatt
    10741 ttccactttt tcatatactt tatctaatgt ttgccgattt cctgcacgta aatagaaacg
    10801 tccagcagcg taatctggaa tgacgttggc agctactcct ccgtctgtaa tgatcccatg
    10861 gatattgaca tcttttggca attgcaagcg tagagcgttg attccattaa acacttggat
    10921 caatgcttcc aaagcattta ctcctttttc aggtgctgct gctgcatggg aagcgacacc
    10981 gtaaaatttg atgtcgactg gatcattcgc taaagattct gtcgttttcc cataacgaaa
    11041 agctggatgg acgcataacg ctacatcgac atcctcaaaa aaaccttctc gaacaaagct
    11101 gcctttagct gatccgttct cgcccccttc ttcccctggg gtgccatata cacggatttc
    11161 tccaccagtt tcatcaatga attgcttcac tatgctggcg gctaatacgg agtaagtacc
    11221 aaacaaatta tgcccgcatg catgaccaat acctggtaaa gcatcgaatt ctgccaaaaa
    11281 ggcaattgtg ggaccgggtt tattggaaat ataacgtgca tcaaaggctg ttcggtgtcc
    11341 agctacttct ttttctaccc aaaacctttc tttttctaat tgtttaatca acgctgctga
    11401 ggaataaaac tcatagttac ttacttcagg atgttcgtgg atatctagag caagtgcttg
    11461 atattctggt aaacgatatt ggattagatc cttcaattgg tttgttgaga ttgttgttgt
    11521 cataaaaaat tcctcctatg ctagtcattt ttcttatttt ctcccgaatg tttcccatgc
    11581 aggcacgctt gatccttttg atgttttctc gattactttg actgtgtctt ctgtttgata
    11641 agcgtctact attttttgat acgttttatt gttttcatct tcttttcttg caacgatgat
    11701 attcacataa ggacgtgcat ggtcatccac tggttctaag aagatggcat cagaagcagg
    11761 attaaatcca gcatctacag ctacaccgct attgatcact gaaatgtcta catctgacaa
    11821 agcacgtgct gtttgggaag catctaattc agaaatgtct agattttttc tattctcggt
    11881 aatatcagaa acagtgggcg cttgtccttt tgccttatcc actttgatca atccagcagt
    11941 ttgcaataac agaagagctc tgccgccatt tgtaacatca ttagggattg ctacttttgc
    12001 tccgtctttg atatcagcta catccttcaa agtgttagaa taaattccca gcggtgcatt
    12061 gacagtattt ccgatcgata ctaaatcagt tccgtgttct ttattgtaat tgtctaaaaa
    12121 gaattgatgt tggaaagaat tcaaatcaat cgatccatct gctaaagcca catttggttg
    12181 attgtaatct gtaaatttca cgtattccaa attaatgttc tcctttttca aacggtcttt
    12241 cacgttgtcc catacatctg tatcttctcc aataatccct agttttacga tagtttcttt
    12301 tttgtcagtt gtatctgctt ttccttggct acaccctgcc aaccaaataa ttgctacaaa
    12361 tgtaatgact acaaaacgaa taattttttt cattttttct cctccacttt caatgtgtaa
    12421 tttttttaat cagttgatta ctgataaatt gacttaaaaa aactaagatt agaatcaata
    12481 atgtagccac aatcgttaca tctgtttgga atcgattata ccctctcgat atagcaagat
    12541 tccctaatcc gcctgcacct actgctccag ccatggtagt cagtccgatc acgttgatga
    12601 tagtcaacgc cgagacccga atgataccag gcaaaccttc taacaaatac acacgtaaaa
    12661 tgataccgat tggactggtt cccatcgctt ctgccgcttc gatcacccca ggatcaactt
    12721 ctagcagtgc gttctcgatt tgacgagcaa aaaacggaac aactccaaaa atcaatggca
    12781 ctaaagctgc tttcgcacca atcgtcgttc caactaagaa acgagtagtc aatgctaaaa
    12841 gagaaagcaa gataatgaaa gggatggaac gaatgatatt gatcagctta tcaagtattt
    12901 caaatagcag acggttagct agtaatccat tagggcgtgt cactactaac gtcacaccta
    12961 agatgattcc aatcatcccg ccaatgaatg ctgtccaaaa agacatatat agtgtttgct
    13021 tcgtcgcttc gacaaattca tccggaatca aataaacatt gggcaaatat gttttaaaga
    13081 aatcagtcat tttatcttcc tccttcgatc actttgatac gactatctaa atcgtttttt
    13141 tgtaaaatat ttatttttac accagatttg atcaaataat ccaatgcttt tttccgctgt
    13201 ttggtttctc cagctaaagt aacgatcaaa ctccctaacg gcgtttcttg taataactct
    13261 acattgccat ataaaatatt cgctgttact tgatatttgc tgtacagatg agcgattaga
    13321 ggttcgttcg tctgatttcc aatataactt aattccacca gccatttatt ggcgatctga
    13381 tcggcaaaag cagaatgggc aatgatcgtt tctaatgctt ggtcaagatg agtagctgtt
    13441 cgaataaaat ctttggtcaa ttcttcttca ggattgctga agatctggat gctgctacct
    13501 ttttcaacga ttcttccatc ttccataacc gctaccttgt tgcagatttc tttgacaact
    13561 tgcatctcat gagtgatcag gacgattgtt aaacctaact ggcgattcaa tttttttaac
    13621 aatgcaagga tctgaagggt cgttttcgga tcaagtgcac tggttgcttc atcacataat
    13681 aataccttag gttcattcgc caaagctcgt gcgattgcta ctcgttgttt ttgtcccccc
    13741 gacagttgtt taggataagc agatgctttt tcttccaaac cgacaagttc cagtagttca
    13801 ttcacttttt gacgacgttc ctgtttcgat tttccagaat actttaaaga aaaatcaacg
    13861 ttgtcaaaaa tagttcggct ttccattaga ttgaaatgct gaaagatcat accgatggcc
    13921 tttctttctt ttcgcaattc tttgggagag agctgactca gattcgtctg attgacaatg
    13981 acttctcctg ctgttggttt ctgcagcaaa ttcatcacac gaactaacgt acttttccct
    14041 gcacctgagt aaccgacgat tccataaaca tcgccttttt caatcgttaa atctacagaa
    14101 tctactgcct gtatctcttt ttctttttgt ttgaaacata cagaaacatc tcgcaattca
    14161 atcattaaaa ttcctcctta tctaaaaaaa gcgtcctcaa gaaaatgaaa atgtcatttt
    14221 cttgaggacg cttttttgcg tgttaccacc tcatgttcaa gctgtgttca cacacaactc
    14281 ctcttcaagt acgatcatac tctagcaaga tatcggtcgc agccgtcatc gccttacatc
    14341 tatgctcggg atgaattgct cgatggccat tttccaatag tcgttccttg cttcttctca
    14401 gctaccgaag ctctctgtca aaggttcttc tatcgtactt accatttcaa tgcaaataat
    14461 attcaaaaaa aaagtccttt tactaacata aactatgtca gtaaaaggac gacgtatcgc
    14521 ggtaccacct tttttcggaa gtttccttcc ctcaagccca acatctcaaa aagagagatt
    14581 ggcgttcgtt ttaacggtcg aacagttccg aatttgccta cttatcgaca aattattctc
    14641 caaggccatt ttcagctttt cttacttgac tcttctcacc agccagagcc tctctgcaaa
    14701 gtttccaaac ttactttcct tttcaacgaa atcgatattt tgttgttggg attaatctac
    14761 atgaggaaaa ctgaaaagtc aagtacgaaa ttcaaattat tttttctata tttatttttc
    14821 tgttttttta tttaacattt caataacagt tcgaagatct tttccttccg caagaagttt
    14881 caatttttcc ttttcttcga cggtataatt tcggacaaaa ctggaatcag gtatcaaaac
    14941 aacgcctaat cctgctcgtg aagcagctgt tgctcctact aaagaatctt ccactgcaaa
    15001 ggctgaacta gcttctaatc ctgttcgttc caaagctgtc agataaggaa tcggtgaagg
    15061 cttgttctcc gacacttcgt ctccgaatgt aaacgtgtcc aagtaatcag ctaatttaaa
    15121 gtgatccaag atatctgttg ctcttttcgt aacagtggtc gtagcaatgc ctagtttcat
    15181 atgacgttct tttaagtctg ccaatatttc ttttgcatat ggttttaagt ggatggctcc
    15241 gcttgtcagc tgttgataaa aatattcttc acgcttcgct cggacttcct gtactttttc
    15301 agggctacct gctgctttca ctaaaatatc aaaactttgt ttccagtttt ttccgcccca
    15361 agcgtcaacg atttcttttg ggatatggat atcaaattgc tgaaatcccc aaagccagcc
    15421 atcacgatac gttttttctg tgtcaactaa caaaccatct aaatcaaata ttgcaccttt
    15481 catctataca ttaatccttt ccaaaacaat acacatagtt tagcacgact ggtccaaaaa
    15541 gaacagaagc aaagaaccag ccttctcgtt tataagagtt cttggaatcg acggatatag
    15601 attttcttca cagctgtcac aagcgccaga taagcgatga ctgtagcaaa taaccataac
    15661 cagtaattgc caggaagtgg catcaagcct aattcagcac caaaacttgt aaaaggaagg
    15721 acagaaccga caataattcc taatgtagtg ataacaaaca tggcaaatgt tgcgttgctt
    15781 tggataaatg gtactttcgg tgtacgcagt gcatggagca ctaaggtttg tgtccatagt
    15841 gattcgacaa accatcctgc atggaataat gcgatgaaaa tcactcgctg tgaactatcc
    15901 aatgtatgga aacttccgcc cactacagca ggacaaataa caaaatacat caacaagtaa
    15961 gtagtaatat caaatatcga acttgttgga ccaaagtaaa ccatgaattc cccaatacta
    16021 gatgcattcc atttttttgg ctcgtccaaa tattctttat ccatccgatc ccatggcaag
    16081 gaaatgcagg aaacatcata gatcagattc aaaaacaaaa gttgtaaagg cagcattggt
    16141 aaaaatggca ggaacgtact tgcaatcagc acggaaaaca tattgccaaa attagaactt
    16201 gctgtagcct tgatatattt catgatattc ccaaaagttt ctcgtccaga aagaaggcct
    16261 ctttccaaaa tcgtcaaatc tttttctaaa aggatcacgt ctgcagattc tttcgcaata
    16321 tccacagctg tatctacaga aataccgata tcagccactt tcattgcagg agcgtcattg
    16381 ataccatctc ctaaaaatcc gacagtatga ccatttttac gtaaagctgt cgtcaaacgt
    16441 gctttttgct gaggattcaa ttttacgaat acttcatggt cttcagctac ttgtgctaat
    16501 tcatgatcac tcatttccga gattttctca ccggtaacca attcattgat ctctagacct
    16561 acttgacggc acactgaacg tgtaactaat tcgttgtccc cagtcagaac ttttacagct
    16621 acaccatgtt ctttcagagc tttcaacgct tgtttcgttg tttcttttgg tggatcaagg
    16681 aaagcaagat aaccaatcaa aaccatgtct gactcatctt tgactgaaaa ttctccaacg
    16741 acgcttggat tggtcttttg tgcgacacca atcactcgta agccatcttc attaagtgac
    16801 tgtacttttg caagtacggc ttcttttcgt tcttttgtta atggtgtaac agtaccgcca
    16861 agatcaataa atttagaaat gttcagcatt tcttcaatcg cgccttttgt gatcatttgt
    16921 gttttaccag ctggatcagc aaccacaaca ctcatacgac gacgttcaaa atcaaaagga
    16981 atttcatcca ctttttgata attgatctta tctgtatcta aagtttctgt tgcttcatca
    17041 atgatagcca catctaacaa atttttcagc cctgtttgat agtagctatt tagatacgca
    17101 tggcgcaaaa cacgatcatc ttcatttcct tcacaatcca tatgatattc aagaatgatt
    17161 ttatcttgtg tcaatgtccc cgttttatcc gtacataaca catcgattgc accaaagttc
    17221 tggatggcat tcaaattttt gatcactgtt ccttttttgg ccatcacact tgctcctttg
    17281 accaaattcg tcgtaacgat cattggaagc atttcaggtg ttagaccaac tgctacagat
    17341 agtccaaaaa gaaaagcttc tagccaatcc ccttttgtaa gcccattgat gacgataact
    17401 gctggagcca tcaatgacat aaatttgatc agtagcatgg atgttttatg gatgcctaat
    17461 tcaaagcttg attgaattgg tttttcagat agcgtttctg ctacatgacc aaatagtgtt
    17521 tcatttccaa cagaaacaat gattccttcc gcacttccac tgatgacatt acttcccata
    17581 aagacaagat tttcgtaact tgtttcgctt tgctgatcgt cttcacatac tttcgcatgc
    17641 ttttctactg gatagctttc ccctgtcaat gctgcttggg aaatgaataa atcctttgcg
    17701 gacaataaac gcatatccgc tggtatcata tcccctgctg aaagccgaac aagatctcca
    17761 cagacgattt cttccatagg aatctctgtg tactttccat tacgttttac agctgccgtt
    17821 actttcacaa gtgatttcag tttttcagcc gcttgattgg aacgtacgga ctgaatcaac
    17881 gtcattgtcc cgctgacgat aaccatagtg aagataatgg ctacaccgaa aagatcttta
    17941 tctccaggag ctgctaatac atattccgtg atgaatgaaa taattcctaa tgcgatcaac
    18001 actaatgtaa atggcgtgat gtatgctttc aatatttcta caatgaatgg tgtttttttg
    18061 ccatatgaaa tcgcattttc accgtaattt tctcttgaac gttcgatttg ttcttctgtc
    18121 aatccatttg ctgatgcatg gaatagttca aataattctt gtttcgtttt tccagcaaaa
    18181 gctgcataga ttttttgtgt atctttcttt ttttccatct caatctctcc tttgtgtcca
    18241 aaagacagct gttctcgctg cttttggtct ttagctattc atcggatcga tagagaaaaa
    18301 actgaaaggg gctgagaaca gatagataat agccctggcc tttcctctag atccttgttt
    18361 ttcgaatgac tgtcatccat ttgttctcat ctcctttact aagtaatttt aattcatgac
    18421 ttccaatcat ttttcgaata gttatttttc caattagacc acctcctttt tatttttcac
    18481 cgatggggat ttgcggcaac aaaaaaacct ctcgccaaat actcagcgaa aggtttgaaa
    18541 aagcatatta aaaaaagctt ctcagatctc atcgttgagt tttgacactg tacaacgtaa
    18601 aaagcagata cctgctttta gctaccttat gaaaagcctt atctcgacaa ttcctgttct
    18661 acccattggc gtctctcgac gtttttgggc agtagcctat gtttgtatag gagcctcacc
    18721 taacaaatct tattcggttt tccttcgtta atataacaac taaaaaagag gatgtcaaat
    18781 caattttcca tgtttttcat acttttttta aaatacacac gttatattcc tgtctcacgc
    18841 ggttttattt gtctcatttt ccacctcccg ctataataga aatatagtag gaaacgaggt
    18901 gctgttatgg aaagaacact tgttattatc aagccagacg gcgtgagacg ccatttagtc
    18961 ggaagcatca tccaacgttt tgaagcaaag ggactggcca ttgctgaaat gaagtttgaa
    19021 actatgactc ctgaattagc aaaagaacat tatcagcact tgactgagcg cagttttttt
    19081 gatgaactga tcgattacat gacttccggt cctgtcgttt atcttgtgct ggtcggcgag
    19141 gaagtgatcg atatcgtccg taagatggtt ggagcaacaa aagctgccga tgctgttcca
    19201 ggtacgatcc gaggagacta cgcgctgccg gggactgaaa acatcattca cgcttctgat
    19261 tcccgtgatg ctgcggtaaa agagatcgcc cgctttttcc cgccattcga tataaacaaa
    19321 ccgtctgaaa aagcggcgaa gtgatacaaa ttctaaactg ctggtctagt tgctgggttc
    19381 agcaatggcc agcagttttt tctctaagga cctccgttga ttctttccta acatatattc
    19441 gagcactgtg aagaattctt ggtagtcttt cgtcttttct tcccctaaag cagcagaaat
    19501 caatctttga taataatctg ccatcaatgt ttcatagatc actgtacttc tttctttatg
    19561 tgataagctc cgtttcatat cttcattcac actacgtgcc tttttcaacc gaccattttc
    19621 tatataaaac gaagtcaggt tcattaaaaa agtaaagaga tactgctcat gattgttcaa
    19681 ctgtgctaat cgtcgcattc tttttttgat tttcaaataa ttgttctcaa tgaaccgatc
    19741 actgaatatc caaggaaaat cagctagaat agatagttca aattgaccat agtctggtat
    19801 ctgcatcaag tattcttcaa tgattcggca ttcataacgt aaaaattcaa attctgtctc
    19861 agatacttct gttttttcga ccatgtgaag attcaaaacc aaataataca gatcttctgt
    19921 tttcttaaaa tgcctacggt tattgctgat ctctttataa ggatcaaagc tgtcttcttg
    19981 tacccgatct agcattcgtc tttttttctt taactttatt ggttcatcca gctggtttcg
    20041 catccattca tattctgcaa gtgtcatgtt cattcgctct aaaaatgatc tcaatgttac
    20101 aaatgacatt tttttcactg aatttgtctc aatcccgact agagaagtct taaaacagat
    20161 tccttccgct aattgggact gtgtcaactt cttgttgact cgaagtttct tgatcaattc
    20221 accatcatac ataatagttc tcctttttaa tttaaatttt tccacattca ataaatagat
    20281 acgttaaaaa ggaaaaatac acgaaaaaaa taaaaaaatg tttttttcat aaaaaatacc
    20341 aaccttcaag cattagaatg ttgtctaact actgaaggtt ggtttgattg tttattgatt
    20401 atttttttgc tgttcaacgt ctgttcttgt tactggatat aagttggttg ttgttttatt
    20461 accatttttt gaaaagacac ttgtcgaatg ttcgcctttt tgcttttcga ttgcttcatt
    20521 acgttccgct tcatttttat agtcatattt tttcggatca atcgtctcaa atccgtttgg
    20581 cacataaaac cgcaagagat tttcctgatt cagcttatct gaaagactta acgcagtctt
    20641 gactttttcc tgatctttgg agatttcctc atcgattcct gctgctttcg tatcgatgat
    20701 ttctcctgtc tgattattgt aagcttttcc tcctaggacg gtgtatttgg gtgtcacaaa
    20761 gttcccattt cgaaaagcaa ctacttgatc atgctgagaa gataataagt ctgtaccaaa
    20821 atgaagataa tctttatcat cgattcccaa tagatgtagt aatgtcggca aaacatcgat
    20881 ttctccaccg tattcatgat tgactgtccc tttagtataa ccaggcatat ggatcatcaa
    20941 tggcactcgt tgcatctgtg cgttgtcaaa ttcatcccat gtatctggat ctttaccaag
    21001 agcgcttgct aaatctttat tatctgtatt cgagatccca aaatgatccc cgtaaatcac
    21061 aaagattgaa ttttggtaaa taccagattt tttcaaatac gtaaaaaatt gttccaatga
    21121 ttgatccaga taatgtgccg ttcgaacata attatctact gtgctattgc cagtattcaa
    21181 agatggaaag tcgaaattct tgtcatccgt ataataaggc gtgtggttgg ttacggaaag
    21241 aaatttcgtg taaaacggct gttgtagatg ctcaagatat tgggcagatt ctctgaataa
    21301 atctttatct aatatcccat aacctaacgt ttcgtcggat tcatcaaaat aagatcgatc
    21361 aaagaaattg tcataaccta agtttttata tacatgatct ctattccaaa aactagctac
    21421 attcccatgg aaaacagcgc ttgtatagcc ttgcttttgt ccaaggatcg ctggtgcggc
    21481 ttgaaagaca ttgtctgacc caagttctgt gaataatgag ccttgaggta aaccaaatgt
    21541 gccagtttcc aacatatttt cagcatcact tgtctttccc tgtcctactt catgaaagaa
    21601 attgtcaaag ctgatcgttg cttgattctg gaaaatacta tttaaaaacg gtgtgacttc
    21661 ttgtccgtct accttcatat taatcaaaaa ctgctggaaa ctttctaaat ggagaacgat
    21721 cacatttttc cctttagcga tcccaaatgt ttcgggattt ggctctgcat aatgtttctt
    21781 tgtataatct aaaacttcat caatcccatt actgcttgca tgcgcgcgaa cactgctagt
    21841 cattcctgtt ttgattccgt cataaatagt atacgcatcg attcctagat atttgacaat
    21901 atatgaacgg tcaaatgttc gctgcagcaa ttgtggacgg ttggcttcgc tcagcgctag
    21961 atttcctgaa aaaataagcc ctgaaagaca ggtaacagcg atagccatcg gtttgccgac
    22021 agggttgctt ttgataggta ttttcttgac cttcaaccaa accagtagcc caatgaacac
    22081 agcgatatcc agccagtaga tgacatcatg gggcttcatc aaagaaaagg agcttcctga
    22141 caatccttgc gacactttcg aaaagcctaa tacagatttg actgtaatga aatctgtgaa
    22201 ttcccgataa aaaataatat tcaagtacaa caaaagcgta tttaaaatat caatgatcaa
    22261 aagaatagct aacgctggct ttggcttttt aatatacaaa gaaaggccaa aaaacaataa
    22321 ggtagtcgca attgggttga tccacaagat aaagtactgg atcgtacctt ccacacctaa
    22381 tgaaaaatct gcatagtagg caatgatcgt cttcagccag aaaaagaaaa cagctgtcaa
    22441 aacgattgct aaacgatttt ggcacaattt tttaagttgt gtcacgtata tcacttctct
    22501 ctattcacat tttttcttat tttgctcctg aaaaatgaaa atttcctaaa aaagttgtta
    22561 tcttaagatt tttttattct ttctaaagat tattccgtcc acttttcaca gcataaaacc
    22621 gctgcgatgt aagcgattaa actaatgaag agataaaagt cttgtggtga ccgcttcact
    22681 ccacggtatt caaaagccag tttctcggta ttcgtacaaa aaaagaggct gtgacaaaat
    22741 tcttgtcgca gctccttatt tcttgatgca ggacagcttt ttcacaactt cttccttaaa
    22801 cggtgttctc ctgccaactc ctcggccaca agtcacaata tttataaaac atgagaagct
    22861 gttttttgaa attgttcctt gtggctcaga gttaaacggc aggctcaaga cctcttgata
    22921 cacggtgttc aatcagctga ccctcggtat tagattatgt caggacgcta attttacaac
    22981 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    23041 atataaggag ctattttttg aaattgctcc ttgtggctca gagttaaacg gcaggctcaa
    23101 gacctcttga tacacggtgt tcaatcagct gaccttcggt attagctcaa aaaacggcaa
    23161 aatatgagga actattttgc cgtttgtttc tctaatactc aggtcaaaac gctgatttca
    23221 caacctctgt ttttcattgt ccataggatt gatattgtcc ttgtgatgac tcattcgtaa
    23281 taattgaggc ttgattttct tcacttcccg ttatcgtttg tccactgcca gaatctgaag
    23341 gacttgttcc tgtcgactcg tttttttgag aagagacagt tgttttcttg acttgttcat
    23401 ttgtcttttt ccattccgct aaacgatcgg cgaccacttt tttgtctact ttttcagaat
    23461 aagcggtgat cacttttcca tttttcaaac gggctacttt gtcgccatca tgagtcaaaa
    23521 gcaaaacggt caaatgcttg tctttaaatt gatattgtct gagagaaatc gcactaagta
    23581 cgtcatcgat attaataaat tgattttctt cttttccttt tggcaaataa atgtccaatt
    23641 cgcctttttc gtaattggca gttattttat actccgccca ccaataagtt gagaattctt
    23701 ttttgaaatt atctactgat tctttgcttg ctacgatttt ttctgtttct ttctgagcaa
    23761 gatctgctgt catttttcct gaaaatgctg cggataaaat aagcagtaag aaaaaaccga
    23821 ataaccaaaa gagaagaaac ggtctttttt tcctcatgct ttcctccact cctatttaaa
    23881 tttgactatt ctattatata caaaaaatga aatagaaaga caaaaaagtt gaaaaagatc
    23941 gtaaataatt gaacttcttt ttcacaaaat ggtaagaatg gagaatttca cgcatttgct
    24001 ctcaactatt ctttcttaag cctcaaatcc gtttttatct gatacgtctt tgaaccaatg
    24061 acccgatttt ttgattgttc tttttccatc ttgtgctaag tctactgaaa taaagccata
    24121 tcgattctta tatgcattgg tccacgacca gttatccata catgtccaca tatgatagcc
    24181 taaacaattg gcaccttctt gaatggcttg atggacatat ttcagatgag attggacaaa
    24241 ttcgatccga taatcatcgt ggatcatgcc atcttctttg ataaatcgtg cttcgttttc
    24301 tacacccata ccgttttcag aaataaagca gtcgatattt ccataatgat ctcgtacatt
    24361 gatcaaaata tcatagattc cttgttcata gatctcccat ccgcgataag gattcatttt
    24421 acgaccaggc atttcataat tgtcgaaata atcctcaggc attggaccgt ttttagtatc
    24481 gattggtgtt tcttttgctt ttacccggcg tggctggtaa taattgatac ctaaaagatc
    24541 tactgtattt tctttgataa ccgtcaaatc ctctgcatgg atcacaggca tgagatcaag
    24601 ttcttttact atctcaatca attcacttgg aaactcgcct tttacagctg gatcaaggaa
    24661 tgaacgattg aagaacgcat ctgctatttg cgctgcttta acatcttcag ggtttgattc
    24721 gtctcttgga tagcttggtg tcagattcaa gatgatacca ataacaccac cttgctccat
    24781 ttcatggtaa gatttgatgg ctaacgctga agccaacgct tcatgatagc caacttggat
    24841 cgcttttttc atatctgact cttctggata atgccatcga tataaataac ctgcttcgac
    24901 tggaacgatt ggttcattat gagtaaacca tttttttact ttcgaaccaa acaattcaaa
    24961 acaacttttg gcatattttt cataggcttc aaccgtttct cggttcaacc atccgccttt
    25021 ttcttgcaga tacatcggca tatcgaaatg atacagattc acaaagggtt cgataccgtt
    25081 ttcaatcaac tcatcaaaat aacgatgata gaaacgaacg gcttcttcgt tgattgttcc
    25141 agattcatca ggtatcaaac ggctccattg gatagatgtt cgaaatgaat tatgcccagt
    25201 ctgtttcatc aactgtacat cctcttgata cttcgtataa acttgcgagg ttttttcagg
    25261 tcctacttga tcaaagaact tttgcggttc ttttttgtac caataatcaa aaatgttctc
    25321 tccttttcca tcgcctgaaa tccttccttc tgtttgtggg ccacttgctg cagaacccca
    25381 ccaaaaattt gctggaaatt gatatttttt catcaaagca cctctttttc ttttttagtt
    25441 cgttttagtt gattccatgt atgatatttt ccatcacaat cactttttca ttttcttcct
    25501 cttctttttt gattcgtttt aacagctgtt ctgctgctcg ttccccgatt ttatctgttg
    25561 gtggcgtgat cgtgattttc ggaaggatac tcagatcgtt tttatctaaa taatcatagt
    25621 gactgaacgc aatatccttc ccgattctga caccttcttg attgaaacat tcgatggcac
    25681 caatcgacat attgtaattg ctgacaaaaa tagcagttgc ccctttttct aataatgctt
    25741 ttgccccgta atagccgctt tttctagaat agtctcctgt aaagacctct aattggatcg
    25801 atggatcatt ttggaccgct tctttggctc ctgctaaacg ttctcttgct acgtagtctg
    25861 tttgagtggc agcaataaat ccgatttttt tatgtccttg ttcaccatga tgagcgatca
    25921 cttttgctac actttctcga tcattcacta atattgtatc aatacctgct ggtccttctg
    25981 gacaattcaa tgcgataact ggaatactca tttttgtgag catttcaaat ccagaccact
    26041 cttcttcgga taagaagaca atcagcccat cgatcacatg cttcatcagg tattcaattt
    26101 tttgttcaat tttttctggc tgatcggcaa acccgcttaa taataagcta tacccatttt
    26161 cttcagctac tctttcgatc cctgagacaa cttccgaccc aaaacggctg gaaatcccgt
    26221 ttatcaaaag tccaatagag aagcttcgat tattcttcaa ccctttagca atgatgtttt
    26281 ctttatagtc caacgctgaa atcgcttctg ctattttttt catgttcttt tccttcagtt
    26341 gctgaccatt caagtaacgg gaaactgttc ccactgaaac accagccatc ttagccacat
    26401 cacggatcga tgccatacat tctcactcct gccagttaca tttgatttct ctttcttgtt
    26461 gttcgattat gatgaccgca ttctttgcat agtgttgctt gtcatcaata tccatataac
    26521 taaagtcttc agtagagcaa cttcctctca ctattaatta tacttaaaca agtatttgaa
    26581 ccggttcatt tttaatatag cattttttta aaagattgca acactttttt aaaacgcttt
    26641 ctgaaagtgg cggatttttt gcctttttaa aattttattc taaaatttac tctatttgtg
    26701 ggtgttttgt gcgtttttct cttccatccg tttgaagttt tacattccaa tggtataatt
    26761 tctttatcct atcaaatggg ggaagtattt tgaagaaatt aatcgtacta tttattttgt
    26821 ctgcgggact tttgacattg actggctgtg atttgtttgg cggctctgac caatcctcgg
    26881 acgaaacaac cgattcttct gtcttaaaag cggaaaaagc agataccacc aacagtgtat
    26941 atacgctaaa agtacgtaaa gatatggatt tatccgcggg acagttgaca tttacagata
    27001 aaaaaatgga atggacacgc acgtatcaga cacaaggaac tgattcttct gaatcatctg
    27061 aagaaaaaac agtgctgaca gatatcaaga tcactaccaa agatgacgaa tatattattt
    27121 caggaaaaga aaataaaaaa gaaacaacag tcatatttac aaaaatcggt aattaccgtt
    27181 tgaaggatga ggatggcaac atctacagtc tatagtgccg tctgttggat cttcttttgt
    27241 ttctcccctt cttttctgct atagtaaaga agcaagatga ttgtaaagga gacgaaatgg
    27301 atgatcgata gtcctgatta ttgtacatgt aataacaaca aaaatatcca tacagaattt
    27361 aatgaccaag gaagcttgag catatgcgac aactgtcaca aactagtcaa agattcattt
    27421 aaaacatttg aatcttatcc tgttcaaaat tatgtaaaaa gtagaagaag ataaacaaat
    27481 ccaagcaaaa ctcactatgg ctgattttta tgaacttcaa actcagcttc ttcctttgat
    27541 caagcttcat cagtaaaaca aacagtggat catttcagtt tacaaatcaa agagtaaaga
    27601 tttatttatt tcaaatattt gaatataaaa taaccaaacg atagaaaggc ttattctaca
    27661 tcacgtagta atttttcttt cccgtcgttt ggttttttat ttttagccta attcttgacc
    27721 ttttgcatgc tggttatccg taaaatattt tccagcaggc tttccttcca attcagctaa
    27781 tttttcttgg atctgacgga tcaccttttt tgcatcttct ggattttcta caaaatcgaa
    27841 tttatcccca tccacttgta ttttaggaca tacatcaaaa tcttcatacc attgattata
    27901 gcgggtgttc aacgtttcat aataggtata taacgtctca tcgctttcta gctgttcata
    27961 gtcacgccca cgttttttga tacgttctag catcgtgtcc aaagaaactt tgatatggac
    28021 catcaaatca ggccgttttt ttggagcaac atcatctaac tcgtttagca tcgtatctaa
    28081 taagctatcg tactgctgta cttcgatatc cgttacccga cctaaatcag cgtttagatg
    28141 gaacaacagg ctatcttcat agatggatcg gtctagcaca ttgtcatcgt tcactaaagc
    28201 atttttcatt gctaaaaatc gcttgttcaa aaagaagatt tgcaataaaa aagcgtactg
    28261 ctcaggattt gaataaaaga gcggcaatac ttcgttatct tcgacatttt cataaaatgg
    28321 tttagaatca atctcttttg caagcatatc cgtcaaactg ctcttacctg caccaatcgt
    28381 tcctgaaatc aaaatcactg acatacaacc cttcctcttc tataagtact cgcctacgta
    28441 agtttcaaca ggcaatgttt caaaatcata ttcacgtaca gcttggtctg attttaaata
    28501 cgtatggaga ctttgtgtaa tcatcaagtt cgtaggtacc gtctcttctt gtacaatgat
    28561 catcggtttt ttcaacatag ttgccacacc taattcgtaa gccgttcctg gatcgattgt
    28621 ttgatgctca aagtcaagga tagcaacaac aaattctgca tttgtcagtt cttccatatc
    28681 tttttcgtat acttcctgcg cccatccagc gctgaatagt tcataattgc tttcttggtg
    28741 atgacgtgga gagtagaaac ttgtgactgt tttgttttcc tccaacgctt tttcgatacg
    28801 acttactcga tcgatttgct cctctgaaaa aaatggtcct gctaaatata tttgactcat
    28861 tattttgcct ccttcaataa taactgattt ttaatagata ctgctcgtaa ttcattgacc
    28921 agttccaatg gattgttgga aaagattact tgatccaacc attttttatc cataaaatcc
    28981 tcttttgcaa aagtttctaa taattgttgc aagccgctgt aataaccgcc aatattcaat
    29041 attccgattg gcttgtcggg cgtcagaccg attgctatcc aagaaagaag ttgtgagaat
    29101 tcctccaatg tacctagacc tccagggaaa acgaggacag catctccctt ttcaaataat
    29161 aatacctttc tttcatcgat cgtttctgta ggaataaatg tagtaacatc ttttccaggc
    29221 aattcagttt caaaaagccc attcggatag atacctgtga cttttccgtt attttccagt
    29281 actgttttac taatcactcc cataatcccg gagtatgaac caccgtaaac cagttcgatt
    29341 tcttcttgtg ccatgtattt tcctaacgtt tgcgcaataa atttgtaact ttctttatta
    29401 ccaaaacggg aaccgcaaaa aacagtaagt ttcataaaaa aacactccct aactcaaaag
    29461 tctatcttca ttctacacgt gtttttttaa aataaaagac tagattttgt agttttttag
    29521 tggtatatag gatagacgcc actatatata gtatttagaa aaaaatcatt tttcagccat
    29581 tttatagata aaacaaaaat aaataacgtg ttttcatcga tatgaaatga cttacttata
    29641 gaagatactg ggatttcgtc ctatatgcca ataacgtgta ttattttaaa caacaaatac
    29701 ttgtttcttt tattaacgaa aaaatcattt tgttctcgtg ttatgtttca ctgataaatg
    29761 atgtttctct aaataaaaaa gccgacgaac taaacgatca aaatttgatc atttttgttc
    29821 atcagcttct cctaaaaatc tttttgttaa cgataaatag ctggatttgt aaaaatatca
    29881 cttgaatcgt cactgctgga agaaaattca gtgataaccg cacctgttgc gcctgcctgg
    29941 aactagtgtt tgatattggg ctcgaacgtg tactgatctc tagcatgaag aataatttct
    30001 ttttttactg tataataact ttctttcctt tttggaatag tacaggaaag tttttttgtt
    30061 tcctttcctt cgacataagg tataccagac cctttcgaca acgaaaggtt ctcatttttc
    30121 cttcatcgtc atttcaagga gggtgccgat gctctggaca gtttcgatcg ctcctaaacc
    30181 aaaatctgta tattcgattc tttctaactc tgattctttc ataccattcc tgtctcaagg
    30241 tacatctatt gtactttttt cttgtaatcc attctgcctc tcctaactaa ttgtcaaatc
    30301 atcccaagat atgtcttctt cctctttttc ttcttggatg atttctgcat tcggatctac
    30361 tggttttttc aataataaaa tggttattcc tgtgacaaaa gaaccagcta acaatgcacc
    30421 aatgaatgcc catggtctgg tcatcgtagc gactgcaaac attcccgatg ccggaatacg
    30481 gctatctgcg ccccaaaaca tgcttaatcc gccacctact gtcccaccga ctcccgttgc
    30541 tatgacattg cgtgccaagt cgttcaatac gatagggaaa catccctctg tgatctgaca
    30601 gatccccata ggaaaagcgg tcttcaatgt atcgatttct tgacgattga gtatattttt
    30661 acgaatgatt ttccctaaga aataagcgat cgtcaagccg aatggggtta ccatcgaagc
    30721 taaaatcaat acagtgattg gttcatagat tccttctgaa aatcgtgcca caccccctta
    30781 tccagaataa atggtaatcc agaaacagcc gtgtatctgc ggtaataagc ctaaccaaac
    30841 gaaaaatatg agaagctatt tttgtttggc tgttcttatt acttgatgca gggcagcttt
    30901 ttcacaacct ctttttttat ttatgtttca taatcaatat ggtaaggatg cagctccgta
    30961 tggtagaccg cctccgaata ttcaatcatt tccccaattt gcgtagacga tttccgtatc
    31021 cgtttgatgc cgatctgtgc gtctgttttc agaattcctt gttcttcttc tgttaaaaat
    31081 actgccgaga aatcatcaga aaatgagtca atagtatatc cttttttcgt cagcaaacga
    31141 tacaaagatt ctttgccaaa ttcttctctg ctgaattgca tcatttcttg tggaagataa
    31201 tgaacaaaat aaatataagg ctgattgttc aaataatata agcgcttaaa acaaacagct
    31261 tgctcaccga aaaaggaaaa caaaggatct tccttcttta tatggaccaa ttgcaaatcc
    31321 agcgtttctt tccggacttc atatcgggag ttctccaaga tttgcgtaaa gcttgccgct
    31381 ttggataatc gattgtaagg acgattgctt agaaccgtcg tcccacgacc acttttcttt
    31441 gcgacatatt catctgatgc cagcagttcg attgcctttc ggacagtgat tttgctgact
    31501 tgaaacagtt cttctaattc actttctgtc ggcaacatcg aaccgactgg atattttcct
    31561 gagaaaatat cttctttaat tttatctgcg acttccaagt atagtacacg tgtccttttc
    31621 ataacgtcac cctttgcttt actattacta aaagaatagc acataaaaaa taaaaaaaga
    31681 acacaaatca catgtgagaa gtgtgttctt tttcattcag ctgctttaat tacaagttta
    31741 cgccatttga ggcaattact tctttatacc aatcaaaaga ttttttcttt gatcgattca
    31801 gtgtcccatt tccttcatcg tctagatcca catagataaa gccgtaacgt ttactcattt
    31861 ctcctgttga tgcactgaca agatcgatac atccccaagg cgtatagccc atcaaatcta
    31921 caccgtctgc tactgcatta gccatcgctt cgatatgacg acgtaaataa tcgatccgat
    31981 aatcatcaat aatcgtgcca tcctcttcca tatgatcaat ggctccaaga ccgttttcaa
    32041 caatgaataa tggtttctgg taacgattgt ataattcatt caatgcgatt ctcagccctt
    32101 ctggatcgat ctgccagccc cattcacttg cctcaagaaa cggatttttt actcctccca
    32161 gcaagttccc ttctgaagtt gctgcttgtg gatcagtctc attgattgcc gtagacatat
    32221 aataactaaa tccgatgtag tccactggat attcttttag caactggaag tctttttccg
    32281 tttgctctaa tttttccgga tcgactccat attttttatg ggtacgcgct gtgtatgcag
    32341 ggtattctcc acgtacttgg acatccgtgc agaaaaaatt gaattcttgg ttttggatca
    32401 ttgttgctgc ctgattgact ggattggcat cgatgctgta agttgttgca tagataatca
    32461 tgcagccgat ttggatatcc gaacgcaatt cgtggccgat ttttacggct ttgctacttg
    32521 caacgaattg attatgccat gcttggaaaa tattttggta ctctcctgct ccattgcttt
    32581 ttactaatcc ttgacttaaa gctggaaagt gaaaggccga attgatttca ttgaaagtca
    32641 tccaatattt gactttactg ctgtatctct ctaatactgt ctgagcaaat cgttcataga
    32701 agtcgatcag ttttcgattt ttccagccac cgtattcttt tgccaaatga agaggcattt
    32761 cgtaatgaga aatcgtgatc acaggttcaa tatcatattt taagcactca tcaatcagct
    32821 gatcataaaa ttctaatccc gcttcgttag gagtaccttc gtctccattt ggaaaaattc
    32881 gtgtccaggc aatcgaaaaa cgataacatt tgaatcccat ttccgcaaac aaggcaagat
    32941 cttctttgaa acgatcgtaa tgatcgattc ctcgatgatt cgggtaaata tatttttctt
    33001 gatcaatcgt ccagtcaaat tcttcactgc cgataatcgc aaagcgctgc ttacctccag
    33061 gcatagcatc tgcaacggat agtccttttc cgtctcgatc ataagcccct tctaattgat
    33121 ttggcagcag ttgctccacc ccataaaaat ttttctgaaa aacgtgattg catcctgatt
    33181 tcctccttat tttacaattg ccagaacatc ttcgtttgca ataatctctt tttggtctaa
    33241 ttcaagtata tcggcaaatg catcagtatt cgtaatgaca actggagtga ttacgctata
    33301 gccagctgct ctaattgctt cgatgtcaaa acgaatcaat aaatctcctg ctttgacgag
    33361 atccccttgt ttgactaaga tctcaaagcc attcccattt aattctactg tatccatccc
    33421 gatatgaatc aaaatctccg ctccgtcagt cgttgtgatc cctacagcat gtccagttgg
    33481 aaaaatagtc gtgatctctc cgtctgcagg tgcaactagt tctcctactt ctggatcaat
    33541 agcgatacct tttcccatca cgccagaaga aaaaacttgg tctggtactt cactcaatgg
    33601 cagtacttta cccgttaaag gactttttaa ggtatgccgt gctgaagtaa tcggttctcc
    33661 tgcattcgca tgtttgtttt ctaattgatt gtcttgtgtt tgttcgtcaa atcctaaaat
    33721 caatgtacct acgaatgcaa gaacaaaagc aattcctgta gctattactg ctactgtcac
    33781 atttgactct acaccatcca ccgtattaat gaaagtcgga atcgtcaata agctgaccaa
    33841 actgctggag aaagctttta ctccggaaaa tccgataata gctccaccaa tacctccaga
    33901 aatacaagcg gcaatgaatg gtttcttcaa aggtaatgtc acaccataca cagttggttc
    33961 agtgattcca aataacgaag tcactgtaga agaaatccct aatgctcgaa gtttcgtatc
    34021 ttttgttcgc aatgctacag ctaaagcagc cccaccttgt gccaagatgg ctggaagaag
    34081 cattggcatc aatacatcaa acccatattg ttcgatattc aaaaacatga tcggtacgaa
    34141 tccccaatgc atgccgaaca tcacgaatac ttgccataaa cttcccatga ttagacctgc
    34201 tacgattgga ctaaatccat aaatcgagtt gaagagaccg cctaaagcat taccggcaac
    34261 cgtcccgatt ggtcctaaag caagaaatgt taatggaacc atcactacta aaacgacaag
    34321 cggaactaaa atcatttgca gaaattgcgg aatcactttt tttacgaatg gctctaattt
    34381 actttgaacc catacggcta gaatgattgg tatgacagaa gaagtataac cagatggtga
    34441 aagaatcact gggatcccaa agaaactgat agcttctccc gcaccagcaa ctgctgtgat
    34501 agctggatac acaagtgcca tagcaattgc tactgctaaa aactggttgg ctttgaactt
    34561 tttagctgct gtaaaagcaa gcatgacagg taagaaggta aagacaccat ctgctgcagc
    34621 aaacaatatt tgataagtac cgctatcagc tgtcagccag cccatcaccg ttgccaatga
    34681 taagaatcct ttcaataccc cggcggctgc catcgcccct agaaaaggtg taaatatact
    34741 agagataata tcgatcagac gattcaaaat atttttggac tcttcttttc catcctccga
    34801 aactgcaaga ccttcctcat ccacgatcga ttgataaaca tctgctacat tactgccaat
    34861 cactacttga tactgaccgc cactttgtac gacttggatc acgtccgggt cttcttttaa
    34921 cttttgcgta tctgctaaag attcatcttt taatctgaat cgcaaccgcg tagcacaatg
    34981 tactaaactt ttgacattct tttgtcctcc taccgcttcc catacacgac gaccaactgc
    35041 ttgattatcc attgtttttt ccttctttct tttttattta aaaaataaaa acccagattg
    35101 aaaaaaacag tcacaaacat tcgtgaacct attttttcaa tccaggtttt gcctgccata
    35161 cagtaacaat ccttatgatt ggatgctttt tttctcactt actactcttt gaatatgaat
    35221 cgttaaataa atctgctctg cgatccccat ttcctgtttt tgcgtttgtc gaagatactc
    35281 attgatccgt tcgacacaat gaaacgcttt tggatacttt ccttgaacta atgcgtagag
    35341 aaattcatct tgctcgtcag atccttcccc tttgagataa cgctgggcaa aaaactgcag
    35401 atgagtaatg atacgctgat aattcaaaga ttcttcatcg aacacgatgc cgaagaattt
    35461 actgatgata ctcagtatat ctcttaccat tgtggtagcg gtcatcgtca cttgcatgtc
    35521 ttcgtttcct tgttggctat tcacaaaatg aaaggcaata aaaccggctt catcctcaga
    35581 aagttccaca cccgtctttt gtttcacgaa caaaagagcc tctttcgctg cttgatattc
    35641 tttcggataa aatttttttg tttcaaataa taatggatta ggcagttgga tattttcttt
    35701 tgctcgtgtg atcgcataat gaagatgatc ggtcaatgtc agataaatat taggcgaaaa
    35761 ttcaactttt agtttatctt cagccagttc gactacttgt ttgacccatt cgatctcctc
    35821 tgccggaata tcagccatca gctgctggaa ttggttttta gcaaaagaat ctcttaaaca
    35881 aattcttttt ggaccgcttc tggcgaaatc tcgtccccaa tctttttttg aaaagctagt
    35941 ccttttccca tacaaacgac ttcttccccg aatccatttt tcgaaatgac gacattgtta
    36001 ttcaaaattt tatcaatttt cattttgctt cgccctctca agaaaaataa aaaaacctag
    36061 aattcgccta ctgtttctta gctgaattct ggttttgcct gccgtacagt aacaatccat
    36121 ttcttattca aaatcatcat accatcgaat ttttatttat gcaagccttt tcatataaaa
    36181 aataaaaaaa ctggaacaca agtctatcta gaaaacaacc aaatcagaga tgcacctctt
    36241 tgcgtttctc tttttttcag caaattggtt tttccttatc gattcatatc ctttggctgt
    36301 ttttaatctg taacgactta tgtcccagta tcctaaaatg tttttgtctc tagattgaaa
    36361 gcagatctca tatcatctgt cgcatctttt atcgatgtaa cactttccag ttctccttgt
    36421 acaactagtc gcgtgacggc gtccatgtcg ccacaagtaa ttagtgtgac aatttctttt
    36481 ccctcgactt cattcagtaa ttgggtatct gtaggagcaa ctttttcttt aaaaaatgtt
    36541 ttgtatgtat acacattttc taaatctgtc aaatagatct tggcacccat ctctaaattt
    36601 tctagcggtg taaacagcaa ttcagggttc gttgcacgat ggcttgctaa ggcataattt
    36661 ccttttccca tttcttgatc tggagataat gttcctgctc cgtaaagtag tgacgtatta
    36721 tccaagcctt tgaaaaccgg taaattgatt gaaactgaag ggactgcgat actagcaatc
    36781 actggtagat ctgttcctct catttgcgac ctcatgaccc cttctgtcgt cattggttct
    36841 accgcatcaa agtcaaaact gacatcttta tcgttattct ttttcagatc ttcttttgtt
    36901 acattcgcta tagcatattt atctcctgtc tctctgacaa aataatcctt gatctgttca
    36961 ttgaaaatca aagctagtcc tactaataac aagaggaaca agaggatatt gactagccat
    37021 ctttttgctt ttcctttttt tttcattttc tctccctcca gactcatttt actgtttttc
    37081 ttttgctctt tccaacagcc gaatgacaca tttctacaac tctgtgtatt tttttcgtac
    37141 acctgcgctt ttttctacag gatatttcaa attatttttg atcaattttt cttcaatgat
    37201 ctcatctaga tccaggtgaa ggttgtctgc catcatgtat gaatagatca atacatctgc
    37261 cagttcttct ttgattcgtt ctaattgtgt ttcttttact tcttccggcg ttttccattg
    37321 aaaaagttct aacaattcag atgcttccaa agaaatagag atagccaaat ctttttcgtt
    37381 atgaaattgt cgccaattac gttcatctct aaactggttg actttttcca tactattcat
    37441 ttgcttgcct tcttccaatc acttcttact tgattaatca taccattttt ttacataaaa
    37501 aatagatgat gcttttaaaa aaactcctgt tttcttttat ttaaaataaa tgtcatagaa
    37561 aatcaataat ttaggtagcc accatgtgta tgcagtagcg tgtcttcttc ccttgaggct
    37621 ttttgttttt cgatacgttc ttcttcccaa acagacaacg ctactagaat ctctcttaat
    37681 gattttcctt cttcggtgag cgagtactct actttaggcg ggacttggtt ataggctttt
    37741 cgatggatac tttcatcttg ttctaactct cttaactgct gggtcagcat tttttttgtg
    37801 atcgtaggga tttgcttgct tctccacgcc taaaataagc gatattgctc ttccctttat
    37861 ctggtgtaag caaagcagtt agcattttga cgatcgtggg ttccgctttt cacttcttat
    37921 ctacttagta tcatgaagta tactttcgta ctaatactaa gtagcggaag tgtcaactac
    37981 tttgtttaac tacatagcaa aagaaacaga agggtgacaa cggtcatcac gcttctgttt
    38041 cttttgaata tgctgtgttg ttgtctctat ctccgtgtag actttcctca aacttatcct
    38101 ttgacagtaa gtcagtaggc tgatttgctc tttctagatg ccagatacat ttctacaaaa
    38161 aaatagtgat tctgttttga aaattcgatt tttagttctg cattttcgta aacatccaac
    38221 atttttttcg cactgtatag acctaacccg ccattttttt tagaggtttt tcctcttttg
    38281 accatctccg atagcgtcaa attcgttgga tttgccttgt tcgctatact aaaacgtgat
    38341 ccctcttttt cttccagcag attgatcgta atcggactct gatcggctac acgatgatcg
    38401 aatgcgttag aaataaaaat agataaacag cgaataaaaa caaccagatc aatcgggatt
    38461 gttttcactt cgacaggaat aactacttgg atcgggattt tctccgattc tgctttttct
    38521 agaaatttta aaaagacacc cctgacagct ggggatacaa tatttgttag ctgttgtatt
    38581 tgcggttttt caaaaatagc tgcagaatga tcttctaata tttctaaaaa ttccaacgtt
    38641 tcttttttat tttcctgaag agtaattttt aatgaaaaca gtagactttt ataatcatgt
    38701 ttgaattcct ctagatgatg gtaatattct tcatttattt gatacagttg aagcgattcc
    38761 tgatgttgtc tttcttttac ttggaaactg atcaacataa tggaaaaaaa caaagaaaaa
    38821 aaactgatga ctatcgacaa aagagataga agcagataat tagatgagcg gaagtgaaca
    38881 cttcccaata ccaagtaaat cacgccccag cttaataaaa tacaaatacc tccaaaatag
    38941 tgttcagttg gataagtaaa aagcaagttg agaaatctcg tctgtttgtc tatacttttt
    39001 actaatagat agaaaataaa tgcgataagt agtgcaaaaa aacttgctgt tccaagatgt
    39061 ctctttccta aataatccat cagatcaaaa accagaaaaa agctgagtcc agtgatagca
    39121 aaaaaaagat tactgatcaa acagctgata aacaggtttt tcgtagtaaa ataaaaaaga
    39181 aacatcaata ataagccaac taggtaagga aagctcactt tagggatagg aagtaaaagt
    39241 gtcaccacca tcaaagctat acaaccaaag ctgtttttta ctagcagttg tttcgaaaag
    39301 aataaataga tacctaaatc taaaatcaaa gtaaaggtca aataaagtgt cactgttgcg
    39361 accatcttat tcctccattg ttttgtttca aaatgtacac atattttttt aataggagcc
    39421 tgcatatgaa aaagtatttt atcatagaag ataaccagtt tcacagcgaa tggatcatca
    39481 gagaattggt caatcatcat atcgagaata tcgtgcagct cacttctatc tctcaaatca
    39541 atcagttgat ccagttgggc actattgata atcacgatgt tttctttatc gatatcgact
    39601 tgaagcaatc actcacaggt attgatgtcg ccgaacaaat acgtaaaaaa aatgcacata
    39661 gtaatattgt ttttttcacc gcttatacga attttgcagt agagatcatc aatcggaaaa
    39721 tccttccgat tggatatatc agaaaagaca ggaatttatc tgaacaaatt cgtcaagtgc
    39781 tagaacagat caattggaaa gaacagcaga tcgtaaatgt cgataaaatc tttattgatc
    39841 aaaaaggtgg aagtctcgtc ctgtatcctc gggaaatttt atatattgaa agtatcaaag
    39901 gtttgaaaaa ccaagttatg gtcaaaacaa ctacagaaga taaattagtg aatacttctt
    39961 taaaacaatt gaagacagag ttagaaaagt atgcttactt tttactttta aaatcttact
    40021 gtatcaactt atcgcaattg cagaaaattg attctgctca ttatgtgtta gaatttttca
    40081 acggtgactc cttactcgtt ggtcgaaaga tctttgaaaa gacaaaggaa cgtttccatg
    40141 acttccaaaa aactgcttcg agttgatgaa taagagctga gacgtaagca tagcagattt
    40201 cagggagtgg gccttgaatc ggctccactc cctgaaatcg ttgatatgat tttacgcttc
    40261 ctcttctgta ggagcatctg ttaacgacca tgtgaatgtt gaaacgtagt tgtctacagc
    40321 ataattggat acaggaacaa ataattccac tgattgtctc ccttgttcca cattctctcc
    40381 taatgaataa atccatgtgc caatcccttc atttgctcct gcagttacta gatctactgc
    40441 ctgatctgct gggatttcga ttccttcagc gccagttcca gaaacggtag atggcattcc
    40501 actgctatta gttgcgctat ttcgaatcac ggcgtttttg aatttcacct ttgaaccagt
    40561 tagtttattc gtatcagatg ttgtaccaat aaattctgtt cgtttgactg ataatttcca
    40621 accggcataa tttccgcttt tatccgttac ttgtacgaaa ttctgtactt gtgtttgtgc
    40681 tccagtactg tccgttccgc tttgtaatgc agcatatact gtttttgctg tacctaactt
    40741 gattgttcca aaatcaagat ctggagcttg gtcaatcgtc agtaatccgg atgttccctg
    40801 atttggttcg tcaggtgtaa aaggagtatc aggatcattt ggatcaacag gaggaactgg
    40861 aacaacgtct tcaggatttg cagcatctgg tttggtcaca gaaatcgttc cttctgtagt
    40921 ggctgagctc ttattctcag caaaaacact ttgtgtacat aataatagac ttccagctag
    40981 aacggtactt aatgcaattg tttttttcat ttctttttcc ttctttcttt taaaataaat
    41041 aatccagcaa ataagccaat aagaatgact ccaacaagta tcagccatcg ccagtcaaat
    41101 gtttctggct gattttttaa atcaatatcc ttttcattca attggtccgc tgcatcatca
    41161 gttatttcaa aatcggtatg gagattccag cgtttttctt tccaagcacc cttcacaacc
    41221 gcttgataat gtcccggagc taattttttt ccttctaatg ggacaagtaa atcaaaattt
    41281 gtattgggtg ctacatctat tttttctttt tttattgaaa ggtaaggctc ttttgtttct
    41341 tttttgtaga tttcaaccga tgtagtcaag ccgaacatat aagaagcatt tggattttga
    41401 agattcgcct taatcacatt tcttccatcg ctttgatccg cataagcttt ttttaatttc
    41461 aaatctgggg aagactcttt ttcgctattt caaatcaaga ttgctttcac ttgagaaaac
    41521 tgattgacga caccaactgt acttttgtta gaaacgttct cttttttttc ttgaaacgtg
    41581 atccctcctg cgaccacacc agccagctct ttttctggcg ctttcagtga aagtgtctca
    41641 gtgaccgcac tttgaggagg aacagttatt tctcccactg tttttgctaa gtttttcata
    41701 tcataactta atgtttcatc aaatttactt tttgattctc catattcgat cacaccgttt
    41761 gaattcgtcg tagcggagtg gatcaatgtc tcaactgtta ctgatttatc tgttcggtta
    41821 aagaccgtgt aggggatttc ctgtgttttc ccaggctcca tccttacatt tatatatgtt
    41881 ttgttcttat caatctgatt agccggtaat tcaaaatcta ccgcaaaatt gaattcggag
    41941 gcatggacag gtgtctgcca aaggatgaat ccaaggagaa ggatcataag taaagagcag
    42001 ttgtttcgat gacttttatt ccatattcga ttcatcaatc atcctccttg atctttattc
    42061 tgtcggcgca tctgctaatg tccatgttat ctctgctgag taattaccag ctatctgatt
    42121 ttctttggtt aagacaagtt taaacgcccg accgttattt aaatattctg aataatctgc
    42181 ttgcacattc gttgaatttt gtcctctgac aatttccagt tcttcttcat tcactaattg
    42241 atactcctct cctttcttga aatataggac atcctgcata gaaaatcctg catcatttgt
    42301 gaaaggctga gataatttta tcgttaagat ccacggattt ttcgtttctc gctgatcata
    42361 aatccataat ttttcctgta tatccatttg gccaaatgct tcttgtgtca aaccagttgc
    42421 tttgatctga ccaaaatcga gtttggaagg ggcaagaaat gagagaccac cttctactac
    42481 gatcttcgta gcaggtgcaa aaggcggggc atcatgataa acaactggcg tatcacttgg
    42541 attgtgattc ccaatttcat cgttggtact tggcttgttc tgcacaccct tagcagtagc
    42601 ttctcctgct tggtcgttta atacaatttc taatacatct cctgcaaaaa gttgattttc
    42661 tgggatagaa agaacaaatt cccctgtatc atcaatagtt gtttctgaca aaacagcttg
    42721 attttttttc aatgaaacca aggctcctgc ttcacctgta cctgtgatcg tgcgagaagc
    42781 tgcattgatc aatgtggtac ttaaattagc aggttcagga ggaatcacat cgactgtcgt
    42841 tttggcagag tccagttctg tcacttcttg gccacctctt acttttttag cagcaacaac
    42901 ttgcacttga tcctccgttt ttaatctctc atcattgggt acttgaactt cgaacaagcc
    42961 gccttgttcg ccttctcccc atgggttata tcctgcccct tcattttcat ctgtttgtcc
    43021 ttgcgtgaga gcagtaattt ctacagatga tttcgtcgta tctgcgtaag taatacgtaa
    43081 agtcacttct acttcgcctg tccaagcatc tcttggcttt gcctcgacac cttcgggaac
    43141 aacgacatgc ccatagattt ttttatctgc attcgttggc actcttaagg attcaatcat
    43201 cgggagctga ttattggcac tgattcgatt ataatttttc attccggcac ctgtctgagc
    43261 aaattcattt gttaaacctg cactagggct tgatacgaaa ttgcctaagt cggtcccatt
    43321 gatcgataag tctgtcatat cagaaaaata atccgggtct ttttcaaagt tatcgaatgt
    43381 tgcgcctgtt tttcgccata aagcgatttc tgaatgggcg atctttaaag aagaatctcc
    43441 atttccttgg aatacccagc cgcctatgcc attggtctcg cttccaatcc gtttgttttg
    43501 gaaatcaaaa taggtaggag attctaagtc cacattcaac acatccccac tgatgatgcc
    43561 ggtcttatct tcccctgttt tcccgaccat gactaaataa gttttttcac ctgccgtaaa
    43621 atttaaatca atatctccag ttgtatctac agttgctccg gaatctgcgc gtatatcgat
    43681 atttgatttt tctccagtga ctgtaaaatc agctgaacct ggactttcta gaaaaccaga
    43741 agcgtcataa aattggatcg cttgatttcc ttgattgagt ctgttcacgt aagatttgcc
    43801 atcacctgta tttcttaccc tcaatgagcc tccattggtt actgtcagct tttgcgtacc
    43861 attctcaaaa cgaactgcgc tggaacgccc actctgctta tcgatgtcga taactgatcc
    43921 ggctccttct acgatcaaag aaaggccttt tcctacaaaa cgcatcccat tgttcccggg
    43981 ttgatcgtca tctccagcca ttttgaccga gatttgtcca ccacttgata catggatttg
    44041 actagaatct ccttgcaagc gaaatccgcc cgtagttgct gtattgatgg tgattttccc
    44101 accattcgat acagtgactt tcgatcctga acctctcatt tggatgattc cgcgattgtt
    44161 gtcattggta atattgatct ttgtatttac cgaaaattcg cttcccgaac cactgacttc
    44221 aaacatgaca ttaccaggta gcgtatcgac tgcctctcct tcatcgctaa cgccatctgc
    44281 ccgatcataa tcaagagaga cctttgaacc gcttaagata tggattcctg gcgctgtttt
    44341 tccactacca gtctcatctc ctgaatctag aaaaccgtag aatttcccta gatacggtcc
    44401 ataaagttga ctaccattga tcacattcaa ttgatagctt ctacaaaaat aggctttacc
    44461 cggagcggca ttcagtcctt ccttcactgg ttggacatcc aacacaccgg aaacaccatc
    44521 taatgtgaca gttgcacgag gagcgtaaat cagtcctaat ttggacccat catacaaatc
    44581 aagtgtcccg gtaaatttta tttctcccgt aaaatgatat ctgcttcccc aaaaatcggt
    44641 catataagca atgataatgg catttccatc gatatttggt gtcaatccac ctggatgtgc
    44701 agctgttcct ttaaaattct ggaactcgat cgactgaggt atctcattga tataaatatg
    44761 gtgtgtgccg aaattgtgag cgaatccacc aaaatcaata acgatatctc gtttgatctc
    44821 taattccctg tctaaagtca aaggctgaat cagtcggatc ttagttactg attcgtctgc
    44881 gagaagctgc gccagttctt ctgttgtggc agcttcgctg tacccatccg ctgaaacagc
    44941 tcttgccagt gccgcttcac tgctatcaga cgtttgttca gctacttctg aggtctcact
    45001 tgtcttattt tcttcttgcc ttttattagt agaagcctca ctttgagatt ctgtagtttc
    45061 agaagtaatc gattcttctg aaaaagtagt cttgtcaaaa ctttgattct ctgattgcgt
    45121 tgattcgttt gttttctcta catcggcaaa aacggatgtc accatcaaga gagggaagac
    45181 aaacaaaatc gacgcaatca gcttttttac acattgattc attctccacc atcctctctg
    45241 acgttttgac aattttattt tatcgtttta atctatttcc aattgtactt tcatatttaa
    45301 aatatgtatt tttatggaaa aatttttcta ttaggtgtaa atttcttttt tgtacaaaaa
    45361 aaaggctgtg acaaaagtct tgtcacagtc ccttatcccg aacaagcggt gttctcccgc
    45421 caactcctcg gtcacaacct ccattttaac aataaacctg taaatgtgag ggcaatactt
    45481 tgattgtcac aggtaattta tccccttcat ctccatcaac attcgtcgtc aattcttcct
    45541 catcagctaa tgacacaact aattgctcac aagtaaggta ggctagattt ttcgtagatt
    45601 catctactcc ttttaatagg tctggcaatg ttttaagcgt atcgatcaaa gaagaatctt
    45661 ttaaataaac caaatggagc ttaccatcat ccacttttgc atttggaagt aatgtttcaa
    45721 aaccgccgac tgagttagtt agtcctatca gcaaggtact gctctcaatt tcttccttct
    45781 ttccatcaac ttctacgtga aacgagtagg attgtgtgga agctaactgt ttgattcctg
    45841 acatgaaata agctagtttt ccaaatttcg tcttcttctc cgcatctaca tcattgattg
    45901 attcagtgat agagccaatt gcgacgacat tcataaaata gtcgtcattt atttttccaa
    45961 tatccaatgg cttgactgat tcaatcgaaa aatgattgat cgcttcctca ggttctagcg
    46021 gtatccctaa tgctcgagcc aagtcattca ccgtacccaa tgggaaaaat ccgaaatttg
    46081 gccgatgttc ttgttcagcg attccactga ttccttcgtt gactgttcca tctccgccca
    46141 tgacaaacac actatgatac ccttccgtag ccgcttcgcg agtaaagttt tttgcatccc
    46201 ctgctttttt tgtatgcaag acgaccactt catcaaacac cgattccaat tttgctatcg
    46261 ctaattgctc aaattccttt gcttgttcac cgcctgaact ggggtttacc actaataaaa
    46321 cttttttcat cactttcttt cccttctgat tgatttcttt aatataacga tagcataaag
    46381 aaaaaaatta ggctgtgatg aagtactgtt catgaaacat tcatttattt tttgacaaga
    46441 taaaacatcc atactatgaa actgaggaaa attaaaacaa tgatatagga aataaaagaa
    46501 aatgataggc attgtatacc taataaagta aaaatcttag gtggcagata aaaacacaaa
    46561 aatagcaaga gaatgtaata gcgtttcttc cactttttgt tatcagacct tatagcgatc
    46621 agaggaatac caaatgcata actgaaaaac tcttgatgta ctgttaatat gtcacggcct
    46681 tgaataataa atgcgacaat ccccgctata attagtgtgc ctaacaccaa aacggaaaat
    46741 tgatgaatat attttatata caaatgatcc attgtacttc ctgccttttt atttgtgaga
    46801 aaaacaaatc cgtatacgtg aataacttct agaaataaa
//
LOCUS       DAYYGS010000011        46517 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000011 DAYYGS010000000
VERSION     DAYYGS010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.011"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            61..453
                     /locus_tag="ACHA44_000582"
     CDS             61..453
                     /locus_tag="ACHA44_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8903899.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            481..1821
                     /gene="glnA"
                     /locus_tag="ACHA44_000583"
     CDS             481..1821
                     /gene="glnA"
                     /locus_tag="ACHA44_000583"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFM8903900.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            2547..2792
                     /locus_tag="ACHA44_000584"
     CDS             2547..2792
                     /locus_tag="ACHA44_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903901.1"
                     /translation="MKEKDCFYDKLYKLLLQVGKSSSLLGGILSIIMFVLFFYLLFHL
                     KFFSNDFLICLGLTLAMFISSLLCGLNFFCTHKGLKK"
     gene            3014..4624
                     /locus_tag="ACHA44_000585"
     CDS             3014..4624
                     /locus_tag="ACHA44_000585"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFM8903902.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4601..4813)
                     /locus_tag="ACHA44_000586"
     CDS             complement(4601..4813)
                     /locus_tag="ACHA44_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903903.1"
                     /translation="MKMKIFKKFFAKNKISNSALIEKSLLLSIFFPTNNNKSEKEKSD
                     KKIAYPLLLLLFSFYQVQKLFCFVIC"
     gene            4916..5785
                     /locus_tag="ACHA44_000587"
     CDS             4916..5785
                     /locus_tag="ACHA44_000587"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFM8903904.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            5950..7221
                     /locus_tag="ACHA44_000588"
     CDS             5950..7221
                     /locus_tag="ACHA44_000588"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8903905.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7218..8513
                     /gene="rho"
                     /locus_tag="ACHA44_000589"
     CDS             7218..8513
                     /gene="rho"
                     /locus_tag="ACHA44_000589"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFM8903906.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8612..8875
                     /locus_tag="ACHA44_000590"
     CDS             8612..8875
                     /locus_tag="ACHA44_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFM8903907.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9320..9769
                     /locus_tag="ACHA44_000591"
     CDS             9320..9769
                     /locus_tag="ACHA44_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8903908.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9826..11244
                     /locus_tag="ACHA44_000592"
     CDS             9826..11244
                     /locus_tag="ACHA44_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8903909.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11244..12104
                     /locus_tag="ACHA44_000593"
     CDS             11244..12104
                     /locus_tag="ACHA44_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8903910.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12278..13051
                     /locus_tag="ACHA44_000594"
     CDS             12278..13051
                     /locus_tag="ACHA44_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8903911.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13198..13563
                     /locus_tag="ACHA44_000595"
     CDS             13198..13563
                     /locus_tag="ACHA44_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HFM8903912.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13711..14919)
                     /locus_tag="ACHA44_000596"
     CDS             complement(13711..14919)
                     /locus_tag="ACHA44_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8903913.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(14938..15216)
                     /locus_tag="ACHA44_000597"
     CDS             complement(14938..15216)
                     /locus_tag="ACHA44_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903914.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15409..16065
                     /locus_tag="ACHA44_000598"
     CDS             15409..16065
                     /locus_tag="ACHA44_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8903915.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(16507..17304)
                     /locus_tag="ACHA44_000599"
     CDS             complement(16507..17304)
                     /locus_tag="ACHA44_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFM8903916.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17301..18485)
                     /locus_tag="ACHA44_000600"
     CDS             complement(17301..18485)
                     /locus_tag="ACHA44_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HFM8903917.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18510..19757)
                     /locus_tag="ACHA44_000601"
     CDS             complement(18510..19757)
                     /locus_tag="ACHA44_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8903918.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            19972..21717
                     /gene="dnaX"
                     /locus_tag="ACHA44_000602"
     CDS             19972..21717
                     /gene="dnaX"
                     /locus_tag="ACHA44_000602"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFM8903919.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21737..22051
                     /locus_tag="ACHA44_000603"
     CDS             21737..22051
                     /locus_tag="ACHA44_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFM8903920.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22076..22672
                     /gene="recR"
                     /locus_tag="ACHA44_000604"
     CDS             22076..22672
                     /gene="recR"
                     /locus_tag="ACHA44_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFM8903921.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22736..23380
                     /gene="tmk"
                     /locus_tag="ACHA44_000605"
     CDS             22736..23380
                     /gene="tmk"
                     /locus_tag="ACHA44_000605"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFM8903922.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23395..23724
                     /locus_tag="ACHA44_000606"
     CDS             23395..23724
                     /locus_tag="ACHA44_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HFM8903923.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23738..24676
                     /gene="holB"
                     /locus_tag="ACHA44_000607"
     CDS             23738..24676
                     /gene="holB"
                     /locus_tag="ACHA44_000607"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFM8903924.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24712..25536
                     /locus_tag="ACHA44_000608"
     CDS             24712..25536
                     /locus_tag="ACHA44_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HFM8903925.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25529..25876
                     /locus_tag="ACHA44_000609"
     CDS             25529..25876
                     /locus_tag="ACHA44_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HFM8903926.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            25945..26817
                     /gene="rsmI"
                     /locus_tag="ACHA44_000610"
     CDS             25945..26817
                     /gene="rsmI"
                     /locus_tag="ACHA44_000610"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFM8903927.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            26926..28047
                     /gene="dinB"
                     /locus_tag="ACHA44_000611"
     CDS             26926..28047
                     /gene="dinB"
                     /locus_tag="ACHA44_000611"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFM8903928.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28101..28703)
                     /gene="nrdG"
                     /locus_tag="ACHA44_000612"
     CDS             complement(28101..28703)
                     /gene="nrdG"
                     /locus_tag="ACHA44_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HFM8903929.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29018..31207)
                     /gene="nrdD"
                     /locus_tag="ACHA44_000613"
     CDS             complement(29018..31207)
                     /gene="nrdD"
                     /locus_tag="ACHA44_000613"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFM8903930.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31533..32300
                     /locus_tag="ACHA44_000614"
     CDS             31533..32300
                     /locus_tag="ACHA44_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903931.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32290..34332
                     /locus_tag="ACHA44_000615"
     CDS             32290..34332
                     /locus_tag="ACHA44_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8903932.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34613..34762
                     /locus_tag="ACHA44_000616"
     CDS             34613..34762
                     /locus_tag="ACHA44_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HFM8903933.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34773..36287
                     /gene="dltA"
                     /locus_tag="ACHA44_000617"
     CDS             34773..36287
                     /gene="dltA"
                     /locus_tag="ACHA44_000617"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HFM8903934.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36284..37489
                     /gene="dltB"
                     /locus_tag="ACHA44_000618"
     CDS             36284..37489
                     /gene="dltB"
                     /locus_tag="ACHA44_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HFM8903935.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37534..37767
                     /gene="dltC"
                     /locus_tag="ACHA44_000619"
     CDS             37534..37767
                     /gene="dltC"
                     /locus_tag="ACHA44_000619"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HFM8903936.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37770..39038
                     /gene="dltD"
                     /locus_tag="ACHA44_000620"
     CDS             37770..39038
                     /gene="dltD"
                     /locus_tag="ACHA44_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HFM8903937.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39179..40261
                     /locus_tag="ACHA44_000621"
     CDS             39179..40261
                     /locus_tag="ACHA44_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HFM8903938.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40261..41064
                     /locus_tag="ACHA44_000622"
     CDS             40261..41064
                     /locus_tag="ACHA44_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8903939.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41134..41874
                     /locus_tag="ACHA44_000623"
     CDS             41134..41874
                     /locus_tag="ACHA44_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8903940.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            41885..42889
                     /locus_tag="ACHA44_000624"
     CDS             41885..42889
                     /locus_tag="ACHA44_000624"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HFM8903941.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(42934..44778)
                     /locus_tag="ACHA44_000625"
     CDS             complement(42934..44778)
                     /locus_tag="ACHA44_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903942.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            44980..45507
                     /locus_tag="ACHA44_000626"
                     /pseudo
     CDS             44980..45507
                     /locus_tag="ACHA44_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            45635..46318
                     /locus_tag="ACHA44_000627"
     CDS             45635..46318
                     /locus_tag="ACHA44_000627"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HFM8903943.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
BASE COUNT        14993 a         8078 c         9678 g        13768 t
ORIGIN      
        1 gacaatattt tttttaattg gtacacttta gccaacttat aaaacgaaag gggacgtctg
       61 atgggagaaa aggaattgcg ccgatctatg tccgtatttc caattggtac agttatgaag
      121 ctgactgatt tgtctgctcg gcaaattcgc tattatgaag aacaagattt agttcatccg
      181 gaacgaagcg aaggaaaccg acgtatgtat tcgctgaatg atattgatat gttgcttgag
      241 attaaagact atctttcaga gggattgaat atggctggga tcaaacgtgt atacgaaatg
      301 gcacaggcga aaaaacaaga agcaaaaaac aagaagccgt tgacagatca tgacgttcga
      361 caaatttttc gtgatgagat tttggcgcaa ggtgggttaa gcaaaacagg tcattatcag
      421 tcgcaaggat tgcaaagaca attctttgac tgaataaatt aaaaaaggaa gagggatacc
      481 atggtaaaga aacagataac aggtgaagaa atcaaacgaa tcattgagga agaaaacgta
      541 cgatttttac gattgatgtt cacagacatc ttaggtacga tcaaaaatgt agaagttcca
      601 gtgagccaga tcgacaaagt attagaaaat aaaatgatgt ttgacggttc ttcgattgaa
      661 ggatttgttc gaattgaaga aagcgatatg tatttgtatc cagatctctc cacttggatg
      721 atcttcccgt gggaaagtgc tcatggaaaa gttgctcgtt taatttgtga tatctataat
      781 ccggatggga caccatttgc cggcgatcct cgcgggaatt tgaaacgagc attgaaagat
      841 atgcgcgacc ttggttttac ctctttcaat ttaggacctg aaccagaatt cttcttattt
      901 aaattagacg aagacggtgg gatcacaacg accttgaatg ataaaggtgg ttattttgat
      961 tttgcaccaa cagatttagg tgaaaattgc cgccgtgaca tcgtgttgga attagaaagt
     1021 ctaggctttg aagtggaagc ttcacatcat gaagtagcgc caggacagca tgaaattgac
     1081 tttaaatatg cagatgtggt agatgcttgt gataatatcc aaaccttcaa gctcgttgtc
     1141 aaaacgattg cacgcaagca tggacttcac gcgactttca tgccgaaacc gttatttggg
     1201 atcaatggtt ctggtatgca ttgcaatatg tctttgttca atgatgaagg aaatgttttc
     1261 tatgataaag acggtgaatt ggaactaagt gaaacagctt atcatttctt aggtggtttg
     1321 ctgaaacatg ctcgtgccta cacagccgtt tgcaatccaa cagtcaattc ttataaacgt
     1381 ctcgttccag gttatgaagc gcctgtttat gtcgcatgga gtggccgtaa tcgttcgcca
     1441 ttagtacgtg taccagaatc tcgcggttta tccacacgtt tggaattgcg gtcagttgat
     1501 ccatctgcaa acccatactt ggcaatggca gtattgttgc aagcaggact agacggtatc
     1561 cgtaatcaga tcacaccgcc agcagctgtt gaccgtaaca tttatgtaat ggatgaggaa
     1621 gaaagagaag ctgcacatat ccaggactta ccttcaacta tccataatgc tatcaaagaa
     1681 ttgcgtaagg ataatgtaat gattgacgca ttaggacaac atatcttctc aaattttgtt
     1741 gaagcaaaac gtctcgaatg ggcggcattc agacaaactg tttccgagtg ggaaagagaa
     1801 caatatctag aactttatta aaacaggaat accgtgaatt agaggctgtg aaatcagcgt
     1861 tttgacctga gtattagaga aactaacgga aaaatagttc ctcatatttt gccgtttttt
     1921 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaggttg tgaaaaagct
     1981 gccctgcaac aagtaataag aatagccaag caaaaacagc ttttcttgtt tttgaattgg
     2041 ctgaacttat taccgcagat gcaagctttt gaacaccgtt taccaagagg ttttgaaaaa
     2101 gacgtttagt ttcgagtatt aagaaaaatt ttgtgaaaat ggctttctac atttttgcat
     2161 aaatttggct taataccgag aaactggctt ttgaataccg tttatccaga ggttgtgagc
     2221 ctaccattta actccgaatc acaagaaata atttcaaaaa cagcttctca tgtttgatgg
     2281 atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttatttag aaataaggag
     2341 gcacgacttt tgtcgcatct ccttattttt tacttatgtg attcgttacc ttcaatgaat
     2401 ccctacaagg cacttttgtt gaaaaaaagg tcatctttga aaaaattagt ggaattgttt
     2461 tgtttttgat attatttaat agaaaagata tgccaaaaat ccgagaactg tggactacgt
     2521 tttttacgta aaggaggtaa ataagggtga aagaaaaaga ttgtttctac gataagctat
     2581 ataaactgtt gctgcaagta ggaaaaagta gttctttgtt aggtgggata ttatctatca
     2641 taatgtttgt tttatttttt tatctattat ttcacttaaa atttttcagt aacgattttc
     2701 tgatttgctt aggtttaacg ttagctatgt ttatatcaag cttattatgt gggttaaatt
     2761 ttttttgcac tcataaagga cttaagaaat aatttctcaa aggatatcga aaaagaaaaa
     2821 attgtaagcg gatagtattc atttgagtac ttgactttcc cttagtcagc ttgtattata
     2881 caccttgggc acccttttag agggtcagaa gtttacagaa atttcggctc cctattctta
     2941 ttgaagaata gggagctttg tttattgtct gagacttttc ctgcacataa attaaaaagg
     3001 agtttttgaa cttatgacaa aatatatttt tgtgaccggc ggtgtcgttt catcaatcgg
     3061 aaaagggatt gttgcagctt ctttaggacg cttattgaaa aaccgcggct taaaagtaac
     3121 tatccaaaaa tttgatccat acatcaacgt ggatccgggg acgatgagcc catatcagca
     3181 cggagaagta ttcgtaacag atgatggtgc tgaaacagat ttggatctgg gccactacga
     3241 acgttttatt gacatcaatt tgaacaaata ctccaacgtg acgacaggaa aaatctattc
     3301 tgaagttttg cgtaaagaac gtaagggcga atacttggga gcaactgttc aagttattcc
     3361 tcatattaca aatgagatca aagaaaaaat catgcgagca gctaaaatga ctgattcaga
     3421 tgtgatcatt acagaagtag ggggaacagt aggggatata gaatctttgc cattccttga
     3481 agcacttcgt caaatgaaag cagatgtcgg tacagataat gttatgtata tccatacaac
     3541 attgattcct tacttgaaag ctgctggaga aatgaaaact aaaccaacgc agcacagtgt
     3601 aaaagagcta cgtggtttag gtatccaacc gaatattttg gtggtacgta cagagcaacc
     3661 tgtttctcaa agcgtgaaaa acaaattggc acaattttgt gatgtcgaac cagaagcagt
     3721 tatcgagtcg ccagatgtag atacacttta ctccatccca ttagttttac aagcacaagg
     3781 aatggatcaa attgtttgcg accatttaaa actagataca ccagaagcag acatgacaga
     3841 atggaaagaa ttagaagaac gtgtcttgaa cttgaagaaa aaagtacgga ttgcgcttgt
     3901 cggtaaatat gtcgagttac cagatgcata tatctcagta gttgaagcat tgaaacattc
     3961 tggtttcgcc tataattcag acatcgagat cgattggatc aaagcacaag aattaacgag
     4021 agaaaatgta gaagaacgtt tgaaagacgc agatggaatc ttggttcctg gtggatttgg
     4081 tgatcgcgga gtagaaggga aaatcgaagc gattcgttat gctcgcgaaa atgacgttcc
     4141 gtttttaggt atttgtcttg gtatgcagat ggcttgtgtc gaatttgcac gtaacgtcgt
     4201 tggattagaa gatgcgtcat cagctgaaac gaatccagat acagcaaaca atattattga
     4261 tttaatggct gatcaggaaa acatcgagaa tttgggtgga actttacgcc ttggattata
     4321 cccatgtaaa atcaaaaaag gaacgaagac ggctgaagca tatgatggag ccgatgttgt
     4381 gcaagaacgt caccgtcacc gctatgagtt caataacaaa tatcgtcaat tgtttgaaga
     4441 acaagggcta gtcttttcag gtgtctcacc agataaccgt ttagtagaaa tcgtagagct
     4501 gccagagaaa aaattctttg tgggatgcca attccaccca gaattgattt ctcgtccaaa
     4561 tcgcccgcaa aaattaatca aagcatttgt cggagcatca ttagcagatc acgaaacaaa
     4621 ataatttttg aacttgatag aaagagaaaa gtagcaagag aaggggataa gcaattttct
     4681 tgtcactttt ctctttttct gatttgttat tatttgtagg aaaaaagata gaaagcaaaa
     4741 gacttttttc gattaaagcg ctattggata ttttattttt tgcaaaaaac ttcttgaaaa
     4801 ttttcatttt cattgatttt agtagaaaac taccgaaatg tttggtaaaa tagggtcgtt
     4861 ggttaagaat tgtcttaaca aatttagttt tacaaaactt aggaggaatt ttattatgcc
     4921 agtagtatca ggagctgaat ttttaaaagc tgctcgtaaa ggcggttacg ctgtcggcgg
     4981 attcaacaca aacaacttgg aatggactca agcaatcctt gaagcggctg aagcaaaaca
     5041 agcaccagta cttatccaaa catcaatggg tgcagctaaa tacatgggtg gttacaaaat
     5101 ctgtaaagat ttaatcactg atttagttga agcaatgaac atcactgttc cagttgctat
     5161 ccacttagac catggtgatt acgaagctgc tttggaatgt atcgaattag gctatacttc
     5221 agtaatgttc gatggttcac atctaccatt tgaagaaaac ttgaaattag ctaaagacgt
     5281 tgttgaaaaa gcacatgcaa aaggtatctc agttgaatgt gaagttggtt caatcggcgg
     5341 agaagaagac ggaatcatcg gtaacggcga attagctgat attgaagaat gcaaacaaat
     5401 ggttgctaca ggtatcgact acttagcttg cggtatcggt aacatccatg gtcaatatcc
     5461 agaaaactgg aaaggattag cttttgacca cttacaagca attgctgatg ctgtaggtcc
     5521 agacatgcct ttagtattac atggcggatc aggtattcct caagatcaaa tcgaaaaagc
     5581 tatctcaatg ggtatctcaa aagtaaatgt taacactgaa ttccaattat catttgctgc
     5641 agctactcgt aaatatatcg aagctggtaa agataaagaa ggtaaaggat tcgaccctcg
     5701 taaattatta gctccaggta aagcagcaat cgttgaagat gctaaatctc atatcgactg
     5761 gttcggttca gctaacaaag cgtaagcatc cacataacag atacgatata taggaatgga
     5821 gaacgacttt tgaagtcgct ctccattctt ttttacttaa aacggcagac catgctcgtt
     5881 tctttttttt atctatgata aaatacaagc atatccatga aaaaagatta ataaacaagg
     5941 tggaaacaga tgaaaaaaat cgtaatcaaa ggaaaccgac ctttagctgg tgaagtaacg
     6001 atcagcggtg caaaaaatag tgcagtcgca ttgattccag ctgcaatctt agctgattcc
     6061 ccagtgatct tggaaggtgt gccggacata caagatgttc attcattaat tgaaatttta
     6121 gagatcatgg gcgcaactgt ccatttttcc aacaacatac tagaaatcga tcctcgagaa
     6181 gtcgtatcga ttccgatgcc gcacggaaaa atcaatagtt tgcgtgcttc ttattatttt
     6241 atgggaacac tgcttggtaa atttggcgaa gcggtagtag gcctgccagg tggctgttat
     6301 ttaggcccta gaccgattga cctacacgtc aaaggatttg aagctctagg tgctgaagtg
     6361 acaaatgaac atggtgccat gtatttacgg acagaaaata aaggattgcg tggcaatcgg
     6421 attttcatgg acgtggtttc agtcggagca acgatcaatg tgatgttagc tgctgtaaaa
     6481 gcaaaaggac agacagtaat tgaaaacgct gcacgagaac cggaaatcat agatgtggca
     6541 actttactga ataatatggg ggcaaaagtt cgaggagcgg gtacagatgt gatccgtatc
     6601 gaaggagtcg aaacattaca tggctgtcgg catttcatga ttcctgaccg catcgaagca
     6661 ggaacctatc tagcattagc agctgcagta ggaaatggta tcaaagtgaa aaatgtgatc
     6721 tttgaacatt tggaaagctt tatagcaaaa ttgcaggaaa ttggtgtcca tatgaaaatc
     6781 agcgaagatg aaatcgaagt ttacccctcg aaagaattaa aacccgcaaa catcatgacc
     6841 tatccttatc caggatttgc cacagatctg cagcagccac ttactgcgtt actattgatg
     6901 actacaggca cttcagaaat catcgatacg atctatgcga aacgtgtgaa ccatgtgccg
     6961 gaactagcac gaatgggcgc tgatattacc gtggaaggaa atatgatcat cgtgaatggt
     7021 ccaaataaac ttcacggcac agaagtcgtg gcttctgatc ttcgggcagg tgcttgttta
     7081 gtgattgctg gactattggc agaagggaca acgacgattt acaatgtaga ttatatctta
     7141 cgcggttatg atcatattat cgagaaacta acagccttag gggcatcaat tgaaatgata
     7201 gaggaagtgg gagctgaatg agcgattatt tgacaatggc agaactagag aacaaaacgt
     7261 taaaagaagt ttatagttat gccaaagaat ttaaaattcc ttattacagt aagatgaata
     7321 aaaaggaatt atctctagct gtgattcgtg cacaagcaga gaaacaagga ttttattata
     7381 tggaaggaat attggatatc gttggtcaag atggttacgg ttttttacgg ccaatcaatt
     7441 atggaccaag tgcagaagat atctatattt ctgcctcaca aatcagacga ttcagtttga
     7501 gaaatgggga caaagtggca ggaaaagccc gtccacctaa agaatcggaa cgttattatg
     7561 gtttgatgca cgtggaaagc gttaatggaa aagatccgga agaagcaaaa gaacgtccgc
     7621 attttccagc cttaacacct ctttatccgc aaagacagct tcaactggaa acaacaccgc
     7681 aaagactatc tacccgaatg atcgatatct tttctccaat cggttttggt cagcgtggat
     7741 tgatcgtcgc accgccaaaa gcagggaaaa cttctgtatt aaaagaaatc gcaaatggta
     7801 tcaccgaaaa ctatccggaa gtagaattaa tcgttctact gattgatgaa cgaccggaag
     7861 aagtaacgga tctagaacga agcgtgaaag gcgacgtggt atcttctaca tttgatctgc
     7921 agccgcaaaa ccatactcga gtttctgaat tagtattgga acgcgcaatg cgtttagtcg
     7981 aagataaacg agatgtcgtt atcttgatgg atagtattac tagacttgct cgggcctata
     8041 acttagtcgt tccaccaagc ggacgtacat tgagtggcgg gattgaccca gccgcattgt
     8101 ataaacctaa gaaatttttc ggggcagcaa gaaacatcga agaaggtggc agtttgacga
     8161 ttttagcgac cgctttagtc gatacaggaa gccgtatgga tgacgtgatc tatgaagaat
     8221 tcaaaggaac tggaaatcta gaattacaac tttcacgtga attatctgaa cgtcggatct
     8281 tcccagccat cgatatcaaa aaatcaggta cccgaaaaga agaattattg atgccaagtg
     8341 aagaattaga agaaatttgg aaacttcgaa aaaatatgac tggagattca ttagaatata
     8401 cggaacaact gatccgcttt ttgcgtaaaa caaaaaacaa tcagcaattt ttcaaagaat
     8461 ttcaagatgt ctcctttggt aaaaaaaatc cgaccagaaa tctgaaaaga taattgcaac
     8521 cttgactgaa acatgttaag atgttaatgt tgtaaatcga caaatcaact ctggttcagc
     8581 aaatggagca gggcaaagga gcgaaaaaat catgaaagaa aacatccatc cagattacca
     8641 cccagtagta ttcatggatt caacaactgg tttcaaattc ttgtcaggtt caacaaaaac
     8701 ttcacaagaa acagttgaat gggaagacgg taatacatac ccagtcatcc gtgtcgaagt
     8761 aacttctgac tcacatccat tctacactgg acgtcaaaaa ttcacacaag cagacggacg
     8821 tgtggaccgt ttcaacaaaa aatacggtct caaagacgca aacgctgcgg aataataaaa
     8881 acgctgataa ctcaacgttt agcagactat ctgataggga tagtctgttt ttttatgttt
     8941 tttgccaatt taaaacttcg tttgatgctg aaaaagtcgt aggatcataa tgcacaaaac
     9001 aatgaaaaac tagtatatag ttttaattat ccacttgata aaaacacctt atattaacga
     9061 aatgaagagg ggttatttcg aagtaaaaaa ccatttacct aaaattagaa gctaagcttt
     9121 cctttcaagg aaggcttttt gttttgttat agacttgcga ttgtttgtga gctagatcgt
     9181 tcattttaat gacttataca cgcggatgat gggtcttttt cctctgttca ttcataatat
     9241 tgaacggtac agctagcgat gctatactga cctcagttcc aagaaaacgt taacattttt
     9301 tgtgggggag aggtttttta tgaggtttga tcaattgatc gcagaagaac tgattgtttt
     9361 cgatccatcg atagattcaa aaggatcatt atttgagcga ataggggatg tgttgaaaaa
     9421 aacaaataag gtaagaaatg ttcaaaaagt cgttaaggca ctattcaaac gagaggagga
     9481 gatctccaca ggaatagaag atggatttgg gattccccat gcaaaaagca aatatgtgac
     9541 aaaaccagca ctgtgttttt ttcatacagc caaattaacg gactatagcg ggatagatga
     9601 aatgccaatt gaatatattt ttgccatcgt agttcctgaa aaatcagcag atatgcatct
     9661 agagattctc agcacactct ccagacgatt gatgaatgca gattttagaa agggaataaa
     9721 agaagccact acgccagcag atatcatcag acagattagt caagaatgaa aatagtagag
     9781 tctttgtccg agtatcgata gtgaagaaaa aggaggaaaa taatcatgaa aatcgttggt
     9841 gtaactgctt gcccgactgg aattgctcat acttatatgt cggcagaaaa attagaagta
     9901 acagcaagaa atctaaacca tcaagcaaaa ttcgaaacac aaggtgtaaa aacagaaaat
     9961 cagttatctg aacaagaaat caaagaggca gatgcaatca ttcttgcagt agataaagaa
    10021 atcgaattgg accgatttgc cgggaaaaaa gtaaagcgtg tctctacaag ccgagcaatc
    10081 aaagaaccgc aagtagtaat agacgaagca ttgagagata ttggcgtatt cgttgtgtct
    10141 aatgaaaaag aaccagctgc aaatgagaaa aagccgacaa tctataatca tttcatgaat
    10201 ggtgtgaatt acatgcttcc atttgtgatc gcaggcggaa tcatcatcgc actgagtttt
    10261 gcctttggga ttacagcggc tgatcccaac tcagcagact ataatgtgtt agcagctgca
    10321 ttttcaagga tcggaggaga tacagcgttt gccatgatgg ttcctgcgtt aggcgcgggg
    10381 attgctacat cgattgctgg aaaagcaggt tttgccccag gaattgttgc cggattactg
    10441 gcctctacag gtggttcagg ttttcttggc ggaatgattg gtggcgtgct agcagggtat
    10501 atctgtgatt ttctggcaaa taaaattagc gtaaaaaaag aattttctgc tatttatcag
    10561 ctgattgtcg tccccttttt atcgatctta attattgggc ttttgatggt atttgtgatt
    10621 gaacaaccga tcacatgggt gttggatgct ttgacaaatt ggctgaatag cttgggaaat
    10681 acatccgggc tattgttcgg attacttatc gggatcatga tggcagctga tatgggtggg
    10741 ccgatcaata aatcgacttc gactttctcg atcggtttga tgtcagctgg agtaaatgca
    10801 ccgattgctg cttgtatggc tgcaggaatg acacctcccc taggtattgc tttagccact
    10861 ttattattca aacataaatt taccaaagaa gaacggactt caggtcaatc atgctggatt
    10921 ttaggcgctt cgtatattac agaaggtgct ataccgtttg cggtatctga cccgatccgc
    10981 gtgataccta gtttgatgac aggttcagca gtcgctgcag ggatttccat ggcagtagga
    11041 gttacttcaa tggcacctca tggagggatt tgggtgatgc taattccaaa tgttatgaac
    11101 aatttaccta tgtatgtctt ggcaattgca tcaggtacag tcactacagc ctttatgctt
    11161 ggactattga aaaaaacagt gaaagagaaa aaacaagaca agtggacgaa aaatgaaaat
    11221 ctaacaaatg aggtggaagc gtaatgttat atacaatgaa agagttattg aaagtggcta
    11281 aagagaatca atttgcagta ccagccttca atatttgcag ctatgatatg atgaaagcaa
    11341 tcatcgaaga agcagagaga ttagaagcac cagtaatctt agaaattcat cctgatgaaa
    11401 tcgcctattt aagtgatgag tttgttgcgg cagtaagagc ctatgcttat acaagtaaac
    11461 tgccgatcgt tatccacttg gatcatggcg gtacgataga agatgtctta cgggcgattc
    11521 gaaacggcta ttcttccgtg atgatcgatg cgtctttgaa atcattcgac gacaatgtac
    11581 agattacgaa aaaagtggtt gcgttagctc atgcggtaaa tgtctcagtg gaggctgagc
    11641 ttggaacgat tgggaataat ggttctgctg aaggaggtgc tgaaacaatc atttatacag
    11701 atcctgatca agcgaagatt tttgtgaaag aaacaggaat cgatacactt gctgtagcaa
    11761 ttggaacagc gcatggcttg tatccgaaaa acagaactcc aaaattaaat atcgacttac
    11821 tgaaagaatt gaaccaacaa atcgatcttc catttgtttt acatggcggt tcaggtaatc
    11881 cggacgcaga agtcagcgaa gcagtaaaat acggagttgc caaagtgaat ttgagctcag
    11941 atcttaaaag cgtctttttc aatgctttga gagaaatatt gagtgaaaat ccatcgatgt
    12001 acgagccgaa tgcaatttat ccagcagcta ataaaaaggt acaagaagtg gtcaaacata
    12061 aaatggaaat tttgaacaca gtaggcaagg ctagtttata ctaaaaataa aaatgtgtgg
    12121 tcaagagggt atgacaatag tcttgtcata ccctcttaac cacacacgat gttcagaagt
    12181 agttttttcg aaaaatttct tcttatttct cgaagccaat cacttctgtc acaacctctt
    12241 ttttcctgtt actatataaa gtaaatgggg tgattttatg ttgaatgaag tggagaatag
    12301 acagcaggag attgtgaatt atctaaaaaa caaccaattt gcaagagtca ccgatttgat
    12361 cgagttagta aattatagcg aagccacgat taaacgtgac cttgtttttc tggaaaaaag
    12421 tggattgatc cgtagagttc gcggtggcgc catgcttatt gacaatcgaa aaattgacgt
    12481 gccttacctg atgaagatca ccaaattaga agaagaaaca gaaaaacgta agattgcaga
    12541 tacagcttct tctttgattc gagatgatat ggtcttattc ttggattcca gtacaacctc
    12601 tttacactta gtcagaaacc tagcaagatt tgatggtttg cagatcatta cgaatggcat
    12661 cgtcacagct gctatgttga gtgaatatac aacggcaaaa gtatctgtat tgggaggggc
    12721 aattgttccg aaacgagcaa caatcaacgg ttcaaaagcc tataacgatg cattgtcata
    12781 ttatgcggat ttagcgataa tgagttgtcg cgggctagat ttcagccaag gagtaacaga
    12841 aactcatgaa ggagaggcaa tggtcaaaaa agcttttaga aaacaatcag accggcttgc
    12901 cgttttagtt acacagcaaa aaattgcaca taaattcatg catcaagcca ttgcttttca
    12961 tgaaatcgat tatctggtga caagtaaaga actgacaaaa gaacagtatg cgaaagtaaa
    13021 ggaacaccat atcgaatgtc tgtacggata aattattacg tgaatgttct aagtggcttc
    13081 ctcattttta gataggacct tatctttgct cgcttctctc agccgatggt ataataatga
    13141 aaaggttatc ttgttagata atcttacttt tttatccagg agaaaggagt gcagaaaatg
    13201 aaacatgtga aggcgttggt agtgaaggca atcatgatct gggcgattct gtgggtcgtg
    13261 ctgacaggtt tatacggtgt tagctttatg gattcaacga ttgttggggt aattattgta
    13321 gtcatgatct atgtgctagg ggatctgttg atcttacgta aagtagggaa catcgcagcg
    13381 acgatcgcag atgctggttc agctgcagtt atcttgtggc tctatctata ttttatgaat
    13441 gatacagtcg atatttggat gaaagtattg attcctgctc tattgatcgg tgtggcagaa
    13501 tggttcttcc acaagtggtt gctaagtcaa ggcatcgtac cagatgaacg gaaaatggaa
    13561 taaatcaaca gttaaactat tcttatcgat aaatgaaagc tttaaaaata aatagatgtt
    13621 taattaagaa taatcataaa ctatttttac tcaaaaaacc gctcagatta ttttcagatc
    13681 tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg attcaatcaa
    13741 ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg ctgtacatat
    13801 caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag attttagcat
    13861 ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt aggtagataa
    13921 agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc catttgacat
    13981 aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca cactattcgt
    14041 cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat acatcacgta
    14101 ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg tgatgaccaa
    14161 agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt ttgtataaga
    14221 agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa ttcctgtaat
    14281 gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag atattttttg
    14341 ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg taacaaagaa
    14401 gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca gcaaaatcat
    14461 tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat aattgatgcc
    14521 ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag ctgtagaaac
    14581 tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga tagttggcgt
    14641 ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta aggtaaactt
    14701 ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg ttccgatgat
    14761 atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt cttgcgcaat
    14821 gactgccatg atgaccatca acagataata aatcaggtag acaataaaat tgatcaatgt
    14881 gattccaata aaatatttat taaataattt cttttccatg tatgttatac attcccctta
    14941 agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat tggatggata
    15001 gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat ttaaaaattc
    15061 ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg agttttgata
    15121 cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat atgctgtttc
    15181 taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta ttcttaaaaa
    15241 attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa gtgatggaaa
    15301 aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta aaaagtttta
    15361 aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt ggatagtaga
    15421 gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg atatcataaa
    15481 tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga gggaacggta
    15541 aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat caaagatctt
    15601 gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc gtataatatt
    15661 cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt ttggcaaaat
    15721 atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta tcgtctgctt
    15781 tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc tgtttgtacg
    15841 caactttacg aattatatga tttattcggt gtgcggacag aagaagggat gctgatcgat
    15901 ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc tagcagtgta
    15961 aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga ccatcatatc
    16021 gttattttga atatggactt gttagaagac aatattatct tttgatgaac aaagaggttg
    16081 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    16141 ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta ccaagaggtt
    16201 gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag cttttcatgt
    16261 ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt taccaagagg
    16321 ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac agtctcttat
    16381 gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc gtttatttgc
    16441 aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac attcatttag
    16501 caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag aatgggtagt
    16561 acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg tcgtgacaaa
    16621 agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt ctcggaatgg
    16681 atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat aaaagacaag
    16741 actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca aaaccttata
    16801 gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa ctccgtcttc
    16861 cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg cagcatttgt
    16921 catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca aataatcgat
    16981 catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata gcaatactga
    17041 gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt ctcttggttc
    17101 ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt cattcagctt
    17161 ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag cctcatcatt
    17221 taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta gcaaaagaaa
    17281 gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag gatcgctagc
    17341 ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg caggacaccg
    17401 ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact tgagtcattg
    17461 cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc gtttgtgatt
    17521 gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc ttttaacagc
    17581 ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc atattcgata
    17641 tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat tgctttatct
    17701 aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga taaggcattt
    17761 tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg cttcgtttct
    17821 gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc ctgcatggca
    17881 ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa cttatcgatt
    17941 tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca agcgagaaat
    18001 ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg caagaattct
    18061 ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata agtcgcagca
    18121 gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag atctatcgct
    18181 gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc gtcaaccgct
    18241 ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt aaaatcttcg
    18301 gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt ttgcttttgc
    18361 tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca atgaaatttt
    18421 tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg tgtaagaaca
    18481 tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt aaacggttta
    18541 ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc attgcattca
    18601 ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc tccgcagttg
    18661 gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt atccgcattt
    18721 ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc ttggttattt
    18781 tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac acccataaag
    18841 ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc aatggaacct
    18901 tgcacgtagc cttcacgtac gctaggatca cctagcatcg ccccttcgtt acgcatattg
    18961 atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga aacaatctcc
    19021 atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt gcttaattcc
    19081 aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg attgattgct
    19141 gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt gatttccact
    19201 tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac gacatgaata
    19261 gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc aaagtagttc
    19321 aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat ataaaaaact
    19381 tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc agcaattgct
    19441 tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt gtaataacct
    19501 tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat catgtttgga
    19561 tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg attggagcca
    19621 cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc actcatattg
    19681 tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat acttgttgct
    19741 gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa acaattattg
    19801 tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg aattgaaatg
    19861 gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt ttttttacct
    19921 aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct tatggcatat
    19981 caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg acaaaaagct
    20041 gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc ctacttgttt
    20101 acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa agcaatcaac
    20161 tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg atcaattact
    20221 gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg agtcgaagaa
    20281 atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta caaagtgtac
    20341 attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt gaaaacattg
    20401 gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca caaaatacca
    20461 gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac ggcagatatt
    20521 gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc agctgcattg
    20581 aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat tcttgatcag
    20641 gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt gacaggtagc
    20701 ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga agtaccagaa
    20761 gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg ctttctagag
    20821 gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa cctagttgag
    20881 gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt ttcagcggat
    20941 cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg ttttaccaat
    21001 agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc gtctgcttct
    21061 gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct acaacaagag
    21121 attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat tgagaataca
    21181 aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta tcgtgtaccg
    21241 acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa ccaagtaaaa
    21301 gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat gcttaaagca
    21361 agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta tgagattgtt
    21421 tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa cttaagccga
    21481 atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg gccagaactt
    21541 cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt tgcttctgaa
    21601 gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga agaaacactt
    21661 gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga tgattaataa
    21721 aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga tgaaacaagt
    21781 acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa cagagtttac
    21841 aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc gcatgaaaga
    21901 tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc aggatcttgt
    21961 cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga aaaaattagg
    22021 gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa tgaatatgca
    22081 atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag gaatcggtca
    22141 gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag tagtaaatga
    22201 atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta tctgcggaaa
    22261 catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc gaagcgttat
    22321 cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag aatatcacgg
    22381 actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc cagaagacat
    22441 caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag tgattatcgc
    22501 aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc tgatcaaacc
    22561 cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg atatcgagta
    22621 tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat aaatttctaa
    22681 ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca acaatgtgaa
    22741 cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca tcttgaatga
    22801 actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa gagaaccagg
    22861 aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa atgatcggat
    22921 ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc tagtcgaaaa
    22981 ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg tcgacagttc
    23041 tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc ggttgaatga
    23101 gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag attcagacac
    23161 cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag attcggaagg
    23221 tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag agaatccgga
    23281 aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa caagttatca
    23341 tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac gtttatgaaa
    23401 attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga attgattgat
    23461 gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc tggaaacagt
    23521 acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat catcaaaaaa
    23581 acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat ttcgatggat
    23641 gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt cgtcttacct
    23701 gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa taaacttggc
    23761 tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg ggcgtatcgc
    23821 tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag gtatatggct
    23881 tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa aatgcaacaa
    23941 ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac cggaaggaca
    24001 aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca gcggttacga
    24061 atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca gtgcggcaaa
    24121 cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt tagaaacaga
    24181 tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc attttcaaga
    24241 actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat tggaaaaagc
    24301 aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct ctcaagatga
    24361 atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc aaaagcaaga
    24421 tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag aaaaaacaca
    24481 acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc gtcaagcagt
    24541 agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac ggatattgat
    24601 cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac agttcgtttt
    24661 gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga aatggtagaa
    24721 gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc aggagactct
    24781 gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca tattgctact
    24841 gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat ccgtccgatc
    24901 ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa agatgcttat
    24961 gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat gaaaatggtc
    25021 aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc ggacgggcga
    25081 attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg tattgagctt
    25141 cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc atgtgggcgt
    25201 ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa aatggcaaaa
    25261 gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg cttgatgtgc
    25321 tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc tgactttgga
    25381 aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct gcttagccgt
    25441 atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg ggatgaattg
    25501 aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg acggattgaa
    25561 tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga agcatgcttt
    25621 gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc tgcgggaaca
    25681 cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc aggaactttc
    25741 taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct gctacgattt
    25801 atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag tgatctatcg
    25861 cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac tgctccagtc
    25921 tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg aaatccggta
    25981 tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat gagctacaga
    26041 agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg taatacacaa
    26101 aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga acataattat
    26161 aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat tgctcaagtc
    26221 agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt agcctgcata
    26281 aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac ggctttgatt
    26341 gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg taagaaaaaa
    26401 gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat tttttatgag
    26461 tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg aaatcgccaa
    26521 gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg aagtacggaa
    26581 gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct gcttgtagaa
    26641 gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac gttaaaggaa
    26701 caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa agcagtagcg
    26761 ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt agactgagat
    26821 agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact tttcaaatct
    26881 cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat ggagctgcaa
    26941 tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga tgcttttttc
    27001 gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt aattgccaga
    27061 caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga agcaagaaaa
    27121 ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc gcaagccatc
    27181 tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg ggaagtattc
    27241 tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct tgatgtgacc
    27301 gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca agcagatatt
    27361 tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt tttggccaaa
    27421 ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca agacgctgtg
    27481 ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa aactgtacct
    27541 agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac agaaatgatg
    27601 ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag aggaatccat
    27661 gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca tacttatggg
    27721 cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc tgaaaaagta
    27781 gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa ggtcaggtac
    27841 acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta taaaaaagaa
    27901 gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga aaaaggaatc
    27961 cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa catcgtcctt
    28021 ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc ggaaatccgt
    28081 tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc ataggtttct
    28141 gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga tttttggacg
    28201 tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg tttgcttaat
    28261 tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt atcttctgaa
    28321 tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt tgtcgtaccg
    28381 aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa aaaaggttcg
    28441 ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat gatttgttct
    28501 tctaattcag gtgtgaatgg tttgccatat cgaaaatttt gtgcagcttt gttgaagcag
    28561 ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc ttcaccatcg
    28621 acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt tgctaaccat
    28681 tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca aagatatagc
    28741 tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta aatccattgt
    28801 tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc catcgaaaga
    28861 gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt tcaaaagctt
    28921 gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt tgtttagcta
    28981 ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt ttcacacgtg
    29041 aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt cctaaatagc
    29101 cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat tctggacatt
    29161 cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag caatgatcga
    29221 ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat acagcttcta
    29281 aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat ccacctgaac
    29341 aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg tttttacgca
    29401 catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg atgatgccga
    29461 atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga gttgaataga
    29521 cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt aattctttga
    29581 cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca ccgaagaaag
    29641 ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga cggtttttga
    29701 ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca tacatatata
    29761 aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag agaagcgcat
    29821 ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga tctccttttg
    29881 attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt cgtccgacat
    29941 ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag cccataggta
    30001 ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca ggatacattc
    30061 ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct tcagggttta
    30121 agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc cgcttttcac
    30181 tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt tcaaaccagt
    30241 tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa gtaaataagg
    30301 tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg gtttttgctt
    30361 ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct tttagatgtt
    30421 ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg gcagaacagc
    30481 caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct gtggcggtct
    30541 ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg ttcagcatgc
    30601 ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa tccaaatcat
    30661 ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac attttcagtc
    30721 cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa acattactgt
    30781 ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt tcaatcgaaa
    30841 agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa ttgatgtact
    30901 cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg ttttgaatct
    30961 catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc aagtctgcaa
    31021 tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc ttttctgctt
    31081 tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca tcgcgtttga
    31141 tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct tcataattta
    31201 ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca aaatcgggga
    31261 aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt ggatttctat
    31321 actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct ggtggatttg
    31381 ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt attcagtcca
    31441 agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta tgcaaataaa
    31501 tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa gtgaaacatc
    31561 taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat atctcattca
    31621 cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga aagacaacgc
    31681 tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg atccaaggaa
    31741 aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa aaactaggat
    31801 tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat atcctattgc
    31861 ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga catgtagctc
    31921 aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct gtcggacaga
    31981 gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt tttgcagatg
    32041 aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac ttgaagacca
    32101 tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat acagcaagct
    32161 actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt gtcagaagag
    32221 ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt ggcggaggag
    32281 gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc acgaggctat
    32341 cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt cagcgcaatg
    32401 acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga tggtggattg
    32461 agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag tgccaaccga
    32521 tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg tatgaataaa
    32581 ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt cgcctgtatt
    32641 tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt ggttcgaatg
    32701 atggatatgg atcttactag tccatttttt atctccattc catcagtagt agatacttta
    32761 tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa aatatggcgc
    32821 tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac aatgcgcttc
    32881 aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag cggatatttt
    32941 ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta tcttgataca
    33001 ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac ttatttgttt
    33061 ttctgttata catttcggtt atttttgatc atattgagta aatggaagat ttattactat
    33121 ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag ttggcgtaca
    33181 aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg cacggctagt
    33241 atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta tcagtttgat
    33301 gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa gataactaaa
    33361 gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct cgaagaacct
    33421 atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag aaaagttaat
    33481 ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct tgatcaaatg
    33541 caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc agataatcaa
    33601 gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat ggtatatcta
    33661 agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt ggattatcaa
    33721 gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg actgagcaat
    33781 gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt aaatggaagt
    33841 ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga agcacaattt
    33901 agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg tgcagttaat
    33961 cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt gattattgct
    34021 actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa agaaaattat
    34081 cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat cttcgagcaa
    34141 aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt atttgcaatc
    34201 aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct tgtgagtggc
    34261 ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata ttgtcggatt
    34321 atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt ttgaatacca
    34381 tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat tttttatatt
    34441 ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct atttttataa
    34501 aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac acatgtttct
    34561 ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc caatgaacaa
    34621 atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa caatttttta
    34681 tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc aaggaggaac
    34741 ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt attgaagcaa
    34801 tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat gagacgcata
    34861 cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa aaaaaggtag
    34921 aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt gtctcatttc
    34981 taggggttgt caaatctggc catgcctatt tgccgattga agagcataca cccaaggagc
    35041 gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc ggggactgga
    35101 tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa atcaagaaga
    35161 taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt tatattattt
    35221 ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat aatctagtca
    35281 gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat ttcttagccc
    35341 aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta gtttctggcg
    35401 gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta ttcgcgacat
    35461 tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat atttgcttga
    35521 tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta ttttgcggag
    35581 aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat gcacggatat
    35641 ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag attgaccagc
    35701 atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac acgaaagttt
    35761 ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc attattgcag
    35821 gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct gcgttctttt
    35881 gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat gaatatgggc
    35941 ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac cggattgaac
    36001 tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct gtcgtcgtac
    36061 caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta gaaaacaaaa
    36121 cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta gaagttgtaa
    36181 tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag acagttaatg
    36241 gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt tacctcatat
    36301 gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg taccgatcgt
    36361 tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag tgacactttt
    36421 ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc tgattgggta
    36481 tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa ggaaaaatca
    36541 gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt taacgaaagt
    36601 gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa tctcttattt
    36661 aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca aaacatataa
    36721 gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg gaccaatcga
    36781 tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata aatatgttga
    36841 tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat tcattatcgg
    36901 ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac agcatggtgg
    36961 tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt tttttgattt
    37021 tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc agacaccaat
    37081 caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc gttggcatat
    37141 gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct tcatgatgaa
    37201 gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg ctttgtttct
    37261 tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac tgtatcatgc
    37321 ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata aagagcagtt
    37381 gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag cagtatgttt
    37441 tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac gaatagtgac
    37501 atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt atcaatctta
    37561 gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct atttgatgaa
    37621 gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg agagctggat
    37681 atccaagtcc ctgtatctga attcgagcga gaggattggt gcacaccgaa taaaatcatt
    37741 caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct attttcggcc
    37801 cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat caagtagata
    37861 tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac gtgttgaaag
    37921 gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt tttggctcaa
    37981 gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa taccagcgaa
    38041 actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa tatatgatga
    38101 tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt tctcctcagt
    38161 ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct gaacttcaaa
    38221 catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat ttagctagaa
    38281 gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt ctgcaaacga
    38341 tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc caatggaaca
    38401 tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga cagcaaaaaa
    38461 tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta tccattttag
    38521 caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa aacgattttt
    38581 atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa aattgggatt
    38641 atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta gcaaaaaacc
    38701 ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat tataccggac
    38761 tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta aatagccaag
    38821 gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt atggaagaca
    38881 cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga ccgtttttag
    38941 aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt agcaaatctt
    39001 ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg agtgttgctc
    39061 gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa gtttgcctga
    39121 acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg gaaaacattt
    39181 ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag tacctggaaa
    39241 tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg atgatatttt
    39301 ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc ccaaggtctt
    39361 tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg aaaaaggatt
    39421 cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc agcaagttga
    39481 aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac caccacatgt
    39541 tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca ttgatattgg
    39601 cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata tggtcactcc
    39661 atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag catgggataa
    39721 tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag aagcacaccc
    39781 aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag gtgctcgaac
    39841 aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta cagcaggaga
    39901 cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc cacagatttt
    39961 gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta ttgatgtagc
    40021 agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa ctgatgcagg
    40081 acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc ctgttcgtta
    40141 tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag tgaaactagt
    40201 cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga gttatgaata
    40261 atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc tttggaaaat
    40321 caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag cgtcatccag
    40381 aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca cttattgagt
    40441 tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta cattgcgcta
    40501 gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt ccaaagaaag
    40561 tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg gctactcaac
    40621 agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat gcagctgctt
    40681 ttcagttcaa atgcggtacc tacgggtgtc atgccagaag attttcttga tatcagcttt
    40741 gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat tgttggcggt
    40801 ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt tattcaatgt
    40861 ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt gttgcagctg
    40921 tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct tttgtgggtc
    40981 attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat tgaaaaagga
    41041 ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa aataactttt
    41101 gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt aggatgctta
    41161 ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc tgacgggttc
    41221 aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca ccttgatcca
    41281 cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc tgaccttggt
    41341 gtattacagt attactggca acttcatcct aagagaaatc cggagacatt gctttcaatt
    41401 tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa tgtctcaaaa
    41461 ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat tacttttatg
    41521 gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac aacggggaaa
    41581 gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt tgcgaaagat
    41641 gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca tcatgcccat
    41701 ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa acttgtactt
    41761 acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca aaaggcagca
    41821 ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat ttaggaggaa
    41881 gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag caattgaaca
    41941 atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata tcaatgaacc
    42001 ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag attatgtgga
    42061 agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct accatgattt
    42121 tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc gtgactttgc
    42181 gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg gggcggaatt
    42241 gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg cgatgtatgc
    42301 taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata tcaatgaagt
    42361 agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca aatcgatccg
    42421 ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa tgacaacaga
    42481 agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag aagaagtaaa
    42541 aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg aggaatatta
    42601 tcgaaattgg gattggaatt acgggaagtc tccagatttc aatttctctc gccaaaaacg
    42661 tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga tcaaagaagc
    42721 aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa aaaaattggt
    42781 tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg atgtgaagaa
    42841 atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa aataaaaaat
    42901 agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt aatttcgaga
    42961 gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg ttcctgtgta
    43021 acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat ctaattcttc
    43081 tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc aacaaatgac
    43141 gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg gattttcgat
    43201 ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag ccagatcgtc
    43261 atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa tattccgttt
    43321 ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt tgcaaaaaga
    43381 acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt ttttagcttt
    43441 tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa attccaggcg
    43501 aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct ccaacaaatc
    43561 atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta agatgtgatc
    43621 gattagattg tagctgataa taacgattag aatgataata tataaaatcg tagaccaatt
    43681 gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat ggataatggc
    43741 taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat atgcttcgcc
    43801 aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag caatcggcat
    43861 taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga taaaaatcaa
    43921 aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc ggctccaata
    43981 gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa aagaatgaac
    44041 gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa atcctaatgc
    44101 caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt tatacatcaa
    44161 gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca tcgtcaatac
    44221 ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc ctagataaac
    44281 tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg tcaatttttt
    44341 tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat tgctttcttc
    44401 ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa tccattgatg
    44461 gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg tttccataat
    44521 gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt caaacttaaa
    44581 atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca tttgattgct
    44641 gtccgcagga gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa ataacaatga
    44701 taatataatg attatgctcc actttatacg taaataaaaa gattcttttt tattatatat
    44761 gttattaaaa aaagacataa aggtattcct ttctaaaact tgtaatgtta gggtaaacgt
    44821 tttttaaact aacgtcaatt atttataata aaaaatgaga taaagatgag gaaatagaat
    44881 tagaaacata taattattaa agggaaacat ttttacagct ataatttgaa aaaatttatc
    44941 gttattatat gattaagaag gattgaataa agaaagaagt tgaaaacgat acctgattac
    45001 aaaaaaataa cttttataag ttctataacg ttgattatat ttttaattct tattcttctc
    45061 gtcgtcttgg ttccccgaat ttttggtttg actccttata ttattgagga caaagaaatg
    45121 agtccatagt atgcacaagg aaatctgata tatgtacgac cgaaaaatac aaatgagatt
    45181 ttagtcggag atattattac atattatgaa aattcaggaa aaaaaatctc gacgcgtcgc
    45241 gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac aaagacttat
    45301 attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt tcagattcca
    45361 tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt attctttacg
    45421 attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt gaaaaagaag
    45481 cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat tccagagatc
    45541 caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta gatttagtca
    45601 gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga tgcatcgtcc
    45661 tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat taccgggtga
    45721 gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc cccatgaaac
    45781 agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct tggagatcgg
    45841 agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa acgctcatgt
    45901 tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact atgaacgtct
    45961 aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct tgccggagtt
    46021 gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata ttgaattttt
    46081 acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg acatcttcca
    46141 aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag ctatccatcg
    46201 taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat cgacaatctt
    46261 gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg agttgtgata
    46321 catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg aacttgatgt
    46381 aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata aacaggagta
    46441 gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct gaaggagaat
    46501 gtctcgtttt aaaagat
//
LOCUS       DAYYGS010000012        44822 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000012 DAYYGS010000000
VERSION     DAYYGS010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.012"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..1012)
                     /locus_tag="ACHA44_000628"
     CDS             complement(2..1012)
                     /locus_tag="ACHA44_000628"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8903944.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1192..1536)
                     /locus_tag="ACHA44_000629"
     CDS             complement(1192..1536)
                     /locus_tag="ACHA44_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HFM8903945.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1529..1963)
                     /locus_tag="ACHA44_000630"
     CDS             complement(1529..1963)
                     /locus_tag="ACHA44_000630"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFM8903946.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2101..2838
                     /locus_tag="ACHA44_000631"
     CDS             2101..2838
                     /locus_tag="ACHA44_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8903947.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2831..4042
                     /locus_tag="ACHA44_000632"
     CDS             2831..4042
                     /locus_tag="ACHA44_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8903948.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4246..5028
                     /locus_tag="ACHA44_000633"
     CDS             4246..5028
                     /locus_tag="ACHA44_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HFM8903949.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5098..5760
                     /gene="trmB"
                     /locus_tag="ACHA44_000634"
     CDS             5098..5760
                     /gene="trmB"
                     /locus_tag="ACHA44_000634"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFM8903950.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHA44_000635"
     CDS             complement(5858..7612)
                     /gene="ade"
                     /locus_tag="ACHA44_000635"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFM8903951.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7744..8673
                     /gene="comGA"
                     /locus_tag="ACHA44_000636"
     CDS             7744..8673
                     /gene="comGA"
                     /locus_tag="ACHA44_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HFM8903952.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8630..9679
                     /gene="comGB"
                     /locus_tag="ACHA44_000637"
     CDS             8630..9679
                     /gene="comGB"
                     /locus_tag="ACHA44_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HFM8903953.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9676..9954
                     /gene="comGC"
                     /locus_tag="ACHA44_000638"
     CDS             9676..9954
                     /gene="comGC"
                     /locus_tag="ACHA44_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus major pilin ComGC"
                     /protein_id="HFM8903954.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9929..10399
                     /gene="comGD"
                     /locus_tag="ACHA44_000639"
     CDS             9929..10399
                     /gene="comGD"
                     /locus_tag="ACHA44_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HFM8903955.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10371..10718
                     /locus_tag="ACHA44_000640"
     CDS             10371..10718
                     /locus_tag="ACHA44_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903956.1"
                     /translation="MVAIQKELNKTGFVLLESLTALMIASVCVYLVSFGQLFLLQAEK
                     KSGEELLFIRMIYEDIKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAI
                     SIEKTSNSSIYAD"
     gene            10681..11106
                     /gene="comGF"
                     /locus_tag="ACHA44_000641"
     CDS             10681..11106
                     /gene="comGF"
                     /locus_tag="ACHA44_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HFM8903957.1"
                     /translation="MKKQVIPAFTLIECLIGLMILSSLLLTFNLLIQQTIQVSEFLQR
                     KDQKEWLIFLAQLEEELKSSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQ
                     PMLTSITELQLLEKEHTISFYVHFENGEDGYAIWTKNDQ"
     gene            11078..11437
                     /gene="comGG"
                     /locus_tag="ACHA44_000642"
     CDS             11078..11437
                     /gene="comGG"
                     /locus_tag="ACHA44_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HFM8903958.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11609..12616
                     /locus_tag="ACHA44_000643"
     CDS             11609..12616
                     /locus_tag="ACHA44_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8903959.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12641..13825
                     /locus_tag="ACHA44_000644"
     CDS             12641..13825
                     /locus_tag="ACHA44_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HFM8903960.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHA44_000645"
     CDS             complement(14262..15845)
                     /gene="abc-f"
                     /locus_tag="ACHA44_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protection-like ABC-F family protein"
                     /protein_id="HFM8903961.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIVLVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(15950..16078)
                     /locus_tag="ACHA44_000646"
     CDS             complement(15950..16078)
                     /locus_tag="ACHA44_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903962.1"
                     /translation="MMRMTKITSNENYQRYHIAFSHAIGFTVAVDGTSLSKTVEFT"
     gene            complement(16423..16566)
                     /locus_tag="ACHA44_000647"
     CDS             complement(16423..16566)
                     /locus_tag="ACHA44_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFM8903963.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16789..17268)
                     /locus_tag="ACHA44_000648"
     CDS             complement(16789..17268)
                     /locus_tag="ACHA44_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8903964.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17341..17625)
                     /locus_tag="ACHA44_000649"
     CDS             complement(17341..17625)
                     /locus_tag="ACHA44_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8903965.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17703..17897)
                     /gene="ssrS"
                     /locus_tag="ACHA44_000650"
     ncRNA           complement(17703..17897)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHA44_000650"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17969..19255)
                     /locus_tag="ACHA44_000651"
     CDS             complement(17969..19255)
                     /locus_tag="ACHA44_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFM8903966.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19393..20064
                     /locus_tag="ACHA44_000652"
     CDS             19393..20064
                     /locus_tag="ACHA44_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HFM8903967.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20030..20518
                     /locus_tag="ACHA44_000653"
     CDS             20030..20518
                     /locus_tag="ACHA44_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HFM8903968.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20531..21478
                     /gene="prmA"
                     /locus_tag="ACHA44_000654"
     CDS             20531..21478
                     /gene="prmA"
                     /locus_tag="ACHA44_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFM8903969.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21479..22243
                     /locus_tag="ACHA44_000655"
     CDS             21479..22243
                     /locus_tag="ACHA44_000655"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFM8903970.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22476..24689
                     /locus_tag="ACHA44_000656"
     CDS             22476..24689
                     /locus_tag="ACHA44_000656"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFM8903971.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24700..25146
                     /gene="dtd"
                     /locus_tag="ACHA44_000657"
     CDS             24700..25146
                     /gene="dtd"
                     /locus_tag="ACHA44_000657"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFM8903972.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25317..26576
                     /locus_tag="ACHA44_000658"
     CDS             25317..26576
                     /locus_tag="ACHA44_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HFM8903973.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26665..27612)
                     /locus_tag="ACHA44_000659"
     CDS             complement(26665..27612)
                     /locus_tag="ACHA44_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8903974.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28006..29298
                     /gene="hisS"
                     /locus_tag="ACHA44_000660"
     CDS             28006..29298
                     /gene="hisS"
                     /locus_tag="ACHA44_000660"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFM8903975.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29313..31082
                     /gene="aspS"
                     /locus_tag="ACHA44_000661"
     CDS             29313..31082
                     /gene="aspS"
                     /locus_tag="ACHA44_000661"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFM8903976.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31191..32066
                     /locus_tag="ACHA44_000662"
     CDS             31191..32066
                     /locus_tag="ACHA44_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8903977.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32332..34215
                     /locus_tag="ACHA44_000663"
     CDS             32332..34215
                     /locus_tag="ACHA44_000663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8903978.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34290..34574)
                     /locus_tag="ACHA44_000664"
     CDS             complement(34290..34574)
                     /locus_tag="ACHA44_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFM8903979.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34564..35298)
                     /locus_tag="ACHA44_000665"
     CDS             complement(34564..35298)
                     /locus_tag="ACHA44_000665"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HFM8903980.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35427..36050
                     /locus_tag="ACHA44_000666"
     CDS             35427..36050
                     /locus_tag="ACHA44_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFM8903981.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36242..37363
                     /locus_tag="ACHA44_000667"
     CDS             36242..37363
                     /locus_tag="ACHA44_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8903982.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37370..40498
                     /locus_tag="ACHA44_000668"
     CDS             37370..40498
                     /locus_tag="ACHA44_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8903983.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40517..43711
                     /locus_tag="ACHA44_000669"
     CDS             40517..43711
                     /locus_tag="ACHA44_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HFM8903984.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43857..44564)
                     /locus_tag="ACHA44_000670"
     CDS             complement(43857..44564)
                     /locus_tag="ACHA44_000670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8903985.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        14382 a         7961 c         9405 g        13074 t
ORIGIN      
        1 tttattttga tgaatcagtt gtttcttcag ttgaagattc ttttgttgaa tcagttgatt
       61 ttgtgtcaga actctttgtt gaagctgtag tttcacttga gtctttcgta cttgatgaag
      121 ttgttgtgaa tgcactcaac acattttcaa aggcatcatc tttgattttt acatttgcat
      181 cttttaattc ttcaccgatt actttagttg tgaatgcatt gtcacttagt ttagtttcag
      241 tagcgatatc ttttagttga tctttgtatt tatccatgtc attgccttta ttttggtttt
      301 tcaccatttt gacaacgtag tattctgtcg cataagaagt aggatttgtt gtagtaataa
      361 catcggagat ctcaccgtct ttcaatttga aagctgcttc ttttacttct gcaggaatag
      421 ttgttgtcgt tgagtcgaat ttaactttac cgccatcttc ttttgtctct gtatcagttg
      481 atttgtcttt ggctaattta gagaaatcat ctccatcatc cgctgatttt ttcacatctt
      541 ttgcttcatc ttcacttgat aatttgatga tttgtgcttc tacttctgga tggaaagatt
      601 tccaagcagt tttcaaatcg tcatctgtaa tatctacgtg agctttcaag ccagcttcaa
      661 aagctaaatt gttacgaata tattctttat aagtatcttt cgtatatcca gcagcttcta
      721 attgtgattc aaaagtatca cctaatgatt tcgcttgttt gtcgtattct gcatccactt
      781 gcttatcggt tactttatcg ccatatttgt tattgaatac tttataaatg atcatccgtt
      841 gtactaagga ttggttcgat gattcaagtt ttgcttcatc atagaaatca gatacagtga
      901 tcgttccgcc tttcatcgtt gcgatatctt tattagtatc gcctgaacaa gctgctagag
      961 ttaaaacagc caacgcacta gtagctgcca aaataattga tttttttttc attaaaattt
     1021 gcactcctgt aattagttat tatttttata tattaactat accatatccc atttccttta
     1081 aaaacgttgt cgtggtagtt tcaaacaaaa ttcaaaattt catcacattc ctaggaaata
     1141 cgcaaacttt tctcacttat atagttaaat ctgttgagaa aataatcatt attatatttg
     1201 ttcaggatac tctggcagat cagcttttag tttttctact tgttcattga tttgtgtgat
     1261 acgtggttcg gcttggaatt taaaatcttc gatatctttt ttgattgctt cttggaaagc
     1321 aggaatcaca gattcagctg tttcttttgt ttcgatcaat gcgcttttaa aatgattcag
     1381 gctgttattt aaatcatttg tcagagttgt tgcttcttcc aattcttcga tcagtttctt
     1441 tcttgtttca ttcccgctcc tgggcgccaa taacagacct ccgacagtgc cagcagcagc
     1501 tccaaataac agtcctttga aaaagttttt aatcatcgca ggacacctgc tttacgattg
     1561 tttcttgaat agcttttagt tcttccgatc cataactatc ttgatgattg acaaagtgaa
     1621 tagaaaaatc atcctctttg ctgtagcgag ggactaaatg gatatgtgaa tgaaaaacag
     1681 attgataggc tagttcacgg ttgttattca agatattaag acctttcatt tctggaaaag
     1741 ctttttccaa tgcttgagca atcttaggca cacgcgaaaa aacctctcct gcaagttcgg
     1801 gatcatattc aaaaatatct gctacatgac gtttaggcac aactaatgtg tgtcctttcg
     1861 ttgcttgaga gatgtctaaa aaagcataga ccttgtcgtc ttcatatatt ttatagcttg
     1921 ggatttcttg attgatgatt ttgcaaaaaa tacagttttc catataaaac atcctttcta
     1981 taaagaataa atacttcagc ttaactttac cataagttaa agctattgta aggaaaaaaa
     2041 caaaataccg tcattttctt cgtggtataa taaataaaaa taaatggcgg aggaaaaaga
     2101 atgagtttaa ttatcgatca tgtcacaggg gggtacggac atctccctgt gttaaaagat
     2161 atcagtttcg atgtacagcc tggagagata gtagggttga ttggactgaa cggagcaggg
     2221 aaaagtacga ccatcaagca cattatcggt ctgctggaag cttcaaaagg aacgatagaa
     2281 atcaatcatc tatccttaaa gaaaaatacg gaagaatatc gcaaacaaat tgggtatatt
     2341 ccagagactc cttctttata tgaagaattg acattgagag aacatatcga agtcactgcg
     2401 atggcctacg atatacctat ggatatagca atgaaaagag cagaaaagtt gttggagact
     2461 tttcggttat ctaatcgtct tgactggttc ccagctaatt tttcaaaggg aatgaaacag
     2521 aaagtgatga tcttatgcgc ctttttgatc gagccttctt tatatatcat cgacgaacca
     2581 tttttaggct tggatccact agctatcaat gcattgcttg aattgatggt agaaatgaaa
     2641 aaagaaggag ccggtatttt gatgtctacg catattttag ctactgcaga aaaatattgt
     2701 gatcgttttg tggtcttaca taacggagag atacgagcag acggttctct tgagatgttg
     2761 agaaaagaat ttcagctgcc ggattcttct ttggacgaaa tctatattgc tttaacaaag
     2821 gagcaggcac atgagtagtt tttttcaaaa acgtttagca agacatcaaa agaaaatgat
     2881 gaaatatatg cgttatgtat tgaacgatca ttttgtttta gtttgtcttt tcttgattgg
     2941 cggtgtggga ttatattatt ctaattggtt gaaaaccctg cagccaccgt ttgcaattgg
     3001 agggctcata atctctgtat tttggatgct ctgtttattc gtgggtaaga tggctacttt
     3061 tgcagaggca gcagatattg tttttttact tcctaaagaa aaagaaatgc aaacctattt
     3121 ggaacgagct tttcgctatt cttgcatttt tccgttgatc tttttgatat tggcatgcgg
     3181 attggccatg ccattagtgg tggttagtac ggagcaatcc ttttcgatgt ttttcgtcta
     3241 tctgatcata ttatggagtt tgaaattcag tcatctgcaa atcaagagag cagaattgtt
     3301 tcgactagat caacaagtta ttcgttattg gaaattaggc tggttttttt cttctctgat
     3361 ctttttgttt ctatcatttt attggctaat atgggcagga atagcaggtg cattagtaca
     3421 ggcaagtcta tattactggt ttttgtggaa gcaacaagaa aaaggattgg attgggagaa
     3481 aatggtgcat gtagaagaac ataggttgca tcagatctat caatttatca atttattcac
     3541 agatgttcct gaaattacgg caaagatcaa aagaagacgc tatttggatc ctatattgac
     3601 tcatattaaa agagaatcaa aaaacactta tctttacttg tttgcaagaa gattggcaag
     3661 aggttctgat tttagtggtc tttatttccg attagtaatt ataggtgggt tattgattgc
     3721 cggagtagtc gacttttatt ttagtctgat tatcggtttc ttatttttat atttgatcgg
     3781 tttccaactg ttacctttag cgaaccagtt ccgctatatg gttctcgttc agctttatcc
     3841 aatccataag aatcagaaga agcaggcgat tcagtatcta ctcacatggg tattaattat
     3901 cgcgtcggtt atttttggtt tgattgctgc agtcgtatta gatgggcagc agcgatgggt
     3961 tccgctagcg gcttatttga ttgttactat gctctttgtt aaactatatg taccaactcg
     4021 tttgaagaag atgtcagact gaaaacaaag ctgtgtaggg taaaaatgag aatgaaaaat
     4081 agagcgttga cagtaagaat gctgttcgtt ctattttttt tgagaaaagc tggttttttg
     4141 aatggcaatt aaatgaaaaa gaacgtatat gtgcttgacg tgtagtgttc aggcgactag
     4201 aataataagt actgcaaaac aaagagtaag aggacgggag aatgcatgat ggagtttcag
     4261 ttagaccaag agtggcggtt acgtcccatc aaaggcgaca caggaaaaac atacataggc
     4321 atgcgtgatg aggacaaagt ctttattaaa cgaaacacga ctccgatgtt ggctgctctt
     4381 tcaagggaag gaattacacc aaaacttgtt tggacaaaac gaacggggaa tggcgatact
     4441 ttgacagcac aagagtggct ggaaggtcgc ttgctgactc ctaatgagat tggtcaaaga
     4501 aacgacgtga ttgatgtttt gtaccattta catcattcaa attctcttaa aaccatgctg
     4561 acccgtatcg gtggtcgtgt catgacgccg gagaagatgc tgcaaaaata tgaaaaagaa
     4621 ttaccggaag aactccagaa aaatcaattt ctgcaaattg tccatcgttt tcttcgtgag
     4681 cagatcccgt caatagataa ggaaaactta tcagtagtac acggagatgt aaaccatcgt
     4741 aattggttag tatgtaaaaa ttatttatat ctagtcgatt gggattcaat catgtttgca
     4801 gaccctgcgg tggatattgc aacgatttta ggaaattatg tgccactgtc tagctggaat
     4861 cagtggctga tcagttatgg catacgcccg actgacgaaa tgcttgaaaa atttcattgg
     4921 tatgcggcca tgaatttgct ccaagaaatc accagatatt gcaaacgtga agatcatcga
     4981 cggatgaacg aagaaatttt acaattaaaa cgaattttca gtggctgagg tttggcccaa
     5041 actctgcgga gatttggcca gaccttttta attagaggaa aggaatggag ataaataatg
     5101 cgtgtccgaa atcgtccggg agcagcggaa atgttagcgg ctcacccgaa ttttgtaatt
     5161 agtgatccaa cattgtggaa gggaaaatgg aatgaattgt ttgaaaacga tcatccaatc
     5221 cacattgaga ttggtatggg gaaaggccag ttcatcactg gaatggcaaa agcccatcct
     5281 gaaatcaatt atattggagt agaaatgcag gtcagtgttg tttcgattgc actagacaag
     5341 ttgatcgaac aaccgcttcc taacctgaaa cttctacatg tggatggatc agcgttgaca
     5401 gagtatttcg ctgacagcga agtagatcaa atctatctga atttttccga tccatggcca
     5461 aaaaaacgtc atgaaaaacg gcgattgaca tataagactt ttcttgctgt agatgaacag
     5521 attcttcgtc caaatggaga aatccatttc aaaacagata atcaaggatt gtttgagtat
     5581 tctctggcaa gtttttctca atatggcatg atcctcaaac aggtatggtt agatttacat
     5641 caaagtcaat ttgaaggcaa tattatgacg gagtatgaag aaaaattttc ttcaaaaggt
     5701 caacgtattt atcgagtaga agctcaattt caagataaga ccaaaaagaa aagtctgtga
     5761 aactaacttt ctgctttcat aaaaacatta atatccataa ataaatccca gaaaatgaac
     5821 attacagatg taatgtattt tctgggattt atttgattta aaatgtattt atttcatcgg
     5881 tttttccaca aggtggaatc aattggaagc gagaaaatga aaataatcct ttgtcggtga
     5941 tcttcaattc gggtatgaca gataatgtta agaaagaaag tgtcaaaaaa ggatcaaagg
     6001 ttttagctgc tcctagttga tgtgctttag ttattaattc attcaactgt tcatagacga
     6061 tcgaaaaagg ttcctgtgtc atgattccgc ctataggaag agaaacacaa gctatttcct
     6121 gctgattgtt gacaacggtc atcccgccac ctttttgaat caaaagatta gcagcataca
     6181 gcatatcctc gtcgtttgtc ccaacgacaa ctagattatg cgagtcatgg gcaatcgttg
     6241 tcgctatcgc tccttgtttc agctgaaatc ctttcactat gcctaatcca atgtttcccg
     6301 tttgatggtg acgctcgatc accgcaatct ttaattggtc tttttcgatt gaaggaatga
     6361 atcgttcgcc ttgtcggtcc actttttcta ccaaatcttt agtcactaga ctattcggga
     6421 taatttcgat cacatgggct tgtgaagaag aaagaagcag ctctaacgaa tgtggattta
     6481 atggttgagc gtttagtttt ggcagacaac acgtatatga atttgaatga tcagcaaata
     6541 aatggtcttg gagttgccca gattgaacaa ctaatgttcc cttatagaaa acttcatgaa
     6601 ttggcagctt ggtaagatcg tctgttaaga agaaatcagc ttgataccca ggggcaatcg
     6661 cacctagtgt tttcaaatca aaacattctg ctgcattcaa tgttgccatc tgaatggctt
     6721 gtaatggatc gattccaagt gcaatagctt gacggacgat atgatcgatt gatccttctt
     6781 ttactaaatc attgatcagt ttatcatcgg tgacaaagaa gcatcttctt gaattttcag
     6841 gtgtgatcgc cggtagtagc gatgccagat cttttgcaac ggtcccttct cgaaccatta
     6901 aatacatacc aagatccaaa cgttctttag cttcttttgc agttgtagct tcatgatccg
     6961 tccgaatacc tgctgcagga taaacattta actcttccat gccgatacca gctgcatgcc
     7021 cgtcaatttt tttcttatgc tggtgcatca attggatctt ttctaggatg tctgtttcat
     7081 tatttgctac tgcttgatag ttcatgactt cagctaagcc taaaacttcg ggtttttgta
     7141 aaaaaggggc taattttttt gcatttaaat ttgcaccatt ggtctcaaaa ggggttacag
     7201 gtacacaaga aggtagcata aaaaagatat tcatgagtgt ttgtcttgca tcttctatca
     7261 taaactcaag acctgtactc ccagcaatgt ttcctatttc atggggatct gtcactacag
     7321 tagtaactcc atgaagcaag gatacttttg aaaattcgct tggtgataaa agtgagcttt
     7381 cgatatgcac atggccatcg atgaatcctg gcactagata ttgcccatta gcattatgta
     7441 cgctttttcc ttcgtattca ccgattccta cgatcattcc ttcgcagatt gctacatctt
     7501 tttccatgat ttctttcgta aagacgttga cgatcttagc atgcttgatc actaggtcag
     7561 caggcttttt tttacttgct acttcgatat atttccttaa tgttgtttcc aatggagtgc
     7621 ctcccaagat aaatttttat aagtataaca taaattcggt ttttgccaat atttttttgt
     7681 aaattcattt tatttgaaac aaaaagatca aaaagacgta tgttttttgt ggaggggaga
     7741 ataatggaaa taaaacgatt atcggatcaa atgataagtg aaagttttgc caaacatgcc
     7801 caagacgtat atatattgcc agcttcagaa gtattttaca tctattttcg ctatggaaaa
     7861 aaacgaacat tctggaagaa agtctcttcg gcagatggac agcaactgat ctcaagattt
     7921 aagtatttgg gacagatgga tatcggagaa aaaagaaaag ctcagttagg tgcatttact
     7981 tattctttag aaaaacagga agttcgtctg cggttgtctt ctgtaggaga ttaccttcaa
     8041 cgagagagtt tagtgattcg gctattatac ggtacttttg aaacacccca tcgttgcttt
     8101 agttctcgag attttgcaac tattttacag aaaaccgtca gacgtggatt ataccttttc
     8161 agcggaccag tcggatcagg caagacttct ctaatgtatc ggttggcttt ggaagaaaaa
     8221 ttgcaagtga ttactatcga agatcctgta gagatagaag aaccgttttt tctgcagcta
     8281 cagatcaatc cgaaaatagc acagacttat gatacacttt tgagactcgc attacggcat
     8341 cgtccagact tgctaatcat tggcgagata agagatgaat caactgcaca tgctgcggta
     8401 agagcagcac taacagggca ccgggttttt gcaaccgtac atgcacgcaa tcttgaaggt
     8461 acattggtca gaatccatga actgacaaag aaaaaagaag agctatctga atgtttagct
     8521 ggtgtcattt atcaagaact tttggaagat gccaatcacc atccttctgt tttatggagt
     8581 tatcaattta tggaggaaga atcagtaaaa aaatcatggg aggaaagtta tgaagaagct
     8641 caggaaaata tttggcagtc agaactattc taaaggatta acaagaagcc agcaaaacca
     8701 gtttgcacaa accatgggag aactgctttt atctggtttt agtctgcaag aatcccttta
     8761 tttattactc acgatccgtt cgtttccacc tgttattttg gcaaatatcc aacatcagct
     8821 aaaggaaggg caagcgttta gtgaggtact ttcgcaaata ggatatggag cagagcaact
     8881 ggtacagatc gagttagctg aacgtcatgg gaatctgccg acaactttgt tggaaattgc
     8941 caaacagttt cggcttgttg aaggatttcg caatgatttg aaaaagctgc ttgcatatcc
     9001 gatgctattg ctcacctttt tgctaggtat cttgtttagt atgcggttgt tcgttttgcc
     9061 tcagcttatt ggttcaggaa tggtcatgca agagcattgg ggtattcagc tgatacaagt
     9121 ctttcattgg tatgttctac tattcatcct agcggttact ctattggctt tactagtaag
     9181 gtatcgtctg atgaagttgt ctcctgttga tcagagtgaa tggataagca gacaattatt
     9241 gattggaccg atatattcct tgtatcaatc ggcttatttg tcactagaga ttggaaagct
     9301 ttttcatgaa ggtttcgaat taaggcaaat catagtctgc ttaaaggaaa cgaaacaaga
     9361 aagtctgatt cagcatcttg cctctaagtt gattcaagga ttggaggcag gtatgtcatt
     9421 ggctgagcag ttccagaatt atcgttttct atcagaagaa ttcagtaaaa ttgttttaca
     9481 gggagaggca aagggaagtt taggtaaaga actgattttt tacagcgaat ggacaagaaa
     9541 acagcttttc aaacgtcttc atcactggat gcattttatt cagccaatca tttttttggg
     9601 cgtagcggct ttgattttat tagtttatgc cgctgtttta ttacctgttt acggaagcat
     9661 agaggaggtt ttgccatgaa aaagagaaaa atatcttatt atagcggttt tacattgatc
     9721 gaaatgctaa tcgtcttact gatcatcagt gtattagtgt tgttgttcgt ccctaatttg
     9781 tcgcgttata ggaatcatgt agaccaagaa agtcgtgaag cgatcatcca gttggtagac
     9841 acacaaaaag agctctatgc gcttcaaaat aatggaagag ttcctactgt ggaggaattg
     9901 ttgaatgaag gctatatcaa acgagaacat gcagaaatct atcagcggcc ttgaaggtaa
     9961 ggttttagca tcaggatata cgttgtttga atcactctta ctgattttat tacttacaac
    10021 gatcctttct tttccagttc tcgcattttc tgcatggaag aatgaattgg cagtctatca
    10081 atttttcggc caatttgaaa aacgaatcta ttctacgcaa aaaatcgcta ttgtgaatca
    10141 gatccaaaca ggcttttatt ggaatagtga agagaatcaa atcatttttc atgttccgaa
    10201 tccagataaa ataaattggg aagtattaga tattcctgat gagattacct tgaaacgcca
    10261 tgcttctatt acttttgctg ctagaaccgg gaatgaaagt tcactaaaag cttatcaatt
    10321 ttattgggaa gaaaaagagc ggacgattac gtatcaattc cagataggga gtggtcgcta
    10381 tacaaaaaga attgaataaa acaggatttg ttttgttgga gtcccttaca gcgcttatga
    10441 tcgcttctgt ctgtgtctac ttagtgagct ttggccagct gtttttgctt caggcagaaa
    10501 agaaaagtgg ggaggaactg ctatttattc gaatgattta tgaagatatc aagacgcagc
    10561 ggatgtatca caaaagacca gagcgaaggg aagaaatgat cccatacgat atccaaattg
    10621 tcgataataa tggagtaatc gaaaaagtgt ttgtcaatat ggagaaagaa gcgatcagta
    10681 ttgaaaaaac aagtaattcc agcatttacg ctgattgaat gtctaattgg actgatgata
    10741 ttgagcagtc tattgctcac attcaatctt ttgattcagc aaaccatcca agtaagtgaa
    10801 tttttgcagc gtaaagatca aaaagagtgg ctaatatttt tagcacaatt agaagaagaa
    10861 ctcaagagca gtcacaatgt ctctgtgaaa aatcagcaac tgcactacac gatcgaggat
    10921 aaacagtata tcatcgaaag atatcaggca atgatacgta aaagaagaac ttccggcgga
    10981 catcaaccta tgttaacaag tatcactgag cttcaattat tggaaaaaga acacacaatt
    11041 tctttttatg tacactttga aaatggagaa gatggatatg ccatatggac aaaaaacgat
    11101 cagtagaaaa tgttataagg gctctatttt attttctgtc ttgcttttat ttcttttatg
    11161 tacgttttta ttgatggcag cattagaaag ctaccgtttg tcaatggatc ttaatatacg
    11221 aatcaaaaat tattatatag caaaaataat gaagcagatg gctttatcgg aacagtctac
    11281 gcttgccgaa agttcggaag gagtttttta ctacacaact ggatctgtaa catatcagtg
    11341 ggtacaacag tccctattct tagaggtgga agtctcaccg tttatctttc ggttcacaga
    11401 agaagtaaaa aatgatacag atactagtac agaatgatgg aatgttcgtg aaaacgccct
    11461 cttttctgtt ataacactgt ttttaataaa aaaatgtttg ccgtttcaca aataaaaaac
    11521 agtgaaaacg attgaaattt tgtcaattgt tcgatagaat taaacaggtg aaaaaattgt
    11581 tataatatat ctagaaatga ggtgcaccat gttaccagaa aaaatggaac aagggtttaa
    11641 tcaaaacctg gaagctattc agcttttaca gaatgcattg ggaacttcct ttttagaagc
    11701 ctatgtcgaa aatgcagaaa atttaataga tgattatcaa gtacgtgtgg tggatggagt
    11761 gccgacaaaa gagacaacgc aacggatcac agctctttat gaagagttga agaaactttc
    11821 tttcgagcca gaagaatggc gcagactgtc tcaattactt ttattgaagg gcagccagac
    11881 agaacatttg caggcgaacc atcaactcac tcctgatagt atcggttttt tatttgtttt
    11941 tcttattgaa caactataca caaataaaaa agcaccggtt aagattctgg atattgcggc
    12001 aggaatgggc aacttgttat taacagttct tttaaaccta tcgaatgcgg ggtaccaaac
    12061 agaaggaatt ggtgtggata ttgatgatac attgttggct gtcgcagctt ctacaagtga
    12121 tttgacacaa gcaaatgttc aatactttca tcaagacggg ttgcaggaat tattaataga
    12181 tcctgtcgat tttgcaatca gcgatttgcc aatcggttat tatccgaatg acgaaaaagc
    12241 aaaagagttt ttaacaagta ccgaagaagg ccatagttat gcacatcatt tattgctaga
    12301 gcaatcaatg aaatatgtga agccggatgg ttttggtttg tttttgatgc cgagtggatt
    12361 tttagaaacg gatcaaagtg aagaaatcaa aaaatggttt aaagaagaag gttatcttca
    12421 aggaatgatt caattgccag atgaattgtt ccgtaataaa caatcgcaaa aaagtatttt
    12481 gatcttacag aaaaaaggac cacaagcaca acaagtaaaa gaagtcttgc ttgtgaaact
    12541 tgcttcatta aaagaaccag agaaggtgac ggaatttttc aacgagttta aaaactggaa
    12601 atcatctgct ctgtaatcat tcaacgagaa ggagagtcat atgtctaaaa caattgcaat
    12661 caatgctggg agttcaagtt tgaaatggca attatatcaa atgccagaag aaaccgtagt
    12721 tgcgaaaggg atcgtcgaac gtatcggttt acaagattct atttttacca tcaagtacgg
    12781 cgataatcaa aaattcgaac aaatcatgga tatcgaaaac cacgaagtag cagtaaaaat
    12841 gctgttagat cagttgattg aactgcaaat tttaagctct tatgatgaaa tcaccggagt
    12901 agggcaccgt gttgttcacg gaggagaaac ttatggggat tcagtagtga tcgatgagaa
    12961 agtaatggac cgcattgctg aattagcaga atttgctcct ttgcataatc ctgcaaactt
    13021 aatggggatc aaagcgttta aaaagatttt acctgacatt ttgagtgtgg ctgtattcga
    13081 tacatctttc cactcaacaa tgccaaagca aaattattta tatagcttac cggtgtctta
    13141 ttatgaagat ttcaaagtaa gaaaatatgg cttccatggc acaagtcatc gttatgtgtc
    13201 ggaacgtgcg gcaaaattat tgaacaaacc gatcgaagaa ctaaaaatca tcacttgcca
    13261 cttaggaaat ggtgtatcga tcacagcagt cgatggaggt aaatccatgg atacttctat
    13321 gggattcaca ccattagctg gggtgacaat gggtacacgt tcaggtgata tcgatccagc
    13381 tgttctccca tatttaatgg aaaaattaga gattgatatt gatgaaatga tcaatgtttt
    13441 gaacaagaaa tctggtctgc taggcctaac aggattgtct agtgatatgc gtgatcttga
    13501 aaagaactat gaaaaagaac atattcgttt agcttatgac attttcgtgg accgcatccg
    13561 aaaatacatc ggcggttatg tgacaacgat gaatggtgtt gatgcaattg tctttactgc
    13621 tggtatcggc gagaatgacg gacatgtacg tagtgaaatc atcaaaggta tgtcttggtt
    13681 tggttgtgaa atcgatccag cgttgaatga attgcgtggt gaagaagtag atatctctac
    13741 aaaagaatca aaagtaagag tattagtgat cccaacagat gaggaactaa tgattgcccg
    13801 tgatgtggag cgtctgcgcg gctaagccaa agcattcgtt aggataaaag atgaaacgca
    13861 tgcaagaggg aattcttgca tgtgtttttt ttgcggagaa gaccgcaaaa tggtaattta
    13921 acttcaattt cccttctttt ttacaggatc ttcttaggga attttgcttg gaaaagtggt
    13981 caaaagggaa aagactggca ttttatttat tgattttcag gaaaatccca atattttatt
    14041 ttcattcatc tttataaggg acattacata tgtaaaaaga aaaaacattt ttagatcagc
    14101 ctcgtgggct gtcgacttta ttcttcgctt tatgtttagc tagaaaaccg atccgaaaca
    14161 ttcattgatc gaataataaa aacttgattt tccacctgta ctttgcctga atcgtgaaag
    14221 cacaggtatg aaaatcaagt tttttgttgc gaaaaaacgt cctagtcaat cagttggccg
    14281 agcggctggc ggctgatctg ctcctgatca ataacgagtg cttcttggaa gtgttgctgc
    14341 aaaaagtagc ggtcatggct gactgctagg agcgttcctt gatagtcttg cagcatttct
    14401 tcaatttctt ctcgagcata gctgtcgagg tgattggttg gttcatccaa aatcaggacg
    14461 ttgatttttt ctaaaaagag tttcagcaac gccaaacgga ttttttcacc accggataaa
    14521 tctttgatcc gtttacccac atcgctttga taaaagccac ttttggctaa ttgccgtcga
    14581 gcgacttctt cgtttccagt caatgtgatg gcgtaagcca gcaaacggct atcgggatcg
    14641 gcaaactgca gctgctgcgg caaataaccg atttgcaagc tactacctaa tttgatggtg
    14701 ccaccgtaag gcttttcctc accgagtaat aatttgagta aggttgattt tcctgcacca
    14761 ttgtcgccga tgatggctaa atgatctcca cgatagactg caaaactgct gtctttgaat
    14821 agtacttgat cagcgtactg ctggtgcaag ttttgaatga tgaaaacttc tttgccagat
    14881 tttccaccat tagacaagct ttgcagccga tttttcgggg gaggtggcgg tttgacgagt
    14941 gtcagtttcg ccaggcggcg ttccagttct ttggcttttt tgaagaagga ctcattgtct
    15001 ccttcatgtg cccactgacg aaaacgacga atcatgactt tcaaccgctg gacttctttt
    15061 tgctgcagtt cgtaattttt tgtcagctca gccaagcgag cttctttcaa gactcgaaaa
    15121 cggctgtagt ttccgggata ctcaatcaat gcaccttctt ccagttccca tgtttttgtg
    15181 gtgacttgat cgagaaattg ccgatcatga gaaacgacca gaaccgattt ttttgtattc
    15241 tgtaaatagt tttccaacca gcgaatactg ttaagatcta aatgattggt cggttcatcg
    15301 agtaaaagta aatcggcatc ggctgctaaa attttggcta aagcgacttt aactcgttcg
    15361 cctccgctaa gttctgttaa ccgtgcttcc tgacggtgtg gaatccctaa gccttgcaag
    15421 atcgctgcaa tccggtcttc taatacgtaa ccgcctagtt cttcaaagcg atgctgcagc
    15481 tcaccgtaac gggccagaca cttctctaag tttgtatctg gttctgtcat caatgcttcg
    15541 tagcgactca tttgctgttt gaggtcctgt atctcagcgg cagaatggag caagtagtgg
    15601 ttgacggtcg cttcggaaac tgtaagttct tgttcgaccg tcccgatctt taagcctttt
    15661 ttgcgactga taacaccgct gtcgacacct tctctgccgg tcaagatttg taaaaaggtt
    15721 gatttgccag ttccgttgat accgactaag acaatttttt caccagcttc aatttgacag
    15781 gataaatcct caaataatgg attcccgccg attgtttttt taatagaaat tgcttgtatg
    15841 atcatgatct ctctccttga gaccactcaa aaaggccatg gttatccatg gcctgtgtta
    15901 tttaaactca gattatcatg agaaaaatac cttgagatgg ccattgattt tacgtgaatt
    15961 caactgtttt ggacaaactt gtcccgtcaa cagcaactgt aaaaccaatt gcatggctaa
    16021 atgcgatatg gtatcgttgg taattttcat tacttgttat tttagtcatg cgcatcatag
    16081 tattttccct ccttcgtgta gttgtctttt ccagtgtacc tgaaagtgtc agaaagttca
    16141 actagcgctg acatttactt aatatttatc gatgtgtaat tctgtcagag agcttttttt
    16201 atctgactga gggtaatgtc tcgtggacca catcattgta ttctgagcta aaccagcgtt
    16261 caggcgaaat catatggtgt ttgataaaaa actttgtcta catgacttcc ttttgccatt
    16321 tgcttaatac cgttgcatta aacagctctt ctctttggaa atctcaccaa catcgcgttc
    16381 aggagtatcg ttgtggttgc aatggcaagc ggtagccttt catcaattct tgaatgattt
    16441 accgaatgta gcacttgcct tctcgcggta cccgaacccg aaataaaatt ttagtttggc
    16501 gataaaatcg gtatccgcca tcgatacttc atattttttc gtatcagatt catcggaaac
    16561 ttccacattg acgaaccaat aagctttggc atgcttaggt cgttttaaac cacaccaggg
    16621 cgactaatcg ccctggtgtt cacttttttt tattttactt gaactcattg actgttttcc
    16681 ctttatttta aacattataa agaccgatga gtgcttgccg cgtcttttgt ttttatcacg
    16741 gttttttgtg caaaaaaacc tgccgatagt tggcaggtcg tctacgtgtt aagctttggt
    16801 gtggtcttta gctggcaatt ggttttctaa atcagttcgg acaacaagca catcgcaagc
    16861 tgcgtttcta ataacatatt cagaaacaga accaataaat aaacgttcta cagcattcaa
    16921 tcctgtggct cctaacataa tcaaatctac ttgattatct tctgggattt ctctggcaat
    16981 aatttgtttt ggtgagccat attctacgac agaggttaca ttattcaagc cagctttctt
    17041 cgcattgctt tcataatctg ccaatgtttg ctttgccatt tcagtagctt gttctgcaag
    17101 cattccatca aaggatgaaa cggtttggaa agaacgtgtg tcaatgacat gtgctaatag
    17161 aagttctccg ttatttcgga tagctacatt gaccgccttt ttaaaagcta attcagcttc
    17221 atcagaacca tctacggcaa ccatgatttt tttgtattgt tgtaacataa gcattcactc
    17281 ctcaagtcaa tcgtattgta ctcttttatt gtagaacact ttagggaaaa agagaattat
    17341 ttactgcttt ggttcagcaa ttttttttgc aaatcggata ctgaaggtgg cagaaacaat
    17401 aattacgatc agtaaaaaga ccaaggcaat aaagcgttga ttgaaaaaag ctgttaaaat
    17461 acccattacg cctaaaacag catatgcagc tccgtaaaat tgtaggaatc cttgattttt
    17521 atcatttttg ttgatcaata caaaaaagat atcctgttta ttaaataaat aatagctaac
    17581 gaataataat actaaagtgg cgataatcat taaaatccta atcatgaaca aatcctccaa
    17641 aataagcaac aaatagaaaa agcaatttgc caagagcaaa ttgctttatt ctttaaaaga
    17701 tggtaatctc cgttaatgcc gttgtttgtc ctgtagtatt gatcccgcga attcaagcag
    17761 gtgggtccca tggtcgtagc ttcgaactac caaatgataa ggcatgttac cagcagtgac
    17821 acaagacggg tcccaagata tcaataaaaa tgttcggtca acacacaaag aggacaattc
    17881 gaacatcaca gatttcccac ctctatagta gcatatgaat ttaaaaagag caatgagaga
    17941 gctcgcagaa acagaaccgt tttcttgtct agttttttgg aggatgcttt ttccactctt
    18001 ttatacggta atactgttga cccagagcct gttcatattt ccctgtatct tttggctgat
    18061 aatactgagc attcttcagt ttatctggta aatattgctg attgacccaa gcctgatcaa
    18121 aatgatgagg atactgataa cccacgccac ggttgagtga tttcgcacct ttgtaatggc
    18181 tatctctcaa atgatcagga acatctccag ctttgccctc tcgaatatca gcaagtgctg
    18241 catctaatgc catgtaggca gaattggatt ttggcgacaa gcaaagatcg acgacgacgt
    18301 ctgctagtgg gatacgtgcc tcaggcaatc ccaatttttc agcagctaat acagcgttca
    18361 ctgtacgagc cgcagcagca ggattaccta aacctatatc ttcataaccg ataaccatca
    18421 gacgccggca aatacttgcc agatcacctg cctcaactaa acgggcgaga taatgaagtg
    18481 cagcatccac atcacttcca cgaatcgatt tttgaaaagc cgaaataacg tcataatgag
    18541 catctccatt tttgtcatgg gtaagagctt ttctttggac acattcttca atgatcgaca
    18601 gtgtaagatg aatccttcct tctttatctg aaggagtcga tagtgtagcc agttccagcc
    18661 cattcaatgc actccgcaaa tcgccgttcg ttgcacgcga aaggtgaaga agagcatctt
    18721 cgtctaactg gattgcttgt tgtccaaggc cgcgttcttt atcccttaaa gcatgttcga
    18781 ctgccaactg gatgtcttgt tctgttaatg gtttgacttc aaagatctgt gtacggctgc
    18841 ggatcgctgg attgatggta atataaggat tctcagtagt cgctccgatc aagatgattc
    18901 ggccactttc taaatgagga agcagaaaat cctgttttgt tttatctaat cggtgtactt
    18961 catccaataa gaggatcact gtaccactca tttttgcttc ttccgctaca acttgtagat
    19021 ccttttttga atctgtagcg gcgtttagca tgcgaaatgc ataattagta gagccggcga
    19081 tcgcacttgc gatactcgtt tttccagtac caggaggtcc atatagaatc atggaagaga
    19141 gcatgcgagc atcgaccatg cggcgtatga tttttccttc cccgactaga tgctgctgac
    19201 caacgacttc atcgatcgtt cgaggtctca tgcgataagc caatggctgt tgcataggat
    19261 tttcctcctt cttgttttta cgtcccttaa ttatatcata tttagcaaga tgcctagaaa
    19321 gaacagcctt ctgttgtttt gttttcatag cttatccaac taatattgtg tatgataaag
    19381 taggtgagaa aaatgacgga taaacaaaag atagatgaat ttttttggaa taaaacgacg
    19441 ccagaagtgg cacgggattt attggggatg tatttggagc atgacacacc agaaggaaga
    19501 ttagctggat atattgtaga cgcggaagca tacctaggtc cagaagacga ggccgcccat
    19561 agctatggtt taagaagaac accgcgtgtg cgggcaatgt atgaaaaacc aggtacgatc
    19621 tacctttaca cgatgcatac tcatcggatt ttgaacatca ttactcagcc agaagggatt
    19681 cctcaaggag taatgatacg tgcaatcgaa cccgccgcaa tgattgatca aatgagtaaa
    19741 aatcgtggcg gaaaaactgg tccagatatc agcaacggtc cgggaaaatt agtggaagca
    19801 ttagcaattc ctcaagaact ttatggtcag tctatagcag atagttcgtt acgcttagtt
    19861 tttgagaaaa aaaagacacc aaaaaagatc aatgctttgc ctagaatcgg tatcccaaac
    19921 aaaggtgtat ggacagaaaa gccgttgcgt tttgttgtgt cgggaaatcc ttatatctct
    19981 ttacaaagaa aaaaccaaat cgagaaagat tggggttgga ggaaagaaaa tgaaaaagaa
    20041 ggatctatta acatatttag atgaaataat tgaaaaacaa gtgacagact atgatgtagc
    20101 tttagattgg gatacaaaaa atcatacgat cgagatcgtt gttcgcttgt ttgcagaaaa
    20161 taaagcacaa tttgagattg atgatgcaga agggatcgtt tcgcaagaac caattatcga
    20221 atttgaagac ggcgtacttc tttttaatcc tcagaaatct gtttttgatg aacgcaatta
    20281 tctagcagta atcccttatg aaggaaaaaa agggttggca aaatcagtag ctgattcttt
    20341 agtggaatat ctaaatgaag tattagctca agggcaaagc gatttgcttg attttttgga
    20401 tgaagacgat gacgaagctg tattcgagct tcactgggat aatcagcaac tagcagaatt
    20461 agtagaagta aaacagcaaa atgaagcaga tacttattta ccgtacccaa gttattaagg
    20521 agtgacagat atgaaatgga atgaagtaaa agtagaaact gaaagtgaag cagtcgaagc
    20581 agttgctaat attttgatgg aggcaggggc gagtggtgtc gcgattgaag attctttaga
    20641 tgttgaacat tttcagtcgg atcaatatgg tgagatttta gataaaaatg attttacatc
    20701 gctgaaagaa ggcgcactag tcatggctta ttttccggaa acgactttct taccggaaat
    20761 cttgcctttt atcaaagatc gtatcacaca attacctgag tttggtctag ctattggaaa
    20821 aaataaagta acagttagtg aagtagagga aagcaactgg gcaacagcct ggaagaaata
    20881 ctatcatccg gtgcgtatta cgcgaatgct gacggttgcg ccaagctggg aaaactacca
    20941 gacagaagat cctctagaaa aaattatcac actagatcct ggtatggcat ttggcacagg
    21001 cactcatccc acgacttcct tgacattgac agcactagaa acagtcttgc gtggtggtga
    21061 aacattgcta gatgtgggaa ctgggtcggg cgtgctaagt attgcagcaa aatatcttgg
    21121 tgcaaaagat gtctatgcct ttgatttaga cgaagtagcc gttcgttcgg cgaaagaaaa
    21181 tatggatatg aatgaaatag ccaaagatgt ccatgtatca gctaacgatc ttttgacagg
    21241 aatcgagatt gaaagtgatg taatcgttgc gaacatttta gcagatatca tcttattgat
    21301 gatcccagat gcttggcgtt tactgaagca aacaggcacg ctgatcgttt caggaatcat
    21361 tgaagagaag aagcaaatgg ttctggatgc catgttagaa caagggttta tcgtggatca
    21421 aattttccag caaaaagact ggtatgcaat tattttgaaa aaaccagagg tggaatagat
    21481 gcagcggtat tttttaaaag aatcatatga atcaaaagac tattttgttt tagaagggga
    21541 aaactatcac cacattgtac gggttatgag gatggaaccc gaggatgaag tttttttggc
    21601 attcaatgat caaatagcca ttgttgcaga aatcacggag attactgaag aggctgttta
    21661 tctgaaagaa attaaaaaag aagaaaatca aaaggaactg ccaagttcga ttaccatagc
    21721 gagtggctat cctaaagggg aaaagctgga ttggatcgtt cagaaatcca ctgaattagg
    21781 agcgcaagct tttcttggtt tcccggcatc agcctctgtc gttaaatggg atgcaaaaaa
    21841 acgtatgaaa cgacaacaac ggcttgaaaa gattgctcaa gaggcagcgg aacaatccca
    21901 tcggcagatc gtgccatcag tagagttatt ggaaacgaaa caagatttat ttgagcggct
    21961 agatgattat gacgtggtct tggttgctta tgaggaatca gcaaagcaag gcgaaaaatc
    22021 aaatgcagcc aaaattctta gtgaaacaaa accagatagt cgaatcttag ctgtctttgg
    22081 accagaaggt ggattatccc gcgatgaagt cgattatttt gtagaacaca aagggatact
    22141 ttgtggatta ggaccaagaa tccttcgtac agaaacagca cctctttatt tattaagtgc
    22201 tgctagctat caatgggaat taatgaatcc ttctttcagc taacgttctt cattaataaa
    22261 agagtatcga cagaaagaat tgaaaaaata gtcaacaatt tgtataatag aaacaattat
    22321 ttatacaatg gatacgaaaa atcgtttgaa aaagctttta aaaagcgaac atttccgtaa
    22381 aaaaacgtat aactgcacgg attttgggac aattatcaaa cagatatttg tcccgattcc
    22441 cgttttaccg aaaaaagaaa gggggatcga ttcctgtgac aaaagaagaa attttaacgg
    22501 gacccggtgt cataaagctc gttagtcaat acatgggtcc cgaacatgta gcatttgttg
    22561 agaaagcatg cgaatacgcg actgctgcac atgacggaca gtttagaaaa tcaggcgaac
    22621 cctacattat tcatcctatc caagtcgcag gtatattagc agatttaaaa atggatcccc
    22681 atacagtggc tacaggcttc ttacatgatg ttgttgaaga tacagaaatc actttagaag
    22741 atctgagaga agaatttggc gatgacgttg ctatgttagt ggacggcgta accaaattag
    22801 ggaaaataaa atataaatcc cacgaagagc agctagcaga aaaccaccga aagatgctgc
    22861 tagcaatggc tcaagattta cgagttatca tggtcaaatt agctgataga ctacataaca
    22921 tgcgtacgtt gaagcacctg cgagaagata agcaaagaag gatcgctcag gaaactttag
    22981 aaatctatgc gcctcttgca catcgtctag ggatcagccg gatcaaatgg gaattagaag
    23041 atacagcact tcgttatcta aatccaaaac agtattaccg tatcgtccac ttgatgcaga
    23101 cgaagagaga agaacgtgaa aaatacgtaa gtggtactgt tgaagatatt cgaatagcga
    23161 cagaagagct ggggattttt gcagaaatct atggacggcc aaaacacatt tattcaatct
    23221 atcgtaaaat gaaagatcag aaaaagcagt tcaacgaaat ttatgacttg ctagctattc
    23281 gagtgatcgt agattcaatc aaggattgtt atgctgtatt aggagcaatc cacacaaaat
    23341 ggaaaccaat gcccggcaga tttaaggatt atattgccat gccaaaagct aatatgtacc
    23401 aatctttgca tactaccgtg atcggtccgg caggaaatcc ggtagaaatt caaatccgaa
    23461 cacaggaaat gcatgaaatc gctgaattcg gggttgctgc acactgggcc tataaagaag
    23521 gaaaaaatga aaaagtagaa ccagatggta tgacgaaaca attaagctgg ttccatgaga
    23581 tactcgaact tcaagacgaa agctatgatg cttctgaatt tatggaaggc gtaaaaggag
    23641 atatctttag tgataaagtc tacgtcttca caccaaaagg agacgttact gagttaccaa
    23701 aaggatccgg accattggac tttgcataca gtatccatac agatatcggt aacaaaacca
    23761 ctggtgcaaa agtaaatggc aaaatggtgc agcttgatta caaattgaaa aacggagata
    23821 tcattgagat catgacttct ccaaattcat ttggcccaag tcgcgactgg ttgaaattag
    23881 ttgctactag caaagcaaga aataagatca aacgtttctt caaagcccaa gatcgagaag
    23941 aaaatgtgat caaaggccac gaatccgtgg tcaaatgtat tacagatcta ggatttacgc
    24001 ctaaagatat tttgacgaag aacaaactgc aagaagcact cgatcgtttt aattatcaaa
    24061 cagaagatga tctctatgca gctgtagggt atggagaagt tagccccttg acgatggcca
    24121 atcgtctgac tgaaaaagaa cgtaaagaac aaaaaatcga gcagcaaaag caagaagcag
    24181 aagaaatcat gaatcagccg aaaaaagaac ctgacaaaat gaaagtacgt catgaaggtg
    24241 gcgttgtcat tcaaggggta gaaaacttac taattcgtat cagtcgctgc tgtaatccga
    24301 ttcctggtga tgatatcgtt ggttatatca ctaaaggcag agggatatcc attcatcgtc
    24361 gagattgtcc gaatgttcag cctgacaaac caaatgtagc agaacgtttg attgaagtcg
    24421 aatgggaaga tacatcgaat acacgaaaag agtatgatgc agatttggaa atttacggct
    24481 ataatcgttc aggcttattg aatgatgtac ttcaaacagt caatgcgcta acgaaaaatc
    24541 tcaacagcgt tgaagcacgg acgaataaag ataaaatggc gacgatccat ttgacggttg
    24601 gtatccagaa tttatcccac ctaaagagta tcgtggataa aatcaaagca gtacctgatg
    24661 tctacagtgt acgccggacg aatggatagg aggacagata tgagagcagt tgttcaacgg
    24721 gtaagtaaag ctgctgtgtc agtcgatcag caagaaattg gaaaaattgg ctacggccta
    24781 cttgttttat taggcattca tgaaaccgat acgcaaaaag atgtggacta tctggtaaaa
    24841 aaaattgcac aaatgcgtat ttttgaagat gaacaaggaa aaatgaatat tagtgtagaa
    24901 gatgtgggag gacaaatttt aagtatctct caattcacac tgtttgcgga tacgaaaaaa
    24961 ggaaatcgac caagctttgt ggaagccgca cgaccagaaa cagctattcc tttatacgaa
    25021 tcattcaatg aaggaatcag aagtcgcggc atcacagtcg ctaccggaca attcggagca
    25081 gatatgtcgg tttctctcgt caatgatgga cctgtaacga tcattattga ttctaaaaac
    25141 aaataaagta atctggtttt cactagtaaa aactatgaaa cacgtcgctt aagttagact
    25201 gataaactct aacttaagtg gcttttttgt ctgcaaaaag cgcttctttt gtcagcagtc
    25261 ggaaaaaaag ttaagcttaa attatacaag ttatttttta taggaggttg ctgaagatga
    25321 aagaagtacc agtagtagat atcaaaatca cagatttaaa aaaattgtat caaaaaggat
    25381 acaacatgtt ggaggagtta cgccatgaag cggacgctcc cgtcgtcaaa gcaaagattt
    25441 tcaataaaga agctatcacg atctacggat catcagcagc aaaagtattt tatgatccaa
    25501 ggaatttcaa acgaaaaggt gctatgccaa aattagtatt gaagacactt tttggtcaag
    25561 gtggagtaca gactttggat ggagctgctc atcaccatcg gaaaaatatt tttatggatc
    25621 taatgacacc ggaaagaatg gaagattatc atcgtatctt agataagaat ctgacacaag
    25681 cacttgaagc acagcatggt caatttgaac tatttgattt aagtaagatg gttttcttta
    25741 catctatttg cgagtgggca ggtatcaatt tatcagcgat ttctaaagat gaggtcgaaa
    25801 aattagcaga atatcaaatc tctatgatca gcggaacgtt tacttctccg attgatcaca
    25861 taaaaggtgt cgaaaatcgc aaaaaatcag aaaaatgggc gcaaggcttg attgaagaag
    25921 ctcgacaaaa tcctgtggct ggaaaagaaa atgtggcatt atatgctttt gccaatgcca
    25981 cagatttaga tggtcagctt ttacctctgg aagtggcagc tgttgaatta ttgaacatca
    26041 tccgtccaac ggttgctcta actgtgtggg cggcattgat gggacatgca cttttcagta
    26101 gaccagattt gtatcagcaa ttaaaaaatg acttttcgac gttacaagat ccgtttattc
    26161 aagaaatgag acgctattat cctttcttcc caatgctacc tgcgatttcg ctgaaagaag
    26221 tagaagtaga tggttatcgt ataccagaag gcagttgggt tattttagat ttatatggaa
    26281 cagaccatga tgaacgaaca gttgaagcgc cagattcctt tatgatcaaa cggtatgtag
    26341 ggaaagcaaa agatatttcc tataaagaag aatatgagat gattgcacaa ggcgggggaa
    26401 attttcgaca aatgcatcgc tgtgcaggag aatggatcac tcttcatagt ctacgtgtgt
    26461 tttccgatca gctagtaaat aagtttgagt tctctgtacc tgaacaagat tggacgattc
    26521 cattcaatca gtttccgact tatccgaata gccgagcgct actttacaag aattagatta
    26581 gcataaactt atcgaaaaaa aggaagatca aacagtgctg atcttccttt tttcatatta
    26641 ctataaattg ataaggtcaa ttgatcaaaa tccacgaaat gcttcatcaa tggtttgata
    26701 atcttcattg gataaatgca aatctaaagc ttggacattg tcagatactt gctctggacg
    26761 cttagctcca ggtataacta cagacactcg tggattcttc atgtaccaag ctaaaacaag
    26821 ttgcgcagga gtggcttggt atcttttagc aatggggcga agtacatcga cagcggttaa
    26881 aatttctctg aaacgttctc cttggaaatc tggatttctt tttcgaggat ctccttcacc
    26941 aaattgtttt tcctcaccaa gttcatattt tcctgtcagc aaaccggaag caagaggaaa
    27001 atagggaaca aaactgattt tgtttttttc aagataaggg aataattctt tttcagcctg
    27061 ccgatggatc aaactgtatt tatcttctac tacatcgaca tatccatctg cattcgcttc
    27121 tttcaattgt tctaacgtga agtttgacac accgactgct cggatttttc ctgcttcttt
    27181 taattcgtgc aatgtagcaa ctgattcatt ttttggtgtg ctttcatccg gaaagtggat
    27241 ataaaaaatg tcgatgtaat cagtttgcag acgttttagt gcatcttcca ccgattgttt
    27301 gagaaactca ggagaattat caaatgttcg ccctttattt ggtacatgag cagcttttgt
    27361 agcgatgacg acacgtgagc gatcatattc tttttccttc agtacctcgc caatcagttc
    27421 ttcggaacgg ccgtttccgt aagcataagc ggtatcgatc aaattgatcc cgctattcaa
    27481 agctgtacga actacttgtt ttcctgtctc atcttccaaa ccgtcaaaaa gattatgtcc
    27541 tccaacggca tttgctccta atccaagctg ttctgcgtag acttgtgaat gtccgattct
    27601 tatttctgcc atagctgatt cctccttttt gtctagtcta aaacaaaata ccaaaaagct
    27661 gaactaaaac gtttttgtga ggggaaatag tcagatatct cttgactttg agactggttt
    27721 ttagtaggtt ataagtaata tcgaatgtca atgaaagagc gagtaagttt ttgaaacttt
    27781 acaagagagg atcatcgagg ctgaaaatga tctaaagttt cattagccta aagacactct
    27841 ggagatggac gagtgaaaag ccgtcccgcg tacgagcgtt attcgtaaga ggaaagagaa
    27901 atctttcaaa attaggtggt accgtgtatg aatttcattc acccttgttc atgatgaaca
    27961 agggtgattt ttttatttac atagctaaga aagggagaga aaactatgag tttccaaaga
    28021 ccaaaaggaa caaatgatat tttgccaggc gaatcagaaa aatggcagtt tgtcgaagaa
    28081 acagcacgtt tattattcag agattatcag tataatgaaa tgcgtacacc gatttttgaa
    28141 cactatgaag tgatatcccg aagtgtaggg gatacaacgg atatcgtttc aaaagagatg
    28201 tatgattttt atgacaaagg ggaacgccat gtgacgcttc gaccagaagg tacagcacca
    28261 atcgttcgtt cttttgttga gcataaattg ttcggcccag agtatgcgaa accgttcaaa
    28321 actttttata tgggaccaat gtttcgctac gaacgccctc aaaaaggacg gttacgtcaa
    28381 ttccatcaaa tcggagtaga agcatttgga tcagaaaacc ctgcaactga tgtggagagc
    28441 atggtgatgg cacttgattt cttcaaacag ttggggatca cgcaaatccg gctagtcatc
    28501 aattcattag gtgataaaga aacgcgtcaa gcctatcgtc aagcattgat cgactatctt
    28561 ttaccttttg aatcagagct gagtgaagac tctaaacgcc gccttcatga aaatccatta
    28621 cgcgtattgg acagtaaaga caaacgagat caaaaatttg tggcagaagc tccatctatc
    28681 ttagattatc taagcgagcc tgctcaaaaa cattttgata ccgttgtttc catgctggat
    28741 gcattggctg ttccttatga aatcgacagt aatatggttc gcggactaga ttattacaca
    28801 catacgattt ttgaaatcat gagtgacgca ccaaaaatgg gggcacaagc aacgatttgc
    28861 gcaggcggac gttatgacaa tctagtggaa gaactaggtg gtccttcaac acctggcttc
    28921 ggatttgcaa tgggtatcga gcgattactc ttgactatgg aggcagagga agtagttatc
    28981 cctgcattta atgagttgga tgcttacgtg gtcgcactcg gagacgaaac aaatatcgaa
    29041 gcattgaaag tggtccaagc gattcgaaac tttggttttt cagctgaccg cgacttcatg
    29101 aaccgtaaag caaaagcgca atttaagaca gcagataaag cgaatgccaa attagtattg
    29161 gtgatcggtg aagatgagct tgctagtcaa acgattaatg tcaaagcaat ggcaagccga
    29221 aaagaaaaaa gttttccatt agaagagata tatgaacatt ttgatgaagt ctatgatgaa
    29281 atgactttat tcgagtagga ggaaaaaaat aaatggcaca aagaacgatc tattgcggat
    29341 tagtttccga acaacaatta gaacaaacag ttacattaaa aggatgggta caaaaaagac
    29401 gagaccttgg gggagtaatc tttatcgacc tacgtgatcg tgaaggaatc gttcaaatcg
    29461 tctttaatcc tgaaatcaac aaagaagcat gggaaatcgc ggataaatgt cgcagtgaat
    29521 acgtgatcga agtgactggg gaagttagaa gacgcggaga aaatgcagta aatcctaaaa
    29581 tggccactgg tgaattcgaa gtgattgcaa gtgaaatcgt gattttgaat caagcaaaaa
    29641 ccccgccatt tcaaattgaa gatgatcaag caatcagtga cgaaatacgt ttgaaatatc
    29701 gttatttgga tctgcgtcgt ccaaaaatga ctaaaaacat caaattaaga aatgcgacaa
    29761 caaaagcgat tcgtcattat ttagatgata atgactttct cgatatcgaa acaccttacc
    29821 tagggaaatc tacaccagaa ggcgcaagag actacctagt tccttcacgt gtccatgctg
    29881 gacatttcta tgcgttgccg caatctcctc aattgtttaa acaattgctg atgaatgctg
    29941 gattcgaccg ttattatcaa atcgttcgtt gtttccgcga tgaagattta cgtggagacc
    30001 gtcagcctga atttacgcaa gtagatattg aaacaacgtt cttaacagca gaagaaatcc
    30061 aaacttacac agagggattg attgcgaagg taatgaaaga agttcgcggt atcgaagtca
    30121 cattgccatt tcctcgtatg acctatgatg aagcaatggc tcgctacggc agcgacaaac
    30181 cggatacacg ttttgatatg gaattgatcg atctaagcga tacagtcaaa gaagtagaat
    30241 tcaaagtctt ccaaatggct cttgaaaacg gcggtgtcgt gaaagcattg aatgctaaag
    30301 gcgctgcaga tcgctactca cgaaaagaca tggatcagtt aggtcaatat gtaggacaat
    30361 tcggagctaa ggggcttgca tggctgaaag tagaagaaga cggattgaaa ggtccaattg
    30421 ctaaattcat gggagaagca acagaagcaa tcattaaagc aacagatgca aaaccaggtg
    30481 acttgctgat gtttggcgca gataaaagtg aaatcgtcgc tgctgcatta ggtgctatcc
    30541 ggacacgttt aggaaaagaa ttaggactaa ttgatgaatc gaaattcaat ttcttatggg
    30601 taactgattg gccacaattt gaattttcag aagaagaagg tcgatatgtc tctgctcacc
    30661 atccattcac gatgccaaaa gaagaagata tcccgttatt ggctgaagac cctgcaaaag
    30721 tctatgcaga agcttatgat atcgtcttga acggttatga actaggcgga ggttctatcc
    30781 gtattcatac tcgtgagctt caagaaaaag tactagaaac tctaggcttt acaaaagaac
    30841 aaatggaaga acaatttggt ttcttgttaa cagctttaga ttacggattc cctccacatg
    30901 gcggtattgc attaggattg gaccgttttg caatgttatt agccggagaa gataatattc
    30961 gtgaagtcat tgcctttcct aaaaacggaa aagcagcaga tgtgatgagt gatgcaccaa
    31021 gtatggtttc gcctttacaa ttgttcgaat tgaatattga tgtaacgaat atagaagaat
    31081 agccgataaa aaggtaaaaa ggatttgtcg aatacgcttc ttttgacaac cttgatttaa
    31141 ttgaaaaaca ctttataatg aagccataac atataaagcg gggcttttcc atgaaaaaaa
    31201 cattcgaaaa ttgggcttat catgattaca cgaccaagct ttcggtatct atcgtttatg
    31261 cgttgatcgc atcagtcgca ttgaatttct tttatcagcc aggacatatt tattcaagtg
    31321 gtattactgg tctagctcag attttgacga ctttgtcaaa atcactgata ggattcgaag
    31381 taccggtatc aacaactttg tatttactga atataccttt atttgtattg gcgtggttaa
    31441 aaatcgggaa gaagtttaca attttcactt ttatcacagt cactttgacc tccctttgta
    31501 tccatatttt accggaaaca gtcctatcta ccgatccaat catctgtgcg ttgtttgggg
    31561 gggctgtgat gggactaggt attgggctag cgttgagaaa cggcctatca tcgggcggtc
    31621 tagatatcgt gagtatcacg attcgtaaaa aaacaggaaa gtcgattggt tctatttcga
    31681 tctacttcaa tgcattgatc gtttttgttg ccggaattct atttggctgg caatatatgt
    31741 tttacagtac gttgtctatt ttcgtcagtg gaaaagtaac tgatgtcgtt tatactaaac
    31801 aaaagaagat gcaggtaatg atcgtaacga aacatccaga aaaagtagta aatgaaatcc
    31861 aaaactgtat gcgtagaggg atcacgatca tcaatgaagc agaaggtgct tataagcatg
    31921 ataagcagac agtattgatg acgatcgtta cacgatacga actgcctcta cttagagaag
    31981 caatgaaagc ttctgatcca caagcttttg taagtattgc agaaaacgtc cagatacttg
    32041 gtcgtttcta tgaggatggc atttagatcg agtttgacaa aaattcatga aatactgctg
    32101 tagacagacg ttcgtttgtc tgcagttttt ttctaccata gtagaatcag caaagaaaaa
    32161 ctgctttttt atttgaaatt ttcttaaagt tgaaaaaaag catgtttatt tttttaaatt
    32221 tatgggatga tagaaaaaaa tagtagagaa aggacacgat tctacttgct attttcactt
    32281 aatacccgta tattacgaat gaaaaaagtt tatgaaaagt tggaatattg aatggaaaaa
    32341 caaaaaaggt taaaaaacag ccgctacatc actgggtttg atgggctccg ttcattagct
    32401 gtggtgggtg ttatcttcta tcaccttctg ccaactagta tgaaaggcgg ctatttgggc
    32461 gttccgattt ttttcgtcgt atctggttat ttgatcacgg atctgctgcg gcaggaatgg
    32521 gaacaaaatg gaaaaatcaa tatttggcaa ttttatgtca gaagaatgaa gagactctat
    32581 ccggcacttg tttttatgct tgtgacagct tcggcttata tcactttgtt tcagcgaggg
    32641 ctgttaaaca atcttcgagg agttgtggtc agcagcttac tgtatgtcaa caactggtgg
    32701 caaatcaata acggtttatc ttatttcgat cgatttgcta atgagtcacc gtttacgcat
    32761 atctggtctt tagcagtaga aggtcaaaac tatttggttt ggccgatctt gtttgtattg
    32821 ttgatggtat ttgttcgtaa aagaaaatgg atcttttata ctgtgttagg tacatccttc
    32881 ctctccgcca tcttgatgat gatcttgtat acaccaggag gcgatccaac ccgtgtttat
    32941 tacggtaccg acactcgtct gttttctata tggttgggaa gtgcgcttgc gtttgtttgg
    33001 ccaagtacgc gtttaaagaa aaacatcccg aaacaagcga aaaatattct gaatatcgct
    33061 ggaggaatct cgcttttggc actgattctt tgcttcttct ttttagatga tcattacaat
    33121 ttcatctatt acggaggaat gctcctagtg agtctgttat gcgtagtgct agttgcagtg
    33181 acagcacacc ccggagcaag tttgaatcga tggctgacaa atccagtatt tacttggatt
    33241 ggcaaacgaa gctatggcat ttatctgtat cagtttccag ttatgatttt ttatgaagca
    33301 aaggtgactg atattgccga tcatgttttg ttgcatactt tagtggaaat cacattgatt
    33361 ctaatcatca gtgaactttc ttatcgatat gtggagcgtc cgctagcacg ttttgattat
    33421 ggtcgtacat tccaagtgat gaaaggatgg ttcagcaaac cgatcatcag taagagcaaa
    33481 ccatgggttg ttccaggcac attacttgta gcaattgcac tcgttggttt cgttacggca
    33541 ccgaaaaacg ctgtaacagc cgatcaaaaa caactgcagg caaaaatcgc tgaaagtaaa
    33601 aaattagcag aagaaaccag aaacaattca gaaaacacca ctagttcggt tgaccaagcg
    33661 atccttgata agtacgagtt gtccaaggca caagggaata aggcacaaaa attagaacta
    33721 acggcatttg gtgattcagt tatgttggat gctgcaacgg atctacaaga actttatcca
    33781 aaagtagtgg tagatggaga tgtcggacga caattatatg ccagtgtacc gtacattgaa
    33841 aaattaaagg aacaagattt gttgaaagac actgttctga tcggactagg aacgaatggc
    33901 tcattctcgg aagctcagtt tgatagtgtg atggatgcaa tcggtgatcg aaaagtatac
    33961 tgggtcaatg tccgagtacc gacgaagcgc tggcaaaatg acgtgaatgc gatgcttgaa
    34021 cagatgacga aaaaatacga caatatgacg ctgattgatt ggtacagcta cagtaatgac
    34081 catagtgact ggttttatga tgatcaagtc catccaaatc ctgaagggat gacacattac
    34141 attcacttag tcagcgaagc cttgctagga ggaagttcaa gtacgaacag cagtgagaca
    34201 aacgtgacag aatgatagct acaaaaaact tcaggcaaaa acatttgttt ttgcctgaag
    34261 tttttttata tttaccgtgt ttttaacatt catatagatt caggaagcaa aacggttgag
    34321 tgtgtctgta aataacgttc tttttgtttt cgggtgagtt ttttgaatgc agcttctgct
    34381 tttgttgctt cgcttcttga agaaaatact tctgcatgga tcatcttaac aggtcttctt
    34441 ttttgaagac gagtatattt agcaccagta ccattgttat gttcctctaa tcgtctagaa
    34501 agatcggttg tatatccacc ataaaatgtc tgatctttac agaataatac gtaaaaataa
    34561 tgttcatttg ccatagagca tctcacgaat ctctgcagtg tattctccat ccatattgta
    34621 agtgatcaat ggtggagcga ctttgaatcc gtctaatttg ccttctttga ttccttcaac
    34681 taacaaaata tttgcctctt ttccaggttt tgggtaaaca aattggatcc ttttaggtgc
    34741 aattcgatag tcttccatga cctgaatgat ttctaagaac cgatcaggac gatggaccat
    34801 agtaaagcgt ccatttgttt ttagtagctt acttgaaata tcgatcactt cttctaacgt
    34861 cgtatgaatc tcatgacggg cgattgccag gtgtttatta gggttcgttt tatttgttgg
    34921 cagccctttg aaataaggtg gattacagac gactaagtca caagaattat gtcgaacgac
    34981 agataagctg tctttcagat cgatggtgtg gacagtcatt tgttcattta aatggttaag
    35041 cgcgatacta cgtcttgcca tgtctgctag acgaggttga agttcgatag cgtcgatagc
    35101 cgcctgggtt cgtttactta aaaaaagtcc aactgcgcca tttcctgcac aaaggtcaac
    35161 aatcttccct tttttaggta cacgggaaaa gtgagcaagc agcacagcat ctaaagaaaa
    35221 agaaaacact ttggaacttt ggataatctt gatatcttct gcgtataatt ggtcgattcg
    35281 ttcgtcttcg tgaagcatgg attccctcca aaatgaaaaa aatagtcgtc tgtattatac
    35341 tatttttatt aaaaagaacc aaggtggctt gtcaaacctc tattttttat gcatactatg
    35401 accataagaa gggaaaggaa gagactatgt tttatcgatt tatgcgcggg gtagttcgag
    35461 tactattagc cttagttaat ggaaatgccc gctatgagaa taaagaagtt ttaccgcaag
    35521 atgagaatta catactagtt gctccccatc gtacttggtg ggacccatta tatctggcgg
    35581 tagctgcagc acccaaggaa ttcagcttta tggcaaaaga agaactgttc aaaaatccag
    35641 ttctgcgatt tatattaact cgatccaatg cttttccagt taaaagagaa aatccaggac
    35701 caagcgtcat taaaacacct gtaaagattt tgaaatcgac aaatttgagt ttgatcatgt
    35761 ttcccagtgg gacaagacat gctacagaac ttaaaggcgg aatggcattg atttccagaa
    35821 tgtctaaagc gaaaatcgtg ccagctgtat atcaaggtcc gttgacactt ggggatctgt
    35881 tcaagcgtca gagagtgaca gtgcgtttcg gcgaaccaat cgacttatca gatatcaaga
    35941 aaacggataa agaaggtatg gcggagatcg aacgacgaac acaagcagct tttgacaaat
    36001 tggacaaaga agtgaaccct gatttcaaat atgaagtcaa aaaaaaataa aatgataagt
    36061 ttatgcgtgg gcggtctttt gaatctactg tcctgttcag ttcagacttt ttcaatgaaa
    36121 acgagggaga gacgtcaagt ctgccctctt tttgcgttcc aatatgtaaa atagaagaca
    36181 ggtgtcatgt aatggatcaa agtgttataa taattagaag gacatttttt gaggtgattg
    36241 aatgcgtttt ttacatacag ccgattggca tatcggaaaa aaactacaag gttatgactt
    36301 gattgaagac cagcgtcatg tactatcttc tatactagat atcgcgaaag aagaaaaagt
    36361 agaggcaatc gtgattgccg gagacttata tgataggagt gtcccagctg ttgatgccgt
    36421 agaattatac aatgaattga tggtcgattg gaatttaaag gaaaagatcc cgatttttgc
    36481 tatatcagga aatcacgata gcagtacgag actttcagcg ggtacgccat ggttcagtta
    36541 ttcgaactat tttttgcgaa cacagctagc agaagcattt gaaccagtcg agtttaaaaa
    36601 cgtacaattt ttctttcttc cttactttga accaattgca gcgagattat attttgaaga
    36661 tgacaatatc cgaacgatca agcaagcggt agccaaggtg atagataaaa tgcaggagct
    36721 atttgattca gaaaaaaaac aagtattgat tagccatttt tttgtaatgg gaagtgtaaa
    36781 atctgattcg gaaacgaaaa tcgaagtagg cgggttggat tcgatccctg gtgaactact
    36841 tacaccgttt gattatgtag cactaggaca tttgcataat caagctgctt tgagacagga
    36901 taatgctcgc tatagcggtt ccccattgaa attctcttta tctgagatca accaaaaaaa
    36961 aggcgtatgg atcgttgacc tgacaacagg cctggagttg aattttaggg agatcgaacc
    37021 cctgcatgat attgaagaaa taacagcgag tttcaaagaa ttgctggatc ctgcctttta
    37081 tcagtccgtc aatcgggaga attacatcta tatctatctc actgatagaa cgatcatccc
    37141 gaatatgatg aaccagcttc ggcaaatata tcctcgtatt ttggggattg aacgggcaaa
    37201 cggtcgagca gaacaaacaa agcgcaaacg aaaagtacta gaaatgaaag atcctcaaaa
    37261 attagcagaa gcatttttta gtgaaatgac ggaagagcag ctaactgcaa accaagaaaa
    37321 atggctcgga gaaacgttgc aagaaatcat ggaaatggaa tgatcaagaa tgaaaccgaa
    37381 aaaattacaa cttaaaaatt ttggtccttt tatcaatgag accgtagatt tttcacgttt
    37441 gacagaagca ccgctttttt tgatcagcgg gaagacagga gctggcaaaa cgacgatttt
    37501 tgatgggatc accttcgcac tttttggtga aacttccgga agacttcgct ctggaaaaga
    37561 gatgcgttct ttgtttgcta caccagacga agaaacaagc gtgacttttt cttttgaaca
    37621 tcagcagatg agctatgaaa tcgaacgaaa accagaacaa gttttagcaa aacgaaaagg
    37681 aaacggaaca aaaaaacagg cagccaaagt aacgttgacc atttttgatg ggaaaggaaa
    37741 agaaataaaa caaatcacaa aacgtacaga agcagatcag ttgatcagag aactgatgaa
    37801 tctcgatgcg aaacagtttt ctcaaatcgt tttactaccc caaggagaat tccgtaattt
    37861 tttggtttca tcaagcagtg ataaagagac tgttttacgt catttattcg gtactcaatt
    37921 tttccaacag ttcaatgagc gattaaaaga aaaagcaaaa caacagcaac aaaaattgga
    37981 tcatttagag caagagctgc atcttctgca aaaaaggttt gttttagtca aagaagagga
    38041 accagcagca gggtttgaag ccgttttagc ccaatggaca aaccatcagg caatactcga
    38101 aaaacaaatc acggaagaaa aagaagctct aacggaactt caggaaaaac aaaagcaact
    38161 agaaaattcc tactatcgtt ttgaaagctt gaaaaaaagt tatgaagaaa aaacgatatt
    38221 aataaacaag caaaaggaat tagctgctca aaaagacgaa atagaggaaa agaaacgatg
    38281 gattgggtat tatgaattta cgcaacgatt agtcgaacca atccagcgcc tacaagaaat
    38341 aaaagttgaa aaaatagaac tgtcacaaaa aagtagtgag caaacaaaga aactagcgat
    38401 gactacgcag aattatgaag agtggcaaaa aaatgaagca aaccgcgtac gccgacaaac
    38461 aaaaatcaaa gaagctgctc aaaaactgca aaacaaacag atgatggtgc caattgtcga
    38521 atccttacgt agtaaaaaac aagacgcaga acaattaaca atttcattgg ataaagaaga
    38581 attgaatgcc gagaagctgt atcaaaatct cgctatctat caggaaagaa ctcgaattct
    38641 tcaagaaaat ttactggatc aggaaaaact gcaagaagat cgtcttgctt ttggacagtt
    38701 aaaatatcta gatgaaaagc agcaagaaat agaaaaacaa aaagcaaaag acactgggtc
    38761 gctcgcacag ataagagaag aactcaaaaa agcaactgaa gaaataacaa aaaatagtca
    38821 gctattgaaa gaacagacag aacaagcaga acagctgaaa agccaatggg caaagcagca
    38881 aattgctcgt cttcagcttt ttttgcgtcc aggggagcca tgtcttgttt gtggatcaac
    38941 ggaacacccc atacaacaat taaaacacga acagccaaat aaggaagata tcttaaaagt
    39001 agaacaacaa ctggcagaag ctgagtctgc tgtagaaaga actcagcaaa tcattgccaa
    39061 aagtgaagca catgaaaata atcttcaaca acaattatca gatttagaac aaaaaatcat
    39121 ggcaacgaat atacaattgg ctgaacaaga agaacacttg caacaggctt tgaaaaaggc
    39181 atttcctgag ataacagaac aaaaaacaaa aacaactctt gtagtaatcg aacaatcttt
    39241 agatagacga aaaagcaaga tggccaatgc aagaatagag ctggaagaaa taagacaaca
    39301 agcagaaaag ctcgaacaac aattagctgt ctacaaacag cagcagacag aaaaaaataa
    39361 tcagctccag caactgcaag gagaaatcaa aagtttagaa caacaattgc aagatacgga
    39421 tagtaactgt ttagaagagg agatacgacg acttgaaaaa cagttagaag aagactcgaa
    39481 tcaactagct gttgagcaga aagcaggagt gcaattgttg caagatcttc atacgcttcg
    39541 gacacagctt gatcttcaac atgaacaaat agaaaaaata gatgaaaaaa tagaaaagga
    39601 acaagaaaga atagctaaaa agttagaaag ccagtcgtat ttttcaacaa ttgaagaaat
    39661 ctgtgcattt gcacaacagc aagcacaata taaagaaaac caacgtacag ttacttctta
    39721 tcatgaagag caattagtga cagctgaccg gctgcgtcaa ttagaaaata tccagctaac
    39781 tgagacattc ccagatacag aagagatcag ccaacagtta gaagaaataa gacgtatgat
    39841 cgaaataaag caagaaaaga tatacggttt acaagaacaa aaaattagta atcaaaaaat
    39901 ctacgatgaa tgtctttcga tttatcacac aagccagacg aaattagatg agcttacgca
    39961 attacagcag ctttcgcaaa cattcaacgg agaaaatcct aagaagacta gtttggaacg
    40021 gtatgtgctt caggtttatc ttcaagaagt gttacaagta gcaaatgatc atctccaacg
    40081 tttaacgaaa aatcgttatc aatttgagct agcggatacc attggcagtt atcgagggaa
    40141 aaccggatta gaaatcaata tctatgatga tcaggcaggt atgtctcgaa gtgcgcatac
    40201 cctgtctggc ggtgagagtt ttattgcagc actttcttta gcattagctc ttgccgatgt
    40261 gatccagaca caagctggtg gtgtagcaat cgatgcattg tttattgatg aaggatttgg
    40321 gtcattagat gaagaagctt tggaaatggc aatggaagct ttagaaacga tcgaaagtga
    40381 aggaagaatg atcggcatca ttagtcatgt aagagaattg aaagaacgag tattacaaca
    40441 aataagaatt gatacagaag gtagcggaca aagcaaaata cgctatcaac ttggataaaa
    40501 agggagattg gtaactatga aatggagtat tccagaaaaa gtcattgcac gtggacgtca
    40561 atacttgcaa gaaggccgag tgttatcggt tgtccctgac caagaagaaa acgtgtggca
    40621 tgcagaagtc ttgggcagtg aattgtatat ggttgatttg gatggtacag caaaagagat
    40681 agattactgc caatgtccct attgggagga acataaatat tgtaagcata cagttgctgt
    40741 ggaactttat ttacggtcga aaaatctatc tcgtatcatg aaaaaagacc agccaatgct
    40801 agaaccggta aaagcaacaa gtgagggaga aatcattgca aaaggttttt ctcgtctaaa
    40861 aacacaaaac aatcctcaag cggtgcaacc tttgattatt gaataccaag tagaaacgat
    40921 tgagactaat cagtatcacc cagagctttc tattttggca ctttacttgc gtatcggttc
    40981 attggcatca ccgaaaaaaa catatatcgt aaaaaacatc tatgattttt tacaagctta
    41041 cacagaaaaa gaaacttaca ctgttaataa acagtaccag tttcgtctag ataaacgggc
    41101 gtttcaaaaa gaagatcaaa aaatactcac gcatcttgca ggaagtgctc agacgcatca
    41161 attattaggt acgaacggct tacaagtcaa aggaaaacta gataaaagat atctgcttct
    41221 cccagtagaa caagcccgct tcctgttaga gcagatgaat cagactacac gtttgcatct
    41281 gcagataaat gaacaaaacg atcaaggaat acaattttcc gatcaaaaca aacctttatc
    41341 attcaaagtg gaaaaatttg gagaagatta tttgctgcaa gcattgaatg attttgattt
    41401 tttctttatg cattatcagt ggggagttat ccaagggaca ttttattctc tgacgaaata
    41461 tcaacaaacg gtttatcaaa cttggaaaca attgattcgc cgattggaga aaccagaagt
    41521 agtgttcaag aaaaaagaat taccgtcttt gtttaaagag attatccctc tgctgtcaga
    41581 gatcggcgac gtagctattg cccctgcagt agaagaggaa gtagcagata tccccgtcga
    41641 atttattttc tttttccgaa aagcaaaagg aaaaatccaa gctcgagtcg atttcgtcta
    41701 tggcgaagtc atctattcga ctgacccgaa gcatgaggtc tcgacagaac aggcttttca
    41761 ggtgttacgt gacacagtag aagagaaacg agtacttgat ctattcagaa tgtatcgcta
    41821 ccagaaaaat gaaactggtt atgaacgtcc attgccaagc ggggaagaac tatatgcctt
    41881 tttccgcaca gaactatctg tcttccgtca gtttggtgaa gtcagacttg gaagaaaatt
    41941 gcgagagttg tatttagatg cgcataaaca tcaaccgaaa atcgaagtaa ccgagaccgg
    42001 ttcatggctc gatattcgat tcgatgtgac agggatagag gagcaagaaa tcgatcatgt
    42061 cctgcaaagc ttgctgagaa atgatgcttt ttatacatta gaaaatggtc aggttctttc
    42121 ttttgattca gaagaatttc aacaaacaag ccaagtgttg caacaattac gggaatcgat
    42181 acgtacagaa gaaggaacga tccatgtacc taaaaatcaa ggattgatca ttcaaaatca
    42241 attggaaaaa agcaatgcta cttttagtga atcgttccaa acgatggtac aagatctcat
    42301 tcatccagaa agataccagg cacagcttcc caaaggattg aatgcaacga tgagagatta
    42361 tcaaaagcaa ggttttcgat ggttgaagat gttaggtcat tatcagtttg gcggtatttt
    42421 agccgatgaa atgggacttg ggaaaacact acagacaatt gcctttttac tttcggaaaa
    42481 agaagaacgg aaaagcttct ctgctttgat cgtagcgcct gcgagcttaa tttacaactg
    42541 gcaggcagag gtgagaaaat tcgccccttc tttaagtatc caagtaatca atgggaataa
    42601 aaaagaacgt gaagaattac tggctaaaga cacagatatc cgagtgactt cttatgccag
    42661 cttaagacag gatctagcta attatcagtc gcaaaaaatt gattatttga ttcttgatga
    42721 ggcacaaatg gtcaaaaata gcagtacgaa aactgcacaa gcattgagag aattagctgt
    42781 acctcagcgg tttgcactga gtgggacacc aatcgagaat aatttagaag aattgtggtc
    42841 attgtttgca acgattatgc caggattttt cccgactaaa acaaaattca gggaacttgc
    42901 cgcagaagaa attgcgcaaa tgattcgacc gtttatttta cggcgagata aacaaactgt
    42961 gttgaaagac ttgccggaaa aaacagagat gaatctatat tctgcactta cagaagaaca
    43021 aaaaactgtt tatttagctt acttgcgtca aatgcgtgaa gaaatcacat caatggattc
    43081 tgaagcattc aagaaaaacc ggatcgggat acttgctggt ctgacaagac tgcgacaaat
    43141 ttgctgcgat cctcgtctgt ttattgaaga ttaccaaggc ggttccggca agctcgaaca
    43201 agtaaaagat ctactggtag ctgccaaaga gaacaaacga agagttttgc tgttttctca
    43261 atttacaggg atgctgacta tcttgcaaga agagttagcc gagctaggga tctcgacatt
    43321 ttatttaaga ggcagtacga aaccacaaga tcggttaagt atggtagacg catttaatgc
    43381 tggcgaaaaa gatgtttttc tgatttcctt aaaggctggg ggaactggat tgaatctcac
    43441 gggagcagat acggtcatct tgtatgacct ttggtggaat cctgccattg aagaacaagc
    43501 tgctggacgt gcacatcgga tcggacaaaa aaatgtggta gaagtgtggc gtatgatctc
    43561 tgaaggaacg attgaggaac gcatggattc actccagcaa gaaaaacgcg aactgttcca
    43621 aaaagtgatc caaggtaatg aagaacagtt gacgaaactg actgaagaag acatccgcat
    43681 gattttaagt gtaggagaaa tcgaggagta agagactcga aaaagcgttc agtaatgggt
    43741 aagaaagcct ttctaaagaa gcgattgttt atttgattta aaaaatagca aggaggctgg
    43801 gattccattt tatcccagct tccttgctat cattttttta tttctatttt taaaggttat
    43861 acaaagattg tcttcattgt ctctaacagc tgatcaaaag aagaaatctc gatatcgcaa
    43921 attggagtct gttcaatttt gcgttctcga tggttgaacc aaagcgcttg ccagttagca
    43981 ctttttgcac ctagaacatc attgtcgtaa ttatcgccaa cgtataaggt ctcttctgga
    44041 agcatatgaa attctttctc agctagctga aaaatctctt tttctggttt ttggaaacct
    44101 gttgcttgag aaatgagcat attgcgggaa ggaatccaat tgttcaattg aagttgcttc
    44161 agtttttttg tctgatggtc agtcggccca tttgtgataa ttcctaatgg gacattcttt
    44221 tccttcaaga aatcgagggt ttttttgacc tcctcatgca agcagatatt atcaagttct
    44281 tcctcgtaaa ttttttgaaa taagaggcca tcttcttccg taatatgagg gtagtctaaa
    44341 tcttttagtg attggctgat ccgatgtgct cgcatatatt ctagtgtcca atctcctgcc
    44401 attacttttg ggaagttttc atcactatga tggcggaacc ggatatatag tggatgcata
    44461 tctgcatctg aaacaagcgg aacgactctt ttgaccgcgt ttcgaaatgg ctgctgctga
    44521 tcatacatcg tatcatcaac atcaaacaca actgatttaa tcaaaatttt acacgccttt
    44581 cggttttcat tgattttttc acaaatcctt gactattgta acagaaaaaa ttgtctaaaa
    44641 aagctgataa ataaaggttt ttcttactat atttctattt ccttgacaga ataaggtttt
    44701 tatatttcag tttttttcac taaaaatcgt gaaacacgtc aataagtttg atgaacaaat
    44761 taatcattat ggtagaagtc gattgattta ttttaatgca atctctgtgg aattttcgaa
    44821 ag
//
LOCUS       DAYYGS010000013        42623 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000013 DAYYGS010000000
VERSION     DAYYGS010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.013"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            83..2680
                     /gene="adhE"
                     /locus_tag="ACHA44_000671"
                     /gene_synonym="adhC"
     CDS             83..2680
                     /gene="adhE"
                     /locus_tag="ACHA44_000671"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFM8903986.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            2909..4015
                     /locus_tag="ACHA44_000672"
     CDS             2909..4015
                     /locus_tag="ACHA44_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8903987.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            4032..5060
                     /locus_tag="ACHA44_000673"
     CDS             4032..5060
                     /locus_tag="ACHA44_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFM8903988.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            5066..5545
                     /locus_tag="ACHA44_000674"
     CDS             5066..5545
                     /locus_tag="ACHA44_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFM8903989.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            5561..6856
                     /locus_tag="ACHA44_000675"
     CDS             5561..6856
                     /locus_tag="ACHA44_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFM8903990.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            6867..7691
                     /locus_tag="ACHA44_000676"
     CDS             6867..7691
                     /locus_tag="ACHA44_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8903991.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            7856..8755
                     /locus_tag="ACHA44_000677"
     CDS             7856..8755
                     /locus_tag="ACHA44_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8903992.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(8881..9750)
                     /locus_tag="ACHA44_000678"
     CDS             complement(8881..9750)
                     /locus_tag="ACHA44_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8903993.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            9903..11363
                     /gene="rhaB"
                     /locus_tag="ACHA44_000679"
     CDS             9903..11363
                     /gene="rhaB"
                     /locus_tag="ACHA44_000679"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFM8903994.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            11360..12616
                     /gene="rhaA"
                     /locus_tag="ACHA44_000680"
     CDS             11360..12616
                     /gene="rhaA"
                     /locus_tag="ACHA44_000680"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFM8903995.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            12630..13475
                     /gene="rhaD"
                     /locus_tag="ACHA44_000681"
     CDS             12630..13475
                     /gene="rhaD"
                     /locus_tag="ACHA44_000681"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008994 - rhamnulose-1-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFM8903996.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            13477..13788
                     /gene="rhaM"
                     /locus_tag="ACHA44_000682"
     CDS             13477..13788
                     /gene="rhaM"
                     /locus_tag="ACHA44_000682"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFM8903997.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            13870..15021
                     /gene="fucO"
                     /locus_tag="ACHA44_000683"
     CDS             13870..15021
                     /gene="fucO"
                     /locus_tag="ACHA44_000683"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008912 - lactaldehyde reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFM8903998.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            15148..15906
                     /locus_tag="ACHA44_000684"
     CDS             15148..15906
                     /locus_tag="ACHA44_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFM8903999.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            16006..16650
                     /locus_tag="ACHA44_000685"
     CDS             16006..16650
                     /locus_tag="ACHA44_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFM8904000.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            16657..17664
                     /locus_tag="ACHA44_000686"
     CDS             16657..17664
                     /locus_tag="ACHA44_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFM8904001.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            17680..18522
                     /gene="kduI"
                     /locus_tag="ACHA44_000687"
     CDS             17680..18522
                     /gene="kduI"
                     /locus_tag="ACHA44_000687"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFM8904002.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            18542..19345
                     /locus_tag="ACHA44_000688"
     CDS             18542..19345
                     /locus_tag="ACHA44_000688"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFM8904003.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(19594..20658)
                     /gene="fni"
                     /locus_tag="ACHA44_000689"
     CDS             complement(19594..20658)
                     /gene="fni"
                     /locus_tag="ACHA44_000689"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFM8904004.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20655..21740)
                     /locus_tag="ACHA44_000690"
     CDS             complement(20655..21740)
                     /locus_tag="ACHA44_000690"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HFM8904005.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(21753..22730)
                     /gene="mvaD"
                     /locus_tag="ACHA44_000691"
     CDS             complement(21753..22730)
                     /gene="mvaD"
                     /locus_tag="ACHA44_000691"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HFM8904006.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(22723..23667)
                     /gene="mvk"
                     /locus_tag="ACHA44_000692"
     CDS             complement(22723..23667)
                     /gene="mvk"
                     /locus_tag="ACHA44_000692"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HFM8904007.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            23983..24642
                     /locus_tag="ACHA44_000693"
     CDS             23983..24642
                     /locus_tag="ACHA44_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8904008.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            24727..25632
                     /locus_tag="ACHA44_000694"
     CDS             24727..25632
                     /locus_tag="ACHA44_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8904009.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(25634..26266)
                     /locus_tag="ACHA44_000695"
     CDS             complement(25634..26266)
                     /locus_tag="ACHA44_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFM8904010.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    26417..26542
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            26586..27110
                     /gene="infC"
                     /locus_tag="ACHA44_000696"
     CDS             26586..27110
                     /gene="infC"
                     /locus_tag="ACHA44_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFM8904011.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            27182..27382
                     /gene="rpmI"
                     /locus_tag="ACHA44_000697"
     CDS             27182..27382
                     /gene="rpmI"
                     /locus_tag="ACHA44_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFM8904012.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            27435..27794
                     /gene="rplT"
                     /locus_tag="ACHA44_000698"
     CDS             27435..27794
                     /gene="rplT"
                     /locus_tag="ACHA44_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFM8904013.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            28046..29383
                     /locus_tag="ACHA44_000699"
     CDS             28046..29383
                     /locus_tag="ACHA44_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HFM8904014.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            29431..31560
                     /locus_tag="ACHA44_000700"
     CDS             29431..31560
                     /locus_tag="ACHA44_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HFM8904015.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            31535..32224
                     /locus_tag="ACHA44_000701"
     CDS             31535..32224
                     /locus_tag="ACHA44_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904016.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(32632..33309)
                     /locus_tag="ACHA44_000702"
     CDS             complement(32632..33309)
                     /locus_tag="ACHA44_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8904017.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTRFRWLR
                     LTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDYFED
                     IQDRPIK"
     gene            complement(33445..33639)
                     /locus_tag="ACHA44_000703"
     CDS             complement(33445..33639)
                     /locus_tag="ACHA44_000703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904018.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(33689..34129)
                     /locus_tag="ACHA44_000704"
     CDS             complement(33689..34129)
                     /locus_tag="ACHA44_000704"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFM8904019.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(34133..34978)
                     /locus_tag="ACHA44_000705"
     CDS             complement(34133..34978)
                     /locus_tag="ACHA44_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFM8904020.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            35127..36545
                     /gene="pepV"
                     /locus_tag="ACHA44_000706"
     CDS             35127..36545
                     /gene="pepV"
                     /locus_tag="ACHA44_000706"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HFM8904021.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(36602..37549)
                     /locus_tag="ACHA44_000707"
     CDS             complement(36602..37549)
                     /locus_tag="ACHA44_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904022.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(37772..38122)
                     /locus_tag="ACHA44_000708"
     CDS             complement(37772..38122)
                     /locus_tag="ACHA44_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8904023.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            38322..39401
                     /gene="pepA"
                     /locus_tag="ACHA44_000709"
     CDS             38322..39401
                     /gene="pepA"
                     /locus_tag="ACHA44_000709"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HFM8904024.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            39545..39865
                     /locus_tag="ACHA44_000710"
     CDS             39545..39865
                     /locus_tag="ACHA44_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8904025.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            39887..40351
                     /locus_tag="ACHA44_000711"
     CDS             39887..40351
                     /locus_tag="ACHA44_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8904026.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            40556..41161
                     /gene="ytpR"
                     /locus_tag="ACHA44_000712"
     CDS             40556..41161
                     /gene="ytpR"
                     /locus_tag="ACHA44_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HFM8904027.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(41200..42489)
                     /locus_tag="ACHA44_000713"
     CDS             complement(41200..42489)
                     /locus_tag="ACHA44_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HFM8904028.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
BASE COUNT        13406 a         7806 c         9150 g        12261 t
ORIGIN      
        1 gtgtttacat agaaaaataa atattgtatg atatacatgt gaattattta acaaataacc
       61 atgacccatt aggaggataa tgatggctaa agtaatagaa aaagaaaaaa cgaagacaat
      121 tgacgtacaa gcaatgattg atgatttggc agaaaaagca aatgcagcat tgaaagaaat
      181 ggaaagcttt gatcaagaaa aagtagacca tatcgtgcat gaaatggcaa tggcagcttt
      241 ggatcaacat atgccattag cgaagatggc tgtagaggaa acaggccgcg gtgtttacga
      301 agataaagca atcaaaaata tgtatgcgtc cgaatatatt tggcataata tcaaacatga
      361 taaaacagtt ggtgtgatca atgaagatgt ccaaaaaggg ttgattgaaa ttgctgaacc
      421 agtcggtgtg gtttgcggag taactccaac aacgaatccg acttccacaa cgattttcaa
      481 atcaatgatc gcgttgaaaa caagaaatcc aatcgttttt gctttccatc caagtgccca
      541 aaaatcttct gcagaagcag cacgagtggt acgtgatgct gctatcgctg ccggagctcc
      601 tgaaaattgt atccaatgga tcgaacatcc ttcgatcgaa gcgacttcta tgctaatgaa
      661 ccatccaggt atcgcgatcg tcttagcgac aggtggagct ggaatggtca agtctgctta
      721 ctctacagga aaaccagctt taggtgtagg tccaggtaac gtaccagctt atattgaaaa
      781 aacagcaaaa attaaacgtg cagtgaacga tttgatcgtt tcgaaaacat ttgataatgg
      841 aatgatttgt gcttctgaac aagctgtgat cgtggataaa gaaatctatg cagcagtaaa
      901 agcagaattc caagcacacc aagtttatat cgtaaaacca gatgaattgc aaaaattaga
      961 agatgcagtg atgaacgaag gaaaatatgc ggtcaatcca tcaatcgttg gacattctgc
     1021 gatggaaatc gcaaaattgg caggaatcag tgtaccaaaa ggaacgaaaa tgctgattgc
     1081 tgaattggaa ggcgtaggcc cagattatcc gctatcaaga gaaaaacttt ctccagttct
     1141 tgccatgatc aaagctaaca acacggacca tgcgtttgat ttgtgtgaag gtatgttgaa
     1201 tcttggagga ttaggacata cagcagttat ccattcagaa aacgaagagt tgcatgtcaa
     1261 attcggtctt cgaatgaaag cttgtcgtat tttagtgaat acaccatctg cagaaggcgg
     1321 catcggtgat atctataacg aaatgattcc gtcattgact ttgggatgcg gttcttatgg
     1381 taagaactct gtttctcgta acgtctctgc tgtaaactta atcaatgtca aaactgtagc
     1441 gaaacggaga aataacatgc aatggttcaa attaccgcca aaaatcttct ttgaaaagaa
     1501 ctctctattg tacttagaga aaatggaaaa cgttgaacgt gtcatgatcg tttgtgaccc
     1561 tggtatggtc caattcggtt actgcgatac agttcgtgaa gtattggcac gacgtaaaaa
     1621 tgatgtgaag atcgaagtct tctcagatgt tgaaccgaac ccatcgacaa ataccgtata
     1681 cgcaggaacg aaactgatgg cagacttcaa gccagataca gtaatcgctc ttggaggcgg
     1741 ttctgcaatg gacgcagcta agggaatgtg gatgttctat gaacacccag acacatcttt
     1801 ctttggtgca aaacaaaaat tcttggatat ccgtaaacgg acatataaga tcgacaaacc
     1861 agaaaaaaca caattcgtat gtatcccgac aacatcaggg actggttctg aagtaacgcc
     1921 atttgcggtc atcactgaca gtgaaacaca tgtgaaatat ccgttagcag attatgcatt
     1981 gacgccagat gtcgcaatcg tcgatcctca attcgtcatg tcagttccag catctgtcac
     2041 agcagatacc gggatggatg tgttaacaca tgcaatcgaa tcctatgttt cagtcatggc
     2101 ttcagattat acacgtggat tgagcctaca agcaatcaag ttagtctttg actatttaga
     2161 aaaatcagta aaaacacctg atatggaatc acgtgaaaaa atgcacaacg cttctacaat
     2221 ggctggtatg gcgtttgcta acgcattcct aggtatttgt cactcagtcg cacataaaat
     2281 cggcggggaa tacgggatcc ctcatggacg gacaaatgcg attcttttac cgcatattat
     2341 ccgctataat gcaaaagatc cacaaaaaca tgcgatgttc cctaaatatg actatttccg
     2401 tgcagataca gattacgcag atatcgcaaa attcttaggt ctgaaaggcg aaacaacaga
     2461 ggaattagtc gaagctttag caacggctgt ttatgaatta ggaaaatctg tcggcatcaa
     2521 catgagtcta aaagctcaag gagtcacaca agaaacacta gatacaaccg tagaccgtat
     2581 ggctgaatta gcttacgaag accaatgtac gacagctaat ccgaaagaac cattgatctc
     2641 tgaattgaaa cagatcatca ttgatgctta caacggataa gcaaaagaaa gcgtagaaaa
     2701 aagataagaa aatagatgaa aaagaacttg gaaaacgttg tttctccaag ttcttttttt
     2761 aatttccaag taactgaaag ttacttttgt tatttaacat ttaggtcaat tttagacagt
     2821 ttttgttcaa aaagcgatat ttatagtgga cggtattact gctatattaa ggatataaaa
     2881 tgaaagcgtt taaaaaggtg gcgtaaagat gattattaaa gaagagattt ttgtacctaa
     2941 agaattagtg aaagaaaaaa ttgacctgct cgttgaaaac ctgacaacga tcaaagacga
     3001 taatggtgaa tttttgttgg actttgatgg actgaaagta gatgacaaaa gctggacagt
     3061 gtggaattgg ccacaaggtg tcggtctata tggtatttat aaaaattacc agatgacaaa
     3121 aaatcctcgc gcttatcaag tggtcaatga atggtttgag gatcgtatgg aagaaggcgc
     3181 tccgcccaaa aacgtcaata caatggcacc gctattgact atggcttact tgtatgaaga
     3241 cacgaaagat tcacgctatt tgccttattt agaacaatgg gcagaatggg tcatgaatga
     3301 catgcctcgg acaaatgaag acgggttaca gcatgccaca tatggaccgg aaaataagaa
     3361 tcagttgtgg gacgatacat tgatgatgac agtgcttcct ttggcaaaaa tcgggaaact
     3421 gttgaatcgc ccagaatatt tagaagaagc tcgttaccag tttatgatcc atatcaaata
     3481 cttgatggat aaacgaacag gattgtggta tcacggctgg acgtttgaag gaaatcataa
     3541 ctatgccgaa gcattttggg ctcgtggaaa ctgctggttg acgattgcta ttccagagat
     3601 cattgaaatc ttagaattat cgaaagaaga tgcattgcgc aaattattga tcgaaacact
     3661 tgaagcacaa gtgcgtgcat taaaaactta tcagtctgaa agcggtttat ggcatacgtt
     3721 gttggatgat ccttcttctt acgtagaatc ttctgcaact gcaggatttg cttacggtat
     3781 tttgaaagcg gtccataaac gttatttacc acaagaatat aaagaagtcg cttatcgtgc
     3841 tatccaagga ttgcttgaac aaatcgatga aaaaggagaa gttcaaaacg tttcaatcgg
     3901 gacaggaatg ggggacagtt tagattttta tcggaatatc ggaatcacag caatgcctta
     3961 tggacagtcg ttaacggtgc tttgtttgac agaattgctc gtctcttatt gttaaaagaa
     4021 aggaaaccca tatgaaaaaa acatcactgc tgtttgcagc aagttcgctt agcctaggtc
     4081 tgctaggtct aagcggctgc ggaaataatt ctagtgtggc aactgtcgca gatgcagatg
     4141 taaatgagat agttaccttg cgctttgctt atgcaagcaa cagccagcca gtaattgatg
     4201 caatgaatga atttgggcgt ttagtaaaag aaaaaacaga tggttccgta cagatccagt
     4261 atttcccaga tggacaactg ggtggagaac gtgaactgat cgaattgaca cagtcaggag
     4321 cagtagattt cacaaaagta agtgcctcag cgttggaaag tttctctaaa gattattcga
     4381 ttttttcagt tccttatcta ttcaatgatg aagaacattt ctttaaggtg atggataatg
     4441 aagaaataat ggagccaatc taccaatcga ccaaaaattt aggatttacc ggattgacct
     4501 attatgattc tggtcagcgc agcttttata tggtagacgg tccgatccat acgccggaag
     4561 atttgaaggg aaagaagatc cgtgtgatgc aaagcgaaac cgctatcaaa atggtagagc
     4621 ttttaggcgg ttcgcccgta cctatgggaa gtgatgaagt gtacacgtct cttcaatcca
     4681 atttgatcaa cggatcagaa aacaatgaat ttgtcttgta tacagctgga catggtggtg
     4741 tggctaaata ctattcctat gatgagcata cacgtgtgcc agatatcatc atcatgaatg
     4801 atgccatcaa agaacagcta actgatgagc aggaaaaagc cattgaagaa gcagcgaaag
     4861 aatcaacggt ttttgaaatt gaagcttttg caaaagcaat tgaagaagaa aaagctttag
     4921 cacaagaaga acatggagtc caattcaatg atgtagataa tacaccgttt cgagaggcag
     4981 ttgccccact tcatgatata ttcaaaaacg atcctaccta caaagacctt tatcaaaaga
     5041 tccaagagca aggagcgtag ctgtgatgaa aatgatttta aacaaggtat tagagatcct
     5101 gggagctgtt ttgcttgttg ggatggtcgc tgttgtttta tggcaagtat tttccagaac
     5161 ggttttagga aatccaaata cggtcacgga agagcttgtt cgattcggtc ttgtctggtt
     5221 tgccatgttg tcttccgcgt atgttgtggg acaaaaagca catttagccg taacggtttt
     5281 aagtgaaaaa ttaactggca aaaaaagtaa aattttagaa gttgttgttc aaagtttatt
     5341 tctggtattt gctgcgatga tcatggtgta tggcggatgg aatgctgtca cattgacaat
     5401 ggggcagatc tcgccttctt taagtattcc tatgggatat gtttatctgt cagtacctgt
     5461 atcgggggta ctcatcatta tctatagcct gatcaatcta atcgatacat taagcaacaa
     5521 gccagcaatt caagaaaaaa tataaaaatg gaggaataaa atggcaatag aagccggatt
     5581 agtgttattt tttgtatttg ttgtgttgct tgtattaggg atgccgattg ctataagtat
     5641 tgcattctca tcgatgatga ctttattgtt agttataccg tttgatgttt ctgcttttag
     5701 ttcagctcaa aaaatggtgg gaagtatcaa tagtttttcg ctgatcgcta ttccgttttt
     5761 cgttctctcg ggcattatca tgaataatgg agggatagct aagaaactag tggattttgc
     5821 gatgttgttc gtcgggcgcg tgccgggggc tttggctcat acgaatgtgt tagggaatgc
     5881 tttatttggt tccatctcta gctcggccat tgccgcttct accgcgattg gcggagtgtt
     5941 gatccctcag caagtggaag aaggatatga ccggaagttt gcgacagcgg tcaatatcgc
     6001 gtcagctccg acaggtatgg tcattcctcc aagtactgct tttatcatgt attcacttgt
     6061 tgctggtggt gcttcgatct ctgctttgtt tatgggaggt tatctcgtag gcgctttgtg
     6121 ggcgctagga atcatgctag tagcattcat cgttgctaaa aagaataaat accctgttgt
     6181 cgaaaaagga cagatggctc atgctggaaa aataataaaa gaggcgattc ctagccttct
     6241 attgattgtc atcattatcg gtggtatctt gacggggata tttacagcag tagaagcttc
     6301 agcgatagct gtcatttact ccctgttgat tgctatgttt tactataaaa cggtaaaact
     6361 aaaagatata cctaatatgt tgtttcaatc agtagcaatg tcagggacga tcatgttctt
     6421 attggctact tcttccatga tgtctttcgc catggcgttt accggtattc ctcaggcaat
     6481 tagttcgttg attatgggcg taacggataa taaatttatt attctgttat tagtcaatct
     6541 tgtgttatta ctggtgggta tgtttatgga tgtggcgcct gctatcttga tttttacacc
     6601 aatattttta ccaatcgtca caagtgtagg ggttgatcct gtacattacg ggttgttttc
     6661 aatcatgaat ctgtgtgtag gctccatcac gccgccagta ggtacaggac tatatgtcgg
     6721 tgcaagtgta ggaggcgtaa aagcagaggc aatgctgaaa ccgcttcttc ctttttatgg
     6781 ggttatctta gtcgtcttgt ttttgattac ttacttcccg caaatcgtca tgtggctacc
     6841 caatatgctg agttaggaga atagttatgt tgaacattac aattagagga catgatcttt
     6901 ccgaagtcca aacgatagaa gatcttgcag aaaaaacaaa agaacaaggg attcatacac
     6961 tgcagctcgc cttaggtctt tcgtttcctg aaataccttc tggcgcaaat gagatcaatt
     7021 ctgggatggg aaattatgtc aaacgagttt tagaaaagca agaagtgacc gtaggaattt
     7081 taagttgcta tatcaatatg attcatccag atttaacgat acgacaagag ttgctgacaa
     7141 agttcgagca gtatcttcgc tatgcttctt cctttggtgc gtcgatggtt gcttcggaaa
     7201 ctggatgtgt tttgccggaa atccaatata cggaagaaaa tttcacagat gaagcatttg
     7261 ctgaagctgt gtccgttatc cgtcgcttgg taaaagctgg agaaaaatac caaatgatgg
     7321 tggggataga accaggtttg aatcatccgg tgtattcttt agcaagggta gagcagctga
     7381 tccaagcagt agattcggac tatctaggaa tcattttaga tccgaccaat ttgatcacaa
     7441 gtagtgatta tcaagaacaa gtccaattgg tagaagaagc ttttgaacgt tttggtgaaa
     7501 agatatgtgc ggttcatttg aaagattttc gtgtcgaaca ggaaaaaatc gtgccggtaa
     7561 atttgggaga cggtgtgatc gagtacacaa aaatcaaaga aatcatcatg aagaatcgtc
     7621 cgtacctgta tgtggttctt gaagaaacaa aagatgatgg gattcgttat ggaagaagtc
     7681 ttcttgaata atgtaaagaa gacgttggtt aactaatatt tagaatggag ggggcggggc
     7741 tagaagatgc cacgtctctt cttttttata agagtatata aaagcagctc tttttgatta
     7801 caaaaaacgt ggtataataa aattgtgcat aagtgaacaa aaggaggtgc ggcacatgct
     7861 ccaaaagaaa atcagcggtg aacaagtcaa tacgatggtt tctgatcagt atgaaatcta
     7921 tcatgtcaaa gatgtcgtca gccaaaataa aacgatctat catcatcatg atttctatga
     7981 gatccatgct acattaaagg gagaagcgac tttttatttg aacggacgac aatttacgat
     8041 caaagcagga agtgtgctgc tcatccattc caatgatttg catcgtatcg tccgccaatc
     8101 ttcagatgta ttcgagcgtg tctatatctt tgtcacccct tcttttctag aaagtcgttc
     8161 cacgccatgg tctaatctat cggcttgttt tcaaccaatg ggagagaaga ggagtcgtgt
     8221 attgcaaata gaacaggaag tattgaagca aaaattatca tttatcgacc atcctcctga
     8281 taaaaaaatt tacggtgaag acataaaata tgaacaagct ctggtagaat acttgatttt
     8341 tctcaatcaa gttgtacaaa aagaagagag tgcgcaagaa cagaatgctg tagttcctaa
     8401 tgagcgtgtg gaacaaatgt tacaatatat ctcaaaaaat ttatctcatc cgctttcttt
     8461 agaggaaatg gaaaagcagt tttttgtcag caaataccat atcacacgag aatttaaaaa
     8521 gtacactgga tttacgtttc atcagtatgt aatgaagaaa aagctgatct atgccaagca
     8581 cttgttgcga gaataccgca gctctagtgc tgtatatagt aaaagtggtt tttcttctta
     8641 tccgcatttt ctgaaagcat tcaagagaga gttcggcata acgcctaaag aatttttgaa
     8701 acgtgataca cagaaacagt tggttcatta tgaacatttt gaagatgaac aatgagaatg
     8761 atcactaaaa aataaccgca gtagctgctg ttgtttacat agaagcagaa actgcggtta
     8821 ttttattttt atctatatat ttcaaaatat tttgaaaaaa tgacgtacat attatatgaa
     8881 tcagctgaat ttacgaaagt ctctcggcga tagtcctgtt tttgctttga acaggcggct
     8941 aaagtaagca aaattctcaa tccctatttc ttcggaaatc attcgaaccg taaattctgt
     9001 ataccgaaga aggtctttag cgtaagaaag ttttttgctg atcaaataat cgattggtgg
     9061 agaaccgata aatttggaaa aatctttgct cagctgatat ttattcaata agaattgttg
     9121 ttctaattga tcaagtgtaa tcgtttcttt gaaatgttcg tctaagtatt gcttcagttt
     9181 gtaaacgtag gcgggaatat cctcttcttc gaaatctagc tgatactttt gcaaaatcag
     9241 ttcattcagc aactcgtgga tagctacaga ggactggaaa tcggtacggg cattcgtgcc
     9301 ttgttgcatg tggatcaatt ctgtgatcaa atggtggatc ttgttttctg atggatctcc
     9361 tgttgaatgg aaggtgttat ggccgtcttt tgtgaactcc tgataaaatg attggacgtt
     9421 ctgaccattc aaatggatcc aatccatttc ccatggttct tcgctgatcg tttgataacg
     9481 ctgatattct tggcagtcaa taaaaaataa gtctccagct tttagctgtt gctgattatc
     9541 attatatgta agcgaacctt tgccagacaa agtaaacttt atcaagtaag aaggtaaatg
     9601 tgctcgttca gtatagtaag ggattggtgc tttgaagtaa ccgatttctt ggatataaaa
     9661 aaataaattc ctgcttcttt gtgtaggcgt caagatcact ctttttgagt cttccgacca
     9721 tgagtggttg gctttttccc agtaatccat gtaaacactc ccaacttgtc aagataatgc
     9781 aattatatac caaaatagtg tgaagcaaaa tataagattc tgtattatga tagcattgtc
     9841 aataaatgat aacgctttat taataaagtg ctaatcaaca ataaagtaaa agaggtggag
     9901 aaatgaatcc aacatttgtt gccatcgata tcggtgcatc aagcggacga ctaatgaaaa
     9961 gtacgaggga tgcaaaaggt tctttgatct tggaagaagt acaccggttc aaaaacggct
    10021 ttcggaacaa agacggtcac gatcgctgga agatcgatta tcttatcaaa gagattttga
    10081 ttggatttga aaaattaaag cagacaggga ttgatcgcct ttttgcgggg atcgatacat
    10141 gggcagtcga ttactgtctg attgatgaac aaggggatcg gataaatgat ccgattgctt
    10201 atcgggacaa gcgtaccaat ggagcagtag aacagttttc tgaaatctat tctctagata
    10261 agttgtatga gcagacaggg attcagatcc agcctttcaa taccttgttc caattgtttg
    10321 ctgaagaaaa agaaaatttg gcaaaagcac ataaattgtt actgatacct gattatttag
    10381 ggtatgtctt tactggtaac atggtgacgg agaaaactaa tgcatctact atgcagctgc
    10441 tgaatgcagg gacaaaagaa tgggaaatag aacttcttga aaaaatcggt gtctcgaagg
    10501 aattatttcc accattgaca gagccgggaa cgatactagg agatctgcaa aaagaacaat
    10561 tttcaaaata tgatttgcca gatgtgacct ttatcacagt agcaagtcat gatacggcgt
    10621 cagctgtatt aggaacgcca ggtcaaggag aacgttgggg ttatatcagc agtgggacat
    10681 ggtcgctttt agggattgaa acaaagatcg ccaatgtttc tcgcaatgct ttttctgaaa
    10741 attatacgaa tgaatggggt gcccataaca cggtgcgttt tttgaaaaac atcatgggga
    10801 tgtggttgat ccaggaagtt gcccgaatgc aagagtacga gtacagttat gcggaattgg
    10861 cagagcttgc tgcaaaagaa aaaccgctgc agcaagtgat ggaaatcaat gaccctcgat
    10921 ttttgaatcc cccaaatatg atagaagaga ttcagcaata ctgttttgaa acagagcagc
    10981 cagtccctgt ctcatcggga gctatcgctc gttgtgtgta tgataatctt gctctttgtt
    11041 acgcacaaga gctgatcaaa gtagaacact taactggaac gaaattggaa aagctgcaca
    11101 tcgtaggggg cggatcgaat aatcgtctac tgaatcaatt gacagcagat tatgccggaa
    11161 tcattgtaga agcaggacct agtgaagcca cagcaatcgg gaatgttgcc atgcaaatgc
    11221 tcagtacagg tcagttttcg aatatagaac aagcaagaaa tgtaatcaaa cagtcgtttc
    11281 catgtgaaac tttttaccca gatgaaaata agcaaaacac gatggaatgg tacgtgaata
    11341 aaatcaaagg agtaatcgta tgaacacgat tgaaagaaat tacgaacaag caaaagaaaa
    11401 atatgcaact atcggtgtag atacagatgc tgtactagaa aagatacagg acatcaaaat
    11461 ttctatgcat tgctggcaag gagatgatgt caaaggtttc ttgacaccgg atggcgaatt
    11521 gaccggtgga atcatggcga cagggaattt tccaggagcc gcacgaacac ctgaagagct
    11581 tagacaagat ctggagaagg cttattcgtt gataccgggc aaacacaaat tgaaccttca
    11641 tgctatctat ttggatacag aagaatctgt cgatctaaat gaaattgagc cgaaacattt
    11701 tgaaaaatgg gtagagtggg caaaaaaaga agaaatcggt cttgacttca atccgacttt
    11761 cttttcgcat ccaatgatga aagacggctt tacattggct catccagata aagaagttcg
    11821 tgaatactgg atcgaacacg ggaaaagaag ccgcaaaatc gcggagtact ttgggaaaga
    11881 aactgggcaa acaagtatca ataacttttg ggtacctgat gggatgaaag ataatccgat
    11941 tgatcgtttt accccacggg aacgtttaat ggaatcactc gatgaaattt ttacagaaga
    12001 gttagatgaa cggtatgtac aagatgcagt agaaagcaaa ctatttggtt taggcgctga
    12061 agcttataca gtcggttccc atgaatttta tatgggttac gggttgactc gtgacaaact
    12121 tgtttgtcta gatgctggac attttcatcc aacagaagtg atttccaata aattatcttc
    12181 attagcttta ttcagcaaag gaatcatgtt gcatgtttcc cgtccagtgc gttgggattc
    12241 agaccatgtg gttttgatgg atgatgaatt gcaggaaatc gctaaagaat tagtccgtaa
    12301 cgaattgttg gaaaaaacaa atatcggtct ggatttcttt gatgcaacga tcaaccgtat
    12361 cgctgcttgg gtcatcggta cgagaaatac acaaaaagca ctgctgaaag ctatgctgga
    12421 accagttgag cgattgaaag aaatggagct ggcttttgac tttacttcaa gaatggctta
    12481 tacggaagaa ttgaaagatt tcccgtatgc cgatgtatgg aattatttct gttacaaaaa
    12541 tcaagttcca gttggtttgg attggctgga agaagtccag gaatatcaaa aagacgtgtt
    12601 ggaattaaga aaataggagt gtggctatat tgaaaaaaat agatatttta caagcgcctt
    12661 atgtgaaaga aatgatcgaa acgacgtcga atctttatcg cttgggatgg gatgaaagaa
    12721 atggcgggaa tatttcttat ttactaaaag aagaagagat cacaccgttc ttggatcctg
    12781 aaaaagtgat ccgccgcatc tcgatgattt ttgatgccag ccgtttagca ggatgctatt
    12841 ttattgtaac tggttcagga agatatttta aaaatgtagc gagcgaacct gccgagaatc
    12901 ttggaatcgt tcgagttgct gaagatggca aaacactgga acttttgtgg gggctggaaa
    12961 acgaatgcct tcctacaagc gagttgccaa gtcatttcat gagtcatatc gcccgtttag
    13021 aagtagaccc agataatcgg atcgttatgc attgccatgc tagtcatcta ttagcgatga
    13081 gttttacgca tgaattagac gaacgttcct ttagtcgtac gctatggcaa atgtgtacgg
    13141 aatgcttagt tgtttttcca gaaggaattt ccatcattcc gtggatggtt ccgggaacaa
    13201 atgagattgg ggaagcaaca gctgaaaaaa tgaaagaaac tcgattggtt ttatggccac
    13261 aacatggaat ctacgggtca ggaaaagaca tggatgaagt ctttggtttg atcgagacag
    13321 cagaaaaggc agcagaagtc tatacgtacg tcaaagcgca aggaccaatc ttgcaaacaa
    13381 tcacggacga aaacctgtgg catttagcag atgcgtttgg tgtcactcca aaagcaggtt
    13441 atttggagga agttcatacg aaagcaggtg tctgacatgg tgaaaaaagc tttttgcatg
    13501 aaagtttacc cagaaaaaca agaagaatac aaaaaaagac atgatgaact ttggccagaa
    13561 atgcgtacag cacttcgtga acacggggtc atcgactatt cgatctatct tgatccgagt
    13621 acaagtttgt tatttggtta cttagtgatc gaagatgaaa agttatggga aaaaatggca
    13681 aatacatcta tcaatcaaaa atggtggaag tacatggaag atatcatgga gacaaatcca
    13741 gatgcctctc cagtcagttg ggaattgatg ccagtctttg aactgtagca acaagcaact
    13801 gggcaaccgg ttgcttgggg ttctttcgtt tttattgtga aaaataatac aaattaggag
    13861 gaatagaaaa tgagtaatcg aatgatttta aatgaaacat cgtattttgg aaaaggagcg
    13921 attgagtcga tcgtccctga atttcagcag cgaggattcg gcaaagcagc tgtcattact
    13981 gacaaaggat tgatcgagca cggtatagca acaaaagtaa cagaacttct agataaagca
    14041 gcaatcgctt atgctttata cgacgggatc gtacctaatc caacgatcca aaatgtaaaa
    14101 gatggagtgg cttttgttaa agaggcagag gcagactgtt tgatcgcaat cggtggaggt
    14161 tcgccaatcg atacggcaaa agccattggg attattttga cgaatccaga gttttcagac
    14221 gtcattagtt tagaaggtgt tgctgatacg aaaaatccat gtttacctat attagctata
    14281 ccaacgactt ctggaacagc agcagaagta acgatcaact acgtcatcac agatgaagta
    14341 aatcaccgaa aatttgtttg tgttgatcct catgatatcc caatcgtcgc gttcatcgac
    14401 agcgatatga tgatgggaat gccgaaaaaa ttggctgcat caacaggtat ggatgctatg
    14461 acgcatgcta ttgaagggtt tatcacaaaa ggcgcttggg aaatgactga tatgctgcat
    14521 ttaaaagcaa tcgaaatcat cggccattct ttggaagctt ctgtagatgg cgatcaaaat
    14581 gggcgagaaa aaatggcatt aggacagtat attgcgggaa tgggcttctc aaatgtcggt
    14641 ttaggactgg tacacgggat ggctcacccg ttaagtgcat ggtacaacat tcctcatggc
    14701 gtagcgtgtg cagcactttt gccaactgtc atgaaatata acaaagaata tactggagaa
    14761 aagtaccgtg agatcgctct tgttctaggg attaaaggag cagctgaaat gtctttagaa
    14821 gatgtaagag aagcggcttg tggtgagatc gatcggttga gcaaagcggt tggaatccca
    14881 gaaaccattt ctgaattagg tgtaaaagaa gcagatattc cagcaattgc agaagatgct
    14941 ttgcgagatg tatgcacacc aggaaatcca agagaaacga cagtagaaga aatcattgct
    15001 ttatatcaaa gtttgatgta aaaacgaagc ggatcttcaa cattaaattg cctgtaaaga
    15061 gaaatggtag tttatatctg gatatctctt ttaaattgat ggtatttccg ctataatttt
    15121 tcttataaag aaataggaga atattatatg gaaaataaaa agccttatgg aactgtattg
    15181 atcaaagcct ctaaaatact agactattta gcggaaaacc aagatgtctc tttgcagacg
    15241 atcgcaaagg gtgtgggaat gacctcttct acagtcttga aaatactaga tactttgttg
    15301 atgatcggct acgtaaataa aaattcagag aaaaactatc gtttaggtgc aaagcttatt
    15361 cgctacgcga ataaaaacat tgaacaaatc gatttggtag agttgacttt accgttttta
    15421 gaacagctgc agcaaaagat cgatgagact atccacttag gggtattgga caacaatgag
    15481 atcttgtacg tgaacaaact agatccaaaa catcaaacga tccgcatgtc ttcaaaaatc
    15541 ggaatcaccc gaccattata caattctgcg atgggcaaag ccgtattagc agaattcagt
    15601 gaagagcagt acactgatta tcttgaaaag catccgttga tcccttacac agaatatacg
    15661 atcaccaatg cattacggtt gaaaaaggaa atcaaaacgg taaaagaaac ccacatcgca
    15721 tttgacgatg aagaaatcga acgtgatatt ttttgtatag gagcctcaat cgtgaaaaac
    15781 gaacaaatca ttggtgcatt tagtgtaagt atgccgaaat atcgtttaac agatgagaca
    15841 aaagaacaaa taatcagtgc cctcaagcaa acaaaaagcg aaatcgagca aagaattcaa
    15901 aaataatgaa aaaagcgttt gcaatttttc ttttctgccg tataatatgg gtatagattt
    15961 ccaagttata aaataaaatt tctaaatagg aaataggtgg agaagatgaa aaaaattgat
    16021 attttatctc gattgaaaaa cgccggagtg attgcggtgg tacgcggaaa aagcaaagaa
    16081 gaagctttga atgcttgcca cgcaatcatc aaaggtggtc taactggtat tgagttgact
    16141 tttacagtcc ctcaagcaga tcaagtaatc aaagaacttt tgtcattcta caaagaccaa
    16201 ccagaaatag tgattggcgc aggcacagta ttagacgctg tcacagctcg cttggctatc
    16261 ttagctggcg cagagtatat tgttagccct tcctttgatc aagaaacggc agagatgtgc
    16321 aatctgtatc aaattcctta cttaccagga tgtatgacta ttaccgagat caaaacagcg
    16381 ctgaaaagcg gggtagatat cgtcaaacta tttcctggaa gcgcttatgg accaagtatc
    16441 atttctgcct tcaaggcgcc gatgcctcaa gtcaatatca tgccaactgg cggtgtgagt
    16501 ctggacaata tgaaagagtg gttcgatgct ggtgtcgtaa cagtcggagt aggtgggaac
    16561 cttttagcac ccgcagctac aggagatttt gacaaagtaa cggaagtcgc gcaacagtat
    16621 gcagcaaaaa tgaaagaaat caagaggtag ctgcttatgg gaaaagtagt gacactcgga
    16681 gaaatcatgt tgcgattgtc cacagagcag ggaacccgta tcgattcaag tgaacgattc
    16741 gctgcccatt atggcggagg ggaagcaaac gtagcaattt ctttagctaa ttacggacat
    16801 gaagcgattt ttgctacaaa agtacctgat aacgcattgg gaaaagcagt caaaaagcac
    16861 ttgcaatgtt atggagtaga tacgaaacag gtattatctg gtggatcaag actaggtact
    16921 tattatatgg aaactggtag tggcgaacgc gccgcctcag tgatctatga tcgtgccgga
    16981 tcaagttttg ctgaaatgga tcatttggaa tggtcgctag aggagttatt ccaaggaata
    17041 gatatatttc atgtttcagg aatcacccca gctttgtcta aaaaatggca aaaaatgacg
    17101 aaacaggtga tagaagctgc aaaaaaagct ggctgcctga tcagcttcga tatcaactat
    17161 cgtggaaaat tatggacaca gcaagaagcc agcgaagtaa tccatcagct tttaccttta
    17221 gtagatatct gctcagctgg aaaaatggat gctctttatt tgttaggtat cgagaaagcg
    17281 ccagaagcag aagaacaacc tctaatttat tattatcaaa aaatgcagga aaagttcccg
    17341 aatattcagg tgttttattc gaccaagcgt accgttcatt cagcaagtga aaatgaattg
    17401 aaggggacat tgtggatgaa ccatgaatat gtggaatccc aagtccatag aatcacccca
    17461 atcgtggatc gagtaggtgg cggagatgct tttgcaggtg gcgtgctcca tgggatttta
    17521 tccaatatgt ctccgcaaga aatcattgat tttgctacgg cagcttctgc cttgaagcat
    17581 acgatccatg gggactgcaa ccaatttagt caagaagaag tcgcacaatt tttagcatgc
    17641 ggctctggaa aaatcattcg atagaaggag tagaagaaaa tgcaagaaat ggaaacacgt
    17701 tatacacaca gtccagaaga tatccgtcat tattcaacgg aacaattgag aaaagaattt
    17761 ttagtagaaa aggtatttga accagggaaa atcagtttga cttatacgca caacgatcga
    17821 atgatctttg gtggagtgac acctactgaa aaagaattag aaatcacatt agacaaagaa
    17881 ctaggtgttg attacttctt agagcgtcgt gaactaggtg tgatcaatat cggtggacca
    17941 ggattcatcg agatcgatgg gcaaaaagaa gaaatgaaaa aacaagacgg atactatatc
    18001 ggaaaagaaa cacgacatgt tgtattttct tctcaagatg ctgaaaaccc agctaaattc
    18061 tatattagtt ctgttccagc tcatcataaa tatccaaatg taaaaatcag tatcgaccaa
    18121 gtaaaaccaa tggagactgg tgaaggcctg acactgaatg aacgtaaaat ctaccaatac
    18181 atccatccaa atgtatgcga aagctgtcaa ttacaaatgg gttatacgat tttagaacca
    18241 ggaagcgctt ggaatacgat gccttgtcat acacatgaac gtcgtatgga agcttatgtc
    18301 tactttgatt atgccaatga agatacacgt gtgttccaca tgatgggcaa accagatgaa
    18361 acaaaacatt tagttgtaga caatgaacaa gccattattt cgccaagctg gtcgatccat
    18421 tcaggtgtag gtacaagcaa ttactcattc atctgggcaa tgtgcggaga aaatatcacc
    18481 tatacagaca tggatatggt gcaaatggat caattaaaat aagcataagg gagagaaaat
    18541 gatggaattt agtatggata tgtttcgttt agatggaaaa gtagcattag tcaccggtgc
    18601 tgtctatggt attggatttg agattgcacg ctctttagct acagcaggag caaaaatcgt
    18661 atttaataac ttgaatcaag aatcagttga tgaaggtata gcgcattata aagaagcagg
    18721 catcgatgca aaaggttatg tctgcgatgt gaccgatgaa gaagcggttc aagcgatggt
    18781 tcaacagatc aaagaagatg taggttcagt tgatattcta gtcaacaatg cagggatcat
    18841 caaacggaca ccgatgatcg aaatggatgc ggcagacttc cgccaagtaa tcgacgtgga
    18901 tttaaatgcc ccattcatcg tttcaaaagc agttattccg gatatgatcg aaaaaggcgg
    18961 cggtaaaatc atcaatatct gctccatgat gagtgaatta ggacgcgaaa cggtcagtgc
    19021 ttatgctgcg gcaaaaggcg gattaaaaat gctgacgaaa aacatcgctt ctgaatacgg
    19081 tcaatacaat atccaatgta atggaattgg accaggttat attgctacac cgcaaactgc
    19141 tccattacgc gaaacacaag aaaacggtga acgtcatcca ttcgatcaat ttatcgttgg
    19201 acgtacgcca gcagcacgct ggggcgagcc aatcgatttg gctggaccat ctgtcttctt
    19261 ggcttctagc gcatctgatt tcgttaacgg ccatgtcttg tatgtggacg gaggcatact
    19321 tgcctatatc ggaaaacaac cttaaaagag ctttagtaca ctgtttagtg gagaggttgt
    19381 gaaatcagcg gattgaacga ataccgaagg acagctgatt aaacatcatt tattcgaaaa
    19441 aagaggctgg gacagaagca tttaactcca agaaataaga aggaattcac gaaaattgct
    19501 tttcaaattt ttgtgaattt cagcttattt ccgaaggagt tgcttttact tccgccgttt
    19561 gtacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tcttttctgt
    19621 gcgtcttcca tacttcacta agtcgatccc tcttgcttcg caccaatcct tcactggtcc
    19681 ggaaaagatc agagattgtt ggtgtagggc cgcggtattc gttgcgccga ccatagtata
    19741 aagcagacga agctcttctt gccattgctt catcagtata atagtttctt ctacgccgtg
    19801 attcatcaga tgcgtcagta cggttcctga agtacccacg gcatttgcgc cgagacaaag
    19861 ggctttaaat atgtcatagg cattgcggat acctccggag gctaggattt ccatagaagt
    19921 gtctgcttca tttgcttcaa gcaatgaaga gactgtcgat tgcccccagt cagctaaata
    19981 actcagttcc cgcttggagc gacgggcgtt ttcgatctgg gtgaagcttg ttccgcttcg
    20041 accgctgata tcaactgtat gaactcctaa agcagctaga tcattgagcg tttctcttgt
    20101 catgccaaaa ccgacttctt ttacgatgag ggggacgtca atggctgttt gagtttcttg
    20161 aataagtact ttccaattgg taaaatcacg gtctccctca ggcataacca actcttgagg
    20221 tgcattcaaa tgaatctgta aggcatctgc gtgaaacaat cggatagctt ctttggctct
    20281 ttcgacagaa gtccctgcac cgatgttggc aatgattttc ccgtgaggat attcttgtcg
    20341 cataatcgta taagtatcgg ctaaagaagg gactttcaag gcagcgctga ctgaaccagt
    20401 ggcaatcatc aaatctgctt ctcttgcgac gattgcaaga tcttggttga ttttttttgt
    20461 tttttcgctt ccgccagtca tcgcattgat ataaaatggg ctgcttagtg taaacccaac
    20521 tgcctgtgta cttagatcga catcagaaac tgctatttgc ggcaaagggt tatggatgat
    20581 ccgtacgaag tcaaattcgt ttttttgttt atcatgaaag gctttagcta gtgaaacatg
    20641 ttcgtctttt cgattcatcg gttttccttt ctttgatcgc tatagacatg taacggcagt
    20701 ggagtgattt ctgctttttc ccatgcactc attaaaggaa gaatgccaga tttctggtca
    20761 acaattacga ttccgcaatc gcccccacct gcaccagaag attttgcggc tccttcatac
    20821 tgttcagcta aattacacaa tttgttcaaa gcaggcgttt cgatgacgac cccagtgatt
    20881 gcagataaat catgcagtaa ttgtcggttt tttcgaatca tctgttggat caacgttaca
    20941 ttattttctt taaacccttt gatcatttcg ttgacacatt ctgtactatt ttttaagaaa
    21001 agctgataag ccaccatttt atcttctctc gaacggtgaa cttgatcgac caaatcagaa
    21061 gtagaggcag ggctacccgt ccaaccaatc aataaacgta aatcttcagg agcaatcaat
    21121 ggctcaatgg atagacctgg ccaatccagg gcaagtaact cactgataga atgctgagtt
    21181 tcttgttctt ggagccaagg atggtcgaag gttgagaaag cgatccagcc accataacag
    21241 ctagcagcga tgtcgccgca ggaaccatta tcttgaactg ctaaattggc tagtgctgca
    21301 atcttgaaaa tctccaactg agataaattc aacgcgtaaa aaacattcaa ggctttgacc
    21361 gttgcaacgg tcacggcacc gctagaaccc aatccatatt tccggccatt tgaactgtct
    21421 aactcacttg ttactttcag atcataaaag gataaaagaa tgtttttttc ttgtgcatac
    21481 ttttcagtca agcgaatagc agcaaggata taatgaaaag gattttcccg aatatctaaa
    21541 actaattctc cgttgcgtct tgtccaacgt acaggcatcc cactatattg tgcagattgg
    21601 atacttccga cttttcgtgc agattctaca gtgactgtca cgaattgatc gactgcagcg
    21661 ataactgctg gatggcctgt ttcaacaact gcatattctc cggcaatata gagtttgcct
    21721 ggtgcagata cttcaatcat aaaaatctcc ctttattcaa taatcgcaat tcctggacca
    21781 gcatgtgcag taattacttg ttctttgcta aataatttgg taaatgtttc ttgtacctca
    21841 gataaatggc tgttttccac taaaactttg acattagggc cagcatccat cgtaaagtag
    21901 catggaatgc cttgctttct caattgtcgc accgcatcca tcgcttttaa gctgtcagga
    21961 gaccagtaag tgaatggagg ctgggcagct aaagtcgttc catgcatttt tagtccgttt
    22021 ctttccatcg tttctccgag aagttggaaa tcttttgttt tgattgcttg tttcaattga
    22081 tatagatccc cttcaacgga atctaaccag ccttgataaa aactggatgt ttcgactgtc
    22141 cgacgcatcc catttctgct ggacacttct tttttctggt cgttgatcaa aacgaaaacc
    22201 attgctaaat cgtcttcgaa agaatcggaa gggactggct tagcgtaaga acttaagtcg
    22261 tcatggcctt tttcccattc gacgaaacca ccgaaaatac ttcggcaagc tgaaccagac
    22321 ccgcgtcgag caaatcttga aagagagaga tcatctaatc caagcttaag agcagtattg
    22381 caagcgcctg ctaaagcagc tagcccgctg gcagaggagg ctaatccagc tgcggtagga
    22441 acgaaattct gactgattac tgaagctttt agtgagaggc cggcttcttt acgaaaaagg
    22501 tcaagaaact gactgacttt ttttgttgct ttttcgtctt gcaaggtgcc atctagataa
    22561 aattcatcta ccatatagct atcagaaaag atgacttctg tctctgtata aaatgcatcc
    22621 aatgttaacg aaaggctgtt gttcattgga aggatgagtt cttcattttt cttaccccaa
    22681 tatttgatta gagcaatgtt ggtatatgcg cgtgctttgc ctttaaacat aggtatgtac
    22741 tcctaatcct tgtatccaag tttcagcagc acccgcatct tcaagtgctt gactgatttc
    22801 ttgggctttt gtttttgttt gtgctaatgc aatcatacag ccaccgcgtc caccgccagt
    22861 taatttggca cccaatgccc cggtatcttt tgctgtttgg atcaatagat taagaaaatc
    22921 attgctgatt gtcagctttt ccagaagtga ttgtgattca tccattgtct gtgccaacgt
    22981 ttctggtgaa ttttcaatga ttgcttgttt tgcttgcttc gtcaagtatc ctaatttttg
    23041 gatcatttgt ccagtttcat gaggctgggt ttcaaagagg tgagcgacat ctttgacggc
    23101 ttctcttgtt tgtcctttga ttcctgtatc agcgacaatc aaaaaagcat cgatgttcaa
    23161 agaaaagtag tcgaaaggat gcccttttgt aaaataaatc ggctgcaagc tgctagtagc
    23221 ggctgcatcg attccactag gattaccatg ggcgattttt tccgaaagct ggacattttc
    23281 taatagtatt tcacgagaca aaggaaatgc taagtaatca taaaaagcac gagtgactgc
    23341 tgtggcgact gcagcgcttg atcccattcc acgttcagca ggaatcgtgc tttcaatggt
    23401 cagttgcaag tttttttgga tgtcatgctg tttttttaag tgaatgaata attttttgat
    23461 attttttaat gcatgaggag cttcggctaa atttcctgaa tagtaggaag aaaccagttg
    23521 atcgtccatt gcatccagct cttcaaggaa tgcggtgact tttgttgcat agaaaggaaa
    23581 ggcaatcgct ggttctccat aaacaaccgc gtgctcgccc atcaatatga tctttccgct
    23641 tgattctcct tggccatagt ttgccataat ctaactcctt ctcacgtaaa agtgattttt
    23701 aagcaaaaaa taaagcgccc aaaacgagtt gcgctgcttc acgaattttt ttgtccacta
    23761 agggtctcaa aaaacattcc tcctttattt tactaaaacc atctcagaag taaaactaag
    23821 aatacgtttt ctaaagaaaa aacacttttt tcttaagaat tttttgactt tttttcttct
    23881 ttgactagaa aaaaatacaa ttgacactac tagatattgg gggtatattt aaaaaggttg
    23941 gagttaaaca caatatttag tgatgaagtg agctggaggt aaatgagcgt gatcgtttta
    24001 gcaggtacaa ttggtgccgg aaaatcaagt ttaacagaaa tgatggctga acactttgat
    24061 agtcaggcat tttatgaatc gattgatgat aatgaagtct tgcccttatt ttatgcaaac
    24121 cctgaacagt atgctttttt gctgcaaatt tattttttga ataaacgttt tgcaagcatc
    24181 aaacaagcaa tgaaagatga caataatgta ttggaccgtt cgatttatga agattcactg
    24241 cttttccatc taaatgcaga tctaggaaga gcaacagaaa cagaagttcg tgtgtatgat
    24301 gaactgctgg aaaacatgat ggaagaactt ccctatgctg cccataaaaa acatccggat
    24361 ttattagttc atattcgtgt atcttttgat acgatgttgg agcgtatcga aaaacgtggt
    24421 cgttcgtatg aacaattgtc ctttgatcca agtctatacg attattacaa agaattgaac
    24481 agacgttacg atcaatggta tgaagaatac aaagaaagtc caaaaatcca aatcgatgga
    24541 gaccgctata attttgtgga agatcctcaa gcaaaagaag aagtcttgaa aatgatcgaa
    24601 gaaaaattag cagagattcg ccaaaccaaa gtagccattt gatttacagt tgctcaaata
    24661 aagggtaaac ctttatttga gcgacttttt tctttatact ggaaacaaat gtcaggagga
    24721 tagagtatgc gggaaaaaca ctattttaat caagaccgta atttttggat caatgtacag
    24781 caaaacgatg tgctagctgt taaaaactta aaagaagaat atggcattag tgacgagatg
    24841 ctgacttatt ctctagataa aaatgaacgg gcgcgcgtag agtacgattc ttttgatgaa
    24901 gcgttactgc ttgtatcaaa tgttccgcac cagcaaaaag tcgataatca ttatgaaact
    24961 agtcctatcg cttttatttt aaaagaagac ggtttgttta catttaccac gccgaatacc
    25021 gagtacgtga ttcgactaat tcgatcgctt ttggaacgaa tgccagacat gagtgtatac
    25081 agtttattgt ttcgtacatt gtttttgatt tctgattcct tttttccttt aatcgaggaa
    25141 gtgaatagtg aacgtcagcg gcttaatctg aaattaagag aaaaaacaac gaacaagaat
    25201 ctgctgcaac tttcagattt agagatcggt ttggtttact tagttacagg aacaaaacaa
    25261 aatgtagtac ttttagaaca aatcaaggca ttggctattt atcgcaagtt gtcagaaaag
    25321 gaaaaagaag agttggacga tgcgctgatc gaagcacgac aagcagttga aatgacgaac
    25381 ttagcttcgc agattttaga ccagctctct ggcacttata ataatttgct gaataacaac
    25441 ttgaatgaca cgatgaaatt tttgacggtc tggtcgctga tcttgaccgt tccaactatt
    25501 gtgacaggat tcttcggaat gaatctccag ttacccttta cccattccgt tttcggatgg
    25561 ggaatcgcct tgatcatcag tttggtctta tctatttgga tgcttattgc actgtggcgg
    25621 cggattcgtt gatctagtct ttgatctgca aaccaagggc ttgggcgatc acaagtgctt
    25681 gctcttggtt gacgattagt cccgccaaag aatcttgaga taaagcgatt gctgtgaaga
    25741 aattagtcgt aaagtctaat cccttcagag aagttcgaaa ccagttcgaa ccggtcaact
    25801 gattgtcaga caaaaaaagg tttttccaag tgacctcata aaattcgctg tcttctaatt
    25861 gacaatgatc gaaggagaca gctttcaagt ttgtactact aaaggaagcc atcgaagcaa
    25921 tacaatcagt gaaagtacag tctcgaagat aactttcggc aaaattcgta cctgttaatt
    25981 tacattgatg aaacacgact tggtggaaac tgccaccaat ccactccaga ttggaaaaat
    26041 cacatttttc aaaaatgaca ttactgcatt caaaatgacg taaaactgtt ttttgcatcg
    26101 taagtttttg gatatgactc tctcttaata ccatattttt ggcatcgaga tagctttggt
    26161 cacccccgtt gatcagaaga ccagagaaga tctcctcatc ttctaaataa aactctccct
    26221 ttgtaagaag aggaagagaa ggtggaatag gttcgatttt tttcatgtgg gttcacctcg
    26281 tttttcttta gtatagcaaa gaagcgggaa tcactgctag aaagtctgct aatctaatca
    26341 ttttcagaaa aggaagacaa actgtatgaa aaacattgac aaaaagatta gtttttgcta
    26401 tactatgatg gttgagaaat aaaagcagaa gcacccgctt ctcgccttag ttgataatgt
    26461 cactgggcta gatagttttc actggttcta ttaggtgaag tacgcgcggg ttctgtaaga
    26521 gaactcgttt ttttattgcc gtttattcgg taagcttttc tttctctcat aatatttgga
    26581 ggtgaatgac catagcaaag gatatgatgg ttaacgacgg cattcgtgca cgagagttac
    26641 gattgatcgg ttcagatggt gagcaattag gagttaagac aaaagcagaa gcattgaata
    26701 ttgcagaaca agcaaacttg gatcttgtgt tagttgcccc tggtgcgaaa ccaccagttg
    26761 cgcgaatcat ggactacggt aaataccgtt tcgaacaaca aaagaaagac cgcgaagcgc
    26821 gtaaaaaaca aaaagtgatc aatgtaaaag aagttcgctt aagtcctacg atcgacttga
    26881 atgacttcaa tacaaaactt cgtaatgcac gtaagttctt ggaaaagggc gataaagtga
    26941 aagcttctat ccgtttcaaa ggccgtgcca ttacccataa agaaattggt caaaaagtct
    27001 tgaatcgctt agctgaagaa actgcggata ttgcaacagt ggaacaaaaa gcgaaaatgg
    27061 acggacgcag catgttcttg acgctggcac cgaaaaacga cagtaagtaa tttgaatgac
    27121 taactggctg ttggacagcc gatagtcgga taacgaaaga ttttgaggag gaaatatagt
    27181 catgccaaaa caaaaaacac accgcggatc agcaaaacgt ttcaaacgta ctggtaaagg
    27241 cggattgaaa cgcttccgcg cgtttacaag tcaccgtttc cacggcaaaa caaaaaaaca
    27301 acgccgtcaa ttgcgtaaag caagcatggt ttctagtggc gacttcaaac gtattcgcca
    27361 acaactagca aaaatgaaat aagaagtaca aataacagaa tctgaagatt tatttttatt
    27421 aggaggaatt atacatggca cgtgttaaag gtggaacagt aactcgtaaa cgtcgtaaaa
    27481 gagtgctaaa attagcaaaa ggttactacg gttcaaaaca cactttattt aaaacagcaa
    27541 aagaacaagt aatgaattca tacaactacg catatcgcga tcgtcgtcaa aagaaacgcg
    27601 acttccgtaa attgtggatt gctcgtatca acgcagcagc tcgtatgaat ggcttgagct
    27661 actcaaaatt aatgcacggt ttgaaagttg ctgaaatcga catcaaccgt aaaatgttag
    27721 ctgacttggc tgttaacgat gcagcagcat ttactgcatt ggctgaccaa gcaaaagaag
    27781 ctttggctaa ataagctaaa caattataaa gaggaaagaa gataacgaaa agttatgttc
    27841 tttcctcttt ttttatttac aatcctccat caaatgaaac aggacaataa cgtataagtc
    27901 taaaatagca taattaaggt tttttaattg aaaactgaac atttatggtc accctttaat
    27961 gaaagcgcta actacataag ttagaatgag tttgactgat atattagttt gcttatcaga
    28021 aaaacaaaat ggacggagga ataacatgag tgatattatt attggtattt tgttagttat
    28081 ctcattcttt ttcatggtct ggtactgtgt aaaaggctac aacttgatgg tcggctttgc
    28141 catcatggct actgtatgga tgggattagc gctagtcgga aacacgtttt caccaaaccc
    28201 ggcgatggaa gggcaaggag taattgatgt gttgacgcat atctatacga cggggccagc
    28261 tgagtacgcc aaatcgatct tggtaaatgt cttctttggt gcctttttcg ggcgtgttct
    28321 agtagatagc ggaatcgcag ctaccctgat tcgaaaggta gttgaacttg gaggggacaa
    28381 gccaagaatc acgatgagct tactatgtat tgtaaccgct gttatattca tgtcgatgac
    28441 aggaatcgga ccagtgattt ctattgcagt tattgtgttg ccaatcttga tgtcattagg
    28501 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
    28561 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
    28621 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
    28681 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
    28741 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
    28801 agttttaggg gtcgttcttt tagatcttcc aatcatctta ggttttattc tagcaggtat
    28861 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
    28921 tgcgaaatta tttacagatg gcgcagtaga tgttgcgcca atggttggtt tcttgatgac
    28981 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
    29041 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
    29101 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
    29161 attagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caatcacaac
    29221 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
    29281 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggagc
    29341 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
    29401 aaaacgatta gatgaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
    29461 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
    29521 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagccgcagg tgtcgttcag
    29581 tcttttcaaa attgtttgct ggaaaataat atcagtccag aagatgttgt gtttgttgca
    29641 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
    29701 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
    29761 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
    29821 cacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
    29881 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
    29941 gaagcagcat cggtaaaatc gatccctaca acgatggctt ctgacatcac aaaattatat
    30001 ggattgacaa gaagaactcg gacagcagca atcaatgcat ctattttacc aaaaatgcta
    30061 gatacggcaa cctctacaga agattccgta cgagaagcag gggtcaatgt ttctttgatg
    30121 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
    30181 accatgcttt ccggtccagc agcatcagtc atgggctctt tgatgtactt gagagcgtct
    30241 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
    30301 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
    30361 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
    30421 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
    30481 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
    30541 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
    30601 aatgtccttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
    30661 aaagccatcc aagcattagc tgattattgt catacgacgg ttgaagacat cgcagaacag
    30721 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
    30781 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
    30841 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
    30901 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
    30961 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
    31021 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacagac ttcaaaagta
    31081 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
    31141 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
    31201 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
    31261 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
    31321 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
    31381 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
    31441 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
    31501 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
    31561 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
    31621 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
    31681 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
    31741 cgatgtagga gaaatttgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
    31801 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggattc gattgatgac
    31861 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
    31921 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
    31981 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
    32041 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
    32101 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
    32161 gatcttagaa gaaataaaaa aaatagaaaa aagatgtacg gagtatgatt tagattacaa
    32221 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acgatgttct
    32281 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
    32341 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaacggtgtt
    32401 caaaagcttg catctgcggt aataagtcca gccaagttaa aaacataaga agctattttt
    32461 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
    32521 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
    32581 ttgccgtttc tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
    32641 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
    32701 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
    32761 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
    32821 aatcgtcaaa cgtaaccatc tgaaacgcgt actataactt tctagccaat cccaaatttc
    32881 tcgttttcca gcaatttcat aacaagttcg gtgaaaatca gtatccaaat caaaaaaagc
    32941 ttttttattt tgagcgatta ctgcctgatc ggtttgttct agtatttttt ctagtttagc
    33001 ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg atcggttttt ccaacttttc
    33061 ccgcgtataa cgggctaatg aagcagatcg gatatcgatt tttgaaacat aagtgccgga
    33121 ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg atcagtgctt ctcgaatcgg
    33181 tgtacgcccg atagctagtt gttcttctaa cactttatcg gagattttac ttccaggtgc
    33241 taactcggaa taaatgattt gtttacgtat agattcataa gcttgctgtt gcaaattatt
    33301 gataaccatt taaccttcct ttctatgcag atataatcta ttataacata aatcgcttac
    33361 aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc ctgataggca tgcctattag
    33421 agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc tcttggatcg gtttgacacg
    33481 accgccgatt cttttagcaa aagcttcgga ttcttttttg tctttgaaaa tgagcagatt
    33541 gtctaagttt ttttcccgag tgcgacagcc agttttatcg atgtaaccgt gcattacttt
    33601 aacgacatac atggggatca cctgcttttt tcttttcatt atacgaacta ctcgacacaa
    33661 tacaagaaaa atgcttagac ggtctttatt aagaatttac atcatccaaa gaagcgtacg
    33721 cttggaataa ctgatctaac tccggattga ctttgctcag cttcactaat cgttgcgaat
    33781 cagaaagtaa aaaacagtgt ttactactgc cagtagtttt taattttttg tcttcagttc
    33841 ccacaatttc gtagctaaaa tccaaccgtg tccccgtata cttttggata gttggatgga
    33901 tgctcacttt ttctccgtaa tggatcattt ctttatagct gcaatttact tccagaacgg
    33961 ggatgatgat cccagattct tcgatttttt tatagggaaa ccctagatgt accaataagg
    34021 ctacccgagc ctcttcaaac cagcggatat aattgctgtg atgaataatc cccatttgat
    34081 ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa tttctccatg actcatgcct
    34141 cgttttccat tgatttttta caaaaataat tcatccattt cggaagtgct tcgctgattt
    34201 gtgtcggcaa aacaatatat tgttcttttg ccagctggtc tccaatgaaa ctatgaagat
    34261 aaatagctgc tgcaatcgtt tggtcatttt gaggaaactg ggctaaaaat cctgtgatca
    34321 tcccagctaa agtgtcaccc atgccgcccg ttgccatagc agcagaacct aaaggattgg
    34381 tgtagaagtg ctggtgacca taaatttttg tctcatgact tttcaaaacg agcacagcac
    34441 cgatttcctg ttgcttcttt ttgttttctt tatcccgttg ttcttgtatc ggtagagaac
    34501 tcaaccgttg ccactccatt tgatgaggtg taagtacagt ttgttcggga tagggtagca
    34561 aaagatgatt agcagcaagt aaacttaaag cagagccatc aatcaccagc cattgttctt
    34621 ttgattggtt ttctagtact tgtttgaaac gttccaaact cttagaagaa gtacccaatc
    34681 cgggaccaat caagagtaca tctgcttgct ttattacgga aagaaactgg tcagtatccg
    34741 tccaaactac aatcattgct tctggcagat gactgtgaag agcactccga tttttttcat
    34801 cagttactac agtcgtgaga cccgctccgc tgtgtacggc agctaaagca ctcataatga
    34861 ttgctccgcc atattgttca ttgccgccaa tcaaaacgat acgtccaaat gttcctttat
    34921 gtgaaatttt tggacgaggt gtgatgactc ttttcactag ttgttcgttt atcagcatct
    34981 aaaaacctcc ttttactcat tatagcgtat atttttttta gagaaaaaca gataagaaaa
    35041 ggaattgtca gattttttga actatttttt tctgtaattt gtggtatcat gaacacgaag
    35101 acttaaataa gtggaggatt aactttatga caattgattg gcaaaaagaa gtagaagcac
    35161 gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg tgtcaacagc gagcgcgatg
    35221 actcaaaagt aacaccagat gcaccatttg gacctggacc tcgcgacgca ttgaaacata
    35281 tgttagctta tggcgaacgt gatggattta aagtaaaaaa tgtcgataac tatgcagggc
    35341 atattgatct tggcgaagga gacgaaacgc tagggatatt tggacacatg gatgtcgttc
    35401 ctgcaggaga tggctgggac acagatcctt atgaaccagt aatcaaggat ggaaagatct
    35461 ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc agcttactat gcgatgaaga
    35521 tcattaaaga acttgacttg aaactatcaa aaaaagttcg cttcgtagtc ggcagcgatg
    35581 aagaaagcgg ctggggcgat atggcttatt atttcgaaca tgaagaagaa ccagattttg
    35641 gtttctcacc agatgctgaa ttcccaatca tcaatggtga aaaaggaaat gtttctttag
    35701 ctttacgctt caaaggcgac aatgcaggag attatgtctt gaaatctttt gtttccggac
    35761 ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact tcaagtacca agtgcagatg
    35821 cagctatcgc gatggaagaa gcattttacc aattcatcga agcaaatcca gtaagcggaa
    35881 cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt tggtaaaggc gcacatggtg
    35941 caagcccaca atctggtatc aatgcaggtt ctttcttggc attgttctta gataactatg
    36001 aattcctagg atctgctaaa caatttatcc atgtagcagc tgcttatgtc catgaagatt
    36061 tttatggaga aaaactagga gtcgcttatg aagatgaaaa aatgggcaaa ttgacaatga
    36121 atgctggact atttgctttt gaagaaaatg gtacagagga agcgaacttc atcaatatga
    36181 atttccgttt tccaaaaggt gtaacagtag acggattgca aagtgacatc gaacaaacag
    36241 tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt aatggaacca cattacgtac
    36301 caatggatga tccattggtg gctacattat tgcaagtcta tgaagaccac actggagaaa
    36361 aaggctacga acaaatcatc ggtggcggaa cttacggacg tttgttgaaa cgtggcgttg
    36421 cttatggagc gatgttccca ggatatactg atacaatgca tcaagcaaac gaatttatga
    36481 gtcttgatga cttgttcaga gcaacagcaa tctatgcaga tgccatctat cgtttggcga
    36541 aataattaat taaaaagagg atcaagaatt gcaaattatt tttgcagtct tgatcctttt
    36601 tttactttct actgtggtat tcatgataaa catgatatag attcaattca tattctttct
    36661 tagcgttcgt gacgactgta tccaaaatca accctgttac taatgaaaga agtgctaaag
    36721 tcatcaatcc agtacttaaa atcgcagaag ggatgcgtgt aataaagctt gtcagcatat
    36781 attcgcgaat cacaggaatc cccgtgacca atccaaataa aaagaagaag atggtccaaa
    36841 tcccaaagaa aagcaaaggc ttataatctt taaacatttt aataatcatc atgatcactt
    36901 tgaatccgtc agagaatgta ttcaattttg attcgctgcc ttcaggacga tcacgataat
    36961 cgattgggat ttcaacgaac ttgaattttt tatctaaagc atggatcgtc aattccgttt
    37021 cgatttgaaa accagaactc ataataggaa aacttttgac aaagatacga ttgaatcctc
    37081 gatagccggt catgacatca agaatttttg ttttatacat tctagtaatt gtatttctaa
    37141 caaggttatt tccaaaatcg tggaaaggtc tcttattctc atcaaagtag gttccatttg
    37201 ataggcgatc accaatgacc atatctgctt caccagaacg taatttctcg agcaaaccat
    37261 gtacagcttc tgcaggataa gtatcatcgc catctaccat taaataatag tcggcatcga
    37321 tatcaaagaa catttggcgg atcacatttc cttttccttg acgaggttct ttttttacga
    37381 ttgctccacc ttgaacggcc aattcgtatg ttcgatcggt cgaattatta tcatagacat
    37441 aaatatctgc ttctggtaat tctcgtttaa agtctgcaat gactgttgaa attgttgctt
    37501 cctcattgta acaaggaatc agaacagcaa ttttttcttg ttgttccatt tttcactctc
    37561 cttaactata gaaaacaaaa gctaaattaa aaatacctca taatcaaagg aaatgctagt
    37621 ttttcccgct aaatatcatt tatttaaaaa gaaaatactg gaatatatgt ttggaaagct
    37681 ctatctaaaa aaccacccac tacaaaagaa ggacatgata cctctctttt gaatagtggg
    37741 tgcgtattcg ttctctcact tttttgacgt attaatctgt tgcacctttt tttcttgaaa
    37801 tttctaatac ggttcctgta tgagcatctg ctaaaaattc ataataaaca gtttcgccat
    37861 cttctattcg agaaagacct ccccgataag cactcgaccg aacggcgaat ttcctaatag
    37921 gttccttttc aaaagagatc caagaacctt cgattgcacc ttcttttaga aaagatgact
    37981 tgatattttc aagtacttgg tctgctgaca atactttttt gcgatgatac caaatcgttg
    38041 aagctacccc gctcaataat cctagaccga ttccaactgc taacccgcct ttaaaatagc
    38101 ttagttcgct ttcttcatac atgtggacat acctccttct tctcacttgt tactccttgt
    38161 gcttatttta ccacactctc atattttctt tgaaataaat aaccgctacc agaaaaaata
    38221 agctataata aatacgatcg gtctgcagaa aaaatcatat tttatccgca gatcaatata
    38281 agtctgagag gagtttagtc tgaaatttag acgaatagca tatggaagaa aaaacatttc
    38341 aacgaatcaa agagttaaca gaattacaag gaacaagcgg ttttgaacaa gatgttcgcg
    38401 cttatatgga aagtcatatg gaaccgctgg tagatgagtt acagttagat ggtttgggcg
    38461 gcatttttgg tctgcgccat catgaagaag cggatgcacc ccgtgtaatg gtcgcagcac
    38521 atatggatga agtcggattc atgctgacac aaatccaaga aaacgggctt tttaaagtcg
    38581 tacctctagg cggctggaat ccttacgttg tttctgctca gcgttttaca cttaaaacct
    38641 ctactggtaa gaattatcca tgtatttctt cttccgttcc cccacattta ttgcgaggaa
    38701 caagcggaca aaaatcagta gaagtaaccg atattttgtt cgatgctggg tttgaatcaa
    38761 gagaagaggc aatgagttac ggtgtccttc caggagacac aatcgttcct tacgctgaaa
    38821 cgatcaaaac agccaacgga aagaatatta tcagtaaatc atgggacaac cgctatggct
    38881 gtacaatggt attagaagct ttagaagcgt tgcaaaatga aacattaggt catacattga
    38941 ttgcaggcgc gaatgtccag gaagaagtag gcttgcgtgg ttctaaagct tctgtcaata
    39001 aattcaagcc agatctgttc tttgcagtag actgctctgc agcagatgat acagtgacga
    39061 aaaatggcac attcggacat ttaggagaag gtacattgat gcgtatccaa gatccaggtt
    39121 tgatcatgct tcctcgtttg cgcgagtact tgctggatat cgctgaaaca aacaatatcc
    39181 cttatcaata ctttgtttca aaaggcggta cagatgcagg tgctgctcat acacaaaacg
    39241 aaggaatccc aagtacagtg attggcgtag tgggacgtta tatccatacg catcaaacga
    39301 tgttcagtat tcgcgatttt gaagctgcac gagaaatgtt gattcaaaca ctgaaaggtt
    39361 tggataaatc aacagtaaat acgatcgtat acggaaaata atgcgatagt gaaactataa
    39421 aatctggcaa aagattgctt tttgccagat tttttttata aaaagaggat caatgtttgt
    39481 tagttcgaaa gaaaggtgta taatttaatt gaaaaataca taaacagaaa gtagaggaca
    39541 aagaatgatt atcccaaata gtttagaaga attagcaggc tacgtagaaa ctggaaagag
    39601 tgtatttttc tttactgccg gctggtgcgg agactgccgt tttatcaaac cgcaaatgcc
    39661 tgaaattgaa aatgactttt ctgattggca atttattgaa gtagaccgtg acaagtacat
    39721 agatgtagcc gctgaatgga acatttttgg tatcccaagt tttgtcgtga tccaagacgg
    39781 aaaagaatta ggtcgtctag tgaataaaga tcgtaaaaca aaacaggaaa tcgaatcgtt
    39841 cctagaaagt gtaacaaaag gctgacatct gttaggaggg gtcaagatga atcaagaata
    39901 taagaatatt ttagtaggta tcgacggtag tgaacaagca cttgctgctt tcaaaaaagc
    39961 ggtagaagtg gctcgccgta ataatgggaa agtatatgta gcaaacgtaa tcgatcagca
    40021 attttataac tttatgggct atgcacctat tgatcaaaat ctagtcgatc agcaaactga
    40081 aacagccaag gaaatgatcg aagaatgtaa agaatatggg aaatctgttg attacacaaa
    40141 tatagaagga attattgcat atggttctgt tcgtgaatcc atggcagtca aattgccaaa
    40201 aaaatatgag atcgatctaa tcatggttgg gcagtcaggt ctaaatgcag tcgaacgctt
    40261 tatgacagga agtgtagcca gctacgtgat acgtcattca ccttgtgatg tcttgatcgt
    40321 ttctgacgaa gaagaacagc caagtaatta agcaagtgga acttgtacga cctttttaat
    40381 caattaacta aaacagtaaa gggactgtga caaaagtctt gtcacagtcc cttgttacga
    40441 ataaatggtg ttccagaaga ggcttcttcg gaaataaagc atgaaaaaaa tttcatgcag
    40501 tgaatgcaat tttttgttaa gataaatctc agcaaaggat aatggaggaa agaacatgat
    40561 ttttgcttat aacaaagcac acgtcggaga caccttgatg gttattatag ccgacgataa
    40621 aggaacagaa aacactgttg aacgcaaatg gaatgttgct cggatcaaag atgaacaagg
    40681 tcaagttgtt gcttggaact ttttccatat ctccgatcat ctaacaatcg aagggaacgg
    40741 ccaagtaagc ttaacagatg aggaattagc gaaattgaat cgattgatca aagaagcagg
    40801 attcgaagaa cagcttgaag ctgatcatga accgaagatc gttgtcgggt acgtaaaaac
    40861 atgcgtccca catcctgatt ctgaccattt atcgattact gagacagaag tagacaatgg
    40921 tacagtattg caaatcgttt gcggtgcacc caatatcgaa gctggacaaa aagttgtcgt
    40981 tgcaaaacca ggagctatga tgccagatgg aatgatgatc tggccaggaa ctttgcgagg
    41041 ggtagatagc tatggaatga tctgttccgc tcgtgaattg catttgccaa atgctccagc
    41101 aaaaaaagga atcttagtat tgcctgagga tgccgtagtc ggagaagcat ttccaaaata
    41161 aagaggttgt gacaagtctt tgtcacaacc tcttttttat taattttcac tattttgatt
    41221 ttgttttaat gccgattgat cgatcgtcaa ttcaacagta gttgtttttt ctttcgagcc
    41281 ttcatagtaa gtgattttga cagaatctcc cacttttttc ttatacaaag cagattgaag
    41341 ttctacacca gaagaaactt ctttgtcatc gatcttagta atgacgtcgt attgtttcaa
    41401 tccagctttt tcagccggtg tagctgtttg gacatttgtc acgatgacac cgttagttac
    41461 agatgaagga atcttcaaga tttgttcttg ttgctgagta gaaacggcag ataagtcgac
    41521 cattgtgata cctagagctg gacgtgtcac ttttccgtct ttttccagtt gattgatgat
    41581 attcactaca tcgttacttg gaatagcaaa tcccattcct tctacgctta cattagaagt
    41641 agattcagaa gtacttgcga tcttacttga gttgataccg ataacttgtc cttcgatatt
    41701 aactaaagga ccaccagaat ttccagggtt gattgctgca tctgtttgga tcgcattgat
    41761 attgactgtt tcgttcgatt cattcgtact tgtcacttgt cggttcaagg aagaaatgat
    41821 tcctgaagtg acagaatttg catattcaga tcctagtggg gaaccaatcg caatcgcagg
    41881 ctcaccaacc ttcagagcac ttgagtcacc aaaagaggca actgtttcga ctttgtctga
    41941 attgatcttc aacaccgcta aatctgaata agcatctgtt ccaactaatt cagcttttac
    42001 cttcgtaccg tctttcatca agacttcaag accttgctgg ccatctacta cgtgattatt
    42061 cgtgactaca taagcagtat tcccgtcttt tttgtagatc acgccgcttc cttcactata
    42121 agcttccaaa ttactatcat ctgagctgct actttcttct tgctgcccga atagtccgcc
    42181 aaaaccgctt gattggtttt gactttgtaa gttgataaca gaaacgacag cgtcttgaac
    42241 tttatctacc gctttggtga tatcagaatc tacattgact ttgacatttt ccacaactgt
    42301 ttcaccggca gaattctgat tgccgcttgt agcagtagaa ctattgttgc cagtgcccat
    42361 cgctgcataa aaaatgccag ctgtcagtag tcctccaacg atccctccaa caagccccag
    42421 acctaactta cgccagaggc tattattttt actttttttc atctttggtg tcacattttt
    42481 tctatccatg tgttcctcct ccaattacat tcatttatct actgttagta tagtcttatt
    42541 tgttgacttt agtctacttt gagactgtga actttttatg aaaaacttta ggaaaaatca
    42601 ttgttttcag atgaaaatat ttg
//
LOCUS       DAYYGS010000014        41706 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000014 DAYYGS010000000
VERSION     DAYYGS010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.014"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA44_000714"
     CDS             complement(12..617)
                     /gene="dhaL"
                     /locus_tag="ACHA44_000714"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFM8904029.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA44_000715"
     CDS             complement(645..1655)
                     /gene="dhaK"
                     /locus_tag="ACHA44_000715"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFM8904030.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA44_000716"
     CDS             complement(1630..2025)
                     /gene="dhaM"
                     /locus_tag="ACHA44_000716"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFM8904031.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2179..2499
                     /locus_tag="ACHA44_000717"
     CDS             2179..2499
                     /locus_tag="ACHA44_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFM8904032.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2611..3468)
                     /locus_tag="ACHA44_000718"
     CDS             complement(2611..3468)
                     /locus_tag="ACHA44_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8904033.1"
                     /translation="MKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTISSSG
                     AENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELEEALE
                     AAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQQAKE
                     FNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAASFQRI
                     KDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGVLVAA
                     AYGIIRKKK"
     gene            complement(3576..4880)
                     /locus_tag="ACHA44_000719"
     CDS             complement(3576..4880)
                     /locus_tag="ACHA44_000719"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8904034.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4877..5386)
                     /locus_tag="ACHA44_000720"
     CDS             complement(4877..5386)
                     /locus_tag="ACHA44_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904035.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5561..7696)
                     /locus_tag="ACHA44_000721"
     CDS             complement(5561..7696)
                     /locus_tag="ACHA44_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFM8904036.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7779..9434)
                     /locus_tag="ACHA44_000722"
     CDS             complement(7779..9434)
                     /locus_tag="ACHA44_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8904037.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9436..9750)
                     /locus_tag="ACHA44_000723"
     CDS             complement(9436..9750)
                     /locus_tag="ACHA44_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8904038.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9920..10180
                     /locus_tag="ACHA44_000724"
     CDS             9920..10180
                     /locus_tag="ACHA44_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HFM8904039.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10194..10508
                     /locus_tag="ACHA44_000725"
     CDS             10194..10508
                     /locus_tag="ACHA44_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HFM8904040.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11238..11792)
                     /locus_tag="ACHA44_000726"
     CDS             complement(11238..11792)
                     /locus_tag="ACHA44_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFM8904041.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11918..13847)
                     /locus_tag="ACHA44_000727"
                     /pseudo
     CDS             complement(11918..13847)
                     /locus_tag="ACHA44_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14153..14869)
                     /locus_tag="ACHA44_000728"
     CDS             complement(14153..14869)
                     /locus_tag="ACHA44_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8904042.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA44_000729"
     CDS             complement(14870..16693)
                     /gene="glpO"
                     /locus_tag="ACHA44_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HFM8904043.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA44_000730"
     CDS             complement(16707..18203)
                     /gene="glpK"
                     /locus_tag="ACHA44_000730"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFM8904044.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18339..19799)
                     /locus_tag="ACHA44_000731"
     CDS             complement(18339..19799)
                     /locus_tag="ACHA44_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904045.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19789..21084)
                     /locus_tag="ACHA44_000732"
     CDS             complement(19789..21084)
                     /locus_tag="ACHA44_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8904046.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21416..22093)
                     /locus_tag="ACHA44_000733"
     CDS             complement(21416..22093)
                     /locus_tag="ACHA44_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HFM8904047.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22123..23214)
                     /locus_tag="ACHA44_000734"
     CDS             complement(22123..23214)
                     /locus_tag="ACHA44_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HFM8904048.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEKAYKELYLPSPLYNFLKARYQ"
     gene            complement(23315..23749)
                     /locus_tag="ACHA44_000735"
     CDS             complement(23315..23749)
                     /locus_tag="ACHA44_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8904049.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24333..25790)
                     /locus_tag="ACHA44_000736"
     CDS             complement(24333..25790)
                     /locus_tag="ACHA44_000736"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8904050.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25787..27682)
                     /locus_tag="ACHA44_000737"
     CDS             complement(25787..27682)
                     /locus_tag="ACHA44_000737"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HFM8904051.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27740..29515)
                     /locus_tag="ACHA44_000738"
     CDS             complement(27740..29515)
                     /locus_tag="ACHA44_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8904052.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29565..31061)
                     /locus_tag="ACHA44_000739"
     CDS             complement(29565..31061)
                     /locus_tag="ACHA44_000739"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8904053.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31254..32876)
                     /locus_tag="ACHA44_000740"
     CDS             complement(31254..32876)
                     /locus_tag="ACHA44_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8904054.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33204..34517)
                     /locus_tag="ACHA44_000741"
     CDS             complement(33204..34517)
                     /locus_tag="ACHA44_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8904055.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34762..34986)
                     /locus_tag="ACHA44_000742"
     CDS             complement(34762..34986)
                     /locus_tag="ACHA44_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904056.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(35006..35428)
                     /locus_tag="ACHA44_000743"
     CDS             complement(35006..35428)
                     /locus_tag="ACHA44_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8904057.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35570..36280)
                     /locus_tag="ACHA44_000744"
     CDS             complement(35570..36280)
                     /locus_tag="ACHA44_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HFM8904058.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36298..36795)
                     /locus_tag="ACHA44_000745"
     CDS             complement(36298..36795)
                     /locus_tag="ACHA44_000745"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904059.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36832..37284)
                     /locus_tag="ACHA44_000746"
     CDS             complement(36832..37284)
                     /locus_tag="ACHA44_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8904060.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37419..38024)
                     /locus_tag="ACHA44_000747"
     CDS             complement(37419..38024)
                     /locus_tag="ACHA44_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8904061.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38474..39094)
                     /locus_tag="ACHA44_000748"
     CDS             complement(38474..39094)
                     /locus_tag="ACHA44_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8904062.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(39096..39965)
                     /locus_tag="ACHA44_000749"
     CDS             complement(39096..39965)
                     /locus_tag="ACHA44_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFM8904063.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(39988..40395)
                     /locus_tag="ACHA44_000750"
     CDS             complement(39988..40395)
                     /locus_tag="ACHA44_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFM8904064.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40414..41703)
                     /locus_tag="ACHA44_000751"
     CDS             complement(40414..41703)
                     /locus_tag="ACHA44_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8904065.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT        11759 a         8983 c         7022 g        13942 t
ORIGIN      
        1 ttttattgaa cttaaggttg attgatcaca tctaaaagcg ttgaaaataa aatggcacta
       61 gaagcagctc ctgggtcgat atgtccgatt gcacgctctc ctagatagga agctcgtcct
      121 tttttagcta ccaagacttt ggtgtgttct tttgcctggt caatgatttc ttttgttaaa
      181 ttccctgcct tcaatgcctc tgtgactggc agccaaacat ctaacatcgt tttatcgtca
      241 gcttctgcct ttcctcggct ttgaataccc gctgttccct gctcgatcac ttcgcctaat
      301 ttttcttgag aatcaataga atcgacctct tttgtcgctt tcatcatgtt catgaatgca
      361 gatccgtata acggaccaga agccccaccg actttcgata tcatcgccat agagaatact
      421 ttgaatgtat cgctgattgt ttcaggatga tcggcttgaa aagcttcttc ataagctgaa
      481 acccctcttc ccatgttatt tccatggtct ccatcgccaa tcggggtatc tagatcactg
      541 agatattgtt tgttctgatt gattgcctct gcaaattgcg tgagccattc ttggatttct
      601 tgaacggtta gtttcatatt atcacccttt tatcttgaga ttttttacca tgaaatcgtc
      661 tgtacattgc tttctaaaga atctttccat tgtgtctccg tcaagtcgat cagtgttaga
      721 gaaagacctt gcatatctag tgaggtcata aaattaccga cttttcggaa aactacattg
      781 acgcctcttt cttccaacag agctaaaaca tcattcatga acacgaactg ttccatcaaa
      841 ggcgtcccgc ccattccgtt gactagtaca cctgcttctt ttggtaaatt cgagtaatca
      901 tctaaaatct tagtaacaag ttcctttgcc agtactttag aaggctgcat cttttctcga
      961 cgatagcctg gctcaccgtg gataccaaca ccatactcga tctcatcttc tcctagttcg
     1021 aatcctggtt tccctacttc tggtacagtt gctgctcgaa gcgctagacc gatcgtctta
     1081 gttgcctgta cgaccttttc tcccagttct ttcaactcag ctagggaagc accgtttctt
     1141 gctgcatcgc ccacgatttt gtgtacaagg acagtacctg caactccgcg ttttccagca
     1201 gtatacgtac tattttcaac ggcgatatca tcatctacga tcacactttc tacttcaata
     1261 tcgtccatag cagctaattc ctttgccata tcgaagttca atgcatcccc tgtataattc
     1321 ttaacgatca gcaaaactcc ttttccacta tcggcttcct taatagccgt ctgaatctga
     1381 tctggagttg gagaagtaaa cacatcacca agcaccgctg cacttaacat cccttcccct
     1441 acaaacccgg catgggatgg ttcatgtccg cttcctccac cagaaaccaa accaacttgt
     1501 tccgctttct gattcttggc tacaacacgg gaagattcta cacgatgaac aagttctgga
     1561 tagcttttta ctaatccttc cagcatttct tcgacaatat tgcttggatc attcatgatt
     1621 tttttcaatt tattcccatc ctttctttcc ggcattttgt gcttctgtta agatttgctg
     1681 agtatcatct gttataccgg cagtgatggc tgctgcaaaa gctccttcca ctaaaggcgc
     1741 atccaccaga tgataacgtt gctgctgctc ttcttccaac atgtcttttg ccagttccga
     1801 gttcaatacc gcgctaccga tatccgcaaa aacaaaataa ctagctgcat cgcttgattc
     1861 attgactgct tctacgattt tcatcgggtc tgatccgatc tcgccattta ttccgccgag
     1921 agaaaaaatt tgcacctctt cggatttttg catctgctca atcatttctt taataccatc
     1981 tgttactttt tgactatgag aaataagcaa aatacttttt ttcacaagaa tcttcctttc
     2041 ttttacatag ttgctaacga ttggtttcgc tttcattaat gaacaattca agtatataat
     2101 aaatttctaa ttttgggaaa atatgtgtag tgcttcttga agtttgttat actttaatta
     2161 aaagagagga gtgatcgaat gattacaaca acaacgaaca gtgtagaggg aaaagccgta
     2221 gtagcttata aagggatcgt ttttggtgag gtcattactg ggatccatgc atttaaagac
     2281 ttggaggcgg ggttgcgaaa tatctttggc ggccgctcaa aaagctatga aaacgaacta
     2341 atgggtgcaa gagaagaagc acttgccgaa atggccgaac gtgcaaaagc attaggagct
     2401 gatgcgatca ttggcgtaaa aatggactat gaagtattag gcagcgacaa tggcatgctg
     2461 atggtcacat gtagtggaac agctgtctcc acgagataag taggggacat ctgttttaat
     2521 aactaaagag agatgggaca tgtagtggca ctcaagaatt agatttttca gtctaacttc
     2581 gaggtactct accttcccat cctctctttt ttatttcttt ttgcggatga ttccgtaagc
     2641 tgctgcgact aacacacccg cccctgctaa ggaataactg acagaagctg ttttttcatt
     2701 tgtttttggc aaagtctgac ttgttgattt ggatggctct gtcgtttgct gaactgtttc
     2761 tgtcgtgctt ccagaggtca ctgatttttc tcctgccgaa tgcaaaatat cttttattcg
     2821 ttggaaacta gctgctaagt cagctttttt tgcttcaaaa tcattagcaa ctgtttgtgc
     2881 ttcgctgatc tttccgtgga ttgcttgact ggccgctgtg atatccgctt gagtttgact
     2941 tagctttcca gaagctgttt gagcaacctg atttaattgc tgattgaatt cttttgcttg
     3001 ttggatcttc ccgtcaagat ctgtttggac agcttccgct tttgctttcg cttcggcaag
     3061 ctttttctca agttgtgcac ggtcatttcc cactttattt ttaagatcag ctaaatcttg
     3121 atcgattgct gcttggtctt ctttcaattt gttttttgca gcttctaatg cttcttcgag
     3181 ttcttttctt ttattttcca agtcttgcaa atgatcgatt tggtcagaag aggtagaact
     3241 atttattgat gtatcttctg aagatgaaat cgtttctcct gaattatctg tcggtacatt
     3301 ggaagtcatt tctgtttccg aggcaccgtt ttcagcacca gaagagctta tggttgttga
     3361 aggttctgtt tgagcaggtt cagaagtttc cgcaaaagct gtctgtccca ataagaaaat
     3421 actggaaaca gcaattggcc agattaacgg cttcattctt tttttcatca cttttcccat
     3481 ccttactact ttttaactaa tttcttatta taagcaaaaa tgtttggaag aaccataaac
     3541 acaacaggct gtttgtctaa aaaacggtgc atcttttatt tttttgtacg ttccgggctt
     3601 ttccatgtcc cgcttccata acctaaaatc gttttgactg ctggaatgaa agtcgtcacg
     3661 atcgttatcg cgttcactat ccagaacaaa agcatatata gtggtgcgaa aagaaggtat
     3721 ttcaattttc ttcgtcgatc atctacaatc agcgaagcta gcaactggaa aaatcctgcg
     3781 accatttcaa agcagatgaa cacaaaactg aatgtaaaag taatataaat ctgttcgtag
     3841 tttccttgat atacataatg cccgattaat ccagcaaaaa gaactacgga aatacaaaag
     3901 aatattgacc agacaatact caatgtttga tcaatgaaga tgatcgttct tccaatatgt
     3961 ttaaaaggat ggagcatcac ttttttgaaa ttcgtcagcc agacttctgt tccacctttt
     4021 gcccaacgtt ttctgtgtcg atatagcatc ttcaacgtca caggtacttc catgaaaaag
     4081 ataatctcag gagcaaaaac agataaccag tcatttaact gatgatccca agcaatactg
     4141 atgtcttctg tcgctcgatc ttgtctgaat ccgcccacat cgatcaaggc ttcttttcga
     4201 tacatcgtat ttgctccact gtacgcataa agaccaccga aaacagctga ctgtgttcgt
     4261 ttaatgatgc ccacgatact tgagaattca accgtttgag attttgcaat caatttggta
     4321 cgattttgga catccatatt tgcagtgacc gcagcaatat gtcttgcacc agggcgaatg
     4381 aaataattca catacctaat cagtgcatct ggctccggta ccgtgtctgc atcattgctt
     4441 aaaatcaatt tcccttttgc aaaggcaagt ccgatattaa acgcgtgggc ttttccttta
     4501 tttttttcga tccgaaccac acgaagatta gcgtattttt tcatcagtct agctaatatt
     4561 tctggtgttt ggtccgttga accatcgtct gtcactaata cctcatagtt gtgataattc
     4621 aatttcgtca tcaaatactc gattgtatat tcgatcacaa tttcttcatt atgggctggc
     4681 accatgatcg tgatgaacgg ttcgacactc aagggaacat ctacccattc tttttgctta
     4741 tgtttgtata aaaacacata acaccagaca ccaataaacc atacaaatcc gcctaaaatc
     4801 ggataagaaa cgagaaaaaa atacataata gacaaacaag tatctaagaa taaattacct
     4861 gtatgggtga ttgagatcat ttcagttcca ccccattttc tttatacaaa tctgcaatga
     4921 atgtcgtatc tagatttttc tcttcggaga cagaataaaa acggaccgtt tctctctctt
     4981 cttttgttcc aaaacgttcc gtatacactt cttcaagtaa ttcttgtctt tttttcaatt
     5041 tttcatcttc atattcaaaa gatttattta cttttttgta tagatttcga ttgttccacc
     5101 tagtcaatac aaatgaggta atcaggaaac tgaaaaaaat caatacaaaa aagccgatta
     5161 agaaatacag cattttcttt tctgataaaa aagcgtacaa ggaaaaatcc ttggctcttt
     5221 ctggtctcgt taatgggaaa aagacccata taaaaggaag aacaacgatc atccatccta
     5281 aaagagccac taacgtttgc caaatcttta accagtaatt accttttgaa aaatacttat
     5341 ctttcaccaa aattgatgca acttcttctc ttgttacttt tttcatatcc tcacctcacg
     5401 tttttttgtt acgacgtagg agtttaccat acatttcatt tcttttcaca taaagtgttc
     5461 ctaaattttt cctaagaaaa agaacaaaac atttctcatc agcaaatgaa aaatattttg
     5521 ttctttttat cagttgttct ttttattttt tatctcttaa ttatgttgct ctttccaata
     5581 cgacaatgct tttcattcct ctagatatcg ccgtgtacat ttgtctgcgt cttctttgct
     5641 catctccatc gttttcaaat ggatataaga ttacatgatc aaattccagt ccttttgcta
     5701 gactcaaagg aatgatttgg cagttttcta atccatatga atttttttta atcgattctt
     5761 gtagcgtttg ggtttcctct tctgtttggg tgataactgc cgctttttct ccttttagag
     5821 aaaccaaaat ctcagctagt attttcaaca attcttcaga ggatcgaaaa gagcggtatt
     5881 cgggttcttt tccttgcggt cggatcgata cgatgtcgat cttctcttga tccacagcat
     5941 aggttttgaa aagctctgtg attgctccgc tagaacggta actatttcta agatcatacc
     6001 gatggatcgg cagatggtag tcgtcgaaac aacgcataat atcagcaaaa gtagtggaag
     6061 aattaaagat tgcctgattc tcgtcaccta ctaaagtaaa gtgagttttt ggaaataatt
     6121 cgatcaatag acctaattgt gccagggtat aatcttgtac ttcatcaatc aatagatagc
     6181 gaaatgctgg tacatttacc ttttcaacaa gtaaatgacg aatagctaat ataatgactg
     6241 cttcatcaac agtacgtgtg ggttgccaag cataagctga gccatatcgt ttttcataaa
     6301 ttctttcaaa caactgatgc tcatccaccc atgccatttc ttcgatttgt ctggttattt
     6361 ttcgatattt tttttgtaaa agtttccttg cataagctac aatactttgt tccgaatcat
     6421 cagaaatcaa tttaccgaat aattccaact gcctatcttc tgtcaacgag agtacctgat
     6481 tctgcacagc aggacttttt gcttgtttca aaagatgatt cttccattcg ctcatcagtg
     6541 cctttttcgt tgcttgaagc ctatcgatca atttaggatg agcaggaact ttctcatata
     6601 ttttttcgat cgttttttta gaaaggactg tttttccatt tttagtaagt ccacggataa
     6661 aattcggatg atctaaaaac aaaggatctg attgttttag attatcgata aactttttgc
     6721 tacgcaaacg ttctgtctgc tccgatacat tttctcccgt gatacgtttg aaataagcct
     6781 cttcaccttc tatttcctct gctgaaagct gtgaaaccag ctgtatgatg gttagattca
     6841 gtggatttct ttcacctaat gatggtagga cattcgaaat atagtcgata aaccgatgat
     6901 taggtgatag aatcagcaag tcatcggatg tcatcgtatc tcgatataaa tagagcagat
     6961 acgcaatacg ctgcatgata gcagaggttt taccacttcc tgctacgcca tttaccaaga
     7021 tattttgact tttcacatcc cgaatgattg tgttttgttc cgattggatc gtagcagtga
     7081 tagccttcat ttcattggtc tcatcttggc ctaagacttc taataatacg tcatcttgaa
     7141 tagctacaga cgtatcaaaa aacttgacca aacgatctct ttccaaaaga aattgtcgtc
     7201 tttctttaat aaccgtatcg attttatttt gacgaaccgt ataagaagat ggaccaatca
     7261 tgttattgta aaaaagttcg gcaattggtg aacgccaatc ataaacaaga ttttcatctg
     7321 catcatctgt gaaatttgct gtaccgatat aaaaggattc ttttggctca ttatccagaa
     7381 agtctacaac gacttttcca aagtagggac ttttcaataa acgttcgatc tttgctagct
     7441 gattttctgc gtttttctgt ttgatattca tctgatcgat ttctctattt ttcatttcaa
     7501 tcatcgaaaa agtatccagg ttatctaagt aactgtcaaa attcaatcga agctcgccag
     7561 ccatttcttt caacgactgg tttccttcta tttttgccgt atccatcgat tcccgcagtt
     7621 cgtttttttt atcaattagc ttttgataca cgttcttcag atatgcttct tctaactgtt
     7681 tttgtgtgtt gaccatacac attctcctct ttttttaaag tggtggaacc gattagttta
     7741 cctcttttaa gagtcgaatg caagcttttt aacgagtatc atttgttagg atatatcagt
     7801 tcttcaggtc ggacagtttg atataaagaa aatgcaccat caaggttttt tacgtggtag
     7861 ccagactgct tcaagattct ttccgctaga tagctacgta atccgctatg acagctgacg
     7921 atatattctt gttggctgtc taattcattt aatcggtccc gaagttcatt caaagggata
     7981 tgtttggctt ccttgaattt gcccttttcc aattcttctg ctgttcgcac atctaataaa
     8041 atttttcctt ttgctagttc tgtcggcaat tcatgccatt gtacggtttc actaagccct
     8101 tctgcaatgt tcattgctgc atatcctagc atgtttactg gatctttggc tgagccaaat
     8161 ggcggtgcat acgtcaattc taattccggt aaatcgaaga tcgtcaaatg ccctttgatt
     8221 gctgttgcta aaatatcgat ccgtttatcc acaccttttg ccccaacacc ttgtgcacca
     8281 taaatctcgc cagtctctgg atggaagatc aacttcagta aaatatctgt tgcttctgga
     8341 taatagcttg catgatcttt tcctgataca tgaatgactt caaaagacag ttcgttttct
     8401 ttggctgttc tttcacttaa accggtggat gcggcagtca ttccaaacgt tcgtataatt
     8461 gctgttccaa taccgccttg atttcttcga gaaattccgg aaatcgtgtc cgcaacttgt
     8521 ctaccttgac gattggctgg tgaagcaaga gaaataagtg catcttttcc agtgatttgc
     8581 tgttttacaa cgatagcatc ccccaccgca taaatatcag gaatattcgt ttgataacgt
     8641 tcgtctacta ggataccccc gcgtatgcct aattcgattc cagcggtcac tgctagctgg
     8701 ttatcaggac gaacaccaat ggataaaata actaaatcag cagggatggt acgcccatca
     8761 tccagtcgca atcgtttccc gtggtcttca aatccagcga cagcatgaga agtaatcact
     8821 tctactcctt gatgagacaa ttctttttct ataaaagctg ccatttcttc atccagtgtt
     8881 ggaagaatat gtggtgcttg ttcgatcacc atgacttcta aacctcttct ttttaggttt
     8941 tctgccattt ccaatccgat gaatcctgca ccaacgatca cggcacgctt tggctgtttt
     9001 tctaatgcat gcatcacttt atcgatatct ggtacatttc taatagaaaa aacattgtcg
     9061 gcttctgtta tccctggaat cgatggaaca actggttttg caccaggaga taaaaccaat
     9121 gcatcgtact gttcaatgtg tttttgaccc tcatgtacga cttctaccgt tttgttttcg
     9181 ggaaagatgg ctgtcacttc atgatttgga cgcacatcta aacaaaaacg agcggataat
     9241 gattctggcg tttggacaag aagattttcc cgttcactga tttctcccga aagataataa
     9301 ggcaagccgc aatttgcaaa agaaacatac ggtccttttt caaaaacaac gatttctgca
     9361 tcttccatta atcggcgaag ccgtgtagca gcagacattc cacctgctac acctccgaca
     9421 ataacgattt tcatcttatt tccctcctac tgttcgatca aaccattgat tcatgccacc
     9481 acgtacattc actacatcat aaccagattt ctttaattct ttagctgctt gtttactcct
     9541 catccctgat tgacaaatca catagacggt cttttctttg tctccatcat atctgtttat
     9601 actttgtaat ggaacatttt tagctccttt tatatgcccg ccccggtatt ctgcaggtgt
     9661 tctaacatca agtaacgctg gattttccaa aagcttttcc tttaattctg ggactgttat
     9721 cgattttatt gattgaaata gcaaattcat tttctgcacc tcatttacat ttttataaat
     9781 acctcatggg gtatattaaa caggagcttt attaatatgt caaccagaat gactcttcat
     9841 ttttttcact actttttata tttgcataaa ataccgataa ggggtattat ctaattataa
     9901 aaatagagga gaatgtgtta tggagattga taataaaaat atcctgaacc gtttgaaacg
     9961 tacagaggga caaattcgtg gtatccaaaa aatgatcgaa gacgaaaaag aatgtatgga
    10021 tgtcatcact caattaagcg ctgttcgttc tagtattgat cgcgtaatgg gcatgatcgt
    10081 tgcagataat ctgaaaaatt gctttgagaa acctgatagt aatccagaag aacaagcggc
    10141 taaactagaa caagcgatca agatgattat taaaaagtaa aggaatgatt tcaatgaaag
    10201 tcgttttcca tattgatgaa ttagaaaaat ggtcagaaac agggaaaaac gtaaaaaacc
    10261 tgataaaagc ttctccagaa acgacgattg tcgtatctgt aaatggcatc gcgattaccg
    10321 gttatctaga ctctgccaat gcagaattct tagatttgca aggagtcgtt tttcacgctt
    10381 gcgccaatgc tatgcgtgct aatcatattt cagaaagttc tttacctgaa caagtaatcg
    10441 ttgtaccagc aggtgtcctt gatttagttg aactgcaatc ccaaggttat gcttatatca
    10501 agccttagtc agtagaggtt gcgacagaag tgatcagctt caagaaattt cggcttattt
    10561 ttcgaagaag ctacttctgg aaaaccgtga atcaaaggtt gtgaaaacgc tgtcctgcat
    10621 caagtaataa gaacagccaa acaaaaatag cttctcatat ttttcgtttg gttgggctta
    10681 ttaccgcaga tgcaagcttt tgaacaccgt ttaaggaaga ggttgtgaaa tcagcggttt
    10741 gacctaagta ttagagaaaa aaacgggaaa atagctcctc atattttgcc gttttttgag
    10801 ctaataccga aggtcagctg attgaacacc gtttaaggaa gagattgtga aaaagtcgct
    10861 tagtttcgag tattaagaaa aattttgtga aaatggattt ctacattttt gcataaattt
    10921 ggcttaatac cgagaaactg gcttttgaat accgtttaag gaagaggttg taaaatcagc
    10981 ggtttgacct gagtattaga gaaacaaacg gaaaaatagc tgctcatatt ttgccgtttt
    11041 ttgagctaat accgaaggtc agctgattga acaccgttta ccaagaggtt gtgagcctac
    11101 catttaactc cgaatcacaa gaaataattt caaaaacaac ttctcctgtt tttggttcgg
    11161 ttggcttatt atcgagaagt agctactggg agtctattta tcaaaaaaag gaatgcgaca
    11221 taggtcacac ccctttttta ttttctttga aacatctttc ggtactcgac agcatacaag
    11281 acaaaagatt gtaaaagacc gtaagtgatg acggcaaaaa tgactctcac ccacagcgtt
    11341 ggtaattctg ggaaaagcag caaaaggatc atgccaacat agatgacagc agtcgctact
    11401 ttgccatacc acttcgctcc acttaatttg atatttttag atagcccgat taatccagct
    11461 acaaacataa agccttcttt tatcaagaat agaccaaaca acagccaaag ccacggtcga
    11521 taccatgcca tagacaaaat cagaaaaagt tgtgtcaatt tatctgcaaa cggatcaagc
    11581 accttcccta agtctgttac ctgattatat ctccgagcaa tgaccccgtc tagataatca
    11641 gacaaacctg agacaacaat gaccgctcct gctatataaa atgactgtct cagcacaaaa
    11701 taaagataaa tcggcagcaa aaaaatacgg aatatcgata aaagattcgg aatcgtttgt
    11761 atttctttac gccagtctgt gcttaatttc attttatcgc cctacctcct cagcactctt
    11821 ttctttttat tttagcttac acggggaata aaaccaatga tttcactgtt tttatttttc
    11881 tttacagaca ctgatattat gagctaattt gagataatca cttgctgttt tcatcgttta
    11941 ctcttctttc ttcctgtcgt ttgcgcattt cttttactag tactttatga gctttttttg
    12001 atcctcgctc taggtcacgc tgccgtttac gtgttttata atcgataaac agactgtcta
    12061 aatcgtaacc tgtcgggtac agatcttctg ctgaacgcac cagacgaaca cgctgacgag
    12121 gcacgtggat tatttttttg tctaaatata catcaatcgt atcttcacct atatcttcat
    12181 aaactaacgc ttctttttgg tattcattga caaatatccg atcacctttt gcaaagagct
    12241 gacgttcttt tttattctcc ttcctatttg taactgctgt cttaggaaga aattcttttt
    12301 tctgtgtccg ataggctttt tccgccaagt aacgctctgc ttgatggatc aatttcatcg
    12361 acatatccat tttatgagca atccacaaag cttggctttc tcctgtttct tccaacccgc
    12421 aactgatatt taggacgaag tgcttcccga tcgaaagcca tagcagccgg cacaaaatct
    12481 tcatgatctc cggcgaattt cttgatttct ccataatggg tcgtggcaat gatcaatgcc
    12541 ccttgctcat acatagattc catgattgcg atcgctagtg cagccccttc atttggctct
    12601 gttccactgc caatctcatc taaaagtacc aacgtattcc tttttatctt tttcaaaata
    12661 gcagcgatgt tttgcatatg accagaaaat gtactcaacg cattttctaa attttggtgg
    12721 tcaccgacat cgacgaaaat ctcatcaaag acagcaagct ctgtcccttc ttttgccgga
    12781 acttgcatac cgaacatcgc catcaaagtc aataaaccga ctgtcttcaa cacgactgtt
    12841 ttgcctccgg catttgctcc ggtgataatc aagcctcgat agtctttacc gatttcaaca
    12901 tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc cttgcttgat aatgatatgt
    12961 tccgatttat tgattctagg tgtgcttcca ttgatttctc ggctaaattt tgcccgagca
    13021 aagatgatat ctaacaccgt gatcgtttcg atcaataaat cgatcacttc ttcgttttct
    13081 gcgatagcac cagtcaatgc cgccaggact tgatattctt cactgatttc ttcagctttt
    13141 aacaactgat agtgttcatt caatttagaa atcacagttg gttcaataaa aaccgtcgtt
    13201 cttttatttg attcatcgat aatcgttcca tctattttat ttttatagct ggctttgata
    13261 ggaatcgtat agtattcgcc tttttgaaca atcattgctt cttggatcat ttctttgttt
    13321 ttcggatgac gcaagaattt cagtaatttt gactggattt ctttttcgat tagttgtaat
    13381 tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac tgactttatt attttttatt
    13441 ttctggtcaa tcaactcttc tacatttatc aaatcaggaa gatgtttgct ataagcaaac
    13501 aacaagggtg tctggtattg attcttatca aaaaactttg tgatcatccg actgcttcta
    13561 agaaaatccg catattcgat caaatcggtt ggctgcagaa tcaacccttt tttgacttgt
    13621 tctgtcagtg catcgatcct cggcaacccc ataaatggaa catgctgact gctttctaag
    13681 atcaagcgtg cttctcttgt ttcttcttga cgatccaaga cagtttgtag gtttgtcgag
    13741 accgtcatat cgctgattcg ttttttgcta tagtttccaa ttgctcttgc ttggatttcc
    13801 tttttgatcc gatcaaattg taattgacta atcatttcgt tgtccatatt ctctcctttt
    13861 cttgaagaga tacctgtccc aaaataaata agggcctaga gataaataaa aggatagaac
    13921 acagcttaaa aaagtcatgt cctatcctta ttagaataca aagatattgt tttcctgaca
    13981 ggtaccgttt cataagcaca ccaaataaac tttgtttatt ggctattcga cttactgatt
    14041 agataccagc tacactcaca aaacataccc cattcttcta ggtcttattt attttccact
    14101 taactatagc aagacacatt ttggttgtca aactttagta tgttttatag atttacacca
    14161 ttaatgaata gacacctacc gcgataattg ctccgatcat tggacctgcg attggtaccc
    14221 atgaatatgc ccaatctgaa gttcctttat tagctattgg caataccgca tgtgctaatc
    14281 gaggaccaaa gtcccgagca gggttgattg catatcctgt tggccctcct aatgaaagac
    14341 cgatggctaa gatcaatcca cctacagcga atacgttcgt tccaggtgca aattcgtttt
    14401 gtccaaaagc tagcaaccca agaactaata caaatgtacc aattaattct gtgatcacat
    14461 ttgctggata attccgtata gcaggacctg ttgcaaacgt tcctaaaatc gcgctttcat
    14521 ctttcgttgc attccaatgt ggcaaataag aaagccaaac aagaatggcc cctgcaaaag
    14581 ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt ccaactaatt gcccctgtca
    14641 tcgccatagc tagagagact gcaggattca aatgagctgg gcccatgaat ccagaaatat
    14701 agacagccat tgttacagcg agtccccaac caaatgcgat gacaatccag ccagaagcct
    14761 gtgctttact tttgtttaaa ttcacagccg cacacacacc atctccaagc aaaactagga
    14821 tcagtgtccc aataaactcc cccatgattt gagtcatatc actgttcatt tatttctccc
    14881 cctttagttc ccgcagatct gattctgcaa tcaatgcttc taaacgttta gtctgttgcg
    14941 ccttctcctc ttctgtccat tcaaaatatc cagcaattgc atcgatcaca ggttgcttga
    15001 tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt acgacgaatg agataatcgc
    15061 ctggggccaa aaccatttct tcttctaaac cataacgtaa ccgagctgac tcagcgagac
    15121 tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa gatacgtcgg gcattagtcc
    15181 cgtaaaaatc agcgatataa gtagcatctt cttcctctaa accggctgct actccttctt
    15241 ttgctaattc tgtcactgtt tcttctaatt tagttggatc gaagtttcca cctgaaatct
    15301 gatatttttt agagtcgatt tctttcgtct ctattccgta ttcttctttt aataattggc
    15361 gtatcaatcg caaagctcct tctgccatct tacggtaatc ggtgattttc ccaccagata
    15421 aagtcagcaa accatctggt tctctttcta aagaactacc tctggaaatc gtagaaggtg
    15481 ctttttcatc acggctgttt tccaaatggt tcaacacgtc ttctacttca gcacgagaaa
    15541 ctttgttttc cttatattca cttactgtat cgacaacttt attgaagctc ttgtctgaaa
    15601 tcgatccatt atctccacca ttgtaatcag aaccagaatt accaatcaac agtggacgaa
    15661 gtcctgccca gcttgcttcg atatcattca atgtgatatt tgcttctgga tagcgatgat
    15721 tgatcacatc caatagataa tctacatctt cttgtgtgac tttcggatcc gtaaaatctc
    15781 cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt ttctcttgga atagcaaaaa
    15841 ccatccgccc atcttgcttt cctgtatcga agtatgtggg ttgcggtaca ggcagttttt
    15901 tcgcatctac aactaaatgg atccctttag ttggacgcat tttaggagag actggacgcg
    15961 taaaattcaa gtttcgtact ttatctaccc aaggaccact cgtattgatc actaattttg
    16021 ctttgatttc gatcacttca tctgtcagca ggtcacgagc tttgacgcca acgatttgat
    16081 cgccatcata taaaaagcca accgctttca ttttacttac tagataagcc ccatcttcgg
    16141 cagccttttt gatattatca atcactaaac gggcatcatt gttgcggaaa tctagataga
    16201 cacccgcgcc ttttagccct tcttttttca aaaacggttc tctttccaat acttcttctg
    16261 gtgtcagcgt ataattctca tatttggttc ctgtcacatt tgccagtttg tcataaaggt
    16321 ccattgctac tttgaccgag aacatattga aggttgttgc tccttcgccc tcatagattg
    16381 gcaaaagcat tggatctggt tttgggatgt gtggggcaat actttgtacg actgcacgtt
    16441 caccaactgt gtcagctact acttccacat caaatgtctt cagataacga ataccgccat
    16501 gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa atcctgcatt tcgatcaatc
    16561 ctgttttgat tcctgatgct gctgactgga ctgccacacc ggcaccagtg attccgccac
    16621 cgatgatcaa cagatccaat tcttcttgct gcattttttg aatgctatct tgtcttgtct
    16681 tgtttgaaaa catatggatt tcctccttat tcaaatgctt gggtagcttt tactgctttt
    16741 ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca tttgaacttc aaatagcttg
    16801 ccagcttcat aaaattcgcg gatctcgtct gtatctttcc aataaccgac agctaatcct
    16861 gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt ctgctcgttg gatcggtaca
    16921 ttcaagatat ctgtttggaa ttgcatcaga tattcattgt ttgctgcacc gccatctact
    16981 ttcaatacag gaatcttgat tccagtgtca tcttgcatcg tatcgatgat atctctcact
    17041 tgataagcaa tcgactgtaa agtagcttta atgatatctt cttttgtcgt accacgtgtc
    17101 aagccaaaca tagcccctcg tgctgcctga tcccaataag gagcacctag cccaacgaat
    17161 gcaggaacaa cataaacctc atcttcgctt gtagagcgtt tcgctgcgtc ttctgaatcg
    17221 ctagcttttt gcaacatttg aagtccatct ctcagccatt gaatcgaaga accagccaca
    17281 aaaatacttc cttctaaagc atagtaaacc tttccattta tgccgtaacc aattgtcgtc
    17341 aataaattat ttttggataa ttgcggtttt tcaccggtat tcatgacaat aaatgaacct
    17401 gtaccataag tattcttgac catgcctggt tcgaatgcca tttgtccgaa taatgcggct
    17461 tgctggtctc ctgccatccc tgaaattggt gcttcgcttc cataaaaatg ataaccttgc
    17521 gtataaccat aaacttctga gttagaagtg acttttggta acatcactcg aggaatattt
    17581 agtaaatcta aaatctcctg atcccaatct agctgatgga tattgaacaa catcgtccga
    17641 ctggcattcg agtaatcagt aacatgtgct tgtccatcgg tcagtttcca tactaaccaa
    17701 gaatcgatcg ttccaaacat caattcgcct ttttcagctc gttcttgcgc gccttctaca
    17761 tgatcaagaa tccagcgtat cttcgttgct gagaaatagg catcgatcac taaccctgtt
    17821 ttttcgtgga aaaattcttg atagccttca ttttttaagt tatctgcaat tccagctgac
    17881 tgtctggatt gccaaacaac tgcatgatag attggacgtc ctgtatcttt ttcccatatt
    17941 actgttgttt cacgctggtt cgttatgcca atcccagcaa tctgatttgg tttgatccct
    18001 gattcgatga acgctccggc aataactgac tgtacagaat tccagatttc atttgcatca
    18061 tgttccaccc aactttcttg cgggaaatat tgcgtgaact ctttttgaga actgccgatg
    18121 tgtcttcctt tcttgtcaaa gataattgct cgtgagctag ttgtaccttg gtcaatcgcc
    18181 atgatatacg ttgattctgt cattgtgatt ccctccatta tatgcaaacg ctttctttaa
    18241 ctactcttat catactactg ttttaaaaaa agaaactgtc aaaattttat tctttttttc
    18301 tttttttgac agttttttat tatgaagcta ttttttaatc aattcttttc ttttttcagc
    18361 gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg acgcaaattc tgacagataa
    18421 tattcacact gttttttgtt tctctggcaa aatgtgatca ccaaatcata ttctttacct
    18481 tcttcataac ttccaatctc aataccgccg attcccctca gttctctgat cagatattgt
    18541 tgaaaagcaa tacgataaat cggcagactt tctaaatcga ttccaatcac tactggtttt
    18601 gcaatataaa aatcgatcat caaaagaaca ttggcatact ggatcatcag gtaactaagc
    18661 tcactatctt cagctctttt cctcggcaat tgctgcaccg ctgtttcctg taatttcgcc
    18721 agtaactgtg ttaaggaatg cccgagcatt ttctgctgct gttccaaaag ccgatcacgc
    18781 tcatagactt cgatctttcc aacaaagaaa aaccattcat tgttgatctg ggaaatttgg
    18841 tagcctactg ccttttcttc tttgattttc aaacgacgag gacgataatg ccataacatc
    18901 gtttcccgaa tgtaagtatc taaaacagcg gttggtaact ttttgctccg tgtcaaatca
    18961 tactgataaa atatttcttc gtctactatc gaaaaagaaa caaaaaagct gtaaaacatc
    19021 attgtttcgt atacattcag ttctgctgct gttcgactaa gatgcatcac gatgattgga
    19081 tcgattgctt gataaagccg gtccgactga tagagtaatt tcttttcttt caaagttgcg
    19141 tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc cgagccagca agcaatctgt
    19201 gcttctgctg atgccgtaaa cgttgtttct aatacacggt ccaatcctag aatgaacggt
    19261 cttttttctg gtgtattttg caaaaaaagc ggtcgctgat cttccgggat atattggaat
    19321 agttcataat aaaaatatcg tatctgtagc tcttctccgt ataattgacc gtttttgatt
    19381 tgtaatccaa attcctctaa gagctggttc aattctttga tctttcgaaa aacagaagac
    19441 tcactgatat taaacgctgt cgctaatcga acgatggata cttgctgatg ttctaataaa
    19501 tagacgagca tcttaaactt caagctttcc tgcaataaaa acaatagaat tttttccaaa
    19561 cttacatctt cgtccaacac aagcagcgcc tgatactcat ttcgctgtac ttctactttt
    19621 ttttccatca ttcggccaat caattcaata tcttccaaat attgatcaaa cgaggctttt
    19681 gacagttgga catgctctct caaaccattg atagaggctg ttcccccttg cagaacaagg
    19741 tttcgcaaaa tcaggatctg taactgctct tttctttcta ataatgcttc aatcttcata
    19801 ggcagtattc gccacctttg accacaccat atctggcaca cggtattctg gctggaaaaa
    19861 tagctcatgt tcttctaact gttcaaacgt ccatccttct tctactgcaa ttgtcagttg
    19921 gtcaagaagg tggaacatct cttttttgct ttggagctgt gctccaagaa tctttttttc
    19981 agaatcaaat agaaatttga gtttaacttt gctttgattt attgctgtaa gcttgtagtc
    20041 tacgtatcct gtttcaattt tttggggata aaaaatgctc tcttcttctg tcaaacctac
    20101 gcttcctaag aaatagccaa accactgatt gccgactgga cgataggttc cctgatcctt
    20161 tgtctgtgag ccactaattg tcttgctgac atttcgtgct gtacggacag cgttatttac
    20221 tagagaaaca tacatttttt catttgttgg acgaaaagtt acttggatcg catcgccaat
    20281 cgcataaacg tctttttgag acgtttgcaa gtagtcatct acccagatcg ttccatcatc
    20341 attcaatgtt aattgttctt cccacatctg attgtcggga cgagaatgat tagccagcaa
    20401 aacaagatca tagttctcct ttcttttttc cgtctctaca atagcggttt cattttcggt
    20461 caaagacaca acttgttctt ctaaaaaaag tgaaagccct tgatttttca tttcctttac
    20521 taacggttct gtcatttcag gatctagata acgaaaaaga attgactttc ggctttcgta
    20581 aagatggatt tgtttttttc ttgatgccaa cccttcagct atttcgattc ctgcttgtcc
    20641 tgcaccaata attgcaattt tctttgcatt catgatcttt tgctgaagag ccgttacgtt
    20701 ttgtatcttt ttaactgggt gaataagcgt tgattctgca gaaatatttc gaaacttttg
    20761 atttgaacca gtcgctagaa tcaaacgatc aaaatccaat ttttttcctc ctgctaaaac
    20821 gacttttttt tctttcaatc ctattactgt cttttctgtg taaacatgga ttcgatagcg
    20881 gtcagtcagt tcttctttcg tgatccaaga aagttgtttt tcactggtaa cctttccttt
    20941 taatatcaaa ttgaacccgc tgggaatcca tcctaacttc gattgctttt caatcaaata
    21001 tatttcactt gttggcatca attggcgaag ataaatagcc gcatgaatcc ctgcaaacga
    21061 cccgccgaca attacaatgt tcatctgcat tcctctttct tataaggtgt tctactgcta
    21121 acttctcaat cagaaaacac agtattcata actcgctttc aaccttatga tacatagcga
    21181 tgcagcagaa tcaaccctct ccatcatacc gtgttctagg tctgcttctc aataatgtac
    21241 tagtggacct aattgaagag tatcatttct gaagagatgt ttcaaaaaat gtactcttat
    21301 aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat aggaaacaga aaagaacgaa
    21361 aagacgagtt ttagcgtgtg taatgtaata gttagaatag acacatcatt tatttttaat
    21421 tctttaatgc ttcaacaact gcttcgccta attgatgagc agattgagga ttttgccctg
    21481 taagaagccg cccatcaatt tgaaccactt ctgaaaaagc tgctccagac tgatagtttg
    21541 ctcctttttc tttcaaagca tcttctgcta agaatggtac ttcattcgtt gtaccatttg
    21601 cttcctcttc ttcattactg aacccagtca ctgtacgtcc agctaaaata gattttccag
    21661 actcatctgt aactggcaac aatcctacaa caccgtgaca aacagctgag accagccctt
    21721 catttcgata gatttgtgaa gcgactttcc caagatctat tgcttctgga aaatcccaca
    21781 ttgttccgtg gccaccagca taataaaccg ctgcataatc atttgcatcc acatcatcag
    21841 gacgtaaggt atttcccaag gcccgatttc tgaaatcttc atcttcataa taggcccaat
    21901 cgatttcatc catattcaaa acactttgcg gttctaaagg tacatatccg cctttaggac
    21961 tgacaaaatc tgcatcgatc ccatttttta ctaatacatc ataaaaatga gtcagttcac
    22021 ttaaccacaa acctgtagga cgctcgatct tttcgtactt ttctacattt gttaatacga
    22081 tcaatacttt cattttccca tcctctttct ataaaaattg cgttattgat aacgtgcttt
    22141 caagaaatta tataatgggc ttggcaaata aagctcttta tatgcttttt caaaaggttt
    22201 cgttacaaat tcatcctcac ggttttccaa taatttctct gcccagtcag gatcgacgat
    22261 catttgctgt ccaattgctg caagttctgc atatttcaat acctgctcta catcttcgcg
    22321 attgcggatc ccaccgacac caactaaagg gatctttcct tgaatcgtgt tatggaccac
    22381 ttcaagaatc gatttatcgt tgtatttatc agaacgagct attcgatcat acatatttaa
    22441 tgaaacatgc agataatcta gttttgactc acgtaatcgt tcaagaagcc agatcgtgtc
    22501 ttcaaaacgg atacctggtg tttcatattc ttctggtgag aaacgatacc ctacgatgaa
    22561 tggtttttta gcatattgtt caaccgctga gaaaaccgtt gttaataatt cttcaataaa
    22621 acgataacga ttttccaagc taccgcccca ttgatcctct cgtctattcg aatgcggaga
    22681 gaaaaattgt tggatcaaat acgtatttgc tccatgcaac tcgattccat caaatcctga
    22741 ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt gtttcaacga tttcttcttc
    22801 actcattgct cgtggtgttt ccgcatcagg acgttttgct gcaactgcac ttgcagatac
    22861 aggctgttct ccagataaag ttgctcgact tgtcattcgt ccaccgtgga aaatctgaat
    22921 aatcgctttt gcgccttctc cttggattgc tttcgctagc tcacttaatc gaggaatcat
    22981 ttcatcatga gcgatgctga gttcacctgg ccagcctttt ccgatatctt ggacatttgc
    23041 agcaccagta ataacagcac caagaccttt agaacgttgt ttataataag tgatctcatc
    23101 ttcggtgatc actccattga aaaagctgag ttgtgtcgtc ataggcgaca gcatcaatcg
    23161 gttcttcaat tgaactcctg aaggtaaagt aattgattga tcaaattgtg acattgtcat
    23221 tcctcccata attcgctctg attaacctat cgagaatcat ctatcattaa accactcgat
    23281 tagttgtaca tgcaactaat ttgcttaaaa aattttattt tttttgtaac ttatctgttg
    23341 catcattcat tgtctgggct aattctttac ttgcttcatt tcccaaaact gctttgtatg
    23401 actgatgaag ttcttcccag ctaccatcga cttccttagc aaattggcgt ccttgttctg
    23461 taagataaac taatgacaag cgaccttcgc taactgtttt gacgtatcct ttatattcta
    23521 atttttcaac aaaacgagca atcgttgatg gggcggaata gagcatttgt gcaatatctt
    23581 tttgtggtat tccatctttt tcttttaata tcaataaaat aaaaccataa gttggtgcca
    23641 gacctgtcct tctaaacgct tcatcagcta atttattcaa catgcgatct agctttttca
    23701 cgctaaagaa aagacattca tctaacgctt tttcaattgg cttttccatt tcatcccctc
    23761 cattggcgta catatcatag tatgattttt attttttgtc aaaaaacacg gaataatgtc
    23821 cgcctcctct gtctagattt gaaaaaaaga agtagccatc ttcaaagaat ttcggcttgt
    23881 tttcgacaaa actccttctg gaacaccgtg aatcagaggt tgtgaaaaag tcgtttagtt
    23941 ttgagtatga agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    24001 aataccgaaa aactggcttt tgaacaccgt ttatatcaga ggttgtgaaa aagccgtcct
    24061 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atattttgtc gttttttgag
    24121 ctaatgccga tggtcagctg attgtacacc gtttattgag aggtcgtgag cctgccgttg
    24181 aactccgagt cacaaggagc aatttcaaaa aacagcttct catgttttat gactatggtg
    24241 acttgtgatc gaggagttgg caggagaata ctgtttattc gtaaagaagg ggctgtgaca
    24301 agacttttgt cacagtccct tatttccttt tattatagga tttcgccatt tgattggatg
    24361 acttgtctgt accaatcaaa tgattttttc tttaaccgtt tctttgtgcc attcccttcg
    24421 tcatcttgat caacatagat atatccataa cgttttgaca tttgtgaagt agaagcgctg
    24481 acgatatcaa tgcagcccca cgacgtataa ccaaataaat ctactccatc tttgatagct
    24541 tccttcatct gctcaatatg ctgcttcaaa tagtcaatgc ggtaatcgtc ttgaacggtt
    24601 ccatcttctt ctactttgtc tatagctccg agtccgtttt cagctatgaa cagtggtttt
    24661 tgatacctat catataattg attcaaagca attctcaaac ccttaggatc aatctgccag
    24721 ccccattcac ttgatggaag atacggattt ttaattcctc cagttattaa gtttccttgg
    24781 accccttccc aattttctgg atgagcactg actgcactag acatgtagta gctaaaagaa
    24841 acaaaatcaa cagggtaggc tgctaagatt tcttcatcac ctacttctat ttttaagttg
    24901 acaccttttt cttcaaacat tcgttgcgta tacgctggat aatatcctct tgcttgaaca
    24961 tcactaaaga acaaagaagt tctttgctgc tcaattgttt tttgaatatc atccggatca
    25021 caagaatacg gaacaagaag ctgatagctg atcatacaac cgacttgtga acctttgata
    25081 atttcatgac aagcttttgt agctaaagca cttgctataa actgatggtg tgcaccttgg
    25141 tatttcgctt gttccaaatg gggatggttt tcttcaatta ctcctacgct aacaaaaggg
    25201 tgatgtttta cacagttgat ctcattgaaa gtcatccaat attttacttt atttttatag
    25261 cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa taagctttcg attaggccat
    25321 ccaccatatt ctgtaaccaa atataacggc atttcataat gggaaagaga aacgactggc
    25381 tcaatcccat attttagaca ttcgtcaaac acttgatcat aaaaaaataa tcccgcttca
    25441 ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc atgcgataga catccgatag
    25501 catttaaaac ccatttcagc aaataaacgg atgtcttctt taaagtgatg atagaaatca
    25561 tttccgtgtc ttttaggata ataatacttg ctgttgggat cctgcgccgc ttttacttgc
    25621 tgatgcgtca tcatatgcca ttgatctgtc catttttctt taggtaaatc gctgtcgaat
    25681 gtatacacat cagacacaga taatcctttt tcatctacat cccatgcacc ttcacattga
    25741 ttggccgctg tcgctccgcc ccataaaaat ccttctggaa attttttcat atcgatcccc
    25801 ctaaacttgc tagttttctt gtttgattat ttttcttatg actagtaatg atatttttga
    25861 atctactact atttgtaaca ataaccattg tggtcggatc atatccctct ttgattaatt
    25921 cttgcaaatc tacttccatc aataaatcgc ctttacgtac tttttttcca tcagatactg
    25981 cactttgaaa gtatttgcca tttaaattca ctgtatcaat accgatatgg atcagtatct
    26041 ctgctccttc ttcactgact accccaattg catgtttcgt tggaaaaact gttgaaactt
    26101 ccccgtttac agaactgtag atttttccgt ccgttggaat gatcgcaaac ccttctccta
    26161 aagatccact agcaaatgtt ggatcgttta cttcagataa atcaattatt tcacctgctg
    26221 caggagtact taacactata tcctttgagg attcttcttc gatttcatcc ttgtagccga
    26281 aaagaaaagt tagcatacag gaactgatca tcgctaagaa ataactagca actagccaaa
    26341 caaatccttc accaatataa atcgggaaag acaaaatact tcttgtagca actgccaaag
    26401 caccggctcc tgcggcacca gctactccac caaatatagc tccacatata caagcaatgt
    26461 aaaatggtcg tttatattta agagtcacac cataaacagc aggttcagtg attccaaata
    26521 atgctgtgat aaatgcagat aaagataaac tttttacttt cttatttttc gttttcaaaa
    26581 ataccccaag tactgctcct gcttgcgcag ctacggcagt catacatgct ggacctagcg
    26641 tatcacgacc gtagaaagaa agattgttgg tcattactgg tacaagtccc caatgtaacc
    26701 caaaaatcac taacacttgc caaaatccgc cgataacagc accagataaa acagggctaa
    26761 agttataaac agctaaatat ccagctgcaa tcagctgact tgcataatct gcaattggac
    26821 ctacaactaa taaaataatt gggaccatca cgatgataca aatgaatggt gtcaataaat
    26881 ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc aatataagaa agaacccata
    26941 cagctaaaat tgctggaata accgtacttg tataccttgc tagaatcacg ggcatcccta
    27001 gaaatcgcaa ggttatgcta tcactatatg cactgacaat cgtaggataa acgagagctg
    27061 ctgcaataac catagcgatg aaacgatctg tattgaactt ttttgcagca gtatatgcca
    27121 aaaatatagg caagaaatag aaaaatgcat cagctgctgc attcaaaatg atataagttc
    27181 ctgattcagt agttaataaa ttcaaactcg tacataaggc gagtactcct ttgatcaaac
    27241 cacttcctgc caagacgcct aaagtcgggg caaaaacagc ggcgatcatg tctataaaag
    27301 agtttaaaat gccttttttc tcagttcctc tctttccact agatgcatca tcttctagtt
    27361 tcatttgttt ggataatacg tcatacactt catttactgc atttcctact acgacttgaa
    27421 acattcctcc agcttcgact acagtgatca caccttctag ttgctctacc ttctccttat
    27481 ctgccttctt attatctttt agggtaaagc gtaaccttgt tgcacaatgg taaacattgc
    27541 tgacattttc ttcgcctccg acaaatcgga caatatcttt cgctaattgt gaatagtcca
    27601 tttttacttc ccccttgtta attaatgccg tagcaatcct tcttacaaga taagtatagt
    27661 tcttagaaaa cggtttctcc attcaattag taccccttct aatagcgaac gaaactgtac
    27721 aatagtgtgt atggtttact tagccgagca aatcactcac tgcttgaaag aaaacaagta
    27781 tatcgtttct cttatagtca aaggagacaa aaaaaacacg cttgatcttc ctttgctgat
    27841 gtataagtgg tatctcaagc tgatactctg tgatttttgt ttctacttgg cttttgataa
    27901 gacagcacaa caaagtctcg gcatttaacg gaagcataac taggtcagaa ctaggagaga
    27961 ttagttgctg aatggaatct atctttttat ggatttttat ttgattttct tctttgtagt
    28021 aatgatcaag cagttttgtt ttcttttttt cttcaactat ggtcagcttt tcacgcaaaa
    28081 tctgataaat ctcccaagat tcaaactcat ctagaacact aggtagataa atcaaatcat
    28141 cccactcaaa aataagctct tgctcagtag tcaaaattgc atcatattcc gttattttgt
    28201 ttgattgatt tttgaaaaga tacactggtt ctatccggca ttctttgatc cacatgttca
    28261 tactttgttg aatctgtttt tttagtgtat tcaaaatact aattggctgg ttactaacaa
    28321 tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc ggcaagatac aaaacaaaat
    28381 aagcgctttc agctaaattg ggacgtaatt gaaaatgtat tgggaagaaa cgccgaatta
    28441 aataaatttc taaaggataa cgggagatga tcttttgtac aaaatgattc atggtattat
    28501 gtccggcatc catcctcgtt ttcatttgtt tgatatgggt tagcaaaaat tctgtttcat
    28561 ggaacaataa cgaaacagga agatctaaaa aacgaataat cgtttgctca aatgctctta
    28621 gattcttctc tatttctttg tttttttcat cattaatcat caaataattt aactctaata
    28681 gtcgattttc aattgcttgt ttttcttgat tggaaaaaga atagggtagt tctgatgata
    28741 acttttttgt gagggtctct accatcggaa taaataatgt atacctgttt gttttttcta
    28801 aaagttcttc tcttcgaaga atggtcaaaa cgagataccg agaaaatcgt atgaagtcct
    28861 ctcctgaaac aatataatta tatgatataa gaacctcttt taatgtcttt tggacagtcg
    28921 gcatgatatt ggaaaaaatt gtactgattt gttgaggaaa ccttgcgtgt tggatcaccg
    28981 tttctgttcc aacaagagat aagaaaattc tttttgaatt ttcttcagca atgattttta
    29041 gtccttttgt cgacgaaact tccagttcta atttaggatt gcgctcgatc caacgcaaaa
    29101 tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa gaaaatgtct gctatctttt
    29161 gcatactgac ataccctttt tgaaacaata agtacgtcac aatagttgaa gctcggaaat
    29221 ccatgctgga atagatttta tgtttcgtca cctcttgtat gatttgctgt ttagtttcag
    29281 attcaattga ttctaaaaaa taacctgaac gtttatttga ctggataatg attttcttct
    29341 cgtagatagc aagcatattt ttgatttcag tttggatcgt tttcttgttg gtacctaaag
    29401 cttcagctaa ttcttcgctt gttatccaat gattcttatt tcttaatact acaagaattt
    29461 ggagttgtcg tttatttaag tccatttgct tacacctcct ctttgttata aacatacagg
    29521 aaaaacctct caaatgacaa tagtcacttg cagaggttta aaaatcattt gaagaaagat
    29581 ctatattcaa ggtttttacc attagactgg ataatttgtt tataatattc aaaactatct
    29641 tttttcaatc tcctaaagct tccgctccca tcattttgtc tatccacata aataaagcca
    29701 tatcgcttcc tcatttcccc tgttcctgcc gatacgatat cgattggtcc ccaccataag
    29761 taaccaatga tttggcaacc atcttttatt gcctcagcaa tttcctgaag atgtattttt
    29821 agatactcac aacgttttgg atcgtggatt cgattcaatt catctggtgc ttcatctagt
    29881 ccaatgccat tttcaactat gaataatggt aattcataac ggtctgtcaa ttcattcatg
    29941 acataacgaa accctagagg atcaattggc caactccaag gttcgggaga tttttccttc
    30001 aaaaagggat tatcgaaacc atttgctccg ccagtatcta cgcgcatttc tgcttctttt
    30061 ttatagacag cgctacgata ataactaaat gcaatataat ctgccgtgtt agcttgcaag
    30121 cactgttcgt ctccatctaa cattactggt gcacagtctt cttccttcca tgtcctgtaa
    30181 atataagctg gataatgccc tcggcacatg acatcggaga aaaaccatga attgcgttta
    30241 aattgcagtg aaccaaatac attctctgga tcacagtcat atgcataagt cgctaatgca
    30301 gaaaagctac acataatacc catcttagca tttggatcga ttttctttaa taattgagta
    30361 gcacgggcgt tcgctagaaa catatgatga caggcttgat aaatcacttt ttttgttagt
    30421 gtagcaatgg gttcaagttt atctctagca gggaatgtaa ccaatccagc agctgcataa
    30481 ggcatacgaa aagcattatt gatctcatta aaggtcatcc aatatttcac tttcccttgg
    30541 tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt cgattaattt tcgattttgc
    30601 caccccccat actctcctac caatgctaag ggggtttcat aatgactaag agtaattact
    30661 ggctcgatat catatcgatt taatgtattg attagtctgt cataaaatac gagaccagcc
    30721 tcatttggct tctgttcgtc tcctcttgga aatattcgtg cccatgagat gcttgttcga
    30781 aaagctttta aacctaactc cttcagtaaa cgaatatcct cttcaaatcg atgataaaaa
    30841 tcaatcgctt catgagaaag ataatggagt gattcgtcta aagcaatctc ccaaatcttt
    30901 ttttcttcat tccactgaat actaggatgc tggttcatga tcccgataag cgtatctgtc
    30961 acattaggta atttgccgtc ttccaaccat gcaccttcac attgattagc agctactgct
    31021 ccgccccata aaaaattttc tggaaatatt tcctgatcca taactatctc tcctcgctat
    31081 caacaatttc ttacttgaag tatagctcga ttcatcaaaa caagatattc taactatttc
    31141 ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt ttaatattat atataacaaa
    31201 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    31261 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    31321 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    31381 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    31441 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    31501 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    31561 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    31621 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    31681 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    31741 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    31801 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    31861 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    31921 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    31981 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    32041 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    32101 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    32161 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    32221 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    32281 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    32341 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    32401 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    32461 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    32521 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    32581 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    32641 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    32701 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    32761 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    32821 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    32881 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    32941 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    33001 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    33061 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    33121 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33181 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33241 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33301 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33361 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33421 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33481 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33541 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33601 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33661 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33721 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33781 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33841 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    33901 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    33961 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34021 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34081 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34141 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34201 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34261 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34321 acacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34381 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34441 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34501 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34561 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aacataagta
    34621 aacaaaaaat tcggatttta agcaaaatcc gaacgttcca tctgattaaa tccctttatc
    34681 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34741 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgttc
    34801 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34861 atcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    34921 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    34981 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35041 aattgccttc agggtcagaa aaattgaaag tcaagatgcc attgttatct gtgatttctc
    35101 ctggttcttc catgttctcg tagatagttt caaattcatc cgtaaaaaat actaaagaag
    35161 gcggtggtcc taatacctct ggtgaaaact tctgcacaaa tacttttggg aatacgccta
    35221 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35281 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35341 tgacataaag catcacacgt gttttttgta tcatcatttg acccttcttt ctatttgatt
    35401 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35461 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35521 ttacaaaaaa aaaacggcaa tgaatgccgc tcttcttatt tcgattcttt tataattctg
    35581 cttctgattc atctttggtt agtgtgtgtc taacactagt tacgccaaat gtttgatcga
    35641 ttttttcctt caacgcttcg attgcctgtg cgccacgtgc cgcttgttca gcattgatgg
    35701 attcaatgac cagcaccgct gcttgtgttt cttccgtcag catcgttctt tgggcttcac
    35761 gccagttgag acagcgacag atcgctcctt gttcatcata gtagatgatt tcttccggaa
    35821 gtgccggagc atcggaatct gcacctaaag gcaggaatga ttcgccacct tgggcttttc
    35881 ctaaatgcag atttcctgcg atcttttcga tatcttctcc gccgcaaggt acgccaaaag
    35941 acaaggaaat actattatag atatctacaa gcggattgat tgggaaaaat tcccgttctt
    36001 gatttgcacg tttcagtaaa gcttcaatag aagaacgtgc acctttttta gtcttgaatg
    36061 tggtaaatgc ctgacgccac tgagcaatga cttcgttttg gctgaatgtt tcttctgtaa
    36121 ggtaatttct tgcttctttt gctgcctcat tcaataattg atgatacgtt tcttcacttt
    36181 cttttgttac gtggttatcg ataccattta cggataagat ttcgatggtt gcttcaggaa
    36241 atacttccca gaaattggta tccactatta cttttttcat ctgttcacac ctcttgatta
    36301 gaataattgc ttgatgaata gatagtctgt ttggggatca tctcccatat aaaaaacatg
    36361 ttcgctgtat ttgataaatc cttttttacg ataaaaagcg agcgcaacat cgttgtgttc
    36421 ccaaacaccg agccaaatgg ctgttttttg cttttctttt gctttttcaa tagctgtatc
    36481 tagtaaaaat gtaccgatac cttgatgttt aaattttttt cgtacataga ttcgctcgat
    36541 ttctaaaaaa tgatcagcca tttcctcaga ttgactcgtt cctgtattta gtttcaggta
    36601 acccgcaggc tcttgatcga tactcactag gaaaaaatcg gtttctttat cttttagctc
    36661 ttctgtaagt ttttctagtg cataagcttg tgtcaagtaa tcagctaaat gctccggtga
    36721 attatctttt ccaaatgtat ctgtaaatgt ttcgatacta atttgttgta gtaagtctac
    36781 atctgaaggc gtcatttttc gaatttcgta tttattcacg gcttcttcct ttcaatagat
    36841 ccttttattg ccttttttga caaaattcca atcttcgcta acattcttgc atactttatt
    36901 taaaagatat tccaattgtt ttgcttcttc gtcagaaaga ccatttaatg cgactgcatt
    36961 ggagtagttg ttttctcgaa tgatgaaagg atagatttct gctccttttt tagtcggata
    37021 gagtttcttg atttttttat tttctttatc ttccaaacgt tcgatcatcc cattggattc
    37081 taatttcttg attgctcggg cagtcgttgt acgatctact ttgatcattt ctgctagttt
    37141 ttcttggatg attccaggat tttcgcaaat acgtactaaa taaagatact gtcctcttgt
    37201 taaatcgact tctttgaatt cgatattgct aatagaatct agcgctcggg caatcattcc
    37261 gatgtctcga aggatttcag ccatttcatc ccacctttat tttgtttgta agactagttc
    37321 acctcttttt gttgcaaatg caactatttt caataaaaaa atcaagttct tttttaataa
    37381 ctagtaaagt atattctttt cttcttttac gaaaaagctc aatcacaata gatctggttt
    37441 ttgttttcta aaataagtaa ctgtttcttc gttgttcgct tttctgatag ctttggcaac
    37501 tcttctatac taaaaaatcc catgttggaa gtttcattat tctcaataaa cttggcctct
    37561 tcaccgtgga tcgcacaagc aaaaacaagt ttataatact gaaaaagctg tggaatatct
    37621 ttttgtttat tcgtatcaaa aactgctcgc aactgagtag tttctactgt taatcctgtt
    37681 tcttcataga cttctttacg cacattttct tcaggagaca atcctatttc agcaaatcca
    37741 ccaggaagtg accattcttt cgtccgttta tcttcgatta gtaggacctt gccatctttt
    37801 ttgatatagg cacgaacatc tacttttggt gttggatgtc cttctccaat cgttgtgagc
    37861 gcttttattt cttcgaaagt ttcttttcct atttcactga tcagttccaa tgaaatcgtc
    37921 cggatctcct caaaacgctc tttatcaaat acatcttttc cataatatag accagcttga
    37981 gaaagtgcga gtagtctttt gtattgagcc aacttcttgt tcattctctt cccttctttt
    38041 ttagcaaatg tatgactatg taatcaagta tagaagactg gtttaattac ggcaaataaa
    38101 tataatgaaa tctgcaataa ataaaagaat gttaaaaaac tcgagaacaa aagacattcg
    38161 acttctctgg ggctgtgaat aaatggcgtt caaaagcttg catctgcggt aataagcccc
    38221 accaaacgaa aaatatgacg agctattctt gtctggctgt tcttattaat tgaggcagga
    38281 cagctttttc acaacctccc cactaaacgg tgttctccta ccaactcctc ggtcacaagt
    38341 cacaatattc atcaaacatg agaagctgtt tttttgaaat tgctccttgt gactcggagc
    38401 tgaacgacag gctcacgacc tctcggtaaa cagcgttcaa aagccaattt ttcgatatta
    38461 agccatttct ttgttactta ttgtcattga tttctttttc taatttatcc aatagtggag
    38521 tgtaatccac ttgagtatag tctgttacat caaattcata tagtgttcct aactggaatt
    38581 tgtcatattt tcggtcggcg atgatctttt tgaatgcttc tttcgtaata tgattggtcc
    38641 ctttcgttcg atcttccaat acgtcaccat agtgataatg atccatgata tagctcaaat
    38701 gacgatctga ttcacgatca cgtttgtacc gacgttgcca taacacttca aagtctgctg
    38761 ttaggcgaat cgtgatcacc tgatatccgt atctatctgc taattcttgg agtcgtgctt
    38821 tttgcttatc actaaaagga tactcggaca caatcacttt ctttcctgct tccatataca
    38881 aatcaagtgc tttgtaatag aaaggataaa ctttcgtcct ttcttgttcc gcccgttctt
    38941 ccaaattgtt aaacccgatg gattctgcat agtattcctt gatttcatcg agcgtgatgg
    39001 catacatatc tggaaatcgt tcccgtagtt tattcataag atatgttttt cctgtaccag
    39061 gactaccagc cagaagaatg aaatactttt tcatctcatt tatttcctaa gttaattgga
    39121 ctttccaagt tcataaacag tccaatctta tgcttgacaa tttcttttac tgcttcattt
    39181 gctggaatca cattatggcg aagagacttg ttgatttctg tttcactaaa ggtttcttta
    39241 gctactttcg tccagttgac taaaagctcc gttccaacat tgaatttgcg gatcccgtta
    39301 ttgatcaatt caggataatc ttcttctttc acacctgttc ctccatggat aactaggggg
    39361 acatcaacta ctgcatcgat ttctttcagc aagggaatgt ttacttctgt ttttgatttg
    39421 aattgtccat gattcgtacc gatagcaata gctaatgcat ccacgcctgt agcttctaca
    39481 aatttcactg cgtcttccgg tcttgtatat accttgtcat tttcagcgac atggattcct
    39541 tcttctgtac cgccgattgt tccaagttct ccttctacgg aaacaccgtg cgcatgggca
    39601 tattctacta ctgcttttgt cttcaggata ttttctttga aaggcagatg ggacccatca
    39661 tacatgacag aagtatatcc gctgtcgatt gcttctttga tatcatcaaa gtctcgggcg
    39721 tggtctaagt gcaagactgc atcaaccatt tcttcttctg caatagctgt cattacattg
    39781 actaacacat gcatgccgat atatttagcg gtatcgacac ttgtttgaat aatgatcggc
    39841 gaacccattt cttttgctgc gcctagcata gcaggaagca tttctaggtt atgcgtatta
    39901 aaagctccaa ctgtaaaatt caactcttct gctgtttttg ttacttcttt caatgttgta
    39961 tacatctttt attgccacct tatcttttta tttgtttcct ttgatcgtta cttgttttgc
    40021 aatttgtcgc atctcatcca ttttgcccat gtagtaatcg atcccttgat catcaccagc
    40081 acgagcagca tctgcacgct tctggttata gaggctcatc agttttttat acccatctcc
    40141 gatcgtctgt ttattttcca aactcttttc taaatattgg atggcttgat cgttcacata
    40201 caattcagaa aatttaagtc caacttgttc ataaagtttc gctaatttat gcttatcttg
    40261 ggtagtggaa atctcttgtt tcaattgtgt aatctctgat tgaatctttt cggtcgcttc
    40321 tggcgatagg gctttttctt cttttgccag aacttttttc ggttcttttt cttttttctt
    40381 aaaaaatcca aacatcattt tccctacttt caattaaaat gctttcaaaa ctgggcttaa
    40441 gatccaggca tataagagtg ctgcggctac tgtatctaca tcggttgctg tagcattgtt
    40501 gaatcccatc gaattaaaga tcgttactag caaagcgggc aataatgtaa taaagaaacc
    40561 atgagcaata ccgccaatga tcgctcctcg gcgtccgcca actgcgttgc caaatatccc
    40621 tgctgtccct ccggcaaaga aattggttag cataccaggt aaaatcatcg ctaagccgaa
    40681 catgggcaaa gtaaacatgg caataatcgt accaatcgtt gtcgtaatga aacctaaaat
    40741 gacagcattt gggctataag gaaagaaaac tggacaatct aaggcaggga tcgcatcagg
    40801 aactaatttc atagctatcc ctcgaaatgc cggcacgatt tcacctaaca gtagacgtac
    40861 accagctagc aatacataca cacccactac gaattggatg gcttgtaaaa aggcaaacat
    40921 gatatagttt tgatctcctg ataattcaga agcgaatggt tcgcctgcaa ataaaacagt
    40981 gatgatatat agcgggacca tcacgaccat gacagacaaa taagtatctt gcagaaattc
    41041 gaaatgtgtc ggcaacttga tatcttcgat cgacttttta ttttcgcctt tctcaccaaa
    41101 aagcttagca acaccggctt caaataagta cccgatcgta caaaaatgac ctaaagcaat
    41161 atcatttgat ccagtgattt tacgaatgat cggttgcgct actgccggca tagcaatggc
    41221 gaaacaagct ccgataaaac caccgaccaa gatcaatacg atccctcgca gaccggcaaa
    41281 atagccaaag actgtcgtca tcgttgccat ccataaaatt gcttgaccag ttaaaaaaat
    41341 gtacttccat tttgtgaaac gagccaaaat aatattgaat acaaaaatgg ctaagaacgt
    41401 cagtgcaata tctctaccta aacccaaatc attcattgct tgaccattga tagattcaat
    41461 agaagggatg atcccttgca tatgaaatcc ttcagtaaag attttcccga aataagttaa
    41521 acttccaacg atgatgctcg aaccggcact caacacttga aatcctagga gtgtctttaa
    41581 cgtgccagag atgacttgtc ctgctgattt cttctgcagt agtagaccca acatagcaat
    41641 caatgcaatg gtgatcgatg cctgagtcaa aatattttcg atgataaaat tgataacgcc
    41701 catgtt
//
LOCUS       DAYYGS010000015        41432 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000015 DAYYGS010000000
VERSION     DAYYGS010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.015"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..499
                     /locus_tag="ACHA44_000752"
     CDS             29..499
                     /locus_tag="ACHA44_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HFM8904066.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            1128..2231
                     /locus_tag="ACHA44_000753"
     CDS             1128..2231
                     /locus_tag="ACHA44_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HFM8904067.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            2515..3195
                     /locus_tag="ACHA44_000754"
     CDS             2515..3195
                     /locus_tag="ACHA44_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8904068.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            3285..4319
                     /locus_tag="ACHA44_000755"
     CDS             3285..4319
                     /locus_tag="ACHA44_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8904069.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            4333..4668
                     /locus_tag="ACHA44_000756"
     CDS             4333..4668
                     /locus_tag="ACHA44_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904070.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            4652..7132
                     /locus_tag="ACHA44_000757"
     CDS             4652..7132
                     /locus_tag="ACHA44_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904071.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            7188..8747
                     /locus_tag="ACHA44_000758"
     CDS             7188..8747
                     /locus_tag="ACHA44_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904072.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            8744..10108
                     /locus_tag="ACHA44_000759"
     CDS             8744..10108
                     /locus_tag="ACHA44_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8904073.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            10279..11349
                     /locus_tag="ACHA44_000760"
     CDS             10279..11349
                     /locus_tag="ACHA44_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904074.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            11364..12032
                     /locus_tag="ACHA44_000761"
     CDS             11364..12032
                     /locus_tag="ACHA44_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904075.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            12052..12630
                     /locus_tag="ACHA44_000762"
     CDS             12052..12630
                     /locus_tag="ACHA44_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904076.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            13033..13944
                     /locus_tag="ACHA44_000763"
     CDS             13033..13944
                     /locus_tag="ACHA44_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8904077.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(14200..15117)
                     /gene="rnhC"
                     /locus_tag="ACHA44_000764"
     CDS             complement(14200..15117)
                     /gene="rnhC"
                     /locus_tag="ACHA44_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HFM8904078.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            15319..15756
                     /gene="zapA"
                     /locus_tag="ACHA44_000765"
     CDS             15319..15756
                     /gene="zapA"
                     /locus_tag="ACHA44_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFM8904079.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            15814..16361
                     /locus_tag="ACHA44_000766"
                     /pseudo
     CDS             15814..16361
                     /locus_tag="ACHA44_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            16476..18836
                     /locus_tag="ACHA44_000767"
     CDS             16476..18836
                     /locus_tag="ACHA44_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HFM8904080.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            18923..19237
                     /gene="trxA"
                     /locus_tag="ACHA44_000768"
     CDS             18923..19237
                     /gene="trxA"
                     /locus_tag="ACHA44_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HFM8904081.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            19419..21197
                     /gene="uvrC"
                     /locus_tag="ACHA44_000769"
     CDS             19419..21197
                     /gene="uvrC"
                     /locus_tag="ACHA44_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFM8904082.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            21342..22241
                     /locus_tag="ACHA44_000770"
     CDS             21342..22241
                     /locus_tag="ACHA44_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904083.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            22234..23496
                     /locus_tag="ACHA44_000771"
     CDS             22234..23496
                     /locus_tag="ACHA44_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904084.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            23659..25557
                     /locus_tag="ACHA44_000772"
     CDS             23659..25557
                     /locus_tag="ACHA44_000772"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HFM8904085.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(25856..26575)
                     /locus_tag="ACHA44_000773"
     CDS             complement(25856..26575)
                     /locus_tag="ACHA44_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HFM8904086.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     misc_feature    26765..26812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF03071"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="sodium ion sensor (DUF1646 type); this
                     cis-regulatory element may regulate processes involved in
                     with the transportation of sodium ions; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF03071"
     gene            26912..27244
                     /locus_tag="ACHA44_000774"
     CDS             26912..27244
                     /locus_tag="ACHA44_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904087.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            27231..29234
                     /locus_tag="ACHA44_000775"
     CDS             27231..29234
                     /locus_tag="ACHA44_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HFM8904088.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            29253..29723
                     /locus_tag="ACHA44_000776"
     CDS             29253..29723
                     /locus_tag="ACHA44_000776"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HFM8904089.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            29744..30328
                     /locus_tag="ACHA44_000777"
     CDS             29744..30328
                     /locus_tag="ACHA44_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HFM8904090.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            30343..31329
                     /locus_tag="ACHA44_000778"
     CDS             30343..31329
                     /locus_tag="ACHA44_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HFM8904091.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            31319..31630
                     /locus_tag="ACHA44_000779"
     CDS             31319..31630
                     /locus_tag="ACHA44_000779"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HFM8904092.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            31652..33433
                     /locus_tag="ACHA44_000780"
     CDS             31652..33433
                     /locus_tag="ACHA44_000780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HFM8904093.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            33426..34802
                     /locus_tag="ACHA44_000781"
     CDS             33426..34802
                     /locus_tag="ACHA44_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HFM8904094.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            34809..35444
                     /locus_tag="ACHA44_000782"
     CDS             34809..35444
                     /locus_tag="ACHA44_000782"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HFM8904095.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            35474..36829
                     /locus_tag="ACHA44_000783"
     CDS             35474..36829
                     /locus_tag="ACHA44_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFM8904096.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(37021..38415)
                     /locus_tag="ACHA44_000784"
     CDS             complement(37021..38415)
                     /locus_tag="ACHA44_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFM8904097.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            38507..39478
                     /locus_tag="ACHA44_000785"
     CDS             38507..39478
                     /locus_tag="ACHA44_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HFM8904098.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            39475..40257
                     /locus_tag="ACHA44_000786"
     CDS             39475..40257
                     /locus_tag="ACHA44_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HFM8904099.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(40426..40797)
                     /locus_tag="ACHA44_000787"
     CDS             complement(40426..40797)
                     /locus_tag="ACHA44_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8904100.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            40931..41374
                     /locus_tag="ACHA44_000788"
     CDS             40931..41374
                     /locus_tag="ACHA44_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8904101.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
BASE COUNT        13712 a         7043 c         8825 g        11852 t
ORIGIN      
        1 agtagaaaat taagaggagg aagagaaaat gaaagaagtt caattgatta gaaaaagcga
       61 actatcagaa ggcggttgca atgcatgtgg cgtagtagaa gcgacaagct atacattaaa
      121 attagattcc aatcaagcaa taatctctga actgacagta ggcggtcttg tggacagtct
      181 ggcgcttgct gaaggtttta ctggagaaga tatttatgag atgttttcag aagtccgtca
      241 gttaaaaaaa ggagaaaaat gcatagaggt tcatcatgag agcccgaatg tgaggttcaa
      301 acgtggtgat aatgagatga tcttcaagaa tcatgtgtct gatcacactg aactatatga
      361 aatcgtgaat caaatactgt ctgggatttt tgaattagga ccttatgagt tcaaggaaga
      421 aaacggtaat ccaaaattaa atgaagaatg gcaagaaacg attgaaacac aaagaaacaa
      481 tccacactta tttcaatagt cttccacagt atttcgtaaa aatgttgatg aaaaagaaac
      541 accgtgaatc agaggtcgtg aaaaacctgc cctgcatcaa gtaataagaa cagccaaaca
      601 aaaatagctc ctcctatttt tcgtttggtt gggcttatta ccgcagatgc aagtttttga
      661 acaccgttta aggaagagac tgtgaagtca acgttctgac ctgagtatta gagagacaaa
      721 tggcaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg tcagctgatt
      781 gaacaccgtt ttatcgagag gttgtgaaaa agtcgcttag tttcgagtag taagaaaaat
      841 tttgtgaaaa tggctttcca catttttgca taaatttgac ttaataccga gaaaatggct
      901 tttgaacacc gtttattcaa aaaaagggac tttgacacta cttttgtcaa agtcccatcg
      961 gttttttatc tatttttatt acaatcattt cttccagagt agtgacatct ggaatggctg
     1021 aaatgtaaac agattatttg cctgtatcaa tgtcgaagag taatataaaa agtgtaaacg
     1081 ctatcatatt gttcatcatt caaattttat agagagaagg cattgagatg gaaaaaacat
     1141 atcaagcagg tacaaatgaa ggaatagtag attttatcaa tatggaagat ttagaaatcg
     1201 cggcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt gctggcgact
     1261 tatttactta tcaagaaaat gaacgggcat tcaatcatcg tttgattatt cctcatgtat
     1321 taagagatgt agaattaccc gatacaacta cacattttga cgaagaaatg ctgactgcac
     1381 caatcatcat ggcaccagtt gctgctcatg gattagctca tgtgaaggcg gagaaagctt
     1441 ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac gcttcttgta
     1501 ctttagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt caattttata
     1561 tgagcaaaga taatggaatc aatttggata ttcttgaagt ggccaagcgt aatggagcaa
     1621 aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa
     1681 acgggtttac cttcccgctg ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc
     1741 aaacgatgga tgctgtatat aaatcttcca aacaaaaact tagtccaaaa gatgtagaat
     1801 ttatcgctgc tcattctgat ttgccagtct acgtgaaagg tgttcaatca gaagaagatg
     1861 tctatcgatc attagaatct ggtgctggtg gaatttgggt atctaaccat ggtggccgtc
     1921 aattagatgg ggggcctgct gcgtttgatt cgttgcaata tgtggcagaa gcagtagata
     1981 aacgtgtacc aatcgtgttt gacagcggtg tacgacgtgg acaacatgtt ttcaaagcaa
     2041 tcgcatcagg tgccgatctt gttgctattg gcagacccgt tatttatgga ttgtctttag
     2101 gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg gaaatggtca
     2161 tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg cgtgagaatc
     2221 gttttattta agaaataaat gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc
     2281 tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa ataaaattga
     2341 aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt
     2401 tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc atgaggattt
     2461 tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt tattatgaaa
     2521 aagaacatga ttgggacatt attactttca acacttcttc taggcggctt ggcaacacca
     2581 gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga accaacaaat
     2641 accgttgagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc agatccggaa
     2701 aatccagcaa ctggccagac aggttcttta acattggatg tagttcctga attgccattt
     2761 ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat
     2821 acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca agcacaaggg
     2881 tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct
     2941 gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc cactggacca
     3001 actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat atttacagca
     3061 gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa tatcacactg
     3121 aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa cctcgtggct
     3181 ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat actataggtg
     3241 aaggattttt taccgcagtt gttgttgaaa ggatgtggtg gacaatgaat caaagtaaac
     3301 gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg tcaatcagcc
     3361 aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag aaccaaatag
     3421 ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag acactagaag
     3481 taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc aatccggcgg
     3541 taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta gatgagacat
     3601 tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac
     3661 atgcagaaac aactatacct atagaagtga aaatcccagc aaaaagcttt aagggacgtg
     3721 tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat gctacagaag
     3781 gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa gaaagtcaag
     3841 agacgataga accggatctg aaacttttat caggagattt ggacgaggtc aacgcaaagc
     3901 caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat ttgatattta
     3961 cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat gaatttttag
     4021 ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa gcaaaagccg
     4081 gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg cgatttacac
     4141 aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag
     4201 tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt ctagtgttat
     4261 ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa acaacatgat
     4321 gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag tttgctgttc
     4381 ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat tacattttat
     4441 gatctaagtc cagttccgca tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca
     4501 cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac gaatgaagaa
     4561 aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc gtggttgttg
     4621 ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat tttcattttt
     4681 tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt caccttcggc
     4741 agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt atgaaaatat
     4801 cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc aactaactgg
     4861 ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa atgatccttg
     4921 gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa atgtacttga
     4981 tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt tgccggaaca
     5041 tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact tgtatattgc
     5101 cggtgccccc atcaaggtta ctagcggtag tttattatca gcaggaagcc ataccttgcg
     5161 ttttgggatg gggtcgtggg atacaaatac tctattggca gatattattg ctattattac
     5221 gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag ctaatgtcgc
     5281 gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt tgaccaaggg
     5341 taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt atgattcgaa
     5401 taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag gtagatttta
     5461 tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg caagtgcaga
     5521 gataaatacg tggaatagtt acatctcaac aagtgattca acaggaaaat ataatacatt
     5581 tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct ctaccaatag
     5641 tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat ttagtcgctt
     5701 tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa tactgactca
     5761 tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca gtgggaaaga
     5821 tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt acaagctaga
     5881 cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc tcctttcttc
     5941 tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca
     6001 ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag atcaactttt
     6061 ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac tacctgcatt
     6121 ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta ctaacatgta
     6181 tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc ttcctcaaat
     6241 aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca
     6301 acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag aaaatgtgac
     6361 attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga tctcacttcc
     6421 tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta tcgcagatac
     6481 cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag gagataccat
     6541 tcatcttact tacaactatc aattaaatac tgtagatact tctgtacaat ccgtcagcat
     6601 actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg ctaagcttcc
     6661 taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag aattaacatt
     6721 agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt tgaagacaag
     6781 ttattatgaa gcaaaaggcg atttttcttt tgatgtgcgg gatactcggc ttccaagcac
     6841 gtctccttgg caactgacag ggacattgac ttctttattt aagaatgatc aaggtcaaga
     6901 attaagcgga acaaaattat acttcaatca ttctggatca aaacaattga ttcagcaagg
     6961 tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc tcgttgactt
     7021 tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc aacctggcgc
     7081 aacgtatcaa ggcatggtca catgggaact gacagctggg ccaaccagct aagtgattag
     7141 aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg agaaggaaac
     7201 ttctgctgtt aaaatcagaa atgcttggag gagtggactt ggaacttttc agtttgcttg
     7261 aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa aaaggagaga
     7321 tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg aaagaagatc
     7381 tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt atccaagaag
     7441 ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat tatcattgct
     7501 acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat ggctcatttg
     7561 atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct cgcagagtga
     7621 aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg cggctgaccg
     7681 gtccagaaag tcaaatttgt tatttttatt tccaattatt ttggtttgga cgtccttatc
     7741 ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat ctgctgcaaa
     7801 aagagtttag ttttactatt acagaagaag gacgattgaa atttgaaata tggctttcca
     7861 ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc aatcttcctg
     7921 ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg acccgttact
     7981 tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat cttttcatga
     8041 tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg ataagcgaaa
     8101 aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg atagaagaat
     8161 atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc
     8221 aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc caagaaggat
     8281 taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt gaaagattgg
     8341 tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat gcccgctttg
     8401 aattatttgc tcgctatgca tctattttgc ggacgctgat caagcagtta gaattacgtc
     8461 tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca
     8521 tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag aaagaaaagc
     8581 agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag tccgctaaag
     8641 tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac caatttttaa
     8701 aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat
     8761 tggtgcttcc catgcaggca tcacagctgc cctaaatcta agaaaacttc aaccgaaagc
     8821 agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt caaatggaat
     8881 caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata acatgcggac
     8941 tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac ttgatccaga
     9001 caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa cctatgataa
     9061 attaatcgta gcaacaggtt cctctcctgt caccttgtat aaacaaattg aagctgaaaa
     9121 tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt taaaagaagc
     9181 aaaagaagta gtgatcttcg gtgctggtta tattggattg gaattagcag atgctttgag
     9241 aaataaagga tacattattc atctagttga ttatatgccg aatgttttat caagatattt
     9301 cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa tcaattttta
     9361 tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat ccgtacaact
     9421 tctatctcaa gcaataaagg cagatatggt cattttttct gcccaaactc gtcctaatac
     9481 gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag tcaatgaata
     9541 tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag tcagttttga
     9601 caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata tggcaagagc
     9661 agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa agataacagc
     9721 agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg aagcgccatt
     9781 cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac aatacgatga
     9841 agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag aaataattgg
     9901 cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg cggatatcgt
     9961 gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc tgtctgaaca
    10021 cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta agagagcaaa
    10081 tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg tgtggggata
    10141 taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg gaagaatttt
    10201 ttgagcataa tattcgccaa aaatttatca agtgataaaa ttgttgtata aaatgattat
    10261 ttcaggagga ggagttaaat gtttttagca ttaaatgaaa ttatgcattc aaagttacgt
    10321 tatgcgttag ttgcaggggt gatgtttttg atcgcctacc tggtattttt tttgactggg
    10381 ttagcttatg gcttagctca agataatcga acagcagtcg acaagtggga ggcagattcg
    10441 attgtgcttt cgaaagatgc aaattcaaat ctagggatgt ccatgattac taagaagata
    10501 gcagaagaag tcgaaggtgg gaaagtggcg tatcttgccc agacacctgg agtcgtgact
    10561 agtaaagaca gtacagaaga aggaaaaatc aatgttagct tcttcggtat cgataaaaat
    10621 caattcatca tgcccaatct tgttgaagga aaagcttttg ataacgatga tgaggcagta
    10681 ggcgatatta gtttgaaaga agaatatggt ctagccgtag gagatacagt taagttatct
    10741 ggtagtgaca agacgtttaa actaactggc tttacggatc atgccaaatt caatgtctca
    10801 ccagttctct atacaaccat caatgcttac caagagatcc gcttcgaaaa agaagataca
    10861 agtgaaaatg cacggattaa cgcaatagtt gtcagaggta aaatcagtaa tttacctgaa
    10921 gatttagaac agatcaaaat ttctaaattc atcaatgaat tacctggata taatgcgcaa
    10981 gtattgactt ttggatttat gatcggattt ttgatcgtta ttgcggctat tgtgatcggg
    11041 attttcattt atgtactgac gatgcaaaag attaatatct ttggtgtaat gaaagcacaa
    11101 gggatcacag gaggatttat tgcgcgttct gtagtggcac aaacattcat tttatcattt
    11161 gttggtattc ttcttggtct attaggaaca gtgggaacat cccttgttct acctgatgcg
    11221 gttccattcc aaagcaattg gctgtttttt ggcgtcatta gcttgttgat gctggtagta
    11281 gctgtgttag gcgcactatt ttctgttcgg acaatcgtaa aaatcgatcc gttgaaggca
    11341 attggataag aaggaggaag agcatgaaag caatagaatt taaagaagta gaaaaaagtt
    11401 tccaagatgg cgaccaagta gttaaagcat tgaaagaaac aaccatgagt atcgataaag
    11461 gggaatttgt tgcaatcatc ggtccttctg gttcaggtaa aagtactttt ttgactttgg
    11521 caggcggatt gcagacgcct agcagtggct ctgtatttat caatgatcaa gaattcagta
    11581 atcaaaaaga aagtgaacgc gttaaactac gttttaaaga aattggcttt attttgcaag
    11641 cttccaatct ggttcctttt ctgacagtaa aaaagcagtt attattagta gataagatca
    11701 aacatgaaca tcgtcaaaaa gaagcaaaag aattatttga gcagcttggc gtggataagt
    11761 tgatcaacaa gtatccagaa gaactatctg gtggagaacg gcaaagggtc gcaatcgctc
    11821 gtgcattgta caatgatccg tccattattc tagcagatga accaactgct agtttggatt
    11881 cagaaaaggc acgagaagta gtcgatatct tggcaaaaga atcaaaagac aaaaataaag
    11941 caacgatcat ggttactcac gatacacggc tgattgacaa atgcgataga atatttatga
    12001 tggaagatgg tgtattgaaa gaaaaagaat aaccaaaaag caggtggaaa aatggtccat
    12061 cgatacaaca aagatacaca gcaaaaaatt ttgaaagaag cgactgactt gtttatgtcc
    12121 aagggatatc tcggcacttc aacaagagag atcgcacaaa aagttgggat cacccaaccc
    12181 aatctctatc attatttcgg tgataaagaa aaactatata cggcagtttt agaaaaccac
    12241 ttaaaagatg ttggaaaagc tttgagagaa atcgttcaaa caagcgaaac gggatttcaa
    12301 gtaaccttga caaaaatggc gcagtttttg attgagacac atctggtcga tttgtttctg
    12361 atgcttcacg atcttgaaag caatctttca aaggaaacaa gagatcatct tttctttcta
    12421 tggaagaaaa attatcgtga accttttgaa gaaatctttt cagcaaacca atctgtgcta
    12481 agaaatggga tttcaaaaga aatcgcagca aggcattttt tcttagtgtt agcaccttac
    12541 atcactcggt cgaaaagacc tgtggaagaa ccgctaaatg tggcgcaatt gatcgattta
    12601 tatttgaatg gtgtattagg agaagcgtaa agctgttata tagggagaaa aatagttagg
    12661 tgaagaagca agatatgacg aagatcgtta tatcttgttt tttttatata ggtagttttt
    12721 cataaataca aatttgttat tctctcatga ttatcagggt atatttttat ttattatgtc
    12781 ggtgttccag gaagtgtttt aatttaataa cgaagaaatt aattgtttgt agaatgaagg
    12841 aaactattta ttgggtagat gaatcaccac gaatagtcaa aaggagattt caggatatgc
    12901 caaaaatgaa aaaaatcatt ttagcagtaa tgttattttc tacggttata tcttttacag
    12961 cccctttttt gcgtacttaa gctgatttta agttttgacc cttagtctgg atctacgagg
    13021 tgagagtcaa gaatgaaaaa aataattgga gcttttgtat ttgcaatcat tatcattatt
    13081 atctgttttc aaatagatgt ttttggtcaa aaaaagatag ttggacaaac ggtcagcagt
    13141 acgaacagta cggttacaaa gacaaaggaa tcatttgatg atacagaaca agagacatta
    13201 atcaaaatcg gaaaaaatga ccgtacacta tatacagatg actcgctaac taccccactt
    13261 gcaactataa acggcggaga gctggcggag tttctttctg aaacaaaaaa tgcttatcag
    13321 atcaagacaa atgatggcta tactggatat ttagccctcg aagatggaac caaagtcaca
    13381 aagaatatac aaacaaaacc ggaagagcta tctgatgcag tgattgtatt agatcctggg
    13441 catggcggaa atgatactgg ggcattaagc aatgatgaac aaacagaaga aaaagatata
    13501 acattaagta cagccataaa agtaaaaaag gcgctagagg atgctggagc aacggtttat
    13561 ttgacccata ctacagatga attagtacag cttggagata tatgcgacta cagtgaagaa
    13621 aaaaaggcag atgtctttat cagtttacac gcagactcta cggaatatgc gaatgaagca
    13681 actggaatca cgacatatta ttactatgga caagaggaaa cattagcaca gacaattgct
    13741 gactccttca ctgatctgcc tctggattca agaggaatct ccactgggaa ctatcaagtt
    13801 cttcgagaaa atcttcaacc ttctatttta atcgagatgg gttatatgaa taatgatagt
    13861 gatttaaagg aattagtaac agacagttat cagcagcaga ttgctgcaag tttgacacaa
    13921 gcattgacca cttattttca gtaataaata agcccagcca aacaaaaata gctcctcata
    13981 tttttcgttt ggccgggctt attatcgcag atgcaagctt ttgaacaccg tttaaggaag
    14041 aggttgtgag cctgccgttt aactccgcgt cacaaggagc aatttcaaaa aacagcttct
    14101 catgtttgat gactattgtg acttgtgacc gaggagttgg catgagaaca ccgtttattc
    14161 ggaacaaggg actgtgacat gacttttgtc acagttccat tattttttta gcagcttcaa
    14221 agctttttct gtattggcaa aatgaagttt ggcatatttt tcaagcatag gcatgccgcc
    14281 tttctccaat agttttgccg ctacttggtc agatttcgtt ccagctcctg atggaagggt
    14341 gattccagct tcagctgatg ctttgtgtaa ctcttccaaa aagctggcac gactgatgat
    14401 agaagcagca gcaacagcta aatgatattg ttctccttta gtaacgaagt acagtttttc
    14461 ttgtaattga tttttttcta aacgcgcata ttttttaaaa tttgcttccg gcgtaaactg
    14521 atcgatcaaa atcgcttctg gtttttctgg ctcgattttt tttagcagca aatggatcgc
    14581 ttgattgtgc aaagcgactt tcatccgaac ggcattgtat tttggttgga tctcattgta
    14641 tttttctgga gataagacaa gcagtttata ggggatggtt ttttttaaga tgtcagcaag
    14701 ctggatgatt tggctatctt ttaactcttt tgaatctcta acaccaagtt tacgtaattc
    14761 tgcaagctga tcacgtttca cataagcagc acatactgtt agcggaccaa aatagctgcc
    14821 atttccgacc tcatcactac ctaaaacgga taattgactg atattttttg gcaaagaaga
    14881 ggctgttttt gctggcttgg cttgtttatc ctgtgcctcc ccccatttat cagcttctat
    14941 tgctgcattc gtaccttgaa acatcacttt tccggatgta taagcggtaa tggtcgtccc
    15001 gtcttttttt gcagcaaagc tagtataagg aatctgttta tttaacaaat aagagcgata
    15061 gtatgctttc atttgactaa gttggtcgtt tgttgctttg ataatgacgt tgctcataaa
    15121 attctcctcg tttttataca taaaactata ccgcaactca cgacttttcg ttttctaaga
    15181 gattgcgaga catagcattt ttttaaagtt tatcatataa tagttaagta tgtgaaaaat
    15241 atttgtggag aacggtgaaa gaaccaactt catggtacac taaataaaac tttcatcaaa
    15301 taggaggaaa tgattcgtat ggcgcacgaa aaaacacgct acaaagcagt gattgccaac
    15361 caaacatata ctattatcgg acgggaaacc aagcatcata tggatatcgt aacgaagctg
    15421 atcaatgaac agttagcaga attgaaacaa ttatcaccac aaatggataa tgaacaagca
    15481 gcaatcttga tggctgtcaa tgcgctttcg gatcaattga agaaacaaga aagaatcttg
    15541 gagctggaag aagaaacagc agaattaaag aaaaaaatga tcaaatttac agagcttgaa
    15601 aatcgtgtga aacgcattga agcaatcgag aacgaagctc gggaagtatt gaaagaaaat
    15661 ggtcaagcag atcataccat taaaaatcat gtggaagcac aacagatttt aaacgagaaa
    15721 cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa aataagaaaa
    15781 atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat tttattttta
    15841 ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat catattcgcg
    15901 attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt agcaagtcat
    15961 ttggaattat acattccata cccagctgtc accccaacat caaagttagc attttttgat
    16021 caggtatttg cttttcacct agatgaagct ttttatgcag gaactgcttt tctgattatt
    16081 ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg attgacgtat
    16141 gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact aatcatggct
    16201 tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga tgtggtccaa
    16261 aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc atttcttacc
    16321 aacaagatgt atgagctttg gtaacgaata ttttgaatta aggaaaatag tgctggaaaa
    16381 tatttcgttt tttgtacaga gtagtttatt taagaaggct gcgtagcgta tgtccggcac
    16441 agctcttttt tcatagtgaa agataaggtg ataaaatgaa taaccgaatc cttgaaacat
    16501 tggaatttga aaaagtcaaa cagatggtca gacaatttgt agtgactgca caaggaaaag
    16561 aagaactggc agaattggta ccagtgagtg agaaacaaac gatcacaaac tggcttcaag
    16621 aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata cctaaacttg
    16681 aaaatatccg tccccatatg aaacggatcg agatcggagc agacttgaat ggagtggagc
    16741 ttgcacaagt ttccagagta ttgtctacga caagcgaatt gaatcgtttc attgatgatc
    16801 tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag ttagtcacga
    16861 tccctgtttt gagccgccgt ttaaaggaag caatcgatga agacggacgc gtgactgatg
    16921 acgcttctcc tgagttaaag agcataagac agaacattcg gcgaagtgaa caagctgttc
    16981 gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa gtatttaagt gatgcgatca
    17041 tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca agaataccgc ggtgtatttg
    17101 gcggtgtagt ccacgatcaa agtgcttcag gacagacttt atttattgaa ccaaaacaag
    17161 tggtggattt gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat gagattcaac
    17221 gtattttaag tgaactttct gctgaacttg ttccccatcg tcaagaaatc atccacaatg
    17281 cttatgtgat tggtaaaatg gatctgatga atgcaaaagc acgatttggt aaagaagtaa
    17341 aagccatcgt gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa gcaagacatc
    17401 cactgatcga tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa gactatcaag
    17461 ccatcgtcat tacaggacct aacacaggtg ggaaaacgat cacgttgaag actttaggtc
    17521 tgttacaact gatggggcaa gcaggattac cgattcctgc aggtgaagaa agtcaaatcg
    17581 gtatttttga ggaagtattt gcggatatag gagacgaaca gtcgattgag caaagtctat
    17641 cgaccttttc ttctcatatg acgaacactg tagatatttt atcaaaagtc aatgaaaaaa
    17701 gtctggtatt atttgacgag ctcggtgcag gaacagatcc gcaagaaggt gctgctctag
    17761 caatcgcaat cttagatgat cttggtaaaa agtctgctta tgtgatggca acaacgcatt
    17821 atcctgaatt gaaagtttat ggatacaatc gggcaaatac gattaatgcc agcatggagt
    17881 tcgatgtgga tacactaagt ccaacgtatc gtttgctgat tggcgttcct ggacgaagca
    17941 atgcgtttga aatctctagc cgattaggtc ttgatacaga agttattgat gaagcgaagc
    18001 agttgatgaa tgatgagagc caagatttaa acgaaatgat cacagattta gagaatcgcc
    18061 ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt tgtttctgag gcacaagaac
    18121 ttcacgatga tttgaaagaa gcttacagct atttctttga agaacgtgaa aaagaaatgg
    18181 aaaaagcaaa gaaaaaagca aacgaagtcg tgtcagaggc ggaagaaaaa gcagaaaaaa
    18241 tcattgctga tattcgtaaa atgcaacagc agattgggca aggaaatgtg aaagagcatc
    18301 agttgatcga tgcaaagacc caattagcaa atctacatca agaagaaact ttgaagaaaa
    18361 ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa accgggagac gaagtgttgg
    18421 ttacgactta cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag tggcaagtag
    18481 aaatcggtat attaaaaatg aatgtgtccg aagacgagct gacgcctgtt gctcctcaaa
    18541 aagagccgac acaacgagtt atccatgctg tacgatctga atctagtagt cacgtgccga
    18601 atcaattgga ccttcgagga aaacgatacg aagaagcgct aagtgaggta gatcaatatt
    18661 tggattctgc cattctagct ggttatcctc aagtgactat tgtccacgga aaaggaacag
    18721 gcgcacttcg caagggaatt actgattatc ttaaaaacca tcggagcgtc aaaagctttg
    18781 agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt aagtaaagct
    18841 tactaaaggt aagcagatgg ttcgctatcc aagtatagaa atgctatact aatcatagat
    18901 tttaaaaatg gaggtctttg atatgtcaca agtaattaca gataagacat ttaatgaaga
    18961 aacagataaa ggacttgtat tgattgattt ttgggcaact tggtgtggtc catgtcgtat
    19021 gcaagcaccg attttggatc agttggaaca agaatatgat gaagaagaat tcagaattgc
    19081 taaaatggat gtggatgaaa atccagaaac tcctcaacaa ttcggtatca tgagcattcc
    19141 aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg ttcattcaaa
    19201 agaacaacta cgtcaaatga tcgatcaata tttataaaaa cttacagcaa aaataagctg
    19261 agggtgggac tgaatccagt tccgtcctcg gtttttttgc ggttgagtta aggttattca
    19321 aattaatatt ttttgagtat aattacgttg tcaggctgga ttgttttttt gacagacatg
    19381 ctataataac tttaattgaa tatgaagggg agtaccagat gaacgaacga atcaaaaaca
    19441 aattagcttt acttcctgat cagcccggct gttacttgat gaaagataaa aatggaacga
    19501 tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat tttactggca
    19561 gccataatac gaaaacggaa cggcttgtta gtgaaatcga agactttgaa tacatcgtta
    19621 cagaatcgaa tatcgaagca ttgctcttag agatcaatct gataaagaaa aatgatccta
    19681 aatacaacat tatgttaaag gatgataaaa cctatccttt ccttaagata acgaatgaaa
    19741 aatatccccg tttggtgatt actcgtaaag tactaaaaga caaagcactg tattttggac
    19801 catatcccga tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga ttatttcctc
    19861 tgagaaaatg caaacctagt cagacgaagg agccttgtct ttactatcat ttgggacaat
    19921 gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta tacgaaaatc gttgatgaag
    19981 tgaaaaagtt tttaaacgga ggacacgaaa aaatcgagtc agagatccat cagaaaatgg
    20041 aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga ataccgagac cagatccgtg
    20101 ccattgagac aatcatgact cgccaaaaaa tgacaaatac tgatttactg gatcgagatg
    20161 tatttggtta tgcgattgac aaaggatgga tgtgtgtcca agtgtttttc gtaagacaag
    20221 gaaagctaat tgaacgagat gtttcaatgt ttcctttcta taacgaagca gaagaagatt
    20281 tcttgactta cctcggtcag ttttatcagg aaaatgagca tttcattcct aaagaagtct
    20341 tgattcctga tacgatcgac aagcccagtg tagaagcttt gttagctacg aaagtgctgc
    20401 aaccaaaacg aggagaaaag aaaaaattag ttgaattggc gaataagaat gcctcagttg
    20461 ctttgaatga acggtttgat ctgattgcca gaaaacagga acggacgatc ggagcagttg
    20521 aacgtttagg aaacgcgatg aatatcccga ccccaatacg tatcgagtct tttgataact
    20581 ctaatatcat ggggacgaat cctgtggctg cgatggtcgt attcattgat ggaaaaccgg
    20641 caagaaaaga atatcggaaa tataaaataa aaacagtcaa cggaccggat gattatgcat
    20701 caatgagaga agtcatctat cgcagatatt cacgagtgat tcgcgaaggg ttgcctttac
    20761 ctgatttgat cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa gatgtcttag
    20821 aaaatcaatt aggaatcgat attcctattg ctggcatggc aaaaaacgat aaacataaaa
    20881 caaatgagtt gctattcggt atagatttgg ctgtagtacc gcttgagcga aattcaccag
    20941 agtttttctt actccagcga atccaagatg aagtccatcg attcgcaatc actttccatc
    21001 ggcagctacg cagtaaaaac agttttgctt cccgattaga tgaaattgaa gggttaggac
    21061 caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc acagcagcca
    21121 cgacagaaga attacaagaa atcggactgc cgaaaaatgt cgcacaaaac gtatttgata
    21181 aattacatca ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc ctatcatttt
    21241 atgtacaaca aaaagagcgt ttacgttttg ttggtaaata ataaatcgct tacgtctggt
    21301 gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa gtcaaggatc
    21361 ttgttaaaac ttttgggaac tacacagctg tcgatcatgt ctctttccaa atccccgatg
    21421 gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa gacaacgact tttcgtttga
    21481 tcttggattt tttgacacaa gaccaaggag aagtgttatg gaatggccat catcttgggg
    21541 agaaagatta tgatgtcatc ggttatcttc cggaggaacg gggactttat ccgaaagtaa
    21601 cgattcaaga ccaactgctt tattttgcag aacttagagg gaaatcaaga aaagagattg
    21661 aaccaaaaat cgatttttgg atggaaaaat ttcaggtaaa agggaaaaag acggataaag
    21721 taaaatcttt gtctaaagga aatcagcaaa aagtccagct gatcgccacg ttaatccatg
    21781 aacctaaatt gatcattttg gatgagccat tcagcgggtt agatccggta aatgctgaat
    21841 tgctaaaaga tggaatattg gaattgaaaa aacatggttc atgtgtcatt ttttctagcc
    21901 acaatatgga taatgtagaa aaaatctgcg atcatttgat catgttgcga aacggaaaaa
    21961 tggttttgaa tggaaaagta catgagattc gtgaatcatt tggccgtacg aagttattct
    22021 tggaatctgg tttaagccaa caggaagtag aagaaatcga aggcgtgaaa aaagtgatcc
    22081 ttcatgaaga caatacgtta gagattacct tagaagatcc tgaagctggg aaagaaatct
    22141 ttactcgtgc gactcagttc ggctacattc ctatgtttaa ccagcaaccg ccaactttgg
    22201 aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta agttttggat tattaccaaa
    22261 gatgtctatt taaaaaatgt taagtcgatt tctttcttga tcatgatatt agtccctttt
    22321 atcatgctgg gtatcattta tgtagcagga aactttgctc aacaaaatag tgatgtcaat
    22381 aaaatcggcg tattggctga taatgagcaa atcagtcagc aaatgagcca agtcaaaaca
    22441 gatgattttg agttcaaagc agttgattct gaagaaaaag cacaaaaaga gctttcggaa
    22501 gaaaaaatcg atgcgtattt agtagtctca actgagaatg ataagataaa tgggatgctt
    22561 tatagcgaaa attcattagg acaaagtaca caactactga tacaacaaca actgactggc
    22621 ttgcaatcga tgcttcgagc aagtcaatta ggcatttcac cagaagaagt agctagcttg
    22681 agccaacaag caagtttgac tcgacaaaaa gtcaattttg attcaaaagg aaaaatgaca
    22741 gttggagaag ataactcgac agtccaatat gtcgtaagtt atgtagcaac gattgtcttg
    22801 ttcattatta ttttgacgta tgcgcagatc attgcacaag agatcgcttc tgaaaaaggc
    22861 acgcggatca tggaagtgat cctgtccagt acgactgcac aaaagcattt ttacggaaaa
    22921 ctaacaggtg ttttacttgt tgcattaacg caaatggcat tatacggtat tatttttggt
    22981 ataggataca gtcaattcaa agatatggaa atggtcaaaa atatattaga cggtatctca
    23041 ttggataact tgattggacc attcttgtgg tattctcttc tattcatgat ctttggcatt
    23101 ttgatttttt ctgttttagc agctttatgt ggctctttgg tcaacaaagc agaagataca
    23161 gcaaaagcaa ttttgccagt gacttatcta tctctaggcg gatatatgct tggattgata
    23221 ctaggagctt cagatccaaa caatatcgtt attcggatca cttcgtatat tccattctta
    23281 tcttcatata ttatgccggt tcgcttagca aatgagacag tcggtatggg tggagcattg
    23341 atttccttgt tggtacttgt agccgtaaca ttcttattga tgatcttgtc tgcaaacatg
    23401 tacaaatcga atgtactcgt gtatagcgaa ggcggattat ggagttcatt gaaacaatcg
    23461 atttcaatta tgagaaatga acgaaaaaaa gcataaaata tagaaaaata tagaaggaag
    23521 ctggacattt attgttccca gcttcttttt tgtatagacc tatttctatt tttttattat
    23581 attggatata tatattcatt gaaaatagag cataacaact ttataatgtt gagtatacca
    23641 atttttggag gcgttttaat ggaccgctat ttagatttac tttcagagaa gttccctaca
    23701 acggaagatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct tccaaaagga
    23761 acagagttat ttttgagcga tatacatggc gaatttcctg cttttgatca tattctgcgt
    23821 atcggttcag gaaatcttaa agaaaagatc aaagaccttt tctctgaaca aatgacggaa
    23881 acggagatca gccaattcac tatttttacg gcttatccag aatatgtgtt gacgactgat
    23941 tggtacaaaa aacaagataa atcccagctg atccatcgtt tgatcgatct tttacggttt
    24001 acgacagtca aatacactcg atcaaaagtt cgaaaaggat tgccgaaaga atatagctat
    24061 atcattgagg aactcttgta tatcgatgat cgtatcaacg ggaaaaaaga ctatgtaaaa
    24121 aaaatcattg aacaacttgt cttgatgaag gaagaagaaa aatttttaac aaaacttgcg
    24181 cagacgatcc aaaaatctgt catcgatcat ttgcatatcg tgggtgatat ttttgatcga
    24241 ggaactcaag cagccaaagt aatggatcga atcatggatt tttcctctgt agatatccag
    24301 tgggggaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc atgtttattg
    24361 acgcttttac ggattgccgc acgctacaac tatttatttg aattagagaa agaatatgga
    24421 ttaaatttac gtccattatt ttcttttgcc catcaaacct atcagaagaa tccagtattt
    24481 acccctaaaa acaggaaaga tctatctgaa agagagcaag aagaattaga aaagatccat
    24541 caagcacttt ctatcctcca attcaaatca gaacatcagt tgttgaatcg acgacctgaa
    24601 tttaaaatgg acgatcgtaa attgttggaa aaaatcaact atgaggaatc aacgatcgtt
    24661 ttagaaggtc agctctatga gttgaaaggg acttgttttc aaacgatcca gccagatgat
    24721 cctaaaaaat tcttggaaga ggaacaaaaa gtgatcgata gtttgatgta ttcattccag
    24781 cactcgttaa gaatgcaaaa acacatgacg tttttgatcg aaaaaggggg aatgtatctc
    24841 acgaacaatc agcatgtact gttccatggc tgtatacctg tagatgaaaa ggggcagctc
    24901 ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggatt ttttgaaaaa
    24961 cagataactg aaagcgcaaa agatttgact gcaaatgaag atttggcgac cgatttgatt
    25021 tggtatgcgt ggagcgggcc attttcgcct ttattcggca agagtaaaat ggcaactttt
    25081 gagcgctatt ttctcacaga gcctcatacg cacgtcgaaa aaagcaatcc ctattatcac
    25141 ttgcgagatg aagagtggtt cagtgaaaaa attttggcag aattcgatgc aaaagaagaa
    25201 ggttcagcaa tcatcaatgg acacacgcca gtaaaagtaa aaaaaggaga gtcaccgatc
    25261 aaagcaaacg gaaaagcatt tgttattgac ggaggtctat ccaaagctta tcaaaaaaca
    25321 accgggattg ctggctactc attactatgc aattcttacg gtttccaaat cgtgacacat
    25381 tatccgttct tgcctattga acaacaattt gcaaaaaaat ctgatcaaac gaatgtaaaa
    25441 aaagtgatcg agcagcagct cccacgcaaa cttaatcgga atacgacaaa aggaatggaa
    25501 ttacagcaac agattactca actccaaagg ctgttggatt atcgaaagga cgattgagaa
    25561 aaatgctagc caacattgct caatagacta aaaagcgact gcaacaaaag gcgtgtcgca
    25621 gtctccttcg gaattagctc aaaaaacggt aaaatattag aagctatttt tctgtttatt
    25681 tctctaatac tcaggtcagg gcgctgattt cacgacctct tccttaaacg gtgttctcct
    25741 gccaactcct cggtcacaag tcacaatatc caacaaacat gagaagctgt tttttgaaat
    25801 tgctccttgt gactcagagt tgaacggcag gctcacgacc taatcagaaa aacgattaaa
    25861 attcattcac aaagaaatcg aataaacgct gttcagatga atctacacga tgagaaagtt
    25921 ccggatgcca ttggacacca aggattttgt gcatattatc tttactttgg actgcttcaa
    25981 tgagtccatc gttggaaaag gcgatggctt ttaacaaagg agaaagatct ttgactgcct
    26041 gatgatgata ggaattcaca aagtatgaat cagggagaag gtcacttaat aaactgtccg
    26101 aaacgatttt gacttcgtga gttgcaaact gcggggcagt gggctgttgt tcgtgtttta
    26161 cggaccattt tggatataat gaaagatctt gataaagtgt tccctccaac acgacattca
    26221 atagttgaag tcctcggcag actgcaaaaa tcggttttct ttgtttcaat gcttccaaga
    26281 tcaatgcctg ttcgaattgg tctcgttgaa tatttgtttc cgtcagtttt ggatgagggt
    26341 cttccatgta aagttgcgga ctaacatcct gaccgcctgc aagaatgagt ttgtcgattt
    26401 tctcaattag ttgaggcgct gaatctggag acatgactgg cagaataaga ggtaatcctt
    26461 gagcatctaa aactgcatcc acaaatccct gcggtgtata gcttacttga tttccttgga
    26521 aagtatctgt cgctcgaatc agttgattcc cggcaatgcc gatgattggt ttcattttta
    26581 ttccccctaa agtagactaa ttgtaacaag gatttaaaaa aatttaaact atgtaagact
    26641 tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaatt ttcgtgtgaa
    26701 attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta agcagaaata
    26761 aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag gacattttat
    26821 tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat gaaaaaagaa
    26881 agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag ctttggaaca
    26941 aatccgaaaa gcagaagaag aaaatgagaa gcgtgctcga accctacggc agcagttgaa
    27001 gacctatgca gaagaaaaag agcaagaatt ggagataatc caaacgaaca gtcggcaaca
    27061 gctgaaacag ttggtagaag aaaaagaaca attcgaaaag caagaattag aaaaagaaaa
    27121 aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagacc aatatcaaaa
    27181 gcatcagagt caagcaatcg gtgcaatcat tgaaagggtg aaagaaactt atggccgtca
    27241 ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg attttacaag
    27301 cagtacaagg aatccaaaat gttgaaatcc gggatctgta tcaaaataga acaaacaacc
    27361 agtgggtaga aaaatatttt ccagatacca cgattattga taaagaagca aaattaagcg
    27421 ctttaggcaa tcggctgaaa gacatccaag aagctatcca attcatacaa caccacggcg
    27481 atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa gacttggaag
    27541 caaattattc cgaagaaaat ttcatgaata aattagaaga agtccttgat ttaaaagaaa
    27601 agtgggaaca gctaactgaa aaacgtgatc gtttagaaga agaggaagaa tggcttcttc
    27661 attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc atttttgaga
    27721 tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactaagt aagcttgatg
    27781 acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg tatgttgctt
    27841 taaaaaaatc agttgctgct atagaagaaa ttgctgggaa atacgggttc aataaagaaa
    27901 cgtatcctta tgaacaaaat cctaaagaaa aattgcaaga aaccaaacag caattatcca
    27961 ttgttcacga gcagcaaaaa aagataacga cagcgttagg tcagtgcagc ggctatatcc
    28021 aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgggaa aaagtaaaag
    28081 aacggtttat ccatgccact tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa
    28141 aacagaattt gatgcatgtg ttaaacgaaa ctgtatcaga agaagatata tacgttagtt
    28201 tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg aaaaaccatc
    28261 cgcttatcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa tatgaagaag
    28321 tagatccaac gccttggatg acaccatttt atcttgtctt cttcgggatg atggtcgcag
    28381 atgttggtta cggattgctg atgctcattg ggacaatact agctcaaaaa ctatttgttt
    28441 taccccgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcggta ccatcgatca
    28501 tctggggatt gatctacagt tcatttttcg gacaggcatt accaaaagaa ctatttggta
    28561 tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt ttgattttat
    28621 ccgtgatttt cggtctgata cagatcttgg ttggattatt cgtggcagca aaggaaaata
    28681 tcaggaggaa agattacttg agtgctgtca gtgatggttt tgcctggcaa gggatcctga
    28741 ttgggatcgt tattgcattg attggtgtca tgttgataaa aaatacagca tttgtttatc
    28801 ttggcgcggg gctatcgatc attgctgctt tatgtatctt aatcgtgccg attatccaat
    28861 cgccgtctaa agtcaagggt gctgccaaag gagcgtataa tctttacgga ttgactggtt
    28921 atatcggtga tttagtcagt tatacacgtt tgatggcact aggtatttca ggcggaagta
    28981 ttggcgcggc attcaatatg ctagttgctt tcatgccgcc agccgctcga ttcagtgtag
    29041 ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg ttaagtgcct
    29101 atgtccatgg tgcacgattg cagtatgtcg aattttttgg aaaattttat acaggaggcg
    29161 gacgtgcctt tgacccgctg aaaacggcgg aaaaatacgt caacatcaat cataagaaaa
    29221 aaaacaataa ataattggag gatttttata caatgatgga ttacttaatt agtcaaaatg
    29281 gtggaatggt atttgcagta ttagcgatgg caacagcgac gattttttca gggattggtt
    29341 cagctaaagg tgttggaatg acaggtgagg cagcagcagc gttaacaaca agtcaacccg
    29401 aaaaattcgg acaggctttg attctgcaat tgcttccagg tacgcaaggg ttgtatggat
    29461 tcgttatcgc ctttttgatt tttatcaact taggaaatga tatgtcagtt gtacaaggat
    29521 tgaactttct aggcgcttct ctgccgattg cttttacagg tttgttttca ggtatcgccc
    29581 aagggaaagt tgcagcagca ggtatccaaa ttttagcgaa aaaacctgaa catgctacaa
    29641 aaggaatcat ttttgctgcg atggttgaaa catatgcgat tttaggtttc gttatttcct
    29701 tcctattagt attgaatgcc taagcataaa ggaggaaaag cagatgaacg ccattgaaaa
    29761 aatcatctct cagatgaatg aagcagcaga gcaagaaaga gcggcgttag agcaagaaga
    29821 gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caaatcgaaa cagaacatca
    29881 aaaacaaaaa gaaaaacaaa tcgaattact tgaaaaaaaa taccgtcagc ttaggaatcg
    29941 ccaacaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt tacgacgatt
    30001 gtttgcagat gcaatagctg aaatggagaa ttgggatgaa tctgaacaaa tccagtttat
    30061 caaaaattca ttgtattcat tacctttgac aggcaaagta acattcattg ctggagaaaa
    30121 atcagcagct tatctttctc agacattgct ggatgaatgg aacaatgaac ttccctttat
    30181 gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa acgatcaagg
    30241 agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa cgatgagttt
    30301 tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata ccacgaacta
    30361 aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt tgaacggatg
    30421 attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat ttacagtcca
    30481 tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga gcagagccgt
    30541 ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat ctatacgatg
    30601 cgctatactt tccataatct gaaggttttg acaaaagccg agatgacaaa tcaaaatctg
    30661 gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga tgccattcat
    30721 acgcaagttt caaatgaact ccctgaaagc attatggaca gtatacgaga agtacgtgat
    30781 tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg acagtttttg
    30841 acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac agaagtcatt
    30901 gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca gaaaagaaca
    30961 tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga agtatttttg
    31021 aactttgtag ataaagatat ggaagattac acgaattttc tattagagac agattatgct
    31081 gattttctgc gccctgtcat ccgtgaggga aatcttgatc tcgtaaagct tgaacagttg
    31141 aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt cggaccactg
    31201 ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg tttactggtt
    31261 atcggcaaaa gaagccattt cgaaccagaa acaatcagaa agcgggtgag acaagtctat
    31321 gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca aattgtttgg
    31381 ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg aagaaatggc
    31441 aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga gcccagaaac
    31501 gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca atcatcaagg
    31561 atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa
    31621 tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca taaaagtctc
    31681 cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca tgtgtctagt
    31741 gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg cgtctattca
    31801 agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc
    31861 tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa tccaacggcc
    31921 attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag ttcaattgcc
    31981 cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg gagaaacggt
    32041 gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac ataaaatcat
    32101 ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat tcactattga
    32161 tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa
    32221 atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg cacccatgat
    32281 cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag cagcagctgt
    32341 acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa aatgggccga
    32401 tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga cggatgtttt
    32461 aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt
    32521 attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta tctatacggg
    32581 aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca tggcagactc
    32641 cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag aaatgcctgg
    32701 tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg aacgatctgg
    32761 aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt
    32821 atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc gggtcgtaaa
    32881 agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt cgatcgattg
    32941 gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc atatccttca
    33001 agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag aacagcagct
    33061 gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc gtctgacatt
    33121 agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg atgatgtcga
    33181 tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga cctttggtga
    33241 agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg acggtaccgt
    33301 atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat tagaaaaact
    33361 agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat
    33421 gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg atggcggttg
    33481 aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga
    33541 ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa atattcgaag
    33601 gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat cctttagaac
    33661 taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga ccgaaagata
    33721 acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta atcaatccag
    33781 tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc gatcatttga
    33841 atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt cctcataaag
    33901 aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat gatttcgccg
    33961 tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg gaagattttc
    34021 gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa
    34081 tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt gcttatgaaa
    34141 agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag gcattgcgag
    34201 agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg tatctttaca
    34261 cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa ggatcggtta
    34321 ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc cctgacttga
    34381 ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag agtgggattc
    34441 agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga actggacccg
    34501 gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc tatgcacaag
    34561 gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct gatgtagaca
    34621 agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa ggattctaca
    34681 cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg atgttgccaa
    34741 gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca gaagggaagt
    34801 gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact cgattgaaaa
    34861 aacagttgac aactgccaca agagggcata aattattaaa agataagcaa gatgaactga
    34921 tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa atggaagagt
    34981 ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg gaacctttca
    35041 tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta gacaaaaata
    35101 tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg acagacacac
    35161 ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc gatcggttta
    35221 ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt caactgatgg
    35281 cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg acgatccctc
    35341 aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag cgcgcagaag
    35401 taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta tcttttttta
    35461 aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc cggctgttca
    35521 gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac ttaccctacc
    35581 attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca ctgcaacttc
    35641 tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga acagtgctgg
    35701 tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga tggttccgat
    35761 tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta tattaaaaga
    35821 agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca tcctaaaatt
    35881 ggcctttgtt atccaattga ttggtacggt tgctttatct tttgtatttg tgcctgaatt
    35941 tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt tttgtaatgc
    36001 agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt atttgatcat
    36061 ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc gtgatttact
    36121 ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa ccgttacagg
    36181 taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct
    36241 agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg tgacacctag
    36301 aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc tgattttaac
    36361 gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat tgaaaacaac
    36421 tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta cgagagcgga
    36481 agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt tattttttgt
    36541 tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa cgatacctaa
    36601 aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta cagtaggtct
    36661 tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta tagcgcttat
    36721 gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa aagcacaaag
    36781 aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat ttcatcttct
    36841 gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt gcttcttgga
    36901 tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt gaacctcggg
    36961 tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt tatcaatagg
    37021 ttataattca tattttttta ttttttcttt tacgatactt agtccagaag ctaaaatttc
    37081 aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac gtgtagtaaa
    37141 gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa ttatcttagg
    37201 gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg ttggtttttc
    37261 tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta atttagtttc
    37321 taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca catctaatcc
    37381 agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga catgtactgg
    37441 aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc cagctaagta
    37501 gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt gatagtaagt
    37561 tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt caatcacttc
    37621 ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat tacaagaagg
    37681 cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata tatcaggtcg
    37741 cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact gagccaattc
    37801 aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag agaaaagtgc
    37861 agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag attctaagga
    37921 cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc
    37981 tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag ttgattgaat
    38041 aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa aatcaatgat
    38101 atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg gagaaataaa
    38161 tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt tataagcctt
    38221 gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg agggtaactg
    38281 agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa tcaacgatcg
    38341 ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt aattgtcata
    38401 agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt ttaccattga
    38461 ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga aaattattaa
    38521 ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg
    38581 tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg aaattttacc
    38641 tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg agaacagata
    38701 ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt taccttctca
    38761 tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat tgataaacgc
    38821 aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag attacttagc
    38881 aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat tgtcgcattt
    38941 gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata ttttagaaga
    39001 aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa cgtttataaa
    39061 tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt tgacctctag
    39121 taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca atgctgtggg
    39181 cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt ctcaaatcta
    39241 tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag ccatggcaga
    39301 aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt ctgagaaaaa
    39361 aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc tagctattga
    39421 agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt taaaatgaac
    39481 ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg
    39541 gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac
    39601 aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct tagatggttt
    39661 acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc atttgtttta
    39721 tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag cggtaatcta
    39781 tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat gccgaaaaaa
    39841 gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga agcctactta
    39901 gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt gcagccagat
    39961 tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc agaaagtgtg
    40021 caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga agatccagta
    40081 tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa taaaacaaaa
    40141 ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt aaaagaagat
    40201 cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt tatttgattt
    40261 ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct acaatggttg
    40321 ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat tttaattatt
    40381 ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc ctcggatgac
    40441 ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa
    40501 tccttttaag tcagtaatct caagaccggc agctttatgt tgctcgtgta atttttctag
    40561 atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg caccatgatc
    40621 atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat aaacaagtgt
    40681 aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg ccttttgata
    40741 aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca ttttcataga
    40801 ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa atagttgatt
    40861 tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac tgttaaggtg
    40921 gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg attatcaaac
    40981 ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa tcgaggctcc
    41041 aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga aagaagaagc
    41101 gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt cttatctggg
    41161 agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata atccaggata
    41221 tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga ccaataaaat
    41281 ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc
    41341 agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc
    41401 tcagtttttt tatttattac agtacatatt tt
//
LOCUS       DAYYGS010000016        37817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000016 DAYYGS010000000
VERSION     DAYYGS010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.016"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            476..1339
                     /locus_tag="ACHA44_000789"
     CDS             476..1339
                     /locus_tag="ACHA44_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8904102.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="ACHA44_000790"
     CDS             complement(1468..3090)
                     /locus_tag="ACHA44_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8904103.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="ACHA44_000791"
     CDS             3339..3860
                     /locus_tag="ACHA44_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8904104.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA44_000792"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="ACHA44_000792"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HFM8904105.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="ACHA44_000793"
     CDS             5941..6759
                     /locus_tag="ACHA44_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HFM8904106.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="ACHA44_000794"
     CDS             complement(6862..7059)
                     /locus_tag="ACHA44_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904107.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="ACHA44_000795"
     CDS             7228..7806
                     /locus_tag="ACHA44_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8904108.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="ACHA44_000796"
     CDS             7901..8038
                     /locus_tag="ACHA44_000796"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904109.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8851..9354)
                     /locus_tag="ACHA44_000797"
     CDS             complement(8851..9354)
                     /locus_tag="ACHA44_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8904110.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9713..10738
                     /locus_tag="ACHA44_000798"
     CDS             9713..10738
                     /locus_tag="ACHA44_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8904111.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11061..11939
                     /locus_tag="ACHA44_000799"
                     /pseudo
     CDS             11061..11939
                     /locus_tag="ACHA44_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
     gene            12415..12531
                     /locus_tag="ACHA44_000800"
     CDS             12415..12531
                     /locus_tag="ACHA44_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8904112.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13459..13797
                     /locus_tag="ACHA44_000801"
     CDS             13459..13797
                     /locus_tag="ACHA44_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904113.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13891..14121
                     /locus_tag="ACHA44_000802"
     CDS             13891..14121
                     /locus_tag="ACHA44_000802"
                     /inference="COORDINATES: protein motif:HMM:NF042636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6568 family protein"
                     /protein_id="HFM8904114.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14521..14892
                     /locus_tag="ACHA44_000803"
     CDS             14521..14892
                     /locus_tag="ACHA44_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HFM8904115.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15166..15858)
                     /locus_tag="ACHA44_000804"
     CDS             complement(15166..15858)
                     /locus_tag="ACHA44_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8904116.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15861..16646)
                     /locus_tag="ACHA44_000805"
     CDS             complement(15861..16646)
                     /locus_tag="ACHA44_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8904117.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA44_000806"
     CDS             16883..18283
                     /gene="lacG"
                     /locus_tag="ACHA44_000806"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HFM8904118.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18308..18625
                     /locus_tag="ACHA44_000807"
     CDS             18308..18625
                     /locus_tag="ACHA44_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8904119.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18638..20311
                     /locus_tag="ACHA44_000808"
     CDS             18638..20311
                     /locus_tag="ACHA44_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HFM8904120.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSGMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20325..21956
                     /locus_tag="ACHA44_000809"
     CDS             20325..21956
                     /locus_tag="ACHA44_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-beta-1,4-galactanase"
                     /protein_id="HFM8904121.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHA44_000810"
     CDS             complement(22447..24012)
                     /gene="guaA"
                     /locus_tag="ACHA44_000810"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8904122.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHA44_000811"
     CDS             complement(24071..24994)
                     /gene="coaA"
                     /locus_tag="ACHA44_000811"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFM8904123.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            25190..25795
                     /locus_tag="ACHA44_000812"
     CDS             25190..25795
                     /locus_tag="ACHA44_000812"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8904124.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            26103..27119
                     /locus_tag="ACHA44_000813"
     CDS             26103..27119
                     /locus_tag="ACHA44_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8904125.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            27260..28561
                     /locus_tag="ACHA44_000814"
     CDS             27260..28561
                     /locus_tag="ACHA44_000814"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HFM8904126.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28607..29269
                     /gene="deoC"
                     /locus_tag="ACHA44_000815"
     CDS             28607..29269
                     /gene="deoC"
                     /locus_tag="ACHA44_000815"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFM8904127.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29290..29688
                     /locus_tag="ACHA44_000816"
     CDS             29290..29688
                     /locus_tag="ACHA44_000816"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFM8904128.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29754..30836
                     /locus_tag="ACHA44_000817"
     CDS             29754..30836
                     /locus_tag="ACHA44_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8904129.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            31048..32610
                     /locus_tag="ACHA44_000818"
     CDS             31048..32610
                     /locus_tag="ACHA44_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904130.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32603..33736
                     /locus_tag="ACHA44_000819"
     CDS             32603..33736
                     /locus_tag="ACHA44_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904131.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33736..34692
                     /locus_tag="ACHA44_000820"
     CDS             33736..34692
                     /locus_tag="ACHA44_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904132.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34791..35957
                     /gene="deoB"
                     /locus_tag="ACHA44_000821"
     CDS             34791..35957
                     /gene="deoB"
                     /locus_tag="ACHA44_000821"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFM8904133.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35981..36799
                     /locus_tag="ACHA44_000822"
     CDS             35981..36799
                     /locus_tag="ACHA44_000822"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8904134.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36816..37523
                     /gene="deoD"
                     /locus_tag="ACHA44_000823"
     CDS             36816..37523
                     /gene="deoD"
                     /locus_tag="ACHA44_000823"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFM8904135.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT        11788 a         6622 c         7986 g        11421 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgatag gagatataca acagaaatga ataaatatta
     8281 acgtgttttc aaacagtata aaacggtgtg gtttttggaa taaaaaaata acatatctca
     8341 ttttacactt ttgttatttt atacttaaaa aataacgaca cagaaaatac cgtgtctcag
     8401 atttctctac tacttctaaa aactactgtt tttttccttt gaaatgcctt caaaaagtag
     8461 gatcaggcag aatatactgg cagaatcaag gagaagggag cctaaataag gagaaaaacc
     8521 atagaaaata taaagtaatt acttataaaa caagcttttt atagaaaaat aatatacctg
     8581 agtagggtat aaattaagac ggaattcctt tctagattga gatattgact aggaagaatg
     8641 ttaagatttt aagcctttaa gctaaaaaaa aacccttttt gttaaataag gggaatatag
     8701 cctgtcagtt tcaaactgca agtcgtcctg ctgcaataat atagtagatt agagatgaaa
     8761 atagattttt ttaaaccaat gactgaataa aaagaccgtc atagatacac attggaatgt
     8821 gtctattacg gtcttttttt acgaacgatt ttattgaaga cggacagttc gggtcaaaaa
     8881 taaaaaagct acgatcagaa ataagaataa aatgtatccc aaaataatgg ctagacggtc
     8941 taacttattt gtcgatagat gtttgtttag aagttgatag gcccataaac ctatcagtcc
     9001 tcctgttgta ttagttatca aatcagtgat gtccgctacg ccaataccga ataggaattg
     9061 aatcaattct gccaagaagc ttacaaggaa aataatccct aattttttag aaaaagaaag
     9121 tctcttgaaa ttgacattca aaagcacccc aaaaggaaaa aagacaatca agttataagt
     9181 ggtctcacgt aataccgtcg tattttcaaa agaaaaagta gaaaaaggga cgagattgac
     9241 attgctgtaa gaataagtga gaaattgtgg aagattaaat gatagcttga ataaaatcaa
     9301 ccataataga aagaataaat aaatccctaa taaaaatttt gatacatacc gcatagacat
     9361 ctcctccatt taaaaagtat accatcgaaa accgctgaaa aatcataaaa aagtatgaag
     9421 tttaaggacc aacgtagtta tcgtacgaaa attagaagct tttttttgtt gggaatccgg
     9481 tttctttaac aaagctaaga tagcgagttg cttggaaaag ttgctatctt ttttctgtca
     9541 aaacgactcg gaatgttcgt tttttgttct aatttctcca agttgtttac tttttgatac
     9601 taagtttatg aaaaatcaag aaatagttgt tatactaggt aatgctgaca aacgggattg
     9661 gcatttcatc ttggttcagc tttttttcca attactagga aggagggata gattgttcaa
     9721 gacttatttt cgtttcaata aaatacaaga taatcctttt cgtatcatga atctgatgct
     9781 acttatcttt attgtattat ggttaggtta cataaatcat gattttttga tttcaagttt
     9841 ttcatcatta gggatcttta cttttttcta ttatcaaaac ataccgatga aatatttgct
     9901 gaaacgtatg tttctgatcg gttgcggact gttggttgct ttcgttttgg ggatcttatc
     9961 gacttatgtt ttatggcttg aaccatttgc cgtagctttg gttgcttttt ccagcagatt
    10021 cgtgttgcga ttattccata tttctaaacc aggtggcttg ttttttgcga tgctttctgc
    10081 catgggaacg agcatgcagc tgccaatcgc tcaattaccg atcgtctcac tttatttttt
    10141 tatgggtgtc gtttttgccc tgattgcggc agtgattaca aaattactgg attcacggcc
    10201 tgagcagaca attgaaaaag caactttaaa agaaagattt cacgaagagc ctttagtgat
    10261 cattgattct gttttctaca gcgctgcctt atttctttcc gtttatgtca gtcacggctt
    10321 gaatctgcat aatccttatt ggttgacctt gtcatgtgca tcgattttgc tagcagaaaa
    10381 cttggatgcg atgaagcatc gacaagtcca atatctgatt ggatcgatgg gtgggttatg
    10441 tgtgtcggca ttcttgtcat ttgtgccgtt tacgcagttg cagaccattt ttctgatcac
    10501 ttttctatat ggaatcgcac aatttctagt tgctagaaat tatgcagttg ctaatatttt
    10561 tcttaatccg atggcgttga tgctatctac acttattcgt ggagcatact tgatttcttt
    10621 gatcgaatat cgttttttag ggatcgtcat aggaagtttc atcggtttag gtgttgcgtg
    10681 ggtaatgacg gttggtttgc aacattattt aacggttgtt cgtcaaaaat tagaatagaa
    10741 ggacaaatat atgtgccaaa agaggttatg acaagatttt tgtcacaacc tctaattcaa
    10801 ggtgttcaaa agccagattc tcagcattaa gtcaaattca cacaaaaatg tggaaaggaa
    10861 ctttcaaaaa aattttatat ttattctatc aaaaaatttt gtttatgatg gtaactgaat
    10921 aaactttcct gagatggtca atgctttcct tgaaagtctt acagatattc tttcttcata
    10981 gaaagtttaa gttttatagt tagtgtacaa taaaagtatg tttataccat atggtgtccg
    11041 gataatcagt catttaaaca atgaggttca aagatatgaa aaggggaaat ttgatgaaga
    11101 aaataagcaa tgaaatccaa attctggcag aaactttaac tcttcccaaa gtcgttatag
    11161 agcaaatgat tgtacaagca aaaaacatcc ttgatgaggt gtttgagcaa tatagtcatg
    11221 aactaatgga tcctaataaa gcaaaggcga ctgcaaagaa aatagacaaa gtctatgaag
    11281 aggaaccttt tgcagcatta gctatttatc tatatgcagc ttctcaaagt tggaaaaaaa
    11341 tttatcaacc attgaagatt ccaagaacta tctatttggc aacaatgaat gcttttacta
    11401 gatttattca tgaacatttc caagtaacag gaagctatcg ctttgaccgt ggtttttgga
    11461 tttggcatta cactagtggc ttgatctttc ggattggtga attggagtac gaaatgacat
    11521 attttccgaa agggtacacg gatctaagat tagaaggaaa gcagtgcctt tctcttcata
    11581 ttccttccga tgcagattta tctcagaata agataagaga tagttacgaa agtgccattc
    11641 gtttttttga actttatttt cctgattacc attaccaagt gatgtacaca gatacttggt
    11701 tactatcacc caatcttact aaatagttga aaaaagagtc aaaaatctgt ctttttgcag
    11761 ccgattatcg attattatca gttgatgaac aggacgatag tggagtccct tggatctttg
    11821 gacgagtaga tgcccaaata catgattatc cagaaagtac gagtttacag cgacaagcaa
    11881 aagagcaatt gcttgcaggc gagcacattg gctctggctt aggtattctt acttcataag
    11941 tttagtactt cgaaatagtt taaaagagcg tgagacaaaa gtaaagaaca cttttgtctc
    12001 acgctctaaa cacgtataaa cggcggaagc agaagcaact ccttcggaaa taagctgaaa
    12061 ttcacaaaaa tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc
    12121 ttctgtccca gcctcttttt tagtgttcta tcgaacacac agtacgttca ataggtaaac
    12181 tagctataac aatagatatt aaagttaacg gacaagaggg ccttttttag gtagctaaaa
    12241 cgtaataatg aaaatttatt tccagagata ggagtcaatc agctagtctg atcgactgtt
    12301 ttaacaagtt gactttcatt ttaacttttg ataccattta aaacacaaat cgtaattgtt
    12361 tcgatttgtg ttttttagca tgactacaat caaagaagaa agtgggtcgt ccatatggaa
    12421 aaaacagata tctcaagtgc ataccgccgc ttgaaaagcc caaatatcaa aacacgaaaa
    12481 cgtgcgctga agatcatcaa agatgtgaaa cggaatagtg ggaaaaggta agaagaagga
    12541 aacttaatta aaaaaatagc cgaaaaacgt ttttcggcta ttttcaataa tcggtttttg
    12601 tgtaaggttt ccgaaagaac tgctgttgat tttccatttt taacatatga taaattggta
    12661 gaatctttct tttcaaattg ataccattcc ctaattccag ttatatagtt aactacagaa
    12721 ccacaaagca cgtacgttta taaaagtatt atctgtagtt atttttcaat tccctttcaa
    12781 cctcttattt tgaccagtag tattgatcac tttacctttt aacacctttg ttcgtttttg
    12841 aaacattttt agtcaagaat ggaaacgtaa ctacataatc aaaggttttg cgaaatattt
    12901 catgaaatat ttcatgtaga agcgagcaat acggttgcaa ataaccatta tcgtttaata
    12961 agcagttacg aaagctccca atatctctaa cgttcagctc actccatttc ttttcttcaa
    13021 aaaaatcagt gattcgtatc tctttctatt tgtcgaaaaa gttaacttta aaaagtaacg
    13081 ccgaaaaaat atgcaaaaaa ataaaaaagc agttactatt gctagaataa catcaaggaa
    13141 taataagttt tggttttcag ataatagtcg ataagttgtg tattggaatt ttagatacag
    13201 taataaagta tttttcatga aattttgtga gaatcactat tacaagtatc tttaataaaa
    13261 ttaatggtta aggaggaact taaagtagaa tgtaaatgaa tagttgtagt cagtcgattg
    13321 tgatactaga ctcaaagttg caggaataag tttttcaatt tgtgattgga agataaaaaa
    13381 tgaaaaagaa gggctttttt ttatactttg ttgattgtag tgtttatttt ccctctgttt
    13441 agccaagtaa cattttctat gactcaacca gtttcaagtg aggaacatga taaccttttt
    13501 cgtgctacag aagagtttaa gaaatatcag gaaaatattg ttccggagac tccattagct
    13561 agtgagccaa ttactgatat tgatggaaac caaataggat ggtatataca gtacgaagtt
    13621 atggtggtaa ataatgactc aatagtaaaa aatacagtag gcttagagct aacaccgatt
    13681 gaaaaggtga gttcattgtt aacgtatatt tatactgatg aaagtgacga gactgagatc
    13741 attttaacgg attactcaaa aattgctact gataaaaagt atatattgtg gatgtagaca
    13801 ctagtgaaat actttatgat atagaaattg atgaaaattt actagatgta gcacaagaaa
    13861 tcgatcatca aaagcaggaa gctattcgat atgctggtag aaaagggaaa agaaaatatt
    13921 tattacgtga atgtagcaaa agttagagaa gatgaaaatg agtggaaaga attcaagagt
    13981 cgctactcaa ttaactcgac tccgacattt acagtttaca gagaaggatc gatagaaaaa
    14041 actgtttttt ggactaaaga aagtggaatg tctttagctg aagtagaaga gtttttggat
    14101 tatgttagta tgcaacagta agggagttct tattatgaaa aattttgaca catattatgt
    14161 tattgttttt tcgatatctt tgctgttaat attactaact ttaatgcagt ataaagagta
    14221 tccagagtat tttattttaa cttttgtata tttagtaagt agctacacac ttctatataa
    14281 attcagaagt tcttctagag tttctaaagt ttgtttctta attagcttat tgattctata
    14341 atatttttga tttatatagg aaattaaaaa tattatagaa tcaacactca gagaacgggt
    14401 tggtttttaa tagatttaat aagtactgat aatgaaattt ctcctggttg ggaaaacgaa
    14461 gttggttacc ctgtagaaga attttttcaa gattactttg cggagtatgc cgataggata
    14521 atgagagatt acgtaacaga taaaactcaa gaagtcatca atcaatccca gtcattaaat
    14581 gaattatctg ataaggcgaa agaagaagca gttgccatta tttcttcagg gattagtgac
    14641 tatattgata tgagtggtga aatcaattat ggcgataaag acaaaattgt acgcatgata
    14701 aaagctttta tcaaaacttt tgatgtgaag gcagatctta agtttaatga tacttaccta
    14761 gtaggaaaat taattaatat ccttgggaca gagaaagata cggaaacgtt tatttcctct
    14821 ttgtctatgt tgtttacaga taagcatttt tcaactcagc ttggtggaga ggaactagtg
    14881 gaaatctttt aaccattacg cccagcatta ataaaagtat atcaaaaaag tactcctgat
    14941 gatttttaag attggtttga gaaataatgt gaaaacacta tttttaatgt taattataga
    15001 tagagatgaa cgcgtcatat ggttaaaaga atgaaaaagt caaagataca ctgctatatt
    15061 tcactaagga tatagcagtg tatctttcat gtcgtttcac tggcagtttt ttataaaagg
    15121 aggtgtgcat ttcagttgct tttctttggt agttagttga atcgtttaag aattaacgga
    15181 tgtggcagac aggtgctctg tccaaaaacc agtaaaatct ttgatggttc catctttatt
    15241 tagctgtgcc tgttcaaata ctgttttgtt tttaccgaat ggatcaattg caataattgt
    15301 tccgatcccc gcacgtttgg cagcaagtaa tcctgagtaa gcatcttcga taacaagaca
    15361 atcagccgga gctaagctca gtttgtctgc cgctttaaga aaaatatccg gttgcggttt
    15421 cccagggaaa gagccgtcgt catagacaat cttgtcccag tcaaaccagc gggctaaatc
    15481 gaaaatatca aaataaaaag caacattctc tttgacagta gccgtggcaa tcgttaatgg
    15541 aatctgttgg tcagtcagct ggtcaagagt cgcttcaagc ccttttgtca actgaagctc
    15601 gttttcattt tgcaagcaaa gcatacgata atagctttct ttttcttctg acaatacggc
    15661 gatctcagag gcggtcaaat gctctgaaat gaaatgtttc aaaatctccg tattcgtccg
    15721 accatgaata ttttttaaaa tatcctcgtc tgataaacca tcttttgtat gatcatgtat
    15781 catatgcagc caagcagctt catgcagatg agagtccaaa aacattgttc cgttgaaatc
    15841 aaatataatc cctttcatag ttacgctcct atagtggtat tttcggttga atcagcaatt
    15901 tgcagatcga catattgtgc atagtgctct tgaacctccc gcgagatctg gcgatccgta
    15961 atgagcagat cgaaatcatg caaattataa aacacataaa aatcatctcg attgaatttt
    16021 ttattatcaa cgagtagata tttcttacgg gaattattta gagcgatctg ctgtgcttct
    16081 ccttcttcgg tactgtaagt agagatcgct tcgttgtgaa cgccatttga actgataaat
    16141 gcttttgtaa actttagtct tcttaaattg tcgttggtga tcggtccgac aaatgcgccg
    16201 gtatttttcc ggaaatcacc gccaatcagt agaatttctg gtccatctgc ttgagcaaga
    16261 atatcaaaaa ctggataatt atttgtaatg atccgaattt tcttgttcag taaatgcgtc
    16321 gccaatagtt cgattgttgt acctggtcct aagaaaatcg tttctccctc gtgaatcaag
    16381 gaggctgctg tggcagcaat ttcttttttt tcttgaatct gcagagtggc tttttcagtg
    16441 tgagaaagtt cttgatctaa agtgtaggaa agactttgag cccctccatg gacacgcagc
    16501 agcttgcccg atttttctaa ttcatctaaa tctctgcgaa cggtcatatc agaaacatct
    16561 aattgattga tgatttcatt aacggtaagg attccttttt tattgaccat ttctacgata
    16621 gtaagcaatc tttctttttt caacattgtc ttcacctcta atttaaggat aatctatcat
    16681 ataaacggac aacagtcaat ctaattcgaa caaaagtaaa catttagggt ggtttttatg
    16741 tttgttacaa tagattttat tgctgttttc tggtttttat gattatttgt gtttgttttt
    16801 gttgacaaat gttttggatg cgtttacaat gcaatcagtt agaaagagcg aaaactgatt
    16861 atagatttga ggagagatgt acatgcttaa attaccagaa gattttattt ttggcggagc
    16921 cacagcagcc tatcaggttg aaggagccac taaggaaggt ggcaaaggcg ctgttgcttg
    16981 ggatgacttt ttagaggaac aagggcgatt cagtcccgat ccggcaagtg atttttatca
    17041 tcaatatgca aaggacattg aattgtgtga acgttttggt gtaaatggtc taagattatc
    17101 cattgcttgg agccgcattt ttcctgatgg agctgggaaa ccaaatccag aaggaatcgc
    17161 tttttatcat cgtgtgttcg aagaatgtaa gaaaagaaac gtaacgccgt ttgtgacgtt
    17221 gcatcatttt gataccccta aacgtttgtt tgaccatgga gattttttga atcgagaaac
    17281 aatagaagca tttgtttcct atgccatttt ttgttttcat gaattcaaag aagtcaaggt
    17341 gtggagtacc tttaatgaaa tctatccagt tgcaaccaat caatatttgt taggtgtttt
    17401 cccaccagga atcaaatatg attttacaaa gatcgtcgcc tgtcttcata atatgatggt
    17461 tgctcatgcc cgtgtagtga actatttcaa agaaaacgaa cttccgggtg aaattggtgt
    17521 agttcactca ttagagacga aatatgcggc aacagacgcg ccggaagata agcatgctgc
    17581 ctttttagac gatgcattgt cgatccgttt cttattagat gctacctatt taggctatta
    17641 ttcaactgaa accttgactg ctcttgatga gatatgtgaa gcaaatcagg ccagctatca
    17701 ttttcctgaa gaagatttcg ttgaattgaa aaaagcatcc acgcgtaatg actacttagg
    17761 gatcaatcac taccaatgcc attttgtcaa agcttacgat ggagaaaatg caatccatca
    17821 taacggtacg ggtgaaaaag ggacctctgt ttataaagtg aagggaatcg gagaacgtat
    17881 ttataaagaa ggaattcctc gaactgattg ggactggctg atttatccag aaggtctata
    17941 tgacttgttg ctgcgaatta aaagcgatta cccacattac aacaaaatct atatcactga
    18001 gaacgggatg gggtataaag atcagtttga agatggcatt attatggatc agccgagaat
    18061 cgactatttg agagtctatt tggaatcctt aagcaaggcg ataacagccg gggtaaatgt
    18121 gaaggggtat tttttgtggt cgcttatgga tcttttctca tggacaaatg ggtataacaa
    18181 aaggtatggc ttgttttatg ttgattttga aacccagaag cgttacccaa aagaatcagc
    18241 ttactggtac aagttggtca gtgaaaccaa aacgattatt taataagtga taatggagga
    18301 agaaaagatg aataaagagg aattacaaat gcttggtttt gagatcgttg catattcagg
    18361 cgacgctcgc agtacattat tgaaattatt gaaagaagta agacaaggaa actttgaaca
    18421 tgttgattcg gcgttgaaag aagccgacga aaatttaacc cttgcacata attcgcaaac
    18481 aaaaatttta gcggaagaag catctgggaa agagatggag atgggattca tttttatcca
    18541 tgggcaggat catttaatga ccactttatt gctgagagat ctgattcaag actttattgt
    18601 actgtacaga cagaatcaag gataggagaa gaacgctatg gatggaatca ttaaacaaat
    18661 cgaaaaaggg aaaccatttt ttgaaaaaat ctcacgtaat agttatttag gagccgttcg
    18721 tgacggattt ttaacagcga tgccagctat tttattttca agcattttca ttttgattgc
    18781 tgcggttcct gagatttttg gttaccaatg gcctgaatct gtctccacat ggctatggaa
    18841 agtctataat tattcgatgg gtgttgtagg gttactcgtt tcagcaacca ctgctcgctg
    18901 cttggctgat tcgatgaatc gtcgcttacc gaaaaacaaa gtaatcaaca ccacctctgt
    18961 gatgctggcc tctgtggtaa gtttcctgtt gttaagcgtc tctccgattg aaggtggatt
    19021 ttcaacggaa tatatgggga caaagggact actggcttcc tttgtcgctg catttttaac
    19081 agttaattta tataagtttt gtgtgctgaa agatattacc attaaaatgc ctaaagaagt
    19141 tcctggaacg atttcgcaaa ccttccgaga tatttttcct ttctcttttt cagtatttgc
    19201 ggccgtttta attgatttag gcattcgaca aatcttccaa atctcatttg cagaagcaat
    19261 cattacggtg ctgcagccgc tatttacagc agctgacggg tacttaggaa tcgctattat
    19321 ctggggagcg atggcgctgt tttggtttgt cggtgttcat ggaccgtcga tcgtggagcc
    19381 ggcaattgcg gcaattatct atgccaatgt ggaaaccaat ctgcaattgt tcaattccgg
    19441 tgaacaagct tctaaagtac tgacggtggg tttagggaat tttgtaggaa ctatgggagg
    19501 aactggggca acattggttg ttccttttat cttcctactc tttgctaagt ctaagcagtt
    19561 gaaggcagtt gggaaagctt cctttattcc agtaagtttc gctgtcaacg aaccattgct
    19621 atttgcggca cccatgattt tgaaccctta tttttttatt ccatttttgt taacacctgt
    19681 tgtaaacgtt tgcctgttca agttttttgt cgatgtattg ggcatgaaca gctttatgta
    19741 cgttttgcct tgggcaactc cagcaccgat cggtttggtt ctaggaacgg gcatgggtat
    19801 cttagctttt gttctagcag cggttctaat cgtcgttgac ttcctgatct atctaccatt
    19861 ttgcaaagca tacgatgctg tcttagtaga gaaagaagca caaaaagcag aggtgatcgc
    19921 aaccgaggaa ggtgcttctg ccgatgtacg tgaggaagta cctgtggcag cgagtcaagt
    19981 tatcagcgat gcaaataaag aaatcaatgt gttggtactt tgtgctggcg cagggacaag
    20041 tgggatgtta gctaatgctt taaaaaaagg tgcatctgaa tcgaaagcac cgatcagtgc
    20101 aatggcgggt gcttatggtt ctcattacga aatgctgcag gattttgata tggtggtctt
    20161 agcaccgcaa gtcaattctt actatgatga tatcaaagaa gacaccgatc gcttaggaat
    20221 caagctggtt gcgacaaaag gagcagaata tatcggatta acgagagatc ctgaaaaagc
    20281 tgttcagttt attctgaacc aatttcaata aaggaggagc cataatgaaa atgggaattt
    20341 ggttgcgtaa agctgtttta tttgcagctg tgttaggggc tttgggggct gtttcaaaaa
    20401 ctgttgaagc atctgccagc gaatttattc gcggagcaga tatttcgatc ctgtcagata
    20461 tggaaaaatc tggtgcaaaa tattatgaag acgatatttc aaaagatgcc ttagagattt
    20521 taaaaaataa tggtgtcaac tatgttcgac tgcgtttgtg gcaggatcct tataatagta
    20581 atggagaaag ttatggagca gggacaaatg atctggaaac gacgattgac cttgcaaaac
    20641 gagcaaaaaa tctgggactt aaggtgctgc tggattttca ctacagtgat ttttgggttg
    20701 atcctgggaa acagaatctt cctaaagcat ggcaaggtct gacctttgaa gaaatgaata
    20761 cggcattgta cgactataca aagaacgttc tgtcggaaat gaagcagctg gacgtttatc
    20821 ctgatatggt tcagatcggc aatgaactga atagcggaat gctctggcct tatggcaagt
    20881 cttggggaga aggcggggga gaatttgatc gattggctgc ttttttgaag tctggtatcc
    20941 aagctgttcg agacactcaa cctaaaacaa ctccagtaat gctccactta gctgatggtg
    21001 gcgatacagg agcatttact tggtggtttg atgaaatcac gagtcgaggt gtgtcatttg
    21061 atttaattgg agtgtcgtat tatccgtatt ggcacggttc aatgaatgaa cttcaaacca
    21121 atatgaatgc gatcagccaa cggtatggta aagaagtgat cgtagttgaa acagcttatg
    21181 ggcatacaac agcgaatgcc gatactatgc caaatgcttt tggtgaagcc gaagcagctg
    21241 cggggggata tgaaccaaca ccgacaggac aagcagagta tttgctggat cttgccgatc
    21301 ggattcaggc agtaccgaat aatcgcgggg cgggcttctt ctattgggaa cctctttggt
    21361 acaacggaaa tgtgagttgg gcaacacaag caggtatgaa ttatctcagt gttcaatcca
    21421 ctatgggaaa cgaatgggac aaccaagcga tgtttgattt tgctggaaac gccttgccaa
    21481 gcttgcgtgc gtttaaacaa gcaggggcgc aaataaatct agttaaaaat gcaagtttcg
    21541 aaagcgatgg accaacgacc agtccttcca attggcaaac ctggtttcaa caaggaaccg
    21601 ctaagggtac gatcaaaacg gaagcgggca ctttttttgg ggactacaaa ctaacttttt
    21661 ggcaggatca agcatacgag gcatcagctt atcaaaatgt tagtggcata agcaaaggaa
    21721 cttacacatt gagtgcctat gtaatgtctg ggggagagtt tgatactaat cagttatata
    21781 tcaaaaatta tggtggagcg gaactaaata ctgcggttgt taaaacaagt gaatggacaa
    21841 aaattacaat caaagacatt ccaattacaa caggtgtttg tgaaattggg gtttatagca
    21901 aggcttcagg aaatgcttgg acgaacgttg attttgtttc attggtgaaa caatagactg
    21961 cggatcgttt cgattcaaga tcggaggttc ccttcagcca catcgttagc gtagaaggca
    22021 ggaatcgaac ctgcacctcg cctttgccaa aaataaccag aaacgatttc cagtcatttt
    22081 taccaaaacg agtaaagcac cagacttcat ctacactttg tattatgcta aagagagttc
    22141 aacgatttcg taagaagcaa tgaaaaaatt gcaaaaagtt gtgcagataa cataaagttt
    22201 gtttaagaaa gggtaacctt agaattccgt cgtgaatatg atttgagcac ttagatggat
    22261 agaaaaaagt tctcggaaaa cagttcgttt ttcgagaact tctttttatg ataagaatga
    22321 aacaaattaa cttatattat agtctctttt ggtagggaac catcttattt ttaatgaaaa
    22381 tcaagcattt tagctattga agaatgttga tttataggga ttccccctcc atattatgtt
    22441 ggctctttat tcccactcca ctgttgctgg tggtttagaa gtgatgtcat acacgatacg
    22501 gttgacgtgg gctacttcgt ttacgatacg tacagagatt ttttgcaaga cttcccatgg
    22561 gatacgggca aagtcagcgg tcattccgtc gattgaagtt acagcacgga taccgactgt
    22621 gtagtcgtaa gtacgtccgt cacccataac accgacagag cggatccctg gaagaacagt
    22681 gaaatattgc cagatatcac ggtcaagtcc agcagctgcg atttcttcac gcaagatagc
    22741 atcagattcg cgtacgattt ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc
    22801 aagaccagga ccagggaatg gctgacgcca aacgattgaa tcaggcatgc ctaactgagt
    22861 ccctaattca cggacttcgt ctttgaataa tgtgtttaat ggttcgatca attcaaattg
    22921 catatcttct ggcagtccac ctacattatg gtgagatttg atcgtctgtg ctgtttctgt
    22981 accagattcg ataacgtcag tatacaacgt tccttgtgct aggaaagaga cgccttcttc
    23041 accagcaagt tttgttgctt cgtcatcaaa aacatatacg aattcattcc caatgatttt
    23101 acgtttttgt tctgggtcag atacaccttt caacttgtct aagaagcgat ctcttgcatt
    23161 gactttgatg atgttcaagc cgaattttcc gcctagactt tccattactt gttccgcttc
    23221 gcctttgcgt agcagaccgt ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt
    23281 ttgtaaaaga accccaacga cactagaatc aacgccacct gaaagaccaa gcaagacttt
    23341 tttgtcacca acttgttcac ggattttttc tacttgcata tcgataaagt tatccattgt
    23401 ccagttacct tcacagtgac aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta
    23461 ttcggaatga cgtacttctg catggaattg gataccataa aaattacgtg ttgtgtcttg
    23521 gatagaagca atcggacaat ctttgcttgt tccgactgtt tcaaagcctt caggaacttt
    23581 agttaccaag tcaccatggc tcatccatac tgtttgttgt tttggtgttt ctttaaacaa
    23641 gacagcttga tcgcttgtta cttctaacaa ggctttccca tattcgcggt ttttagcagg
    23701 ctctacggaa ccgccaagtt tgtacgtgat caattgcata ccatagcaaa tccctaatac
    23761 aggaatacct aaattgaaga tctcaggatc gatcccaaat gcattctcat catatacact
    23821 gtttggtccg ccagaaagaa tgattccctt tggattcatc gcttttactt cttctgcagt
    23881 aatgcggtgg ctcaataatt cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa
    23941 ttggttatac tgactaccgt agtctaatac gataatcttt tcaacgttcg tcatatcggc
    24001 aacgtttgtc aaatttatta cccctatccc tttaaatttt atctcaaaat agtcaaacta
    24061 ttttaaaatg ctaatatttt cgcatgaaaa tcgaatcgat gatatgattt tcagttttat
    24121 ggagaattat atcggctctt cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat
    24181 tcacattttt ccatacatct tttgccattt taaaggcttc ttcacgatct ccgatagcaa
    24241 aacgataata ataattctct ggattttgga aagccgtatc cagtaacgca ccaaaacgat
    24301 ccaaatacca tttttcgatc agcacgggat cggcatcgac atagacagaa aaatcaaaaa
    24361 agtcgctgac gtagatttgc tgattcgcag gtaattgaag cgtattgatc ccctcaacga
    24421 tcaagatatc aggctgctgg atcaattcgt acttgtctgg aatgatatca tacacatcat
    24481 gtgagtaaac aggtactttt atctcttcct gaccagattt tacacggttc aagaaatcga
    24541 tcagcatctc catatcataa ctttcaggaa atcctttacg gtccatgatt ccacgctctt
    24601 ttaatatacg atttggataa agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt
    24661 tgaaggtacg agacaaaatc gtctgcagca gtctggctgt tgtacttttt ccaacggcta
    24721 cacttccggc gatcccgata ataaaaggcg gtacttctac gtatttatga agaaataaac
    24781 ctttactcaa gaacaaagat tcaaattctt tcatataaag ataaatcaga tgagcaagag
    24841 gtacatagat atctcgaaca tcctgcatgg aaatgcggtc attcaaactt ttgatattat
    24901 ctagttcatc ttgtgtcaaa ggtgcttgcc cattacgata aaattctcgc cattcttctc
    24961 ttgccacacg gtagtagttc attggatcat tcatattagc ctcctgcgtt ctcccatcaa
    25021 tggaactatt ttaacacaaa ataaacgcaa accccaccac ttaagagaga ttgccgatct
    25081 ttttgtataa tctttataat agaaagaaac ggagtcttat tcttgacttg cgcacggata
    25141 cataaaagga tagactgttg tcatgaaaga aatgagaagg atggaagaca tgacaaatca
    25201 ttactattct gaacgcccag atacggcgca cgattttgaa caatgggctt ttgagttaaa
    25261 aggcagaacc tttcaatttg taacagattc tggtgtattt tcacgtgaaa cagtggatta
    25321 tggttcacgt gtcctgattg atgctttcga ttgggaagag ttacctgatg aaggaaagat
    25381 cttagatgtg ggatgcgggt atggaccgat tgggttagct ttggcatttg cgacacagcg
    25441 gtttgttgaa atggtcgaca ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa
    25501 gcgtaaccaa atcgaacatg ttgatatcca tcaatcgaat atctatgaag cagttcatga
    25561 agaaacctat gcagctatcg taagcaatcc gccgatccgt gctggaaaaa aagttgtaca
    25621 tgaaattttg acaggcgcat atcctcggtt gaagaagggc ggtacactga caattgtgat
    25681 ccagaagaaa caaggagcgc cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc
    25741 agaaatcgtc acaaaagata aaggatatta tatcatcaaa agtgtgaaag aataactagc
    25801 tgaaaaatag tggagcgacg aaagagtgga actgggcgtg catgtacggg tacgtatcag
    25861 ttcttttttt gttgagaagt ttttaaaaac aaagaactat tttttcttca aaaagaaata
    25921 gtaggaacag agtcgttgtt ttttacgata aaataaataa gatacagaac ttgtatagaa
    25981 ggctgccaag tcggtagata aaggaggttt ttatcaaaaa acatactttc cttttttctc
    26041 tattcttttc ttgggggttt tggtacactg atagggaatt tagatagaag ggagccaata
    26101 ggatgaagct gaccatcaga gatatcgcag aaatggcagg cgtatcagta acaactgtct
    26161 cacagatttt gaataacaaa ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt
    26221 cgatcgtgaa tgaacatcat tataagcccg attatttcgc ttcaaatgtg atcaatcgac
    26281 attcaaagac catcggaatg atcgtccctg atgtcactga tttcttcttc tccaaactga
    26341 tcgaaggtgt agagagctat ttgaatcctt tgggctatgt gatcatgtta tgcaattctc
    26401 gtcatagcca agaaaatgag atcaagtatt tacaagagct ttcccaccgc tcagtggacg
    26461 gaatcttgct ggccacacca aatattttac ctgagaaata tgcattagac agcggttttt
    26521 atcaaaaaat gccaatcatt ttaatcgatc gcggattgaa tcgtcgggat aacggacggc
    26581 ttatcgtaaa ggaatatgag ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc
    26641 atacgaagat cggcatgctg aaggaaacca ctggatacta tcaattagaa gaacgtttca
    26701 acggatatcg tcatgcgctg aaagatcatg gcctgccttt taatgggaaa tttgttgaac
    26761 atggagagtt gactgtccaa ggtggatata ctgcatcaaa aaaattactc aaacataaag
    26821 atatcacggc tattttttgc gggaatgatg ccatggcgat cggatgctat caggcgatcg
    26881 atgaattggg caaaaaaatc ccagaagata tctcagtcat aggattcgat ggattaaagc
    26941 tgtcagaata catgattccg caattgacga cagtgcagca gccaagtttt gatattggat
    27001 tttatgctgc acgtttcctg atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca
    27061 aagtgttcga aacaaaatta attattcgtg agagcgtaaa agtattgaca aagggctaac
    27121 tcggcggtat attgcttatg taagcattta caaaggctag ttagtgcttt ttttattttt
    27181 agtaaaccag tttactaaag tggtttacct ttcctaaaaa taatgtataa taccgaatga
    27241 agacaaggag gatcaggcaa tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa
    27301 cgagctgtcg aaagaggaaa tcgagtacat cgtcacgaac tatacgaatg gaaagattcc
    27361 agattatcaa gtcagtgcat tattgatggc aattttttat caagacatga caaatgaaga
    27421 aatcacgaac ttaacattgg cgattgctaa ttctggtgat gtcatcgatt tgagttctct
    27481 tgaaggaata aaggtagata agcattcgac aggtggcgtt ggtgacacga ccacattgat
    27541 tttagccccc ctagtagcta gtgtcggagt gacagtggct aaaatgtctg gacgcggtct
    27601 aggttacaca ggtggtacac tagacaagct agaagcaatt cccggatttc agatcgagct
    27661 ttcggatgaa gaatttgtac gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc
    27721 aggaaacttg gcaccggcgg acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt
    27781 tgattctctt ccgctgattg ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga
    27841 tgcaatcgtt ttggatgtga ccactggaga cggtgctttc atgaagaaca tcgaagatgc
    27901 aagacgtctg gcaaagacca tgacgagcat tgggaaactg gctaaccgag aaacagtggc
    27961 ggttatctca gatatgtcag aacctcttgg cgaagctatc ggaaatagtc tagaagttgt
    28021 cgaggcaatc gaaacattac aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc
    28081 cttaggaagc caaatggttg ttcttgctgg caaagctaag acaaccgatg aagcaagaac
    28141 gttgcttcaa gaagctctgg aatcaggtaa agcgttagca aaattcaaag aaatgatcca
    28201 aaatcaaggg ggagacccag cgatcgtgga acatccagaa cgaattttga ctgcacgcta
    28261 cacaatggag cttcctgcta aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt
    28321 aggaattgct gcaatgatgc taggtgctgg gcgtaaaacc aaagaagaag atattgatca
    28381 cgctgttgga ttgaaactgc ataaaaaaat tggtgacact gtgacaaaag gtgagtcact
    28441 attgacgatc tatagtaacg acgaagaaat cacttctgtc attgaattat tatataaaaa
    28501 tattgaaatt ggtgaatcgg caatgaagcc aacgttgatt catgacatca tcacagaata
    28561 aaggaaaaaa gcggacttta ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg
    28621 aatgatcgac catactattt tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat
    28681 cgaagaagca aaaaaatacc atttttattc tgtctgcatc aatccagcat gggttgcgct
    28741 agcgaaagaa caattacaag gtgagccagt agccgtatgt acagtaatcg ggtttcctct
    28801 aggagcaaac acacccgaga caaaagcatt tgaaacaaca gatgccatca ataacggtgc
    28861 agatgaagtc gatatggtca tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt
    28921 ccaaaaagat atcgaagcag ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat
    28981 tattgaaaca tcgttattag atagagaaga aatcatccga gcttgcgaaa tagcaaaagc
    29041 tgcaggcgct gattttgtca aaacatcaac tggtttctca acaggcggag caaaggtaga
    29101 ggatgtccgt ttgatgcgtg aaactgtagg accagaaatg ggtgtcaaag cttccggcgg
    29161 tatccataac gaagaagaag caatggcaat gatcgaagct ggtgctacaa gaatcggaac
    29221 aagcgcagga gtagcaatcg tatctggttc aactggtgaa ggctattaat tttttcaaag
    29281 gagaagctca tggcagaact caaacaagaa tggatcaaca cagctattga agcgttagat
    29341 aaagcctatg ttccttattc ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc
    29401 aagatttatc aaggcatcaa cattgagaat gcatcatttg gattaacgaa ttgtgccgag
    29461 cgcacagctt ttttcaaagc agtatctgaa ggagaaaaaa catttacgca tctagtcgta
    29521 gcggggcaca cccccgatcc tatctctcct tgtggtgcat gtcgacaagt aatggcagaa
    29581 ttctgttccc ctgatatgcc ggttacgcta gtaggagaca atggtgtgac gaaggcaacg
    29641 accgttcgtg cactgctgcc ttatgcattt actgaaaaag atctataact catgttcatt
    29701 cagtcagctg actgttgaaa aataaaacaa ttattttagg aggctttaca gaaatgaaaa
    29761 aagcaaaatt atttggttta ggtgctgtcg cactagcagc agggttattt ttaggtgcat
    29821 gcggcaacaa cggctctact gattcatccg gtggcaaaag ctcatcagac acaacaactg
    29881 cagcattgat cacagacaca ggtggtgtag acgaccgttc attcaaccag tcagcttggg
    29941 aaggactaga aaaatggggg aaagatcaag gactttcacg aggaaatgat ggcttccaat
    30001 atttccaatc ttcaaatgaa tcagattaca tcccaaatat tgaccaagca ttgaatgctg
    30061 gattcaaaac gatttttggt attggttata aactaaaacc agcgatccaa gaacaagcaa
    30121 caaacaatac aggtacgaac tttgttatta tcgatgatgt catcgatgga ttagataacg
    30181 ttgtatctgc aactttcaaa gacaacgaag cttcttatct tgcaggtgtt gcagcagctt
    30241 atacgactga aacaaacgta gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc
    30301 gttttgatgc tggattcaaa gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa
    30361 tcaaagtatt gaatcaatat gctggtgact tctctgctcc agataaagga cgttcaattg
    30421 ctcaaggtat gtatgcacaa aacgcagata tcatcttcca tgcttcaggc ggtacaggta
    30481 acggtgtatt ccaagaagca aaatcattga acgaatcagg cgacaaaaaa gtttgggtta
    30541 tcggtgttga ccgcgaccaa tcagacgaag gcgaatacac attaaacggc gaaaagaaaa
    30601 acttcacatt gacatctaca ctgaaagcag ttggaacagt agtagaagat ttggcacaaa
    30661 aatcagcgga cggtaaattc cctggcggcg aacacactgt ttatggattg aaagaagacg
    30721 gtgttggtct aacagaagga caactttctg acgaagcgaa aaaagcagtt gatgaagcaa
    30781 aagaaaaaat catttcaggt gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc
    30841 atttctatag ccttgttaga cacatgtttg acaaggctat ggtttgatta gaacaagaat
    30901 tgttcattta tccaagctgc cttgtgacag tttggataag ggaacaactc cctgaagtaa
    30961 tgtttgtttc aaaagaagaa cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga
    31021 tagaaatcgt taggatgtga aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag
    31081 aatattacca aagaatttgg aacattcaag gcaaatgaca acatcaattt acaagtaaag
    31141 gcaggagaaa ttcatgcgtt gcttggagaa aacggtgctg gcaaatctac attgatgaac
    31201 gtgctttccg gattattaga gccgacatca gggaaaattt tgatgcgtgg gaaagaagta
    31261 aagatcacaa gtccgaccaa agccaaccaa ttagggattg ggatggtcca tcagcacttt
    31321 atgcttgttg atgcctttac tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc
    31381 gcagggatgc ttgaccataa aaaagcacga aaagagatcc aaaaagtttc tgaacagtat
    31441 ggattgtcag tcaacccaga tgcttacgtt cgtgatattt cagttggaat ggaacaacgg
    31501 gtagaaattt taaaaacact ttaccgagga gcagatgtac tgatttttga tgagccgaca
    31561 gctgtattga cccctcaaga aattgatgaa ttaatcgtga tcatgaagga attagtcaaa
    31621 gaaggcaagt cgatcatttt gattacgcat aagttagatg aaatcaaagc agtagctgac
    31681 cgctgtacag ttatccgccg tggaaaagga atcggtacag tcaacgttaa agacgtaacc
    31741 tcacaacaat tagccgatat gatggttgga agaacggttt cattcaaaac gatgaaaaaa
    31801 gaagcgaagc ctcaagaagt cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt
    31861 ggattagaag ccgtgaaaaa cttgaactta gaggttcgtg ctggcgaagt acttggtatc
    31921 gctggaatcg atggaaacgg gcagtcggag ttgatccaag ctttgactgg tttgcgaaag
    31981 gcagaaagcg gacatatcaa gctaaaaggg gaagacatca ctaataaaaa accccgaaaa
    32041 atcactgaac atggtgtagg acacgtgcca gaagaccgtc ataaatacgg gttagtccta
    32101 gatatgacat tatctgaaaa tatcgcacta caaacctatc atcaaaaacc ttacagtaaa
    32161 aacggcatgc tgaattattc agtaatgaat gaacatgcca gagaattgat cgaagaatat
    32221 gatgttcgaa caacgaatga acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa
    32281 aaagcaatta tcgctcgtga agtcgaccga gatcctgatc tgttgattgt tgcaaatcca
    32341 actcgtggac tggatgtagg tgcgatcgaa tttattcata aacgtctgat cgaacaaagg
    32401 gacaaataca aagcagtgtt actgatcagt ttcgaattag aagaaatttt aaatgtttcg
    32461 gatcgtattg ctgttatcca tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca
    32521 tctgaaaatg aattaggtct attgatggcg ggctatacct tagaagaagc acgaaaagag
    32581 ttagaaaagg gggctggcgc acatgaatga tcgcagcgaa aaattccgta atttattagt
    32641 gcctgtactt tcagttgttt taggattgat cttaggcgcg atattgatgg ctgcttttgg
    32701 ttttcagccc atccaaggtt attcttctat gctagatgca gcccttggtt cgcaaagagg
    32761 gataggtgaa acgttaaggc aagcaacgcc gttgattttt acagcattag gtttttctgt
    32821 agctaactca gctgggtttt tcaatattgg gctttctgga caagcacttt gcgggtggat
    32881 cgtcagtgtc tgggtggcac ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt
    32941 atgcgtgatc ttaggtgcct tagctggtgc gatggcagcg gctgttccag gtttacttcg
    33001 agcatttttt ggaacaagtg aagtgatcgt aacaatcatg atgaactata tcttattata
    33061 cgtaagcaca tttgctttac atgaagtgat gcctgaaagc tatcgctcaa gtttggactc
    33121 ttccaatatg atcagtcaaa atgcctcact gcggttacca tggttaacac aagcttttgg
    33181 cggctctcgc gtaaactcag gattattttt agcaatcatc gcgcttattt tcgtttggct
    33241 gctgatgaag aaaacgactt taggatttga aatccgttct gttggattga atccttttgc
    33301 ttctgagtat gcaggaatga gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg
    33361 cgctttggca gggcttggcg gtgtggttga aggattagga acgtatcaaa acttctttgt
    33421 tcaaacgact tcgctatcta ttggatttga cggaatggct gtttccttgc ttggttctgg
    33481 atcagcaatc ggtattcttt tatcagcgat cttgttcagt attttgaaga tcggtggact
    33541 tggcatgcag acaggagcag gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat
    33601 tatcttcttt gtcgggatca acttcctgat tcgtttcgtc atggctaaag tcttccaagg
    33661 gaaaaaacaa gaagagatcg ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga
    33721 agggggcgaa ctataatgtc gactatcgaa ttaattgcct tgatccttac ttctacactg
    33781 gtttattcaa caccgctaat tctaacgtca ctaggcggaa ctttttctga acgaagcggg
    33841 atcgtaaacg tagggctaga gggaatcatg gtcatgggtg cttttagttc catcgttttc
    33901 aacttgacga tgagtccatc acttggttct atgacaccat ggatcgctat tttagtgggt
    33961 ggcgtcgtag gtatcctatt ttctctgatt cacgcagtag caacagtcag tttgcgtgct
    34021 gaccatatta tcagcgggac agtaattaat ctgatggcac ctgctttagg tgttttcttg
    34081 atcaaagcat ggtatggtaa aggtcaaact gataacatca cgcagaatct ggggtatttt
    34141 tctttcccag gattagcaga tattccggtc attggaccaa ttttctttaa aaatacattc
    34201 ttacctgctt attgtgctat tttgattgct gtcctagctt gggtgatcat cttcaagacg
    34261 aaattcggct tgcgcctccg ttcagttggt gaaaatcctc aagctgccga cacattaggt
    34321 atcaatgtat atgctatgcg ttattcaggc gtactgattt ctggcttgtt aggcggtatg
    34381 ggcggtgctg tttttgctca gtcgatttcc ggtaactttt cagctggtac gatcgttgga
    34441 caagggttca tctccatggc tgctatgatc tttggtaagt ggaatccttt aggggcaatg
    34501 ggtgcctcat tgttctttgg ttttgctcaa agcttgagta tcattggcgc acaattacca
    34561 gttatttctt cgattccgcc agtattgctg caaattgctc catatttgct gacaatcatt
    34621 gtgctggttg ttttcttagg gaaagcttct ggtccaaaag caaacgggaa gaactatatc
    34681 aaatcaaaat aatcttttag cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc
    34741 gccctttttg ttttaaaggc aataaaaata aaaagaaaga aggaataatc atgtttaaac
    34801 gcgtacatct gatcgtcatg gattcagtag gaattggaga agcaccagat gccgataagt
    34861 tcggtgatgt tggttctgat acattaggac acattgcgaa agaagcagga ttgaacgttc
    34921 cacatttaga gcagcttggt ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga
    34981 aagatcatca aggctatgcg acgaaattag aagaagtttc tgtagggaaa gatacgatga
    35041 caggccactg ggaaatcatg ggattgaata tcaaaactcc atttcgtgtg tttcctaatg
    35101 gattccctga tgatctgatc aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta
    35161 acaaacctta tagcggaaca gctgttattg acgattacgg tgaacaccaa atgaaaacag
    35221 gcgatttaat tgtatatact tctgccgatc ctgtccttca gatcgcggca catgaagaaa
    35281 tcattccttt agaagaatta tatcggattt gccaattcgt tcgtgatata acaaaagatg
    35341 atccgtatat gattggccga atcattgcac gtccgtacgt gggtgaacca ggagacttca
    35401 cacgaacaag caatcgccat gattatgcgt tgaatccatt cggacacaca gtccttgatt
    35461 ctttgaaaga agcaggaaag gacgtgattg ctgttggaaa gatcaacgat attttcaacg
    35521 gacaaggaat cactgaatct gtccgcacga aaagcaatat ggatggcgtt gaccagctgc
    35581 tgaaagttat gaaaaacgat ttttcaggaa tcagctttac caatttagta gattttgatg
    35641 cattatacgg tcatcgtcgg gatgtagtcg gctatgcgca tgcgattgaa gaatttgact
    35701 tacgaattcc agaattgctt gaagcgatgg aagaagacga cttgctaatg attacagccg
    35761 atcacggaaa cgatccgacg tttactggta ccgatcatac gcgagaatat gtaccgttat
    35821 tggtttacag taaaaaaatg aaagaacaag gggcattgcc tcaaggatat tattcagata
    35881 tttcagcaac gattgctgag aactttagtg tttcttcgac tgaaaatggc gaaagcttct
    35941 tgaaattatt aaaataatct ttttaaatag gagaaagcaa atgaccaagt taagcgagat
    36001 gttacaagaa acgacccagt ttatccttga taaaggagta aaagaaattg attttggttt
    36061 gattctaggt tcaggattag gtgaattagc agatgaaatc gaagatgctg taatgatttc
    36121 ttatgatgag atcccgttct ttcctgtttc gacagtagtt gggcatgcag gaaagctagt
    36181 ttatgggtct ctggcaggca agaaagtgtt agccatgcaa gggagattcc actactatga
    36241 aggtcattcc atgcaaacag tgacctatcc agtacgtgtg atggccggac taggggccca
    36301 ttcagtcatt gtgactaatg catgtggtgg tgtcaacgaa tcatttgtac caggagattt
    36361 gatgctgatt accgatcata tcaattttac tggtgaaaat cctttgatcg gtccgaatga
    36421 agatgcaatg ggccctagat ttccagatat gagtcaagct tatacgcctg actatcaagc
    36481 aacagcaaaa gcagcagcag aaaaactagg tttggatctt aaagaaggcg tatatatggg
    36541 ctattctgga ccaacttatg aaacgcctgc ggagatccga atgtctcgga caataggagc
    36601 cgatgcagta gggatgtcga ctgttccaga agtcatcgta gcagctcaca gcggattaaa
    36661 agtgttagga atctcttgta ttaccaactt agcagcaggt atgcaagcga atctgagcca
    36721 tgaagaagtt gtagaaacaa ctcagagagt caaacaatct tttaaagcat tggttaaaga
    36781 aacattgaca ttattgtaat aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag
    36841 aaggcgagat cgccgataag attttacttc cgggagatcc tttgcgagct aaatatattg
    36901 cggagacttt tttggaagat ccagtttgtt acaatcaggt acgcggtatg ttaggcttta
    36961 ccggcaaata taaaggagaa cgtatttctg ttcaaggaac aggaatggga atgccatcag
    37021 ccaccatcta tgcccatgaa ttgatccagt cgtatggtgt aaagaaattg attcgagtag
    37081 gtacatgcgg tgcattgtca aaagacgtcc atgttcgtga tttagtacta gcacaaggtg
    37141 cagcgaccag ttcttcgatg atcgaaaaaa atttccaagc ctttcatttt cctccaatca
    37201 gtgatttcaa tctattactg aaagcatatg agatagctaa agaaaagggc tatacggttc
    37261 atgtaggaaa tgttctttcg gaagattctt tctacaaaga tgatttgaca gagacgttcc
    37321 aattagctga acttggcgtt ttaggagtgg aaatggaagc agcggctctt tattacttag
    37381 gcgcgaaata ccatgtgcag acattaagtt tgatgaccgt tagcgaccat ttgatcaccg
    37441 gtgaagaaac aacagctgca gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat
    37501 tggaaacagc gatagcagaa taagaaggaa atgaaaaagc tgccctgcat taagtaataa
    37561 gaacatccaa gcaaaaatag ctcctcatgt ttttgacttg gctggactta ttattgcaaa
    37621 tgtaagcttt tgaacaccgt ttaccaagag gttgtgaatc tgccgtttaa ctctgagtca
    37681 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    37741 gaagttggca ggagaaaacc gtttatacga aaaaagggac tgtgacaaat cgtgtcacag
    37801 tccctttttt atagtgt
//
LOCUS       DAYYGS010000017        37620 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000017 DAYYGS010000000
VERSION     DAYYGS010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.017"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..120
                     /locus_tag="ACHA44_000824"
     CDS             <1..120
                     /locus_tag="ACHA44_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8904136.1"
                     /translation="QGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA"
     gene            complement(270..704)
                     /locus_tag="ACHA44_000825"
     CDS             complement(270..704)
                     /locus_tag="ACHA44_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8904137.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(804..1652)
                     /locus_tag="ACHA44_000826"
     CDS             complement(804..1652)
                     /locus_tag="ACHA44_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8904138.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            2041..3807
                     /locus_tag="ACHA44_000827"
     CDS             2041..3807
                     /locus_tag="ACHA44_000827"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFM8904139.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            3909..5168
                     /locus_tag="ACHA44_000828"
     CDS             3909..5168
                     /locus_tag="ACHA44_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8904140.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            5315..6616
                     /locus_tag="ACHA44_000829"
     CDS             5315..6616
                     /locus_tag="ACHA44_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904141.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            6619..7470
                     /locus_tag="ACHA44_000830"
     CDS             6619..7470
                     /locus_tag="ACHA44_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFM8904142.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            7483..8298
                     /locus_tag="ACHA44_000831"
     CDS             7483..8298
                     /locus_tag="ACHA44_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HFM8904143.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            8449..9615
                     /locus_tag="ACHA44_000832"
     CDS             8449..9615
                     /locus_tag="ACHA44_000832"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8904144.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            9845..10492
                     /locus_tag="ACHA44_000833"
     CDS             9845..10492
                     /locus_tag="ACHA44_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFM8904145.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            10576..11520
                     /locus_tag="ACHA44_000834"
     CDS             10576..11520
                     /locus_tag="ACHA44_000834"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HFM8904146.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            11655..13106
                     /locus_tag="ACHA44_000835"
     CDS             11655..13106
                     /locus_tag="ACHA44_000835"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HFM8904147.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            13127..13963
                     /locus_tag="ACHA44_000836"
     CDS             13127..13963
                     /locus_tag="ACHA44_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8904148.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            13963..14622
                     /locus_tag="ACHA44_000837"
     CDS             13963..14622
                     /locus_tag="ACHA44_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8904149.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            14748..15437
                     /locus_tag="ACHA44_000838"
     CDS             14748..15437
                     /locus_tag="ACHA44_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904150.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            15443..16399
                     /locus_tag="ACHA44_000839"
     CDS             15443..16399
                     /locus_tag="ACHA44_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HFM8904151.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            16443..16955
                     /locus_tag="ACHA44_000840"
     CDS             16443..16955
                     /locus_tag="ACHA44_000840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904152.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(17211..17759)
                     /locus_tag="ACHA44_000841"
     CDS             complement(17211..17759)
                     /locus_tag="ACHA44_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFM8904153.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(17848..19029)
                     /locus_tag="ACHA44_000842"
     CDS             complement(17848..19029)
                     /locus_tag="ACHA44_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HFM8904154.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(19265..20773)
                     /locus_tag="ACHA44_000843"
     CDS             complement(19265..20773)
                     /locus_tag="ACHA44_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HFM8904155.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            20932..21123
                     /locus_tag="ACHA44_000844"
     CDS             20932..21123
                     /locus_tag="ACHA44_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904156.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            21326..22243
                     /locus_tag="ACHA44_000845"
     CDS             21326..22243
                     /locus_tag="ACHA44_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HFM8904157.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            22236..23309
                     /locus_tag="ACHA44_000846"
     CDS             22236..23309
                     /locus_tag="ACHA44_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904158.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            23457..23975
                     /locus_tag="ACHA44_000847"
     CDS             23457..23975
                     /locus_tag="ACHA44_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904159.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            23956..24348
                     /locus_tag="ACHA44_000848"
     CDS             23956..24348
                     /locus_tag="ACHA44_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HFM8904160.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            24424..25359
                     /locus_tag="ACHA44_000849"
     CDS             24424..25359
                     /locus_tag="ACHA44_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8904161.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            25492..26271
                     /locus_tag="ACHA44_000850"
     CDS             25492..26271
                     /locus_tag="ACHA44_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8904162.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            26264..26581
                     /locus_tag="ACHA44_000851"
     CDS             26264..26581
                     /locus_tag="ACHA44_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8904163.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            26825..28435
                     /locus_tag="ACHA44_000852"
     CDS             26825..28435
                     /locus_tag="ACHA44_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8904164.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            28592..29440
                     /locus_tag="ACHA44_000853"
     CDS             28592..29440
                     /locus_tag="ACHA44_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8904165.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            29671..30150
                     /locus_tag="ACHA44_000854"
     CDS             29671..30150
                     /locus_tag="ACHA44_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="HFM8904166.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            30183..32411
                     /locus_tag="ACHA44_000855"
     CDS             30183..32411
                     /locus_tag="ACHA44_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8904167.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            32540..34183
                     /locus_tag="ACHA44_000856"
     CDS             32540..34183
                     /locus_tag="ACHA44_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFM8904168.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34256..34801)
                     /locus_tag="ACHA44_000857"
     CDS             complement(34256..34801)
                     /locus_tag="ACHA44_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HFM8904169.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(34824..35309)
                     /locus_tag="ACHA44_000858"
     CDS             complement(34824..35309)
                     /locus_tag="ACHA44_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8904170.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(35299..36111)
                     /locus_tag="ACHA44_000859"
     CDS             complement(35299..36111)
                     /locus_tag="ACHA44_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HFM8904171.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(36250..37362)
                     /locus_tag="ACHA44_000860"
                     /pseudo
     CDS             complement(36250..37362)
                     /locus_tag="ACHA44_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
     gene            complement(37383..>37620)
                     /locus_tag="ACHA44_000861"
     CDS             complement(37383..>37620)
                     /locus_tag="ACHA44_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8904172.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT        12351 a         6792 c         7419 g        11058 t
ORIGIN      
        1 caaggattca tctcatccgt tgcgtccaaa agaaaccata tccctagaaa atcactaaat
       61 taccaaacac cattagaagt ttttttgagt tacgtaaatg gaaagttttg tctcgcttaa
      121 tttgacaaat aatacaatat aattgtggaa agcaaagaaa tggattggca taagataaat
      181 aagagaaagc acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc
      241 agagacaaaa aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt
      301 tcttcaaaaa ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct
      361 ctgaacattg agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca
      421 cagccatttc gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac
      481 cagatcgtat ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc
      541 agcatatgga atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa
      601 tctgttgcct caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc
      661 aaactatcat ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct
      721 tttcagtcga acagttacta gattatttta ttataccagc taaagaaaac gcattcaaaa
      781 aaatagcgga tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt
      841 acttctgcta cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt
      901 ttcattcggg aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga
      961 ttatacccgc cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga
     1021 aggcggatgt cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata
     1081 cctgtttcga tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga
     1141 aactcttcta ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat
     1201 aagccaatcg aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta
     1261 ccgccgctag aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt
     1321 ccaaaaatga cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg
     1381 attaaaggaa aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat
     1441 agaagagcta gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag
     1501 cctaaagcag cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga
     1561 ggccctaaaa acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg
     1621 tatgtccata taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat
     1681 actcttatct tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt
     1741 aggctagtct aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt
     1801 taaaaaactt ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac
     1861 taaatagtac aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag
     1921 tttaaaaaat aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg
     1981 tcttttgctt ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca
     2041 atggatacag ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat
     2101 acaagatttc gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta
     2161 tgtggagatc catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa
     2221 aaatgtctaa gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt
     2281 cgcttacagt atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat
     2341 caaggaattt ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata
     2401 ccttattttc atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg
     2461 tatcaaattt ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact
     2521 ttaccttggg gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt
     2581 gtgatggatc atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc
     2641 atatttaaag ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc
     2701 gcttttggcg ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata
     2761 cgtatcatgt tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac
     2821 gttctcaaaa acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca
     2881 gtagattctt atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact
     2941 tttgctttta ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat
     3001 ttgctggata tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat
     3061 gttgctaatg aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc
     3121 aaaccagaca tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg
     3181 gatgaattcc atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca
     3241 aaaaagaaaa tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac
     3301 agagatcagg tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc
     3361 ttgacccttt gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt
     3421 caaaaagggt ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac
     3481 ccggattgcc gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt
     3541 tttataaaag aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct
     3601 actcagtggc taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag
     3661 ggacagatca tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa
     3721 aatgaggtga ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga
     3781 caatacggat ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg
     3841 caaatgaaga tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg
     3901 aaatgaagat gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa
     3961 tcttaggcgg attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg
     4021 aaggaaaaac gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa
     4081 aaaacacttt cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg
     4141 atcagttgga atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg
     4201 cagcatatga ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg
     4261 gaaatgaagc agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct
     4321 acggagaacc tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata
     4381 cagcaccatc aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg
     4441 agtcagaagc aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct
     4501 atggtttaat tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag
     4561 atatcggttt gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca
     4621 aaaatgtttg gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc
     4681 aattcatgtc caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca
     4741 aagaaaacaa tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt
     4801 atcaaccgtt tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc
     4861 tctctcaaaa atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa
     4921 tggtaaatga aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg
     4981 aattaacaac agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag
     5041 aaatgggcga agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa
     5101 aaacaccaaa agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa
     5161 aatactgatt aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata
     5221 aaagtggcaa gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag
     5281 tttgcggttt agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat
     5341 caacgaaaaa aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg
     5401 ccaagctttt aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct
     5461 tctgggcgga tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga
     5521 tcattcatta tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt
     5581 gattttttac ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg
     5641 caaaaaaatc aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc
     5701 ttatctattg acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct
     5761 agttacattg ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt
     5821 aattgattgg gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa
     5881 tacttttttt gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca
     5941 aatcaccctt ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg
     6001 tattttcggt atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag
     6061 tttttcgaat atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat
     6121 aaaccagctt ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac
     6181 gaaagtagca attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt
     6241 tacaggaatc ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc
     6301 aaatgcatgg cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc
     6361 aacatttatt acacagtata cggggaattt caataatttc tccatgatct atctgtttaa
     6421 caatggcggt cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg
     6481 gatctataaa cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt
     6541 gattatctcc gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt
     6601 caatatgggg gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt
     6661 tctcactttt ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt
     6721 ttgatcacag caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt
     6781 tcaagtgact ggactttagg aaactttact cggctattca gtgaaacact ttacgggact
     6841 tggtataaaa atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta
     6901 actttggcgg gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg
     6961 ttctttttag tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg
     7021 gctttcttat tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt
     7081 ggaattccaa tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta
     7141 gatgaatctg ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta
     7201 ccgcttgtca aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg
     7261 gatttcatgt tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc
     7321 ttacaaacat ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca
     7381 attttgattg cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc
     7441 ggcctgacat ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta
     7501 aactattttt ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca
     7561 tggcctaaat ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact
     7621 cttttttatg ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg
     7681 ctgatcgatg aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta
     7741 caagcatcgc aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct
     7801 gagaaacaac aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc
     7861 caacaaaagg ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa
     7921 ccagattctg tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc
     7981 agtaatcgtc caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc
     8041 atgacgagtc tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa
     8101 ctttcatcca ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata
     8161 ggttccatgg cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt
     8221 gtccaaacaa cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa
     8281 gataagcgtg tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg
     8341 aaaaataaag tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt
     8401 cttttttagt attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa
     8461 actttataca atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg
     8521 gacggtacaa cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc
     8581 catgaagaaa tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc
     8641 tatatggatc atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat
     8701 cagcaagcaa acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa
     8761 aaacatacga aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac
     8821 ttttataaac tttggctaga tccaactttg acttattctt gtgcttattt caatacacct
     8881 gaagatacgt tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt
     8941 atcaaagaag gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact
     9001 gctaccaaag agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat
     9061 ttgattacca aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg
     9121 gattaccgcg aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa
     9181 catgtaggtt ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca
     9241 aaaggaacag ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg
     9301 atcaataaat atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat
     9361 attacaaaaa acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg
     9421 acattagaac attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg
     9481 ggtgattcct tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa
     9541 tcaagtaata tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca
     9601 atgcatcgtg cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag
     9661 ttttatgatg ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt
     9721 agtttctttt tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac
     9781 agaaaatttt ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga
     9841 atcaatgaaa aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg
     9901 tcaatcaaat accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc
     9961 ttcacaggta tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag
    10021 ttctagtgaa gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt
    10081 gttccaagat aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg
    10141 atcttacttc tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga
    10201 tgctcaaaat gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa
    10261 tggtcaaaag aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc
    10321 agcaaaaatc gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa
    10381 agatttaggt actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt
    10441 caagatcaat agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct
    10501 tcggaaaaca agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt
    10561 gaaggagcga tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct
    10621 ttgatgaatg catctggtgt ccactgcatg actacccagg aattagatga actggctcat
    10681 tcagaagcag gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc
    10741 gaaccacgct attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta
    10801 ggtttttctt attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat
    10861 caaccgttat ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag
    10921 aaaatagaaa aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg
    10981 cctggaaaac cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta
    11041 ttcagcatct tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat
    11101 tttgatcaga tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac
    11161 agtgtgggca atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa
    11221 gaagggttcg gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc
    11281 gctttttata cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca
    11341 ggacaagatg catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtacagag
    11401 cttcataaag aagggccgga aattttttct agaatcataa aagaattaac gcagattatg
    11461 tcagaaaaag gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag
    11521 caaagagacg aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc
    11581 attcctgtgc aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca
    11641 aaaaggagga taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat
    11701 ttagttttca agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat
    11761 tgaatgcgtt tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc
    11821 agtcagaaaa agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa
    11881 aaaagggatg gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata
    11941 catctaatta tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg
    12001 cgccagagtt tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc
    12061 cttggaacct tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat
    12121 caggaatcgt tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt
    12181 cgttttgcgg attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg
    12241 cttggagagg atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca
    12301 cggaggcttt attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc
    12361 cagttgagtg gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt
    12421 caccgatcca ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt
    12481 tccttggaaa agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac
    12541 tgatcacgtc ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg
    12601 aaacaggcat ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt
    12661 tccaatatgg tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg
    12721 aagcagcaat tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga
    12781 ctgcatttac cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga
    12841 tggaacacat cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg
    12901 aggagagctt gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa
    12961 tcagcttgcc tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg
    13021 ccagaggaag agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt
    13081 tccatttacc aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag
    13141 attgtttgct ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc
    13201 taatcaggaa gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac
    13261 ttctggcaca ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga
    13321 tgtagatttg gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata
    13381 caacgtggat ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga
    13441 ttttggtatc acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc
    13501 atttgacagt atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct
    13561 taaagggaaa aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca
    13621 cggagctaaa ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc
    13681 aaatggtcga acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc
    13741 tttaccagat atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg
    13801 ttttttattg aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga
    13861 gatgaaagaa gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc
    13921 agtcaaaccg aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt
    13981 gatttttccc tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta
    14041 gggattggct tcgtgtcgtt tcttttagga aatttggtag ggttgctttt gacagtttta
    14101 ggtcttttgg attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga
    14161 ggcattcccg cactagtcct gctgtttctg ttatatttcg ggttacctta tcaattaagt
    14221 gccttaacgg cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc
    14281 tatcgaggat cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt
    14341 ttttctttct ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta
    14401 ccggcactca gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc
    14461 acagtgccag aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt
    14521 tctctctata ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg
    14581 caacagtatt tagaaaaacg ttctatccat cagtcaatct gaaaaataga aatggtgcta
    14641 gcgtggctgg caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac
    14701 catttcatgt aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc
    14761 taaccatcga aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg
    14821 tagaagaagt cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa
    14881 aatttgatgg aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc
    14941 gctctacagc caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac
    15001 tatatgaaga agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt
    15061 acacggttga agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa
    15121 agaaagagct tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca
    15181 gggaagtaag aaaagcaaag caaatggagc gaggcgtaaa atattttcca aaagatcaat
    15241 tgtacaaaga attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt
    15301 atgaacggtt tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta
    15361 tcgcgcttca gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta
    15421 cagagccatt ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct
    15481 attagcatta aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat
    15541 cgaaccagta cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca
    15601 ccatccaatc gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca
    15661 tgtgcacagt tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc
    15721 agaaaatctc agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca
    15781 ctacccaaaa gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat
    15841 accagatact agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc
    15901 ttttcaaacg agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga
    15961 tgactatctt tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg
    16021 tagccgttat ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat
    16081 tgatgcttac ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa
    16141 agaccaagca ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca
    16201 ccatcatcaa ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca
    16261 aaaattccct ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat
    16321 tagtcacctg ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact
    16381 atatgaagtg atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact
    16441 ggatgtatat tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg
    16501 ccgcagcgga tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa
    16561 caaaagaaac gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag
    16621 atcaaattgc tggaacagct gcattagtct caggcgtgga tcccgtttac acaaaaatag
    16681 atggtagttg gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag
    16741 aaagtactcg aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag
    16801 ccagcaaaaa tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa
    16861 agaccatcta ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg
    16921 gcgaaagaaa agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata
    16981 aagacattaa cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt
    17041 ggccagcttc aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt
    17101 ttagcgtatt ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat
    17161 agcttttgtc acgatctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat
    17221 aatccttttt ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa
    17281 agtgagggga tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt
    17341 ctggctgaaa tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct
    17401 ttagagatcg taataaagat aggacggact accgtcccga aatactcata taaatgccaa
    17461 aaaccatttg ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct
    17521 gctttactgg aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc
    17581 ttgatcatct gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc
    17641 ccaggtcttg gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct
    17701 tctgcgagtg aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt
    17761 gtcatttctc ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt
    17821 ttaaaagtaa ctttgctttt ttgtatttta taccagcaag ccagtcacaa tagcacttaa
    17881 aaagctgact aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc
    17941 tttttcttca tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc
    18001 aaatgaaacg aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc
    18061 atttgctaat tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact
    18121 accggcatcc actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat
    18181 gtaacctagg atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat
    18241 ggcaatcaaa gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc
    18301 atcaagaatg tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat
    18361 cagctcatct tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc
    18421 gccaaataag ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata
    18481 agatccgacg atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg
    18541 tttctcagat aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa
    18601 aatagcagaa gctactccat tataagattc caatttgccc atcccgttga ttttacttaa
    18661 tcctagacca acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat
    18721 aatagcagaa ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt
    18781 atttgctaca ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc
    18841 aaacattttt gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa
    18901 accaagaaca agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt
    18961 gttactgctg atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa
    19021 ataactcaaa taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa
    19081 aatactaatc tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca
    19141 ttagaaaata aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta
    19201 taaaaaaacg aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact
    19261 ttttctattg gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc
    19321 ctagtttttt caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg
    19381 ctccctcaag ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac
    19441 tgattgataa agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg
    19501 tctttgattt caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat
    19561 ctgcgatatg ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca
    19621 atgaacttgt acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag
    19681 aaggatcttt tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta
    19741 ccacgtttcg gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa
    19801 ctgcttcttc gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga
    19861 tgatttcttg tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac
    19921 gattcacacc catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa
    19981 taatacgttt gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat
    20041 tgtaggcatc aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg
    20101 cttcataagc aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga
    20161 acttggagta accagctgca tgtccttttt ggctttcatg atacagttgg ttcaagcaag
    20221 tagcgagctt tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt
    20281 caatatactc attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat
    20341 agtcttcgat ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa
    20401 agactttcgt ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc
    20461 cataaccacg taattcatcg attaaccgca agatatccat atcataagta atcccataat
    20521 caccacggat cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt
    20581 gatctttcaa tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac
    20641 gtttcgcatg cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt
    20701 ggttcactcg ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac
    20761 caattttttt cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga
    20821 ttcgtcaact ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt
    20881 gtctagatag ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa
    20941 agacagaaaa agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa
    21001 agaatgcttg aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg
    21061 cagctaactc gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa
    21121 taaaaaataa gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt
    21181 gatcgaaaaa aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct
    21241 cttttttata acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac
    21301 agatgagtaa gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc
    21361 agacgaacta gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta
    21421 cgatatcaaa gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt
    21481 tgaaaaagct gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt
    21541 gaatgaagaa ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg
    21601 taagatccat gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc
    21661 gccaacgatg agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat
    21721 tgatgcaagt ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata
    21781 ttcccaaccg gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat
    21841 cactgctcag acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat
    21901 gactgatttg aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca
    21961 agaattagag aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat
    22021 tgagacgcct gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga
    22081 ccagcttcgt gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag
    22141 caaagaggga ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa
    22201 tcctaataat caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat
    22261 ggacaaaaaa tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac
    22321 aaacaagtaa tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac
    22381 acgaaaaaga aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg
    22441 aaataactga aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca
    22501 gtttgattga aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg
    22561 aatcagattt tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa
    22621 tctccacgat cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt
    22681 tgaatgatat tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt
    22741 tttcgatcaa ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga
    22801 ccatcgatca acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa
    22861 tggaaatcaa tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc
    22921 gagtattctc tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc
    22981 atgcaagaac acttggtttt accgttaatc agacattact agacaatacg aagccacttc
    23041 gtttgaatct tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg
    23101 tggcaatgaa aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac
    23161 gattagataa aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt
    23221 cgatctcatc tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct
    23281 tggtcccatt aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca
    23341 gataattctg ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc
    23401 tgtatgtact aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt
    23461 ccacaaataa aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg
    23521 cttttcaatt tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt
    23581 acttaagctt aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt
    23641 attttggtgt tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt
    23701 tgttttcaat cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct
    23761 gtttttttct ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat
    23821 atcgtttgcc tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca
    23881 gtataggcaa tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc
    23941 attcaggagg aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata
    24001 ctcattttga caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa
    24061 gtaaaaacat cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa
    24121 aaagaagtgt tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa
    24181 gtcaaagaag ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat
    24241 caactctatt ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc
    24301 aaattgaatg atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa
    24361 actgttaaag ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat
    24421 aacatggaaa attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat
    24481 attctagttg ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta
    24541 aacgaaagca tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat
    24601 aaacaagcca ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac
    24661 tttttcagta caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg
    24721 gaagcttttg aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata
    24781 gcaatactgt tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa
    24841 atcaaagaat tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac
    24901 agtgaaatac tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct
    24961 ttagcaggaa caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga
    25021 ttgctgatcg gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt
    25081 ctgatcatcg gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa
    25141 cgtcctgaga tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt
    25201 ttagttgcag caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc
    25261 aatgatattg aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt
    25321 gaaacagata aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa
    25381 gcaaaaatga gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt
    25441 atgttacaat gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa
    25501 aagaaaagtg cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat
    25561 ataccagtag gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta
    25621 tcaggactgt tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg
    25681 cttacgacgc aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc
    25741 tatacattat taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg
    25801 gctgtctttt ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca
    25861 actgaccctt cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca
    25921 tgggccggta gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac
    25981 cttgtccatt ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc
    26041 gcactactga tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta
    26101 ttccaaaata ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg
    26161 ctggagaagg cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc
    26221 aatgagccgc tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt
    26281 gctactttta gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct
    26341 ttattttact catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt
    26401 ggcattattt gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat
    26461 tgcttggaat atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg
    26521 ttcgatggcg ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta
    26581 aactgtttta ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc
    26641 gaactgcatt tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt
    26701 agtgttggct tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt
    26761 ctatgatttt ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat
    26821 ttaaatgaat aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg
    26881 taaagtttta ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga
    26941 tggtatgctt gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat
    27001 gcttggtaca gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga
    27061 aggagataac gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat
    27121 cggtatcgcc tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc
    27181 gattgctaac gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc
    27241 tatcccaatt cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta
    27301 tggaacttct ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta
    27361 ttacttgcgc gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc
    27421 ttctttcttc ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga
    27481 cttcttcgaa cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc
    27541 tgcctctgtc tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac
    27601 tatcgttcaa atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga
    27661 gatcgcagat cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt
    27721 gacattgatc ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag
    27781 cacgatgaag aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat
    27841 tgcgttattg attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata
    27901 cacattgttt tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc
    27961 ttctttgttt ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca
    28021 tcgttttgcg atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat
    28081 cggattagga ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat
    28141 tgtctttgtt cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa
    28201 gatcagcaat tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt
    28261 tgccgttttc tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat
    28321 ttattgtgtg gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa
    28381 aaatgggttt cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa
    28441 ctaatcgttt tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt
    28501 tctattttcc aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg
    28561 aaaaattctt tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc
    28621 ggcatatctg ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt
    28681 gattatggat gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg
    28741 acttttaaaa aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt
    28801 gggtttatat gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc
    28861 ttcagggatt tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt
    28921 aacggtcatt ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc
    28981 tgccttagac ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg
    29041 aatccgagat aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg
    29101 aggacgtttg acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct
    29161 gacattttct gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt
    29221 tgctcgagct gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact
    29281 ttacgtgata catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa
    29341 aaaatatcca aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt
    29401 aggtgaaaaa gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca
    29461 taccaaaaat attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa
    29521 gaacgagatg gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc
    29581 aatcctctat aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta
    29641 ctaaaaataa aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa
    29701 ccagtaatcg ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggat
    29761 gcaaaagggc tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca
    29821 ggcaaggtat ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc
    29881 acacgtattt tagatttagc gatcttagct gcacaaggaa gtttatactt ccaagatgct
    29941 tatcgttatg aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa
    30001 gggaaccgaa tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt
    30061 ttgtattacg atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta
    30121 aaacgtcttt tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa
    30181 gcatggagaa tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga
    30241 tctggaagaa accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa
    30301 tcaaagggaa ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg
    30361 ccgggtcggg gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata
    30421 caaaagtgac ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg
    30481 tagtagattc taataaggaa caagaccaag aattactaga agctatttac caaacagata
    30541 cacttgaagc tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta
    30601 aagaaaaatt agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt
    30661 acagctatta cccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc
    30721 aagagccgac tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac
    30781 caaatgggat gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg
    30841 tgatcactac aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc
    30901 ggatgcaaca tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga
    30961 tggcgaaaac tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt
    31021 tgttggatga aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt
    31081 tttattgggc aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag
    31141 ttttgtataa attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg
    31201 acagtggatt gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc
    31261 aatctgagca tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca
    31321 ctgaaaatgc tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac
    31381 caaatgaggg agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa
    31441 tgcaaaaaga acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag
    31501 atgattctca agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc
    31561 aactgaataa agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca
    31621 tcgttcaccg accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag
    31681 taatgccaat agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa
    31741 cagagacgta ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat
    31801 atggtgattt gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg
    31861 tagctgtacg gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa
    31921 aaaaagcagc gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt
    31981 acggagatac agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat
    32041 ttgaagacgg atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca
    32101 atcgtgatgg tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa
    32161 ccaaactgtt gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca
    32221 aaaaagcaaa agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat
    32281 ttgtctccgc agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc
    32341 gttttgtaga tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc
    32401 gctatctatg ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt
    32461 ttctagttac cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc
    32521 gttaaaggag tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta
    32581 tcaaatctat cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg
    32641 gatccgtgaa aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc
    32701 gatctatcaa tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga
    32761 agagagattc ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg
    32821 gatcaaagtg attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga
    32881 agaatcgaaa aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt
    32941 agatggcaaa gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc
    33001 tcatgaaact ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg
    33061 ggaaagcgag aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg
    33121 gatagatgga tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa
    33181 agccacagag aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac
    33241 cgatttgaaa catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt
    33301 cactgcagaa gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga
    33361 atttgatcat atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt
    33421 gaaacttgca ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata
    33481 tatggaaaac catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt
    33541 ttgggctatg tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt
    33601 tatttatcaa ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt
    33661 tgatgcagta gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga
    33721 agaagcattg gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg
    33781 gacttcggaa caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa
    33841 taccaaaacg atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta
    33901 taagaacatg atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga
    33961 atatcttcat gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata
    34021 tcttattatg gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga
    34081 tcagtcctgg acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat
    34141 actagcacct tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt
    34201 tcattagttg gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat
    34261 aattcgctct aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt
    34321 gtccaagggt tttcaaacga ggtctgacag gtacttggac atgtttgata tgcataccga
    34381 ttgatgtgtc accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat
    34441 cattgaactg gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa
    34501 cagagacaat ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt
    34561 tcaaatgttc acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta
    34621 acgtacggat gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa
    34681 cgacttcgct tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag
    34741 ccaatatgtc attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca
    34801 ttttctaaaa ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt
    34861 gcagttatag gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata
    34921 gaagccactc cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc
    34981 ataactaagc ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa
    35041 gaaccagcaa catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga
    35101 tatcctgaaa tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt
    35161 ggaccataaa gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc
    35221 gtagccggta cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata
    35281 gcatagatcg ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga
    35341 ccagacactt ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa
    35401 ttttttactt tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat
    35461 tgcagaagaa aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga
    35521 aaatacctca taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct
    35581 tgtgccgcct ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg
    35641 gatcatttct gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa
    35701 attaggcgta atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa
    35761 ttgattgatc acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat
    35821 attttgtttt gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa
    35881 cccaattttg ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag
    35941 caattccaaa gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga
    36001 agtcaaggcg cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat
    36061 tcctccacca gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc
    36121 ctccatgaat aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt
    36181 ttatctcatc caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc
    36241 cttaaatagt taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt
    36301 cattgtagct gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg
    36361 aagttcatgg acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc
    36421 aataatcggt tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc
    36481 taacgaatct aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc
    36541 cacttgccga tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct
    36601 atcccaccaa tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa
    36661 agcccgtcct ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc
    36721 aactgaatct gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc
    36781 atcacttgtt ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact
    36841 ttggccatat acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt
    36901 cattgtctgg ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc
    36961 ttctggatgc tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag
    37021 tgcaattggc cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa
    37081 aggcatcata ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg
    37141 cactggagag atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt
    37201 ttgccatttc gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc
    37261 agttgtacca gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga
    37321 aacagacaaa gcaggttgtt ccattaaata aagaatagga atacccgtgg tgctagttaa
    37381 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
    37441 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
    37501 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtctc aactgttttt
    37561 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
//
LOCUS       DAYYGS010000018        36452 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000018 DAYYGS010000000
VERSION     DAYYGS010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.018"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..3654
                     /locus_tag="ACHA44_000862"
     CDS             127..3654
                     /locus_tag="ACHA44_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8904173.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="ACHA44_000863"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="ACHA44_000863"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HFM8904174.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="ACHA44_000864"
     CDS             7442..7783
                     /locus_tag="ACHA44_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFM8904175.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA44_000865"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="ACHA44_000865"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFM8904176.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA44_000866"
     CDS             9140..11560
                     /gene="pheT"
                     /locus_tag="ACHA44_000866"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFM8904177.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA44_000867"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="ACHA44_000867"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFM8904178.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="ACHA44_000868"
     CDS             12994..13731
                     /locus_tag="ACHA44_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8904179.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="ACHA44_000869"
     CDS             13745..14560
                     /locus_tag="ACHA44_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8904180.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="ACHA44_000870"
     CDS             14573..15220
                     /locus_tag="ACHA44_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8904181.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="ACHA44_000871"
     CDS             15236..15892
                     /locus_tag="ACHA44_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8904182.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="ACHA44_000872"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="ACHA44_000872"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFM8904183.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="ACHA44_000873"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="ACHA44_000873"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFM8904184.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="ACHA44_000874"
     CDS             18234..18752
                     /locus_tag="ACHA44_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8904185.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA44_000875"
     CDS             18859..19359
                     /gene="cbpB"
                     /locus_tag="ACHA44_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HFM8904186.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="ACHA44_000876"
     CDS             complement(19639..20559)
                     /locus_tag="ACHA44_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFM8904187.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20685..21737
                     /locus_tag="ACHA44_000877"
     CDS             20685..21737
                     /locus_tag="ACHA44_000877"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HFM8904188.1"
                     /translation="MIKCGENNEGGKYMQIDWNNLGFSYIKTPWRFIAKWKDGQWEEG
                     ELTEDNYLTIHEGSPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLM
                     PMVPEKMFVDAVKQVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVF
                     CTPVGAYFKGGLKPTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCV
                     YLDPATHTKIEEVGAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIE
                     GDIFIDDLDQFAEAGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGI
                     QFGDIEAPEGWIEVVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHA44_000878"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="ACHA44_000878"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFM8904189.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHA44_000879"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="ACHA44_000879"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFM8904190.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="ACHA44_000880"
     CDS             complement(23377..24138)
                     /locus_tag="ACHA44_000880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8904191.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="ACHA44_000881"
     CDS             24327..25421
                     /locus_tag="ACHA44_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HFM8904192.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25507..26466)
                     /locus_tag="ACHA44_000882"
     CDS             complement(25507..26466)
                     /locus_tag="ACHA44_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HFM8904193.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            26652..27587
                     /locus_tag="ACHA44_000883"
     CDS             26652..27587
                     /locus_tag="ACHA44_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8904194.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            complement(27672..28682)
                     /gene="gap"
                     /locus_tag="ACHA44_000884"
     CDS             complement(27672..28682)
                     /gene="gap"
                     /locus_tag="ACHA44_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8904195.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            28970..30283
                     /gene="obgE"
                     /locus_tag="ACHA44_000885"
     CDS             28970..30283
                     /gene="obgE"
                     /locus_tag="ACHA44_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFM8904196.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            30504..31091
                     /locus_tag="ACHA44_000886"
     CDS             30504..31091
                     /locus_tag="ACHA44_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HFM8904197.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            31268..32209
                     /gene="rnz"
                     /locus_tag="ACHA44_000887"
     CDS             31268..32209
                     /gene="rnz"
                     /locus_tag="ACHA44_000887"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HFM8904198.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            32206..32994
                     /locus_tag="ACHA44_000888"
     CDS             32206..32994
                     /locus_tag="ACHA44_000888"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8904199.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            32995..33438
                     /locus_tag="ACHA44_000889"
     CDS             32995..33438
                     /locus_tag="ACHA44_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HFM8904200.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            33580..35877
                     /gene="recJ"
                     /locus_tag="ACHA44_000890"
     CDS             33580..35877
                     /gene="recJ"
                     /locus_tag="ACHA44_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFM8904201.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            35891..36403
                     /locus_tag="ACHA44_000891"
     CDS             35891..36403
                     /locus_tag="ACHA44_000891"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFM8904202.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
BASE COUNT        11945 a         6318 c         7840 g        10349 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tataggttat
    25501 gatttgtcaa attaagctag aataaaatgc ttcttgtaca tagctcaaaa atatttcgat
    25561 tggtgttctg taattcaatg attttcttgg aatatgatta cgttgattgc tgacactgga
    25621 aataaatgtc tgattcactt ttctaaaatc cattgatttc ggcagtccat tacgacgcag
    25681 aatcccgtta gaattctcgt ttaatgggcg ttgagaaggt gttccagggt ccgcaaaata
    25741 tatatcaata tcatgttggt tactaatggc tttccagtta gaaaattctt ttccacagtc
    25801 aaacgtaata gatttaaaga agtttttagg gaagcgagaa aaccattgat taagggcagt
    25861 ttcaatatct aatgccttac ggccgttggg tttaatcgtg ataatgactt tagataatct
    25921 ttcaactaaa gtaatgacgg cacttttatg atgaatgcca acgatcgtat caccttcaag
    25981 gtgaccaaac tcagaattga aatcaggata attatcaggt cgatcatgga ttgagcgctg
    26041 atactgttgt tttccccgtt tttcctgatg gccattgggt tttcttttac ctttcatcgg
    26101 tagtgtgtca atatcaaata ttcctttaga aaataaacga taaagtgttc tcataccgca
    26161 tgaaacagac ctttcttttc gcccgataat gacgtcaggc gtccaaccaa gagtgacttt
    26221 ctctttaatg taatctactt catgagcagg taattgaatg acttttctac cacatttttt
    26281 cttattttct ttatactggt gccaataatc aagagcagtc ttgccttgct tgaacttatt
    26341 gactacatta taaatagttt gtatagcacg tcccattcgg ttagcgattt caaccggctt
    26401 attatgttga agataatatg attctatcat tgtcagttcg tctatggtaa gatgtttgta
    26461 ggtcatttat ggttacactc ctttgttttc tttcgtcgga aatacaattt gagtgtacca
    26521 taaatgcctt tttatttttc taacttaatt ttacaattcg cgggttataa aaatgaacat
    26581 tgcatagtag agattctttg attatactaa ttcaaggtta tatgaaaaaa gagaaatgag
    26641 atggatgatt tatgaaactt tggatgaaaa tccttttagg cgtcgcttta gctctgattg
    26701 ttgcgttagt gtttgcaggg aattattttt ataattatgc tgttgtgcct tctgagaaag
    26761 actttttaga cgaagataca cctggtacaa cacagacatc caaagaaacg gatgcacata
    26821 aatggtttag ttcaaaagaa aaccgaagcg attggaccat cacttcaaaa gacggtctga
    26881 aattatcagc gatttatctt cctgctgaaa cgaaaagtga aaaaacagct cttgtggctc
    26941 atggctatat gggaaacgct gaaacgatga ccaattacgc aaagatgttt catgatatgg
    27001 gttacaatgt ccttgttcct gatgcaagag gacatggaaa aagcgaaggg gattacattg
    27061 gatttggatg gccagagcgt aaagactatg tacagtggat aaacaaagtg ctagaaaaca
    27121 atggaaaatc gcaagaaatc gttctatacg gtgtcagcat gggagcagca acagttatga
    27181 tgactagcgg agaaaaacta ccagataatg tgaaagcaat cattgaagat tgcggttatt
    27241 cttccgttca tgatgagctt gcttatcagc tagacgatat gtttagtctt ccagcatttc
    27301 cgcttatgca agtaacgagt ctagtgacaa aagtccgtgc tggctacttt ttcggtgaag
    27361 caaatgcagt ggaacaattg aagaaaaacc agcgcccaat gctctttata catggagacg
    27421 aagatacatt tgtaccattt gaaatgttag ataaggtcta tcgtgcaacg aagggaccaa
    27481 aagaaaagta tgttgtaaaa ggagccgagc atgcggaagc atacaagaca gatcctgaaa
    27541 aatatcagca agtagtccaa caatttttaa gccaatatgt tcattgaacg agtttaaaag
    27601 taggggatga aaagaaagaa gcctttgact ggatccagtc aaaggcttct tttatcgatt
    27661 gatctatcaa attacaagtt agcgaatttt tctagtaagc gaatcatttg gcaagtaaat
    27721 ccatactcat tgtcatacca tgcaacagtt ttaacaagtt ggaaatcacc agcagttgtt
    27781 acttctgttt gcgttgggtc aaagatcgaa ccttgagttg ttccaatgac atcgctagaa
    27841 acgatttgac ggtcatcata cccgaatgaa ggattgtcaa ctgtgtgttt tttgattgct
    27901 tcgtttactt gatcagcagt tacttttgtt ttcaatactg aaaccaattc agttaatgaa
    27961 ccatcaacga ctggaacacg ttgtgcgtgt ccttgtaatt ttccttgtag ctcagggata
    28021 actaagccga ttgctttcgc agcaccagta gaatgtggga tagtattgtc agcagctgaa
    28081 cgagcagcac gcaaattacc gccttttaca ggtccatcta agagcatttg tgtggaagtg
    28141 taggcatgaa ctgttgtcat tgtaccaact tcgatcccaa attcattatt taagaaataa
    28201 gccataggtg ctaaacagtt tgttgtacat gaacctgctg agatgatttt gtcgttttca
    28261 tctaatgtat catcattgac gttatagacg atggttttca tagcaccagc tggggcagaa
    28321 ataactactc gtttgacacc agcatctaaa tgcgcttttg ctttttcttc agatgtataa
    28381 aaaccagtac attctagtac gatatctaca ccgttttctt ttgcccaagg gattttgctt
    28441 gcgtcgggtt ctgcataaac acgagttgct tcgccatcca ctacgattga gtcttctgtt
    28501 gcagtaactg tgccaggata agtaccatga gtagagtcaa attgcaataa ttgtgctaac
    28561 atagttggac ttgttaagtc gttgattgct actacttcaa tatcatctga gacttctttg
    28621 atgcggcgga aagccagacg gccaatacgc ccaaatccgt taatacctac ttttactgtc
    28681 atatcaaaag ttcctccttt attaggggtg ctgtgaaacg gcatcctcaa ttaatacaat
    28741 taatacactg ttattctgct acgcaaagaa tattttgtca aaaaattata cttggtagca
    28801 gaaaaaaggt tggaaacctt accaaatgat aaaaaaagaa gaaggtaaac aatctttttg
    28861 ctgaaatgat agacaaaatc agatttcacc gataaaataa gtaagacttt tgtttattgt
    28921 ggaaattcag gcagtcaaat aaataaatga ataataggag gacttaatta tgtccatgtt
    28981 tttagatcaa gtaacgatcg atgtgaaagc tggcaagggc ggagacggaa tggttgcatt
    29041 tcgtcgcgaa aaatacgttc ctgacggcgg accggctggc ggagacggtg gtcgtggcgg
    29101 cgatgtcatc ttgatcgtcg atgaagggtt acgtacattg atggattttc ggttcaatcg
    29161 ccatttcaaa gcgcagccag gtgaaaatgg gatgagtaaa gggatgcacg gtcgtggttc
    29221 agaagatacc tatgttaaag taccacaagg aaccactgtc cgagatgcgg agactggtgc
    29281 tcttttagga gatttaattg aaaatggtca aacactagtt gtggcaaaag gtggacgcgg
    29341 tggacgaggg aatatccgtt ttgcctctcc tcggaatcct gcaccggaaa ttgctgaaaa
    29401 cggtgaacca ggacaagagc gaaaaatcga actagaattg aaagtattgg cagacgttgg
    29461 acttgtaggt tttccatctg tcgggaaatc gactttacta tccgttattt catctgctcg
    29521 tccaaaaatc ggcgcttatc atttcacgac gctcgtacct aatctaggaa tggttactac
    29581 aagtgatggc agaagttttg ccgcagcaga cttgccaggc ttgattgaag gagcttcgca
    29641 aggtgttggg ctgggtacac aattccttcg tcatatcgaa agaacacgag taattttaca
    29701 tgtgatcgat atgagtggga tggaaggacg agatccttat gaagactatc ttgccatcaa
    29761 caaagaatta tctacttata acttacgctt gctagagcgt ccgcaaatca tcgtggcaaa
    29821 caaaatggat atgccggatg caccggaaaa tctagtgaaa ttcaaagaac agctgaacaa
    29881 ggaaaaagaa gatgaatttg ctgatgacat cccagtattt ccaatttctg gagtgactcg
    29941 acaaggattg gacgcgttat tgaatgctac agcagatttg ttagaagtaa cgccagaatt
    30001 cccattgtac gaagaagaac tggaagaaga aactgtacac tatggattca atccagaagg
    30061 tcctgagttc caaatcgatc gtgattcaga tgcaacgtgg atcttatctg gtgagaagat
    30121 cgaaaaatta ttccaaatga cgaatttcga tcatgatgaa acagtgatgc gttttgctcg
    30181 tcaattacgt ggtatgggtg tggatgaagc tcttcgggca cgcggtgcga aagatggcga
    30241 ccttgtacgt atcggtgagt ttgaatttga atttgtcgaa taggcaagca aataaaaaca
    30301 aataccaaaa aatatagatt cagatttaag ggaggttgtg acagaagtga tcagcttcaa
    30361 gaacgccgtt tattcggaaa aaggagctgt gatacgactt ttgtcacagc cctattttat
    30421 tggaaaaaaa gagtgctttt cttttcttat ttttcaatta aaggtaaaat aaataacaag
    30481 aaggtatgaa aggggcgttt ttaatgagca aacgagtaat cattttaaat tttcaagtag
    30541 aaagccaggc atatcaggca ttttcagaaa tcaaaaagtt acatgctgtt cgttcgatta
    30601 aaggagaaca gatggcagtt gtcagccatg atcctaatgg gacacatcaa ttcaaaatag
    30661 aagattttat cgactttacc ggtaataaca aatcttctac gggtggatta atcggaatgt
    30721 tggttggtgt attaggtggt ccgctaggga ttttattagg atggtttgcc ggaggaatga
    30781 tcggtgcgac acaagatgcc aaagaaataa aagatgccca gaccgtgttc gactttgtag
    30841 gaaataaaat cggtgaagga gctacaggac tgatcttgat tgctgatgaa gaggacaatc
    30901 gcccactgaa tcaattggta atgatggaat taggcggaga aattactcgc ttggattatg
    30961 aagatgtcga aaaagatgtg catgatgcga aagaagctga aaatcatgcg aaagaagctg
    31021 cacaaaagac ttggaaggac aaacatccgc aaacttccaa cgacaatgaa agttctggat
    31081 cttcaaatta ataaaaaaca gcagaggatc tcaaacgggt tcgctgctgt ttttatagta
    31141 attttcaaag aaaacgctat cgctttagaa cgttattttt aatttgaacc cattctcgac
    31201 ttacggttga ttatcccttc tttttaccct aaaattaaag aggaacttaa agaaagtcag
    31261 gtagattatg gaattacaat ttttaggaac aggtgcaggt gttcctgcaa aacatagaaa
    31321 tgtgacaagt atagcattga aacttttaga cgagcgcaat gaagtttggt tgttcgactg
    31381 tggcgaaggc acgcaaatgc agattttgcg aacgactatt cgtccgagaa aaatcggtaa
    31441 aattttcatt acacatttac acggcgatca tatttttgga cttcccgggc tcatcagcag
    31501 ccggtcattt caaggtgggg atacgccatt agagatttac gggccaaaag ggatagagga
    31561 atacataaaa gtctctttag gtatttcgca aacgcgttta agttatccgc tgaaattcat
    31621 tgagctaaat gaaacagccc cgatttttac tgatcagcaa ttttctgttt atacaaaaaa
    31681 actaaatcat gggatcgaca gttttggtta tcgtgtggtt gaacatgatc ataaaggaga
    31741 attgcaggtg gatcgtctaa aagaattagg tattccagct ggaccactgt acggcaaact
    31801 gaagcaaggt gaaacgatcc aactagaaga tgggcggacg atcaatggaa aagatttcgt
    31861 aggtccagat aaaaaaggac gaatcgtgac tattttaggc gatacgagaa aaacacataa
    31921 ttccgttgtg cttgcggaaa atagcgatat tctggtacat gaaagcacat ttaataaaga
    31981 tgaagcaaga atggcgcaca actatttcca ttccacgact catcaagctg ctgaagttgc
    32041 aaaagaagcg caggcaaaac ggctgctact tactcatatc agtgcgagat atttaggaaa
    32101 agcagcttta gagcttgaaa aagaagcaca agaagtgttt gaaaacacga cgattatgaa
    32161 agattttgat agcattgaga ttccatttcg cgaggaggaa gaggcatgaa tttagaagat
    32221 aaagttgttt tagtaacagg tagttcaggc ggtctgggcg cacaaatctg ttatgaagca
    32281 gcaaagcaag gagcaatcgt tataagttgc gcacgaagaa tggcatttgt agaaggtgtc
    32341 agagacgagt gcagacgctt gagcggcaaa gaggcttatg ctttcaaggt agacgtcagc
    32401 aatcctgaaa gcgtggacga attatatgaa aaggtgatgg aagaagtcgg gcgcgtcgat
    32461 attctggtaa acaatgcggg ttttgggatt ttcgaagact tccttacatt cgaccttgga
    32521 aaagcatacg atatgtttga agtcaacatc ttaggaatga tggttttgac acaaaaattt
    32581 gcgatcgata tggcagaaag aagacaaggt catatcatta atatcgcctc tatggctgga
    32641 aaaatggcaa ctgcaaaatc aacggtttat tctgccacga aatttgcagt ccttggcttc
    32701 tccaacgctt tgcgattaga attgaaacca cttggcgtag ctgttacgac agtgaatcca
    32761 ggtccaatcg agacaaattt cttcgataag gctgatccaa gcggaagcta tttggaaaaa
    32821 gtcggacaaa tcgttttaga acctgccaag ttggcaaaaa tgatcgtccg caatatgaga
    32881 catccaaaac gggaaatcaa tcaaccgttt attttagaag ttgcatcaaa attttatacg
    32941 ctgtttccaa cgattggtga ttatttagcc agcggaattt ttaacaaaaa gtaggtgaag
    33001 gaattgaaaa agcaaagcaa tgtgattctt ggatttgttt tagttttgat tattgttctt
    33061 tttgctgtat tgaacaataa aaatgttcct gtaagttttg gcttcacttc cttttctgcg
    33121 ccattgatct tagtgatcat cggttctgcg ctgattggtg cactgattgt atttttgact
    33181 gcttcggcaa cactatggca gcaaaaaaag caaatcaaac agctgactca ggaaatgtca
    33241 gaatatcaaa cagaacttca aaagaaaatt gaagaagcaa aagaagaaca gcaacgcgag
    33301 tttagaaacg aacgtgcgga gttggaagct gcttatcagg cggagttaca agcgaaaacc
    33361 gcacaaatca gtcaattgaa agcgccggaa gaacaaaaaa ctcaatcgcc caaacaaagc
    33421 ttcaattatt ttgactaata gggatatttc ataaatggtc gtacaaaaga tcaggtcctg
    33481 ctgaacatct gtctagcgga tctgatctgt ttttattctt tctttctgct tttttttgct
    33541 gatttccttt gctataataa ctaagttagg agtgagaaag tgagacaagc aaattttgat
    33601 tggaagttaa gcgagacaga gccttctgaa tcctttctcc aaatgatccg tgaactgaaa
    33661 ttatctccat ttgtcggtaa gttactgtgg caaagaggtt accatgagga aaaggatatc
    33721 aaccgatttt tacatccaaa ggaacaagaa ttacatgatc cgtatctgat acacgatatg
    33781 gataaagcaa tcagccgaat ccaagaagct gtaatcagtg gagaaaaaat cctcgtttac
    33841 ggtgattatg acgcagatgg gatcaccagt gcaacggtaa tgaaagaaac actggaatta
    33901 cttggtgcag aagtagaagt attcctgcca aatcgatttg agcatgggta tggaccaaac
    33961 caatcggtat accaagaaaa gatcgaagaa ggaatccagt tgatcatcac tgttgataac
    34021 ggggtagcag gaaatgatgc ggtagcatac gctcagcaag ctggtgtaga cgtgatcatt
    34081 acagatcatc acgaactacc tgagaattta ccagacgctt atgcgattgt tcaccctcgt
    34141 catccagcgg ggcactatcc atttccagat ttggcaggag tcggagtagc attcaaagta
    34201 gcttctgcat tattggaaga accgcctgca gaatttttgg atttagtagc gataggaacg
    34261 atcgccgatt tagtttcatt gactgatgag aatcgaatcc ttgtttcatt aggaatcgat
    34321 gccattcatc attcagaacg gataggactt caggcgctat ttgaagaaag cggcgtaaaa
    34381 atgagggacg cagacgaaac aacgatcggt ttttcgattg ctcccagact aaatgcaatc
    34441 ggtcggatgg gcgatccacg tcctgccgtc tcgttacttg cgacgtttga tgaagaggaa
    34501 gcatccgttc aagcaaaaaa attgaatgag atcaatgaag aaagaaaagc aatcgtagag
    34561 cagataacac aagaagcatt agcgatggtc aataaggaga atcatatcca tctattggta
    34621 aaccgaggat ggcatgaagg cgttttgggc attgttgctg ggaaaataat gaatgaaacg
    34681 ggtaaaccaa cgcttgtcct tactttaaaa gaagatggat cagctaaagg atctggacgt
    34741 agtattgaag ccttgaatct ttttgaaatg cttgaccaga tgcgagattt atttacttac
    34801 ttcggaggac atcatgcagc agtcggttta acgatgccaa gtgaaaatgt cactatacta
    34861 caggagaaga tgaaccaata tatagtcgat catcaaattg acttgatgag agggcctgag
    34921 ttgagaattg atgaagtatt gctgccaaat gaagtaacag tagagcgaat tgatgagctg
    34981 aaactattgg cgccatttgg tacggataat cctttgcctc agtttttatt tcgacaagtc
    35041 caagcagaaa atatcaaaag aatcggagca aaccaacagc atctgaaatt tgtcctttcc
    35101 gatgcatcga gtcagcttga tgcggtcgct tttggttttg gtactcaaga agaagaactg
    35161 ctgaataatc aggtagatgt ggtaggaaag ctttcgatca atgaatggaa cgggcgtaag
    35221 aaaccgcagc tgatggtatc tgatttttct gtgggtggtc tgcaagtatt tgattggcgg
    35281 gcaaaacgtt tccgtgaaca gagccgaacg tcggaaggta gcctttattt agcatttgat
    35341 tcttcgacgt tgaaattcgt gcctgagatt atccaggaag ctgcgattgt tttcgaagga
    35401 ctagattctg taaaggcaat aattgaaaaa agaaaaccaa actctctagt tgttatggat
    35461 tgtccagatg atttagcgga tctgaaagaa gtttttcggt cttattcatt caatcgtgtt
    35521 tatctgatgg ggatttcacc tgatgaggcc tatttgaatg gggtaggcac aagagaacag
    35581 tacgcaaaat tgtttaaatt gatccattct caagaacgaa ttgatatacg tcataaaata
    35641 aaagcaatag ctcaatattt gcgtatccct gaaaaattat taatttttat gatacaggtg
    35701 ttttttgaat tgaaatttgt tacaatagag aacggtgttt tacaaaaagt cgctagccca
    35761 gaaagtcatc ctttgactga gagcagatta taccagcgac ggctcaataa aatcaaagtt
    35821 gaggaatttt tgcttttgag cgatattcct accataaaga aatggttaac aacctaggag
    35881 gaaacaaaga gtggatttaa aagattatat tgccagtatt cccgactatc cgtcaaaagg
    35941 aattgtgttt cgagatattt ccccactgat ggcagatggt gacgcttatc gggaagctac
    36001 taaacaaatc gtaaattatg caaaagaaaa acgaattgat atggtagttg ggccagaagc
    36061 ccgtggattc atcgtaggat gtcctgttgc ttatgagtta ggagtcggtt ttgccccagt
    36121 ccgcaaaaaa gggaagttac ctcgagaaac catcgaagtg acttatggat tagaatatgg
    36181 aacagataca ttgacattgc ataaggatgc cattaaacca ggtcaacgtg tgctgatttg
    36241 tgacgatttg ctagctactg gaggaacaat caaagcgacg atcgaactga tcgaacaatt
    36301 aggcggtgta gtcgtaggct gcgcattttt gatcgaattg atggatctcc atggacgcga
    36361 caaaatcaaa ggatatgaca tcattacatt aatggaatat taagcagaaa aaagaagcta
    36421 ttcttgatgt tattcttaaa gaagcgattt ta
//
LOCUS       DAYYGS010000019        35114 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000019 DAYYGS010000000
VERSION     DAYYGS010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35114
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.019"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(64..837)
                     /locus_tag="ACHA44_000892"
     CDS             complement(64..837)
                     /locus_tag="ACHA44_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904203.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            942..1187
                     /locus_tag="ACHA44_000893"
     CDS             942..1187
                     /locus_tag="ACHA44_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8904204.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            1343..2194
                     /locus_tag="ACHA44_000894"
     CDS             1343..2194
                     /locus_tag="ACHA44_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HFM8904205.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            2227..2904
                     /locus_tag="ACHA44_000895"
     CDS             2227..2904
                     /locus_tag="ACHA44_000895"
                     /inference="COORDINATES: protein motif:HMM:NF024685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HFM8904206.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPHY
                     CIMRKIL"
     gene            3392..4357
                     /locus_tag="ACHA44_000896"
     CDS             3392..4357
                     /locus_tag="ACHA44_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6017 domain-containing protein"
                     /protein_id="HFM8904207.1"
                     /translation="MAVFRVEKNKGYTVMSNHHLRNKNLTLKAKGLLSQMLSLPEDWD
                     YTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDAKGKMADMVYTIYEQPVLENP
                     VLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLSKKEKIITDGQSTHSIPIPSP
                     TPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEYAYLIQDRSIDREQLDEIVDL
                     MLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRFVLDCLRENTTKVRNIKQYLR
                     AMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYSCGEGESL"
     gene            4379..4591
                     /locus_tag="ACHA44_000897"
     CDS             4379..4591
                     /locus_tag="ACHA44_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8904208.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            4666..5142
                     /locus_tag="ACHA44_000898"
     CDS             4666..5142
                     /locus_tag="ACHA44_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HFM8904209.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            5139..6947
                     /locus_tag="ACHA44_000899"
     CDS             5139..6947
                     /locus_tag="ACHA44_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirD4-like conjugal transfer protein, CD1115
                     family"
                     /protein_id="HFM8904210.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            7024..7239
                     /locus_tag="ACHA44_000900"
     CDS             7024..7239
                     /locus_tag="ACHA44_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family mobile element protein"
                     /protein_id="HFM8904211.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            7331..8194
                     /locus_tag="ACHA44_000901"
     CDS             7331..8194
                     /locus_tag="ACHA44_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB6/TrbL-like conjugal transfer protein,
                     CD1112 family"
                     /protein_id="HFM8904212.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            8231..8767
                     /locus_tag="ACHA44_000902"
     CDS             8231..8767
                     /locus_tag="ACHA44_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002604480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFM8904213.1"
                     /translation="MSTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVERL
                     ASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTRGN
                     AEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFARQK
                     TPGWDVWGNEVECDLILT"
     gene            8781..9203
                     /locus_tag="ACHA44_000903"
     CDS             8781..9203
                     /locus_tag="ACHA44_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HFM8904214.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            9133..11532
                     /locus_tag="ACHA44_000904"
     CDS             9133..11532
                     /locus_tag="ACHA44_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HFM8904215.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            11564..13555
                     /locus_tag="ACHA44_000905"
     CDS             11564..13555
                     /locus_tag="ACHA44_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFM8904216.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            13578..13829
                     /locus_tag="ACHA44_000906"
     CDS             13578..13829
                     /locus_tag="ACHA44_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HFM8904217.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            13819..15048
                     /locus_tag="ACHA44_000907"
     CDS             13819..15048
                     /locus_tag="ACHA44_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1107 family mobile element protein"
                     /protein_id="HFM8904218.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            15045..17126
                     /locus_tag="ACHA44_000908"
     CDS             15045..17126
                     /locus_tag="ACHA44_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HFM8904219.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            17275..21195
                     /locus_tag="ACHA44_000909"
     CDS             17275..21195
                     /locus_tag="ACHA44_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YodL domain-containing protein"
                     /protein_id="HFM8904220.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKTD
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNDYYRKHGTLDGCPHLIERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            21196..22140
                     /locus_tag="ACHA44_000910"
     CDS             21196..22140
                     /locus_tag="ACHA44_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904221.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            22144..22347
                     /locus_tag="ACHA44_000911"
     CDS             22144..22347
                     /locus_tag="ACHA44_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposon-transfer assisting family protein"
                     /protein_id="HFM8904222.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(22647..22883)
                     /locus_tag="ACHA44_000912"
     CDS             complement(22647..22883)
                     /locus_tag="ACHA44_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904223.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(23066..23437)
                     /locus_tag="ACHA44_000913"
     CDS             complement(23066..23437)
                     /locus_tag="ACHA44_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HFM8904224.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(23434..24762)
                     /locus_tag="ACHA44_000914"
     CDS             complement(23434..24762)
                     /locus_tag="ACHA44_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8904225.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(24723..25052)
                     /gene="mobC"
                     /locus_tag="ACHA44_000915"
     CDS             complement(24723..25052)
                     /gene="mobC"
                     /locus_tag="ACHA44_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8904226.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(25310..25680)
                     /locus_tag="ACHA44_000916"
                     /pseudo
     CDS             complement(25310..25680)
                     /locus_tag="ACHA44_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            25825..26010
                     /locus_tag="ACHA44_000917"
     CDS             25825..26010
                     /locus_tag="ACHA44_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8904227.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            26168..26335
                     /locus_tag="ACHA44_000918"
     CDS             26168..26335
                     /locus_tag="ACHA44_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904228.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            26953..27612
                     /gene="vanR-B"
                     /locus_tag="ACHA44_000919"
     CDS             26953..27612
                     /gene="vanR-B"
                     /locus_tag="ACHA44_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8904229.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            27615..28976
                     /gene="vanS-B"
                     /locus_tag="ACHA44_000920"
     CDS             27615..28976
                     /gene="vanS-B"
                     /locus_tag="ACHA44_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8904230.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(29067..30368)
                     /locus_tag="ACHA44_000921"
     CDS             complement(29067..30368)
                     /locus_tag="ACHA44_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8904231.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            30647..31453
                     /locus_tag="ACHA44_000922"
     CDS             30647..31453
                     /locus_tag="ACHA44_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8904232.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            31471..32178
                     /locus_tag="ACHA44_000923"
     CDS             31471..32178
                     /locus_tag="ACHA44_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8904233.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            32175..33146
                     /locus_tag="ACHA44_000924"
     CDS             32175..33146
                     /locus_tag="ACHA44_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8904234.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            33139..34167
                     /gene="vanB"
                     /locus_tag="ACHA44_000925"
     CDS             33139..34167
                     /gene="vanB"
                     /locus_tag="ACHA44_000925"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8904235.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            34173..34781
                     /locus_tag="ACHA44_000926"
     CDS             34173..34781
                     /locus_tag="ACHA44_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8904236.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(34847..35020)
                     /locus_tag="ACHA44_000927"
     CDS             complement(34847..35020)
                     /locus_tag="ACHA44_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904237.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         9222 a         8600 c         9181 g         8111 t
ORIGIN      
        1 ccgtaaactg aaaaacaata aaagaaacgt tgaaagaata attattcctt caacgtttct
       61 tttttagaat gttttaatga ccaaagcagt ttcattgtca ggcttaggta attcatgaaa
      121 tcccgtattt atagtgctca attgatcaac taaccatgtt ttactttttg aattgtagat
      181 aaatctaaaa ttaatatcgt tattatcgtg ttgaaggggc gtctccaaat aatctgctgt
      241 atattcatag tcaacgtaac ctgctaatac aaaagagagt atccctttat ttaatgttag
      301 atttaatgga taatcatacg actttatttt tttcaacttc atattgtaca tatcttcagc
      361 cataaaagtt gagttcgtga tttcatcttg ggtctcatta aaaaagttat ccgaataatc
      421 gcttattttt gaaaagtctt tattggcacc agcctcaaaa aattgttcaa ctttttcagt
      481 ataaagttgt gctaacgctc ctttttcaat attaacttta atattttcat gtctatcaat
      541 ttgtgtgctg ttcagtaagg gaagatcagc ttttagtttt atatcgtctt gatttctttc
      601 aatttcaatt ggcaaaatct tttcagtaga ttcagtttct gataaaggaa catctagttt
      661 tttatcatga taataagctt caaatccatc tggaaccgga aaaaaatgta ttgggtaatt
      721 cttttctgag gttgttgtac tagtttccgt acttgtagaa cttgtgtcag aagtggtaca
      781 cccgcttata gttccgatga gtaaagcgca agttagaatg attgcaaatt tcttcaaaaa
      841 aattcctcct taaaattgaa tatggactaa tactagtccc attgtagcat accgaatctt
      901 taagattaat aagaaaaagg actaatttta gtccaaaagg tgtgctattc ttgaaagagg
      961 aaaaaattat tgataaagaa aatgtaatcg gtagcaatat tagacgagtc cgattagaaa
     1021 aaggaattgg tcaaaccgag ctgattagag acctacagct tcgtaaaatt gctataacta
     1081 gggaaacgtt ggttaagatt gaaggtggcc gccaacacat taagttggat caattgaaag
     1141 ccatcaaaga tattctacag gtgtcttatg aagatttgct tcaatgacta cttgtagtca
     1201 tttcgtttga aattattatg ttacaccctt atgtgtcacg aagaaatact ggtataataa
     1261 tgaatgggat atgtaacgat agggtatact gttatgttgc ctaataaatt gtggatcaaa
     1321 atacaagaat ggagggatat ccttgtataa ggcatatgaa ttggatttta caaatgctga
     1381 cttaaatgca ttctcctcaa gctcgcaatc atatacacat gtagtaaatc aaatagatca
     1441 caaccaaaaa tctattaaca agagaataga aaatctattt gaaagtgaaa atgatttagt
     1501 tgattattat tctaaggaca gcaaaattct agatgcagac gaaattatta aagattggtt
     1561 tccaactata aaagcagata tttttatatc tcactctcat gctgatgaaa aattagctat
     1621 acgttttgca tcatggctat ttgaaaattt tggtcttact gcatttattg attcttcggt
     1681 ttggggatac agttctgatt tattaaaaaa aatagaccaa aaatattgct ataaggaaca
     1741 aacaaaaaca tatgattatg ataaaagaaa tgtaacaact agtcatgttc atatgatgct
     1801 ttctacagct ttaaataata tgattgatag tacagaatgt ctgttttttc ttaatacacc
     1861 taattcaatt agtttgagca atgaaatcac taatgaacag aaatttacgt attcgccatg
     1921 gctatatagc gaactgacga cagcttcaat agtggaaaaa aagaatccac gattggaaag
     1981 taatcctcaa atgagcaccg aggatgttag aagcattata aagcactatt cttcgcaaga
     2041 aaatctgcaa ataaaatatt ctttaaattt aagtgaatta acttatttac gaaaagctga
     2101 tttgaaaaaa tgggctttag atgctgtgga atgcactaaa ggaagtaata cattagatat
     2161 tttatataaa aacaccattt tcactaatct ctaatcaatt tactgaatcg aaaggaaccg
     2221 ggtagaatgg ataagaaagt tttaataaag aggttctctg aagctattga aaaaggaaat
     2281 gcagcaatat ttgcaggtgc aggattatcg atgtcacaag gatatgtaag ttggccggaa
     2341 ctacttaatg atcctgcaac agaaatcggg ttggattcta aaaaagaaac agacttagtt
     2401 actctggctc aatattataa aaatgaaaat ggtggatcaa gaggaatttt gaatcagata
     2461 cttatggata attttggtga agaattagaa atatctgaaa atcatcgaat actagcaagt
     2521 ttacctattg aaacttattg gacaactaat tatgatcatc taatagaaaa atcaatccga
     2581 gaagcttata aaaaccctca ggttaagaaa aattatactc agttagcaac aactaatcca
     2641 aatgtggata caattgttta taaaatgcat ggtgatatag atgatgtcag ttcgacagta
     2701 ataacaagag atgattatga aaaatatgat gattcgtacg cgttatttaa agaaacttta
     2761 aaaggagatt tgttaacaaa aacttttctc tttttaggat ttagttttac agaccctaat
     2821 ttagaaagaa ttttatcaga tataagatgg gttttgagag aaaaccaaag acctcattat
     2881 tgtataatga gaaaaatatt atagaaaaaa ttttaggtta tcgctgaaag ccccggaaat
     2941 acgggcattt cgcaggatat gagaactcaa tttactacca atttactact ttcggattga
     3001 gccgcatata tgctgaacca tactgctaat acccgccacc ccaaatacgg cacatcataa
     3061 attgaatacg acaccgtaaa cgcggcgttc ctctttcccg tcatgggaga acaggacgcc
     3121 gcttttttat gcccaaatag agaaaggagg cgacgccatg ctatttactt ccggccctga
     3181 ccgggcgttt gaacggctga tgcaggaaaa gcccggtttc gatcactacg acggcggcgg
     3241 ggcggacgcg ccggaggatt gcggcacctg ccgattttac cgcccggatt ggaaatatca
     3301 attctgccag tttgcagagt gtccctatca gccgggcaag ctgaccgact atgcagcggt
     3361 caaattcaca gtgaaaggag cgaacaacgc tatggcagtt ttccgtgtgg aaaagaacaa
     3421 aggctatacg gtgatgtcaa atcaccacct gcggaataag aacctgacct taaaggccaa
     3481 gggcctgctg tcgcagatgt tgtccttgcc ggaggattgg gactataccc taaaaggact
     3541 gtcctatatc aaccgggaaa gcatcgacgc gatcaggaca gcggtatggg agcttgaaaa
     3601 agctggatat atccggcgtg agcagggccg tgacgcaaaa ggcaaaatgg ccgatatggt
     3661 ctataccatc tacgaacagc cggtattgga aaacccggta ttggaaaatc caacatcgga
     3721 taacccggtg ttggaaaatc cgacatcgga taatccggcg tcggaaaatc caacgcaatt
     3781 aaataaagaa gtacaaagaa ctgacttatc aaaaaaagaa aaaataatta cagatggaca
     3841 aagtacccat tccattccta tcccatcccc gacccctgcc cctatggagg gcgaggcagc
     3901 ggctacgccg ccggaacgga aaaggacagg aaaggatgag gctgtacgga tttatcggga
     3961 aatcattttg gagaacatcg agtatgccta tctcattcag gacaggagca ttgaccgtga
     4021 acagcttgac gaaatcgtag acctgatgct ggaaaccgtc tgcaccgccc gaaagacaat
     4081 ccgtgtcgcc ggggacgact accctgccga gctggtgaaa tccaagtaca tgaagctgac
     4141 cggcgaacat atccgctttg tccttgactg tctgcgggag aacacaacga aagtccgcaa
     4201 tatcaaacaa tacctgcggg ctatgctgtt caacgccccc agcaccattt ccaactacta
     4261 cacggccctt gtcgcgcacg acatggcaca gcccgattgg ggaaagccta aatccggcct
     4321 gccggactat tcctgcggcg agggcgaaag cctgtaacga gaaaaggagg acgaccctat
     4381 gaagcagggc gcattgattt ttgacgagcg aacagaccgt tacgacatcc gctttgatct
     4441 ggccgactat tacggcggcc tgcattgcgg ggagtgtttc gacgtgatga tcggcggcaa
     4501 atggaggccc acccgcattg aaatggccga aaactggtat cttgtcggca tccgggccga
     4561 cgacctttcc ggcctgcggg tgcggatttg acctatatct acctgcggcg tctggtggtt
     4621 ccgccttaga cgccgctttt gactttctga caaggaggga tgcgattgca ggaagaagtt
     4681 actcaaaaga ccattgccct gtccatgaaa acgggcaagc tcacggctca agtgctgcaa
     4741 gcggcgctga aaaagtacct gcaacaccgg gccaagggca aaaacacgct gcaccacggc
     4801 cagcagagcc taaagcagct taaaaagcat ggggctgatc tctccaacat tgagatcacc
     4861 gaggccaaca ttggagcgtt caagccatgc gccaagaaat acggcgtgga ttttacctta
     4921 cgaaaagata agaccaccca gccgccccac tacgttgtga ttttcaaggc caaggatgca
     4981 gataaccttg atctggcgtt tcgggagttt accgccaaga cgctttccaa ggagcagcgg
     5041 ccctctatcc gcaaggtttt gtccgctgtg aagcaaaagg cggcggccca gaccaagcag
     5101 cgggccaagg agaaaatcaa ggaaaggggg ctggaactgt gaagcctgac gtaaagaaat
     5161 tgctgatcct gaacctcccg tatctgattt ttgtgtacct gtttgcgaaa tgcggggagg
     5221 cgtaccggct ggccgctggt gctgacctgt cggggaagct cctgcatttt gcggatggtt
     5281 ttgcggcggc ttttgccaac cctctgccaa gcctgcacct gtttgacctg tgtatcggta
     5341 tcgccggtgc cgtgatcgtg cggctggtgg tgtactgcaa gagcaagaac gtgaagaaat
     5401 accgcaaagg catggagtat ggcagcgccc gctgggggac ggccaaagac atagcgcctt
     5461 acatcgaccc caagtttgaa aataatatcc tgctcaccca aacggaacgg cttaccatga
     5521 ccggcaggcc caaagacccc aagacagcgc ggaataaaaa cgtgctggtg atcggtggca
     5581 gcggttcagg gaaaacgaga tttttcgtga aacccaacct gatgcaatgc tttccgacca
     5641 cggactatcc gacttcgttt gtggtgacag atccgaaagg cactttggtt ctggaaaccg
     5701 gcaggatgtt ccagcaggcg ggctaccgca ttaaaatcct gaataccatt aacttttcca
     5761 agtccatgaa gtacaacccc tttgtctaca tccattcgga aaaggacgtg ctaaagctgg
     5821 taaataccct gatttccaac accaagggcg agggggaaaa gtcagcggag gatttttggg
     5881 tgaagtcgga acggttgttt tattcggccc tgatcggcta tatctggtac gaggccccag
     5941 cggaggaaat gaacttctca acgctgctgg aaatgataaa cgccagtgaa gcccgcgagg
     6001 acgacccgga gtttcaaagc cccgtcgatc tcatgtttga gcgtttggag cagaaagacc
     6061 ccgaccattt tgcggtgcgc cagtacaaga aattcctgct gtccgcaggc aagacccgtt
     6121 cttccatcct gatttcatgc ggtgccagac ttgcgccctt tgacatccgg gaggtacgcg
     6181 agctgatgga ggacgacgaa atggagctgg acaccatcgg ggacgaaaag acggtgctgt
     6241 atctcattat gagcgatacg gacacgacct ttaatttcat tctggctatg ctccaaagcc
     6301 agctaatcaa cctgctttgt gaccgggctg atgatgtgta cggcgggcgg ctgcctgtcc
     6361 atgtacgcct gattttggat gaatttgcca acattggcca aatacccaat ttcgataaac
     6421 tcattgccac cattcgaagc cgtgaaatat cggcctctat tattttgcag agccagtcgc
     6481 agctaaaagc gatttacaag gacgccgccg agatcatttc ggataactgc gatagtgtgc
     6541 ttttccttag tgggcgcggc aaaaatgcca aggagatttc cgatgcgctg gggaaagaaa
     6601 cgattgacag tttcaacacc agcgagaacc ggggaagcca aacgtcccac ggattgaact
     6661 atcaaaaatt aggaaaggag ttgatgtccc aagacgaaat tgccgtgatg gacggcggca
     6721 agtgtatctt gcagctacgg ggtgtgcgcc cgtttttatc ggagaaattc gatattacca
     6781 agcatccccg gtacaaatac cttgccgacg ccgacaagaa gaataccttt gacgtggaca
     6841 gatttttgac tatcacgcgc cggaaacggc agcaagtggt aacgcaggat gaagtattcg
     6901 acctgtatga aatcgacctg tcggacgagg acgccgccgc tgaataaaca gcggcttttt
     6961 tcattccaac aacaaaatca aaaaatcatt atgagccgga aaccggcaga aagaggtaaa
     7021 tttatggcat tttttaattc cgcagttact actttgcaga ctatcgtgat cggactgggc
     7081 ggcgctctgt gcgtatgggg cggcatcaat ctgttggagg gatacgggca ggacaatccg
     7141 ggggcaaaat cgcagggtgt caagcagctt gtcgctggcg gcggcgtggc cctgatcggt
     7201 gtcactctgg ttcccctgct gtccgggctg ctgggctgat cctggccttt gtccctgtcc
     7261 aaacccttaa ctaatcgcct ccgcgtcctc ccggccagac cgggagggcg cgggaaagga
     7321 ggtgctaccc gtgattagtg aaatcattga agaatggata aaaggcatcc tcatggacgg
     7381 tatcacgggc aacctgtccg ggctgttcga tacggtaaat gccaaagtcg gagaaatcgc
     7441 cgccgacgtg ggacgaaccc cgcaggcatg gaacagcggt attttcaata tgctgcacag
     7501 cctgtcggaa accgtgatcg tccccatagc cggggccata ctcgcccttg tcatgtgcta
     7561 tgagcttatc cagatgataa cggaaaagaa caatatgcac gactttgaca gctccatgtt
     7621 cttccgctgg atattcaaat ccgcgtttgc aatcctgatc gtaacgaaca catggaacat
     7681 cgtcatgggc gtattcgacg ccacccaaag cgtcgtcaat caaagcgccg gtgtgattat
     7741 cgggcagaca tccattgatt ttaacaccct gataccagac cttgaaagcc aattggaggc
     7801 tatgagcgtg ggcgctcttt tggggctgtg gttccaaacc cttgttgtgg gcctcaccat
     7861 gaatatcctt tccatctgca ttttccttgt gacctatggc cgcatggtgg aaatctatgt
     7921 tgtcacgtcg ctggggccta tcccccttgc cacaatgggc aacgcggaat ggcgcggcat
     7981 gggccaaggg tatctgaaat ccctgctggc gctgggcttc caagcctttt taatcatggt
     8041 tgtggtgggt atctacgcgg tgctgataca gaatatcggt tccgcttccg acatatccgg
     8101 cgcaatctgg ggctgtatgg gctatacggt gctgctgtgc ttctgcctgt tcaagaccgg
     8161 cagtatatca aaggccgtat tcggggcgca ttgatcccaa aatccaagca tacacaggag
     8221 gaacttgttt atttcaacgt acaacatcat ttacgctgac ccgccgtggc ggtatgactg
     8281 caaaaacgta cagggcgcag cggaaaatca ctattccacc atgagcattg acgagctgtg
     8341 cgccctgccg gtggaacggc tggcgtccaa agactgcctg ctgtttttgt gggccacatt
     8401 ccccatgctg ccggaagctc tgcggttaat caaagcgtgg ggcttttcat tcaagaccgt
     8461 ggcttttgta tggctgaaac agaaccgtaa aagccttaca tggttttacg ggctgggccg
     8521 ctggacaagg ggaaacgcgg aaatctgcct gctggcaaca cacggcaagc cgaagcgtcg
     8581 ttctgcctct gtccatcagt ttattatcag cccgattgaa caacacagca aaaagccgga
     8641 tgtgacccgt gaaaaaatcg tagagctggc gggcgatttg ccccgcgtgg agctgtttgc
     8701 cagacagaaa acccccggct gggacgtgtg ggggaacgag gtggaatgtg accttatcct
     8761 gacgtgaaag gaggtgccaa atggcttatg taaccgtccc caaggactta acccatgtga
     8821 aaagcaaggt tgtgtttggg cttaccaaac ggcaactgat ctgtttttcc ggggcgctgg
     8881 tgatcggcgt ccccttatat tttctcaccc gtgactatct taccaacagc gcggcggccc
     8941 tgctcatggt atttgccatg ctgccgggct ttttgcttgc catgtacgag cgtcacggcc
     9001 agccccttga agtggtgatt atgcagatca tccagtgctg cttcatccag ccaaaggagc
     9061 gtccctatca gaccgacaac acctacaccg cccttgtgcg gcaataccag atggaacagg
     9121 aggtaaaggc cattgtccaa aaaggaaatc ccaagagaaa ccgaaaaaac aaagctgacc
     9181 cgcgcccagc gaaaagaaat tgacgcggta atccgtaaat ataagggcga cggcaggccc
     9241 cacacggcgc agcagagcat cccctacgag gtcatgtacc cggacggcgt gtgccgggta
     9301 tcgcccggcg tcttttccaa gtgcattgag tttgcggaca tcagctatca gcttgcccag
     9361 ccggacacgc agacggctat ctttgaaaaa ctgtgcgacc tgtataacta tgtggacgcc
     9421 tccattcata tccagttttc tttcctcaat cggaaagttg acccggtgca gtacgccaag
     9481 agctttgaga tcgcgccgca gggggacgac ttcgacgaca tccgggccga gtacaccggc
     9541 attctgcaaa agcagcttgc aaacggcaac aacggcatgg taaagaccaa atacctgaca
     9601 ttcaccatcg aggccgaaag cgtgaaagcg gcgcgagcca gattgaagcg catcggcttt
     9661 gacctgttag gctatttcaa gagcatgggg gccgtagccc atgtgatgga cggctgggaa
     9721 cggctgaacc tgctgcatgg cgtgtaccac ccggacggcg agatattcaa ttttgactgg
     9781 aagtggctgg ccccgtccgg cctctccacc aaggatttta ttgccccgtc ctccctgtgc
     9841 ttcggcaatg caaagacatt cggcatgggc ggcaaatacg gcgcggtaag tttcctgcaa
     9901 atcctgtccc cggagctgtc ggacgatatg ctggccgact tcctcaatac ggaaagcggt
     9961 gttttagtca atctccatgt gcaggccatt gaacagacca aggcgatcaa gaccatcaag
    10021 cgcaaaatta ccgaccttga cgctatgaag attgcggagc agaaaaaggc cgtccgcagc
    10081 ggttacgata tggacattct tcccagcgac cttgccacct acggcgagga cgccaagaag
    10141 ctgctgacaa agttgcagac ccgcaatgaa cggctgttcc agttgacgtt ccttgtcctc
    10201 aacgtggccg acaccaagca aaagctgaat aacgacgtgt tccaagccgc aggcgtggcc
    10261 caaaagcata actgtcccct tgtccggctg gactaccagc aggaacaggg ccttgcaagc
    10321 agcctgccgc tgggcgtcaa tcagatcaag atacagcgca gccttacgac ttccagcgtg
    10381 gccgtgttcg tcccctttgt gacgcaggag ctttttcagg gcggcgcggc catgtattac
    10441 ggcatcaacg ccaagtcccg caacatgatt atgctggaca ggaagcaggc ccgttgcccc
    10501 aatgccttga agctcggcac ccccggcagc ggcaaatcca tgagctgtaa atccgagatc
    10561 gtcagtgtgt tcttaacgac gcctgacgat atttttattt cagacccgga ggccgaatat
    10621 tacccccttg tcaaacggct ccacggacag gtaatccggc tgtcgcctac cagcaaggac
    10681 tttgtgaacc cgctggacat caacctgaat tattcggagg acgataaccc gctggccctg
    10741 aaatccgact ttgtgctgtc gttctgtgag cttgtcatgg gcggcaagaa cgggctggaa
    10801 gccattgaaa agaccgtgat agaccgggct gtgcgcgtga tctaccggcc ctatctggca
    10861 gacccccgcc cggagaatat gccgatcctg tccgacctcc acaaagcgtt gttagatcag
    10921 catgtcccgg aggctgaccg ggtggcgcag gcccttgacc tgtatgtgtc cggctccctg
    10981 aacgtattta accaccgtac caacgtggac atcggcaacc ggcttgtgtc ctttgacatt
    11041 aaggagctgg gcaagcagct caaaaagctg gggatgctca ttgtccaaga ccagatatgg
    11101 gggcgcgtca cggccaaccg aagccaagga aaggccacat ggtattttgc cgacgagttc
    11161 catttgcttt tgaaagagga acagacggcg gcctattctg cggagatttg gaagcggttt
    11221 aggaaatggg gcggcattcc aacaggtgcc acccagaacg tcaaagacct gctttcctcc
    11281 ccggaaattg aaaatatcct tgaaaacagc gacttcatta cgctgctcaa tcaggcgtcc
    11341 ggggatagaa aaatccttgc ggaacgcttg aatttgtcca ccgaacagca gaagtacatc
    11401 gacaattccg aacccggcga gggccttttg atctttgaaa acgtggtgct gccctttacc
    11461 aaccccatcc cgcacaacac ccagctttac aagattatga cgacccgctt aaacgaggtg
    11521 gcgggagtat gacccggcga cgccacaaag gaggtgaacc accatgaaag agctgaaagc
    11581 caaggcgaaa gtgacgcagg ccatgacccg cgacgggctg acgctggaaa atcaggccac
    11641 cggcgaggtg gagaacattt ccagccggga ggccgaacag gatttgtccg cgccggggcc
    11701 gggcggcacc gccgataagc tgctggaacg ggccgacgcc atccatgacg gccataaggc
    11761 caagaaagcc gcgaagcagg ccggggctgt ggccgccgag ggggacgctg ccctgcaccg
    11821 gccatccgcc cggctccaac tgtcggagga agaacgggcc gacccggatt tacagcccta
    11881 tatccgaaaa tcggacaaac gggccgataa gctggataag gcccgcgccg ccatccccaa
    11941 gaagcgcgtc ccggtcaagg aaaccgtctt tgacgaggca agcggcaaag caaagaccgt
    12001 cctgcacttt gacaaacagg acaaggggcc tccctccctg aaacccaatc cggcaggccg
    12061 tcctgtttcc gaggtttttc tattcggcca cgggaaaatc catgaagtcg agcatgaaaa
    12121 cgtgggtgtg gagggcggcc ataaggggga ggaacttgcc gagcggcagg ccggaaaagc
    12181 catccgcagc accatccggc accacaggct gaaaccctac tgggacgcgg aaaaggccga
    12241 gcgcaggcta ttgtctgcca acgcggagta tttctaccag aaatctttgc gggataaccc
    12301 ggagatcgca caggcggcca gcaaccccat ttcccggttc tggcagaaac agcagatcaa
    12361 gcgccagtac gccaaggccg cccggacagc gggccagacc gcaggcgcgg cggcacaggg
    12421 cgcggccaag acagccgggg cctccacgaa agcggccaag gccgcagcca aagaaagtga
    12481 acgggcggcc tcctttgtgg cccgccactg gaaaggggcg ctggccgtcc ttgtgttggg
    12541 gcttttgatg ctgcttatca tcggcggctt acagtcctgc accgccatgt tcggcagcgc
    12601 cggaagcggc atcacggcgt cctcttactt ttccgaggat agcgatatgt tggccgccga
    12661 ggacgcctac gcggagctgg aagccgagct gcaagactat ctggacaact acgaggccac
    12721 ccatgactat gacgaatacc attttgacct tgacgagatc agccatgacc cctatgtgct
    12781 gatttccatt ctgtcggcgc tccatgacgg gatatttacc atcggggagg tacagggtga
    12841 tttgtccatg ctctttgaca agcaatatat cctgacagaa aacgtcgtgg tagaaacccg
    12901 ttaccgcacc gaaaccgata catggacgga cgcggagggc aacacccata cagacaccta
    12961 ccaagtgccg tatgactatt acatctgcta cgtcacgctg gaaaacttcg acctgtccca
    13021 tgtgccagtc tatatcatgg acgaggaaac gctaagtttc tattccctgt atatgtccac
    13081 gctaggaaac cggcctgatc tgttttccgg cagccagtac cccaacgcct ccaccctgac
    13141 gccgccgacc tactacgacg tacccccgga ggctttggag gatgccacct ttgccgccat
    13201 gctcaaagag gcggaaaaat atatcggtta tccctatgtg tggggcggca gctctcccag
    13261 caccagcttt gattgcagcg gcttcatttc gtgggtaatc aatcatagtg gatggaacgt
    13321 gggacggctg ggggcgcagg gcctatataa catctgcacc cctgtttcca gttcgcaggc
    13381 caagcccggc gaccttgtat ttttcaaggg aacctacgac acccccggcg tgtcccacgt
    13441 cggcctgtat gtgggcaaca atgtgatgct gcattgcggg aacccgattt cctacacgaa
    13501 ccttacttca aactactggc aatcccattt ttacagttac gggcgtttac cttaacgcca
    13561 gaaaggagct ttcaattatg gcaaccaaac ttgaccgcat cgaaaaggac atccagaaaa
    13621 ccaagtctaa gatcgcggag tttcagaaac agcttcggga gctggaaacg cagaaaaccg
    13681 aacaggagaa tttgcagatc atccagcttg tgcgcggcat gaacatgaag ccggaggaat
    13741 ttgccgcgtt cttacgcagc ggcgcaatgc aggccgcccc tgccgcaacc ccttaccatg
    13801 aacaggagga caatgcccat gaagaataag accagacgga tttcccgtat tctccccgca
    13861 cttctggccg tgatgctctg cgtcacggcc tttcccgtta ccgcccttgc aggcggcaat
    13921 gacccggcac cggccccgct gccggaggct acaaccgaac ccgctacggg cggtttggag
    13981 cctgaaacag acgaaaccgg caccgccacg gacgggacag aaaccgaggc cgaaaaagat
    14041 accggccctc tgaccggcaa ggacatcacc gacctgtttt ccgcgctgtt cggttccaag
    14101 gtgagcattg ccgccacgga cgacggcatc cagatcacca ccggcaccga caaaaaagaa
    14161 cctgaacaga ccggcaccgt caccaccaac ggcggcaggc tgaatgtccg caccggcgcg
    14221 gggcttgaca agaccgcgtt cacccagctt cccaatggga cgacggttga ggtagtcggc
    14281 acagacggcg actggattaa aattctcttg ccggagcgca tcggctatgt ccattccgac
    14341 tatatgaccg tcagcgaaaa ggaagtagcg gctaccggcg agggcggttt ttccctctcc
    14401 attgacccgg aggaaattgc ttctctgctg gaactgttca atggcggcgg ggccagcgcc
    14461 gccctgtccc cggacgggaa cctgtcactg attgacgaca tcggcagcgc cgcgaaaaat
    14521 ggcaagcagt ttatcaccgt ggaaaccaaa aacggcaatg tgttttatct gattattgac
    14581 cgggacgacg agggaaaaga aactgtccat ttcttaaatc aggtggatga agccgatctg
    14641 ctgacgctga tggaggacgg ggaaaccgtc acccccgctg ctgtctgctc ctgtaccacg
    14701 aaatgcaagg ccggggctgt gaacacgaat tgcccggtat gtaaaaccaa cctgaccgag
    14761 tgcagcggcc cggagccgca ggaaccccag ccggaggaac ccgaagcccc ggaggaagaa
    14821 cccaagggcg gcgcgggcgg cctgatcgtg ttcctgctgg tggcgctggc gggcggcggt
    14881 gcggcccttt actatttcaa gttcaaaaag cccaaggctg aaaccaccgg cagcgacgat
    14941 ttgggcgagt atgattttgg ggaggacgag gatttggagg acgaggaacc cgaaaccgag
    15001 tttgacccgg aagctgattt tgtgagtgaa caggaggacg aggaatgaca tataaacttg
    15061 taatcgcgga aaaacccagc gtggcccgca gcattgcggg cgtgatcggg gccacggaga
    15121 aacacgacgg ctatttggag gggaacggct atctggtgag ctggtgcatc ggacatctgg
    15181 tgtcctttgc cgacgccgga cgctatgacg agcgtttcaa aaaatggcgc tatgaggatt
    15241 tgcccattct cccggaaaca tggcagtaca tcgtccccga cgacaagaaa cagcagtttg
    15301 acgttctctg ttcgctgatg cagcgccccg acgtgaccgg ccttgtgtgc gccaccgacg
    15361 ccgggcgcga gggggaattg attttccgtt tcgtctacca gatggcggga tgccaaaagc
    15421 cctttgagcg cctttggatt tcctctatgg aggacagtgc tattaaggac ggctttgccc
    15481 acctgaaacc gggcggcgac tatgatccgc tgtatcaatc ggccctgtgc cgggccaagg
    15541 ccgactggct gatcggcatt aacgccaccc ggcttttttc cgtcctgtac cacaagaacc
    15601 tgactgtggg ccgcgtccag acgccgacct taaaaatgct ggtggacagg gacgccaaga
    15661 ttaccgattt caagaaagag aaatatcaca tcgtccatat cacggcgggc ggcgcggacg
    15721 cggtaagcag ccgcttttcc gacgctgccg aggcaaacac cgtcaaggcg gcttgtgcaa
    15781 aagcgcaggc cgtttgcgtt tccgtgacgc gggagaaaaa gacggaacag ccgcccaagc
    15841 tctatgacct taccaccttg cagcgggagg ctaaccggct tttcggctat accgccaagc
    15901 agacccttga ctatgcccag gcactgtatc taaagaagct gctgacctat ccgagaacgg
    15961 atagtcaata tcttaccgac gatatgctgc ccgccgtgga aagccttgtg tccggcctgt
    16021 gggagctggt gccgtttgcc aagggcctga acctttcccc gcagtttgac cgcatcctga
    16081 acagcaaaaa ggtgtccgac caccacgcca ttatccccac cgctgaattt gtgaagcagg
    16141 gctttggcac tctggccgag agtgaaaaaa agctgctgtc cctgatctgc tgcaagctct
    16201 tatgtgccgt ggccgggccg catatctatg aggccgtcac cgccacattt acctgtgccg
    16261 ggcaggagtt taccgccaag ggcaagaccg tcctttcccc cggctggaaa gagattgaac
    16321 gccggttccg ttcttcctta aagaccgacg ccgacgagga cgccgaggcg gtgcgggagc
    16381 tgccggagct ttgcgagggc cagacctttg cagacgtggc cgcctctgtc accgagcatt
    16441 tcaccacgcc gcccaagccc tacaccgaag atacgctttt gtccgctatg gaacgggccg
    16501 gggccgagga tatgccggag gatgccgagc gcaagggatt aggcacaccg gccacccgcg
    16561 ccgccattct ggaaaagctg gtgcagatgg gctttgtcca gagaaaaggc aagcagctca
    16621 ttcccaccaa ggacggcatc aatctggctg tggtgctgcc ggaggcgctt acttctcccc
    16681 agcttactgc cgaatgggaa aaccggctga ccgagatcgc caaggggcag gccgatcctg
    16741 acgaatttat ggacggcatc gagacgcagg cgcgggagct ggtgcagacc tattcctgta
    16801 tcagtgagga aaagcagaaa ttgtttcagg ccgagcgtgt tcctatcggg acgtgtcccc
    16861 gctgtggtga ggctgtctat gagggcaaga aaaactacta ctgcggaaac cgggcctgcc
    16921 agttcgtcat gtggaagaat gaccgatttt ttgaggaacg caagaaagcg ttcaccccga
    16981 agatcgccgc tgcgctgctc aaaaccggca aagtcaaggt aaagggcctc tattccgtaa
    17041 agaccggcaa aacctacgac gggacggtgc ttttggctga taccgggggc aagtacgtca
    17101 actaccgcat tgaacacagg agctaaagga cagtaacaca gcaagcatag ggagtgtggc
    17161 tacactcccg gtaaaatccc ccggccgcgt ccctgccggg ccggggattt ttgcttgttg
    17221 gggaagtccc caacccccta ttttttgaaa cagaacagag aaaggagtga ccgcgtgaac
    17281 gaagtatttt ctatccaagt ccataaccgg cagtttttgg aacagggcaa acaaggcgtc
    17341 tggctggatt tgcccaccac aaccgaaacg ctgcaagccg ccctgcggga tgttggcatt
    17401 actgccgaca atccgcagga tttttttatc aacgggtatt cctgcccgga ggacagacac
    17461 cttgccattc cctatgacat ggtgctggcc gccgacgtgg acgagctgaa tttccttgcc
    17521 gcccggctgg gccagcttga cgccgccgag atcgcagagc tgaacgccgc cctgcaaaat
    17581 ccccgtggcg gctttgagaa tatcggccag atcatcgact acaccgaaaa tgtggattac
    17641 tatgtgcatc ttcccgacgt taggggggat ggacagttgg gcgactacta cctgaaccgt
    17701 tccggcatgg tggatatgcc ggaggaatgg aaagccggga ttgatacggc gcagtttgga
    17761 cgccacattg cccagcagga acaaggcgtt tttacccagt acggctatct ggtgagaagc
    17821 ggcgacgaat ggcagcgcgt ccatgagggc cagccggtgc cggaggaata ccgggtgatg
    17881 cactaccccc agccggaagc tgaccgggag gaaccgcagg cccagcagcc ggaggcatcc
    17941 gaaagccccg acaaagcgcc gcccccggtt atcccgatca tgctggacgg catgaacagc
    18001 aaagaacgca tgaaagtgat tactgaccgt ctggaaaccg gcgtacagga gctttttgaa
    18061 agtagccgct atatggctta cttatccgct atgtccaaga tgcacgatta cagtctgaac
    18121 aatacgctgc tgatcgccat gcagggcggt tcgattgtgg cgggctataa caggtggcag
    18181 gatgaatttg accgccatgt gaagcggggc gagaaaggca ttaagatact ggccccggcc
    18241 ccgttcaagg ttaagcagca gatggagaaa accgacccgg ccacccagca gcccgtgatc
    18301 ggcgcggacg gcaagcccgt gaccgaagaa caggaagtga cggttccggc ctttaaggtg
    18361 gtgacagtct ttgacatcag ccagaccgag ggcaaggatt ttcccatgcc ctatatcagt
    18421 gaacttaccg gcgacgtgca gcagtatcag gatttctttg ccgcgctgga acggacatca
    18481 ccctatccca taagcattga gccgatttca gacggggcca agggccgctg cttctacgca
    18541 aaacagcgca ttgccatcaa cgagggcatg agcgaattgc agaccctaaa gaccgccatc
    18601 catgagattg cccatgccaa gctccacgcc attgaccggg aggcccccgc cgaggaacag
    18661 gcggcccggc cagaccgcag cacccgcgag gtacaggccg aaagcgtggc ctataccgtc
    18721 tgccagtatt acgggctgga cacgtcggac tattcttttg gctatgtggc cgattggagc
    18781 agcggcaggg agttatccga gctgaaagcc tcccttgaaa ccatccgggc cacggccagc
    18841 gaattgataa ccgagatcga cggccatttt gcccagcttc agcaggagcg cgaggcccgg
    18901 caggaacagg aggccgtcac ggttgacgtt accccggagg cagcggcccc ggcgcaggcc
    18961 gaggaagaac aggccccggc ccttgatccg gcagccgagc cgcttgtgac tgtgctatgg
    19021 agcgaaagcc cccagctaaa ggacggccag caaatgccgt tgcatgaggc cgacgcgctg
    19081 tttgcccgtc tggatgcagc atatccggca gaggcaggct atgacaaaac cgcgttccgc
    19141 attgactgta tctttcaggg cgagccggac agctacgagg ggcggcagga ttttggagat
    19201 agggacggtt ccctgatcga acatatccag agctaccacc aattttacgc gcaggacgaa
    19261 aactggaaaa agcatgtaat cgctacaacc ggcatagagg gctgggagca ggacaaggcc
    19321 catcgggata tgctgttagg cgagtttgtc ccctatatga agctgcattg cagccttgcc
    19381 caaatggaac agacggcgca gggccgctta cagtccgggg aaaccctgac gccggaggaa
    19441 acggcctatt tgggggcgct tctctcttat gtgcaggagt gccgccccat gctcaatcag
    19501 ggccagtatc agttgccgga gccgccgaaa ctggctgact tcgaccagag cttgcaggac
    19561 tacaaagcac aggtacaggc cgagatcgcg caggaggccg ccgcagccgg tatgaccgtg
    19621 gaggaatacg cggcggctgg ctatgaagca ccccaacagg acaccttttc catctaccag
    19681 cttcgaggcg gggaggaaac ccgcgactat cggtttgagc catacgagcg tttacaggcc
    19741 gcagggctgg ccgtggacag tgccaactat gaccttgttt atacggcacc gctggataaa
    19801 agcacgtctt tggaggatat ttaccgcaca ttcaacatcg accacccggc agattttagg
    19861 ggacacagcc tttccgtttc cgacgtggtg gtgctgcacc agaatggaca ggacaccgcc
    19921 cactatgtag acagcttcgg ctatatggaa gtgccggagt ttttgcggga aaatcccctg
    19981 cgtaccgccg aactgtctac ggagcagaac gaaaacatga ttgacggcat tatcaacaat
    20041 acgccgcccg aaccgcagcc ggaggaaccc gaacagctat cttttgagga tgtggcagtt
    20101 gcttccggcc agacacagga ggaacccccg gaggctgcgc ccccgcaaac ggctgtccgc
    20161 tattatccca tcaatgaggg agccgcccgc cgcgccaagg aagccatgag cttttctgac
    20221 taccggcccg gcagcgccac ggcggaatac cggcactatg tcgatcaggc cgtggaaatt
    20281 gcggagcgcc agaaaaagcg tgtggagcca gagtatcacg aaaagataga cagcctcctt
    20341 gacacctacg cccgcaagct ggccgagaac atgaataagg gctatgagat taccgcccgc
    20401 gtcccctctg tcctgattgc cgggccgtcc aatttcccgg tacgggcgaa agaaaagcag
    20461 aacgccgcca gtgaccgcaa catggaggaa tttcagtaca ttcagggctt gcttgacaaa
    20521 atccgcagca ccggcatggg tggtatcagc gccgacgatc cgcaggccgt ttccaagctg
    20581 gaaaagaaac tggaaaagct ggaagcgtcg caggagttga tgaaggccgt caatgactac
    20641 taccgcaagc atgggacgct ggacggatgc ccccatctga tcgaaagagg cattgaaaac
    20701 ctgaaagcgg atatggcgtc gggctggcac tatgagaaaa aaccgttcca gtcctggcag
    20761 ctctccaaca ataacgccga gatacgccgg ttaaagggcc gcattgagga attgacccgc
    20821 caaaaggagg cggcctatgt gggatgggag tttgacggcg gcactgtgga gatcaaccgg
    20881 gtggccaacc gcctgcaaat cttctttgag gggaagccgg atgctgctgt acgcgacgag
    20941 ctgaaaagca acggtttccg ctggtcgccc aaggccgggg catggcagcg gcagttaaac
    21001 gacaccacaa tccgcgtcgc tgaccgtatc aagtgcatcc agcccctttc cggcgaaaag
    21061 cccagcgcct tacagcttgc ggcccgcagg gagaaagaaa agccctctat ccgggcgcag
    21121 ctcaacgcgg ccaagaccga acaggccaag cagccaccgg cgcgggaaaa atcaaaggga
    21181 ttggaggtag agtaaatgca gaagtttgaa aatgtggacg ttctggccgc gctggaacag
    21241 atcatgcggc agaacaccgc gttttatcaa agtgactttg gcattgacaa agacattatc
    21301 aggcgggatg ccgccagcga tcaggccgca gacaagaccc tgctctggat gtcccggccc
    21361 tccgggacgt actgtttccg ggaacgtgat gttttcctga aagacacccg acagtacaac
    21421 acatggaaat tttacgggga gcagacccgt gacaagatac tggcctacgc ggtggagctg
    21481 accggcacac agggcggcac tatccggggc aatctctatg agctggacta tccgcagcat
    21541 ttccggcacg tcattgaaac cgcccagccg gtgagtgtga accggctctt ttatgagcat
    21601 ggcacccgtg acatcccgga gagccagcgt tttgacggaa gccctgaccg ggtattggga
    21661 aactttttgt actatgaggc ccagccccat gacccggctg tgctgcagga ggcgctacgg
    21721 acggagcagc acagccgggg gcgggccgtc cccggcgatt tcaaagtcca cgtcaccgct
    21781 ttgcatgaca gccggattga aaccgaggcc cggcgtattg tggacagcct gaaagatctg
    21841 tccgcgccta acagccccaa taaaacgcat ttcatggtgg agatctcccc gcactttgcg
    21901 gcgattgctt ccaccaagga taatgaccgg ctctttgcca tgctgcccta taaatccctg
    21961 tgttttaccg ggatgaaagg caggcatggc gtgtatgccg tgattaaccg ggacgagcgc
    22021 cgggatgtga gtattcgcag gccccggccc tctatccgca agcagctatc cgacagcaaa
    22081 caggccgcca gcccaaaaaa ggcggcggcc agaaccaaaa agaatgaatt ggaggtataa
    22141 ggaatgatgc actttaccgt ggaggaagaa aacctgatct gtatgtacca caatgcagac
    22201 cggcgcagaa cgataaacag gctcaccgcc gtcatgccgg ttatggatgg ggatatgcgg
    22261 gcgcaggccc agcagaccct ttccaagctg gaacgcatga cggatgccga ctatgacggc
    22321 cagaagttcc acttcacaga cgaataacga acagcagacg ccgggcgcgg cggccatgtg
    22381 gtcgcttgcg tccggcgtct tttctttttg cctattccta cgcatttaga acccggtatt
    22441 gtcggggata ggatgaagta ttcgcttgcc cctgcaaaca tgcgggagaa gccttgcagc
    22501 gggcggtttt caatccctta aatgcgtagg ttgcagctat aattagaccc ctgaaacaga
    22561 tatagccgcg cagcgggtgt atctgtttca ggggtgtgca ggtgatagcc gcaaagcggc
    22621 gtaagagggt gcagccctct tgccgtctat ctctgttcca gcgggaagct cggcaatgct
    22681 tccagcagtt tcagggaaat gcgctgccgt atatcttcgt caatgacttt cttctttttg
    22741 ccgtttgcct gtctgatctc caccgtggaa agctcgttga tgtagtcagc ataatgggcc
    22801 aacacttttt ccgtggccca cctctcaccg gcaacggcgg ctttgatcgt ttcatagtcc
    22861 agcagctttt cttgtccgtc catcctgata cccccgtcag actgattttt tctatgatac
    22921 catattcgga agcatggaac cagagaaaac ggcgcttttc tgctggtaaa ttctacgcat
    22981 ttagaacagc acttcggcgg ggataggata aaacacccac ataccccgct gaccatgccg
    23041 gaaaagcctt gaaatataag ggcttttaca gctctaagtg cgtaggtttc cagtatcttt
    23101 tccgctggcg ctcggcctct ttgcggcggc gcatccgttt ggagcaggcc gcgcagtatt
    23161 tggcccggtt agacttcggc gtgaacgtgg ccccgcactc acagcagcgt ttcacttcgt
    23221 cccggtcttt ggtgatctcg gcatacaggg ccgcatccag cgggaggaca gcggccttaa
    23281 accagcggca cagaagcgaa taggaaatgc tctgtacgca aatacattct tcgccgtcgt
    23341 ccagcaacag gcagttgccg tcacaatagt tgcagcactc atgcgtcagc ttccgggcct
    23401 tgcgatattg agggtaattc attcgtggaa tactcatagc tcttgcgtcc attcctttcc
    23461 cggcgtcata cgcaaaatgc cgtccacatt ctgcttgatg atgccgtatt cctgcatctg
    23521 ctttttcgct tcgccgtact tctcatacag ccgttccttt tcctcggcca atcgcttgta
    23581 ctcggctttc agggcgtaca gatcggggag ctttttcaca ccctgtccct ttagggcctt
    23641 tgccgcagct tcatacaaaa tcaacgtgct ttcatgctca cgccggaatt ttgccttatc
    23701 cgctgcattg cggtattcca tagccacggg ccagagctgc ttgtaggtag aaatattctt
    23761 tatcagcacc gccatatccg caagccggtg ttccatagct ttgagggtag cggcggcctc
    23821 gtcgctggcg gcggtgatct cggccacttt gctttccaaa tccgggtagg tgtctatccc
    23881 atgttcagtc aggaaattca aagtctttgc ggcctgcttc aaattgtgga gcttcgccca
    23941 cctctcatag cctgccgact gctgggcctt aatgttgttt tccagatcaa tccgcaaaga
    24001 aatccccttg cgctctttgg gcgctctggt gataacgcgg cctttgatac gctctttgat
    24061 ggcttcctcg gtgtaggctt cccccaatgt cttgcagcgg gtaaagcgtt cctgtccggg
    24121 agcgcggaaa gaaataaact ttcccggctt gatctcatag ccctgcgcct gcatgagccg
    24181 caggaaatca tcaaaatctt ttgcctgcgg gatagcggcg tcaatggctg ctttgagctt
    24241 cgctttccag ctcgtccctt tccgctgggc gtcccactcg gcatagctct tgcccttact
    24301 ctggccgggc ttcaccacgg acaatccgtt ttccttgcac agcctgtcgc tggtgcgccg
    24361 gatataggca tagctctgtt tgttggaaat gtacttgcgc tgatttacca tatccacggc
    24421 acagaaaata atgtgattgt ggacgtggcc cttatcaata tgggtagtca gcacataggg
    24481 atatttccct tgcagcactt cctcggcaag ctgcttcccg atctcatggg cctgttccgg
    24541 cgtggcttcg ccgggggcaa aggattgtat caggtggtgg cccagattat tgcccttttg
    24601 gtatgcctgc gccagcagca tttcaaattc aatgtccgct gtttcataag agcaggcaaa
    24661 ggaggccacc agcagcttgc cgtcagtttt gtccgggttc tcgatgtagt caagggcttt
    24721 tttcagcgtt gacttgatag gatgtatctt tgtaactgcc atatctgcgc cagcgccccc
    24781 tttatatcct cgatgtcctg tgcgtacacc ggccccatac cgttcagcct ctttgcaatc
    24841 tgattgacgt tgacgccgat cttctgtatc tcggcggtct gcgcccggat gtcgctggtg
    24901 tccatgttga cgatatagcc gtcaatgagc atcttccggg cgtaggctga aaagttcttc
    24961 gtatgcagca gcgccatttt ctgttcaatc atagcccgtt cctccggcgt cacagggacg
    25021 cgcagcacga tttttcgctt tctgtttgcc atgcttcacc gtcctttcca ttcaggggtt
    25081 tggggatttc cccaacaagc aaaacggacg cggcccggca gggacggggc aggccgtttt
    25141 tccgggagtg gctacactcc ctatgcttgc tacgttactg tttctccact aacaagacgt
    25201 ttccaaagcc ccaaactacc cgcaaaatag ccctctggaa agaaaaaagt gcaaaaaaat
    25261 agaagctacg ctgtgcagct cccgctaaaa gtaaaacaga ggatgccagt tacacatcct
    25321 ccgtttcatt cttcttcggc ttttcaatta gtatggcgct gtcaatcgcg ccctcgatga
    25381 tcggcaggta ttcttcgggg cagagtttca gcttgtggct gaccctttgc cgcagttcgc
    25441 tttcctcccg catgatctcc gggttaaagt agcgttccac aggaagcccg caggctttaa
    25501 taagctgtat gacaaccggc agactgggaa tggcccgtcg ttttcgatgt tggcaagata
    25561 gcgccagtca atatttattt tctctgccat tgctttgcgc gaaacgcgct ttgcttgccg
    25621 ggccgctttc acatccgcgc cgaaagtttc aaagccggga caatcttcaa tattcgccat
    25681 atcgcacctc ccacttacat tgtatatttc atactctgcc cgtggaatgt tggtatatgc
    25741 acctttctta aagtttatat ttcacatttt gtttctttta ttcttcgagc aaaagaatta
    25801 taatgtaccc tgtggaggtg tactatggac tacatgacgt tgaaagaggc aggcgagaaa
    25861 tggggtgtgc ccccacgatg gataaactac tattgtgctg cgggacgcat ccccggcgct
    25921 gtaaagatgg cgacgatctg gctcattccc aaaactgcgg aaaagccgat tgacaggcgt
    25981 acaaagaaag gaaaggcggt tactgaatag tataatgaaa ccggcgcatt ttggatatgg
    26041 ttttgaagag agagaaagca tggtattttg tcacggaaaa actataagat gatagatggc
    26101 aggctgctcc agaccgataa aaagtattct catctgaaac agaaagaaaa gatttacaca
    26161 tggatgtatg aagaagcgaa actttatcat gacagaaatg gaaaatgccc gaaacaaaga
    26221 cgaggaagct gctgctgtat cagccgtata tggtagaatt gaaaaggccg gcatctggat
    26281 tctttatggt gaagtctcca gacattggat aactgctggt ctaatgcgcc tttgagtatg
    26341 gggtgattga ttttaatacc ggggaggtac ggctttttga ggaaaatatt ctgggattta
    26401 catttggcga ttattacatt tcctgtaact gcaaggacac ggatgatgaa gatatgagga
    26461 atatagactt gctgattttc tactgtcttg aacggtatta ggactgaatc tgctgcgcgg
    26521 agtgcggcaa gagcgaccgg agggagtaca cacaggccct gcctgtgtga aaagccatac
    26581 cgcaaggtgc attcgcaaaa aagaggccgc gcattttgct gaggcttggg gtgtccccaa
    26641 caagcaaaac gagaacgccg caggcgggca agtggtggat cagaatatcc gtaaaggata
    26701 ttctgatcca ttgcttgcca gtattatcgg gatgttttga caaccgctgt aatcgaaaca
    26761 tcccaaacat taagaaggtg ttaattatta ttatcttgat ttaattcgtt taaaccaata
    26821 atatagatac atacaccatg aaaagaagcg agtatcccaa tgacggatga ttaaaatccg
    26881 aaaagaaatc gctttgaaac tacagggaaa ctacagactg ttatgttatc ttcttaaaat
    26941 ggagggattt ttatgtcgat acgaattcta cttgtcgagg atgatgatca tatctgtaat
    27001 acagtaaggg cgtttctggc tgaggcagga tatcaggtgg atgcctgcac agatggaaat
    27061 gaggcataca ccaagtttta cgaaaacact tatcaactgg ttattcttga tattatgctg
    27121 cccggtatga acgggcatga acttttgcgt gaatttcgtg cgaaaaatga tactcccatt
    27181 ctgatgatga cagccctgtc ggatgacgaa aaccaaatcc gggcgtttga tgcagaggca
    27241 gacgactatg taacaaagcc attcaagatg cagattttac taaagcgggt ggaagccctg
    27301 ctgcggcgca gcggtgcgct ggcaaaggaa atccgtgtcg gcaggctgac acttctgccg
    27361 gaggatttca cggtactttg tgacggtaca gagctgcccc tgacacgaaa agaatttgaa
    27421 atccttttgc tgctggtgca gaacaaaggc agaaccttaa cgcatgaaat cattttgtca
    27481 cgtatatggg gatatgactt tgagggtgat ggcagcacag tccacactca tatcaaaaat
    27541 ttgcgggcca agctgccgga aaatatcatc aaaaccattc gcggtgtagg ataccgattg
    27601 gaggaatcat aataatggaa agaaaaggga ttttcattaa ggttttttcc tatacgatca
    27661 ttgtcctgct actgcttgtc ggtgtaacag caacactgtt tgcacagcaa tttgtgtctt
    27721 atttcagagt gatggaatta cagcaaacag taaaatccta tcagccattg gtggaactga
    27781 ttcagaatag cgataggctt gatattcaag aggtggcagg gctgtttcac tacaataacc
    27841 aatcctttga gttttatatt gaagataaag agggaagcgt actctatgcc acaccaaatg
    27901 ccaatacatc aaatagtttt agacccaact ttctttatgt ggtacataga gatgataata
    27961 tttcgattgt tgctcaaagc aaggcaggtg tgggattgct ttatcaaggg ctgacaattc
    28021 ggggaattgt tatgattgcg ataatggttg tattcagcct tttatgcgcg tatatctttg
    28081 cgcggcaaat gacaacgccg atcaaagcct tagcggacag tgcgaataaa atggcaaacc
    28141 tgaaagatgt accgccgccg ctggagcgaa aggatgagct tggcgcactg gctcatgata
    28201 tgcattccat gtatgtcagg ctgaaagaaa ccatcgcaag gctggaggat gaaatcgcaa
    28261 gggaacatga gttggaggaa acacagcgat atttctttgc ggcagcttct catgagctaa
    28321 aaacgcccat cgcggctaca agcgttctgt tggagggaat gcttgaaaat atcggtgact
    28381 acaaagatca ttctaagtat ctgcgcgaat gcatcaaaat gatggatagg cagggcaaaa
    28441 tcatttccga aatactggag cttgtcagcc tgaatgatgg gagaatcgta cccatagctg
    28501 aaccgttgga catagggcgc acggttgccg agttgctgcc cgattttcaa accttggcag
    28561 aggcaaacaa ccagcggttc gtcacagata ttccagccgg gcaaattgtc ctgtccgatc
    28621 cgaggctgct ccaaaaggca ctatccaatg tcatattgaa tgcagttcag aacacgccgc
    28681 agggaggcga ggtacggata tggagtgagc ctggtgctga aaaatgccgc ctttttgttt
    28741 tgaacatggg cgttcacatt gatgatactg cgcttccaag gctgttcacc ccattctatc
    28801 gcattgatca ggcgcgaagc agaaaaagtg ggcgaagcag aaaaagtggg cgaagcggtt
    28861 taggacttgc catcgtacaa aaaacgctgg atgccatgag cctccagtat gcgctggaaa
    28921 acacctcgga tggcgttttg ttctggctgg atttaccgct cacatcaaca ttgtaaatat
    28981 ttaaaactta aatgattttg ggctctttgt caaataggac tgatagagtg agataagaac
    29041 atctaaggta gaattccttt tctactttag atgttttttg attcataggt aatcagatta
    29101 tttagtaaga aaattctggt cttcattttc cgaaagcttc gaaagccata agcgactcgt
    29161 ttcaatgctt tgatgtgggt atgcagattt tctattttgg catttgaata aggcaattcg
    29221 agggctaaac gaatgccgga ttcatagttt ataaaagctt tttttataga atgcagttct
    29281 tttggaacgg aattgggcat cgttcttaca agtttaaaga aagtgtccgc ctctttgtcg
    29341 tgaaacgctt gaagaatttc ttgatagtaa aaataggcga ttcgtaattc gtcagagatc
    29401 gaaagcagac gatccaccac ttcactttcg gtgagatacg cccaaccaaa attgcgccat
    29461 ttggtccatt tgtcataaac aagcttgtcc tgtcgtttga tcagaagccg agacaacgcc
    29521 tttaattgtc gataatattt ggcttgtgtt ggatcgtttc gatcaaagct tttcatgatt
    29581 cgtacgcgaa tatcctcaaa tttacgaatg agatgtttga caatgtgaaa acgatccaca
    29641 acgagttttg cctttggaaa acattccttt atcagagagg cataagaaac attcatgtcc
    29701 gtcacgatga cctttacctt caatcgtgcg cgtcgttcaa attgcataaa ataagaaacg
    29761 agagcctttt tcttccgtga aggaagaata tccagtaatt ttccagtatc gccatccata
    29821 caagaaaagg agtagggacc gacttgattt ttcagtgaac gaaattcatc cattaataaa
    29881 caacgcggta accaatttct ttttactttt gtcatgggga tcagagaagc gagtgtccga
    29941 gaaacggtcg accaagaaac gccgcaatgc ttagcaattg tcgtcattgc ttgtttttca
    30001 gttagctctt ctaaaatttt atagcgcact tgtttagcga taaagcagtt tctatcgaca
    30061 atgtaggtat tcgccgtaaa ataggtgtgg cagccacgac agtggtagcg ttgtttcgct
    30121 aaagcaagat aagtcagttg gttcgcacat ttattaagtt gaatgagcga ttttttcttt
    30181 ccgttcttaa cgatcatgcc ttcatgattc atctgtttac acaaaggaca gcgacgaatt
    30241 tctttactta acgtcgcaga aacaacaaga ctcttgatat tcttaatgct cttcgattcc
    30301 acatttgtga tgaaaatgtt tttatctttg attccaagta gtagtttgat atgatgaaga
    30361 tcaggcatac agaacgctcc tttgattgtg ttgtggtgat tcaattctaa cggagcttct
    30421 gtatgttttg ttttttcatg ctcaaaaggg accgatagaa tgattttcta tcagtcctag
    30481 aaattataga gccgattttg accgacaggt ataaccctgc cggtcttttt ttgctgctac
    30541 aggaaaacta cagactggct acagggaaag tacagatgcg cttgccataa taacaatcgt
    30601 accagccaca aatcgtagtt ttattgcaaa ggaggcattc gatcaaatgg aaaaaagcaa
    30661 ctatcattcc aatgcggatc atcacaaacg gcatatgaaa caatccgtgg aaaagcgggc
    30721 ttttctatgt gcgttcatta tttcgttcac agtctgcacg ctgtttttgg ggtggagact
    30781 ggcttccgta ttggaggcaa cacagatacc gcccatccct gcaactcata caggcagcag
    30841 cactgacgta gtggagaatt tggaggaaaa cgctcttgcc accgccaaag aacagggaga
    30901 tgaacaggaa tggagcctga ttttagtgaa caggcagaac cccatccccg cacagtacga
    30961 tgtggagctt gagcaactat caaatggtga gcggatagat attcggattt ctccctatct
    31021 tcaagatttg tttgatgccg caagaactga tggagtttac ccgattgtcg catccggata
    31081 ccgaacaaca gaaaaacagc aagaaattat ggatgaaaaa attgccgaat ataaggcgaa
    31141 aggctacacc tctgcacagg ctaaagcgga agcagaaact tgggtggccg tgccgggaac
    31201 gagcgagcat cagcttggtc ttgctgtgga tatcaatgcg gacggaattc attcaacagg
    31261 caacgaggtt tatagatggc tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta
    31321 cccaccagac aagacagaga taaccggtgt gagcaacgag ccgtggcatt accgatatgt
    31381 tggcatcgaa gctgccacag agatgtacaa ccaaggggtt tgccttgagg aatatttaaa
    31441 accagaaaaa tgagaaaagg atgttatgtt atggacagaa aaagattgac acagcgtttc
    31501 ccgttcctgc ttccaatgag acgggcgcag agaaaaatgt gcttttatgc gggaatgaga
    31561 tttgacggct gtcgctatgc gcagacgata ggggaaaaat cgctttccca tttgctcttt
    31621 gaaacggatt gcgcattata caaccacaat accggatttg acatgatata ccaggaaaac
    31681 aaggtgttca acttaaagct ggcagcaaag accttaaacg gcttattgat aagaccgggg
    31741 gaaacctttt ctttctggtt gttcctgcat acgccattga caattatcca gcgcagaggt
    31801 cacgaagtaa aggagtttcc agagccaaac agtgacgaga ttaaaggggt ggatgcaacc
    31861 atttcagagg gctggattga tttaaaagtg cgaaacgata ccgactgcac ctaccaaata
    31921 tgggtgacct tagatgatga gaaaatcatc ggtcaggtgt tcgccgacaa agagccccaa
    31981 gcattataca aaattacaaa tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat
    32041 gaatatgccc aggttaaacg gatgcaagtt gccttaggta ccggggaaat aatagattgc
    32101 aagctgcttt atacaaacaa atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
    32161 caggaggaga accaatgaga aaaagtgtgg gcattactgt ttttggatgc gaacaggatg
    32221 aggcaaatgc tttccgcgct ttatcgccgg attttcatat tatccctacg ctgattagcg
    32281 atgcgatatc ggcagacaac gcaaaattgg ccgctggcaa tcaatgcgtt agcgtaagcc
    32341 ataagtccga ggtttccgag gcgacaattc ttgcgctgag aaaggtcggg gtaaaataca
    32401 tttctacccg cagcatcggc tgcgatcaca ttgatacgac tgccgccgag agaatgggaa
    32461 tctcggttgg cacggttgcg tattcgccgg acagcgttgc ggattatgct ttgatgctga
    32521 tgctgatggc catacggggt gcaaaaccca ccatgcacgc cgtggcgcaa caagatttca
    32581 gattggatcg tatccggggg aaagaactgg gggatatgac tgtgggagtt attggaaccg
    32641 gccatatcgg gcaagcggtc gtcaaaaggc tgcggggatt tggatgccat gtgctggcct
    32701 atgataacag ccgaaaaatg gatgcagatt atgtccagct tgatgagctt ctaaaaaaca
    32761 gcgatattgt tacgctccat gtgccgcttt gtgcggatac ccgccatctg atcggtcaga
    32821 agcaaattgg agagatgaag caaggcgcat ttttaatcaa caccgggcgc ggagcgcttg
    32881 tcgataccgg ggcgctggtg gaggcgttgg aaagcggaaa gctgggtggt gcggcactgg
    32941 atgtgttgga gggcgaggat cagtttgttt ataccgattg ctcgcagaaa gtgcttgacc
    33001 atcccttttt gtcgcagctt ttaaggatgc cgaatgtgat catcacaccc catacggcgt
    33061 actacactga gcgtgtgctg caggatacta cagaaaaaac aatcaggaat tgtctcaatt
    33121 ttgaaaggag tttacagcat gaatagaata aaagtcgcaa tcatcttcgg cggttgctcg
    33181 gaggaacatg atgtgtcggt aaaagccgca atagaaattg ctgcgaacat tgatacggaa
    33241 aaattcgatc cgcactacat cggaattaca aaaaacggtg tatggaagct atgcaagaag
    33301 ccatgtacgg aatgggaagc cgacagtctc cccgccatac tctccccgga taggaaaacg
    33361 catgggctgc ttgtcatgaa agaaagcgaa tacgaaacac ggcgtattga tgtggctttc
    33421 ccggttttgc atggcaaatg cggggaggat ggtgcgatac aggggctgtt tgtattgtct
    33481 ggtatcccct atgtgggctg tgatattcaa agctccgcag cttgcatgga caaatcactg
    33541 gcctacattc ttacaaaaaa tgcgggcatc gccgttcccg aatttcaaat gattgataaa
    33601 ggtgacaagc cggaggcggg tgcgcttacc taccctgtct ttgtgaagcc ggcacggtca
    33661 ggttcgtcct ttggcgtaac caaagtaaac ggtacggaag aacttaacgc tgcgatagaa
    33721 gcggcaggac aatatgatgg aaaaatctta attgagcaag cgatttcggg ctgtgaggtc
    33781 gggtgtgcgg tcatggggaa cgaggatgat ttgattgtcg gcgaagtgga tcaaatccgg
    33841 ctgagccacg gtatcttccg catccatcag gaaaacgagc cggaaaaagg ctcagaaaat
    33901 gcgatgatta cagttcccgc agacattccg gtcgaggaac gaaatcgggt gcaggaaacg
    33961 gcaaagaaag tatatcgggt gcttggatgc agagggcttg cccgtgttga tctttttttg
    34021 caggaggatg gcggcatcgt tctaaatgag gtcaataccc tgcccggttt tacatcgtac
    34081 agccgctacc cacgtatgat ggccgccgca ggaatcacgc ttcctgcact gattgacagc
    34141 ctgattacat tggcgttaaa gaggtgataa gtatggaaaa tggttttttg tttttagatg
    34201 aaatgttgca tggcgtccgt tgggatgcca agtacgccac atgggataac ttcacaggaa
    34261 aaccggtgga tgggtatgag gtgaatcgca tcatcggcac aaaggccgtg gcgtttgctc
    34321 tgcgcgaggc acaaatccat gcggctgcgc ttggctatgg cttgctttta tgggatggat
    34381 atcggccaag aactgcggtg gactgcttcc tgcgttgggc agcgcaaccg gaggacaatc
    34441 tcacaaaaga aaaattttac cccaatatag agcgagccga gttgattaca aagggttatg
    34501 tggcctcaca atccagccat agccgtggaa gcgcaattga tcttacgctc taccacctgg
    34561 atacagggga acttgtttca atgggaagta acttcgattt tatggacgaa cggtcgcatc
    34621 atacagcaaa agggataggg gatgcagagg cacaaaatcg aagatgcttg cgtaaaatca
    34681 tggaaagcag cggatttcag tcttatcgct ttgaatggtg gcactataag ttgattgatg
    34741 agccataccc cgatacctat tttaattttg ccgtttcata atgaaagtat tggttttgta
    34801 tcagattttg gatttgataa aatctatgct ggtcatggac aatcttttac tggaaagtgg
    34861 ttaaagcatc ttttgtaata cattgcaaat tatagtattc aatctgccca gcggcagaaa
    34921 agaaaaaaac cgctgttggg cagacctttc ccacgcccat tttgtatttt gctggcggcg
    34981 gttttgagaa atcaaggccg ccgttctttt ctggcagcaa aaggtgtggc ggcccatagg
    35041 aggatgccgc tatgctgttt tgctattgcc atgctctaat ccacttccac cactcaaaaa
    35101 aagccttgcc cctc
//
LOCUS       DAYYGS010000020        34959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000020 DAYYGS010000000
VERSION     DAYYGS010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.020"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="ACHA44_000928"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="ACHA44_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HFM8904238.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="ACHA44_000929"
     CDS             complement(2020..2922)
                     /locus_tag="ACHA44_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8904239.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="ACHA44_000930"
     CDS             complement(2919..3611)
                     /locus_tag="ACHA44_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HFM8904240.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="ACHA44_000931"
     CDS             complement(3654..4295)
                     /locus_tag="ACHA44_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HFM8904241.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="ACHA44_000932"
     CDS             complement(4333..4647)
                     /locus_tag="ACHA44_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904242.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA44_000933"
     CDS             complement(4635..5264)
                     /gene="pyrE"
                     /locus_tag="ACHA44_000933"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFM8904243.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA44_000934"
     CDS             complement(5271..5987)
                     /gene="pyrF"
                     /locus_tag="ACHA44_000934"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFM8904244.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5984..6916)
                     /locus_tag="ACHA44_000935"
     CDS             complement(5984..6916)
                     /locus_tag="ACHA44_000935"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HFM8904245.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="ACHA44_000936"
     CDS             complement(6913..7689)
                     /locus_tag="ACHA44_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HFM8904246.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA44_000937"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="ACHA44_000937"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFM8904247.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /locus_tag="ACHA44_000938"
     CDS             complement(10933..12009)
                     /locus_tag="ACHA44_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HFM8904248.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="ACHA44_000939"
     CDS             complement(12014..13294)
                     /locus_tag="ACHA44_000939"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFM8904249.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="ACHA44_000940"
     CDS             complement(13313..14239)
                     /locus_tag="ACHA44_000940"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HFM8904250.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="ACHA44_000941"
     CDS             complement(14252..15535)
                     /locus_tag="ACHA44_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HFM8904251.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA44_000942"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="ACHA44_000942"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HFM8904252.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="ACHA44_000943"
     CDS             complement(16323..17234)
                     /locus_tag="ACHA44_000943"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8904253.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA44_000944"
     CDS             complement(17231..17695)
                     /gene="lspA"
                     /locus_tag="ACHA44_000944"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFM8904254.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="ACHA44_000945"
     CDS             complement(17700..18398)
                     /locus_tag="ACHA44_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HFM8904255.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="ACHA44_000946"
     CDS             complement(18385..18771)
                     /locus_tag="ACHA44_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904256.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="ACHA44_000947"
     CDS             complement(18918..20588)
                     /locus_tag="ACHA44_000947"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFM8904257.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="ACHA44_000948"
     CDS             20771..21004
                     /locus_tag="ACHA44_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8904258.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="ACHA44_000949"
     CDS             complement(21103..21534)
                     /locus_tag="ACHA44_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HFM8904259.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="ACHA44_000950"
     CDS             21613..22026
                     /locus_tag="ACHA44_000950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HFM8904260.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="ACHA44_000951"
     CDS             complement(22064..23212)
                     /locus_tag="ACHA44_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFM8904261.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="ACHA44_000952"
     CDS             complement(23334..23930)
                     /locus_tag="ACHA44_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8904262.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA44_000953"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="ACHA44_000953"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFM8904263.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="ACHA44_000954"
     CDS             27160..27591
                     /locus_tag="ACHA44_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904264.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="ACHA44_000955"
     CDS             complement(27636..28985)
                     /locus_tag="ACHA44_000955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8904265.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="ACHA44_000956"
     CDS             complement(29055..30218)
                     /locus_tag="ACHA44_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8904266.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="ACHA44_000957"
     CDS             30347..30670
                     /locus_tag="ACHA44_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904267.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="ACHA44_000958"
     CDS             complement(30853..32202)
                     /locus_tag="ACHA44_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8904268.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="ACHA44_000959"
     CDS             complement(32271..33224)
                     /locus_tag="ACHA44_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFM8904269.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="ACHA44_000960"
     CDS             complement(33326..34516)
                     /locus_tag="ACHA44_000960"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFM8904270.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(34716..>34959)
                     /locus_tag="ACHA44_000961"
     CDS             complement(34716..>34959)
                     /locus_tag="ACHA44_000961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904271.1"
                     /translation="RKNRTVTRLLSQSFFRIQQVKLLRNIQRVKLKIADKRRWKFYVF
                     LLVYAINKLNIQNTIDFNERASINGCVIFICPGRVK"
BASE COUNT         9950 a         7641 c         6120 g        11248 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaact atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatataacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccga
//
LOCUS       DAYYGS010000021        34662 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000021 DAYYGS010000000
VERSION     DAYYGS010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.021"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            591..1280
                     /locus_tag="ACHA44_000962"
     CDS             591..1280
                     /locus_tag="ACHA44_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8904272.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="ACHA44_000963"
     CDS             1267..2475
                     /locus_tag="ACHA44_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8904273.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="ACHA44_000964"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="ACHA44_000964"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFM8904274.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA44_000965"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="ACHA44_000965"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFM8904275.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="ACHA44_000966"
     CDS             complement(6578..7273)
                     /locus_tag="ACHA44_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HFM8904276.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA44_000967"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="ACHA44_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFM8904277.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="ACHA44_000968"
     CDS             complement(8472..8663)
                     /locus_tag="ACHA44_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HFM8904278.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="ACHA44_000969"
     CDS             complement(8684..9574)
                     /locus_tag="ACHA44_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFM8904279.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="ACHA44_000970"
     CDS             complement(9561..10328)
                     /locus_tag="ACHA44_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8904280.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA44_000971"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="ACHA44_000971"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFM8904281.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA44_000972"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="ACHA44_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFM8904282.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYLTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA44_000973"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="ACHA44_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFM8904283.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="ACHA44_000974"
     CDS             14899..15282
                     /locus_tag="ACHA44_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904284.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA44_000975"
     CDS             complement(15450..16208)
                     /gene="jag"
                     /locus_tag="ACHA44_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HFM8904285.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="ACHA44_000976"
     CDS             complement(16226..17044)
                     /locus_tag="ACHA44_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HFM8904286.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA44_000977"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="ACHA44_000977"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFM8904287.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA44_000978"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="ACHA44_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFM8904288.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA44_000979"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="ACHA44_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFM8904289.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA44_000980"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="ACHA44_000980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFM8904290.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA44_000981"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="ACHA44_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HFM8904291.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="ACHA44_000982"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="ACHA44_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFM8904292.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA44_000983"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="ACHA44_000983"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFM8904293.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA44_000984"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="ACHA44_000984"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFM8904294.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDIAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKENQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA44_000985"
     CDS             27086..27385
                     /gene="rpsF"
                     /locus_tag="ACHA44_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFM8904295.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA44_000986"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="ACHA44_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8904296.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA44_000987"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="ACHA44_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFM8904297.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="ACHA44_000988"
     CDS             28352..30325
                     /locus_tag="ACHA44_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HFM8904298.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA44_000989"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="ACHA44_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFM8904299.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA44_000990"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="ACHA44_000990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFM8904300.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="ACHA44_000991"
     CDS             33204..34496
                     /locus_tag="ACHA44_000991"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFM8904301.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10566 a         6734 c         6853 g        10509 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tgtattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttaaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagat caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgtaaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggatcaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggtggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccggc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagacatt gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atggggaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatcc
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaatcct tgaaaactat
    25441 atcgaacatc aacgcgaagt catcacacga cggacggtct ttgaaaaaga aaaagcagaa
    25501 gcaagagcac acatcttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaggcaaaag caacaatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgtacagc caaaggaatc ccaatcatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggtcagagg gatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aaaatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaataa ccatcaagac taagtgtgta agctagcaag
    26881 aataaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataattag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattagatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaat gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atattgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaagagg tagtgaaaga gacgtttagc cttattgccg ataggctgtc
    34621 tcttgaacac tgtttattca gaataaggga ctgtgacaaa aa
//
LOCUS       DAYYGS010000022        33796 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000022 DAYYGS010000000
VERSION     DAYYGS010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.022"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(27..1259)
                     /locus_tag="ACHA44_000992"
     CDS             complement(27..1259)
                     /locus_tag="ACHA44_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFM8904302.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1514..2083
                     /locus_tag="ACHA44_000993"
     CDS             1514..2083
                     /locus_tag="ACHA44_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904303.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2261..2701)
                     /locus_tag="ACHA44_000994"
     CDS             complement(2261..2701)
                     /locus_tag="ACHA44_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFM8904304.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2859..3623
                     /gene="map"
                     /locus_tag="ACHA44_000995"
     CDS             2859..3623
                     /gene="map"
                     /locus_tag="ACHA44_000995"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFM8904305.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3655..4578
                     /locus_tag="ACHA44_000996"
     CDS             3655..4578
                     /locus_tag="ACHA44_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HFM8904306.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4803..5042)
                     /locus_tag="ACHA44_000997"
     CDS             complement(4803..5042)
                     /locus_tag="ACHA44_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904307.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5047..5466)
                     /locus_tag="ACHA44_000998"
     CDS             complement(5047..5466)
                     /locus_tag="ACHA44_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8904308.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHA44_000999"
                     /gene_synonym="mefH"
     CDS             complement(5693..6931)
                     /gene="mef(H)"
                     /locus_tag="ACHA44_000999"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8904309.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7038..7673)
                     /locus_tag="ACHA44_001000"
     CDS             complement(7038..7673)
                     /locus_tag="ACHA44_001000"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904310.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(7787..7879)
                     /locus_tag="ACHA44_001001"
                     /pseudo
     CDS             complement(7787..7879)
                     /locus_tag="ACHA44_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(8015..8650)
                     /locus_tag="ACHA44_001002"
     CDS             complement(8015..8650)
                     /locus_tag="ACHA44_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8904311.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9162..10304
                     /locus_tag="ACHA44_001003"
     CDS             9162..10304
                     /locus_tag="ACHA44_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8904312.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10297..10503
                     /locus_tag="ACHA44_001004"
     CDS             10297..10503
                     /locus_tag="ACHA44_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8904313.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10582..11775
                     /locus_tag="ACHA44_001005"
     CDS             10582..11775
                     /locus_tag="ACHA44_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8904314.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11980..13119
                     /locus_tag="ACHA44_001006"
     CDS             11980..13119
                     /locus_tag="ACHA44_001006"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HFM8904315.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13112..13912
                     /locus_tag="ACHA44_001007"
     CDS             13112..13912
                     /locus_tag="ACHA44_001007"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8904316.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13912..14739
                     /locus_tag="ACHA44_001008"
     CDS             13912..14739
                     /locus_tag="ACHA44_001008"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8904317.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14744..15451
                     /locus_tag="ACHA44_001009"
     CDS             14744..15451
                     /locus_tag="ACHA44_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904318.1"
                     /translation="MRSVCIATFNGGRYLREQLDSILSQLSAEDEVVISDDGSVDDTW
                     DILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWLETKVKKITD
                     YFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYIGAGMAFRSS
                     LKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIETHSKLRQKIS
                     WRVHLITALIKRKWFKR"
     gene            15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHA44_001010"
     CDS             15551..16417
                     /gene="rfbA"
                     /locus_tag="ACHA44_001010"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFM8904319.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHA44_001011"
     CDS             16431..17003
                     /gene="rfbC"
                     /locus_tag="ACHA44_001011"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFM8904320.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHA44_001012"
     CDS             17025..18053
                     /gene="rfbB"
                     /locus_tag="ACHA44_001012"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFM8904321.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHA44_001013"
     CDS             18151..19002
                     /gene="rfbD"
                     /locus_tag="ACHA44_001013"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFM8904322.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19036..21069
                     /locus_tag="ACHA44_001014"
     CDS             19036..21069
                     /locus_tag="ACHA44_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904323.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21113..22393
                     /locus_tag="ACHA44_001015"
     CDS             21113..22393
                     /locus_tag="ACHA44_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HFM8904324.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22603..23409
                     /locus_tag="ACHA44_001016"
     CDS             22603..23409
                     /locus_tag="ACHA44_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904325.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23421..24641
                     /locus_tag="ACHA44_001017"
     CDS             23421..24641
                     /locus_tag="ACHA44_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904326.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24631..26217
                     /locus_tag="ACHA44_001018"
     CDS             24631..26217
                     /locus_tag="ACHA44_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8904327.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26256..28394
                     /locus_tag="ACHA44_001019"
     CDS             26256..28394
                     /locus_tag="ACHA44_001019"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904328.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28762..29745)
                     /locus_tag="ACHA44_001020"
     CDS             complement(28762..29745)
                     /locus_tag="ACHA44_001020"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HFM8904329.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30123..31514
                     /locus_tag="ACHA44_001021"
     CDS             30123..31514
                     /locus_tag="ACHA44_001021"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8904330.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31530..32570
                     /locus_tag="ACHA44_001022"
     CDS             31530..32570
                     /locus_tag="ACHA44_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904331.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32589..33674
                     /locus_tag="ACHA44_001023"
     CDS             32589..33674
                     /locus_tag="ACHA44_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HFM8904332.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT        10936 a         5808 c         6671 g        10381 t
ORIGIN      
        1 ttttttgcat gtgtgcgaaa gtatactcaa gcccaatcgc catttcggaa aatcggtact
       61 gtagagccat cttcacggat tccgtcgata tccatcttgt cagatcctac catgaaatct
      121 acgtgggttt gacttctatt caatccagct tctgctaatt cttcatcgga catttctgta
      181 ccgcctttca cactgaaggc ataagcagaa cctagtgcta agtgattcga tgcgttttca
      241 tcaaataatg tattgaaaaa gatgatgcca gattgagaaa taggtgaagg gtcaggtaca
      301 agtgcgactt ctcctaaacg acgcgcacct tcatctgtat caagcagttt tgctaagaca
      361 tcttctcctt gttcggcaga aaaatcaaca acttttccat ctttaaaagt aaatttcata
      421 cccgagatga tggtgccagc ataactcagt ggctttgtac ttgaaatgta accatctact
      481 cgatgactgt caggtgctgt gaacacttct tccgtcggca tatttgccat gaatttttct
      541 ccacgagcat tatagcttcc agcgccttcc caaagatggt ttttaggcaa tccgataatg
      601 atatctgtac cgggagcagt atagtgaagg gcagaaaatt gttctcgatt caattcttct
      661 gctttttttg ctaatttttc atcatgcttc ttccatgcta agacagggtc ttcttcatag
      721 acacgagtcg ttttgaaaat ctgatcccat aatgcctcaa cttgtttttc ttctggcagt
      781 tctgggaaga cttttgctgc ccattgttta ccagccgcag ccacgacagt ccaactgact
      841 ttatttgctt gagttgcctt gcgtaaattc attagtgctt tgccattcgc tgattgataa
      901 gacgctactc gatggctatc tacaccagca aatgcatcgg gatcggcaga aacaacgctg
      961 atcctgcttg ctcctttttc aagccattca tccgcttgat cgattttaga ttgtggtaca
     1021 ttctcaatac gatccgttgc agcatgtaaa agaaattctc tttggatctg gtcgtcagtc
     1081 cattgaacga taacctcagc ggctcctagt ttataagctt cttgtgtgat caatcgagca
     1141 aggggtgcct gttccacgct gatctgtaaa acaactgtgt gtcctttttc tgtggctacg
     1201 ccagtttctg caattaagcg tgcatatttt tttagaagtt cattaaaatc agataacata
     1261 ataaaaattc ctcctcacca ttttttctgg taactattag catgatacca tttaattatg
     1321 attcaaacaa tgaggcttta aataaaaaaa ggaggttatt tttttaaggt taagtttcat
     1381 gatatatgtt actacttgct atttcagctt gtgaagaaaa tcaaaaacta attttgcata
     1441 tcaaaaaaaa catgttacaa tgaaattaat caatggtgga agcgttataa aaaaattaag
     1501 gagattaaaa aatatggcaa gaaaaaagac gattactaga gaacagattt tggcagctgc
     1561 ttatgaagta gctgccaccg atggatttac acgatttact gcgagaaaca tcgcgaacaa
     1621 aatgaattgt tccacgcaac cgatttatct tgagtttaaa aatatggatg acttgagaca
     1681 agctcttttt gagcagatct atgatcattt acaatatgaa gtgttcactg ttgagcatac
     1741 agggaatacg attatcgatc ttgcttttaa ttatatccat tttgcaagaa gagagaagaa
     1801 gttgtatcgt tcactgtact tagaagaata tggtggagga aaagagatgc agaatttctc
     1861 ttatacttac tttgtgaata cagtgaaacg cgatccagct tatgtgaatc tttcagaaga
     1921 gcagatcgaa tctttgtata atgggacatg gatcatagct actgggatcg cagctttgat
     1981 gtcttcaagc atcatccatc caactgatga acagattgaa aagatgatcc aagacagtat
     2041 agatgctatc ttgaaattca aagaaccaat cgaaatcgat tgaatatact caaaagttcc
     2101 acaaatcagt aggtaacaag agatgtgtca aaataaggaa aagttcgttt ttcttacagc
     2161 gtatgcattt ttaactcccg aagaactctt ccatgaatgc aatgaataaa agagccatac
     2221 agattgaata aatctggatg gctcttttat tgcaagtata ttaaattttt gcgactagtt
     2281 ctttagcgaa agcttctaaa ttttcgatat cttcttcttc agcattcaga tccactttaa
     2341 cattttcggc tccttttgat gcgcctgttt ttgcaaaagc agcatcgaaa tcatccaccg
     2401 atttgcagaa ttcatcgtaa aaagtatcac cagatccgca gacaccgtaa atttttccca
     2461 atagatccaa ttcttgcaag tcttcataaa agtctacgat ctcatcaggc agatcgccat
     2521 catcgtatgt ataagtagct actacgcaaa tatctgcctc ctcgaattca tctgcatcta
     2581 cttgggtaca ttcgttgatt tcgacttcga tatccagatt ttcaaatgct tctgcaacaa
     2641 tatcggcgat ttcttcagtg tttcctgtca tgctagcata tacgattttt gctaaagcca
     2701 tgatgtgcct cctcgtttat aattatttaa caattatgaa ttataccaaa acatcattga
     2761 aaagaaaata aaacctagag acacctgggg aatgtcattt ggctaaatgt atgccgctat
     2821 gttaaaatga gagggatttg taaaaaggag acgaaaatat gattacttta aaatcagcac
     2881 gtgaaattga aatgatggca gaatctggag ctttattagc agatgtacat aaaaatctgc
     2941 gtgattttat aaaaccagga atcacaagtt gggatattga ggtatttgtc agaaactata
     3001 tcgagagtca tggcggaatt gctgcgcaaa ttggctttga agggtatgaa tatgcgactt
     3061 gtatcagtat aaatgatgaa atttgtcatg gattccctcg taaaaagcct ttgaaaaacg
     3121 gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg cggcatttct gattcttgtt
     3181 gggcctatgt agttggagaa tctacacctg agatcgaccg attgatggaa gtaacgaaga
     3241 aagctttgta catcggtatt gagcaagcac aagtagggaa tcggattggt gacatcggtc
     3301 atgctatcca aacttatgta gaaggcgaaa atctagcgat cgttcgtgat tttattggtc
     3361 atggcgttgg tcctacgatt catgaagaac cagcagttcc tcattacgga gaagctggaa
     3421 aagggttgcg gttgaaagaa ggcatggtta tcacgatcga acctatggtt aataccggaa
     3481 cttggaaaat gaaaatggat cctaacggct ggactgcgta tacaagagac ggcggacttt
     3541 cttgccaata tgaacataca ttggctatta ctaaagatgg tccaagaatt ttgacatcac
     3601 aaggagaaga aggcacttat tgatctatcc agtcaatagg tacgggagag gtacatgaaa
     3661 gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct ttattgagac gactcaaaag
     3721 cgcatcactg attcagaaat gagtacgacc tctgtcgtcg ttgcttatta tttactcttg
     3781 tctttatttc cattgattat tgcttttggt aatattttac cgtttctcca catcgatcaa
     3841 gagactgtac tgacctattt acgagaagtg attcctgaga ccgtttacca gtttatcggt
     3901 cctgcaatca aagacctttt gacacaaagt tcaggaggct tgttatccgt ttctgcatta
     3961 gcggctttat ggtctgcaag tcaaagtatc aatgctttgc aaattgcgat gaataaagcg
     4021 tatggcgtag aaaatagaaa aaattttttg atcgtccgtt tcttctcact aattgtcatt
     4081 gtcttgttca tgattgctat tagtggggta actatcgttc ttgggttggg acagctgatt
     4141 ttggatgcta ttcagccaat cgtacaaata cccgtgaact ttatcgatca gttccaaact
     4201 ttgaagtggc caatcacatt ggtagcttta tttgtcatca tgttcttgat ttatttaatt
     4261 gtccctaatg ctcagttgaa gttgaagacg atcattccgg gcacgatctt cgctactgtc
     4321 ggctggatgc tgttatcaca agtgtttagt atctatgcta ggtattttgc aacgaggatc
     4381 agcggttatc agatcatagg aagttttatt gtactcatgc tttggcttaa ttttgctgct
     4441 acgatcatca ttttgggcgg aatcatcaat gcagttgttc aagaatacgt gacgggtcat
     4501 caaatcaaaa aacgtcgaga tccaagccga aaatggttca ataaattgaa aactaaattt
     4561 tctcataaaa aaaagtagaa acaagagttc ttattgtaaa tttatatcag taatttattt
     4621 gaaattttat atctaatttt caattatccg cataaacact atacattcaa gcattttgaa
     4681 gtatagaaaa acactcatct taccacgaat ttaccacgat tgaatgagcc ttgatatgac
     4741 cgtaaaacta ctaagggaga tagcacgaaa ctgtgtatct cccgattttt ttatacagtt
     4801 gattacttca ctccgatttc aaatttcaat attgctttca tcatgtctgt atgaatttct
     4861 ccttttagtt cttcatcaac ctgcataatg attttaccac tttcagagtg gtaaaaagga
     4921 cgcaagcata atttagaaat ataaccgtca taaaacttca aaatctcttt aactgccatt
     4981 tcatctccgc ttgacgcttt gcaaatcaat tcaaaagagg gatagccata atctttactc
     5041 atttctttac ttcctttcgc tcaaaagcat ttttaacttc cttagtcctc tagttcttct
     5101 actgcaaata gcagaacgtg atacatggta cagttctgaa atttctctgt cgttcattcc
     5161 ttgaaaataa cgtaataaaa tcgcactacg ctgtttttct gacagtctat gtaaaactgt
     5221 accaagtgtt tcatcagata cataaattgt aaagtgcaag actttaaatg atacacaatc
     5281 ataaaaacag gtatcaatcg ttccacgttc aacttgtttc gtttcggcta tttcacaaaa
     5341 caatacttca cgtttagaat gtcttgcaag ctgtctgttt cgacttttca ctttgttctt
     5401 aatgacaatc agcattaaag aattgaactg taatctgacg atatgctcga aagaagatgt
     5461 cctcataatc tcaccccctt tctgctaatg ctgaaatata taaacggaat accgtttata
     5521 ccctttagaa atacagaaaa atggtaagaa aaaagcccac acaaaacata tatttgtgcg
     5581 agccttaaat actgcaatat tactctttct tttataacac tatcctttta gcagatttca
     5641 aattttcttt tatatattgt ttttagccta acatactatt taaccccttg tattatatct
     5701 ctaacttttg aaaaattctt tttgaatata ttgaaatcat gattgaagca atagaagcta
     5761 caaacataac aacccatgtt tcttgtataa atacatcgaa taaaataccg taaaccattt
     5821 gtccaatagg ttgaccgcac atagcaactg ctaccagcgt agccattatc tttcctacta
     5881 attgcggtgg tgtttgtgtc tgaacaagag tgtaaatttg aacaacaaac atagtagata
     5941 cacccatagc cacaaagcac atcactgtaa ctacccagta ggaaactatt gcaggcaatt
     6001 ctaaaaatag cgaaattccc ataagtaaaa cagaaacaga acaaataagt aacaaggtat
     6061 aaaggttttt tagttttaac tttgaagcaa caagagctgt taaaatacca ccacataaac
     6121 cacctaatga taatgctgat tgtgaaaaac catatagtgt atctggcatt tttaaaactt
     6181 cagaaattaa gataggcaga ccaacaatta aaacagcact taaaaccaaa ttgaaaatgc
     6241 aaaccaaaaa gattacggat ataaaaatgg gtttctcctt tttcaaaaag gtataactat
     6301 cttttaaatc gtttttagca actgcaatta cacctacttc acgaggtctt ttttggtgtg
     6361 gaatatggat aaaaatttcc ataatggctg aaaaagtaaa acatataata ctaacaatta
     6421 aaatgggatt gataccataa aattgaaaaa ctactccgct tatagctggt ccgataaagt
     6481 ttgctaaagt atttacttga tttattatag cattaccgac ggtaagttta tctgatgata
     6541 ccaatagagg aatacttgct tgtaccgctg gttgatatgc accagaaata ccataaagta
     6601 acatcaaagc aacaataaac aaaggtatga taggaatttt tcccaatgac aaataaaata
     6661 ctaaaattat gattgctgtt acaaaatcta gtatgaccat aatgtttctt ttatttgtcc
     6721 tgtcagcaat aataccacct accagcgaca aaataaccat aggtaaaaaa gagcaggctg
     6781 tagcaatacc aaacaaagct gatgaaccag tttcttttaa caaatagtac ggtaatgcaa
     6841 atcgtaggat agcgtttcca aataaagaaa ttatctgtcc tataacaaca agtataaaat
     6901 cacgattaaa aagttttttt tctgcttgca taaaatctct cctttttatt taatcaaaca
     6961 gaccgtcggt ttatataatg gtttaaataa actgctcttt tatagagcag tctatcgtat
     7021 agggataaca aatacagtta tttacaaaac tgtataaatg cagaaattgt ttcttcgtct
     7081 aataatgtgt ctatgcctat tccatttaaa agggcattga atgtattgca acgtttttcc
     7141 ataccctctt tatccgtcat ttcaactaat gggttgagcc atacattact taataccatg
     7201 ataacttctg caatttcatg tgaattttca atctccaaag acttatcctt gataccttgt
     7261 tctaaaacgg gttcaacaaa ttcaggtact acaatttcat acatttgttc tatttgcata
     7321 gcaagaaaac gaggattgtt taatagttga ggagaaactg tcaataaaac gctttggtta
     7381 gaattgaata ccgctgtctt aaatatttct cttaactttt ctaaaccgtt taaactatcg
     7441 ttatctctaa ttttagcaaa tattactgca ttttcatttc caattctttg aaaaattgct
     7501 tttaaaatat cttcctttga atcgaaatga tgataaatag cacctttaga aagttttgtt
     7561 tcattgataa tatcttgcaa tgatgtgtta tcatatcctt tttcacaaaa catttttgta
     7621 gaaacatcta aaatgagttt tactgtttct tctggatatt tatttcgtgc cataacttac
     7681 ctccaataca aaccgttggt ctgattaaag tatatcttag tattttatgc ttgtcaatat
     7741 aaagacttgc aattttataa aaatgcagaa tgcaagcctt tttcatttat tctacaatct
     7801 tccagttgct ttccttatgc ggtgtcaatt catactgtga tacctgcgtt gcctttgcct
     7861 gatttaccac aaatcagatt ttatcccttt cttcctcttg attgcccaca acagaaaaga
     7921 aaaagccctg tcaagagctt tgccaccatt ggtggtgctt tgcactcttg atcgggcttt
     7981 ttgtttgctg tatcttctcg caagaggaaa aaagttactc gtcctcatca tcaaaattgt
     8041 tattccttaa tacttctcgt aacagttcca tatcttcttt tgagttcggg tttatcattt
     8101 tcacgacttg ataaggtgtc ttatacttat gtccctctat gctttcccca aacatatcca
     8161 tactgtataa atcatcatag cggtctaata atttttgaaa cttcaaatgc tggataaatt
     8221 ctttgattgg gtaaaggtcg gacgcttcaa tgcttttccc gttgatatag tctgtaatat
     8281 attcccgtaa cttttctaac tcatattcca gccattcttc ctcgctgtca aagaagttat
     8341 catagtgtcc atgtttcagt tctgcattta gacgttcttc ggctcttaaa aactgacaga
     8401 cttccttttc agattgattg acatacttcc attctcccga caacaattca ttgggcgtaa
     8461 cgtccagcac ttccattatc ttttccagcg tttcataggc aggatagttc aaccctcttt
     8521 caatcttgga aaggctctgc atattgatac cgattttgtc cgcaagttcc tgttgtttca
     8581 ttcccctaaa ttttcttatg gtctgtatat tatttcctaa atggcttatt ttcattgcat
     8641 aaccctccat atctcgttat atcttcgcta ttataagtct atcatgctta atatgatttg
     8701 taaaggcaaa aaaagatatt gacaaataag catataagaa ttactatgtt cttgtaatcg
     8761 aataagcaca atagaatgaa atataaaaac attcaaaaag aaatacctat ctgattaaat
     8821 ccgtagatat gaatatcggt tgaaatggaa cttgttagaa aaggcaaaac agaacttgct
     8881 gacaaagtat ttctgacacg gtggcggaag cctctcaggc ttaccgccat gacaccttgt
     8941 gtcaagggga agaaatgctt tgtcggctgg cgagcctgcg agccttgtct gtgcccccgt
     9001 tatctaacag gggggcacta tttcacaaaa gacaagtcaa aaaccataca cccccaaacc
     9061 tgtgagtagt aatgagaggt tttaagaaga tagtacatat cacacttttt gaaaggtggt
     9121 ttacaggtat ttcaaggcta ttgtgggatt ggaggaatac aatgaacaat ttagaatttg
     9181 cacttgaaat gaagaacaaa cgtcttgcca gcggtctttc acaaggagaa cttgcaagtc
     9241 ttacccatgt atcaagatat agtatcaacc gctttgagaa cggaaaagct aacgccagca
     9301 aggaaacaca gaacattatc ctgcgttgtc tgaattacta tatctgcgat aagccttttt
     9361 atctgctcgt tgattatctg tctgtccgtt ttccaacgac tgacgcattg gaagttatcc
     9421 gaaaggtgct tggcatgaaa gcagattatt ttatccatta tgactatggg tattatggct
     9481 ataaggagca ttatgcttac ggggaaatca aggtcatggc ttctgatgat gaacacatgg
     9541 gcgtattttt agaattaaaa ggggcaggct cacgcaacat ggaatatgtc ttacaggcac
     9601 agaacaggga ctggtattca ttcttaaacc gctgtcttga ctgtggcggt gttatccgac
     9661 gttttgactt ggcaatcaat gatatgtgcg gtctgctgga tattcctgtg ctatctgaaa
     9721 aatacagaaa tggtggtgcg gaatgtcgct gtaaaaacta tgaaaatgta caaggtggaa
     9781 aattaagcgg aaaaaaccgt aatttagcaa gtacccttta tatcggctca aaggcaagca
     9841 caaaatattt ctgcctgtat gaaaaacaaa aggaacaggc aacgaaaaag aaacataccg
     9901 atattatcaa ccgttttgaa attcgtctgc gtgacaaaaa ggcggtacag gcagttgaag
     9961 aactgttatt gacgtataac cctcatgggc tggtgtttta tctcattacc gattttgtgg
    10021 aatttcctga ctatccattg tgggagatat tcatttccca tgacagttta ccttttgaaa
    10081 tgaaccctgt acctgtcaat atggaacgca ctctgcaatg gctggaaaga caggtcatgc
    10141 cgtcaatcgt gatgatagag gaaatcgaca ggctgacagg ttcaaactac atgaaaatga
    10201 ttgatgaatg tacccacctt tctgaaaaac aggaaatgct tgtggaacag atgtgtacgg
    10261 acatatcaga cgtaatcgaa agtgaggggg tgttttatga gtaatacaca ggatattccc
    10321 gtatgggaaa aatacaccct taccattgaa gaagcgtcaa agtatttccg cattggagaa
    10381 aacaagttaa gacgattggc agaggaaaac aaggacgctg gctggctcat tatgaatggc
    10441 aaccgcatac agattaaacg ccgacagttt gaaaaggtta ttgataaatt ggacgcaatc
    10501 taatgcaaat gagccttgta tgtgttatga tgaacacaag tcatatcaag gctctttcca
    10561 acaaggaaag gagcagacac catgaaagaa aaaagacggg ataacaaagg tcgtatcctg
    10621 catactggag agagccaacg aacagacgga aaatacttat ataaatatgt agacgcattg
    10681 ggaaacacaa aatatgtgta tgcttggaga ttgacaccca cagacccgac accaaaggga
    10741 aaacgggaaa aaccctcact tcgagaactg gaacagcaga taagacggga tattgaggac
    10801 ggtatcgaca gcacaggcaa gaaaatgacg ctttgccaac tctatgccaa acagaacgca
    10861 cagaggacaa atgtgaagaa aagcacacag aaacaacggg aacaactcat gcggttatta
    10921 aaagaggaca agttaggtgc taggagcatt gatacgataa aaccctctga cgctaaagaa
    10981 tgggcgttac gcatgaaaga caaaggcttt tcctacaata ccattaacaa ccataaacgc
    11041 tcgttaaaag cgtcattcta catagccata caagatgatt atgtgaggaa aaaccctttt
    11101 gatttcaagc taagtgaagt catagaaaat gataccaaag agaaagtcgc attgacagag
    11161 gaacaggagc aagccttatt gtcatttatt aagacggaca acgtgtatca caagcattat
    11221 gatgatgtgc tgatactgtt aaagacagga cttcgtatct cggaactgtg cggactgaca
    11281 gtagccgaca ttgatttcaa gaatgaagtt gtgattatcg accaccagtt actaaagagt
    11341 aaggaacagg gctattatat tgaaacgcct aagacgaaaa gcgggataag gcaagtgcca
    11401 ttaagcaaag aaacgataca ggcatttcaa cgggttatca acagccgacc aaagataaag
    11461 tcgatagagg tagacggaca cagcggtttt gtgtttatca atccgaaagg taatccaaaa
    11521 gttgccattg attataacgc cttatttatc cgtatggtaa agaaatacaa caagcaccac
    11581 aaggacaatc ccttgccaca tattacaccg catacgctac gccatacatt ctgcacaagg
    11641 ctggcaagca agaacatgaa cccgaaagat ttacagtata tcatggggca ttcaaacatc
    11701 agtatcacaa tgaactggta cgctcatgcg tccatagata ccgcaaaatc agaggttcag
    11761 cgtctaatcg catagaagta tttaccacga ttttaaccac gattgacagg gaaaatataa
    11821 gaagatagac ctagatatgc gaggtttacc acaaaagcaa aatgcccgta gagccgataa
    11881 aataaggctt tgcggacatt tgagaagata taaaaagata gtcaaaaaga catatataat
    11941 ttataataat tatgactctt aattaaagta ggtgcccata tgcttgtttc ttttagtatg
    12001 gttataagat tttttctgac aattttatta tcactgatat taacacctgt attcaaaatc
    12061 atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa gaagaataaa taaagttcca
    12121 atgccttcag cgggcggact gcctatcttt atttcatttg ttatatcgac tttattctta
    12181 ttccgtaaaa ttatccctca atcgtatatt gtaccgatct tgattgcttc tttagtaatc
    12241 atagtcacag gagtgctcga tgataaatat gagctgacac ctaaacaaaa aagtataggg
    12301 atcttgattg cttctcttat tatttatttt gtagcagata tacgtattga ttccttcacg
    12361 cttcctttta ttggatatat ccaactaggc tggcttagtt ttcccgtaac gattttttgg
    12421 atcttcggta tcacgaatgc ggtcaatctg attgacggtt tggatgggct ggcggcaggg
    12481 atttcattga tcggtttaat tacgatagga atcattggat attttttcct ccatgcttct
    12541 acagtgtata ttccagttgt tattttctgt cttgtagcaa gtattatcgg tttttttcct
    12601 tacaattttt atccggcaaa gatttattta ggcgatacag gagctctatt cctagggttt
    12661 atgatggccg tcctttcact acaagggctg aagaacgtga cttttgtttc atcgatttct
    12721 ttgctgatcg tcatgggagt gccggtaaca gacacgtttt ttgctatcat taggagaaaa
    12781 gcaaaccgtg tttctttttc tacagcagat aaaaaacatc ttcatcaccg actaatcagt
    12841 ttaggcttta ctcataaagg tgctgtcctg acgatttatg cgatggcttt gatgttttct
    12901 ttcacagcta tggtcatgaa ttatactgga agaatcggaa cgatcatatt gattattgcc
    12961 atgttgtttg ctgcaatttt attatttgaa ttgattggtc tgatcaatga gaaacatcag
    13021 tttattttaa aaacattgcg atttttaggg aataaagagt accgtacgca agtaaaacag
    13081 aaatatttga agaaatggaa ccgtcatata gatgaataac aaattaaacc agccgatcct
    13141 tattagtatt gtgacggcaa acagtgataa aatttttcgg acactagata attttatttc
    13201 aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt gacaacaatt cacttcccga
    13261 gtacaaaaaa agactaacag agtataggaa atatccctac atcgatatct attttcatga
    13321 tgaaaattcc ggatttggtt atggacataa tcacaatctg atagctagcg attctcgcta
    13381 tggcattatt tgtaacccag atattttagt ggatgaagag acagttacag cattggtctt
    13441 gttattgaaa gaacatccgg aatgtgcgat gttagcacct aagattctta atgaagatgg
    13501 aacaactcag cacctcatcc gtgagcagct ggctgttttt gattacgttt tgcgctttgt
    13561 gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg gctgtgtttg aatgccgagg
    13621 attgcctact gatgctaata gttatgtacg gatgatctca ggtagtttta tggtagctga
    13681 tattgataaa tttaaagaga taaaggggtt tgatgagcgt ttcttcatgt actttgaaga
    13741 caacgacttg tgtttgagtt ttgaaaaagc aggttacaag ttattgtaca caccctttta
    13801 tgcagttatc catttttacg ggaagggtgc gcatcgaaat gcaaaactgt ttcgcgtgtt
    13861 tttgacttct atgatgaaat ttttcaataa gtgggggtgg cgttttttct gatgaaacca
    13921 gaaatattag tagtcgttgt gctttatcag caactattca gtcaatctcc agcttatgga
    13981 ccattaagca aagcacttag cgaaaagcaa attcagctta ttatttacga taatagtcct
    14041 caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg tttatcatca tgatccgcaa
    14101 aatcctggtt tagcggtggc ttataatttt gcgttgtctc aagcagatga aggaacacgt
    14161 tgtctcgtca ctctggatca ggatacgagg ctagtcgacg attattttga actattgcgt
    14221 aaagtaacct ttacagatga atgtgttgct gcagttccga tgattttttc tgggagccga
    14281 caagtttctc ccctatattc agatcgttat atcaatcgta cagctcaagc agtagaagtt
    14341 gaaggcccaa cagctgaacg aatcatggca atcaattcag gtgttgcctg gtctgtcaaa
    14401 tttctaaaaa agattggcgg gtttaatcct gctttttctt tagatttttt ggatcattgg
    14461 ctgttttgga aagtggatca gttggaaaaa actgtggaga tccttcctgt acgaatggaa
    14521 catgatcttt cggttttaga ctatgaaaaa gtttcaagta atcgataccg ttctatttta
    14581 caagcagaaa atcgatttta tcatgaatat gatcaaaggc atttatccag tcaccgacga
    14641 caattattgt taagaacagc taaacagttc ttgaccgtca aaaaccggca tatctggcgg
    14701 atgactttgc gctcatatgt taagaattgg aaggtgtgaa ttcgtgcgtt cggtttgtat
    14761 agctaccttt aatggaggca gatatttaag agagcagtta gatagtattt tatctcagct
    14821 ttcagcagaa gatgaagtag tcatttctga tgatgggtca gtagatgata catgggatat
    14881 attaactgaa tacgcagaaa aagataagcg aatccagctt ttttcggggc ctcgtcaagg
    14941 attgatcgct aattttggtt atgcgatcca gcagacgaaa ggagatctaa tctttttggc
    15001 agaccaagat gatgtttggt tggagacaaa agtgaaaaag atcactgact attttgagca
    15061 gaatccggat cagctagtag tagtttctga tttaatcatt gtggacagcg agctgcaaga
    15121 gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa ggatggctta ataatgtgat
    15181 aagaagtcac tatattggtg ctggtatggc tttccggtct tctttaaaac ccgatatttt
    15241 gccgatcccg tcagaagtgc cgatgcatga tatgtggatt ggtttgctgg gaggaaagaa
    15301 gactgggttc ctaagagaac cgttaactct gtaccgaaga catgatttaa acgcgagtga
    15361 gattgaaaca cattctaaat tacggcaaaa aatttcttgg cgtgttcatc tgattacggc
    15421 gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg tttaaatcct taatatttcc
    15481 atgacaaata taacaattga tgagataata acaaagtgaa atttgaaatc aaaaaggaga
    15541 gcagatatcc atgaagggaa tcattcttgc tgggggcagc ggaacacgtc tgtacccttt
    15601 aacaaaggca acatcgaaac aattgatgcc gatttatgac aaaccaatga tttattatcc
    15661 aatgtctact ctgatgttgg cgggaatcaa agaaattctg attatttcaa cgccacaaga
    15721 tacaccacgt ttcaaggaat tatttggaaa cggaaatgat ttaggactgc agatcgaata
    15781 tgcagtacaa gaaagtccag atggattggc acaagcgttt atcattggag aagaatttat
    15841 tggagatgat agtgtctgct tggtattagg ggacaatatt tactatggtg gcggtctgtc
    15901 taaaatgttg caacgagcag cttcaaaaga tacaggcgcg acagtatttg gctatcatgt
    15961 caatgatcca gaacgctttg gtgtagtaga atttgatgag aatatgcaag ctttgtcgat
    16021 tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta actggccttt atttttatga
    16081 caatgaggta atttctatcg ctaaaggtat caaaccttca gaacgtggag aactagagat
    16141 tacggatgtc aataaagcat atttggataa aggaaaacta tccgtagaac ttatgggacg
    16201 cggatttgct tggcttgata caggaacaca tgaatcattg cttgaagcat ctacctttat
    16261 cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt ttggaagaaa ttgcttaccg
    16321 tatgggctac atcgataagg aacaattgct ggcccttgca cagccattga aaaaaaatca
    16381 atacggtcaa tatttattga atttggccgc tgaataagga gtccttacag atgaaagtaa
    16441 cagatacaaa attaacagat gtcaaaatcg tagaaatgga cgtctttggc gatcatcgcg
    16501 gtttcttcac agaaagttat tcaaaagcaa aatttgcaga acatggattg aaatatgact
    16561 ttgttcaaga caaccattca ctatcagctg aagctggtgt ccttcggggg cttcatttcc
    16621 aaaaaggaga agctgcacag acaaaattga ttcgggtagt cactggagca gttttagatg
    16681 tagtagtgga tatccgcaaa ggtagcccga cttatggtca atgggacgga tttatcttat
    16741 ctgaacataa tcatcgacag ttgcttgtac ctaaaggata tgcccatgga ttcgttactt
    16801 tgacaccaaa tgtgaacttc ctttataagt gtgataatta ctataatgca gaagcagatg
    16861 ggggcatcgc atttgatgat ccagatttag cgattgattg gccaattgat ccatcaagag
    16921 cgattatgtc ggaaaaagat caccgtcatc cgacattaaa agaatttgaa gcagaaaatc
    16981 catttgttta cggagaaatc taagaattaa ggagaaattt tcttatgaaa aacatcattg
    17041 ttaccggagg tgccggattc atcggctcta atttcgttca ttacgtggta aacaatcatc
    17101 cagaagtgca tgtcactgtt ttggataaac tgacttatgc aggaaacaaa gagaacttgg
    17161 ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga tgttgcagat gcagaattag
    17221 tggaccgtct agtaaaagaa gcggacgcag ttgttcatta cgcggcagaa tcccataacg
    17281 ataattcatt aaaagatcct ttcccatttg tacaaacgaa tatcattggt acgtatacac
    17341 ttttagaagc ttgccgcaag tatgatgtac gctatcatca cgtatctact gacgaggttt
    17401 acggcgatct tccattacgt gaagacctgc caggacatgg ggaaggcgaa ggagaaaaat
    17461 tcactgcgga aacaccatat aatccttcaa gtccatattc ttctacaaaa gctggttcag
    17521 atctgttagt aaaagcatgg gtgcgttctt ttggcttacg tgcaaccatt tcaaactgtt
    17581 caaacaacta tggtccttat cagcatattg aaaaatttat tcctcgccaa gtaacgaata
    17641 tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa gaatgttcgt gactggatcc
    17701 atacgaatga tcattcttca gcagtgtggc tgattttgac gaaaggcaga attggcgaaa
    17761 cctatctgat cggtgcagac ggtgaagaag ataataagac cgtgatggaa ttgatcttag
    17821 aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa cgaccgtgca ggacacgact
    17881 tgcgttatgc aatcgattcg acaaaattac gggaagagtt aggatggaag cctgagttta
    17941 cgaacttccg tgatgggttg gctgacacga tcaaatggta cgaagaacat gaagactggt
    18001 ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa aaatggacaa taatacagag
    18061 aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag gcgctcgcct gtctcttttt
    18121 taaaataaaa atcaaggagc ggataatcat atgtttttat taactggagg aaatgggcaa
    18181 ctaggtacag aattgcgcca tttattagat gaaaaaaatc tgcaatatgt ttcaacagat
    18241 gcaaaagact tggatatcac agatgccgag aaaacaatgg cttacatcac agaattaaaa
    18301 cctgatgtta ttttccattg tgcagcttac acagcagtag ataaagcaga agaagaagca
    18361 aaagaattag atgagaagat caatgttgat ggtacaagaa atgtagcact tgcagcaaaa
    18421 gctgtgaatg ctcaattcgt ctatatcagt acagactatg ttttcgatgg aaaaaacaaa
    18481 aatgtagaat acgaagtaga cgaccaaacg aatccactaa atgaatatgg tcgtacgaaa
    18541 ttattaggcg aaaaagcagt agaagagatt ttagaagact attatatcat acgaacttct
    18601 tgggttttcg ggatctatgg acacaatttc gtcttcacga tgcaacgcct agcggaaacc
    18661 cgcgacaaac tgactgtagt aaatgaccag tttgggcgac caacttggac acgtactttg
    18721 gcagaattta tggtatatgt catcgaagaa aaagcaccat ttggtattta tcatttgtcg
    18781 aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa tattgaaaga tacagatgta
    18841 gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag caacacgtcc gcagtattca
    18901 gtcatgagcc tgaaaaagac agaagagtta ggatttgtca taccaacttg gcaagaagca
    18961 cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt aatttccttt tatcaaatag
    19021 aatttggagc atgatatgga aaaacaaaca agaagtgcac gacgaaaaga aaagccaaaa
    19081 gcgaaccagg cagattcgaa tttggtgctt gacctgtttc taaaactccg ctatcttatt
    19141 ggcgttttag tcatcgtggt catcgttact tttaatttaa acggaagttc acttggtgtt
    19201 tgggataaat atgtgtcgca aagagacgat gggaaaaaaa ctgatgtgat ttttggacag
    19261 aatcgggaag tgcggtcaga cgaatggctg gtgcagacac ctttttacct ctcacaagct
    19321 gaagaggact ttccgttggt gaacgaggat tattcattaa acggacaaaa catgatcatt
    19381 gcgtataatt ctcctgtaaa ggatattacg gttatcggaa aaccattcaa ctggggattc
    19441 tttttcctcg gaagagatcg aggactttcc ttttattggg cgatgaaatt agtcgtcatg
    19501 gtcttgcttg gattcgaagt actgatgata ttaacgaaga gaaaaaaagc tctatcgcta
    19561 atcggtgcat ttgcgctgac attttctcca gcagtccagt ggtggttcat gcaacatgtg
    19621 ggcgatttga ttttctttac tttgggactg atgactgctt tttatcatta tttctacaat
    19681 catgataaaa aatggctacg aacgttgatg atgcttttgg tggttatttt cggtattggg
    19741 tttattttag tgatttaccc agcccatcaa gtgatgctgg cgtacttgct gattttttat
    19801 tttgttggcc tgcttattta ctatagcaag aaagtttcat gggatctgtt ggatgtagtg
    19861 ttgattggag gggcagtttt atttattggc ggagtcatgg ttcatttctg gctaacgtca
    19921 aaagacgcat tgttagcttc tttgaatacc ttatatccag gaaaacgggt aagtactgga
    19981 ggaaagtgga cgattggcga atttttttct ttccttacaa attggaaaat tccttttgaa
    20041 gatatcaact attcgaataa ctctgaagtg gccctgttct atcacttttt tccaactgta
    20101 ttcctagcaa gtccatttgt gttatttggc aaaaaagata gcgaacaaaa gttactggga
    20161 cgtattttga tgattttttg tctgttttct atcttttgga ttaccgttgg gttgccaaaa
    20221 tggctagcag aaatcactct gctgtcttat gttcctccat taagagccat ccttactttt
    20281 agttttgcgg cttgtttatt atcgatctgg tttatcgctt atatttggag agaagatgtt
    20341 attcctattt ggtataaatt cattttgttg gcagtaaatg catggtctta tttctatgcg
    20401 attacgcatt cgaaaatgga taattatttt tcggattttg aaaccatcgc agttgctgtg
    20461 ttgggaacag tgattttagc ttttgcatta tttggctgga gacgcttgtt ttacctcgct
    20521 tttgccgcct tagttatcgt atctggcttt ttcgtcaatc cagtcgtaga aggtacagga
    20581 gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa tcgatcaaca agatcctggt
    20641 aaagtttggt tgtctgaaga tgaattatat aacttcaccc caacattggg agtgaaatcc
    20701 ttcaatacag tacgctttta tccagatata gctgcatgga agaaaatcga tccgaaagaa
    20761 gaatacgaga agtattataa tcggtatgct catacaagag cttttatcgc taaggaagat
    20821 acaaagttca ttttagatcg tccagatatg ttttcggtca cgttgaattt tgaagacgca
    20881 gaaaaattag ggatcgacta tgtgatcagt aaacgaccat taactgatta caaccaatta
    20941 tatgaagcgc agttcacgca gctatatgga ccggataaag acggttatat gatttttaaa
    21001 gtagaatatc cggattatcc agatgtacca caaacacaaa ctcaggaaaa tcagctagtc
    21061 gtacagtaat aggctagctg agaataatcc tgaaatgtta gtagaaagga gagtggagat
    21121 aattcaagca atcgtacaaa aatttgtcaa caaaatgagt gtcggtttct tgcttttgtt
    21181 actgacgcta gtcttttttg tttggacagc agggttaggt tttccagcaa ctttgtttat
    21241 atataacaat agttcattac ttatcgcttt tagtgtggct attttgctca ttttaaatgt
    21301 ttataaagca aaaggcgtcg attttcttta tctaggtgtt gcgcttgccc ttttcatttt
    21361 cttttcttac tttcgggaat taagaaatga gtctttattt ggagacttac tgtttccttt
    21421 gattatttta ttttgtttag taattaaatg gatcgagtgg acaaaatggg atcgcggtat
    21481 ttatctgatt gtcttttcgg tcttcctcgg aatcacgtta tatcgtgtat ttacagagat
    21541 ccaagtacca gaaggacaaa gcatctgggc tccaggcaat tcgctttctg atatttggat
    21601 caatacgaat ataatcggaa gttctttgat gactcttgct ttattgatca gtggatttgc
    21661 cagttctttt gaacgatggt atgtacgaat catcggtgca gtagcagttg ttgcagcttt
    21721 tctcgggatt tgggtctgcc aatctagagg tgcattagtc gcgttgattg tttttgcgat
    21781 attggatctt ttacctaaat cgttgatgcg tgtcattcgt gcgccattta ttgcttacac
    21841 ggttacgatt cttttagctt tgccgatttc ttatctggcg gctgtctctg aaaaagtcaa
    21901 tttgtttacc ggtcgagaag atatttggca taaattctat cagacgctag gagaaaaatc
    21961 tgaacaaatt ttactaggaa tgaaaacatt tatcttccaa cgaggaaacc aatttttagg
    22021 aaaccacaat agttataatt ctattttgaa tatctatgga ttgataggat ttgggattgc
    22081 tgctttgctg cttatccttt ttattggtcg cttaacgttg aaagcagatt tatccaacgg
    22141 gcagatgact tttatctggg ccttttttgc ggtgatgatg cagtcattta tggaggatac
    22201 attgacttca tacccatggg ttccgattat ttatatcctg ttggctatgg ccgttcatcg
    22261 atatgatgaa ccagaagttg cggaggaatt ctatgttatg gaagaggcat atgacgatat
    22321 gacttatgat gatacatctt atgaggaaca accagatagc gctcattctt ccaggatcca
    22381 acgctatcat tgataggcac aaaatacgag agaggctgtg acaaccgtcg cagccttttt
    22441 tggtttattg caattgtcta gcatcttctg taaaatagaa acgttagaga aaatcttgta
    22501 tcatagaaaa ttaagaaaga aataagaacg cagctcggta ccaaaaatat ggtaaaatgg
    22561 cacttgactg taattcactg aaaatcgctt ggaggaacac aaatgtttaa agatttgtat
    22621 ttatttatga aggacattta tcagaacaga aaattattgc tacagttttc gatcaatgac
    22681 ttcaaatcaa gatatgcagg atcgttttta ggaatcttat gggcgtttat caatccgcta
    22741 tttactgtat tgatttattg gctggtattt ggtgttggac tgaaagcaca aatgatcgat
    22801 gcaaaatacc catttatcgt atatttagtt accgggatga ttccctggtt cttcttctca
    22861 gatgcatttg catcaactac ggttgttttt cgggaatata cttatttagt aaagaaagtc
    22921 gtattcaata ttcgtatctt gcccacgacg aaaatcctat ccaatctgta cacccactta
    22981 ttcttcattc tgattggcat agctgtcgcg cttataaatg gtatatatcc ttcagcaatg
    23041 tctttacagc tgatttatta tttattttgc atgtgtgctt tcttgacagg attgacttgg
    23101 ctgacggcat cgatccaacc atttttacct gatatcatgc aattaattac gatcgtgctg
    23161 cagttgatca tgtggacatt accgatatta tggagcccta atcagtttga ttcaggtctt
    23221 gtaagcatat tgaaattaaa tccgctgtac tatgttgttc aaggttatcg tgagtcattt
    23281 ttaagtgaag cttggttctg ggagcatgga acatatactt tgtatttctg ggttttcacg
    23341 ttagtaatgt tcgtaatcgg ctctgcagtg ttccgtagat tgaaaccaca tttttcagat
    23401 gtgctgtaag gagaatagct atgacagaat atgcaattag actaaaagat atcacaaaat
    23461 cttataatat gtacgcaaaa ccttcggatc gatttcgtga agccctcaat ccttttaaga
    23521 aatcctatca tgatgtattt tatgcgttga aaaacgtgaa tgtagaaata aaaaaaggtg
    23581 agatgatcgg tttcgtggga gaaaacggat cgggaaaatc tacaatgttg aaaatcatta
    23641 cgggagtact gacaccaaca tccggcacga tggaaatcga aggaaaaatc tcagctttgc
    23701 tagaactagg atcaggattc aatcctgagt attctggcta tgaaaatatc tatttaaatg
    23761 gaatggttct aggattttca agagaagaag tcgatgaaat ggtcgatgat atcattgaat
    23821 ttgctgacat cggagaccat atttatcaac ctgtaaaaac ttattcaagc gggatgttcg
    23881 ttcgtctagc ttttgctgta gcaatcaatg tgaatcccga tattttgatt gtggatgaag
    23941 cattggcagt aggcgatctt gaatttcaac taaaatgtat ggaaaaattt acagaaatca
    24001 agaactcggg aaagacgatt ttattcgttt ctcatgatat cagttcgatt cgtcgttttt
    24061 gtgatcgttc ctattggttg aaaaacggag aagtcgtcga atatggcgat acgatggaag
    24121 taacgacgaa ttatgaaaac tttttaaaga aaaaatcagt taaaacagtg gatcggaata
    24181 aaaaccaagt agaacggttc gccgctccag atatcgtcga cgtgacgaaa gcggaattgt
    24241 tggataaaga caaaaatccg gtggatatgc tggaacaagg agaaaaacta tacgttaaag
    24301 taacttatga agtaaaagat gatacaatca aaaatccggt tttaggaatt gcgctacgaa
    24361 cagtcgacaa tttttatgta tgcggactta atactctact agatggtatc aagattcctt
    24421 ggaaaaaagg agagaatatc ttttatctgg agtatgaaaa gttaggtttg ttagctggtg
    24481 aatattattt tgacgttgcg gtctttgaag aaaatgcaac ggttcctttg gtatataaga
    24541 caaagtacat gaacttgttt gtcagcggtt cttatatcgg agaaggcatc gtggtcttag
    24601 accataaatg ggaagaaggg actcatagca atgaaatatg attttgaaat ggacctagat
    24661 gaacaaagca gtgtgggaaa aattgccgca cagatcaaac caggaagtaa agttttagag
    24721 tttggtcccg gaaacggccg attaacgaaa catttaatcg gagcaaaaca atgtgaagtg
    24781 agcattgttg agttagataa ggaattgttc gattttgtca gcgaatttgc tcaagatgga
    24841 ttttatggtg atatcgaaag ttttgagtgg gcaaactatt atgctggaca gacatttgat
    24901 tacgtcttgt ttgcggatgt tttagaacat ttggtagatc ctggaaaaac attgaagaaa
    24961 gtcagagaat ttttgaatga agaaggagag atcctgatta cattccctaa cttggcgcat
    25021 aattctgtca tgatcgattt gttcaataac caattgccgt gggcttctta tggtctccta
    25081 gatgaaaccc ataattcttt ttatactcat gatggcttcc aaaaagtttt tgaaaaagcg
    25141 ggattattca ttaatattga agattatttg tacttagctg taggtgatac agaattaaaa
    25201 tcaacttatg aagaattgcc ggaagctgta cgctatgatt tcaaaatgcg accatttggt
    25261 gaagtctacc aatacttttt ctcattgatg aaacatccag tagcacaaag ttccattgca
    25321 gagccacaaa attctaatta tgtaaaagtg ttagaagtaa cccagcaaac gaaacaagat
    25381 gaaacaatcc aacaaatacc attcaataat ttcacagggg aaaatgagac actaagtttc
    25441 cccgtatcag aaacaacaga aagaatggtg tttcgcttcg cgcaacaacc tagctttatc
    25501 gaattcagcg cagaagctgc cggagaaaaa ctaacattta tcgattcgaa tgctgtagtc
    25561 aaaacagtaa atgattgtta tttgtttgat gggaaagaat taccagagtt cgttctaacg
    25621 gatatttctg ggaaagaagt gactatccat tgtcactatc gctttatcgg ggaattgaca
    25681 ccaacaatga aagaattgtt ggaaacgatt cgtcccatgg ctgaagtcac aaagcagctt
    25741 tctgagaaaa atgacgaatt agaaagagaa aatcatcatc ttcttgagga aaatacaaga
    25801 ctagaccata cgttaaaaac aaccacaaat cgctattgca cattgttgga aagtgatgaa
    25861 tggacaataa aacatagact aggacgacgt aagaaagaaa cttcgaaaaa aatccaagaa
    25921 aaagaacttt ctatatgtat tgacgagaaa atctgggatg cagaaacaaa aatattaaaa
    25981 atcatcggat ggggaatcgc taattcggat cgccagccct tgtcctataa gttatcagct
    26041 gaccaatcgc catttttcga agcaattcct gtttcacgag aagaagtcaa tcaagcagaa
    26101 cagctcgcaa aaggaacaga agcgggattt gagttacgaa tcctttgcga gcaagaacgt
    26161 tcattcttag tggaagcagt agctcaaaat ggccaatcat ggatcattga aatgtaaata
    26221 atgattaact tataaagata aggagtaaac gaatcgtgca aaaaaaagaa attgccgtat
    26281 ttatcgatca aatcactcgt gaacgggcca ctggcgattt gcttattgtg ggctgggcaa
    26341 ttgatgaagt gaccaaagaa atccctacga taaaggtaga aaaagaaaat gtgattgctg
    26401 aagcaacaca tgtcgtaaga ttggatatca atcatttata taatcttgat gttaagaccc
    26461 aaagcggttt taaggtacgt ttatcaggga aaatgagagg aaaagccatc ttagacttcc
    26521 aaacagcgag acatcaaaat ggcatagctg tgaaattaaa cggtaaatac ccttatgatg
    26581 acggcattga atcttcatgg gaaagaaaaa aaagattact gaaaaaaggg ataaattatg
    26641 caaggactca tggcgtaaaa aaagcgatcc gtcgggtcaa attggaattg aagccaggat
    26701 cgatcgacta tgcagagtgg atcagtcgtc atgagaaacc agatttgaaa gagcaaagga
    26761 aagaagcaca aggatttgcg taccgtccat tgatttccat acttatgcct gtctataacg
    26821 ttgaaatcaa atggctcgaa aaatgcattg attcggtact ggatcaaacg tatgatcatt
    26881 gggaactatg tatcagtgat gatgcttcta ctgatccagc tatccgaaag tgtctggaat
    26941 cctatcaagc aaaagatgat agaatcaaag tcgttttccg aaaagaaaat ggacacatca
    27001 gtctggcaac taattctgca ttggaaatag cagaaggaga atttatagca ctcttagata
    27061 atgatgatga attaccgcca tttgcattat atgaagtggc aaaagtgcta aacgtacatc
    27121 cagaactgga tgtgatctac agtgacgaag ataaaatcga tgcagatggc aatcgctttg
    27181 atccgcattt caaagcagac tggtcgcctg atacattgat gggaaataat tatatctctc
    27241 acttaggcgt atatcgaaca agcattgtta aagaacttgg cggtttccgt aaaggctatg
    27301 aaggttctca agattatgat ttggtccttc gtgtgacaga acaaatccct gcagatcata
    27361 tttaccacat tgaccgagtc ttgtatcatt ggcggacgat cccaggatca actgcaagta
    27421 atggcgaagc caaaagttat atctatgatt ccggagtgaa agcattgaca gatgccctgt
    27481 ctagaagaaa catcaaagga agcgtacgtc ctggcagaat ttctggattt tatgagattg
    27541 cttatgacgt cttgcaagag gatttggtca gtgtgatcat tccaacgaag aatggctatg
    27601 aagatctaaa aacttgtgtg gattcgatca tcgaaaaaac gtcttattca aattatgaaa
    27661 tcattattgc ggataacgga agtacagatc caaaaatgca agaattattt gcagaataca
    27721 agcatcagct gaaagaccgc tttatcgtag aattgattga cattccattc aattattctc
    27781 gaattaacaa tttagcagca gaaaaagcaa acggcaaata ctttttattc ttgaataacg
    27841 atacagaagt gattgagcca gattggatga cggtgatggt ttcttatgca cagtttgatc
    27901 gaatcggctg tgtaggagca aaactgtttt atccagatga tacaacgcag catgcaggag
    27961 tattattagg aatcggtggt gttgcagggc atgcattgaa taactatgat cgaacacatt
    28021 gtggttattt cggccgattg gtcattgacg tcaattactt agcagtcact gcagcttgta
    28081 tgatggtcaa ggcagctgac ttcaatgctg ttaacgggtt tgatgaaaca ttagaagtag
    28141 cattcaacga tgttgacttg tgcttgaaag tttatgaact gggtcgttac aatgtctatg
    28201 cacatcaagc agaattgtac catttcgaat ctaaatcaag aggttatgaa gatactccag
    28261 aaaaacaaag acgttttgct ggtgaaatca aaaaaatgca agataaatgg ccagcatata
    28321 ttgcgcatga tccattttac aatgataatt taacaaaaga aggaatcggc gacttttcgt
    28381 tgagaccaga ttaaactata ataaaaagct gttatgttgt gattcgctcg cacataacgg
    28441 ctttttatag ttttttgtca aaaaaaggca gaaggtgccg actcaaaaaa ataagtcgag
    28501 gttgggacag aagtgtttaa ctccgagaaa taagaagaaa ttcacgaaaa ttgcttttca
    28561 aatttttgtg aatttcggct tatttaagaa gaagttgctt ctgctcccac cgtttattcg
    28621 tttttaaaga atgagacaaa agtgtttctt acttttgtcc cacgctctta ttcatgctca
    28681 gagcgccaat agatacaaaa tcatagccaa agtttcacga accctagctg tcaaagaatc
    28741 gatattttat tttaatatag gctattttaa aataaatggt ttaaggtatt cctctacaaa
    28801 agccactcta tcaattatca tgggtttaaa ataagaagtg atggagataa ccagattgtt
    28861 cttagcgtca atataaagaa catttccact attaccaatt gctgaatata tttcgttctc
    28921 agaatctaat acccaccata aatagccata tctcatattt tgtaagtctt ctaaagcact
    28981 ggttctaggt ttgaacatct cgtttatcca catgggtggt acgatttgtt ttcctttata
    29041 catcccttta tttaggcata atacaccaat ttttgccaga tcacttgccg ataaacacaa
    29101 accatatcct gccgtcccga tatttagtgg atcacaaaac cagacattgg ttttaggaga
    29161 cttatccatt gtaaactgct tatgttcttc agctgtttca gcataataat tgacatggtt
    29221 tggtatccct ataggatcaa atagatactt attggcaaaa tcaacagttg ttagcttact
    29281 tgtctgatac aacaagccag ttaatatgtg taaacagaca gtttgatagt tgaattcgcc
    29341 ggttatacct tttcttcctc ccaataaatc tagtgatgtt tttgtccaat cttcacttga
    29401 acagactttt gtccatggat cgcctttaca tttgtagggt gctttcattg ttagcaagtg
    29461 cttgattgta atagcgtaaa tagttttctc cccgcgtttc actttatact ctggaaaata
    29521 atctaatact ttatcatcga tacttttgat taatccttgc tctaaagcaa tgccaatcag
    29581 caaagataca atacttttcg ttgctgacat gatatgacaa gtatcgtctt ttttataacc
    29641 attccattca tctgtataaa cttctacccc atccttatat gcgacaattt gacatatatt
    29701 tggttgtcga aaaccaataa aatcatgcaa ctcttgttta ttcataagat attacctcac
    29761 tttcttctat aagcataatg tcattaaaaa tcgatttcaa ttttttcgat tcttcacgta
    29821 atacaggtat tcttatggac tagacagcgc tttgggaaga tggattctct gaaaaatata
    29881 aaggttcact ttatgagtgg ataaaaaaag cagcaaatac ttcgacttct ttaatttacc
    29941 aacattagtg aaaatcgatt catctggaga tttgtaattt agagtccttg tcttgattag
    30001 aggtgtataa agctttttat tagacatttt taaattccat tacaatttgc ttataaacta
    30061 ggtttttaaa ggtgactatg gtaagataat agagatatta ttttgtgatt aggagtgtat
    30121 atatgaataa aaatggagaa tggagtgctg cacgacggat attcattata ttaatggatg
    30181 ttattgtata caatgcagca atatatagta gtttcttact gaagtttcgt ggagagatcc
    30241 cattacgaaa tttagaaact ttccaacact cagcaattat tatttcagtg atttttattg
    30301 cgttgaacat tttgttgggt gcatatgttt tttataatcg aatgatcagt gacatcgtgt
    30361 ttgtcacagt catcggtcag gtattgatga cactagggat catggtagtg acttttgcgg
    30421 gccgctggtt tgcttttcca cgtcttgtta tcttactttc atttgttctc ggtacgcttt
    30481 tcttaagtat ctatcgtatt ctcgtatata aactttattt acgcgtaagt cgagataaaa
    30541 aggtcgtcat tattgggtta gaaaaggatg tcgcaccagc aatccaaaat tttggttcaa
    30601 agaaaaacaa caaacataaa gtcgaagcag tcattcttga taactattat gcaaacatca
    30661 aaaatatgat taaagacatc gatattgtct atcttgcttc tcatatcgaa gaaactgaaa
    30721 aaatcaaaat ctatcaatta gttactaaaa atgaaaaaaa attgtttttg aatacaacat
    30781 ttgaaaactt gacattgatc aatccaaata ttatgaattt cgaagatgaa agtattattg
    30841 aagcatctgg atttagaatc caacctgaat atgaactatt caaacgtttg tttgatatca
    30901 tcgtttcact tgttttatta gttattgctt caccatttat gttattcaca gcaattttag
    30961 tcaaagtgac atcaccggga ccaatcatct ataaacaagt gcggatcaca aaaaatcaaa
    31021 aggaattttc aatctataaa ttccgctcga tgaccgccac agccgaagca aaatctggac
    31081 cagttttagc taagagcaat gatgcacgag taacgcctgt aggtaaattt attcgagctg
    31141 tccgctttga tgaattacca caaatcttca atgtgctgaa aggcgatatg tcgatcgtag
    31201 gaccacgtcc tgaacgtcct ttcttcgtcg atcagttcaa tgcagaaaac ccttattact
    31261 acttacggca taatgttcgt gctggtatca ctgggtatgc acaagtctat ggaaaatatg
    31321 tctctgatta caacagtaaa ttacgttttg atttacttta tatcaaaaaa tattcattga
    31381 tgcttgattt aaagatcttg ctgcaaacca tcaagatcct atttgataaa atgtcctctc
    31441 aaggacttga agaagacgaa aatgtgatct cattggcaga aattgctttt ccgaatgaaa
    31501 aaatctttcg ctagttggga ggcgtaaaaa tgaacaatcc gaaattcagc ataatcgtac
    31561 cagtatacaa tgcagaagca actattggtc gaacgattga tattctaaaa gaattggatt
    31621 atgaagagta cgaagtcgtt ttagtaaatg atggatcaac agatcaaaca caggaaatca
    31681 ttcaagaact gattgaaaat gatgaacgtt ttgaattgat tactacgacc aatcaagggc
    31741 ccggattagc aagaaacgaa ggaataagac agtcaaaagg agaatatctc ttattcttcg
    31801 atgcagatga cacgccagta aaaacgatct tgtcagatta tgatcatttg ctagaaaaga
    31861 gtccaaaagc ggatcttgtt gttagttcgt ttgtttttcg tacaatggac gagggaagaa
    31921 tcgtttccga aaaagaaaac ttagttacag aatgtcacta tctaactcac gaagagttta
    31981 taaaagacat gtatcagtta atgaatgaac agttgatgta tgttgtatgg aacaaatgct
    32041 atcgtagcga tattctgaaa aataaccaaa ttctatttaa aacttataat agttgtgagg
    32101 atcgaatatt taacttacat tattatgaat actgtcatca agtgatcatg aacccaaaag
    32161 tagaatatat ttatgaattt gaaggtggga aggggatcac taatcaatac cgacctaaca
    32221 agttcgaaac atttaaagaa ttctattcat tagcaaatac tgtaacagac gatattaata
    32281 aagacggtat ggcggcgctt cttttaaaag ggacaacttc agttattttt tctatttata
    32341 gtacctctgc aagaacttca aaagagaaga aagacgaagc aaagcagata ttgagtgatc
    32401 cagtgatccg agaagccaag caaattgcta agacggattc ttcagcaaaa aaaataacaa
    32461 agttgcttta caatatgcca gctccactat ttttatctgc tgtaaaagtt ggaagcttcg
    32521 ttgaagtgaa aatgccaggg ttaatggctg ctttgaagcg agtatattag ttaatggagg
    32581 aaaatataat gaaaagaatt ttgcattttc agggacgaat gggtttaggt ggagcagagt
    32641 ctttcatgat gaatttatat cgtaaaattg atcgtacaaa ctatcaattt gattttttga
    32701 tttatgaaga ttatgcagat gttcaagact atcattcaga gattgaaaga ttaggaggcc
    32761 gtatttttgt tgtacctaat cctaagaaaa atatttttaa atatttaata gaagtgaata
    32821 agttactaaa aaaagaatct ttttctatag ttcacaatca ggtttatttt ggcgggggaa
    32881 ttaatttatg gttagccaaa aagaatggaa tcagacagag aattgctcat agccatgcaa
    32941 ctgaagatgg aaaatctcaa aatattgtaa tgaatgtatt gcgtaaattt ttaaccaaat
    33001 tgttgttaca aaatgcaaca gactaccttg ccgttagtca agaagctggt gaaagtctgt
    33061 ttcaaaacca cccctttgag atagttcata atggtattga tttagagctt tatagtaaaa
    33121 attcagaagc taaggttaat aagagaaaag aattggatat ctcaatgagt acttttgttg
    33181 tgggaaacat tggacgatta gagaaacaaa aaaatcaatc ttatttgtta gagatttttt
    33241 tagaaatact aaaaatgaat tctgattctt acttgatttt agtaggtgaa gggtcactta
    33301 gaaataaact gaaagccaaa gcagtagaat tggatataga gaaaagagta aaatttttag
    33361 gagaaagaaa cgatattccc gagttattat ctgtgatgga tgtttttgta atgccttctc
    33421 tttatgaagg tctgccaatg gttggattag aagcacaagc agcaaaatta aaattagtct
    33481 tagcagatac tatttccaaa gatacaaagt taacaaataa tgtggatttt gtatcattag
    33541 atgaaacacc acaagaatgg gcaaaaaaag taataaaaga gccgtatgct aataaaataa
    33601 cacaagaact aaagagttat gatgtttcgt acactactga acaaatggag agtatatata
    33661 gtagagatca ttaaaaatgt tcaagatatt ttaggagtga ttatttttga aaatatgtta
    33721 tttgatattg gcatacaatg atattggcaa cttaaagaga ttggtcaatc gattgaacga
    33781 aaatgcagtt tttatt
//
LOCUS       DAYYGS010000023        33678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000023 DAYYGS010000000
VERSION     DAYYGS010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.023"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..1823
                     /locus_tag="ACHA44_001024"
     CDS             162..1823
                     /locus_tag="ACHA44_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8904333.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(1893..2240)
                     /locus_tag="ACHA44_001025"
     CDS             complement(1893..2240)
                     /locus_tag="ACHA44_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HFM8904334.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2324..3265
                     /gene="opp3b"
                     /locus_tag="ACHA44_001026"
     CDS             2324..3265
                     /gene="opp3b"
                     /locus_tag="ACHA44_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8904335.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3266..4309
                     /locus_tag="ACHA44_001027"
     CDS             3266..4309
                     /locus_tag="ACHA44_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904336.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4315..5385
                     /locus_tag="ACHA44_001028"
     CDS             4315..5385
                     /locus_tag="ACHA44_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904337.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5375..6328
                     /locus_tag="ACHA44_001029"
     CDS             5375..6328
                     /locus_tag="ACHA44_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904338.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6340..7350
                     /locus_tag="ACHA44_001030"
     CDS             6340..7350
                     /locus_tag="ACHA44_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease TnpB family protein"
                     /protein_id="HFM8904339.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7635..8456)
                     /locus_tag="ACHA44_001031"
     CDS             complement(7635..8456)
                     /locus_tag="ACHA44_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFM8904340.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8553..8831)
                     /locus_tag="ACHA44_001032"
     CDS             complement(8553..8831)
                     /locus_tag="ACHA44_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904341.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9099..11108
                     /gene="metG"
                     /locus_tag="ACHA44_001033"
     CDS             9099..11108
                     /gene="metG"
                     /locus_tag="ACHA44_001033"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFM8904342.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11587..12174)
                     /locus_tag="ACHA44_001034"
     CDS             complement(11587..12174)
                     /locus_tag="ACHA44_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904343.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12349..13671
                     /locus_tag="ACHA44_001035"
     CDS             12349..13671
                     /locus_tag="ACHA44_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8904344.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13826..14602
                     /locus_tag="ACHA44_001036"
     CDS             13826..14602
                     /locus_tag="ACHA44_001036"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8904345.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14595..15164
                     /gene="rnmV"
                     /locus_tag="ACHA44_001037"
     CDS             14595..15164
                     /gene="rnmV"
                     /locus_tag="ACHA44_001037"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HFM8904346.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15157..16041
                     /gene="rsmA"
                     /locus_tag="ACHA44_001038"
     CDS             15157..16041
                     /gene="rsmA"
                     /locus_tag="ACHA44_001038"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFM8904347.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16492..17121
                     /locus_tag="ACHA44_001039"
     CDS             16492..17121
                     /locus_tag="ACHA44_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904348.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17245..18354)
                     /locus_tag="ACHA44_001040"
     CDS             complement(17245..18354)
                     /locus_tag="ACHA44_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904349.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18563..18985
                     /gene="mgsA"
                     /locus_tag="ACHA44_001041"
     CDS             18563..18985
                     /gene="mgsA"
                     /locus_tag="ACHA44_001041"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFM8904350.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     regulatory      19172..19270
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19308..19844
                     /locus_tag="ACHA44_001042"
     CDS             19308..19844
                     /locus_tag="ACHA44_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HFM8904351.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(19884..21647)
                     /locus_tag="ACHA44_001043"
     CDS             complement(19884..21647)
                     /locus_tag="ACHA44_001043"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904352.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21650..23365)
                     /locus_tag="ACHA44_001044"
     CDS             complement(21650..23365)
                     /locus_tag="ACHA44_001044"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904353.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24144..24368)
                     /locus_tag="ACHA44_001045"
     CDS             complement(24144..24368)
                     /locus_tag="ACHA44_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904354.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24559..27336
                     /locus_tag="ACHA44_001046"
     CDS             24559..27336
                     /locus_tag="ACHA44_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8904355.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27417..27836
                     /locus_tag="ACHA44_001047"
     CDS             27417..27836
                     /locus_tag="ACHA44_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8904356.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27848..28318
                     /locus_tag="ACHA44_001048"
     CDS             27848..28318
                     /locus_tag="ACHA44_001048"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8904357.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28335..29114
                     /locus_tag="ACHA44_001049"
     CDS             28335..29114
                     /locus_tag="ACHA44_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8904358.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29101..29922
                     /locus_tag="ACHA44_001050"
     CDS             29101..29922
                     /locus_tag="ACHA44_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8904359.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            29939..30988
                     /locus_tag="ACHA44_001051"
     CDS             29939..30988
                     /locus_tag="ACHA44_001051"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8904360.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31001..32005
                     /locus_tag="ACHA44_001052"
     CDS             31001..32005
                     /locus_tag="ACHA44_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8904361.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32638..33657
                     /locus_tag="ACHA44_001053"
     CDS             32638..33657
                     /locus_tag="ACHA44_001053"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFM8904362.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        10998 a         5973 c         6884 g         9823 t
ORIGIN      
        1 gctatttttt ctgaaaaatt ccttgaaacc tcacgaaaaa atcattattt tatgaaaaag
       61 acaaaaaaaa gtgttgattt tttaataatt attcattaaa ctcttaaaca acttcagaaa
      121 atattaatta ttctgaaaat tccaataaag gggtacgtaa aatgaaaaag aaatttacat
      181 ttggtttggt tgcggtatgc ggccttgtac ttgctggatg ttatggcggc agttcagcca
      241 caagcagtag ttcagcaaaa agcggttcag cagatggagg aggcgtattt aacttagtcg
      301 tgcctcaaga aatgcctaca gctgacttat cagttgcaac agatacaatc agttttacag
      361 cattgaataa cgtttatgaa ggaatttacc gtcttgatga agacagcaaa ccacagccgg
      421 caggagctag cgagttggca gaagtcagtg aagacggatt aacttacaaa atcaaattac
      481 gagaagacgc aaaatggtca aatggtgatc ctgttacagc agcggattac gtttatggct
      541 ggcaacgaac agtagaccca gctacagctt ctgaatatgc ttacctgtat gcgccagttg
      601 aaaatgcaga agacattaca gctggaaaga aagataaatc agaactaggg atcaaagcag
      661 ttggtgacta tgaattagaa atcaaattga cgaaacaaac accatatttc caatatctgc
      721 ttgctttccc atcatttttc ccacaaaaac aatccgtagt ggaagaaaat ggggatgcct
      781 atgcatctgc aagtgataaa gctgtttata atggaccatt cacattagaa gattttgatg
      841 gaccaggaac agatacagaa tggacttata agaaaaatga tcattactgg gacaaagata
      901 cagtaaaact ttctgaaatc aaagtcagtg tagtaaaaga atcttctact gcattgaact
      961 tattcaaaga tggccaagca gatgacgtga tcttgtctgg tgagttagca cagcaaaacg
     1021 caaacgatcc agcttatact tctgttaaag aagcgcggac aagctatatc gaattcaatc
     1081 aacgtgaaaa gaattcacca ttcaacaatg tgaacctaag aaaagctatt tcttattcta
     1141 tcgatcgtga agctttagta aaacaagtac taggtgacgg atcagttgtc tctacaggat
     1201 tgatcccatc agatatggta aaaagtccaa caacgaacga agactttgca aaagaagctg
     1261 gaaaattagt ttcttatgac aaagataaag caaaagaata ttgggaaaaa gcgaaaaaag
     1321 aattgggcgt tgattcttta gaatttgatt tgatggcttc tgatgatgat tcatcgaaaa
     1381 aagtaatcga atatgttcaa aactcaatcc aagaaaactt ggatggcgta acagtcaaac
     1441 caacacctgt tccattctct gtacgtcttg accgctcaac ttcgggtgat tttgacgcag
     1501 tactaggcgg atgggctgct gactatgcag acccaagcag ctttactgat ttgtttgtca
     1561 caggtaattc atacaaccgt ggtcgctgga gcaatgcaga atatgacgaa gcagttaaag
     1621 cagcttcatc aacagacgct ggcgatgaac aagcccgttg ggatgatttc caaaaagcaa
     1681 tcaagatcat ctctgatgat atgggtgtcg ttccggtgta ccaaaaagca gaaggtcacc
     1741 tagtgaatga aaaaatcaaa ggtatcgttc accatgcggc aggcgcttca tgggattaca
     1801 aatggacata cgttgaagaa taatcgttaa taccgtgagc atgagaaaaa acgaggctgc
     1861 ggcaaatgtc gcaacctcgt tctcatattc acttatccta acaaagaaaa aagcatgaaa
     1921 atagcagaaa gagcaattag tattccggca atgatcagcc aaaagctgta cataccgtag
     1981 ctggcagacg agagcgagga aaattctggt tttttctttc ggttccttgt tcgtgcaaga
     2041 tggaccgatc gctgaaaatg atcgaagaaa cccgaagaaa agacccataa aaaaccgccg
     2101 ataatcagaa acggcaatga ccataagaat agatcattag ataattgaat gagtgtaaag
     2161 gaatggttga tgagattttt tattaaaata accagaatca gtagaaaagc tactgcgtag
     2221 gggatagatt tttttttcat ttgattgctc ctttattttt ggttcattat ctaataatgt
     2281 agcgaaacat ctgtcagaag tcaaaagcta gggggaaaca ttagtgaata gttatatcaa
     2341 atatgtatta aaacgtgtct ttttcatgat catcacgctt tggttaattg caacaatcac
     2401 ctttttcttg atgcaattgc tgccgggcac gccatatacg aatcaggaac gtttgagtcc
     2461 tgaaacaatc gagatgctaa ataaacaagt cggtttggat aaaccagtta tcgtacaata
     2521 tgggatttat ctttctaatt tgcttcaagg agattttgga atttccttcc aatttaaaaa
     2581 ccagccagtc gcacatttgt tagctggaag aatcggacca tccttgcagt tagggttaca
     2641 ggcaattatc tttggaacag tcctagggac gattttagga acgatctctg ccatgaagca
     2701 aaatacatgg gcagatacat cttctacact cgtagcgata ttaggtcgtt ccattccaaa
     2761 ctttgtattt gctgttttat tacaatatat ctttgcaatc aaattacaag ttttgccaat
     2821 cgcaaaatgg gatggctttg tgtacacaat cttaccaaca attgcgttgg ccatgtcgcc
     2881 gcttgctgac tctgctcggt ttatccgtac ggaaatggtg gaagtactgc atagtgatta
     2941 tgtggaatta gcaaaagcaa aaggattgag ccgttgggaa atcgccttta aacatggtct
     3001 tcgaaatagt ttgattccat tgatgacttt attaggaccg ttagctgtag cattgatgac
     3061 cggatcactg gtggtagaaa atatctttgc tattcctggt atcggggaac aattcgttaa
     3121 gtcaatcaca acaaatgatt atcctacgat catggctgta acgatccttt actcatttat
     3181 gctgattttt gttattttga tcgtggatct attgtacggt ctagttgatc caagaattcg
     3241 tgtttcagaa gggagccgag gataaatgga aatgatacca aagcgttatg aaacagttgc
     3301 agatatccct gcggatgaat ttcttccatt acaaaaaaat accgaagaag aacgtgaaca
     3361 gattactgca ccttctttga attttatcca agattcatgg cgccgtttaa agaaaaacaa
     3421 agctgccgtt atttcaatgg ttttactatt ggtcatcatt ttgatctctg tgatcacaat
     3481 ctttgtttct cctcatgatc caactgccca aaacgtggct tatatcaatt tgcctcctcg
     3541 tattccggga atcaatatcg atggattgaa tggaaaagca atggttggtg gtgaacttgt
     3601 tgataaatat gctcaagcaa acgtcccttc aaacgtaaat ttctttttag gaacagacgg
     3661 tttgggacgt gatgtattaa gccgcttatt tatgggaatg agaatctcgt tgctgatcgc
     3721 atttattgct gcattactag atgtaacgat tggtgtagct tacgggttgg tttctggttt
     3781 gttaggcgga cgtgtcgata acgtgatgca acgttttttg gaaatcttat caggaattcc
     3841 aaacctcgtt gtcatgattt tgatgttggt cgtttttgaa ccaggaattt tttcaatcgt
     3901 ggctgctatg gccatcacaa actggatacc aatggcacgt atcgttcgtg cacaaacaat
     3961 gaaattaaaa gatcaagaat atgttttagc tggattgaca ttaggtgaat caagaatgaa
     4021 aattgcattc aaacatattt tgccgaatat ctctagtgtc attattattc aaatgatgtt
     4081 tagtattcca tctgctattt tcttcgaagc ctttttaagc tttatcggtt taggtcttac
     4141 gcctccgtca gcttcattag gaacgatgtt gagcgaaggg tacaagacat tcctgtatct
     4201 gccttatcta ttatggattc cggcagtaac tttatcggtt atcatgattg gctttaactt
     4261 acttgctgat ggattgcgtg atgcctttga tcctaaaatg aaagagtgaa gacgatgacg
     4321 aaaattttag aagtgaaaga cttagaaatc tcttttgata cgtatgctgg aaaggtacga
     4381 gcgattcgcg gtgtcaattt tcatttgaat aaaggagaaa cattggcgat cgttggggaa
     4441 tctggaagtg gaaaatcagt gacgaccaga agcatcatgc gcttgctgtc aagcaacgcc
     4501 aatatagatg ctggtcaaat cctctttaaa ggtaacgata tcgttggaaa atcagaaaga
     4561 gagatgcaaa aaatccgtgg taaagaaata gcgatgattt tccaagatcc aatgacctct
     4621 ttagatccga cgatgcccat tgggaagcaa gtcgctgagt cccttagaaa gcataataaa
     4681 gtttcaaaaa aagagggaca aaaagctgca ttagaattgc ttaaattagt tggaataccc
     4741 gaagctgaga aacgtatcaa cagttatccg catcagttct caggaggtca gcgtcaacgg
     4801 atcgtgatcg cgattgcact ggtatgttat ccggaaatcc tgattgctga tgaacctaca
     4861 acagcgcttg atgtgactat tcaagcacag attttggagc tgctgaaaga aattcagaca
     4921 aaaatcgata catcaattat ctttatcact catgaccttg gtgtagtagc gaatgtcgct
     4981 gatcgtgtcg ctgtcatgta tggaggaaga atcgttgaag ttggaacatc ggaagaaatt
     5041 ttctacaacc cgcaacatcc ctatacttgg ggactgcttg gttcaatgcc gacattagat
     5101 agtgcgaacg atcgattgta tgctattcct ggttctccac cggatttatt gaacccgcca
     5161 aaaggagacg cattctatcc aagaaatgaa ttcgcgatga aaatcgatgc agaacaagaa
     5221 ccaccatttt ttgaactatc aaaaacacac aaggcagcaa cttggctact tgctccgcag
     5281 gcacccaaag tgacaccgcc agaagaaatc caacgtcgct gggctatctt taaagagaaa
     5341 caaaaacata cttacggtca aggaggaatt gctgatgcca aataaactac ttgaagttaa
     5401 aggattgaaa cagtatttca atgtaggaaa aaagaatgaa gtacatgctg taaatgacat
     5461 cagctttcac atttatgaag gagaaacatt cggtttagtt ggagaatctg gaagcggaaa
     5521 atccactact ggccgtacga tcattcgctt gaatgaacct actgatggtg agatattatt
     5581 cgacggtcaa gatataacga agatcaaaga taaaaaaggt ttgacgaaat tccggcatga
     5641 tgttcagatg atctttcaag atccatacgc ttcgttgaat cctcgtatga aagtcagaga
     5701 tatcattgcc gaaggaattg atgttaatgg actagctaaa tcatctgaag agcgtgcaaa
     5761 aaaagtcgat gatctattga gaactgttgg attgaatcca agccacggta ctcgttatcc
     5821 tcatgaattt tcaggtgggc aacgccagcg tattggtatt gcacgagcat tggcagtaaa
     5881 accgcgtttt atcatttgtg atgaaccaat ttctgcacta gatgtgtcga tccaagccca
     5941 agtggtcaat ttgttgcaag atctccaaaa agaacatcaa ttgacctatc tatttattgc
     6001 acatgatttg tcgatggtta aacatattag tgaccgaatc ggtgtaatgc ataacggctt
     6061 gttattagag atgggaacaa gtgatgaaat ctataatcac ggtgttcatc cttatacaga
     6121 aagtttgtta tctgctattc ctctaccaga tccagaccat gagagaaaac gcagaagaat
     6181 caaatatcaa cctgaaccag atgacggaca agtacgtcaa cttcgtgaaa tcgcaccaga
     6241 acattttgtc tatgcaactg agcaggaagt cccatattac gcaaaaaaac taaaacgaca
     6301 aaaggaatct cttctggttg ctaactagga gggacataga tgctaaaagc ttttaaattc
     6361 cgtatgtatc cgacagaaga acaaaaacag caattgattc gtacttttgg atgtgctcga
     6421 tttacctata accatttgct gaaaaagcgg cagaaaagtt ggcaacaaac aggagtagcc
     6481 aatttttcgc tcacacccgc tacattaaaa aaagaatacc cattcttaaa agaagtagac
     6541 agtcttgctt tagcaaatgc acagcttaat ttggatcggg cttttcgcaa ttatttcaag
     6601 ggacgtgcca gcttcccaaa gcttaaaacg aaaaaaagta tgtggcaatc ttatacgacc
     6661 aacaaccaaa cccgtacgat ctatctggaa aatggctact taaagttacc aaaacaaaaa
     6721 gaactaatca agataaatca gcatcggcca gttgaaggat cgattcgctc cgctacgatt
     6781 tctgctcgat ataatgaaga attttatgtc gcactacttt gtgatgtttc gccagtaaaa
     6841 aaagaatctt tagctaaatg gatcgggatc gcctatcatc cgaagacatt gatccaaaca
     6901 tctcgacctc tagaagtaac tttaccaaaa tttcaccaga cagaagaaaa gctgcaacat
     6961 gcacagcgga aattgaatgt gaaagtccgc tccgctcacc atagaaagat caggttagat
     7021 caggcaagca attatcagaa acaaaagaga aaagtaatgg atctttatct gaaacaaaag
     7081 aaccagcgag aagattatct tgaacaatta tctgggaaat tggtcaaaca atatgactac
     7141 ttatttgtag aatcttttcc aaaagaagag gcacatgctg atttttctat tcacgactgg
     7201 cataaattaa taacaaaatt gcgttataag tcccaatggt ataataaaaa atttcttttg
     7261 atcaataccg acggagcaga agaaagcaat tccgtaagaa agagccaagt agtagaaaaa
     7321 atgggcagac attcggttat caagggataa aaaatgaatg aaactgatag gagcgttagg
     7381 gttagccttg ttaataaaaa gtcttaatga cttttgtttc taggaatttt tcttttgaag
     7441 aaaagctcac taattatgac aaaccaataa aacaatagca gaaaaagttc gtacgagctt
     7501 tttctgctat tgttaatttt ttattcagaa agtttacgaa tacatcaaaa ttctgaaaca
     7561 aaaaacgtaa ctcacaaaga gttgtttttg cgttttttct tgaatgatta ggtctgaatc
     7621 ttttgctcac aaccttattc atggtgcccc ggcttgagct gatagatttt tgaaggacgg
     7681 ccgattccta caggacgttc accgatctca tcgaaatatt ggagcatagc tttcttaaaa
     7741 ttggaatgat cgatcgtttt ataatcgatc cctaagaatt tagcaaatac ttttcgagct
     7801 tccgtaatcg tgaaatcttt acctaaaact tgtagaactt gcggctcgtg ttccatttta
     7861 ttgactacac ggttgaatgc tttcaagatg atttgactat ggtcaaaagc caaagtatct
     7921 ttgccagaag actctccagt gactaaatca agcgagatag ccacttctcc gctagataaa
     7981 tggatcttgt tactatgacg ttctagtgtg aaccagcgta cttccttggc atcatctccg
     8041 gcgattagcg gttcttctcc aataaaggct aagtagctga ctgtgacgac ccagcctcgc
     8101 ggatctcgat taggcgtact gaatgtatgc agttgctcaa tattttcttt tgaaataacc
     8161 acaccagttt cttcctttgt ttctcttaag acgctttctc cagttgactc atccttttgg
     8221 acaaagccac caggcaatgc ccatgaattt cgataaggat gcccttttct ttggatcaac
     8281 aaaactttca gctgatcttc ttctttgtta tagcacatca gtacgatatc gacagtcaaa
     8341 gagggcttgt catattctgg acgttcctgt ttttgatacc agttaagaaa ttcttcttca
     8401 cttgctttat gttcataata taatttttct tcttctttag aagcaaaacg gttcatctga
     8461 ccacctccat tgacagtaaa agtacttttt accttattgt agcagactct gaaaaaaaac
     8521 caaggattta cttgaaaaat ctaggttatc atttaaatat gaaaaataaa tggaagtatg
     8581 agattgaata aaaagaaaag cagataaaac ggcaatactt tataccgctc ttcacgaatg
     8641 gcaaagaagc cacaaataac gatatatacg tcaatgataa aacgcaaaat ccaagctgct
     8701 gtcataaaac tgttcaaatg cccatggagg ttaaaataac catacatacg aatataaaaa
     8761 tagctgaata ttaaatgtgc taatgaaaga aaagcaatac taagtgtaaa aaaaagatgt
     8821 ttcttctgca tgattgttcc agtcccttac taagatagat agtctttatt ataccacgtg
     8881 tgaggggaga aataaaaagc cgttttttag tagatatgaa acaaaacaaa aacccgcatt
     8941 tattcaacct attcatcctt ttaaaggata gattgaacgt agatagaagt cgtttatcca
     9001 gtctttttca ttttgaaaaa aagctaacag catgcttttt ttatggtata ctagcaagga
     9061 aacgaattcg catcatgcga taaaggagga agagccccgt ggcagaaaag gaaacttttt
     9121 atatcactac gccaatctat tatccaagcg gcaaacttca tatcggcaac tcgtatacta
     9181 cgattgcatg tgacgcaatt gctcgttata aacggttgat gggctttgat gtgttttatc
     9241 tcactggagt ggatgaacat ggtcaaaaaa tcgaaacaaa agcagaagaa ctaggtgtaa
     9301 aaccgcaaga atatgtagat aaaatggcgg ctgatgtcaa gaaattatgg aaaacgcttg
     9361 atatcagtta tgataaattc attcgcacga cagatgatta ccataaagca gctgttcaaa
     9421 aaatatttga tcgtttatta gaacagggag atatttactt aggtgaatat gaaggctggt
     9481 actctgtttc tgacgaagaa ttctttacag aaacacaact tgcagaagtg tacaaagatg
     9541 acgaaggtaa cgtgatcggt ggtaaagcgc caagtggaca cgaagtagaa ttggtaaaag
     9601 aagaatcata cttcttccgc atgagcaaat acgcagaccg tctattggaa tactatgaag
     9661 aacatcccga attcatccag ccagaatctc gtaaaaacga aatgatcaac aactttatca
     9721 aaccaggatt agaggatctt gcagtttcac gaacaacttt tacttgggga gtaccagtca
     9781 agagtgatcc gaaacatgtg gtgtatgtat ggatcgatgc attatccaat tatattactg
     9841 cactaggata tggctccgaa gatgaaagtc tattcgaaaa atactggcct gcagatgttc
     9901 agatggtcgg taaagaaatc gttcgctttc atacgatcta ttggccaatc atgctgatgg
     9961 cccttgattt gcctctacct aaaaaagtat ttgggcatgg ctggctattg atgaaagacg
    10021 gaaaaatgtc taaatcaaaa ggaaatgtcg tttatcctga aatgcttgtg gaacgttatg
    10081 gtttagatgc actccgttat tatttgcttc gtgctgttcc gtttgggtca gatggtgtct
    10141 ttacgccaga agattttgtt tcccgtgtca attatgatct ggcaaatgat ctagggaact
    10201 tattgaatcg aacgattgct atgatcaaca aatattgtga tggactcgtt cctgactatg
    10261 cctcacttgt cactccattt gatagtgaac tgtctacgac ggctgctaac gttgtcggac
    10321 gttaccatga tgcaatggaa aaaatggaat tcaatacagc attggctgaa atctgggtgc
    10381 tgatttctcg tgcaaacaaa tacattgatg aaacagaacc atgggtatta gcaaaagacg
    10441 aagaaaagaa agctgaattg aatagtgtca tgatccattt agctgaatct ttgcgtatcg
    10501 cagcgatatt acttcagcca atcatgacag aaacgccaga gaagatcttc aatcaactag
    10561 gattggactc tgaaacaatg aatcttgaag gattacactt tggcgaattt ccttcaggaa
    10621 caaaagtagt tgcaaaaggt acaccaatct tcccacgttt agatatggaa gaagaagttg
    10681 cttttattca agaaaaaatg tctgaaggta ctcaaacaaa cgaagataca gtcaaatggg
    10741 atccagaaga aacagaactt gtttcaacga aagaaaaaca aatcaaattt gatgtatttg
    10801 aaaaagtaga actaaaagta gcagaagtca tcaactgtca aaaagtagaa ggggcagata
    10861 agttgctgca attccgacta gatgctggag acagccaaga ccgtcaaatc ttgtcaggaa
    10921 tcgctgaatt ttatcctgat ccaagcgaat tgatcgggaa aaaagtcgtc attgtagcaa
    10981 atctgaaacc acgaaagatg cgtggacaaa tcagtcaagg aatgatcctt tctgcagaag
    11041 caccagatgg ctctttacaa gtaatcgaag caccaaaatc catgccaaat ggctcagaaa
    11101 tcgcatagaa attcaaaggg ggtatgacac tagttgtgtc ataccccctt tttccgaaat
    11161 ttgagaagca attttcgtaa attccttctt attacttgaa gccaatcata taatacagaa
    11221 acagagaact atccaaaata aatcctagaa aaagaagtac gagcttcgct gatttttgtt
    11281 aatttagaat aatactaata ggaatcgtaa aaacagaaaa aaagcataat gataaagggt
    11341 cgggcagatt ttaattgatt catctggtac tcttttttat tgtccttaca ttcataaagt
    11401 ttgaatgcaa tgactttatt acatctatat ttatatctaa cagtagcgtt gatgtgttaa
    11461 ttataagttc tatcacttta tgttcaatta gtttgtgctc ttatatgtgt gattgaaaat
    11521 atagataaaa aaacaatcat cggctttgac ttcgatgatt gttttcttta ttttgtaaaa
    11581 atcctatcac ttgattttat ggtaaccttt tatttttgtc ttcaaacggc ttggtaagag
    11641 agcagtaagc agtgcaaatg ggatcgaaaa gacataccag aaaatcgtac gtccgatacc
    11701 ataatgttga tagagctttg tcaacatcac gtgtaacaag atcaacccta aaagcaagga
    11761 gccagctata aaccaatgat tatcccaatg catgacttca ctaagtgcca tcactccaaa
    11821 aggatagaaa taaaaataga tcccgactaa ggtcatccgc cagttctttg taaatgttcc
    11881 gatattcaat aaaaagaaaa tcgctgaaag caaaataccg ataggcggaa aaagcccaac
    11941 caaaaaggaa tacaacatcc cccacaagat catcacagga cctttgatca atgcaacgat
    12001 tgcgatgaac aaagtcagca atattagttt ttgtgataac gttgcaacag tcattaatcc
    12061 aaataaaatt aatgtgaacc aatgccaatg ggactgctgt tgttctttac gggaaaaatc
    12121 gacccggttg gataaataat ctgctaattt cggataagaa cgcttaaaat ccataaactg
    12181 ttcctccttc gtggaaaagg taggtttagt atacgaaaaa taaaaaagaa atacatccgt
    12241 cttaagaggg agtttttcac tgaaagtgga gaaaaaagaa tggtaaatta aaatattctc
    12301 agttatactg aaaattatac caaatttcaa agaagaaaga ggatgggaat gaaaggcaaa
    12361 ctaaaacgag aaatcaatct gtttggcgca ttagctaccg ttatgggaac cgtgatcggt
    12421 gcaggcgtct tcttcaaaac tgctgctgtt actgctagta ctcagtctac aagtttgaca
    12481 ttgctagctt ggcttttggg tggtttctta acgatctgtg ctggattgac agtggctgaa
    12541 ttagctacag caatcccgga aactggtgga gctgtaaaat atattgaagc tgcgtatgga
    12601 aaattgccca gctttttatt aggatgggcg caaagcttga tttactttcc tgctaatatt
    12661 gctgcattat cgattatctt tgcgacacaa ttgacgaact tattacaatt atctaccgat
    12721 tatcttttgt tgattgcagt gatcaccgct gtttctgtga cagggttgaa tttgttaggg
    12781 acaaaagtcg gtgcttctgt acaatcagtg acgttgattg ttaaattaat accaattgct
    12841 gtgattgtca tatggggatt attgacccca ggacaaggaa ctgtccagtt atttccaatc
    12901 gaagctggaa aagatgttac ttttgtcgaa ggattgagca gtgcgttgtt agcaacgttg
    12961 tttgcttatg acggctggtt aggtgtcggt gcaatggcag gggaaatgaa acggccggaa
    13021 aaagatttgc ctaaagcaat tattcttggt ttgagttttg taacagtcgt ttatctattg
    13081 atcaattttg tttttttgaa aactttgccg attgatcatc ttgccggtaa tttgaatgca
    13141 gcttctgaag catcagacgt catttttgga ggaatcggtg gaaagctggt cactattggg
    13201 atcttgatct cggtatacgg tgcattgaac ggttatacac taactgggat ccgcgtgcct
    13261 tatgcgatgg ctttagaaga cgagctacct ttcagtaaac agctgacaaa gctttcaaag
    13321 aagtttacgg ttccttatgt tcctgcggtt ttccagttag cagtagcttg tattatgatg
    13381 agccttggtt ctttcgactt tttaacagac atgttgattt ttgtgatgtg gctattcagt
    13441 ttactgattt ttatcggtgt ctttatttta agaaaaaaag cgccggaatt gccacgtcca
    13501 tataaagtac cactttatcc aatcgttccg attattgcga ttctcggtgc tattttcatt
    13561 ttaggtatga cgatgatgac gcaaacgaaa ttagcattga tcggtatagg tgttaccttg
    13621 ataggtatcc cagtctatta tcaaaaaaag aaaaaacgct cagaagagta aaatatttca
    13681 acaacagaca gaaaaagtac gaactaggtc ctctagatct tcgtgctttt tctatttcaa
    13741 taaaacaagt tcttctattt tgatttttgc tacttctgca agtagtgctt atgtgctaaa
    13801 gtaagaatga aaaggagaga taaacatgat atttgattct catacccatt taaatgctga
    13861 acagttcaat gaagatattc cagaaacgat tcagcgagca caagaaatgg gggtaacgaa
    13921 aatggcggtc gtgggcttcg atacgccaac gattgaaaag tctttgcaat taagccatga
    13981 ctatccaaat atatacagta ttatcggctg gcatccgaca gaggctggca gttatacgaa
    14041 agaaatcgaa aataaattac aagaacaatt gactttacca aaggttgtag cattagggga
    14101 aattggcttg gattatcatt ggatggaaga cccgaaagaa gtccaagata aagtatttcg
    14161 cagacagatt gctatcgcaa aagaaatgaa cctaccaatc agtattcata ctagagaagc
    14221 acttgaagac acgtatcaaa tcctaaaaga agaagatgtt cgagatatcg gtggaatcat
    14281 gcatagtttc agtggtgatt atgaatgggc aaaacgattt cttgacttag ggatgcatat
    14341 ttcatttagc ggtgtcgtca ctttcaaaaa agcattagac gtccaagagg ctgcagcaaa
    14401 tgtaccaatc gaacgattac ttgtggaaac agatgcccct tatctggcac cagttccttt
    14461 tagaggaaag cgaaatgaac caggctatac tcgttataca gtagaaaaga ttgctgaact
    14521 tcggaatctt ccgacagaag aagtggctgc ccaaacatgg acaaatgcgc accgattatt
    14581 tggtctaagc gaacatggat aagatgaaga tccaagagat tatagtcgtt gaaggaaaag
    14641 acgatacaag aaggctgcgg gaagtagttg atgcagatac gatagaaaca atagggtctg
    14701 caatcaatga agagatcctg atgcaaattg aacatgccca agagactcgt ggagtgatcg
    14761 tttttaccga tcctgatttt tcaggagaaa aaataagaaa aatcattatg gaagctgttc
    14821 ctcaggcaaa acatgctttt ttgccgcgca cacaagctaa accaaagaaa aagggaagcc
    14881 ttggtgtcga gcatgcaagt gatgctgcta ttttagaagc attgaaaaag gtcgtgacgc
    14941 cagacatttc ccaaacggct gtttctgaaa tcactagaca agacctttta gactatggtt
    15001 taatagctgg ggcacaagca aaagaaaaac gagaaaaact aggtgacgag ttgcgcatcg
    15061 gttacacgaa cggcaaacaa ttggaaaaac gtttgaagat gtttcgtatt acgccgaaag
    15121 aactcgccca agcaatgaaa atagtggagg aacaacatga gtgaatatcg tgaaattgcg
    15181 acaccctcta gaacaaaaga gattttattg aaacatggtt tttcatttaa aaaaagttta
    15241 ggacaaaatt ttctgactga accaaatatt ttaaggaaaa tcgtagaaac tgcgcaaata
    15301 gatgaacata cgaacgtgat cgaagttgga ccaggaatcg gtgcattgac ggaacaatta
    15361 gccaagcatg caaaacaagt cgtagcattt gagatcgatg atcgtttgat ccctgtattg
    15421 gctgatacga tgcaacccta tgacaatgtg actattgtac atcaagatat tttaaagacc
    15481 gatttgtcaa cagctgttag agaaacattt catgaggagc ttccgttaaa agtcgtagcg
    15541 aatttacctt actatattac gacaccgatc atgatgcatt ttcttgaatc agatttggtt
    15601 gtggatgaac tagtcgtcat gatgcaaaaa gaagtagctg atcgtatctc tgctgaacca
    15661 ggaacgaaag cttatggttc attatcgatt gctgttcaat attatatgga agccagttta
    15721 gcattcatcg taccaaaaac agtatttgtc cctcagccaa atgtagattc agcgattttg
    15781 aaactgacaa gaagagatat accggcagtc gaagtgacag acgaaaaagc atttttccga
    15841 ttgacaaaag cggcatttca gcaaagaaga aaaacgcttt ggaataactt gcagcattcg
    15901 tatggcaaag atgaccagac aaaagcatgg ttagcaaaaa gcttggaaac agctggaatc
    15961 gatccaaaac gtcgtgggga gactttgtca ttacaagaat tcgccgcatt aagcaatgcg
    16021 atgtcagaaa atatgcagta aaaaaagagg ttgtgacaga agttgtgaaa aagctgtcct
    16081 gcatcaagta ataagaacag ccaaacaaaa atagttcctc atgtttttcg tttgtttggg
    16141 cttattaccg cagatgcaag cttttgaaca ccgtttaaga aagaggttgt gggcctgccg
    16201 tttaactcta agtcacaagg aacaatttca aaaaacagct tctcatgttt ttgagttagt
    16261 tggtcttatt acttgatgca agcttttgac cactattatg ccgaacaagg agatgtgaca
    16321 caaattttgt cacatctcct atttttgttt cttgatcaaa aaattcttat acttgatact
    16381 cacactcatt ttgtatggat ttagaaaaga atatgtgtct tgacagttat tagtaagtga
    16441 gttatactaa gtcgacattt atgtgtcaag acatattagg agagagaaaa aatgagaaaa
    16501 aattcagttc gaaaactaac gatttctgct ttattgattg ggatgggggt cattatccca
    16561 atggtcatgc ctaaaatcat gattccacca gcttccttta ctttggcgtc tcatgtgcca
    16621 ttatttattg cgatgttttt tacaccagga gttgcagtgg ctgttgcgct aggtacgaca
    16681 tttggtttct ttttaacaac gccggttatc atcgcattgc gagcattgtc acatcttgtg
    16741 tttgcgttga ttggggcttg gtacttgcaa aagcatccga gtatcgtttt aaaaaatgga
    16801 cagtttactt ttgctaattg gcgtttccaa gggttcaatt ttgtgatcgg tgtgattcat
    16861 tctttagcag aaatgctagt agtcagcatc ttttacttta ttggtaatat gcctgaaaca
    16921 tattattctc aaggatattt ttatacagta ttcatcttga tggggattgg tgggcttatc
    16981 cacagtttag ttgattataa tattgcttat tttatagcag gaacactcag caaatatttt
    17041 gatatccccg tatttacggc agcaaaaaaa gaaaatcgga aagagtcgat tcaagggtac
    17101 gcgaagaaaa cgacatatta gacatgatct ctcaaaaaaa aaactctcat ctagtaatgg
    17161 ataataaatc ctctaagatg agagtttttt gtcctaaaaa gagctgcacc aacatcttct
    17221 ggcacagctc taacaggggg caatttatgg aagtttgatg acttgttctg tttctttatc
    17281 aaagaagtgg cctttgctga tgttgaaggc aaggtcgact atttcgcctg gtttatggaa
    17341 atctcttgca tctacttttg agatgaactc agtagaacca gtttttgtgt aaagcatcgt
    17401 ttcagctcca agtaattcgg aaacgacaac ttctgatttc actacggctt caggtgaagc
    17461 ttcaagggct acttgttcac tgtggatgtc ttctggacga ataccgaaga taacttcttt
    17521 tccttcataa cctttttcaa ctaatacttt gttttttcct tcaggaatac gaagcttaag
    17581 cccatgtcca tctgtgataa caccattttt caatgtcaca ttaaagaagt tcatggcagg
    17641 agatccaata aagccggcaa cgaacatatt gacgggtgta tcataaactt ctttcggtgt
    17701 accgatttgt tggataaatc cgtctttcat gataacgatt cggtcagcca tagtcattgc
    17761 ttctgtctga tcgtgagtaa catagatggt cgttgtttca agacgctggt gaagtttggc
    17821 aatctcagca cgcatggcta cacgtaattt agcatctaaa tttgataatg gctcatccat
    17881 taagaagact tttgcatccc gaacgattgc ccgtcccaat gccacacgtt ggcgttgtcc
    17941 accagataaa gcagctggtt ttcgtttcaa gtattctgtc agacctaaga tctctgctgc
    18001 attgtccaca cgttttttga tgtctgcttt atcatattta cgtaatttca agccaaaagc
    18061 catattatca aaaacagtca tatgaggata caatgcgtag ttttggaaaa ccatggcaat
    18121 atcacggtct ttaggtgcca cgtcattcat gacttttccg ccgatattta gttctccttc
    18181 agagatatct tccagtcccg cgatcattcg caaagtagtt gactttccac aaccggaagg
    18241 accaacaaaa acgataaatt cacggtcttt gattgataga ttaaaatcag ttacagaata
    18301 attttctgca ttgtcatatt ttttatatac gtttttcaga gccatttcta ccataatacg
    18361 ttccacactt tcttttttag ttttattaac tatttaaagt ataaatgaaa gcgttaacta
    18421 acttcaatgt catgttgaac aagaattttt tctttttttt gtcaatgtgc tagttttaag
    18481 ctttaataaa tatccataag aaataaaaac tgatacactg gagtaaataa gtatgttaac
    18541 aatatcaaaa ggagtgtctc ttatgaaaat cgctttgatc gctcatgacc gaaaaaaacc
    18601 tatgatggtg aaattagcaa cagcgtataa aacaatattg gaaaaacatg aactttttgc
    18661 taccgggacg actgggcaga ggatcacgga agcaaccggc ttgccgatcc atcggtttaa
    18721 atccggaccg ttaggcgggg atcagcaaat tggagccatg atttcggagg atcagttaga
    18781 tttggtgatt tttcttcgag acccgctggc tgcacagccc catgaaccag atgtaacagc
    18841 acttatccgt ttatctgatg tctatgagat tccgttagct acgaatatcg gaacagctga
    18901 ggtgctacta agaggactgg aagccggctt cctagatttt cgaagaatcg tccatgaaat
    18961 agacaagcat ccactttcat tttgatggat aagcgtatgg aaaaataacg tttaatgttc
    19021 gcctatatgt agctataaaa gctaaacaaa ctgataatat gcgatataat acgaacgtat
    19081 tggataatat aaacaataat attagttttt cgtatttgca gtaaatgtga aaatatgcta
    19141 tagtttctgt gggaaaagat cccaaaaata atacagagta gaaaataaag cgttaagtgc
    19201 taagaggatg ggatgttgcc tcttggacga aggacgtaat gttcgcggtt ttattttcgc
    19261 attcgctgca tgatacgatg tagcagctct taaaggagat gctaaaaatg aagaaacacc
    19321 ggttagacgc acgtatgatt gctattatgg gcttattgat tgcattgatg gtcactttgt
    19381 cccgactggt agcaatcgag accccattta ttaaaatcag tgtcacattt attccacaag
    19441 tgatcatggg cattcttttt ggtccttttt ggtcagggat aggcgcagtt ttagctgatt
    19501 tagtaggaat ggccctcttc tcgaaatctg cattctttat tggatttaca ttgaatgcat
    19561 ttattgaagg agcaatctac ggattctttt tctatcgaaa agagatcact tggaaaaatg
    19621 ccattctggc tacgcttagt gtcacgctga tcatcaactt atttttgaca ccattatggc
    19681 ttgcgttgat gtaccatgtg ccattgttca gctgggtcgt ttgggcgccc cgcctgttaa
    19741 aaacagtaat atggttaccg attcaatcaa ttgccattta ttacgtagga cgtagtattc
    19801 cttacaaaaa aattttaaga agtttagcga tccacgctaa ataaaaaaca agcagtcttc
    19861 ccatcccaga agactgcttg tttttattgt gctgtgaatt gtgcttgata gagccgtcgg
    19921 taataaccat ctttttgaag cagctcatca tgggtgccca tttcgacaat agctccctga
    19981 tccatcacta atatctggtc cgcatttttg attgtggaca ggcgatgggc aatcacaaaa
    20041 ctggtttttc cttccatcat tgctaagaag gcttgttgga tttttttctc tgtcaatgta
    20101 tcgactgagc tggtagcttc atctaagatc aacatagggg gattactgat catcgttcga
    20161 gcaatcgtca acagttggcg ttgtccatct gatatcttta acccttgacc gccaatctta
    20221 gtatctaatt tttccggcaa acgttctaca aattcataca tataagaggc ttttagggca
    20281 tcattgattt ctgcgtcact tgcttccgga ttcccaaaac gaagattttc tcgcaatgta
    20341 ctgtcaaata gccaagtatc ttgtaacacc attccaaaac ttttacgtaa tgtatcacgt
    20401 tggatagagc ggatgtcatg gtcatcgact gtaattgttc cttttgttgt gtcataaaaa
    20461 cgcatcagca aattaacaag tgtggatttt ccggcccctg tttttcctac tatagcaatg
    20521 gtttgacctg gataagcagt aaaattgaaa tcttcgatca aacgctgctt ggggtcataa
    20581 gaaaaagaaa catgatcaaa agagactttt cctttgatat ttttgggttc caaggcatag
    20641 gcatctggca aatcaggctt ttcttctggc tgatcgatca aatcaaatgc tcgttctaga
    20701 ccagaaaaag cggtttgcag ttgtgtcgtg attccagaaa gttcgataaa tggtttagaa
    20761 aactgacttg agtagatcgt aaagctggaa atcacaccaa cagtgattga cgaaccactc
    20821 ttcaatgcca ataggccgcc aactaaacca atcgataaat aagcaaggtg atctacaaaa
    20881 cgagaagacg ggtttgtcaa ggaagaagaa aattgtgccc gttgtccttt ttcatataga
    20941 tcagcattta gcagttcaaa ttgctgttga ctagcttctt cttgctgaaa agctttcacg
    21001 attttctggt taccgattcg ttcggtcaca aagcccgaga tatccccaat aatcctttgc
    21061 tgtctatcga aatttgtttg tgaagcacga gcaacgatcc aactcacaag gaaaataatt
    21121 ggtgtagaac aaagaacgac caatgtcaga agtggactta gacgcatcat aaaaataaac
    21181 gaaagaatga tgattgccac tccagaaaat aattgattga aggccgcaga aattgcaatg
    21241 gaaatattgt ccatatcatt tgtaaatcga ctgacgatat ttccgtggga gttttgatca
    21301 taataacgaa gtggcaatcg attcagatga gcaaatgcat cttttcttaa ttttgcaacg
    21361 gataagtaag ctaaccgatt gcttaacact tggatgagcc attgactgac tacgttgatt
    21421 aaaacgatac caccgaatag aataagtaaa tggaacagtc gattgaagcg gacctgtcct
    21481 tctccaatca tttgatcgat actttgacca atttgtaacg tcatcacaac tgttgcaatc
    21541 ccattgatga ttcctagtat cacagctagc caaatgtctt ttgcataggc agtaaggtaa
    21601 ggaatgaatc ggcggagcgt ctgcaaggat aattttttta ctttcataac tattcttcct
    21661 cctgtgatgc gacgatttcc tgatattctg ctgattgagc aagtaattct tcatgggtac
    21721 cctgagcagc aatcttacct tgatccatca ctaagatatg atgtgcttct tgaatagagc
    21781 ggacacgttg ggagataata atcactgtac tgccaagata agtctgtagc gccttacgca
    21841 aatccaagtc tgtttgataa tccaacgcac ttaaagagtc atctaaaatc agtaatgctg
    21901 gcttggcaat caaagctctt gcaatcgtca gtcgctgttt ttgtccgcca gagaaatttt
    21961 tcccattttc catgactttt gtatctaaac catttggaag ctgacggaca aattcttctg
    22021 cctgagcgat tttcagagct tcccaacatt ctttttctgt ggcgtctttc ttaccccatt
    22081 gaaggttttc acgaatagtc ccggtaaata gtacagaagt ttggggaaca agagcaattt
    22141 gctgacggag atgatccagc tgccaagaac gaacatccat tccgtccatt ataactgtac
    22201 cggaactgac atcatagaaa cgcggaatca attgtgtcaa agtcgttttc ccgcttccgg
    22261 ttggaccaat gattccaagg actgtatttc gcttcagctg aaatgtaata ttttggagag
    22321 aaagaccaga atcaggagta taacggaagt caacatggtc aaaagcgata attgcttcag
    22381 aagaaaaatc gggagtagta ctgacctcag cttcaagaat cgaattttct gtgtctagca
    22441 cctctttcac ccgatttcct gaagcttcgg cacgtgtgaa gatcacgacg aggtttgaaa
    22501 caacgatcag tgccaaaagc atttgattca tataattgat caaagcaaga acttgtcctt
    22561 gttgcaagct accaatattt actttgatcc cgccaactgt cagcaaacag atgatcccga
    22621 cattcatgat caaggttgta gcaggtgata atagggcaga gatattggaa acacgtaaat
    22681 aattgttcgc tagatcattt gtgcttttat cgaacttctc tgtttctttt tttgtctgcg
    22741 caaatgcgcg gatgacacga acaccgctaa gattctgact aacgtgttca ttgagctgat
    22801 ctagtttttc ttgcactttt tggtagagag gaacggtttt tttgatgata aaaaataaaa
    22861 tgattgagaa aataggcaat aatgctaaga aaaacagacc tacttcccag tcgatcacaa
    22921 aagccatgac cactgacccg atacttaaaa acggtgctct gatcaataaa cgaatgacca
    22981 ttgctaaagc tagctgaagc tgattgatgt cgtttgtcat tcgggtgatc aaggtatctg
    23041 ttccaaaatg attcaactcg ttgtgagaga aagtattgat ttttttgatt agctgattac
    23101 gtaactctgt accaaatcct tgtgaagcta ctgaagcata gtattggcaa atgatagcac
    23161 aaatcagacc aatcacagac atagcgacca tccataagcc catttcaatg atttttcctc
    23221 ggtcattcat ttttagtcca ttatcgatca aggaagccat cattaaaggc aggacaagtt
    23281 cgaagacagc ttcaagaaat ttgaagaacg ggcccaaaat gatttgctta cgatagtctt
    23341 ttgcataacg taataactga atcatatgat tcctcctatt atttatttgt ccacaattga
    23401 aaaaacaagt actaccacat tgtagcttag gaaatttaga atgaaaagag aacaagcctt
    23461 ttcataaatg aaagttttat tatgaagaag ctttagaaat tgaatagact atttttttta
    23521 aaaaaaagaa aatgagaaaa catgccagtc tcttcattca taggaacgtc tattttactt
    23581 aatttttcta taaatgaatt ctgcataaaa aatatatcaa gtaaatgttt atagatgaaa
    23641 ctctatttac ttctttttta atttgttctt gcaatctttt ttgctacgta atttttgtaa
    23701 aaaagtgggt tgagagaaat ctaattgatt ttcaaagaag gagtatgaaa atgagaaaaa
    23761 tatttattcg tttattgtgg gtactatctt agaggaataa ataatagtgg agaaaactat
    23821 ggtttggcgc agattctatc aggtttgatg gaacgaagac aacctcccga catcaagtca
    23881 aaagctactt gagattttga tctgaaaata aaaaaagaaa acaaggtagg aacatcaatg
    23941 tttctacctt gttttcgtaa tcaatgggaa aaaactaaga gtggtgtacg taatcaataa
    24001 aaaggatggt gacaacagta cctctttact ctctgaaagc atcataactc gtttttcctc
    24061 attatgccaa taaattgttg ttataccaca tataaggatt tgtaatagct aaaaacagac
    24121 aggaaagttg aaatctgtga atcttatttt gtatggaagg atttatagaa cgcaaaaatc
    24181 aagataaaga tgaataaaaa tcctgtttcg gctatatatt gcgtatctac gccaataaaa
    24241 cgagtttgtt ctgtcatagc catataaagc aaaggcaaaa atacaactac agcgagataa
    24301 gatccgatga tttctcgaaa agttttccag gaagtatgtt tcctgttttt atatttatcc
    24361 gatttcattg aatagtcata gcctccttat tctttcatat gttgaaattc cagtataaaa
    24421 actggaagga actgtacgtt ttttagaata aaaattataa gggataagta ggctgctgtc
    24481 tataataaga tatatttttc aattctttat taaagatttt attgacaaaa tgaatccgtt
    24541 ttctatgatg gaggaggggt gaataagttg ttgaagaagg aaattttatg ttacttagag
    24601 aaccagacag cctttttcga tttagaacat atgaacgagg tctttacagc acaaaggtta
    24661 gctgatcatt ttggggtaaa aagaaacaca gtcagtcatt atttgaacca gttaaatgat
    24721 gagcaactac tagtcaaaat caattctcga ccagtgattt atttccacaa agaagctttt
    24781 gaaaaacaat tttttaagtt acgcacgaat ttttattttt ctatcgatga tctcaaagag
    24841 gaacagcctt ttttcgcgca accaaaagac ttattctctt ttatgatcgg atacgacgcc
    24901 agtttgaaag aaagtatcga acaaatcaaa acagctcttt actatccaga tggcggatta
    24961 ccacttttga ttacaggcga aagtggcaca ggtaaaagtt atttagttaa tttggtctat
    25021 cagtattgtc tgcttcatga tctgctagaa gattctgcac cattcatcac ttttaattgt
    25081 gcacaatatg cagataatcc tgaacttttg actagcaatt tgtttggaca tgttaaggga
    25141 gcgtacactg gtgcagaaga aaataaaaaa ggggcttttg aagcagcaga tggtgggatc
    25201 ttgtttttag acgaggtaca tcgcttaaca ccagaaggtc aggaaaaact gttcacctat
    25261 cttgatcaag gtgtgattta tcgtatggga gaacctaatt tgtcacgccg gatcaaaaca
    25321 cgtctctttt ttgctacgac tgaagagatt actagtcatt tcttaacaac tttcattcga
    25381 agaataccta tccgaatcga actacctgca cttaatcagc gtagtcgtaa tgaacggcta
    25441 gaactgattt actctttttt tctcgaagaa caaagaaaaa tggggagccc tttgtcagtc
    25501 agtggaccag ctttgtcact tttaacaagt cagaaattat ccggaaacat aggagagctg
    25561 aaaaatatcg tcaaagtttc agcagcaaaa gcatatgctg aacagcgtga acagcctgaa
    25621 atccatgtca cgatccatca tttgcaaaaa gaactacttg cgcagcctat tactaaaaat
    25681 acagcacagc aaggaatcta tattacgcaa gaccagacct tagagcaatt aatggagaag
    25741 caacagcccg aacagcagcg aatcgttaaa agttttgagc aaatcctaat tgatttcaag
    25801 aaaaataact gtctgctata ttcttgcgaa ggaaaattaa agcaagaagt gattcaatta
    25861 tttgattttt tgctgtttga gaccagtaga caagaaaaac acgagctgct ggtttattgg
    25921 acacaatcta tccgagagac ctttcgacaa atggaaactg cctatcaaat caaattcgat
    25981 ggaaacagtg tctatgcctt gagttactat ctctatcagc gaagcactat caaatggtta
    26041 ccggaagaac atgatattat ccaactaatc aaagaattag aacaacaagt ttctcagtct
    26101 tatccatcta gttatcacta tgtccaacga attctggaat taagtaagat caaattagat
    26161 attgaagaca caagtatgga tcggatttta ctgacgatct atctaaaaaa agcgaaatgg
    26221 tcaaaagaaa aacgactgcc aaaagcgatg attgctgctc acggttatgc aactgctagc
    26281 agtatggcaa atgtagtaaa tcgtttatta caagaggatc tatttgaatc atttgatatg
    26341 ccactagatg tcacaccaca gcagattgct tcagaaatcc ttgattattg cgaaaacagc
    26401 gatgtttcaa atggattgat catattagta gacatgggat ctctgaaaga aatccatcag
    26461 ttttttaaaa aacagttatc cgtaccgctt ttgattttga ataatgtgac gacaccttta
    26521 gcaatcacag taggggaatg cctgcaaaaa aatgtatcat tagaagagat agcagaagag
    26581 actgttcgac agatccagcc agaatggcga ctgctctatc cagaggaaaa caaacccaaa
    26641 gcactcatta ctacttgctt cactggaatc ggtacggcgg tccacttaag tgaactgcta
    26701 gaaaaaagct tacctacggc ttgtcagttg aaaatcatcc catatgaata tcagcaatta
    26761 aaagataaga aaaacagtga cccattattt tccatatacg aagtcgtagg aatgattggc
    26821 accacagatc ctgatatcac caatatcgac tatctttcat tagaagacct catctcgggg
    26881 gaaaagatgt ctgtactagc tgaatggttg gacactacta tgaatgcttc agagaaggaa
    26941 atattcaatc agcgcatcat tcgtaatttt tcattggaaa aagtactgga ttccgtgact
    27001 attctagata cagagaaggt gatgggagaa atcgatcatt ttatgagaga attagaggtg
    27061 cagttgaatc gaagcatcag caatcccaaa aaattagcgt tgtatgtcca tgtgagctgt
    27121 ctcattgaac gattgatccg acaaatgcca atcgaaacct atcaaggatt agagaaatgg
    27181 aagcagtgtc aaaaagaagg gttatctgct attaaatctg cgtttagtgt cattgaagag
    27241 cattatagtg tcatgatccc caattctgag attgcctata tctatgatat tttgagtgaa
    27301 gatacagagt ttctctcgat agaagaagaa ttttgaacat aagcaagttg ttgccactaa
    27361 ttttggcatg caacttgctt ttttagtatg tgtaagtgaa aagaggagat gaaaaagtgg
    27421 atagagaaat tatcttagct tcccatggaa aatttgcttc agggatatta gattcattag
    27481 agctgatcta tgggaaaaat cattctatta ctgtattcga ttgctatact gatgagaatt
    27541 ttgatttagc ggaaacagta aggcagcttt ttatgaggta caaagataaa gaaatcattg
    27601 ttctgacgga tctttttggc ggaagtgtca acaacgaatt cttacaatat atcaatcggg
    27661 atcatgtcta tctcgttgcc ggattgaatt tgccactatt gattgagcta gtatctcggt
    27721 tggacgctga agtagagaca actgttttga tccagcaggt gctaactgat tcaaaaaaaa
    27781 tgatccagtt ttgtaacgag cggctcaatc agaaaataga agaagacgaa ttttaggagg
    27841 aagaaaaatg attgtattga caagagtcga ccatcggtta ttacacggac aagtagcttt
    27901 ttcatggaca caaacaatag gcgcagactg tattttgatt gctaatgatg atgtacctac
    27961 gaatgagata cgaaagacaa cgatcaaatt agccaagcca caaggagtca aactagtgat
    28021 caaatcaatt gatgactcga ttgccgcgtt gaaaagcggg gtgacagata aatataagtt
    28081 attcatcgta gtggaatcga tcgaggatgc atataagctg gcagaagcgt atccagaaat
    28141 caaaaccatc aacttaggag ggatcaaggc aaaacctggt actcgcagca tcggaaaagc
    28201 ggtaaatatt ttaccagaag aggaggaacg cttgaaacga ttaattgaca aacaaataga
    28261 agtagaaatc cgtcaagtac cagcagataa aaaagtggat gtaagagaag ttttgtaaga
    28321 aaggaagaac ctcaatgatt gtacaagcag tcttattagg aattgttgca tttgtcgcac
    28381 aatcagaata tgcactaggg acatcgttgc tttctcgtcc gattgtgacg gggctgttta
    28441 caggaatcgt gttaggtgat gtaaaaactg ggatcattat gggcgcaact ttagagttag
    28501 catttatcgg atcgttctca gtaggtgctt cgattccacc tgatgttgtg accggtggga
    28561 ttttagggac ggcttttgct attacagctg gagccggtac agaaaccgcg cttttactag
    28621 gtttgccgat tgctacgttg acattgatat tgaaaaatat ttacttaggg cttttgattc
    28681 caattatgaa ccataaagca gacaattatg cagaagaagg aaattataaa ggagtcgagc
    28741 atatgcactt gcttgccggt atcggtcttt ccttgatgct tggtgttgtg gtaatggttt
    28801 cttatatggt gggaagcaat acgatcggaa atctattaaa tatgattcct gacttcgttc
    28861 aaaaggggtt gtcagtagct acagggttga ttccagctct tggttttgcg atgcttgctc
    28921 gtttgctgat caacaaacaa gttgcaccat attttttctt aggttttgct atagcttctt
    28981 acttagagat tcctgtgacc ggagtagcta tttttggtgc gattttagca gttgtggtag
    29041 tcaatattat gaatgtccgt caactaccac ttcaaacaac tgcaggggag gtagaagaag
    29101 atgaagaatt ctgagatcaa aaacagcaaa ataacgaaaa aagagctaaa tcacgtattt
    29161 tggcgttctt ttcaaatgga gttttcttgg aattatgaac gtcagatgaa tatggcttat
    29221 gtttatgcaa tgattccgat tttgaaaaag ttatatcagt cgaaagaaga aatggcagca
    29281 gctttgaaac ggcatctaga gttttttaac acgaccccac atatcgttac actgatttta
    29341 ggaatcacgg ctgcaatgga ggaagaaaat aaggatgatc ctgaatttga tgtgactgcg
    29401 atcgatagta tcaaaacatc tttgatggga cctcttgcag ggttaggaga ttctttcttt
    29461 tggggaactt tgcgattgat tgccacaggt gtgggtacct ctttagccat gcaaggaaat
    29521 attttaggcc ctatcttgtt cattttgatt ttcaatattc cgcacattct gtttcgctat
    29581 ctagcaacag gttggggata tcgcttagga acgggttttt tgaaaaagat ccaggcaaac
    29641 ggtatgatgg aaagtttgac attaggcgcg tctattattg gactgatggt cgttggagca
    29701 atgactgcca cgatgatcac catcaatatt cctttaaaaa tcggttcagg agaaaatgct
    29761 gtgactgttc aaagtatttt tgatgatatc gttccaaata tcttatgtct tggagcattt
    29821 ggcatagtgt tttatctatt gaagaaagaa gtgaaaccat taacgatctt gatcggacta
    29881 gctttctttg gaatttttgg ttcgtggata gggatttttt aaaaaaggag cagagaaaat
    29941 gtttacgatg caagattata tctatgaaga aaaggaagta ctaagtacga tcttgaaaaa
    30001 gaatgatttt tcgacaagag aacatatgaa aaaaacagtg aatttactga tcttagcaac
    30061 gggttcatct tataacgctt gtttagcagc gaagccagca ttggaatctt atggtgatct
    30121 tactgtcgat attcaagaac ctttttattt tttcaattat ggaaaacttt ctccttcaat
    30181 cgatactgtg attgctgtgt cacaaagcgg gaaaagtgct tctactgttg aagcagtaaa
    30241 aatgatacaa aaacaagggt tacctgttgt tgctatcaca aatgacgtgc aaagtccact
    30301 tgctttagaa gctgcacaaa ttattgattt aggtgccggg gtagaatcag taggcttcgt
    30361 tacaaaaggc tattctgcga ctgttttaca gttactactc ttagggatag gaattggaat
    30421 aagtaaaaat aaaatcagca aaaaaataga acaagactac atgcaacagt tgcgcaaaat
    30481 aatcaaccat ttaccagcga ttattcaaaa aacggaagaa ttttttgatg gatatcaaag
    30541 tttatttcgt ttagcacagc gtttcgtcac cattggatat ggtccaaact ggggaacagc
    30601 taaagaagca gagacgaaat tgactgaaac aatccgcgtt ccgtcacaag gatttgagtt
    30661 agaagcttat atgcacggcc catacctgga ggcagatgct tcgcatttgc tattctttat
    30721 tgaaggggaa agtgtaaata aggaacgttc gcaaaagttg caaagatata tgagtcggta
    30781 cgttggagag actctcacca ttaccacaaa aaaagcaaga aacgagaaaa cacttggatt
    30841 agcaatagaa tgtgatgaat atctgtctgt attagcgctt gtcgttccgt ttcaactatt
    30901 cgcttataaa accgcagtag ctaaaggtat tgacttgaac aaaaagattt ttgaagactt
    30961 tgatacagtc ttgaaaagta aattataaga ggtggaaatc atgctgaaat tcaatgaaga
    31021 agaacaaatc aaagcaataa aaggcgcact agctttacgt ccgcaagtag aagaaatcat
    31081 cgataaactt tatgcggaaa aatttgacgc tgtctattat ctaggaatcg gtggaacgta
    31141 tgcttcctcg atgcaagctg tcacttatat gaatgggaaa agcaacttgc ctgtctttgt
    31201 acagcatgct gcagaatatt acactactgg aaacaaaaga ttaacaaaag attctatcgt
    31261 tattttgtcc tctgttaccg gtacaactca agaagtagtg aaagcagtag aacaaatcaa
    31321 gaaagtagga gcgacactga ttggatttat cgataaagcg aacagtaagt tgtctcagct
    31381 atgcgatttt gtggtcactt atccagctcc tggaacagaa caaatcaaat tttttatggc
    31441 agcagatcga ttgatgtatt tgaatggaga attcgaagct tattcagaat actatgaaca
    31501 gctcgaacgt tatttaccaa cagggttagt ggaagcagag aaaaaagcag atgcatttgg
    31561 cttatcattt gcagaaaaac atcgacgtga tagtatgcat tattttattg gagcagggaa
    31621 tcaatgggga gctgtttatt catatgccat gtgttactgg gaagaacaaa gctggcttcc
    31681 atcgaaatct atccatgctg ccgagtttct tcacgggacg ttagagatcg tagaagaaac
    31741 aaccccagtc acattatttt taggcgaaga tgaacagaga gtgttatctg aacgtgtggc
    31801 taaacttttg ccaaaaattt gcagcaatta tacattgata gacacaaaag actatccagt
    31861 agaaggtatc agtgaaaaat atcgcggacg tgttttgtct ttcttattga tgcatgtagt
    31921 gacacaacgc atcgatgctc atgtggagca gttgaactgc catccattag agatacgccg
    31981 ctactatcgt cagtttgatt attaggatag actggataaa aagaatataa aaaataaaga
    32041 gagcagcgat aactgctccc tttatttttt taaaatattg gcatcaatta cgttataaaa
    32101 cacatttttt gcattatcta cagttcaact tataatctgt ttaatgggac atgtgggtgc
    32161 caaccatttt ttgttatgca atagtactat ttagttgttg catggatccg tagtaaaagt
    32221 ccaagtacaa cgagttgcgc cagtttacat agcgttttgt atccagaatg ttgactaaaa
    32281 caaaagttcc tatgtcaacc gtcaacagag acaatctgtc cgttgcagga cctgacagat
    32341 ttagaaatgt tcaatctgtc tcttatgtat tttaaatcaa tactcactag gagaagtttc
    32401 ctaacctcct aatagtaatg ctggtagtaa cattgtcaaa actgcttcct cttaggaaca
    32461 agcaaagcat aacgagtgta aaaataaaat gtagactatt gtaacatatc attgattatt
    32521 tcaagtaaaa acgataaaag aagtaaacaa acacttaaaa acaatggaaa ttagtcccaa
    32581 ctttaagaaa tgtatactat aatggaaatt atcaattatt tatttggagg aatatccatg
    32641 aaacaaaaaa tcgcagttct tggtcctgga tcttggggca ctgctttagc acaaacattg
    32701 gcagaaaacg ggcatgatgt ccgtatttgg ggaaatgtac ctgaacagat tgatgaaatc
    32761 aataagtatc atacaaatca gcatttttta cctgatttga cgatcccaga atcgattatc
    32821 ggatataaag aattggcaga agcagtggat aacgcagatg ctattttatt tgttgttcca
    32881 acaaaagcga ttcgttcagt cgcacaggaa cttattggta aaatgaatac taagccaatc
    32941 attatacatg caagtaaagg actagaacaa gatacgcata aacgaatctc agaagttttg
    33001 gctgaagaaa ttccagaaga aaaaagacag gcaattgttg tattatctgg tcctagccat
    33061 gctgaggaag tggcagtaca tgatatcaca acaattacag ctgcaagtat tgatcaaaaa
    33121 gcagcagcct atgtacaaaa actgttcatg aatgattatt tcaggattta tacaaataat
    33181 gatgtgattg gcgtagaaac tggtgcagca ctgaaaaata tcattgcaat tggtgccggc
    33241 gctatccatg gattaggatt cggagatgat gcaaaagcag caatcatgac tcgaggattg
    33301 gcagaaatca gccgtcttgg tgtcgcgatg ggtgccaatc ctctgacatt tatcggtttg
    33361 agtggtgtgg gggacttgat cgttacttgt acaagtgttc attctcgcaa ttggcgtgcc
    33421 ggtaacttgt taggacaggg acataagctg gaagaagttc tagaaaacat gggaatggtc
    33481 gttgagggtg tggcaacgac aaaagcagca gtagagctag ctcaacagct aggtgttgag
    33541 atgccaatta ctcaaacaat ctataatgtt ttgtataatg gagaagatat caaaaaagca
    33601 gcaaaagaaa tcatgttacg tgacggaaaa atggagaatg aattttctct tcgttaaata
    33661 gatgatcaat agttaatc
//
LOCUS       DAYYGS010000024        33677 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000024 DAYYGS010000000
VERSION     DAYYGS010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.024"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            214..498
                     /locus_tag="ACHA44_001054"
     CDS             214..498
                     /locus_tag="ACHA44_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HFM8904363.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(815..1198)
                     /locus_tag="ACHA44_001055"
     CDS             complement(815..1198)
                     /locus_tag="ACHA44_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HFM8904364.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIK"
     gene            complement(1200..1784)
                     /locus_tag="ACHA44_001056"
     CDS             complement(1200..1784)
                     /locus_tag="ACHA44_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFM8904365.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="ACHA44_001057"
     CDS             complement(1831..3306)
                     /locus_tag="ACHA44_001057"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HFM8904366.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="ACHA44_001058"
     CDS             3487..3798
                     /locus_tag="ACHA44_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904367.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="ACHA44_001059"
     CDS             complement(3913..6249)
                     /locus_tag="ACHA44_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8904368.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDRKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="ACHA44_001060"
     CDS             6391..7059
                     /locus_tag="ACHA44_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8904369.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="ACHA44_001061"
     CDS             7062..8105
                     /locus_tag="ACHA44_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8904370.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="ACHA44_001062"
     CDS             complement(8342..9223)
                     /locus_tag="ACHA44_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8904371.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="ACHA44_001063"
     CDS             9382..10110
                     /locus_tag="ACHA44_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFM8904372.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="ACHA44_001064"
     CDS             10266..10583
                     /locus_tag="ACHA44_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8904373.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="ACHA44_001065"
     CDS             10576..11184
                     /locus_tag="ACHA44_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HFM8904374.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="ACHA44_001066"
     CDS             11239..12030
                     /locus_tag="ACHA44_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HFM8904375.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12656
                     /locus_tag="ACHA44_001067"
     CDS             12168..12656
                     /locus_tag="ACHA44_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HFM8904376.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12707..13579
                     /locus_tag="ACHA44_001068"
     CDS             12707..13579
                     /locus_tag="ACHA44_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFM8904377.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13755..14117
                     /locus_tag="ACHA44_001069"
     CDS             13755..14117
                     /locus_tag="ACHA44_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8904378.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14126..14878
                     /locus_tag="ACHA44_001070"
     CDS             14126..14878
                     /locus_tag="ACHA44_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904379.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14892..16694
                     /locus_tag="ACHA44_001071"
     CDS             14892..16694
                     /locus_tag="ACHA44_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904380.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16971..18011
                     /locus_tag="ACHA44_001072"
     CDS             16971..18011
                     /locus_tag="ACHA44_001072"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8904381.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18063..20207)
                     /locus_tag="ACHA44_001073"
     CDS             complement(18063..20207)
                     /locus_tag="ACHA44_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8904382.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLRLDKSFWLDLPLKK"
     gene            complement(20564..21937)
                     /locus_tag="ACHA44_001074"
     CDS             complement(20564..21937)
                     /locus_tag="ACHA44_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8904383.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHA44_001075"
     CDS             complement(21937..22332)
                     /gene="rbsD"
                     /locus_tag="ACHA44_001075"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFM8904384.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHA44_001076"
     CDS             complement(22360..23277)
                     /gene="rbsK"
                     /locus_tag="ACHA44_001076"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFM8904385.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23385..24398)
                     /locus_tag="ACHA44_001077"
     CDS             complement(23385..24398)
                     /locus_tag="ACHA44_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFM8904386.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24479..25078)
                     /locus_tag="ACHA44_001078"
     CDS             complement(24479..25078)
                     /locus_tag="ACHA44_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8904387.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25264..25965
                     /locus_tag="ACHA44_001079"
     CDS             25264..25965
                     /locus_tag="ACHA44_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904388.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25975..28686
                     /locus_tag="ACHA44_001080"
     CDS             25975..28686
                     /locus_tag="ACHA44_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HFM8904389.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29137..30765
                     /gene="treC"
                     /locus_tag="ACHA44_001081"
     CDS             29137..30765
                     /gene="treC"
                     /locus_tag="ACHA44_001081"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFM8904390.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30825..32006)
                     /locus_tag="ACHA44_001082"
     CDS             complement(30825..32006)
                     /locus_tag="ACHA44_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8904391.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32258..>33677
                     /locus_tag="ACHA44_001083"
     CDS             32258..>33677
                     /locus_tag="ACHA44_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HFM8904392.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAG"
BASE COUNT        10661 a         6038 c         6850 g        10128 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttattt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcattg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gtttttctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa aattaaaaaa
    12661 gtaacattag atatttattg aaggataaag gagcgcagga agtagcatga gagaaaaaag
    12721 agcattttat atcaatggtt atgcagggat tgtcggattg gtcgtattag ctttagttgg
    12781 attgtttttc ttctatttag gtatgtggca agcgaaagtt tgggcacttt ttttaagtat
    12841 ttttctttgg ttgatcacgt tattgttgtt aagttcggca actgtagtca gtcctaatca
    12901 agcaaaagta attttgtttt ttggacaata tttaggtacg attcgagaaa atggcttttt
    12961 ccttacgata cctttggcac aaaaaatgac tgtttcgttg aaagttcgga atttcaatag
    13021 ttctgtttta aaagtcaatg atttggatgg aaaccccatc gaaatctcag cagtagtagt
    13081 ctttaaagtc gttgatacag caaaggcatt attcgatgta gcgtattatc aagattttgt
    13141 agaaatccaa agtgagacag ccattcggca tatcgctagc caatacccgt atgatacatt
    13201 caatgagaat gatttgacgc ttcgaggaaa cacgacggca gtgtctgacg agttgcaaaa
    13261 agagctgcaa gagcgtttag cagtagcagg tgtcgaagta atcgaaacac gcttgaacca
    13321 tttagcctat gcaacagaaa tcgctagtgc catgctccaa cgtcaacaag caaaagccat
    13381 tttgtctgct cgacaaacca ttgttgaagg agctgtcaca atcactcaaa tggccttaga
    13441 gcaaatcgaa gatggacaag aaatcaattt caccgatgat cggaaagtcc aattgatcaa
    13501 taatttactt gtctcaatca ttacagacaa aggaacgcaa cctgttatca atacaggcga
    13561 catgaaagac cgtaagtgac atactttttg taagatagga agaaggagtt taggacggat
    13621 tattgtcttg aactcttttt ttagttgttt taaagaaaat gacaaaattg aaagaagaat
    13681 gatagttgat tcgaaggcag aattgttcgc ttttttctat aatcagtata taaagtaaga
    13741 gagaagggga aaaagtgaaa aaacttctga tcggtctact tgttttcatc gttgtggtac
    13801 ttgcaggggt gaaaatcgca gattacgtag ttatgggtgg agaagaatac tatgtacaaa
    13861 ttacgacaga tggtacaaaa acagtttcaa aatcagactc tggacaagaa acagtcaact
    13921 atagatatat cctgcctggc tatacgaaag agggtaagga aaaagaactg gacttcaagg
    13981 gacaattgac tcgaccgctt cgtaaaggag catatttaaa aatcacctgg aacaaaaata
    14041 aaggtgtcac ttcgtatgaa gaagtcgaaa aaaaagacgt gccgaaaaca gcactggaaa
    14101 aactaattaa agggtaggga aagaaatgaa caaaatagtc gaagtacaga atgtaacaaa
    14161 agtatacgga aaaaacaatg agaaaaagac gcaagcatta agcggcatca gcttcgatgt
    14221 agaaaaaggc gaatttattg ggatcatggg cgcttctggt tctggtaagt cgacactttt
    14281 gaatatttta tccacattag ataaaccaac agatgggcat atccgaatca atcaaaaaga
    14341 tgtgaccaca ttaaaaggaa atcaactagc agattttcgt gcgaatgaga ttggctttat
    14401 tttccaagat ttcaatttat tagaaagctt gacagcacaa gaaaatatcg ctgttcctct
    14461 ttctttacaa ggcgtcagac caaaagaaat caagcaacgt gtccaaaaaa ttgcagaacg
    14521 tttatccatc tctcatattt tggaaagcta tccttctgag atttccggtg gacaaaaaca
    14581 acgggtcgcc gccgcacgtg cattgatcac acaaccaacg attctattag gggatgagcc
    14641 tacaggtgcg ctggattcca aaagtgcccg ggatctttta gatacgatgg acgaattgaa
    14701 tacaaaagat catgtatcga ttcttcttgt gacgcatgat ccgttttctg caagttattg
    14761 ccaacgtatt ttattcatca aggatggcgg tatccatcaa gaagtgaaac gtggagatca
    14821 aagccgtgat tccttctatc gagaaatact atctattctt ggaaacttgg aacagtaggg
    14881 ggagattgac tatgctttgg aaattatcac ttactgggat caagagccgt ttaagagact
    14941 acttggtgtt attctcagga ttagttatgg catctgctat tttttacatg tttgaatcaa
    15001 tggcaagcaa cgaagctttt ttgaaaagta atacgatcgt tgcggctaca gttatcattt
    15061 tccgattcgg ttctgtttta ctagggatca tcacattcgt ttatatttta tatgcaaatt
    15121 cctttttgat gacgatgcgg caaaaagact atgcaatgtt tatgatgctg ggagcaaaag
    15181 gcagaaaaat cgcacagatg attttttctg aaacattctt agtagggatc gcagctactt
    15241 tagtgggttc agctgtcggt gtaggattga caagtatcgt ccaccgatta ttagtggatc
    15301 agttagatct caatatttcg cattttacac cttttagtat tcacgggttg ttgatcactg
    15361 ttttgttttt tgctgttttg tttttattag ctgctgttgt caatgctttt tcgattgtca
    15421 aaaaaccgat tttagcattg attcgtgcgg atcagacacc aacaagaatc aagcagcata
    15481 aattcttgtt tttccttgaa gtagtactgg gaattgcttt tctaggctgc ggttactata
    15541 tgatgaaaca cgtggggacc tatcaagtct ttggtatagg ggtagcctta gttacgatcg
    15601 tattaggaac ttatttcatt ttccattctg tgatcatttt ctttttatca ttactaaaga
    15661 aatcagaaca gatttcattg aaaaaattga acaatttcac attgtcacag ttaaactttc
    15721 gtattcgtga atatacacaa atgctctcaa tggtcgctat gctgtttgcc ttggcactgg
    15781 gcgctttgac ggtaggatta ggttttaaaa atgaaattgc agacttgaca gaaaacaatt
    15841 ctggatatga tctagtgatg aataatcctc gggcagaaga tcagcaaaaa atcaaagaac
    15901 tttcaccaac actttctgct tcttatactc aaaaagaaga tgcgggaacg atttactata
    15961 atgcaagtga atttgcggct caaccattga tcatgataaa acatgaagat ggaacaccgc
    16021 cgaagatcac gaaagaaaaa gttcctgcta aaaaaatgaa tgaactgact gtacaagagg
    16081 aacttcgaag ctatgaattg ccggaacagc aagaaaaaga aatcaaggta gtcagtcaat
    16141 cagaatttga tcaactgaac ttgccacaat ccactttaca gctagttcag gtaaaagact
    16201 tccaagcaaa cctgaaacca atcgaaacac ttgttgctca aaataaaatg aacaatccat
    16261 ctttgcaaaa agtagagtac agcaaccaga aatatgaaat gtatgaagtg tataatggtc
    16321 tattctctgg ttttgaattt atgggattct tcctaggttt agctttcttg accatgcttg
    16381 ctagctgttt gatgttcaag atcttgtcag gaagcaagag tgacattgct cgttatgaaa
    16441 tgcttgaaaa gataggaaca cgacgcgggt tgcttaaaca atcgatccgt agagaagtag
    16501 gcgtgttatt cttagcacca ggaatcttag gtgtagtcca tgtcttattc ggattacaaa
    16561 tgtttggtct attcatgcaa aatccataca aagatatttg ggttccgttt acgatattct
    16621 tcgtattgta tttcatctac tatgtactga cgacatggtt atatactgga atcgtattga
    16681 aaaagagatt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa gcaaaaatag
    16741 ctcctcatat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt tgaacaccgt
    16801 ttgttcggaa aaagaggttg tgacaatatt tttgtcacaa cctttttttc acggtctttt
    16861 attagaatat agtaaatttt ttctcgttac tcacggaata agctgttaag aaaacggttt
    16921 ccatgtatac tggaatcagt gcaaatgaaa gaaaagaggg tgcaaggaac atggaaatca
    16981 atgaacaaaa ttttggcgaa aactatcact tgattacgat cacgaatcaa aatgggacat
    17041 ctttgtctgt cagtgatctt ggggcacggg ttgttcattt tcaaacaata atcgataatg
    17101 aaaaacgaga gttgatctta ggttttgatg aagcaaaaga atatctagaa aaagaccctt
    17161 atatcggtgc ttcaatcgga cgtatggctg gacggattac gaatggcaca ttcaaattag
    17221 atgatgaaac gttcgaagta aaaacagacc cggacactgg tcattgttta catggtggtg
    17281 cacctagctt tgaagccaag cagtggcatt atacagtcat caatggtgaa aacgaagcat
    17341 cggtcatttt ctatcagact agcccagata tggaaaacgg atttcctgga actttagatg
    17401 tagaagtacg gtatacgctg acgaacgatg atacatggcg agtgacggtc caagggatca
    17461 gtgaccgcaa aacagtattc aatcctacaa atcatgtcta tttcaattta accggtgatc
    17521 cagcacagtc aatcgacgaa catatacttt ggttgaatag ccgattattt gctacgctca
    17581 ataaagatac aacgcctaca ggagaagtag tttcagtaag tggcacagca tttgacttcc
    17641 aacgtcctaa aaaattaggt gaggtctttg ccagtgattt tcgccaatca atgatagtgg
    17701 gcggcatcga tcatccattt ttgttgaaaa aacaatctgg tttactaaat gctgcggaat
    17761 tgacaagccc agatgaaaaa gtatcgatcc aagtaaaaac agatgcttca ggaatcgtga
    17821 tttttacagc aaattttgga gaagatggac cggaaatgcg cgggaataaa ttagtaaatc
    17881 atggcggtat cacatttgag actcaagaac tgccaggagc ggagcgactt gaaagttttg
    17941 gagatgtcac tctggaacct aaccaagtac gcgagtttgt aacggaatac aaaataaata
    18001 taaagaaata gaataaatta aaaagggagt gtgacagtgt cacatcccct ttttaattta
    18061 ttctatttct ttaaaggcag atcaagccag aatgatttgt ctaaccttaa caaggttagt
    18121 acaggaacag ttgcagcttt aactgtacga gtagttgttt ctggtatctg tcttgccgta
    18181 aaattgttcc acaaatccca gattgaagcg acacggaaga tggaagaagc gttgttgacg
    18241 cgttttatcg ttgaaaattc taatcctgtg attggcacgc catctgttgt ccatttgatt
    18301 ggcacatcga atcgcttaga catattgagg atttcagcgt ttgcttcttc agttgttgct
    18361 gcttcttttt tcggttcatc atattttgtc aggtcgtacg cttcgatgat cgcgttcaat
    18421 ttttgtgaat attggcgatc tgtagcataa cgtccttcca aatatttggt tgcttctttg
    18481 tagtcttttg tttccgactt ccaagttggt ttgtagaaat caggattgcc agtcagtcca
    18541 tttttgatca agtcggcata gtcttccaaa ctttccttgt aagaaggata tttacggaat
    18601 tgtgcgcgga tttggtagct ttgtccggaa ctatcttgtt caagtgtatt gaaggtcacg
    18661 ctgcttcctt tatatgatcc tttgatacca aaaaggttat gatttggttc gcttgataat
    18721 aagctgtttc cagatccact ttcaaggata gcttgagcca tcatgactga tgcgtagatt
    18781 ccttcttttt gcccgatttc ctgtgcatca tctgcaatct ttttcaagaa ctgatcagtt
    18841 gtttggtttt gactgaaatg gaacgagcca tctaatccat ctaaaataga tgagccgcct
    18901 gtcagcgaac ctgaagcagc aggtaaacct gctaagttta ctcggacagc aaaatcaggc
    18961 gtgaaatctg aaatagaagt tactcgaact ttatctcctg gttgaggcgc atggataaat
    19021 tgtccgttcc caatatagat acctacatgg tgagtaacac cggaagaagg tccccagaag
    19081 acgaggtcgc caggttgggc ctgacttact ggaatctgcg tacctgcata ttgctgttcg
    19141 cctgtccagc caccgatata atgaccggtt gctttcatat atacgtaata agtcaaacca
    19201 gaacagtcaa aagtatttgg accttttgct ccccatacat atggttttcc aagatgcttg
    19261 aatgcttctc gaacgattgc ttgttggaca ttcgttgtat ttgtaggcaa tacagcatca
    19321 attggtgatt ttacaacaaa atcagaagaa gcagaagatg gattctctat accgctttga
    19381 tttgctgtat ttggacgaga tgaagcattg ttcgttccgc cattcgatga accattgttt
    19441 ggatggtttg gttctctctg agtatttcca ttgttattcg atccagatgg gtttgtttga
    19501 ttcgaagaat tcccgctgtt cgaattattg ttattattgt ttgaattatt tgaaccatta
    19561 ttattgttgt tattacccac atctggtttg tttggcgttt ccggatctgg tgtactaggg
    19621 acttcttcac taggatcagt tggtttctct gttgaagtat ccggtccagc tttaaattct
    19681 gcagtaatag ataaattgtt cgtacctact gtaaaggtct ttgcatcata atttacttct
    19741 ttggcaccat taacagcgac tgaagcaatt tgttcacctt cagctggcgt aatagtgtaa
    19801 gttaccttcg tttccgctaa taaaccttct accgttccac tttccgtaat atccaaaggt
    19861 tgctgttcag gggaatccgc tgtcgaagca gtcagttgga tcttaccaat caaagaagga
    19921 gcaattgtaa gggaatatgt ctcggctggc ttttcatttt ctgatggatc agccggatca
    19981 atttcagttg gctcggtcga tcctgttgtt gagttagttg ttccagttgg gtcttctgta
    20041 aaagaatccg ttgtttcact agtagctgag ctggatgtag tcgtatctgt tgtgactgtt
    20101 tgatcagcta tttgattatc tacggcaaat acggtattgg tcgacaaaac agacggagca
    20161 atcagccaag tagcagctag caaacgtatc gtttttttat ttttcataat aaactagtcc
    20221 ttttcctttc attgattttc tgtcttattc agcattcatt agacattttt tggaacagat
    20281 aatcgtgtcg aaaagctttt tcttttcata aatctgtctg tgtattctaa ataaaacaat
    20341 ttgtaaataa gcaacgagca ttcattcgtt tttctttgac gttcatttta ttgcatagac
    20401 cacctttgtt tggggataaa caagtgggga taaaaatggt tatgcatatg taaattatac
    20461 caaagtttgg aaagtatcac aaaaatttat aaaaataatg atcctaaaaa gaaaaagact
    20521 gaaatatgtg ataaacatct catacttcag tctttttcat taactattca taagacatta
    20581 ggtcttcatc aaacccgcta acgagagtag tgatcattgc tacgactaga ctaacagcca
    20641 gtcctgctaa gtaccagaaa aaagtaccgg aaatgaatgc aggcaatgtg gtcagactac
    20701 caaatacaaa agcgatcgct ttcacttggg caaatcccat tacagctcca ccagcagctg
    20761 caccaatgca agcagcaaca aatggttttt tcaatgggat cgcaattccg taaacagttg
    20821 gttcggtcac tcccgcaagt aaaatcgaag caaaagcaga accggagatc gaacgggttt
    20881 ttgaacgttt acttcgcaga aatacaccta aagctgcacc agccattcca aaattggctg
    20941 ctcctgtcaa cggaacgatt cggtcgaacc cataagcaga gatattactg atcatgacag
    21001 ggtttacagc ccattggata ccaaatatta ccaaaatatt ccaaccacca gcgatgattg
    21061 cgccactgat cggtccgtta atatccatca tccaagtaat tgcggcagct aaaccttcgc
    21121 caaggtaaac acccgcaggc ccgaatacca ttaacgtcaa aggaaccgtg ataactaatg
    21181 aaagtaaagg tacaaatact aattgcatat tttcgcgaat atattttttg aggaattttt
    21241 caacaaaaga ataaaagaaa atacctaaaa tcgccggcat gacagtagac gtatactgca
    21301 tcagtactac aggaatcccc agaaaatcag tagctgcttt gccactgtct gaaagtaaag
    21361 aggtcagatt cggttcaatc agtgctgcag caatgacagc cgcaatatat ccattcactt
    21421 taaacgtttt ggcagcagaa aaagcaagaa agattggcag aaaataaaag acggcattcg
    21481 acgcagcaga caaaatatga taagttccgc ttgtttcact aagccagcca atcgtagtcg
    21541 ctaaaacaac cagacctttt aatataccag aaccggcaaa cagcggaatg atcggcgtaa
    21601 agatttccga tatcaaacca aatagttttt cgatcggatt tagcttttct ttcggagctg
    21661 aatgatcgat ttgacgcgtc aattccttgt aagcaggagc caatccctga tccatgatta
    21721 tttctgcata tctatcgagt aaaatgaaat acttaatgga agggatttgc tgttttaatt
    21781 tttctgcatt ggctactggc tgttttttca atttgatacg aagccgagtc gcacatggaa
    21841 ggacagaatc aatattgtct tttcctccca actgttcaat cagcatatga atctgctcgg
    21901 acagtaccat ctgttcagga ttcattgtcg tttccattag aagaccactc cactcactaa
    21961 tataatattg gcgtaaggtg tcatttcgcc tgtacgaata aatgcatgac agttattcaa
    22021 ctcttttttc atttcttcat gtgggataaa gctaatcggt gtatctggta acaataattt
    22081 gattttttcc aatatttccg ggttcattgt ctttatttct tccgccaatt caatcgattg
    22141 tacctcaagt tcttctaaaa cgttttctaa aacagataaa aaacttggaa gaccgtttga
    22201 gacagccaaa tctatttttt cagtaccaaa ggggacaggc atgcctgcat ccccgatact
    22261 taatttatca aagtgcccca tttgtgcgat tactcgtgaa atatctgaat tgattacttt
    22321 tccttttttc atgtttgttt cttcctctca ttgttaattt tagactgctg attgttctaa
    22381 agcttcgcgg taagggatag aaggttgcgc accataagac tgcacagcaa cagaagaggc
    22441 tagatttcca taaaggatcg cttctttcaa gttactaagg tctggctgaa gtgtgctgct
    22501 taatgcgcca ataaatgtat cacctgcggc tgttgtgtca acggcatcca ctttaaaagc
    22561 agggacgata ccgtgttctt tttctgtatg atagaaagcg ccggcactgc ctagtgtaat
    22621 gattactgtt ccaataccta attcatggag tttttcggct gctgcgacca aactgttatg
    22681 atctgtcact ctgattcctg taatgatttc tgtttctgtt tcatttggta taatgatatc
    22741 tgtcttttca agtagttcag taggaatcgt ttcacgagct ggtgcaggat tcaggatcgt
    22801 tgtttttcct gcgtcttttg caatagaaaa agctaggatc gtcgcatcta agctactttc
    22861 aaactgagca ataataaagt cactgtttgt aatcagttgc ttgtttttta ggacttcttg
    22921 ttcatggaaa gcattatttg cccctgcatg gatcaagata ctattttctc ctgctgcgtc
    22981 taccataatc attgcttgtc cggttgtggc gccttggatt tcagctatcc cagatgtatc
    23041 gatgttttct tttgtcagta gatctaataa ctgttgtcct tcgctgtcag ccccaacgcc
    23101 tccaataaaa gatgtccgtg cgcctgaacg tttggctgcc actgcttggt tggcaccttt
    23161 acctccgcca gcgtgaaaaa tctcatgcgc atgcatcgtt tcgcctggct tgggcatgtt
    23221 ggttaaacga agggtagtgt ccatattaat acttccaatt actgtaaccg tgttcatttt
    23281 acaaacctcc aactatctca tttattttga tgggaaaatt attttttaaa acgttttagt
    23341 aaaacgtttt aatagttcga taaaaatata gcacaccttg aaagctattt caagggtgca
    23401 gttgaaaatc ttttttctaa tcgtacaggt aatctttttt cttcccattc tttttctggg
    23461 aattggatcc gatctaacaa taacttggca gatgtttgtc ccaattcgaa gattggttgt
    23521 gcgatcgttg tcagtttcgg cttgatatac tcgcacatat ctatattatc atagccaata
    23581 atgctgtagt cttcagggat gctttttcca gcttcctcta atccgcgata taatccaaaa
    23641 gctaattcat catttaaagc aaacacacca gttgcagcag aatcaagaag ttctgctgtg
    23701 atctggtagc caccttgttt tgagaactgt gtaggtaaga ggatcagttg gtcgtgaggg
    23761 atttggtaaa catcaagagc agatttaaac ccctcaatcc gagcgtggac attttccggt
    23821 ggattttcag gatagacaag ggcgatcgtt tgatgtccta gactaagcaa gtgcatgccc
    23881 gctaggtaac caccgcgaaa atcgtctgtt cgtacggcat cactaaatcc ttcagatttt
    23941 ttctgatcca aaacaatgaa gggccggcct tgttttttca aattttcgtt gattgcatct
    24001 gtagagacag cagaagtcgc gataatgaat ccatccacac ctcgacgtgt gagctcggct
    24061 agatattcga tttctttttg atgatcagaa tcagcattac ataatatcgt gacgaaattc
    24121 tgcttataca agatatcctc aattcctcgc atcagtgtac tgaagaatgg attcgtgata
    24181 tcaggaacga gtacaccaat tgttttcgtt tccttggtaa tcatttgtct agcaaaatag
    24241 tcaggctgat agcctaattc ttctttggca gctaaaactt tctgaacagt tttcggactg
    24301 aatttctccg tatttccatt taatatcaag gagacagttg tgattgaaac gccggaaagt
    24361 ttagcgactt ctttgattgt gatctttttt ttcaccatgt tcatactcct atctaaatcc
    24421 tgtggttatt ttcccatgaa atcaaaaaga agtaaatgaa aatcacgtgt gccagtactt
    24481 atttgctttg ttggatagct tcgattattt gtttcgttga gcgtactcgt gcagttttcg
    24541 gaaatatctt ttttatgaac aattgatggg attctttgtc acgatcactg caagcatctt
    24601 cagcaagcac gatatggtac ccgtgttgaa aggcatctaa tgcagtagca tatacaccgt
    24661 ttgttgtcgc tacaccgctt aaaatgatag tttcgattcc acgccggcga agttgaagat
    24721 caagagaagt accaaaaaaa gcacttggat tgtattttgt gatagtcaac atattatctg
    24781 tatctttcaa agaatccttc aaaagtaaat cagtgaattc tggaggtaca gaagcagtca
    24841 ttccacccat atcagtttgt tgactcaaat aagggaaagt ggagatgtct acgttcacta
    24901 aaactttcaa ggcagcggta cgtttcaatg ttcttactaa caaatcattg tttgtcagaa
    24961 tagatgctgc agtgtgtgga gagagtgtac cgttatttac gatctctttt tgcatgtcga
    25021 tcgcgacaaa agcagttttt gataaatcaa aaatcatatt ttcctttaaa tcattcatga
    25081 tgttccttct ttcaaataac aatgtttttt atgtaagtaa atcataaatc gtttatttgt
    25141 caactaaatt aaaggattcc tttctttagt ctagagaaat tcagtggtaa aataaataag
    25201 tgtgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa tggaggaatc
    25261 agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg agaaacgacc
    25321 attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt gattatactt
    25381 ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat ggatacacct
    25441 gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga taaacagctt
    25501 actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct cgttcctaat
    25561 ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc gcttgatcct
    25621 gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc gtcccaatta
    25681 tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa tcccaaactt
    25741 cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca agtattgaaa
    25801 ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac acataattct
    25861 gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt aaggtctgtt
    25921 gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga ttttatgaaa
    25981 acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg acgattcatt
    26041 gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa aactgctgga
    26101 cctgtcatgc aaaaaacgat ggatcactat gttggtacag caggactttc tgatttgcaa
    26161 atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa gatcccagat
    26221 gcaaaagtag aaacaggaaa acagatctat tattccaata ctggcaaaaa cgaagtgatt
    26281 caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac tgatggaaaa
    26341 ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga agggtataaa
    26401 atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta taccatcacg
    26461 ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc gaacgtcgga
    26521 aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac agatatttat
    26581 tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag caaagcttac
    26641 aaagataaaa tggagcaaaa tcaagaaaag gcagaaaagt atttacaaga tcgacccgaa
    26701 gaacgcttag aagagctgaa aaaatcggcc aacgaagaat tagaaccggc acagcaaaaa
    26761 atagcggatg gcaaagcaca aacaacgcaa gcaagaacac ggttagaaga tgctaaaaaa
    26821 cagttagcgc aacagtcagc aactatccag cagcttcctg aagcgcaacg aacaatcgct
    26881 gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca attagttgag
    26941 aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga aaaaattgaa
    27001 aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg ttttcaagaa
    27061 tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt tttctttttc
    27121 tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga aaaccgcagg
    27181 gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa aaaatatgca
    27241 atctatgcaa gtctagcctc agggataggt attgttttag gaacgatatt aggaacgaat
    27301 cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg gagtgcgctt
    27361 attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat tgccgcattt
    27421 ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc attgcttcaa
    27481 ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc gctgtggtca
    27541 cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa gtcaagaatg
    27601 cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg cgtgggattg
    27661 aaagattctc taagttctgt ttcagccaaa caatttggac cgatcattga ctatcaagca
    27721 atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga tatgctgcag
    27781 aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga attgagaaaa
    27841 aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc tgatttggat
    27901 cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa aacaggaatc
    27961 gctattacgc aaaaagtagc agaactattt gacttatcta caggagatca tatttcgctt
    28021 tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa aaactatcta
    28081 ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca atcgatgatg
    28141 gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga aatgctttcc
    28201 gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa acaaatcgaa
    28261 acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt cgtattatca
    28321 ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc cgagcgtgta
    28381 cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac gatgtacatt
    28441 gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg tgtaggatat
    28501 atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt tccattagtg
    28561 atcacatggt gggcttatgt cttatcagca ggattaacta tcgtatttac ggttattgtg
    28621 atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt gaaatcgaat
    28681 gaataaaaac agaggttgtg aaatcagcgg tttgacctga gtattagaga aacaaacggc
    28741 aaaatagttt ttcatatttt gccgtttttt gagctaatac cgaaggacag ctgattgaac
    28801 accatgaatc agaggttgta aaaaagccgt cctgcatcga gcaaaaagaa tactcaaata
    28861 aaattagctc ctcatgtttt acatttgaga agactgattg ccgtagataa aagcttttga
    28921 acaccattta tagaagaaac aagaatattt tgctatttta aacaagagga tagacaaata
    28981 catgtctgat ctcttgtttt ttattgggca acagacctct tagaatgtat aaagataaat
    29041 tttacaaacg ctttcggttc agttataata ataaatgata acttgtatat ataagttgtg
    29101 gtaggtaata aatcagttga atggaggtaa atatccatga catttaaaga caaagtcatt
    29161 taccaaatct atcccaaatc ttacaaagac agtaatggtg atggaattgg agatttaaaa
    29221 ggaatcaaag aaaaactccc gtatctgaat gatctaggca tcgacatgat ttggctaaac
    29281 cctatttatc caagccctca aaaagacaac ggttatgata tctctgatta cacagccatc
    29341 gatcctatct ttggtacgat ggaggaattc gaacagctaa tagccgaagc aaagaaatac
    29401 cggatagaaa tcatgctcga tatggtgctg aatcacgttt cgacagaaca tgaatggttc
    29461 aaaaaagcac tagcagggga ccagcattat caggacttct tcattttaag agaagagcca
    29521 acggattggg tatcaaaatt tggtggaagt gcttggtcgc catttggaga tacggggaaa
    29581 tattatttgc acttatatga tcggacacaa gctgacttga actggcggaa ccaagcagta
    29641 agagaggaac tattcaaagt tgttcgtttt tggatggaaa aaggggtaaa aggattccgt
    29701 ttcgatgtta tcaatgttat cggtaaagac gaacaattga aaaataataa agaaaatgat
    29761 ggcaaacctg aatatacgga caaaccaatc acccatacgt atctaaaaga attgaaccaa
    29821 gcaacgtttg ggacggattc cactatcatc acagtcggtg aaatgagctc tacgacgatc
    29881 gaaaattgta tcttgtatac agaacctagt agagaagaat tatcaatggt ctttaatttc
    29941 catcatttga aagtcgatta tgaaaatggg aataaatggt ctactcctcc atttgatttt
    30001 gcagaattaa agcgactatt tcatacatgg ggagaaaaga tgagtgaggg taacggctgg
    30061 aatgctctat tttttaataa tcatgatcag ccacgtgcgc tcaatcgatt tgtagctatc
    30121 gatcagtatc gaaaacaagg agcagagatg ctagcaacca tgatccattt gaaccgtggg
    30181 acgccctata tttatatggg agaagaaatc ggcatgatcg acccagacta ttcatcgatt
    30241 tccgactata ttgacgtaga aagtttgaat gcttataagt tactccttga acaaggattc
    30301 acagaaaaag aagcgtttcg tcggatcaaa gcaaaatctc gcgataactc tcgaacaccc
    30361 atgcaatggt cagacacaga acaagcagga tttactacag ggaaaccttg gttaaaagtt
    30421 ggaggcaagc tagaagagat taatgtagaa aaagaactat cttctgaaga ttctattttg
    30481 tcctattaca agaaattgat tcggctgaga aaaacatatc caatcgttgc acaaggagat
    30541 tatcatgctt atgaagcaaa tcatccggaa gtttatggct ttcttcgcca atttgaagga
    30601 cagcagctgc ttgtattaac aaatttctat gataaagata caaggatcgc catcccagaa
    30661 ggattcacag aagccaatat tcttatcgat aattattccg atactgttct tcatcaaagt
    30721 ctggagctaa aaccatacca ggcaattgct ttgctgaaaa attaaaacaa aaacacaaaa
    30781 cgtcgttcct acgagctatt ctcgtaagat cgacgttttg tgttttattt aaaataatct
    30841 tcttcagaaa atgaagagtt ttcatactct cctttatcgc cataaaattt attgtagcgt
    30901 tgtttgaatt tacggaaaat aaaagtaacg agtaatttga ctaacgtata gataggaata
    30961 ccaaaaataa cgcccacaat tcccattaga tcacccatga caagtaaaac gattaagatc
    31021 gtaatcggat ggatctgtaa acgatcaccc attactcgag gaatcaccaa gtcaccgtgg
    31081 agaagttgta cggcaaacca gacaatgaca aatttgacag ccataaaagt agagtcttgg
    31141 aatgcaatga tcaaacctgg aaagaaggcg ataaacggcc caatatatgg aatgatacat
    31201 aacaacccag cagaaatagc taaaacacta gcaaattcta atccgatcag taagaaacaa
    31261 acccagtaga ctgctccaag gataaatgaa gcaatgattt gcccctttag aaatgaaccc
    31321 aattgttgat tagcaatctt tgtcaaattt ttaaaatctg tacgaaatgc tggtgggaca
    31381 atcgctaaaa ctgactgata aaatttttga ggatctctca aaaggaaaaa agcgacaatt
    31441 ggaccagtaa ataaagtaat aaaaatagct gtaacagttg aaaaaatgtt gctgatccgt
    31501 tgtgcgccac tttcccaata atttccaata aatccagcta tatcagacgt cacgtcgtca
    31561 atagaagcga aaaattgatt aaatgaatcg gcaaaaggtg ttttctccaa aaattgtcgg
    31621 agactctctt gaaaatcttg aaacaaatct ggcaaactac tgatcaatgt ctttgcctgt
    31681 tcttcaagaa aaggaataag ccatatgctt ccaagcccca ataaaaataa aataatcaca
    31741 taaacgataa tgactgacaa gttcctcgaa aggtgcttgc tcaaacgttt gatgactggt
    31801 tccaaaagat aatacaagat cagtgcaaac aataatgggg gcaagatagt agcgataatg
    31861 attccgacag gcttgaatat aaacgagatc tgatggaaaa aataaatgat cagcgcaagt
    31921 aagataacta ttcctaaaac atagtaagag gtttttcctc ctaaaaagcg gatcaaacga
    31981 ttcgttgtat ctgtttcttt tttcactctt tttcctcctt tatctataac ttcattatag
    32041 acaaaagcgg gtataaaagg aaaatgatag aggttgattg caaaaatatc gtcaatcatt
    32101 catataaact ttctcatcaa ttaaaataag aaaacgcttg acagttcttt taaccatatg
    32161 ataaattaaa tcctgttgct aaaacgtaac tattacggtt tgcgaaaagc gaatcggaac
    32221 attcaaatga aagaagatga gaagaggagt gtcataaatg aaacgattga taggtatatt
    32281 ggccatgctg gttttagcag gtatgttaac tgcttgcggg gcaagtggga aagccgagga
    32341 cagtaaagaa aaattgagcg tgatgacgac cttttatccc atgtatgatt ttacaaaagc
    32401 aatcgtcggg gatgaaggag aagtagagtt attgattcca gcagggactg attcccacga
    32461 ttatgaacct tctgctaaag acatggcaaa gatccaagat acagatatct ttgtttataa
    32521 tgatgaaaac atggaaacat gggtccccgc aatccaaaaa acattacaag aaggcaatgt
    32581 ccatacgata aaagcgacag aaggtatgct tttacttccg ggaagtgaag aaggacatga
    32641 tcatgaccat gaacacgggg aagaaggcca tacacatgag ctagacccac atgtatggct
    32701 agctccaagt ttagcaatca agcaggtagc aaacatacgt gatcagctga ttgaagctta
    32761 cccagaaaaa caggaagtat ggacaaaaaa tgcagcagcc tataccgaga aacttcaagc
    32821 actccatcag ctttatcaag aaacctttaa gcaagcaaaa caaagatctt ttgtcacgca
    32881 acatgctgca ttcaactatc tagcgttaga atatggattg aatcaagtat cgattgctgg
    32941 tctttcttct agtgaagaac cgtctgcggc gagaattgct gaactgaaac actttgttaa
    33001 agaacatgga atcaactata tttattttga agaaaacgcc aaagatagta ttgccagaac
    33061 gttagcaaac gaagcaggtg tatctttaga agtattgaat cctttggaag gattgacgaa
    33121 tgaacaaata gaaaatggag aaaactatct ttctatcatg gaagcaaacc tcgaagcgtt
    33181 gaaaaaaacg acagagacag aaaacccgct agaagatact ttgaccccca aaaaagaaaa
    33241 gagcgtctac aacggttatt ttgaagacga ggatgtacaa gatcgctctc tttcagattg
    33301 ggcaggaact tggcaaacag tagatacgta tgtagaagat gggacttttg atcctgtttt
    33361 tgaatataaa gaaaaattga atcaggataa gacagcacaa gaatataaag aatattatac
    33421 gaagggctat caaacagata ttgcttcaat caagatcagt gagaaagaga tggcatttac
    33481 gtttaagaat ggaacaacga aacgttcaaa ttatcgttat gtagggaaag aaatcttgac
    33541 ctacgaggca ggaaaccgtg gcgtaaggta tctattcgaa gcagaagata aggaaagtgg
    33601 cgcttttaga tatattcaat tcagtgatca cacgattgca ccggctgact ccgaacattt
    33661 tcatttatat gcaggaa
//
LOCUS       DAYYGS010000025        33111 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000025 DAYYGS010000000
VERSION     DAYYGS010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.025"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(136..516)
                     /gene="yajC"
                     /locus_tag="ACHA44_001084"
     CDS             complement(136..516)
                     /gene="yajC"
                     /locus_tag="ACHA44_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFM8904393.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(613..1758)
                     /gene="tgt"
                     /locus_tag="ACHA44_001085"
     CDS             complement(613..1758)
                     /gene="tgt"
                     /locus_tag="ACHA44_001085"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFM8904394.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1976..2674
                     /locus_tag="ACHA44_001086"
     CDS             1976..2674
                     /locus_tag="ACHA44_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HFM8904395.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2842..5481)
                     /locus_tag="ACHA44_001087"
     CDS             complement(2842..5481)
                     /locus_tag="ACHA44_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8904396.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6100..7131)
                     /gene="queA"
                     /locus_tag="ACHA44_001088"
     CDS             complement(6100..7131)
                     /gene="queA"
                     /locus_tag="ACHA44_001088"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFM8904397.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7324..8499
                     /locus_tag="ACHA44_001089"
     CDS             7324..8499
                     /locus_tag="ACHA44_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8904398.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8503..9144
                     /locus_tag="ACHA44_001090"
     CDS             8503..9144
                     /locus_tag="ACHA44_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904399.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9141..10058
                     /locus_tag="ACHA44_001091"
     CDS             9141..10058
                     /locus_tag="ACHA44_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8904400.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10055..10726
                     /locus_tag="ACHA44_001092"
     CDS             10055..10726
                     /locus_tag="ACHA44_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904401.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11267..11755)
                     /locus_tag="ACHA44_001093"
     CDS             complement(11267..11755)
                     /locus_tag="ACHA44_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HFM8904402.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11956..12222)
                     /locus_tag="ACHA44_001094"
     CDS             complement(11956..12222)
                     /locus_tag="ACHA44_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8904403.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12222..12485)
                     /locus_tag="ACHA44_001095"
     CDS             complement(12222..12485)
                     /locus_tag="ACHA44_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8904404.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            13006..13146
                     /locus_tag="ACHA44_001096"
                     /pseudo
     CDS             13006..13146
                     /locus_tag="ACHA44_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(13288..15111)
                     /locus_tag="ACHA44_001097"
     CDS             complement(13288..15111)
                     /locus_tag="ACHA44_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8904405.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15709..18414)
                     /locus_tag="ACHA44_001098"
     CDS             complement(15709..18414)
                     /locus_tag="ACHA44_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HFM8904406.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18720..19667)
                     /locus_tag="ACHA44_001099"
     CDS             complement(18720..19667)
                     /locus_tag="ACHA44_001099"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HFM8904407.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19857..20126
                     /gene="rpsN"
                     /locus_tag="ACHA44_001100"
     CDS             19857..20126
                     /gene="rpsN"
                     /locus_tag="ACHA44_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8904408.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20302..20664)
                     /locus_tag="ACHA44_001101"
     CDS             complement(20302..20664)
                     /locus_tag="ACHA44_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8904409.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20675..21796)
                     /gene="alr"
                     /locus_tag="ACHA44_001102"
     CDS             complement(20675..21796)
                     /gene="alr"
                     /locus_tag="ACHA44_001102"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFM8904410.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21812..22162)
                     /gene="acpS"
                     /locus_tag="ACHA44_001103"
     CDS             complement(21812..22162)
                     /gene="acpS"
                     /locus_tag="ACHA44_001103"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFM8904411.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22292..23803)
                     /locus_tag="ACHA44_001104"
     CDS             complement(22292..23803)
                     /locus_tag="ACHA44_001104"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFM8904412.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24125..25477)
                     /gene="murF"
                     /locus_tag="ACHA44_001105"
     CDS             complement(24125..25477)
                     /gene="murF"
                     /locus_tag="ACHA44_001105"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFM8904413.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25630..26706)
                     /locus_tag="ACHA44_001106"
     CDS             complement(25630..26706)
                     /locus_tag="ACHA44_001106"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFM8904414.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26902..28647
                     /locus_tag="ACHA44_001107"
     CDS             26902..28647
                     /locus_tag="ACHA44_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HFM8904415.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28927..29364)
                     /locus_tag="ACHA44_001108"
     CDS             complement(28927..29364)
                     /locus_tag="ACHA44_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFM8904416.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29348..30721)
                     /locus_tag="ACHA44_001109"
     CDS             complement(29348..30721)
                     /locus_tag="ACHA44_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904417.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31043..31531)
                     /locus_tag="ACHA44_001110"
     CDS             complement(31043..31531)
                     /locus_tag="ACHA44_001110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904418.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31553..32155)
                     /locus_tag="ACHA44_001111"
     CDS             complement(31553..32155)
                     /locus_tag="ACHA44_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8904419.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
     gene            complement(32224..>33111)
                     /locus_tag="ACHA44_001112"
     CDS             complement(32224..>33111)
                     /locus_tag="ACHA44_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HFM8904420.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     WVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT         9458 a         7033 c         6062 g        10558 t
ORIGIN      
        1 attattattt atatgacaca cccgattatt caatattgag caatatatcc catgctctta
       61 tctctttctt ttttgaataa aaaatgaggt aggacatctt tctgccctac ctcgtcttta
      121 aataagcttt gatacttatt tttcttcctt ctcttctgtc tcttccagcg tttctgttgt
      181 ttcagttttt acttcaactg tctcagctgg ttcttctgtt gtttcaacag tagtcgttgc
      241 atcatttgtt acgacagtcc ctggtttgac cgttttgatt gctgcacgat cgtactcaag
      301 gaagatccct tcgcagtcta aaacaactgt ccgtttgtca ttgtcgattt ctgaaacaac
      361 tccatgtaat ccgccgatcg ttacgactga atcacctggt ttcatgctat ctaacaagtt
      421 ttgacgttct tgctgttgtt tcttttgcga acgtgacata aaaaaccaca ttgctaatag
      481 cgcgataaac atcacaagca ttggtaatgt tcccattttt ttcacctcac taattctgta
      541 tctacaaaaa acactttagc agaaaaatgc tgataacact agttttttac ttaattttta
      601 aaagattttc ctttagaagc tttttgcgtt ttctttattg aacccgtatt cttcaaaaaa
      661 ggcttctcta aattcaagca aattatcatc catgattgct tgacgtactt gtttcattag
      721 attcaacaag aagtataaat tgtggtaaga agtcaaacga atcccaaatg tttcatcaca
      781 tttgatcaaa tggcggatat aggcacgtgt atagttttta catgtataac aatcacattt
      841 ctcatcaatt ggacggaaat catgggcata tttcgcattt ttcactacta gacggccttg
      901 agaagtcata catgtgccat ttcgagcgat tcgagtaggc aagacacagt caaacatgtc
      961 tactccacgg ataacaccat cgatcaatga atcagcagca ccgacaccca tcaagtaacg
     1021 tggtttgttt tcggggatca acggtgtagt gaattctaat acacgattca tttctgattt
     1081 aggttccccg acagataacc cgccgattga gtaaccaggg aaatccatcc cgactagatc
     1141 tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct tggatgattc caaataatcc
     1201 ttgacggtct ggattagcat gagctttcaa tcctcgttct gcccatcgag acgtacgttc
     1261 tactgatttt ttcacgtagt cgtaactttc atcgaatggt ggacattcgt caaaactcat
     1321 catgatatca gagcccaatt tattttggat gttgattgct ttttctggtg ataaaaacat
     1381 acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct tcttcgatat tccgcatatc
     1441 acttaatgaa aaaacttgaa agccgcctga atctgttaaa atcggttgat cccagttcat
     1501 aaatttatgc agtccgccag cttcttctac taagtcttca ccagggcgta gccataaatg
     1561 atatgtatta cttaaaataa cgccagcacc catttctttc aactcttctg gggacattgt
     1621 ttttactgta gctaatgttc ctactggcat gaacattggt gtggggaaag ttccgtgtgg
     1681 cgtgatcaat tcccctaaac gcgcgccagt atgtttttct ttcttgatta aacgatagcg
     1741 aatggcaggt tgcgtcattc atttccctac tttctatata aaatatcttg acaactccca
     1801 ccatcataat cgtttcaagt gctttttgca agttccctcg gcttccttct actttattta
     1861 atattctttt ttttatcctt tttttatgaa taacaaaaaa aagaaaaaca ttatgacaga
     1921 aatatttttt gatacatggt atattggtgt tattaaagaa aaggagggaa aaatcatgca
     1981 atcttctgca gaattaaaac gccaagcaaa agagagtcta aaaggacgtt ggggacaagc
     2041 tgttttacta aatctaatcc ctacattgat tggtattgct actattcttc ttattatgat
     2101 tcctattatc ttgtttgcca ccaccatata tgatggatca gcgagcgtct cagaagcgac
     2161 aacttcagga ggcggtagtt caggaggcgg tggcggaatt atttccagca tcattagcgc
     2221 tttgttcatg agcggtattt cttggactta tcttgatatt ttacgtggac aaaaagatca
     2281 gatcgaacca ttcaaagatg catttcgtgg attcaaaggt ttgtttatcg gcggagtgat
     2341 cagcttagct gtgctgatct cgatctttac aacattatgg ggtttgctat tagttattcc
     2401 tggaatcatt aaaagctatt cttattctca aagctatttt atttactacg atatcgtcca
     2461 agaaacagga caaacaccta aagtactgga tacgatcact gcaagtcgaa aattgatgga
     2521 tggttataaa ggacaacttt tctggttaga tctttcattt atcggctggc atatcttagc
     2581 attagcaact gctggtattg gttatctatg gctcagccca tacatttctg caacaaaagc
     2641 agcattttat gaacaattgc caaaagatat ttaaaagaga gtgtgacttg tgaccgaggg
     2701 ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga aacattcagc ccattagcaa
     2761 aaaggaggtg tgacaacatt catgtcacac ctcctttttg tatagttcca gtaaaaagaa
     2821 aatcaactta gaaacagtta atcattcctc ttcttctgct tccggtaatc gatggaactt
     2881 gatcagttca ttcaccgcga ttggtacgat agataaaatc cctaccaaga accagtgatt
     2941 caaactgatg tgtactaggc cgaatagttc ttgcgtaaat ggcagcaaag caacagccgt
     3001 tgtaatagct aatgccagta acgccatttc taccaaagct ttattggtcg ctaaattgac
     3061 tttgaagatc gattggctgc tgcgtacatt gaacacatgc agaatcgatg aataagctaa
     3121 gatcaagaat gcaacagttt gaccgacatg ctgacttgcg tctacccatg ggctgatagt
     3181 atcgacataa ctacctaaat aataaccaaa caaggttacg atggtgaaga cccctgcatt
     3241 tatggcaatt ttttgcaata aacctcgagc aaagatccct tcatttttcg gaatgggtgg
     3301 ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa gcaaatcctg gaagtccatc
     3361 agcaactaca ttgacaaaca gtagttgcac agctgtaaat ggtgctcccc atcctaacag
     3421 catcgcaatc aaaacaataa agatctccga aatattacag cttagcaaaa agttgatagc
     3481 tttacgaata ttctgataaa ccgaacgtcc ttgactgaca gcgtctacga tcgtcgcaaa
     3541 gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc acatctgttc ctgtgatccc
     3601 catggcacac ccaacgttac ttgctttcaa cgctggtgca tcattcacac catccccagt
     3661 catcgctacg actgcacccg tacgttgcca agatttgacg atccggatct tgtcttctgg
     3721 cgttacccga gcatagacag agagagcttt gacacgcgca tctaattctt cgtctgataa
     3781 ttgatgcagt tctgatcctg aaagtgcttc tgacggatca ttcaagatac ctaattcttt
     3841 cgctatggca ctggcagtca ccacatgatc tccagtgatc atgactgttt tgatgccagc
     3901 ctttttcgct cgcttgattg ctcctttact ttctggacgt ggtggatcga tcattccgat
     3961 cagaccaatc aaacgtaagt ctttttccag tgcttcagaa gaaatatctt gcggttcttc
     4021 gtcataagta gcataaccaa cagcaatcac tctcagtgcc cgtttaccaa agcgatcatt
     4081 aacgatcgcc gcttgttcta catcgcctga actaaagcgc ggagcaagaa catcgaatgc
     4141 tcctttggtg attgagatgt atttttcgcc catttggtga actgttgtca tcatttttcg
     4201 ttcagaatca aaaggaagct cgtttactcg tggatatttt tcttctaatt ctgcctttgt
     4261 atgataattc tcttcaacag cccttacgat ggctacttct gttggattac ctttgataac
     4321 aaggtcgtca tcttcttgct cgaccgtcac atctgtacat agtgcagcca ttttcaatat
     4381 ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt acttcatctt ctttgctcca
     4441 tacccggcgg acgcgcattt cattttgtgt cagcgttcct gttttgtctg agcagataac
     4501 attagcggtt cctagcgttt caactgctgg caactgtcgg atgatcgcat gtttcctcgc
     4561 catcttctgc acaccgtatg caagtgtcaa cgtaacaata acagtcaagg tttccggtac
     4621 agctgcaaca gccaaggaaa tcgaagtcat gaacatctca aggagtggtt ctccttgcag
     4681 ttcaccaatc acaaatacta aagccgcagc cgctaaagcg atcatactga ttcgtttgcc
     4741 tagctgattc aatcggacct gtaaaggtgt tttttgcata gtattttcgt tcaaaagatc
     4801 cgcgatctta cccatttctg tagccatgcc aatggcagtg acgatcgctt tcccacgacc
     4861 tgcagcaacc gtacatcctt taaataccat attaagttgg tcacctaatg actcatcttc
     4921 ttcagcaata aatgctgcat ctttttcaac tgcttcactt tctccagtca acgccgattc
     4981 ttctacacgt aattgtgatg cttcgatcaa acgggcatcg gcagaaatag catcaccgga
     5041 ctctaaaaca agaatatcac ccactaccag ttgctctgac tcaacagtgg tcactttgcc
     5101 gtctcgtatg acagttgccg tttgtttatt catatcttgc aaggattcca gtgctttttc
     5161 tgcgtttcct tcttggacaa tcgccaatac cgagttgatc acaacgatag cgatgatcag
     5221 cagtccttca aataaatccc catgttctgt agcaaatgcg gtataaaagg aaatcaccgc
     5281 tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt cgaaagaatt ttttgatcat
     5341 cgattctttc ggcgcctcat cgaatttatt caatccctgc ttttttcgcc gttcatccac
     5401 ttcttgtgaa gacaacccag tatctggatc tgcttctgtt accttcttta cttcttctat
     5461 tgtctgcttg tattcttcca tcctattcct cctttaaaat cttcgtacaa aaaaaggtac
     5521 ttattttact ataacatcct ttttttattt acttcaaata aagggcttat cgattatttg
     5581 ttaagaaagc gtaaaattta aagtgcttat atttgcaaac gctaccaaag gatgctatta
     5641 tatagatgta aggaaggagg ataccatcaa atagacagaa gaatttctcg atagaacagt
     5701 gtaagcaaac aaagttgtta gaagtacagg tttcacccag acatgcgtgt agagtacaaa
     5761 cctataaata gtgaatgcgt atggatgttt ggatcacgaa gtatccgaaa caagaagtaa
     5821 tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat tgaataggaa caagagatgt
     5881 ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt aacatgaaga aattcaacac
     5941 cgtgctttgc atgtatgatg gaaagttaca atcatttctg tttatttgaa tccttccgag
     6001 gtacaagaaa gccgagctta tcgagagctc ggctttttct ttgtcataaa atctttgtaa
     6061 agcttttttg ttattcaaaa ctagcggctt ttcataacct tatttcacga acatcgcatc
     6121 tccaaaacta aagaaacgat agcgctcttc aatcgcatgc tggtaagcag ataaagtcaa
     6181 ttctcttcct gcaaaggcac taactaacat gactaaagtc gattttggca aatggaaatt
     6241 agttgagaaa gcttctacca ctttgaattc atagccaggc gtaatgaaaa tatcagtcca
     6301 tccgctgtct gctttgattt ctccattaaa tttcgttcca atcgtttcta atgttcgaat
     6361 tgaagttgtt ccgacagcca cgattttccc gccattttta cgtacttggt tcaatcgctc
     6421 agccgcttct tccgtcaaac ggtagaattc actatgcata tggtgttctt caattttatc
     6481 tacgctgaca ggtcggaatg tacctaaccc tacatgtagt gtcagataga ctaattcaac
     6541 accctttgct tcgatctcat caagtaattc tttcgtaaaa tgcaatccag ctgttggcgc
     6601 tgcagcagaa ccattttctt tcgcatagac agtttgatag cgatctgggt cttccagacg
     6661 ttctttgata tatggaggca atggcatttc tcccaatgat tcaagaatct ctagaaaaat
     6721 tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc aattcttctt tcactactgc
     6781 ttgcaatctg ccatcaccaa aagagattct tgtcccaact ttggcacgtt tcgcaggttt
     6841 gatcaatgtc tcccacgtat ctccttgtgt attcgttaga agaaggactt ctaaatgcgc
     6901 accagtatct ggtttttctc catataaacg agcaggcaga actcgtgtat tattcataac
     6961 tagcgcatca ccaggctgta actcttcaat gatttcatgg aaatgtttat cttccatctc
     7021 tccagttttc cgatccaaaa caagtaaacg agaatgatcg cggtctttta atggtgtttg
     7081 tgcgattaat tcttccggca agtcaaaatc aaaatcttct gttgttaaca tatcgtcact
     7141 cactttctat catgtgcttc atcattctat catgtttttc gttctgttgg caaaatcagc
     7201 actttttcac ttctattttt tgttttccta aataaaaaaa ctaccctatt tgtatttttt
     7261 cattgtttta ggcatactag taatagatag ttcttcattg aaaatagaaa aagaggtggc
     7321 accatgattg aatttcaaaa tgtgtcaaaa atttataaag gtggaaaagt cgctgtagag
     7381 gacgtcaatc tttcatttga aaagggtgag ttcatttgtt ttatcggtac gagcggaagc
     7441 ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg atccaacaca agggaaaatt
     7501 ttgattgatg gaaaatccat ccaagaaatt gatccagtgg aattgcgacg tcagatcggc
     7561 tatgttatcc aaagtattgg attgatgcct cacatgacga tccgtgaaaa tatcactctt
     7621 gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata aaacagctga aaaaatgatt
     7681 gatcttgtgg aattgccccg tgagatgtta gatcgctacc cacatgaatt atctggtggt
     7741 cagcagcagc gtattggagt cgttcgtgcc cttgcagcga atcaagatat catcttgatg
     7801 gacgagccat tcggtgcgct tgatccaatc actcgtgatt ccttgcaaga ccttgtcaaa
     7861 gatttacaag agcgtttggg aaaaacgatc gtatttgtaa cccatgacat ggatgaagcg
     7921 attaaactag caagccgtat cgccatcatg agtgaaggcc gtgtgatcca atttgataca
     7981 ccagaaaata ttttacgtca tcctgcaaac gatttcgtag aagagttgat tggagaagat
     8041 cgcctactac aagccaagcc aaatgcgaca agagtcggcg aagttatgct aaatacagca
     8101 atcacgatta caccggaaaa atcattacaa gaagcaatca aactgatgag ggaaaaacgt
     8161 gtagatacgt tgttagttac cgataattcg aatgtattaa aaggatttat cgatgtagaa
     8221 acgttgaata aaaaacgagg aaaagtatcc agtgtcggcg acattttgaa taaacatgtt
     8281 ttttacgtaa aagaatccgc ctatcttcgt gataccttgc aacgcatttt aaaacgcggg
     8341 ctgaaatacg tgccggtcgt tgatgaacag aacaaagtcg taggtatcct gactcgtgca
     8401 tcattagtgg atatcgtcta cgatgtgatt tggggagaag atcccgcaac tactgatgtt
     8461 gcagagtcag ctactcctga agaatctaag gaggtctgac ctatgcagca gttttttcat
     8521 cagcacggtt cagaaattct ctcaaaaggt tttgaacatc tttatatttc aggacttgct
     8581 ttgatcattg gtatcatcat tgctgtaccg cttggtgtct tactgacacg ttttccaaaa
     8641 gctgccacga ttgtgattgg cttaaccagt gccttgcaga caatcccttc tcttgcttta
     8701 ttggcattga tgatcccgct atttggcgta ggtaaattac ctgcgattat cgcactcttt
     8761 atttattcat tattgccgat tttacgtaat acgtacattg ggatgaaaaa tgtcgattgg
     8821 aattatcggg atgtggcaaa aggcatgggg atgactgagt tccaatccat tttttccgtt
     8881 gaattgccca ttgctatgcc aacgatcatg gctggtattc gtttagctgc cgtttacgtg
     8941 attgcatggg ctacacttgc ctcttatatc ggcgctggtg gtttaggcga tttgattttt
     9001 agtggactga ataattacca accagatctc attttggcag gtacgatccc tgtaacaatt
     9061 ttagcactat tggctgattg gcttctaggt ctcttggaac accggctgac accgatcgca
     9121 cttagagagg agaatgaaga atgaaacgta tactaaaaat gaccatggtt cttttctctc
     9181 ttcttatact tgcgagttgt tccttccctg gtcttgccag caatacagat gaagacacca
     9241 tttcgatcac aggtggaatc acttcagaag cacagatctt agcaagtcta gttgccggaa
     9301 tggttgaaca ttatacagat aaaaataccg caatcatcaa caatctggct actacaacta
     9361 tcaaccatca agcgatgatg aacggagatg cttcaatttc agcggctcgc tacacaggaa
     9421 cagatttaac gactacattg aaccttcctc ctgaaaaaga tccgaaaaaa gcgtttgcta
     9481 ccgtgaaaga tgaatttgag aaaagatacg gtcaaacgtg gttcccgtcc tatagttttg
     9541 aaaatacgta tgtctttctt gttcgaaaag acaccgcaca aaaataccat ctatctaaag
     9601 tcagcgatct gaaaaatgta gcggatgaat tagtagctgg tgtagatact tcttggatca
     9661 atcgtaaagg agacggttat gacggcttcc aagaaactta tggcttctcc ttcaattcga
     9721 tattacctat gcagattgga cttgtttatg acgcagtcga agcaggaaaa atggatattg
     9781 ttcttggcta ctctacggat ggacggatcg ccagttatga tctagtcatg ttggaagatg
     9841 atcgtcaatt cttcccacca tatgatgctg ctccagttgt agacaacaag atattagctg
     9901 atacgcctca tttaaaagaa gccttaaata agttaggtca taccatcagt acggaaaaaa
     9961 tgcaggaatt gaactacgaa gcagataata atctagtgga accatcagta gtagcacaac
    10021 gtttcttaca agaaaaccac tattttgaag ggaagtgaag caattggata aaatgaatgt
    10081 ctttcaacaa tttttctatt atttccagga aaatggctcc tatatctttg cacaatttat
    10141 ccgtcacttc ctgatttcta tctatggtgt cctctttgca gcagttgtcg ggattccggt
    10201 tgggatcatg atttctagga gacgtaaatt agcaaactgg gtgattcgtt tggcgaatat
    10261 catccagacg atcccttctc tagccatgat ctccatttta atcattggat taggcctggg
    10321 agtcaatgtc gtgattgtta ctgtattttt gtattccctc ttaccgatca tcaagaatac
    10381 gtataccggg atgatccaag tagacaaaaa catccttgat gtcggcaaag gcatggggat
    10441 gactgctaga caacgactat tcatggtaga gttaccactc tctgtttctg ttatcatggc
    10501 aggcataaga aacgcgttag ttgttgccat cgggataacc gcgatcggtg cattcgtcgg
    10561 tgctggtggt cttggagata tcattatccg aggaaccaat gcaacagagg gcacatcgat
    10621 tattttagca ggtgcattgc caactgcatt aatggcgatc atcacggact ggcttttagg
    10681 tattttggaa agacggctag atccagccag ccgtacatca cgctgatgtt attgtaaaaa
    10741 acaaaagctt ctgcgaccat ttgttggtcg caaaggcttt tttagttctg tcatttttaa
    10801 ggcaataatc cactctgatt cttccctcct tatgggcaaa gagttgcttt tcttagctat
    10861 ttatccattc cgtatactcc aacttcatac tgccaacggg ttcatatctg catccaccga
    10921 ctaagttttt ttcttatccg ctaaggcatt ttgccaatcc attttcccat cactcgtcat
    10981 ggaattcata ccacatgatc tctaaatact cgtctgcttg ttctttgaat tgggcatcaa
    11041 ttcttacatc ctttggcgtg gctttcattt aagccttttc catcagtcct cgattgttcg
    11101 atgccttcag ctgcatcatc aaaactggtg actgccgtat cggtaacttc gatcatcttt
    11161 tatacaatcg ggaacttgcc atcctcatcc ttggctaaaa acatatctag ccattccagc
    11221 ttctcgccat gggaggtttg cggttcctaa aaccggtaca cgattcttat ggactgcatc
    11281 cgtcgcaact agacttggcg atacgatgat ccttcaccag aagagcaccc ccgtaaacca
    11341 attgatcgat atattgccaa taagaaaaga cattggcgta aaagatatcg atccctcgtg
    11401 atgcattgcc gttgatactg ctattcatgg ccgaaagcgc cacgacttta acttcttccg
    11461 tttgagtgac attcatgttc ggcaatgtcg ctttggatac ccgctctgct aaaggaacga
    11521 cactggtatt gtatttcgta tctgtatctt ttccaaaaga ccatgccaac gaatcctctg
    11581 ggaaccaata agacgactcc ggttgattgt ccattgcttt tttcaacata cttttggctg
    11641 tttctcgata aacgacattt ccaattgtct tcgcctcact ttggcgataa tcgatggtcg
    11701 ttgccactgc caacactaac aacgatgctc caattttact gattttcctt gtcacacttc
    11761 ttcctccttg ttttacataa acccgtcaac attccattcc taaactcaaa caatgaccct
    11821 tcaaaggtgg agatatagga acaaattaaa gaaatcatgc aataaatcca agaaggttgg
    11881 ttttagaaca aaaaaagagt ctcctttttg gggagactct ttgttaacta tctaaaaagg
    11941 ttggctcttc tcacttcact cataatgtgt ccaggcagaa taaatttcta caactttatt
    12001 ttcctcatca actttgtaaa ccactcgatg ctgtctattc agcctgcgtg agtatctgcc
    12061 ttcatgtgta ggtctcagct tttcaaaaga ctgcgtgggc atatatggat cttccttcaa
    12121 tgtctgaatc acttcttcaa attgtttctt taaattcgtt tgcttgattt ttcttaaatc
    12181 cactttcgca gagtttttga ttgcgactgt atagttactc atcaaagagc ttcccaatca
    12241 atatcatcga tattcgtaaa gctactgtcg tctttttcgc ggtctcgtac tttatctaaa
    12301 gtacctgttt gctcaagaaa aagggtttct tgaatcgctc tccagtcttc cagcccaatc
    12361 aatacagcat tgtttccact acgatcacta ataatctcga tttcagtatg attttcattt
    12421 acctctttca ataactgata aaaattttta cgagcagtgc taggattcat cgttgtatta
    12481 gccattgtct ctcctccttt ttctgatata attatagcgt acttgattaa gtacgtcaaa
    12541 aaaatacaat aaagcactta aattcagcaa acttttcctc ttttttacat agagaaaaag
    12601 accccttttt agggaggatc tttgtccacg ctcacttctt actcctttac gactagcggt
    12661 agccaaacgt aaagtaattg gtaacaacgt atcatcttta gcaaaggtcg aatccaaagc
    12721 ctgtgagtct atgcaacaac accaatacac gaaggttcct cgttgcagtg tgacttgatt
    12781 gatatcttga ataacctgag gatacgcacg aaagatttgt aacggttatt caaaactgat
    12841 tttggccgtc aagtcttcta ctgcctcaaa aatgattgcc tctttcgtct cctgtcattc
    12901 atgttcacta tcgatttgat acttgcttag accgttgggt ttgtgaatca ccaattaaaa
    12961 atggcactga gttatggtaa caaaaaatgg tcacttgacg aggctgagat tcctacttat
    13021 gaagacacat ggcacttaag agtcgaggta aaggaagaag ctgaaaatat aggaggctgt
    13081 acattatttg gcaaaggatt cggaaattat gatcaagaca tcaaaatcaa aatgttttac
    13141 agctaaatag gaagaataat aacatttcat acgctgttcc catctttaat ggcttataga
    13201 gggcaccttt agctgagaat aagacaaaac agaccctcta gcttcaatta agagctagag
    13261 ggtctgtttt tgattttaaa acttggttta tttcttcaaa tggtagctat aggtgctgac
    13321 aacgatattt tcacgccaag acaatcgcaa tcttaaccgc aaacttgttt ggttatggag
    13381 aatattctgc cagcgtctgt ttggtacaaa ttgaggaatc aacacagtag ttgtatagtt
    13441 ttgtttcgct gcatttttac ttacgatatc tacataacgg ataattggat tttcaatcga
    13501 gcgataagaa gagtggacga ttgaaaaacg gacatctggg aaatgctttt taaattctgc
    13561 ttcgatttct ttttcttttt cgacatcttc gtctagcgaa acatgcatcg caacgacata
    13621 atcaccgatc gaacgtgcgt aattcaacgc accgatattt acccgtgtca cattcccgac
    13681 taatacaaga acggtattcc cgtcataatc atgtagtttt gcatcatttt ctaaacgaag
    13741 ctgttctgct acttttttat aatgatcatg gatcttataa aaaatgaaca taacgattgg
    13801 cataatgatg aagaaaggcc aaatgtcccc taaccgatag acaaacaaaa tcgcaatgat
    13861 tgcatatgag atgaacgcac ccgtaatatt ggcaatcgat ttgcttaacc atctttttcc
    13921 ttcttttttc catttgacga ccatccctgt ttgagataaa gcaaatggaa tgaacacgcc
    13981 gatagagtat aacggaatca agcgttctgt tgagccatgg aaaataaaca gcaatacgat
    14041 cgaacctagc gctagtgtga taatcccatt ggaatagcct agtcgatccc cacgatcttg
    14101 atacatgtga ggcatgaatt tatcttttgc cagattgtat gcaagaactg gaaaagcaga
    14161 aaaaccggtg ttagcagcaa ctgctaaaat cagcgctgta gcaaattgaa ggacgtaata
    14221 caaaatacca tgtccaaaga cagctttacc aatttgagac aaaacagtga cttctttttc
    14281 aggaacgatc ccataccaat agttgataaa tgtgatcccc acgaagaaga aacctaagat
    14341 caatgccatc atcgtcaatg tagcagcggc attttttgct cttggcttct taaagaatgg
    14401 tacagcatta ctaatcgcct ccaccccagt taaagaggaa gaaccagaag aaaatgctcg
    14461 taaaatcaaa gcgatagaaa cacccggcac agccgctcct ggaagtgcag tagcttgcaa
    14521 tggcacagca cctgtcacga tattatataa accaacaacg atcagtaacg agatcacaag
    14581 gataaatgtg tacaccggaa ataataggaa cgatgcagat tccctcagcc cacgaagatt
    14641 cagcatcatc agtagcaaaa cgattgtcac agaaatagcg acttgatgac cgtagagtgc
    14701 tggcaccgct gatgtgatcg cttcagcacc tgctgagaca gatactgcta ctgtcaacat
    14761 gtagtcgatc aataaagagc caccggaaat caagccagca ttctttccaa gattctcact
    14821 gctgacgaca tatgctcccc cgccatgagg ataagcatgg atgatttgac gatatgatag
    14881 cgttaatgaa atcaataaaa taataacaaa agcggcaatg ggtagcgaat accagatggc
    14941 tgcagcagac aaagcgacta aaacgacaac gatttgttcc gtaccatatg caatagatga
    15001 taacgcatca gacgacaata atgccaaagc agcaaatcgg gttaatttgt gctcatcatt
    15061 ttcagcagat tttaaaggct tacctactaa caaccttttt aagtaatcca atttctaaac
    15121 tcctttacac acaattttac ttcaagtttt atagtcgaaa atttatagta aacaagatgt
    15181 tatagtacgc tcactgacct taaaagtcaa taaactttaa gaaaataatt ggaaaaaaac
    15241 agaaaaatat caagcgcttt ttcaaacgct ttaatacagc aaaaatctaa cattttttta
    15301 tgggcttttt ctttgaattc tataataaaa ggatagaact tgacttagaa aatcaagttc
    15361 tatccttttt gatatcttaa cttttggaag agagatgtaa ggctgctttc tacccatcat
    15421 tcttactact cgaagcatag cgcagttgtc acaacctctt gataaacggt gtttaaaagc
    15481 ttgcatctgc ggtaataagc ccaaccaaat taaaaacata agaagctgtt tttatttggt
    15541 cgttcttatt acttgatgca gagcagcttt tttacaacct cttggtacac ggtgttcaat
    15601 cagctgaccc tcggtattag ctcaaaaaaa cggcaaaata tgaggaacta tttttccatt
    15661 tttttctcta atactcaggt caaaacactg atttcacaac ctcttttttt aatgaaaaat
    15721 atggctctca tgcgccgctt ctttgaattt cgtcattttt tcttcgatga acggatagat
    15781 tgccgttcct acgattccaa agacaacaaa caaaccgatc atgatccaga ttgctttcgt
    15841 agcatttggc caataaattc ctcctgcaga ttcccgtaac aagtttaccg catgagtaaa
    15901 tggcaagaac ggattgatca tttggaagaa tttccctgag acttggatcg gatagttccc
    15961 gcctccgcca gagatcgaca gtactaagat gataatagcg atccctttac cgacattatc
    16021 gaataatgca gccaagacat agacgatcat catgaacgct aaggcgatca agacagcaaa
    16081 tgacacgctg tagaccggat tttggacatc tactcctagt aagaaaatat tccctaatgt
    16141 aacgatcaag ctttgcgcaa tcgccattgt caaaaatgtc agcatacgag caacgaattg
    16201 ttctcgtttc gtaaaattgt ttcggtcttt ctttcctaaa tagtattctg tcgtagcaac
    16261 acttgaaaga agaacagcgc cgacccacag acatagtgca gtataaaacg gggtgctagc
    16321 tgaaccgtta tttgcgattg gataaagcgc attggtttgt agctcaacag gttgtgtaaa
    16381 gaagtcactt tcttttgttg cgtctagttt caataatttg atgacttcgc ctagatcgac
    16441 ttcttgttcg ccttttcgga tcgcgtctgc tgctttttgg ataccagctt tgatatttgg
    16501 ccagtcattg ttgatcaagt ccgcagcagc gttcacagct gtttcgactt ctggcatttt
    16561 ttcatttact gttgtcaatg taccggtgat ttcatttttt agtgctggcc aatcattttg
    16621 gataaaatca gcggccaatc ctaatttttg ctgaatgatc ggtaattcat tgttgtatag
    16681 gtctgcccct tcattgatgc cattgacgat ggtatccata tttccattca gcattgtatt
    16741 ggcatcatgg atttcttgtc caattgcagg catttctttt tgatattttt ctaaaattgc
    16801 cactgcattc gttacagttg ctttcgttga gctgagaagc gaatcaaagt cgatctggtc
    16861 cgctttgctc agtgcgcctt gtgcagtccc aatcgtatcg atgacttggt ctagtagaga
    16921 agtgacagtg gaactgattt gacctgtatt gataccagca atgatgccat tgatattatt
    16981 aacaacggca tcaatttgat ctagcaattc atcaatagcc ttcgtgtctt cattttgaac
    17041 agctgtgttc aaagcagata ggcggttttg cagattgctt agcaagggtt ttaattgatt
    17101 tagctgatca ataattggct gtaatttcgt atttccagca cttgtttgca atttagtcag
    17161 gaactcgacc agtttatcga ttgcattttg ttgttgagat attccgtcaa taaagccgtt
    17221 caatacattg ctgatgtttt tcttttcatc atcagtcagc ttaccatctt caatagctgt
    17281 tcttacagta tctattaacg aacctgtcgt tgttgccact tgtccaattg acttcaatgt
    17341 aacatctaca ctgtttgtga tgcttggtat cgcatctttc agctgagagg cacctgattt
    17401 tgtttggttt gctagatcac ttgcctgatt ttctaaattt ttcacatctg gcaagatatc
    17461 ttgtacttgt tggatgatcg tcaagccttg ttttgcttca tcgattcctt ggttcattgt
    17521 ctcttggatc gaagcaaaat cgccgtcgat ctctgctagt tgcttaccgg cattttggat
    17581 ctctgggatt ttttcttgca aatcaaggat gatttttgct tgatctttca actcaggcat
    17641 tttgtcattc aatgcaacga ctttttcacc catctcatct actttaggga tgtaatccac
    17701 gaattcattt gctttattca gtttttcctt gatttcaggt agttgacttt gtaattcgag
    17761 gacttgtttt gtgtagccgt cgatcgtgtc caaattttca tctgttgata aaatcatatc
    17821 tttaacttta ttgatactta ctaggttcgt atcgatgtcg taacctatct cattgaatac
    17881 tttcaataaa gtcgaactgg ctgttttgat aaattcattt gtgatttgcg attggatcga
    17941 acttgctcct ttatcggtga ttttcggtgc aatcgcattg attttttcat ttaccgtata
    18001 ttcgattttt ggtttcttga tatcaccact agtaaaactc agcagatctt ctgagaaatc
    18061 ctttggcaag taaatacctg cgtaatactt tccggaacgg actccgtctt ctaattgttc
    18121 ttttgaatca acgaattgcc atcctaattg tttattttta tgaagcgatt cgatgacttg
    18181 ctccccaatg gccacttctt taccttgaaa ctctgctggc ttgtctgcac tgtaaacagc
    18241 aattggcagc tctccagtat ttccatatgg atcccataat gctttgatgt taaaccacgc
    18301 atatagcgaa gggatgatca tgatcgcgat gatcagaagt gttgcaatcg ggtttttgaa
    18361 aatccgtttc cagtcaagca aatataatcg aagcgtattt ttgattgact gcatagttct
    18421 cccccttctt cttttttcag ctgaattact ttaccatgta tttcatactt ttttcaatcg
    18481 gcttttttca aaaaagtcga atttttggac atatcacggc aattgtctca ttgtaatctc
    18541 ttacatattg agaaataatt aaggattaca gcctttactt tatcatgaaa taaaagggaa
    18601 agataatttt atgccaaagt ttttatctat tatacgtata cgcaaaaaag ctaaaacatt
    18661 ttttgtcttt gcccctacaa aaatgtttca gcttttttcg ttttttattc ctgtatttct
    18721 tatttttcga atgacaggat gattttccca gcaggaggat gtccttcaac tagctgatgt
    18781 gcttggatga catgcttaag tgaagcagac agattcattg aaataaaaac atgaaaggct
    18841 cctttttggt aggctttgat tatgccagca aaggcttctg catctagata ttcctttctt
    18901 ggaacaaacg attcataaaa gccttccttt ttttgccgca aatctagatc tgggaggtca
    18961 tttaatgcaa catacctgcc gcctggtttc atgatctgga tgccggtttc tcctttgata
    19021 cttcctttcg tcgcatcgat tacaaggtct gcctgatcag caaattgcag acctggattt
    19081 gttttatcat aagcagcaaa tgctgaagct cctaattttt ttaccttttc ctcattttta
    19141 ctggatgcac tggttaagat tcggattccc ttttcatgga gaagctggat cagacttgac
    19201 ccgaccgcac ctgatgctcc tagtatcatt acagtatctg tcggctggat ttccgtcaaa
    19261 tggttgatta gattataagc cgtaattcct ggcgtgacca ttccggctgc ttcttcccaa
    19321 ctcattttat ctggaatttt tgctactttt gctgatggca acacgacttc ttctccgtaa
    19381 gtcccgccga ctgcatggac agctactcga tcacctacat gaacagttgt gacatctgaa
    19441 ccaatctctg tcacaatacc agcgccatca ttacctggta cataagggaa tttcaatgtt
    19501 cgtatctctt tcatttctcc taaacgcaag gcaacatcat aaggattcac actgaatgct
    19561 tttatttcta cacgaacatg tccagaaccg acttctctgg gatgagcatc gatttcttca
    19621 aaaacatctt gtgcttctcc gtaaccgtta attgcaaatc ttcgcatggt caccatctcc
    19681 ctttttcttt aagtttacgt ttatgccttt attttgacaa aaaaaatgtc ttttcgtttt
    19741 ggcgcatgat tcaaaacaaa atagttgtct tttttcgcgt aacgtgttaa attatttatc
    19801 taaaagcgta aagattacga tttacgctaa taaaaaataa ttggaggaat ttgttgatgg
    19861 ctaaaaaatc aaagattgcc aaagcaaaaa aacagcaaga aacgattgaa cgctatgctg
    19921 ctttacgcca ggaattaaaa gcgcaaaaag attacgaagg cttgcgtaaa cttcctttag
    19981 atgctcgtcc agaacgattg aaaaatcgtg atttaatcga tggacgccct cgcggataca
    20041 tgcgtaaatt cgggatgtcc cgtataaaat tccgtgaatt agcacataaa ggtttgattc
    20101 caggagtaaa aaaagccagc tggtaatcca agtctggctg taatgtaaag gaggagggcg
    20161 catggaccac tccttggatc atcaaaaaag atacgttcgc ttcttctagc agaaatgctg
    20221 gtactagaag cagacgtatc tttttttacg cacagctggt ccttttttat atgtaaaggc
    20281 tgcatggtta cttcgctata tttatttagt aacaggagtc agtgcttcga tacccacact
    20341 aattttcaat gctcggtcaa cttctgccat aatgggtcga tccaaatggc agaccttttc
    20401 tttcaatctt gatttatcga tcgttctgat ctgctccaac aggataacgg agtctctttc
    20461 tattcctgtt ccagtagctt tgatcccaat gtgagtcgga agcttaggtt tagccatttt
    20521 tgccgttatg gccgcaacaa tgatcgtcgg gctaaaatgg ttacctagat tattttgaat
    20581 aaccaaaact gggcgtgttc cgccttgctc cgatcccacg actggagaaa gatcagcaaa
    20641 ataaatatct cctcttttta ccataggatc ctccttattc ttgatattct cttggtatcc
    20701 gctgtcctaa tccgcaagcc acttcatagt ggatcgtacc taattgatct gctatatcct
    20761 gcatagtgat ttctttgtct gcatttttgc cgataagcgt cacttttgtt ccgacaggaa
    20821 attcttgagg caatcggatc atcagttgat ccatacatac acgcccaatc gtttcacagt
    20881 agttcccttc taccaataga gaaaaaccct gcatctttcg tggccagcca tctgcataac
    20941 cgatcggtat cgttccgatc cactctgctt caggagtgat atatgtttct ccatagccga
    21001 ttccttcacc tgctgggagt tttttgactt gaatcaattc agaaaccagc tcaagtgctg
    21061 gctgcaatgg ataaacttcc ggtagtgcgt gcccagaggg attaagtccg tacattgcta
    21121 ctccataacg aatcatattg ccgattgttt catcatgcca taaagccgtt gcactattgc
    21181 tgacatgtac atatcgagga agttcgggaa gttttttcag cacttctata aagcgttctt
    21241 tttgcagatt ccaatatgta tcatctgcct gatctgctgt agaaaaatgg gtaaataaac
    21301 cttcccattc taatacacga ctttctttga tgaatgcagc tgcctctttt acttcttcag
    21361 gtgtacaaaa tccgatacga cccatcccag tgtctgcttt tagatgaatc gaaagaggcg
    21421 tctctagctt acttttctct aaaacagcag ctgctgcttg caaccattcc cgtgtacctg
    21481 cagttactga taaatcatga gcaacagcta cagtcagtgc gtcgtaagga accatattca
    21541 agataaggat gggctgagta aatccagctt ctctcaactc gactccttca tctaaatttg
    21601 aaacacaaaa gcccgaagct ccacctgcaa ctgctgcctc agctgtttca atagctccat
    21661 gaccgtatcc gttcgctttt actactgcaa acaattcttt tccttgcgga agtcttgcaa
    21721 cttcatttgc tacattttca gttatagctt ttttatgtat cactgctttc gttgggcggt
    21781 gccaagcgac aaccatcgtt tttccttctt tctagtcttc caaaataatt tgtgcaaaag
    21841 ctgtttcatt tgtatgagta atactgaccc atacattgcc ttcatgaggt gaacgcgtca
    21901 caacaggctg accattttca ttttttaaaa tctcgacatc ttgaaaggta acttttccga
    21961 tcccagttcc ccaagcttta gagaaagcct ctttgcaagc gtaacgcccg cctaaaaatt
    22021 ctacttggcg atgaaaaggc aaggactgga atagtttcat ttcatcagag gtcaagattc
    22081 gtgcaatgaa ttttggtttt tcttcgatca gccgagtgat cctaggaagt tcgactgcat
    22141 cgatcccaat tccttttatc atatgctttt ctcttttcaa taaatttaga acactctcat
    22201 tctaccaaat tataaccatt ttgccacaaa taagtccaca aataacaaaa atgaaagttt
    22261 ctcttaaaaa agagaaactt tcattttatg tttaatcgtt atttgaacgg attacaaatc
    22321 cgcgtttttt atcattgtga cgtttaccgc catctttttg gctgttatta tttttgctat
    22381 aaccgccttt tgatttgttt ccacgataat taccgccgtc acgacgatta cggttgtttc
    22441 cgccaccacg tttgccattt ttattgtatc cttttttctg cattggcaat gggcgttctg
    22501 gtgtgatctt cactggcact gcatcagctg gatcttttgc tgtcgttttt agcaaaagtg
    22561 caactaaatc ttgtgcagaa tattcttcta ataatttatc tgctgtttgc aggtatttat
    22621 ctagaccatt ttctgcaagt tttgtttcga tttgttcaac tgcagcacct aattgacctt
    22681 tgaatgcttc tttttcggtt ggtggacgta aagtcgtcat ccgttttttc gtcaagtttt
    22741 caatcacatg caagtagtcc atttcatttg gtgtaacgaa agtcacagac ataccgcctt
    22801 ttccggcacg gcctgtacgt ccgatacggt gaacgtagct ttctggatct tgagggatat
    22861 catagttgta cacatgagtg acacctgaaa tatctaatcc acgtgcagca acgtctgtcg
    22921 ctacaaggat atctaaatga ccgctcttaa atgaacgcaa tacgctcatt cgtttttgtt
    22981 gagacaaatc gccatggatt ccttctgcac ggtaaccgcg tgcttctaat ccgcgtgcca
    23041 attcgtctac acggcgtttt gtacgtccga agacgattgt taattcaggt gtttgaacat
    23101 caaacaagcg agtcatgata tcgaattttt cgtattcttt tgcgcggaca tagtattgat
    23161 cgatcaagtc agctgtcatt tctttcgctt tgatcttcac atgatgtgga tttttcatga
    23221 atttaacgcc aatattcttg atagctggcg gcatcgttgc tgagaaaagt aatgtttgtc
    23281 tttgatcagg aacttgtgaa atgatttttt cgatatcttc caagaagccc atattcaaca
    23341 tttcatctgc ttcatccaaa actaatgttt gaacggttcc aagtttcaat gtgtggcggt
    23401 taatgtggtc aagcatacgt cctggtgtac ccacaacgat atgtggacga tctttcaagc
    23461 cacggatctg acggccaata tctgctccgc cgtaaacggc ttgtacacgt acttttttgt
    23521 cgcgccctaa acgatataat tcttcttgtg tttggatagc taattcacgt gttggtgcga
    23581 taactaaacc ttgtaattcg tgacgatctg gatcgatttt ttctaacatt ggtaatccaa
    23641 atgcagctgt tttccctgta cctgtctgtg cttgtccaat aacgtctttt ccagctaatg
    23701 ctagtggaat cgtttctgct tggataggcg ttgcttcttc aaaaccagcg cgttcgactg
    23761 atttcaacaa ttctggtgat aattctagtt ctttaaattt caaatgaatc ctcctatatg
    23821 gttgttaact gaggatgtag cttttttgct accccgtcga tttgttcggt caaagtgtat
    23881 tgtttctttt tgacagtggc cacgcacgtt tttatccatt cttagcccct gtggcaccac
    23941 cgaaaaatca ctttgcgaag tttaggcatt agaaaagcca aacgggtcgc gttcagcttt
    24001 atcattatta tgacatctgg cttcaacaag ttgtttctat ggcaaaacca gtccaatttc
    24061 tcttcatctt tcggcaatag gttattctaa catagttttt cgttttctgc aagtttacag
    24121 atttttattt catttgctga agttgttcta ccacttcaaa taagcccatg ctgttgctgc
    24181 cttttagcat aatactatct gttgcttcaa gattttgttt gatagcatca atcaatttat
    24241 tcttttcttt ttcaaaatag aacacaggta gttgggggta tttttggttc aattcttctt
    24301 ttagtgcatg catttgttcc ccataaagga aaatcatggc gaatttctca ggatcgatat
    24361 gttcactcat ttgcgcatgc atatttaaag actctttccc taattccagc atatctgcta
    24421 acacagcgat tctgcgtcct tttgttggca gatttttaaa agtatctaat accagtccca
    24481 tggctgttgg atttgcattg tagacatcgc tcaaaatagc tgcaccattt cctgccgtta
    24541 gccattcggt tctgttttga gtgatagaaa catgggcgag cccttggcgt atctcaggta
    24601 tggacagtcc aaagaagcgc ccgatcgtat acgcaatcaa agcattcgtt acattataac
    24661 ttccaggcag cgggatcgta aatgtttctc cttctactac aaaatcggtt tgtgcttttc
    24721 cttttgctgt cacttccgca tgaacatcgc catttccaat accgaatgtt gtgactgttt
    24781 gtactaatgt tgccgttaaa ggtgtcagca acggttcatc tgcgggcaca acgagtaatc
    24841 cgtctgatac aagtccgtca gtgatttcca ttttggcttt agcgattcct tctcgcgacc
    24901 caagattttc tatgtgtgct tcaccgatca ttgtgattgc tgcagctttt ggttgagcca
    24961 taagtgaaag ttccgtgatt tcattggcgt gatccatgcc catttccaaa acgatctttt
    25021 ctgtatcttg gggcatgtgc aaaatcgtgt aaggaagacc aatattattg ttgtaatttc
    25081 cttgcgtctt atatgttttg tatttttcag acaagacaga cgcggttaga tctttcgtgg
    25141 tggttttacc attgcttcct gtaattgcaa cgacttctgc gcctactttt tccaagtaat
    25201 aggttgccaa ttgctgcatg gctttcaaag gatctgctac aggaataact ggaaaatttt
    25261 ttggagcttg ttcaacaggc aagctccaaa atgaagcaga tgctccgttt gtgatcgctt
    25321 gctggataaa ttcatgtcca tctcgcgtac cagcaagagg aacgaataaa cttccttcgg
    25381 ttatcttccg gctgtcaaat tcgacattgg taatctttgt cagtgcagag cattcgctgc
    25441 ccacggcttg tgcaatttct tgtattgtta gattcattga gatctctcct aaaaaaggtt
    25501 gtgaaaaaag cgtttggcct aaacggcggg ttcgcatgac tctcacttcc aagtaaattt
    25561 ctctattcgt atttagaagc tagaatgaac gcggttacgc aatcactcca gcctcttctt
    25621 tatttctctt tattcatttt tttcaaaaaa agattgacgc tgatggtatc gattcattcc
    25681 taactggatc agttcttcaa tcaaatcacc gtatttcaag cccatatttt cccataagag
    25741 tgggtacata ctgaactccg taaatcctgg catagagttt aattcattca ggaataattc
    25801 atttttattt gtcaaaaaga aatcgcaccg gctcaatccg cttccaccta acatcgtgta
    25861 agctaacttc gcgtactctt gcgctttttg ataaacttct tctggcactt cggctggaat
    25921 ctgcatttcg attttattat tgatatattt tgcttcataa tcatagaatg ctacgtcttt
    25981 tacgacttca ccaggcaaag tcgtccgaac gtcttcattt cctaatacag caacttcgat
    26041 ttcgcgcgct tcaattcctt gttcaacgat tgctcgagaa tcatactgat aggctgttgc
    26101 taaagcattt tgcagctctt ctcggttttc tgcttttgta atgccgacac tagaacccat
    26161 attcgccggt ttgacaaaca tcggataaag caaagaacct tcacattgat caaatacttt
    26221 tttaggattt tctttccatt gattcttaag tactggtaca taaggaactt gcggcacacc
    26281 agcagcttgt aaaatatact tggtcatgat tttatccatg gcacatgcac tggtcaatac
    26341 gcctgcgccg acataaggca tattcaatgt ctctaagaag ccttggatcg ttccatcttc
    26401 cccgtttggc ccatgtaaaa ctggaaaaac gatggctcct tcttctttga tttcgcccgg
    26461 attgatcact tttcctgtaa agccttcctc tgtctgtcca cttgggtccc atgaaagatg
    26521 caagacatct ttgctggcag gtttttctgt tagtaatgga cctttgaccc attgtccttc
    26581 ttttgtaata aatacgagtt gaacttggta ataattataa taaatggcat ttaaaactga
    26641 aaatgcggaa agaatggaca cttcatgctc tgcgctgcgt ccgccatata gtaaagtaat
    26701 cttcaaaaga ttgtcctcct gtattctaaa tcgttcagtg atttactctc atattttagc
    26761 acaaaacaaa aataaacgaa taacttattt ataaaaaaac gagccagctt ctaaaagaac
    26821 gagcgtcatt tattttttgt cagctagatt atttgtcccg ttttttataa aaagttatga
    26881 taaagaaaag gagggatgat gatggcgcaa acaaatccac gcagccgctt ggcagaaatc
    26941 attcgagtgt tcatcaatta caatgtagtg ccaaactttg tacagcaaaa aaatcccgaa
    27001 caggtaaaaa aagcctttga agaactaggc cccaccttca tcaagatagg acagatgctt
    27061 tctgttcgag aagatttgct ttcttctgcc ttcactcaaa cattcaaaac attacaagat
    27121 agtgtaccta gcgacacatt ttcaacggtc aaaaagacga tcgaaacaga actttctctt
    27181 tcattatcag atatattcga tgatttttca aaatcgcctt ttgcctctgc ttctatgggg
    27241 caggctcatc gtgccaagct gaagaacggt gacgcggtag tcgttaagat ccaacaccct
    27301 aacatcgccg aagagatccg acttgatctt caactatttg aacgagcgat ccctttgatc
    27361 aaatacattc ctgaaactag cgtagttgat ctaaaagggg tactccaaga agtaaaacgt
    27421 tccttgatca atgaaatgga cttcttaaaa gaaagtcaaa atggcgaaca attctatcaa
    27481 aagaacaatg gctggaaaga gatccgcagc cccaagattt atgacgcttt ttgttcgaaa
    27541 aaagtgatcg tgatggaaga aatgtctggt aaaaacttga atcatttaat gaacgccgaa
    27601 aacaaaactg aaacttttat cactggaatt caaaacaagc agctgaaaca agaagttgca
    27661 aagcttttag ttgagaactt tatgaaacag gtgtttgatg atggtttttt ccatgctgat
    27721 cctcatccag ggaatctgct attccatgta ctaacaaaag aagaacagac acaagctagc
    27781 cgaaaaactg aaacagtcca tgaaaaagag ttcggttcct ttgcttttcg agcctccact
    27841 tctgcagaag accctgttgc gccatatacc atcaactaca tcgattttgg tatgatgggg
    27901 catctttctg caggacttag gcaaaaactc acacaagccg ttttagcgct gtatacgaaa
    27961 gatgcttatc gaatcgaaaa agcggtcctt cgactttgtc agcaagaagg ctcgtttgat
    28021 gaaagtcggt tccaccaaga actgacaagt tttcttgaac agtattatga ttctcctatt
    28081 gatgaaatca acttgcaaga agtattcaca catgtcgtga cgatttgtca tcaaaataat
    28141 ctgcagttcg atcgtgatat cacgcttttg ctgaaagcat ttgggacatt agaaggggtg
    28201 atccgcgtcc tcgatcctga agtttccctc atggaagtcg cttcgccttt tgctcaacat
    28261 tattttctca ctcatctgga tgtagaagat acattgaaac aaagcggatt agacttattg
    28321 gaagggatga aggctgcgcc taagatccct caacagctcc accatctatt agaaatgtgg
    28381 acttcgggac aaggaaaagt caatttggaa ttgaaaaagc aagacaaatt attgagcagg
    28441 atcgaaagta tgatcaatcg gcttgttttc ggtatgattt tagctgcatt gatcgtaggg
    28501 tcctccctgc ttgtccaagc agcacctgta gaaaacgcag aggtcgtttc cttattaggg
    28561 atatttacgt atgctattgc cgcattcgtc attatttttt tggcaatcga tgcattgatc
    28621 caaatgtata agaagaggaa aaagtagaaa aaggttgtga cagaaagaat cagcttcaag
    28681 aaataagaag gggttttaca taaattgttt ctcaaatttt tgtgaaaatt cggcttattt
    28741 ttcgaagagg ctacttctga aacaccgaga ataaaaaatg tgaaaaagct gttctgcacc
    28801 cagtaataag aacaatcaaa aagggaacat ggaacacatt tatcctgttt ccctgttccc
    28861 tttttgatgt attcgagcat aaataaaata ccttcgttac ctactatctt ctgtacttgg
    28921 taaaccttag tcaattgcct tttttaacgc ttgacgaaaa gacatatgat ctttctccgt
    28981 aaagggtttt ggaccctttg tccttatgcc agcttttctt aactttccac tatgataacg
    29041 ttgttctaga agcgtgccga tattcgcttc gtcaaattct tttccagagt gatggaacac
    29101 tcgcacaccc ttcggcaata caagtgaagc taaaccataa tcttgcgtga tcaaaaagtc
    29161 attcttctta accataccga cgattttata atccgcacta tcagctccgc ggtctacgta
    29221 gacgatttgg acatgattag cgtattcttt ggtcgtataa tggtccacac tggtcacgat
    29281 aaccacgtca agaccatagg attctcctaa ttttatgaca tcttctttta ctggagaacc
    29341 atctccatca atgaatagcc tcattctttt tcgccttctt ccataaatga taaacagtta
    29401 ctaatccgct cagtaaaaga ccaacattga tccattttgc caagataagc agttgtacac
    29461 tttctatcgg cactgattca tggttcagcg tgatcaataa tagatggatt ggctctccca
    29521 tttcttttgc taaaacaaac aaaagcgtca tcaataaaaa ctggtggatc acactccctt
    29581 tattttgagt agtatacttg acaacgatcc ggcgatccat tggaatagct gtatcagttt
    29641 gataatattc cttatttagc cagctgcctg ttactacgtg ccagaaacta agcagaaaga
    29701 cacctatcgc aaaaccattt ggaatcaaga ggaccaataa agaagaactc agtccaagca
    29761 attgcatagc caatggagac catgttaaac gtttcaaaat ccatggtccg actatttttg
    29821 ctgcaatcaa gcctgctgca taaggcaaat acataaaaat cgaaagtttt tctaatccgt
    29881 acagtatgcc aacataaaaa ctgctaaata aaaaaaggaa attccctagc attccattta
    29941 gaaaagtaaa cagatttagc cagcttggca acttccattc cttcttctgg tgtcctaaaa
    30001 caaacacgaa caagatgaac agcagcaaga aaccataaat agcaagatca aaaagccttt
    30061 catcgaataa caatcgggca tttctcaaaa gaaacaataa gccaaagaat atcaaaaata
    30121 aaacaagctc tcgcgtagct accagatttc ttttgatgtc tttgaaatcc agttcgtaac
    30181 gcggataatg actaaccgta tggtaggcca tgacgtaaaa aagtgtataa atagtaaagc
    30241 ttgctgcaat ctgagtcggt gcaggcaact caatcgcttg atacaggaga aatagccaga
    30301 tagctgcaaa taagtacttt ttcccatcca tattgataaa accttgcgtc ttttcgtgaa
    30361 aattgaccgt ggtattagca ggtacaagcc atgctgcact cagccctagc aaaacacttg
    30421 aaaaataaaa taaaggaaaa taaaatacgc ctaatattcc aagcaatgaa ccagcaccac
    30481 caatcagcag agcgatcatc agcaacgtgt aactatctag accaaatcgt agactgttga
    30541 ctaaaaatag tccagtcatc cgaaaggcat aaaacagcgt aaatggcaaa atcgtcagcc
    30601 aattatgcga gtctgttagt cccaaaaata aaagataagg aataaaaata accgtgttac
    30661 ttaagataaa gggcgttgtt tgtgtaaaat tacgcccaaa tcgtttgagt ttttcgaaca
    30721 tgacaatggc tccttaaaca gtcttataac taatctgctc ttcgatttta gcattaaagg
    30781 ggaaataaac caattttaca gaatatattc ttgtaaaaaa aaatacagaa agaagcataa
    30841 gcttctttct gcatttttac ggtacttgat tactcaagca gacaagccct tctccacaaa
    30901 agtcttgccc gacacccctg catccggcag gtgataaaga tagaaatcaa ttatcctgtg
    30961 cctccaaaat caattaggag ctctgtaaac cagagtcctt ctcgacccgt cgcataaatt
    31021 tactttaaca cagttcctat gtttatgcga gagctttttt cattttataa tcgaccattt
    31081 gaaatccttg accgatatct gaaataagtt cttctgtaat ctcaaatcct tcatgcttat
    31141 aaacctcgat tgcttgatga ttcccctgat tcactcgcaa aaagatctct ctttcctttg
    31201 tttgcgcagc aagttcttca taccagcgaa aaactgaaga agtcaatcct tgaccacgta
    31261 ctttatttag caaatataat ttacttaaat aaatttttcc atttatctct tcataagcag
    31321 tgtaaccgac agtcttttct tcgcttttta ataaaaagta ggacacacct tctgtcagtt
    31381 ctttttgaat ctgttttttt gactgataag ttttcagcat gtaatccact tgtttttgac
    31441 caatgatcgg cacaaaaact tccggccata cttcttgtat gactgccatc agctcatcca
    31501 gttcattttc tgttactttt attttttcca tacacccacg tcctttttta cattataaat
    31561 gaccttgcgc ttgatggaat aaaaagagca tgatcatgct atacacaccc accgaccacg
    31621 gtttcaagaa aatcacaatc tgcataaatt ccctaaaaga taatttcgtc aaaccagcta
    31681 ctagacagat caagtcgtct ggcgcaaagg gaagcagcaa ggtaacaact agtacttttt
    31741 taatatcttt cgtttttctt gtcagcaatt catcaaattt ctgatacttt tttggcgata
    31801 aaataagctg gacaaaggct acgccatagt aacggacaag caaaaatccg attgcttccc
    31861 caatcactag gccaatacaa ctatataaaa gcccaaatcc attgccaaac atcagcatgc
    31921 ccgctactga ggagatccct cccggcaaaa taggaatgat cacttggatc acttgcaaca
    31981 gaacaaatat catcactgca tacttgccaa attgctggat aaacgcttgc aaagacgtgc
    32041 tcgatcgaaa aatcccatga tgataagcat aaagaatcaa cacaaagaaa aaaaacagac
    32101 caataatggg catgatctga agacattttt gtaaaactga tcctttttta gacatctata
    32161 ttccccacat cttttttcat ttattaaaac tgcctcctta tgaactgaac cccaaaagtt
    32221 gaactattta atggactgtt tccgatattc tactggagat agtccattta actttagttt
    32281 tatccttttg ttattatacc acctaatata ctcatttaat ttactttgaa atatctcaat
    32341 tgatcggaat ttttctcgat aaaaaaactc tgatttaagc acaccaaaaa aattttctat
    32401 tacagaatta tctaagcaat ttccttttct agacatgctt tgaataatgt tattctcttt
    32461 taattttttt tgatattgtg gcatctgata ctgccatcct tgatctgaat gtagaatcag
    32521 tgaaccctca gttccctttt ttttaattgc ttgttgaagc atttcttcaa tcagtttata
    32581 tgttggacta gttgatatac tataactaat tatttctccg ttaaataaat caagtatagg
    32641 agatagatag atttttcttc cttttatctt gaattcagtg acgtctgtca cccatttttt
    32701 attgggtgta tctactgaaa aatttcgttt taacacattc ttggcaattt ttcctactgt
    32761 tcctttataa gatttatatc gctttattcg gatttgacag gtaagtccca tttgggacat
    32821 taattttcta actgttttat gattgattgt atatcctttc atctttaacg ctaaagtgac
    32881 tctacgataa ccataagagt ttcttgattc ttttacaatc gctgtaattt cttgctttat
    32941 cttgctatat ttatctggct tatctaattt ttttacccag taataataag ttgacttcgc
    33001 taattgtgca attgaaagta atagattcaa tttaaattct tttttgagct gaaggattgt
    33061 tttaaccttg atttcttctt gctcaaatct tgttcttgaa tcaaggtttc t
//
LOCUS       DAYYGS010000026        32177 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000026 DAYYGS010000000
VERSION     DAYYGS010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.026"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..2656
                     /gene="clpB"
                     /locus_tag="ACHA44_001113"
     CDS             47..2656
                     /gene="clpB"
                     /locus_tag="ACHA44_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFM8904421.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2807..3493)
                     /locus_tag="ACHA44_001114"
     CDS             complement(2807..3493)
                     /locus_tag="ACHA44_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904422.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(3471..3965)
                     /locus_tag="ACHA44_001115"
     CDS             complement(3471..3965)
                     /locus_tag="ACHA44_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HFM8904423.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(3985..5205)
                     /locus_tag="ACHA44_001116"
     CDS             complement(3985..5205)
                     /locus_tag="ACHA44_001116"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904424.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            5352..7187
                     /gene="lepA"
                     /locus_tag="ACHA44_001117"
     CDS             5352..7187
                     /gene="lepA"
                     /locus_tag="ACHA44_001117"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFM8904425.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            7383..8294
                     /locus_tag="ACHA44_001118"
     CDS             7383..8294
                     /locus_tag="ACHA44_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904426.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            9305..9541
                     /locus_tag="ACHA44_001119"
     CDS             9305..9541
                     /locus_tag="ACHA44_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904427.1"
                     /translation="MFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTELKDKN
                     NVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            9654..9896
                     /locus_tag="ACHA44_001120"
                     /pseudo
     CDS             9654..9896
                     /locus_tag="ACHA44_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708076.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9986..10225
                     /locus_tag="ACHA44_001121"
     CDS             9986..10225
                     /locus_tag="ACHA44_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904428.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            10372..14655
                     /locus_tag="ACHA44_001122"
     CDS             10372..14655
                     /locus_tag="ACHA44_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HFM8904429.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            14754..15119
                     /locus_tag="ACHA44_001123"
     CDS             14754..15119
                     /locus_tag="ACHA44_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904430.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            15121..15573
                     /locus_tag="ACHA44_001124"
     CDS             15121..15573
                     /locus_tag="ACHA44_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904431.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            16022..17497
                     /locus_tag="ACHA44_001125"
     CDS             16022..17497
                     /locus_tag="ACHA44_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HFM8904432.1"
                     /translation="MKAHFFKYRGRRIRYFNRYLWFCYYGFFLFPFLILLGWFSWKVF
                     YPRLSTFSNWQMYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLVRML
                     IDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGHLLS
                     EVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLMKTLDWVYDQSPNMLI
                     AGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEEMII
                     CLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDMEARN
                     RVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYLMTF
                     GEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYDFTV
                     KEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            17520..17831
                     /locus_tag="ACHA44_001126"
     CDS             17520..17831
                     /locus_tag="ACHA44_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904433.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            18191..19447
                     /locus_tag="ACHA44_001127"
     CDS             18191..19447
                     /locus_tag="ACHA44_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8904434.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            19444..19989
                     /locus_tag="ACHA44_001128"
     CDS             19444..19989
                     /locus_tag="ACHA44_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904435.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            19990..20490
                     /locus_tag="ACHA44_001129"
     CDS             19990..20490
                     /locus_tag="ACHA44_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904436.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            20849..21127
                     /locus_tag="ACHA44_001130"
     CDS             20849..21127
                     /locus_tag="ACHA44_001130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904437.1"
                     /translation="MSVEHFCDCYIALLRSQQLLTVERYGAEFGYVLQAERHRATRCY
                     EKEDQVIDGYEELCIRNNRYLQQRLYEQLMMDFSNLYRGYECSMIQER"
     gene            21147..21491
                     /locus_tag="ACHA44_001131"
     CDS             21147..21491
                     /locus_tag="ACHA44_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904438.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            21510..22421
                     /locus_tag="ACHA44_001132"
     CDS             21510..22421
                     /locus_tag="ACHA44_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8904439.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVSAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            22500..23492
                     /locus_tag="ACHA44_001133"
     CDS             22500..23492
                     /locus_tag="ACHA44_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8904440.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            23489..23707
                     /locus_tag="ACHA44_001134"
     CDS             23489..23707
                     /locus_tag="ACHA44_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpD family membrane protein"
                     /protein_id="HFM8904441.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            23720..24130
                     /locus_tag="ACHA44_001135"
     CDS             23720..24130
                     /locus_tag="ACHA44_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpE family conjugal transfer membrane protein"
                     /protein_id="HFM8904442.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            24182..26710
                     /locus_tag="ACHA44_001136"
     CDS             24182..26710
                     /locus_tag="ACHA44_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8904443.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            26726..28936
                     /locus_tag="ACHA44_001137"
     CDS             26726..28936
                     /locus_tag="ACHA44_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8904444.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            28944..30035
                     /locus_tag="ACHA44_001138"
     CDS             28944..30035
                     /locus_tag="ACHA44_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8904445.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            30056..30598
                     /locus_tag="ACHA44_001139"
     CDS             30056..30598
                     /locus_tag="ACHA44_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF0163 family protein"
                     /protein_id="HFM8904446.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VGQEAQKIDAIKIYYVRSAY"
     gene            30793..31050
                     /locus_tag="ACHA44_001140"
     CDS             30793..31050
                     /locus_tag="ACHA44_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904447.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            31064..31396
                     /locus_tag="ACHA44_001141"
     CDS             31064..31396
                     /locus_tag="ACHA44_001141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904448.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            31441..31989
                     /locus_tag="ACHA44_001142"
     CDS             31441..31989
                     /locus_tag="ACHA44_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFM8904449.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            31996..>32177
                     /locus_tag="ACHA44_001143"
     CDS             31996..>32177
                     /locus_tag="ACHA44_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904450.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLP"
BASE COUNT        10986 a         4988 c         6847 g         9356 t
ORIGIN      
        1 tcaaagaaat aagtgatctt acctttaatt aggagggtat acctgtatga atattgagaa
       61 aatgactacg acgcttcaag aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa
      121 acatcaagat atcgacattg cccatgtatg gaaaatcttt ttacaatcta atcattttgg
      181 tcgaaatttc tatacggatg ccggattgga tgtcgaatct tttgaacatg aaattgatcg
      241 tctactggat gagtatccag ttgtttctgg tggaaacgtc caatatggac aaaacctcag
      301 ccagaatttc tttcgattat tgaatgaagc agaccaaata cgtgaaagct tcggcgacga
      361 gtttctttca acggaagtcg ttattttagc gttgatgaaa ttaaaaaatt atccgttaac
      421 tgtttatttg aataaaaacg gattatctga aaaagaattg cgtaaaaaca tcgaagagat
      481 gagaggagga gaaagagtga cctcacaaaa ccaagaagaa caatacaaag cattagaaaa
      541 atacggcgtt gatttagtac agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg
      601 tgatgaagaa atccgtgatg tcatccgtat tctttcacga aaaacaaaaa ataatcctgt
      661 tctgatagga gaacccggtg tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat
      721 cgtacgcaaa gatgtgcctg aaaatttaaa agataaaacg attttttcat tagatatggg
      781 tgcattgatc gctggagcaa aattccgtgg tgaatttgaa gaaagactaa aagcagtctt
      841 gaaagaagtc aaaaaaagtg atggacgtat cctcttgttc atcgatgaaa tccacaatat
      901 cgtaggggca ggaaaaacag aaggaagtat ggatgccgga aatctgttga aaccaatgct
      961 tgctcgcggt gaattacatt tgatcggtgc cacaacttta gatgagtacc gtcaatatat
     1021 ggaaaaagac aaagcgttgg aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt
     1081 ggaagatacg atcagtattc tgcgtggatt aaaagaacgt tttgagatcc accatggtgt
     1141 taacatccat gacaacgcat tagtagcagc agctacttta tctgaccgtt atattactga
     1201 tcggttctta ccggacaaag caatcgattt gatcgatgaa gccagtgcaa cgattcgggt
     1261 agaaatgaac tctatgccaa cggaattaga tcaagttact agacgtttga tgcagttgga
     1321 aatcgaagaa gctgcactga aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa
     1381 tttgcaagaa gaattagctg aacttcgtga agaagcaaat gcgatgaaga tgcagtggga
     1441 aacagaaaaa gaagaagtca attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa
     1501 acacgaatta gaagatgcag aaaataacta tgatttagaa cgcgctgctg tattgcgtca
     1561 tggtacgata cctcaattag aaaaagaatt gaaggaatta gaagctaaag caaaagacag
     1621 cgagattaag atggttcaag agtccgttac cgaaaatgag attgctcaag tcgttggcag
     1681 attaacgggt atccccgtaa cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt
     1741 gaatgaaaca cttcataaac gagtgattgg tcaagatgaa gcagttgatg cggtcagtga
     1801 cgcggtaatt cgttcacggg ctggattgca agacccaaat cgtcctctag gttctttcct
     1861 tttcctagga ccaactggtg tcggaaaaac cgaattagcg aaagcgttag ctgagaactt
     1921 gtttgactca gaagatcata tggtacggat cgatatgagt gaatatatgg aaaaacatgc
     1981 tgtgtctcgt ctagtcggag ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt
     2041 aacggaagct gtcagacgaa atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc
     2101 gcatcccgat gtcttcaata tcttgttaca agtcctagat gacggacgct tgactgattc
     2161 aaaagggcga gtagtagatt tcaaaaatac ggttctgatc atgaccagca atatcggttc
     2221 acagttactg ctggaaggcg tgactgctga tggaacgatt ccagaagcag tggctgaaca
     2281 agtcaacacg ctattacgtg gaaacttcaa accagaattc ttgaaccgaa tcgacgacac
     2341 gattttattt acaccattga gcctagacaa cgtgaaagga atcgttgaca aaatggtggc
     2401 gcaattggca caacgtctgg aacatcaaga aatcctgctg acaatcagtg atgaagccaa
     2461 aacgtggatt gctgaaaatg cttatgaacc agcatatggt gcaagaccat tgaaacgatt
     2521 tatcactaag gaagtagaaa caccactagc taaagaaatc gttgcaggac atgttatgcc
     2581 taaatcaaaa gtaacgatta ctttacttga tggacaactt catttcaaaa cagaagagct
     2641 agaagaaatc gtctaatctt ccattgagac tataaaagaa aaaaggggtc tgacacaact
     2701 agtgtcagac ctcctttttt caaataaaca gtgttccaga agtagtttct tcgaaaaata
     2761 agccgaaatt tcttgaagct gcccgcttct gtcacaacct cttttattag gtatttttca
     2821 ctttttctaa tttataactt tcaaagtaac gctcatcttt tttactttga gaccatgcaa
     2881 acaatcccat taaggcagca cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca
     2941 atgctgctcg tccgacaaac agtgtgccat cagaagcagc aaaacgttga agattcatgc
     3001 ctaagaactg gtcgcataag aattcccaga actcccaaaa cacgccgcaa agtccagcaa
     3061 acgcaaagcc gaataagagg aacaaccaag gactggtttt tgatatctca gcatctttca
     3121 tcaagtaacc aatcaatcca tagccaagag ctgtcaggac cattggactc accgcatgta
     3181 agattttgtc ccaaaaagag atgatactga tcaaatgcat ccctgtgcct aaaaagacag
     3241 agatgaacaa gaaaaaccag taaaacaaaa cgattgctgc tggtatttta agtttgaaaa
     3301 ctttcaaaat gacttgtggc atgaaaatca atgcaatccc taataaagat tccacgataa
     3361 atgtgattcc ttgagaagtc gaatattttt gtgtgataaa ttcataaaaa ttatagcaga
     3421 caacgaatac accaaaagca agtaaagcta tttgcacctt tttgatttct ttaatcactg
     3481 aaagtccctc catctaaaaa tctttttact tctgaaagca actcaatttc tgaagaaacc
     3541 acttttccgt tcaatttgat caaacctatt gtatataagt tcaggtagtg aaattgattt
     3601 tcagctgttt cttgtagtgc agcgattttt ttcgggtggg ttccgccttg ctgtcgagta
     3661 tcagtatata agccgatcac tgggatgcct ttcgcataag ccacaccgat ttccgatgct
     3721 accccggcat cgatcgtcag cccatctagt aaagcaatca ttaaatcact agccaacact
     3781 ttttctgtgt cagcaagagc aatcattcga ctgtctgcat aagcagtttt gtcattgatt
     3841 gcttcatttt cttgaggaag atagatcgaa agctcagaag agagctctct gatttcttta
     3901 actaactgtt ggttatataa caaatcactt ttagcaaaca ttggtgcggc aaaataaatt
     3961 ttcatctaaa catcatcctt tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg
     4021 aatgatacgc tctagtgact tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc
     4081 caaataagaa gcacggcgat aattcatcat caagcaagtc aatgtttgga tatccagtcg
     4141 aacggatttt cctataggtt catttaagac acggacttgt ccctgttcat cccatgttaa
     4201 agcgaagaca ccattattcc aaggagcgac aggatcttca atgatgaaat gaaaagcatc
     4261 agctgttcca acaaacggaa accgttgaag gaattctttt acatcaacga tccgagccat
     4321 aaaatatggt tcgatctgct ctttgatctg actatcttca atcaaaaatg ctaaaggctc
     4381 atttttataa atatctccat gtacccagta gatcatggaa aaatgtgcgg aaataaagtt
     4441 ccataagcca tttcgagctt cttgatcaag atagaacatt tcttttacgt gaaatatttc
     4501 ttcagctacc cagtagaaca gaacacctct tggttcatgg ttgctatcgt agtagacagc
     4561 ggctgtgcgc tcatcttcat tttcgaaacg ccaatactct tcccaatgaa aatcactgcg
     4621 gaataaagca ccatggtttt gttgggcaaa tttgtcataa acggaaaata cgtctggatg
     4681 atcaactggt aatcgctcaa ccataccact tactgggatt tgtttaggta gctgtgtatc
     4741 tctgatttta aaggaaagtt tatcagacat gatttcccat cctttacgcc gatagtaggg
     4801 gatactgtaa ggatacaaat aagagatcca ttgcttattc ttgcgcatat tatccaatgc
     4861 aacgataatt agatcttgca tcaatccatg gttagcatat tctggataag ttccaactcc
     4921 agtcacacca cccattttat aacgtgcacc gtgaatattg acttcacaag gatagatagc
     4981 gatttgagag atcagtttat tttcgtggaa ccatccgaac actttcgata cttccaaaat
     5041 cggttttttg gaacggataa aagctcgttt attctcaaac ccgctttcct cgatgtctgc
     5101 ttcagtcact tggaacacat aggaaagcaa ttcattgaat tgatcgatat gttcttgagt
     5161 gaccggtttt aattcaagat tttctctaaa atctttttga tccatttttt catctccatt
     5221 tattagcttg ataacagtat agcaaaattc tacaaaaaaa acttctatag ctgactaatt
     5281 aaaaactctt taaattactg cttttcgttg gtataatgga aagtgctgat tatatgaaaa
     5341 gaggagacgc tatggatata aagaaaatga aagaacgcca agagcagatt cgtaattttt
     5401 cgatcatcgc tcatatcgac catgggaaat ctacattggc cgatcgtatt ttagaaatga
     5461 cgcacactgt gacgtctcgc gaaatgcaag atcagctgtt agactcgatg gatttggaac
     5521 gtgaacgtgg aatcacgatt aaattaaatg ctgtagaact caattacaca gcgaaaaatg
     5581 gagaaacata tatctttcac ttgattgaca ccccagggca cgtcgacttc acctatgaag
     5641 tctctcgaag ccttgctgcc tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa
     5701 tcgaagcaca aacattggcc aatgtttatt tagcgttaga caatgatttg gagatcctgc
     5761 cagtcatcaa taaaatcgac ctacccgcag cagatcccga acgtgtccgt caagaaatcg
     5821 aagacgtaat cggaatcgat gctagtgaag ctgtattagc cagtgccaaa tcagggattg
     5881 ggattgaaga tattctcgag cagatcgtgg aatatgttcc tgcaccaagt ggagacttag
     5941 atgcaccgct aaaagctttg atctttgact ctatttatga tagttaccgc ggtgttgttc
     6001 taaatgtccg gatcacggac ggtatggtca aacccggaga caaaatcaaa ctgatgagta
     6061 atggcaaaac atttgatgtt acagaggtcg gggtcttctc gccaaaagca gttgctagag
     6121 actttttgat ggtcggtgat gtcggttata tcacagcaag tatcaaaact gtccaagata
     6181 cacgagtagg ggatacagta acgttagcag acaatcctgc cgctgaagct ttaccaggtt
     6241 accgaaaaat gaatccgatg gtttattgtg gtctttatcc aattgatact tctcgttaca
     6301 acgatttgcg ggaagcttta gaaaaactgc aattgaatga tgctgcttta caattcgaac
     6361 cagaaacctc tcaagcatta ggttttgggt tccgctgtgg attcctcgga cttcttcata
     6421 tggatgtcgt ccaagaacgc ttggaacggg aattcaatct agaattgatc acgactgcac
     6481 catccgttat ttaccatgtt aataaaacgg atggtacgac tgctacagta gataacccgg
     6541 cagattttcc tgaacctgta acgattcagg atgtagaaga accattcgtc aaagcacaga
     6601 tcatggtccc aaatgatttt gtcggtgcag taatggaact ttctcaacga aaacggggag
     6661 aattcattac gatggattat ttagatgatt accgagtaaa tgtcgtctat aatattccgc
     6721 tttctgaaat tgtctttgac tttttcgaca aattgaaatc aagcacgaaa ggctatgcct
     6781 cattggatta tgaaatgtct ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc
     6841 tgaatggaga aaaagtcgat gcattaagct ttatcgttca ccgagacttt gcctatgaac
     6901 gtggaaaagc aatcgttgaa aaactgaaaa aactaatccc acgccaacaa tttgaggtgc
     6961 caatccaagc agctattgga caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta
     7021 aaaacgtctt ggctaaatgt tatggtggag atgtttctcg taaacgtaaa ctcttagaga
     7081 aacaaaaaga agggaagaag cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag
     7141 ccttcatggc cgttctgaaa atggatgagc aagatagtgt gaaataactt ataatcaatc
     7201 gaaaaaccta ttaaataaac ctttttgagt agatgcaaca ctgaggatta tctgtataaa
     7261 agattttaaa ctgtcatttg gttatcacgg cgatactaaa attaacaaaa ttggaatatt
     7321 tttcttgaag atttttagct gtcagtatat gatgaaagca tctcatagga ggtgcttttt
     7381 tcatggttga taattcaagt ggaagggttc ttaaatctct aagaaaagag aaaaaactat
     7441 ctcaaaaaaa gttagctgat ttagcaggta tttcacaaag tactttagtt aaatatgaaa
     7501 aagggtcaag aaaaatccct aaagacgtag ataatactct aagcaagatt ctgaatatag
     7561 aaactctaat aaaagatgag gaagataaga ttgagattct catcggtaaa ctaattgcat
     7621 acagagatat gaataagtta cttaacaaag agttagctga taatatagga attagtgaag
     7681 tattactcag ttatgtgtta aatagaaaga gaaatccgtc aaaagaaatg caaaaaaaaa
     7741 tcgatatttt tctattaagt aatgaaaaag aaattttgaa ggagattaat cgtgatagtg
     7801 agatatttag tttatcaaaa gacgataaaa ttgttatggg aaaaagaata cgagaagtcc
     7861 gaaagaaccg ggaagagacg cttgaaaaat ttggtaaaaa ctttaccatc tatacaggca
     7921 aaaatgttat aagtagatgg gaaaaaggta ttaatattcc agatattgaa aaactaatga
     7981 atattgccta tcttggtaag gtcactgttc cttaccttat gtacggagaa gattataaga
     8041 atatacttcc aaaagatgaa agagttagcg attttaaaaa gataaattct tttagtatgg
     8101 gattaaggat gaggaaaatt aggaaagatt attatctaga aagggaggag tttggaaagc
     8161 ttttttctcc ttcaatttca aaatggtcca tagataggta tgagaatggt agagatatac
     8221 ccaatactaa tcgaattatc caatatgctt atataggaaa tctttcttta gaatttttga
     8281 tttacgggat ataaaaatag tttacgtcga tacttattaa gttatcgacg ttttttatta
     8341 tagaaaaaat ttataaaagg aggtttcttg atatgataag aaacaaaaag ggtttgttga
     8401 aagttgcttt actggcagct tttttctaag taactttttg acaagctcaa tggcaacaca
     8461 agcggaagaa gtaatacaag aactaactgt tatgaataac agctagataa aatgggctat
     8521 gtatgttatc caacaattta gtaatcacta gaagcctagc gaaagttcgc ttgataagtt
     8581 atagatgagt attgatttgt agtgtttcca aatcaaaagg gtacagagcc acatgaaata
     8641 tgttgtcctc tgcagcgtca ggatgggaat ccataccatg gagattcgga gatctgtgag
     8701 ggaatactta cgtggttaat atgtcagttg tgttggacgt agtaatggtc atagtgggta
     8761 gattgactga attcttatat gagaaaatga aaccaacaag aagatagggt tatttataca
     8821 gaattgggga tcttttctgg aaaagtaatc ggataatgcg agattcgtac ggtctttttt
     8881 aaatttctct aaaaaaaacg gcatcgaatg ggttgggtga ttgataagca atgaatcata
     8941 gactgatata gaataaaaaa tgattctatt tatcttttta catctatcgt tttttcaagt
     9001 agccgcaaat tatatcagca ttcaacaatt tttgtataca agttctagta aaaggatctg
     9061 aagaaaaagt tggttgttgt gaagataaaa aaagaatcag gttttactga gtttttcttc
     9121 aaaatttgtg ttattaggtt aggacaaaga taagaagaag tatgaagatt taaaaaagga
     9181 atgaggaaaa gatagtaatt tccgattatt ggagtttttt tatttataaa gaagaagcga
     9241 ataagttgtg attcctagat ttcgcgcgtg attaattaat gagcaaaaaa tgcggaaagt
     9301 tttagtgttt aatacacgag gacaaagttt cttaggtatt gaaggcacac gtgtaacggt
     9361 tgatgggtgg tgtgattgtt tcgaggttgg aaaacatgtt gaacttatgg catttactga
     9421 gctaaaagat aagaacaacg tggaattttt cgagagtgat attggttggg acgatcatga
     9481 agaagtttat ggaaaattta ttttgcggaa gggaaattct tatacgaatg gggaagcata
     9541 agtagcgatt tgttagagat cattgatagg attgaaattg tcgggaatat ttatgagaat
     9601 ctagagttat ttgaggtgat agaatgagtg atggttttga acaagtatca ataattaaaa
     9661 atcaagttcg ggagattctt agaaaaaaag gctatttggt agatagttat tttgaaggag
     9721 attacgaaac atgggtaggt gtctatgctc gtccagagaa taaacctacc tatttagatc
     9781 ctacgacgtc agaagatgcc tatttacaaa ataggtatcg agtagatggt tttaagcaag
     9841 actttgcgga gtggtttgaa tgggaaattg aaaacggaga agtaaaagaa gaataagagt
     9901 acttgttgta tcaaaataag ccagtagaac aaccaaacca gtagctagta atgacaataa
     9961 tactgaggaa tcggatcatt atggtatgat tgatatttct cttccagaag atcttaagcc
    10021 agatcctgta acaattggca atagtgtcat gatttttgat tcattagatg aagctatgtc
    10081 atatggttgg aaagaaatta aggatgaagg tagtaattgg tatggcatga gatttggtga
    10141 tggtgaagtt gccaaaaaag ctggtattcc aattgggaaa tggacagttg atttccacga
    10201 agcaggttct ttttttgttc tgtaattata gtgggagggt aaaatcattg atgaaatgtg
    10261 ttcagataag agcgccagat aaaaatagaa gagacagttc acgtcgttat cacttttggt
    10321 aacggcgttt tgttttatta tataaaattc tcaggaaaag gaggaattct tgtgtatgca
    10381 aaaacgaaaa tgaattggtt gaaagcactc gcattggtgg ctttttctca attctgtttc
    10441 ttggtgggaa ctgctcaagc acatgcagaa gaaacgacgg ctatacaacc tacgatggaa
    10501 gccattgccc aagtcgcttc tgaagtgaca gaagtccaac cagcagaagc cggggtccca
    10561 gctggtgaag ccactgttga acaggcccct gtgacagaag cgccaacggt caattccaaa
    10621 acgactctcg atcagacgat tggggaaaca caacacccag ggaatagtcc tggtacttca
    10681 caaccagttg aagaaactgc ctcaattaca acccctgctt cagaaacgtc cactctagaa
    10741 ggagaaaaag ctgccgaagc aacgccttct gtgcagccag agtcttctga gaaacaagcg
    10801 gaagaaaaga aacgaataga agaaacagtg gaacaaccag cggctactgc tgacaaagaa
    10861 ggaaaaaatg aggaaaacgt tgaaaaaaca atctcttcaa ccgcagctag tcaaaatcaa
    10921 ccaacgaaac caaacaaaga atctacggct agtattgaaa atcaaaagaa aaaagagcag
    10981 aacattccaa aaacaggtac acctacactg ttagtaacag taccaaccaa tcgttcggtt
    11041 cgtgttcgcc gtgcaaccaa tgcagctgtt ttaggtggtc ggaaaattga ttggggcgaa
    11101 aatattggtg ttacttctag tccgaacaat ctaaacttca ctttaaatta tatggttcgt
    11161 tttgagatta atggacaagt ggcgttttgt attgagcctg gtgtctcagc cgcagaaggt
    11221 caagtttata cagctactgc tttgaaaaac tatttgaaaa attcaaatgt acggaaaaaa
    11281 acaagcttga tctcttattt aggatatgtc acaaataaag acaaatccga tgaacaatac
    11341 attgcggcac aatttatgat ttgggaagct ttgggcacaa aaatcccaat caaaaatatc
    11401 cgtgttgatt atgatcgtcg aaaagcagaa attcaacgtt tgattaataa ctttggtgaa
    11461 aaagcaactt ttaataatca aacacatacg gtaaaaattg gcgaaacgtt gaaagtaaag
    11521 aataaaaatg attttttgaa atatgtgaaa gacattcaaa aaccaaaagg tgtcaatgta
    11581 tctattcacg gcaatgaagt atggattaca gtcacaaaag aggcaccgga taaagcaaaa
    11641 attcaattga ataaaattaa agttcaaggt gcgccattag cctatcgtcg tccaggagct
    11701 caaacaattg gtgtgttaca tccaacagaa cctaatgcgt ctgttattaa tttacaaatt
    11761 ctaaaacaag gaaacattcg tgtgtttaaa gaagatgcgg aaactggtgt gaaagcacaa
    11821 ggtgcagctt ctttagaaaa tgcggtctat ggtttgtatc aagcagatgg gaaaaagatt
    11881 aaagaaatta ccttgaaaaa aattaacggc aaagtacagg cagaaattaa agatcttgat
    11941 cctggcaaat atgttttaaa agaaatcaaa gcgccaaaag gttatgtgct ttctgatgaa
    12001 gcaattgaaa ttgttgttga acctggaaaa actgtcaatg cgaccgcaaa agatcaagtg
    12061 atcaaaggaa ccattgattt agtgaaagtt gctaataaag atttagtcga ttcaacgaat
    12121 ccagataaca aaccaaaatt agctggcatt gaaatcactt tgacgtcaaa aacaactggc
    12181 aaagtggtga aaaccgttgt gacagacaat gatggttatg ctacttttgg taaaaatacc
    12241 gttgtctttg atacgtacat tctttctgaa accaaaggaa aagaagggta taaactattt
    12301 gaaccacttg aagtcacaat ttctgaaaat gggcaaactt tccattatgt tttagaagat
    12361 aaagtaattg aacaacgtct taaagtggtg aaagtggatc aagaaacagg aaaagtgatt
    12421 ccacttgcta atacagaatt caagattttt gatactttag ccaatcgtta tgtcacaatg
    12481 gagattccaa atgatacaga aacaacggat atctttaaaa ccaatgaaaa aggctttttc
    12541 ctaactaacg gtacacttac ttatggaaaa gatcgctatc gtttggaaga aatcaaagcc
    12601 cccgtaaatt acgttttgaa tcaaaaccca ttagtgttct cgattaccaa tgatacagaa
    12661 ccgatcaaag tgatccattt tgcgaaccgt caagcacgga aaagtgtaaa attattcaag
    12721 tatgaagaat ggaacaagaa gaaaacgccg ctcgcaggtg tgaactttac cttgtataac
    12781 ggtaaaaatc aagcaattgg tgaatatacc actgatcaaa acggggaaat tttagtgaaa
    12841 ggtctagttg ccgatgacta ttacttcttg gaaaatgccc cattagaaag tttccaacca
    12901 aatacagata aacaagcatt cacggttgca ttcaaacaaa atgcgccaga tgatggggaa
    12961 acgttagtgg tttctgtacg taattacttg attccaccaa aactagaaac acaagcgtca
    13021 aataaagccg atggtgaaaa agtggttgat ccattagaaa cgatcgtcat taaagacagt
    13081 gtttcttata ccaacttgtt tgtcggaaaa gaatacacca tttcaggagt attgatgaac
    13141 aaggcaacga atgcgccaat tttacaagat ggcaaagaaa tcacaagtac cttggccttt
    13201 attgcagaca aagtcaatgg cacaaaagac cttgaatttg tggttaatgc tagtgttctg
    13261 aaaggtcaaa cgatcgttgt ttttgaaaaa cttttccgtg atggcattga agttgcagca
    13321 catgcggata tcaacgatga agcccaaagt gtgaaagtga ataatccaac cgttcatacc
    13381 acggctgtct ttgaagacgg attgacagtc gcagacccaa caggattgtt taccttaaac
    13441 gataccgttg aaattaccga gattcttcca ggaaaagcat acttggtcaa agggatctta
    13501 atggataaag caaccggtga acccttgctt gtgcatggaa atacggtaga aacgagtgtt
    13561 ggctttattg ccaaagcaga aaatgaaacg gtgatcgtac catttacctt tgatttgaca
    13621 ggtataaatg gtcgtgaaat cgtcgtgttt gaaagtcttt ttagaaatga tgaagaacta
    13681 gcaaaccata aagacatcaa cgacaaaggg caaacggtac gagtaactaa cccaagtgta
    13741 cgtacaacgg ctacacataa acccactggc ttgaaagagg ttaatccatt aacgaaagtc
    13801 accattacag acgctgtgaa ataccacgat ttggtcgttg gaaaaacata cacagtcaac
    13861 ggtgttctaa tggacaaagt aacggaaaaa ccagtgctag tagatggaaa agaagtcaca
    13921 agtaccttaa cgtttgtggc agaaacaacg gatggtacag tcaatctaga ctttattcta
    13981 gacgcacgtg cgttgcgagg caaagaagtc gtggtctttg aagatctgtt tagagaaggc
    14041 gtattattgg cttctcacgc agatattacc gacaatgacc aaaccatcaa aatcagtaat
    14101 ccaaccatta cgacgaaagc gacgaataaa aatggcggaa aagagatctt ggccttgcca
    14161 aatcaaactg tgcttgaatg ggtcaaagta gacggtcttg cgattggtca aatctatcgc
    14221 ttaattgttc aaggctactt aacaccagac ggcacaccat ttgaaggcac tcatgcggaa
    14281 aaagtctttg cggccgataa agaaatgatg gaattcttat ttgaattctt agtggacggt
    14341 cgcaatcttg caggaaaagg cttgagtttt gcggaatggc tccaagcgaa aacggaagat
    14401 gagaaagaaa cgtttgaaga agtggcgaaa cacaatgtag atctaacaaa caaagatcaa
    14461 acggttcaaa ttgtggaagc gccaaaacca ccgcaaccag tgaaaccaga acaacgggtt
    14521 aaaccagcta ctgttgtacc aatgttgcca aaaacaggtg aacaaacaag tagtatactt
    14581 atggttcttg gattagtagt attagtcatt gctttaggta taacacttta taaagtacgt
    14641 aaagccaaag attagtcatg aggaacaagc ggttaggatt tttaaccgct tgtttgattg
    14701 taatgtagaa atatttagaa tttattttgg tttttaagag gaggaaaaac tttatggcag
    14761 aaaaagcatt gcgtatgaag aaagagtttt taggtcaagt atttttattg ggttcacggg
    14821 aacctaatcc accaaaggaa attaatggtg tgtctgggcg atatgttcaa ttacaagcag
    14881 agtgttcgcg agaagaacag cctgcttttt ttgtttatct cacagctgaa gaggataaaa
    14941 gattacgatt accagaaaaa gaggtgcgta tttcatttga agaagcttat tttgtttttc
    15001 gtaaagaacg agtgaatagt cgtgaaacaa atgatatggg acaaacagag cgtcgaacaa
    15061 ctggctatca agatgttggt gtctttttaa ctggtgtaaa aattggggag gttttgtaag
    15121 atgggattaa aatttccaaa taattatgac attacagaag cattagatga aacttgttta
    15181 ggagccgtac gctttggcgc attagaagag tctgatcgtg tatttgataa tgaaacaggc
    15241 gattatacgg gagaaattcg atcacagcga gcacaattag tttttggaaa tccagatgaa
    15301 ccaaagattg cacgacgagt taaatttcca gcagatgtag atttgactgg ttttaaacga
    15361 cgtgatcctg tgaaattaat tgatccagaa tatcatgtgg tatatattcc agcacaagat
    15421 ggcaatcgtg aatattcaga tttagtgatt tatgcgaaag gattagagaa agtagaagct
    15481 ccaacaaata atcctaatat gaaacaatca caagaaaaag gtttagatca aaaacaacca
    15541 agtgttgatt ctaaggaaaa agagaaaaaa taaggttttt ttgaaagaat aggaaggagt
    15601 cttcctattc tttttatcta agatagaagg aaggggagat tttgaattca gataaaacca
    15661 atcaaatact ttgagagatt gtgcttgtta tcaaaatatc caacaattga ttgagtattt
    15721 tggagacaat ccaacgattt ttgtacaaac aaatgcatat atttgtgcgg ttattgggca
    15781 gtgtatgttt ttacgtgatc ttgttcgttt ttcattaccg taccagtgga aacaagatca
    15841 tcaggaagtg atttggattc gtatagtacg aatatgtaat atgtgtgcac acccttataa
    15901 aaatcgatta aatctttttc gattatgttt ttttactaaa tgtgatattc ctgtattagt
    15961 tgctttttgt caaaagtttt tagcacagga aaagtaaaaa cagaaaggag gaatgtgaca
    16021 attgaaagct cattttttca agtatcgtgg tcggcgaatt cgctatttca atcgttattt
    16081 atggttttgc tattatggtt tctttttgtt tccatttttg attctgttag gttggtttag
    16141 ttggaaagta ttttatccac gtttaagtac gttttcgaat tggcaaatgt atcttattcc
    16201 tggaataagt attgtgttgt atttgttgct ggttagtggg ttaattttag ggttgatgca
    16261 ttggtcgcga ctaaaagaag gatattttgc ctctgtttat tggcgtcagt tacttgttcg
    16321 aatgttaatt gacaatcggt ttgtttatac aaagaaacaa gttagtgaga aacaaacaag
    16381 ggaaaaaatg agattaccaa aagtttattt ttatcaaaag aaacaagaat tagtggtttc
    16441 ttttccttta gatggtgggc gttttcacga acgattttta aaaatgggtc atttgttatc
    16501 tgaagtattt ttgcgtgatt taattcgtga agaacgagaa aaagcccgaa tcaattatgt
    16561 attcctttca gattctggtc ggattcctat tgatcaatgt atagcagaaa atggacgtat
    16621 tcatttaatg aaaactttgg attgggtata tgatcaatca ccaaatatgt tgattgctgg
    16681 tgcaatcggt ggtgggaaaa cctatttatt gtattcttta attcaagctt gtttaagtgt
    16741 tgggacagta gatatttgcg atggaaaagc ggcagattta gcagcattag gtgacgtcga
    16801 tgtattcaaa ggacatgtgt tttacaaaat aaatgaagaa atgattattt gtttacgaaa
    16861 tgcattaaaa gaaatgaatc aacggtatgc ctatatgaaa accatgaaag atcctaaata
    16921 tgaaccaggg aaaaattatg cttattatgg gttaccaccg cattttatct tttttgatga
    16981 atgggcagct ttttatggtt cgttggatat ggaagcgaga aatagagtcg ataaattgat
    17041 tcagccatta gtattaaagg gtcgtcaagc agggatgtac attattttag caatgcaacg
    17101 accagatgca gaatattttc caagtggtgt tcgggataat ttaacattcc gtatttctgt
    17161 tggtcgattg tctcccattg gttatttaat gacttttgga gaagatttta aaaataagcc
    17221 attttacaac aaagatcaac ctggtcgtgg atatgctttt ccaggatttg atgtacctgt
    17281 agagtttttt gcgccatttg tttacaaaga ttatcacttt atcaaagaat ttgagaaatt
    17341 tactgaaatg attgtctatg attttacagt aaaagaggat agtcaagatc aaacgatggg
    17401 agatgataat cagttgttac aagaacaact agagcgccaa atgtatttag caaaagatcc
    17461 aaaaggaaca agtttttctg atgcgcatat tttgtaatca actaaggagg tgaagttaaa
    17521 tggcagagag ttgttattat agagtagcta cagcaatacg aatgattaat ccttctcttt
    17581 cttccagaac gttttatgat tggttgaatc gtattgaaca agtaactgat taccgctttt
    17641 tgcgcaaaga acgtgttttt acaggaaaag ttattaatca agtattgcta acgaaaaaag
    17701 atatagaacg attgactcga ttgtatcatt atcgagtaga tttagaagag gatttaactt
    17761 tgtcaattta tcgcgttttt tctcctgaaa aatatagtga aataacaaaa ttagatcatt
    17821 taattttgta gttattatct atcgggaggg ctctagacgt agtctggtag ccgcactctg
    17881 cctggcggtg cgcattaagg ctgcgaagca tgggcctata atgcgcactc gctagcagcg
    17941 tgtgcagctc acaaagaggt cgtgcgcttg cgcacggcct ctttgtgtaa agccccccga
    18001 tatataatat ggggggcaga aataacataa cacatagaaa aaagttttat aaaccctttt
    18061 gtatcaagtg attatctaag gtcagcagtt gtactacttt tcccaaaaaa cgagggggac
    18121 tagttgatat atcggtgttt ctaaagggtt aaaaaaatcg attttgttat tttataaaag
    18181 gagcgagaaa atgacaccga ttgagctaaa aagctacaga aagaagttaa gtgcaattca
    18241 taatataagt ggagataaaa agccgctatc acaagcaaga tttgcgcaat tgatgggctg
    18301 ctcaaaagat tacattagaa agatggaaca agggaaaaga aaaatcccag aggattttga
    18361 acaacgaatc aaggaacgct ttcgattgta tatgaaaagc ggaatagatc ttcgagcaat
    18421 gattgattat ttgcgtcttt ccttttttgg gacagactac aaaacagtga ttcaacgtgt
    18481 attacgaatg gatctgacag aatttgaaac acgtccaacg ggcttgtatc tttttgactg
    18541 tctttctgtt cgtgggaata tctgggtgtt ctggcattca aaacgaactg taacagaaaa
    18601 tgtacttgtg caattttcag gacaaggttg ccgggaatat gaggaagtca tgcgtgaaca
    18661 acaaacagat tggcgaacat ggctatattc catttggaaa aatcaaggaa agttggaagc
    18721 tggttataca cgtgtgcaat gtagccgatt cgatattgcg ttagatgaac tttggggatt
    18781 ggaaacggaa gaacatttcg atctctttga attactagaa aaacaaaaag caggtttatt
    18841 agaaatggac tttaagagat tccagaatat tggcggttgt tttttagatg atggctattt
    18901 tcgaagtgaa ggattatctt tgtattttgg atcgcgaaag agtcctctct tttttaattt
    18961 ttatgagaaa cgttttgaaa ttgcgaaccg agaaaaaatt tcagaattag aagcattaac
    19021 gaaatatggt atttataatc gatatgagtt acggttagcc aatgagaaag caacacaagc
    19081 agtagaagca tttattttag gtgattcacc gaaacgttta ggagagattg gcgttggttt
    19141 aattaatgca aaacttattg tttgggatag actggaaagt gatgcacgga ttgttgataa
    19201 acgatgggca aaattatttc gttcgatgga agaattggat tttacagtaa agccagaaaa
    19261 attctcatta gatcgttcag aacgctggtt tttgaaacaa gtagctccga cattaaagtt
    19321 gttacaagaa gcagatcgta ttttaggaag agactttgta caagaaggga ttgataatgc
    19381 cgaactaaat gacgagcaac aaaactatct agaatggatg ggaacgtcag gagggaaaat
    19441 tgcatgaatg catatacgac aacagaatgg ctgcgcctgt tggatttaaa ggctgcaatc
    19501 gaagcattaa atgaaaagat ggttgatttg tcttattttc gttttcgagt tccttatatt
    19561 gaacaagcgg taaaagctgg acgttatcag gaaaaagaga actggcaaga aattgcacgc
    19621 ttattagaag ttcgtaaggg atacgaacaa gaactcgaag agttggaatt tagccgaagg
    19681 aaaggaaggc tagaattcat tcgcttttat cgattttcat tgccaatacc tgcgatttta
    19741 gcggttaaaa aaggctgcga taaaatgaag atatatgaaa actgtgtggc agcacttagc
    19801 agcgaaaaac ctcttatgga agaaatttca ttggtgactg tcacttggga aatgagtcgc
    19861 cagccaacag aagaacagac atatctttct ttagaagaga ttgagataga attagaggaa
    19921 attggtcgct atgccacttg ttctacctat tgtggatcgg tcattagtat tgctggtgtg
    19981 attgtctaaa tgaaaaaaat tatgatttat gttggtgcgt attggtcacg tgatccaaca
    20041 gtattagaaa atccagaggc ggtacactat tgcttgcgcc aattattcta tctgtataaa
    20101 gaacgattag aatcgttgat tcgtcagtta ccatatactg acagacgttt agatgaactt
    20161 cttctaaggt atccagcaat gtataaacga cgaaaaaata gattgttacc agaagagtat
    20221 cccattgaaa aacatgaact tgaagggcga tttgtggctt atttctatga tgatgttcgt
    20281 atgcgcttag tagaacaacg aatggaaata gaaaatgatc gctactattt tatcaattat
    20341 tgtaagaagc gtaagtatca agtaactgat gatttttatg attgtatttt acaagatggg
    20401 gaagtgattc tatccaaaat tgctccaagt tttcgtgaac ttgtgccgat tgattttttg
    20461 aaaaaatgtc atttgcggat tcttccgtaa aaaagagcta taaaaggaaa gaaaagtaga
    20521 agccaaatcg gtttctgctt ttttattttt tttaggaagg agaagatggt atgtcactgg
    20581 aatttttagg agagccaatt gaaaaggaag agttcgtttc gcattatatg gatttgttta
    20641 ttcaggtgat aaaaaacttt gtgcgattga tgaattttat ccacgcgaac aagactatct
    20701 agccgctgaa cgaataaaaa tcagtttatt atgggaaaaa ttcacagatg aaaataaccg
    20761 accgccagat tatcgctatt tatcagattg tttaacgaat aatgtgcttt ctaaagaata
    20821 cctatttcac aaacgtgaga aaaatcaaat gtcggttgaa catttttgtg attgttatat
    20881 tgcattattg cgtagtcaac agttattaac agtagaacga tatggtgcag agtttggtta
    20941 tgtcttgcaa gctgagcgtc atagagctac aagatgctat gaaaaagaag atcaagtgat
    21001 tgatggctat gaagaattat gtattcgaaa caatcgctat ttgcaacaac gtttatatga
    21061 acaattgatg atggattttt caaatctcta tagagggtat gaatgttcga tgattcaaga
    21121 aaggtagaag aaaaggggga atttttatgt ggcgattatt atttttggtg gtatgtggta
    21181 tggcattgtt ctatgtgtat cgtgtctatc tctatccacg aatggaacga tggacaagtt
    21241 ggtatagaga tcctttaaaa caacgaaaag gattgaagcg gtatcgttat gaattacttg
    21301 ttgaattttc ggatgggcga caagcacagt attatttttt tgcaaactat ccaaaataca
    21361 acaccagtga actattgatt caattattca aaaataatcc atatgttggg ttagaagaaa
    21421 acggacggga gttcttttat cgttcagata atattagtgc ggcgtctatg aacaaaacac
    21481 gaattgcata aaaaattgga ggaataatca tgaaaaaaac aacattagca gtcgtaacaa
    21541 cactaggaat ttcattattt tcgtttcaat tagggagttc attggtttat gcagatgaaa
    21601 ctgtagaaac acctaagtca gaaattgtag aaacattgcc aggggaagga ttaccagatg
    21661 ttgagacaga gctaaatgga ggaacaacgg aaaaaccagc agaacctgaa caattgcagt
    21721 cagagaaacc tgaaaaagag ccagtgacac cagaaccaga gtttcctgaa tcaactttac
    21781 cgactgattc gatcgaagaa gaaggtgtgc atagttcaac aacaacggat tcaagtatag
    21841 tcacaactcc ggaacaatca ggaacaacca gttcttctga aaagaatact gattcgtcaa
    21901 gtacggaaca gccaacgatg tcgtctacca gtgaagaaca aaaagaagaa ccgaaacaac
    21961 ctgttgaaaa atcagaagca attccaaaag aaaataaacc tattgtttca gcaaaagaag
    22021 tcacagtatc ggtaacccca agcggtgaaa ttacgacgaa tacttctcaa ggaatgagtg
    22081 taccgattgt cactagtaat gtagaggaat tgacccatat tccaacacca accaccccat
    22141 taaaagtagc aggtgatcaa acaattgttg gtgtaaaaga tggtattcca ttaatccaag
    22201 atagtgaagg gaatttggta aaggatttgt cgattcctgt aaagaaactt ccaagtggca
    22261 atattgaaat caaaacagca gatggaaaaa caaaagtctt accaaagact ggtgaagaaa
    22321 tacatgttgc actttctgtt ctaggaggag tgttgacaag tattgctgga tttgttggat
    22381 ataaaagaaa gagaaaagga atagaaaatg agggtaattg aatggactat ttttcagaaa
    22441 aacaagctta tcgttgtcaa tcgtacgtac tagtaaaaac atactagaag gagtagctta
    22501 tgaaaaaacg acaactacgg cggaaagaaa aagtttatac agagaaagta ccgaagcaaa
    22561 agaaaatcca ttcaaaaaaa gcagtgaaat ggatttggtg tttggtttta tttctttgtt
    22621 tttcaggggg cttggcattt tttcaatcca tgaaaacgaa ggttcaagtg tcgtttttga
    22681 aagatgaaat ccaacaatta caaaagaaag aagtacaaag tcggtcagaa ttaacgtaca
    22741 cacctgaaat tgaatccttt atgaatcgtt ttgttgcttt gtatatgaat acattcgata
    22801 atcatgaaca agaacaacaa cgtcaagaaa cattaaaaaa agagttttat gcacaaaact
    22861 ttattcaaga accaggaagt gtttcaggtg ttcgaaaatt aactgctgct tcgtttattg
    22921 gattaaaaga aattgaaacc attccaactg ctagttataa agtaacttat gatattcgct
    22981 cgatgaatcc agagagtaag aaagaaacaa gtgtgacaat cacacaagta ctcaatcttc
    23041 ccttttctat ttcaaacaag ggctgtcgta ttattgctta tccttatttt acggcactac
    23101 caaagaatac agaaaaacaa cgaatgatta cttatgatga cacattatac gaaacggttg
    23161 atgcgtcgga agcgaaagat gttgaaggat ttgtggaaga tttcttagat aagtatgcga
    23221 aaaacaatgc aaaagatatg tcatacatga tgaaagaacc tgaaggactc aatcaaacag
    23281 ctaaagttga tcaagtagag atgaaagtat tgcgttataa agaaacgaaa aaacctttta
    23341 ttgtaaaagc aacggtcgtc tttcgagatg gtgatacgtc acttttacaa aaagaacaaa
    23401 tgacgattgt cattacgaaa aaaacaaatc agtattatgt tgaaaaatta acacataatt
    23461 ggttggatag gaaaggagaa gaacggaaat gacactgaag ggaattgtag attttatatt
    23521 aacgcaaggt ggctattttt taataatggt ggttgtttat ctgggattgc gaaattttat
    23581 gaaatcaaaa gtaggaaatg cattaatatc tatcgcagca ggtgccgcag tttatttctt
    23641 cttaaatgat ccaacacgtg tatttgatgc gtttggtaaa atcattgaaa aattatttat
    23701 ggattaggag gatagtgaag tggattggga accttatgat tattcgattg tttatgcgga
    23761 accttatcgc gtacaacaga ttacaagcaa ttggcgtctt ccatttccag ttgcattagt
    23821 caatgcagca acttttttgt tggtcgtaat ctttgaatat ttatttttac gttcatttat
    23881 tggttgggtc atttcttttt ttccaccagc aggagttgtt ttttatatag gactccccta
    23941 tttatgtgtg ttagcactgg caaaactaaa acctgatgga aaaaaagtgc atttttattt
    24001 atgggattgg ttttgttata tgattcgtat gatgaatggt gattgtgtgt attataactg
    24061 ggacaaggta tcaaacaatg cattggaaga attacttttt gtgaataagt atgtttattt
    24121 agaggattag aaaaagggac aatcaactgg ttgttccttt tttattggga ggtgaaattc
    24181 attggtgctt gtaaaagaac cagtaaaagc attcaagaaa aattgtttgt taaatggaga
    24241 ggattgtatc tgggcctact atcatgtttt tccacgaaca atttcaaaaa acacaccaaa
    24301 aaagacacag gaaataaaag aaatgatggc tcgtttactg ttgcttttag cagattatga
    24361 agatgtggag ttggtgatgt atccacgaac aatgaattta cggacgaagt ttgacacatt
    24421 aggaaaggga ttttctcccg atagtcaagc aattggtgcg tattatgcaa aagaaacagt
    24481 gacattgttg gaacgagaag gtcaagtcta tcgtccttca tttattttag gaatcaaagt
    24541 caaagaaaaa gcaacggtgg agagttttca tgatgtaata aatctaatct ggttagattt
    24601 agcaggaagt ttagcacgct tatttggatt agaagtagat gatttggaaa ctttgttttc
    24661 tgaaagtgat ccttatgtaa aagaagtgac acaattactt tatagctttt ctattcgtcc
    24721 cttagtaaaa aaagagctgt tgtatctcaa tcgtctgcaa tatattcgag gaatggctca
    24781 caaagtagaa gaacaaaata attgggaagt actgaaagac aatcaaggaa cgtatgtacg
    24841 tataccgaag gaacataaag gccttcttca attagaagat gagttaggga gtagttattt
    24901 agcattgttg cctattgggg aaacacctga aaatatgaga aattgtgatc tatttcaatt
    24961 tgctcaggat ctttctttcc cagcagaatt gcgaatgaaa attcactatc cacgcttgaa
    25021 aggacaggga ggtattggtt taaaactgaa ttggctatcg gaacggttca aaacagagca
    25081 taaggaattg gttcaaaatg atgaagaacc tagtgaacgc ttattaactg ttcgaggatt
    25141 agtaaaacat ttaaagcaac gagtagataa tgggcaacct gtcgtcgatt ggatcgcatg
    25201 ttttattgta tacggtcaaa cggttgaaga agttcgtcaa aatagtgcaa cggttattca
    25261 acaacttcat acgattatcc atatccaccg tgcaaaaaat gatcaacgag aattgttcta
    25321 taaatgttta ttagggcaac cattgggacg gcagttacat tggaagcacc gaacaactgt
    25381 agaagcagtt ggcgaatggt tgttttcagc agtatcagaa ttaggtatgg aatccggctg
    25441 gtatattggt cggcaagata cattagggga tggtggttct cgaggagaaa aaacaccatt
    25501 agaagaatta atttatgcgt caaatcgttt tgtctttttg aatcttctag ccattgcgga
    25561 aggaatcaaa ggtgcgttgt ttgatgcgcc tcatgtagca gtaactggaa aaaccggtaa
    25621 agggaagacc tttttagttg gcttgctctt tttgtatagt tcgtttatga atgtacaaag
    25681 tttatttgtg gatcccaaag gagaaaagcg caaatggttt acgaaactca ttcaagaccc
    25741 gtattatcag aaacattacc ctttgtttgt agaacatttg caaaaaatga gttatgtcac
    25801 gttagatgct acaaatcccc gtaattatgg tgtattagat ccaattgttt gtttatcccg
    25861 agtagatgcg aaacaagtag cccaggatat gattgatgag ttaagtccgt taggtaccaa
    25921 tcacctttta aaaggggcgg tattaaaggg aatccaagaa gttttagttc aaaaagaagc
    25981 cggacaacaa gttggcttga tgcatgtggt cgactattta gaacaaatgg aacaaaaaga
    26041 ggttcgagaa tatggtgagt ttttacgctt gacggtagaa gattcgattt tacgtttagg
    26101 attttcttat ggagaaaacc cgggattaga ctttaatgcc aaaacaacga ttttagagat
    26161 ccaagatttg aaattaccaa atgacaatgt ccgaccagaa ctatatacag atgcggatcg
    26221 aaagtctttg tgtttgatga ttagtttagg tcgtttttgt gaaatgtttg gaaaacgaga
    26281 tagtagtaag aaaacagcga tttattttac agaagcatgg gtatttaata attcgaacgc
    26341 aggacgtagt attattgcag caatggcacg tgtggggcgt tcccagatga atcaattagt
    26401 gttagataca caatttattg gtgacttagg ttcagataaa gaaaaaggga attttggtgt
    26461 cgtctttgct tttgatgaag acagtgagcg agaaaagatt ttgaatcatt tacgtttgga
    26521 agtaaatgaa gcgaacttag ctgaaatgag ccacatgatt aaaggacagt gttggatgtt
    26581 agatccctat ggacgtgtag gaaaactcaa tgtacacagt ttgtttgaag aaatgaccgc
    26641 tgcattacaa acaacggaaa aaacaagtca ctctcaagta gaagaacagg atacgttaat
    26701 catgcaatag aaatgaggtg aaacaatggc ttatataaag cataaggaaa agaaatggtg
    26761 cgttttgttt tttttaacga ttggattcgg actgcttttt gttggtggat tggtgtatgc
    26821 ggcagaaaag caggtggatc aatcgatcta tgagattggg aaatttcata ttgatacctt
    26881 ttcttcttat ttatctgata gtgaattttc attaggcaaa atgttatttg gggcaaatgg
    26941 cacagatttt ctaaaaatga tgacgaatat gttctttagt ttaagtaaat tgatttggca
    27001 aggccttgat tttgtcattg agaagctcta tgaaggagct gcaatgaacg agttaattca
    27061 ccagtttttc actttttcgg atactattta ttcaaaaata tacgatccta ttggtgcggg
    27121 gattgtcgtc ttgtatgtgt tatatctatt tactcttcgt atatttagag ggaaacatta
    27181 cgcaaaaatg gtgtttattc gatttctctt cgttgtcatt ttttcaatgg tttggtttgg
    27241 ctatggtagt aacgtaccta gtaaaggaga agcttttacc aaagggttaa atgatttgtc
    27301 cgtggaggta gaaggcttta ttttttctgc tacaaatagt ctagatgaat tggcgacaaa
    27361 agatgtcaat ggtgttttag tgccaacgac acaaaaggaa gccattcaaa aagtgcgcca
    27421 actttattat aaagcggctg ttgttgatcc gtatatattg ttaaattatg ggacaacaaa
    27481 tttagaagaa ttgtatcaag cagagattga accacaagaa tttctagcca aggaagcgtc
    27541 aagtgaagaa ttatctgata ttgagacaaa agtgaccact gctgcaggag aagatagagg
    27601 gaaaaagaag gcttatcgtg tctatattca accaactaaa gctgtttata aagcagtcgt
    27661 tggttctatg gtgccattgc ttaatctaag tattggatta ccacttgtag tgattggcat
    27721 gattcgtttt atctttcaaa tgttggtgtt gtgtttatta cttgtgattc ctttttcatt
    27781 actcgcttct tttgttccag tatttgatta tatgctcatg aataccttaa agtccttttt
    27841 gggcttgatg tttcaaaaat ctttttacag tgtgattatt ttggtggcat ttttgatcta
    27901 taacatagta gacgatttaa ttccaatgaa tactgttttt ggttttttag gaaatatgtt
    27961 tgtcaaagca ctcctatcaa ttttactcat tgtaaaacgg aaagatttat taaagaaact
    28021 agggttagga gctgcagatc aaactataaa caacacgaaa aacatagcaa gtcattcttt
    28081 gtctcaaatg aaacaactgc ctggtcaact atcaactgct ggacaaaatt tggcaatcaa
    28141 aggtgcagga ttagcaggga atgtgtatcg agcaacgaat ccactcgctt ctgcagcgat
    28201 acatggattt caaaagtttc aacaaaaacg tacaccacaa gaatcggtaa aagggaggca
    28261 agtggttaat ccctttgaac taggtgggcg ttcaaatcaa gccttgcctt atagtggtcg
    28321 tcatgttcgt tatcatgggg tagagcctga aatgtcttct accaaatcga aaacagagtg
    28381 gaattctggt ctttcaaatg gtttgattcc aaattcgtcc atgaattcag taagaccaaa
    28441 acaacaggta caatcattgg cacaattgag taagcaacaa gggaataatc aaagaaaacc
    28501 attgttaaat aaaggtatcg cttttgttgg tcaatcgatt ccgttgcctt ctggaaaagt
    28561 acccgtgcca caattaacag ctgctggttc tctttcacaa cagattcgcc aagtgaaaaa
    28621 tcaggcgaaa aatgggagtt atccagcaat gcagcagtta ttgaaccaat actctgttcc
    28681 taattcgtcg gtagccactc tatcaggaaa tcgttctgtg caacaaccgg ggcaacgttc
    28741 gtctgaaagt aaaccgtcga tttccgcacc aaaatcgtta caacctgcat tttcttcttt
    28801 agataagcca acagagcaat cggaaaaaat agaaaaggat cctaaaacac tgccgatttt
    28861 ggacgcaaaa ccaggaactg attttacttt accaaaagga cgaacagtcc aaaaaccatt
    28921 atcgaagaaa ggatgaggaa gaaatgaaga aagggtgtct aggttgtctt cttttcttta
    28981 ttcttccctt ttttctattg ccattctttt tttcaggaga actttctgag catggaggag
    29041 aagctacagg tctagctgtc gcttttaatg gggaatatac cgaaggatta ccgatttttt
    29101 cagagattaa aggacgtggt ccaatcacag atgaacatgc gcaagcagca gttggtgcag
    29161 ctgtaaagta tcagttatta ccgagtgtga ttctttctca attaggctat gaatcagctt
    29221 atggacagtc acaatctgga agaaacgata cgaacttttt tgggattacc tggtttcctg
    29281 gttgtcccta cccacaaggt tctgcacgtg gaattggtgg ttctgaaggt ggtttctata
    29341 tgaagtttcc gaacttaaaa gcttgttatg actattatgg ctttatggtg gcgagccaaa
    29401 gcaactttaa tcaatgtgtg ggcaataaag atcctggaag ttgtttattg attctaggtc
    29461 gtggaggcta tgcggcagca ggtattaccg aagggagtcc gtattatcga ggagcaatga
    29521 gtatcataac gtcctataac ttaacggaat atgatacttt tgcgatcaag cattggaata
    29581 gcctaccaac aaaaaaaact tcgcttattg caggaaaaag taaaggcaat gcgaaggttt
    29641 tggatcggat cattggacga tccgtaaata atggggaatg ttatggtggt acggcatggt
    29701 atgtatctca attaggtggc ccacagttaa tgggttctgg acacatgttt gcgatgttaa
    29761 taggggaaga ttataactgg acaacctatg gttggcaagt aatcatgaac ccaaaaccaa
    29821 gtgatttaca agcaggagat gtggtgaatt ggcaagcagg tggtgcattg tctcctggaa
    29881 tctatggtca caccggaatt ataacagatg tttcatatgg cggacaacat ttctctagtg
    29941 tggaacagaa tgccgagcaa ggacggatta tggctcggta tcaacgcacg tacaatcaaa
    30001 caaaaattcg tagtctggtt cgtaaggtga aataagaaag gcagtgacta gaaacgtgaa
    30061 aaaagggata attttagtat ttagttggtt atgtttgata ggtcttactg gttgtcattg
    30121 gttttcttct gaaaaacaaa aaacacaatt gagaacgaca gaagaaacaa tcgaagcagt
    30181 aaaagaaaca agtggaactc ttcaaagtac tgaaagtaac agtcaaattg gttcattaga
    30241 agacgagaca gcgcaacaaa tgcaaataga tgaaatgaca aaaaatttct taaaagattt
    30301 tgctagaaaa tggagtaatt tcacagatgt ttataaacgc aatcaagctg tgagggacta
    30361 tatgacagat cgttgtatta aagacaatag tattgatgta gacccgcatg tacaaatgaa
    30421 aacaactggg aaactttacc aggtgacaca agatatagag aatccggatc aatatttgtt
    30481 atttggagaa gaaacaatga ataaggttac tcaatttgta ttacttcaat tgaaagtagg
    30541 tcaagaagct caaaagattg atgcgatcaa gatttattat gtacggagtg cgtattaaaa
    30601 taaaaaatga acactcatat ttattataca ccgaagaaat agaaaaaaac agactgtttt
    30661 tttaagaaaa actatgtcat tctttacttg aaaaaagttg ttataggaca gaaaggataa
    30721 aacaaaaacg gaaaaaatat gtcttatgaa taactataag aactattcta ttttttagga
    30781 gatggatacg agatgaaaaa actaattggc tatagtgtga tgtgtattgg atttgttggt
    30841 gtgggattgt ttgtatcgac tgaatctgct tctgcggctg aatggtataa tcctatctgg
    30901 tataatccga acattgaatg tcaaaaaggc tatccgtatg gaccgacaaa tctgaaaaat
    30961 ggaaaatgta gagtaaattg gaatggcgtt gtagatagtg taattactgg ttcaatggac
    31021 tctgctctac atggctttac gggtcactag aaggaggaag aaaatgtctg aagagaaaaa
    31081 agaaatcgtt gaatcattag tagctttaaa agattctctt tctaagattg aatatgttga
    31141 taggcaagaa attcaaaaac taattgatga tactattatt gaaatccaag atgcgcgttg
    31201 tgaaggtata aaaatttctg tagctttatc taaagtaata gaaaaaatga accgttcatt
    31261 agcgtttaat ggattgaagc tagatagaca aactagtctg atatgggatc atttaaaaga
    31321 cttgtatgac aagtctaaaa gaagtgaacg gactgctgta tcaatcttaa aaggcctttg
    31381 gggaatgaat tcataaacaa gatatttaaa aagtaactta ttttttatgg aggagaaatt
    31441 atgaaaagaa taattaaaat atttggtgtt ttaggtgttt tcttattagg tttaagtact
    31501 tttgttagta ctggaacaag tgtgtatgcc acagaaaaag tgaatacagt tgatttagaa
    31561 ataaagaata aattagtaga tgaattaact ttttattttg atacagttgg ttttttagac
    31621 gaacatggtg tataccatat aaaggatttt ggtgctattc gtgaacgaat cagtcaagat
    31681 aacgaacaag gaagagcagc aagagtcttt tatgaacata ttattgttcc tagaatggag
    31741 aaagttgatt ggaaacgata tgctggatgt gtagccatta attctcttcc tggaggagga
    31801 gtggcttggg ctataggtac gcagggagat tttattgaag cattaaaagc ttttaatttt
    31861 gaaaaagctg cggatttatt attaaaaatt gcagaaaaac aattaggaaa agctgcagct
    31921 aaagagttag ctaaggtaaa tattgtagca ggaattgcag cagctgctgc tagctgtgca
    31981 ttagagtaaa agtttatgaa aagtttagaa caagagcata tggcttttaa acaagcaatg
    32041 tttaaagaaa atatctattt aaaccataac tatattcgtg tttctaaggc atgttcacca
    32101 gtattgaata tgcttggagg tggaaatgga ttatatcatt tactgtttgt cgatgtttgt
    32161 tggttaattt ttttacc
//
LOCUS       DAYYGS010000027        31089 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000027 DAYYGS010000000
VERSION     DAYYGS010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.027"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..729)
                     /locus_tag="ACHA44_001144"
     CDS             complement(19..729)
                     /locus_tag="ACHA44_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HFM8904451.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(726..2198)
                     /locus_tag="ACHA44_001145"
     CDS             complement(726..2198)
                     /locus_tag="ACHA44_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8904452.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            2336..2809
                     /locus_tag="ACHA44_001146"
     CDS             2336..2809
                     /locus_tag="ACHA44_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8904453.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(2962..3513)
                     /locus_tag="ACHA44_001147"
     CDS             complement(2962..3513)
                     /locus_tag="ACHA44_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8904454.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            3618..4148
                     /locus_tag="ACHA44_001148"
     CDS             3618..4148
                     /locus_tag="ACHA44_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HFM8904455.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(4631..5074)
                     /locus_tag="ACHA44_001149"
     CDS             complement(4631..5074)
                     /locus_tag="ACHA44_001149"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904456.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(5090..6250)
                     /locus_tag="ACHA44_001150"
     CDS             complement(5090..6250)
                     /locus_tag="ACHA44_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8904457.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            6757..8457
                     /locus_tag="ACHA44_001151"
     CDS             6757..8457
                     /locus_tag="ACHA44_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904458.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(8559..9392)
                     /locus_tag="ACHA44_001152"
     CDS             complement(8559..9392)
                     /locus_tag="ACHA44_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFM8904459.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(9696..11861)
                     /locus_tag="ACHA44_001153"
     CDS             complement(9696..11861)
                     /locus_tag="ACHA44_001153"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HFM8904460.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            12121..14415
                     /locus_tag="ACHA44_001154"
     CDS             12121..14415
                     /locus_tag="ACHA44_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFM8904461.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA44_001155"
     CDS             14408..15073
                     /gene="pgmB"
                     /locus_tag="ACHA44_001155"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8904462.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            15066..16073
                     /locus_tag="ACHA44_001156"
     CDS             15066..16073
                     /locus_tag="ACHA44_001156"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8904463.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            16095..17114
                     /locus_tag="ACHA44_001157"
     CDS             16095..17114
                     /locus_tag="ACHA44_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8904464.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA44_001158"
     CDS             complement(17395..18762)
                     /gene="nhaC"
                     /locus_tag="ACHA44_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFM8904465.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHA44_001159"
     CDS             complement(18871..20292)
                     /gene="tyrP"
                     /locus_tag="ACHA44_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070908 - tyrosine:tyramine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-tyramine antiporter"
                     /protein_id="HFM8904466.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHA44_001160"
     CDS             complement(20535..22412)
                     /gene="tdc"
                     /locus_tag="ACHA44_001160"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8904467.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHA44_001161"
     CDS             complement(22703..23959)
                     /gene="tyrS"
                     /locus_tag="ACHA44_001161"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFM8904468.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(24071..24276)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            24539..25840
                     /locus_tag="ACHA44_001162"
     CDS             24539..25840
                     /locus_tag="ACHA44_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8904469.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(25909..26508)
                     /locus_tag="ACHA44_001163"
     CDS             complement(25909..26508)
                     /locus_tag="ACHA44_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904470.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            26762..27643
                     /locus_tag="ACHA44_001164"
     CDS             26762..27643
                     /locus_tag="ACHA44_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8904471.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            27656..29266
                     /locus_tag="ACHA44_001165"
     CDS             27656..29266
                     /locus_tag="ACHA44_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904472.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(29343..30002)
                     /locus_tag="ACHA44_001166"
     CDS             complement(29343..30002)
                     /locus_tag="ACHA44_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8904473.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVALYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(30285..30950)
                     /locus_tag="ACHA44_001167"
     CDS             complement(30285..30950)
                     /locus_tag="ACHA44_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HFM8904474.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         9296 a         6341 c         5644 g         9808 t
ORIGIN      
        1 tacatggtgt tcaaaatatt attcgccttt tgcttctttc actaattttg cgaatgcttg
       61 ttgttggaat tcagcagcta aggctttcgt acgttgttct tgctctgctt tgctcatttt
      121 tggattttca gtcattgctt ttaccatacc gtcttgaaca taagcttttg cttcactttc
      181 catcgtactt tgctgtttct tcactaattc agctaacttg gctgcttggt ccgtacttaa
      241 ttctacccat gtctctggga ttttgaatgt attcactcgg ctgtcatatt cgtgattgtt
      301 atcagcaata ttgaaccaga atttatacat gtcgtttttg tattcccaat aagtagtttt
      361 cgtcactttt tcagcatcgc cgtctgtctt ttctacttta tttgtttcgt tatccgtacc
      421 agttgctttt ggtttttctt gtttttcatt tgttcggtaa agatacactt tttcttttcc
      481 attccctaaa gctttataca aaagcatatc tgctccttgg gaatcagcac tggaaacaat
      541 tttttctgtt tttgtttccg ttactttttc cattccaaag tgattggaca aattagcgac
      601 gatcaaactt agtgaagcga cgaagattag tccgaaaatc aaagataaaa aggtttgcca
      661 tgttttttta gcgaacacgt tcgttaaagc aaaagcaagc aagctgacaa tcaaaactcc
      721 aataatcatg cattatcccc cgctttcatt agaacaggtt cttttttctt caataaaaag
      781 gcaataataa agccaagaat cccaaagatg atcgctacga taaacgcggc atggtaacct
      841 gacagtgtgg cattgattgc ctgatcttta taagctaatg gcgtattatc caatacagat
      901 ttgcttggga tatttgcttt tgtcacattc gtcaacacac tgatcaaaat agctgtacca
      961 atcgagcttg cgatttgacg gaatgtatta tttaccgctg tcccatgact gatcaagtta
     1021 tttggcaatg cattcatacc agaagtcgtt accggcatca tgaccatgga gatcccaaac
     1081 atacggatcg cataaaggat cacgatatat aaaactggag tcgtttttgt caagaaagca
     1141 aatggtgcag ttgccgctgt caaaatgaac atccctgtga tggctaaacg tttcgcgccg
     1201 tatttatcga aaatcgcacc cgtcacaggc atcatagccc ccatgatcaa tgcacccggc
     1261 aacaacatca agccagaatg gaatgctgat tctccacgaa tattttgaat atacaatggt
     1321 acgaccattt ctgcccctac catcgccata tttgttacac cagctaaaac agctgcaatc
     1381 gtgaatgtag atgatttgaa tacacgaagt tccaagaatg gatgttccaa atgtaattga
     1441 cgccatgaga acaaggcgat gaaaatcaca ccaacaacta agaaaccaat tactttcaag
     1501 cttccccagc cgtcatctcc tacactagaa aatccgtaaa gtaaactacc gaatccaatc
     1561 gttgataaaa tagcggataa aatatcaatg ctaggatttg acaatggaat aacacttttc
     1621 attaggaaga atgctaaaac caagacaaga acaacgattg gcagtaccat accaaacaag
     1681 tcacgccaat ggtagttatc aatgatataa ccagataatg tagggcctaa agcaggagca
     1741 agtccaataa cgatccctga catccccatt gctgatcctc ttttttctgg cgggaaaata
     1801 gatagcatga tattttgcaa taatggcatc gatactccca caccgcaagc ttggatcaaa
     1861 cgaccgatta gtaatgtact aaaattaggt gctgtaaagc aagtaatcgt tccgatcaaa
     1921 aagatcgtca tggcagaaag atataaattt cttgaactga atttattgat cagccatgct
     1981 gttattggga tcatgatccc gttgactagc aagaaaccag tcgttaacca ttgcacatca
     2041 gacgctgaaa tatcgaaagc tttcatcaat gtaggaaaag ctgtcgtcaa taaggtctga
     2101 ttcaaaacgg tacaaaatgt accaattaat aagacaacaa ctaaaagtgt ccgattatat
     2161 ggttttccgt aaatatctac gggttggttg ttactcatat ttctccttct ttctgaattt
     2221 tttctgaaac tactagaata ctttagtcct aaattcgcta tttgtcaacc tacttgacac
     2281 ataagttaat acggatataa ttaagggaag caagaggaca aaaggagtga caaaaatggt
     2341 tggggcttcc cttaaacgtt tgttggaaaa tgatcaattg gttgttggca tcagtgaatt
     2401 aagtgatatc gtaggtgtgt ctccacgtca attaaggtat tgggaacaaa agggattcat
     2461 tcgttcgatc gaagctgatg cgaattgtcc tagaaaatat cgtttaccta cagtcataaa
     2521 agtagaactg atcaaaaaat atttagatga cggatttact ttgtcaaaag ctgcagaaaa
     2581 ggcagaaatc cgccaaaaga aaatgcacca tgtccgtaaa gttttttcaa aagcaatcaa
     2641 agatatcgaa ttgatcgacg aacgctatac gattttacaa atcggtaact tcaaagaaga
     2701 gaatgctaag atgtacatca ttcatgacaa cgagacagac gaattaactt acaagctcct
     2761 tcctgtcgat gaaaaagctg actacgaaca gttgttaaaa gaactctaaa cgccactaat
     2821 ctcctaagta ccaagctttt taataagtag atgctacata aatacttgaa aaaaggcctg
     2881 tttgaacata agaatatccg aacggtgatc tgcgtttctt tcatatagaa aggaattctc
     2941 catcactgtt cggatatttt tttatttttt tcggttactt tcttctaata catctgttag
     3001 ccaatttgca tattctgttt cccgattgat cgcatgcgct aaaatcaggt agtgtccgta
     3061 attttcatgt ttttcttttt cagtaggaaa gatcgttttt tttcgctcta atagatgaag
     3121 tcgtttttct tcatgaaggt cacgctgctg ctgaataatt tctactaatg ccggatcatt
     3181 cacgtctttg acgaaataca atttcagaac aaattcgtct cgattaaccg gtagttcatt
     3241 ggtgggtgta tgaatccact ctgccaattg ttccttccct tcttctgtta attcataaac
     3301 tttcttttcc aatttttcac ctgtgatctc tacttgatgc tgaatgattc cttgctcttc
     3361 catttttgca agttcaggat aaatctgact atgttttgct tgccaaaatt ctcctatttc
     3421 atttttgaac gattgattca attcatatcc tgtcatcttc ttcttactta ataaccctaa
     3481 caaaatatat cgaagtgtat tttttcttgc catcagtttt ccctccttga caaatcttta
     3541 aatccattct atcataaatg agtaatcgaa aacatgtagt ttattacatg ttttaattta
     3601 taaggagatg cttaaacatg aaagaattta aagaattgga agattttatt ggaactcact
     3661 ttatctatac gtatgacaat ggttgggaat atgaatggta tgccaaaaat gaccatactg
     3721 ttgattaccg tatccatggc ggaatggtca aaggccgttg ggtaaaagac caagaagctg
     3781 atatcgttaa attgacggac ggcgtattca aaatcacttg gacagagcca acagggacag
     3841 acgtggcatt ggactttatg ccaaatgaaa agaaacttca cggaatgatc tttttcccta
     3901 aatgggttga agaacatcca gaaatcactg ttactttcca aaacgaacat atcgcagaaa
     3961 tggaagcagc tcgtgaaaaa tatgaaactt atccaaaatt acttgttcct gaatttgcta
     4021 cgatcactta tatgggtgat gcaggtatag acaatgaaga tgttattagc gaagaaccat
     4081 acgatggaat gccagatgat atccgtagcg gaaaatactt cgatgaaaat tataaacgag
     4141 taaaataagg ttgtgaaaga gaggttgtga aatcagcgtt ctgacctgag tattagagaa
     4201 agaaacggaa aaatagttcc tcatgttttg ccgttttttg agctaatacc gagggtcagc
     4261 tgattgaaca ccgtttatca agaggttgtg aaatcagcgt tatgctttga gtaataagaa
     4321 cgctagttag aaaatagctt ttcatatttt ctaggctagt gggcttatta ccgagaagca
     4381 gccgtttgaa caccgtttat caagaggttg tgaaatcagc gttttgacct gagtattaga
     4441 gaaacaaatg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
     4501 agctgattga acaccgttta tcaaagattg taacaactgc atattcacac agttaaatta
     4561 gtgacttcaa aatgaaataa tgctcccagc aaaaaattag gctgggagca ttatttcgtt
     4621 attctgttca tcaaaaatca gcgttttgat tccatttcat cacaagttcc tcctcattta
     4681 ctgcttcatc tttttgctgt gtttgaattg taaaaccttg tgaaagatag aaatgaatag
     4741 ctgattgatt tttttgatag actgtcaaag tcagtgtttt attggtttct tttatttttt
     4801 tcattagttg cgtaccgatt ctttgttgcc ggtattccct tttcacaaag attcctgcga
     4861 taaactgatc aatcacccca gcaaaaccaa taatttcttg atctatttct gctaagtaaa
     4921 tcactgcatc tggtaaagct ttcctaacag attctacttg attctcccaa taagttgatg
     4981 gaatgaattc atgagaatcc aagttagcat gcagccaaat cgatagaaca gattccagtt
     5041 ctttggcaga caattttgtt gttgtgcgga tcattttttc tctccttact tattcattga
     5101 ttttaaaata aaaaccattt ttttgaagct cttttacaag ttttcgcccc tcaaaaaagt
     5161 atatcttctc ttgttttact tttctaatac caaaagaaac agcaacttta ggatcatcag
     5221 ctgaaacgat tcgataagtt ccaccaggca attgatccag cttgactttc ccatgatggt
     5281 cgctttgtac tgaaaacggc acaccatttc tagtaagtgt ttttttctta tgtttcaaaa
     5341 tgaatgaact accatttacc ggtttccctg acgaatcact aataataaat gaaaaatcaa
     5401 gcactttttt acttaatcta tactgataga ttagtcgtcc gataaacact aacagtatga
     5461 ctacagctat tatcgttcct gcaagaataa accattgccg catggttctt tcctgatttc
     5521 cttttccttc ttcttttttt accgattcgt tataaggaat ccgatgtcct gtcactagca
     5581 aacgatgcgt gttgatcata tacggcgtac atgtaagcaa ggtaactaag tcttctcctt
     5641 ctgaaatcgt tagaacagac gtgtcatctg gcagtaccac ttgtatattt ttaacttgat
     5701 aagccaattt ctcccccaaa acagtcaaaa taaacgtgtc tccgttttct aaacgatcta
     5761 attcggtaaa taaccgtcga ttaggtaaac cgctgtgagc agagatcact gaatgcgtcg
     5821 aatttccacc aagcgggaaa gaggttcctt gcaagacgcc tgctccattt tcaaggatcc
     5881 taccattgag ccgatcataa agtgaaatat ttaattggat cttaggtata ttgatacttc
     5941 cgatcagcca ttcctctaat tggctggatg ttgtactttc atttctgttc ttttcattga
     6001 aaggatcagt accaggattg aatccttggt cagttaatcg ttggtttttc tcctccattt
     6061 tctttcgctg cacttcaatt tcagctgcgg tccgtttttg tacttgttcg atacgttttt
     6121 gatcaataaa actatttaaa gaatccacat aaaatggata taaagcgatc agtaacccta
     6181 cgacgataca tattaataaa aacaaacgaa acaaaagtcg acgagactgt ttttgcttac
     6241 tctgcttcac atgtgtcctc ccttgcaagt ttttttgttc tttcttttat tatatgggtt
     6301 caggaaaaaa atagaaaatg aaagccgatg aggttgtgaa aaagctgtcc tgcatcaagt
     6361 aataagtaca gcccaaaaaa atagctactc atattttttg ttgggttggg cttattaccg
     6421 cagatgcaag cttttgaaca ccgtttaagg aagaggttgt gaaaactgcg ttatgctttg
     6481 agtaataaga gcgctagtca gaaaatagct tctcatattt tctaggctag tgagcttatt
     6541 accgagaagc agctgattga acaccgttta ccgagaggtc atgaaaaagc tactttgttt
     6601 cgagtattaa gaaaaattct gtttcaaaac aaggagatgc aacaaaactt atgtcgcatc
     6661 ctctctcctt agaactttca cttatgtttt tcttgtaaaa ttcataaata ttttcatgaa
     6721 ttttctaatt tcttgcctac ctctcgcata ggatatatga tgaatttatc taaagtttgc
     6781 aaaggagttc gacaattgaa aaaatatggc ccatttttag caattttttt cattgctttg
     6841 gcatttctca tgcctcattt attcacacat aaactaattt tgggtgctga tgccccattc
     6901 cattacaatc ggttctatga tgccgcgcaa caaattaaat atggggattt ccattatttt
     6961 ccttcccttt atggattcca gcaaagtggc aggatcgtga atgccgttta cggcccattt
     7021 ttcgcttatt tacaaggatt gatcgtgttg cttgcaggta gctggtttca ttatcagatt
     7081 ctttcaaatt tcattcttta tcttatttca ggattttcta tgctgatttt actgagatat
     7141 gtcaatgttc gaaattgggt aagtgtacca gtagccgttt tattcatgag tacgtattct
     7201 attcaatact ggtctatcaa ccaaggattc actagctggg ggacttgttt ttatccttta
     7261 tgtatgatcc ctattgctga tttcgtcctt cataaaaagt tccctgttat caaagtcgct
     7321 gtctcagtgg gacttatgac acagatccat ttactgtctt cgatcatgtt agctttgatg
     7381 tatatccctt tttatcttta ttactttttt aaccagactg aaaaaggaaa agcactgctt
     7441 caactgatta agtcggtctt gctttatttt gttctgacag caaatatttg ggcaggatta
     7501 gctattgttt acagtgggaa taagattctt cctccctttg ttaacagaga aatgagtcaa
     7561 aaggcgatag atctggatgg cagttattgg ctccattacc ctaaggcctt tcctatattg
     7621 atattatcag gttttttgct ttttattttc tttcaacaaa agaacacctt attccaaaaa
     7681 atcgtgttgt acacaagtct tgttttcttt tttctttcta ctagcttgat tccatggacg
     7741 acattgatcg aaaaaaatgt gccttttatt agtttgatcc agtttccatt tcgatttttc
     7801 gtgccattta cgctattgct tctactttac cttgcccttt cactgaacca gtggtctgaa
     7861 caaaagtggt tccgtctttt aagtatattt ggcattctta ttttttcagc acagacgatc
     7921 cagaacttat accagcatct ggaaaagtgg gaaaatgaaa catttgtcag tcgacatact
     7981 tatctattcg atacgccgga agaagcaagg aaaacattct tcagcaaaga tatgtctacc
     8041 agcttgttcg ttttccaaaa aaccacacct gactatctgc caatctataa ggagacaaaa
     8101 gacaataaat acgaccggta taacgaagaa atactagaaa acgaaacaac ttttataaaa
     8161 acacatccag gcggtctttt gacaatcaag tggaaaaaca ctggggacaa aactgttcat
     8221 atccctgtta tcgtctacga tcggactgtg ctacagcaaa atggaaaaac attgactgat
     8281 tatgaagtaa cagatatcgg gaccccaatc gtgaagcagc aaaaaggaat caacgagctc
     8341 acgcttcatt atcaaacgcc aatctatttc tatttcattc tttctcttac tttgattggc
     8401 tggtttacct tgctatgttt atttatttat catagataca agcttttacg tgcttaggca
     8461 aaagggataa atacatccga tcaaaaaact ctccaaacgg tataatcgtt tggagagttt
     8521 tattttaatc agttttttta tgttatatgt tatatttttt attcgttcac atctaattcg
     8581 atttccaccc cgtagtgatc actgatgatt ggactatcga tcccatcaaa aatccgttga
     8641 tattttttaa cctgcattcc ttcagggacg aaaataaaat ctatacgcaa tttttctgta
     8701 ttttgttccc aaccatctat tttcttttcc acagtagctg cttttccttt ttcattcgcg
     8761 acaacatacg aatcttgaag aagaaggcaa ctttctctta cagtataata accttgagag
     8821 tctactggat tattgaaatc acctaggaaa aaaagtggta cgcgagtttc caaaaagtag
     8881 ttttccaagt ttttccactc aaaataaaag ccactttttt tatctgtcca ccaagaaaag
     8941 tggcagctcg agacaaaaat gtcctgatca tctatctttg ttttcccaat caagatcttg
     9001 cgtgtctgat aatcttcttt cttttgaaat tgggagacgg taaggacctg actttccaat
     9061 gtttttttcg taagcagtgc atttccttct tcatatttat cataaccgat gtggctcatt
     9121 tcccaactcc aaaaatactc ttttccgtgt tcctttaaaa gctgaactaa acggtaggca
     9181 aaattatctt tacggatagg tacttgattc tttaccggac agaacttcat tagcttcgca
     9241 ggttctactt cttcagattc cagtaattgg ttgatttctt gtaaagcaat cacttcataa
     9301 tcttctaaca ggatcttatc tgctaattct tgcaattttt tctctggttc tggctcaagc
     9361 cagctatgcg tgttgagtgt catgaatttc attggttttc tccttgtctt catttcgaaa
     9421 caaaagggtt gtgacaaaat ttttgtcaca acctctccag caaatagtgt tctcctgcta
     9481 actcctcagt aataagctca atggaccagg agctattttt tgtttggctg tttttatcac
     9541 ttggtgcagg gcagcttttt cacgacctct ccactaaaca gtattcaaac tgctgcttct
     9601 cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat
     9661 gacttgatgc aggacagctt tttcacaacc tctccttaca ttttaatgga gccgatcact
     9721 ttaccagctg tcactgtacc taattgcttg atcactactt cttctacttt atcgtttgta
     9781 aatacgatca tgatcgttgg atctttttct tcgttttgga tcacatcaag atccatctca
     9841 gctaatttcg ttcctgttcc tacttcttgg ccttctttga cagcaatctc aaaagctggc
     9901 tgattcattt ggactgtatc gattcccata tggatcaaaa cttcgatacc atctttcgtt
     9961 tccagcccaa ttgcatgttt gctagggaaa atacttgtca cttttccagc gatcggcgca
    10021 acaacttctc ttgatgttgg aataacagca aagccgtcac ccatcatttt ttgtgagaat
    10081 accggatctt ttacttcaga taaaggaatc acttctccag aagctgcgga aactagttct
    10141 tgttctatac ctaagtattg cttactacct gcttgtccat ttcctgtaga aaccatttct
    10201 ggctctggaa tctctgctcc tgattgcagc agatcttcga tatctgattt caagacatct
    10261 gcttttggtc cataaactgc ttggactcca ttgtctttga cgatcaatcc catcgcccct
    10321 gctcgtttcc aggcttcttc agtccctact ttttctcgat catttacact tacgcgtaac
    10381 cgagtcatac aagcatcgac atctttgata ttttcttttc ctcccagcaa atgaatgatc
    10441 tggaccactt gctgatctgc tgttcctgtt ccgctgttag atacaacggt ttcttcactg
    10501 tcattgtcgt aatttccgtt acgtcccggt gtagcaaagt tgaatttttt gatcaagaaa
    10561 tttgctaaga aatacgtaac aacaccaaaa gcgatcacac acaacacgaa gttgataaga
    10621 tctccaccta gacccgcttt gatagctaac ggtgtccgag tcagcaattc gatattccca
    10681 aatgaatgga cccgcaatgg caagatatct gccatcgcaa atgcagcccc ttggatcact
    10741 gaataaatga catataatgg cacagcagca aacataaaca tgaactccaa tggttctgtt
    10801 acacctgtta gaaagacagc caacgctgct gaaaaataca ttgatttata tcttgctttc
    10861 ttatcaggat cgacattacg atacatcgca aaagccattc ccatcaagat acctgaagaa
    10921 ccaatcattt gtcctacttt gaaacgagcc ggcgtccagt tatgcaacac aaactcatat
    10981 tgcgacatat cccctgcacc tttaagattc actaaatcag tggcccaagc caaccataac
    11041 ggatcttgcc caaatacttg tgtccccgcc tgtgcgcctg ataaaatctc atacgatccg
    11101 ccaagctgtg tatagttgat aggaatcgtc agcatatgat gcaatccaaa tggcaatagt
    11161 aaacgttcta atgttccaaa taaaaacggt gctagaatcg gtgcgctttc ttgtgattct
    11221 gcaatccata aaccaaaatt attgattcca gcctgtacgt ttggccacac cacagcaaga
    11281 atcaatgcca cgattgttga ccataagata acaacaaacg gaacaaagcg ctttccattg
    11341 aaaaatgaca aagcatccgg caattttcta taattatagt acttattata agccattgcc
    11401 ccgacaaatc cagcgatgat ccctacgaat actcccatat tcaaagctgg tgcttccaat
    11461 acacttgtaa aaaatccttt aaccatgatc ttcgtaccga acagagtgtg ggtaaatgca
    11521 tcttcattcg caagcatgtc acttgtgaca ccaaagatag ccccagtgat ccgattgatt
    11581 aaaataaatg agatcccagc tgcaaatgct ccgcctgctc tttcttttgc ccaacttcct
    11641 cctattgcta aagcaaataa aatatgaaga ttaccaataa ttgcccaacc aatattttca
    11701 attactccgc ccgttgtgac aagcggcgtc caatcaggat tgatcaaggg aattgatttc
    11761 cctatactga tcatcaaccc agctgccggc ataactgcta ccacaaccat taaagccttt
    11821 ccaaattttt gccaaaactc aaaactaaat atctttttca ttccgtttag ctcctttacg
    11881 caatcggttt cgttaaacgt attatagcgt gcgttttctt ttttgtaaat gctttatttt
    11941 ttatttttct gtctatacca ttctataaaa cctataatac gtgtgttccc ttctataatt
    12001 ttctcctaaa aatttatttt tttaagcttt tttctatgaa atattttctt ttgtctattg
    12061 acttgtgcaa tcggttgcgt ttattatgaa cgtataaaac agctcattag gaggagaaaa
    12121 atgaaacaga tcaaacgact atttgctatt gatccatgga aaatcagcac gacacaatta
    12181 gataaaaaaa atcttcgact gcaagaatct ttaacaagta ttggaaacgg ttatatgggg
    12241 atgcgcggga actttgaaga aacttactca ggggaccacc atcaaggtac ttatttggcg
    12301 ggtgtctggt atcccgataa gacacgtgtc ggctggtgga aaaatggtta tccagaatac
    12361 tttggaaaag tgatcaacgc cttgaacttt atcgcgatag atatttcgat caacgatcag
    12421 ctgatcgact tggcttcttt ggaaccagaa gacttttact gggaattaga tatgaaaaat
    12481 ggtgtattat ctcgtacatt cacaatcact acttctacta ataaagtacg catttccttc
    12541 gaacgttttc tgagcattgt caaaaaagaa tcagcttata tctatttaac tatagaaatg
    12601 cttgaaggta gcggaacagt caaactcgta tcaaaattgg atggaaatgt tcaaaacgaa
    12661 gacagcaact atgatgaaca tttttggaat gagattgatc gtggaactat agaagaaatc
    12721 ggctatttga cgactaaaac tattccaaac aactttggga ttgaacaatt tactgttact
    12781 gcagcaatgc gccatttcgt taatggaaaa tcagtcatcc ctgactatca cgaagatact
    12841 ttagcattac atggttgctt gtctgcagaa ttatctcaag gtgagacatg gtcagtggaa
    12901 aaagaaatta tcgttcttac tagtagagat attccagaag cgcagcaagc agaaaaagct
    12961 gtctcagaat taacgaacca gacaaacaat tatatccaag caaaagaaga gcaaacagcg
    13021 gcttgggcag aacgatggaa gcttgccgat gtggtcatcg aaggagacga agaagcacaa
    13081 caaggtattc gattcaattt gttccaacta ttctctactt actacggcga agatgaacga
    13141 ttgaatatcg gccccaaagg attcacagga gaaaaatacg gcggagccac ttactgggat
    13201 acagaagcgt atgctgtccc tctttatctt gctttagcca aacctgaagt aactaagaat
    13261 ctgctgaaat atcggcataa tcaactgccg caagcaatcc acaacgctca gcaacaagga
    13321 ttaaaaggcg cgctttatcc tatggtcacc ttcactggtg tcgaatgcca taacgagtgg
    13381 gaaatcacat tcgaggagat tcaccgaaat ggcgcgattg cctatgcaat ctacaattat
    13441 acgaattaca ccggcgatac ggaatatttg aaaaatgaag gactggaagt tttagcagaa
    13501 atcgcacgct tctgggcaga ccgtgtccat ttttcaaaac gagcaaataa atacatgatc
    13561 cacggtgtga ccggcccaaa cgaatatgag aacaatatca ataacaattg gtatacaaat
    13621 acgatcgcag cttgggtctt gcgttacacg cgagaaaatt atttaactta tcaaaaagaa
    13681 gctgctatca ctatcagcga agaagaacta gccaaatgga aagatatcgc agagaacatg
    13741 tacttcccag ttgacgaaga attaggaatc tttgtccagc atgacacatt cttagataaa
    13801 gatctcatgc ctgtatccga tattcccaca agcgatcttc ctctgaatca gcattggtct
    13861 tgggacaaaa tccttagatc ttgcttcatc aagcaggcag atgtactaca ggggatttat
    13921 ttcttcaatg atacttttac aaaagaagaa aagcaaaaaa actttgattt ctatgaacca
    13981 atgactgtcc atgagtcttc cctttcgcca agtatccatt cgattttagc agcagaatta
    14041 ggaatggaaa gcaaagctgt agaaatgtac cagcgaacag ctcgcttgga tttggataat
    14101 tacaataacg acacagaaga cggcttgcac atcacttcca tgactggtag ctggttgtcg
    14161 attgtccaag gatttgctca aatgaaaact gcccatgatc agttgagatt cgcaccattt
    14221 ctgccaaaaa cttggaaagc ttattctttc catatcaatt atcgcggacg tttattgttc
    14281 atctcagtca cagatgaaca agttactttc gagttattag aaggttctcc tctagaaatt
    14341 tacgtctatg aacagtctta ttctttagcg gatcgtctag aaataacgct aagaaaggag
    14401 gaagcaaatg tttaaagggg tattgtttga tttagacggt gtcattaccg atactgctga
    14461 attccattat cgtgcttggc gaaagcttgg tcaggaaatc ggcattacga tcgaccacac
    14521 attcaacgaa cagctaaaag gcgtcagccg agaagactca ctctctttgc ttttagctta
    14581 tggcggaaaa gaacactctt tttcaaagga agaatttgca gaattagcga aaagaaaaaa
    14641 cgattattat ttagagatga tccagacgat cgaaccaaaa gatgtcttcc ccggggtcgt
    14701 gcctttactg gattctttaa aagaagaaaa aatcaagatt gctcttgctt ctgcaagtaa
    14761 aaacgggcca ttcctacttg aaaaaatggg tcttacacct tactttgatg ctattgccga
    14821 cccggcagaa gcaacaaacg gaaaacctgc gccagatatc ttcctccttg cagcaaaagc
    14881 tgtcggacta aaagcagaag aatgtatcgg gatcgaagac gcgcaagccg gtatccaagc
    14941 catcctctca agtggcgctc aaccaattgg cgtgggacgt gctgaagacc ttggagagga
    15001 gatcccgctt gtccctgata cgacttttct tacgatcgat tacttgaaag aaaagtggca
    15061 tgaccatgga taatccagag atctccgtcg taccagttcc agagactgaa ataaagaaaa
    15121 tcacattgaa gaacgactac ttagaagtcg ttcttctcaa ttacggtgcc cgcctgcacc
    15181 aaatcttcgc accagacaaa gaaggaaaat cagaaaatgt tttattgtct tacgatcgtt
    15241 tcgaagatgt tctaacagac aaaagctttt ttggagcaac tgtcggacct gtagccggaa
    15301 ggatccggga tgcttcttgg ggtgagcata gattggagaa aaactgcgga tcacaccata
    15361 tccacggtgg cacgaaaggc tggtctttcc aattttggga agtagaagtt ttcaaaactc
    15421 catcatcaat aggtgtggtc ttttacttaa aagatgaatt ttctacctat ccaggaccaa
    15481 tcactgctac gatcacttac cgtttaacaa aaaacgaatt agagatgaca acctctgcta
    15541 gtagccttgt tgaaaccatc tgcaatccga ccaaccatgc ctacttcaac ctttcaggaa
    15601 atggaacaag agacatctat gaacatcagt taacggtctt tttagatggt gtgcttgaat
    15661 tagatcaaga aaaactgcct actggaaatt ggacaaaaaa agaaaaactg ccaatcgact
    15721 ttagaaaatc cccaacactg caagagatcc ttgcatgtta tccagatgga ctagatgatg
    15781 tatttctttt gcatcatcct aggctttcca aaacttcctt acaattattc gagaagcatt
    15841 ctggtcgcca aatgactata gcaacttcta ataaaagtat ggtattattt tctacgactg
    15901 gattcgaagc tgatttctct gtcaatggaa aacagatgca ttcaaactac ggattagcaa
    15961 tcgaaccgca agaaatccct gatatcgttc atttccccaa atttggatcg atcaacctcc
    16021 atcctggtca agaacggatc agtcagacga tttatcggtt ctctgcacaa taacaagaga
    16081 aaggtgtttt tatcatggct attaccgtaa aagatgtggc gaaaaaagcc ggtgttgcca
    16141 cctcgaccgt ctctcgtgtc atcaacgatc atcctagtat ttccgagaca acaaagaaga
    16201 aagtccgaaa aattatggat gaactaggtt atgttcctaa catgactgcc cgcaatctag
    16261 gaaaacgcat ttccagtgcc atcggcgtaa tcttaccccc tcttgattca aaagagcgga
    16321 tcggtaaccc attttatctt gagatcatgg aagcaatcaa cgaagaagcg cggaattatg
    16381 atatgactac agcaatcgct actgctaaaa attttgaatt gctgttagag aacgtccagc
    16441 gtatgcattt acaaaaacaa gtagatggtt ttattctagt gtattcagat agtcaagata
    16501 ctgtcattga ttatttatac caaaaccaca tccctttcac tttgatcgga cagccttatc
    16561 atcatgagaa cgaaatcgtt tatgtagata atgataacca gcttctaggt aaacaagcaa
    16621 cagaattttt gatccagaat ggacataaga atatcctgtt tgtgacaaac acgactcatg
    16681 aaagtttgta tttcgaacga tatttcggtt atcaaaaagc aatgatgatg gctggtcttc
    16741 ctgcgttatc cagtgtagta ctagagcatc cggaggatta tctgtccttt gagaaaacgt
    16801 tgaaagaaaa cgaagccaca gcattggtgg tcattgacga tatcttcgcc cttcgcacga
    16861 tgcagttggc gcaaatgtat ggctatgtca ttccagagac actctccgtc attagcttca
    16921 acaactcgat cttctcaaca ttaacacacc cttaccttac aagtatcgat atcgatatct
    16981 ccgaattagg acgtgtcgct atgcaaaagc tgcgcgaatt gatccatgaa gaattgagca
    17041 gtggcgtacg tttggtcatc cctcatcgct tgatcaaaag agagacagtc gttcctttag
    17101 aaaacccata ctaaatagct gactcactaa atacgctgac catttatcct atggtcagct
    17161 ttttatgtaa aagaagatac tacttctaac acattgttta tctgtaaaaa agagcgtggg
    17221 acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa
    17281 gcaactcctt cggaaataag ctgaaattca caaaaattta aaaagcaatt ttcttgagtt
    17341 tcttcttatt tcttggagtt aaacgcttct gtcccagcct cttttttcct tccttcaatt
    17401 ttttacacgt cgacgattca ataaaaaacc gcctaatatt gtaaagacag gtgcaagaat
    17461 cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact ttcatcgttc ccataatgaa
    17521 tgtgccgctg actccccacg gtacgagaga attgacggtc gcacctgaat ccgctagtgt
    17581 tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa gaacgcttga atgtttctcc
    17641 tggaagaatg atggacagat actgttctcc aacgatcaga ttgattccga tacagctcaa
    17701 ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt tcttttaatt cttgaacgat
    17761 ggtatcaacg atcagatatt tgatcagtaa cccacctaat gccaatgcaa gtaaaatcaa
    17821 actagcagaa cctaacatac tcatgatccc gcctcgagaa agcagttcat ctagctttgg
    17881 atcaggtgta tttgcgacat aaccattcat caagatgtct gctgcttttc cgaccgtcaa
    17941 acttttctca tggataaaag aaagaacgag agcagctgtc gatcccgcta gtaatgtagg
    18001 gatagccgga atttttttga ttgccccccc aaataaaata actagtggaa tcaatgtcca
    18061 aggagagatc caaaagctcg ctttcatgtt ttggatcatt tcattgatac tagctagatc
    18121 ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc aacgaaagga cgaatgctgg
    18181 gaaatccgta taacgcatac cagcgatatg ttcgaataga ttaactcctc caatacccgc
    18241 tgccaagttg gtcgtatctg ataaaggcga gcagttattc cctaaaaagg caccggaaac
    18301 gactgcgcct gtagtcattg catcattgaa tccaagtaaa tgaccgatcc ccaaaaaagc
    18361 aattccaatc gtcgaaatcg ttgtaaatga actgccgaca gtcactccga caattgcaca
    18421 aacaagaaat gctgtcggta aaaagaaacg cacagaaaca agtttaaatc cataaaccat
    18481 aatcgtaggg atggttccag ataaaatcca tgaagcaact aagacaccaa tcaaaagaaa
    18541 aatcaggatt ggaatgatcc ctggagtgat tcctttgatg atcccgtcat ggatctcatc
    18601 ccatgtgaac tttttaaatc tgccataaaa caacaaacac atgaaagctg caaggatcgg
    18661 gatatgtggc gagagctgga atccgatgat cagtactcct aggagtgcca acaacacgat
    18721 caatacagcg atactttctt ttaaagaaat atttttattc atcgtcgatc tcctatttca
    18781 aatgaaaagg ccctgccatg aaaaaactat tcacttggca ggacctccta ctaaatcagc
    18841 gacaaaacgt cggctgaaag acttattttt ttatttttct tctaaacttt tttctacctc
    18901 aatcccccaa gaatctttct tgaagtgatt gatgatcaat ggaataacga acatgaccac
    18961 tgtgatagct gctactaata atgcatcact caatgtcgct gtacctaatg tagcaaatac
    19021 gacaacgatg atagagaaga tcagcatgca gctgactacc caagctaatc cgtttccgtt
    19081 tttaccaatt cggtaaggac gttcgaattc tggatgtttc ttacgcattt taagcaatga
    19141 aacagcaatc aaaatgtaca cgatacaata aataacagtt gtagcatttg taagtgtcaa
    19201 gaatacagca cttacgtcat ccattacacc ataaagcaaa gcaaataatg aaataacgat
    19261 tgattgtgtt agaacgacat ttctagatac gccgtatttg ttttctttat ggaatccgaa
    19321 ttttggtgga aggaatcctt cacgagctac ttggatgatt gtttttgatg gtccagtaac
    19381 ccaagctgat aattgtaaaa gcacaccaat aaataccatg aagctgaaga tatttccgat
    19441 cacttctggt aagcctagga tttgacagtc aatcaagata ggctgcgtga tatttgctaa
    19501 ttccattttt ccatctggta cgacatttgc tacaagcatc gcattgatga cattcaataa
    19561 aactaatccg atcaaagcga taaatacgcc ttttgtataa ttttttgttg cgtctttcaa
    19621 acgaggcatg taaacagaac tcatttcgat cccaacaaaa ataaatgtga taccagctaa
    19681 gtatttcaat gaatcaatat gttccaagtc agggaaagct tttgaccatg aaaagtcacc
    19741 tagatagccg tttgagacta gtccgacttt gaaggctgcc aaaacaccta aaacgaacat
    19801 gatgactact gggatatata cccctagcca tacaccgatg ttcccgccga cttttgccat
    19861 atcgaatttc aagttcaaga tcgtgatgat ccagtagata actaaaatac aacctaaaac
    19921 gaaccagtga ttattgccaa gttctacatt accaaatgtg tttcctaaca atggtcctaa
    19981 agtagaagcg accattacca tacctgggaa catttgcacc cataatagcc atgcaacgac
    20041 aaatccccat ttgctgccca gtgctgtttt tacccataac tgaggaccgc cttcttgagg
    20101 aagcatcgtg gaaagctcac cggaaaccat tgatatcgga ccggcaaaaa acaaaactgc
    20161 aaaaattgaa taaaaaatca atgtccaacc gactgcggct aacgtaggaa tactccgaac
    20221 cgttgcacac agtgccattg tcagaccgat aaaggtccca agacccattt tacttacttc
    20281 agatttactc accgttgaca ctctccttga ttttttggat aaaataacgg tgtctttgaa
    20341 gtagtttcag caataaacga aacaattcag cgaatatttg aggggattct ttgagtttat
    20401 tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg gatcgaagag cttaggtctc
    20461 cccttgctct tcagatccaa acatgcgtca gaaacagtta gtattcaagc tgttttctaa
    20521 catgattacc ttgcttattt tgcttcgctt gccaataatt ggtctgcgta gattttttcc
    20581 aatttttctt gaagagctgc tttgattttt tcagcatatt cttcaaagtg ttcttctttg
    20641 ttcatgtatg gtgtcattac gctggcacgc aatacagtta cttttccggc acggttccat
    20701 tcttcatctg agaatcctag ttggttcaca aattgtaatg ggctgttgcc atagtctggg
    20761 atagcaaagt cagtatgtga agttaagaac tcattaccgt aaatgcttcc tttcacgtat
    20821 gaagaataat catatacgtc atggttcaat ttattcattg caactaagtc atcgttgcct
    20881 ttttctttga atacatagtc taccatattg aaatctggat aagttaatgg atgaacttcg
    20941 atttctttgt ctcctacttt aaatgacaag tcattcaaga agttgtagaa acgatgtgca
    21001 ccttcgattg atgcacccat taatttacca tatcctgtga cattcaaagg taagacatgg
    21061 tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg aaccttccaa gatataagcc
    21121 ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat atgtcgcaaa gtaagagata
    21181 acgtctctca tgcggatatc tttgatgacg ataccaccgg cagaatatgg aacatagccc
    21241 attttatgtg ggtcaatcgt tacagattct gcttcttcga ttgctttata tgcactgtgt
    21301 acttcttcta agatataatc tttgttttct gtaaagacat tgtatttgta atgaacatct
    21361 tttaaatctt caaatggaat gaagttgttg tcttcgtcta agaaaatcgc acggccgtat
    21421 ccgccataag cagcatctac gtgtaagtag aagtaaatac cgtctttttc taatacgcga
    21481 cgcaatgcaa cgattttatc gataccgtcg attgctcctt cttctgttga tcctacaaca
    21541 ccgactacac ctaggattgg tgttttttca gctgctagac cacgaacgat tttttcaagt
    21601 tcgttgatgt ccatgcgata gttgtggtca actggcactg gaatcacttg gtctaaaccg
    21661 ataccgatga tgtcggcagc ttttagccat gaatagtgtt tagtttgtgg aaccaaccat
    21721 tttcctaatt tttctaaatg ttttccgcta cgtgctgagt gagctttgat ttcgtcgatt
    21781 ttttctggga cgctgtctaa cagattcatg atttcttctg ttgacaagtt cattaattcc
    21841 caatcgcttt ttccagcaac tagttcaggt gtcacttctt tcattgcaag cggcaatgat
    21901 ttgatgttgc gggcatacca aagtccttct aagtttgcta aagaaccatc agcaacgatg
    21961 tggccccagc catctttata gctcattaat tttgcaaatt ccattcctac ttcttcttcc
    22021 atttggcttg ttgcaggaga agattcatag gcaacgttat taccattcca tagcatagca
    22081 aaattgtacg ctagtaaaga tggcatcaat gtttcagagt tcatatgacc ccagtaacgt
    22141 ccggcattat gccaaggaac ggaatgggta cgcatccgcg ctgaaatttc tgatagaaca
    22201 tcctttgttt tattcacagt gtgttcaaaa ctagcactgc ttttttcttc tggcgtaata
    22261 ataggcatgt cttgaggcat gtagttttga cgccagccta aatgttcatc caccaattca
    22321 ttcaacaaag ctttatagat ttggccgttc tcagctttgt ccccaatgaa tagggcattt
    22381 aggtttagat ctttcgacaa tgattcactc atagtgaaac cctcctaaaa aaatatttaa
    22441 attttgtcga gccctacatt acacctgttc ttctattctg taaactaagt tttggcctga
    22501 tttttaatca tattttaata tgtatcaatt atccgaattg ttcacacttt tgaaataatt
    22561 aaaaaaaacg agggaattta caaatgtaaa ctccctcgca aagattgtat taacgacaac
    22621 cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt gtaatagtat ttcccttttt
    22681 caaggtaaaa acttactaaa ttctaatcaa ttactttggc caaaaagtaa tttttcttac
    22741 cttttcgaat aaccacaaac ttaccatcga attcatcgga tggatttatg acaaaattta
    22801 aatcggtaac gcggtcaccg ttaatactta tagctccgtt tgaaacatct tcacgtgctt
    22861 gacgtttaga tggttcaatt tttgtggaaa caagcagttc cacgatattt tcaggtgtgc
    22921 ttgaaatttc aacgttcggc attttaccga atccttgcgc gatttcttcc gcatttagtt
    22981 ctttaatatt tccagagaac aatgcttgcg ttattttttg cgcttctttc aagtcttctt
    23041 cactatgcac aaatcttgta acttcttctg ccaaacggcg ttgtgcttca cgtttctcag
    23101 gttctgtctc tacttttttc gctaagtctt cgatttcttc ttgagacaag aaagtaaaga
    23161 atttcaagta tttcactaca tcgcgatcat cttgattcaa ccagaattgg tagaactcaa
    23221 atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc tgcagttttt ccgaatttcg
    23281 ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa cgcttctgct tctggtcctt
    23341 ctaattttcg gattaggtct aatccagctg tgatatttcc ccattggtct gctccaccga
    23401 tttgcaattg cacatcgtat gttttatgca gatgcaaaaa gtcgatagat tgcaggattt
    23461 ggtaagtaaa ctctgtaaat gagattccta cttccaaacg gctggcaacg atatcttttg
    23521 ctaacatcgt gttgatgttg aagtttttac cgtagtctct caaaaattca agtaaagaga
    23581 tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt tgcatcttta ccaaacaatt
    23641 ttctcatttg attggaaagt gcgtccacgt tatgttgcac ttgttccatc gtttgcaaca
    23701 cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc tgttccgccg ccaatcagaa
    23761 tatatggatg atggcctgcc aattggaatc ttttcatcat cataaatgga atcaaatgtc
    23821 cgatatgcat cgaatcacca gttggatcaa cgccgcaata aagtgaaatg ctcttttctt
    23881 caacgagctc gcgaagtctt tcttcgtttg tttgctgatt aatggcgtcg cgccaagtta
    23941 attcatcaat aatattcatt ttactgacct ccgtgtagtt tttttatgaa agcaggaacc
    24001 tgctagcata tgtaattaat tatttcttca ggataggata ggaaataaaa aaagtccctt
    24061 ggcatataag ccaagggacg aaatctcgcg gtaccaccca atttgtatgt ctagatatcc
    24121 aacatacctc tcgaacattt aacgctgtcg cgtggcaatc actgccaaag actcctgaaa
    24181 tgtaattcgc tttaaagcat gtactggttt gcagcgacca ccagctttct gaaacattgg
    24241 ctttaacaac tacttcgttt cgatcttcgt cttgttatta ttaatatgtg tctatcatag
    24301 agcgcaactt tttctttgtc aacagaaaaa ggatattttt tttaaattcc tcattttttc
    24361 ttttttgaat ccgctctaat tccgaacatt ttattttcaa ttattgttaa aagaaataac
    24421 tttttctgta ttttttctgc aatttatcga gaagtcactt tactttgcct cttattcttt
    24481 cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa aagcaaggga cgaggtatat
    24541 ggaaaaaaag caaaaaatta ttttaggagc cacttctgct ttaactatcg ttattttaat
    24601 tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa gaaccaatca ttcctgaaaa
    24661 aaaaataaca gaaaaagcaa aagaaccgac agatgataag caacaactag ttactcaagc
    24721 aaaaaatctg gctcaaagct acgattatga ccaagcgatt gcgttattgg aaaaagatag
    24781 taagaacgga tctactgaaa cacaacagct tcttcaaaca ctaaaaaagg agaaagaaca
    24841 actgatcgac tggtctgatc ctactcagat ctctcatgtc tttttccaca gcttgatcgt
    24901 ggacccgcaa aaagccttcc actctcaaca ggcacaagga tacaaagatt atatggtgac
    24961 tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa aatgattttg ttttagttaa
    25021 tttgaatgat ttgatccaaa aagacgaaca aggaaatctt tcctttaacg ggcttgcgct
    25081 acctgaagga aaaaaaccat tggttctttc acaagatgat gtcagctatt atgaatatat
    25141 ggataattcc ggttttccta gccgattggt cgtatcaaat aacaatgaaa tcaaaaatgt
    25201 ctataaagag aaagataaag aagaaatcgg tgactttgac atggttcctc tgatcgatgc
    25261 ctttgtcaag aaacatcctg atttttctta tcgaggcgca aaaggtacct tggccctgac
    25321 aggttacaac ggagttttag gttatcgaac atcaaaatct caatatggag acaatgaaaa
    25381 aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa cagttgaaga aagacggctg
    25441 gagctttgct tcccatacat ggggtcactt gaatatgact caagcttcac tagaagatgt
    25501 aaaaaaagac aatgaattgt ggcagcaaga agttgcacct atcctaggaa aaacaaatat
    25561 tctgatttat ccatttggtg cagacatcag cgactggcag ccttattcag cagaaaatca
    25621 aaaatttgct tacttgaaga gtcaaggttt cgatattttc tgtaatgtcg atgcttctac
    25681 accagcttgg ggacaattag gtacggattt ctatcgaaat gctcggatca atatcgatgg
    25741 cattcgtttt caatctgatt tagacggaaa aaatcctatt cttgatccat tcatcaatgt
    25801 ccatgatgtc tatgacaaag aagcacgcga atctgcttga ttttacgtca tttatgaacc
    25861 aaaaaagccg aactgacatt ttatcgtcag tttcggcttt tttatgcttt atttcttctt
    25921 cggtgcgatt cccttgatca ggacttctgt ccattctgct gtatatggaa gcagttttgc
    25981 gtatatctcc ggcttagaaa ttccttctac cattgcttga aaatagagga tcaagaactc
    26041 atccgaatat ttcaaatcca cttttccttc tttccgccct cgatggaaaa gatcaagcat
    26101 gatactgaaa ctttcttgcg tatacgcatc catcgcgtca gaaaattctt ttttttcttt
    26161 ttttgtatag tatgtcatca aatcgatata aaattgtttg ttcactcgat tgagataacg
    26221 gattttgttt tcactcatgg caatcagtgt ttcttcaaat ggaagatttt ccgcaatcaa
    26281 cgctttcgct tcttgcccta aatcattcat aaatgcaaaa aatacttcat aaatcagccc
    26341 ttccttgctg ccaaaatatt taaaaatcgt cgctttactt acctgagcgt tctcagctac
    26401 cttatccatt gtcactccag cggttcctat gtctgtgttc atcaagtcaa aagctgcttg
    26461 taaaatctct ttctttttct cttgtgctct cttttcaaaa ccgttcattt gtagagcctc
    26521 ccgtaatttt aaacttttat aatatatata gttcaaattt attctgaggt ttattataac
    26581 tatctattga gaaagtatca agctagcaat tccttgttta tgaattcatt gacagaaatt
    26641 tataaaaaga atattatgta ctaaaataat aaaaaaagtt ttataaaaag gtataaaacc
    26701 tttttattaa actatttaat cttgtttagt atatattttt agaaaaagga gtggggtttt
    26761 tatggcagaa atcgtcaaag tcacacattt gaaaaagagc tttggtcgtt tcgaagcttt
    26821 aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt ggatttatcg gaccaaatgg
    26881 ttctggaaaa tccacgacga ttcggacact tttagggatt ataaaaaaag acggcggaac
    26941 ggcaacgatt tttgggcaag atgtatggga agatagttta tctatccatg aacgtctttc
    27001 ttatgttcca ggcgatatct ctttgtgggg aaatttgact ggaggagcga tcatcgatct
    27061 cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa gatgaactga ttcaacgttt
    27121 ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa ggaaatcgcc aaaaagtggg
    27181 cttgattgct gctttggctg ttgattcgga tctctattta tttgatgagc ctacttctgg
    27241 tttagatcct ttgatggaaa cgattttcca agaagaagtt gaaaaattga aagaacaggg
    27301 caaatccatc ctactttctt ctcatattct cagtgaagtc gagcgactag cggataaagt
    27361 cgtgatcata cgcgaaggaa cactcgatga agaaggaaca ctcgatgaat tgcgtcattt
    27421 gactcgttcg accatcacta tagaaacaga agcagagaat agtcacttag cacaattgaa
    27481 cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa tttgctgctg ataacgaagc
    27541 tttcgatgtg attttagctg aagcgtccaa attaggtgtg aaaaaatttg aatctattcc
    27601 accgactctt gaggatttat tcatgagaca ttatgaagga tgaggaggaa gcgaaatgac
    27661 tgataaattt tcacgctggt ccaccctttt ttctcaaaac cttagccgtg actggaaaaa
    27721 gatcctgata tggctagtcg gtgtcactgc tttttctggt ggttttgtcc ctgcgtttga
    27781 agaattgact tccggacaag gattagctgg attatacgaa accttgcaaa acccggcaat
    27841 gattgcaatg gtcggaccaa ctccagcttc gaatgctgct gagtatactt taggagcgat
    27901 gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct gctgtgatct ctgttttaca
    27961 cgtggtgagc caaacgagaa aaaaagaaga acttggtctg attgaactta ttcgttcctt
    28021 tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct gaaagtattc tgatcaattt
    28081 gcttttagca atatcgatca gctgtacgat gatttttttc caagcggatt cgatcactgc
    28141 agaaggtgct tggctttttg ggctatctgt aggtagtatg gggattttgg ggagtgccgt
    28201 tgcactgttt gctgcacaac tgatgccaag ttcagccagt tcgaatggtc tgtctttagc
    28261 gtttttaggt ggactttacc ttctacgagc aataacagat gtagctgatt cctccctttc
    28321 gatgtggaat cctttaggct ggtcttattt gacctatcca tttacaacta acaactggat
    28381 tcctttgctt tatttgtttt tgttcagtct aatcttgata atcagttcat ttgttttaga
    28441 ggaacatcga gatttgaaca ccggctatat ccccgaacgt gaaggaaaac cgtatgctaa
    28501 acgttctctt ttatccttgc gtggtttatt attctatcta aataaaggga tgacaatcag
    28561 ttggcttatc acttttggtt tgatgggggc ggcatacggc tctatctacg ggaatatgca
    28621 acagttttta gaaagtaacg acttaatgaa acagatgttt atccaatctg gcgtctcaat
    28681 tgaaaaaaac tttacaagca cgatcatggt agtcttgatt tgtctagctg ctattttacc
    28741 aattgctagc atcaatcgtt tatttacaga agaaacccac ggacgtatga atcagctgtt
    28801 cgcaacaaaa atttctagga accaacttta ttggaccaat atgttactag ctattctatt
    28861 tgctatattg gcaatcctga ttagttctgg tgcactaggg ctttctgctt tggcttcgat
    28921 gaaggatgcg aaagacatgg atctattgat gtttcttaaa gccgggttca atttcctgcc
    28981 tgccgtttta gtgtttatcg gaattagtgg ctttctattc ggatggcttc ctcgaatcgg
    29041 taaacttgct tatatctact tagcttactc ctttgcactc aattactttg gcggaatttt
    29101 ggatttacca gaatggtttt ccaaaacagc tgtccctagc tgggttccac ggatgccaaa
    29161 cgattctttt gatctttccg tgttccttac gttatcatgt atcagccttg cactgttcgt
    29221 ccttggttct attggttata ggcgaagaga cttactagaa ggataataaa aaagcatgta
    29281 caatttcatg ctagcctcca aatctgggtc aaaaatccaa catttggagg tcattttttt
    29341 atttacaagt cttctaactg ctctttatca atgatccaga cttctcgatt ctttcttttt
    29401 acataaccat tctcctctaa taatttaaat tttctagata aagtttcggg ggtcgtaccg
    29461 ataaatgcag caagttcttt cattttcatt ggcaggaaaa agtgatcctt ccctgtcgct
    29521 ttagctaagt caagcagata gagagcgact cgttcttcta ctttttccat catcaaaaaa
    29581 gttgtctgct gctgtacttt tgttaatttc tcaacgttga ttgctaaaag tttcagacta
    29641 agggcgggat aatgcagtaa gagttccgag aaatcttttt gtctcaatat gcaaatttcc
    29701 gttttttcta gtgcttcgcc aaataaagac tcatttggaa cgcctagcaa ctggctctct
    29761 ccctcatatc cgcccggctc tattactcgt aaaagctgtt cctttccgct agcagataat
    29821 tgatacactt tcatactgcc ggaggccacg atcactaatt gaggttctcc attcggcgca
    29881 agaatctgtt ctcctttttc cacatagtga tgtttgacaa gtgcgtgtac tttttcttga
    29941 tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa cacataaatg atcatgactc
    30001 ataatctctc ctccttctaa acagctattt ctaataaaga aacataactt cccttattcc
    30061 aactgataat tttcttttat catagcataa acgtcaagcg aaccagatac cagcaaatag
    30121 agtggatttc tttttgtccc ttgctttatt ttccttcttt tgagacctta agtaattgat
    30181 tcctatcaat tttttaaata tttacttttt gaataatcaa aaacaaacct tcactcgcaa
    30241 gccgagagta aggtttgttt ttcgcattgc ttatttcgtg ttgtctattg aaatttttcc
    30301 gcacctagct tttgatatag tggtgctagt tcttgtaagg tttcagcggt tatttgctca
    30361 acgtgttcga tttcttgcgg atagaagaat agtttaccca tttgaaaggt tcggtctctc
    30421 atcgcttttt tcaactcttc ttgtttcttt gtatcggaca aatagtcaga caaagctaag
    30481 tggtcgtcaa gttcatgttc tggttgcggc catatgtaat aatccttgat atcgactgtt
    30541 ttgctccact ctttcagtat cgcaagcaac tggttatacg ctgcaatgct accatatcgt
    30601 tcttcgcttt tataatgttg gtacatcaaa tagatttggt attgtttttg attcaacaaa
    30661 actgctagac aggcattctc gtcttgacga gattcgcttc ggtacgcgct ccaaaaatga
    30721 ctgcgtaaat tccagccatt cgtccagctt tcgattttag gtttgctcat gccgtacata
    30781 tcaggcaaaa gtgcagctgt ctggagctga atctctttcc atttttgcca gtgttcttta
    30841 tattctttct tgatttgttc aatcttatct gttggatggc tctctttgag ctgtttgaat
    30901 gcgtagattg gtcgatcaaa caactgataa tctgattttc caagttccat tgtttcgcct
    30961 ctttccgtta tcttttgtat tctacctgaa ttttgcgata tggacaaata gaggttgtga
    31021 aaaagctgtt ctgcatcaag tcataagaac agccaaacaa aaaactgcga tgagcatcac
    31081 cgcagtttt
//
LOCUS       DAYYGS010000028        29946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000028 DAYYGS010000000
VERSION     DAYYGS010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.028"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(61..2424)
                     /locus_tag="ACHA44_001168"
     CDS             complement(61..2424)
                     /locus_tag="ACHA44_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HFM8904475.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2589..3093
                     /locus_tag="ACHA44_001169"
                     /pseudo
     CDS             2589..3093
                     /locus_tag="ACHA44_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035543.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA44_001170"
     CDS             complement(3201..3548)
                     /gene="rbfA"
                     /locus_tag="ACHA44_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFM8904476.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA44_001171"
     CDS             complement(3575..5881)
                     /gene="infB"
                     /locus_tag="ACHA44_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFM8904477.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(5894..6205)
                     /locus_tag="ACHA44_001172"
     CDS             complement(5894..6205)
                     /locus_tag="ACHA44_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HFM8904478.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(6202..6495)
                     /locus_tag="ACHA44_001173"
     CDS             complement(6202..6495)
                     /locus_tag="ACHA44_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HFM8904479.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA44_001174"
     CDS             complement(6517..7692)
                     /gene="nusA"
                     /locus_tag="ACHA44_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFM8904480.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA44_001175"
     CDS             complement(7716..8189)
                     /gene="rimP"
                     /locus_tag="ACHA44_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFM8904481.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(8333..12685)
                     /locus_tag="ACHA44_001176"
     CDS             complement(8333..12685)
                     /locus_tag="ACHA44_001176"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFM8904482.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(12897..14606)
                     /locus_tag="ACHA44_001177"
     CDS             complement(12897..14606)
                     /locus_tag="ACHA44_001177"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFM8904483.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA44_001178"
     CDS             complement(14674..15942)
                     /gene="rseP"
                     /locus_tag="ACHA44_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFM8904484.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(16103..16903)
                     /locus_tag="ACHA44_001179"
     CDS             complement(16103..16903)
                     /locus_tag="ACHA44_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFM8904485.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(16900..17712)
                     /locus_tag="ACHA44_001180"
     CDS             complement(16900..17712)
                     /locus_tag="ACHA44_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFM8904486.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA44_001181"
     CDS             complement(17907..18464)
                     /gene="frr"
                     /locus_tag="ACHA44_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFM8904487.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA44_001182"
     CDS             complement(18467..19189)
                     /gene="pyrH"
                     /locus_tag="ACHA44_001182"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFM8904488.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA44_001183"
     CDS             complement(19325..20206)
                     /gene="tsf"
                     /locus_tag="ACHA44_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFM8904489.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIEYDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA44_001184"
     CDS             complement(20305..21087)
                     /gene="rpsB"
                     /locus_tag="ACHA44_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFM8904490.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            21446..21925
                     /locus_tag="ACHA44_001185"
     CDS             21446..21925
                     /locus_tag="ACHA44_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8904491.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA44_001186"
                     /pseudo
     CDS             22142..23233
                     /gene="phnW"
                     /locus_tag="ACHA44_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
     gene            23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA44_001187"
     CDS             23226..24011
                     /gene="phnX"
                     /locus_tag="ACHA44_001187"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050194 - phosphonoacetaldehyde hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HFM8904492.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA44_001188"
     CDS             complement(24357..26048)
                     /gene="argS"
                     /locus_tag="ACHA44_001188"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFM8904493.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA44_001189"
     CDS             complement(26470..27417)
                     /gene="arcC"
                     /locus_tag="ACHA44_001189"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFM8904494.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA44_001190"
     CDS             complement(27532..28551)
                     /gene="argF"
                     /locus_tag="ACHA44_001190"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFM8904495.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA44_001191"
     CDS             complement(28642..29871)
                     /gene="arcA"
                     /locus_tag="ACHA44_001191"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFM8904496.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         7982 a         6445 c         5338 g        10181 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaaatc ccgcaacccc tgatttcaca
     3181 ggatttgcgg gattttttga ttagtctttg ttcaagtcgc gaagcaattc atcgatatgg
     3241 ttcccataga caactgattc atcaagttca aagaacaatt caggtgtctt ataaatactt
     3301 agattgtgcc ctaactctcg acgaatcaat cctgatgctt tattcaatcc atcttgtgct
     3361 tttttcttat ctgatgctaa gtcagataaa atgctgtaat agatcgttgc ttgctgtaag
     3421 tcccccgtaa ctttcacatc tgtgatcgtt acgttctcca cacgagggtc acggaccttt
     3481 ttgcgcaaaa tatcattgac ttcttttaag atttcttgac cgactcggcg atcacggtag
     3541 ttagccataa tgattggcct ccttttatca gtttttagtc atttttgact tcttccatga
     3601 taaatccttc aattacgtca tctactttga tgtcattgaa tttttcaatc atcgcaccac
     3661 attcataacc cattttgact tctttcacgt catctttgaa tcgtttcaga cttgcaagtt
     3721 ggccttcata gattacgatt ccgtcacgga ttacacggac accgctatct ctacgaatat
     3781 atccttctgt tacataggca cctgcaatcg taccaacttt agaaactttg aaggtttcac
     3841 gaacgatcat ttgaccggtg attttttctt caaattcagg gtctagcatc cctttcatcg
     3901 cagtttcgat ttcttcgatg gctttataga tgatacggtg aagacgaata tcgacttctt
     3961 cagtatcagc ttgcactttt gcctgcggtg taggacgaac gttgaaaccg atgataatag
     4021 cgttgctggc tgctgctaag gttacatcac tttcattgat tgctccgaca gctgaatgga
     4081 cgatttttac acggacacct tccacatcaa tcttcttcaa tgatgcagcc agtgcttctg
     4141 ctgaaccttg tacatctgct ttgatgatta cgttaacttc tttcaattcg ccttctttca
     4201 ggctttcaaa taagttgtcc aaagtcacac ggcttgtggc agcacggttt tccaataatg
     4261 cacgttttgc gcgttcttca cccgctgcac gtgctgtctt ttcgtcttcg aagactacga
     4321 aacggtcacc tgcttgaggt acatcattca agccagtgat ttctactggc gttgcaggtc
     4381 ctgcttcttt atcgcgacgg ccaagatcgt ttgtcattac acggacacgt ccgtaagtat
     4441 taccgacaac gattgggtca ccaacgtgaa gtgttccttc ttggacaagt aaggttgaca
     4501 caggaccttt acctttatca agacgtgctt cgatcactgt tccgattgcg cgttgtgttg
     4561 gatctgcttt taaatcttct acttctgcga ctaacaggat catttccagc aattcttcga
     4621 tgttttggcc gaatttcgct gagatctcaa caaaaatcgt ttctccaccc catgcttcag
     4681 gaattaattc atactcactt aattcttgca taacatgctg aggatttgca cctggtttat
     4741 caattttatt tacagcgacg atgattggaa cgcctgcagc tttggcatgg ttgattgctt
     4801 caactgtttg tggcatgacg ccatcatctg cagcaacaac taagatcgtg atatctgtga
     4861 tacttgctcc acgtgcacgc atacttgtaa aggccgcatg tcctggtgta tccaagaaag
     4921 tgattggttt accatcaata tcgatctggt aggcaccgat atgctgtgtg attccgccgg
     4981 cttcgccgct tgttacacgt gaatggcgca atgtatccaa taaggttgtt ttcccgtggt
     5041 ctacgtgtcc cataatcgtt actactggcg gacgtgagac taatttatct ggatcgactt
     5101 cttctgcctc gaagaattta tcgatatcag caatgtcaac ttggactttt tcttgtggtt
     5161 ccatgccata gtccgtcgct aacagttcaa tcgtatcttt atccagtgct tggttttgat
     5221 tgaccatgac accaagcata aataattttt taatgatttc tgctggctca cgatgaattt
     5281 ttttcgcaat atctgctacg ttcatacctt cagtatactc taatacatct ggcaattcac
     5341 ggaatttgcg tggtggtaca gctggtttag ttgattgttg ctgtttgcct tttttacctt
     5401 ttttattata acgattacgg ttattattat taaatttgcc gcgattgtta ttgtttggtt
     5461 tgttgttatt gttagtacgg ttttgattgc cgccttggct cgttccttga cgattttggc
     5521 tgctggtatt tcccgtatta ttttggtttg ttgttggttt ttttccttga ttcacttgac
     5581 cgctcccttg accgcgatct tgataactgc ggttattttt atttttttgt tgattgttcg
     5641 ttgtttcttt tttacgttga tcgttcggct gttgattggc tggttttctg cttgcttgtt
     5701 gtgcagcttg ttgtttcgtg cttgggtttt tgaacgcttg ttgcaatttc ttctcatcac
     5761 tgctagtaat cgcacccata tggtttttca catcgatgcc aagttgttgt gctttttcca
     5821 cgacgtcttt actagatttt ttgagttctt ttgctaattc atagattctt ttgttgccca
     5881 tgcaatcacc ttcctaacct tcaattagct ccttggtctt cttcgcaaat ccggcatcca
     5941 ttattccgaa taccttgcga ggttttccga tcatctgcgt gatttctgct tcagaaaata
     6001 gctcaaagca aggaacttca taatatgaac tcttatcttt tatctttttt cttgtattct
     6061 cacttgcatc ggttgccaca aacaccaatt ttgctttttg tttgcggatt tcagcaatgg
     6121 tcagttcttc acccgtgatt agttttcccg cgcgcatcgc tagaccgatg agattcatgg
     6181 ctttttgccg attaactgta ttcattttcc aaataactct ttacgtgctt tttggtgagt
     6241 gacatagtca agaagttctt gataaaactc atctgttaat gttgtttcga gtactcgatc
     6301 gagaattttt ttatcccaag cttgttgtac ttcttccggc tcaagtgaga catacgctcc
     6361 tcgtccaggc atttttccag ttggatcgat cgatacttcc ccttcttttg aacgggtgat
     6421 tcggaccatc tcttttttag gtttcatttc acctgaaacc acggatttgc gtaaaggaat
     6481 tttcctttgt ttcatctctt tgcctcctta ctaaagttaa gcttctggtt tttcttcgac
     6541 cgtttcattg ttgaatgcaa ttgtttcata ttcatcgtct gttaaatctg actgaacgat
     6601 tgcttcatca tgcagttctt ctgtttcttt ttgttgttct tgattttcat aaaattccgt
     6661 catgtcagat tctgatttga tatcgatttt atggttagtc aatttagcag ctaaacgtgc
     6721 attttgtccg cgtttaccga ttgctagcga taattggtag tctggaacga caactgtgca
     6781 ggcctttgga tttgtctcat cgaagatcac atccatgact tgtgcaggat tcaatgcatt
     6841 tgcgatgaat acagctggat cttcattcca ttcgacgata tccatgtttt cgccttttaa
     6901 ttcattgacg attgcttgaa ctcgttgtcc ctttggcccg acacatgtac cgactgcatc
     6961 gatatttgga tcatttgagc gaactgccac ttttgaacga tcgccagctt cacgggcgat
     7021 gctgacaatt tcaacgattc catcatagac ttctggtact tcttgttcaa ataagcgacg
     7081 caataggtct ggatggctgc ggctaacaaa tacttgcgga cctttagacg tattttctac
     7141 tcgggaaaca taaactttga ttcgatcatg tggttgataa aattcatttg gcatttgatc
     7201 ttgtttagaa agaactgcct caattttacc aagattgaca taaatataac gtttatcctg
     7261 gcgttcgacg attccttgca tgatgtcttt ttcataagca ctgaattcgt tataaataat
     7321 cgttctttct gcttcacgta cacgctgtaa aataacttgt ttagccgttt gcgcagcgat
     7381 ccgtccaaaa tcttttggtg ttacttcaaa acggatcgta tcaccgatct catatgctgg
     7441 attgattaaa agtgcatctt tcaatgagac ttccaactgt gaatccatca cttcttccgt
     7501 tacttctttt accgcataga catggatctt tccttttttc tgatcaaatt ccacttcgac
     7561 gttttgtgct tggccatagt gacgtttgta tgcagaaacc aatgctgctt ctaaagcatc
     7621 gatcacgatt tcttttgaga tccctttttc agcttctaaa gcgtctaacg catttaacat
     7681 ttctttactc atttttttct gattcctctc cgtgattaaa attggatagc taagcgagct
     7741 tttgcaatat tttttcggtc aaaagtcaat tctttttctc gtgtttttat gcgtattttc
     7801 aatgtaagct gttcagcatc aaatgattga agaaatcctt catattgttt ttcgccatct
     7861 actggttggt ataaggagac atggacataa tcaccaagtg ccttttcata gtcgctttct
     7921 tttttcaatg gccgctctgc tcccggggag gatacctcca ggaaataagc ttgaggaatc
     7981 ggatctggat ctgctgcatc aagtttttca ctcagtttct cactgacaaa ggcgcattct
     8041 tcgatatcga taccgccttc tttgtcaata aatactcgta agaaccagct ttttccttct
     8101 tttacaaatt cgacttcgac aagttcaaaa ttttgttcat ctaaaatcgg tgtcaccatt
     8161 tctgtgactg tttcaacgac gctactcaaa agtttcgcct ccttaaaaat taagtgcgta
     8221 attccattaa aaagagtgag cgaaaaatcc gctcactcac agttagtatc attacctata
     8281 agaactatac catactctgc ttaaaattac aagcagacag gaaaacagat gcttatagca
     8341 tatcgaataa cgataattga ttttcatctg gtagatcttt caatacgcca ttgtcgttca
     8401 tatattcgat caatgttttt gaaactttac cgcgggtggc aagatcttct ttggataaaa
     8461 atggaccgtc ttttcttgct tcaacgattt gtttcgccac gttggttccc aaacttggga
     8521 cagctctaaa tggtgcgatc aacgtatcac cttcaataac aaagttaacg gcatccgatt
     8581 tatacaaatc aatcattcca aatttgaatc ctcgttccag catttcattg gctaactcca
     8641 gcactgtcag ttgattcttt tcttttacgg aagcatctaa tcccttgtcc gtgatttctt
     8701 tcattgcttg tttcacagca tcttttcctt tgcacatgga gactagatca aagtcgtctg
     8761 cacgtacaga aaagtatgca cagtaataca aaatagggaa ataaaccttg aaataagcga
     8821 cacgaagagc catcaatacg taagctgcag catgggcttt cggaaacata tatttgattt
     8881 ttgaacaaga atcgatatac cagtcgggca cattattttc tttcatcgcc gatagatagg
     8941 tttctcgcaa ttcatcaggg atcttgttcc attgtccttt acgaacagtt tccatgattt
     9001 taaatgccat cccgctatct aatccagcat ggatcaaata aaccataata tcatcacgac
     9061 agccgattac ttccgccagt gtcgcatctc ctcgtcgaat caattcttcc gcatttccta
     9121 accatacgtc tgtaccatga gatagtccgg aaatttgcag tagttctgca aaagttgtag
     9181 gatgcgtttc ttctaacatc cctcgaacaa aacgagtgcc gaattctgga atacctaatg
     9241 tccctgtttt ggaataaatt tgttcctgac ttactccaag tacttcagga ccggcaaata
     9301 tccgcatcac ttccgggtca tccgtaggga tcgtttgtgg gtcgatccct gataaatctt
     9361 gaagcatccg aataacagtt gggtcatcat gccctagaat atctaatttc aatacattat
     9421 cgtgaatcga gtggaaatca aaatgagtcg ttttccattc tgaattttga tcgtctgctg
     9481 gatattggat cggcgtaaaa tcatagacat ccatataatc gggaataacg atgatccccc
     9541 ctggatgctg gccggttgtc cgtttgactc cagtagcgcc ttttgctaat ctgtcgactt
     9601 ctgcacttcg aaactgcaga ttattgtccc tttcgtatcc ttttacatag ccatatgctg
     9661 ttttgtcggc gactgtacca atcgttccgg cacggtaaac atagtcttcg ccaaacaata
     9721 ctttcgtata gttatgggct tctgcttgat aatcacctga gaagttcaaa tcaatatcgg
     9781 gcactttgtc tccatggaat cctaaaaatg tctcaaatgg aatatcatga ccgtctttgt
     9841 ttaagcgtgc accacatttt ggacattgtt tttctggcat gtcaaatcca gaaccatacg
     9901 taccatcttc aaaaaactcg gaatattggc actctggaca ataataatga ggggctaatg
     9961 gattaacttc agtgattcct gtcatcgtcg caacaaagct agagccaaca gagccacgtg
    10021 aacctactag atagccatct tcattacttt tatgcactag tttttgggcg atcagataaa
    10081 taaccgagaa gccattacca ttaatagaat tcaactcttt ttctaatcgt ttttgaatga
    10141 tctcaggtaa cggatctccg tacatttgtt ttgcttttgt gtaactcaat tctgatattt
    10201 catcttctga acctggaatc ttcggtgtat aaagttcatc tttgactgga atcacttctt
    10261 cgcagatatc tgcgatttta ttcgtatttt ctactacgat ctctttcgct gtttcttccc
    10321 ctaaaaagtg aaatgcagtt agcatctcat cggttgttcg gaaatggaca tccggcaaac
    10381 tatgacgatt caacggattc gctccgccca tagaattgat caaaattttt cggtaaatgg
    10441 catcttcttc gttcagataa tgtacattac ctgttgcaac aacgatttta tccaaacttt
    10501 ttccgatttc taccaaattt tggataattt cttccaaatc atgttcattt tttaccaatt
    10561 cttgctcgat caaaggcgca tagaccgctt ttggcataac ttcaatataa tcatagaatt
    10621 ttgctcggtt tcttgcctct tctacccctt tttgcatcat ggcttcgaat atttctccct
    10681 tatcacaagc tgagccgatc aatagatttt cacgcatctt tttcaattgg gagcgtggta
    10741 ttcgtgggac gcgttcgaag tattcgacat tcgacattga aatcagtttg aacaaatctt
    10801 tcagtcctgc ctgatttttt gctaaaatcg ttacatggaa aggtcgagcc cttttgtaag
    10861 aatctccttc tccgatatgc tcatttagct gatcatgata aagcatgtta tgattgtcca
    10921 ttgcctcttt cacaaagatc catgctaagt gaccagttgc ttcagcatca tagattgctc
    10981 ggtgatgctg ttccaagcta acaccaaact ttttagataa cacacctaat ccgaaacgtt
    11041 tgaattgagg atacagatat ctagctaatt ccaacgtatc aatgactgga ttggccgctt
    11101 caggaatacc ataccttgcg tagctagtat tcaagaaccc catatcaaaa gccgcgttgt
    11161 gggctaccaa aattgtatct tttgaaaatt caaggaacat ccgcaatact tcttcttccg
    11221 actttgaccc tcgaaccatt tcatccgtga taccagtcag atctaccgtt gttcgtgaca
    11281 atggatgtcc tggatcgata aattcatcaa agctttcgat cacatttcct ttatacattt
    11341 tgactgcggc tagctcgatg atcgtgtcat atactgcaga caatcctgtc gtttccacgt
    11401 caaatacgac gtatgtggct tcactcaatg cttcatgtgc atcattataa gcaattggga
    11461 caccatcatc tacgacattc gcttccacgc cgtataagat tttcacacct gctttttttc
    11521 cagcactatg tgcttccgga aaggcttgcg caccaccatg gtctgtaatg gcgatagctc
    11581 ggtgtcccca ttttcctgct tgtgccacaa gatcagagat gctgtttgtt gcatccatcg
    11641 tactcatatt gctatgaaca tgaagctcta ctcgtttttc cccttcagga gcataatctt
    11701 tgcgaggtgt atgtttgact tctatgatat cttgcgcatt catgaccaaa tcacggacaa
    11761 acgtatcttc ttgaatactt cctcgtacct ttaaccagct accgacactg atcgcatcaa
    11821 atacttgttc atctttttcg ccgttcgaga attttttgac gataaatgat gaagtataat
    11881 ctgtgatttt caaagtcaag atcttgcgtt ttgaacgtag ttctctgact tctttgtcga
    11941 atacgtaccc ctcaagtgtg actcttcgtt cttcttcaac gatactgatc attggtgtcg
    12001 ttggttcatc ggctggaata tttcgtccta gctggatggg tccttcaaga gctggcgatt
    12061 gctcttttct ttcctttttc ttttgttcgt ggacaactag actttcagct gcttgcttca
    12121 taaaggcttc tgcctgttct tgtttgcgtt cttcaaaaag tttcaataca cgttccgctt
    12181 gctgctcatc cacttctggt tcgatacgaa attttgggaa tccgtaactg acgaacaact
    12241 cttctactaa tggcaagtat tgttgcttca aataagaaat agctcctgta ctgtcgattg
    12301 gcaagatgac tttacgatct tttatcacag gtacctgcgt tttcaatact tgctgtgcta
    12361 gtggtgtatc gcattgctga ttttccaaag cttgggccca gtaatcttgc aagagctgtt
    12421 catcgaatgt ctggtcatct gctaagatat tcagtttgac ttgtgcaata tctttaaaag
    12481 cgatagtcag atgctgagtc aatgaccgat atagcataat aggcaaaatt ttggaaaaat
    12541 gtagcgtaaa ttcccataaa cgcgatttac gatggaccac tacactatcc atttcaccag
    12601 cagtaagcag tggatgattt ttttctgctt cctctagttg gatttgttcc aacagttttt
    12661 caaataaatc tcgtttttct gacaaagata tacgcccttt ctgatgagaa acaatgcaat
    12721 tgatcgcatc ttatcctctt ctttttcagt accttttagt atactcgttt tttctgagtt
    12781 ttacattaaa aaattggaac tgcgattcaa atcgcagttc caattttaaa agcgaatcat
    12841 cagattgtca aaaacagctg attcagctat ttttattttc tgtgttttaa cgcttattat
    12901 tcgccttggt tcaagaggat tggtaaagta ttcgccaatt cttctttacg tacttcaacc
    12961 atttcgccag ttttcttgat tttcacttct acgattccat ccactgcttt tttaccgaca
    13021 gtgattctga ttggacagcc aatcaaatca gaatccgcga atttcactcc tgcgcgttca
    13081 tttctgtcat caaccagtac ttcatagcca gcttttgtca tcgcttcttc tagttcattc
    13141 gtcaggcttg tttggtattc gtcttttaga ttcatttgga cgatatgcag atcaaatggc
    13201 gcaattcctt ttggccaatt gattccttgt tcatctgaat gttgttctac gattgctgat
    13261 aataaacgac ttacaccgat accataacag cccataatca caaatttttc tcgtccattt
    13321 tcgtctagta cagttgctcc catagattca ctgtaacgag tgccgatttt gaatatatgt
    13381 ccgatttcga ttcctcgagt aaaggcaaga acaccgttgt tgtctggtga aggatctcct
    13441 tcttgtacaa aacgaagatc ttcataagaa agcacagtga aatcgcgttt tggattcaca
    13501 tttttatagt ggaatcctgt ttcgttcgct ccagtaatgg cattcgctaa atcttggaca
    13561 taacggtctg catatacttt tacgtcatct gaaacaccga ctggtccgac agaaccaaac
    13621 tcagcaccta gatatttttg tgcttcttct tctgttgctt cttctaaaaa gtcagcagat
    13681 aaaaagtttt tcagttttac gtcattgact tcatggtctc cacgtaccaa aacaaggaca
    13741 ggttgttcat ccgcaatgaa caatacagac ttgatgattt tttgaggttc aacagaaaag
    13801 aaatctgcta cttcgtcaat tgattttacg ttaggtgtag ctactttctc caattcaagc
    13861 tgtgtttcat gcgatttttt tgatacatag tagcttgtag ccatctccaa gtttgcagca
    13921 taatcacttt cagtcgaata acagatcgta tcctccccga tttctgagat cgccatgaat
    13981 tcctttgagt ctttcccgcc cattgcgccg ccgtctccga tgatcgagcg aaattctaag
    14041 ccacaacgct tgaatacttc ggtataggct ttttcgtaat ctttatatgt ctcgtccaaa
    14101 ctttcttccg ttgcatggaa agaatatgca tctttcatga taaactctct tccgcgaagc
    14161 aaaccgaagc gaggtcgttt ttcgtcacga tatttaggtt ggatttgata aagatttaaa
    14221 ggtaatttct tatatgaatt gatttcatta cgcatcaaat cagtaaaagt ttcttcgtga
    14281 gttggtccaa ggatcatttt tcgatcattg cggtctttga aacgatacaa gtttggtcca
    14341 tatgtttcgt aacgcccgga ttcttcccaa agttctgctg gcaagatggc aggcatcagc
    14401 atttctaccg ctccgatttt ttcaaattct tcccgcataa tgcgtttcag tttttccaat
    14461 acacgatttg ctaatggcag atatgaataa atacccgctg atacttggcg gatatatcca
    14521 gctcttagca agatttgatg gcttaatact tctgcatcat ttggaacttc gcgtaatgtt
    14581 ggaatcaaca tttttgattg tctcattcta tatactcctt tatttcctca aaatagttat
    14641 cgtttgttat tataccctat ttgtcaactt tttctagaaa aagaatcgtt gaatatcgtt
    14701 ccatgtaact agcaccatca acaacattaa aaatccaaag ccagctaatg tcaatattcc
    14761 ttctttttct tgacttaatg gtttcccgcg aatcccttca aaaatattca agactaattt
    14821 cccgccatct aaggcaggaa tcggaagcag attgacgatt ccgagattca ttgaaagaag
    14881 cgccatcaaa ctaataacag ttgttacacc ttgatttgct gcttctgaag acaattggta
    14941 catcatgaca ggtccgccta atttatctaa actgaaacct gtaaatagtg atcctaaggc
    15001 tttgaaaatt tccaatgaac cgctgaaagc ttgacgcgtc cctccaatga tcttatccat
    15061 aaaacctgta ttcattgggg cttttatacc aagttggcca actttttcgc catttgactc
    15121 tacagatttc ggtgtcacgt cgacagattg cacctgacct tctcgttcga tcttaaagtc
    15181 taatgtttta cctggatttt tcgtaatgac tgttgtcaga tcattccatg aatggatttc
    15241 ttttccgtcc acactgacta cctcatcatt ttcttttaag cctgcttctg ccgcagctcc
    15301 attaggcatg atctctccta cgcgattagt gttcgtcact tgaacaccac cttgcataaa
    15361 tgccagaatg atgaataaca cgattgccaa aataaagtta ttcatgggtc cagcaaagtt
    15421 ggtcagcata cgctgccaca atttagccga ttggaactgt acgtcttttg gcgcaattct
    15481 gatttctgtt ccgtcgtttt caataatcgt cgcatcgtga tcaacagggt aggttactac
    15541 ttctgtttcg tctccattca cataaccagt gatagtcagc tcatcttcta aatcataacg
    15601 gctcaattcc atcgggatag catttgtcaa ttggattttc ttacttaaat ttatcttctc
    15661 tacgatgccg tcagaattta gtaaaagaga aagaggcatt cctggtgcca tttctgtttc
    15721 atcatcccca ttgcctgcca ttcgaacata accgccaata ggtaataggc gaagcgtata
    15781 tgttgtcccg tcttttgctt gatgcccata aattttcgga cccataccga tagcaaattc
    15841 gcggacgagg attcctgatc gtttcgcaaa gaagaaatga ccaaactcat gaacaatcac
    15901 taatatacca aaaacgatga taaatgtcag aatcgttttc atagcttttt tctcctttac
    15961 ttgttttttg tgttttttat aaaacaacga taaaacacta taattttacc atagtatacc
    16021 aaagacacca atagtatcaa tataatgaaa aataaaaacc tgcgtctttt atgaaattca
    16081 caaagaaacg caggtatact tactaaataa tgccaaataa atgcatgata ggaaatacaa
    16141 acagcaaact gtcgaaacgg tctaagattc caccatggcc tggtaagata tttcccgagt
    16201 ctttcacatc ataatgacgt ttgattgcag attcgaccaa atcaccaaat tgtccgactg
    16261 ctgaaaaaat aatcgtcatc agaagcatga caaacatacc atgattgaat agttcttttg
    16321 ctgggtatag caataaataa agaaatgcga ctacaacagc actaccgata ccaccgagcg
    16381 caccttcaat cgttttgttg ggcgaaatat caggacttaa cttatgctga ccataacgtc
    16441 gaccaaccat atatgctccg atatcagttg cccaaacaat aaatagacca aatatcaaaa
    16501 cagccaagct ttctgttcgt gcattgacaa aattctggaa acctattccc acatacaagc
    16561 tgacaagcac aggaaaacct gcttcatcaa tcgtatacgt atttttagaa ataactgatg
    16621 ttcctaaaag aatcataact ataagataaa ataacataaa tgtacttaca ttctctggaa
    16681 gaaagaaaaa ccatctttct tttggcaaaa caagtaacac tgcaccaacg gcggataaaa
    16741 ctccttcaaa acttaagagt gtcaatcctt tcatccggaa cagctcgtaa actcctacta
    16801 ccgctaagat tgctgctgta agctcaaccg ctatgcctcc gatccatata atcggaataa
    16861 acaagactag cgccacagct gctgtgatca cacgttgttt catgactgat cccctttctc
    16921 atcttctgtt ttttttacac cgccgaatcg acggtctcga ttttgataag aggcgattgc
    16981 ttcttcaaga tgcgcaccat caaaatcagg ccaaagcgct tttgtaaaat acagctcact
    17041 atatgcgatc tgccataaca gaaaattact aattcgctct tctccacttg tacggatcat
    17101 cagctcagga tcttgtaact ctttaggcaa gaaacctgtc attaaatgct tagcaatcaa
    17161 ttcatcatcg atttcttctg cgtgtatttc tccttttgct acttcttcgg caatttcttg
    17221 gacagcagtc acgatctctg ctctacttcc ataattcaaa gcaaaattca ataccattcc
    17281 agtattgttt tttgtttgct caattgcacg tcttacagca tcttgcgtat gttctggcaa
    17341 tacgttttca taacccatga catgaacttt gacattttct ttaatcaatt ctggtacgaa
    17401 cgtatcaaag aagtcgacag gtagttgcat gagaaaactt acttcatctt ttggtcgctt
    17461 ccagttttca gtcgaaaagg cataaagcgt caacactttc actccaagcc ttgatgcttt
    17521 ttttgtgact ttctttactg tttccatgcc ttctttatgg ccagcaactc gtggcaaacg
    17581 acggttttgc gcccaacgac cattgccgtc catgatgatg gcaatgtgct gaggaattgg
    17641 tccatcggta ttaaattgaa attcgctttt ttcttgaata tatttgtttt tttgcggaaa
    17701 aaaacgtaac attctttttc ctcctatact taaaagattc ccactatctt aagtattata
    17761 gcaatatcta actgtttttc ctatgaaatc attgtttttt tatttaaaaa ctgaataatt
    17821 ttttcttttt ttaattttta aataaaagaa atataaataa aaacagagga agcggaatga
    17881 tccgcccctc tgtttttatg attttattaa acttccaata attctttttc tttatcagca
    17941 gcgatcttat ctacttcttt aatgctgtta tctgtcaatg tttgtacgtc tttttctaat
    18001 ccgcgcaaat catcttcggt aatatcacca tttttttgtt gttttttata ttcatcgatc
    18061 gcatcacgac ggatgttacg aatcgcgatt tttgcatttt ctgcttcttt tttcacatct
    18121 ttagctaatt cttttctgcg ttcttctgtc agttgcggga taactaagcg aatgacattt
    18181 ccatcattgg ttggactgat tccgatatca cttgccataa tagctttttc aacatcttga
    18241 agaatgctct tgtcaaaagg tgtgatcatt aaaacacgtg cttctggaat ttggattgat
    18301 gccatttggt tcaatggtgt tggcgcaccg tagtagttca cagtaatacg gtccaaaagg
    18361 ctagcgttcg cacggcctgc gcggatttgc ccaagttcac gttgaagatt ttgtgctgct
    18421 tttttcattt tttcttttgc ttctgtcata attgcatctg ccatttttat ttccccctta
    18481 cagttgttcc gatattttcg cctaagattg cacggcgaat atttccgtgt tcatttaaat
    18541 tgaagacaag cagtggaata tcattatcca tgcttagtga acttgcagtt gaatccatga
    18601 cttgtaatcc tttggcaata acttccagat gagtcagttc ttcaaatttt actgcatttg
    18661 catctacttt cggatcagca gaataaacac cgtctacatt gtttttagcc atcaggataa
    18721 catctgcgcc gatttctgcc gctcgtaatg ctgctgttgt gtctgtcgag aagtacgggt
    18781 tgccggtacc gcctgcaaag ataacgatcc gtcctttttc taaatgacgt tctgctttac
    18841 ggcgaatata tggctctgcg atttgacgca tttcaatcga agtttgcacg cgtgtcggta
    18901 cacccacgtt ttccaacgta tcctgtaaag ctaaggcatt cataactgta gctaacatac
    18961 ccatgtaatc agcttgagca cgttccatgc ccatttgtgc tccgatttgt ccgcgccaga
    19021 tatttccgcc acccacaacg attgccattt caacgcccag ctcgtgaact tctttgattt
    19081 cttggatgat ttctttgatt actggtggtt tgattccaaa tcctgcatcc ccagctaaag
    19141 cttctccact caattttaag actacacgct gatatttcgg tgtgaccatg ttcattcctc
    19201 caatattttc tattgccatt ttaccatact tagatgattt cgacactcta agactggttt
    19261 ttcttgtttt gcttaaaaaa agggagcgca caaaccgtgc gttcccgtct gttattcaac
    19321 agaattattt tttcacttgg ctcataactt cttctacaaa gttatcttcg cgtttttcga
    19381 taccttcgcc aacttcgaaa cgtacgaatg ttttaacagt tgcgcctttt gatgcaacgt
    19441 atttttcaac agtcatatca ggatctttaa cgaatggttg gtccaccaaa gcgatttctg
    19501 ctttgaattt cttcaagcgt ccttctacca ttttttcaac gatattcgct ggtttgcctt
    19561 cgttcaatgc ttgttctgta agaacagttt tttcatgttc taattcagct tctggaattt
    19621 gtgattcgtt tacataacga ggattgatag ctgctacgtg catagctacg tctcttgcaa
    19681 ctgtttcgtc agttgttcca tctagaacag tcaatacggc aatacgtccg cccatatgca
    19741 agtatccacc aaatgcagca ttgtcgtctt tttctacaac ttcaaagcga cggaagctga
    19801 ttttttctcc gataacttga gttgcttcga tcaagtcata ttcaattgta cctttatctg
    19861 ttttgatttt cattgcagct tccatgtctg ctggtttgtt ttcagcaaca agttctgcaa
    19921 tttcttttac taaatcttgg aacatttcgt ttttagaaac aaagtctgtt tctgagttta
    19981 cttcaacgat tgctgcagtg ttacctttca cagcaactga agccaatcct tcagctgcaa
    20041 tgcggtcatt tttcttcgca gcctttgcca ttcctttttc acgtaaaaga tcaactgctt
    20101 tttccatgtc gccttctact tcaactaatg cttttttcgc atccatcatt cctacgccag
    20161 tcatttcgcg tagttctttt accattttag ctgtaacgtc tgccatgttt aactcctcct
    20221 gtgtgttttt ctttttaaaa aaagctgctt caaaggagag tgaggcttgt cgcccagcct
    20281 ttgaaacagc tccatcattc aaaattattc tgctgaagaa ttgtcgcctt caacaacatc
    20341 tacgatttct tcgattgatg ttgctgtgtt ttcttctgtg aaatcttctt caactacttg
    20401 atcttcacct tggtttcctt cgatgaaagc atcagccatt tttgaagtaa tcaatttaac
    20461 ggcacgaatt gcatcgtcgt ttgaagggat tactacgtcg atttcatctg gatcgcagtt
    20521 tgtatcaacc atagctacga ttgggatatt taatttgtgt gcttcttgaa cagcaatacg
    20581 ttctttacgt gggtcaacga tgtacattac atctgggatt cttggcatat cagcgatacc
    20641 gcctaagaat ttttcaagac gttcacgttc tttgttcaaa ccagcaactt cttttttagg
    20701 tagaacttca aaagttccat cttcttccat tgcattgatt tgtttcaaac ggctgatacg
    20761 tttttggata gtatcccagt ttgtcaatgt tccgcctaac caacgatggt taacgaagta
    20821 ttgtcctgca cgagttgctt cttctttgat tgcttcttgt gcttgttttt ttgtacctac
    20881 gaataatgct acgccgcctt cttcagcaac gctcttcatg taatcataag ctgcatctac
    20941 taatttaact gttttttgca agtcaatgat gtagattccg tttctttctg tgaagatata
    21001 tttcttcatt tttggattcc agcgacgagt ttggtgacca aagtgtacgc cggcttctag
    21061 taattgtttc atagaaatta ctgccatgtt tgtttcctcc gatttggttt tatttttcct
    21121 cttcttatct tctaccttgc cggcaaactt tttcaagcac ctaccgacaa tccaataaga
    21181 atgtggattt gctgatgcat aaagcaccct cagctattat acagacaaaa gatccgtatc
    21241 gcaagagcaa atatactttt ctgctcaaca ttcaatagtt tacctgaaag ataaaaagaa
    21301 ttcaagaaaa acagtagatt tctctttgtt tctttattat cctttttcca taaaataatt
    21361 ttaggtataa aagacgaata ttattgggca atccttcttt tttttgttat gatacatgtg
    21421 aaatctttcg taaaggagaa agaaaatgcg caaagctcaa agacatttgc tgattaaaca
    21481 agtcattgaa gaattcccga tccgtacgca agaagaacta ttaaacaaac ttgggcatta
    21541 tggcgttacc gccacgcagg caacgatttc tcgagatatc cgtgatctaa aaatcgtgaa
    21601 ggcacctgat gaaaatggcg tttcccgctt cgtattgttt cgcgaaaaag gaactcctga
    21661 gactaggact gaggaagagg atcggctggt tcaaatgatt gaagacattg tattgaaagt
    21721 cgaacgagtc cactttttga cgatcgtcaa taccttgcca gacaatgctc atttatttgc
    21781 atctgtttta gatgagatca aacctccctc tattgtcagt acaattgctg gattcgatac
    21841 gatcatggtc atctcggaaa atgaggagga cgcaaaacga gtagaagaat ttcttcacca
    21901 tccaactgaa gtatcacttt tttaatcaga gcacgtattc ctaatatgga atgcgtgttt
    21961 ttttattttc aatattttcc ttattaaata aacctttaca ctctttacaa aaatacatca
    22021 ttttttattc aattttttca acaccttgat tgaatcacaa taatcatcat ttatattgga
    22081 tgttaacaag agcagcttct tgctctttgt gtatttcaaa aaagaaatga aaggaaatca
    22141 tatgtataaa ttattaactc ctggaccatt gacgacttct gaaacagtga aaaatgctat
    22201 gctgatcgat cattgtactt gggatcaaga atacaaagaa attacacaat ggataagaaa
    22261 aaaattattg gatcttgctc aagtatcgga aggaatatac acaacggtct taatgcaagg
    22321 tagcggctcc tttggggtag aatcggtatt aagttctgtg atcaggccag aagatactgt
    22381 actgatttgt tcaaacggcg catatggaca acgaatgatc aacatgtgcg aatgtctgaa
    22441 actgaactat accgtttatg aggtatcgta acatcaaatc ctccaagctc atgtgattac
    22501 acagctgatt gaagagaatc cctctatctc cgcactagca atggtccata gcgagactac
    22561 ttccggtatt ttgaataatt tggaagagat tgctgctgtc acacaagaaa aagatctttt
    22621 gtttatagtg gacgcgatgt caagctttgg tgggataccg attcgtgtag ccgaactagg
    22681 gattgatttt ttgatttcca gctcaaacaa atgtattcaa ggtgttccag gcttttcttt
    22741 tgtacttgct aataaagcgg aactagaaaa gcgaaaaaat gtgagtcgaa gtttgtcact
    22801 tgatctatat gaccagtacc gcgtgatgga aaacgagaat ggcaaatggc gtttcacttc
    22861 ccctacccat acggttttgg cttttcaaca agcgctgaag gaactagagg cagaaggagg
    22921 aatcgaaact cgatattcac gttatctcgc caataatcat gtactacgac aaaaaatgac
    22981 agagatgggg ttccagcctt tcattcaaga atatcagggg ccattcatca cctcttttct
    23041 ttaccctaag gactggcgat ttgattttaa tcatttttat cagtatttaa agcaaaacgg
    23101 atttgtgatc tatcccggga aaatcagcga tcatgaatgt tttcgtatcg gaaatattgg
    23161 cgagatctat ccggaagata ttttggaact gacagcaatt atcaaagact ataaggagaa
    23221 cgttaatgat taaaactgta atttttgatt gggcaggaac aactgtggac tttggctgta
    23281 tggctcctgt ccacgctttt agaaatgctt ttctagaaaa aggaatccaa ctgactgaca
    23341 aagaaatccg cgaaccgatg ggaaaattaa aatgggatca tatccaacag cttctttccc
    23401 tcccttctgt caaaaaacaa tggatagcta tttatggaca actacctcag gaaaaagagg
    23461 tggaggaatt gtatcaacaa tttgaacatt atctgtttaa agatttggtc aagcattcta
    23521 ccttgaaacc cgacacattg gaaacgatcg aacggttgag aaacgctggg atcaatattg
    23581 gttccacgac tggatttact gctcctatga tgacaatcgt agcagaaacc gcaaaaaaag
    23641 aagggtattc accagacttc atggcaactt ctgaagacgt cgaagggtat ggtcgaccgt
    23701 ttccttatat gatccataaa aacatgcagt acttcaaaaa caagtcggtt gatgaagtaa
    23761 tcaaagtcgg agatacggtc tcagatatcc aggaagggaa aaatgccgga gtgttgacag
    23821 taggtgtaat tgaaggtagt tcgctaatgg ggatttctta tgatgagtac caaaaattag
    23881 agatggggaa aaaagatcgt ttaaaacagt ctgttgctct tagattccaa caagctggcg
    23941 ctgatatttg tgtaaacaat ttaaaagagt taacagaata cattttgtac gctgacaaga
    24001 atccagaatg acttttatac aaaatcagag gtcgtgaaaa agctgtactg catcaagtaa
    24061 taagaacagc caaacaaaaa tagctcctca tatttttcgt ttggttaggc ttattaccgc
    24121 agatgcaagc ttttgaacac cgtttattcg aaaaaaaaag ggagctgtga cacgactttt
    24181 gtcacagtcc ccttatttac aaacaaacag tgtttaagag acaacctctc cactaaacgg
    24241 tgttcattca gctgactctc ggtatttgct caaaaaacga taaaatttga aaagctattt
    24301 tctcttttgt ttctctaata ctcggatcag aacgctgatt tcacattctc tttttcttac
    24361 atcttatctg gtgcatgaag tcctagcaaa cgaaggtctt ctttcaataa tacagtcact
    24421 gcataaacta gagctaagcg tgcttctttt tgttcatcat cagctaaaat cttcgtatga
    24481 gcatagtatt tgttgaatgc ttgtgctaat ttgattgcat gtttcgcaat cactgacggt
    24541 tcgtattttt ccccagcaga caagaccgtt tcagggtatt tttgaactaa tttaaccact
    24601 tcccagctgg tatcgtcatt caatgcatag tctgctgttt ctgaagggac aaatcctgct
    24661 ttttccaaca ggctgactgc acgagcatgt gtatattgca cataaggtcc agtttctcct
    24721 tcaaatcgaa caacttcttc caggttaaag tcaaatgtat tcagtcgatc gtttttcaaa
    24781 tcgtggaaaa tgactgcccc aaccccaact tgtttagcta cagcatcttt gttttccaaa
    24841 tcagggtttt tctcgctgat ttgttcttta gcagattgga ttgcttcatt taatacttct
    24901 tctaataaga cgatctttcc tttacgagtt gataattttt taccaccttg cgtgatcaaa
    24961 ccaaatggaa tatggcgcat atcgtcagac cagtcaaagc ccatttcctt caagacagct
    25021 ttcaactgtt taaaatgata gctttgttca ttccctacta catatagaga ttgtttgaag
    25081 tcgtaagtac gtttacggta caatgcagca gctaagtcac gtgtaatgta tagtgtcgca
    25141 ccgtctgact tcttaatcaa cgctggattc aggtcataag cagataggtc aacgatctct
    25201 gcacctttat cctcgttcag caaatgtttt tcttctaaaa gtttgacgat ttcatccatc
    25261 ttgtcattgt agaaagcttc accgttcaac gaatcgaaac gtacttccag cagatcatag
    25321 attttattga attctttcat cgattcatca cggaaccatt gccataattg aatagcctct
    25381 tcatcgcctt cttccagacg tttgaaccaa gcacgagctt cttcatttaa ttctggttct
    25441 gtttcagcta cttcatggaa ttggacatat aaacgaagca attcattgat tggttctgct
    25501 ttaacagctt cttctgtacc ccattttttg taagcgacga tcagttttcc aaactgtgtt
    25561 ccccagtcac ctaaatggtt gatgcggatt ggctgataac cgattttttc cataatgaaa
    25621 ccgatcgaat taccgataac tgtcgaacgc aaatgtccca ttgagattgg tttggcaata
    25681 ttaggagaag acatgtcgat tggtactgtt ccttgattcc cgatattgct atccccataa
    25741 tgttcttttt ctttcactac tgtttgcaaa acttttttgc tgataagttc tttattcatg
    25801 aaaaagttca agtaaggtcc gacaacttcg attttttcaa agttggcaga atcgattttt
    25861 tctgctaaat ctgcagcgat ttgctgtggc gcttttcggt aaacttttgc taatgaaaag
    25921 gctggaaatg caacatctcc gtgttcagct gattttggat tttctagtaa ttgttcgact
    25981 tgttccagtg ttaaatcatc tttgacagcg tcatagacag cttttgcgac aagatcttta
    26041 ttattcatct ttattcctcc taaagtgctc ccttgattgt taaacaaaaa aaatctctag
    26101 cgacggcata cttagtatgc catcactaga gacgagattt cccgcggtac cactccaatt
    26161 gtcctaaaaa aggaccagct ccatctgata tcggtagacg accgtctgtt taaaacagaa
    26221 ttctccaagt gcgcttcgtt gtatcttctt cattcggctt ccaccacttc cgaattcgct
    26281 agccaagaat gatacaagta ctctcttgtt caacaaatct tagtcttatt aaactttctt
    26341 gaattatatc agaaaaaaaa acgaaaggct agtcgtttct gataaaaaaa caatccagat
    26401 ggcctaaagg agaagaaata ccacctggat agctataaat catttcgtaa atacgaaaga
    26461 cttaacaaat taatcagcta cgataatcgt tccttcttca gaagctaaaa gattctcgat
    26521 attttctaaa gaagtgatga ttgccttagc gtttggtttt gcttcaacaa atgcaatagc
    26581 tgcctctact ttcggaagca tgcttcctgg agcaaattgc ttttcatcaa tgtattgttt
    26641 catttctgaa acagtaacag tttctaattt cttttgatct ggtttttggt aattgacata
    26701 cacattgtct actcctgtca atacgatcag caaatccgca tcgatgattt cggctaattt
    26761 ttctgaagca aaatctttat cgattacggc ttctcgtcct tctaatccag tagctgtttc
    26821 tacaacagga atcccaccgc caccaacaga gactgtgata actccttgat cgaccagcgt
    26881 ttcgatgaca cgtgcttctt taatcgagat gggtttaggg gaagcgacta cttttctcca
    26941 tccacgtcca gcatcttctt tgaatgtcac agtggagtca gcattcattt gttctttggc
    27001 ttcttcttcc gtgtagaatg gaccgactgg tttacttgga tttttgaatg aaggatcatt
    27061 ttcatccaca ataacttgag tgactaaaga aaccacatct ttatcgatcc ctttttcctt
    27121 cagtacttcg cccatggcat tttgtaacca gtaaccgata gatccttctg tcattgcaac
    27181 acatgtatct aaaggcattg ccggtgtttt ctcagaatca gcagcttgct gctgaatcaa
    27241 taggttaccg acttgcggac catttccgtg ggagatgatc aattcatctc cttgttcgat
    27301 aaatttcact aaatactttg ctgtttccat taacgcttct tgttgggctt ttgcacttgc
    27361 atccgtagac aagattgcat taccccctaa agcgacaact acttttcgtt tggccatttc
    27421 attcgtcctt tcttcagttc taccgaattt tctagataac aaaacgagcc agcgttttgt
    27481 gattttccct caatgctgac tcgttttttc gtgagatgaa tgtggttatg cttaaacgcg
    27541 agggataaac aagttaccta atgttgctgc catgatcgct ttgattgagt gcatacgatt
    27601 ttctgcttgg tcaaattgac gagcatattt gctgcggaag acttcatctg ttacttccat
    27661 ttcagtgatg ccaaagcgtt ctttcatttg ttcaccgtaa acagtgtttg tatcatggaa
    27721 agctggcaag cagtgcaaga agatcaaacg atctgtgttg tgtgtttttt ctaccatttc
    27781 catattgatt tggtaaggtt tcaacaattt gatacgttct tcgaatttat cttcttcacc
    27841 cattgatacc catacatcag aataaaggac gtctgctccg tctacacctt tatcaacatc
    27901 gtcggtaatc atcaattgtg caccggattt ttcagcaaag ccttctgcca ttttgacgat
    27961 ttcttcttca ggttgtaatt cttttggtgc aacgatacgc atattcgctc ctaaaattgc
    28021 acctgtgact aataaagagt tagccatatt gtttcgacca tctccgcagt aagcaacagt
    28081 gataccttct actgttccaa agttttcttg gatagttaaa aagtcagcga tcatttgtgt
    28141 tggatgccat tcatcagtca agccattcca aactggaaca ccagagaatt ctgctagttc
    28201 ctctaccatt ttttgactga atccacggaa ttcgattcca tcaaacatac gacctagtac
    28261 tttagctgta tcttctgtgg attctttttt acctaattgg atatcatttg cacctagata
    28321 ttcaggatgt gcacctagat cgatcgctgc agtagtaaat gcagaacgag tacgtgtaga
    28381 tgttttttca aataaaagcg caatattttt tccttctaga tagtgatgtg ggataccacg
    28441 ttttttcaag tctttcaagt gttctgaaaa atcgatcaaa tattgtaatt cttcttttgt
    28501 aaagtctttt tctgctaata agcttcttcc ttggaatact gattctttca tattaaacat
    28561 tctcctttag attgttttta tttaagatta aagaggaaat cccgacggca gctgacagta
    28621 agaccgccgt caggaaacaa attacttcaa gtcttcacgg acaagtggtt ggctcatgca
    28681 gcgtggacca ccacggcctc gtgacaattc acttgaattg atttctaaca ctttgatgcc
    28741 atagctacga agcaattcgt tcgatacata atttcggtta taagtgacta caacacctgg
    28801 agcgattgcc aatgtgtttg atccatcatt ccattgttct cgaggtgcaa cgatctcatc
    28861 tccattacca gttgggatca gtacaagatc atctaatcca agagcagctt tcatcgtttc
    28921 gtgtaagtta tctgaatgag taatcttgat atcatcacca tctggttcga ttgtaaagac
    28981 atccatctta ccgtctttac tttgaattgc tggatggata gtgaatttat cgtgatctac
    29041 cattgtaaat acggtatcca aatgcatcat tgcacggttg tttggaattt tgattgctaa
    29101 cactttttca aatcctgaat tttttgcgaa taggtggcgt gctaattttt ctaaagcttt
    29161 cgcattagtt cgttgagaaa tcccaacagc tactacttta tcgcttaaga tcaattcgtc
    29221 tccgccttcg atatgatctg gattttcacg atccaaccat acttcgattc ctttatttgc
    29281 aaatcttgga tgatgtttca aaatatattc tgtaaacatc gattcacgac gacgtgcttc
    29341 aaaagtcatc ttattgactg tcatcccatt tcccattgat gcggaagggt cgcgtgtaaa
    29401 gtacaagttt ggcattgggt ccatgtaaaa tggatagtca tcgtctgctg acagatcgta
    29461 tagactgcta gaacgtactt caatatcttt tgtacgaacc ccagccatga ttttatctac
    29521 catttcttgc gtttccattg atagaaggaa ctcatgaaga ccctctctta cagcattcga
    29581 tgcaatatga gattcatcca acattttatt caagaattgt tctttgacat tgcctgcgtc
    29641 aatcgcttct gccgctaatt tttccaagta aagagtctcg acgccttcat tttgcaatgt
    29701 ctttgcaaag ttgtcatgtt cttcttgagc aatcggaaga tatggaatat catcgaacaa
    29761 cagacgatcc ataatatctg gtgtcaaatt ttccacttct tgtccaggcc ttttcagtaa
    29821 aactgtcttt aatttcccta tttcagagaa aacgtgaata ggtttatcca tgttgtgttg
    29881 cctccttctt tttgattaca tgtataggat atcctccttt tgtaatcact ttcataattt
    29941 tgtctc
//
LOCUS       DAYYGS010000029        29136 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000029 DAYYGS010000000
VERSION     DAYYGS010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29136
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.029"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1029)
                     /locus_tag="ACHA44_001192"
     CDS             complement(<1..1029)
                     /locus_tag="ACHA44_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8904497.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="ACHA44_001193"
     CDS             complement(1111..1800)
                     /locus_tag="ACHA44_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8904498.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="ACHA44_001194"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="ACHA44_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFM8904499.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /gene="rplJ"
                     /locus_tag="ACHA44_001195"
     CDS             complement(2327..2830)
                     /gene="rplJ"
                     /locus_tag="ACHA44_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFM8904500.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3034..3161)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="ACHA44_001196"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="ACHA44_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFM8904501.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="ACHA44_001197"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="ACHA44_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFM8904502.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4798..5466
                     /gene="sdaAB"
                     /locus_tag="ACHA44_001198"
     CDS             4798..5466
                     /gene="sdaAB"
                     /locus_tag="ACHA44_001198"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HFM8904503.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5479..6351
                     /gene="sdaAA"
                     /locus_tag="ACHA44_001199"
     CDS             5479..6351
                     /gene="sdaAA"
                     /locus_tag="ACHA44_001199"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HFM8904504.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6442..7542)
                     /locus_tag="ACHA44_001200"
                     /pseudo
     CDS             complement(6442..7542)
                     /locus_tag="ACHA44_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324221.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            complement(7634..8521)
                     /locus_tag="ACHA44_001201"
     CDS             complement(7634..8521)
                     /locus_tag="ACHA44_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8904505.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="ACHA44_001202"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="ACHA44_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFM8904506.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="ACHA44_001203"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="ACHA44_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFM8904507.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="ACHA44_001204"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="ACHA44_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8904508.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /gene="cbpA"
                     /locus_tag="ACHA44_001205"
     CDS             9752..10396
                     /gene="cbpA"
                     /locus_tag="ACHA44_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-AMP binding protein CbpA"
                     /protein_id="HFM8904509.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="ACHA44_001206"
     CDS             complement(10452..10955)
                     /locus_tag="ACHA44_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8904510.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11552..12517
                     /locus_tag="ACHA44_001207"
     CDS             11552..12517
                     /locus_tag="ACHA44_001207"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8904511.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLILVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12616..13788)
                     /locus_tag="ACHA44_001208"
     CDS             complement(12616..13788)
                     /locus_tag="ACHA44_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904512.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13775..14920)
                     /locus_tag="ACHA44_001209"
     CDS             complement(13775..14920)
                     /locus_tag="ACHA44_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904513.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14934..15866)
                     /locus_tag="ACHA44_001210"
     CDS             complement(14934..15866)
                     /locus_tag="ACHA44_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904514.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15983..17062
                     /locus_tag="ACHA44_001211"
     CDS             15983..17062
                     /locus_tag="ACHA44_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8904515.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17059..17677
                     /locus_tag="ACHA44_001212"
                     /pseudo
     CDS             17059..17677
                     /locus_tag="ACHA44_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(17787..18014)
                     /locus_tag="ACHA44_001213"
     CDS             complement(17787..18014)
                     /locus_tag="ACHA44_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8904516.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18117..20705)
                     /locus_tag="ACHA44_001214"
     CDS             complement(18117..20705)
                     /locus_tag="ACHA44_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HFM8904517.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20725..21384)
                     /locus_tag="ACHA44_001215"
     CDS             complement(20725..21384)
                     /locus_tag="ACHA44_001215"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8904518.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLLNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21433..21936)
                     /gene="trmL"
                     /locus_tag="ACHA44_001216"
     CDS             complement(21433..21936)
                     /gene="trmL"
                     /locus_tag="ACHA44_001216"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFM8904519.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22157..23023)
                     /locus_tag="ACHA44_001217"
     CDS             complement(22157..23023)
                     /locus_tag="ACHA44_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HFM8904520.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23132..23773
                     /locus_tag="ACHA44_001218"
     CDS             23132..23773
                     /locus_tag="ACHA44_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFM8904521.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23835..25196)
                     /gene="mgtE"
                     /locus_tag="ACHA44_001219"
     CDS             complement(23835..25196)
                     /gene="mgtE"
                     /locus_tag="ACHA44_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFM8904522.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25214..26113)
                     /locus_tag="ACHA44_001220"
     CDS             complement(25214..26113)
                     /locus_tag="ACHA44_001220"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFM8904523.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26115..26912)
                     /locus_tag="ACHA44_001221"
     CDS             complement(26115..26912)
                     /locus_tag="ACHA44_001221"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HFM8904524.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26890..27594)
                     /locus_tag="ACHA44_001222"
     CDS             complement(26890..27594)
                     /locus_tag="ACHA44_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HFM8904525.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27725..28300
                     /locus_tag="ACHA44_001223"
     CDS             27725..28300
                     /locus_tag="ACHA44_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFM8904526.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28418..29095
                     /locus_tag="ACHA44_001224"
     CDS             28418..29095
                     /locus_tag="ACHA44_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP adapter SpxH family protein"
                     /protein_id="HFM8904527.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8573 a         6172 c         5239 g         9152 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tacatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
     4621 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
     4681 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
     4741 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
     4801 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
     4861 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
     4921 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
     4981 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
     5041 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
     5101 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
     5161 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
     5221 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
     5281 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
     5341 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
     5401 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
     5461 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
     5521 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
     5581 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
     5641 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
     5701 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
     5761 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
     5821 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
     5881 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
     5941 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
     6001 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
     6061 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
     6121 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
     6181 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
     6241 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
     6301 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
     6361 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
     6421 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
     6481 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
     6541 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
     6601 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
     6661 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
     6721 cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac catctgcccc
     6781 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
     6841 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
     6901 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
     6961 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
     7021 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
     7081 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
     7141 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
     7201 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
     7261 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
     7321 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
     7381 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
     7441 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
     7501 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
     7561 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
     7621 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
     7681 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
     7741 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
     7801 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
     7861 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
     7921 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
     7981 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
     8041 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
     8101 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
     8161 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
     8221 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
     8281 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    11521 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    11581 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    11641 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    11701 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    11761 cgatactttc ccccttttga tcctcgtagg aggtgacggc accttacatc aggtacttga
    11821 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
    11881 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    11941 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    12001 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    12061 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    12121 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    12181 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    12241 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    12301 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    12361 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    12421 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    12481 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    12541 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    12601 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    12661 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    12721 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    12781 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    12841 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    12901 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    12961 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    13021 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    13081 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    13141 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
    13201 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    13261 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    13321 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    13381 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    13441 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    13501 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    13561 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    13621 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    13681 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    13741 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    13801 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    13861 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    13921 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    13981 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    14041 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    14101 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    14161 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    14221 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    14281 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    14341 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    14401 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    14461 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    14521 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    14581 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    14641 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    14701 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    14761 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
    14821 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    14881 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    14941 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    15001 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    15061 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    15121 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
    15181 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    15241 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    15301 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    15361 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    15421 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    15481 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    15541 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    15601 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    15661 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    15721 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
    15781 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    15841 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    15901 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    15961 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    16021 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    16081 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    16141 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    16201 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    16261 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    16321 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    16381 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    16441 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    16501 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    16561 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    16621 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    16681 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    16741 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    16801 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    16861 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    16921 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    16981 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    17041 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
    17101 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
    17161 tgtgccaagg atgcgcgaaa cccatcgtcc cgatgttttg ctgacggaca tccgcatgcc
    17221 taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca aagccatcct
    17281 tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt ccatcggtgt
    17341 caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca aatccgctta
    17401 taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa tgatgaagaa
    17461 taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc ttgcaatcat
    17521 caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt acttgagtga
    17581 cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac gggatcgcac
    17641 gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca caaaaaaacc
    17701 tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca attgtcccgc
    17761 aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg attcgttgga
    17821 ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc tagttttgat
    17881 tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc tttttccatc
    17941 tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa tttatcttcg
    18001 gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata tcacactgaa
    18061 aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct ttaaaatcaa
    18121 gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat catcggatct
    18181 actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac agcaggttct
    18241 acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc cactgtttcc
    18301 attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag cttttctttt
    18361 acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc gaccaaagaa
    18421 tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt atccatgatc
    18481 cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt gacataggct
    18541 tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt gtagagcaaa
    18601 ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat cttgaattca
    18661 ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt ccattcgttt
    18721 cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac tttatcttca
    18781 gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa tacattcaat
    18841 tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt gtcgttccaa
    18901 tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat tttatttaac
    18961 gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc ttgtgcacgg
    19021 aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat ttgataagca
    19081 tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg cagacgtcct
    19141 tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg ataaatctga
    19201 ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac ttgaccagga
    19261 ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt cgtcggaatc
    19321 gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc aacaatcaaa
    19381 agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc attcagcccc
    19441 agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg tttagctgct
    19501 cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt tgaaggatcc
    19561 aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac agtagttttc
    19621 cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc agcgatagct
    19681 tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg aatattcttc
    19741 tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg ctggatcgaa
    19801 ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt ctcttcttgt
    19861 tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc aggctggatt
    19921 tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt ctccgccggt
    19981 acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat agctgctcga
    20041 attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc tgcacgtttg
    20101 aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat gatatccaaa
    20161 gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa cccgtaacga
    20221 ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc agcaagcatt
    20281 tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc agcaatgatt
    20341 ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt cttcccgatt
    20401 cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt tggcttttct
    20461 tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac taattctcca
    20521 taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac tacaatctct
    20581 ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt gtaaaaatta
    20641 ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat ttcttctggc
    20701 tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt cagttcctaa
    20761 aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag gctcttcagt
    20821 ctctaaaatc gtcaaactac tatttaacaa tccacccatt tcacgcaact cacttagctc
    20881 ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta cgaataaaag
    20941 tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac gtgtgattgc
    21001 cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat gattacgcaa
    21061 attcggcaga ttatccggat atttctggta catttcatcg atcgactgac cttctaaagt
    21121 acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac gagcaagctc
    21181 tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat aaattgcttt
    21241 aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac tttgtggcaa
    21301 aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt gtgtcttccc
    21361 atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt attttactct
    21421 ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt ctaaattagg
    21481 gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg ataaattcaa
    21541 tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt tttcacgcat
    21601 aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct cttctccatc
    21661 agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat gtagtttacg
    21721 attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat ttacatcatt
    21781 ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac ctagtggctc
    21841 gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc cagtgtttgc
    21901 agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc tcatatttat
    21961 gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat caactcggtg
    22021 tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta tcagaacagg
    22081 aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct caactttttt
    22141 ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt ccttttaata
    22201 attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct ttgattcgct
    22261 cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt tcctctggta
    22321 tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca gcaaacttgg
    22381 ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa aaagcctgac
    22441 gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca tcttctgaga
    22501 gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc gcgttgaatg
    22561 tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct tcggcagatt
    22621 gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca ttttgaatct
    22681 ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca tctaatagcg
    22741 agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac atgccagatt
    22801 ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta accgcatggg
    22861 ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt ttgcatatca
    22921 agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac agctgatgat
    22981 tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc cctcatttct
    23041 gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat acaatgccta
    23101 gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg ctgaaaacta
    23161 cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt gtgacaactt
    23221 agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa gttatgctgg
    23281 ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact tcgtctataa
    23341 cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat tagggacgga
    23401 tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg ccttagaact
    23461 attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg acagtattcc
    23521 tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg accgtatctt
    23581 gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc ttaaagaatt
    23641 gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca ctgcagagaa
    23701 tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca aaatcgtgga
    23761 tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg cttattgcaa
    23821 atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt gaaataaatc
    23881 agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac tgctggatca
    23941 aatcctagac gatccattag catcgggatt aaactacctg caagattcgc cactgtgatt
    24001 gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt ccaaaaacct
    24061 acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt caagacttca
    24121 gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag tcgacgaaca
    24181 gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag cgaaatgaag
    24241 actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa agaagcggtc
    24301 gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc tgcctttact
    24361 ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc gctagcagct
    24421 tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa atgatcatca
    24481 taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc cacatcttct
    24541 tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga aatcattgcg
    24601 tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg accttcttga
    24661 ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt cagcacgtac
    24721 atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt catgatcgcg
    24781 ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc gtctgccgac
    24841 atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa gtcaacagca
    24901 ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat ttcatctagg
    24961 tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc taattcttta
    25021 ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg gtagaattgc
    25081 ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat attattttcc
    25141 cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc atccaaacca
    25201 atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct gcggctaaag
    25261 gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga tcataacaat
    25321 gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca tcaccgagca
    25381 gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct gtatgcagct
    25441 ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca gtctgtgccg
    25501 aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg gcgatcggca
    25561 aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag gcctgatatt
    25621 ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca aatccatgct
    25681 ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca tggattactt
    25741 ggttttcgta accttgtttt ttataataag cctttacacg attagccatt gtcccatttg
    25801 gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc agaacgtgtt
    25861 catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca tgttctcctt
    25921 cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag acattttcta
    25981 cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac agaccctttg
    26041 agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc tcataatccc
    26101 atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg ccaaaaatgc
    26161 atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata aattgagcgg
    26221 atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc tgtattttcc
    26281 aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa agtacgaaac
    26341 actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact aggatggatc
    26401 acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga aatcgtcaat
    26461 ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc taccatcgta
    26521 cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc cggtgtttca
    26581 tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc ccggttcgtt
    26641 cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta aaaccctaaa
    26701 tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta gtgatggaac
    26761 gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc atgatcatct
    26821 cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc tatagtcttt
    26881 gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt cgctttcttt
    26941 cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg agaataaccg
    27001 ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact gataggcagc
    27061 ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt gatatttata
    27121 attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa tctggatttc
    27181 cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca ccagatgata
    27241 agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga gaactttcat
    27301 gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa actgacacat
    27361 gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg gaatatgccg
    27421 taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga caaattcaat
    27481 cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt tcactttcaa
    27541 ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt ccatagctga
    27601 tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt tttttcgtac
    27661 tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa aaggagtcga
    27721 tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc aagagtacga
    27781 gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga ctaatattta
    27841 tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga tccgtttcgt
    27901 tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc tcgaaacaac
    27961 ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc tgcctcgttc
    28021 aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat taatccaaac
    28081 cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt tagctattga
    28141 tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag gggatgcatc
    28201 tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt atcgtcctgc
    28261 taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca ttttcacaag
    28321 gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa agaatctagt
    28381 cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct atttatttgt
    28441 taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat ttatcgaaga
    28501 agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt tggtcaattt
    28561 gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc ttgaggaacg
    28621 taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg cgcagtttca
    28681 aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg gctgtaatga
    28741 cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg gcgatttgaa
    28801 tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt ctgatcaaaa
    28861 aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca attatgcctg
    28921 cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt ggagaatcac
    28981 agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac atttacatac
    29041 aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta attaaaaaag
    29101 gctctttgtc aaatgatgtt ggtaaagaga aatctt
//
LOCUS       DAYYGS010000030        28078 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000030 DAYYGS010000000
VERSION     DAYYGS010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.030"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(193..1092)
                     /locus_tag="ACHA44_001225"
     CDS             complement(193..1092)
                     /locus_tag="ACHA44_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8904528.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1102..1458)
                     /locus_tag="ACHA44_001226"
     CDS             complement(1102..1458)
                     /locus_tag="ACHA44_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8904529.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1813..2109
                     /locus_tag="ACHA44_001227"
     CDS             1813..2109
                     /locus_tag="ACHA44_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HFM8904530.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2753..3706)
                     /locus_tag="ACHA44_001228"
     CDS             complement(2753..3706)
                     /locus_tag="ACHA44_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8904531.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3729..4484)
                     /locus_tag="ACHA44_001229"
     CDS             complement(3729..4484)
                     /locus_tag="ACHA44_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904532.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4481..5440)
                     /locus_tag="ACHA44_001230"
     CDS             complement(4481..5440)
                     /locus_tag="ACHA44_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8904533.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5433..6383)
                     /locus_tag="ACHA44_001231"
     CDS             complement(5433..6383)
                     /locus_tag="ACHA44_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8904534.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6533..6985
                     /locus_tag="ACHA44_001232"
     CDS             6533..6985
                     /locus_tag="ACHA44_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HFM8904535.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7074..9341)
                     /gene="gshAB"
                     /locus_tag="ACHA44_001233"
     CDS             complement(7074..9341)
                     /gene="gshAB"
                     /locus_tag="ACHA44_001233"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HFM8904536.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9528..10526)
                     /gene="ftsY"
                     /locus_tag="ACHA44_001234"
     CDS             complement(9528..10526)
                     /gene="ftsY"
                     /locus_tag="ACHA44_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFM8904537.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10537..11346)
                     /locus_tag="ACHA44_001235"
     CDS             complement(10537..11346)
                     /locus_tag="ACHA44_001235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8904538.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11355..14936)
                     /gene="smc"
                     /locus_tag="ACHA44_001236"
     CDS             complement(11355..14936)
                     /gene="smc"
                     /locus_tag="ACHA44_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFM8904539.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14956..15642)
                     /gene="rnc"
                     /locus_tag="ACHA44_001237"
     CDS             complement(14956..15642)
                     /gene="rnc"
                     /locus_tag="ACHA44_001237"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFM8904540.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15990..17771)
                     /locus_tag="ACHA44_001238"
     CDS             complement(15990..17771)
                     /locus_tag="ACHA44_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8904541.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17797..18711)
                     /locus_tag="ACHA44_001239"
     CDS             complement(17797..18711)
                     /locus_tag="ACHA44_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904542.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18738..19700)
                     /gene="opp4B"
                     /locus_tag="ACHA44_001240"
     CDS             complement(18738..19700)
                     /gene="opp4B"
                     /locus_tag="ACHA44_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease"
                     /protein_id="HFM8904543.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19700..20644)
                     /locus_tag="ACHA44_001241"
     CDS             complement(19700..20644)
                     /locus_tag="ACHA44_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904544.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20645..21649)
                     /locus_tag="ACHA44_001242"
     CDS             complement(20645..21649)
                     /locus_tag="ACHA44_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904545.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21917..22159)
                     /gene="acpP"
                     /locus_tag="ACHA44_001243"
     CDS             complement(21917..22159)
                     /gene="acpP"
                     /locus_tag="ACHA44_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFM8904546.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22281..23282)
                     /gene="plsX"
                     /locus_tag="ACHA44_001244"
     CDS             complement(22281..23282)
                     /gene="plsX"
                     /locus_tag="ACHA44_001244"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFM8904547.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23331..25367)
                     /gene="recG"
                     /locus_tag="ACHA44_001245"
     CDS             complement(23331..25367)
                     /gene="recG"
                     /locus_tag="ACHA44_001245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFM8904548.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEGSEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25484..27166)
                     /locus_tag="ACHA44_001246"
     CDS             complement(25484..27166)
                     /locus_tag="ACHA44_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HFM8904549.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27218..27580)
                     /locus_tag="ACHA44_001247"
     CDS             complement(27218..27580)
                     /locus_tag="ACHA44_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8904550.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27850..28038
                     /gene="rpmB"
                     /locus_tag="ACHA44_001248"
     CDS             27850..28038
                     /gene="rpmB"
                     /locus_tag="ACHA44_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFM8904551.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7765 a         6080 c         4950 g         9283 t
ORIGIN      
        1 tgtgattatc gttcaaaaaa agaatacacg cttgcatcta gtactctgcg agttacaaat
       61 catccgatca aataaacatt cgctgattat tttccgcaaa aaaagcagca tatccacgat
      121 aaatggatct actgctctta cttatcctta tgttttacaa ggtattttct tttacacatt
      181 actaatgctg tctcaatacg gaacgattgt catcgcttgt tcaaattcct cttgcaaatg
      241 ttgtgtttga taggcctgaa aagctgggtg atgggcacgc aagaggacac gtaatttcat
      301 aggatcatag ctgtctagca actgttcttt caagacaaaa tctcgggtca gctgccattc
      361 ataataataa ccaaaaggtc tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa
      421 gcgacgatgc gtatggccaa aaactacttg tttgatattt gaataacggt caagtaattc
      481 tccgaatgat tctgatccta aaaatgcatt taatttgttc caacgctcat aaggagcaga
      541 ttgatacatg ataaatgccc gtttaggaac gaaatgagtg gataaaatca cctcaagttt
      601 ttttgcttcc agctggtcta gcaaattttt tgcagctgca agcatttctg cattgacctc
      661 gagatctgtt ttcccacgct ggatgaaccg atcataccaa tacaaacgct tcattcgtac
      721 gattgcctcg taatctttca attcggaaaa ctgatagtcg taccagccat tcatccctaa
      781 aagaacggtc tgctcattta aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa
      841 atggtccatc atctgttcat tgggcacatc tgccaactcg tgattgccga aatgaaatgt
      901 cgtagataat cctttattga cgaaaaattc gacaacagct aggcaacggt ctactttatt
      961 agccgtatct ccggctagat gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag
     1021 ctgcatcagc tctgattcgc cgaatttatt gatgtccaca tgtaggtcgc tgatgatcgc
     1081 caattttccc attttatccc tctagtcttt tcgaacaact tgcaagttgc cttttctcaa
     1141 gccgcttggt ccttttaatt gtaaactatt ataagttggt tctaaagcaa gtgtggatgg
     1201 atcatccatg taaagtattg cactgtcttt gattttgatt ggtccaactg gtgtatcagc
     1261 cactacagaa taaagactta gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa
     1321 tgccgtatcc aattggcaac tgacttggct ttctgcaaat ggaccgtcac cgttttcgat
     1381 atctaataaa agttgatcgt ctgcttctaa tttattcttc aatacttctt gtgcttcttt
     1441 tgtcacttct aatttcatct gtatcacctc atgtcataga ataatcactt tattcatcag
     1501 atgcaactaa tttgtctggt tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt
     1561 tattatgatt tctcttatca caaaagagac aactttccct tttcttcgtt cagtttcttg
     1621 aacggaaaaa gacccaaaaa gcaacctgtt atttgtttaa ccttattcct ttcttaaata
     1681 acaaaaaata ctataaaaaa taaaaaacct ttaacaacta ttggaaaaag aattactttt
     1741 tgttcacgga ttattaacaa ctcttcggta gagtaaactt atcaaataaa agacttcttt
     1801 ggaggaatca ctatgaagga atatataaaa aacatctatt ttatcgaaga aacacaaaat
     1861 atcgagggtt cttatattga agtaaaaaca ctctttgtca atgaagataa aaccaaagca
     1921 ttggatattt ataaaaaatt agcttcaaaa aaaacgaata gttttggatt gatattaagc
     1981 gagtataaaa tcaaagcaga agagagttat ttctatcaat tactaaaacg gtggagtaag
     2041 cttcccgccg atttttatcg aaaaatgcag atcatcaact atcaaccgct tgctgagaca
     2101 catgcctaaa tagaggttgt gaaatcagtg ctttgagcta ataccgaagg tcagctgatt
     2161 gaacattgtt tagaagagat tgtgacaact gcgatttgct ttgagtaata agaacgctgg
     2221 acagaaaata gcttctcata ttttctagtc tagtgggctt attgccgaga agcagcagat
     2281 ggaacatcgt ttagaagaga ttgtgacaac tgcgatttgt tttgagtaat aagaacgctg
     2341 gacagaaaat agcttctcat attttctagt ctagtggact tattgccgag aagcagcaga
     2401 tggaacatcg tttaaagaag agatcggtac aaaataaaaa tatctaaatt taagttttat
     2461 ctaagtttcg ctttgtacct ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa
     2521 tggatcacac tattttttgc cactccccat tctagaatca acccgtatga tgtagataat
     2581 gaattcacgt tgaaaaaaga tttctcaaat aaataaaaac catccgtaaa atcatcaata
     2641 cgattttacg gatggttttt gataacttct ttgaggatag gctcttgcta tttaagaatg
     2701 taagaaacat tattgttaca tagtatgaga gtttaatcct ctgttattta tgctatttaa
     2761 atgcttgatt cacatcttcg atcatcagtt tcattgattc taaaccgccc ccgcttaaat
     2821 accagacatc tggttcaagg gagatgatct gctgattttt accagcattc gtttgtgcta
     2881 ccagttcatt ggcagaaata tcatctttgc tgtcgtctcc accaatagct tttgttctgt
     2941 ctactacaaa taagatatcc ggattttttt ccagcacata ttcataagaa acactttgac
     3001 cgtgagtgga tgcttcgatt tgatcatctg cttgttcaaa gccaaataaa tcatggatga
     3061 aaccgaatct agaaccagat ccgtaggctg ataattgccc ttcattcaca agtgtgacta
     3121 gtgctttttt gtcagtagct tctgctttct ttttcgtctg atcgattgca ctagataatt
     3181 cctctaattg agttttagct tcttcttgct taccaaagat ctcccctaaa gcagtgacat
     3241 tttgctggaa agattcccat ggatttttcg catctaaaga aaggaaaatg gtcggagcaa
     3301 tttgacttag atctttttga aaatctcttt gtcggccaga aatgatgatt agatctggct
     3361 gaagctgatt aattttttct aagtctggtt cttttattcc acctgctgat tctacttttt
     3421 tataggatga aagatactca ggaaggctgc ttgtagctgc tccaactacc ttatctccaa
     3481 caccaagagc atccatcgta tctaaagaac cattgtcgaa tacgacgact ttttctggat
     3541 tatttggcac ctcgatttgg tctccattac tatctgttat cgtcaatgta gtttcagttt
     3601 gtgtgctgct tttttttgtg tctgcctgac tggtgttgtt gctatttcca catgctgcta
     3661 aaccaaatga tgctagaatc aagataccta ctactaattt tttcatctct tctctcctaa
     3721 tgttagtttt agaaataaag acagaatctg cggccttcaa tctcacaaat ccgtatgtcc
     3781 atttcgtata attcatccaa tacatctttt tttatcattt cttgggtcgt tccttgcgta
     3841 aataactgtc catctttcat tgcaacaatt tcgtctgcat aacttgcagc aaagttgata
     3901 tcatgaagaa caataacaat tgttttcccg atttcttgta ctaaattttt taaaatctgc
     3961 atgatttgaa ccgcaaaatt catatctaga ttgttcaaag gttcatccag taaaatataa
     4021 tccgtgtctt gcgctagcac cattgcaata tacgcacgtt ggagttgacc gcctgaaaga
     4081 gtgtcaatcg attgatccgc tatttcttct aacccaaggt gttccagtga ttcctgaacg
     4141 atttcctcgt cttttgtcgt caattttcct ttcgtataag gaaatcgtcc aaattctact
     4201 aattctttca cggtgatttg gacatttatt tcattcgatt gtcgtaggat cgataatttt
     4261 ttggctaatt cattggattt ccaagccttt acttcgttat ggtctaaata gatttcacct
     4321 gtatcttttg gtatcaaacg actgatcatc gacaacaccg ttgactttcc agcaccattt
     4381 ggaccgataa aagcagtcaa cttcttttca ggaatcgtta actgtacatc tttaaccact
     4441 gattttccat ggtacagttt attgaccttt tgtaaatcaa tcataggata ctcttccttt
     4501 cgcgaatcaa ttttgtgaca aaataaattc ctccgacaaa ttcaacgacg acactcactg
     4561 ttgtattcca gccgaaaatc tgctctactg cgagctgtcc tcctgtcaga aacagcatag
     4621 ctaccagaaa gcttccgata aatagctgac catgacgata agtagaaaag acacgataac
     4681 tgatatttgc gacaataaac cctaaaaaag taactggacc cacaagagcc gttgatgtcc
     4741 ctgtcaaaag actgacacaa cataaaatca aaaattcaaa acgatctgtg tctatcccga
     4801 ggtttactgc ttgttcattt ccgagatgga aaacgtctag cacatgtcga tatcgaaata
     4861 acaggtacca gcagccgata atgatcaaag aagcaggcaa aagatactga ctctggatat
     4921 tgccaaagct tgcaaataat ctgccttgaa gcaaatcata ttcattcgga tccatgatga
     4981 cttgtaaaaa ggtactgaca ctaccaaaaa gcgtacctaa aatcatccct gacatcagta
     5041 ataaaaaaag attcgctctc atttttttca aaagaaagaa aaataacaat agactcagcc
     5101 cgaccatcag cccaacgttc aataagaaaa aaactgtacc tgtctgctga agcatcgtca
     5161 ctcctccgat aaagaagaaa aggaccgttt ggacaaggac atataacgac tcaagtccca
     5221 atactgaagg agtcaaaaac tggttatgcg taattgtctg aaagctgatt gtggcactgc
     5281 tgacagctag cgagacaagg ataaatgcaa aaattttctt actcctcaat gcacatgcaa
     5341 aatgccagtt tccatatgta tggatcgtca agtacattcc tgtaacaaat aaaacaagaa
     5401 caagcatacc aataaaaata gatggctttt ttttaagcat atcgtcgtcc ccttatcaga
     5461 agataaagga agatagcccc accgatgatt ccaacgatca cactgactgg tatttcataa
     5521 ggaaaaatca acacgcgagc gacaagatcg caaaatacta agaacaaact acctgctaaa
     5581 gcagttacgc caattgtttt gtgcacttga tctccatata ttctagagac aagattcgga
     5641 atgatcactc cgataaaagg aatgcttcct accatgatca acatgactgc actagccaat
     5701 gatactaagc caatgcctaa aaactgcatc ttctggtaat ctattcccag atttactgca
     5761 aacgattcgc ccagaccaat gatcgtaaat cggtaagcaa atagccaaac caagagcaac
     5821 aatggaacaa cagcatacaa taactcataa tttcctcgta tcacagtcgc aaaattccct
     5881 tgaagccatg aagtcatatt ttgtaccaac tggaattgat aagcaaaaaa agtagcaact
     5941 gaaccaatga tattccctaa catgactcca atcaacggaa gcatcaattg atttttctgg
     6001 aggaacaatc gcgtaaggga taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct
     6061 gtcaaggatc ggacaatcaa ggatgcacca ggtaggaaaa tcatagccac taaaatacct
     6121 aaacgcgcac tatccatcgt gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa
     6181 tgttgcatga tcaacccaca aatactactg gtcgctccca caaggatcaa acaaatcgtt
     6241 cgtggcaaac gagtcactag caagatttgt aagctttgcc catctaactg gagcagttct
     6301 gacaattgta cattcgatac tccgatgaac aatgaaatac tacttaaaat aactaataat
     6361 gctactagtc gtccgtagcg catccaatcc acctttctaa ttgagaatga ttttcattct
     6421 tattttaagt gaatcgttga acaaaagcaa ccgctttttc atatgtttta ggtattattg
     6481 ggaatatgct ataatcgtaa gaaagacaaa gagaagaagg agggtatttt ttatggcaca
     6541 agatgatgta aaaagccatt tggataaggg actttatggt acacctttag tgaaccctga
     6601 agaacagcat aaatatttag ggacattcag ggaacgctgc tatcttagta tgactgttgc
     6661 tgaaatgatc caaccaaaaa acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga
     6721 tgcttccata ctcttgaatg gggctctacc cctttctgtc caaaaccaat atatccagct
     6781 agcaacgaaa aacaatagcc gtttcactgt agtcaacgat tacgtcactg caaatcctga
     6841 ggaattcgga ttgctcttaa cagcaaaaga agcagtaaat gaacctgtga ttgatatcga
     6901 aaaaaaatac ccagaagaaa actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa
     6961 aagcttctgg gatcgtttat tttaaaacga aaaaacacat aaatttttac atgcaaaaaa
     7021 agcttttgat cagattgaca tcaaatagga caatctgtca aaagcttttt cttttaaaat
     7081 acttcaggat ataacaattt caatacattc atcgtcaatc ttctgccttt tcctgcaaaa
     7141 ggataaacat gcatatgcat ggctggattg aaatttgctt cgatgatccc atatgctttt
     7201 ttatcagttg taggatcgat ttctttatct ggtataatca aatcgatacc actaatctta
     7261 gctcctagtg cttctaccgc agaaacggcg atttttttgt aagcttcaga aaattcatca
     7321 gtcatatcga tcgaatcacc acctgtactg atatttgagt tctcccttag ataaacaatt
     7381 tgattggctt ggggaactga ttcgatcgtc aacccttgtt ctttcaacat caattgttct
     7441 aattcaccta attggatcaa ttctaaaggt gcacgatgat tcgttccacg caaagggtca
     7501 ctattttttt cttcaacaag ttctttcact gttcggatac tgtcaccaat aacattagca
     7561 gggactcgca acatgatggc ttggacttct ccgtcgatca caaagaaacg atattccgtt
     7621 cccggcataa attcttctac taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct
     7681 gcttgataat cttccagact agctccttcc ttgaaaatcg tgatacctaa cccgtaattg
     7741 gttgatttag gtttgatcac aaatgcctgc tcggcaaatc tcggataagc ttttaccgct
     7801 tcttccatgg atgaaaattc tgcgcctcct ggtacccgga acccagcttc ttttaagact
     7861 tttttcgtta ctgttttgtt ttccatgata agtgggacga tatagctgtc cttacttgtc
     7921 atgtttgcat ttttcacata ttctacatgg tcttggaatt gcagacgcaa aaattgatcc
     7981 gactcatcta atactttgac ttggacacct ttttggatgg catcgaacat aaatatctga
     8041 gtagaaagtt ccatttcgcg aaaaccggcc aactgatagg gacgttcatg gctttcttta
     8101 taatattgat gtcccaatgc cacagcaact tcttgctgac tcgatagctg tgcttcttga
     8161 tagagtttcg ctgctaacgt ttgttcagga tgatccattt gagtcaatgc ttcctctact
     8221 aaataaagag aatccaacca atccaattca accagcattt gcttcattcc ttcaagaatt
     8281 tctctacctt cttgatgaaa ttctgtttga tctgatggtt gttctagtgc tactttgtta
     8341 tttcgagtcg taccttctgc tacccattgg tcacagtctt ctttttcttc tgtccataac
     8401 atgtacatca ggaataattg taagaaccga acttgctctg gattgatacc caatcttgca
     8461 taaggattca aatcgatatt ccgcaattca atataacgaa cacctgttgt tgctaaatct
     8521 gcgactttct ttccgcctcg gaaacgaagc ggtgtataaa attctttctc ctccgaaagt
     8581 ttcccttctt cgatcattcg ctcgatatct gctaaatact gtttcataga agcataggaa
     8641 actttcacat ttggatgatt cgtatagccc agcgcactgt tgcgaatact gcgaacaggt
     8701 tcttgcggat gtgattcatc taaaaagtag ttcttctcac tcattggtga agcaccgaat
     8761 aaataagtga tcatccagcg atagcgcata aagtttctcg ctgttttcaa ataaagttct
     8821 gttttgaatt cggaaaaatc ttgaaattct tcctgatggc tgaacaatgt tcttagcaac
     8881 tcatcgccaa attcaaaatt gaaatgaata ccgcttacca tctgttttcg cttcccgtat
     8941 ttttttgcta aataacgtcg atacaagaca tcttcgaaat tttttaattt tgcaataatg
     9001 atctcttcgt ctttttccgg taaggcaggt ggcatactta atggccagat catctcttct
     9061 ttcggtatag aacgagcagt cacatcataa acagcagcga gatactgaaa taattcggga
     9121 atagaatcgg taacaggggt gatcatctct gtttgtgttt cgctaaaatc tgtttggata
     9181 taggggtgat aggatcgatc gccaaagatt gctggatgat cggtttttgc tagatttcct
     9241 gcaagatcaa cccgctgtcc ttcccgttct ataccaaatc gagcttggtc gataaaaggg
     9301 cgcgcattga catgcaataa taattgctta aaattcatca ttaagtgacc tccaattcat
     9361 atgaatctaa ttataatcaa aaagtctaag aaaaagaacg aaattgtttg agaaccgctt
     9421 acaaataagt ataaaaatga aggcatcaaa aacacagcta tgttaaaaac aatcattcaa
     9481 acaaggagct gcaacttttg tcacagctcc tgctttttag acatgggtta ttcttctttt
     9541 agtaatcctt tgaacaatcc aacgacaaaa tcatttggat caaacggctc caagtcatcg
     9601 attccttcac caagaccgac caatttcact ggtaaatgca attcattacg gatagcaagt
     9661 acgataccgc cctttgctgt accatctaat tttgtcaaaa caagaccggt cacatctgtt
     9721 gtttctttga attgttttgc ttgaaccatc gcgttttgac cagtggttgc atctaataca
     9781 agaagtgttt cttggggtgc ttctggtaat tcccgctgga ttactcgttt tattttttct
     9841 aattcgttca tcaagttcac tttattttgc agtcggcctg cagtatcaac tagtaaaaca
     9901 tctgcctgct ctgtctttgc tctttccaaa gcatcgaata cgacagctgc tggatcgcct
     9961 cctgcattgc cacgaacaac ttcaacacca gcgcgttcgc cccagacaac taactggtcg
    10021 attgctccag cgcggaaggt atctgctgct gccatcaata cttttttacc ttcgtttttg
    10081 aactgatggg caagtttacc aatacttgtt gttttcccta caccattaac accgacaaac
    10141 aaaataactg ttagtccgtc ttcttggata tttagttcat ttacttcatt gattccttcc
    10201 gcttcataga gatccacaag tttctcgatg atcgcatttt ggacttctgc cgattttttc
    10261 gcatttctga gtttcacttc ttgacgtaat gcttcggtga ttcgtatcgc tgtttcaaaa
    10321 cctacatctg caccgataag tgtttcttct aattcttcaa aaaagtcttc atcgactgtt
    10381 cgaaaattgg caaacaactc attcatccgt tcaccaaatg ttttacgcgt cttttctaaa
    10441 cccttgtcgt acttctcctg tacttcaact tcttctgttt tctcttcgcc agtaaaagca
    10501 cgcttgattt tatcaaaaaa tcccatttga ctccccctac tctaatacaa atttttggat
    10561 cactttggct acgccatttt cttcattgct ggctgtcacg aaatctgctg cttctttgac
    10621 gaaaggcaca gcattctcca tcgctacgcc tagtccagca tattggatca tcggtaaatc
    10681 attttcctca tcaccgatcg ccatgatttc ttcttgtttc aaaccaagat cttttgccaa
    10741 taaagaaata ccataagctt ttgtaacacc ttttggcata aattccaaaa gcatacttct
    10801 tgttttgatg atctcataca tttcatggaa ttctgctggt atttctttga ttttaggatc
    10861 taattcttct tggtcataag cgattactgc cttattaagc acaaattcat ctgtaatatc
    10921 gcttatatcc gctggttgga attgcagcag cttattcaac acattgtaca aggattgctt
    10981 ccttggggca ctaggcagct gtaaaaccac attgtcagac aaaacatcta gtggcagatc
    11041 caattgctga gccagtttgt ataagcgttg gatatctgat acttccatca ctttcttttc
    11101 catgatttca ccagtatcat ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc
    11161 accttcatct accagtccta attcttgcag ataatgcgcc atcgcagcta aggggcgtcc
    11221 tgtacataga acgatcttga ccccttgctg ttttgcttgt gctaatgctt gtttattctc
    11281 atctgaaatc agcttttgtt catttaataa tgtaccgtcc aaatcgattg ctactaattt
    11341 gatcattttc gttcctacac tttcgtttcg ttgattttcg cctcttcatt gatatcttcc
    11401 atccgtacag aaacgatttt tgatacaccg gattcctgca tcgtcactcc gtacagtaca
    11461 tctgctgcga ccatcgttcc tttgcggtgt gtgactacga taaattgtgt gtcatcttgg
    11521 aaatcgctca agtaacgacc aaaacgtttc acgttggctt catctaacgc tgcttctacc
    11581 tcatctaaga tacaaaacgg aacaggacag acacggataa tggaaaagag caacgcgatg
    11641 gctgtaagtg ctcgttctcc tccagaaagc aaacttagac tttgcagttt tttgccagga
    11701 ggttgtactt caatctcaat acctgttttc agcaaatcag ttggttccgt cagtactaat
    11761 tcagctttac cgccgccaaa catatttgga aagacaattt tgaattcttg acggatagct
    11821 tcaaatactt ctttgaatct tgtccgcact tcatcatcca tctcatccat cgtttcaaac
    11881 aattggtttt tcgctgctaa cagatcatct cgctgtgttg ctaagaatgt atgtctttca
    11941 ctgacttgtt cgtattgttc gattgaattt aagtttactg gtcccagttt ttcaatctgt
    12001 tcttttaagg atgaaacctt tgttctactg tcttcgatat cggttgtttc ttgatagtct
    12061 tgggaagctt tctcaaatgt cagctgatat tctgactgca gataggtcaa atgattatct
    12121 aaaatcattt cagctcggtc tttttggact tctaatttgg attgttccga cagtcgtgct
    12181 ttttgctcac gattacgctc tgccaagact gcttcaagct tatcgatctc cttttgcctt
    12241 ctatctcgct gctctttggc ttttgccaac tcggctttta atgtttctcg ctgagcagat
    12301 aattcgttaa tttgtttttc caaactttct tctgtgactt catgatcaga aaagtctgct
    12361 gtcaatgtcg ccaactgctt ttctatcgct tcttgacgtt cggttgcctc tgctttttgg
    12421 actcgggcac cgcggagctg gatctgcaga tgattaaact gctctttcaa gacagcaaga
    12481 tctgcttgtt cttgtgcttt ttgactttgt acttgtgctc ttctggcttc catctggtcg
    12541 ctttcttgac ttaacgattt gatttcttcg tcgatttttt gtcgttgcga ttcaagatct
    12601 gtttgtttgt ctttcaattc gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt
    12661 tcacgtgtct caaaagttga gatttgcttt tctttttcaa aacgttcaag ttcatttatg
    12721 atgttttgta gctgatttgt cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta
    12781 cgcaaaactt cttgttcttc ggctaaacga gctgtttctt gttgcagctc ctgaactttt
    12841 ttttcttgtg cttggagttg tttatctgct tcttcaaatt cactagtcaa ctgtttcaat
    12901 tcttgatttt gtacaaacaa actgccttgg tttcctcgtt tattcgctcc acctgtcatg
    12961 gatcctccgg cattcatcac atcaccttct aaggaaacca ctcgatattg atagcggatc
    13021 gtttgcgcga ttgcattggc actagttaag tctttggcta aaaggatcgt tcctagcagg
    13081 ttttgaacca cagtttggat ttgatcggga taactgactt gttcactggc aatccctata
    13141 aacccttcta cagctgctgc ttgtgtcaat atatgagccg gtaattgtct tggtttgatt
    13201 gtcgtcagtg gaaggaatgt tgctcttcct cctctttgct gtttcaaata cgtgatggct
    13261 tgccgagcat ctttttcatt ttcaacgatg acatgctggg ctgccccacc taaagctgct
    13321 tcgatagcta acgtgaaatc tgcaggaaca tcgatcagtt cagccactgc tccgacaata
    13381 cccgaaagct gctgtttatg ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag
    13441 ttttcttgga tttcttgaag gcttttctta cgagcacgta gctgctggac ttgattcatt
    13501 aattgataca ttttaggctg ttcttttgct aatgcttctt ggaacttttt cccgttttgt
    13561 tggatcattt ctaacttctc ttttgtttca gttaaagatg attttagctg cgcctgcttt
    13621 tgtgtcaatt cttctttttg taagatcaat cggtctactg atgcttctac ttccgattgc
    13681 ttggctaacg tctgcttgct ttttgctgtt tcttgtatat attgacgttc tagatacttc
    13741 agttcgtttc ctacagctgc ttgttcttgc atcaaatcaa catattgatc tcttaattct
    13801 gccaataatt ctttcgttga tttttggtat ttttctaatt cttgctgtgt ttttatgaga
    13861 tttgcttcag ctttttggat ctctgtttct ttttctgctg ctgctttcat caagctttct
    13921 tgaagctttt caaaatgttt aacctttttt tgtgcttctg ctaatgaagt ttgatattct
    13981 tgcgagcttt tttgtgtatg ctttgtccgc tcctgcaaaa catctttttg tccttctgtc
    14041 tgcttcagtt tttccgaaag atctaataat acttgctgat ttttttcgat cagacgatcg
    14101 gcttgcgcat tttctttacg ctgcttcgcc aaaatggatt cttgttcttg gatcgactca
    14161 gatagtttgc ctaattctag attgaatttc gcaagctgtg cttgtttgtt gtcccaatct
    14221 ttttttgctg tcttgatttc agcaaccatc aaagagacat cggtttgagt cagtgtttct
    14281 tttagccgta aaaactcctt tgctgcctca ctctgggcgg ccaaaggagt taactgttct
    14341 tccaattcgt ggatgatgtc ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc
    14401 tgttctgctt tctttttccg ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc
    14461 ccacgacggt cttccggttt actactgaaa atcgcttcta cttttccttg agaaatgatt
    14521 gagaacgatt cttttcctaa tccagaatct aagaacaatt cttggatatc ttttaatctg
    14581 cagctttgtt tattgatgaa aaactcgctt tccccagtcc gtcgataacg gcgggttaca
    14641 ctgatctcat tgtactccaa tggcagataa tgatcactat tgtctaaaat cactgtgact
    14701 tctgcaatat tcagctgctt acgggtatcg gaaccggcaa agatgatatc cggcattttt
    14761 ccgcctcgca gacttttagc agattgttct cctaataccc agcggatagc ctctgtaata
    14821 ttgctttttc cgctgccatt tggtccaacc actgctgtga cgctattttc aaaatcaatg
    14881 actgtcttgt ctgcaaatga tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct
    14941 ttccctcact tacttttaca gtgctgccaa tgcattttcc gccgcagctt gttctgctaa
    15001 ttttttcgat ttcccttgtc cagtgccaat caattgatca tctacataaa cttcgatcca
    15061 aaatactcgt tcatgagctg gaccttcttc attgatcaat cggtattcga ttgatacatc
    15121 acctgatttt tgcaagactt cttgtagttt tgttttatga tccatctcat gtgaaaaagc
    15181 acctgctttg actttaggaa aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc
    15241 ctggtcaaga tataacgcgc ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg
    15301 cgtacgtcct cctgaattct cttctccctt gcccaacaaa acatattgat caaagtgaca
    15361 ttctttggca aatttcgaca agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa
    15421 ttttccttct ggcaaatgag gatataaacg gaataaatac tgagaaacca tcagttctaa
    15481 aacggcatct cctaaaaatt caagtcgctc attatccgat aattgcagat tgcgatgctc
    15541 attcacatag gatgaatggg taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat
    15601 gttatagttt tcttttagtt cttttgtcag ctggttgtcc attttccaca ttccttttat
    15661 ggatattttt tcgatacttc cttattatac tggttttttg tcaaatttga aatggttcgg
    15721 tcaaaaggta agtaaagctt taataatcct agtgattgac gagaagttgt gaaaaattcg
    15781 tttagtttcg agtatgaaga aaaaattttg tgaaaatggc tttccatatt tttgcataaa
    15841 tttgacttaa taccgagaaa ctggactttg aacaccgttt attggagaag tcatgaaatc
    15901 agcgccttga cctgaatatt agagaaataa acagaaaaat atcaaaaaac cgaaaaagga
    15961 catcgccctt tttcggtcat ttttagtgat tatgaacgtg aatctgctgt aacagaaaca
    16021 tttgcccatg ggtttggtac atcataagac caatcccagt ctttgacacg gtcgttgact
    16081 ggcaatactt cattacggaa caacgttgga atcgcaaatg cttcttcaaa tgcatattgt
    16141 tgccattttt gatatgcttc tttttggtac tcttcatcaa atgctttgct tgaactgata
    16201 tcgttgatca attttgtatt ttcttctgat tcataacgag tgtagttgaa cgcagcattt
    16261 ggtccccata gtccacttgg atttggatca gttcctgtat tccaagcagc ttggtaaaca
    16321 tcaatttcag gatcatcgtt ttctaattta tcatagaaag aattgaattc gatcagacga
    16381 cctgttgtgt attggacatc caaaccaatt tctttccatt gttggatata gtaatctgcc
    16441 aatggttgcg ctgtttcccc accggacatc gaagcaaagt tgattgttag tttatttcca
    16501 tctttgtctt ctcttaaacc atcgtcatcc acatctttgt agccagcatc atctaatagt
    16561 tttttcgctt tgtctagatc ttgtgtgaat cctttcatct catcatcatg gtatgactgg
    16621 aatactggag gaatcaatga agtcgcattg cttctcaaac ctgcatagaa acgttcacct
    16681 actgcattat tatctaatgc atagcccatt gcttgacgta atgatttatc agccattttt
    16741 gaatctggat tgtattccac actctttgta tctgcattcc atttacctaa cttgaatcct
    16801 agataagtat aagaagtttg ttcacggcct aagtttgtat agccagcaat ttctttatag
    16861 gtatcgtaag tatctgttgg catttggaaa atcatatcat ataatttaga gttcaatgct
    16921 tcagttgcac ttgccatcgg tacagatttc atcgtgatct ttttcaattt aggcgtacca
    16981 ttccagtagt gctcgtttgg caagtattct actgattctc ctgcaacgat attactcatg
    17041 taataagggc caaaagtcac tggattttta cgtacttgat cgcttgattc catatctttg
    17101 attgctacac cttctaacgt atgttttggt gctgcatatg accagatacc accacccgat
    17161 tgaagcatgc ttgggtttac ttctttatat tcgatttcaa ctgttttgtc gtctattttt
    17221 ttgattccgg agattgtgtc ggcacttcca tcatgatatt cttccatacc tacgatgtta
    17281 cggaaagtgt cgtcataacg gataccagtg taatctttat tcccaatgat ttcgtaaggg
    17341 aaaatcacat cgtccgccgt tacatcttca ccatctgacc atttgacgtt gtcttttact
    17401 ttgattgtcg cttttttcgt atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg
    17461 attctgaaat cgtcatcgtt tgcaaatagc ggttcatgag aaggagccat aaaagctgca
    17521 tcatatgaat cttggtaaaa ctcccactgg aacaatcctt taaactgact gtccgttgcg
    17581 accgcagctt ctaattcgcc gtcttcgatt gcatcatcat tattttttgt tgcaattgga
    17641 aaagttaatt cctctttgtt attgccgttg cctccagagt tgtttccaga ggtatctgta
    17701 cctccgcccc cgcatgctgc aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc
    17761 gttttcttca ttcctatttc ccccttcgct tctatactag cctaaccgtt ggcgcgcatc
    17821 tgcagaacgc ttgaatgctt gtccaacata attgatactc aacatcaaaa ctaaaataag
    17881 gattgatgcg ggtaaccaga tccattgccg attcactaag acttctccgt tacttgcata
    17941 accgatcaat gtacctaaac taggtacatt tgccggcaag ccgaaaccta agaaagtcaa
    18001 agttgtctcg ataccgatgt ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga
    18061 acttagattc ggcatgattt cgcggaacat gattttaaaa tcattggttc ccattgtttt
    18121 tgaggcactg acataatctc ttcttgcttc agataatgtc ttgcttcgga ataatcgggc
    18181 tttagctacc caataaaatg cactcatgat gaatataaag gaccagacat tgtatctgga
    18241 aataatcgat acgaagacga taatgatcag catgatcggt agaatcatga taaaatcaac
    18301 gatccgcatc aagatgttgt caatgatccc tccgtagtag ccagagatga tccctaatcc
    18361 aaccccgatg atcgaagtaa ggatcgtgat cgcaaatccg atcgtaacag agtttctagc
    18421 acctatgatc aattgtccta atacatcacg tccgccttca tctgctccca gaatgaactt
    18481 ttgtccttgt gtcgaaactg ttcctggtgc agcgtatttg tcgaagatac taacatacat
    18541 cactttgtct tgatcggtca gcatcgcacc gataaagatc actaataata aaaccactag
    18601 caaacctaat gaaaaaatag caattttatc ttttttaaat tctcgagcaa tcattcgtat
    18661 ccccatagga ggaatacttt cttgaactgc tgtattgttt ttttcactca tcgatccatt
    18721 ctcctttctg attggtttta ttgaatccga atacgcgggt caacgatact catgatgata
    18781 tctgaaagta atgtccccac tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc
    18841 acggtatagt cccgttggcc gatggaatca ataaatagtt ttccgactcc cggataacca
    18901 aaaatctttt cgataactac agaaccagca atcaacgaag tgatttcata ccccatttga
    18961 gaagccatcg gcaacgctgc atttcggaaa atatgtcgac tgaatacttt attcgtcggt
    19021 acccctttgg aacgggctgt tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt
    19081 cgcagatatt ggattgtgac ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga
    19141 atatatttca aacggtctat ccattgagtg ataaatccac cttgcaaacc tagtgtttgt
    19201 gatccacttg ttgggaacca gcctaaacga taaccaaaaa caaatagcat gatcaagccg
    19261 aagataaata aaggaacagc aaaactaaag aagttgtaga ttacgacaat cttatccaag
    19321 attgaatttt gataccggcc agcaagcatc cccagaggca cagcaatcaa ataagtaagg
    19381 acaactgtca agatcgatag atataaagtg ttgatgatcc gttcgccgat caagtcagca
    19441 acagagcgtt tgtataagaa actttgacca aaatcgcctt gcaaagcatt tccgatccaa
    19501 cggaagtact ggacatacca tgggtcattc aagccagctg cttgtcgcat ctgttcgatc
    19561 actgccggat cctgatttgg attgatcaaa ccggtaaatg gatcccccgg catcgcttgg
    19621 gctaataaga agatcagaac acttaaaata ataacttgcg ggatcatcaa taaaacacgt
    19681 cgcagaatcg ttttccacat tagcttgcac ctccatcttt caacgccact ttgtgtgtag
    19741 ttgtgatcgt acgcaaatcg tagactcgac cagtcgcatc atagtattct ttttggtttt
    19801 ggatatactc ttgttctact cttcgtcgat tttctttgtg gctttcccta ttttctacat
    19861 cgatttgcgg aatagctgaa agcagacgtt tcgtatagat atgtcgcggg tcattataaa
    19921 tgtcttctct tgtaccgatt tctacaaatc gacctttgta cataattgcg atattgtcgc
    19981 acatgtgttt tactacacct aaatcatgtg aaatgaataa atagctgagg ccgtattctt
    20041 gctggattcg tttcatgaaa ttcaatactt gcgcttgaac agatagatcc agagcagata
    20101 caggttcatc ggctacgatc agttttggat tggtagcaac agcacgggct actcctagac
    20161 gttgacgttg tccgcctgaa aattcatgag gatatttata tagcgcatct tccggcatcc
    20221 caacgatatc tagcagtcct tttacctttt tcttttcttc ttgatcgctt aaccgttcaa
    20281 aatttcggat gggttcagca atgatatcca acactcgctt tttaggattc aaactggaca
    20341 ttgaatcttg gaagatcatc tgcacatcct tgttataacc catttttttg cggttgcttc
    20401 gcttcgttac atcttttcct tcatacatga tttgtccggc tgttaccttt tctaatccca
    20461 caacggcttt tcccgtagta gattttccag agccagactc tcccactaaa ccatatgtct
    20521 ttcctttttc gataataaaa tccacaccat ctacagcata tacatgatcc gttactcgat
    20581 tgaagaatcc actgcgaata gggtaatgaa ctttcaggtc tttgattgtg attaattctg
    20641 tcatctacac ctctcctttt tcgtctctaa agtggaaatg cttatagcag gagcaacgta
    20701 ccaagtggtt cggtgcgact tcatgaagga taggctcttc ttcatgatcg cttgctggta
    20761 tccatggaat acgtggtgca aaccgacaac ctttacgagg catgtttttc agagaaggaa
    20821 cgacaccttc gatcacatgg agttctgcat catgatttcc ttcttgtggt atagagttca
    20881 gtaaagagcg tgtataagga tgcttaggat gatcaaacag ttcttttaca cctgctactt
    20941 caacgaactg tccgccatac atcactgcca ctcgatcagc catttctgct acgactccta
    21001 agtcatgagt gatcaagatg atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat
    21061 caagaatctg tgcttggatg gttacatcta gggctgtcgt cggttcatct gcaatgacga
    21121 ttggaggttt gcaggcgatt gcgattgcga tgatgacacg ttgtctcata ccgcctgata
    21181 gttcatgggg atattggcgt ccaactctcg caggattagg aatccctact tgttctagca
    21241 attctagtgc acgagcttga cgctgttctg cattcatctt tgtgtgatag gaaagtcctt
    21301 ctttgatctg gtcttcgatt ctcatgagag gatttaatgc agacaacgga tcttggaaaa
    21361 tcattccgat atcatttccg cggattttat tatagagcgt ttcgttcagg tcgattaaat
    21421 tcagatcgtt atataaaatc tcacctgtaa tttttgtgtt attaggatca agtaatccca
    21481 taatcgtagt agccaaggta cttttcccgc acccagattc cccaacgatt gctaaaatct
    21541 cgtttttgtc taaagtaaat gaaacgtcat ctactgcatc gtaatagtca tctttgagtc
    21601 ggaatcctgt atgtaggttc tgaacttcta ataattggtt atcggacaat cgaatacccc
    21661 tctctgcttc ctaaatggaa gtttttctta ataaaaaagg acattacaca gaataaagcg
    21721 ttttataaca aaggttattc ttatctttgt gtaatgaatt attaataatt atatagaaaa
    21781 tcgctttact ttgcaacata atttctgttt atcgccagaa ttatcagaaa aatgactttt
    21841 acgtgtcaaa aagattaaaa aaagaaggat aaaaagctgt atgcagcttt ttatccttct
    21901 ttgcttttat ttgaaattat ttaagatggc tgctgatata atctactgcg ccaccgactg
    21961 tttcaatttg ttcagcgtct tcatctgaaa tttccgtacc gaattcatct tcaagctcta
    22021 aaacgaattc cataatacta attgaatcag cttttaaatc atctttgatg ttcaattcat
    22081 ccgtcacttt atcggtatct atctcaaaat gatttgaaat aattttcgct actttgttaa
    22141 atacttcttc gcgagtcaac tttttcacct ccaagaatca tacacttgtc aattacctac
    22201 accatggcta attttactgg catttttcag gaatgtccac ctttttttca atggaacgga
    22261 ttgcttcaga tatccaaata ctattcttcg cttttttcaa attgttcgac taattgtcca
    22321 actacatctg tctcaagcat tgtgtggatt tggcggatcg tatagcgaac agcatcgggg
    22381 cctgttgctc catgagtttt aatgactggt gctttcaagc caaacaaaac ggcaccgcca
    22441 tgtttggaat aatccatttc tgctttcaaa gaacgtaagc cgtcttttaa aagcatagcg
    22501 cccatttttc ctttgatccc ttcatttaga atagcagatt ttaatagatt catcatattc
    22561 attgctgttc cttcaataga tttcaaaaca gcgttgcctg taaatccatc tgtgaccact
    22621 acatcagcca caccattgag tagctcacga gcttctacgt ttccaataaa attcaagctt
    22681 gtttcattct gaaggagttc ataggctttt ttcgtcactt cactaccttt tgttgcttct
    22741 gttccgttgt ttaagagtgc gacacgagga cgtccgatac ctcggacttt acgtgcataa
    22801 aaagaaccta aaataccgta ttgcaacaaa tgttctggtt tgttttctgc gttagcgcca
    22861 agatccagca tatcgaaacc ggcaccttct ttccctacta ctggcaaggt agacatcaag
    22921 ccagggcgtt cgatattttt gatacgtcct acgatgaaca accctgctgc tagtagcgcg
    22981 cctgtattcc ctgctgaaaa tacagcatct gcttcaccgt ttttcactgc ttgtgcagca
    23041 aggaccattg atgcagtctt ttttcgtcga attgctttta ctggctcatc atcactattg
    23101 attttttcgt ctgtatggat aatcgtgatg tttttttcat ttgttaagta tttcttgatt
    23161 tctgcttctt ttccgtaaag taagaattct acttctggaa aatcttgctg cgctaaagcc
    23221 acgccttcta caattgcttg aggtgcgtga tctccgccca ttgcatctac ggcaattttc
    23281 attgtttcca ctcctttttc cgtcatcagt ataaagtata gcacagtctg ctaatcgaaa
    23341 taacgattat cctgattttc tatttggagg ctttgcgcta aaggctgata ctcaggaagc
    23401 atctgccaat cctttatctg ccaaagggct gcagcctctt ctctggctac ctccaaaaca
    23461 tttccatccg tcaccaagtc agcaatcgca aattgaggca gaccagattg acggaagcca
    23521 aatacttcac caggaccacg taattccaga tctttttcgc ttagtacaaa accattggtg
    23581 gtctctgtca tgattttcat ccgttccaca cctagttcat ttttaggatt agcaactaag
    23641 atacagtagg aagcatcact tccgcgtcca acacgtccac gaagttgatg cagctgtgcc
    23701 aatccaaatc gatccgcatc catgatcagc atgacggtcg cgttcggcac gtttacccct
    23761 acttcaatta cagtagtcga aactaatatc tgcatctggt tgtctttgaa ggcttccatg
    23821 attacttctt tttcctgatt cttcatttta ccatgtaaca agccaacttc atattttggt
    23881 gcaaatagtt cacgaagttt ttcatagatt tccactgcat tttttacatc taatgcttct
    23941 gatccttcaa tcagcggaca gatgacatac atttgatgtc ctcttgccag ttcttttttc
    24001 gtccaatcca gtacactgtc caattgaggt gttcttaccc agcgtgtttc gatcgggata
    24061 cgtccagctg gaagttcatc aatgatagat acatccatct caccatatgc tgtaatagct
    24121 aatgttctag gaatcggagt cgctgtcata aataagacat ctggtcgcca gcctttttca
    24181 cgaagtactt tccgctggtt cacgccaaaa cggtgctgct catccgtaat aactagacct
    24241 aaattctgaa attcgactcc atcttggatc aatgcatgtg tgccgatcac gatgttaatt
    24301 tccccttgct ctagtttttc aaggagctca cgacgttctt ttgctttcgt tgaccccgta
    24361 agcaatgcga tggttacttc attgggatca aacagctgtt gcaagctttc catatgttgt
    24421 tgtgccaaga tttctgtagg caccatcaac gcaccttgga aacctgctgt aacggtagcg
    24481 tataacgcaa tcgcggcaac cactgttttt ccgctaccta catctccctg caaaagtcgc
    24541 tgcatatgct gcggactgcg aagatcccga cagatttcat tcgttacacg tttttgtgca
    24601 tttgtcagtt caaaaggtag cttttgagta aattctttca atttttggac atcataaagg
    24661 atctccaaac cattcgtttc tgctttttct gcttttttca atccttgaag acgcatttga
    24721 aataaaaaga actcttcaaa aacgacacgt cgtttggctt gatggttttc ttcgtggtct
    24781 tttggaaaat gcatacttct tacagcagaa gctcggtcaa gcagtcgata tttctccaca
    24841 aggttgtttg gcagattttc ttcaatcaat gtgccgtatt cttgaaaccc ttttttgatt
    24901 agatcaacta atgtcgtttg acgaattgat ttgttgacgt gatatatcgg cgaaaagtca
    24961 tccattgttt gagaacctaa aatcttcatc ccgttcaatg ctttacgctt tgcatcccat
    25021 ttaccgtata cggcgatttc ctccgataaa atgacttttt cttttagata aggttgatta
    25081 aaaaaagaaa cgttgaacac atcatgttct tgcatcatac gaaattgcaa tcggcttttt
    25141 ttataaccaa atcgattcat cacaggaggc gagactacga ttcctttcaa agtaaccttt
    25201 tcttgatctt ggatctcgtt tagatttctt tcctgaatat cttcgtagcg gaaaggataa
    25261 taagtcagta agtcttcaat cgtttggatg cccaagctgg caagactagc tgctcttttt
    25321 tctccgactc ctgccagcat agtgattgga tcatctaaag aacgcataaa tttttcctcc
    25381 ctcccttaac aaaaaaagga cttggtcagc gatcatacct gatcactaac cgaaggcctt
    25441 ttatgaatga atactgtgtt agaaagatat tctgacacag aaattattca gcagaaaata
    25501 gataaggata gacaggctga tctccttggt ggatctcgat ttccaaatca ggatgttttt
    25561 ctgttagttt ctcagcaaat tcatttgctt gttcaactgt tccttcatca cctgccaaga
    25621 tcgttacgat ctctgtgtcc tcattgatca tttgttctag tgtacttaac gctgttgctg
    25681 tgatatctgg gttagagatc aggatttttc catcgatcat gcccaaaaag tctccttctt
    25741 tgatctctac accgtcaatt gaggtatctc tgatcgcgtg agtgacttga ccgctcacaa
    25801 cgctagacaa catttctgtc atcgtttctt tgttttcttc taaagaagct tgatcattga
    25861 atgaaagcat agctgtcaat ccttgggaaa tcgttcttga aggaacaact gctactggga
    25921 tatctgctac ttctgctgct tgatcagctg ccatgaagat gttcttattg tttggcaaga
    25981 caatcacttt atctgcgttt acttcttgaa tcgctttaac aatgtcttcc gtacttggat
    26041 tcattgtttg cccaccactg ataacataat tagcgcctaa actttcaaac agctcttgga
    26101 cgcctttacc agcagcgatc gctacgatac catacgggat acgtggtgtt tcttttttcg
    26161 caaactcgtt cacttcttca tcgtgttcca aaatcgtctc atgctgcaag cgcatgttgt
    26221 cgactttgat tttgactaat gaaccgaatt tttgtccata attcaagact tctcctgggt
    26281 tttctgtatg cacgtggact ttgatgatct cgtcatcatt gaccacaagt aaagaatctc
    26341 ctataccatc taaatagtta cggaattctt cgtaatcgaa tgtactatcg actgtaggtc
    26401 cttctccgat ttttaccatg atttctgtac agtaaccaaa atggatatct tctgtcgcca
    26461 tttgaccttg gacactgcga tgatgttctg cattgaccat atcatccatt tctgctggag
    26521 aaggttcata tgtttcatcc gcttggaatt ctccattcaa tgcacttaaa aagccctcat
    26581 aaacaaacag cagtccttgc cctccactgt ctacgacacc gacttctttt aggactggaa
    26641 gcaagtctgg agttttagct aaagcacgct ttgccccttt tacaacagct gtcattactt
    26701 caacgacatc atcagtactt tttgctttct tttctccagc tttcgctgct tctctagcta
    26761 ctgtcaaaat cgttccttct actggtttca ttactgcttt atacgcagtt tcaactccat
    26821 gagtgaaggc tgcagcaaga tcggaagcat ttaatgagac aacatctgga atttgcttag
    26881 aaaatccacg aaacaattga gacaaaataa cgccggaatt tccgcgcgca cccatcagca
    26941 atccttttga aagcattgct gctaattctc ctactttttc tgaggttgaa tctactactg
    27001 ccttagctcc actagtcata gatagattca tatttgttcc cgtatcgcca tctggcacag
    27061 ggaatacatt taatgaattg acgtattctg catttacttg cagacgattc gcacctgcct
    27121 ggaccatttc ctggaactgg cttgcactta tttctgttac actcacctag ataatcctcc
    27181 ttcagatcga ctgctgagtt accagccagt ctcctgccta gtctggcagt acacgaacac
    27241 cttgtacaaa gacattaact gaattggcag ttacgcctaa taatgcctct agattgtatt
    27301 tcactttttc ttggacgttg cgggaaactt cagagatttt cgtaccatag ctgataatcg
    27361 tataaacatc tacagcaata ccattatctt cttggcggac aactactcct cttgagtaat
    27421 tttctttacg caaaatctca gtaatattat ctttaatttg atttttgctg gccattccta
    27481 caacaccgaa cacatctgtc gcggcgccac caacaactgt tgcaattaca tcattggtaa
    27541 tttcaatggt tccagctgac gtttgaattt ttacagccat gaatgagcct ccttaacggg
    27601 taaaaaactc ccatttttct tctatcatat tttatcatag cgcaacttga aataaaaggc
    27661 agttacccaa agtactcaaa aaccttgcaa aatttcaggt ataaccttgt ttaatttaca
    27721 attattcttc tcaattgtca attgtatcta tgaaaatgta cttgcaaatt tccattctat
    27781 atgatatgtt agtctggtat gagctgaatt attctattgc tccaaatcaa ggcaaaggag
    27841 gaaattacca tggcaaaagt ttgttacttt acaggtcgca agacaaaaag tggaaacaac
    27901 cgttcacacg ctatgaactc aacaaaacgt actgttaaac ctaacttaca aaaagttcgc
    27961 gttatggtcg acggaaaacc taaaaaagtt tgggtgtcaa ctcgtgcttt gaaatcagga
    28021 aaagttgaac gcgtttaatt gaatgatact tggaccgttc agatagtgaa cggttttt
//
LOCUS       DAYYGS010000031        27896 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000031 DAYYGS010000000
VERSION     DAYYGS010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.031"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..705)
                     /locus_tag="ACHA44_001249"
                     /pseudo
     CDS             complement(7..705)
                     /locus_tag="ACHA44_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            784..960
                     /locus_tag="ACHA44_001250"
                     /pseudo
     CDS             784..960
                     /locus_tag="ACHA44_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1136..1705)
                     /locus_tag="ACHA44_001251"
     CDS             complement(1136..1705)
                     /locus_tag="ACHA44_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8904552.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1779..3068)
                     /locus_tag="ACHA44_001252"
     CDS             complement(1779..3068)
                     /locus_tag="ACHA44_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904553.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3395..4270
                     /locus_tag="ACHA44_001253"
     CDS             3395..4270
                     /locus_tag="ACHA44_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8904554.1"
                     /translation="MGKRSTIQLMIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGT
                     FSRVQCTPDLLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLE
                     AMAENHATIDNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDD
                     ELLLLLDKFEKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAI
                     QLGISPRGSLSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKK
                     QIIHTLVAQIPIPVR"
     gene            4284..4607
                     /locus_tag="ACHA44_001254"
     CDS             4284..4607
                     /locus_tag="ACHA44_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904555.1"
                     /translation="MEKIKDYFINFVLFLVYFIIVLFALVFNNSLGWFLLAFFTLYVS
                     WSLISLSSFLKKLSVTCTLQPLVYRKKSMQVIFSIAKKRTFSLPLPRLQIGFPAIFAE
                     KNKRF"
     gene            4604..5356
                     /locus_tag="ACHA44_001255"
     CDS             4604..5356
                     /locus_tag="ACHA44_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HFM8904556.1"
                     /translation="MILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFRKSAKR
                     EISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNRIDWKL
                     SAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPELLILG
                     NRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIETLSKD
                     RTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5346..7517
                     /locus_tag="ACHA44_001256"
                     /pseudo
     CDS             5346..7517
                     /locus_tag="ACHA44_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7629..9800)
                     /gene="copB"
                     /locus_tag="ACHA44_001257"
     CDS             complement(7629..9800)
                     /gene="copB"
                     /locus_tag="ACHA44_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HFM8904557.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9820..12006)
                     /locus_tag="ACHA44_001258"
     CDS             complement(9820..12006)
                     /locus_tag="ACHA44_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8904558.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12006..12215)
                     /gene="copZ"
                     /locus_tag="ACHA44_001259"
     CDS             complement(12006..12215)
                     /gene="copZ"
                     /locus_tag="ACHA44_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HFM8904559.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12228..12668)
                     /locus_tag="ACHA44_001260"
     CDS             complement(12228..12668)
                     /locus_tag="ACHA44_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HFM8904560.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12742..13281)
                     /locus_tag="ACHA44_001261"
     CDS             complement(12742..13281)
                     /locus_tag="ACHA44_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HFM8904561.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13896..15362)
                     /locus_tag="ACHA44_001262"
     CDS             complement(13896..15362)
                     /locus_tag="ACHA44_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFM8904562.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15673..17754)
                     /locus_tag="ACHA44_001263"
     CDS             complement(15673..17754)
                     /locus_tag="ACHA44_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8904563.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17896..18018)
                     /locus_tag="ACHA44_001264"
     CDS             complement(17896..18018)
                     /locus_tag="ACHA44_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904564.1"
                     /translation="MNLDKLPATGFKLSCYPVKIKKASAGWIRAVAMIEKKKKE"
     gene            complement(18278..18637)
                     /locus_tag="ACHA44_001265"
     CDS             complement(18278..18637)
                     /locus_tag="ACHA44_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFM8904565.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18667..19008)
                     /locus_tag="ACHA44_001266"
     CDS             complement(18667..19008)
                     /locus_tag="ACHA44_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904566.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(19005..19679)
                     /locus_tag="ACHA44_001267"
     CDS             complement(19005..19679)
                     /locus_tag="ACHA44_001267"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFM8904567.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20732..22030)
                     /gene="asnS"
                     /locus_tag="ACHA44_001268"
     CDS             complement(20732..22030)
                     /gene="asnS"
                     /locus_tag="ACHA44_001268"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFM8904568.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22070..23260)
                     /locus_tag="ACHA44_001269"
     CDS             complement(22070..23260)
                     /locus_tag="ACHA44_001269"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8904569.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLSVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23281..23808)
                     /locus_tag="ACHA44_001270"
     CDS             complement(23281..23808)
                     /locus_tag="ACHA44_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HFM8904570.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23855..26644)
                     /locus_tag="ACHA44_001271"
     CDS             complement(23855..26644)
                     /locus_tag="ACHA44_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HFM8904571.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(26791..26991)
                     /locus_tag="ACHA44_001272"
     CDS             complement(26791..26991)
                     /locus_tag="ACHA44_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8904572.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(27443..27763)
                     /locus_tag="ACHA44_001273"
     CDS             complement(27443..27763)
                     /locus_tag="ACHA44_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904573.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
BASE COUNT         7842 a         5917 c         4896 g         9241 t
ORIGIN      
        1 gattttttat cgttgtggaa tatttaaata ccaacgcttg tcatgaaaat cttgtgctcc
       61 acccttagta tttccttctg gatcgtttgt cgcacgcatc atgacgtata ctttcttatt
      121 agggaaatca cgcatattga aagatacatg atagccaaca tttcctaaag tattataagc
      181 ttgatttacg tctggtctat aaatcccatc agctcttact ctagctagtt cttttccagt
      241 attgtagtcc ataatgaaaa tatactcgta tttataatta gcaatgtgcc atccagctac
      301 atgcaagttt gcgttttcga tttctccgaa ctgatcaatg tgggcgtaat ttgttccatc
      361 tgtcagcgta gaatttgcag cacctgctcg agttggatca attactggtt tatcctccga
      421 agtagttgga ttatcatccg taaaaccatg agccaaatca taggctagtt tttctttact
      481 tacacccatt tcagaaagat aaccgtaagg atctgtatga tcgccccaaa tattttgtgt
      541 tacccataaa tgcgatttga ttcctggttg gttataagga gtgtctaatg ttaatggaat
      601 accatatttc attgctgaat ctcttgccaa ttcaacgtat actttatagt tcttttcaaa
      661 agtttcttta tcatgtgtgt gttgtaactc gatttgcaca ggacttttgg ctcacgaggt
      721 tgtcctactc tacatcttgc tgatagatat gctgaaagga aaacaccaac attatgtttt
      781 gctttttcag ctagaacgag acaagcagga atacctttag aagatatcaa agatttttta
      841 ggtcataaag atgtttccac tacacaagtc tatgcccata tttctcccga agtgaaaaaa
      901 agatcaatga atcaactaga aaactatata gaagagcaaa taaaaaagca ctcaaattga
      961 gtgctttttt aagctcacta tcactaaaac tatcactaat tttatttata agcgaacatg
     1021 tttctatcac tgatttttta aatctagcga aaaaccctta tcttaaaggc gttcgttcaa
     1081 ttctttggca aggtcttcaa agcctggctt gcctagaagt gcgaacatgg ttttcttata
     1141 gctcatgtag aaaaattttc ttgatgatga ttttatccat caattgagat tgactgtttt
     1201 gctctcctat gatttcagct atgattgtcc gattttgcgg ttttcttctt aactggtagt
     1261 tgattttccc agtttccatc aactcgctaa gcatatccac gactccgcca aatccggtct
     1321 ttttcgtaat aaaacctagt gctaccattc gttcatctag attttttgct gtaatcgtca
     1381 caccagtaat gagcgagtgc tgtaatgttt gggccagctc ttccatccct tcagcattgg
     1441 atacgacgga agctgttttg ttaataggtt cttcgtttat ttcttttggt tctttcctct
     1501 ccactttcac tgtttgacga aggacatcta gttcttgcaa gagcgttttg tattgttctt
     1561 gggtcagcat cacgccagct actttttcac gattaaatat atatacgcca gttcccgcgt
     1621 ctcgcgcttg agcaaataca tccatcggcg atcgtttcac ttctgtgatc gaagatgtgg
     1681 gaacttctaa tttctttaat tccaatggat ttcctccttt actaatcgct ttttattata
     1741 gcacagactg acttgttttg atcttttttc caaaggtttt atcggttttt gcctaaccag
     1801 taattgatat cggtaagaag tctttcgata ccaaatgaag gatggtacag atacgttttt
     1861 tgatcttcgt tatggtctag tggaaaaaaa tgatcggtga tcactagatc tgcataccga
     1921 ggcggcttat ttcgagtaca tacatgtata gagatccgtt ttcgatgtct tagcgttttg
     1981 ttcagtaccc cagcaagtct tcttgcttct tggtgttcat agcgaaaaaa aaagacgatt
     2041 ttcacttcag gagggtggac atctaaatac cctcgttcct ggagtgtttt cagtaaaagc
     2101 atcccccact ctactgcggg gtgtgcaagt gcttgttgga tcgctagccg aaattgatat
     2161 agtttatctt tggaattggg aaatttttct aatgtttcct gataataacg aacttcttct
     2221 atcagccggt catggatacc aattacgtaa cgaagccatt cttggtaaag tacttttatc
     2281 agctttgatt tgctttcgct ctcaaattcg ctattcagcc aaaacagcga atcaagcttt
     2341 tcagtcacaa tcgtcaattt ccgatagtct tgtgtctctt ctatccgttt ccaccgcttt
     2401 tcagtaatct ggttacagct caagcgcaaa attgctagat aatttttttc ttttattggc
     2461 aaaatctgct cctctctaaa tagcaattca ggaaacaatt caaagaatag tgttcgactg
     2521 tctcctaaaa tctcgatttc tgccggatcc tttctttgct tcaataattg gatgaacatc
     2581 tcagaaaagg actgctctcc atcatctgca aaaaaatgcg gaaaatcttt caaaaagtaa
     2641 aacagcagat atcttacttt tctttctgaa ccaaagatct gtgctttttt ataagaaaaa
     2701 gaaaggtggt actcgccaaa ccactttctc aatcgctctt gcgtagaacg atcgtacttc
     2761 tgctcgtaag gttcatttaa tagcagattt ttcagtaaac aaaacggggg tgattggatc
     2821 agaaaaacaa acaagagata aggttcgcta tatccttgat cataaactaa ctgaacggtt
     2881 ttcttatgaa gaatcagctg aatgggacag tcgctttccg ccaaacgttt cagatgccga
     2941 tccactgttt taacatcgat tttcagttct tcagctaaat cagcttttcg ccagatcaaa
     3001 tctttcatta gattgattag tgaaaataaa ttctgtgcat tttttgataa tagtttttgc
     3061 catctcatcg atcatttctc acctgatttc taaacatatt gtttgatcaa aatgagttca
     3121 ctcctaaaaa gataaaaaac tccctttcta tttatttaaa tacgcaaatc ttcctcatta
     3181 ttttttattt tactcaattt aatcgtattc tcatttcaaa acgaatgtac tttaaaaaaa
     3241 cataataaga tcactttgct tgtaggatgt attcccttcc attacaatga aataaatagc
     3301 gattttatga gaaggtgatg tgaaaatgga agaaaaaata gaagaatcca aacaaaaaat
     3361 cgatcagttg atataagaat tagaaaaagc ggtcatcggc aaacgttcaa ctatccaatt
     3421 gatgatcgtg gccctattag caggtggtca tgtcttattc gaagatattc ccggtgtagg
     3481 taaaacgtta ctggtcaaag cactagcaaa agctgtacag ggaaccttca gcagagtcca
     3541 atgtactcct gatctgctgc ctagtgatat tcttggtttt tccgtataca attcacaatc
     3601 aaaagagttt gaatttcgtc cgggaccgat ctttaccacg atcttgttgg cagatgagat
     3661 caatcgtacg actcctcgta ctcagtcagc acttttagaa gcaatggccg agaatcatgc
     3721 aacgatcgac aacaatacat accctctaga caatcatttt tttgtcttag ctacgcaaaa
     3781 tccaatcgag tacgaaggaa catatccttt gccggaagca cagctcgatc gttttttatt
     3841 ccgactacaa atcggttatc cttcttttga tgacgaactt ttgctacttt tggataaatt
     3901 cgaaaaagtg ttggataact taaatgaagt attgaacagt tatgaaatag aagaaataaa
     3961 aacaaatgtg gcgaatgtct ttgtcagtcc cgaagtggca tcttatgcct tgcagttagt
     4021 gtcagcaaca agaagtcatg ctgccatcca gcttggcatc agtccacgag gaagcctttc
     4081 ctttattcag gcagcaaaag cttatgcttt aatccatggg agaaattacg tcacgcccaa
     4141 agatttgcag gatttagttc cttacgtgtt ttcccatcgc ctgatttttt acgatcgttc
     4201 attgaaggaa gacgaaaaga aacaaatcat tcatactctt gttgcacaaa ttcctattcc
     4261 tgtgaggtag attgaggtag acaatggaaa aaatcaaaga ttatttcatt aattttgtgt
     4321 tatttctggt atatttcatc attgtgcttt ttgctcttgt attcaacaat tctttaggct
     4381 ggttcttgtt ggcctttttt actctttatg tatcatggag cttgatttca ttaagcagct
     4441 ttctaaagaa actttcggtt acttgtacat tgcagccact cgtttatcgc aaaaaaagta
     4501 tgcaggttat tttttccata gcaaaaaaga gaactttttc tttacctctc cctcgtttac
     4561 aaatcggctt cccagcaatc tttgcagaaa aaaacaagag attttgattc ttacaaataa
     4621 accgcaagaa gtccaattgc tttggcagcc aaaagaaaga ggatgccatg aaacattaac
     4681 tgtcattttt acagcctatg atagtctagg acttttcaga aaaagtgcaa aaagagagat
     4741 ctcttttcct gtgactgttc tgcctgcttt ttgtaagatt caagcagaaa aattacagag
     4801 agttctggaa aaaaaacagc agctgaacgc gtaccaaaga ggtcttgatt ttcgggaaca
     4861 tcgcgcttat cgtccaggtg attctttcaa ccgaatcgat tggaaattgt ctgctcatac
     4921 tcaagagtgg ttataccgtg aatacgatta tcaagaagaa gaatgttctc ctcttttttg
     4981 cttttggggt tcgcccagtc caaaatttga acctctgcta gacttgtatt tttctttatg
     5041 gcatttgaga aaagaaaagc agccggaact tctgatatta ggcaaccgcg cctttcatgg
     5101 caaagacccc ggtcatacct attttgcttc tttggaagct ggcgatgaga aagctttttt
     5161 cgcacacttg aaaaaatatg caaaaaaaca aatcgtcctc tttgttcctg agaattctcc
     5221 tgaagtctca gaagctattg aaacattgtc aaaagatcga acggcttatc ttgtttattt
     5281 cgtagaaaaa caactcatgg tccaagaaaa agacaagata tgttcattgc caggaggtga
     5341 atggattgac gattaaacgg gaaaaatggc tgcgtggcgt ttactccttt gtgatactaa
     5401 gtgtaggtgc tccgatattt gtagtagcta atcaattgca aaacagctac cttcttatcc
     5461 taactattgg tgtcctatgc cttattgtct gctgtattcc taaaaaatgg gtacgtttgc
     5521 tgctttcttt gccagtaatg ataggttgtt tgtttttata ttttccttcg aagacatttt
     5581 ccatattatg gatcttacag tttttttcgt cagttacaga ggaattcttc catctatccc
     5641 aaggtaaact ttattatttg ccagaaaaga cagctttttt tctcataatg ttgctgatct
     5701 atttatttat tacattgacc gtggattacg actattggta cgcacccttt ctttctttga
     5761 tggcttactt catgatgctt accgtttttc ggaaaaaaga tttactgtac gaaatgattg
     5821 cggtagtaac tgttttgttc gtgtatctgg cagcaaggca atttttctcc atccggttgt
     5881 tgtcgggaaa cactcgattc cagtcattga ctacagttct gcttatttgt ttcttaactt
     5941 ttagcgctat cttgccttta tatcttccga aagtacacca atctctcgag gaaacgagtg
     6001 aaccgatccg agaatatctt aatgatgaag ggctttatga cacactccat gagtatcaat
     6061 taaccggaag tgcaagaaca ggcttcagtg agaatgatga acagcttggt ggacctttat
     6121 atgataatgg cgaagttgtt tttacagcga accaaaaagg aaatggctat tggaagatcg
     6181 aaaataaaaa tcattacacc ggttctggat ggatagaaga tcaggacgca cccatccaac
     6241 caatccaaga attccctgtt gacatcacga gctaccaaac cgttatagaa gacaaagaaa
     6301 acattacatt gaccctaaaa aaacaagctt tcttacctta tccttatgga aatactacct
     6361 tgactgattt ttcattagct agtggacaaa cgctttacta tttgcctgaa agccagcgat
     6421 tcattcctgt cccgaatgaa tcgacacgtt ctgcaacttt acaaacagaa aggttagttt
     6481 ttacgagaga agaattaaaa agcagtcagt taaaagctga tgatttagaa aatcagcaga
     6541 caaatcttca attgccaaaa agtttgccta aacgtattga ggacttggcg gtcgaattga
     6601 ccaaaaatga agcaacttta attcgtaaag tcgaagctgt ctaacagtat ttaaaaacaa
     6661 gcggaaattt ccgctattcc aaaacggata ccgggcatac tccgaaagaa cacgattatg
     6721 tggattattt cctctttgat tcaaaagtcg gctattgtga caacttttca acttctatgg
     6781 tcgtccttct tcgaacactt ggtatcccag ctcgctggac aaaaggtttt acaaatgggc
     6841 tcagaacagg cacagataat caagggaatg aggaatacag cattctcaat agtcatgctc
     6901 attcttggcc agaggtatac tttcctggat ttggctggct tccatttgaa ccaacccctt
     6961 catttgccaa tccaatgggt tcacaaccag ttgctgaaac aaccgaaacc gtccagcctg
     7021 ctatcgaaca accaaatgaa agcgaaaata tggcagaaac tatttccagt gagagatctg
     7081 aggtcattcc atcagaagaa acaacactca caccagctta taaaatcgtc ttattcagcc
     7141 ttggagtgtg tttatccgtc ttgttgttct ttctattgtt attcagaaaa tctctgcgct
     7201 ggacaagtta tcttatctat cttcattttt ttgcttctac taatacgatt gcatatcaaa
     7261 aacttcttaa ccctttggaa acattggtac cgcgcaaacc cgcagaatct ttgcaggaat
     7321 atagcctgcg aatagaacaa gaattcaagc tagatctgcg tttcattcga ttaactactc
     7381 aatatgaagc agaaatatat ggacgacaag cagatccggt aaaattcgat aaaaaaacct
     7441 atttagaaat aacgcgtata ataagaaaaa cggtatacaa aaaaattctg acaaaaggga
     7501 gccgcttcaa aaaataagat aaaatttctg gagaatctac ttctggaatg ccgagaacca
     7561 gctgattgag tatcgattac tcgaaaaaaa ggttgtgaca cgttttttgt cacaaccttt
     7621 tttcattatt atttgatttt caatgtcaaa gcatttagag ctacaactac cgtacttaac
     7681 gacatcaaga cagcaccaac tgctggactt aatatgatac caattggcgc aagaattccg
     7741 gctgctagcg gaattgcaag aatattgtaa cctgcccccc accagaggtt ctggatcatt
     7801 ttctttcgcg tttgcttggc tagatcaaga aaatgcaaaa tatcttttgg atcgctgttt
     7861 gtcaatacga catccgcaga atcgattgcg atatctgtcc ctgcaccgat tgccatcccg
     7921 attgaagcac gggctaaact tggcgcatca ttgatcccat cacccaccat gattactttt
     7981 ttcccttggt caaggtattt ctggacaaca gcttctttat catctggtaa taaaccgcca
     8041 taatattctt cgatccctaa ataatctgct actgcttggg cagcgttttt attgtcacca
     8101 gtaagcatga caggagtgat ttttctttct ttcaatgttc ggataaattc ttttgcttct
     8161 gtttttacta cgtcacctaa agcaacgatt gcagctagat gattttctaa aatcaggaag
     8221 ctgatggtat ttccttgttc ttggtaaggt ttcaataatg cttgttctac gtttaatccg
     8281 agacgctcaa cttctttttc attaactatt ttcacttgtt gttttttgac tgttgcctct
     8341 agcccgactc ctgaaagatt ttttagattt tcagcttgat aaggcttgat ttcatgttct
     8401 ttcaaataat tcataatgcc aacagccaat ggatgattgg cattcgcttc caaggctcct
     8461 agatattgaa ggatttcatt tttattgaac gcatcatcaa gtacttcgac acccgttaca
     8521 gtaaattttc cttctgttag tgttcctgtt ttgtccagca tgatcacatc tagatcattg
     8581 gcttgctcca gtgcattgcg gtttttcaaa agtaatccgt ttttagctgc tattgatgtg
     8641 gagcgagcaa caactaatgg gatagctaaa cctagagcat gaggacaggc gatgataaag
     8701 actgttacca tacgttctaa tgcattagga agatctgtca agaacaacca ggcgatgaat
     8761 gccaggatac ccacaattaa tgcgacataa aacagccatt ttgccacttt gtcagataat
     8821 gactctagct tggatttttc tccttgtgct tttcgcacca tttccataac cttcgctaga
     8881 taaccatttt caccagttcc agttacttcg atttcgatcg ttccatcccc attgatagaa
     8941 ccgccaatca ctttgtcatt ggcttgtttt ttgattcctc ttgattcgcc agtcacagct
     9001 gattcgtcaa caattgtttc acctttcaag attacaccat ctgttggcat tttatcacca
     9061 gaccggacga tcaaacgatc accttcgttt acttctttca atgaaactgt ctcttctttt
     9121 ccatctttcg tcaagcgttt cactgattct ggcagtaatt ctgctagttt ttgtaaagca
     9181 ttgctggcat tcgacacagc attcatttca atccagtgac ctagtagcat aataacgatc
     9241 aaagtagcca attcccagaa gaaatccatg acgtgtgtat gtggattgag cagatttgcg
     9301 ataaacgaat agacactata aatatatgca actgtaatcc ccatcgcgat cagtgtcatc
     9361 atagcaggac ttttttgttt caattccatc ttagctccac ttaaaaatgg ctgaccgcca
     9421 tagataaata gtatagttgc taagatcaat acgagccaat cagaaccagg aaacgttact
     9481 tgaaatggaa attccattcc catcattggt gaaaaaagaa tgattgggat agctaggata
     9541 agcgatagcc aaaatttttg tttgaaattc cccatgtgca tggaatgatc catgccgccg
     9601 tgttcgtgat ccatgtgcat cgaatggtcc atttgtttgc tgtttgctat atctccatgg
     9661 ttatgtccca tgtgcatgga atgatccatc tgactatggt catgtttcat ctccatctgt
     9721 ttttcatgag attctgtact ttctttgttt actacttctt ttttatgctc atgttttttc
     9781 tccatatcgt tcccattcat ttttactcca tcccttctct tactttttca tgtgtcgatt
     9841 taagctcagg gagttcaaca atacactgac ggaactaaac gccatcgctc ctcctgcaat
     9901 gattggattc aaaaagccta gtgcggcaaa aggaatacca atcgtattat agacaaaagc
     9961 ccagaaaaga ttttgtttga tttttctcaa tgtcaaccta gacaactcga ttgtttgtgc
    10021 aatggatgcc aaactgctat tcatcagcgt cacatcggct gtttccatcg caatgtccgt
    10081 tccacttccc attgccatac cgacatcagc taatgctaag gccggtgcat cattgattcc
    10141 atcgcctgcc ataccgactt ttttgccatc tttctgaagt ttttctacgt aggctgcttt
    10201 atcctctgga agtacttcag caaagatatg ctttggatca ataccaactt gtttaccaat
    10261 cgtttcggct gccagtttgt tgtctcctgt cagcataaaa acatctaatc ctttattctg
    10321 gagctgcttg attgcttgtt tggcttctag tttgatttga tcagccacgg caatccaccc
    10381 gatcacttgc ttttcatcag ctaaaaacat gactgttttt ccctgctgtt ctaattctaa
    10441 cgcgtgttct tggtattcat caaatgatag gttcaattca attaatcgtt tacgtgtacc
    10501 cgcaaaatag cgtactccat taatcgtacc gctgatccca gcacctgggt gggctgtaaa
    10561 atcaatcata tcgtatgttg ctgcttcttg cagttttccg tattccacga ttgctttacc
    10621 tagtggatgt tcagaagctt gttctaaagt ataaaaaata gataggactt cttttgaacc
    10681 gactagatct gtaactttgg gctttccttc tgtaatcgtt ccagttttat ccaaaacaat
    10741 gctgtctaag tgagcagctg cttctaatgc ttctccgcct ttaattaaaa ttccatttcg
    10801 tgcaccaaca cccgttccta ccataatagc tgttggggtc gctaaaccta atgcacatgg
    10861 acacgcaatg actaatacag agacactatg gatcaatgcg agttgccaat caccagtgat
    10921 caaacctgta gctatgagcg tgataaacgc gatgactaat acgatcggaa caaaaatccc
    10981 tgagatccga tctgcgattt tttggattgg ggctttgctc ccttgagcat cttccaccat
    11041 ttgaatgatt tgcgctaata ctgtctgctt gccgacttgt gtgacttcca tgtgtagcag
    11101 accattcgta ttgattgtcc cgccaaaaag cgtgtcatcc ggattctttt ctacaggaag
    11161 attttcacca gtaagcatac tttcatctat cgcactgctt cctgaaataa ttcgaccatc
    11221 tgctggaatc tgctcaccag gacggatgac tagttgatca ccaacgacta cttcttcaat
    11281 cgctaacgtt tcttctttcc cgtttcttat cacttgggcg gtttttgttt gaagagacat
    11341 caattgtttg attgcattac ctgttttcgt ttttgctgta tgttccaaat atttgccgag
    11401 caaaatcagc gtgatgatca tactgctgct ttcaaaataa agatcttggg ggtgtccacg
    11461 gaaaaatccg ttgtaaatgc tcaatgcaaa ggcagccgat gttccaattg ctaccaatac
    11521 atccatattt ggtgctttcg tcttgattgc atggtacgca cctttataaa aacgagcacc
    11581 gacataaaat tggacaggag cagacagcac taactggacg attggaaaat gaaaaaagtg
    11641 aacaatcgcc gcatggcttc ctaacatcat agcaatcatt gacaacatca acggcaaagt
    11701 caaaaccgta ctcaatataa gatcgaatag catttttttc aaatacgctt gcttttcttc
    11761 tgcaattttt tgtttgtggg cttcatcgta caaaattgca ccataaccga tgttttctac
    11821 gctttggatt aatttttcag tggtcgtccc ttcatatttg acacttgctt tttcagtggc
    11881 caagttgact gtggcattga ccacaccagg ttgtccgttc agctcttttt cgatcctagc
    11941 agaacaattt gcacaagtca tacctgtaat gacgaacgta tccattttgg tttctgctgt
    12001 tgccatcaaa tcacctctgc tttataaccc aatccattga ttgcttgaca aatcttttcg
    12061 gattgaactt ttgtttcatc aaacttgacc gtaccgtttg cttttttcaa atttactttc
    12121 actttttgca cgccgtcaag tgcgcttact gtttcttcta ctcttgctgc acaatgattg
    12181 cagctcatac cttcgattga aaattgttgt ttcatttttt cttcctccta ttgatgttgt
    12241 ttacattcac attgacctgg gatacagtta cattcgatcg tatctactgg ttgcttttgt
    12301 tccagttgtt tcaaaatctc attgatatct tcttgagtca aagtggcttc ttctactaga
    12361 tccgcaatcg tctctccaat cctttttgca cagatatggg aaaaaagatt ttccgtcgca
    12421 ctgcgaactg tttccatctc cgagacagct ggatgataaa tgaatttttt tccttcttgt
    12481 tctgtccaga tcacttcttt ttttaccaaa cgtcccagca aagttttgat cgttgctggt
    12541 ttccaatcca tcgaatcagc taagatctgc gtgatttctt gggctgtcgt ctgtccaagt
    12601 gtccaaataa cacgcatgat ctcccactct gaatcactga tctttactgg cgttttgact
    12661 ttttccattt atttcacacc tttcgtttac aaatgtaatc attagcttat gaatacagat
    12721 tacgcttgta aacaaaaact gtcaattatt tttgctctct attttcaatt tagttaaaaa
    12781 ttcttgtttc tctcgtcttg ttttaaggat gatcagttta tttcttgcat tagctacttg
    12841 gatcagctgc tgtattttcg gttcattgtt ttcttcaaaa tgccagataa aagataaaaa
    12901 ttctaagtat tccctgttta gtttctctga aaatgattca ggcatatctg gccgagattt
    12961 tttgtttatc tttcttttta gagaacgcca aactactcgg tagatggcga gataacgcgg
    13021 cactttaagc caaatgatct tatctgcttc tttaatcctt tcctctaatg tagaagtata
    13081 atttccttca acaatccatg acggattaga taccataaac gattgctgtt cttgcaaaaa
    13141 ccactttttt gcctgaatac tgtagtctgt tgtatgccat aaaaggtcta gcggcagtag
    13201 cggaaagtcg gtgatttctt tgatcttcct tgcgagggtc gattttcccg acccactttg
    13261 tccaatcacg atgatcttca ttcttctccc tcacttctct ttggaatttg gttcgatcat
    13321 accacttata acgccgcaaa aaaacaaggg actgtgacaa aagtcgtgtc aaagtccctt
    13381 gtttcgaata aatggtgttc aatcagctga ccttcagcat taactcaaaa aacggcaaaa
    13441 tatgaggagc tattttttcg tttgttcctc taatactcag gtcaaatcgc tgattttaca
    13501 acctcttcct taaacggtgt tctcctgcca acacctcggt cacaaggcac aatagtcaac
    13561 aaacatgaga agttgttttt tgaaattgct ccttgtgact cggagttaaa cggcaggctc
    13621 actacctctt tcttaaacgg tgttcaaaag cttgcatctg cggtaataag cccagcaaaa
    13681 cgaaaaatat gaggggctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt
    13741 tttcacaacc tctgattcac ggtgttccag aagtagcttc tgcgaaaaac aagccgaaac
    13801 ttcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    13861 ttctgtcaca acctcttgta acaagtattt ctattttaag gctgaaccac tgtttctggt
    13921 tcagtgattt ctttgttttt gtctcgacga gcaattgctg gcaagatcat acctaataag
    13981 atcaagacaa ttggtgtgat gatattcgtg aataattgga accaccatgc tttaggatca
    14041 gcagtataat ctacttttgg caccattcct aggatacatg caaacgcagt gaaggcaaag
    14101 caccaagcac caaagataaa accgattttt ggatttttaa caaatttata ttcagaattg
    14161 aatttcttat aagctttatt caacatcata tatgccaaga acacccatag gtaacgcatt
    14221 ggcatgacaa ctgagttcaa gttcgtcaac cattttacta attcattcat atctttgata
    14281 ccaaagatcg gcaacaaaat aatgattcct actagtatac ctgtcagaag ataaccattc
    14341 accaatgtac ctttacgatt acgtttacgt agccagtttg gtacaaattc tggatccgca
    14401 tctgctaaca agatttttag tggtgcatca attgaaaagg ctaatgcgga gatttgtcca
    14461 atcatgtttg tcaatgcgta aatgatcatc aatgagttac cgataccata gtaattacct
    14521 aaaatttgaa aagcactgta tgccccattt gccatcaagt ctttaggaat attatcgcta
    14581 gcaaacagca tacccattgc gactgagcct aatacagcac aaatccctac cattgctgct
    14641 aagaagatca tccctttagg aaattccttt gcaggatttc ttgtagagtt gacataaggg
    14701 gatatttttt ccgctcctcc aactgcaaat actagcatcg aaacggttgt gaaataactg
    14761 aaatcaaatg ttggaatata tgttttcact tgattcatat gtggtgtggc aaattccatc
    14821 gttggattga tgaacggtgc cgttacagct aaaagaataa ataagatcga catgacgaac
    14881 atcgctgttc cggccagtcc gccaatcact ttcaaagtag aaagtccttt tgttgataaa
    14941 tataaaaagg caagaaatat aataagtgaa ataactgcta ccatttgtac tgacatcgta
    15001 tttacgatat ttccatttcc ttggactgcc caaccgccag caatcaggat tgcttgaggc
    15061 ttttgtgcca aataaggaat atggacgacc cagtaagtcc aagcggcata gtatgccagt
    15121 cgtttattag atgtcgtatt ttcgacccat gaactgacgc ctcctttact atccttgaag
    15181 gctgacccta attgtccaac gatcaatgcg tatggaataa aataaatggc caaaatcaat
    15241 agccaagagg tgacaactga tactccttgc tgagcatagt tattaactac attccccatg
    15301 ccccaaacca tgttaaatgc aattaacgca acagtaaacc agcgtaattg tttgttgttc
    15361 attcgttctc tcctttatga taaaaatttg ttatccagat gtaatcatat tcaattttta
    15421 acaaacttac aagacagtgt tattttatgt gtgtattctt ttatttaata cacatcgttt
    15481 tttcatattt tttccatcaa aaaaataagt tattttcttt tatttcattt accttattga
    15541 atttcaagct attttgaatt ttctgcaaaa ttaaaaacat tagttatttt ataatcctat
    15601 ctattttctt tacatttttc attaagaaaa ggatataaaa aaaagaggac gttcattcgt
    15661 cctcttcatt gatcaacgtt tattttttac atgcaagctt cgcatggcat tcaaaacagc
    15721 taatactgtc accccaacat ctgcaaatac tgcatctccc attgatgcaa aaccaaaagc
    15781 acctaaggca agtaccaaaa tttttatccc aatagcaaag acaatgttct gtttgacgat
    15841 tttcaatgtt ttctttgcta aatggattgc ttctgcaatc ttcgatggtt gatcattcat
    15901 gatgactaca tctgctgctt caattgcagc atcgcttccc aatccaccca ttgcgattcc
    15961 tacatctgct cttgctagca ccggtgcatc gttgattcca tctccgacaa atgcggtctt
    16021 tttattgttt ctttgtaaga tttcctctaa ccgctgcact ttatcttctg gaagcaattc
    16081 gctatatatc ttgtccacgc ctacttgctt cccgatatga tcagcaattt ttttcgaatc
    16141 gcccgtcaac atgactgtgt tcttcacacc agcctttttt aattgaacca atgcgtctac
    16201 ggcatcttct ttcaaggtat ctgcaataac aagataacca ctgtaacttt gatcgatcgc
    16261 tagataaagc aatgtaccga tttcttgact tgaaggaaaa gagatttcaa atctttccat
    16321 caatttgtga ttacctgcta atacttgttt gccatcaact tcagctaaga tcccttcacc
    16381 tgcaaattct tggatattag ctgttggtaa aagcggacct gcgtagcctt ctaaaacaga
    16441 ttgagcaatc ggatgtgtag aaaactgttc tgcactcgca acatattgca tgaatatctt
    16501 tggatcgatt tttgtatcga tcgtctggat cgaaaagtca cctttcgtta atgtgccggt
    16561 tttatcgaat accactgtct cagtttgtgc aagcaactcc aagtaatttg aaccttttac
    16621 taatacaccg attttacttg ctcctccgat accgccaaag aaactaaggg gtacggagat
    16681 cactaatgca catggacatg agatcactaa aaatgtcaat gctcgataga tccattcaga
    16741 aaacgccgct ccagaaacaa caagtggagg taaaacagct aataaaacgg ccaatcctac
    16801 aacgactggt gtgtaatagc gcgcgaattt agtaatgaaa ttttcagctg gtgctttttt
    16861 actgctggca ttttctacta attctaataa tttgctgata gtagaatttt cataagattt
    16921 ttctacttga actaaaaatg gtttattttt attgataaat ccacttaata ctgtttcacc
    16981 aacatataca gatttaggga ctgattctcc tgtaagtgct gacgtatcga ctaatgattc
    17041 tccttcgatg atcgttccat ctaacggtac acgttcacca ggcttcacaa gtacaaactg
    17101 accgatctgc acgtcttcag ggttcacggt catcaatcct tccgtcgtct gcacgtgagc
    17161 tgtttctgga cggatcgcca tcaattcgcg aatcgatttt cttgaatgac tgactgccaa
    17221 tccttggaaa tactcgccga tttgataaaa tagcatcact gctacggctt ctggatattc
    17281 accgatcaac atagcgccaa tcgtagcaac agacataaga aaattctcat caaagatttt
    17341 tcctttgacg atattcatca ctgccttctt cactacatca tatcctatca gcagataaat
    17401 caataaatac cctatcaagc gaattggttc attaaaatca aataatagca ataaagcgat
    17461 acctgcaagg gaaaataaaa tccggaacag cgtatttctc tctttttcat ttccatgttc
    17521 agatactttt tccttatcta tttctgttac tttgacatct ggttctaatt ccttcactac
    17581 tttttttatt tcttgttcaa tcgtagccaa atgatcttcc tctgcgtcaa tcgttagttt
    17641 tccagaagtg aagtttaccg ttgcttcctt gactccattg atcttctgaa ctcctttttc
    17701 gattttcatt gcacagttcg cacaatctaa accttctaat ctgtaacttt tcattttaat
    17761 gacctcctcg cctcaacata tgaatatctc ttcataacta attataaatg aacggttatt
    17821 catatgtcaa ggtatccgaa agaaaaaatg aaaaacatct gcatcggaaa atttcttcca
    17881 tacagatgtt ttggcttatt ctttcttttt cttttcgatc attgccactg ctcttatcca
    17941 acccgcactt gccttcttga tcttgacagg atagcaacta agtttgaagc ctgttgctgg
    18001 caatttgtct aaattcatta atttttcgat aggacaatat cttttttcaa tccctgcaaa
    18061 atgtccttcc cagatattcg aagctccact acgctttcaa aaatatagaa agagttcttt
    18121 aatgaaaatt atttcaactt tccataaaca gtaaccccat ataaataaaa gacgtaaaag
    18181 tcacatggaa gaacttatac aggagaacgc atagagtcag gtgaaataca tgtatcccac
    18241 ctgactctat gctttatcta acctttatta ttctctttta tacaatttct gcaccgaatg
    18301 gcatcaacgc caatcgagca agtttgaaag attgcttggc aaaaggtatc cctatgatcg
    18361 tgatacatag aaggatgccg ctgacgacat gtgcgagagc aattggtagg cctgaaacga
    18421 tcagccagat gatattaaca atcaatgaca tgccagactg gtgataaatt acttctttgc
    18481 caaaaggcgc aaaagaaagg gaggcgatct taaaacattg cataccgatt gggataccta
    18541 tgatcgtgat acaccaaagt atccccgctg ctaaccagct aagaccaccg attagtccgc
    18601 cgaatataaa ccagatgaca tttcctaaac aactcattgt atttctcctt ctattactgt
    18661 ttttgattaa aactgggcaa aaaatgattt tattcttctt tttttagctt ccagtatcaa
    18721 atattctgca aatttttttt ggatttcttc cgtattttct tttgttacta atgtgtgttg
    18781 gattagtttt cctgatggtc gctgatccaa tcgttcttct gactgttgca cgtccgcttg
    18841 cgcaaatccg ataatccgtt tttcttttgt tacagcaacg agcaatagtt tattcttatc
    18901 atttaggtca gctgccgttt ttgcttttac gaatgattct ggatgatcag taatcagctg
    18961 attgatttga ggtagatctt cttttactgc ccaccgtatt tccatcattt ctcacttttc
    19021 tttcgctcat ttttatctcc actaaaaaaa tcgactgcgg caattgcatc tatccagctt
    19081 tgtgcaatga tctcatgtcc gccaatcgtt gggtggaccc catcttctct tgtatatttc
    19141 gaaaaaccat aaacaatccc taaagcattg aatataccat ctaaaggaac cagcaatgcg
    19201 ccatattcct tagcaagttt acgaacaatt tggattttag ggtctaagtc tttacgccag
    19261 ccatgacggt cgtcggttat aggaagtaca aacggctcca tcaagatgat gttgcagatt
    19321 cccactgttt gaagttcatt tagtaatttt cgataaacgg attcaaattg ctgagcagat
    19381 ttctcagttc cgaatatagc ctgatcgcta gtattatgcc aggtatcatt gattccgatc
    19441 aaaatcgata catagtcagg ttgtaaagct aaacaatctt cttgccagcg tacttgcaga
    19501 tctgcaattt tgtctcctcc tatcccacgg ttcatgcatt caaattccat cgttggatat
    19561 tgggagatta gttttccggc aatcaaaagt ggataacctt tacctagatc agttgcttct
    19621 gctcggtctc ttcctgcgtc tgtaatgcta tcaccgataa ataatatacg ttttttcatt
    19681 tgtcaaaaac tcctttttga ataaaacgat gattttcttc cttatattat cccataagcc
    19741 ctatcaagac gaaagagaaa atatatgtat aaaaaatgac accagcctag tcccttcaaa
    19801 caattgaatg aacgctcatc aagtgtctag ttttttatgc tatttttttc ttaacgtgcc
    19861 ggattcccct tgattccttc gttgtttggt ccagagagat cgatcacacc taagcttccg
    19921 tcaatcagac cactgatgac gccatcagaa catatattta taggctcatc taagacttct
    19981 agtaaatgtc cctttactaa tatctcttct agattgcttg ggatagattg gtcataactt
    20041 tatctggtcc gatgatttca agggtgaatt gaacttacat atggattatt gcatgctgaa
    20101 aatacggacc tactccatct tcgaatgtta ctattagttt gttttcaggt gctagatgct
    20161 gctttatttc aacagcagaa gcatcatcaa atcagatttc catttgaatt accttctttg
    20221 taaatgtttg attgattgcg atttcgttat agaaccttgt gaatcaaata gcaatttctt
    20281 ttgctttctt ctcttgtctg ctggaaactt cctttatccc gtattttaaa aaaagaactg
    20341 cgagagttcg cagttctttt ttagaataaa cggtgctctc cggccaactc ctcggtcaca
    20401 agtcacaata gtcatcaaac atgaaaaact gttttttgaa attactcctt gtgactcgga
    20461 gctgaacggc aggcgcacaa cctcttcctt aaacggtgtt caaaagccag tttctcggta
    20521 ttaagccaaa tttatgcaaa aatgtggaaa gccatttcca caaaattttt cttaatactc
    20581 gaaactaaac agctttttca caacctctcc actaaacggt attcaaaagc ttacatctgc
    20641 ggtaataagt cccgccaatt caaaaacatg aacagctgtt tttgcttggc tgttctaatt
    20701 acttgatgca gaacagcttt ttcatcacct cttatggata aattcggttc aacaaacgtg
    20761 ggaatggaat agcttcacgt acatggtcga taccagcaat ccaagtgact gttcgttcaa
    20821 gacctagacc aaaaccagag tgcggaacag aaccgtattt acgtagatca agataccatg
    20881 aatattcttt ttcatccaaa ccgtgattac ggatttgttc tagcaagaaa tcatagtctg
    20941 ttgcccgttc gcttccgcca ataatttcac cgtaaccttc tggtgcgatc atatctgcac
    21001 agatcaccac gtcttctctt gttggatgtg gtttcatgta gaatggcttg atggcttttg
    21061 gatagtttag gataaagaca ggctgttcga atgaattcgc aatgaacgtt tcatgaggtg
    21121 aaccaaagtc ttcgccccat tcgatgtcat caaatccatt tttcttcagt aattcgatcg
    21181 catcatcata agtaatacga gggaatggca attttgtata agtttttaag acttctttgt
    21241 ctcgtcctag tacatctaat gcatattcgc aatgatctaa aacattttgt actaagaatg
    21301 ctacatattg ttcctgtact tctaaacttt cttcttgatg agtgaaagcc atttctggtt
    21361 cgatcatcca gaattcgatc aaatgacgtc gtgtttttga tttttcagca cggaaagtcg
    21421 gaccgaatga aaagactttt ccgaaagcca tagcagcagc ttccatgtat aattggccac
    21481 tttgagataa ataagctttt tgatcaaagt aatttgtttc gaatagatcc gtagtgcctt
    21541 ctggtgcaga accagtcaag atcggcggat caattttgac aaaatgatgt tgattaaaga
    21601 attcatacgt tgcacgaatg atttcatttc tgatctgcat aatcgcatgt tgtcttgaag
    21661 aacgcagcca taaatgacga tgatccatca agaagtctgt tccgtgttct tttggtgtga
    21721 tcggataatc gtggctctcg cttaccactt ccacatttgt aattccgatt tcataaccaa
    21781 attttgaacg actgtcttca cggatttctc cagttactaa cacggatgtt tcttggtgta
    21841 gatttttagc tgtttggaaa acttcttccg acacttcgct ttttacaact acaccttgga
    21901 aaaaggctgt tccgtctctt aattgtaaaa atgcgatttt tccgcttgat cgcttatttg
    21961 cgacccaagc gccgattgtt actgtttctc ccacatggtt tttcgaatcg atgatttgaa
    22021 tttgttccac gtatgactct ccttttatat tccttgaatt tctttaggat taattttgac
    22081 ttttactctc aataaattgc ttgattctgc gtaccgcttc ttctaacgta gcgcgatcag
    22141 tagcataact catgcggaca ttttctggtg ccccaaagcc ttcgccagtc acaagcgcta
    22201 cccctgtttc ttctaaaaga tcttctaccc attgtgtgac attttcatat ttacacatgc
    22261 gtagcgtttc ttttacatta ggaaaaagat aaaaagctcc ttgtggcttt tcaagagaaa
    22321 cacccggcag ttctgccaca agcgaatata acgtattcag gcgttcttca aaggcgttcc
    22381 gcatttcttc cactgtatct tgctcgcctt tcaatgcttc aacagctgca tattgactga
    22441 ctgctgtcgg attactggtt gactgggaag caacagcgat cattccattg atgatcacag
    22501 gatttcccac cgcataacca atgcgccagc ctgtcatcgc atatgtttta gacacgccgt
    22561 tgatgatgat agtctgattt ttgattgctt ctgaaatcgt tgcaattgga gtaaactcat
    22621 tgccattata aactaaacga ccataaatat catcagcaac tatcaaaata tcatttttta
    22681 ctgcccattc accgattgcc tgcagttcat tcttgctata aatcattcca gtcggattcg
    22741 atggagaatt gatgattacg gcttttgttt tttctgaacg tgcttgttct agctgttcaa
    22801 cagtaacttt ccagctattg gattcttccc ctttcacaaa gacagaaagt ccttctgcca
    22861 gttttacttg ctcaccgtaa ctgacccagt aaggcacagg aatgatgact tcatcttgcg
    22921 ggtcaaggat cgtttggaaa agggtataca aagcaaattt cgctccgtct gttacgactg
    22981 tttgtgaagg ttcatagaca agtccatagt ttttcttcag ataatcaacg attgcctgtc
    23041 gaagctctgg gattcctgct gttggtgtat aatagctagc tttcccgttg cggattgctt
    23101 cgattgctgc ttcttggatg ttttctggtg tcgcaaaatc cggttctccc acagtcaaac
    23161 tcagcacatc tctgcctttt gcttttaatg ccttggcctt tgccgcagct gccaaagtga
    23221 cagacggttc taatcgtttt gttctttttg ataactccat cccattcatt cctttacctt
    23281 ttaaattccc ttgattgtct tgacttcatc tccagattca aagccaatca ggtaataatt
    23341 cagtttccca tttgtttctt ttcctactac ttcccacaca ggctgatcat catacatccc
    23401 taaagcagct ttttcgattt ggatttcagg atgggccgca gcaattgtct gttttgcttc
    23461 gttttcagtc agtccctttg tttgatccaa tacttttact ttttcaccag atttaggaat
    23521 gataacggcg atttcttttc ctttatcatt tgtacctgtc agtgaaaagt atgtcttctt
    23581 tcgagtaaac caataaaaat gatcggcttc ctcaagattt gcatatttct ttgcgatttc
    23641 tactgcttct ttcttcgcct gcaccgccgg acgactggaa cgcaagtaaa aaatcgagct
    23701 gatcacgatg accgctaata aaaatacgat caagccaagt aacacgcgat taatcgtttt
    23761 ttcctttgtc tgttttttct tcaagggtcg tcgctccttt atcttcattc cttttcatta
    23821 tagcagattt taattatgaa aaaagacagc tcctctaact tccttcttca tttttcttta
    23881 agaattcatg gatgtcctga tagatttcct ccatagctag ctctttgatc ggtacttttt
    23941 tcggtaatgc atggatgatc cgtgcaccat attttgcagt gactagtctt cgatccaaaa
    24001 taagcaatac gccacgatct gtctccgtcc ttatcaagcg acctaatgct tggcgtaact
    24061 tcaaagctgc tttaggtacg gattcctggt aaaaaggatt gatcccctct gattctaaat
    24121 aagcgttcct agctttgacc atcgggcgaa gcggattatc gaagggtaaa cgtgtgacta
    24181 caacgatctg caaagcatcc ccaggcaaat ctacaccttc ccaaaagctg tcagcgccaa
    24241 acaatacgct tttttctgac aaaatgaagc gtttcaaaag cttctcccgg cttccgccaa
    24301 acccttgagc tagtatttct cgtccttttt ctagcataga cagatgcatt ttctgataga
    24361 ccttctgcaa aacatcatga gaagtgaata acacaagtat tggccgatct tcttgttcga
    24421 tcagacctgt aagtgctgtc tctaaataat cgatatatgc ttgattgctg acttgatgga
    24481 cggcaggtgc ctcttttggc acatagatcc ttgcctgttt ctcataatca taaggagagg
    24541 caatcacttt aagaggtgct gcttcaatgc caagccgctt agagagatag tgtcgattag
    24601 ggccgatctt cagcgttcct ccaatataaa gaatacgatc atatcgagga taccaaacag
    24661 tcgacggcaa catagccgct tgaaaatcat ggagatgaaa aatcatctgc tgcgctgtgt
    24721 tcccaggaac gaaccaatgg atcaaatgat ctcgccagtc ttccagccat tgatccatca
    24781 gcaagtactg cttgtctaat tgatcaaata agctatataa acttgctaaa tcgatccgca
    24841 tctggtttgt ccagctttct tggataatct caaaagtcag acgcatttgt gactgtagct
    24901 gctggatctc ctgataaaaa agctgcaggt gatcaatggc tgtcccagat tccagtggca
    24961 gctgatccaa catctctttt gtcatcatga cttcttctgg atactgtccg ttaggttgct
    25021 tgccgctcac taaactcatt aatccttctg ccatacaaat ctgtgcttcg catagcgcag
    25081 aaagttcttc tcgataaatc gataaccagc gtaaggcttc atgttctttc ggtaaatagc
    25141 tttgtattcg gtcaaacagc tgatttggtt cttctaattg ctggatttgt ttatggaaat
    25201 agttaatact taatcgtttg tcatttactt tttcagcgat ttccggcaaa tgatgtactt
    25261 catcgatcaa caaaaatgga ctagctggaa gcaatggttc ttttcgctgt gtttcttgta
    25321 ttaaaaacgc atgattgaca atcaaaaaat tactttgcct gattttcttt tgcaagaaac
    25381 gaacaaagtc ttgttcgtaa aatgactgat ttcttgctaa aaatccaata cctctgtgcg
    25441 cgatatctgc aaagaatgaa tggttcaaat ttgttaaatg taattcatcc aaatcaccgg
    25501 tcttcgtttg cgtcagccaa actaagaccg ccatttgata caaagcatat tgtttttgtt
    25561 ctggcggttc cacaagtgtg cctttaaaac gctgtaaatc aatatagtgg cgatagcttt
    25621 tgataattac aggttgtatg ggctgatcta gcaattgatt cagcaatgga atgtcttttt
    25681 ctatgatctg ctgctgcaaa actagcgaaa cggtactgat gacagcgggt ttttcaggtg
    25741 tagccaaata actcaatggc agcagataac cgattgtttt acccattcct gtagctgctt
    25801 cgatcatcaa atcttttgat tcatcttttg tgaaatggtc gtagaccgca ttcatcagtc
    25861 tgttctgttc ttttcgatac atcagtgttt taccaaacat tttttctttt gcttgttttt
    25921 ttcttggata tgtccgctcg ccataatgga cggatgtgaa tagttccact tcttttttct
    25981 gcaaagctag cccatcgacg atttctaatg agggatcaag tggctctggt ttttctttca
    26041 tttcttcaat cacatgataa atgaaacgac tcgtatccat acctgtgtgt ctacttaatc
    26101 gcgcaatctg ctctaacgtg atgattggca attttctcat ccgttcttcg atcagcaaaa
    26161 gaagttgacc cgtcacttcg gcatcactat ctgcctgatg aggattctca tgaataaatc
    26221 caaggctttc tgacaaatca gctaagcgaa agcttggttc cgtcggcaag aaaatttgtg
    26281 caagttctac tgtatcgatg cctggtatct tcaaactagg aagtccgcat cgcatgagtt
    26341 catggttcaa aaaattataa tcaaaataaa tattatgcgc gacaaatatg gtgtctgcca
    26401 aaagattgta aatcgtttgg gcgacatctt cgaaataagg cgctttttgg acgcgttgat
    26461 tcgtgatccc tgttaaatgc tggatttgtt ttgaaatctt ccgccctgga tgaatatcga
    26521 tcgaaaaacg tgaagtgatc cgacctgatt caatcatgac gcagccaaat tggatgatcc
    26581 gatcttcttt tggattcgtc ccagtcgttt cgatatcaac gactgcataa gttcttggtt
    26641 tcaaaatatc ttcctcttct cctgcctcta atgttctttt gaaattatac tacaaagtgc
    26701 agaaaaatac tctcatcgct ttttaaagaa aatagacaaa aacgcccaga cacgcaaatg
    26761 tctgggcgtt tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg
    26821 tccgcgttgg ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa
    26881 tccgtcgcct tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat
    26941 gaatccaaat cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat
    27001 cctccttgtg tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt
    27061 ttatttgaaa caagctacca cagattaccg aacaaaaact actcctatag tatatcatac
    27121 cgtttacaaa aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat
    27181 tacataaagt gaaaaaccga acttttttta aaataaacga catattgttc agttggtgtt
    27241 tttttgctct gatgttcttc tttaaactaa aaaaattatt ctttctgact acttatcggt
    27301 tctgtttcaa cggtcgaggc tggggaagat tctttagtag gtctgatgtt ctcatggtat
    27361 ttctgatagc ctatgacagt taccagaaga acaaggcaaa ccaatacgat caacgagatt
    27421 agtttttctt ttttgctcat atctatacct cataaataac aggatgatgt ccatcatatt
    27481 tctgagtatg agtaaaagca aaagctgcag ctgtagaatg taagaaatgc cagcaacttt
    27541 cataatcaca ggtcggacag taccaaaagt aaccaaatct tggctgcaaa gaccgttctt
    27601 ctgtttcagg aatcgcagaa tcaagatctg ttgtctgtac ataaacattt ttgtacagac
    27661 gtgtgcctgt aataggagac cgctgtgttt cttcagcaaa aactggctga cttgttaaac
    27721 ttagggatag aaacagcaaa caccataaca gtcttttttt cataaagacc tccttatcga
    27781 taagtttttc ccaattaaaa tcatatctca agaacgcccg ttattcacat tttgttcata
    27841 aatgatgttt tttgtatcat aatcgtcaaa aagaggtcgt gaccacgtca caacct
//
LOCUS       DAYYGS010000032        27668 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000032 DAYYGS010000000
VERSION     DAYYGS010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.032"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(224..1168)
                     /locus_tag="ACHA44_001274"
     CDS             complement(224..1168)
                     /locus_tag="ACHA44_001274"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8904574.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIYRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1464..2408)
                     /locus_tag="ACHA44_001275"
     CDS             complement(1464..2408)
                     /locus_tag="ACHA44_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8904575.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLKIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2629..3843
                     /locus_tag="ACHA44_001276"
     CDS             2629..3843
                     /locus_tag="ACHA44_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8904576.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3865..5880
                     /locus_tag="ACHA44_001277"
     CDS             3865..5880
                     /locus_tag="ACHA44_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HFM8904577.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5963..7336)
                     /locus_tag="ACHA44_001278"
     CDS             complement(5963..7336)
                     /locus_tag="ACHA44_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8904578.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7362..7646)
                     /locus_tag="ACHA44_001279"
     CDS             complement(7362..7646)
                     /locus_tag="ACHA44_001279"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8904579.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7648..9663)
                     /locus_tag="ACHA44_001280"
     CDS             complement(7648..9663)
                     /locus_tag="ACHA44_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8904580.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9842..10147)
                     /locus_tag="ACHA44_001281"
     CDS             complement(9842..10147)
                     /locus_tag="ACHA44_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HFM8904581.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10236..11267)
                     /locus_tag="ACHA44_001282"
     CDS             complement(10236..11267)
                     /locus_tag="ACHA44_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8904582.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11474..12349
                     /locus_tag="ACHA44_001283"
     CDS             11474..12349
                     /locus_tag="ACHA44_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8904583.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12501..13343)
                     /locus_tag="ACHA44_001284"
     CDS             complement(12501..13343)
                     /locus_tag="ACHA44_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8904584.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13435..15312)
                     /locus_tag="ACHA44_001285"
     CDS             complement(13435..15312)
                     /locus_tag="ACHA44_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8904585.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15309..16730)
                     /locus_tag="ACHA44_001286"
     CDS             complement(15309..16730)
                     /locus_tag="ACHA44_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8904586.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16733..20122)
                     /locus_tag="ACHA44_001287"
     CDS             complement(16733..20122)
                     /locus_tag="ACHA44_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8904587.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20346..20714
                     /locus_tag="ACHA44_001288"
     CDS             20346..20714
                     /locus_tag="ACHA44_001288"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8904588.1"
                     /translation="MIRLLEGNYRKMEKIIRILSISDKWMTKKELAKRIGSSESTFVR
                     YIEEIKIRWGTDVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPG
                     KTIEYYCEKLPSAGLLLHVS"
     gene            20711..20770
                     /locus_tag="ACHA44_001289"
                     /pseudo
     CDS             20711..20770
                     /locus_tag="ACHA44_001289"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            20963..21769
                     /locus_tag="ACHA44_001290"
     CDS             20963..21769
                     /locus_tag="ACHA44_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8904589.1"
                     /translation="MYFVVHLMRESQGTPFEWKAGDSYVQLINIDKKDFLILKNLLGK
                     LKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNMSYCSFSSADCKEKNEEYVVQL
                     LTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLYAGFRAELQFYSTLLNQNLIYY
                     LSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAI
                     TEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(21842..22489)
                     /locus_tag="ACHA44_001291"
     CDS             complement(21842..22489)
                     /locus_tag="ACHA44_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904590.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22497..23183)
                     /locus_tag="ACHA44_001292"
     CDS             complement(22497..23183)
                     /locus_tag="ACHA44_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904591.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23189..24262)
                     /locus_tag="ACHA44_001293"
     CDS             complement(23189..24262)
                     /locus_tag="ACHA44_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904592.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24484..25158
                     /locus_tag="ACHA44_001294"
     CDS             24484..25158
                     /locus_tag="ACHA44_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8904593.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25163..26503
                     /locus_tag="ACHA44_001295"
     CDS             25163..26503
                     /locus_tag="ACHA44_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HFM8904594.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26652..27638)
                     /locus_tag="ACHA44_001296"
     CDS             complement(26652..27638)
                     /locus_tag="ACHA44_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HFM8904595.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8063 a         5237 c         5026 g         9342 t
ORIGIN      
        1 ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta ttgtaatcaa
       61 cagattaaat ttcatatgtt catatgtacg gatacatttt cggacggatt aattttaacc
      121 attctattta tttgcttacc tattagatat caataaaaaa ctgcttaaca gctatttcag
      181 ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat acagtgttca
      241 atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct tcgttgattg
      301 ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca cctgtaaaga
      361 tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca gcttgttcgt
      421 tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag taagttgctt
      481 ttttacgata aatgatctca taagcagcat tacggacttt atcagcaatc acatctaatt
      541 catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta ccaccgacag
      601 ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata tagccgtgga
      661 cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga gttgtatcca
      721 atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc caagcgatat
      781 atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat cctgatccca
      841 tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa tctaaacgag
      901 attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca tctttacatt
      961 cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata ccatctaaaa
     1021 ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact aattcattag
     1081 agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct gtaccgacga
     1141 ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata actatttgtt
     1201 actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat tataatagtt
     1261 gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata aatgacaaat
     1321 gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa taaatccaaa
     1381 aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt ttccttcata
     1441 tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg agtacatgtt
     1501 gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat acgattagcg
     1561 atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc gaattttttc
     1621 tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt actaagatag
     1681 accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt aaaatgctta
     1741 tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc tgatgaaaag
     1801 ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata gagatttaac
     1861 gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc aatcaaatgc
     1921 gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc ccctttttct
     1981 tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa atgattcaat
     2041 atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc ctcttgccct
     2101 gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact ttcttccatc
     2161 tctatccttt ctccattaat aatttttaaa catgtaccgc tatacatata attgatttca
     2221 acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact gatatgaata
     2281 tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag atggtaagct
     2341 aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat tgattcttcg
     2401 ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt tttgtaagcg
     2461 gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta tttttggttt
     2521 cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt gagagaataa
     2581 ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat gcttatgaat
     2641 aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc tgctggtgca
     2701 gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt atctgctgtt
     2761 gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat tagtagtttt
     2821 attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat tgcatcgatt
     2881 tgtggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc aagagctgga
     2941 ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc tgccttttat
     3001 cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg atttggtgca
     3061 gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata taccttttta
     3121 gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc tacacctgaa
     3181 acaactgaac ttcaatcgca tggcacagcc ccaacaagac aagtaatcaa aacgaatcct
     3241 aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa tgctgtatcg
     3301 attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa tgcagcgaat
     3361 atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg ctggcttcag
     3421 acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat cggctttgta
     3481 ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct aacaggaatt
     3541 tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc gcccaagtac
     3601 gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac tttcattgca
     3661 ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact tgctgttttc
     3721 gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt agtacacagg
     3781 acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt gacaacaaaa
     3841 taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag atggctttgg
     3901 tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg ggaagaacga
     3961 ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt caagtttaag
     4021 agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg agttgggtat
     4081 gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc agctggtaaa
     4141 gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt tatcgaggga
     4201 gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga atatccagct
     4261 ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta ttatcaaaaa
     4321 gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt cgattttggg
     4381 cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga atctatcact
     4441 ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta ctataagcaa
     4501 ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt gtttatacca
     4561 gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc tctttcgaaa
     4621 caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt atcagaagaa
     4681 gccttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga tcgctggatt
     4741 tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt tgacgaggaa
     4801 atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca gcatatgaat
     4861 actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta tatgatgacg
     4921 aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat gagatacttg
     4981 atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg gatatttatt
     5041 gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt attactgaaa
     5101 gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg agagaacgaa
     5161 aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa cctatttgac
     5221 tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg gagaagaaat
     5281 gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa agaccaacag
     5341 caatcgatct taaagaatgt cttattgaat gacactatca cacagattac tactccatat
     5401 ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat cgtctaccaa
     5461 acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt ctgggaagaa
     5521 tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc ttatggaaaa
     5581 agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta ttttatcggt
     5641 ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct agattcattt
     5701 gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga aaaaacaaaa
     5761 gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa tggtaaaagt
     5821 tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat aaatgcataa
     5881 aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat attgtccaag
     5941 caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt gttctttttc
     6001 cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt ccaactgtca
     6061 aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact agataatcag
     6121 tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg ataaataatg
     6181 gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct cttctaccgc
     6241 cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct actaccccag
     6301 gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt cctacaaaga
     6361 agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg ataggaacat
     6421 ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat gctggaacga
     6481 tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct gtgaatgttc
     6541 cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct ctttcaacaa
     6601 atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag agcatgatcg
     6661 atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc tctgtagaac
     6721 ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg atatttccgg
     6781 tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt tgacaaagag
     6841 caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc atccacaaat
     6901 ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca taaagcattg
     6961 cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa atattcacga
     7021 tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa gcaatcgcca
     7081 ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg aacatcgaag
     7141 caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact aggaatccga
     7201 caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga agaacgagac
     7261 caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac acaaacaaat
     7321 tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt tgacaagatc
     7381 ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg tacgagcttt
     7441 tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa ataatcagcc
     7501 atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc cacatctgct
     7561 ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt tcctaatcca
     7621 ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga tcgctaatat
     7681 ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag aaagtagctt
     7741 tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta aacagatgaa
     7801 caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa gtttcagcaa
     7861 agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg cgatgtgatc
     7921 gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt tgatataact
     7981 ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg cttcttgcca
     8041 gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag atagcatagg
     8101 acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata cttgatagag
     8161 cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg acaacaattc
     8221 ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt gattgatcag
     8281 ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact ttttcttatc
     8341 agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt caacctcgtg
     8401 taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt gctgaagttc
     8461 actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg ctcttatttt
     8521 tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta tagtgaaaaa
     8581 accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa gtaatgcttt
     8641 tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca tcacattagg
     8701 taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa agacattcat
     8761 caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc gatggatgac
     8821 ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc cttcaacgat
     8881 cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg aatagccaag
     8941 gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca gttcagcatc
     9001 ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca aataataagc
     9061 taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg ttttatctag
     9121 caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat acgtgagccc
     9181 aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt ctcttcttaa
     9241 tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca gctgacaatc
     9301 ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc ctttagaaac
     9361 ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat aaaccatcaa
     9421 ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag taatatccaa
     9481 aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg tatcatccac
     9541 atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt cgatgatctc
     9601 tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa ttttgtagtt
     9661 cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa atcgaggggt
     9721 gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt tctatcgaat
     9781 ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg atttcgtccg
     9841 atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct ttgacatctt
     9901 caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt gtttcctctt
     9961 ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca gagtttgtgc
    10021 cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct aaagccactt
    10081 cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca ccgtcataat
    10141 ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata agacagttta
    10201 tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc tgattcttga
    10261 atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc atctttgatg
    10321 actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg gctgtaatca
    10381 ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat ataggtcaat
    10441 acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat gtaaaacatt
    10501 ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt ttttccgtaa
    10561 acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga aattttcata
    10621 tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc taaaagttga
    10681 cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg atctactgta
    10741 tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt attttctggt
    10801 acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc taattttccg
    10861 ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt ttggtaacat
    10921 tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc ctttgacgtt
    10981 tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat ttttccatat
    11041 tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg atcatcccaa
    11101 agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac tggttcagat
    11161 ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa aggcaaatga
    11221 tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag tggtgcctcc
    11281 ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata atagctattc
    11341 tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt aatgttcaaa
    11401 gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta agatagaatt
    11461 tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt ttaccacgac
    11521 agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct acatgacgat
    11581 tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt tatgcaaaaa
    11641 aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag ttcatcaatt
    11701 gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg aagatagcac
    11761 tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa aagcacaatt
    11821 gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg cacagcgaat
    11881 ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga ataaaatggt
    11941 caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt ggaatgcagc
    12001 caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca tccatcatta
    12061 tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga ttaccggaaa
    12121 tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc aggaggaata
    12181 tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg tattaaatac
    12241 gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact tgaaaaacaa
    12301 acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag aaaagatcct
    12361 catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc ataaaaacag
    12421 gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc gaatagatat
    12481 attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg acgaaaaaga
    12541 tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag gaaaaatcgt
    12601 ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata aggaacccgg
    12661 tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt taggagtgtc
    12721 acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc taccgtcttg
    12781 atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa agaatcacct
    12841 aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg atggctggaa
    12901 ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa taatgaggca
    12961 cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac attgatctca
    13021 gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt tttcgcaggt
    13081 tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc taattgctgg
    13141 tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc tttgaccgct
    13201 tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc gtcgctaaca
    13261 aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag cagatctaga
    13321 agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg ataataaaag
    13381 aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa aagactaaat
    13441 ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga ctactcctgc
    13501 accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt tgtgtttatt
    13561 tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt catcgatagt
    13621 aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt cttccagata
    13681 gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg ttgtaaaaac
    13741 cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt cgatcgataa
    13801 atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag caagtgtttg
    13861 gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa cgacatcgac
    13921 tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt tagaaaatcc
    13981 tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca atgtaccgcc
    14041 aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt ctgtttgctc
    14101 agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg cataagcata
    14161 cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga taagtttgaa
    14221 tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga tattgacaga
    14281 aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt gaggtgttgt
    14341 tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt ggctatttgc
    14401 caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat caggagcttt
    14461 tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact ggcctttttc
    14521 aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag taatgaaatg
    14581 tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac tttggatgta
    14641 ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc catttagtgc
    14701 ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac cgactgtatt
    14761 tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag agccatctgc
    14821 ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag ctgacactcc
    14881 gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt ttttaggtaa
    14941 agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg taccaggagt
    15001 caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc gttgcgcata
    15061 aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt ggtattgatc
    15121 gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat cagtcatttt
    15181 ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt ttgcgccgaa
    15241 gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt ttattttttt
    15301 atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat tgcaaagctt
    15361 gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt ttttggaaga
    15421 aatggacggc tattttcttt tttattgact actttcaata tactgtccgc ctctttatcc
    15481 tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat ataaccagaa
    15541 ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat tgaaaaagcg
    15601 ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca cttgtatccg
    15661 ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac tgcgccagct
    15721 aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc tgtatataca
    15781 gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt gttgatttcc
    15841 ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag tttttttcca
    15901 gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg tttcttgtgt
    15961 atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact attaggtaaa
    16021 aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc aaggatattt
    16081 acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa atcatttcta
    16141 ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc atttttagga
    16201 tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag aacaactacc
    16261 atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa aagatacgct
    16321 ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc atcttcgttg
    16381 ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc gttttttgcg
    16441 atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta aaaagaatct
    16501 gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg taataaagct
    16561 ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa aagcaatttg
    16621 tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt aaaagctggt
    16681 ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat atttagctgc
    16741 cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca atcatcccta
    16801 ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact tttgcctgat
    16861 tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca gattcatgat
    16921 cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact ggctctttta
    16981 gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca ggttttaaat
    17041 tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag tccatttctg
    17101 gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct tttttgtgta
    17161 ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca ttatttgtca
    17221 tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg aattcataat
    17281 ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga tatttaccag
    17341 gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg tcaattggct
    17401 gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta agatttatct
    17461 ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca acaaaaggat
    17521 tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca tcaatggtta
    17581 ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat ccatgccctt
    17641 ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg acattttctg
    17701 gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa tctcctgtta
    17761 atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc gttattttca
    17821 agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt tgttgagaat
    17881 cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga ccttgattat
    17941 tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca gataatttgt
    18001 caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg attcgaatat
    18061 atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca aaagtaactt
    18121 gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg atgtgaagac
    18181 tgactccggg agctttagca gatggtatac cgaattcagc taattcattg gtatcaatgt
    18241 ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg aagtcctcat
    18301 tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt cctaaataga
    18361 tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc gttgtaggac
    18421 ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt tctgcgattg
    18481 gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt ttggctaggt
    18541 attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct ttttcatctg
    18601 atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca gctgccggat
    18661 cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg agtgccatcg
    18721 cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg ttttggtcag
    18781 gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc gtattagaaa
    18841 actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact ttataggaaa
    18901 atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt ggaacggata
    18961 gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt gtccgtgaag
    19021 gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt actgcaccgt
    19081 tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt ttgtctgtga
    19141 taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact tcaccgatcc
    19201 gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca agaggcgtga
    19261 tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg ggtgtacttg
    19321 tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct ccatattcga
    19381 tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca tcccattgat
    19441 ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta ggttgccaac
    19501 tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc gtttgattag
    19561 atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg acagaatttt
    19621 cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac acatctgtat
    19681 ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg atcagcttgt
    19741 ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata gtttgtttag
    19801 tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca tagccatctc
    19861 cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct ttttctatct
    19921 tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt ccagactgat
    19981 ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta gtgtctgcta
    20041 ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa ataactttca
    20101 gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt tttgacgtga
    20161 atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt aatatgataa
    20221 ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt ggtttcattt
    20281 atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt gagggagaaa
    20341 aacatatgat caggttgttg gaaggaaact atagaaagat ggaaaaaatc attcgaattc
    20401 tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt ggtagttcag
    20461 aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg gatgtaacga
    20521 ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca gcttatttaa
    20581 atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat gagattattg
    20641 tcaatccggg taaaacgatt gagtattatt gtgaaaaact tccatcagcc ggtcttcttt
    20701 tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa aagtgatcgt
    20761 tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta tctttgttac
    20821 ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg aaaaaaagaa
    20881 gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg aagaaagccc
    20941 atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag aatcccaagg
    21001 aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata tcgataaaaa
    21061 agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct atataaaagt
    21121 agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa aaaatgaaat
    21181 cagtgagcag atacgaaaaa atatgtccta ctgttcgttt tcatccgctg attgcaaaga
    21241 aaaaaacgaa gaatatgttg ttcaattatt aacgcaaatt tattttttct cccgtatttt
    21301 cccatacgat acgactcatt tgacgatccg tgcaacttac tttgcacagc agatgcaaca
    21361 ccgccagata cgtctatatg ctggatttcg tgcagaactt caattttatt ccactctact
    21421 gaatcagaat ctgatttatt atttatctag ctatatcttt tggatgctta tcggtaatcc
    21481 tgaaattcac cattatactt ctgataaaaa aatactgatt atcagcgact taagtcttcg
    21541 acatagtcaa tatattgaag aatatatcag tgatattctt gctgtgcata aaattcactc
    21601 tgagacaact gctattactg aagatcagct gtcgaaatac aatcttctcg agtatgatct
    21661 gatcgttacg aatcaaccca ttttgaatgc ccatgttcct catatattga ttgatgatag
    21721 tgtgtcattc gcaaatgaag aagaactcat ccgcttattc gagttatgaa taatcaaaaa
    21781 acagctatgt ataaaccacc ctgataaaaa tttcaggaca aatacacata gctgtttctt
    21841 tttattgtat tccttcagca atccaacgag atttatagcg aaagtctttt aacaactctt
    21901 cttttgtcca attatttgca atgagatccg tcaaagtgtt cattacgagg gtcatggaaa
    21961 agtataggaa actaactgct tcttccgacg attgaatgga gaaatgattg attcttaata
    22021 attccagcca tttttcgttc attggagaat cagccgatag ttctctacgt ttggttgtct
    22081 gctgatcatt tcgctgggtt aacaagtata acctttgcca atagatactt tcgtgatcta
    22141 agtcgcagca ccactctaaa tatttttcga tccccaaaaa gaaatgttgt ttgttttcgc
    22201 taatgaattt taagatatct tgatggacta tgtgagaact gcttttgatc gtatacaaat
    22261 aagcatcttc caaatcttga aaatatttat aaaaagcacc gcgcgacatc cccatcttgt
    22321 caacgatttc tgagactttg acttggctga tgtgctgtga gcaaaatatc tccagtaata
    22381 ggtcatcgat tctctgtttc tttgcgttcg ataaattgaa aaaagtttct tttggcatcc
    22441 acatctctcc tttatctctg attatacgtg gatagccctt cattgtcatg aaggattcat
    22501 tccttctttt tctcttcttc tgtcaagtac ccatcttcca ttcgataaac gcgatcactc
    22561 cattgaatca tacggacatc atgagtaacc ataatagtcg ctttttgttt ctcatgagct
    22621 tctttaacta gtaatttgac gacttcatat gcatgatctg tatccaaact agctgttggt
    22681 tcgtctgcta agatcaagct tggctcatta tataaagcgc gagcaattgc cactctctgt
    22741 ctttctccac cagaaaggtc tttaggataa ctatttttaa gtgactggat atctaaagat
    22801 tgcagcaagg aatcgatttt tgttgaatct tctttttttg aagtaacttg gtcaactagt
    22861 tcaaattgtt cctttaccgt taaaaaagga atcaaattag agctttggag aataaatcct
    22921 atttctttaa agcgtaaatc tgctcgtttt ttttctggaa gctcagttag tgagtgaccg
    22981 ttgatcacta tttctccgga agtcggtgtt tgcaatcctc ctgaaatggt aaggaaagta
    23041 cttttacctg aaccagaagg accaatgatg ctgacgaatt ctccttcctt tacataaaaa
    23101 tcaattgctt tcaaagctga gacctttgta tgaccagaac caaatgtttt ggtaactttt
    23161 ttcatcgtta atattttttt catttttatt aacctcctat tgctgtaatt gggtctactt
    23221 tggttatcgt acgtatcgaa aacaatccgc ctagcattgc gacaatgatc aaaatcccac
    23281 tatatagcaa ccattgtgac caaaaaacag caaaaggcat cgcgcttggt aaaatcagac
    23341 tagtcaaata agcaaacaaa agagcaagca gaacgcctac caccccaaca ataaaggact
    23401 gtgctactaa agatttagcg atgaagaaat ttttgatccc ttgtgctttc attactccaa
    23461 aaatagcagt cttctgaagt gtcatcacgt acatgaaaat accgaccacg gcagcaacca
    23521 ctaagaacaa aaagtatatc atcgcattta aggtcatatt ttgggcagaa tagccaggaa
    23581 tactttcaat gaatgtccca atagttaata actgtagacc cttcgatgct ttttctttcg
    23641 aaattttagc tgaatccttt attacgatcc cattgatcgg ctgttcttct tcagaagcaa
    23701 acggctgttg tccatacttc aaagcagtcc acgtatctag ggacgcatag atgacgggag
    23761 aaactgtgta ataagtttca ggaaagatac cgacaatcgt caaaggatcg tcgtaactgc
    23821 caattttgat cgtatctcca attttatagc ctgaatcagc cagattttga gagatgatga
    23881 tttcattctt tgttttaaat tcatgccctt tcgtgagttt tggtaataaa aatgcttctt
    23941 tcgttgtgcc gaaaactgta acattttctt tgttttttcc tttgattgct ccgctgtaca
    24001 atccgattgg tgctttctct cctccttcaa tgtatttcag atctcctctt gttaattgtg
    24061 aagctgcaaa tacttgattt gcatcctccg acaaaacaat ctcttgtgcg tcccaatcat
    24121 cgattgcttt tttaaactct tctgaaagtc cattcgctag tcctgacaac atgaatacga
    24181 catacgcaat caagaacata ataccgacga tcaagccata tcgtaattta gaatagcgaa
    24241 tttctttgat agctagaaac atttgttttt cctccctatg atagattgat agtgacaaaa
    24301 tgtcacttgt tgaatagtgt aactctaaag tgacaatttg tcacttgtgt tgtttttttc
    24361 ttatcaataa attgtctaag acatctttcg ataaaacctt aacatgagtc ataaaaaatt
    24421 tatttaagcc ttatttatgc tttccatgat aaactaaagt gaccaaatca atgaggtggg
    24481 aaaatgatta aactattagt agtagaagac gataaagtat tatccaataa tataaaagaa
    24541 atactcgctg caatcggcga gataacacaa gtttatgatg gtagtgaagg actgttcgag
    24601 ttggaaagtg gagtttatga tcttgcaatc ttggatttga tgatgccgga aatgaatgga
    24661 tatgaagtgt tagcaaaaat acggaaagaa aaaatacaga ctccagtatt gattttgact
    24721 gcaaaagata gtttggaaga taagattgaa ggatttgaac gaggtgcaga tgattatcta
    24781 acaaaacctt tctatcgaga agaactgatc atgcgcgtga aagcattatt gaaacgctcg
    24841 ctaggtctct ttgatgaaaa tgttctgaag tataaggata tcagttgtga tttaaaaagc
    24901 aatgaagtct tgtataaaga acaagttttg cccatacaag gaaaagagtt tgaattgctc
    24961 gtttattttt tacaaaataa gggaatcatt ttgacaaaag agcagatatt tgaccggatc
    25021 tggggatttg attctgatac gacgattaca gttattgaag tttatatgag caacttacga
    25081 aaacatctgc gaagtactgg attggataaa gaaatcaaaa ccttacgtaa tgtcggttat
    25141 atactgcaaa gggtgtgatg caatgaatgg agatattaca cgaaagcaac gtagtcgctt
    25201 ttttgcgaca aatgtagcag catttgcttt tatctttttt cttttgggat tcattacttt
    25261 acagatatta aaccaatctg catacagaga aacagatctg tcattagaga aaataaattt
    25321 tgattctcga acagtacaat tagagataca aagatatcag caaggcgatc cctttttaaa
    25381 cccgactcaa acggaacaaa acagaacaaa catggaacag ccattaatgg aaccatcaaa
    25441 tgaccgtttt aacactcaag tgatcttgtg gtctaaaaat ggtgagatat tgaataaagc
    25501 agctattggt gggcgtttca accaaatcaa agaattaaaa atggatacga aaaatctgaa
    25561 taccatccaa aatattgaat taaatgaaga cagtggctca tctgccctct ctttccgttc
    25621 gattacgaaa aaagcggaga cgaatcaaga tggcgttgcc tacatccaaa ttttagaaaa
    25681 tacaaatcaa atcaagaatt cattagcgac ttttcgcacg attatgattt tgtgtatgat
    25741 tgttttttgg attctctcta ttggtatcag ttattacttg tctagtctaa gcatgcgccc
    25801 tattattctt tcttggagaa gacaaaaaga atttgttgag aatgcttctc atgaattgcg
    25861 cacaccgtta acgattattc aaaatagttt ggaacatctt tttaccaagc ctaatcatac
    25921 tattattgaa gaatccgaaa gcatcgctca agcattgtca gagacgaggc gcctcactgg
    25981 attgacttcg gatttgctga caatcgctcg gaatgattct gatcagcagc tgctttcaaa
    26041 gcaaatgatc aatacacaag aatatatcga acaacttgtt aaaccatttc aagagatggc
    26101 catcatagat ggaaaagagt ttattctcca taacgatgca acattagaag taccaattga
    26161 tgaaaaaaaa atccatcaag ttttggttat tttgctggat aatgcattaa aatatacgaa
    26221 tccagaagat aagatcattt tggattcatc tgttaaccat aaagaatggc tgattcacgt
    26281 aaagaatact ggcccttcta tcagtgaaga agatcaagca aggatttttg atcgatttta
    26341 tcgggaagat caatctcggg cgaaagagac tggcggatac gggcttggct tggcaattgc
    26401 aaaacaggta atcgaacagc atcatggaca gatttctgta tccaattatc ttccacaagg
    26461 agtcgatttt aaagtccaat tgccgttgaa gcctaaaaaa taatgcttat ccctaaaaaa
    26521 gggctgtgac aaaaatcttg tcacagccct tttttgttgc gaataaacgg tgttccagaa
    26581 gtagcttctt cggaaataag ccgaaatttc tttaagctga ccacttctgt cacaacctct
    26641 ttagcttacg cttacgattt tttattgcgg tcttcaatgt gtacatcacc gcttgtgtca
    26701 atatccaatt cttctcggct aacatcttct gtcacttttt caacatcttc atgttcttct
    26761 ttatgaatcc caacttcttc acgaataact gtatgtttgc ttacatctac ttgctcttct
    26821 ttgattggga tagtaatcgt gtcatcttcc aatttttcgt ctgttccctg tgaagattga
    26881 tctgttactg gtttgcgttc gatgacgatt tcttcacgtt tgaccggtac ttctaccgtt
    26941 tcagtatcgt tgaccacatg tttgtggatg tcgacttctc cagttgtcac atcatgtgta
    27001 tttacatcta atttttcttc ttttagttga attgtatcat catctagatt tggtttgcga
    27061 tcgatgtctt cagtcttgct tgtacccatt gtatcagtct cgccaggtac gtccatcgga
    27121 tcagttcctg gtgtcatatt caatggatcc gttgtatttt gaaagcctgt cgtgccacct
    27181 acgtccattg gatctgttcc agtgccagtt tctaatccag tccccattgt gcttgctggg
    27241 ctaacagtat cttgtgtatc cattgtatct tctgttgttt ctggctgacg atagcctttt
    27301 actgtgatga caaattttcc atcagaaata tctcctctat attgatataa aggatcattt
    27361 tctggtgtag ccgtctcaga atcataggta tcaaaagaaa aagtatcttt tattttttcc
    27421 cataaagaac ggtcttcttc accatccatc cgattttcat ctgtttcaac atctatacca
    27481 gacaatccgt caaatcctag tctagatgct gcttcatcag tagtataaat aacgatatca
    27541 tctcgttcat acccttcgtt acgcaaccgt tcaattactg ataatgcttc ttccctcgta
    27601 ctatagcttc cgatgaccgt taaatttttc tcttccatgt aaattcctcc tagcaaatag
    27661 atttatga
//
LOCUS       DAYYGS010000033        27591 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000033 DAYYGS010000000
VERSION     DAYYGS010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.033"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..638)
                     /locus_tag="ACHA44_001297"
     CDS             complement(93..638)
                     /locus_tag="ACHA44_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8904596.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(650..1342)
                     /locus_tag="ACHA44_001298"
     CDS             complement(650..1342)
                     /locus_tag="ACHA44_001298"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFM8904597.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1361..1645)
                     /gene="macP"
                     /locus_tag="ACHA44_001299"
     CDS             complement(1361..1645)
                     /gene="macP"
                     /locus_tag="ACHA44_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HFM8904598.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1658..2221)
                     /locus_tag="ACHA44_001300"
     CDS             complement(1658..2221)
                     /locus_tag="ACHA44_001300"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFM8904599.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2449..3111
                     /locus_tag="ACHA44_001301"
     CDS             2449..3111
                     /locus_tag="ACHA44_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HFM8904600.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3235..4431
                     /locus_tag="ACHA44_001302"
     CDS             3235..4431
                     /locus_tag="ACHA44_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HFM8904601.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4532..5908)
                     /locus_tag="ACHA44_001303"
     CDS             complement(4532..5908)
                     /locus_tag="ACHA44_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8904602.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5945..6812)
                     /locus_tag="ACHA44_001304"
                     /pseudo
     CDS             complement(5945..6812)
                     /locus_tag="ACHA44_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
     gene            complement(6901..7926)
                     /locus_tag="ACHA44_001305"
     CDS             complement(6901..7926)
                     /locus_tag="ACHA44_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8904603.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFNFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7944..8618)
                     /locus_tag="ACHA44_001306"
     CDS             complement(7944..8618)
                     /locus_tag="ACHA44_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8904604.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8802..9695)
                     /locus_tag="ACHA44_001307"
     CDS             complement(8802..9695)
                     /locus_tag="ACHA44_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HFM8904605.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9769..11466)
                     /locus_tag="ACHA44_001308"
     CDS             complement(9769..11466)
                     /locus_tag="ACHA44_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HFM8904606.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11459..12772)
                     /locus_tag="ACHA44_001309"
     CDS             complement(11459..12772)
                     /locus_tag="ACHA44_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin N-terminal domain-containing protein"
                     /protein_id="HFM8904607.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12772..16713)
                     /locus_tag="ACHA44_001310"
     CDS             complement(12772..16713)
                     /locus_tag="ACHA44_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HFM8904608.1"
                     /translation="MEKLDEPIVPSGQALQRIQRQLFNGILERLGPTATQDPYEYIYP
                     SGEVENEQHRYPKFTTNDYTKVLGNWTQSNERYQGDKGSPYDGENLIDDSDYTNGGAN
                     WRNYDYATNNGSTAGEEDRNVPAQTVQLWGNERNFENSYLDYNGAYIKKWVEPVLPSS
                     TASDLHPEDATTLYNVYLDVIGGEKKEISPIDIVFVLDKSASMSELTAGTNSQTKNAA
                     LIEAVNEMSKDLLSDPSLDIRIGMVNFYHNSTAINNHEQISSDIFPLTNDINRLTGSE
                     NTALNRTPIGGTPLTLGLKNGYETLYKDNGGENRNPEKILIVVGDGTPTFSYAPIQSS
                     YRTSTNGAWSNWTVMEDKIAEDNDVLFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDE
                     VNVHYRYGEVKEGDNKATHWVGDGSSNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQE
                     NPPSIFSIGLGIDGNVSGRQRLDAIGRNVLKNIADLEEDGVTPRYYNANNKNDIVTAL
                     EDISSTFKRTIQQATLYDETGFNVSPFGAGRSADIQFYHLDPGENGMKYQEPEIWDEE
                     RHGQAPPEVVATPTTYVGRENQAYKFSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVN
                     NMAYIQNLEDYENDITTGRMYFPSSSIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQ
                     NPESGEAEPIETSKTTTDGIAIFETKMPILGDETGAVSDIFWIREIAGPPRYQLVDET
                     YQFQIKKYAANEDEMLGHYELVDEYTSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNI
                     EGTEYDELYVKLEMRNEFKPIHMNINKLIEGSNMPINGAEFELYETDGNGLEIGYPVA
                     KGLSGVNEVEEEKGRLTFYSVDEDGKYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAE
                     YKIIESQNPEGFKEPDENDTWILRLYDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWV
                     AFTAYNTFDYREIRVRKLDHEGHTLDGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKR
                     LPGIGYFYSRAVGVEKYVDLNYDQPLHLSIGEYEVSESVAPNGYRLSDQVFQFALNEE
                     GKFIIEDTVIEDETAPLPEGYEIIDGVLQVTLEDELAPIDLELLKIDSMNSRRLEGAE
                     FSIEKQNSAGEYEIIEGGLAPDPNDSSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFI
                     LEISYREEPDIENDRVVPGKEAGTLKVEISYYPNEEAETPDLQQELEYELLETNRIRL
                     QLSVGNDPENPLPATGGSGRLFYILLAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16822..17280)
                     /locus_tag="ACHA44_001311"
     CDS             complement(16822..17280)
                     /locus_tag="ACHA44_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8904609.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17578..18096)
                     /locus_tag="ACHA44_001312"
     CDS             complement(17578..18096)
                     /locus_tag="ACHA44_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8904610.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18098..18715)
                     /locus_tag="ACHA44_001313"
     CDS             complement(18098..18715)
                     /locus_tag="ACHA44_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HFM8904611.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18712..19566)
                     /locus_tag="ACHA44_001314"
     CDS             complement(18712..19566)
                     /locus_tag="ACHA44_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904612.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19563..20474)
                     /locus_tag="ACHA44_001315"
     CDS             complement(19563..20474)
                     /locus_tag="ACHA44_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904613.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20515..21849)
                     /locus_tag="ACHA44_001316"
     CDS             complement(20515..21849)
                     /locus_tag="ACHA44_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8904614.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22057..22887
                     /locus_tag="ACHA44_001317"
     CDS             22057..22887
                     /locus_tag="ACHA44_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HFM8904615.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVDGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23131..23380)
                     /locus_tag="ACHA44_001318"
                     /pseudo
     CDS             complement(23131..23380)
                     /locus_tag="ACHA44_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23571..23765)
                     /locus_tag="ACHA44_001319"
     CDS             complement(23571..23765)
                     /locus_tag="ACHA44_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904616.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24270..24455)
                     /locus_tag="ACHA44_001320"
     CDS             complement(24270..24455)
                     /locus_tag="ACHA44_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904617.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25305..25646)
                     /locus_tag="ACHA44_001321"
     CDS             complement(25305..25646)
                     /locus_tag="ACHA44_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8904618.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25646..25876)
                     /locus_tag="ACHA44_001322"
     CDS             complement(25646..25876)
                     /locus_tag="ACHA44_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8904619.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26163..26501
                     /locus_tag="ACHA44_001323"
     CDS             26163..26501
                     /locus_tag="ACHA44_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904620.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26706..27268)
                     /locus_tag="ACHA44_001324"
                     /pseudo
     CDS             complement(26706..27268)
                     /locus_tag="ACHA44_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - obsolete protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         8197 a         5881 c         4712 g         8801 t
ORIGIN      
        1 acgtataaaa aagagcataa aacaaagtcg tttgttttat gctcttcttt tattattttg
       61 tgctttttat tttatcaaat cactttgttt ttttataaaa tttctgcgcc tagtgtggac
      121 tcaaaatgtc tcaatgccca ttcatgtcca acgggatcga aacttgccac ggaatctttg
      181 aaaatatgga gtttgtatcc tagattatac gcatcgactg ctgtatgcaa cacacagata
      241 tctgtacata cgccggtcaa ataaatatct gtgatctggc gttctcgcaa acggatgtct
      301 agatctgttc cactaaaagc ggaataatgc cgtttgtcta tccaataaac attttcctct
      361 tgctcatagt cttggtataa actttttaaa gagccgtaca attcccgtcc acccgttcct
      421 atcacattat gcggaggaaa taagcggttc tccggatgaa aggaatcttt tggatcatgc
      481 gcatcaatgg caaaaacgac aaaatccttt tgatcaataa acttctttgt atgagcagtc
      541 agcttttttt caattacttg tcctgctgtc ccagtagtca gctttccatc ctctgcaaca
      601 aaatcatatg tgtagtcaat cgagatcaat gcgttcatct tagtcctcct tatacgagat
      661 gtttgacaaa ttcgatcacc atttctgctg atcgtttgcc tgcgtcttcg ataaattcgt
      721 caaagctttg tgtcgccgag tgatctgctg tgtcgctcat tgcacgtacg atcaaaaatg
      781 ggatattgaa ctgacgagct gtttgtccaa cagcggctcc ttccatttcg caagctaatg
      841 cttcagggaa attcgttaag atttctttga ttttatctgg cgagtcgaca aatgtatcac
      901 ctgtaacgat caatccttct tttgcgttca aattcgttgc ttttgctgct ttaaccattt
      961 cactgcgcaa atatgtgctt gcttcataat atagaggcat tcctggtaat tgtcctgggt
     1021 tgtagccaaa tcctgttgca tctgcatcaa aataagctac tttgtctgat ataacgatgt
     1081 cacctacttg taagccttca ccgattccgc cagctgaacc tgtattgatc accatgttca
     1141 cgccgtattg ttggatcaac agacttgtgg taatagatgc taagactttt cctataccgg
     1201 aacgtacgac gatcacttcg tggttaccta atgaacctga aataaataaa gcgcctgcgc
     1261 gttcccatga taatggttcg cttaggttct ctctcaaaat ttttacttct tcttccattg
     1321 cgccgataat accgattttc atctttttaa ttccttcttt ctatataaat gcgattgcta
     1381 aaaaaaccac acataacatt aggataacaa taataagaga tttcatcaaa aaagagttcc
     1441 atttggtcgt cttttcccgt tcgctgatcc ttgttttcgt gattggttta ttctttttgc
     1501 tttctttacg ataaaagctg gcaatctttt tttcttcttg ctgataagct gtttctgctt
     1561 ttcgctgttt ttttaatgac tcgcttgctt gtgcttgttg tcgcttgcga agttcgcttc
     1621 gcgtaataag cggtcctttg gccataagcc gatccctcta ctttcctttg gtcaatagtt
     1681 cccagtattg aatcgcataa atcgtctttg catcacaaat caactgatct ttcatcgctt
     1741 gttgcgcctc ttcaagagtt agatgataca actccagtac ttcgtcttca tcttgtgcta
     1801 aaggattctc gaccttctct accccttgtg catgataaat atgaaggact tcatttgcaa
     1861 aacctggtga aagatacatc gaagtaagat ggctcagcga cttcgcacga tagccagttt
     1921 cttcttctaa ttctctagct gctgtcattt cgggattttg tccttctcct ggatcgattt
     1981 ttccagctgg aatctccaaa atcacttttt ctaaaggttt tcgaaattgt ttgacaagaa
     2041 gcagacgatc ttgttcgtca aaagcaatga tcccgacacc gccaggatga aataccagtt
     2101 ctctcttcga agttcctcca tctggcaaac gaacttcgtc tacagcaaca tcaataattt
     2161 ttccgctata gatttctttg cgactgattg ttttttcttc aaattgctca ttgttcagca
     2221 tatcttctcc tgctttcttt gtaatcataa ctgctttttt ttcatttttc aatttttctc
     2281 aaaaactcct tattagttta ccatagataa accttagatt ttctctaaat atagaaaatc
     2341 cttctttagt ccgatactct ttctagtagt ccgtatgcta aaataagtat gtaatcgaat
     2401 aacgaaaaga attcaatact caacgataaa tgacatggag ggtttattat gataaaacga
     2461 aattggccgt ggcttctagc attggtcttg ctatttctct ttgtccgaaa caatagtttt
     2521 tcttttgttc taggactttt attgataggt gtagttttag ttttgtgggg actctttggt
     2581 tcgcgcagga aaaagggaaa acaagaagaa attcctgcac tgactgacga acttgaaact
     2641 cactatgaaa aaagcggtat gacagcgaat gaaatttcat tttttcgaca aacaatgaat
     2701 caaacaaaaa atgagatcaa tcaattgcaa atgaatatgc agcagacagc caaacttaaa
     2761 gcaatcgatc tccgccatga tccagtcaaa gcagcgaaag ctttattcaa agaattggtg
     2821 aaagaaccaa accgactgca tgaagcaagt cagtttcttt atacgcatct gccgaatcta
     2881 gttgatttga caaataagta taatgagatc aatgaccatg agatcaaaaa caaacagacc
     2941 tatgaaaaac tagaagaaag cgcgcagatc atcgaccagc tgtcagcttt aatcgcacaa
     3001 gattatcaaa aattcgttgc tgaggatcta gatgatatcg acgtagagat atctgttgcg
     3061 aaacagagct taaagcgtga taataaatat gaaccagaac aaaaggatta actcttttgt
     3121 tcataaaaga agactcggaa cattcagatg atgtctggct gtttcgagta ctttttctaa
     3181 agtagagaca ttcatgtaag aatcgagaac agattcaaca aggaggaatt ttttatggat
     3241 cataaaccga ctgaaacaaa agatgttaca ccagttaacg atacacttga agatttattg
     3301 aacaacccgt tttcaactcc aacagatacg ctgaccactt cacaacaagc ggagattgat
     3361 cagctccaag agcaacagac agctgctcgt ttaattgaca aactaccggc ggaacgccaa
     3421 gaacaagcta aacagctagc agcaaaaatc gatgctaatg atgcacagtc tgtcatcagc
     3481 tacggatcgg ctgcccaagc aaaattaagt gaattctcac aatcgatgtt gaatcatgtg
     3541 caagcacaag atattggtcc tgtaggagat tccttaactg aactgatgta tcgtctccaa
     3601 gaagccaatc ctgacgaact gcgtgccgga gaagggaaca tcttccagcg tgtcttcggg
     3661 aaggtcaaac aatcgatcta tgaagtgaca gcaaaatatc aaaagattgg tgcacaaatt
     3721 gacaagattt cagtgaaatt agataaagaa aaagacggtc tgctaaaaga taatctgatg
     3781 ttagaacagc tttaccagaa aaacaaagac tactttgatg cactgaatat ttatatcgct
     3841 gctggagaat tgaaaatgga agaacttcaa acaacgatca ttccagaagc gatgaaaaga
     3901 gcagaagaaa ctggcgatca gatggatgtt cagattgcca atgattacac gcaattccta
     3961 gatcgtctgg ataaacgtac gcatgatctt cgcttagcta gacagatcac gatccagcaa
     4021 gcaccacaaa tccgtttgat ccaaaacaca aatcaggcct tggctgaaaa gatccaagca
     4081 tctatcgcta cagccattcc gctgtggaaa aaccaagtcg tgatcgcatt gaccttgctt
     4141 cgtcaaaaag atgcagtaac tgctcaacgc caagtctctg aaacaacaaa cgacttattg
     4201 aagaaaaatt cagaaatgct gaaaatctca gccatcgaaa cagcgaaaga aaatgaacgt
     4261 ggaatcgtgg atatcgagac ccttcaaacc actcaaaatg atttgatcga aacaatccaa
     4321 gagacattgc gtatccaaaa agaaggaaaa gaaaaacgtc gtcatgcaga agtagagctt
     4381 ggacacatgg aagaagatct gaaacaaaaa ctgttggatt taacccaata gatcaccttt
     4441 cgcttgttac taaaacaaac gcccaacaac agtttattac aactgttgat gggcgttttt
     4501 cttatctttc ttctaacttc tccattggag tctatttctc attcatcttg ctgtgtccat
     4561 agccatagcc aaaataaatg atcagtccaa taaccattac gataccgaac acgatccatg
     4621 tcacagtttg cagacgaagc atcaatacaa tacaagtcaa aatggagacg attggcaata
     4681 gcggaacgaa tggcactttg aattctccag cttttggttt gccaaacatt tttctcaact
     4741 tcagtaagcc tagactaaca agcatcaaat aggcgagcgc catgatattc gttagttccg
     4801 ctaatacatt caatgggaaa aatccggcaa agaatacagc tgccacacca gcggcataag
     4861 tcgctttttt cggtgtacga tgcttttgat cgatttctgt cataaactta ggaagtaagc
     4921 catccttgct gacagcaaac agtaaacgag caagtgaata catcatcgag atcgttaccg
     4981 tcagtagcgt taaaattgcc cctacagcga tcaccgcgcc cactgttcca tgtcctacat
     5041 acctcattgc aaaagccact gggtcattga ctcctagttc agtatacgga acgattcctg
     5101 tcaagatcag cgtcacgatg atgtaaagga cagtagcaat cagtatcgaa ccaatgattc
     5161 ctcttggcac atctttttgc gggtttttga cttcctcggc tgccattgca acggcatcaa
     5221 atcccaagaa agcaaaaaac acaagagctg caccatctag cacaccggaa aaaccaaaag
     5281 gcatgaatgg ctgccagtta tcaggtttaa cgaaaaatat tcctacaatg ataaacaacg
     5341 cgatgatacc aaatttgaca tataccatgg cattattcaa acgtaatgct ttcttggctt
     5401 caagcgacac gatgtaagtg acgaatatca ctacgagcac cgcaatcaag tcaatgtatg
     5461 taccattttc cggattgtac ccgccagtta atgcttgtgg aagttcggcc cctaaacttt
     5521 tcaaaaaacc ttgcatatag ccagaccatc cggaagcaac agaggaaacc gctagtaaaa
     5581 attccccgat caacaaccac cctgtcagcc aggcagcaaa ttcaccaaat acaacataca
     5641 gataggcata tggcccacca atcactggca ctcgcgaagc aaattctgcg aaacttagtc
     5701 cagacaaaat gacaacgatt gcagctaaga caaatgaaag tgacagtgct ggaccagcat
     5761 tttgattggc cgcaacccct gtcaccacaa atattcccgt accgatgatc gcaccaattc
     5821 caagcatgat caaatcagac gtttttagtt cttttttcat ctcactcgta tgattgagat
     5881 tgatctcttt cttacgaaac aaatccattg aagttcctcc ccattttagg ttggaaaaca
     5941 gcgattaaac tgttttcata taccctaaaa gggataccac ctaaaatgac taaaaggcaa
     6001 ccaacaataa cataaatgag ctcttttttc gtttttcttt cgccaaggat gaaaatccca
     6061 cctaaagtag agataatgat ccccatttgt gaaagtgaga agccaacagc taaaccaagt
     6121 gatttgactg ttagaagcat gcagatgttt cctaagcccc ataataatcc acagatcatg
     6181 tttttccaca caaactgatc gactttaact ttacggaaag caaagaagct tgcgcctaag
     6241 atcattccga tactttgagg taagataatt gacatcgcat ccaaattcgc ccagttgacg
     6301 atgatggtat atacaccata accaatcgta gagaagatca atgctctaaa acccttacca
     6361 aaatcaagca ttttattatc tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa
     6421 tacgcaccgc ttacaagaag agctaaagca ataataccgt atacataatc tttcgtttgt
     6481 gtccattcat ggaagaaaac ggctcccgca atcgtattca aaattaattg aaaaccagta
     6541 gacaatggta gccctacaga aacacccatg tacttcattc catggaactg tccattttgt
     6601 ccaacactcc aagctaagcc ggataaaaat ccgatcaaga aaatccaagg ggtgattaca
     6661 ggactaacaa cgaaaaagac aactaaagaa aacagaaatg caccgatcgt cattcctagt
     6721 gtttgttgat tggcatcgcc accaatttta ccacttacta aaccaatact accccatgcc
     6781 aacataggga ctaatgctat taaaatttcc ataaccaatc tccttttatt tcacatgata
     6841 agaaacttat cagcgaaaac gttaacaaca caagcacttt ttttcgtttc taacgatttt
     6901 ctaacgtttt tcattataaa aatttaagat tgggaaaaat aaagtcattg ttgtcccttt
     6961 tccttcagtt gattcagccg tcaaatggat acctaatttt tcggccagat tttttgctaa
     7021 ataaagtcct aatcctgttg aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc
     7081 cttatcaaag atccgtttca agtcttccgc tgggataccg atccctgaat ctttaactga
     7141 aaggctgact ccttgcgctt ccttttctat gctcacaatg atgtttccgt gatccggcgt
     7201 atatttgatg gcattactaa gcaattggtt gaagttaaaa gcgatccatt tagcatcggt
     7261 caaaacagta tgatcctcac caacgatctc gtagcgaata tttttttgga taaaataatt
     7321 tgcctgactg cgtatcacag gctgaatgat tccttttagt ccatattctt ggatgaggta
     7381 gtcccgcgag aaactgtcga gtcttgcata atacaaaact tgttctacgt attcatcgat
     7441 ttttgataat tcattttcta gtaatgtata cttttgatcg tcaatatcga actcgatcga
     7501 acgaactagc aatttagaag cggaaagtgg cactttgatt tcatggaccc atgagtcaat
     7561 ataatctttt tggtcttgtt gattactgat tgctgattgc attacttctt ggtgttcacg
     7621 gatcagctga ttgatatatt cttctaccag tttttcttct tctgtcctcc caccactcaa
     7681 ataattttgc aaaggggaag ttttttgtat gtcggctaat tttccccacc atcttctctt
     7741 gctcaagtaa ccacccagca gataaaaaat cagaaagacg ccctcaatca aagctaaata
     7801 agcaattgtg ctcaaatcaa ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa
     7861 tgcaataaaa aacagccatc ccaagatcaa tgtccactga tctttcaaat atttaaaaaa
     7921 attcatctta accgctcatc ctttcaggga acgatatatc cctgtcctac ttttgtttcg
     7981 atatatcctt ggatacctgc ctgttcgatt tttcggcgta accgattgat gttgactgtc
     8041 aatgtattgt catctacaaa tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga
     8101 cttaagatct ttccattgtg cttcatcaaa ataaataata gacgatattc atttttgctc
     8161 aaatcaatga tctctccgtt gatttccata cttccgttgt caacattcaa cgtaatgcca
     8221 ttgtggctca tcatttcact agataattct gtgtagttgt aagaacggcg aagtaatgca
     8281 ttgatttttg caatcagtac atcgatttgg aaatgttttg tcacaaaatc atctgccccc
     8341 atgttcatag ccatgatcat atccatattc gtattcctgc tggagataaa aatgatcggt
     8401 actttcgata cttcacgtat tttttgacac caataatagc catccaaaac gggaagatta
     8461 atatctagta atacaagctg tggttcttct ctttcaaagt ctgagaacac ttgattgaaa
     8521 tcggtcgttc caaaggtttc aaattgccat ttctcaagtt cctccatgat caattgtcga
     8581 atgatctctt cgtcttcgac taccatgatt tttgccataa aaaatccctc catctttctt
     8641 cttgctacta caatatcaaa gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc
     8701 attttagcaa acgttaaaaa aaactggaaa tatgcgattt aatcgcgttt gtctcttcca
     8761 agcagcttaa atttattatg ttattcttaa taattggtct attatctgtt atttttcttt
     8821 cgttgttttt gtcttttaaa ttttcgataa caccaataac cgaaaagcgt tagcactagc
     8881 aatataccta gaaggagtat aagtaatcga taaagatggt atttttttgt ctgttctatc
     8941 tttgaagaga cttcttctgt aaagggaact cgtcttcctg tcactagtaa tcggtgagtg
     9001 ttcacggtgt aaggtgtaca agtgataagt gtgactagat cctgcttttc tctaattgct
     9061 aatgtatttg tttttgttgg aaggacaaca attttctcaa cgacttcata agcgagcatc
     9121 cgaccataca cttcaagata gaatcggtca tcttgctcca tttcttcaag atccgtaaag
     9181 agttttttct cagcaatacc gctatgggcg gtaatcacgg aatgtgtact ctctcctcca
     9241 gttggaaaag aggtgccttg caggactgtc gctcctctat ctaacaaaag atcattcgtt
     9301 tcatcaaata aaggcaagct aacattgatt ttcggaatat agatggctcc gatcatatgt
     9361 tgttcgtaat attcttttcc tggattttgg acatttctaa ccgcttgctc aaaaggatct
     9421 ttgacttgtc ccattcctgg gattactgat gtatgagcaa gcgtctgatt tttcttttca
     9481 tgcactgcta attgttgctc atgtttttct tctttatcgc gttcaagctg cttttgataa
     9541 ttctcgatcc ttctttgatc caaataattg ctgagagcat ctgctaaaaa tggatatgta
     9601 aaaactgcag ctccggcaag aaacatcact gccataaata attgtaataa aagactgatt
     9661 tgggtttgag gtctattacc tattttcttt ttcattttct tcaccttctt atttaaaaaa
     9721 actgcagcaa cctttataaa accgattgct gcagcttatt tttgttgctt atacttcaga
     9781 cttcatttta gatttacggt accagctgta cgctccaatc atcaaactta ttccgattag
     9841 taagaatgcc aagagcccgt tcccaccagt agcaggaagt gacccgcgtt tcatattcgg
     9901 tactctttct ggccctggat tttctactcc tacgatctga atttcttcat tatagcttcc
     9961 ataagagatg ataaattcag taaaggctgc ctcacctgtt ggaagtacat aaccttctgg
    10021 cgccatggtc tctttcattt gataggttcc ataatctaac ccttgaacct caaagtatcc
    10081 atttccatct gcgacaaatt ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc
    10141 tcctgattca taaacagcta aaggcgactc ccctttataa aatattgcgt attcagaacg
    10201 gtcttcattc cacaattcga atcttgcatc tttaagcaat tcactgcttg atgcatcata
    10261 tttttcaaac tttcttccac ctgttgtgac tttctctggc ggctctactc cttcttcata
    10321 atcaaattca tcatttcgtc cgatttcaat aaccgctgtg ttgttatgga aatcatccgc
    10381 ttctagttct gtggaaacct tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc
    10441 tgctaatcta cctaaagtat ctactttggc tactccattt tcttctgtca aaatcgtcac
    10501 gacaaacccg ttgcttacta cttctactat ataatcttgt ccttttgtta aaatcgtctc
    10561 gccggctcgc acttctattt cctgatcata ataagttaga ccaactgtag gcgtatctgt
    10621 gattttgtaa tttttgataa cagcgcttcc gttttcaagg acagaatcga tgaaataagg
    10681 aataggtgct gtgatcgtgt aagaagccgt ttttcctctt tctagatcaa tataagaaat
    10741 cgtttcttgt tctccagctt cattaataat cgttacttgt ctaaggtcgc tttcctctac
    10801 attcatctgt ttatctgctt ctctgatttc gtttttcgga tacaaatgca catctgtatt
    10861 gtatgtgtct ttccatgtag aaccatccca cacgccatcc accatatcag gcatcatcac
    10921 tggcatcata acaaccattg gagacgcgat tctggaaata tgagctggcg aataagtttc
    10981 taagaaaaga tagactttat ctctagaaga aggtgcttct tccgtcactt ggacggtaga
    11041 gaataccgct tctccagctt cattcgttgt ttgtttgtca atacttgtca gtcgatattt
    11101 taaaaaccaa ctttctgtgt gccgtttttg aatccattca atcgcttcgg cagaattaag
    11161 accgtcttct cgttccggtg ttaaaggatt atctcgaatc aaatcgtaat atacttccgt
    11221 tacgtcaaac aaatcaaaat cgactcctgg aatattttct tcaccaaaat ccgtagaaac
    11281 caatccgcta ttcggtcgtt cttccggaac agatgagaaa ccttttttgt gtaatgtaat
    11341 cgtcacttca cttggttttt gtgcgacttc ttcggctttg actagttttc cgttcccgaa
    11401 aatccctatt cccgcagtaa cgagtaacag tacactcatt cccgtgacaa tcattttttt
    11461 attcattttt ttctccccgt tcttttttta ttaataaaac agcacctaac aataaaagaa
    11521 ttcctagtaa agaaatcaac gttcttgctt cattcgtttg cgggaatacg gaccattttc
    11581 ctgattgacc tggattgctt ggaccactcg gctctttcgg tttgtccgtt gtttctttta
    11641 cggtctcgtt ataaacacgt ggtgttgcgg attcataggc agagactgga actttcccgg
    11701 attctaattc agccatctcc tgtccgttaa ctactacata ttgtggttgg ccttttgcat
    11761 ccacccaaaa ttcagggatg atgacctcga ttctcttact tgcttcatcc atctcgtaac
    11821 cttccacacc ttttaattct tcaaaataat aggtgccaga aggcaagaaa cgttgtcctg
    11881 tcgtaacaag gccctcttta tccgaaatga atttgcttac atttttgtca ttcaaaggat
    11941 cattgtctgc tggtttgatc cattgatttt tcaagtcatt agcaggagcc atatctaaat
    12001 aatatttccc cccttctatc atctgatata gagcaaaaac agcaccttct aatggttttc
    12061 ctttttcagt tgttcctctt tgacgtccat acttgaagaa gtatggatct cttagataac
    12121 cgacattttt aggatagata tgaatctctt gcagttcctc ggttgattct gtcgggtgat
    12181 aaattggcaa aatagcagca attgggatag ctgtctgatc gatatcaaca tccaatccaa
    12241 cttctggatc aatcgattct tcgaaaatca aataaactgc atcccgatct tggtttttct
    12301 taggtgcttt gatacgtgca atcccgtctc ctaatccgcc aaccgtatct ttcccagttg
    12361 ttaccgaagt ccctatttgg acaagccttt cttctgtgat tagctgacgg atattgtttc
    12421 tgttcgtttc agcgatttgc tggacgaatt ctttaggagt catgttttca gcagcttttt
    12481 tttcatgata ataatcggtc atgtcgtaaa tggcaaatgt cgctccattt agtggcaccg
    12541 tctgtgaaat gatatctgct gtttcccctg cttcctcaga gatagccaag ccatcatttt
    12601 catggtattc aaattgatcg ttataatcga tatctcgaat catccgttta tgcaacacga
    12661 attccacgac atcttcccct gattcttctg ccgacatcgc atgtggaaga aggactgctg
    12721 caagcggtag taaaacaagt aattgaagca tcaaacacaa cagctttttc attaccttgc
    12781 tccctccttt ttagattgtc tgacaaacaa accataggct gctcctgcaa gcgccatgaa
    12841 gaatgcagca agcaggatat agaataatcg tccggatcct cctgtagcag gcaacggatt
    12901 ttcaggatcg ttcccgacac taagctgtaa tctgattcga tttgtctcca aaagttcata
    12961 ttctaactct tgctgtagat caggcgtctc tgcttcttcg tttggataat acgaaatctc
    13021 aacttttagt gtacccgctt cttttcctgg aactacccga tcattttcta tgtcaggttc
    13081 ttctcgataa ctaatttcta aaataaagta acctggaagt tttctatagc tgtcaggtga
    13141 cttcacttcc ttgatccgat agaccccttc caacaagtct gaagcattga atagggaaga
    13201 atcgtttgga tctggtgcca agcctccctc gatgatttca tactctcctg cactattttg
    13261 tttctcaatt gaaaattctg cgccttcaag acgtcggcta ttcatactgt caattttcaa
    13321 aagttccaaa tcgattggcg ctagttcatc ttctaaggtt acttgtagta caccatcgat
    13381 gatttcatag ccttccggca agggagcagt ttcgtcttcg atgaccgtat cttcaatgat
    13441 aaacttacct tcttcattca gtgcaaactg gaacacttga tcagatagcc gatacccatt
    13501 tggtgcgaca gattccgaga cctcatattc accaatagac agatgcagag gttggtcgta
    13561 atttaaatcg acatactttt ctactcctac ggcacgagaa tagaagtatc cgatcccagg
    13621 caaacgtttc gattcttctg gttctaacgg attaccagtg tatgctcgat acagcgggaa
    13681 ggaagaatcc cccactttcc tgatatcgaa acctgcacca tctaatgtat ggccttcatg
    13741 gtctaatttt ctaactcgga tctcccgata atcaaacgta ttgtaagcag tgaatgctac
    13801 ccataaacga tcctcttcat ctgttgtata cgtgagatat tgccaatttt cttcacctgt
    13861 ttgcttgagt tggatagtcc ctatagcatt atcgtaaagt cgcaagatcc atgtgtcatt
    13921 ttcatcaggt tctttgaaac cttctgggtt ctggctctca atgattttgt attctgcata
    13981 gttggtagtt tcttcactat ccttataaac aaatgaatct ggctctccga ttggatgaat
    14041 aactttctca ccatccacga ttacatattt cccgtcttca tctactgaat agaacgtgag
    14101 tctccctttc tcttcctcta cttcgttcac accagaaagc cctttcgcta ctggatatcc
    14161 gatttccagt ccgttaccat ctgtttcata aagttcaaac tctgcgccat tgattggcat
    14221 attactgcct tcaattagtt tattgatatt catgtggatt ggcttgaatt catttcgcat
    14281 ttccagtttg acatacaact catcatactc tgtgccttct atgttaggct cttgtacaac
    14341 agaaagcttg tgatattctc cttcataata ggaattcgga tctttacctt ccggtccgcc
    14401 gactctcgaa ccagatgtat attcatctac aagctcgtag tgacctaaca tctcatcttc
    14461 gttggctgca tattttttga tttggaattg atacgtctca tctactaact gataacgtgg
    14521 tggtccagcg atctctctga tccaaaatat gtcgctgact gctccagttt catctcctaa
    14581 aatcggcatc ttcgtttcaa agatcgctat tccgtccgtg gtagttttac ttgtttcgat
    14641 tggttcggct tccccactct ctggattttg gtcatataat gcaaattcga ctccagccaa
    14701 cggctcgcca ttttgaccct tcttgctgac ttggaaatta cggcctttat gttcgtaacg
    14761 aatggaagaa gatgggaaat acatccgtcc agtagtgata tcattctcat aatcctccag
    14821 gttctgaata taggccatat tgttcactgt gcaaaaattt ccgtcttgag ctcccgcatc
    14881 aagttttact tggtacttga tcctgaccat ttccccttca cctaaagaaa tcggagaaaa
    14941 tttataagct tggttttccc gtccgacata cgttgtcgga gtcgccacta cttctggtgg
    15001 ggcttgtcca tgacgttctt catcccatat ttctggctct tggtacttca tgccgttttc
    15061 tcctggatct agatggtaaa actgaatatc tgccgatctg ccagctccaa atggtgagac
    15121 attaaaacca gtttcatcat aaagtgtggc ttgctggatc gttctcttga aggtagaaga
    15181 gatatcctcc aaggctgtaa caatgtcatt cttgttgttc gcattgtaat atcgcggtgt
    15241 tacgccatct tcttctaaat cagcaatatt tttcaataca tttcgtccaa tagcatctag
    15301 tcgttgtctc ccgctaacgt ttccatcgat tcctaaacca atggagaaga tacttggcgg
    15361 gttctcttga tacttgtttt tcagccaatg atgataagca actgtgttga tcgcagatga
    15421 cttttcttgc gaagtaggac tgccattcgt attgttatta gaagaaccat caccaaccca
    15481 gtgagtcgct ttattatctc cttcttttac ctctccataa cgatagtgta catttacttc
    15541 atcttctgga cggttaaagt cagatgcata ggtaactgga tgagtaaacc ctgcattaga
    15601 agtattccca gaaaactcct caaaatttct gaacaaaacg tcattatctt cagcaatctt
    15661 atcttccatc acagtccagt tgctccaagc tccattagtg ctcgtacgat aacttgactg
    15721 aatcggtgca taactgaagg tcggtgtccc atctcccaca acgattagga ttttttctgg
    15781 atttctattt tctccgccat tgtctttata caatgtttca tagccatttt taagccctag
    15841 tgtaagcgga gttcctccga ttggtgtacg atttaatgct gtattctcac ttcccgtcaa
    15901 acgattgata tcatttgtca atggaaagat gtccgaactg atttgctcat ggttattgat
    15961 agctgtacta ttatggtaaa aattcaccat accgattcga atatccagac tcgggtcaga
    16021 gagcagatct ttactcattt cgtttactgc ctcaatcaac gcagcatttt tagtttgact
    16081 attggtccca gcggttaatt cactcatact cgctgactta tctagaacaa aaacgatatc
    16141 gattggactg atttcctttt tctcgccccc gatgacatct agatagacat tgtaaagggt
    16201 tgttgcatct tcaggatgta gatcagatgc tgtgctgctt ggcagaacag gttctaccca
    16261 ttttttgatg tacgccccgt tataatccaa gtaactgttt tcaaaattcc gttcatttcc
    16321 ccaaagctga acggtttgag ctggaacgtt tctatcttct tcgcctgcag ttgatccatt
    16381 attagtagca tagtcgtaat ttcgccagtt cgctcccccg tttgtataat cactatcatc
    16441 gatcaagttc tctccgtcat atggcgagcc tttgtctcct tgatagcgtt cattgctttg
    16501 tgtccaattt cctaacactt ttgtatagtc attcgttgta aacttcgggt agcgatgctg
    16561 ttcgttttca acttctccgc taggatagat atattcatag gggtcttgcg tagctgttgg
    16621 cccaagacgc tccaaaatac cgttgaataa ctggcgttga atgcgctgga gtgcttgacc
    16681 gctgggtacg attggttcgt ccagcttttc catctcattt tcttcgctac gttgaatttc
    16741 agcggaatct tctgtatcag tttcattttc ttgtaattct tggttatcta ttctttcatc
    16801 aaccaccact tgaagaggta gttacccttc gttcacatcg gggatctttt ctgtttcaaa
    16861 aatcaatgtg ccggtttctg cttcttttga ataaactttt ccgacatacc attcttgacc
    16921 atcgatcgtc tcgactttta ggtcttctcc atcttcatca taggaggata gacctgttcc
    16981 acgaacgttt tttacggtac caatcccggc ggctgccgca tcgattgcta aacgtattct
    17041 accttcattg gcactttcct ttttttcaaa agccagatgc cattcaaggg tttcttcttt
    17101 ttcaatatac ttcccttcaa tttttgcata gtcctcatct aagaatacct catcctcctt
    17161 tgtttctgtt gactcagcag ctacatccag ggtcgtcata tgatagactg actggaaata
    17221 tggtccaaat agcaaactga cagttactaa ctgcaaaagc ttcaatttta accctttcac
    17281 atccttatac tcctttcaaa aatatttgtc ttctttcaca acaatcaaca aaacaatgcc
    17341 catctaatta taatttaatt taattttaat tttaaatcaa atgatatcct ttttagttaa
    17401 aaaacaccaa ttatacaaca tttattatta aaattaaatt ataaaatttt ataacgttct
    17461 catttaacaa aacttaagtt attaagacaa atgaataaac aactacgctt tcctcgaaac
    17521 aaaaaaataa agcacaaatg cgcatttacg cactcatgct ttattcgtac aattaattca
    17581 gataacggca gcgcatagga tgccaataac acttcctaaa atagctcctc ctaaaatgtc
    17641 agtaggataa tgtaaaagta aaaaaatcct cgaataagcg atcaaacaag caaataaaaa
    17701 ggctaatatt cctgcaactg gacttaattc gaacaacgca gctactgata aaaaggctag
    17761 cgctgtatgt cctgaaggaa aagaagaagt cttcggttta cttgtgtaaa ggacaagccg
    17821 acgatttttt tcataaggtc ttttccgtct tgtcgttttt ttgataaaaa gcaaaccaac
    17881 aaaaaaatag atcaaactgc cgataagaat ggctagacca tattctctag tatcaggtat
    17941 caagactaaa acagcagcaa ggagcaacca aataaaacca ccattagaga tattactgat
    18001 aaatttcaaa aaattgattt ttcgactatc cgttttccgt tgcttggcga acttgttgat
    18061 aatactatca tcgattttag caatcaaacg caacatgtta ttcctccttt ttaagaacct
    18121 tttcttgcgt acgatggata aaaagcagga cttgaaaata aaacaagaaa ctgaaaccta
    18181 ataaagcaaa atagattggt cctgcaaacc agaatgcgac aagaaggatc aacgtgcctg
    18241 ccaaatagaa aagcgcgctt ttgaaatcat taaaaaacaa gaaaaatgca gtaatccaag
    18301 aagcttcttt ttgttccagt tgaagcaaga ttttgtaact aaagagaagc gcgcttaata
    18361 gaagcaaaaa caacgtagta tacatcaatg tatagccaat tcccccaccc caatagcgga
    18421 aaatctgata atcgatcaat agaagaagaa aataaacact tgctaaacta tcttgctttc
    18481 cataaagaga gaagttctca cgaaactttt tccagaatag tgaaccgagc tttttctctt
    18541 catttttttt gtaatgataa atcatataca gaactgttcg acttgcagtt cctaatgtga
    18601 aaacaggcaa gacgcacagg aaccatagta gctgtaaatg gataagacgt ggaatccatt
    18661 caatgatttc taagaaataa tcattggctt ttttaccaat cgatcgattc atcatccttt
    18721 tgaacctcct gtcaaatcca tcccttcaat gaaataacgt tgtgcaaaaa agaacagcaa
    18781 aatcgtcggg atcaagacaa gtgttgctcc tgccatcaga taattccatt gtgtcgttgc
    18841 ttggttctga aaaacttgca agccaatctg taacgtgtat ttttcttgat cttggatata
    18901 taacagagga cctaagaaat cattccaagc tgcgttgaag gaattgatcg caatggtaat
    18961 caaggcaggt ttcgtcaatg gaagcatcaa atgactccaa atatacaaat gattcgctcc
    19021 gtcgatttca gcagcttcga tcaattcatt attgatcgat aagtaaaatt gacgcatcag
    19081 aaatatattg aacgcattgc cgaagaacga tggaacgatc aggggcaaat aagtacctac
    19141 ccagccgatc ttggagaata atacatattg agggatcatc gttacaaacc ccgggatcat
    19201 catcgtagaa agaaccaaag caaacatcag ctttttccca ggaaaatcaa gcttcgcaaa
    19261 tccataagca ataaaagaat ttgaaaggac attaccgatc actactagca cagtgatgaa
    19321 taatgtattt cttgcatagc gccaaaaagg aaaggcggca atcgtatcca gatagttttc
    19381 ccatacgatt ttttctggga aaaaggtcgg aggataacga acgatttctt ccattggttt
    19441 caatgaagta aacaccatcc aaagcaatgg aatgatcaaa atgattccta ttactgtcat
    19501 gaggacataa cttagtattt tttttgtttg ccgattcaca ctggtctttt tgaaataatc
    19561 catcatttgc cgtcacctcc agaataatag acccacttag gtgcaagctt caaattgaca
    19621 aacgtcagaa ccaacacgat cagaaacagg atcattgaca tagccatcgc ataacccata
    19681 tcaaaagcta cgaacgcctt gttccacata tgcagattat aaaaaagcaa agaatttgca
    19741 ggtccgcctg ttccattttt cgtcatgaca taagcttctt gaaaaatttg gaaagaaccg
    19801 atcgtagagg tgatcacatc gtaaaaaata ataggagtga ttaatggcaa agtaatactt
    19861 ttgaactttc cccaaacgcc tgctccatcg atttctgctg attcatataa agatctagga
    19921 acattttgta atgtagctaa gtaaagtagc attgctcctc cagaactcca tattttcatc
    19981 aaaatcaacg caggtttcgt ccattgtgga tcaaacagcc aagctggtcc agggatatgg
    20041 aaccagccca gaaatgtgtt gatcaatcct gcagatggag aaagcagctg catccataag
    20101 atgtagactg ctactcctga taagactgcc ggtaaataga aagctgtacg gaatacactg
    20161 attcctttga ttttttgatt gagtactgtg gctaaaaaca cactgaaaat cgtcgtcagc
    20221 ggtacattga agatgacgaa ataaacggtg ttgtataacg atttccaaaa taaatcatcg
    20281 tgaacgaaca ttcttttgaa atttgctaga ccaatgaaat tcatcttgga tgtcaaatta
    20341 taatcggtga aactaccaat aactgaaaaa agcataggcc ctagcataaa taccaagaac
    20401 ccgataataa aaggcgagat aaataaatag gcccatcttg cttctttttt ctttcggata
    20461 gataaacgct tcaaattttc ttgcctcctc tgtacctaga cagtcaaaaa tggattattg
    20521 attttgcttg actaagtttt ctactgcttt ttgtgcatct gccaatcctt cttttggtga
    20581 ttttgttcct aaaagaatct ggtcaatctg ttcattcact aatgtcgtgt aatccggagc
    20641 atctaaagga actggtgtga tcaccgtatt tttaaagttt tcgtccgcca tttgatagat
    20701 catttttcct ttatccgtta ggttgtcatt attcactaaa ttctcattgg ctgtcttgct
    20761 cgccatgata tcaaaacttt tttcgccgaa tttttcttgt acttctggag aagaaagata
    20821 tttgatgaat tcataagagg cctcaggatc ttttgctccg tatggtactt ccaagacgaa
    20881 tccgccaccc cacgaccaat ggcctgaacc ttcttctttt tctgggagct gcatcacgcc
    20941 aaaatcaaaa tcatccggtg cattttccat caaactagag taataattga tattttgggc
    21001 acgcatggct actaatccgg aaataaatgg atcagataca ccagacccaa attcggcttc
    21061 taatttattg atcgtatttt tcccataata atcttgccag tcttggatcc actccaaggc
    21121 ttccacttta tttttagtat caatcttgac tttttcatct ttgtcaaacc aactagtccc
    21181 gttatctgcg tttagcgccc atacatcagc ccctaaattc cataatggat aaaaaccgat
    21241 gcgtgtaaat tcttttcctt cttttttatc tagtttacgt gctgtttctt ctaattcatc
    21301 ccaagttgtc ggtagatctt gttcactgat tccagcttct ttaaataact ttttattata
    21361 aaaaatgact tgtgtatctg tattgaatgg gatcgcataa ggatcacctt catattctac
    21421 tgtatcccaa agctgcggat aaaaatcctt agaagtgtct ttttctacat atttggagag
    21481 attagttgct tgctgtgctt ctgctctttg ggcaacggaa tttatgtcct gtacaataac
    21541 atcgggtgca tttccagctg ttattgcaga taaagattta gtccagatgt caccccatgg
    21601 ctgatactta taatttacat ggatctcgtt ttgtgattga ttgaaatcct caatgatttc
    21661 ttcgatagct tcttttcgtg caccggatcc ccaaaaggac cagaaatcaa tattttttct
    21721 tccatcactt gttttttcag aagaatttgc tgcacttgaa ctggcatcgc ttgcattact
    21781 tgatccgcat cctcctaata aaaacaatgc tgaacttgcc agcaaccctg taaacaataa
    21841 ttttttcata ttttacctcc aaattttaat gtaaccgatg ccggttttaa tataacgata
    21901 tcattttgtt ctgaaaataa caatttataa ggtatgattc taaaaaaatt attaatgata
    21961 ccaatacttt atataattcg cgagcgcttt ctttgaattt tttaatcttt cgtagcagaa
    22021 tgagaatgta ttaaaaatag attaggagga attttgatgt ttagacacca gaaagaattg
    22081 cagtttgaag caaaaccaga tcgtccgaat ccattaatcg cgaaatatct gcaggagctg
    22141 atcggcggcc aatatggcga gatgagtgta gctatgcagt acctgttcca aggctggagc
    22201 tgccgaggag aagaaaaata taaagatatg ctgatggaca ttgcaacaga agaaatcggc
    22261 cacgtggaaa tgttggcgac aatggttgct cgtttattag agggtgcgcc tgttgaagat
    22321 caagaagcag cgatgcaaaa agatccaaca atcggtgcca ttatctctgg aatgaatcca
    22381 cagcacgcga tcgtagatgg cttaggacct cgtcctgctg acagcgtggg caatccatgg
    22441 agcggtgcct atatcattgc gagtggcaac ttgttagcag actttagagc taatttaaat
    22501 gccgaatcac aaggaagact tcaagtagca cgtatttacg aaatgatcga tgatcgtggc
    22561 gtaaaagata tgctgtctgt tctccttgca agagatagct atcatcaaaa tctatgggaa
    22621 tgtgcagtga aagaattaga agaaaaagaa ggttcaatct tagtcccaag tactttcccg
    22681 cgtgacaaag aacgaaaaga cattgcttat gacttctata acttctcaga gggagaccaa
    22741 agtagtgaag gttcttgggc tagtggcaaa gcactggatg gcgaaggcga atatcaatgg
    22801 ttgaaagaac ctaagatcga aggcgctgca ccaaaattag aaccagtaac accaaaaatg
    22861 tatgggacac ctccattcaa gaaataaata tctacccgta gaaacacccg ttttttgttc
    22921 cacacgcttt gtgagcaaaa ctgactaaag tcattaatca taaccaccct tcaaaagggt
    22981 ggttttctct agagctataa gctcaaatca ccggccagcg cctaaagacg ctggctttca
    23041 tattttatgt tcaagccata ttgcctttgc cagttttgcc cacctcttaa aaggtttatg
    23101 tactactcct tagcccttaa aaggatcgtc ttactctcgt acactcagct tatctaacgc
    23161 actatcatgt ttttcttgtt cttgaatgta ctttttgatg gtcgcttcat tttgtcctac
    23221 ttttgtcaca tagtatcctt ccacccaaaa gtgtcgatac ccaaatttat attttagatt
    23281 tgcacaatct cttgatgatc tcctgaatat tggctcgata ttgattataa attatttttc
    23341 ttctatactt tggtgtgaat acaatgtggt atttacacat ctactttgta cgggctaaac
    23401 tatttccctt tattgctcca cccgcatagc aagtggtttt gtatttcaat cgctcggcgc
    23461 gctcaattag gtctaaagac aataaatcta aaaacccgct gcttcactga cagaaacaac
    23521 gggttttcag aaaaaaatga aatgaaaaag atctcttacg gcaatctatt ttattttttt
    23581 gattttttat aaatggaaaa tgcttcaaga agtccaagaa ataaagccgt ataaaaacaa
    23641 gaacctatga aatctttatt tattagatat tcaacagaaa tcccgataac actagccaac
    23701 aatatattta tcagaagtgt aaaataagga tgctttttat agaatagaac tagtgttgtt
    23761 ttcatagact ctcctttctt catatttcca attaatcaca aattcacggc attactagaa
    23821 aaattaaagg ggcgcagaag tggaaaaata ctcttcgact ttccctacaa accaggccat
    23881 cttgtgttta ataactatac caaatctaag gtacctctaa atctaaattc catttgtggc
    23941 ggtgacctcc aatttttaac tcttctatct cccatccagt atacataacc ttgatacttc
    24001 gaccacttat atatcgaaca cacaaatacc tatccattgc aggagtacta cctaacttag
    24061 ttgctacttg catctaaata acagctgaaa agataggact tactcttagt aaagtatctg
    24121 aactattaat ttttaaacta tcatttttat cctcaactac atgcacatct gttgcaactt
    24181 cactttctgt ctcatccaga taaatgatta aggaatcggg aattgcaata tcttcagctg
    24241 acgctggaat agttgagcta aattagtcat catatcttta ttataaaata accaacagca
    24301 aaaaatacaa tgaaaaacaa acttggaact aatgtatcat taataaatgt tgaaaaattc
    24361 agcaactcat tactgtatga ttttagcagc ccaaccaaca tataccctat aaaagctaat
    24421 aagatggtta ggaatccttt agaattttta ctcattcata gacacttcct taaataactt
    24481 ttattataga aggtattgta aataataata ttgacaaaaa gtgactcaaa ataaaaaaaa
    24541 ccttacatta tactggaatg aactgtattg atagtgtacc taaaagctct actgacataa
    24601 gatcgtgtaa tcggtaaagt aattttatta attatacggg atgtaatcaa ttagagaata
    24661 aactaatcat aaaaggtact tttacattca taattgtcgc tttgagattc tactttggtt
    24721 cactatctga cccaattgca cattttcgat cattacaagt attggtgtcg ttagaacaac
    24781 taaataaaaa ctagcggtag atttttcaga atctatgcta atttctgtat ggatgttcca
    24841 gtgatggcaa atttctcttt tcattgtttt tcttgtcgca tgatatgcct ttttgtgtta
    24901 ttcagttaaa ctatagaaat tctttcctag acagatcttg atcccaaatt ttacgttttt
    24961 gagcatcgat atagtttaaa atgacattac tcatatctaa agagtttaga ttttttagga
    25021 ctaaaactgt cagttgttct ttttcttctt tctgttaaag tattgtcgcg acttttcgta
    25081 tacgttaaga tatgtcgtga aaaaacaagt tttgtgacac aaaaaaacga atgacttgag
    25141 gaaatcacct caatcattcg tttaaaatgc tatatattct ttagtttaag atacggaaat
    25201 taatgccatc gaccacacta gtagaatgaa cgacatttac ttggcctttt tttaatttcg
    25261 ttatgtttag catataaaaa tattatagag tttacttaca atgcttaaaa catatactgt
    25321 ataatctgat caattttagc catgtcttgc aatggtaagt tttctatttt tttgagtttt
    25381 cgttccttaa aatcaagaga ttttaattgt gtaataatta cttgtccttt tatatctagc
    25441 tcttcaggta atgagtaacg tgtcggtaaa ttctttatag tggaagtaat tggacatatg
    25501 accgaaaaca ttgttttacg gttaaattca taacgagaga cgactagtcc tggtcgtctt
    25561 ttttgaattt cttttcctac ggagggatca aaatcaatcc atacaatatc acctttttta
    25621 ggaatataaa ttctttcttc tgacactata aaatttctct cccttcagga gttaaatctt
    25681 cccattcgtc tttctcataa tactcggcct cttccccacc actaaatgga tcttcaattt
    25741 ttggaactaa cgtaattgtt ccatcatcag aatacacaac gatatattct tggttttctg
    25801 aaggtttctt tccgttatca gaaggtaaag ttaccactac agaacttcct tgtaatctag
    25861 attttgtaac aagcattatc atcactccct taataaaagt atactatata acactaagta
    25921 acgcaaaatt cccctttgta aactatctag aaaattgttt tgtttaaagt atatcttatt
    25981 ggatataaat agaaataaat tgtagccagt tcattttttc tttaactaaa aaaataatcc
    26041 aagaaacatt gaacctatgg atccaaaagc tacccaaata ctatagctaa ccccccgcta
    26101 ccttcaattt tgcagataaa acgctttggg agaaaatttc aaagacaaca ggccatccta
    26161 tcatgcccga ttataaacaa aaagctggct attccttttg ctctgacaaa tttcaattgg
    26221 gaaagttcac gaaactagca ggtgaagaag tttcaactgt tcggatcaaa gaatgcccgc
    26281 ttcaaataga aacattggtt caacgaattt caactagaca agactttgct attgtagaac
    26341 gtgagatcgt agctattttt gtagaagaag ggattttaca tgatccaact catatcaatg
    26401 tggataaatg gcaacctctt atctataaat tcagagaata tacaactgcc aaccagtcgt
    26461 taactttgaa ttttagattc caagaattca aagagtcata atgataaaca tcgttaggta
    26521 taaataaaaa agactcgcag cacgagtaaa aaacaaatag aagctataca ttagaaaggt
    26581 cgactgtgac cctatagatg cagtatatgc agcttatgag catttcttct gttctctttg
    26641 tttttgggat ctgtgctgaa gtctttttcg ttttttgccg ctctttcttt gaaaaatcga
    26701 ttatgttata ccttctccat tactaattca ggcatctgct gagtaatgat ttttcgcgca
    26761 aaagaaatat ctgttaccca aggttcgatt tgttcttcag tcaagacaag cggcattcgg
    26821 tcatggattg gtgcgactgt gcggttaggt tcagtagtca ttatgattga ttctgccatt
    26881 ttttcatcct gatggctacg ataaaaaccg ccaatataca ctattttttc cttatttgaa
    26941 aacataaatt tgtctttttc ttcattccac tcattgggaa aacacaacga ttttgcaaaa
    27001 atggttgatg aaaggtcttt ttttcttcta ctgtctctgc acgggcatta atcatcaatt
    27061 gtcctttttt aaatccggta aatccccact tcatgatttc cggagtgagg atgttttctt
    27121 cctcttttct taacgtcaca acaagatttg acggaaaaac ttctccagct gctaattctg
    27181 ctgcctgctt cggttgtttc gtctgggctt ctcgataata attctgaagg agctctgact
    27241 ctagatcaaa aaaataacga ccacacatcc taatccctcc caatcttttt ttctatcata
    27301 cactgcaaca agaagttcgc accaacaaaa cgctttcaat ttttgtagaa ctaaaaaagt
    27361 tgtgacaagt gacaaactct ccagtgaatg gtattctcct gccaactcct cggtcacaag
    27421 tcacaatatt caacaaacat gagaagctgc tttttgaaat tgttccttgt gactcggagt
    27481 gaaacggcag gcttacgacc tctgggtaaa cggtgttcaa aagccagttt ctcggtatta
    27541 agccaaattt atgcaaaaat gtagaaagct attttcacaa aatttttctt a
//
LOCUS       DAYYGS010000034        27470 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000034 DAYYGS010000000
VERSION     DAYYGS010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.034"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA44_001325"
     CDS             complement(41..3694)
                     /gene="rpoC"
                     /locus_tag="ACHA44_001325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFM8904621.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA44_001326"
     CDS             complement(3773..7390)
                     /gene="rpoB"
                     /locus_tag="ACHA44_001326"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8904622.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8118..9086
                     /locus_tag="ACHA44_001327"
     CDS             8118..9086
                     /locus_tag="ACHA44_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8904623.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9104..10009
                     /locus_tag="ACHA44_001328"
     CDS             9104..10009
                     /locus_tag="ACHA44_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904624.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            10006..10812
                     /locus_tag="ACHA44_001329"
     CDS             10006..10812
                     /locus_tag="ACHA44_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904625.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10969..11937
                     /locus_tag="ACHA44_001330"
     CDS             10969..11937
                     /locus_tag="ACHA44_001330"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HFM8904626.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDVKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12254..12778)
                     /locus_tag="ACHA44_001331"
     CDS             complement(12254..12778)
                     /locus_tag="ACHA44_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HFM8904627.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13065..14876
                     /gene="scm"
                     /locus_tag="ACHA44_001332"
     CDS             13065..14876
                     /gene="scm"
                     /locus_tag="ACHA44_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HFM8904628.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDTS
                     ASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(15031..16017)
                     /locus_tag="ACHA44_001333"
     CDS             complement(15031..16017)
                     /locus_tag="ACHA44_001333"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFM8904629.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(16040..16606)
                     /locus_tag="ACHA44_001334"
     CDS             complement(16040..16606)
                     /locus_tag="ACHA44_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HFM8904630.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(16620..17036)
                     /locus_tag="ACHA44_001335"
     CDS             complement(16620..17036)
                     /locus_tag="ACHA44_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HFM8904631.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(17493..19427)
                     /locus_tag="ACHA44_001336"
     CDS             complement(17493..19427)
                     /locus_tag="ACHA44_001336"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8904632.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            19729..20664
                     /gene="pplA"
                     /locus_tag="ACHA44_001337"
     CDS             19729..20664
                     /gene="pplA"
                     /locus_tag="ACHA44_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular electron transfer flavoprotein
                     PplA"
                     /protein_id="HFM8904633.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            20754..21857
                     /locus_tag="ACHA44_001338"
     CDS             20754..21857
                     /locus_tag="ACHA44_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFM8904634.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            21870..22823
                     /gene="menA"
                     /locus_tag="ACHA44_001339"
     CDS             21870..22823
                     /gene="menA"
                     /locus_tag="ACHA44_001339"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFM8904635.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACHA44_001340"
     CDS             complement(23637..24983)
                     /gene="gor"
                     /locus_tag="ACHA44_001340"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFM8904636.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGIGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(25117..26370)
                     /locus_tag="ACHA44_001341"
     CDS             complement(25117..26370)
                     /locus_tag="ACHA44_001341"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFM8904637.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(26376..27299)
                     /locus_tag="ACHA44_001342"
     CDS             complement(26376..27299)
                     /locus_tag="ACHA44_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFM8904638.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPSEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
BASE COUNT         8020 a         5822 c         5209 g         8419 t
ORIGIN      
        1 gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt tgattgttca
       61 ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca ttttcgcttg
      121 ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta ccagctggga
      181 tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc ttaccgcgga
      241 tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat aagaaactat
      301 ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt gcaggaacgc
      361 ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct aataatgtac
      421 ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc atttgacgaa
      481 ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa acacgttgta
      541 cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac aattgttttg
      601 gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct tctgctactt
      661 tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct ttgattgtta
      721 cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct gttacttctg
      781 tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca cgaggaagac
      841 cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc gtcaactgag
      901 taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg acttcaacgt
      961 cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt tttgtattac
     1021 atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt tgttttgcga
     1081 tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca ggatgacgta
     1141 cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg atttcatttc
     1201 cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct tcacggatga
     1261 ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg gcagtcttca
     1321 aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt tccaagacgg
     1381 atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca ttaggagcgg
     1441 ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca cgagctccag
     1501 aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt ttttgttgga
     1561 tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg tcatctgtga
     1621 tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca ttttcaatga
     1681 tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata ccggcatgtg
     1741 tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt tctgttactt
     1801 tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg aacggtaata
     1861 ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca aagtatttgt
     1921 ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg aattcaggag
     1981 gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt tctttttgcc
     2041 attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt gaatgtaaat
     2101 gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag accattcctt
     2161 cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc atatcttggg
     2221 aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca gctaacatca
     2281 acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc atttggtctc
     2341 catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt gcgcgtcctt
     2401 gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt gcccggttca
     2461 gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg tcttcttgac
     2521 gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga acaagctcac
     2581 gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca cattggtaca
     2641 tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca cgtttaccta
     2701 gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga gatttcaatg
     2761 ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc aatgcatcta
     2821 ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca ttcaagtcta
     2881 acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca ttcaagtcac
     2941 ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt ggaataacag
     3001 ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat gcttctaaaa
     3061 tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc aattcttctt
     3121 tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta atcgcttctg
     3181 cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga tattcgcgtt
     3241 ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact acatatgaag
     3301 caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga cccatacgtg
     3361 aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca atatgtccca
     3421 tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca caaacgattc
     3481 ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt gtaggaccaa
     3541 aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag ttaatcgtct
     3601 caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa gccaaaccta
     3661 tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat ttcatttaat
     3721 ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg gattagtcta
     3781 aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct tccacttgtt
     3841 cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt gtcaaagcat
     3901 caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc tctgtttctt
     3961 cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc aatacgcgga
     4021 atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct tcgtatgttt
     4081 tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa gtataagccg
     4141 caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca ccaaattgag
     4201 ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa cgtgcatgca
     4261 atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca gacacacgtc
     4321 catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg ctggccatac
     4381 cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat actggcgttg
     4441 ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat acttgcccga
     4501 tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt gttccatcag
     4561 gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta tttccgtgac
     4621 gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa acacgaacta
     4681 acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt ttcacatcgt
     4741 gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact tcacgagctt
     4801 tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt accccttttg
     4861 gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata cggatgatac
     4921 ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg cgtgtgattt
     4981 cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata tggatagaag
     5041 tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca tagttgtacc
     5101 cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg ccttgttcca
     5161 tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa tgaacgattg
     5221 gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca tcgtatttat
     5281 ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat tcaacgacac
     5341 cgtcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat tccataccag
     5401 taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc atgttggcac
     5461 ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct gtcgcaacgg
     5521 caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat acttctaagt
     5581 tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct tcattcaatg
     5641 ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag tagtcgattt
     5701 ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg ataaatccga
     5761 atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt gggccttcag
     5821 gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga acttcataac
     5881 cggcgcggtc acgagtcaat ccgccaggtc ctaaggctga aagacgacgt ttgtgtgtca
     5941 attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa ccaaagaatt
     6001 ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc aatgtttcag
     6061 tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc ccgatacgga
     6121 attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag tggtcgatat
     6181 catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa gcaatgatgt
     6241 cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc acgtttactt
     6301 cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt ggatctgtga
     6361 ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca atgaaaggag
     6421 ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg atctcgcctg
     6481 tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt ttcaaatcta
     6541 attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt ggatcgaaga
     6601 aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct ggacgcaaac
     6661 gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt ttatgcaagt
     6721 ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag atcgtatcat
     6781 cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt gtacggtcga
     6841 tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct ccacggttag
     6901 ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca tggaaataga
     6961 cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg atgatgaatg
     7021 ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct ttgatttcgc
     7081 cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag tttgcatcat
     7141 gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct acaaattcca
     7201 aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt tctctaagtc
     7261 cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt ggtaattcca
     7321 acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat tttactacgt
     7381 gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc gatcaattgt
     7441 tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc ggcttttagg
     7501 caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc ttctactttt
     7561 gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc ttcatcagat
     7621 agtttttgca acttcatata ctacacttat tttctataaa agtcaacact tcctggcgtt
     7681 tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc aattatctga
     7741 caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc attttgtgga
     7801 ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa taacttttac
     7861 tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta aacgacagtt
     7921 tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag acaaaatgct
     7981 aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt tttcaattta
     8041 ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg tttcccaaaa
     8101 ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct gccctgctac
     8161 tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat ggacaaaaaa
     8221 caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat aaaggattca
     8281 aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc gattatcaaa
     8341 atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc aaagaagatc
     8401 ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat gaaacaacca
     8461 ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta gtcacatcca
     8521 atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc gaaaaacaaa
     8581 tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg tataacaacg
     8641 gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa aaagcgggcg
     8701 tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg acgaaaagtc
     8761 tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca agtgcggcaa
     8821 gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc gcagttgatc
     8881 aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg ggtaaacaaa
     8941 cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt cctgtcgaaa
     9001 cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta ggcatcaatc
     9061 cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa atttgctctc
     9121 tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga ttggtgtata
     9181 cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct ttcctttagg
     9241 tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag cttgtcttct
     9301 aggcttttta gggggtgcgg cagcagggct tgtttccgga ctgcttcaca caaaattgaa
     9361 aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg tcacatcccg
     9421 tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc attgggtcga
     9481 gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt ttgctttgat
     9541 cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta ttcgtgcaac
     9601 tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata tgaagatcat
     9661 cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg cgcaaaacaa
     9721 cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg cttctattat
     9781 catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga ctgtcgtttt
     9841 aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg ttgaaccaag
     9901 tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc cgcagatcca
     9961 gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa tgacgtctta
    10021 acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa tcatgtcctg
    10081 aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg tggaaatggg
    10141 gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga tcagggtcaa
    10201 attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc gaaatccatc
    10261 agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt agaagaaaat
    10321 cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt caaaccacaa
    10381 gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga aaatcgttta
    10441 tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt attgatggcc
    10501 acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct tgaccctgct
    10561 acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa tctcactgct
    10621 tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact gatcatgctg
    10681 catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct cacagtgccc
    10741 caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga tcaagtactt
    10801 ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct cttctttttt
    10861 ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga aaactatttt
    10921 gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat gacaacgaaa
    10981 gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga aaagttggca
    11041 cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt ggaaaaggca
    11101 ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga cgtactagat
    11161 gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt acaaacgatg
    11221 ttgacttctg aatctacgat gaaagatgtc aaactggcag cagctcaagg agattctgtt
    11281 tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg acggcctttt
    11341 ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga caaacggttt
    11401 gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc cattgaatct
    11461 gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga tggaaaaaaa
    11521 gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat cagtcatatc
    11581 atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc tgatctccaa
    11641 gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa tcagctaatc
    11701 agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc gttggacttc
    11761 tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa aggaacgact
    11821 tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca aacaaatgaa
    11881 cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac gcaataatta
    11941 tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga attctcttca
    12001 aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa aggtattcta
    12061 aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa aacaagagag
    12121 tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat cttatagata
    12181 gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg taagttcttt
    12241 tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc acctgaccag
    12301 agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg actggtggtg
    12361 tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt aagtaagaaa
    12421 tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga ttagcttgga
    12481 tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct atcgtcatcg
    12541 gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct aaatcagcaa
    12601 tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat ttgatctgcg
    12661 gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct tcaaacaaac
    12721 gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa taatccattt
    12781 attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata cctacttata
    12841 tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat agtttggaga
    12901 ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat atgaacgttt
    12961 cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga ctaggagaaa
    13021 aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa aaaagggcaa
    13081 gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg gccgtttcag
    13141 cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact ggtaactatg
    13201 gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca actgtgaatg
    13261 cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat tttggtacag
    13321 agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact gattctttcg
    13381 ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc gttggcagta
    13441 acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg cttgatcact
    13501 taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat ttcgagcagg
    13561 aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca gctaaaacat
    13621 atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact gatttatcag
    13681 ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat acgccacttc
    13741 aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt tcttctgcat
    13801 tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca gaagattttt
    13861 cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca atcagtgctc
    13921 cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa aaccaagacg
    13981 gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg gatcctaacg
    14041 aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt acagatacat
    14101 ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat actacctcta
    14161 ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact acctctacca
    14221 caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc tctaccacaa
    14281 gtgacagcac ggacacgtca gcttcaagcg acagtacaga tactacctct accacaagtg
    14341 atagcacgga cacatcagct tcaagtgaca gtacagatac tacctctacc acaagtgata
    14401 gcacggacac gtcagcttca agcgacagta cggatacatc taccacaaca agtgatagca
    14461 cggacacgtc agcttcaagt gacagtacag atactacctc taccacaagt gatagctcag
    14521 attcatctac cactacaagt gatagcacgg acacgtcagc ttcaagtgac agtacagata
    14581 catcctctac cacaagtgat agctcagatt catcaacttc aagcgatagt acagacacgt
    14641 cgtctaccac aagcgataca cgtcatgcaa gtgtctcctt ctcaaaaaca agttcggata
    14701 acaggacgaa ctttaactta actaataaaa caagcaatga caatggaaac aaatcaaaaa
    14761 gagcacttcc taaaactgga agtcaatcga ataattggat aacactcgca ggcgttatat
    14821 tacttgtaat cggtttaaga atcactttta tgagttataa gaaatcgaaa aggtaaaaaa
    14881 atattcaaga taaaaaagag caacgtcgct aacggacgtt gctctttatt tttgcttttt
    14941 atcataaata caagaactac aattcaattt gtcaaaaaac acgtagattt ccttctgaga
    15001 aatttattta cgtatcgaac ctttccctat tcagtctttg cgatccaaaa tcagttgggt
    15061 aagggaaaag agattctctt ttgttcgaag agaggtggca ggtaatttct ttatttcctt
    15121 caaagccttt tctgtgtaag aggccgctaa cttcctagct tcttccacgc ctcctgtatg
    15181 ttcaacgatt ttgcgtacgg cgtcgacatc ttcttctgtc attgccgctt tcttctctaa
    15241 ataaggtagt agctttggtt tgtttttttg caaagagtag atcaacggta aagaatagac
    15301 cccttgacgc atgtcttcta gtacaggttt gccgatttcc tggctctctt ttgtataatc
    15361 taaaatatca tcgataatct gaaaggccat cccgatagaa agtccgatat ctcctgcttt
    15421 ttttgcgaag cgctcgcttg ttccactttc aaaagcgcca acagaacaac ttaattggaa
    15481 cagttcagct gtcttacctg aaatattttc caaatattct tttacagaaa gttcataatt
    15541 atagcgatca tccatctgcc ctaattcgcc attcaatact ttttccatac tgcgagaatt
    15601 caattggaga cttttcaagg aagtggagta atcagacaat agtttaaaac agcagacaaa
    15661 taaatagtct cctgcataga cagaaacgac attaccaaac tgactacgga tattaggctg
    15721 accacgtcgc agatccgctt catcaacgat atcatcatgg atcagtgtgg ccgtatggag
    15781 catttcgata gaagctgcca aagcgatggc tttgtctcga tcttgctctg gtccaaattg
    15841 tgagaacaat agctgatagg caggacgcaa cagctttccg ccggcatgga tcgtttcttt
    15901 gattgcttgt tcgacaggtt tgtttttcag gcggacagtt tcttccatcc gttttagtgt
    15961 ttctgtcaat tctttactta aagcaggata gttttcccac attggatgta gcttcataaa
    16021 aaaactcctt gtgtgacgat tattttttgg taaacattgg gctccatttt tgtttggttc
    16081 cctgctcata atctttttgg aatcgttgaa gtgtctgcac atgtcgtaaa agataattcg
    16141 ctgcgatccc gattgcaatc cctgataaaa tccctaaaac tgaaaggact ggcaagtaca
    16201 acatgactgt ccatgattgt gcaatgaaag aagccatcaa aagctgtccg acattatgca
    16261 tgaaaccgcc ggccgcacta attccgatga tactgacacg ttttggtcct aattgcttga
    16321 ccaataacat tccaaaataa ctaagcaaag caccactcat actataaagg aatgtggaaa
    16381 tcgttccgcc tagcaaagta gtcaggaata gtcgtagcca aagtagtaag aagctgtctc
    16441 gttttttcat ggtaaaaatg gcaacgatcg tgattagatt ggctaagcct agttttgcac
    16501 caggcgcaaa agaaaaagga taagggatca tattttccaa taaaccaatg atcactcctt
    16561 gtgcaacaag tagcgaaata taaatcattt tttgtaattt actcatggtg tgtgaaggtc
    16621 taataaatca gactgtcttc gtcacctcca tctgttgaac ggacttcgat cactaattta
    16681 tgtggcaaac agacgatggt ttcgccattt ttcgttgccc agcctcgacg tacgcagatc
    16741 tggtcatcgc aatctgcttc ttttatccgt actcgatcgc catctatctc gatcaagtta
    16801 taatctccgt gttcatcttt gtaagtatat gtgttgctct cttctccagc catcaaaggg
    16861 aatgttttga tttctgtgcc atccacacgc agtaccgctt ctttttctgc tgttgcggtg
    16921 ttttgctgaa gaccaaaaat catgactggt aaaaaagaaa gaacagtcaa aagcaaaata
    16981 ataaaaacat cccaaggttt gagcagactt ttttcagcaa attctttaag gctcattttt
    17041 gttctccttt ttccagatta actcacccaa ttttagcata aaaacaagag gttgtgacac
    17101 ctgtgtcttg ctttgagtaa taagaacgaa gaatagaaaa cagctttcca tgttttctaa
    17161 ttctttgggc ttattaccga gaagcagcag gtggaacacc gtttataaca agaggttgtg
    17221 acagaagtgg gcaacttcaa gaaataagaa ggaatttacg aaaattactt ttcaagtttt
    17281 tgtgaaattt cggcttattt ttcgaagaag ctacttctgg aacaccgttt ataacaagag
    17341 gttgtaacag aggttgtgac acctgtgtct tgctcatgga ggcttatctc gggcatctta
    17401 ttcctttaat tttacataaa aaggaggctg tgacggtttg tctcagcccc tttctattga
    17461 aatcttgcat tcaaataacg ttctttatct atttatttct tgccgtactg ggaacttggt
    17521 ctaccgtacc accaattacc tagtgtacgt tgtagccatg gaattaccca gcgatccaac
    17581 ccaatcgcac gtccggaacc gttcatcaat gcgaaagcaa caaaaatgaa ccaaatgttt
    17641 acccagtaaa acatgcctga taagcagaac gtgatcacca atacaatcgt tgtcgcactg
    17701 catagccatg tgaataatcc agcaatcaat gctaatgcca aggcaatttc aaagaatacc
    17761 atgaattttt ggaagaacat tgctacatca ttgtttggca tgatcacttg catgatcttt
    17821 gtgaaccagt ctggtgactt ctcaatcaca gccattggtt cttcaccgta tgcataactc
    17881 aaaccaaaat gtgcagcttg agtcgctgct tctccggcac ttgcccctgc tccgctggca
    17941 gctgtggttg catcaccagc agcagaagca ccactggcaa catctgttac attgtattcc
    18001 catgtccaag ggaacacact gtttccagtg aaccatgatc cttcaccaaa ccaagtactt
    18061 ggtttgaaca cttcaccatt cccaatgatt tttttggaag cttcaacgaa ccaaactaaa
    18121 ccatagaaaa tacgaagtgg cacactccat aagacattgc cgtaacgaga agaatgtcct
    18181 cgagtcacgt tgcgatcatc tttgatatgg aaaaactcat gcattaaata ttggaacata
    18241 taataaccag aacgaatatc aaagaagtat ttcaaattca cgatatgttt cattacgata
    18301 gctaaaaatc cgcttaaatg gatcttatca aataggttag ctacccccca tttggcacca
    18361 atagaaacca taaatccttg atagtttcct ttaaatttat gcttttctcc gcctttgatc
    18421 gaagccacga tattcgctgc ggctgtgtgt cctgtttgtt cagcagcttg aacgatctgc
    18481 ggtgtcggtg ttcctgggaa ttcttcataa aagactaaat caccaatgat atagatgttt
    18541 ttatcttcgt agccttttgc ctgcatatat tcattggcga tcaaccgctg tccgcgtgct
    18601 gattctaatc caaagtcagc agcgtcagaa gttcctttga caccagctgt ccaaatcagt
    18661 gtgtgagtcg gaatgttgcg tccgtctttc aatttgatat gatcagcagc cacttctacg
    18721 attggtgcat tcaataataa ttccacgttg tgcttacgca aatagcgttc agcttttgca
    18781 gcatcatttc gatccaacat attcaagatc gtcggcattg cctctacgac catcagtgtg
    18841 atttcgtttg gatctaattt ataatctttg gctaggcgat ttttccagtc gatcagttcc
    18901 ccaatcattt caataccggt aaatccagaa ccacagacaa cgaaagtcag catggcttta
    18961 cgcagttcat catctggttc caatgcagct tttgctactg tcttctcaat atgttcccga
    19021 atccttacag aatcttcaaa tgaccaaagg gtgaatccgt gtgctttgac accaggtgta
    19081 ccaaagtcat tcggctctcc gcccattcca aggatcagct gatcaaaagg ataggaacct
    19141 aatttggtct tgacgatttt tcgttcttta tcgataccga ttacagtgtc tgtgacaagt
    19201 tgtacattct tttgacgagc aaacaaatgc tgcagatcgt attggattgc ttctggttct
    19261 acacgtccgc cagctacttc atgcaactcg gtcatcatag tatggtacga atgacgatcg
    19321 atcagagtaa ttgctacatc ttcttttttc agtttctttg ccaagtattt tgtagctgaa
    19381 acaccggcat atcctgctcc tacaacgaca atattttgct ttgccattga tcatccgctc
    19441 cttatagact atctatataa tgaatatatg ataattcttt tagtttgata cttattacac
    19501 aatctccata tattatatag atgttctgtt cttttgtcta actccttttg gatttttttc
    19561 aaattttaaa ataaagcgtt ttttcacgaa atttgctgaa agaaaacgtt tgaaatatta
    19621 catgggttat ttatcaaaaa tagttgactt ataggctaaa aagattagaa tgtataatga
    19681 atcacaaaga aaaattgtgg actagaaatg gaatggtggg gtattaaaat gaagaaaaaa
    19741 cgttttgtaa ctggatttgc cgtattagca ttttcagctc tagtgttagg cgcttgcggc
    19801 gcagatgaca acggaagctc aaactcttct tctgaatcat ctacagcaca atcttcgact
    19861 gcaaaaagca caacagaatc atctgcaaaa gttgtagctg gcggagattt gcaagacggc
    19921 acatataaat tagaagaaaa aaattattca aatggttacc gtgcagtctt tgaaatggtt
    19981 gtaaaagacg gcaaaatcac tgaatcaaaa tatgataacg tcaatgaaaa cggcgaatca
    20041 aaaacaaaag atgcagaata caacaaaaac atggaagcaa aatctggcac aaacccagaa
    20101 aaatacattc ctgagttgaa tgaccaattc ttaaaagcac aaagcgcaag cggtgtggaa
    20161 gtcgtaactg gagctactca ctcatctgaa agcttccaaa actacgcaca acaattgatc
    20221 caagctgctc aagctggtaa cacagataca atcgaaatcg acaatggtgc tgatttgaaa
    20281 gacggcactt acaagcttga agaaaagaac tactctaacg gctaccgtgt gcaattcgaa
    20341 atgacagttg ctggcggcaa agtaactgaa tcaaactttg attatatcga caaagacggt
    20401 aaatcaaaac aagatgatac agaatataat gaaaatatga aagcaaaaag cggtacagaa
    20461 ccaaaaactt acatccctac tctgaacgac gaattcgtca aagcaatggg cgaagaagat
    20521 ggctcacctg ctaatgtaga agttgttaca ggtgctacac acagctcaca ttcattcatc
    20581 atgtacgcac aacaattagt gaatgcagct gaaaaaggcg acactcaaac aatcgaagta
    20641 gataatatcg taacagaaaa ataatcacat aatcatacta ataaaagttg gagggtggga
    20701 cgatagccag attaggcagt cgtctcacct ttcaaaatga agagaggaaa aagatgcgga
    20761 aaaaactact attttcctta atcacactgg gcatctcttt tatggtgcta actggctgca
    20821 gcaactcagc aaataaaaca accactgaaa gttcagcagc taccaagata ttgaaagatc
    20881 catattctga tgaacagttc ttgttaggga cttacgttcg tatccgtgtg tatgatgaag
    20941 gcaaagaatc agcattgaaa cctgccttcg atcgagtaaa agaacttggt gataaaatca
    21001 cgatcaatca aaaaggttct gagatcgatg aagtgaatga gcaagctggg atcaaaccag
    21061 taaaagtttc tgatgacgtc tacacgttag tcaagcgggc ctacgaatat agccaagatt
    21121 ctcaaggcgg attcgatatg gcgattggtg caatcacaca attatggcga atcggttttg
    21181 atgacgccag aaaaccttcc caagaagaaa tcgatcaggc attgaaatta gtggattatc
    21241 ataagatcga actaaatgac aaagaaaaaa ctgtttatct aaaagaaaaa ggaatgatca
    21301 tcgatttagg tgcaatcgcc aaaggttaca ttacggacga agtagtgaaa gtcttgaaaa
    21361 aacaaggagt gaccactgcc atcgttgact tgggcggtaa tgtctatgtc ttaggccaca
    21421 gcccaagagg cgaaaaccaa gactggacag tcggcatcca agatccgaac atggctcgcg
    21481 gatctgtttt aggttctatt aaagaacgga acaaaacatt agttacctct ggaatttacg
    21541 agcgttattt agaagtagat ggcaaaaaat accatcattt gtttgatcca aaaacgggtt
    21601 atccgttcga caatgatatc gcaagtgtca cgatcataac agataaatca attgatggtg
    21661 acgggttgtc tacagcagtc ttttcaatgg gtgtgaaaaa aggactggaa tacgtcgaaa
    21721 gcgaattgaa taacgggacg gaagcgatct tcgtgacaaa agacgataaa gtatatgtga
    21781 ccgatccaat caaagacacc ttcaaattat cggaggactc ccattacacg atgggtgacc
    21841 gttctgaact aaaataagga gtacgaacca tgacactaaa aatcttttta gaagtagttg
    21901 aaatacgaac aaaggtggct agtgtatttc cttttgcggt aggggtatta ttctctatcg
    21961 ctttttttca tgagatacat tggctaaaca cttttttatt ttttattgga atgctagttt
    22021 ttgatttagc gactaccgct atcaataatt acatggatta tgaaaaggct cattctgaag
    22081 tatataagta tgaagaaaat gtcattggcc gcagaaatct atctcctgta ttgattcgaa
    22141 acatgatttt agctatgatt gcatttgttt ttgttatcgg cgttattcta acgatcaata
    22201 cgggttggct tttacttttg atgggattta tctgttgttt tattggagta ttctatacat
    22261 tcggaccgat tcctttatct cgaatgcctt taggggaaat tttcagtggg gtgactatgg
    22321 gccttggaat ttttgccatg gtcgtttatg tcaatacatt cggttcagat gtctatgatc
    22381 tgacagtcaa cttttcatct ggacaatttg ctttggtagg aaatctttgg gctatagcag
    22441 caatcgtatt agcttcctta cccttggtgt ttacgattgc caatatcatg ttggctaata
    22501 atttgcggga tcttgaccgt gatattgaaa atcatcgcta tacactagtt ttttatatcg
    22561 gacgtcctgc gggaacagtc ttattccagc tattgatgta tgcttgctat ttcgtgattt
    22621 taatggggtt gttgagcggt atctatgaat ggccgatctt ggtcaccttc ttgaccttgc
    22681 caattgtgaa gaagcatttg gatcaattca agcaaacgtt accccagcct atcagtttca
    22741 gctattccat caaaaatatg gtcttattca atgccagtta tgtattgggg ctattactaa
    22801 caatcattat gcaagccatt taacagcatg aagaggttgt gaaaaaactg ccctgcatca
    22861 agtaataaga acagccaaat aaaaatagct ccttatattt ttcgtttggt tgggcttatt
    22921 accgcagatg caagcttttg aacaccgttt atcaggaggt tgtgaaaaag tcgcttagtt
    22981 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca tttttgcata aatttggctt
    23041 aataccgaga aactggcttt tgaacaccgt ttatcagagg ttgtaaaatc agtgttttgt
    23101 cctgagtatt agagaaacaa atggaaaaat agttcctcat attttgccgt tttttgagct
    23161 aataccgaag gacagctgat tgaacactat ttgttcggaa aaagtgacta actatgacac
    23221 aatttttttc acagtccctt ttttcacttg atttgattcg taccagcgaa cttgacgaga
    23281 actttcttgg tttggtaact atatggtatt cttttaacat cctcgtttgc ttcttctaat
    23341 ttatttatac aaagtcacac ttttttaaac atctgttctt cgatacattt ctatttcttt
    23401 taaacatcga taccaatatt ttgaacttaa gagtgcatac caaatggatt catttactct
    23461 tcgtcagtta cgaagataat caaaatctcc aacttcccta cttatttcgt tttgagaatt
    23521 atcgaagcaa tcttacataa ataactaaaa aacaaaaaac cagaaaatca gtatactgat
    23581 tttctggttt tacatggttt gttcaataaa aagctgttgg aactgttaat attaaattat
    23641 cgcatcgtta cgaattcttc cgctcctgtt ggatggatgg ctactgtatt gtcaaagtct
    23701 tctttagttg cacccatctt gatagccacg gcaaaacctt gcagcatctc atcgacacca
    23761 ataccaatag catgtaagcc gacgattctt tcttcttttc cgacacagat caatttcatt
    23821 tcgcattttt ggcgataatc atttaatgca aagtacattg gggtaaagcg tgaacgataa
    23881 actttcaatt cattttcgcc atatttttcc aatgcttctt cttctgtcaa accaattgtt
    23941 gcaattggtg gatgcgtaaa gacgactgtc ggaacaagtt tatagtcaag ataagaatct
    24001 ttctttccat taaataaacg ttctgataaa cgtctaccag ctgcgattgc aacaggagtc
    24061 agatctaatt taccgatcac atcaccaaca gcaaaaatat gttcatccgt tgtattttgg
    24121 tatttatcta ctttgataaa cccttttttg tccaattcca catttgtatt ttccagacct
    24181 aatttttcgg tattcggtac acgtccaccg gcaaaaagta cacaatcacc agttaaggtt
    24241 tcaccatttt caaatagcac cgtatattcg tcattgtttt tagtaatttc tttagcaata
    24301 tgatgtgcgt aagtttttat tccagcttct tggtacattt ggatcaaatt gtccgaaagc
    24361 attttatcaa acgttctaag tggtcgttct tttcgatatg cccacatgac ttcactacct
    24421 aaaccacgaa atacacctga aagttcagcc gcaatgtatc cagcacctaa aacgattgct
    24481 ctcttaggta actctgttaa ttcaaaaaat ccatttgaat caattgcgta ttctccacca
    24541 ggaatggtca ttttacttgc acggcctcca gtagcgatca agattttagg agcacgatag
    24601 gtttcaccgt ttacttcgat cgtttgacta tcgataaaag tagcgtaacc ttttatagcc
    24661 tcgacatgat tgctatctaa tcctctttga taagcaccgt gtagaaaatc aatatatttt
    24721 tctcgtcttt ctactagacc ggcgaaatca acatttttta aatctgcttg gataccgtaa
    24781 cttgaggcat ctcgttcaat tgtttccaaa atcgtacttg cttgccacat aactttttta
    24841 ggtacacaac cgacgttgac acatgttccg cccaattcat tcgcttcaat cagcaaaact
    24901 tttgcgccat gcatccctgc tctgtttgct gaagcaatac cgccagagcc gccaccaatt
    24961 acgatgtaat catatatttg cattttgttc ctccttattg aagagatcaa tcaaaacctt
    25021 tatttaacga tcctttttct acagaaaaca agtgtaacac ttttcttgaa ttttgtatgc
    25081 gaagatgctt aaaacaggga tatatcagct ttttccttat ttacgttttc ggatcatatc
    25141 tccaggtttc accggctgtg cgactggcat agttaaaatc atcattggat ttggtgctcg
    25201 gtcaatggat tcgccttctt cattatgcat aacttcaacg gtttgactga aatgactgaa
    25261 tcctggacca taaaattcga tttcatctcc tacagaaaac aagtttctct gacgtatcgt
    25321 agcaatctgt gttgcctcat cataagataa tacttcaccg acaaatttat atactgggat
    25381 tttacgacgt tcaccgaata attgttcgtt ttcagttggt gtattgtagt aaaaacctgt
    25441 agctaattct cgttgtgcta ctttccataa ctcgtcgatc cattcttgct tgcagacata
    25501 gttttcagga tcttccatat aactgtctac agccgcttta tagacatttg acaccgtaga
    25561 tacataatgg atcgatttca ttcttccttc gatcttgaag ctgtctacgc cgttttcaat
    25621 caattctgga atatgttcga tcattgacat atccactgca ctcatcgaga attcttcttc
    25681 cacttcgcct gtatcagtta aactgtttcg ttcttgacca aaaggcatat cgaacaatcc
    25741 atacttccag cgacaagatt gagaacatcc gccacggttt gcatcccgca tcgacatatg
    25801 gttggacaat gtgcatcgac cagaataaga gatacacatg gcaccatgga taaatgcttc
    25861 gatttcggta tccgtatttt tacgaatctc tgccacttcc tccattgaaa cttcacgagc
    25921 taaaacgaca cgttccaacc cttcttcttt ccagaattcc aatgtctcaa aattagttgc
    25981 tgaagcttga gtggataaat ggatcggcag acctggggct tctgtcgcac aaatttcgat
    26041 caaagctgga tcagaaacaa tcactgcaga aataccgaca tcacgcaaac tgcggaaaaa
    26101 ttcccctgct ccatcttcat ttccttcatg ggtgaccata ttagcagcaa cgtatacctt
    26161 cgcatcatgt tcttttgcaa aagcaactcc ttcagccatt tcttcataag taaaattacc
    26221 tgcacggctt cttaaaccat atgcattccc gccaatatat actgcatctg ctccataatg
    26281 gatcgcggtt tttaactttt ccagtgttcc tgctggcgct aggatttctg gtcgtttcaa
    26341 ttttcttttt actgtcattt ctttcatcat actccttatt tgatagcaga aggatcaata
    26401 taaaagaatc ctgtatctaa ttcacggttt tcaggatgca atttactgat ttcgctagtc
    26461 attttttccg ctttttcttg atcccagcta ccttctaaaa tttgtttttt tgcttcggcg
    26521 aatagtcgag tgatgtttac gaatttttcg cttggagtaa ataatccgtc caatttccat
    26581 gtacagtaat gatgctgata aagttcattc aattcattcg agagattcag atcattgctg
    26641 gcaaaaatat gtgtgccatg actatcttca taaatagaat aatgtgtctc ttctttttta
    26701 ggttccgaga taaaaagacc tctttcacga tctttttgtt cgtcttgttt cgtatagtta
    26761 tagtaattct gtagcagtgg acgtttggat tgatggatgc atgtagcacc atacacaagg
    26821 gtttctacag gaatatcgag tttttcttcc atcgccttca tctcttcaaa aggaacttca
    26881 cgagcaagta ctgctccaac tgcgcctttt ttcgcccaga aattgatttg acgtgcgctc
    26941 gttactaatg tttcagcatc ataaatatac ggtatacgca attcaggatt ttttttcatc
    27001 gtataaataa tacctgtatc accaactgtg atcacatcta cgtttatttc ttttaaaaaa
    27061 gccaaatatt caggtacatt tttcattttt tcaggatgaa tgatcccatt tacagcaata
    27121 ttcgcttttt taccatactt gtgtgctaat tctaccaatt gtcgctgttc ttctcgtgta
    27181 aatgaagcag gcagacgcaa tccaaattgc tcttcaccga aatatagtgt atctgcacct
    27241 gcagcaagca actgttctgc ttgttcaata gtttctactg tggttatgag ttcgatcata
    27301 attttctcct cctacaactt cataatagta gcatagctct aaaaactttc caatacttct
    27361 tctatttaaa gtcggatttt tgaaaattat ttcacaaaaa atcaaaataa agttcgatat
    27421 attctgtcca aatataaaaa gagattgtga caaaaatcgg ctctataatt
//
LOCUS       DAYYGS010000035        25773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000035 DAYYGS010000000
VERSION     DAYYGS010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.035"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1835)
                     /locus_tag="ACHA44_001343"
     CDS             complement(<1..1835)
                     /locus_tag="ACHA44_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8904639.1"
                     /translation="MTQEFIYAYKKMPDDLSVNGASLVDWEDLPEINRVLNGQYRFYG
                     NYSRSGQYRSYLKKGNFIKAKVPDGSWQYFEIYNVKKNLTSVSVTARHIGFMANKNFI
                     VKSFTDNGNGSQIMTNLKNSLAFDQKFNYLSNVGTTHQFTARQVAPVEAIIGSNNGNQ
                     NLTGVTSAELDMDNYDLKLVKQIGSDNGFRIDFGINLEAIEEEIDEESIVNSLYLVGG
                     VPDNDYDEDKEPIEYGYLEIDGVTNENRRIAKRENGDCKTVDELIKWGKTLFDNDRIH
                     EPKATHTVSMVALEHTLEYGDMYQELASLHFGDVAHVRAKELDIEIKERMVEYTYFPT
                     LGKYKDIVLGNDLSLYTSAVNTQAQELKKKIDNRTETLVQNVLNATAWITGNSGGHVV
                     FRPEKAPSEILIMDTPNVANAKRVWRWNLNGLGYSDNGVNGPFGIAITSKGEIVADFI
                     KVGTINAEVFETSFNAYGDVLKLVKGTLQIWNENKKIMELTKKGMEFWNRKESIGTIG
                     TTDSAGNPFPNAVTPTPLEENSLVIRTNGDGKYILISPTAEKGFVLLGNGKAYYFGDL
                     DIQGKLTVRGKEVIPGQNGGPSGGGETPGGYPDELKTDAEKRAWR"
     gene            complement(1832..2536)
                     /locus_tag="ACHA44_001344"
     CDS             complement(1832..2536)
                     /locus_tag="ACHA44_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8904640.1"
                     /translation="MNPDEPNFIWKNRNAVIDMNCIIEAELPDIMPNKRYETYTIQGR
                     SGELTETFDDYEPFDLEIEGITIPHSKLREVKRWLTGKSQLITHNDPDKYLEAICSMD
                     KEVPFENEWGFFYTFDVTFRCQPLKRKIGEAPKIFHSSVMDIFDHGDEIAHPYLEIES
                     NGGDITIAVGDKSLTILNTLAATIVVDTQLGKAIQEGLNLFTRGDWPVLQPEWNQVKI
                     SGKFKEVRFWNRSVYL"
     gene            complement(2548..4857)
                     /locus_tag="ACHA44_001345"
     CDS             complement(2548..4857)
                     /locus_tag="ACHA44_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8904641.1"
                     /translation="MAGKESDVVLNFKTNGEVSYSKTIKEINKEMNLAAAEYKNQVSA
                     MDKDATQTEKLRAAKQKLEKQLGLAEQRSQMLREEYEKSVKETGEYSAESEKLYKQLL
                     NSETGENKLRTALEQTNDALKEQGDVSVDTAKKLQKIEETGEKVKGVGEKMSVGVTAP
                     IVAAGAAGLAAFGEVDEALDTIITKTGATGDQADRLSQSFKNVGSNTHLPLQTVGEAI
                     GEVNTQFGFMDKKLEDSTNYLLQYAEINDTDVSQSAISARQAIDAYGLEYDDLNSVLD
                     VTTKTSQNTGQSVDDLMQKAIDGAPQIKQLGLSFGEGVTLLGQFEQSGVDSSAALSSL
                     SKATVAYAKDGKSLSQGLEELQDKVKNAGSETEAINAAAEVFGTKGGPRMADAIRRGT
                     LNLEDLAKTAGESGGAVGDTFDATLDPIDQADQAMNNAKLAMADVGEAVQVSLLPFFE
                     KATSLLQEFSKWWGSLDQDTKNWIITIAGIAAAVGPVLIVLGSLMGSVTKIVGEVKSF
                     IGVWQGLAGLFGMSGGWFALAVIAIGALVTGLIWAYNNVKWFHDGVNSFFKGVSDVAV
                     EIFNFLSGYFGGFFDGVIANFNNFYNAGKRIFSGFLDFITGVFTGNWSKAWQGLVDIF
                     GGIFDGIVAVGKAPINAMIGLINGFIGGLNNIKIPKWVPGVGGKSFSISKLPYLAQGG
                     HLINGQAIVGEAGPELLTAKNGKTTVTPLSDEEKRKGISGRVSGGSIEQHIHIGKVDA
                     NNPSELDRMNRKFAKANKQAIRDLGGVPI"
     gene            complement(5052..5516)
                     /locus_tag="ACHA44_001346"
     CDS             complement(5052..5516)
                     /locus_tag="ACHA44_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904642.1"
                     /translation="MARLSDYGIHVEDLKNSAVVIIQGAEFPISFTMQTMEFIADVYG
                     GDYSQFESDMNAMLSKKEGQISSANLSPSDLKIMRALIYAMLRTGGLEEDPETIFKFL
                     GMSGEVLSAYSTCMEIFASQTFQVEDLKKSKKPQDFQKAQAKRKKNKKNRKR"
     gene            complement(5516..6142)
                     /locus_tag="ACHA44_001347"
     CDS             complement(5516..6142)
                     /locus_tag="ACHA44_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8904643.1"
                     /translation="MGKQDTFYFEGLDDILIAMMATPDSVGTAPTYSEVLRLPIATKI
                     GVKGNGTALEKWASSKMFRRVSRETKHELALDHVGIPIEVMDEIKGLVAKSGVTFSKN
                     TAREFPYFAFGFIGNIENGGKKAVWYPNTQLSNVIDEEYATAEEETKIDDVTANLVSI
                     GLKYNNVMYASFDSNRDGASLELFNKFIAQPVYDEEQWKTLVKVGGAG"
     gene            complement(6149..6514)
                     /locus_tag="ACHA44_001348"
     CDS             complement(6149..6514)
                     /locus_tag="ACHA44_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFM8904644.1"
                     /translation="MPSNKNGFSEISDYLGNLSKVDPKKLSLESLEEAANFYLKQLLP
                     NIPKSLLKKKHMRDQIKVVVENDQVKVQFEETAFYWRFAENGTTNQRAQHFASGTYEQ
                     NKEKIEEIMTRKILDLWEG"
     gene            complement(6504..6842)
                     /locus_tag="ACHA44_001349"
     CDS             complement(6504..6842)
                     /locus_tag="ACHA44_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904645.1"
                     /translation="MSISFDELKTALKSTKLAVFRDKARLGTMYPYIVYSNVSNSKKM
                     ASGKVYKKLPYYQVSFFTLGTEQDLVVIEQALQNAGIPYSDFTGIQGDENDDTVTNYY
                     TYVRCVENAK"
     gene            complement(6832..7158)
                     /locus_tag="ACHA44_001350"
     CDS             complement(6832..7158)
                     /locus_tag="ACHA44_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8904646.1"
                     /translation="MPKRQTNNLRWKAELLDIKTGTDGNDRPTTIYEFKRLIFYEELG
                     VTSQEKYLSQQAKTDVVRRIKVRWDKSITEKLSALKIDSVTYNITRIYTNPDAREMEL
                     SLAYVD"
     gene            complement(7139..7417)
                     /locus_tag="ACHA44_001351"
     CDS             complement(7139..7417)
                     /locus_tag="ACHA44_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8904647.1"
                     /translation="MEEQTKELSLEEKFKSHIHFEEGMDDSLLSFYLNMAKDYVKTAT
                     GGQQEYLILMVAGIAYEYRVSEDELDKAMNAMTPFIVQGAIQNAEETD"
     gene            complement(7419..8783)
                     /locus_tag="ACHA44_001352"
     CDS             complement(7419..8783)
                     /locus_tag="ACHA44_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8904648.1"
                     /translation="MDKELLKKMKARREQRLTELREKVESGELREADLEAVKEEIDSV
                     IDELNGIKDELGADSGTDETDDNTDDQSNSTDSDESRSGEDNDQEEDSDSEDSSENRS
                     GMITQQQRDGLLGSIKNGLEARAKMTKKQKDQQLRKAFANFVAGNISEAEARALGIEA
                     GNGSVTVPEVIASEVITYAQEENLLRKYGTVVRTSGDVKYPILVKKADANVNKKERST
                     DIAETAIQFDEILLNPAEFDALATVTKKLLKMSGVPVEDIVVEELKKAYVRKEINYMF
                     NGDDAGNENPGALAKKAVAFKKPLDLTASGAGQKLYDALIEFKNTPVTEVMKKGRFII
                     NRAALTAIEKMKTDDGFPLLRPFTQAEGGIGYQLVGYPVDWTDAADKKGEPDTPVLYF
                     GDFSAFKIQEVIGALEIQKLVEKFSGKNQIGFQIYNLLDGQLVYSPFEPAVYRYEITK
                     PVGG"
     gene            complement(8796..9365)
                     /locus_tag="ACHA44_001353"
     CDS             complement(8796..9365)
                     /locus_tag="ACHA44_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFM8904649.1"
                     /translation="MKKKEIRTIDITNLSTRSDEETHTRTISGYAAVFNSQTLLWDDL
                     SEVIVPGAFSKAISNSDVRCLFNHDWSNVLGRTKSGTLRLEEDERGLKFEVDLPDTTV
                     ARDLVKSMERGDINQCSFGFVPTEETWDYNSEPMLRTIHEVELYEVSIVPLPAYEDTE
                     AALRSRDELEKNVEERKKLIKKINQALEA"
     gene            complement(9337..10524)
                     /locus_tag="ACHA44_001354"
     CDS             complement(9337..10524)
                     /locus_tag="ACHA44_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8904650.1"
                     /translation="MEKRGYIEDIFGHYTRYGQRYVTDSSIMESSDVYELVQDISNQV
                     ALATPIVIGPDGNEVKDHHLLNILKSPNDYLTGFEFTKLETNTLLINGETFPLTDRDQ
                     LHLAYGVTTKINERLQEEFEMNGQKIPGQMIRHIKNIGTDSLKGAGIIDLARNTLEGV
                     LSAEKVLTDKYTKGGLLAFMLKLDAHINPNNSAQTKIVKAILDQLEGTQNESDHSVKM
                     IPLGKGYSIETLKSPVDDAAILNYLGVYKKDLGKFLGINVDTYQSLMKTDIEKAMMYL
                     HNKAIKPILKNKSEHYTALFFMPNSGYRVEWKINILDFVPYSTKTNIGYNIVRTGITS
                     PDNVAEMLGFPKQNTPETQAIYISNDLSRIGQKNATDDSLPTNDQDLKGGDGNEEEGN
                     SHD"
     gene            complement(10588..12315)
                     /locus_tag="ACHA44_001355"
     CDS             complement(10588..12315)
                     /locus_tag="ACHA44_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFM8904651.1"
                     /translation="MIEPGVNYADLFAKEVRKKPKKYPKTVRLAIDRWYQWKKRKDIW
                     FDVDRANEMMDWVESFIVHTKGEMVGKPFLLEPWEKFIYSWIYGWVKENEKGQIVRVT
                     REAYVQIPKKNGKTLIAVGSLGYAMYGEGALSVDCYACASDFAQAQYAAKPFAVTILN
                     NPILLEGTKIFRGPKGTVSSITYDYIHEDMAYSNKFIVQTKNIDNIEGSNPYFVLNDE
                     LHKQEKMEQYDNFKSAQISLPQPLMFNISTAGKGSSSVGMRVYREAKEVLKRDDNDSN
                     FVLIYEPNKNYDWTDRKVWEMCNPNWGISVDLSALESAFKTAQRSAHSKAEFLTKHLD
                     VFVNGADNFFEQDQVEPCLVTTQELGDLSGEPCYIGLDLLRTRDLTCVSLNFPTWDED
                     GKAVLKVKQLYFIPNEDLEFREKEDNVPYSDLAEQGFVEFCDGKMIDQDQILQYIEDC
                     MNLYDIQQVNYDPAMSDKLVEKLENLGLECVEVAQYPKVLNAPFDDVERLFYEKRIQF
                     DNPLFLYCTLNVVAITNINGQKAPSKRQSKKKIDGFVAFLCGHKETMNQMTDIDSDEL
                     DDYLSSIYR"
     gene            complement(12312..12764)
                     /locus_tag="ACHA44_001356"
     CDS             complement(12312..12764)
                     /locus_tag="ACHA44_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HFM8904652.1"
                     /translation="MTTKAQRKAIIDEKVSAEKARILEIMNLSDLYTITLDPLIESYL
                     DIFEIYQHKYLLWKEKGFPETQKFTNKSGATNQSKHPLAQQVETWADKKMKALDLLGL
                     TNKAKTGRLIAGGSTARKDEEITRPEVKPVDELAAHRNKWRKKAGAEK"
     gene            complement(12876..13256)
                     /locus_tag="ACHA44_001357"
     CDS             complement(12876..13256)
                     /locus_tag="ACHA44_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFM8904653.1"
                     /translation="MKYCQFDGCTNKIAKGIYCTEHKRSSRSRKKKQQAKSVYHHENK
                     PFYRTQAWKDMRQFIYEREGGHCQRCGQFIFGKRAHVHHIVPIKDNELLKLDPNNLML
                     LCSKCHPIVENETEDKKVFPSYFN"
     gene            complement(13253..13639)
                     /locus_tag="ACHA44_001358"
     CDS             complement(13253..13639)
                     /locus_tag="ACHA44_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HFM8904654.1"
                     /translation="MAMIKIRTSISGTEYWDSEKKRTVVVPKDQEPNFEVKEEKGILS
                     DGKSFVAVNGELIAHSSEILDSDGNTAANFDGDEATNDQSTEDTDELDNMTAKELRAY
                     AKKHGIDIPGAIRAKGDIINLIREVE"
     gene            complement(13641..13919)
                     /locus_tag="ACHA44_001359"
     CDS             complement(13641..13919)
                     /locus_tag="ACHA44_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904655.1"
                     /translation="MNDFHEAVLSIEVESSLAKVYKKAIEAENSPYRENWNGNHAHVQ
                     VENDDYRTGMNTLVISLLSHTLPNLQETIEWYERMGAKVIRTNYKGEN"
     gene            13967..14695
                     /locus_tag="ACHA44_001360"
     CDS             13967..14695
                     /locus_tag="ACHA44_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFM8904656.1"
                     /translation="MRGVLMLQEVNIKYRDRQNTETIYLPNSCPHCGNIMTPYVYFGV
                     SSYSAFDNDRIFGVLVQCINDECNKFYSLQFINTFNEDEGYSENILIKDYYRPPIKID
                     LPENIEKVSPTFVDIYTQATQAKSEQLEQIAGVGYRKALEFLIKDYAISKNSEDEDKI
                     KKMLLGKTIENYLNDFPKLQRLAKAATWIGNDETHYVRRHTNKDVHDMDAFIKSAAQF
                     IAADYDADLADQFINGNNFMPTTN"
     gene            complement(14826..15302)
                     /locus_tag="ACHA44_001361"
     CDS             complement(14826..15302)
                     /locus_tag="ACHA44_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HFM8904657.1"
                     /translation="MRFHWLKDYQELDEQILYLKWNLNKSKLELNRWVCGDLANVRIE
                     KNSRTSSLEENIQKIENELELLIEQKEEMLLLIDSFSGIDNQIVKMKYVDQMSLEDIA
                     ESVGYSSSYVRQRHAEIRKTLNFLDEYEHRQADRLKKENEIDFYNSEKYKEQLSLF"
     gene            complement(15262..15618)
                     /locus_tag="ACHA44_001362"
     CDS             complement(15262..15618)
                     /locus_tag="ACHA44_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904658.1"
                     /translation="MKKVNVIDLPVHFRGTNYFYASNLIQKKMSKDERKILANFYLVL
                     KIEQNVFSERRIVWEKQNQRLRRKNDVWNKRQSRMERLRKNKKATPFEIAAQYYSTIR
                     GGVCEISLVKRLPRTR"
     gene            complement(15596..15781)
                     /locus_tag="ACHA44_001363"
     CDS             complement(15596..15781)
                     /locus_tag="ACHA44_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904659.1"
                     /translation="MKVYILAITEGTWMFPVGSGKIYKSKTAAYKAFEKYKKENGGGT
                     NAKILVADNWHEEGERN"
     gene            complement(15782..16054)
                     /locus_tag="ACHA44_001364"
     CDS             complement(15782..16054)
                     /locus_tag="ACHA44_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH/PUA domain-containing protein"
                     /protein_id="HFM8904660.1"
                     /translation="MIHELKILPEYFEAVTSGRKQFEIRKNDRDFKVGDQLILREYKK
                     YIKHELPGLTRPTFSYTGDSYKAEITYITDYMQKSGYVVLGIKGVN"
     gene            complement(16051..16251)
                     /locus_tag="ACHA44_001365"
     CDS             complement(16051..16251)
                     /locus_tag="ACHA44_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904661.1"
                     /translation="MKVREQFKIVSDEGNVYKIKIVNINEYREADHKYLVDLYMNDKY
                     LGEGFCGDSLFDDERILKEETE"
     gene            16379..16591
                     /locus_tag="ACHA44_001366"
     CDS             16379..16591
                     /locus_tag="ACHA44_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904662.1"
                     /translation="MNINNLVKLVEQRFKESGLEQLKKDNSPELFSNFTEDDIPTLKQ
                     LKYYSDYWTQTRENELNLEKLIIKKK"
     gene            complement(16624..16818)
                     /locus_tag="ACHA44_001367"
     CDS             complement(16624..16818)
                     /locus_tag="ACHA44_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904663.1"
                     /translation="MMIQLAGIQTGKIYFSGESKSEANQWLLKTYTNNKKLRKKYPDL
                     LLKDDQIMPEPMILTRKEGS"
     gene            complement(16831..17013)
                     /locus_tag="ACHA44_001368"
     CDS             complement(16831..17013)
                     /locus_tag="ACHA44_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904664.1"
                     /translation="MRATDPVIILEEAKFIWTHEEIEQARLLFSQGVKPSKVAEIMGQ
                     KILDVGLLLLHLAEKI"
     gene            complement(17010..17150)
                     /locus_tag="ACHA44_001369"
     CDS             complement(17010..17150)
                     /locus_tag="ACHA44_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904665.1"
                     /translation="MNEIAADHELSETALIDQIELARQYFVPYADTWSKHKNDIIFPE
                     EQ"
     gene            complement(17640..18005)
                     /locus_tag="ACHA44_001370"
     CDS             complement(17640..18005)
                     /locus_tag="ACHA44_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904666.1"
                     /translation="MIDAKQVQKQKDGMLMFEAYVLPFLNQFEVLECSASGEELEYVV
                     IRETKENVQKLNEFLCTINCWDMIAPGFLCPAMGEFLEYCRLEDAGTLDLAYLVYNYL
                     NINTDHLWFGTAERKWVVR"
     gene            complement(18045..18347)
                     /locus_tag="ACHA44_001371"
     CDS             complement(18045..18347)
                     /locus_tag="ACHA44_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HFM8904667.1"
                     /translation="MDELIAKVEQWAKDKGLDQADFSKQMLKTVEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLDFAYDEIKGRTGKMVNGVFVKSSDLK"
     gene            complement(18347..18646)
                     /locus_tag="ACHA44_001372"
     CDS             complement(18347..18646)
                     /locus_tag="ACHA44_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904668.1"
                     /translation="MDKKAAMQRIIELTYSEDWQEDKKIVAEVQKLGKSMWTEKPKRR
                     TPRKIAIWHGDRILVTGTAKQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(18639..18803)
                     /locus_tag="ACHA44_001373"
     CDS             complement(18639..18803)
                     /locus_tag="ACHA44_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904669.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            complement(18809..19078)
                     /locus_tag="ACHA44_001374"
     CDS             complement(18809..19078)
                     /locus_tag="ACHA44_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904670.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFIDCVKGLQKHLNELEQV"
     gene            complement(19090..19839)
                     /locus_tag="ACHA44_001375"
     CDS             complement(19090..19839)
                     /locus_tag="ACHA44_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904671.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            complement(19836..20528)
                     /locus_tag="ACHA44_001376"
     CDS             complement(19836..20528)
                     /locus_tag="ACHA44_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative HNHc nuclease"
                     /protein_id="HFM8904672.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGGEK"
     gene            complement(20534..21205)
                     /locus_tag="ACHA44_001377"
     CDS             complement(20534..21205)
                     /locus_tag="ACHA44_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HFM8904673.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(21198..21539)
                     /locus_tag="ACHA44_001378"
     CDS             complement(21198..21539)
                     /locus_tag="ACHA44_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904674.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            complement(21717..22415)
                     /locus_tag="ACHA44_001379"
     CDS             complement(21717..22415)
                     /locus_tag="ACHA44_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HFM8904675.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            22502..22972
                     /locus_tag="ACHA44_001380"
     CDS             22502..22972
                     /locus_tag="ACHA44_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904676.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            complement(23015..23194)
                     /locus_tag="ACHA44_001381"
     CDS             complement(23015..23194)
                     /locus_tag="ACHA44_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904677.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(23181..23519)
                     /locus_tag="ACHA44_001382"
     CDS             complement(23181..23519)
                     /locus_tag="ACHA44_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HFM8904678.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(23535..23738)
                     /locus_tag="ACHA44_001383"
     CDS             complement(23535..23738)
                     /locus_tag="ACHA44_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904679.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(23753..24529)
                     /locus_tag="ACHA44_001384"
     CDS             complement(23753..24529)
                     /locus_tag="ACHA44_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFM8904680.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            24828..25148
                     /locus_tag="ACHA44_001385"
     CDS             24828..25148
                     /locus_tag="ACHA44_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8904681.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            25166..25597
                     /locus_tag="ACHA44_001386"
     CDS             25166..25597
                     /locus_tag="ACHA44_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8904682.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
BASE COUNT         7252 a         5321 c         3992 g         9208 t
ORIGIN      
        1 attctccaag ctctcttttc tgcatctgtt tttaattcat caggataccc accaggtgtt
       61 tctcctccgc cagatggacc gccattttgt cctggtatca cttctttacc tctaacagta
      121 agttttccct gaatatctaa atcaccgaaa taatatgctt ttccgtttcc taataacaca
      181 aatccttttt ctgcagttgg agaaattaaa atgtattttc catcgccatt tgtacgtatt
      241 accaaagaat tttcctcaag aggtgtaggt gttacagcat ttggaaatgg atttcctgct
      301 gaatctgttg ttccgatcgt tccaatggat tcttttctat tccagaattc catacctttt
      361 ttggttagct ccatgatttt tttgttttca ttccaaattt gaagcgtgcc ttttacaagt
      421 tttaacacat caccataagc attaaaggaa gtttcgaata cttctgcatt tattgttcct
      481 actttgatga agtcagcaac aatttctcct tttgatgtga tagcaatacc gaacgggcca
      541 tttactccgt tgtctgaata acctaagcca tttaaattcc aacgccacac tcgctttgca
      601 ttcgccacat ttggagtatc catgataaga atctctgatg gagctttttc tggacgaaaa
      661 acgacatgcc caccagaatt accagtgatc catgccgtcg cattcaacac attttgaact
      721 aacgtttctg ttcgattgtc gattttcttt ttcaattctt gtgcttgagt atttactgcc
      781 gaagtgtaca gtgataaatc attacctaaa acaatatcct tatatttccc aagcgttggg
      841 aaataagtgt attcgaccat acgctctttt atctcgatat ctagctcttt cgccctaaca
      901 tgagcgacat caccaaagtg taaagacgct agctcttgat acatgtcacc gtattcgagt
      961 gtgtgttcca acgccaccat actgactgta tgagtagctt ttggctcatg gatacgatca
     1021 ttgtcaaata aggttttgcc ccatttgatc aattcatcaa ctgttttaca atctccgttt
     1081 tcacgtttgg caatacgtcg attctcgttg gttactccat caatttctaa ataaccatat
     1141 tcgataggtt ctttgtcttc atcataatcg ttatcaggaa caccgccaac caaatacaaa
     1201 ctattgacaa ttgattcttc atcaatttct tcttcgattg cttctaaatt gatgccaaaa
     1261 tcaatccgaa aaccattatc tgatccaatc tgttttacca gtttcaaatc atagttatcc
     1321 atatcaagtt ccgcgctagt cacgccagtc aaattttgat taccgttatt tgaaccaatg
     1381 attgcttcaa ctggcgcgac ttgtcttgct gtaaattggt gcgttgtccc tacattcgaa
     1441 agatagttaa atttttgatc aaatgccaaa ctgtttttaa gattggtcat gatctgagac
     1501 ccattgccgt tatccgtaaa cgacttgaca ataaagttct tattggccat aaaaccaata
     1561 tgccgagctg ttacagaaac ggaggtcaaa ttctttttga cattataaat ttcaaaatat
     1621 tgccatgatc catctggtac cttcgctttt ataaaatttc ctttttttaa ataagagcga
     1681 tactgtccgc ttcttgaata gttaccataa aaacgatatt ggccattcag cacacgattg
     1741 atttctggca aatcttccca atcaactaac gacgctccgt tcacgctcaa atcatctggc
     1801 atttttttat aagcataaat aaattcttgc gtcacagata cacactccta ttccaaaacc
     1861 gcacttcttt aaattttccg gatattttta cctgattcca ttcaggctgc aatactggcc
     1921 aatcaccacg cgtaaataaa ttcaaacctt cttgtattgc ttttccaagc tgagtatcaa
     1981 ccacgattgt tgcagctaac gtattaagta ttgttaggct tttatcaccg acagcgatcg
     2041 ttatatctcc tccgttcgat tctatttcca gatagggatg tgcaatttcg tcaccatgat
     2101 caaaaatatc cataacacta gaatgaaaaa ttttaggtgc ttcaccgatt tttcttttga
     2161 gtggctgaca acgaaaagta acatcgaatg tataaaagaa gccccactcg ttttcgaaag
     2221 ggacttcttt atccatgctg cagatagcct ctagatactt gtctggatca ttatgtgtga
     2281 ttaattggct tttaccagtg agccatcgtt tgacttctct cagtttcgaa tgtggaatag
     2341 tgatcccttc tatttccaaa tcaaaaggtt catagtcatc aaacgtctcc gtcaattctc
     2401 cgcttcggcc ctgaatcgtg taagtttcgt atcgcttgtt cggcataata tctggaagct
     2461 ctgcctcaat gatgcaattc atatcaatta ctgcattccg atttttccaa ataaaatttg
     2521 gttcatctgg attcataaat tgtcgactca aataggaaca cctcccaaat cacgaatagc
     2581 ctgtttgttt gctttagcaa atttacgatt catacgatct aactcagatg gattgtttgc
     2641 atcgactttg ccaatgtgta tatgctgttc aatgctacct ccagaaactc ttccactgat
     2701 ccctttgcgt ttttcttcgt ctgataatgg agtaactgtg gttttaccat tcttggcagt
     2761 cagtaactca ggtccagctt caccaacaat cgcttgtcca ttgattagat gaccgccttg
     2821 tgctaaataa ggcagttttg aaatagaaaa tgattttcca cctacaccag gaacccattt
     2881 tggtatcttt atattattca atccaccaat aaatccattg atcaatccaa tcatggcatt
     2941 aataggagct ttccctacag caacaatacc atcgaaaata cctccaaaaa tatcaactag
     3001 accttgccat gcttttgacc aattccctgt aaatactcca gtgataaaat ctagaaaacc
     3061 actgaaaatt cgttttccag cattataaaa attattgaaa tttgctataa caccatcgaa
     3121 aaatccacca aaataaccac ttaaaaaatt aaatatttca acagctacat ctgaaacacc
     3181 tttgaagaaa gaattcactc catcatgaaa ccatttaacg ttattatatg cccaaattag
     3241 tccagtaact aatgcgccaa tagctataac tgctaatgca aaccagcctc ccgacattcc
     3301 aaataaacca gcaagtcctt gccaaactcc tataaaactt ttaacttccc caacgatttt
     3361 tgtaacagat cccataagtg aaccaagaac gattagtact ggtccaactg ctgccgctat
     3421 accagcaatt gttatgatcc aattctttgt atcttgatct agagaccccc accatttaga
     3481 gaattcctgt aataaggaag ttgctttttc aaaaaaaggc aaaaggctta cttggactgc
     3541 ttcacctacg tcagccattg ctaattttgc attgttcata gcttgatcgg cttgatcaat
     3601 tgggtcgaga gtagcatcga atgtgtctcc tacagctccc ccactttctc cagcagtttt
     3661 tgctaaatct tccaggttca aagtacctct acgaattgca tcggccattc taggaccgcc
     3721 tttagtcccg aatacttctg ctgctgcatt gatggcttct gtttcagaac ctgcattttt
     3781 taccttgtct tgtagttctt ctagtccttg acttaaagat tttccgtctt tcgcataagc
     3841 cactgttgct ttagataaac tacttaaagc tgcgcttgaa tcaacaccag attgctcaaa
     3901 ttgtccaagt aaagtgaccc cttcaccaaa gctcaaccct aattgtttaa tttgtggtgc
     3961 gccatcaatt gctttttgca tcaagtcgtc tacagattga ccagtattct gcgatgtttt
     4021 cgttgttaca tcaaggacag aattcaaatc atcatattct agtccataag catcaatagc
     4081 ttgtcgagca gatattgctg attgcgaaac atctgtatca ttgatttcag cgtactgtag
     4141 gagataattg gttgaatctt ccagtttttt atccatgaat ccaaattgtg tatttacctc
     4201 accaatagct tccccaaccg tttgtaaagg taaatgagta tttgaaccaa cgtttttgaa
     4261 agactgtgaa agtctatcag cttgatcacc tgttgctccg gtttttgtaa tgatggtatc
     4321 aagtgcctcg tcaacttcac caaatgctgc aagtcctgct gctcctgccg ctacaatagg
     4381 cgcggttact ccaacagaca ttttttcacc aacgcctttt actttctcac ctgtttcttc
     4441 gattttctgg agtttttttg ctgtatcaac agaaacgtca ccttgttctt taagtgcatc
     4501 gttcgtctgt tctaatgctg tacgaagttt attttctccg gtttcagaat tgagtaattg
     4561 tttataaagt ttttctgatt cagcagaata ttcaccggtt tctttgacag atttttcata
     4621 ttcctctctc agcatttgag atcgttgttc agccaatcct aattgttttt ctaatttttg
     4681 tttagccgct cgcaattttt ctgtttgagt tgcatccttg tccatcgcag acacttggtt
     4741 tttgtactcg gcagcggcta agttcatttc tttgttgatt tctttgattg ttttcgaata
     4801 actgacttcg ccattcgttt taaaattaag aacgacatca gattctttac cagccattta
     4861 tcttagcgct cctttctacc accaaggcga tttatccata gttacagatt gaggtggttc
     4921 aaaatcggtg ttttgttgta accactgtaa ataagatttg agccacaagt taggtgtaga
     4981 ctttaaaaag aaactctcac tccaattcaa tagagtgaga gcaacgtaaa tataaaaact
     5041 ccaaggagtt cctatctctt ccgattcttt ttgtttttct ttctttttgc ttgcgctttt
     5101 tgaaagtctt gtggcttctt ggattttttt aggtcttcca cctgaaatgt ctggctagca
     5161 aaaatttcca tacaggtact ataagcagac aacacctctc cactcattcc caaaaatttg
     5221 aaaatagttt ctggatcttc ctctaaacca ccagtacgca gcatggcata aatcaaggca
     5281 cgcatgattt ttagatcact aggcgataag ttagcagaag aaatttgtcc ttctttttta
     5341 gatagcatgg cgttcatatc agattcaaat tgcgaataat ctccaccata cacatctgct
     5401 ataaattcca tcgtttgcat agtaaatgaa atagggaatt ctgcgccttg aataattaca
     5461 acagcagaat tttttaagtc ttcaacgtga attccataat cagataaccg agccattaac
     5521 ccgcacctcc aactttaacc aatgttttcc actgttcttc atcgtataca ggttgagcaa
     5581 tgaatttatt aaataattct agagaagctc cgtctctatt tgaatcaaaa cttgcataca
     5641 tcacattatt gtatttcagt ccgatggaaa ccagatttgc agttacatcg tcaatttttg
     5701 tttcttcctc tgcagtggca tactcttcgt caatcacatt ggatagttgt gtattaggat
     5761 accaaactgc ttttttaccg ccattttcaa tatttccaat aaatccaaag gcgaagtacg
     5821 gaaattctcg tgcggtattt ttgctaaacg ttactccgct tttagcaact aatcctttga
     5881 tttcatccat cacttctatc ggaataccca cgtgatccaa cgcaagttca tgttttgttt
     5941 ctcgcgacac acgacgaaac attttgctgg atgcccattt ttctaatgct gttccattcc
     6001 ctttgacacc gatttttgtg gcaattggca atcgtaaaac ttcactataa gtcggtgcag
     6061 ttccaacaga atctggcgtt gccatcatcg caattaaaat gtcgtccaat ccttcaaaat
     6121 aaaaagtatc ttgttttccc aattaaaatc acccttccca taaatcaagt atttttctag
     6181 tcataatttc ttcaattttt tctttgtttt gttcatacgt accacttgca aaatgttggg
     6241 cacgttgatt tgttgtgcca ttttcggcaa agcgccagta aaaggccgtc tcttcaaatt
     6301 gaactttcac ttgatcgttt tcaactacta cttttatttg atcgcgcata tgtttctttt
     6361 ttaagagcga cttaggaata ttaggaagca actgctttag ataaaagtta gctgcttctt
     6421 ctaaagattc caacgataat tttttcggat caacttttga aaggtttcct aaataatccg
     6481 atatttcaga aaaaccattc ttattacttg gcattttcca cacacctcac atatgtgtaa
     6541 tagttggtta cggtatcgtc gttctcatca ccttgtatac ctgtaaaatc tgaatatgga
     6601 atgccagcat tttgcagcgc ttgttcaata acaactaaat cctgttctgt tccaagtgta
     6661 aaaaaagaga cttggtaata aggtaatttt ttatagactt taccggatgc catttttttg
     6721 ctgttactca catttgagta cacaatgtat ggatacatcg tccctaatct ggctttgtct
     6781 ctgaacactg ctaactttgt tgatttcagc gctgttttca attcatcaaa gctaatcgac
     6841 ataagctaaa ctcaactcca tttctcttgc atcgggattc gtataaatgc gagtaatgtt
     6901 atacgttaca gaatcaattt tgagcgcact taatttctct gtgatggatt tatcccatct
     6961 gactttaatt cgtctgacaa cgtctgtctt ggcttgctgt gataaatatt tttcttgaga
     7021 agtcacaccg agttcttcat agaatattag acgcttaaat tcgtaaattg tagttggacg
     7081 atcgtttccg tctgttcctg ttttgatgtc tagcaattcg gctttccatc tgagattatt
     7141 agtctgtctc ttcggcattt tgaatcgctc cttgcacgat aaatggcgtc atggcattca
     7201 tagctttgtc gagttcatcc tctgaaactc tatattcata ggcaatgccg gcaaccatca
     7261 aaataagata ttcttgttgg ccaccagttg ctgttttgac ataatctttt gccatattta
     7321 aataaaaaga gagcaaagaa tcatccatgc cctcttcaaa atgaatatgt gatttgaatt
     7381 tttcctctaa agacaattct ttagtttgct cttccatctt aaccaccaac tggttttgta
     7441 atttcgtagc gatatactgc cggttcaaat ggagaataaa ccaattgacc atctagcaag
     7501 ttgtaaattt ggaatccaat ttgattttta ccagagaatt tttcaacaag tttttgaatt
     7561 tccaaggcac caataacttc ttgaatttta aatgcagaaa aatcgccaaa atataaaact
     7621 ggcgtgtctg gttcaccctt tttatctgct gcatctgtcc aatccacagg atagccaact
     7681 aattggtaac caattccacc ttctgcttgt gtgaatggcc gcaacaaagg aaatccatca
     7741 tctgttttca ttttttcaat ggcagtcaaa gcagctcgat taataataaa gcgtcccttt
     7801 ttcatcactt ctgtcactgg tgtattttta aattcgatta atgcatcata taatttttgc
     7861 ccagcacctg aagcagttag atctaaaggt tttttaaatg ctacagcctt tttggctaat
     7921 gcaccaggat tttcatttcc agcgtcatca ccattgaaca tataattgat ttctttacgc
     7981 acataagctt ttttcaattc ttccacaaca atatcttcaa ctggaacacc agacattttt
     8041 aataattttt tagttactgt tgccaaagca tcgaattcgg caggattaag caaaatttca
     8101 tcaaactgaa tagctgtttc agcaatatca gttgaacgct ctttcttgtt tacattcgca
     8161 tctgctttct tcacaagaat tggatatttg acatctcctg atgttcgcac cactgttccg
     8221 tatttacgaa gtaaattttc ttcttgagca taagtaataa cttcagatgc aattacttct
     8281 gggacagtaa ctgaaccgtt gccagcttca atccctaaag ctcgagcttc tgcttcagaa
     8341 atatttccag ctacaaaatt agcaaatgct tttcgtagtt gttgatcttt ttgtttcttg
     8401 gtcatttttg cacgtgcctc caatccgttc ttaattgatc caagtaatcc atctcgttgc
     8461 tgttgagtaa tcattccaga acgattttct gaactatctt cactgtctga atcttcttct
     8521 tgatcgttgt cttcaccaga acggctttcg tctgaatccg tactattcga ttgatcatct
     8581 gtattgtcgt ctgtttcatc agttccagaa tctgcgccta attcgtcttt aattccgttc
     8641 aattcatcaa taacactgtc aatttcttct ttcacggctt ctaaatctgc ttcgcgtaat
     8701 tctcctgatt caactttttc acgtaattca gtcaatcgtt gctcacgacg tgccttcatt
     8761 tttttcaata attctttatc catgtatttt tcctcctacg cttctagcgc ttgattaatt
     8821 tttttgatta atttttttct ttcttcgacg tttttttcta attcatcacg acttcttaat
     8881 gcagcttctg tatcttcgta tgctggcaaa ggcacaatag aaacctcgta taattccact
     8941 tcatggattg ttcgaagcat tggttcagaa ttgtagtccc acgtttcttc agtcggcaca
     9001 aaaccaaaac tacattggtt gatgtctcct cgttccatag atttaactaa gtcccttgct
     9061 accgttgtgt ccggtaaatc aacttcgaat ttcaaaccac gttcatcttc ttcaaggcga
     9121 agggttccgc ttttagtacg tcctagcaca ttggaccaat cgtgattgaa caaacaacga
     9181 acatccgaat tactaattgc ctttgagaat gcaccaggaa caatcacttc gcttaagtca
     9241 tcccataaaa gtgtttgact gttgaataca gcagcgtagc cgctaatggt cctcgtatga
     9301 gtttcttcat cggaacgtgt tgaaagattg gtgatgtcaa tcgtgcgaat ttccttcttc
     9361 ttcatttcca tcacctcctt tcaagtcttg atcattcgtt ggtaaggaat catctgttgc
     9421 atttttctgg ccaatcctag ataagtcatt tgaaatatag atagcttgtg tttctggagt
     9481 attctgttta ggaaaaccaa gcatttctgc cacgttatct ggacttgtaa tcccggtacg
     9541 aacgatgttg tagccaatat ttgtttttgt tgagtaagga acaaaatcca aaatattaat
     9601 tttccattcc actctatagc cagaattagg cataaaaaaa agagcggtgt aatgttcgct
     9661 cttgtttttc aatattggtt tgattgcttt gttgtgcaga tacatcatcg ctttttcaat
     9721 atctgtcttc atcagcgatt gatacgtatc aacattgatt cctagaaatt ttcctaagtc
     9781 ttttttgtaa acacctaaat aattcaaaat tgccgcgtca tcaacaggac tttttaatgt
     9841 ctcgatggaa tatccttttc ccaaaggaat catcttaaca gaatgatcac tctcattttg
     9901 cgttccttcc agttgatcca atatagcttt gacaattttt gtttgggcgc tattatttgg
     9961 attgatgtgg gcgtctagtt taagcatgaa cgcaagtaaa ccgcctttag tatatttgtc
    10021 cgtcaaaact ttttcagcgc tcagaacgcc ttctagagtg tttcttgcaa gatcaattat
    10081 tccagcacct tttaatgaat cagttccgat gttcttaatg tgtcgaatca tttgaccagg
    10141 tattttttga ccattcattt caaattcttc ttgaagtcgt tcattgattt tagttgttac
    10201 accgtacgcc aaatgaagct gatccctatc cgttaatggg aatgtctcac cattgatcaa
    10261 taaagtattt gtttccaatt tggtaaattc gaatcctgtc aaataatcat tgggactctt
    10321 caaaatattt agtaagtgat gatctttgac ttcgttgccg tcgggaccaa taacaattgg
    10381 tgtggccaat gccacctgat tagatatgtc ttgaactaat tcataaacat cagaagattc
    10441 catgatagat gaatccgtca catatctttg accataacgt gtgtagtgtc caaaaatatc
    10501 ctcgatatat ccacgttttt ccataaaaga atagactgca ttcgataacc gatcacgtaa
    10561 tttcaatttt tttcaccgcc tttctattta tctatagatg gaacttaggt aatcatctaa
    10621 ctcatctgaa tcaatatctg tcatttgatt catcgtttcc ttatgaccac acaaaaacgc
    10681 cacgaaccca tcaatctttt tctttgattg acgtttactt ggcgcttttt gtccattgat
    10741 gttagtaatt gctacaacgt tcaaagtgca ataaaggaat aatggattat caaattgaat
    10801 tcgtttctca taaaacaacc gttcgacatc gtcaaaagga gcattcaata ctttaggata
    10861 ttgagcaact tcaacgcatt ccaatcctaa gttttctaat ttctcaacaa gtttgtcact
    10921 catcgctgga tcataattca cttgctgaat atcatataaa ttcatgcagt cctctatgta
    10981 ctgcaaaatc tgatcttgat caatcatttt tccgtcgcaa aattcaacga aaccttgttc
    11041 agccaaatca ctgtaaggaa cattatcttc tttttctcga aactctaaat cttcattcgg
    11101 aataaaataa agttgcttta ctttaagaac cgctttacca tcttcatccc atgttgggaa
    11161 gtttaaagat acacaggtca aatctcgtgt acgtaataaa tctaaaccga tataacatgg
    11221 ttcgccactt aaatcaccaa gttcttgtgt ggtaactaaa catggctcta cttgatcctg
    11281 ttcaaagaaa ttatccgcgc cgttcacaaa tacatccaag tgtttcgtta gaaattcagc
    11341 tttggaatga gctgaccgtt gcgctgtttt aaatgccgat tctaaagcag ataaatcgac
    11401 tgatattccc cagttaggat tgcacatttc ccagactttt ctatccgtcc aatcataatt
    11461 tttatttggc tcatagatta gaacaaagtt tgaatcatta tcatcacgtt tcaacacttc
    11521 ttttgcttcg cgatatacac gcattccaac agacgacgaa cctttcccag cagttgagat
    11581 attaaacatc aatggctgtg gtaaagatat ctgagcagac ttaaaattgt catactgttc
    11641 cattttctcc tgtttatgca attcatcgtt caatacaaaa tatggattgg atccctcgat
    11701 attatcaata ttcttggttt gaacaataaa tttatttgag taagccatat cttcatgaat
    11761 atagtcataa gtaatactag aaacagttcc ctttggcccc ctaaatattt tagttccttc
    11821 aagtaggatt ggattgttta agattgtaac tgcaaaaggt ttggcagcat attgcgcttg
    11881 agcaaaatca gatgcgcaag catagcaatc aactgataac gccccttctc cgtacattgc
    11941 atatcctaat gaaccgacag caatcaatgt tttaccattc ttttttggaa tctggacata
    12001 agcctcacga gtaacgcgta caatttggcc tttttcattt tctttaaccc aaccataaat
    12061 ccaagaataa ataaattttt cccagggttc taaaagaaac ggttttccca ccatttctcc
    12121 ttttgtgtga acaataaatg attctaccca atccatcatt tcattcgcgc gatctacatc
    12181 aaaccaaata tcttttcttt ttttccactg ataccagcga tctattgcta aacgaacggt
    12241 cttaggatac ttcttaggtt tctttcttac ttcttttgca aataaatcag cataatttac
    12301 accaggttca atcatttttc agcacctgcc ttcttacgcc atttatttct gtgcgctgct
    12361 agttcatcta ctggctttac ttctgggcgt gtaatttctt catcttttct tgcggtcgat
    12421 cctccagcaa ttagtctacc agtttttgct ttgtttgtta atcctaataa atctagagct
    12481 ttcatcttct tatctgccca agtttctact tgttgtgcca atggatgttt tgattgatta
    12541 gtagctcctg atttattcgt gaatttttgc gtctccggaa atcctttttc cttccacaat
    12601 aaatatttgt gttggtaaat ttcaaaaata tccaagtatg attcgattaa tggatcaaga
    12661 gtgatggtgt acaaatcaga caaattcatt atttctaaga tacgagcttt ttcagcactt
    12721 actttttcat caataatcgc tttacgttgc gctttagtcg tcatttttgt atacaccccc
    12781 ctttgttttt tgaaaaattt gacctaacga tgcgcgtgac tccccgctac cctatctccc
    12841 cacgcgaaaa aattttgaaa atgaataggg gggcttcaat tgaaatacga aggaaaaact
    12901 tttttgtctt ccgtttcgtt ttcaacaatt ggatgacatt ttgaacataa tagcatgaga
    12961 ttgtttggat caagcttaag cagttcgttg tctttgattg gtacaatgtg atggacgtgt
    13021 gctctctttc caaatatgaa ctgaccacac cgctgacaat gaccaccttc tctttcataa
    13081 ataaattgac gcatatcttt ccatgcttgc gttcgataga atggtttgtt ctcatgatga
    13141 taaacagact ttgcttgctg cttcttcttg cgtgatctgc ttgatctctt gtgttcagta
    13201 cagtagatac cctttgctat tttgttcgtg catccgtcaa actgacagta tttcattcta
    13261 cttcacgaat aagatttatg atatcgcctt ttgcacggat agcaccagga atatcaatac
    13321 catgtttctt agcatatgca cgcaattctt ttgcagtcat attgtccagt tcatccgtat
    13381 cttcagtgga ctgatcatta gtagcttcgt ctccatcaaa attagcagca gtgtttccat
    13441 cgctatcaag aatttcactg ctatgagcta tgagttcgcc attgacagca acaaatgatt
    13501 tgccatcact caaaattcct ttttcttctt ttacttcaaa atttggttct tgatctttcg
    13561 gaacaactac tgtccttttc ttttctgaat cccaatactc tgttccacta atggatgttc
    13621 taattttgat cattgccatt ttaattctct cctttgtaat ttgtacggat tacttttgcg
    13681 cccattcgct cataccattc aatcgtttcc tgcagattgg gtagagtgtg tgacaataaa
    13741 gaaataacta acgtattcat tccagttctg taatcatcat tctccacttg cacatgtgca
    13801 tgattaccat tccaattttc tctgtacgga ctattctctg cttcaattgc tttcttataa
    13861 actttagcca aacttgattc tacttcaata ctaagtaccg cttcatgaaa atcattcatt
    13921 tgtttactct cctatcagat attttgtggt atgatttata aaaaagatgc gaggtgtttt
    13981 aatgcttcaa gaagttaata ttaagtatcg tgatcgtcaa aatacagaga caatttattt
    14041 gccaaatagt tgtccgcatt gcggaaatat aatgactccg tatgtgtatt ttggggtttc
    14101 atcatatagt gcgttcgata atgatagaat ctttggcgta ttagtacagt gtataaatga
    14161 tgagtgcaac aaattttata gtttacaatt tataaatacg tttaatgaag atgaaggata
    14221 ttctgaaaat attttaatta aagattatta ccgtccgccg attaaaatag atttaccaga
    14281 aaatattgaa aaagtttctc ctacatttgt tgatatttat actcaagcca cacaagctaa
    14341 atctgagcaa ttggaacaaa ttgctggagt tggttataga aaagctctag aatttctaat
    14401 taaagattat gcaatatcta aaaattcaga ggatgaagat aaaataaaaa aaatgttatt
    14461 aggtaaaaca attgaaaatt atttgaatga ttttcctaaa ctccaaagat tagccaaagc
    14521 tgctacttgg attggtaatg atgaaacgca ctatgttcgt agacatacta ataaagatgt
    14581 tcatgatatg gatgcattta ttaaatcagc agcgcaattt atcgcggctg attatgatgc
    14641 tgatttagct gatcaattta taaatggaaa taactttatg ccaactacta attaaaagaa
    14701 taaaagagcc acagatacca attaataaaa cggttgggat atcttggctc tttcatattt
    14761 ttttgacact aatagaatat catgctgcta acaatgttta cgaacattta gtgaacagtg
    14821 taattttaga ataaagagag ttgttctttg tacttctcgc tattgtaaaa atcaatttcg
    14881 ttttcttttt tcaatcggtc agcttgtctg tgttcatact catctaaaaa gttcaatgtc
    14941 tttctaattt ctgcatgtcg ctgtctgaca tacgatgagc tatacccaac agattcagca
    15001 atatcttcta agctcatttg atcaacatat ttcatcttaa caatttgatt atcgatacca
    15061 gaaaagctat ctatcagcaa taacatttct tctttttgct cgatcaacaa ttccaattca
    15121 ttttctatct tttggatatt ctcttctaaa gaagaagttc tcgaattctt ttcgatgcgg
    15181 acgtttgcta aatcaccaca gacccatcga ttcaattcaa gcttactttt attaagattc
    15241 cactttaagt aaagaatctg ctcatctagt tcttggtaat cttttaacca atgaaatctc
    15301 acaaacgcca ccccttatgg tagaatagta ttgcgctgct atctcgaaag gggtagcttt
    15361 tttatttttt cttagccgtt ccattctgga ttgccttttg ttccaaacgt cgttttttct
    15421 tcttaatctt tgattttgtt tttcccatac aattctcctt tccgaaaaca cattttgttc
    15481 gatttttaat actaaataaa agttggccag aatttttcgt tcgtctttac tcatcttttt
    15541 ttgaataaga ttactagcat aaaaatagtt agtaccacga aaatgaacag gtaaatcaat
    15601 tacgttcacc ttcttcatgc cagttatcag ccactagaat ctttgcgtta gtgccgccac
    15661 cgttttcttt tttgtatttt tcaaatgctt tataagcagc tgtttttgat ttatagattt
    15721 ttccggaacc gactgggaac atccatgtgc cctctgtgat agctaagatg tatactttca
    15781 tttaattcac ccctttaatc cccaacacta catatccgga cttctgcatg taatcagtaa
    15841 tataagtgat ctctgctttg tatgagtcac cagtgtaact aaatgttggt ctcgttagtc
    15901 caggtaattc atgtttaatg tattttttat attcacgtag tattaactga tcaccaactt
    15961 tgaagtcacg atcattcttg cggatctcga attgtttgcg tccgctcgtc actgcttcaa
    16021 aatattctgg tagtattttt agttcgtgga tcattctgtt tcctccttca aaattcgctc
    16081 atcatcaaaa agtgaatctc cacaaaatcc ttctcccaaa tatttatcgt tcatgtataa
    16141 gtcaaccaaa tatttgtgat ctgcttctcg atattcatta atattaacga tctttatttt
    16201 gtaaacatta ccttcgtcgc tcactatctt gaattgttct ctgactttca ccaccgttcc
    16261 tcctcttttt gaaaaaattt tagtgttttt gtaagacccc ctaaatcagc ttttttattt
    16321 gatataatac ttgtatcgat taatcaaatc gaaattaaat ttaaaaaggt gatttatcat
    16381 gaatatcaac aatttagtaa aactcgttga acaacgtttt aaagaatcag gattagaaca
    16441 attaaaaaaa gataattctc cagaattatt ttctaatttt accgaagacg acattcctac
    16501 tcttaaacaa ttaaaatatt attctgatta ttggacgcaa actcgtgaga atgaattaaa
    16561 tttagaaaaa ctcattatta aaaagaaata gcaactccag cccacacggc tggtttttta
    16621 gtttcaactt ccctccttac gggtcaaaat catcggttcc ggcataatct gatcatcctt
    16681 tagcaacaaa tcaggatatt ttttccttag cttcttattg ttcgtgtaag ttttcaatag
    16741 ccattggttg gcttcgcttt tgctttctcc agaaaaataa attttccctg tttggatgcc
    16801 tgcaagttga atcatcattt tcacctcaaa tcaaattttt tctgctagat ggagcaaaag
    16861 caatccgaca tcaagaatct tttgacccat tatttcagct actttgctcg gcttaactcc
    16921 ttgagaaaag agcaagcgtg cttgctctat ctcttcgtga gtccaaataa atttggcttc
    16981 ctcaaggata attactggat cagttgctct cattgttcct ctggaaaaat gatgtcattc
    17041 ttatgcttgg accaggtatc tgcatatgga acaaagtatt gtctagctaa ttcgatttga
    17101 tcaattaaag cagtttctga taattcatgg tctgcagcaa tttcattcat actatcgcct
    17161 tcatctaaac agataagacc gttgacgtta tcgattttta catttgtgac tgcttgactt
    17221 tctccacgct cataatattc tacagttgat actgatacat cagcttcgga gtttaattgc
    17281 agaatctgtt actgccggga taatttaata ttcttttcat aattcacctt ttttcctact
    17341 gacatcgaat atttttcatg ttatgattag ccgtgaaagg agagctatat tatgtgttta
    17401 cacatgatgt ccttagctca ttcggttctt tggaagcttt tttggaaata actctcaggt
    17461 tcttgacgca aatctcattt attctttgaa acaaaaaact tccattttcc gagagctttg
    17521 gatcacttca aaagagattt gcatttatat ttctgtgacc ttgagtcgcg taaagaacct
    17581 tacagcaatg gcggcaattc cactttcagg aaaccgcctt ttttcattga tattggtggt
    17641 tagcggacta cccattttct ttcggcagtg ccaaaccaca aatgatctgt gttgatgttt
    17701 aggtagttgt ataccagata agctaaatcc aaagtgcctg catcttcaag acgacaatat
    17761 tccaaaaatt ctcccatagc tggacataaa aatcctggag ctatcatatc ccaacagtta
    17821 atagtgcaaa gaaattcgtt tagcttctga acgttttctt tggtttcacg aattacaaca
    17881 tactccaatt cttctccgct ggcagagcat tccaaaactt caaattgatt aagaaatggt
    17941 agaacatatg cttcaaacat caacattcca tctttctgtt tttgcacttg ttttgcatca
    18001 atcatgtcta tcctcctatt cagttatttc ttccattaat ggaattattt caggtcactg
    18061 gacttaacga aaacaccatt gaccatcttt ccagtccgac ctttgatttc gtcataagca
    18121 aaatccaagc actcgtataa atccatatca ttttgcattg ccaagatgat caaggttact
    18181 actacatcgc caattccgtc tcttaaacca tgttcatctt ttctagctag agaagcggca
    18241 acttccccga tttcttcgac cgtttttaac atttgcttgc tgaaatccgc ttgatccaat
    18301 cccttatctt ttgcccactg ctctactttc gcgattagtt cgtccattat ttctcctcca
    18361 catacctaaa ctgtcttcct tttgaatcaa tccataagct cctagctcta tcccaaataa
    18421 tatttttgct cagaccagta atttcagata actgtttagc ggtacccgtt actagaattc
    18481 gatcaccatg ccagattgca atctttctcg gcgttctccg tttgggcttt tcagtccaca
    18541 ttgatttacc gagcttttgg acttctgcaa ctattttttt atcttcctgc caatcttctg
    18601 aataagtcaa ttcgataatt cgctgcattg ccgctttctt atccatcccg acattctcct
    18661 ttcagtaatt tgagtacttg atcaagtgcg ctctcacgtc cgccatgaaa tgtgttgagc
    18721 cacttgtctt cgtacgacac actttgtctt aaagcttctt gatgcattaa ttcgatctgt
    18781 gctgtaaatg tctttaggtc catcttgttt acacctgctc aagttcatta agatgttttt
    18841 gcaagccttt aacacaatca atgaatagta atttggtata tgctaaattt cttaattgcg
    18901 ttccatcgat atagagtgcg aaatagtatc tgagtttact ccaactcgaa cgatcattct
    18961 taattccttc aattccagct tcttcgagtt gatcatatac gtctctcagg acgtctaatt
    19021 cctcgccctt tttataattc gctatttcat taattagttc tagataatcg atcttcattt
    19081 ttccacctct tagaatggtg cttttgattg tctattagct cgttctagcg cttttttctt
    19141 gagataggct tcttggtcga ttgcccattc aggaagcttc tctcgtcttc ctgtgcgctt
    19201 gtatccactg cttgcgttct taggttcact tttttctttc cttgcccaac ttcgaatagt
    19261 tgccaaatag tttttataag tcttaccaga tgattcacaa tactctgaca gtcgttcgat
    19321 tcgctcttgg taatcattag ggaattctgt tttgagtttc tccatctgct catctgacaa
    19381 aagaacattt ttatactctc cgtatttatg acggacgggc ttagccttcg attttttcga
    19441 aggcggtaac tctcttatat attcttttgt attattaaat gtattattaa tagatgtatt
    19501 attatctttg actttttcgt caataggggt attgcgtttt tcgtcaatag ggtattgatt
    19561 aattcgtagg tacctattga ttatttgatt ggtaccctct ttgtaaatga tttcccgatt
    19621 caagtatcca aacttaatca aatcacttac ccatcgcgat atggtctctt tattcacacc
    19681 atataaatct gcaaagtact cattgcctgc ccaacaaaag cctctttcat tacacaaggc
    19741 cgttatctct ccgtataaca acttagtatt tggtttaagt cttttgtcgt accttacgtt
    19801 ggctggtata atcgcataat aacttcgatg ttctgtcatt tttcccctcc aatatttaac
    19861 tttttgattg ttttctggtt taatttgatc cctttgattt gatatttatt tttgaaatta
    19921 atcacaccta ttttgtgctt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
    19981 tctgcatgat caacttcttt gcgctttcgt cttcctagcg ctttgtcaaa gtgatcgatg
    20041 tcagctcctg ttttgccaca gatgcagcag actctttttg tgatgcattt gtagaagtaa
    20101 tattcttgat tcgcaggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
    20161 atgaaatcta agatgatatt tgctaagaca ttggcatcac tcacagttgt attcgattcg
    20221 tctttcaggc ttattttgcg ccctgtgaca ccttcaaaac gaaagtagaa gaattccttc
    20281 cagaagtccg ttggcatgcc tgtatcgata aaaatatcgc ctatcagcgc atagatgaag
    20341 tttcgttgct gtacggtgaa acgtctagga tcaataaaac gaatttcaat gactcgatca
    20401 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcctc attgatggtt
    20461 gcgcttatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
    20521 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
    20581 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
    20641 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
    20701 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttt gtatcgattc
    20761 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
    20821 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
    20881 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
    20941 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
    21001 caagaaagtc ttgatgagtt ccagaaacaa ccctgtagtg tttgtactca ttaatagtta
    21061 ccgttgcctg tggatcattc tttttcataa tctcccacgc gtgagcccaa gataaataat
    21121 caaaatttcc ttttttcttg agaattttat ttaacttacg actaaaaagt ttttcaaagt
    21181 tcgttgtccc tttgatttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
    21241 cctggtccgt aataagagat atcaatcaag ttatctctgt cgtactcttc tagcgcatca
    21301 atcaagccat cttcgatgac gtaaatatat tcaggtttat tcgaatgctt cgatagatgg
    21361 ataagataaa catgatccca aatagttaca tggttgccta aatcatcttg atcccaagct
    21421 agttcttcat tcgtcaaaag atttcgtctg atttttcgac cacttgtttc ctcaattttc
    21481 ggcttgcccc aatcaggatc aatcaaatat tgatctagag tggaaagttc tttttccata
    21541 tgctaaaatc tccttatgat gtgttttctt tgtgactctt tgcttgccgg cggagtcact
    21601 tttttatttg ttgccatgct ttttgcttgt caatatgttg ttggcttagg atgattggtt
    21661 tatagtattt ccaccagcaa ttagcaattg ccgtccctat tcttagcgct tcagctctat
    21721 tcattgtcat caccaaaaag tctctgttgt ctgttcagtt gatcaatttc catacggatc
    21781 gcagtttctg gcaaccacat ttcaataaat gaaacagcat catcgaatct cttacgaggt
    21841 aactcgccat atcttgggat tgaaaaggta cgtttaaatt cagaccaaaa ttttgagaat
    21901 acttttttgc tgatttcttc ataagctcgg ctttcttttc cccctagaac tcccataact
    21961 ttcatatttc ctttttgctt aatttcaaac tcttgttgtc cgctaattcg catagtatct
    22021 ttaagcatgg aaacatcttt tttaacatct ttcatttctt ctaattgata gatcatcata
    22081 tcttcaattg tttgaggaac agtattcttg cgaataacat cttccatttc gttgaatgct
    22141 tcaatgtatt tttgtttgaa gtagatagct ttctttcctg taaaccccat agccagcaag
    22201 aaaaatccat ctctactaat gaagaaaact cgtcgatttc tgccatatga atctggttca
    22261 ttaccttcta caaacatctg tccaaaattg gacacatctt tttttagtgc atcaatatct
    22321 cttaaaacat gttgatgttt tttctcgaag ctttctgcca cttgtaagct cgtagtcaca
    22381 gcttctttat ttttcaaaat tactaattct tgcattattt cttctctcct ttttggtata
    22441 atttagttaa aaactggtgg tgtttatttt gaattttttt cacttaacaa tttttcaatg
    22501 gatgtcaata ataagtttta tattgagtac gtttttaaca gggataaaaa taaaaggaat
    22561 gcgccctcaa ctagaagttg aacttaatgc ttcttacttc gctgcagata tgatttatac
    22621 aaaagtaatc atttctaact attcaacaga accagctatg ttagttaatt tagaactttc
    22681 atccgctaac ttaaaccgca gatggtcagc tactccattt aagaaattaa ttgctaaagg
    22741 aggatcagtc gatgataata gaatatactc tgaagctgta ccgctaaata ttcctcctaa
    22801 aagtgctata tcttgttacc ttgcatttga agtagggaaa attaatttta ataaactcct
    22861 taatcaccaa acaaaaatga tatttactct aaatagaacc caaatccaca aaattgttga
    22921 tatcaaaaat acaaacttcc caatagagaa attagtgaaa gaattaaact gagtaatagt
    22981 aatatacatt ctagtttctc ttttctattc atttttagtc agccccctcg gttggctttt
    23041 tcgctctgta ctcagcttca gcttcatcaa gccccataaa aatccaaacc atgtaaacaa
    23101 tcgttcctat caacgcttgc ctgcttcccc aaagtcctaa agcgtagatg attagcggtg
    23161 cgctgaacac tagcgctcta ttaaattttc ccatccgctt gcctccttaa gatttccgaa
    23221 aaatttgttt ctaaaaattc aagtgtttta cttcttaaaa atagatatgt gtctcttcct
    23281 tcaactggat aatagacaaa tccatttttg tttttctcga tatcgataat atttctatat
    23341 cttgggttct ttaaaactct agaagtaaac caatcatatt ttctgttaat ccgttctagc
    23401 acttctggca acgtcatcca tctaccagta tcatcagctt tttttaactc ctcataatcc
    23461 acttgggaga taattacata gccttctgga attgggattt ttgcttctag atattgcatt
    23521 agctgttcct ccctttacaa ttcgtacata gtgataatcg aatctataat tctgtttgct
    23581 tctgcagaag tctttttacc gtttaaaatt aaagataagt agcttttgct aattccaaat
    23641 ctttcagcaa gcatggtgta agttaagaac tttgaacttt cgacatattc tttgattttt
    23701 tctctatctc gttgagtgat ttctgcaatg tcagtcatac taaaactcct ttctaaccaa
    23761 tttcctctaa atccatttga gggtaatatc cttctttttt tagtaattcg tagataaata
    23821 gacgcccttt ctgtgtccat ttggtattca ttacaatttt agtgccacca tcggctttcg
    23881 ggatctcagt tgtatgagat tttgtgtatc cttggttcat gtgttttttg cacaataacc
    23941 attggttacc gacttttttc tgaataccta gtttatgaag taatttattc atctgttgtg
    24001 gagacatccc ataatctgct gcaatctgac taattgttac tgaatctgta gaagataata
    24061 tgctatctaa ataggagatt ttaggttcgt actctgcaat cttttgttct gcgattaatc
    24121 ttccagttct ttcttctttt agttgagttg ctaattgaat gattgtatct ggattaagca
    24181 aagcttcttc tactttttct ggagttagat aacctccatg ttttctaatt gctggcaaca
    24241 cttcacttgt tacccaacgt ttaaattttt tggcagaggg aagttttgat tttaagatta
    24301 aactgtacaa accagattcg ttgatgattg tcatctctct tgattgacct gaggtcgtga
    24361 ttcgcgaccc catcttatct tccaaatcta catgccgcga caaagcatct ttagtgtttg
    24421 aataacccaa aacactcgca acgtctttgc ctacaaaata tggttcatca tttactaaaa
    24481 ttgtccgaac ttcgttttgt tcgaaattaa aaatttgcgg tgtgttcata tttctcattc
    24541 ctttctttta tattcgtaaa caaatttaac aacttttttc taaattcaat tgacagtatc
    24601 tgagtaatac tctatactaa gagcataatt aaataagaca taaaaacatt gattttaaag
    24661 ctttcttggc ggtctgcatt tacatatcaa tagtgttttt tgttgtcttt ttagttgtta
    24721 aatctgttta caagatatag tatagctcat tactcagaat tagtcaaatt atttctgcgt
    24781 atttttctaa actttttttg taaacattta gaaaggttgt tttatcgatg aatacctatg
    24841 aaataataaa agacttggcg aagaggaaaa agatatctat acggcagtta gaaattaatt
    24901 ttggatattc taacggttat ctagcaaagt ggaaaaataa tactcctaat tcagatgaat
    24961 taagtcggat tgctgactat ttcgatgtct ctgtagacta ccttctaggt agagaagaaa
    25021 gagaaacccc taaacatgtg gatttatcag aagacgatac tgttttttct tttgatggaa
    25081 aagaaatatc taaggagaca atgcgtaaag cgattgcaat tgctaaagct ttagaggaaa
    25141 atgaatagtt ggagtgatgg tttgtatgta tttgaaattg aaagaaatgt tgagcgaata
    25201 caatttgaaa gtagtttata tggagatgaa agagcctggt ttctactatc caaaacccag
    25261 aatcattttt ttgaatgaaa atctatacgg tgaaactgca gaagcctttc atttatctca
    25321 tgaacttgca catttcagcg cctctcattt tgaattttca gttttgtacg atacttctac
    25381 aacttttcac tcaaaatttg aaacagaagc agataaaata gctattctaa ttttattaaa
    25441 tatctatatt gagaacgaac tgactgatga atctcagttt aatctcgaaa aatttatgga
    25501 gtattattct attcagaata agctcagata cacttgttat gctgtctgcc aatgctattt
    25561 taagaaaaaa tattcttacg caaggcaata tgtatgaata catcaagaat tattaaaaaa
    25621 accgatttca gcaaaacatt aaaaaagccc gtgctgcaac acgggctctt tcctcatata
    25681 tgagcttcta caaaaaaaat catatcacag aaatgaggaa gaaaaaatgg aaaaagagat
    25741 tttaataaaa tctccaggaa aaaccttgat taa
//
LOCUS       DAYYGS010000036        25440 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000036 DAYYGS010000000
VERSION     DAYYGS010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.036"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            89..643
                     /locus_tag="ACHA44_001387"
     CDS             89..643
                     /locus_tag="ACHA44_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8904683.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(816..1253)
                     /locus_tag="ACHA44_001388"
     CDS             complement(816..1253)
                     /locus_tag="ACHA44_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8904684.1"
                     /translation="MFSSEENKPLYLDYVNHNLKIIIKENNLKHITLHGFRHTHCSLL
                     FESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFMSSTEEKQGSLQK
                     IKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1301..2248
                     /locus_tag="ACHA44_001389"
                     /pseudo
     CDS             1301..2248
                     /locus_tag="ACHA44_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            2519..2954
                     /locus_tag="ACHA44_001390"
                     /pseudo
     CDS             2519..2954
                     /locus_tag="ACHA44_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345965.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
     gene            2970..3803
                     /locus_tag="ACHA44_001391"
     CDS             2970..3803
                     /locus_tag="ACHA44_001391"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8904685.1"
                     /translation="MLYFSAIDNADFVFSDPFTAEVLSGIEEQAKNHGYFVLVHNVNS
                     AEDVKTIQRNWRFEGFIVVGVRVKEFLELNNVFDGPVVYIDTHLSKETLRELEQQMNR
                     LFVNTNDYQASTLAVEHLIHYGHEKIAFLSFDYIVGEPSVIQERHQAYIDTLKKYHLT
                     FKSHWIYNNSEFERIYQELAEFTAIVVTGDYLAAKLVKFLKNKNKRVMEQLSIIGFDD
                     ITFAELMDPALTTIRLNPGNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKI
                     K"
     gene            4048..5073
                     /locus_tag="ACHA44_001392"
     CDS             4048..5073
                     /locus_tag="ACHA44_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8904686.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5211..5336
                     /locus_tag="ACHA44_001393"
     CDS             5211..5336
                     /locus_tag="ACHA44_001393"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904687.1"
                     /translation="MKHFKKIALTGIAVLTGTLLAACGGGDSNSKDGQVTLSYAS"
     gene            5361..6476
                     /locus_tag="ACHA44_001394"
                     /pseudo
     CDS             5361..6476
                     /locus_tag="ACHA44_001394"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
     gene            6559..7455
                     /locus_tag="ACHA44_001395"
                     /pseudo
     CDS             6559..7455
                     /locus_tag="ACHA44_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338086.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            7471..8295
                     /locus_tag="ACHA44_001396"
     CDS             7471..8295
                     /locus_tag="ACHA44_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904688.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8327..9637
                     /locus_tag="ACHA44_001397"
     CDS             8327..9637
                     /locus_tag="ACHA44_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HFM8904689.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9785..11044
                     /locus_tag="ACHA44_001398"
     CDS             9785..11044
                     /locus_tag="ACHA44_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8904690.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11054..11926
                     /locus_tag="ACHA44_001399"
     CDS             11054..11926
                     /locus_tag="ACHA44_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904691.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11938..12768
                     /locus_tag="ACHA44_001400"
     CDS             11938..12768
                     /locus_tag="ACHA44_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8904692.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12808..14991
                     /locus_tag="ACHA44_001401"
     CDS             12808..14991
                     /locus_tag="ACHA44_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8904693.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15052..16272
                     /locus_tag="ACHA44_001402"
     CDS             15052..16272
                     /locus_tag="ACHA44_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8904694.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16269..17729
                     /gene="gtfA"
                     /locus_tag="ACHA44_001403"
     CDS             16269..17729
                     /gene="gtfA"
                     /locus_tag="ACHA44_001403"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HFM8904695.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17806..18912
                     /locus_tag="ACHA44_001404"
                     /pseudo
     CDS             17806..18912
                     /locus_tag="ACHA44_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19234..19443
                     /locus_tag="ACHA44_001405"
     CDS             19234..19443
                     /locus_tag="ACHA44_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8904696.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMASKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19436..19585
                     /locus_tag="ACHA44_001406"
     CDS             19436..19585
                     /locus_tag="ACHA44_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFM8904697.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19739..20404
                     /locus_tag="ACHA44_001407"
     CDS             19739..20404
                     /locus_tag="ACHA44_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8904698.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20688..21773)
                     /gene="xerS"
                     /locus_tag="ACHA44_001408"
     CDS             complement(20688..21773)
                     /gene="xerS"
                     /locus_tag="ACHA44_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HFM8904699.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22107..22340
                     /locus_tag="ACHA44_001409"
     CDS             22107..22340
                     /locus_tag="ACHA44_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HFM8904700.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22337..22744
                     /locus_tag="ACHA44_001410"
     CDS             22337..22744
                     /locus_tag="ACHA44_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8904701.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(23881..24399)
                     /locus_tag="ACHA44_001411"
     CDS             complement(23881..24399)
                     /locus_tag="ACHA44_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HFM8904702.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            24766..25014
                     /locus_tag="ACHA44_001412"
     CDS             24766..25014
                     /locus_tag="ACHA44_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904703.1"
                     /translation="MVKLKMLYIGLQHRSFFDCALGKKYYEVKIDDDTFLTAHQMKQL
                     QKRNDQLEENLILKKRLPYSRLARRTIKGQDLPEDHPF"
BASE COUNT         8656 a         3889 c         4749 g         8146 t
ORIGIN      
        1 ttcaaaagtt tatatatata agttaatgat tatggtaaaa tatatacata atcattaact
       61 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
      121 tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg aaagagatag
      181 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
      241 agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa caggtaagcg
      301 aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg aaggtaaaga
      361 agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa gggatcgtgt
      421 ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag gaacacatac
      481 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
      541 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
      601 tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa atttttataa
      661 acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt cataaagttc
      721 gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat aaactaagta
      781 attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat caatacggtc
      841 tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt tagcagacta
      901 tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgatctt ctgcaaactt
      961 ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt ttcctctgtc
     1021 tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc tgtatgtcca
     1081 agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact acaatgggta
     1141 tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat gattttcaaa
     1201 ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa caaaagttgt
     1261 tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac gtattcatca
     1321 catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata atatactcgg
     1381 tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc acctgttttt
     1441 ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac aagaagggaa
     1501 caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc cttccgaagc
     1561 atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt atggaatcga
     1621 atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac gattagcttt
     1681 agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca cagaccagat
     1741 ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac aatattccga
     1801 tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg tcagtttctt
     1861 ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct atcaagaagt
     1921 tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta gcgtccatca
     1981 tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac aattacaaga
     2041 gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac tttattatcg
     2101 tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag atgcaaaagc
     2161 gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc agtacttaaa
     2221 tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg aagagtaaga
     2281 ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta aagaatgttg
     2341 atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac tacttatgag
     2401 tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa acatctgcca
     2461 aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg agtaccaaat
     2521 gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc agtctcaaaa
     2581 ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa tcatcgattg
     2641 tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat gatagtttta
     2701 cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat aatctttcta
     2761 cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg ttagggacga
     2821 ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca cctagtttag
     2881 gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc tgtcagggaa
     2941 tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt gataatgctg
     3001 attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag gaacaggcaa
     3061 agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat gttaaaacta
     3121 ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta aaagaatttt
     3181 tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat ctttcaaaag
     3241 agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca aatgattacc
     3301 aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa atagcgtttc
     3361 tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat caagcttata
     3421 tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat aacaactctg
     3481 aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt actggagatt
     3541 atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg atggaacaat
     3601 tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct gcgttgacaa
     3661 caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg attggactgg
     3721 ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg atttctcgag
     3781 actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta acaataaaat
     3841 atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa attcactaga
     3901 attagcatta tttactctaa attgttcgga tattttttct tgtttttaat tatgattaca
     3961 gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg ttagtaaaca
     4021 ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc aaaacgggcg
     4081 ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact ttcagtaacc
     4141 gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt caaaaaaagt
     4201 cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga agtagatgag
     4261 ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc agaacaaagc
     4321 aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca agacgatttg
     4381 ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat tgaagaagta
     4441 tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc tgaagtagat
     4501 gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt gtataaaaaa
     4561 ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag ccaaggaaat
     4621 aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat gaaacaaaaa
     4681 ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga cggaatgcag
     4741 gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc tatcatggta
     4801 ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg tctacatatt
     4861 ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt tttaacacct
     4921 gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt tcgcatggtt
     4981 aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc taataatatt
     5041 attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa aaagggtgtt
     5101 tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt
     5161 tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt
     5221 tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg
     5281 gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata
     5341 ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata
     5401 tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa
     5461 caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata
     5521 tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg
     5581 gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc
     5641 ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg
     5701 tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa
     5761 cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag actggctact
     5821 ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt
     5881 tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc
     5941 atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag
     6001 gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact
     6061 atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta
     6121 caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac
     6181 taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa
     6241 tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga
     6301 gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac
     6361 gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg
     6421 aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta
     6481 gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat
     6541 accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg
     6601 atatggggct atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg
     6661 ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa
     6721 tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta
     6781 ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg
     6841 gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt
     6901 ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat
     6961 gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta
     7021 acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt
     7081 tataacatgg tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca
     7141 gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt accattagtt
     7201 acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat
     7261 gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc
     7321 tatttattct ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt
     7381 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
     7441 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
     7501 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
     7561 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
     7621 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
     7681 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
     7741 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
     7801 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
     7861 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
     7921 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
     7981 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
     8041 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
     8101 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
     8161 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
     8221 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
     8281 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
     8341 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
     8401 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
     8461 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
     8521 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
     8581 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
     8641 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
     8701 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
     8761 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
     8821 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
     8881 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
     8941 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
     9001 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
     9061 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
     9121 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
     9181 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
     9241 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
     9301 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
     9361 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
     9421 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
     9481 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
     9541 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
     9601 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
     9661 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
     9721 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
     9781 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
     9841 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
     9901 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
     9961 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
    10021 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
    10081 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
    10141 tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt
    10201 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
    10261 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
    10321 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
    10381 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
    10441 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
    10501 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
    10561 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
    10621 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
    10681 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
    10741 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
    10801 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
    10861 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
    10921 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
    10981 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
    11041 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
    11101 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
    11161 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
    11221 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
    11281 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
    11341 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
    11401 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
    11461 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
    11521 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
    11581 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
    11641 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
    11701 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
    11761 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
    11821 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
    11881 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
    11941 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
    12001 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
    12061 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
    12121 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
    12181 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
    12241 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
    12301 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
    12361 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
    12421 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
    12481 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
    12541 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
    12601 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
    12661 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
    12721 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
    12781 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
    12841 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
    12901 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
    12961 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
    13021 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
    13081 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
    13141 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
    13201 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
    13261 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
    13321 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
    13381 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
    13441 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
    13501 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
    13561 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
    13621 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
    13681 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
    13741 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
    13801 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
    13861 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
    13921 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
    13981 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
    14041 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
    14101 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
    14161 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
    14221 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
    14281 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
    14341 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
    14401 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
    14461 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
    14521 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
    14581 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
    14641 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
    14701 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
    14761 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
    14821 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
    14881 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
    14941 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
    15001 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
    15061 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
    15121 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
    15181 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
    15241 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
    15301 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
    15361 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
    15421 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
    15481 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
    15541 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
    15601 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
    15661 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
    15721 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
    15781 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
    15841 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
    15901 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
    15961 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
    16021 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
    16081 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
    16141 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
    16201 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
    16261 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
    16321 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
    16381 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
    16441 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
    16501 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
    16561 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
    16621 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
    16681 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
    16741 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
    16801 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
    16861 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
    16921 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
    16981 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
    17041 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
    17101 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
    17161 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
    17221 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
    17281 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
    17341 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    17401 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    17461 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    17521 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    17581 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    17641 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    17701 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    17761 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    17821 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    17881 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    17941 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    18001 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    18061 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    18121 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    18181 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    18241 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    18301 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    18361 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    18421 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    18481 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    18541 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    18601 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    18661 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    18721 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    18781 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    18841 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    18901 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    18961 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    19021 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    19081 tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta
    19141 taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct
    19201 tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta
    19261 atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt
    19321 atggcttcaa aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa
    19381 tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt
    19441 taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa
    19501 acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa
    19561 aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta
    19621 tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg aaagtagaag
    19681 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
    19741 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
    19801 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
    19861 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
    19921 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
    19981 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
    20041 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
    20101 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
    20161 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
    20221 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
    20281 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
    20341 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
    20401 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
    20461 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
    20521 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
    20581 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
    20641 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
    20701 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
    20761 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
    20821 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
    20881 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
    20941 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
    21001 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
    21061 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
    21121 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
    21181 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
    21241 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
    21301 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
    21361 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
    21421 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
    21481 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
    21541 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
    21601 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
    21661 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
    21721 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
    21781 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
    21841 tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa gcatataaac
    21901 aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg aaatagaagg
    21961 gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca atctttctat
    22021 agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat atactgtata
    22081 tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt agggaagtgg
    22141 ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg ttaaatcccg
    22201 gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa aaacaagcct
    22261 tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat actactttta
    22321 gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta taaaaatgaa
    22381 tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg atgcgaatgc
    22441 tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg tttatccttc
    22501 gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc cttttcacaa
    22561 tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca atggacagct
    22621 tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta cgtatgataa
    22681 ggatttcgat caactaaaat atgaagtgat caacactata aagacacata cgcagcccat
    22741 ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga aataattgaa
    22801 gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa ttttctatat
    22861 agttggatta agataacgac tatttatttt atcatatttt tactataccc tatactttta
    22921 cttggtatat ttatgaataa tcgcaaattt acccctatac tactagtttg taagttagat
    22981 taataaaaat actaaagtta tttgttcttt tattaaatac acctacaact tataaattga
    23041 tggtatttag ataacacgaa agaataaaaa taacagctga tagtaaagtt tgttgacgat
    23101 tgctactcaa tcgaaagtga gttagtgcgt atgaacttgc ttctgaaaac ttatgaaatg
    23161 aaagaccttt tacatcttgc agaaatcgtt actgggatta aactaagcaa taaaaactaa
    23221 tgttcccctc attctttgaa cttctactat ttgatttatt tattctgata aatgacgatt
    23281 tcaccttcag gatcttcaaa tcccgtatag aatttattta agatctcttc agtatcagta
    23341 ataggatagg cgataggcca catacccttt gatggttaga ataaactgct caaaatctgt
    23401 atcgtatgtt ggctttagca tattaacaaa ggctatctcc aaattataaa ccataagcgt
    23461 tgaatcgttc ttaacaattt caattcttcc taacccaaag tcagaagata taattaccgg
    23521 acttgcctta cccttcatgt gggttgttga gattccatat ttaaagggaa ttgtcggtac
    23581 tatcggcggt ctgaaaaaca tcccaaaaat aaaaaaatct ttcccctgct agtctcctaa
    23641 gtccctttgt agcggatatg gcaccataat ctcattttat cgaagtgagt catggtttat
    23701 ctgttggcca accaaaattg atacaatcta acgatgcacc ctctacggca ttctagggga
    23761 gtgcacttgc attcacccaa taaaaaccgg cagttagcaa agaacttatg tagtcgtatg
    23821 ttcttactgt taacatacaa gaggtctcct ttccacaatt gcttagcagt aaacgggaat
    23881 ttacaaagct aatctcttcc attttcttat ctttgttcta aaagtcccaa atggagcaac
    23941 tgtatttaca tgaataaatt tatatacttc ccatacagcc gttttggttg cctcgtctgc
    24001 ccattttctt ttgtgtgcag tgaatagatc ttcatcactc attgaatcaa tcatcatgtt
    24061 aatatcatca atattacgat tgagaatttc ctctaattct ttcaatggta gatgtgcata
    24121 ggtatttgta aaccacttat ataactctcc taactgattc catttaaatt gatcagaagg
    24181 cgttttaaca gataatccat ttctttcatc attttcccat tttagaatta aattcgtcca
    24241 accaacttga tatgctaaat tttcagcagg tgttctatcc acttcctcaa ctcttttatc
    24301 cttcaattct tcaggaatag tttcaaattc taaaatatat ttagtataag tcctatttat
    24361 ttcttttttt aattcttctt tatttttata tgttctcatt atattacctc cataaattcc
    24421 taattgtctc ctttaattca tctataattt catccacgat attttaatcc gttctacaat
    24481 gcatatctga tatgcattga ctgtctaact catatgtgaa tgatttataa aatatcccaa
    24541 cgtctcatct aaagaaatgt tgatcgtcag caaggagcta aaaaatacga atatatttct
    24601 tttgtagtaa aatcggactg gtttgcatat cattttcttc gtaaatatca aaatcatgtt
    24661 aactactatc aattactaat aaaaactacc actttaagcg ttaagaactg ttaagtttag
    24721 ttaagtgagg atagttcaca aaatccattg ttaaacttca cgaaagtggt aaaactcaaa
    24781 atgctttata taggattaca gcatcgctct ttcttcgatt gcgcgttggg taaaaaatat
    24841 tatgaagtaa aaattgacga tgacacgttc cttacggctc atcagatgaa acagctacaa
    24901 aaacgaaatg atcaactcga agaaaattta attctaaaaa agcgattgcc atattcacgc
    24961 ctcgctcgac gaactattaa aggtcaagat ttacctgaag atcatccttt ttaggaaatt
    25021 gaagcttttt atgttgcaca atgcatttat aatactacaa tgatgttttc accagatatt
    25081 cttctagggt gcggtgtaat gaaacagata cacttgcaaa agaaaattca ccgcgaattt
    25141 actaaaatta tgaataatta tgttaatctt ccgaaatttt tttccaaaat taagggatca
    25201 agctggtgtt atagattgtt taaggttggc aaaaaaataa cttcttttta aacaattata
    25261 gaaaaccgat actctgttaa gttggttgac acggtgtcga tttttatgac tttcttattc
    25321 tgaaacttca ctaacggaca gtacctaaaa agtctatagt atggacgttt acgagtacta
    25381 ttttatcaaa aatgctgctc atttcaactt atatgtttta gtgaactacc cccacttagc
//
LOCUS       DAYYGS010000037        25137 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000037 DAYYGS010000000
VERSION     DAYYGS010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.037"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(53..787)
                     /locus_tag="ACHA44_001413"
     CDS             complement(53..787)
                     /locus_tag="ACHA44_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HFM8904704.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="ACHA44_001414"
     CDS             1114..1524
                     /locus_tag="ACHA44_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFM8904705.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="ACHA44_001415"
     CDS             1517..2218
                     /locus_tag="ACHA44_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFM8904706.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="ACHA44_001416"
     CDS             2218..3804
                     /locus_tag="ACHA44_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904707.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="ACHA44_001417"
     CDS             3823..4044
                     /locus_tag="ACHA44_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HFM8904708.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="ACHA44_001418"
     CDS             4041..4802
                     /locus_tag="ACHA44_001418"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HFM8904709.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="ACHA44_001419"
     CDS             5182..5691
                     /locus_tag="ACHA44_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HFM8904710.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="ACHA44_001420"
     CDS             complement(5766..6305)
                     /locus_tag="ACHA44_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HFM8904711.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHA44_001421"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="ACHA44_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFM8904712.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="ACHA44_001422"
     CDS             7318..8169
                     /locus_tag="ACHA44_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFM8904713.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="ACHA44_001423"
     CDS             8197..8832
                     /locus_tag="ACHA44_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HFM8904714.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="ACHA44_001424"
     CDS             8852..9625
                     /locus_tag="ACHA44_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8904715.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="ACHA44_001425"
     CDS             10033..10830
                     /locus_tag="ACHA44_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HFM8904716.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="ACHA44_001426"
     CDS             10808..11221
                     /locus_tag="ACHA44_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HFM8904717.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="ACHA44_001427"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="ACHA44_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8904718.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="ACHA44_001428"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="ACHA44_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFM8904719.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /locus_tag="ACHA44_001429"
     CDS             15998..16558
                     /locus_tag="ACHA44_001429"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFM8904720.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHA44_001430"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="ACHA44_001430"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HFM8904721.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="ACHA44_001431"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="ACHA44_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HFM8904722.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="ACHA44_001432"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="ACHA44_001432"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFM8904723.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGVFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHA44_001433"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="ACHA44_001433"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFM8904724.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHA44_001434"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="ACHA44_001434"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFM8904725.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="ACHA44_001435"
     CDS             21905..22483
                     /locus_tag="ACHA44_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904726.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="ACHA44_001436"
     CDS             22535..23017
                     /locus_tag="ACHA44_001436"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFM8904727.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="ACHA44_001437"
     CDS             23134..23508
                     /locus_tag="ACHA44_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904728.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23938..25116
                     /locus_tag="ACHA44_001438"
     CDS             23938..25116
                     /locus_tag="ACHA44_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HFM8904729.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKYQHCCIHVSRNIAHKVRVKDRKEICDDFKAV
                     YQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQDLLTFYDFPPSIRRTIYSTNLIE
                     SFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLVSMFTE
                     "
BASE COUNT         8267 a         4524 c         5333 g         7013 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggtat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttttt gagagtataa aatattttgt gtaaatgaaa aaatccatac aaaaaaggaa
    23881 gtcccttctg tagaataaag ttaacgacaa ccaattcaca gaaaagagga cttccctatg
    23941 aatgatttta ctacagaaat tgtgcaaact ctagtcacta aaggcgattt aaatgaatta
    24001 ttccgttcgc acttagaaaa agcgataaac acactcctac ggactgaatt aacggctttt
    24061 ttagattacg aaaaatatga tcgcactggt tttaattcag gtaattcgag aaacggttct
    24121 tactttcgat caatcaaaac cgaatatggt gaattaacat tggaaatacc tagagatcgt
    24181 aatggtgagt ttaaacaaca aactttacca gcctacaaaa gaacaaacga tacattggaa
    24241 accactatta tccatttatt cgaaaaaggt gttacgatgt ctgaaattgc tgatttgatc
    24301 gaaaaaatgt acggtcatca ctatactcca caaaccatgt ccaacatgac taaagttctg
    24361 actgaagaag taaatgcctt taaatccaga gccttaaatg ataagtatgt cgctattttt
    24421 atggacgcta cttacattcc actaaaacgt caaaccgtat ccaaagaagc gatttatatt
    24481 gccattggta tacgagaaga cggcactaaa gaagtactga gttatgcgat tgctccaact
    24541 gaatcaacat acgtttggaa tgagctgcta caggatatta actccagagg agttcaagaa
    24601 gtcttgcttt ttattacgga cggcttaaaa ggcatgaaag atactatcca tcaaatttat
    24661 cctaaagcaa aatatcagca ttgttgtatc catgtatctc gtaacatcgc tcataaagta
    24721 cgtgtcaaag accgaaaaga aatctgtgat gactttaagg ctgtttatca agctaactca
    24781 aaagaagaag cgaatacctt cttatccggc atgattgaga aatggaagaa aaactatcct
    24841 aaagtgacgc agtcactcat agaaaaccaa gacttattaa ctttttatga ttttccacct
    24901 agcattcgta gaaccattta ctcaaccaat ctaatcgagt ctttcaataa gcaaattaaa
    24961 agatacagcc gtagaaaaga gcagtttcaa aatgaagaat cactagaacg ctttctagtc
    25021 agcatttttg atacatacaa tcaaaaattt ctaaacagaa gccataaagg ttttcaacag
    25081 gtaaccgata cattagtttc aatgtttact gagtaactaa ttattttgca ggaggac
//
LOCUS       DAYYGS010000038        24933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000038 DAYYGS010000000
VERSION     DAYYGS010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.038"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(368..1486)
                     /locus_tag="ACHA44_001439"
     CDS             complement(368..1486)
                     /locus_tag="ACHA44_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904730.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1719..1841
                     /locus_tag="ACHA44_001440"
     CDS             1719..1841
                     /locus_tag="ACHA44_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904731.1"
                     /translation="MHLIMIFSLLSIISLFAFSASRLLDAETQPIPVKVRKNDR"
     gene            1957..3264
                     /locus_tag="ACHA44_001441"
     CDS             1957..3264
                     /locus_tag="ACHA44_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFM8904732.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3383..4582
                     /locus_tag="ACHA44_001442"
     CDS             3383..4582
                     /locus_tag="ACHA44_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8904733.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4609..5877)
                     /locus_tag="ACHA44_001443"
     CDS             complement(4609..5877)
                     /locus_tag="ACHA44_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HFM8904734.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     regulatory      complement(5932..5998)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(6040..6686)
                     /locus_tag="ACHA44_001444"
                     /pseudo
     CDS             complement(6040..6686)
                     /locus_tag="ACHA44_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7094..7597)
                     /locus_tag="ACHA44_001445"
     CDS             complement(7094..7597)
                     /locus_tag="ACHA44_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8904735.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7720..8484)
                     /locus_tag="ACHA44_001446"
     CDS             complement(7720..8484)
                     /locus_tag="ACHA44_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFM8904736.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8591..8866
                     /locus_tag="ACHA44_001447"
     CDS             8591..8866
                     /locus_tag="ACHA44_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFM8904737.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9582..10535
                     /gene="yidC"
                     /locus_tag="ACHA44_001448"
     CDS             9582..10535
                     /gene="yidC"
                     /locus_tag="ACHA44_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFM8904738.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10657..11866
                     /locus_tag="ACHA44_001449"
                     /pseudo
     CDS             10657..11866
                     /locus_tag="ACHA44_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(11911..12311)
                     /locus_tag="ACHA44_001450"
                     /pseudo
     CDS             complement(11911..12311)
                     /locus_tag="ACHA44_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723846.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(12558..12992)
                     /gene="tnpA"
                     /locus_tag="ACHA44_001451"
                     /pseudo
     CDS             complement(12558..12992)
                     /gene="tnpA"
                     /locus_tag="ACHA44_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13170..13536)
                     /locus_tag="ACHA44_001452"
                     /pseudo
     CDS             complement(13170..13536)
                     /locus_tag="ACHA44_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(13550..13835)
                     /locus_tag="ACHA44_001453"
                     /pseudo
     CDS             complement(13550..13835)
                     /locus_tag="ACHA44_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13844..14035)
                     /locus_tag="ACHA44_001454"
                     /pseudo
     CDS             complement(13844..14035)
                     /locus_tag="ACHA44_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14035..14511)
                     /locus_tag="ACHA44_001455"
                     /pseudo
     CDS             complement(14035..14511)
                     /locus_tag="ACHA44_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14979..15332)
                     /locus_tag="ACHA44_001456"
     CDS             complement(14979..15332)
                     /locus_tag="ACHA44_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904739.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15460..15870)
                     /locus_tag="ACHA44_001457"
     CDS             complement(15460..15870)
                     /locus_tag="ACHA44_001457"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904740.1"
                     /translation="MEEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSN
                     LWFRQEISIAEIEDAVLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKN
                     ISYLKVYTATPPKKPTPPRFQSNGWSLFFSHKSA"
     gene            16029..16220
                     /locus_tag="ACHA44_001458"
     CDS             16029..16220
                     /locus_tag="ACHA44_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904741.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16262..17128)
                     /locus_tag="ACHA44_001459"
     CDS             complement(16262..17128)
                     /locus_tag="ACHA44_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFM8904742.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17152..18804)
                     /locus_tag="ACHA44_001460"
     CDS             complement(17152..18804)
                     /locus_tag="ACHA44_001460"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HFM8904743.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18809..19603)
                     /locus_tag="ACHA44_001461"
     CDS             complement(18809..19603)
                     /locus_tag="ACHA44_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HFM8904744.1"
                     /translation="MTLTEVLQEHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPT
                     DEYAIEKENGQLGKTEMWYVLDASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKK
                     NDVFFIEAGTIHAIGAGALIAEIQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVAN
                     LKGLSEPKQPLRVLKYRAGVAAELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVL
                     LCVNGCGTVRFDNESLDIYKGDCLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19795..21108)
                     /locus_tag="ACHA44_001462"
     CDS             complement(19795..21108)
                     /locus_tag="ACHA44_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFM8904745.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21176..22120)
                     /locus_tag="ACHA44_001463"
     CDS             complement(21176..22120)
                     /locus_tag="ACHA44_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014424865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HFM8904746.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22157..23242)
                     /locus_tag="ACHA44_001464"
     CDS             complement(22157..23242)
                     /locus_tag="ACHA44_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8904747.1"
                     /translation="MNKHICFYLSSHGFGHIARNLPIIMATLCRCEAKITLVCGANHL
                     DFAKRNLEQELPKELIDRVRYRPINTDIGLINREGTLLVDTGKLTEICEYFLAKVPSL
                     AKTEAEWLKREAVSAVLCDMPIWAMDACDMAGVPFLYVGNFTWTELYREFLPEHIWKE
                     YAKRYGKIQHTMLYALNNEEMLEFLQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVF
                     VALGMSARFKQPIDVSHAPYDFITTLGVPLVGENVTVISNSTPNTQDYIAAADYVITK
                     AGWGTVAECILAQRPMALFARDTVLEDRTTICKLQKLGLAISVTPENLHRVERVIEDL
                     KQLDTSNFDTFYDAASEIADKLISLMQ"
     gene            complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="ACHA44_001465"
     CDS             complement(23250..24296)
                     /gene="gmd"
                     /locus_tag="ACHA44_001465"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFM8904748.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
     gene            complement(24568..>24933)
                     /locus_tag="ACHA44_001466"
     CDS             complement(24568..>24933)
                     /locus_tag="ACHA44_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8904749.1"
                     /translation="QPLTEVEIFHTDRGKEFDNQAIDELSTTFDINRSLSHKGCPFDN
                     AVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNRLRLHGTLGYETPVGYRNQR
                     LAQRILDNELGCANASEAV"
BASE COUNT         7092 a         5643 c         4750 g         7448 t
ORIGIN      
        1 tttttaaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt cacaaaaatt
       61 tgagaaataa tttttgtcaa tctcttctta tttcttgaag ctgaccactt ctgtcacaac
      121 ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagcccac caaacaaaaa
      181 atatgaagag ctatttttgc ttgctgttct tattacttga tgcagaacag ctttttcaca
      241 acctctcata cacggtgttt aatcagctga ccttcggtat tagctcaaaa aacggaaaaa
      301 tatgtgaaac tatttttccg tttgtttctc taatactcag gtcaaaacac tgatttcaca
      361 acctctttta ataacgaaga atcgctgtca gactcttttc atctggcgca tctttttgat
      421 ccaaaataaa cactttcccg ccattaatga tcacatcatc tgctaatgca ttcaacactt
      481 gacgccgatc atattctgtt tcaggatctt caccgaaacc atcagctaag tttgacgtag
      541 cgataaataa atgtgacact tttccttctt tcgcagctgg aacgatatca actaactgat
      601 cgatgaattt cttatctaag agtttattgt attcttttga ttcttgagct gcgagttcac
      661 ttgacagctt cttcgcccct tcttcgatat ctctaaagga tagctgcgca ggagaagcag
      721 aaatcgcaac gtctgcacaa taatagggat ttttagcaat ttttttgaac atcgtctgat
      781 tttctggtaa tgcaaataaa taaatcggcc attgttcagg attttcgaaa gaatccttca
      841 agtacgtatc aacagcttgg taatagttga cccaatcgat ctctacttct tcatcttttg
      901 gatttacgcc atggaaagtt actccatctc gggctccgcc tcctccatta gaagagaagt
      961 tcaaaccgcc acctgtcagt tcagatccca atgcagtaac gacatcaacc ggtgcccctt
     1021 ctggtagaga tacttctttt acttgtttgt tgctgacctc atacaatttc atggagtcgc
     1081 ggttcaatcc caataaataa taagaatagt tgaactgccc atttttgata atagccagta
     1141 aataaggaag atcacttaca tagtattgat cgtctaccgg gatactcaaa cgatggatat
     1201 aggtctggtc agcagttata atgaccgcta cactcgtagt tgcactacgc caaaacgaag
     1261 gatctcctaa caaattttca attttttctt ggaacgggga ccagtcatgt tcggaatatt
     1321 ttttctcaaa acgtttcttt gcttctttcg caaaattttt gaatttgatc tgatcttttt
     1381 ccacatcttg atgggcgaca tgcgtattca gcatgatcgt gacgaatggt cctttagcgc
     1441 cttctgaagt caattgtgtc aataattctt tatccttttt tgccataaac gtacccctcc
     1501 taaaaaaatc attcctttgt ttctatctaa agtctaccac aacactattt ttccatacaa
     1561 taaaacgtat ctaaaaaaat aaaacggttt ctacggtttt aaatttagct atttttttaa
     1621 gttatcttca taatttcctc aattatttag ccccagtctt caactattct tcatgaaatg
     1681 ctgataaact aagaaaaaga ataaaagagg tggatcttat gcatttgatt atgattttca
     1741 gtttattaag tattatcagt ttatttgctt tcagcgcttc ccgcttatta gatgcggaaa
     1801 cacagcctat tcctgtaaaa gtacgtaaaa acgaccgcta aacttccata acgcgcgttc
     1861 ttatcctaaa gttttttcag aaacattgaa actctctccc ctctctgata aaattttaat
     1921 ttgtagcgaa acaaattaat tacaaggaga tcggatatgt ccataaaaaa aacacgtcta
     1981 gtcctattat tggctagtct cattctttgc cttgcttctt ttctaccagc tgttgctgtg
     2041 caagctgaag atacgttcaa agtaaatgca aaagcagctt ttgctgtaga tgcgcaaagc
     2101 ggtaagattc tttatgatca agatggagaa aaaaccatgg ggatcgcttc aatcacaaaa
     2161 atcatcggtc tttacatcgt ccttgatcaa gtaaaagaag gaaaactgtc atggggtgat
     2221 aaagtttcga tttcagatta tgcagaaaat ttgagtatca ccccagattt atcgaatgtt
     2281 cctttgcata aagaaaacac ctacactgtc aaagaattat tcgactctgc aatcatccag
     2341 tcagccaatg cttcaatggt ggccttggca gaaaaaatct ctggcagcga agctaaattc
     2401 actgaacgaa tgaaagaaca attgaaagat tggggaatca aagatgcaac aatcgttaat
     2461 gcatcaggat tgaacaactc ttatcttggc gaaaaccgac cagaaggcac tggagaaaac
     2521 gacgaaaatc aaatgtctgc tcaagatgtg gccatcgttg cccgccattt gatcttggat
     2581 ttcccagaaa tactggatgt atcaagtact acgacccaaa tgtttggcga aaatacgcaa
     2641 tcaccagtgg aaatggtcaa ctggaactgg atgctcccag gattcgttaa ttacaaggaa
     2701 ggcgtggacg gattaaaaac tggtacgact gagcttgcag gtgcttgttt cgttggtacg
     2761 atcactaaaa acgatcaacg gatcatcact gtcgtattga atgcggacgg ccatgcggaa
     2821 aacccgagtg cccgcttcaa tgaaacagac cgtttgatgg actactgcta tgacaattgg
     2881 tcagaaaaag agctcggaaa agctaacgca agtattccta gtctaaaaac cattgatgta
     2941 aaagacggaa aagaatcaac agttcctgtt gtgcttaaaa gccctgtaaa agtatgggta
     3001 cgaaacgata tggatactgg caaattaacg atcacgccaa cacttgacaa gaagcaagtc
     3061 actgacaatg agatccaagc acctatggat aaaggtacaa aaatcggtac cgctacgatc
     3121 accttggcag acgataaatt aggctattta gaagacaaca atgcaccatc aacagagatc
     3181 attacggata aaacagtgga aaaagcgaat atattcgtga ttggctggcg acgcgtcgca
     3241 gaatttttcg gcaacttgtt ttaattgaac aataagaaaa acatctttcc atcggaaaga
     3301 tgtttttttg ctttttctag tttaaataac agcttttttc atctattttc gttatactgt
     3361 ataagaaaaa ggagttgaat ttatgaatct aaccagagat ctgatctttt tagttctcta
     3421 tcttattggg gtattctcta tcgtttattg gcgaaaacag aagaaggatc cttccttgtt
     3481 cttttacggc agtacgtgga catttacggt aatcttaact tattttgtcg tgttggaaat
     3541 tttttattcg accttttatt ttttgattcc attgttgttc tttagtatct ttgctttttc
     3601 ttatttcaca gaaaaacgaa ggctactgaa tggtctttta ttcaatgttt tactcatcag
     3661 tttcggcatc tacttgtttg tcttgctgta tgaaacgcaa aatatttttc ttggcggact
     3721 gatcgccttg atcacgattc cattgctact tgtcctttta tttggtattt atggattgat
     3781 tgtttttctt ttttggaacg gggtcacagt tttacgtaga gaatcgcatt cattagctaa
     3841 tcttttaacg ttgatactag ccatttttct tacactgttt cttgtttttg actttttctt
     3901 gttaaaatat ttgccgcaat ggataaatgc tttgtttttc tgcgttcccc tgatcttgat
     3961 ctatctattt atcgtattct acaacttttt gactgtctca ttcttgtatc agttcaaccg
     4021 gcctcgatac aatcaagact tcatcgttgt gttaggcgct ggattgatca atggagaaac
     4081 agtttctcct cttctagcca agcgaatcaa taaggcaatt gctttttatc gggctcaaag
     4141 ccgtgctaca ttaaatccgc ccatcttatt gatgtctggc gggcaaggag cagacgaaaa
     4201 agtaccagag gccattgcga tgaaacaata tgccatggaa caaggaatcc ctgaaagaga
     4261 cattcttgtc gaaaccaatt cgacaaccac tttggaaaac atgctctatt caaaggaaat
     4321 catggatcag caaatgaaag ggccataccg cgcgatcttt agttccaaca attaccacat
     4381 ttttagagcc ggtctttatg cgcggcaagc caaattgaaa gcaaatggta ttggtgcaaa
     4441 aacagctttc tactacttgc ctaatgcgtt tttaagagaa tacctcgcca tcttgatgat
     4501 gaacaaaaaa agacatatga tcatttgcgg attgcttttc gcaggctccg cattcttatc
     4561 gctgattact ttacttttct aaaaaaacaa ggtgtgacaa aacatatgtc acaccttgtt
     4621 tttttctttc attctcttaa ctattggcaa aaagagtatc ccttttttga ccgctacttg
     4681 ctgagcagaa taaataccgc tgttgcctga acacataaag ctgatcaagc agatcataaa
     4741 gaagtatact gccgcttgtg aaccgaacaa ttcgatcccc atcacaaagc aggcaatcgg
     4801 tgtatttgtc gcaccagaaa atactccgat aaagcccaat cctgcaagaa aaggaacaga
     4861 aacatggagc aaaggcgcta gtgctgcacc aagcgttgca ccaatttcaa acaatggtgt
     4921 cacttctccc ccttgatatc ccgcgcctaa agacaaaact gtgaagaata acttcccaat
     4981 aaaatcatag acatgtgcct gtccattaaa tgattcttga agtaacggaa gactcaatcc
     5041 gagataacgt tgtgtttgca aaatcaatgc tgctgccaca actacagctc cacctatcgt
     5101 atttcgacaa acaggatgag gaatccattg tgcatatcgc gcttttaaaa agacgatcga
     5161 acggctgaat acccaaccaa tcaaaccaaa agcaatccca gcaacaaaaa ctttgaagaa
     5221 cagcaacata ctccattcag gaatcacacc catttggtaa tgtagatgag aaacaccata
     5281 gctttctgtc atgaaatttg caaacagtgc agcaaagaag ctagggaaga tcgcttctgt
     5341 tcggactttc ccgattgcca acacttctaa tccaaataat gttccagcta aaggtgtgcc
     5401 aaatacagaa ctaaaacccg cactgattcc actgataatc aagatttccc gttctgattt
     5461 atccaatcgg aacaaacgtg ccgtattatc tgctaacgct ccccccattt ggactgctgt
     5521 tccttcacgg ccaacagatc ccccaaataa atgggtagtt atggttccaa acagtgtaag
     5581 tggaataagc cgcaacggga ttttttcttc tccaccattt ccttgatcga tcactaagtt
     5641 gtttccacga ctagaaagtc cgccgtgata cgcatagaga taagcaaaaa gcgcaccact
     5701 aactggtagt agacaaagga gccaaggatg tgatagtcgg atatctgtca caactgtcag
     5761 gctatttaag aaaaaagcag acaagctccc catgaatata ccgagaatcg ctgtgatcac
     5821 cagccacttc agcatataca ctaaaatgcg tactggactc ggtaattgtt tttccataaa
     5881 aaattcctcc tcaataaaaa aacgcctact aaagaaaggg gctgtcccca gactttagta
     5941 ggcgtcatta gctatctttc gttagcattt aaaggcgaac accatcacct attcacttcc
     6001 attaaaagat agcatatttt tctgtaaaaa acaataaaat tatcgttctt tctttacacg
     6061 ttcagcaaat tcatagaaag gagccaattt cgttaactgt tgattacatt ggtcactatc
     6121 atgacaaata taattcccct ttttcgtata cgtgccatca gaacctgctt tcgttgtaga
     6181 aagaaaaagc gaaacattcg atgttttctg acagattgca cagattccct tatttatcgt
     6241 tggtgataaa gtaccgctaa tcccttttaa tttttcatct tcgtaataga ccatgaattt
     6301 tttctgcgta cctggatcat tccagccgat gtacgtatgt tcttgcaagt cgatttcatt
     6361 ccaagctggt tgtttgagtt ttttcacttt tcggaataat ttctcgattt gtggtttgct
     6421 aggctgcata aacggctgaa cgatttgctt cagctcctcc aatgctcttt ctgcttgcgc
     6481 ttttgtcagt tttacgtcta aaactgtcgt taaaaatgta tcgattctgg cagttcgtga
     6541 tctaatatgt ctcgaatctt ttcagctgct agtgcctgaa ctgtttgtac cgtttttgga
     6601 tcattgacag atgagtacgc atttaataac tgttctactt ctcttattat aaaaaaatat
     6661 tggtatggtt ttattgtttg ttccatgatt ttaaagtcct ttcttctaac caaagtgcat
     6721 actttgtaga aaaaagtgat cttctgcaaa gtatgtgtta cctgaagatc ctggactctt
     6781 ttaaataaga tacgttttgc atcttatcat cacccccttt aatgcttatt attctatgta
     6841 catgattcat tcgataaata aaagtaaacc gtatcttgaa cagatagtca actgcaaaca
     6901 gaaaaaagga ctgtgacaaa agtcgtgtca aagtcccttg tttcgaatga atggtgttca
     6961 aaagcttgca tctgcggtaa taagccgaaa cttcacaaaa atttgagaag taattttcgt
     7021 aaattccttc ttatttcttg aagctgacca cttctgtcac aacctcaata tggtctatta
     7081 ttggttttac ctgttaaatc gaggattctt tcccaaagta acgttgccat ctcttacgca
     7141 tacttgcaga catgggctga gccgcttctt tgactgcgca gtatttgtcc acgaatgtca
     7201 caatatatcc ttctttatac ttaggcggtg cgatcgttgc accccacatg tgcttcaaaa
     7261 tgatatctcg ctcaagatcc gacaaatcgg tgattttttc tgcattcttt acagcaattc
     7321 ttggatggat atacgcatgt gtcccctcgt caaatttcgt cgtacgccaa tcatagtaga
     7381 acagatcatg caaaagtcca gcacgcgctg ttgctttagc attgccaccc catttttttg
     7441 cgagcagata actatagtaa gaaacactga tggaatgctc caaacgagtg gaatgaacat
     7501 gctgtgtgta attcgctaat tttttcactg cttctgtttc taaaagatct tgtacataag
     7561 acatatattc ttcgtcttca cgccattttt ctgtcataat tatcgatttg ccaccttttt
     7621 gaatttcatt ttcttctgaa attgatctaa gtataccacc agtgttcgga ttattctagt
     7681 cgctctagat aaaatagcta aagcttaata gttccttaat cataaagaaa aatgataaag
     7741 aacgatccct gctgcgactc cgacattcaa tgattccgca tttcctttca ttgggatata
     7801 tacgttctga tcagtcgaag acaagatttc ctgacttact ccctgacctt cgtttcccat
     7861 gattatagcg aaatcctctg ttttttctac tttgtctaat gcatgtgctt cttcatttaa
     7921 ttctgtaccg tacaccgtaa accctttgcc tttcattaca gggatcactt ctgccaaatc
     7981 tttttgtaac acaggcaaat gataattgct tccctgcatg cttcgcagca cctttgtact
     8041 ataaatatct gccgttcctt tacctaataa aacaccagcc ataccaaacg catctgccgt
     8101 acggatcatc gttcccacat ttccaggatc ctgtacatta tctagtaaaa gccagcctcc
     8161 tgaaaaatct ggcgtatctt cagatgttaa cgtcatcact gctaagatcc cttgcggtgt
     8221 aggtagatct gacatagcgg tcatgacctc atctgaaact agaaaagtag aaatctcttt
     8281 gtttacagcc agccagtctc cccattcttt catacctctc tgtgtcacaa aaagttcttt
     8341 tatttctgca tcagacgacg cagcttcttc gatcagatgg aacccttcca aaagatattc
     8401 tgccttttct tctctgtatt tctttttatg tagttttttt aattctttga ttcttggatt
     8461 ttttactgat tggatctctt tcacattcct cacctcaact gattatatca tggaataggt
     8521 gcttgagctt gtttcatcct aaaaaaatcg gtatcattga tttataaaac aggaaagaaa
     8581 ggtgacgaac atgcgcaaag ttaaaatgaa tgtccaagga cgtgtccagg gcgtgggatt
     8641 tcgctatatg accaagatgg ttgctgatca gttaggcgtg accggaacgg taaaaaacga
     8701 agacgacggc tcagtcacaa tcgaagcaat cggaaatgac gacatcatcc aaaaatttat
     8761 cgaagaagtc aagaagtctc cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
     8821 agatccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtttttgaag
     8881 cgctttgcct cgagtattaa ggagaaacat tttccaaata gcttctcata ttttgaaatg
     8941 taatgactta ataccgagag gcagctgata aaacaccgtc aataaggttg tgaaaaagct
     9001 ggtctgcatc aagcattaag gactggttgt gaaaaatagc tctccatatt tttcaacagg
     9061 ctggacttaa tgccgtagat gcaagctttt gaacatcgtc gataaggttg cgaaaaagct
     9121 gatctgcatc aagcattaag gactggttgt gaaaaagtcg cttagttttg agtattaagg
     9181 aaaattttgt gaaaatggct ttccacattt ttgcataaat ttgacttaat accaagaaac
     9241 tgacttttga acatcgtcga taaggttgtt tttgaggcac tttgcctcaa gtattaaaga
     9301 caggtagtaa aaaagctgat ctataacttg caataaattt gcttaaccca tattttccag
     9361 acagaacaaa aaagttttta gttttttaaa taagaaagaa acacttggaa tcactctttt
     9421 ctttctaagt atttctttct tgattttttg tcttttttca aaatttttcg ctacgaaaaa
     9481 gaacgaatat ttatatgaaa tttctatgaa gttgattgaa ttccatgcgc ttttttagta
     9541 tagttaagtt tgtgtaaaaa ttatagaaga ggaagtattc tatgaataaa tggaaaaact
     9601 ggttattggg atccggactt gtcagtatcc tgctcttttt atctggctgt gtgcggatgg
     9661 attcaaacgg aaaccctgac acttcaggaa tcgtttatcg agtactcgtc catcctatgg
     9721 gccaagcgat tacatatcta gttgagaatt tcaattggtc ttacggctgg gcagttattg
     9781 caatgactgt gatcgttcgt atcatcatct tgccactagg tatcagtcaa tcgaaaaaaa
     9841 caatgatcca atctgaaaaa atgcaagcgt taaaaccaca agttgaagca gcacaacaaa
     9901 agctaaaagc tgcaacaaca cgtgaagaac aaatggcagc acaagcagaa atgcagcaag
     9961 tatatcgcga aaatggattg agtatgaccg gcggaatcgg ctgtctgcct ctattgatcc
    10021 aaatgccgat cttctctgcc ttgtatttta cggctcgcta cacagaaggt atccgtgaat
    10081 catcttttta cggcatcgat ctaggctcac caagtatggt gcttgtggcc atcgcagggg
    10141 gcgcttatct gcttcaagga tatatttcaa caatcggtat tcctgaagaa cagaaaaaga
    10201 cgatgcgtac gatgctgatc gtctctcctg ctatgatcgt cttcatgtcg atcagtgcgc
    10261 cagccggtgt tacactttac tgggtagtcg gtggtgtgtt cagctgtctg caaacattca
    10321 tcacaaacgt tataatgaaa ccgcgtttga aagcacaagt ggcagaagag ttgaaacaaa
    10381 acccgccaaa acaagtcgtt actccaagaa aagacgtgac agaatcagca gcaaagccag
    10441 ctgacaacaa acaagcaaca aaacaaatca aacaaccaaa aaacagtaca aacaacaaag
    10501 gccgcaacgc aggaaggcag cagaagcgga aataaaaata tatgacaaaa aactgtagac
    10561 aaataaaaaa atttctttgt tctacagttt ttttgttaca attaaagaaa acgttcgcat
    10621 ttcgaggagg aatgtataat gatgtccaaa taatcaatgt atagtcgaat ggaagaagac
    10681 aaaatttcgc tagatgcact tgttcctaaa aatcatttag tccgtaaaat tgacaaatta
    10741 ttgtcatttg attttattta tctcattgtt gaatcaactt actcgaccat tggtcgccct
    10801 agcgttgatc ctgtcgtctt agtaaaatta gtattcattc aatatttatt tggaatttgt
    10861 tcgatgcgtc agaccattaa ataagttgaa acaaacctag cttatcgttg gtttcttggg
    10921 cttagtttaa ttgataaagt tcctcatttt tcaacttttg gtaaaaacta tgcacggcgt
    10981 ttcagagaaa ccgatttgtt tgaacagata tttatcatta ttctcgaata agcagttgat
    11041 gctagattta ttcatgaaga aattttgtat atggattcaa ctcatattaa agcgaatgcg
    11101 aacaaacgta tttttacaag agatatggca cataaagaag ccattttata ccaaaaacaa
    11161 ttagaagaag aaatcaatga ggcaagaatt gccgaaggca aacgtccttt tggttcaatc
    11221 attcgtcagg aggtggtcga aagaaaattt agcatcgcag atccagaaag tggttattat
    11281 gtaaaaacag aacgagaaaa acagtttgct tattctgctc ataccgttgg tgatgaaaat
    11341 ggttttgtat tagatgtcat gattacgcct ggaaatctac atgatagccg aatgctcgtg
    11401 ccacaaatcg aacgcgttaa atcacgttat ggtgtagctg ctaatgctgc gtacaaaaca
    11461 ccttggaacg tcaagtattt aatcgaacga aaactacgac ccattctccc ttatatcagt
    11521 ttcgcacaac tactcgagat ggctataaaa aatatgtatc taaatctttt atttgtgaaa
    11581 gttgtccatt attgaaaaat tgtacagaaa gccaaaaaca ccaaaaagaa atacatcgac
    11641 atatttggga agactactta gatgaagcag aacatttacg ccatacggtg tacaatcgtg
    11701 aaaaatataa aaaaagaaaa gaaacgattg aacgcatttt tgcagacgca aaggaaaaac
    11761 atggaatgcg ctacaccaag tatcgaggct tggaaaaaat caggtgcata caatgcttgt
    11821 ttttgtcgca atgaatctga aaaaattagc tacttggcag tgggagcata tgtcgttata
    11881 tcttttatta tacaaaaata gacaaaagta tcagtctaca ctcttagtaa agaggaaact
    11941 gatgcttttg tctacagtct tagagagtgg gacaatagtt ttttcttctt tacgtctttt
    12001 cctctccatg tattttccta attttcttaa tttaacgcca tgagtgccaa gccaatgtca
    12061 atcttggctc cttgtttcct tcataagtgg aaatccgttt ccgatagatc gaaccttctt
    12121 tttcctctaa aagttttttt acattctgct ttgaaataac gccatgtatt attcactaaa
    12181 atttgtcggt ttcgctcact ttttgctttc gtacattgac tacgaacagg tcagcaccga
    12241 ccactttcac attcgtaaat tttgaagaca cgtataaatc cacttttatc ttttcttcga
    12301 ctaaagcgtt gaccgcaaaa gcgacatctg tttcagaaat atattgttcc atatctaatg
    12361 gtaaaatttt ttggttgatg gtataatttt taaacataaa gcactcgctt cctttctgat
    12421 tgggtttggt cactttaatc ctacagggat gagtgctttt tgtgtacctt aaaatcagta
    12481 aatatccatc cgtgaaaacg ggtggctttt taacagccct agaagggctc accactcaca
    12541 gccctatctc tctttaacta tttaaatgga tctacatact cttgtttact tatactgtct
    12601 ctaattcgat catctgcttc ttgttctcaa atatatattt caattgtttt ttgatttaac
    12661 ccaaccgtac tcacgtagta tcctttggtc caaaagcttt tattcccata cttatatttc
    12721 aaatttgcat gtttttcatg gattaaggtt gcacttcgtc cttttaagta gcccataaaa
    12781 ctagaaatac taatctttgg cggtattctt actaacatat gaatgtgatc aggcattgca
    12841 tgagcttcaa tgatttcaac atctttcctc tcacacaatt ttcttaatat ctggccaata
    12901 cttactctta actttccata aatcactttt cgtcgatact tcggggtaaa gactatgtga
    12961 tacttacaat cccatctcgt atgtgctaaa cttttatcgc taaagctatt tcgtccacgc
    13021 gtagaaacgc gcgttttttt gtttcgtacg ctacgccaac gaaactgact aaagtcaata
    13081 aaaaaagtgg ggcaataatc agattttttt ctgactattg tcccactctc tttttattac
    13141 tcttcatctt ctactattcc actaattaat tagttgacag atacaatgct ttcacatcct
    13201 gcacgaacaa actcgggctc tgaaccaatc acttgcgagt tgataaattc aatgaccata
    13261 ggaagattaa tccgacataa agtttaatct gttgtccttc cattatcagt ttagaaatga
    13321 tattagtagg tgtaccgcct aaaagatcgc atagtaccac aaaatcattt aacgaatcta
    13381 tgacagtaag agcttttgtc tgtatgtttc agtgccatct tctggtaata aaggtaacgt
    13441 gtagatattc tcttgaggtc ccataatcat ttttgtactt tttttcaatt cctcacaaaa
    13501 ttttccgtga ctaatcaata tcaattttct actcattttt ggctctccct tatttcgaaa
    13561 tcacacacaa tgcagataaa gccacacaca agtaatacga taaaaatcgc tcgagttggg
    13621 tcatattttt ctttcctaac aaccaataaa cacctcgacg atcccagcga gtaagatttt
    13681 agataatatc atatcgatat agttttgcat attcaatgtc acatcgccaa tcgttggtac
    13741 cataaaaatt taatatcaat cattgttgca actaatgctc ctaccatgaa cactcttaat
    13801 aaagtcgcta cgtccgttaa tacagttaag cgagattaca ctagatgatc tgttcctgcc
    13861 aaggcaagag ccagaagcgg ttgcctgccc atttgccttt ggtgtgtcgg agctgtccga
    13921 taaagttcac cgcccgatcc gctctggctt tgtcgtagtg acattccgaa agcatgaatc
    13981 ggcttggtgt gtattccttt aatttgggat agccctttgg tctggtttct gccatcagct
    14041 accacctccc aacagagcct ccatcgcatc ttctgctccg acaccaccgc catcaccggc
    14101 aatgatacgg cttctggacg aaggcgtcag accgaactgc tcggaaaatc ggttcataat
    14161 tttcagatag gtctgggcaa taaacacctg tggtacctgc tgccaatagc cggatagggt
    14221 tttcacgatg ataccgtgct gggtaatgaa ttcctcggct tccttccagc gggcgtaggc
    14281 ttggcactaa ccggcaaagg cagccatatc cacatctgtc aagattccga tggcctccat
    14341 ctgctttgcc agacgcttcc attccttctt tgcctctggt tccagccact tcggacaggt
    14401 cggtgccttc ttttccggcg ttgactcttt tgcattgagt gggcgattgc ccggatttcc
    14461 ctccagcttc ttcatggctg tcggggttga ttttcttcct ctggttgcca cagtcgtcac
    14521 ctcccttcaa tgtatttctg ccagtagaca gagaacagag ccaagtcgaa acctgactct
    14581 cttctctatc tgctaacttg tatttcagtc cactcttctg atgaatcgca cccgcaggat
    14641 tttcaagatg ccgcaggcaa attccaagaa gtttctgacc gcatcataac catcgtagcc
    14701 gtgtagccac atccttccac ctcgccgccg tcctcgtcgc tgatataaat gattctgtat
    14761 tcgctcatct gttgttttct ccagtctttt gtttttttgt agtacatata tcactctaaa
    14821 agtgattatt atcaagtcgt ttgaaagcca taatgtacac aatcattcga ctggtttttg
    14881 gttcataatt gaccgccaaa gtgagcaaaa gaaagggacc tctgtctgtt tcagaagtcc
    14941 ctttcggtat ttggttctta gtggttcagg ctgtattctc aaacctcata ctcatcttcg
    15001 aggtctgcct ctgcaatgat ctggccgatg ctgtcgtaac gctggacctg catctggatg
    15061 gcatccttgt ctgctccctc gtccatcatc ttcagcagct tgtctgattc ctttttgtag
    15121 gcccagaaaa ggatctcctg aaggctggtc tcctgctttt tcttgcgaag ggcgaccttc
    15181 ttctcttcca ccgcctcgtt caacttggtg aactctgccc aagcaaggat accgtctcgc
    15241 ttgattgcga tctccacatc cttcagctcc gggatggtct ttgcaccctt gatctccttc
    15301 aaatatttct ttgccttttc gctcattgcc attagcgttt cctccttgtt tttgggattt
    15361 tcccctttgg ttgtgtgtat attgccatag tctctcgact atatcaagtc aatccacagg
    15421 catatactac acaatcattt ggagctgtat ttgtatacat tatgccgatt tatgagagaa
    15481 gaaaaggctc cagccgttcg actggaacct tgggggggtc ggtttcttag gcggcgtggc
    15541 ggtgtagacc ttgaggtagc tgatgttctt ccagacgtcc gtgcccttca gtctgaccat
    15601 gccgtccagc tctcggtcga gggtgaactt gcggaaaagc ttgatgcggt tgaggctgtt
    15661 tgctagctcc ttgccgttct tggcatccag aactgcgtcc tcgatctccg caatactaat
    15721 ttcctgtcta aaccaaaggt tgctcaaggc ttcgggaatg ccgtttgcgg tctgcagggg
    15781 gttgaaagtt tccaatacag caggggtcga aaccacctgt ccgagctgga attttgcgtt
    15841 ccctaaaatc atctgcataa tttcttccat gtgaatcatc catttctgcc gttgtggtat
    15901 tccctttggc agtacagtac tatatatcac tctgaatcgt acatttatca agaagcggat
    15961 ttttgtggaa tcaccaattc gatataatag taattgcatg aaaaatctaa gcactggatt
    16021 ggtaggtgat gattatacgt gacgatcaca tttacacctg tgattcctgc cactacagct
    16081 tccctgccga cgaacagcca gagcgttgtc ctgactgcga aaagacagca actcggctgg
    16141 ataccgaaat cgaaactgaa gattactatc gtgttcgtgc tgaaatcaaa gctgagatta
    16201 aagctttgaa tgcgggttaa cgacaggtac tgaccggcgg ctcaacaccg ccggcttttt
    16261 attaccacat cttgcggaat tcgctgggac gaacaccttc caccttgaaa aaagttctgg
    16321 aaaaatagtt tgcatctgtt atgccaattt ttgttgcaat ctcttccata cttagatttg
    16381 taaagcgtag caagtgcttg gcctgtgtga ttcgtttctg attcagataa ccgacaacag
    16441 ttgtaccgta agtttcacga aaaatcttag ctaagtactg tttattgata aaaaactctg
    16501 cactcaactg atccagcgta atcttctccg tgtaatggtc atccagatat tgttttatgt
    16561 tctgcaattc catttttttt gtagagaaat tcggcacttt gtcaggattc caagaatctt
    16621 ccatcaaatg catcagcagt tcactgagta gaacattgat tttcatatcc cggacatact
    16681 cctgtccctc tgccaccata tgaaggctcc tcagcaagtc agcatactta tctgtttttg
    16741 atgttttaaa gaccggttga ccacctcgtt caaggtattt tttgtagact tccgtcatac
    16801 caatgccatt aaaatggcac caccgcaaag tccacaaatc atcttctgat gactcatgga
    16861 catacggctt caaacagtca ataaacacca tatctcctac ttgaagcgta tatttctgcc
    16921 ccttatactc caatgtgcct gaaccaactt ctaccaggaa acaaaggtaa gatgacaatc
    16981 ctgtacgcct agatgtatgt gccttcagag cagtcagctg tccaatttcc tgcaaataca
    17041 aaagagagga tcttgcaaag aacgatgccg tgtacagcac acgatttgac ttcaccgatt
    17101 tcaaagcgtt agaaaacagt tcctccatta caagatttct cctttctttt cttactcgaa
    17161 catttccgcc agttcagcag ccataatagc ttccatagct tcggcggctt ccttgttctc
    17221 tgcgctgacg ctgatatagg tcttcagctt cggctcagta ccagaaggac gaaccacaac
    17281 agagcagtta tcttccagca taaacttcaa aacatcagac ttcggcagac cattcaggcc
    17341 cttactgtag tctaaagttt caataacctt cttaccacca attatatcga gacccttgtg
    17401 gaattcaacc ataatttcct gcatcttggt aaaacctgcc gcaccgtcaa actcgaagga
    17461 atgcagggtg ttcaaacaat agccatactt tgtataaatc tcgttcagct tatccagcag
    17521 gctgataccc tgagtcttgt agtaagagaa catttcacag attagataag cagcgttaac
    17581 accgtccttg tcacggacat agctaccgga cagatagccg taagattcct caaagccgaa
    17641 gatatagctg tcaaccttac cagccttttc caacatacca atctgctcac cgataaactt
    17701 aaagccggtc agcacatcga tggtacgaac accatagttg gatgcgatac gttcacccaa
    17761 atccatggtg acgatggtct tgaccatcac agggtcagca ggcatttttc catgcttaat
    17821 tctctgagaa cagatgtagt ccagaagtag cagaccagtc tcattaccag acaacagaac
    17881 atagtcacca tgtgcgctct taactgcgat accaacacgg tcgcagtcag ggtcagtggc
    17941 caacagcaga tccgcattgt acttcttggc atactccata cccagagaca tagcttcctt
    18001 aatttcaggg ttcggataag gacaagttgg gaaattaccg tccggcagac gctgttcttc
    18061 cacaacggtc acattgctgt atcccatctc tgccagggtg cgggtaacag gcaccagacc
    18121 ggtaccattc agaggagaat acacgatagc aacattcttg tccacttctt cgccaaacag
    18181 gacggactgc cccttcactt cttcgatgaa agcagtcaga acttccgact gaatggatgc
    18241 aatcttagaa gcatcgccat ccatctttac atcagcaaag atgtccagct tttcgatttc
    18301 tgccagaatt gcagtagcag cctcggtagt aatctgacag ccgtctgcac cgtatacctt
    18361 gtagccgtta tatttagaag ggttatgaga tgccgtaatc ataacaccgg catctgcctt
    18421 cagataacga gtcgcaaaag atactgtagg aacaggaagc agttctggcc acaagtgcac
    18481 ctgaatacca tttgcagcaa agatgtctgc ggcagtcttt gcaaacacat cagatttgat
    18541 acgggaatca tagccaacca ccacggaagc ggcttcatag ttcttattca gatagtctgc
    18601 cagaccttgg cttgccttac ctacggtgtg tacgttcatg cgattagtgc ctgcaccgat
    18661 ggtaccacgc agaccaccag taccgaaaga caaattttta tagaaagagt cctcaatgtc
    18721 cttctcagaa taggaacgca gttcttctat aatatctgca ttggcatgat tcagccaacg
    18781 ctcatattct actttataat tcatattatt atcctctcac atccagaaac tgaatcgacc
    18841 cgtgaatgct gatttcagta gaatcggccg gcacaaaaag gcagtccccc ttatagatgt
    18901 ccaacgattc attatcaaat cgaacggtac cacagccatt gacacacagc aacacacgga
    18961 agctgattga atccgcacga tagtgtaccg tctgtcttct ctcagtgttg acaatcatac
    19021 gagagacctc gaaatgcttg catctgcaaa gcaattccgc agctacacca gcacgatatt
    19081 ttagaacacg aagcggctgt ttcggttctg acaacccttt caagttcgct acttccagcg
    19141 ctttgtctac atgaagttct ctctttttac catttttgtc aacacggtca taatcgtaga
    19201 gacgataagt cagattggaa ttttcctgaa tttctgcaat tagtgcaccc gcaccaatcg
    19261 catgaatcgt tcccgcctca ataaagaaca catcattctt tttgaccgga accttctgaa
    19321 gataacgctc aacagttccc tgagcaatcg cttcacggat cacagcttct tctgttattt
    19381 tctgcagccc atagattaag ctggcatcca gtacatacca catctctgtc tttccaagct
    19441 gcccgttttc tttttcaatt gcatattcat ctgtgggatg tacctgaaca gacaaatcct
    19501 ttttcgcatc aatgaatttg atcaggatcg gcaattctcc tttggtctgc ggatgcgttc
    19561 ccagaaactc tgggtgttct tgcaacacct ccgtcaaggt catcccctga tactctgacc
    19621 agacacaaca aagctcggtc catccgggtg cgtagagcat tccaagtttc cgcaagagga
    19681 gacatctcaa ttccttttgc aaattcatca ttcaggcggt tcccccccac agataatcct
    19741 ttgcggaagg cttcaataaa aacggtttct gtataacgag ccctcctccg ttttttaata
    19801 ctctaactca aatttctgtt tatctttaac attgatatcc ttgccaagct gtacttcaat
    19861 cagcttcagc tcagtatctg ccataatggt atgccgacag ccagcactca tggtgatcac
    19921 atcgccagcc ttaacaacct gctcatatcc atccacgatg gtcttaccag taccggccag
    19981 aaccacccac acctcgtcac ggttcttgtg gctgtgataa ttcatggagt gactagcatt
    20041 cagagtaacc ttaatggtca tgctctctgc ttctacatcc ataaccttat aactacccca
    20101 agacttctct gcgaacatga tctgctgctc gaacttgtca acgaaaggct taatatagct
    20161 ggactgttcc ttatcggaaa ccagaatacc ttcccgagat gcacagatga ccacatcatg
    20221 cagacccata gccaacacag gaacatccag ttcgttcagg atgtgtacgc catcgcaatt
    20281 ctcattcata acacctttac cgataatgga ttcctccata ccttcggtca gagtattcca
    20341 agtccccaga tccttccaca taccagcaaa acgctgtacc tgaatcttgt cctcatgctc
    20401 aacaacggca tagtcgaagg aaatcttagt cagagtatcg tatttgttga acagatcctg
    20461 ataatctaca aagtcaatca gctgatgtgc acgctccatg acatacttca gcttgtagac
    20521 aaacacacca ccgttccaca gtgcaccctg tgcgatatat tcctttgcca cagcctcaga
    20581 aggtttttcc ttgaaggtat ttacgaaagc agtctgctct gcgctctcag gaatgatgta
    20641 gccgtacttc tcggaaggat aagtcggttc aatacccatg agcaccagat tagcttcgcc
    20701 tttgtcagcc tgatcggaca gagatttcag ggcctcaaaa tagtcatctt ctacgtaagg
    20761 gtcaaccggg caaacaacta cggactcttc tggatcaatg cccatcacat ctaccagata
    20821 agcggtagcc aatgcgattg ccgggaaggt atcgcggcga caaggctcta cagatacacc
    20881 cacatcctca ccaagttggt tatggatagc agaaacctgt gtcttagagg ttgcaatcgt
    20941 aacagttgcg tcgttatcta ccttcttaat ctggcggtag acacgctgaa ccatggattc
    21001 atatccatct tcagttttga aaatcttaat gaactgcttg gaacggatgt cattagacag
    21061 cggccaaagg cgcttaccag aaccgccaga cagtaaaaca atattcataa tgatctctcc
    21121 tttttataag aaagaggctg ctgcaatcct caaaaatcac agcaacctcg gtaaattagc
    21181 gttcagcacg catcgggtta ttcaggaagt cctcgtagga cagacggatg ccatcttcca
    21241 actcagtctt atatgtccat cccagcttgg ttgccttgga aacatccagc agcttacgag
    21301 gtgtaccgtt cggcttgctg gtatcccaca gaatctcgcc ttcgtaacca ataacctttg
    21361 ctaccatctc ggtcagttcc ttgatagaca gttccttgcc agtacctgca ttaacagtct
    21421 cgtcaccgct atagttattc atcaggaaca cacacaggtt tgccagatcg tcaacatata
    21481 ggaattcgcg cagaggagaa ccatcacccc agcaggtaac agtaggcaga cctgcttcct
    21541 tagcctcatg gaagcgacga atcaaagcgg ggagcacatg actgtgagta ggatgatagt
    21601 tgtcgttagg tccatacagg ttagtaggca taacagagat atagtcagta ccgtactgct
    21661 tgtttagaaa ctcacagtac ttcaggccag aaatctttgc cagtgcataa gcctcgttgg
    21721 tcttttccag ctcacttgtc agcagacagc tttccggcat cggctgcggt gccatacgcg
    21781 gatagataca ggaagaaccg aggaactgta acttcttaca gccattctcc catgcggagt
    21841 tgatgacgtt catttccaga atcatgtttt catacatgaa gtctgccaga gcgttctggt
    21901 ttgcgataat accgccaacc tttgcagcag cgagaaatac ataatccggc ttttcctgtg
    21961 cgaaaaatgc ttcaacagcg tcctgacggc acaggtcaag ctccttatgg gtgcgggtga
    22021 taatattgtt gtagtcctga cggttcagct cgcgcacgat tgcggaacca gccatgccac
    22081 gatgaccggc tacgtagatt tttgcgtctt tttccatcat aataatgctt gctcctttct
    22141 aaatttagag catctctcat tgcatgagag aaatcaattt gtctgcgatt tcgctcgcag
    22201 catcataaaa tgtatcaaaa tttgaggtgt ctagctgttt gaggtcctca atcacccgct
    22261 ctacgcggtg gaggttttcc ggtgtgacgg aaattgccag acccagtttt tgcaatttac
    22321 aaatagttgt tctatcttcc aaaactgtat cgcgggcgaa cagtgccatc ggacgctgtg
    22381 cgagaataca ctccgcaaca gttccccatc cagcctttgt aataacgtaa tctgccgccg
    22441 ctatatagtc ctgtgtattg ggggtgctgt tagaaataac ggttacgttt tctcccacaa
    22501 gcggaacacc tagcgttgta atgaaatcat acggcgcgtg actgacatca atcggctgtt
    22561 tgaaccgcgc ggacataccg agtgcgacaa atacaggcgg tttttcatgc tgtgatttaa
    22621 tgcgttgcac ttcgtcgtta tgaatcggac gcgccacaac ggaagtcggt gttttttcgc
    22681 tgttttggag aaattccaac atttcttcgt tatttagcgc ataaagcatg gtgtgctgaa
    22741 ttttaccgta acgcttggca tattctttcc aaatatgttc gggtaaaaat tcgcgataca
    22801 gctctgtcca agtgaagttt cctacataca ggaaaggtac tcctgccata tcgcaggcat
    22861 ccattgccca gattggcata tcgcaaagca ctgcgctgac agcttctcgc ttgagccatt
    22921 cggcttctgt ctttgccaaa gaaggaacct ttgccaaaaa atattcacag atctcggtca
    22981 gcttacccgt gtccaccagt agggtgccct cacggttaat cagaccgata tctgtgttta
    23041 ttggacgata gcgcacgcgg tctatcagct ctttgggcag ttcctgctcc aaattgcgct
    23101 ttgcaaaatc cagatgattt gcgccgcaaa ccagtgtaat cttcgcttcg catcggcaga
    23161 gtgttgccat aataatgggc agattccgcg cgatatgtcc gaagccgtga ctggaaagat
    23221 agaaacagat atgtttattc aatgtcagct tacttgactg cgttcttcat tgcagcttcg
    23281 cgctttgcca attcacggtc atgctttgcc atgatgttaa ccagttcctc gtaagaggtc
    23341 ttctgcggat tccaaccgag aacggtcttt gccttagtcg ggtcgcccca caggttgtca
    23401 acgtcagtcg gacggaacca ctgtggattt acacaaacga gcatcttgcc ggtttcaaca
    23461 tcgtagccct tttcatcaat gccagtgcct tcccaacgaa tggtcatgcc atttgctgcg
    23521 aatgccttct cggtgaagtc acgaacagtg tgctgctcgc cggtagcgat cacgaagtcc
    23581 tcgggagtct catgctggag aatcatccac atgcactcaa catagtcctt tgcatagccc
    23641 cagtcacgca gggagtccat gttgcccagt tccagatggt cctgcaggcc ttctgcaatg
    23701 cggccggcag cgagggtgat cttacgggtg acgaaattct caccgcgacg ttcggattca
    23761 tggttgaaca gaataccgtt gacagcgaac atgccataag cttcacgata ttccttagtg
    23821 atccagaagc catactgctt tgcaacagca taagggctgt aaggatgaaa cggagtggtt
    23881 tcacgctgag gaacttcttc aaccttgcca tacagctcag aagtagatgc ctgataaatc
    23941 ttagtcttct tcgtcaggcc cagaatacgg caagcttcca gaatacgcat aacgcccaaa
    24001 gcatcaacat cgccggagta ctcaggaaca tcaaaagaaa cctgaacatg gctctgtgca
    24061 gcgaggttat agatttcatc aggctgaaca atgctgatga tacgaatcag agaagatgag
    24121 tcggacaggt caccgtcatg aagggtgatc ttgcccatca aatggtcaat gcgagcggta
    24181 ttggaaatag aagcacggcg agtgataccg tgaacttcgt agcccttctc tagcaggaaa
    24241 tctgccagat aggaaccgtc ctgaccagta ataccagtaa tcagcgcttt tttcatgata
    24301 cattatcctt tcctatgtaa gtgataaggc catataaatg gctcgtaatt tctaatactt
    24361 ttcaagaata cacgataatt ttcgatattt catagaacaa tctacgcacg cattagcaca
    24421 atattagttt ttcgtgaatt ggaatggcaa cagttttttt gacaaatttt ataaggtgca
    24481 gaacttcttt ccgtatgcta ttccgattgt ccttgacaat gagcctcctc ggatgtgacg
    24541 atcttcggca ggagctaaca gttaaagtta gactgcctcg ctagcgttag cacatccgag
    24601 ctcattatca aggattcgct gcgccaatct ctggttacgg taaccaaccg gtgtctcgta
    24661 gccaagtgta ccgtgcaacc gaaggcggtt ccaccaattg acatagtcaa ataactccaa
    24721 atccaattgt tgtaaggttt caaatgtgta ttgatagaca aattctactt tcaacgactt
    24781 ataagttgat tcagctacgg cattatcaaa aggacagcct ttatgactca atgatcgatt
    24841 gatgtcaaaa gttgttgata attcatcaat agcttggtta tcaaactctt ttccacgatc
    24901 agtatgaaaa atctcaacct ctgtcagagg ttg
//
LOCUS       DAYYGS010000039        24656 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000039 DAYYGS010000000
VERSION     DAYYGS010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.039"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            180..1919
                     /gene="yfcC"
                     /locus_tag="ACHA44_001467"
     CDS             180..1919
                     /gene="yfcC"
                     /locus_tag="ACHA44_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFM8904750.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2076..3260
                     /gene="iadA"
                     /locus_tag="ACHA44_001468"
     CDS             2076..3260
                     /gene="iadA"
                     /locus_tag="ACHA44_001468"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFM8904751.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3371..3574
                     /locus_tag="ACHA44_001469"
     CDS             3371..3574
                     /locus_tag="ACHA44_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8904752.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3675..4337)
                     /locus_tag="ACHA44_001470"
     CDS             complement(3675..4337)
                     /locus_tag="ACHA44_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904753.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4350..5852)
                     /locus_tag="ACHA44_001471"
     CDS             complement(4350..5852)
                     /locus_tag="ACHA44_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904754.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6400..8079
                     /locus_tag="ACHA44_001472"
     CDS             6400..8079
                     /locus_tag="ACHA44_001472"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8904755.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8206..8685
                     /locus_tag="ACHA44_001473"
     CDS             8206..8685
                     /locus_tag="ACHA44_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HFM8904756.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8904..9584
                     /locus_tag="ACHA44_001474"
     CDS             8904..9584
                     /locus_tag="ACHA44_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFM8904757.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9592..10923
                     /locus_tag="ACHA44_001475"
     CDS             9592..10923
                     /locus_tag="ACHA44_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HFM8904758.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10938..11900
                     /locus_tag="ACHA44_001476"
     CDS             10938..11900
                     /locus_tag="ACHA44_001476"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HFM8904759.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            12011..12349
                     /locus_tag="ACHA44_001477"
     CDS             12011..12349
                     /locus_tag="ACHA44_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFM8904760.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12428..13135
                     /locus_tag="ACHA44_001478"
     CDS             12428..13135
                     /locus_tag="ACHA44_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HFM8904761.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(13238..14719)
                     /locus_tag="ACHA44_001479"
     CDS             complement(13238..14719)
                     /locus_tag="ACHA44_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904762.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            15028..15564
                     /locus_tag="ACHA44_001480"
     CDS             15028..15564
                     /locus_tag="ACHA44_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904763.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            15642..17012
                     /locus_tag="ACHA44_001481"
     CDS             15642..17012
                     /locus_tag="ACHA44_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HFM8904764.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            17079..17414
                     /locus_tag="ACHA44_001482"
     CDS             17079..17414
                     /locus_tag="ACHA44_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904765.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(17495..18352)
                     /locus_tag="ACHA44_001483"
     CDS             complement(17495..18352)
                     /locus_tag="ACHA44_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904766.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            18459..18974
                     /gene="tadA"
                     /locus_tag="ACHA44_001484"
     CDS             18459..18974
                     /gene="tadA"
                     /locus_tag="ACHA44_001484"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFM8904767.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            18997..19081
                     /gene="ffs"
                     /locus_tag="ACHA44_001485"
     ncRNA           18997..19081
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHA44_001485"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            19291..20676
                     /locus_tag="ACHA44_001486"
     CDS             19291..20676
                     /locus_tag="ACHA44_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HFM8904768.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            20713..21390
                     /locus_tag="ACHA44_001487"
     CDS             20713..21390
                     /locus_tag="ACHA44_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HFM8904769.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            21398..22162
                     /locus_tag="ACHA44_001488"
     CDS             21398..22162
                     /locus_tag="ACHA44_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HFM8904770.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            22164..22823
                     /locus_tag="ACHA44_001489"
     CDS             22164..22823
                     /locus_tag="ACHA44_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HFM8904771.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(22853..23362)
                     /locus_tag="ACHA44_001490"
     CDS             complement(22853..23362)
                     /locus_tag="ACHA44_001490"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFM8904772.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(23474..24472)
                     /locus_tag="ACHA44_001491"
     CDS             complement(23474..24472)
                     /locus_tag="ACHA44_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8904773.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT         7989 a         4178 c         5193 g         7296 t
ORIGIN      
        1 tacttaatat aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata
       61 gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc
      121 atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt
      181 tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc
      241 atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc
      301 cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta
      361 ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg
      421 tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc
      481 cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac
      541 tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg
      601 atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct
      661 ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg
      721 tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg
      781 gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag
      841 ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag
      901 aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga
      961 tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga
     1021 gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga
     1081 cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat
     1141 atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt
     1201 tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat
     1261 tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag
     1321 gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca
     1381 ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat
     1441 ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc
     1501 ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga
     1561 tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc
     1621 aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa
     1681 tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc
     1741 agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc
     1801 taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat
     1861 tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt
     1921 actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc
     1981 attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct
     2041 atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag
     2101 tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg
     2161 ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg
     2221 agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag
     2281 gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg
     2341 gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt
     2401 tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt
     2461 cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg
     2521 ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt
     2581 ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag
     2641 ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa
     2701 tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac
     2761 cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg
     2821 ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac
     2881 tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac
     2941 aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg
     3001 ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa
     3061 tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa
     3121 aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg
     3181 atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga
     3241 ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct
     3301 gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg
     3361 aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg
     3421 tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa
     3481 agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa
     3541 agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa
     3601 gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat
     3661 cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact
     3721 tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt
     3781 tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt
     3841 ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg
     3901 actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca
     3961 attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta
     4021 gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa
     4081 gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt
     4141 gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa
     4201 agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg
     4261 aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat
     4321 tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat
     4381 cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg
     4441 gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa
     4501 acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt
     4561 ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc
     4621 tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca
     4681 actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta
     4741 cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta
     4801 atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt
     4861 ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt
     4921 gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc
     4981 attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc
     5041 ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag
     5101 ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc
     5161 atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc
     5221 aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg
     5281 tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt
     5341 gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc
     5401 attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca
     5461 gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa
     5521 taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta
     5581 agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt
     5641 tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct
     5701 ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca
     5761 aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt
     5821 gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt
     5881 tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa
     5941 catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga
     6001 aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta
     6061 gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt
     6121 tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt attctggcta
     6181 tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc
     6241 aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat
     6301 agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga
     6361 catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc
     6421 ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat
     6481 atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc
     6541 gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta
     6601 acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta
     6661 ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa
     6721 gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt
     6781 gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat
     6841 ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct
     6901 atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta
     6961 gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa
     7021 atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt
     7081 gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga
     7141 aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa
     7201 aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat
     7261 ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa
     7321 aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc
     7381 actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat
     7441 cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat
     7501 cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa
     7561 ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac
     7621 aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt
     7681 gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc
     7741 ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt
     7801 gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga
     7861 ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca
     7921 atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc
     7981 cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca
     8041 gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc
     8101 tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag
     8161 catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca
     8221 aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat
     8281 gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata
     8341 tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg
     8401 aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt
     8461 cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt
     8521 ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa
     8581 gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat
     8641 ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa
     8701 gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct
     8761 gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt
     8821 agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac
     8881 agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa
     8941 ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga
     9001 cagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg
     9061 accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg
     9121 ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg
     9181 gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct
     9241 atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag
     9301 aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg
     9361 cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc
     9421 cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt
     9481 acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata
     9541 gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca
     9601 aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta
     9661 agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat
     9721 gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc
     9781 aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg
     9841 gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg
     9901 atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac
     9961 cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga
    10021 ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc
    10081 acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg
    10141 atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat
    10201 ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg
    10261 cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt
    10321 ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta
    10381 aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc
    10441 atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg
    10501 aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata
    10561 tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag
    10621 ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta
    10681 cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat
    10741 atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta
    10801 gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg
    10861 tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct
    10921 tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg
    10981 ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag
    11041 gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa
    11101 cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag
    11161 gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc
    11221 agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc
    11281 ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga
    11341 tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg
    11401 tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt
    11461 cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc
    11521 cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg
    11581 gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag
    11641 agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt
    11701 ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca
    11761 atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa
    11821 aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata
    11881 agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca
    11941 aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag
    12001 gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga
    12061 tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac
    12121 tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga
    12181 tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg
    12241 aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga
    12301 aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa
    12361 ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga
    12421 aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat
    12481 tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata
    12541 cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca
    12601 aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta
    12661 caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc
    12721 tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt
    12781 cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt
    12841 ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat
    12901 tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc
    12961 gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc
    13021 gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac
    13081 attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa
    13141 agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac
    13201 catttatttg gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct
    13261 cataaccttt tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg
    13321 tattgtgcgt tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat
    13381 ctgtaatgat caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa
    13441 ataactgctg caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca
    13501 aatggatgag taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa
    13561 ggtattgact gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc
    13621 ttaatttctt ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa
    13681 gtgtagaaaa actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa
    13741 gaaaataaaa ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta
    13801 agtaagtcat caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa
    13861 aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaaat
    13921 aattaaagta ggctctctct gtttctgtct cttcacaagg gcgactcact gaaaaagaag
    13981 taaagtattc tgaataatca tgtgtatgct gcaataagtc catcaattca gtgatttcag
    14041 aagaagtttt tggaaaatat gcctgttgtt tgtttcgtaa tatgaataaa gttaacagca
    14101 agtagatatt tttttcttgt tttgaaattt tttctatttg ctcatctgta ctgttcattt
    14161 ttagtgcatt taattcaagt gaggaaaatg gccaatcgtt acctccaaat gtatctgaaa
    14221 gaaaaaagta aagatagaga cggatagata actcatttcc ttcaagagta cagcaatttt
    14281 ttacagatag gatccgtaaa tggaattttt ttaaggaatg attgagctgt tttgtcagtt
    14341 tttcgagata ggagggcgat atataaagtt gttcaagtaa atattttttt ggtacagcag
    14401 tttgtggata attgattagt aacatcaaat agttaaaacg attggattct tttaggtagc
    14461 ataattttaa cttagaaaaa acagctaact ttttagtttg atcggtttct ttggtttgga
    14521 tctctccttt atgattagaa taaataaatt catcaatcaa taaaaattgc ttgagtgata
    14581 catttatttt tcggatcagt tctttgattg tccgttttgg aatatgtaag tcattggata
    14641 attcttgtaa agaacaatta tttttttgaa tgaccatttg tagaacaagt aactcttttt
    14701 ttcttgcttc aattagcata aatacctcct ttttattctg tttttgtata tagttagaca
    14761 aaaactgtga gtggaaagtc atttcagtct gtgttattct cacagagtgt cgccttcttt
    14821 tttatataac gttatttaat ttttttgtta ccttgtttat tctattgttt atttcctagg
    14881 gtattttgtc aagatattct tgtttttaag gcggaaaaat tgatttttaa tggatataac
    14941 taaatatgtt ttgataatta tcaataatat ttttagaatt atttatgtta tttatttatg
    15001 ggtagatgaa aagagacgga ggtaagaatg atggggagag taacaaatta tagtaaagaa
    15061 tatttgctgt tcaaaagtat ggtatacgtg gaagaatatg gaatgaagtc aattactgca
    15121 agagagttag cagagttctg cggttgttct acatacccga tttatacaca tttcaaatca
    15181 atcagcgggt taaaagagaa gatattagaa gaaatcactg tttattttga aaactactta
    15241 gctgaatgca gttccgatga tgttttaagc attgtctact tattgaaaga ttttttttct
    15301 atccatgaaa gtattcgtga aatcattgca aaatctgatt tagacatgga ttcgctattt
    15361 aagcggccat ttttctcata tgtcaaaggt cacacgaaga tcaaagaaga aaagtcgctt
    15421 gaattggtat ggcttaatgc attgggaagt ttgcgctcag atgattcttc tcaagatttt
    15481 cttgaaatga tgaacgctgt ttgggataaa aacgaggtga cggatgaata cttggaaaat
    15541 atcttaaagg aacagtcacc ttaggcagca acatgaaaaa gtagatcagc cacctttatt
    15601 tttcgtaaaa aagattgtaa aatgtctaac gaaacattgg aatgaaggag gaagcgacaa
    15661 cgttaccaca gcaatttatc aaaaaatatc gtcttctttt aggcgaagaa gcatccgatt
    15721 ttttttctgc gcttgaacaa ggaagcgtga aaaaaggatt tcggtggaat ccactaaaac
    15781 cggcaggatt ggacatggtc caaacttacc atagcgaaga attacaaccc gctccctaca
    15841 gcaatgaggg atttcttggc actgtcaatg gaaagtcttt cctccatcaa gctgggtatg
    15901 aatatagcca agaaccaagt gcaatgatcg ttggaacagc tgctgcagca aaacccggtg
    15961 aaaaagtttt ggatttgtgt gcggctcccg gtggtaaatc aacccaatta gctgctcaaa
    16021 tgaaggggaa agggttatta gtgacgaatg agatctttcc gaaacgtgcg aagattttat
    16081 cagaaaatat agaacgatgg ggagtttcga acgcaatagt gacaaatcat gcacccgcag
    16141 aacttgtccc tcatttttcg ggcttttttg atcggatcgt ggttgatgct ccttgttcag
    16201 gagaaggaat gttccgaaaa gatccaaatg caataaaaga gtggacagaa gaatctccgc
    16261 tatactgtca aaaacgtcag caagagattt tgtcttcagc aataaaaatg ctaaaaaata
    16321 aaggacaact gatttattcg acttgtacgt ttgcaccaga agaaaatgaa gagatcatta
    16381 gctggcttgt agaaaattat ccagtaacga ttgaggagat tcctttgact caatccgtta
    16441 gttcaggacg ttctgaatgg ggaagcgtag ctggtttgga aaaaacaata cgtatatggc
    16501 ctcataaaga tcagggtgaa ggacattttg ttgcaaagct gacttttcat ggacaaaatc
    16561 agatgcataa agaaaagaag acacgaaaaa aaagtaaagt gcaaatgaca aaagagcagg
    16621 aaaaactctg gacggagttt tcaaatgatt ttcattatga agctactgga agacttttag
    16681 tgttcaacga ccatctttgg gaagttcctg agttagcccc ttctctcgat ggtttaaaag
    16741 tcgtacgtac aggtttgcat ttaggagatt tcaaaaagaa tcgatttgaa cctagctatg
    16801 cattagcgct tgctacaaaa aagatagaga atataccttg cttgccgatc actcaaaagg
    16861 aatggcaatc ttacacagca ggagaaacat ttcaacgtga tgggaatcaa ggatgggttt
    16921 tgctagtatt agataagata cctgtcggat ttgggaaaca agtaaaagga acagtaaaga
    16981 actttttccc taaaggattg agatttcact gagtggatta gttcgtttat ttttttgtct
    17041 atgctatgat ggagatagat aaaaaggagc tgatacttat gaaaacatca acaaaagtaa
    17101 caatcggatt aagcgtagca gccgctgcca gtgtagcgac agcagtcgtt gtatcgggaa
    17161 aagtaatgga aaagatccat catatgacta ctcggacgaa agtaaaaaaa ttcgtctatg
    17221 acaaatttga tggaaatgaa aaattactag aaatcgtaga tggtctttca gatagtgact
    17281 tggattcttt gatgggaatg ttagcaaaaa tcaaatcagg aaaaaagaaa atctctgttt
    17341 atggtgattc aatcaaagat tcaaccgaag atgtaaaaga ccggctaatg cattttgttg
    17401 aaaaaatgat gtaagagaaa ctagcttttg aacactattc attcgaaata aggggttgtg
    17461 acaaaattat tgtcacaacc cctttaatct atctttaatt atgttcatga agcaataaaa
    17521 gatagcaaaa cagtaaatcg ttggcttttt ttagattgaa accagactgt tcttgaaatt
    17581 tttcgattcg atattttaga gtattacgat gaagaaacag tgtttttgca gcagagctga
    17641 cattgcctaa ttcattccat aattcttgaa tgatcacttc catttcattt tgtttgatca
    17701 gttctatgta acttttgatc aacttactat cttttacagt atcttttgta tagtaatgta
    17761 aagccacatc ttgcatagaa aaagtacggc tgtgagtata aagattgttt tgctctgtta
    17821 aaaaaatttt tctttcttca gcaaacaaag ggacgaggtc ggtgccaggt gaatggaaag
    17881 aaccaacgaa tacagacgtt gtcgtatcaa aatcagcatc taaagataga aaaataccgt
    17941 tgatttcagt agcatcaaga taatcacggt ttatttgttc gactaagagc gcatctgtgt
    18001 ctgtataaaa aaagaaatca gcagaatagc gaaataattc caaaatagta tcttgccatt
    18061 ctttttttaa gaattctcct tttgattcga cgtgaagctg gatcaaacga aagctttttt
    18121 cttgaacaca agaagcatct ttaaaaagat agtgatacca tgggtgtttt ttttgatcgt
    18181 tgattacagg gaataacgct ttcaaaaggc tggtttcttg ttgagataat gaatcttgtt
    18241 tgatccagat aaaagcatca tcaattgcaa aagaaaggat atccgcttct gatgctggag
    18301 agtttttttt ctgtgcttgt gggtaaagag caagtaattt atccattttc atcttgttca
    18361 cctcttgtat ttattttacc atagaaaaaa aagttagtta cagccttttt atcatatgct
    18421 aaagtaaaac aagaaaaaac aaaggcaggg aagggtaatt ggtaaataca aatcttgatg
    18481 cagtagaaaa agaaaaatgg atgcgccttg cattggcaga agcaaagaaa gcagaaatgc
    18541 ttcatgaagt tccgattgga gcagtggttg tattggatgg aaaagtgatt ggaagaggat
    18601 ataaccttag agaaaccacg caagatgcta ctacacatgc agaaatgctt gcaatcaagg
    18661 aagcctgtga aaaagtggga agctggcgct tggaagatgc tgcacttttt gttaccttag
    18721 agccttgtcc tatgtgcagc ggcgcgatga ttttgtctcg tgtagcagaa gtttatttcg
    18781 gcgcgtatga tccaaaaggc ggaacagccg gtaccttgat gaatcttttg gaggacgagc
    18841 ggtttaacca ccaagcatat gtagaaggtg gcattttaga agacgaatgt gggatgattt
    18901 tgactgactt tttccgaaag ctccgccagc gaaaaaagaa attaaaaaaa gactggtcaa
    18961 cgaatgaaaa ataaggtata ctattctttg ccgaaaggct aggacaatgg tgggcttgtg
    19021 aagcgtgtca ggtctggaag gaagcagcac taagcaggtt ccgccatgtg tctgattata
    19081 atgaaacgac aacagacttt cgggtctgtt ttttttattt ttatagtact gtaaaaatga
    19141 atgttcctga ttttttagcc agactgattt atcgaatatt gtacttatac aatttgcttc
    19201 aaggcaggaa aaactctgaa atagagcttg tttcaatgga agaaaaagct gcctatggta
    19261 tgataaagat aaagagaggc gagagggtac atggaaaaaa tagtgatttt acatacgaat
    19321 gatctgcatt ctcatttaga aaattggcca aggatcagaa ggtttttgga tcaacgtaaa
    19381 cgagaaaacg aaaagaaaga aaaaacgaca acgattgcag tagatctagg tgattttgtg
    19441 gatcgttggc atccattgtc agaagcaacg aatggacagg aaaatatctt gctgatgaat
    19501 gaagttggtt atgatgcagc gacgatagga aacaatgaag gcgtaggaaa ttccaaagaa
    19561 gagttgaatc atttatacga tcatgctgag tttgatatca tcctggataa cttattcgat
    19621 aaaaatacgc tccagccacc ggcatggacg aaaccatata aaatcattac aacgaaacac
    19681 cagacgaaaa tcggactaat tgcatttact gctccatttc cgcttactta taatccgaat
    19741 ggctgggaca tacggaatcc ttatgatatt ttgccagaat tagtcgaaga tttacgttca
    19801 caagtggatg ttttagtttt gatgagtcat ctcgggattc aggatgattt ccaaattgca
    19861 aaagaaatac cagcaattga tgttatttta ggatcgcaca cccatcattt attcagagaa
    19921 ggtcacgtaa tcaatgatgt ccaaatagca gccgctggaa agtacgggca gtatgtcggt
    19981 gaagtccatt taaccatcga tgaagagaaa aaaatcctta agcattcagc caaagccatc
    20041 cctaccgaaa caatgacagc ttttacagaa gatgaacaag aaataaatag ttatctagaa
    20101 cgtgggcatg aattattaaa agaaaaagaa gtagcagatt tgccattttc tctgagtcta
    20161 gatatcttca atgagcattc gtttatccaa gtagcctttg aggcggtgaa agaacgagga
    20221 aagacggaag cagcaatatt aaacagtggt ctgtttttaa ctgaaattcc aaaagggcgg
    20281 gtcaatcagg atcagcttca cactgcgttg ccgcatccga tgcacttgtt aaatgtcaca
    20341 ttgggtggaa atgatctgat ccgcttagtt ttagaaatag aaaaaaatcg taacttttta
    20401 cgtaactatc ctatgaaggg aatgggattt cgcggtaaga tttttgggca gatggtctat
    20461 aatggaatca catatgattc tattaaccat caagtctttt ggaagaacaa gccaatcgat
    20521 ctgaataaaa attatacttt tactaccgta gatcatttga tgtttgttcc attttttcca
    20581 acgattgaga ttgctggaaa aaatgaattt ttgttccctg aatttatccg aagtgtggta
    20641 ggagattact tgaaagctca ctacccaatc aaataaaaac aaggtataat aaaaaagata
    20701 ggtggtgaac gcatgtctgc aaaacaaaat gaacagcaga acgttgagca taaaacagaa
    20761 attgctaaga aaaaatcatc agctgaacag gaaacaacag tcaccgaaga actgacagct
    20821 gctttggaag aaatcgttga ggagcctaaa gcagaaaaag tcaaaggcga gctagtgaca
    20881 ctgttagatg acagtaattt tttgataggt gaaagacatt atcggcttgt gtccgattat
    20941 cgtgaaggat ttaatgcaga aaagcttgga gaacgttaca gtgatgtact agcccgatat
    21001 gattatatcg taggagattg gggttatgaa caactacgcc taaaaggatt tttccaagcg
    21061 gataatcgac gggcgcatcc agatcagcga atcgattcct tggaagacta tctttacgaa
    21121 tattgcaatt ttggttgtgc gtattttgtc atcgagagaa tcggcggtaa aaaagaaaag
    21181 acaactcagc gccgaaaaaa gaaaaaaaac cataatcgtg cgtatatcga tgagaaaaaa
    21241 ggaccagtcg catctaatcg caaaaaaccg gttattaaaa atcgaaaaac agagtcagca
    21301 aaaacctctg aaaaagtaaa aaaacaagaa caacctaaac aagtagaaaa aacaaaaaca
    21361 gcatttacga tcagaaaaag ggaagagtag tggatgaatg gactataaag gatatttaat
    21421 tgatttagac ggaacgattt atcgtgggac acaacccata ccggccggta aacgatttgt
    21481 ggaagaattg caaaaaagaa aattaccttt cttgtttgtt accaataaca cgactaagtc
    21541 accagaaaca gtagctaatc gtctagcaga tgagtttgac attcatgtag ctccagagac
    21601 agtctacaca gctactttgg caacaatcga ttttatgaaa gcagatggaa aagggaaaaa
    21661 agtctatgtt atcggagaag caggattgat tgacttgatt ttagctgccg gatttacatg
    21721 ggaagaagag gctcctgact atgtagtggt aggcttagat aattatctga cctatgaaaa
    21781 agtagttaaa gcaacacttg ctattcaaaa aggtgctact ttcattggaa cgaacccaga
    21841 taagaatata cctactgaac gcggccttct tcctggagca gggtcagtca tctcttttgt
    21901 agagacagca acccaaacgc cgccgatcta catcggaaaa ccagaagcca tcattatgga
    21961 taaagccgtt gaagtattag ggctgcaaaa agaagaagta atcatggttg gagataacta
    22021 tgaaacagat atccaagctg ggatacgcaa taatatagat actttgctcg ttttatcagg
    22081 ttttacaaaa gaagaagatg tacctgggtt gcctatccca gctacctatg tgaaacaatc
    22141 actggatgaa tggagcttct aatatgaaaa attcgagatg gcagtggatg gaatatgctg
    22201 gattgttcag cctatttctg acactgatca gtctggcagt tggtgtgacg atcaactttc
    22261 gaccactata cgtatttgat atcggacatt tgcaaatttt ggattatact tctttagatc
    22321 aagagacgtt actgaaaaat tttgatcacc taatgaatta tctcaataat cccttcaaaa
    22381 ccatcctcag cttgccagat ttcccagttt ctgctagcgg agcccatcac ttttatgaag
    22441 tgaaaatttt gtttttagtg gattatgctg tgtttttcat cactttgatc ccgagtattt
    22501 tgtttatcaa atatttgcaa aagaatgatc ggctttggcg gttgattcgt ccgtttcaaa
    22561 tcggcatgtt actgccagtt gttttcggtt ttttcatgat gataggattt gatcgctttt
    22621 ttatcctttt tcatgaaacg tttttcaaca acgatgactg gttattcgat cccgttactg
    22681 atccaatcat caatgtactt ccagaagaat ttttcatgca tagctttatc ttattttttg
    22741 tattattaga attatttttt gctgtttttc ttttcttagg aaaaaatagc ttgaaacaaa
    22801 caaaaaagaa agaactcgtc taaatagacg ttttctttct ttttttgttg aattattgag
    22861 taaggcgact tcttttttct ggttctttgt gtgctaatcg actggcagca gcagcggcga
    22921 tcgctccgac gatatcatct aagaaggtat ggacatgaac gccatcgtgg ctgtttagtt
    22981 cttttaaaat accaggtttc accttgtcga tataaccata attcgtaaag ccgatcgtac
    23041 cgtaaacatt cacgattgac aaggctagga tctcatcgat accgtaaagg ccttcgtcat
    23101 cttcgataat atgttgcaac ggactcatca attttttctt ttctgctaat atatctaatt
    23161 ggattccagt gataatggcg ttatgtactt cacgctttgt tagcacgctg ttgacacttt
    23221 cagtacaatc cgccaaagtc aaatcagaaa tatagtcttt ttgtaaaaac atgacaagtt
    23281 gtgcgatatc ttctacagtt acgccacgtt ccttcaatag ttctcttgcc ttttcttcta
    23341 aagtctcagt tttgacgacc atattattgc tcccccttta ttttttcttt ctctatttca
    23401 tgttaacgga acatgaaaaa aatgcaagaa actgctttcg ggtttaactt gaaatgcaaa
    23461 gagatataaa aaatcaacta ggacgaagat ttttatcgta taagcttgtg ctgtgaccag
    23521 gctgagttcg ttcctttgga ttaatatagt gtaaagcgtt gttcactgca gtaggtgctt
    23581 caccgagccc agtagcaatc agtttcactt ttccttcata ggtacagatg tcaccagctg
    23641 cataaacgcc tggaatagaa gtagacatat ctgattttac agtaatggca tttctggtac
    23701 tatcaagtcc ccaagaagaa agatgttcta aattagaaga gaatccataa ttgacgatca
    23761 gagaatcaat catcaaatca atcgtctcgt cacttttcac tttttttaac cggatatctg
    23821 tcagttcccc attattgcct gataatccat cgatgagata gggagtaagc aagttgacag
    23881 atgaagattt caagcgtgat acactatgtt cgtgcgcacg aaattccggg cgacgatgaa
    23941 tcaagtaaac ttcgcttgca ataggctcta acatcaacgt ccaatcgata gcagaatcac
    24001 cacctccagc aatagcgact ttttttccag catacttcat caaatcattc acgaaataat
    24061 ccaatccgtg gttttcaaat gattcagcat tatctaaatt caattttctt ggctggaatg
    24121 aaccatttcc taaagctaaa atgactgctt tggaataatg gatgccttta tttgtagtaa
    24181 tctcgataac ttcgtcttca cgagtaagcg agagaacttc ttcttttaaa tggaatgtat
    24241 ggttaaaagt agttaattgt ttttctaatt gatcgattaa ttcacttgct tttattgctg
    24301 gatacccagg aatatcgtaa atatattttt cgggatacaa agttgctaat tgaccaccaa
    24361 gttgaggtag actgtcaatg atttttgttt tagcttggcg cattcctgcg taaaaagcgg
    24421 caaacatccc tacaggacct gcaccgatga ttgtaatgtc atagatttcc ataaaaattc
    24481 ctcccttttc acactatagc agaatttaat tgagaatgct tctcaaaaat gtttgaattc
    24541 gaaatcgaaa cggtaagtta tagaatgtat ctagtgatga aaaataaaag aaaacaaatc
    24601 tagtattttt acgagaggtt aggtattatg ttattattat tatgaagtat gcaaat
//
LOCUS       DAYYGS010000040        23800 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000040 DAYYGS010000000
VERSION     DAYYGS010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.040"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            370..1809
                     /locus_tag="ACHA44_001492"
     CDS             370..1809
                     /locus_tag="ACHA44_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8904774.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            2104..2322
                     /locus_tag="ACHA44_001493"
                     /pseudo
     CDS             2104..2322
                     /locus_tag="ACHA44_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            2571..2786
                     /locus_tag="ACHA44_001494"
     CDS             2571..2786
                     /locus_tag="ACHA44_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904775.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(3003..3259)
                     /locus_tag="ACHA44_001495"
                     /pseudo
     CDS             complement(3003..3259)
                     /locus_tag="ACHA44_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3446..4357)
                     /locus_tag="ACHA44_001496"
     CDS             complement(3446..4357)
                     /locus_tag="ACHA44_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8904776.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(4376..5380)
                     /locus_tag="ACHA44_001497"
     CDS             complement(4376..5380)
                     /locus_tag="ACHA44_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HFM8904777.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(5377..7500)
                     /locus_tag="ACHA44_001498"
     CDS             complement(5377..7500)
                     /locus_tag="ACHA44_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD3337/EF1877 family mobilome membrane protein"
                     /protein_id="HFM8904778.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(7505..9952)
                     /locus_tag="ACHA44_001499"
     CDS             complement(7505..9952)
                     /locus_tag="ACHA44_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8904779.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(9939..10328)
                     /locus_tag="ACHA44_001500"
     CDS             complement(9939..10328)
                     /locus_tag="ACHA44_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFM8904780.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(10388..10891)
                     /locus_tag="ACHA44_001501"
     CDS             complement(10388..10891)
                     /locus_tag="ACHA44_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFM8904781.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(10904..11128)
                     /locus_tag="ACHA44_001502"
     CDS             complement(10904..11128)
                     /locus_tag="ACHA44_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904782.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(11232..13142)
                     /gene="ltrA"
                     /locus_tag="ACHA44_001503"
     CDS             complement(11232..13142)
                     /gene="ltrA"
                     /locus_tag="ACHA44_001503"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8904783.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            complement(13888..14025)
                     /locus_tag="ACHA44_001504"
     CDS             complement(13888..14025)
                     /locus_tag="ACHA44_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HFM8904784.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(14022..15206)
                     /gene="mobT"
                     /locus_tag="ACHA44_001505"
     CDS             complement(14022..15206)
                     /gene="mobT"
                     /locus_tag="ACHA44_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobT family relaxase"
                     /protein_id="HFM8904785.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(15465..15755)
                     /locus_tag="ACHA44_001506"
     CDS             complement(15465..15755)
                     /locus_tag="ACHA44_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904786.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(15745..16887)
                     /gene="dcm"
                     /locus_tag="ACHA44_001507"
                     /pseudo
     CDS             complement(15745..16887)
                     /gene="dcm"
                     /locus_tag="ACHA44_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(16963..17127)
                     /locus_tag="ACHA44_001508"
     CDS             complement(16963..17127)
                     /locus_tag="ACHA44_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904787.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(17120..17794)
                     /locus_tag="ACHA44_001509"
     CDS             complement(17120..17794)
                     /locus_tag="ACHA44_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8904788.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(17872..19212)
                     /locus_tag="ACHA44_001510"
     CDS             complement(17872..19212)
                     /locus_tag="ACHA44_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HFM8904789.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(19295..19630)
                     /locus_tag="ACHA44_001511"
     CDS             complement(19295..19630)
                     /locus_tag="ACHA44_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HFM8904790.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(19640..20014)
                     /locus_tag="ACHA44_001512"
     CDS             complement(19640..20014)
                     /locus_tag="ACHA44_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8904791.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(20027..20341)
                     /locus_tag="ACHA44_001513"
     CDS             complement(20027..20341)
                     /locus_tag="ACHA44_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HFM8904792.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(20454..23681)
                     /gene="fss3"
                     /locus_tag="ACHA44_001514"
     CDS             complement(20454..23681)
                     /gene="fss3"
                     /locus_tag="ACHA44_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HFM8904793.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         6654 a         5140 c         4110 g         7896 t
ORIGIN      
        1 tggaattgaa tatcatagca atgtatttga aaagcaaatt ggaaaacaaa taggtgcaac
       61 accataaata tgaaaaaatt gggaaaaagt agaatggaaa tttgcttacg tctcacgaga
      121 tagctcacac tattaatctg aagaacaatg aagtcgactt ttagaaattg ctagaagaaa
      181 gtaaagcgaa ttatcactag ttactataga gtaagttcaa ataaagcatc tccaacttac
      241 atggttacat gaggatatca aatcgaaatt actcaagctt cctatggcta gagtcgcgag
      301 aagttgttgg gcagtagaca taggttatat tgttcttagc tacacgatca ttagaaagga
      361 aatcctatta tgtatagtta tcaagaagat gcaaaagtcc aaaaaaatcg ctggtggatt
      421 ttgattgcta tatctatgtt taccttcatg tccacattgg atggcagtat tgtcaatatt
      481 gcactaccta ccatctcaga tgatatgggc ataccaatga atcaagcgga atgggtagtg
      541 tccgtttatc tgatgattgt ctgttcttgt ttgttgctgt ttggtaaaat tggcgatagc
      601 cgtgggaaaa tcaaagtatt tcggattggt acgctgatct ttgtcttggg atcgctgttg
      661 tgtgggttca atcattcgct aggtatttta ttgttcgcac gggtggtaca agccattggt
      721 gctagtatga ctatggctac aaatgccgga atcatcacgg aagtctttcc catacatgaa
      781 cgtggtcggg cattgggagc aataggtgcg tttgtttcat taggctcgat tgctggacca
      841 ggtcttggcg gcttgatttt gtcccaattt tcttgggcgt acattttttg gatcaatgta
      901 ccggtgggaa tcgtaacgat gttgattgct gaaaaattct taccaaaaga tatcaccatg
      961 agcggccata caatcgatgg agggggcttt gctgccattg cggctacgat tatcagcttt
     1021 tttggagcaa tctttttggg gcaagaaagt ggctttttcc atccagtccc ccttgtactg
     1081 ttcgtaattt cagtgattgc ctttgtcagc tttttaaaag ttgaaaagaa agcagcctct
     1141 ccattgattt ctttcggtat ttttaagaac aagattttta ccatgagctt gattacagcc
     1201 ttgttgattt tttcttccaa cttctttgtc aatgtagtga ttccttttta tctgcaaaat
     1261 gctcgcgggt tgagtcctag ctatgctggg ctgttgatga tggtctttcc tttgatgatg
     1321 gtcgtcgggg cgcctttaag cggctacctg accgaccggg ttggcccgga gattttggta
     1381 ttagtaggct tgggcttatt gtcggtaacc caggtcatgt atatgttttt gtcagtggac
     1441 tctcctttgt ggctatacat tgtcgctact gccatcatgg gtgccggcaa tgccttgttc
     1501 cagtccccca ataatacgat ggttatgagc tcggtcacta tcgaaaactt gggactggca
     1561 ggctcgatga attcctttgc ccgaaatttg gggatggtca gcgggatttc gctttctacg
     1621 acggtcttat atcaagcgat gtctgctaag atgggccagc aagtaactaa ctatatcgca
     1681 aatcggccgg atgtcttttt atatggaatg aagattacct ttttaggcag ctttgttttg
     1741 tgttttgttg cctttttcct gactatctgg cgtatcaaaa aacaaggagg tattcagaaa
     1801 aatgtctgaa aaaaccactg ttgaagaatt tttggaggcc tgcgtatggg tgagtgggat
     1861 ttccatggat gatggaagat gaagtgaact tctaaaagcc gaaaaactca agtattatct
     1921 tcaaaatact agtgctcaat gagatgcttg atttgatcac aagaagactg cgaaagccgc
     1981 tggcaaccgc aagctaggta tcccaccgat ggactacgtt ttagaggcta ctggttatga
     2041 atacggagcc aacgctctca tctgcataag aatacatcac ccagattatg taaagatttt
     2101 cgatacttct gtcaaaaaat cattaagata gataaatgat ttccgtctag tcaaattggt
     2161 tcagaatgta gacacacaga tggtaagaag tcactcacaa ttcgagaatg gacttgtcct
     2221 atttgccgta tacatcataa tcgaaacgtc aacgatagta tcaatatttt gaccaagggc
     2281 tatgaataca agcattgcct tagaaaacat caagaacctt aggaacaacg gggatagctt
     2341 ggttaataag agataccgct gtttaggctt gcaagtatac tcttttatca agaattccgt
     2401 ggctttagtc atgagaggct taatactatg aaaattttct ttagcatttt tgatttaggt
     2461 tccttttcta attataataa gatgaccact acagtaacct actgaaatat ataaacagcc
     2521 actgtagtac cattttcaaa gtagtctatg agaggagaat cttcattctt atgaacgctg
     2581 gaattgcgga tatgaattta ataaaaaaaa cccttaatga ttttacttcc aattcaatat
     2641 caaaaggtac tggtatcaat ttaagcacaa taaaaaagct gaaaagtggt gaaagatcag
     2701 tggaaaagct gaatctttta gatgctataa aaataacgga atttgctatg aaaaatggaa
     2761 aagctgagat tgaaatatgg agataattat cgtctagtga attacttgcc acttagctga
     2821 atttaacgat ccttggtgct tgaaggggga gattcttggg aatagagcat acttgcaagc
     2881 ctatgtcttt actaacaacg atatctctta ttaaccaagc tatccccgta gttctaaggc
     2941 ttttactttt atctaagcaa taatcgtatt caaaggaaat ttttgaccct tagaaagtct
     3001 cattttatca gacgtgagct taggcattgc tgaattttgt taattcggtt ttaggttgtg
     3061 ctttgagcat aatatgaaag tattctttat cacgatttca ttcatctaag gtaatgtgat
     3121 gtgattctga aattctttca aaggcagttt ttgcataagc tgacattaca tcatcaatga
     3181 catgtttaca atgtttcact actaaaataa gggggtaacc aagaagaaaa actgaatgat
     3241 tatttgtatc taattccatg gtatatcaac catttcctat atagactgat tatgacatgg
     3301 attaaaataa aaaggcacta tgtcttaatt gtcggttttc gagttaccga atggcattca
     3361 tcacccacct atagaggatg ggcgacttct gcctgaaata agttacacta cttataactt
     3421 ctaataaaat tagattgagt cattattatt taacatcaac gattttccag tttccatttt
     3481 tttgtaatac taatttgtac tgaaagtagc tagtggtttt tgatacatta tcaaggtatt
     3541 ttatctttgt actcactaga aaattttttc cgttttgtag ataaactgga ttctctaact
     3601 ccgaaaactt taagtcaata ttgattggtc gcatcgcacc attttcaaca taatactcca
     3661 attctttttc agaagctgat ggataaagtt taaaaaatgt ttctaaaaaa tcggtgattt
     3721 cttccgttgt tttggcatca atttctgaat cgttttctac ttgttttgct tcatattttg
     3781 ccttgtctgg ctttccaatc attgttggca aactagttac taaatgattc ctgttttcat
     3841 cttcaaagat tgtcactcga tatgcagaag aaattgattg cgtctttttt ccctctgtaa
     3901 tatgttgctc cactaaaaat gaaacgacaa attcttccga gtgtttttca acatctagaa
     3961 tttttacaga ttgaacttcg gaactcgtgg gaatatctgc tcgaaccata tcttggatta
     4021 aagcgagacc gttttctgta aggtactgtt cgagattact cattctcttt tcaattgctt
     4081 ctttgttatt tttccatgaa aaatattctt tcgcaaaatc ctctgtaaaa ctttcaacgc
     4141 cagaagtatt cactaccttc tcttttataa ccactttttc atgagttgta tgttgatcaa
     4201 tggcggtgaa atttttataa acgccaaaag caagactaca agacaataaa agccaaaggg
     4261 caagcaccat ttttcgatgc ttacccacgg atacaactcg ttcttttttc ttttttgggc
     4321 tcttttcctt ttgattccgt tcaattttta tcttcattct ttttcctctt ttctcttatt
     4381 tgtatcggcc agctccgata agatgctgcc gccaatagtt ttccgttaga ttcgtccacc
     4441 ccactggatc tcctgcatga aacatgcgat tctcccctgc gtaaatgccc acatgggtca
     4501 cataagtacc ggcatcatag gttgaatgaa agaaaactaa atcacctggt ttggcatctt
     4561 ttagatcaat atgagtcatg gcatcatatt gatcctgagc cacccgagga agtttcaggt
     4621 caactttggc ataacaccat tgggtcaagc cactacaatc aaaccccgtt tctggacttg
     4681 agccaccaaa cacataggcc gttccttcat acttaaatgc ctcatcaaaa atttcttgga
     4741 ctattttgtc atcaaactga gttgtagtta ggtattgctt cactaataaa cagtaaaaca
     4801 tattgccata attataccgc cacccaccat tttccttaat cgcgattggg ttggggtaat
     4861 cgactttttg accacctgat ttttcctttg aaaaagattc agctaaagca aaactatgct
     4921 tcttcccgtt tttcccgaca tgatctagat acccgccacc atagttatag gcctgaataa
     4981 tactatcttg atcacaccct agttctttgg aacgcttaac caaagacgca aaatagagac
     5041 agccttgttt gatggatgcc tcggtactta atgaattagg tggtaatccg gctgactcag
     5101 aactttgcat aacgtcttcc attgtgccac cagattccac ttgaataatc gccaaaagaa
     5161 ttggaatata ctctgttata tccgattctt tggcatactt ttccaccatt gcacggtgtt
     5221 tcaatacttc ttctgagaca ctagttccgc caggagaagt gccagaacta ccatttccct
     5281 cactgtcttc accaaacaac aaacccacac acatcaaaat acccataaaa gagatggtca
     5341 gaaaagttaa tgaaataaca cccactttct tcaagttcat tttttctcac ctgaccttga
     5401 ttttggacga tggttcattc ttttttgtac tttttgattg gtctgctttg gcagagtgga
     5461 cctctgtttc acttgttgga aagaattttt cttacttgct gattggcttt tcccttttga
     5521 aatgatttct tgactagtta gttttcgttt gatttcggga ttcagatttg ctctcacaat
     5581 ccgctttggt gcttcttttt tcagcatcat atctttctga ggttgagctg ctttatctat
     5641 tggaatattc gcccttcttt ttccctgacg atttgttgct tcactcagta tcttgcgttt
     5701 ttcgtccatt ttcgcatttc ttcgttgtcg ttgtttttct cgcaaatcca ctcgatccgc
     5761 tttttcttgg aacagtccgc gcttgaattc ttctggtgct tttttcgact tttcgattcc
     5821 tttatggaga ttgtatttca aattcgttgg cgtattcctt acctgatcct ttacttgttt
     5881 agtcttatct ttcaccaaag cttgggtatc caaaactttg ccgatttttc tcccagccaa
     5941 attcattcgg gattgtttct tattatttga aactggattc tccttagtta agtcagatag
     6001 tatttcgtga tcgttcggta atcgtgaatt ctttcgtaga gaagaaccgg atggcttaaa
     6061 tttggatttt gattgttcct ttgccaactt atttccgact gctgcaccac caccaaaagc
     6121 caaagctcgt cccacatttc gattgagttg ccgcaatttt cgattcatta acatttgcgg
     6181 ttttcgcatg acgcgtcttc ccattgactg gctatcatta ctctggaggt tgaacatact
     6241 cataatgtcg cccaatttga aatagatacc ggcaaaggtc acaatttgca gaaaagcaac
     6301 catgaaaaat ggataggttg ccgaaatatt gaaaaacatc gtggagatgc taaatgcagt
     6361 cgtaatcacc aaagtaacac ccgctcgcat cataatcgta ttgaataaac gaataatcgc
     6421 cttctttcct aaactttcat aagtcggcaa catggacaat aagaaactaa ttggcagaaa
     6481 catcgcaaag ataatgaaga gaatttgcga gaacaacata atccctgtta gaagaaaaac
     6541 aaagaaggaa atgccaatat tgaacaatac caaaaagacc acggtcccta aacggttcat
     6601 ggtcttggta atactgagat tcgcattgtc attgtcttca atctctgcct tgaccgcttc
     6661 ttcccgatct ttgcctttgt tttcatctgg actcacagat agaatcttgt tgacccggtc
     6721 ttcaccaatt tcttctatgt tggaatcatc aaattgcagt agtaaccaag gttgttggac
     6781 ttgaatcgaa aacaaactat cccgaatcaa atccacacta tcttttcctt ggctttctga
     6841 attgggtaca acgatctcgg ttccaagggt aagggccgct tcactaacat ctgagctaaa
     6901 gtcattgatt tttgtgatat aggtaggcgc ataagcaatg aacgaacccg atagtagaaa
     6961 aatcatcaca aaattaatca cggctcgaac tgctttcgtg gtttctcgtt ttaataatcc
     7021 ggtatacgcc acatacaccc ccagagctaa aatcatcaat aatagaaacc caaaataaaa
     7081 tccggaggtc tggagtccat tttcagtaat accagccaat gtttggatat tctttccaat
     7141 actttcagcc gtatccgaaa tgaaatctaa tttgtaagcc tcttgaacca cataacccgt
     7201 ggcgttcgac aaatacaaac tcaccgtcca aatgaaatta gtaatggcat aaagaccata
     7261 catcacgctt ttccctaagc catcacccca attccaaggc aaccaatccc aagagctatc
     7321 cacaaagaaa tctagttgat agttgttcag tgaatatttg gaatatagat ttcctgcttc
     7381 aactgtatca tcaactagcc ctgtcgcctc ggctaccgta ccaactaaac tcaatagaac
     7441 tatcagagtg agtatgctaa taaaaacgat tcccacaatc cgaaaaagtt tctttttcat
     7501 cccctcactc cattctctct tgaacaggtg gacgggtatc aaaggcatgg aagagttctt
     7561 caaagactgg atgtacctga accacgccta cccgaccata caaatcctgc atcaagcatt
     7621 ggccattttc caaatctcgt aggcgttttt gattgctttc atcttctcga tccaagccaa
     7681 agaattccaa ggttttcttg atttcatgaa tatcggtact gcgaaacgca aacttcaacc
     7741 cgatattatt cttcaagcgt tcatccagta aatcatcact attttgcgtg acaaagtaaa
     7801 ccccggcatt catagcccgc ccagcacgaa cgagcttatt cgataatgtt ttcccttgcg
     7861 ctacttgaag aaaactccac gcctcatcta agtctacaat tttgaagatt tctcgattac
     7921 tatggataaa gtccaaggca aaagtggaaa tgacgattag catggaaaca ctaagcaatt
     7981 ccatggtggt atattcttca aaagtggttt ctgcatctgg taaaaccaaa tccgctactt
     8041 gaatgatatt taactgccta tctaaactaa tcgactgggt aatcgaacca tctgagaaaa
     8101 gcaaatgggc aaaatcataa tcggtaaagg actcgatgtg atccgcgata ttttccgcaa
     8161 ctggtgagcc ttcttttcga agctcattaa ttacttgtaa taatccccgt tgattgcttt
     8221 gggtaaccgt ccgaatggcc ttacgcaaca ctggaaactt ctccccatct cgactagaaa
     8281 ttcctgttaa aaaggtcaga atatcaattg ctagactttc agaatccttc tttttctcca
     8341 taatcacata aggatcaagt aatccttgat tttttggctt actagttaag ttgagaatat
     8401 tgatttcatc cgcaatttct ggcaaggttt ctttccagcc accccgttcc ccttttggat
     8461 cgacaatcac agcttgtcca ccaaaaagga cggcgtaata aactaagaga ttgttactaa
     8521 aggactttcc gccaccgagt gaaccgagga atgccgctgc tagcgcattg gtaactgaat
     8581 ccttgacccc ttgtgaggcc aatgccggtt tcaggtaaac attgcgcccc gtatctacgt
     8641 tataaccaaa gtaaatgcct tccaactccc ctaacatctg agtggcgcca aaaccaagtc
     8701 cggcgagaaa atcagacgta acatactgga tataatcatt catgtatcgc ttactagaag
     8761 gtaagaattc ttcatgtagc cccatcatat cgccaaaagg ccggactagt tttacattgg
     8821 tatcatcata aaaatctagc acttcatcac accggcgctt tagttcatct actgattcgg
     8881 cacttactcg aaccacatag ctcagtttgt acatggattc tttggactga tccaaggtag
     8941 tttccaattc atctacggaa tccaatgcat ctaagacatt ggaatttgtt tcattgtcag
     9001 attgataggc atggttgtcc aagtctttta attccttttt cttattgcga acggtagcaa
     9061 gtgccttttt gttcgtcaca atctccacat tcatactagt gtcaattggg aatgtaaatt
     9121 gttgttgctg gtaatagaaa agttctgagg agggaaactc catttccccc acaattgtat
     9181 taattgttaa gtacgctaca tatgattcat gattctcatg ttccatgcat aagtaccggg
     9241 gcttttcttc aatcaaacaa cgactgggac gtaataagtc gtaacgtttc accaaagttt
     9301 ccgacttcag ctttttcttt ggtagctgaa attcatattc ttcaaatggt gtaccctttt
     9361 cgccatagat atgttcaatc aaatacatca gatcactcgg tgtgactcgt ctgactttaa
     9421 atcttcgagc caatttactt tccattagtt tttcaagctt ggtatatcgg cgaatttctt
     9481 cattggataa ggaaacaaaa tcgcccatca aatgatgatt cactccatag acaaattctt
     9541 gaaagccgga gaagaaagat ttcttgagat tctttagatt gacctcttca tctgtcgcaa
     9601 tcagtttgaa gccaataaaa aagcgataat caatctgact atcgccaatc atggatacta
     9661 atgcctctgt ctgtaaatca attcgttgtt tcgctacttc tgttaatcgc ccggtaattt
     9721 ccttttttga tcgttcttgc gtggcacgaa tactactttc tgtggcaatt tgtaaggcat
     9781 gaatcttgcc ttcacgattt tgcgcaatca attgacgaaa gttatcgtgg acttgatatt
     9841 tctgttcagg tgacaaaaag gagtaattgt aaggtattaa ctcatagtag gcaaaacatt
     9901 ctccctcttg attaaagaca agattatttt caatatactt aatcgggtac atagttcaca
     9961 ctccttacca gagtaatggg ttcgtcccat cgtttcttct gttctttgac tttctttcct
    10021 gcgaaagtga ccttaaatcg taagtgatag ctaataaaag atcgtaaaaa tcccaatggt
    10081 ttcttaccgt caaacgtttt ttgcgacatg aaccaagtaa ctgccaccgg tatacctaag
    10141 tattttaaaa aggccccctc tatcatagat agtggaggca catgggcaaa tacgatgaca
    10201 aacagcaagg ataaaacaaa ccaagccatc tggttaaaag tcacaggaaa aggcagttga
    10261 aagtcattaa ttgcataaat gaccttttca actgcccaaa tactggtgta gctttttatt
    10321 ttcttcatca aatcattcct ttaaaaagaa ggatagttta gaaatcaact acccttcaat
    10381 aattttgtta atatggatac tcaaaaacac ctcgactcgt taccaaataa ttgcctccaa
    10441 tctccaagtc tctaccacac gcatcaaaat caatatacgt cttcaagtgt tctggtactt
    10501 ctccaaaatc accagcttct acaatcaaga aatatgctac atcactcata tcatcgcaat
    10561 ctggataata gataatatca tccacatgct ctaccatctc ctcaaaagaa ttgaagaaag
    10621 catgttgaat ctcagaagca acttcaccaa ctggcgttcc ttcaagttcc tccgctaagc
    10681 cacacaaccg attaatttcc tcaattggcg tatactcatc aatctcaaag ggaagttcat
    10741 aatcatgaat tgcatattcc tcgtattcgt catttaaccc aattcgttct ttcacttcat
    10801 caaaatcgac aggtggggta aaccacgccc ccaccaattc accttcgtta tatttcccga
    10861 ggttcgcaat ataaacacgc atctgttcca tcgtgcttcc cccttacgca ccgacaattc
    10921 ggttaaacaa actgagtaag acatccttca ccccagaagc attaaagacc aatccgaccg
    10981 caatcaaagc aatcaccaaa aagccaatca gtttggaaaa ctcccgctta aatcctaaaa
    11041 acaaaccaat gaccacaatt gccagtaaga ctaaggattg ggcattggtt aaaaaccatt
    11101 gatatatgga taggtaagtt cctgatatta taaatatctc aaacttttcc ccccattcac
    11161 acggagcgtg cgactttcac cgcactccgc gttccatcgt gtttatattg aaaagtattt
    11221 atgtagtaga cctatatcaa gtttaattct tgattgccag ctaatactct atatttattt
    11281 agtcttatga gctggacttt atttagcttc aatagagcta tatatttttg tattgtttct
    11341 tctgttttag cgtgtattag acggtgtacc gcctctttta ccaaaataag atttttataa
    11401 tcgtcatttc caccagctcc agttggtaca atatgatgac aatgtatttc attagcttgt
    11461 tgaaattctt ccagagttat agcacatttt ccatgttgag cagaaaatag cgaaattcga
    11521 ttgtctgcgt attctattgt gcgattttcc aaagataatt tcattaactg gtacatcagt
    11581 aatttatcaa cacttagatt ggtgtggata aattttcttc cctctagagt gtagcaattc
    11641 gctaaataca cctttgccat tggttttctg tgttggatat atccaacagg gaaaataggt
    11701 tcgccctcgc cagcaacata ccgaagcatt ttagatttac catatcttgt tgcctccttt
    11761 gtcgtaagtt gtctgcctgt ttttctcaat ctgccccttt tgtttgtacc taatctgtta
    11821 gtgaagatag ccattacagc tctattcagt ttcatacaat ccagatttac attcgttgca
    11881 atctgataat aattttgcat acctaatacc attgaattat aaagtcttgt ttcttctaat
    11941 tccgttttcc cttttgaggg agaagcaatt ttctttgcct gtgataccag cttgtctttt
    12001 tggtttttga attgtttatc acttatatgt gatttaacaa ctaacttctt tcttctcggt
    12061 atcattttaa tcttgaaacc aagaaagtcg gagtatcttt ttctaacatt tacaattctt
    12121 gttttttctt gtgatacttc caattttaat cgttgttcaa tccattgagt gattgctatt
    12181 ttggcttttt cagcactttc cttgtagcgg cagaaaactc taaaatcatc tgcataacga
    12241 accatataca tttcctttaa ctttgttttc ttcatttctt tgtaagcgtt gcttttacat
    12301 gggctaccgc ttttattgat atgaaccaca tagttttttg taacaggatt ttcctgccat
    12361 tggctttcta cccaatggtc taactcatta agaacaatgt ttgctagtag tggggatatt
    12421 attccccctt gcggagttcc tttctctgga tatgttattg taccgttagt cattttgatt
    12481 ggagctttta atatcctttt taaaatgaat atcaagtgct tgtcccgtat tcccatagcc
    12541 catatctgtt ttatcagctt ggaatggttc acgttgtcaa agaaaccctt aatatcaaat
    12601 tcaatcacat aatgtagctg gctgatttgt aggcggttat aggttgcttt tattgcattt
    12661 tcaactgacc tattaggtct aaaaccatag ctgttttcac taaatttagc ttcgcagatt
    12721 ggttctaata cttgttttat acattgttgt ataagcctgt cccacataca aggaatacct
    12781 agaggtctgg ttttaccatt tggttttggt atttcttttc gcctcactgg cttaggtcga
    12841 tacccatgtg gactgcctgc aacaatgtat cttacttttt tgacaactgt ttctgctggc
    12901 aatttgccaa tatctttaat tattgtaccg tctgttcctg ctgttttact gcctgcattt
    12961 gctttgatat ttcgatatgc cagtaatata ttttctctcg ataggataat atccataaga
    13021 tgagtgaaaa tatctccatt cttacttttg gcatataaat catcaaaggt gttttgcatt
    13081 tcgtaatatt cagcgtgtct taaatcttct acacacaatg tttcattttt atttttaggc
    13141 ataaggcttc actcctttac ttgaaattga gtgagctttt ttatcttact cgtaatcctt
    13201 attgaaacaa cataatactt ttctaatata tacacgactt gagggcattc ctccattctc
    13261 attacaagaa tttcttaggt tcgccctctg cttttcagca ttagttcaac tgcttattgt
    13321 tcttcgtcag tttattctct tgatgagaac ccaatacctt tcctcgttcc gataatctta
    13381 tctatacata tatatcctta ggtcactagc tataagcctg ctggcttgat accgcctgta
    13441 acgttatatg gcatttcata acctatattc ttactttgcc acaccagcta cgatttctcg
    13501 tacctttgca tttctacaaa gttcaaattt agacccgtac attcgctgtt tcgtcagttc
    13561 cttatagaac attctaacca tagatatttt atagaccccc gacctgttgg atacgctacc
    13621 cacctctgga tagttttcga tagaagatat atgttcatca actattacgg tatctttcag
    13681 ccgtctttac cgagcttcaa acattctgag tttcctcata caatgcttgt cggaggctca
    13741 gggcaggcgt ttcagagcgt tacttcatca ttccacctgt cagattatca gttctcctaa
    13801 taaccaaact ttttcgttct tttattagtg ccttagcttt tcactaagga acgagtcgca
    13861 cataaatttt gtccgaaatt catcttgtca ctttcctttc ttttccattg ctcgatccgc
    13921 tgctgcactt gcattcatca agcacatcac agtaacacca agcatactgc caccaaatac
    13981 caaaaaaata tcttttacaa ttcccatgac gattcctctt ttcatataat caaattttca
    14041 attgccatac tttgttgctc caaaatcttt ttatgtcggt ctgataagcg tgcattctgt
    14101 accatatcct ggatataggt tgtccgattc aatcgatcca actccttagc cattttccac
    14161 gtaggggcga cttgtcggcc tagccaattc aaggttcgtt caatggtata cggttttggc
    14221 tcggaagtta attgaatctc tttccgattc cgtccaataa aacgccccca ttgttcattc
    14281 aagggccagt tggttcgcct tttaccttct actttatccg caaatcgcaa gtaacgattg
    14341 ataatggaaa acgtggtgct ctcaatgcta cgacctttta acaaatcttg aatcgcatga
    14401 aaagcacggt catttttcag tcgaatctca aaacgatttt tcgtctcagt ttggtctaat
    14461 ggaatcccta acttgatata ttgttcatag tctttttcat acaaacagaa ataaacctca
    14521 ctcttgagac tgccgatata taaggtattg cccatgccgt ccttttcatc agcctttaaa
    14581 agttccccgg aacgataact tttaaaggta cgaaacaagg agatacattc ttccttctga
    14641 cattttttcg ttagctcagg tatatccaat agccccactc gatcattgat tgccaagtcc
    14701 aagcgtttca tcacgccacc ggcctttaga caatcctcga aaaagtcgta ccagctacgc
    14761 ttttgagcga gtaaaaaagt ttcaaattgt cggcaacctc gcccctttaa ttctaaaagc
    14821 acacctttat cttcttcatg agacaacatc accacaatat cacccatgac atactgttct
    14881 tgataggagt aaaaggcata atcttcatgg agcatatact caaatttcaa atggagaatt
    14941 tcttgaataa tttttcgtgc atccgttgtg ggaaagcgaa tccttacata gtcaaacagt
    15001 aacgtcaaag gcaaatcggg attaaagctt tccagttggt caaaaatttt cctcatggtt
    15061 tttatagatg cggttacgcc accattttct atctgattta agtacggccg actaagtcca
    15121 caagccgtag ccaatttatt ttgtgaaacg ccgtattcgt tgcgtcggtc ttttaatagc
    15181 cgataatcta aattccgttg tgccaagttc tcacctccaa attcaaggtg ttaacttttt
    15241 caaatgagtt aacacctata caaattactt actatctttg atacaacaag cattcttaat
    15301 ggttgcttac ttattttttg tgtttttgta cccccctgtt agatacgggg gtgagacatc
    15361 atggtcggct aacgccgtcc atgaacaggc acagcaccac ggaggatttc tgcttcgcga
    15421 atcctccgcg gtcagcgcct aacagtcagt tgtgttggtt taggttactc atctgtaata
    15481 ccatttactt ccaactctgc tttcatttct aacaagtatt gacgaatatc tggttctttt
    15541 gcttggtccg caatccgctc aaattctgca attacttgat ttatttcaat tcgagcatcc
    15601 gtctcctcat tcattaaata tggatcgcca gcgactttga caccattctt gcgaatatca
    15661 aattttaacc gtcgcaattg ttcttctaat tctgggtctg actgaatctt tccaatcacc
    15721 caataaattt ctccaatcaa ctcatcataa accatgtaca tccaactctt ttaatcttct
    15781 ggcaatatca aaaatgaccg gaactgttac agagtttccg gcttgcttat acaactgact
    15841 atctgaattg acttgagaat ctcgatcaaa agcccaatcg ggaaagccct gaagtcgcca
    15901 acactctcta ggtgttagct tccgaatttg atactgatcc gtgacgatcg cttgttggtc
    15961 tcctgttagt aaggtatgaa cactctgttt tcccagacga cctcggcgac ttgtggaatt
    16021 aggaaaagcc agattcacac tgtcttctgg cttcacctct tgatattctg tttcattttt
    16081 tcgaatcatg atttttgtac caccttgttt cgttgacaag gtgggggcgg ttccagtaac
    16141 ttcataaact cgattacctt gttcaaactt tcccggtaat agtcctgcta cagagattcc
    16201 atcccttgct ggcagtgcga ttttgattcc ttcatcacct gttaaggttg gcgctacgcc
    16261 atctgaacca tagacttgtc cacccattcc tcgattggaa ggattgatat ttaccaaatt
    16321 tctgatgttt tgatcagatg ttgaagacgg tccaatgata ggaaatactt gttcggtaca
    16381 tgcgtctcga gaatgtccga taaggaatat ccgctcccgg ctttggggaa tgtaggcctt
    16441 tgagttaagc acctaccact ccacgtcata ccccaatcca tccagcgttc ggaggatcgt
    16501 ctcgaacgta gcccctcctt cgtgattgag caatcctcgg acgttctcaa ggaataaaag
    16561 cttaggtcgg agaagagtgg tgaaccgagc gatttcaaag aataaagtac ctcgagtatc
    16621 ggaaaatctt tgccgctttc ccgcaattga aaaagcttgg cacggaaatc cgccgcaaag
    16681 gatgtccact ggtccgaggg attgaataaa ctcatctgat acacttgtga tgtcatgcat
    16741 ttccacctcc ttacttgtat catgaattgc tttataacta cgtctagcaa atttatcaat
    16801 ttcacaaaaa ccgatacaat gatggctcgc ctgttccata cctaaacgaa agccaccaat
    16861 accggcgaat aaatctaaaa atctcataca ttacctcatt cattgcaaag aaaaaagcca
    16921 agaacatttt gctcttggct catacattat taatttgtcc tactattttt tatttaatcc
    16981 aactaatttc cgttgttttt cattggcctg atcggctaca tatttaatca ccgttttgaa
    17041 ctcttttgca gtagcatttt ccgataaatc cgcaatgctc ttattgtatt tctttacaat
    17101 tttcgttact tgttctttct cagtcatgta attgaacctc caaatctttc ctactatacc
    17161 ctagtggtaa aaataattct agattatccg tagcataatg aacttcggaa atattattcg
    17221 tataatcttt tattatgata tgaagcaata catctggatg tttactcttt acttttgcaa
    17281 tgttctctct tgatttgaaa aaagcgttaa taaatgcctt tttcggcaca tgacgtccct
    17341 ctgcaatttc acgttcttgt gtaaatttcc aagcgatttt agggtcttga taaacataga
    17401 aaatcgtcac tcgaaaatgt tttttcaacg cccgcgagat atttttttca gcacttaata
    17461 ttgcaaaagt tgcatccaga ataaaagaat agccatgatc taagacatat ctaaatgcct
    17521 gctctaccaa ccaagaagat gctttttgaa agagactaga gttttttcca ttataatcag
    17581 gaaactgggt gcgaaagtaa tccgcatcaa taatcacatg gttatcataa ttctcagcta
    17641 atccaatggc aacctctgat tttccagccc caggactccc tgccataaaa actgcgtctc
    17701 gtccaacttc gggggatttc cctttgataa tataagaaat aaacttatcc ttatttgctt
    17761 ttgcatactc taaataagaa tcattacttt ccatattaat tatccccttt cttcctacta
    17821 tataaaagta ttatacgtta cttgaagtat aaagtcagta ttgaagatca ctcaatcatt
    17881 catcttttct aatttactaa ttgaatctaa gaaatcatac ccttttggta caagtggcgt
    17941 ataaaactca ctaatcacac tgccacctgt atccacataa cctcgacctt tgatgtgctt
    18001 cataaagaag tttttttcga cctccccaaa catcatggaa tatccgagtt cactcatacg
    18061 acccaaagcc acgcgaaagt tgaattgatc gcggattccg tctcctaaat atttcgcatc
    18121 cggcctttgg caagccaaaa tcagaaagaa accagattga cgacccaaca tcacgatttg
    18181 tttgagctta ttcaaaatca ccgcactttc tttcgtcgtt aacatctcca tataagccac
    18241 gtattcatca aagattaaaa agttgggtgg aagtccaaga taggcataat tctctcctgt
    18301 tttgtagtta gacatttctt tcattgcctt gctacgagcc atcatacgtt catagaaatc
    18361 ttccacacaa gcagaaattt cttccttttg tgaataaaca tgaggcatca ccgtacctaa
    18421 atcagctaaa tctgcatttt tgggatcgag gataaacaat tctgcatctg acttcaatag
    18481 tgcttcgata atggtgagga ggaaataggt cttccctcca cccgtgccac cagcgattaa
    18541 catatggggt aaagaatcat aagcccatac ttgatttttc atcaatcgca gagtcccatt
    18601 ttctgccact acttcgtcta tcccaattcg attggcgatc atatcataca gtaaagtata
    18661 ctccacatag gagtctttga gttccttttc tactaactca caatacagtc cgctttctaa
    18721 cttcttttcc aattttaaaa gctgatcttg gtatttccca agtgaaattt gaacgcgaat
    18781 agacagtaat ccatctttca ttcgataaca gattttgggg aagtaactga tagtctctct
    18841 ggttcggcta ctattcaaat ctttgaaaaa gccttcactt tgagtttgtt tcacttcata
    18901 ccaatggttc tctagaatca tccgagctag cttttgtcga tgccacaagc ttcgccaatg
    18961 atcaagaaaa aagtgaatat aaagcaatat catagctcct gaaaaagcga gacttccaag
    19021 cagacatact aaatctttta tagaccaaac gatttcaata gtagaaagtg atagcttaga
    19081 aatatcaaat tgtctaaact cttgaaaata aaagacgctc atccaaccga tgaacagagc
    19141 gagtagccag ccaagaacaa agtgataaac caagtgctga tctcctgcgc gtatgcgatg
    19201 tcctttgtac attttgatgg tttccacctc caatcgaaaa agcccaatag gatttcatta
    19261 ggcaatttca aaaagttatg ctagtcattt actgctacga ggaggggagt tgtttcgttt
    19321 caaccaattg atttaaaaag ccttcaatag catccggcat ctccacacca atttcttcca
    19381 aaatggattc tgcctgccga cataagtttt catttacttc gacaactata aagaaagact
    19441 cttcttctcc taaccagtct agtacatcag agtcttcttc actgtcgcta ttttctccaa
    19501 gtaaaccttg atgtataatc agaaacattt tttcatcaga ggttaatgct ttaacgagtt
    19561 gttcctcagt cagctcgcct tttccatatt gatcgagaca atctaaaata tgttcaacat
    19621 taatttccat aatcttcacc tatttctttc tttcattttg gttcgtagga tttccttgat
    19681 tcggctgccg cttcaagact aaatcatccg ccttaatgaa ccaattaacg gtagttcctt
    19741 ctcgaaaaac ataattggca acggtatcca cgattggatt gaccaactct acctctgcat
    19801 cataagcaaa ctcgcgtaag ggtacattag ctgggatact aacttgaatc attcgccctt
    19861 gtgcagagga ttttaaatta taggttcgtt cttttacctc attaccaatt gttccatcct
    19921 cattttgctt ccgtacttct cggcgtaatg cggaaaaacg caacttccca aatgttaaac
    19981 ctgaatctac gacaatacct tctgttaatc tcatcttctt ttcctcctat gctttcacta
    20041 aatcatcaac atacaacacg taatcagtaa acccacgatt ctcaatctca tagcctttgg
    20101 caaccaaatg gggattcacg gcttttagcg gttggtcttg atcaaaatcc ttttcaccgg
    20161 cttccgcagc tactaccacc tcaatatcat cggcccgttg cttactggaa tacaaatgat
    20221 aactgcgacc aatcaccgta gtattccgtc catatccttc tgttataatt tctccttcac
    20281 cggcataact taactttcca aaggtttcag ccatatctgg cacgacaaat ttcaattcca
    20341 tcttcttttc ctcttttcat tgttagtttt ggatacaaaa atagcgcgac tactttttta
    20401 ggaggcggtc gcgcacgttg gttcttagga tttacttgta tcatctcatc accttagttc
    20461 cggcgattcc agaaataata gcccgccgtc gcaaaaatca aagtaaaacc aatgaacagt
    20521 aaaacattgg aatttttctc cccggtttga gggaaggtct tcactgtggg actagattct
    20581 tgagagccac ttggaacagt tggtgtttcc ggttgtttcg gcgtacttag tatggttgga
    20641 acttcttttg gtgttaaggt ttgagatttt tctttcaaat cttcattgtg ttttccattg
    20701 atattcccat ctttatcgta attgatttcc gcaaaagtaa acttggttcc ttctggatat
    20761 tttccggtat ctactttttt cgcaagcaca gttttggtga attctttatc atttacttca
    20821 taatcaatct tgccagattt ccaaatttct ttgtttgtac catccggtaa taaagcatac
    20881 agaattgttt cgaaagcttc ttttgagcca tccagtacat catgagtaat cgacacatca
    20941 tcaaacatat ccaccacatc accatgagta aaggtctgag aaccgttttc taggtgggcc
    21001 tttgtttgga tagaaacatg gtgttccact gtacaactga cagtttgggc ttggttattc
    21061 aagctaacat ccttcgctac tggcttatcg cctgcttcaa aagcttcctt attttcatac
    21121 acatagttga ataagacaat agttttattt aaaacttgtt cggcggttaa tttatgctga
    21181 attttccaag ttcctgtttt ctctttgttc gccaacgttg cgcttgtttc tgcaatcacc
    21241 actggtttgg ctttttcatc tttttcttgg gcagcttttg tcgcatccac atcaatggct
    21301 tgcgctacca cataccagtc ttctttgatt tcatgtagat tgtaactcaa tttatcaact
    21361 agctcggtag aatccagaga agttaattct ttgtttccgt ctttccaagt cgcaagagaa
    21421 gttaaactat cttcttcctc aggcaaatca tagagcatta aacggttcaa actaacagaa
    21481 aatgctttgt tcgtcagctt ctcgtaagga acggtcaccg ttttaattgg ttgtttttgg
    21541 tcttgttcgg taatggtaaa gacatactca ctcttcacaa agtcttcttt gttttcctta
    21601 aaagtagaac ggatttctaa tggcgtaatc ttttgaaaac cttctggggc ttctacttct
    21661 tcaagtaaat aatccccata aggaagattt tcaaacttgc cataaccatc aaaccctaat
    21721 tgctcgttat aagctgtggt cgcttcatct tcggcacctg tgatttcatt ggtcccttcc
    21781 aatggcgaca ctttaaaggt cagatcattg aagccagttt cggtagtgcc cgcagctgat
    21841 ccagcaaatt taaagaaatc aaagccaaag cgaatcactt gttcttttgc cgtaacattt
    21901 cgggtaatca cggcattttt ttcgctatca tccacctttt tgatggatac aggatacttt
    21961 gtttcatcaa ggctatatcc ttcgggcgct tttgtttctt gccaataata ctcgttgata
    22021 gctaaatgtt taacggcaac ttggttcttt tcgtctaaag tcaaagtgac cgtttcatca
    22081 gaacctttcg ttcccttcgc taactctggt ttaaaagcct cactccattt aacagctttt
    22141 ccatccttag cagtaaacaa ggtgtattca gcaccctcaa agactgcctt accttgtgct
    22201 ttatccccag tatctttgtc ttctttcgtc aaagtggttt cgccagtgac ttcttgattt
    22261 tgccctttta cgttactggt aacaggggct acggtttgat tggcatactt caattcgact
    22321 tttacaggtt tgaacgtatt cacgaaccca tgactagctt tagtttcggt cacataatag
    22381 gttcccaatt ccaacgcatt agcaatctct tttggtgttt ccgcatgacc gttttcatcc
    22441 gtggtcattt cttgaacgat ttcaccggtt ggactgtctt tgcgaatggc aaacgtatta
    22501 ccagctaagg aataattgtc attccaaaga tcactccctg tttcaactcc gcttttatcc
    22561 agaatgattt gccctttttc ccgtgcgttt tttgaagtca cgtaaatggt ttcgcctgct
    22621 ttaatggtgg tagtcatggg ggtggtatca atcgtataag gcgccggtac tgatttttcg
    22681 gtaatggtta cttttgtacc atgaggaatc tcatccaatg tagcaatgcc ttctttgtca
    22741 gtagtcacgt cttttgcagg taaagttttt ccaaagtcta aatggaaaac cgttcctggt
    22801 acaacatcac ccgattcttt atcgactttt ttgatcttta aagaaccctc cgtttccaca
    22861 tcaattttga ttgtgtaggt attaggttta tcaatcgccc cggccattaa agtctgttgg
    22921 ccagcctttt tataggcgac aggtgttccg gtaccagcag atttcttaag cgtcagcaca
    22981 ccactttctt ttgaattggc atttggggtg attatcaact gattaccatt cacacgataa
    23041 tcaatgtttg cggcattttc cacaacctca tcgaactctg acaaattcag attattcgta
    23101 tccgtcacag tagtcgattg ccctaatact gttttagccg tactattatg gaaggttggt
    23161 ttcttctgat aatcgtcaat ggcttgattt atttttgcct caatggctgc gatatttacg
    23221 gcactaccat ctgatcgttt tattgagtga agggtatacc cattaacttc ttgccagatc
    23281 atcttctgtg ccacaatatg agtatctgca tctgtaccta cttttttcca taaaacggaa
    23341 actaatttgg ctttatcgga catatctggt aacgggtttt tctcatagcc aggagtgaac
    23401 tcattgtaaa tcgggactcc tggttcaata cagaaaatat cttgtttaaa gccgttgtac
    23461 actgcataat gcggaacagt gacttctgac attggacgac catcactaaa agttccttca
    23521 acagcatatt gatgtgctaa atctagttct acggttaccg tttgtggatg ggtaatttca
    23581 tcagcaaaag taatcgtagg agcaattgct ccaacaattg cttgaacagc caaactcaaa
    23641 atagttaata cacataattt ctttttttgt tttttataca tgaaatgttc tcctttcaaa
    23701 ttaagcacaa aaaaataaga tgctcgcttg aacatctttg tttataaagt ctttttaaaa
    23761 tttttattga ctgaatagaa tggatttgag aatgaatgag
//
LOCUS       DAYYGS010000041        23700 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000041 DAYYGS010000000
VERSION     DAYYGS010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.041"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            35..532
                     /locus_tag="ACHA44_001515"
     CDS             35..532
                     /locus_tag="ACHA44_001515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904794.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(515..1252)
                     /locus_tag="ACHA44_001516"
     CDS             complement(515..1252)
                     /locus_tag="ACHA44_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFM8904795.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
     gene            1694..3334
                     /locus_tag="ACHA44_001517"
     CDS             1694..3334
                     /locus_tag="ACHA44_001517"
                     /EC_number="4.1.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043883 - malolactic enzyme activity
                     [Evidence IEA];
                     GO_process: GO:0043464 - malolactic fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malolactic enzyme"
                     /protein_id="HFM8904796.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3488..4810
                     /locus_tag="ACHA44_001518"
     CDS             3488..4810
                     /locus_tag="ACHA44_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HFM8904797.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4878..5312)
                     /locus_tag="ACHA44_001519"
     CDS             complement(4878..5312)
                     /locus_tag="ACHA44_001519"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8904798.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(5377..6213)
                     /locus_tag="ACHA44_001520"
     CDS             complement(5377..6213)
                     /locus_tag="ACHA44_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFM8904799.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6955..7581)
                     /locus_tag="ACHA44_001521"
     CDS             complement(6955..7581)
                     /locus_tag="ACHA44_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904800.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7876..8211)
                     /locus_tag="ACHA44_001522"
     CDS             complement(7876..8211)
                     /locus_tag="ACHA44_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFM8904801.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(8198..8482)
                     /locus_tag="ACHA44_001523"
     CDS             complement(8198..8482)
                     /locus_tag="ACHA44_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFM8904802.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(8538..10061)
                     /locus_tag="ACHA44_001524"
     CDS             complement(8538..10061)
                     /locus_tag="ACHA44_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFM8904803.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(10054..11229)
                     /locus_tag="ACHA44_001525"
     CDS             complement(10054..11229)
                     /locus_tag="ACHA44_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8904804.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(11233..11418)
                     /locus_tag="ACHA44_001526"
     CDS             complement(11233..11418)
                     /locus_tag="ACHA44_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904805.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(11384..13078)
                     /locus_tag="ACHA44_001527"
     CDS             complement(11384..13078)
                     /locus_tag="ACHA44_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFM8904806.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(13075..13548)
                     /locus_tag="ACHA44_001528"
     CDS             complement(13075..13548)
                     /locus_tag="ACHA44_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFM8904807.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(13617..13772)
                     /locus_tag="ACHA44_001529"
     CDS             complement(13617..13772)
                     /locus_tag="ACHA44_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HFM8904808.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13906..14286)
                     /locus_tag="ACHA44_001530"
     CDS             complement(13906..14286)
                     /locus_tag="ACHA44_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8904809.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(14290..14505)
                     /locus_tag="ACHA44_001531"
     CDS             complement(14290..14505)
                     /locus_tag="ACHA44_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904810.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(14508..14915)
                     /locus_tag="ACHA44_001532"
     CDS             complement(14508..14915)
                     /locus_tag="ACHA44_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HFM8904811.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(15181..16656)
                     /locus_tag="ACHA44_001533"
     CDS             complement(15181..16656)
                     /locus_tag="ACHA44_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFM8904812.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(16646..17494)
                     /locus_tag="ACHA44_001534"
     CDS             complement(16646..17494)
                     /locus_tag="ACHA44_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HFM8904813.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(17531..17893)
                     /locus_tag="ACHA44_001535"
     CDS             complement(17531..17893)
                     /locus_tag="ACHA44_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904814.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17894..18052)
                     /locus_tag="ACHA44_001536"
     CDS             complement(17894..18052)
                     /locus_tag="ACHA44_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904815.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(18193..18817)
                     /locus_tag="ACHA44_001537"
                     /pseudo
     CDS             complement(18193..18817)
                     /locus_tag="ACHA44_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18814..19125)
                     /locus_tag="ACHA44_001538"
     CDS             complement(18814..19125)
                     /locus_tag="ACHA44_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904816.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(19169..19462)
                     /locus_tag="ACHA44_001539"
     CDS             complement(19169..19462)
                     /locus_tag="ACHA44_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904817.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            19656..20303
                     /locus_tag="ACHA44_001540"
     CDS             19656..20303
                     /locus_tag="ACHA44_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8904818.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            20364..21509
                     /locus_tag="ACHA44_001541"
     CDS             20364..21509
                     /locus_tag="ACHA44_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8904819.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21948..22766)
                     /locus_tag="ACHA44_001542"
     CDS             complement(21948..22766)
                     /locus_tag="ACHA44_001542"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904820.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(22866..>23700)
                     /locus_tag="ACHA44_001543"
     CDS             complement(22866..>23700)
                     /locus_tag="ACHA44_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904821.1"
                     /translation="LFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYS
                     LWSEVFSKYMDVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEK
                     AFQKILAGDIAGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKK
                     SMESIGQTLSVQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLS
                     ALSAQRAIMQNFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKS
                     E"
BASE COUNT         7017 a         4743 c         3879 g         8061 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt ggactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt aattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaatag tcctcatttc ttgggagttt tgagactact atatgcgttt
     1321 ttttgttgat ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca
     1381 cgggtttcta cttatgtatt tttatcacct gacataggtg actcctatca acgctatcat
     1441 gtgctttttt cctcagaaat cctatttatc cattccatct atcccttatt cttctacttt
     1501 tctttctcta gcatataaga gatacgcata gtattcaagc aaggattgtc gatataaaaa
     1561 gaacaaatac tgctattttg atttcttttg gctatattac atataaataa aatttatcat
     1621 acctgctctt tttgactata tcatcatctg acttctatac ttcgtttgta ttaaaactta
     1681 ggaggaatca ttcatgctta cagggttaaa cttattaaat aatccttttc taaataaagg
     1741 aacagctttt acaaaagaag aacgggcaaa atatggaatc actggtatgc ttccaagtac
     1801 tgtccaaaca ctggaacaac aatctgttca agcttatggt caatacttaa gcaaacaaac
     1861 agatttggaa aaacgtatct tcttaatgaa tctattcaat acgaatcgga cattgtttta
     1921 taaattgatg ggcgaacatt tagttgaatt catgcctgtc gtttatgatc ctgtcgttgc
     1981 ggattcaatc gaacaataca acgaaatttt cttgaaaccg caagatgctg catttctttc
     2041 aatcgacgaa ccagaaaaca tcaaagcaag tttgaagaac gcggcagatg gacgagacat
     2101 tcgtttgatt gttgtgactg atgcagaagg aatcttaggt atgggcgact ggggtgtgaa
     2161 cggtgttgac atcgctatcg gcaaattgat ggtctatact gctgctgctg gtattaatcc
     2221 agcacaagtt ttgcctgtat cgatcgacgc tggtactaat aatgaagaat tattgaataa
     2281 tcccttatac ttaggcaatc gtcatgctcg tgtagaaggc gaaacttact atgaattcat
     2341 cgatcaattc gttcaatcag ctaccgaatt attcccagaa ttgttattgc attgggaaga
     2401 ttttggacga ggaaatgctg cagcgatctt agaaaaatac gaagatcaaa tcactacttt
     2461 caacgacgat atccaaggta caggaattgt tgtcctagca ggtgtattag gcggattgaa
     2521 tatatccggt gaacaattga aagatcaaat aattttgaca tttggtgctg gtactgctgg
     2581 tgtgggtatc gcaaacatcc ttttagatga gatgatccgt caaggtgtac ctgaagaaga
     2641 agcacgccaa catttctatc aagtagataa acaaggttta ttgtttgaag atacagaagg
     2701 attgactcct ggtcaaatcc cattcgctcg caaacgctca gatttcacta atgctgatga
     2761 attgacaaac ttagaagcag ttgtaaaagc gatccatccg acaatcatga ttggtacttc
     2821 tacccaacct ggagcattca ctgaagaaat cgtgaaagaa atggctgctc atacaccacg
     2881 tccgatcatc atgcctttgt ctaatccaac aaaattggca gaagcaaaag caaaagattt
     2941 aatagaatgg acggacggaa aagcattagt tggaacaggt atccctgctg ctgatgttga
     3001 atataatggt gtaacttacc aaatcggtca agcaaataat gctttgatgt acccaggttt
     3061 aggacttggt ttgattgctt caacagctac acgagtaaat gctgaaatca tttctcaagc
     3121 tagtcgagca ttaggcggaa tcgttgatgt aacaaaacca ggagcagcta tcttgcctcc
     3181 agtagcaaaa atcactgaat tttctcaaac gattgctgaa actgtcgcaa aatcagttgt
     3241 cgcacaaaac ttgaatcgtg aagaaatcac ggatatcaaa gaagcagttg aatctgctaa
     3301 atgggttcct gaatataaat ctttagaaga ttaagatcgt tatagtaaaa gaacatacag
     3361 aaactttgta tgacgtctaa aaagaaaaaa gcgactgcgg caaaacagcg gccagccaac
     3421 tcctgctctt ttgtcgcttt tctttggctt ttttcatatg atacagaata agaaaggatc
     3481 tgtaaaaatg gcacaaaaaa caacaactgc aactgcagtt gaggaaaaga aaaaagaagg
     3541 tttttggtcc acaaaaatca ccggcgtaag tttgccactt tatttgatca tggtccttgt
     3601 cctgatcatt gcaatggctt tagggaaatt accgacgaat atgattggac caatcgcagt
     3661 tttggttatt ttcggtaatt tatttcattt tatcgggaca aaaatcccta tcgtcaaaag
     3721 ttatttgggt ggtggttccg tttttgcgat cttcgcttct gctgcgattg ctacatttgg
     3781 gatcttacct gaatatgtag tgaaatccac agaaaacttt gtcagcaata tgggcttttg
     3841 ggacttttat attgctgcct tgattactgg tagcatcctt ggtatgaacc gtaatctatt
     3901 gatgaaagct tctgttcgtt ttattcccgt tgcacttatt tcaatggtcg tctccttttt
     3961 tgccgtagga ctcgtgggca tgcttatcgg aaatggtttt gcaaattccg ttttatacat
     4021 ctcattacct gcgatggctg gtggtgttgg agccggagcc gttcctttat caacgattta
     4081 tgcaaatgtt ttaggtacgg atgcttcttc tatcatttcc cgcctgattc ctgcgacagc
     4141 aatgacaaat gtcttagcta tcatcggtgc agcattagtt gctcgtgctg gacaatcaat
     4201 gccaaaattc aacggcaatg gtaaattaat ggaaaaaggc gatcttggcg atggaaaacc
     4261 tcgaactgtg aaaccttcca ttccacagtt aggtgttggt ttgatggtat cattgacttt
     4321 cttcatctta ggacaaattg gtaactattt tgttcctaaa gtccatgctt acgcatttat
     4381 gattattatc gttgtcatct gtaaaatcgc caacctatta ccagagtatt atgaagatgc
     4441 agctatcatg ttcaataact tgattgtcaa aaacttgaca gcagctgttc ttgccggtat
     4501 cggtattgcg ttgctgaatc tgaatgtatt agcttctgct ttgacatggc aatttgttgt
     4561 cttatgtctg acaagtgtta tcgcgatttc agttgtttca ggatttgtcg gtcgtttgtt
     4621 tggtttatat ccaattgaat caactattac tgctggctta tgcaacaact cgatgggcgg
     4681 aacaggaaat gtagccgtct tatctgcagc taatcggatg gaacttattg cttttgctca
     4741 aatgggcaat cgacttggtg gtgccatcgt tttgatcatc tctggtttct tgatgcaatt
     4801 actttcttag tcaaaaaaac aaaaatacct gatcatcaag tatcggcttg gtgatcgggt
     4861 atttttttga taaagattta caggattttc aaaaatcttt tttgcatttt atcggaatga
     4921 taaccgatgt gttcataaaa ttggtgtgct tctactctgt attctgcaga attaagcctt
     4981 attgtcgtta ttcctcgttt tttcgcttct tcttctacgc cagtcatcaa agcctttccg
     5041 atgcctcttt tttcagcata tttagacaca gcaagtgcca taatattgaa cattggatcg
     5101 ctaaaaatcg attcatagga ctccgcttgt acatagccag ctgctttttt agaagtagga
     5161 tcttcataaa ccaagaaaaa atttttctct ggctccttca gcagtttctt catttgtctc
     5221 accgtaacgg ttaatggtat atcataccct aattgatccg cattgatttc acgtagtgct
     5281 tctgcatctt ctaaacgcat ttcccgtaac atccaaatca ccagccaatt tctgattttt
     5341 taaaatgaat ttttttatca tagtatactc tttttttcat ttagaacaat atgtttcagt
     5401 gattttgact tgcgttagtc tagattctcc taatgtttgt aagatgacaa aggcattgta
     5461 acaattgacc aaaagaacca cgaggacaat tacttgaaaa ataaaggtca ctttttttgg
     5521 agacaaaacc ctgctaaatc ttgctcccca caaacctcca ataacagcag cggggataac
     5581 ataaaacaac atagataaat caaagcgcat aaagctactt gttaaagcaa tcgttaccaa
     5641 ttttgccagt tgagaaaaga aaatcgtaga tagagaatat agtgttgctt cttttattgg
     5701 caatgcaaac atcagcatca acaaagaaac attgactggt ccaccaccaa tacctaaaaa
     5761 actagctaaa aagcccagaa cccccccaca tataaggtac caagtccatg acgtcaaatg
     5821 aaattttggc tgatgatgct tggtgtagaa gaacgcaaaa accaaggtca ataccgtcaa
     5881 aaaaatttgg atcagctgca catgcttctc gttttcaaat aactgtaaaa aaacttcgaa
     5941 tacaatgttg ccgactactc ctccaacaac agctccacta gatacccaac cgacaatctg
     6001 ccaattcagt gatttcccgc ttgatacttg tcgcacagtc gaaacaatgg acatcgtgaa
     6061 taccgcaact gttgagtaaa aagaaatccc tgcaacagaa tgggcgccga taagatcaag
     6121 aatcggtttg atcaacacgc ctccacccat cccggaaata gcgccaactg tgttcgccag
     6181 aacgatcacc acaaaataaa tcagaccaat catcttgtat ttcctttctc ttgtttatat
     6241 cttttagcga ttgttactct tttcttttat tatacagtaa aagaaacaaa cgaggcttcg
     6301 aaagcttttc tactttttaa ggaaatttag tgctaaacga aaagaggttg tgaaaaagct
     6361 gtcctgcatc aagtaataag aacagccaaa caaaaatagc tcctcataat tttcgtttgg
     6421 ttgaacttat taccgcagat gcaagctttt gaacaccgtt taagaaagag gtcgtgagcc
     6481 tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga
     6541 atattgtgac ttgtgaccga ggagttggca ggagaacacc gtttaaggaa gagactgtga
     6601 agtcaacgtt ttgacctgat tattagagaa acaaatggca aaatagttct tcatatttcg
     6661 ccgtttttcg agcgaatgcc gaggggcagc tgattgaaca tcgtttattt ggaaaaaggg
     6721 attgtgacaa gacgtttgtc acaatccctt tttttatatc ttattaattc attcttttgt
     6781 tctattcttc ttcttccgcc atgaatgaga aacccatatc aaagaactat aaaggttatc
     6841 aaaacagcgt ttatgactaa atgaattaga gttatctatc actatttttc aagcgattta
     6901 aaccaaatgg catacaatat ggcatacaaa aacttcaata agccgtgggg gtttttaaat
     6961 ttcttcttgt tctttaaaat caatgagtgg agctacataa tttgaatcca ctttttcaaa
     7021 cgtcactttt tgcatcgacg aatgttttct ttcccaagat aacgtaaata gatttgactc
     7081 tggacttata aagtctactt caccttctag tattactttc ttatgcaaat attcctctaa
     7141 taaatataga cttgctaaag catacaacac attttccaaa tttgcttcct tataatttga
     7201 agaacgacca tgttttactc tgttatatat tttccaccaa tctggtgtat cttcaggcca
     7261 cccatctcca aaaggtttta gtgaagtcaa tgttaaagaa ttcataaccc taacaccatt
     7321 atccaaacac attaattcgc tagatttaat aacagcttta aaatcagtta tgcttttatt
     7381 gctactgcta tcaaatccgc aaagttcttt gaaaataatc tcacactccg aaccaatcgc
     7441 tagtagtagt ttttgatatt caatagagaa tgttttgaaa ttattctcat cgagcatcac
     7501 aaaatctgtg gtattaataa actcattttc taaatgtaga taatacttcc aataaaaatt
     7561 tgtaaactct gctttgtcca ttaaatcacc tcttttgaat tataacaaaa aaaacacgtc
     7621 cacaacagac gtgttctttg atttctacac aatcagcttg ccaaaaccat ccaatcagtc
     7681 ctagcatgcc tgtattaaaa ttgaacgttc atcatctatc atctttctta gtccagcatg
     7741 tttgcaacca cacctacaat tttcagtcca acaatagatt tcgactagct aagtcttgca
     7801 aaattcaggc agctaaccct tcatttacta ccttaattat actaaatacc cacaacgttt
     7861 acaagtgtaa tcattctaag aaatctgttt agctatgatc gttgtataat ctttaaacgc
     7921 cgttccttca ttgattgaaa taattttata actcttgttt ttccaagcta ctttcatatc
     7981 gtttgttagt tcataatctt gatcgtatcg aataataaac gtcaacgttc cttcaagaat
     8041 ggtacctaca ctagcaagca cgtcttttag catttgtgaa cgcacattag cccaacattc
     8101 aaatacagtt tcttcactat ccaccatctg cccatcttca tcttttaccc gttttgtctt
     8161 tttaaacttg actctctcag tcaattcatt tacattctta atgattggca taataagcac
     8221 ctctcatttg ttgtactaat gcttgtaccg ttactggtat ccgttcgctg ctggcttctt
     8281 gacggttcaa ataccaatgt tgagtaagta atgacaccgc caaatcaaat tgctgataat
     8341 tatccatatc tttatccgtt gcgctactat caatagcact ttttatatat tcgcctgctg
     8401 tatcaatcaa ttgttgaata aagtcatcat caatagtgtg atcgatccgc atactttttt
     8461 ttatgctatc caaaattacc attttatctc tcctaatgaa aaaaaggcag tgggataaac
     8521 cgccgcccaa tatttttcta tacttttggt gttacaactg gcgtaaactc aatatacact
     8581 gaagcatttt gatcaatttt cttataatcg ttgcgaacga tcactgcaag tccttgtgag
     8641 tagtaatcga atttttccca ttgagtcgtt acttgatttc tacgagcaac aaagatagat
     8701 tgtgcaatat cccccataat catagggaat gttccagctt taggattggc aaacaatgta
     8761 tctgcaatca atactactgg cataccgaat aatgatttac cactaggtgc tgtcacatcg
     8821 ggttgtaaaa tgtaacgtcc atctgaatct ttcaatgtat ctaggtggta atatccgctt
     8881 tggtttagaa ttaccatttt atttaatgct gggtctaatg ccacattgta tagttgtttc
     8941 aaatcgtcca acgtagcagc cgttttctta gtgaatgttt ttaataaatc catgatatgc
     9001 ttattgtcag tgttttctac caatttcgct aactgatctt tgacctcttg tacaatattc
     9061 actgctgaat cttccacaac ctcatttgat aaggcaatct taccagcacg agtttctact
     9121 ttataatcaa ctgaagtaaa catttcagcg tcaatatcac caatttcagc aagttcagct
     9181 tttgttgcta acactgcttg ttggttagtt gctactggat atttaccttg tccatttgat
     9241 actgttttta ctgttgcata ttgagctaag ttataatttg aacgtttcaa atcaaagact
     9301 tcaccgatta cttcttcggg aacaactgcc gctgcattta cagtagtgac tccatcacgc
     9361 acttcgcctt gtgaacggat atattcttca tacccacgca attctgtttt ttcgttatcg
     9421 attaaagttt tttccatgtt attactctcc ttttgtgctt tagaagtgga ttctaagcta
     9481 ttgtttgttt gttttgcttg gttgttactc ataaacgatt cgtaagaacg cttatttact
     9541 tggacatttg atgaatcata agcgggaacg gtcacgacac taatttcatt aagtgctttg
     9601 attttatcta ttgatcgttc aattgtgcca tcttcttttt tgtcgaatga atctaatcca
     9661 agcacgaacc caaaggacat agaatcaaca accccttttg agatattctc atacacatca
     9721 tttgaatagg tcgtgtcggt tagctctgct tcaaaatgta atcctgtatc atcgacattc
     9781 aatttcaacg tacctgcttt gacactagct agtggctttg aataatcgtg tccatataat
     9841 aagaacacat ttgataaatc cacctctttt aatgcttcgg gtgtaatcac ttctacaaag
     9901 ccgcctaaat cttttgacgg ttgcccgaat ttcaacgcat agcctgaaat agttttgccg
     9961 tctgttggtt gtttttcatt ttctttctct actttgggag aatcggctgt tagctcagct
    10021 tcttctgtca gccgtttttc cttttcttct tcattattca actggtgtaa ctccttcctg
    10081 ttcttttaat tgatatttct ccaacgtatc taaatacgta aagttcaaac ttgctagtaa
    10141 acgatcgcca ccatccatgg ggggtagccc cattttcgac cgcccctcat taatcgtgag
    10201 aagtgaccct ttgacctgat ccaatacatt ttttactttt gtttcaggat cagtctctag
    10261 taactgatcg gaattaaagc ggatattggt ttcaagtttc gtatctaatt cactcacaaa
    10321 cacattaaag taatgaatca gcgtactttg gatatactgg agattacttt gaacggtgct
    10381 agaatgttca ttttcaacgc ctaaacgctc aatcggtaca ccaaacgcct tggcaatttg
    10441 ttttgtgttc caatcattgg aattgataag cttcaaaaca tcggtgttta tttctaatgt
    10501 tttgtagtcc attgtttcat caagaatgat cgttctaaga gcattatctc cactacttga
    10561 accgttggct tcttcaaact tttcacgaat tgcgttcttt gctgatccgt ctaaatctga
    10621 cttatgaacc ttcaaaatac ctgacccact gacaccacga gtaaagaaat tgtgcaatgt
    10681 gcggttgcca gcttgttgaa ttttcagttc atcacgtaac gcataaagtg gtggtaatcc
    10741 tgttaaacca tcttgggtga aatatttgaa gtgcaaaata tcgctagact tcacacgtct
    10801 ttttttatca ccaatctgat aagacaacgt accattatct aattgagtaa ccgttacact
    10861 tgagttaggt aataagtgaa tttcttctac cttttcaccg ttgcgtttaa tctctgcaaa
    10921 gctattaccg tttagcaaca tattgaccgc caaagcaaac tttaaatgcc aaccatccat
    10981 ttctgaatta ggtttctcgt ttagtaactt caccagttca ttatcagctt gtggcatatt
    11041 gtttttgacc aattgaatcg gacttgaagc aatatcacta gcaataattc gaacggctgc
    11101 aaacacatca ctatttctta atgcacgaac gcttgtaaag ctggtggtgt aatcatccga
    11161 ttggattgat accacatgtt gtaggaaagg atctcccgtt gtattttgtc tcgctttaaa
    11221 aaatgccatc ttttaacctc ccttctgttc tgtatttagc atgaccgcta ccccaataag
    11281 cactatgccc agcgctaaca agccatagta gacatttgtt agtaaggtga tagcaaccaa
    11341 cacacacacc aatccaagaa gtaaaagaac ggtatgaatg taattagaaa gaaaattctt
    11401 cacttgtata aaattcattg tctgctttcg ctccttccgt tgtggtgaag taatccattg
    11461 caaacacata ggcattgatt aatgcagcta ttggatcaat tttgttactg ttctttgatt
    11521 tattaatctg aatgccgttg ttatccgttt ttaaaatagc gttgttcact gcatgggtga
    11581 gaatcgtatt tttattgtga ataacgttgc cttcataaac ttgttctcta aaagttcggg
    11641 tcggaacgtt tagagtaatc gttccttgtc ttacttctaa cattgggtag ttggcttttt
    11701 cagctttact gataagcgaa ttagcgttat acgggtcata acaaatcccc atacattcca
    11761 aatcattttc ttgaatcaaa tcttgaataa actgaaatac ttggtcatag tccacgattc
    11821 cactttctaa ttgagtaatg gaacactcac cagctttttc tagttctcga taaggcaaac
    11881 cgtcgcgctt ttctttgtct tgaagcccat atttggtggc tacaaagcta tgtgaatcac
    11941 aataaagttc gccgttatct agcggtacga tccatgaaac gctagttaaa tcatctgttt
    12001 tagataaatc cactccgata taaacgggct tgcctgtaat atcttgtggt tctacttcgg
    12061 ttgcctgcca gtcttcagca atcatatagc tgtcctcact cgcttgtctc cataaattga
    12121 agtttttcac caatacagaa ttaaggttat tttgtttcaa agcaaggttc acatcatctt
    12181 gaatggttgg aatcattact tttttgattt cttcactttc aaaaatcgga ttggctttga
    12241 tccatttctc ttgatcgtga atttcttcgg gatcgtccaa ttcccatatc gctataaagt
    12301 acctgtcggc ttgttcttcg ccttttagca cacgatctaa caataagtat tcctcgtaca
    12361 taggaacgtt taaatcaagc ccagcggtac taataacaca caataagccg ttcttttgtt
    12421 gggtcattcc tgacttaatg acgttgtacg ttttacgagt ttttgcttcg tgccactcgt
    12481 caagaattgc ggttgttggt gcaaacccat ctagcgtgct ggtttcacta gccagtgcca
    12541 tagcgaacga attagagggc aagtgctgaa tcttagagtt cataattttc agttgtggtc
    12601 ttaaaaactt actagacttg accacgtttc taagtgaatt agacagcata tcatagccca
    12661 atttagcttg tttcaaagcg ttacttacaa acaatacttg ccttgcttct gctggttctt
    12721 gttccataat caacgcattg gcagccatgc cacttgcgat atacgttttc ccattcttac
    12781 ggctcatact ggtaaacgct cgattgaatc gcctgtaatt cccttcttta gtacgccagc
    12841 cataaagact gcctagaatg aatttttgaa atcctaacat cttgattgct ttaccgtccg
    12901 tagatggtag caactcaatg aatttaatcg ctttggtggc ttgttcttca tcgaacacat
    12961 aagggaagct atctgactta gaacgctcca aatcgcttat atgacgctca cacgcttgga
    13021 taattttctt gggtgcaatc agcttacctg ataggacttt ctcaatgtaa tcattcatga
    13081 attcatcaac tctttgaacg ggtcttcggg ttcatcattt ttaaccttgt tcaaagctag
    13141 ttttgatcgt gattctaagg tcatacctag tgaggtggct tgttttttca aatcactcat
    13201 agcttgtgat tgaacccgaa ccgctggatt ggctttcttt actccgtttt cgcatgtgat
    13261 aaacgtacct gattttctta tctcggcttc ggctgttttc attcgagcaa aggctaaaca
    13321 ataggctacc aatgaaccat aatctgcttc actcaatgga taatcttttt ttaatgttgg
    13381 tacgatacga ttccattcac ttctagcgct cgctgccatc cagtcagggg gcgtgatatt
    13441 tattaatggt tgctgcttga acaattcttc tctagcgtcc acacggattg ctttttcttc
    13501 acttgatata ttgccttttg tatcgtgtaa tattttaggg tgtcccatgt tgtcctcctt
    13561 tcagacaaaa gaaaaaggaa cgtaagcaat taagctcaca ttccttcgtt tttcgatcag
    13621 aatttttctt tatctaattt atctatataa atgatttttc cgttctgcat ttgaatggtc
    13681 acttgaccaa aatgaggtaa ctcaatggtg cgctgtttcc ctgttttatc agtaataaca
    13741 attgtatttt tcaataaatc tcctactttc attatatact ttttcacttg tgattacaag
    13801 tgtaatcgtt atttgtaatt acccttatgc cttttcacaa cggttatttg taccaaaatg
    13861 agtaggcatc gattgtcagt aagtttcaca ctatgccccc ttaactcaaa gggtactctt
    13921 ttctcgtctt tgtctttcat tggctgtctt ggaattatgg tgtgcatggc ataaagaacg
    13981 aaaattagtt tcatctaacc gtctattcca atcatctttt agttcaattt tgtgatctac
    14041 aacatcagct ttccttatca agccttcttc taggcaatcc tcacatactg gatgatttaa
    14101 ccgatacaag tatgaagcct ttcgccatgt tctactttga tagaattgaa aatatttacc
    14161 ttcgctttgc tttctttctt cacgcttaat tgccgtttgc tttgctttat gcttcccaca
    14221 atacttctgc ctataatcaa tcaatgtttt acatccagca tgattacatt gttttttaac
    14281 tgccatagat taccaaccaa agcgatctcg ttcgtcagaa tcatcttgtt cccattttga
    14341 tttgtattca atataaggcg aaactatctt caagcacgtt ccgtctttaa aattaacttt
    14401 agtgatttta tcttcctcaa aactaataaa gtctacatct tcttgagtgt ttgaatactc
    14461 aatgtgtttc tcaccatcga agtatctaat actttccata tgcataatta ttctacctcc
    14521 aataaattag ttatcaattc atcatctgtt agtagcaaag ccaagaactt atcatggatg
    14581 tttaaaaaca aattatcctc attcgatgta agctctcgtt gtctcgctcg atcatatagg
    14641 ttaagcagac aattaaattc atccattagc tcacctgtat catcatagtt gccactatct
    14701 accaatgatc ccactactag cgattgacac cacgctactg actcctgcag ccaatcaggt
    14761 tcttcttcca ttatctttaa tacttctgga tcaaatgtgg aataatcttc tgttaccaat
    14821 cccacactaa atgccagcgt gttcattaga aaccgatcca tttcatctgt atattgaaag
    14881 ttcggtgctt tgtttgttga aataatattg agcatttact ttcctccttg attcgtaaca
    14941 cgtggtaaca cctacgtaac aggtacccgt taccactcaa tcccaacggg gacaagggtt
    15001 ctagcgtttt ggtaacaggt aacgggtaaa atccagtaac attattcccc agcatattag
    15061 ctaactattt caaaagtttt aaggaaataa ggtgttaccc cgttaccaaa ttgctgtatc
    15121 ccttggggca gtaagggtaa actagtaacg ggctaggtgt tactgacccg ttactacccg
    15181 ttactttttg tactttccag ctcgattttt attttcatag gcatttgact tattgtaaaa
    15241 tcctcgtcgt ttatttcttt ttggcagttg cttccgttcc caaccaggaa tactgctaat
    15301 aatcaaggca atctttttag tttctgcatc tgatcgacca ttcaataaat cctttgcttg
    15361 tttatcaaaa acagcttcca aaatatcagc ggtgagtacg ctatcaacta aataaaactc
    15421 ttgttgcata gtagctccat tatagaaatg ctgtaaattt ttctcattac atttactgtc
    15481 aggatagttc tgataataag aacgtctcgc ccacgctgga gcttcttccc atttatacgg
    15541 gatttccatt tccaaatatt tagtgattgc ttctttaatt ggattctcca tcttggcatc
    15601 ctcttgatag tcttttgcaa tttctaagac ttctttatct tcatcgggac taaaatagat
    15661 tcgttgtcct ttatcaaaaa gcacctttgc ttcagctaac acttgaagaa aatattcatc
    15721 ctttgtttta aacggattca tctttggctt attcttacaa ggcaaaggat aaaaacggcg
    15781 gtttcctgta tcatctttca aatactgccc atcattcgac gttccaataa atacacattg
    15841 ccttgcccaa gctatgacat tccgaccata aggaggtcta attttatcga tcttggcact
    15901 gatgaagttt tttatctgtt caattttagt tttgttcatt gaagataatt ctccaagttc
    15961 aataattaca ttgccttgaa gctgcatata atcatctttc ttttcaccta gcgaatctaa
    16021 tgaatcggtg aaaaaatcat ccgtgtaaag taatgctgga acggtactct taccaatccc
    16081 ttgcggtcca tccaaaactg gaacgatctc aaactttatt cctggtttat agatacgtgc
    16141 gactgatccg actatccaac gttttgttac ttctcgtgtg taaaggttgt cgtctacccc
    16201 taaaaggtca ataaacagtg tttccagtct aggctgttga tcccatttaa ctttttcgat
    16261 acgttctttt actgggttga aaacattctt tctagccaca tttactactg ctttgtgtat
    16321 tttgttgtca tcaaaaacta cattgtagtt ttcttcaata tatgccgtta aggcatcctc
    16381 aaaactgtct tcccattctc cagcggtacg attgatccaa ggagaatctt ttaataggtg
    16441 aatgtatccg ctaaattcgt tataagcaat tgttccagct aaattgctat cattggttaa
    16501 aatcaattct gcattcacaa cactgttact tttaatctga tcgtctttat tgcatcttaa
    16561 tcgtgacatc caggttttca attcaatgat attgtcttca cgctcgctgg cttcgggaaa
    16621 gtctaaaata ctatctaatt tatgcttatg agccatgagc tttccccctc aaatcttttt
    16681 taatcatcga tcgaataatt tgattcactt ctttatcttt tagcggtggt tcattgtacg
    16741 catttgctgt ataaatcaat tctgctaaag tttgattatc tacaccgtgc catagtaacc
    16801 aaccgcataa cttagttaaa tgatcgtttc tactaccttc gccagcgcct tgaacaatca
    16861 tatctaattt ttttgctgtc cctgtttttg ctacgggtcg gtattgagtc tttatttctt
    16921 gcggtttttt tagcagtagg ttcactagcc aatcgggagc tggtgcaata ttatttagag
    16981 atacgttttc aataggttca tatgctctcc caacacttct tgaaataatc atagttggcg
    17041 ctattggaac tcctttagtg ataatatcaa tcccgcttaa acttttacct ttatcatcac
    17101 gcccgaagat atcagcaaat gcacctgttt cttgttggat aattacacca tcaggaattt
    17161 taaagaagaa atggattcct ttacctggtg tgatttccgt ataagtagaa ggaaatggat
    17221 catacattga agcaagtttt ttataattct caacgccgtt tcgtgtttca gaatgttgat
    17281 ctaggtcaac taccaccatt tgatgatctt caagcattaa cccaatattt ctagttgcat
    17341 cttccgtcca ccattcatta atttgattag aatcgttagt tgcatcattc caaccactag
    17401 agccttttat tggaatttta gttccaggag ataagggata aattgaccat cccgactttt
    17461 gtaattccgt tgctgcttga taattatttg ccaataaacc tctcctcctt tttcaaaata
    17521 ctggtataaa tcattcagac acctccatga gcttcaactg ctcaagttcg tatttcaacc
    17581 catcccgaac aaattcagca ccttttaatt gattctgata ctttacccat gtattaaatg
    17641 ttttcaccct tgcttcttgc atatcagtaa tgtttaacca tgcggttaaa atctgatttt
    17701 ttagctcgtt gatttctcga caaattattt tgtgttctgc atgttttttg agtacataca
    17761 aaactgttag tttttcattg aatgaatttc caaagtaggt tttcccgata ttattttcta
    17821 gttcagcgat cccttgatta atttcctcta attcaaggct atcgagttgg tttaatagtt
    17881 ttgttttgta catctactta tgccctccag ttcttaattg atagtcgtac tcgtcataag
    17941 cagtgaagag atataacgct aatcctgcaa agatagctat ttttgcaatt aatggtaata
    18001 agccgaaaat tccgccgatt aaaatgcttc ctataattaa gtttcgtttc ataatggttc
    18061 cctccgattt tttaatatgg tataatcgga acaaagaata tcgggtttta tcccaattaa
    18121 gttgccttta tcctgtccgc caagactgtt atggataagg ctttttcttt gttctcgatc
    18181 atgttaatgc tcctaagttg tctgcaattc agcagaacga ttataaattt tctcactcac
    18241 tgaaaactgc aacccatatt tttctttaac agtcattaat tctactgtct catctaaaat
    18301 aggttcacga tcaaccaaca tttcaggagt catatctttc ttttttacca ttttggaatg
    18361 accataaata gttgatacag ctttattggc tatcacattc gctttaatgt agtctactgg
    18421 ttttggttca gtaatagcgt ggcttaattt agtcattgct tctttttggt gttctttgtc
    18481 tagcatgcgg aatacttgaa agccttcaag tcctgtggac tggcgtagtt ctttgatgat
    18541 gtcgaaaatc cagtcttgaa atgcctcggc ttctttttta cggcttttga atgccatgcg
    18601 gtaaatattt tttttcgttg atgatgttta catcttgtgt tctgcccaat gaatcgatga
    18661 ccttactagt agtaacccca tctttaggca aagagtgaat ggctctagta acctgtttaa
    18721 gacctaacgc ttccgcaaca tctttcgcca ctgcccacca ttcatcatta atatcgacaa
    18781 atcttataat gtgtccgttc caaatttcag ttttcattca acgtcctcca gttcaagccc
    18841 tagcaagctc gctaacaact caacttgctg atacaaaatt tcttgatact cttcaataga
    18901 aagtcgttct ttaaactctg cttgtactaa atcgttcaag ccacgcaata ctccgcttac
    18961 cgttggttct tgtggtagca atttttgata gtccatttgt tcgtacaaac attcaaacgt
    19021 ttcttctagc gctttgcctt ccaagtttgc taattctgcc gctgtgtctt ctgtaagaat
    19081 tgttgataat actttttcga ttgctttatt ttgtgtcgat tccattatta tttcctccta
    19141 ttaatccctt tgcccccacg gcttgcgttt atttttggta ttcttccata aagcgatcac
    19201 attcatcttg tgaaattctt acgactccat caactataat caactttaaa ccgttaggaa
    19261 catatttctg catcatagta ttgtatgaaa cgttcatata cttagcagct tgtgccttgt
    19321 tcatatattt tggaaactgg tacttttttt ctaataatga aagtaacgtt ccttgagttt
    19381 gctctacttg ttgaacaact cgatcgtcaa taattttaaa agctggatca tcaagcgtga
    19441 gtttgtcaat ttttaattcc atatttatcc cccctcgtta gcatacattt ctatacaaag
    19501 attttaaaaa aatgcaagag gctcttgaat acatttctat acctcttata aactcaatat
    19561 accatcatac cttttcattt gcaagaattt taacatactt ttttgaactt ttatgtttca
    19621 cacatattta aatcgtgata tagtagagaa aatatatgaa tggtggtgaa aatgtggcta
    19681 gaaaaaaata ccccgaagca gtggagacag gacaaagaat tagactggct agaattgaaa
    19741 agggactctc aatggaacaa cttggaggta aattatcccc accggccagt aaaggtgccg
    19801 ttagtaattg ggaaaatggc tataatcttc ccaataacac caggcttaaa caactttcaa
    19861 aggtattaga tgtatccact acctatctat taaacggatc ttatacatta agagatatgc
    19921 atatgatgcc cgaaattaag cgaaatgtgc ttgaaaagca attgaaaaaa actattgaaa
    19981 aaaataactc tataattgat tctaatcttt ctaatgctat caataacatc gaagaagata
    20041 acctgactgt ctcacagaag atttatttaa caaacgcaat aaaatatctt aaaacaggat
    20101 ctcgaattga atttcctgac gggagtaccc caattgctta tctcgcagct cttctaaata
    20161 cgcttttacg atatgactca ttaattaatg attcagaaaa aaatacagat gagaaaaaag
    20221 agtatcaaga attttatgaa aatgatcttc taaaagagtt cacagctctt gttactgagc
    20281 taaataaaca ctattcaaaa taataataaa ctgcctcctc caaccgcccc cacggcttgc
    20341 tacactatgg agggaaaact attatggcaa cattcaagca atatgaaact aaaaaaggta
    20401 aattttggct ctatgaagcc tatcttggaa tgaacaaaat gactggcaaa cctgataagg
    20461 ttcgtagacg aggttacaaa acaaaaaaag aagcccagct tgccttgtca cgcttgcaag
    20521 tggactacga taaaaacggt attaaaaaag cgaacaacga aacgtttaaa gaagtttatg
    20581 acttgtggct agaaacatat aaaaccactg tccgagaagc caccttcatg aaaactgaaa
    20641 tcaagtttag aaagtggatt cttccaaaat atggaaatct tcgtgtgaat gaagtcacgg
    20701 taaaaaaagc ccaacaaatt gtaaatagct gggctgaaac tactgaccaa tacaaggtac
    20761 ttcattcaac ttctaagcgt atttttagct acgctatcaa tcttgggatt attgagacta
    20821 atccactaga caacgtatta atgcctaaaa gaaccaccac taagcataaa gaaaaggtca
    20881 aagtttacag taaagaacaa ctgatcacct tgtttgatta cctaaacagc aaacctgaaa
    20941 cttatcgaaa cgactttgat aaaacgttac tacgctttct cttttatagc ggggttagga
    21001 tcagtgaagc ccttgcattg aattggtcag acattgattt tcaagaaaat acggtcacca
    21061 tcaataaaac actaagccaa tccaaaaatg gctataaaat cagtgatcct aaaactgatt
    21121 gtagtgctgg ggttcttcca ctagatgaaa gaacaatttc tatattaaaa aaatggcaga
    21181 ttaatcagcg gaaatatatg ctgacacttg gtgtaactga tccgactatg attttttgtg
    21241 gtatctataa agaactaatc acacaccacg ctatttatgc aagattgatt actatttgtg
    21301 aaaaagctat ggttccattt ctcggtgtgc atgtagcaag acacactcat gcttcaatgc
    21361 tcttggacgc tggagctact atggtagaag ttcaaactag attacggcat acaaaaatta
    21421 gtcagacgat cgattgttac ggtcacttgg caaaagaaac gaaagaaaag actgttgaaa
    21481 aattagtaca acacttaaat attgactaga tggcatacaa tatggcatac agtaaaaaat
    21541 aaaaaaagga gaaaccctat catatcaagg tttctcctag ctttatagtc tattcttctt
    21601 cttccgccat gaagttaagc tcattaaaga taattgacga tagttggtat ttcaacgatt
    21661 tttgaataga actaatcact ttattaagtc taattcacct taagtggttt tttagtcgta
    21721 ttcaaaaacg tattcaagtt gcgtggcgtg cttttgataa gaaatcgtaa gaactagtta
    21781 atcaaacgac ttataaataa tgtaacacca ctgattaatc agctatatcc aaaaaaacaa
    21841 gcattcattc agatacatca atctctatag aatacaaagc tttcattatc tcaaaccatt
    21901 accacaactt ggcaagtcgg ttaattgaag aacacctcat tctccactta tagcagtgga
    21961 gcaacaagtt gagctattgt tgcaacagaa gctaagaatt ctgtactcca tttaagtttt
    22021 tttagactat ctaccgcaaa cctcagcata gtttttttag gcttttcttt aatagcttca
    22081 tctttaatgg tttcgatata ctcctgagcc tcttctacct cttcttcagg taaggaatca
    22141 ttcaaattct ccaaaagcaa atcaagcttt gataataatt ccgacatatc atcaatagaa
    22201 acaatcatat tattattgtg gccttgctgt atattagtgt tgtaactatt tccaaatata
    22261 tttactttat tatatattga attgttgctc atatagtcct ccagtcgtcc tgctgcatta
    22321 cttgcatctt gtaacgtcac aaaaaaatct ctcccatgtt ggtaaattga ttcaacgatt
    22381 tcaaattctg gatttccata ttcgcgccat tcgtattctt ccttaaagta ttttccgtaa
    22441 ctatggttaa attgcacaga atttttacca attttcctta gaagttttat ctctctctca
    22501 gaaataaaat tttcctcaaa agctgattca actatctgaa aatcactaag aaatgttcct
    22561 aaacagaaga ttgattcatt aatagtttgt tgaaaattcc atggtatttt gttccaaaaa
    22621 ccattcttaa ttaaagcctc ttttttttca acaatctgtg attttacatt ttctgtatat
    22681 atcttccaat tattgacgtc ttccttaatg tccgaaatcg attgaccttc ataagaagtc
    22741 cctccaccaa aaagtccttg aaccatttta tctcatcctc tctataataa ttactctaaa
    22801 tccaatactt gctacaaatg atagcatttc gtgtggtaac atgattgcca tcgttcctgt
    22861 tttatttact ctgacttcaa tacttggttt gttgatgaaa ttttaccaat tgattccatc
    22921 gatcgtttca tggaatctgt atttactatt ttcattgatt gtagagattg atttattccc
    22981 tctgttgcac ttctgatacc tttagtatca aagttttgca tgattgctcg ctgagctgat
    23041 aaagctgata atgtttgacc tattgactct atcgacctct tcatggaatc tgtatttaat
    23101 atttttattg attgtagaga ttgatttatt ccctctgttg cccttctgat accttcagta
    23161 tcaaagttct gcataatttc ttgctggact gataatgttt gacctattga ctccatcgac
    23221 tttttcatgg aatctgtatt taatactttc attgctgata gagactctga caatttagta
    23281 acacctgcta aatcgatatt actaaaataa tttccaatat atccttcaaa atcatctgac
    23341 atatcactaa ggtcctcacc agcaatatct ccagccaata ttttttgaaa agctttctct
    23401 agttgatttg tatatctctt caatttcttt attaattttt ggtataaagc catttctatt
    23461 tctttgttat ggcttacaat attcctacct tctctgatta aactcatgtc tgtttgaatt
    23521 tcttctagct taacatccat atatttacta aatacttcac tccacaaaga atatgaatca
    23581 gatatagtgt gactgttatc ttgaaataac ttcaataatt gatcctgaga tatttcttta
    23641 taccttacaa tattctcttc attttcttgt gtaactaaaa tataattttc tccaaagaga
//
LOCUS       DAYYGS010000042        23439 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000042 DAYYGS010000000
VERSION     DAYYGS010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23439
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.042"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1300)
                     /locus_tag="ACHA44_001544"
     CDS             complement(<1..1300)
                     /locus_tag="ACHA44_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFM8904822.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILL"
     gene            complement(1324..2055)
                     /locus_tag="ACHA44_001545"
     CDS             complement(1324..2055)
                     /locus_tag="ACHA44_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904823.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(2073..2468)
                     /locus_tag="ACHA44_001546"
     CDS             complement(2073..2468)
                     /locus_tag="ACHA44_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFM8904824.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(2570..4207)
                     /locus_tag="ACHA44_001547"
     CDS             complement(2570..4207)
                     /locus_tag="ACHA44_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFM8904825.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(4242..5576)
                     /gene="gmhB"
                     /locus_tag="ACHA44_001548"
     CDS             complement(4242..5576)
                     /gene="gmhB"
                     /locus_tag="ACHA44_001548"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFM8904826.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(5618..6727)
                     /locus_tag="ACHA44_001549"
     CDS             complement(5618..6727)
                     /locus_tag="ACHA44_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WavE lipopolysaccharide synthesis family
                     protein"
                     /protein_id="HFM8904827.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(6752..7987)
                     /locus_tag="ACHA44_001550"
     CDS             complement(6752..7987)
                     /locus_tag="ACHA44_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904828.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(8018..9124)
                     /locus_tag="ACHA44_001551"
     CDS             complement(8018..9124)
                     /locus_tag="ACHA44_001551"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8904829.1"
                     /translation="MKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRDVPPLDPKI
                     KSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYEGGLYVLCK
                     RHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAEFPELNAKS
                     YVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMDVRKKVHFT
                     IAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAIFPYKEIYG
                     SGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKYVNLSPAEK
                     SKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(9135..9908)
                     /locus_tag="ACHA44_001552"
     CDS             complement(9135..9908)
                     /locus_tag="ACHA44_001552"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HFM8904830.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(9922..10377)
                     /locus_tag="ACHA44_001553"
     CDS             complement(9922..10377)
                     /locus_tag="ACHA44_001553"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFM8904831.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(10799..11905)
                     /locus_tag="ACHA44_001554"
     CDS             complement(10799..11905)
                     /locus_tag="ACHA44_001554"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFM8904832.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(11923..12684)
                     /locus_tag="ACHA44_001555"
     CDS             complement(11923..12684)
                     /locus_tag="ACHA44_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HFM8904833.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(12760..13758)
                     /locus_tag="ACHA44_001556"
     CDS             complement(12760..13758)
                     /locus_tag="ACHA44_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8904834.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(13777..14430)
                     /locus_tag="ACHA44_001557"
     CDS             complement(13777..14430)
                     /locus_tag="ACHA44_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8904835.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(14440..15450)
                     /locus_tag="ACHA44_001558"
     CDS             complement(14440..15450)
                     /locus_tag="ACHA44_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HFM8904836.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(15469..16719)
                     /locus_tag="ACHA44_001559"
     CDS             complement(15469..16719)
                     /locus_tag="ACHA44_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HFM8904837.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(17226..17506)
                     /locus_tag="ACHA44_001560"
                     /pseudo
     CDS             complement(17226..17506)
                     /locus_tag="ACHA44_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697748.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(18065..18175)
                     /locus_tag="ACHA44_001561"
     CDS             complement(18065..18175)
                     /locus_tag="ACHA44_001561"
                     /inference="COORDINATES: protein motif:HMM:NF020874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HFM8904838.1"
                     /translation="MNPNGYEWLRAKGSYPVPKYCNYSETLMEWYQLIIT"
     gene            complement(18581..19270)
                     /locus_tag="ACHA44_001562"
     CDS             complement(18581..19270)
                     /locus_tag="ACHA44_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HFM8904839.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(19315..20079)
                     /locus_tag="ACHA44_001563"
     CDS             complement(19315..20079)
                     /locus_tag="ACHA44_001563"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HFM8904840.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(20107..20805)
                     /locus_tag="ACHA44_001564"
     CDS             complement(20107..20805)
                     /locus_tag="ACHA44_001564"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HFM8904841.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(20818..21597)
                     /locus_tag="ACHA44_001565"
     CDS             complement(20818..21597)
                     /locus_tag="ACHA44_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HFM8904842.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(21613..22557)
                     /locus_tag="ACHA44_001566"
     CDS             complement(21613..22557)
                     /locus_tag="ACHA44_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8904843.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(22598..23377)
                     /locus_tag="ACHA44_001567"
     CDS             complement(22598..23377)
                     /locus_tag="ACHA44_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904844.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
BASE COUNT         7131 a         5089 c         3470 g         7749 t
ORIGIN      
        1 tcaaaagtat tcctctgaca tcatatatgt tttttccatc caatagttct ttgcaaactt
       61 ttctataaaa aatataatgg atgacgcaga aaaacatata tgagaataca gttgtgtatg
      121 ctaccgcctg atacccatat aactttataa aaacataatt tagtacaata ttcataattg
      181 cacacaaaac cgatggaata acgacagtga gtttcttttc aaagtattcc tgtactctag
      241 caaacagctg aaacatatag ttgaaataaa taccgataca tataggtaca attaaattaa
      301 cactgtcgat atatttctgc ccaccaaata tcaacacaat ttctttacta aatagcatta
      361 atgcaaaaag catagccgct accaaaccaa gaacttcatt agctcttttt gatagtaaat
      421 catattctcc tgacttgatt ttttgatact gataaggtgc aaaagtggaa ttgacagaat
      481 ttgtaacaag gttaattaga gcaccaatag aatatgccac gctgtaaata gcaacatctg
      541 acgaaccaca taagtaattt atcataattt tatcagaacc attcagaata aattcagata
      601 aatagtgtgg catcaacgat acacaaaata ctactgcgaa attccacact tcattatcct
      661 taaaatcctt acatcttcta aataccgata tatacacagc cacgccaaca acaccagtca
      721 ctaggacatt cattaaggct ctcgcctcag cggtaggaga tataaacaga accgctaaac
      781 agccaactac agatgagcca acagtaactg caagggtagc tactaataaa gtcttataat
      841 catatatata tcgtttgtgt aaagaccaac attgtatttc tgcctgaaat ataaaatatg
      901 agaacataca gacaactaga actggtgtca atcctaaaat ttctgccaat atatccttaa
      961 taaaaatagc aataacaaac caaaaaatag tcagtactaa agtcaatccg gatactgtcg
     1021 aaagaatatg ttcttgatta tcatatttta cataaagatt catcatggct ttgaacaaac
     1081 taagcgatgt aatcatgagc aataaggatt cccacgatag ataagtgctg tataaaccgt
     1141 attgttctgt agacaaaact ctagtaaaaa ttggggttgt aatcattgaa acccctctct
     1201 gtaaaaaacc acataccata aaccataatg cagccttaac aggaagtgga gtattcttat
     1261 atctgcttaa tgtttttaca attagattat tactggtcac tgctattttc tcctaaacac
     1321 tatttattct tgattcaaaa acacattgag ctcttctgga gtaccaagca catgaacctt
     1381 tttcgcatct actatgctaa tagtcacctt taaccctttt tttatcataa agttatataa
     1441 tggtgctata tatttttcat tcttctccat attagaggag tctgcataat actcttcata
     1501 taaagcttta tacagctttg ccgatgagaa ataatacgca ccaagagtac aattatcaga
     1561 aatgcgaact ttttcgcgga cttctattac ttcatttcga tcgttaagct ttgcaaaact
     1621 ccaatgatcg ccatcagcat gaaagcatgg aatatgtcca tctccacaaa tatcttcgta
     1681 tttcatctca tttgattcca cgtatgtatc aatattgtaa accataatct cattgtcttg
     1741 attgcaataa ggaatagcca acatacatgt agttgcttga ccatctgtca tatagtctat
     1801 ttcaatcact tctacgtttt caataccaaa gtaagcacaa tgctccttta taaaagctga
     1861 agcgttgtct tcctgacgaa caacaaaaat atacttatca acatttttat tgtatccaac
     1921 caaactatcc atggcccaat caaataaagt tcttcctttg gcattaatca tatatttagg
     1981 aaccgcataa ccaatttttc gaaaacgact tcccaaacct gccatcgtaa tgataactgt
     2041 catctttttt tccatagaac taacctcctt atttagaatc ttttttttga actgttgcag
     2101 atttcattta attcatctac agagcatctc aaaaactcat caggtctaac cgttcgatca
     2161 tctacataga agcctttatg ccccggccaa ggcttaccat acaggatttc atcatacgga
     2221 atgtcccatt tatccaacca cgccaagagc acttttgcag tattagcatt tatcattccc
     2281 aaatttcctt tataggaatt aacattgcgt gaggtgaaca atgtaatttt agcaccattc
     2341 tccttataga atctcagttt ttcaaccata ttctcatacg gcaccaaatc ctcatatcgc
     2401 tcatccttct tctttatcgg acacaaagtc ccatcgatat caaacacaaa agaatattca
     2461 ctatacattt ttctcttccc ttctattgta ttaattaata taatcgtgcc ttaatgccta
     2521 cattgctaaa acagtattgt aaccgttatt cattaaggca caatttatat tagagtataa
     2581 ctttattact tactcttgca agaagttcca tacctgtagc aagcatcact atttggtgat
     2641 caaaactttc accgtgtaat ggaatcatac taagaaatag caacgcttct atcaatacaa
     2701 tcttatcaaa atcgtcctta atctcgtccg aaaagaccga cttaaacaat tcaaataaat
     2761 cgtaatctct tacacgcgta ttaattgtat aatctatcac tgcacactcg gtatcatatt
     2821 ctacagtaaa tagatccttg atgatataat catatttacc atctaaacta tgaaatagtt
     2881 ttgctaattc atatctgctg tccccataaa tatcatattt tccaaatttt cccctaggat
     2941 caataacctt aacaaaggaa caattggaat ctaccatcat attagcaaaa cacaaatctc
     3001 catgtataat acaaaattca tttacatcat agagcatact tgggataaga ttctttagaa
     3061 gttcaattac accggttaaa gaaataaact tacgaccatt aatttgaatt tgtttctcaa
     3121 aaaaagctga aaatctttta tcttcagaca atttttgaaa tctctgcatg gtttttcgca
     3181 aatacatatc ttccaatgac tctctaatct tttcatcttt taaggtgtat cgcttaaaat
     3241 cgctacagat aaactttata cggctaaaca gatcatgcca ctggttatat tttaaatcac
     3301 catataagta taactcatga accgtatgat atgcataata ttccatatca acatagggag
     3361 ctacgtaaga tgtagaataa tcaaaaattc taggtcttac atattcaata tcagcaggaa
     3421 gttttaaata ccactttatt tctccaataa atttatcctt gtcatcgcta gttttcttca
     3481 atatccctcg atctttatct atagagatat gattaaactc tcgtgcttta acttccaact
     3541 ttgtattgta atatttatcg atgtgtccaa tatcaaacca cttttctgtt cttacggcct
     3601 ttagcggaca acgttcgctg tatagctgta aagcccgata aaatgagttt attcccccct
     3661 gtttccgact ctcaactgcc tctcttaagc aactcttcag aagttttgac gaagaaaaca
     3721 taaatacgcc aataaataat ttttcacgtt tatcggatac gtaagcttcc tctttatcgt
     3781 ggatatcagt aattattcct tcattaattt taaagtatgt ccaagtactc gacacaaaat
     3841 cttcagaata atagaatgta tccatttcta tatcagacat actctccata acaattgtat
     3901 ctgcgaagtt aattagaaca ggctcatctt catctgataa cccaaaatac acggtttgcc
     3961 ctagatcccc taattcagga aggtcacata tatgaattct ggaatcatga tagtttttca
     4021 gtcttctatg aaccttatcc gctttttcat aacagataat atccaatgaa tcgcagattg
     4081 cattatactg attatacaaa taatcaaaga caattttttt attaattgga tagataattg
     4141 ctggtaacat tcctatactc tgcaattctt tttcaacaac ttttgctgct ggaattaata
     4201 ccttcatcta attactcctt ttacaagttt cactaaacca attattgcat agtgcataaa
     4261 tgatttttaa caaattctaa caatgaacag acactatagt cttggccata attagcaggc
     4321 aaggtgatgt cttctgtaat cagtactgtc ttacaaccag cattttttcc agcaattaca
     4381 tcactttcac tatctccaac cataaatgat tgacttaggt ttatgttaaa atcctttgat
     4441 gcttctaata acagtccagg ttttggcttg cgacaattgc aatcaatttt taattctata
     4501 acctctcctg catatccttt atggggatgg tgcggacagt aataaatacc gtttaaaaat
     4561 ccaccagcat gccctagcat tgtttcaagc ttattgtgaa tactattaag ctcatcaaca
     4621 gtaacttcac ctcttgcaat tacaggctga tttgttataa caatacacag aaaacctaga
     4681 gaattaataa ttttaattgc ctccgatact ccatcaatta actcaaattc ttctgcattt
     4741 ctcaaaaaac caacgtattt attaatcgtt ccatctctat ctaaaaagat tgctttttgt
     4801 ggattggata gatttttacc aacaataatt cccttttgaa agtccttaat tacagctcta
     4861 tatcggtccg gtgttcccat atctttaaca tactctggag aatcgtagca atacatacgc
     4921 cctgtgttag ccaatggttt cagaatcatt ctatctaaat caattttgta tggtagcccc
     4981 ccaccttcaa ctattgattt atttacaatt tccattaatg gattttctct caacaccttc
     5041 ggatttataa catgaagacc tgcatttact cggtttttat aatattcagg tctttgatct
     5101 tccttcgcta accattgaag cactttctta ttgtcatcgg atataattaa tccactatca
     5161 tacggatgtg agttaggatg cgtaaacaaa gtaacatctg cgttattctc aacatgaaat
     5221 ctaatcatct gcctgaaatc aacatcaaat actgcatcag cattcaaaag taaaaacggt
     5281 tcatcaccca gttcatccaa caaaaaatac aatgcacctg cattgccaag tggagttttt
     5341 tcaataaaat atttgatttt aattcctaat tttgaaccat ctccgaaatg ctcctctatt
     5401 ttttcatgca aataggaaac agtcaaaata atatcattaa agccttgatc tctaagcgaa
     5461 cacaattctc gttctaaaac tggaatgcca tctataggaa tcattggctt aggaatatcc
     5521 ggaaacaatt ccgaaattcg gcttcctcgt ccgcccgcca taattactgt tttcataata
     5581 tgcccttccc taaagtactt tttactataa aagttaatta ctcttcccaa acaaagtcca
     5641 tatatttttc atattctgct ttgtattcgt attttccagt gtacaaattc aaccaattta
     5701 taaaatcaaa attatagcag tgaagtgcat cttggctatc atcaatataa tacgtaccat
     5761 aaatagtatt atttgaataa cgacctacat atttatgcca gtacaaatca atttgatttt
     5821 tgttaatagt aataacatgg ttttttacaa actcccaata tgccctaata gagcactcct
     5881 cattgccccc cattcgtgaa atatagcttc gaagaagctg aacttctgga gctaaattat
     5941 tttcagatat ctcccttctt gagactcctc tagacacagc tcccttttct ctcacatcac
     6001 taggaatcga gaagaggttt atcatatcat caacatcacc aaaatacagg aagtctgaca
     6061 aacaatacgg gaaaaacatg tctccatacg gcattgatag taacagtaat cgctgatttt
     6121 gtattaattt ccaatcatct tcgttattaa caggatactg ttcaagtaaa ctgcagaaaa
     6181 acgccattgc atttggatgg tatattcttt gatctgttct agttttacaa acatactttg
     6241 caccaagctg ctttgctctg actattccag gcaaagtgtt tccaacttga taattaatat
     6301 ttaagtgtcc aggcttttca ggtttctcac tacaaataac tataactcca agatcctcta
     6361 tccttttcaa tgccttaata tcttcgtcct tccatgtcga aagaataatt aaagcatttg
     6421 gataacattt cttatagtag gccagagtat tataagagaa atcatccttt aaacatagtg
     6481 gcccctgcat tacaattgct acatctttgc aacaattaac taaacccatc actggaacat
     6541 cagacatgtc ctttgagcgc aattgaaacg ctgcataaac ttcagaattt tcttctactg
     6601 ttgcacaatc agcagtcaat ttccaagata cgagtttact tatattatga ccaaacatgt
     6661 tcctaaacac atatggtaaa ttttcgtact tcttcaatat gttatataaa atctctttct
     6721 tattcatgag atgcactcct attaatattt cctattctat gttagccaca cttctacatt
     6781 cttgatagat gtattctata accgctaagc atacaggaat agctggagta aatatcggat
     6841 aaaaccagtc tatattaatc cctgttagca agacatttaa tgtaattcca aatgctaaat
     6901 attgatagcc agtcataccc acttttgatg caagtaatgc tgttttgaac ccttttataa
     6961 taaacagaat atcaaatata agtataccta caccatattg agtaattgta tagaaaaatc
     7021 caatatcaga taggtgtcct ctaactcctc ctggtaatat aaacgtaaat tcctcaccag
     7081 taagaatacc agtaccgaac aaaggcgatt gcaaaaattt aggaataaaa aaccaaactg
     7141 aagcaaatcg agattcagca gatcctgcat attcacctgt actgtttgtg aaagtttcaa
     7201 caaatgttga gaacatggtc gaattaataa atatgactac aagcaaccca gctaaaaccc
     7261 acattgcaat ttttcttttc gaagaacctt tcttaaaaag gatatagcaa ccaagtacaa
     7321 taatttgata gacaaggaca gatctagaac catgaataac agcagaatat gccagtgcgc
     7381 tagcaatcag taaataatat tttacttttc ttctagctac aacttgtcgc tcaatcatat
     7441 acaaagcaat cggtattatt ataattgtaa aacatggtgg attaattctt aaaaagcctc
     7501 cacgatacca attttccggg gcacactcac gatatatgtt ttcatagaga agtactcctg
     7561 aaaatttctg aataagtgaa atagatgttc taagaatgta tgaaagaaat gttacaccac
     7621 atatcgtatt tagcattgaa tccaaattcc acgcttggtt aattaaagta cagaacaatg
     7681 gatacgccaa taatacatag tataaactca tactttccct agatttaacc catattgtct
     7741 tatctgagta gatgtaagct gctattataa caacacaaat cagtaaagtg cagaaattta
     7801 aatatctatt ttttctaaat gctggtaatg aggctattaa caaacatact attgctagaa
     7861 tgactgtcac aagtctaacg ttcatcgttt ctcccaacca gccgcccaaa ggcttcaata
     7921 tgcttgatga gataatcatt agaaagacca ttagaaactg tatgattttc catttactaa
     7981 tctgcatgtt aattattatt cctttctaat tgttttacta ctcattcaaa atttgtttgt
     8041 atgcctcgta tgtgagtttt gcagatgccg cccaggtata cttgcggttt acaagctcat
     8101 atatatcatc ctctattttg cttttctcag ctgggctaag gttcacatac tttacaattg
     8161 tgtctgctag gctcttagaa ttcaaatttt cgaaaagtaa ccctatcttt ccttcctcgg
     8221 tttcttccat aaaagcaggt acattactaa caatagttgg taagcaataa gtgtatgcca
     8281 taagaagcgc tccacttcca tagatttctt tatatggaaa tattgcggca tctgcatttc
     8341 tgaacatact gtccatttct tcatcagaaa ttctctctgg cttatataga acacaatctg
     8401 aaacctttaa atcttcaatc atttttttat agtcagtact atcctgttta ggatgttgtt
     8461 gacctgctat ggtaaaatga actttctttc ttacatccat tggaagttct gtgattgcat
     8521 taagcagtac atcaattccc ttatatggac gaatttgtcc aaacaagata aattcaaact
     8581 tatctttatc atacgctcta gatatatgtt taacatctcc atagatagca tgtggacaaa
     8641 catatgactt tgcatttaat tctgggaatt cagcatataa atgctgttct gttactcgat
     8701 tgtgaacaat gaaaccatca caatttgaat atatattaaa gtgctcttgg cgatcctttt
     8761 cacgaacctc atgtggtaac acattatgaa ctgtcataat taatttttta tagtgtctct
     8821 tacataaaac gtacaatccg ccttcatatt tgagactttt catcaattgc aaatgaacaa
     8881 tatcataatc accaaatctt atttccttcc aaattaagaa caagcttttc agaaaaacaa
     8941 gtgccgaaaa cactttattt gtacttttat gatacaaaat atgtttgctc ttaattttag
     9001 gatctaaagg aggaacatca cgcttacaca gcaatgttaa atcaacatat tgattaagtt
     9061 ctttagaaag atcaaacgaa taccaattct ctggcatttg gctttcacac atcaatacct
     9121 tcataatact caccttataa ccaatcaaca gaaaattctg ctttcagttt tcctttaatt
     9181 ttacgttttg ctataaacat ttccagtatc tctcgcattt tcgtctgata ttttgtcgtt
     9241 ttaccataaa gcgagtgatt gtcccctgtt cctaatacat atactgctgc tttgaatgga
     9301 agaggtttta atggacgccc ttgctcttca catgctggaa caacgccccc atgtcgtttt
     9361 ctaatgatcc atggattatc atcacaattt gctgtcataa cttccggata tgcctcaggc
     9421 aggtcttcca ctgcatattt tactatacac gcacttcctg atgagaactt tggtagaacc
     9481 ttcatataat cgtctccaac aaagtacacg tatcccgttt tgacataaaa tccattttcc
     9541 tggggatggg aattcacata ttccgccaaa tgagaactta caagatcatc tgcgtcaacc
     9601 atcatagcga aaccgccgcc caactctcgc aaacgcattg cgagagcatg tgttttataa
     9661 cccttatcaa tcattttttc gtcttgattt gtgggaatag gggcatcctt ttcgctaact
     9721 tgaataaatt caactctgtc atcatattca tgcgtgagtt taggaatttc atggcaagca
     9781 acaatgattt tgaaatcagg atcagtctga ttataacacg accataatgt tcggttaaat
     9841 aaggtactaa ccatctccca attgtttgct gccttcgcac tcattaaagg aataacaaaa
     9901 tacaacatac aatctctcct tttagtaacg attcaaacta caataatcat ttacatcaat
     9961 atcttttttc aatactctag ccggaacgcc tcctaccgaa caattaggtt caacatcatt
    10021 aatgaccaca gcattagccc ctactattac attgtcccct attgtgatat tacctaaaat
    10081 ttttgcgcca gtagcgatat atacattgtt tccaattgta ggcataccag gcttattgga
    10141 tctaccacca gtcagtacat tcgttccgat tataacgtga ttacctattt ttgtatcttt
    10201 tccaattaca caacctatgc caccgtataa gaacttacaa ttatctccaa tttctgtttg
    10261 atatgaaatt gagctattat gaagtacata aattagtgcc tttacaatct tggcaaaaaa
    10321 ctccattttg tgtaagtaaa accatcgttc tactttgtat aatttatatg cattcatgtt
    10381 ttatctcccc atctttttag caggtacccc ccacacttcg tagggtggaa tagacctcaa
    10441 taccactgta tttgcagcaa ctacagcacc ttccccaacg cataattctg tgttagattt
    10501 acaaagaatt ttagcaccca cacataaaac agcgttcttt tttactcgaa tgatgatacg
    10561 ttcattacta tctgctcaac cacacgtcac attctgatag actttaacac catcttcaag
    10621 gattgtgtca gaatgaatca cagtgcctac agaattatga acaaattcaa cagagttccc
    10681 tatctgtgca cctttaccaa tacaacaacc aagaattttg gctatttttc tcgtgcaatt
    10741 atcaactatg tttaaagcat atcgattcct caatgccatt aatttcgtta cttccatatc
    10801 atttatcacc ttccaacatg ctaagcataa tttctcccga ctttgaccat ctaaattctg
    10861 tagttacacg ctgatatgct gcatctatga tatcgttggt aaatcgtccg tcttttaaga
    10921 atgtcaaaat ttcatcgtca gatttagcca acattccata tctattatcc aaaataatat
    10981 tctgtgggcc aggtgcatta acagcaataa tcccgaggcg atagtacatt gcctctagca
    11041 aaaccatacc ccaaatttca gtatagttta gatttacaaa tacatccgat aagcaatata
    11101 actcccaaat atctttgtta ggaatacgct caattatttt tactctattc tgcagtccaa
    11161 gtaaatttat ctgctgcttc gtttgctcga gtaaagcacc agttcctacc atcagcaaat
    11221 aattatgctc atcaagttta ttaaataaac taatcatatc taacggtctt ttatcctttt
    11281 caaaccgtcc tacatataaa ataacacgaa cattgcgagg aatattccat ttttctctga
    11341 gagtagtaaa atctattgct tcgtaattct tatgtaactg ttcaaaatcc acacaaacag
    11401 gagcaagttt cactttattt actcctaatt gtactaattt ttcagaaact cgatcatttt
    11461 tggcaacaca cccatttttc ttataaattt tataatttcg atgcgccaat atttcaataa
    11521 ttgtctttat atatttattg ccactttgac ttcgcgttat cccattataa ggtatatatt
    11581 ttattccatt ttttacagtc catgagtata catgaggtac tgatagttga aggtccgcaa
    11641 attgtattag aacatccgca ttcttatcaa aattctttgt tgataagaat ccatttgatc
    11701 ctacataacg acacttgata tagtgaatca tcgccgtatt gcttaattgc tctgttccaa
    11761 cttcctcatc actttttaca cttttatata caataacttc gtggcctaat tcagctaacc
    11821 ctcgagctaa tcctatttcc tgagaattat aggacccctt cttaccaaag ttattcgtca
    11881 ttgtaactac tataagtatt ttcatctatt tttattgccc ccctacttag attcaatagt
    11941 atagtagata tactttatac ctcccaagat ataccgcctc catagtctac gaggttcctt
    12001 taccagtcta aataaccact ctagactagc tttttccatc cattcaggag ctttttccaa
    12061 agtgcccgca aaaaagttaa agccggcgcc tacaccgaac attactgtgt tatggatctt
    12121 cttctggtgt tcccacatcc acatttcttg cttaggcgca ccaataccaa cccacagaaa
    12181 atctggattt aagttgttga tttcctcgca aatcttgcta tcctcttctt catttaacgg
    12241 acgaaatggt ggggaataca ttccaacaat tttgattccc gggtaatctc tttctaggtt
    12301 tgcacgaagc ttatttaaca cttcgttatc ttttccacca tagaaataat gtgtttttcc
    12361 ctgtctaacg ccttcttcaa aaaccattcc cataatatct ggtgcagcac atcgttcaca
    12421 ggtaaatcct ttgcgccgtc caattttaac aataggcata ccatctattg cagccattgt
    12481 agcctggcta tacatttcag caattttggg attttccttt gccgaaataa tggccggcac
    12541 tcccacgaga tcaacagtcg cacttaccgg agatgttggc atggaaaaaa cttcccttac
    12601 aaactcgttc tttgtagtag cacaaacacc tactattcca cacacagtga aatcacgttt
    12661 tcttttatac atattctttt ccatctcagt acatccttac tcatatgttc ttcctccgca
    12721 tcctctaatt acacggagga agaacagctt atatacttat taacccttaa tctgcttttc
    12781 aatccaattg taggattcac gaagaccctc ttccaaagaa acagtaggct cccaatccaa
    12841 aatctctttc gccttagaat aatctgcgca acgagcctta tcaccttcag gcttagaagt
    12901 atcaaagaaa ggcttgatgt ctttgccaga aatttcaatg atcttatatg caatttcctt
    12961 gatggaagta cagtgagaag ggccaatctg aatatgacca tgaccccaac ccttatccag
    13021 ggcgagcacc aatgcattta cgatatcatt gacatgaatg aaagcacgtc cctgctctcc
    13081 gctaccccac acattaaaag gctcttcagg attattgatt gctttacgaa tcagagcagg
    13141 aataacctga ctacgttcgc caaaatcagt aggtgtgcca taaacattat ggagcatcag
    13201 gatacagcac ggaattccgg tttctttttc caaatacccc atctccaact ggcccatcag
    13261 tttgctccag ccataagcag actcaggaag agcagggaac aactcctctt cacggagagg
    13321 ctcaggattc aaagtattct gtctggtcag agggaaggag catacggtac caacatagat
    13381 aacacccttg attttctcct tgccagcctt acggcaacaa tcaaaaacat tagaattgat
    13441 cagattattg attcggaaca attcgccctg gttcttaaac acatagtcaa tgccagccac
    13501 aacatcagcc agatgaacta catactcagt cgtaccaata acctttttag cctgctcata
    13561 atcagtcaga tcagcattga agaaatgagt atccaggtcg atgacaggat gcccatcttc
    13621 gtcgttcagg tactccaact ttcctctcca cagattatca gctacataaa catcccagca
    13681 gccttccttc accagtctct taacaagatt ggagccaatc ataccgcaac caccggtaac
    13741 cataattgtc tttttcattt aacattcctc caaattttat tcgtttccaa agaatttatc
    13801 ttccagcatc aaacaccaac aatgatatat cggcaagtga tactcttgaa tcatgtatgt
    13861 ttcaacttca ggagccttaa cacaaacatc tgctaccgtg gcaagttccc caccctttgc
    13921 gcccgtcaag ccaataactt ttataccgag agccctagca acaacagttg cgctcataat
    13981 attcttgcta tttccggacg tcgaaatacc aaggaatacg tcaccagatc taccaaagcc
    14041 aaacagctgc tgtgcgaaga cacccaaacc atcgacatca ttaatataag cagtagacaa
    14101 tgcttcgtgt gcgaccaaag gaattgccgt taaaccacac tcgagatttt tggctaattc
    14161 cggacctctt acagggtcaa ttgcaactaa cttatcggca aattccttac ttacaggacg
    14221 tggcgtctta aaccgcttca tcaactcgcc agcaatatgt tccgagtcag cggctgatcc
    14281 tccgtttcct gccacaagaa gtttaccacc attcaaatag cattcctcca taattaggta
    14341 tccatcgata atgtcctgtt taatcgtctc aagtgtagga tatcgtttaa tcaataagtc
    14401 aatatgttta ttaagttttt ccgctaacat tgttactcct tatccattct gtttttcaat
    14461 ataagtttcc ggcgtgtagt gaataactct agttccacca ttttcaaatt caaacggaat
    14521 atacatcaaa tcacgcatag cccaacgaac tgcatcctgt ttctcaggtt gtacatagaa
    14581 aaccaagaaa cctccaccac ctgcaccgag caactttccg cctaaagcac ctgcagccat
    14641 acccttttca tatagttcat caatgcttcc tgtactaatt gctccaccag tacctttttt
    14701 caatctccaa gtaacatcaa gctgtctacc gaaatcatct aaatttctgt tcttatcagt
    14761 aagaattgcc tcagcttcat ctacaagctc atacatcttt ttcagtctta ctctcttatc
    14821 ctctgtgcca ctgacgttgt tagccttttg aacatcagaa gaaaatctag tgaatccggt
    14881 aaagaacatc atcagattgt tgttaagctg ctttttacgt tcaggcgaaa taatgacagg
    14941 cagcacctca taaccatcca cattgaaatt aattcgattg aacccaccat aagatgcggc
    15001 aatctgatcc tgccatccac cggcttcatt acacagaact ctttcaagat aaatggcttc
    15061 atcagcaagt ttcttcttgt cagcatactt accctccagt gcataaaaag cattcaacat
    15121 accaactgca aaagaactgg atgtacccag accagagcga gcaggtaaat cagcttcgta
    15181 tgtaagccga atttcatgca tatcgagcat tttcatcgca ttacgaatcg caggatgctc
    15241 aatatcttca atgctagtta ctctctcagt acgagaataa gataactcag cgctgtaatc
    15301 aaaaaatcta ggcagatgtc tcacattcac ataacagtac ttatcaaaag tagtagaaag
    15361 aactgcaccg ccattctctt tgaaataatc ctccatatcc gttccaccgc caaaaaaaga
    15421 catacgaaac ggtgtttttg taataatcat aaatactctc tcctaatctt agtctctctg
    15481 gaagatatca cgggtatata ctttttcctt aatgtcatca aggcaagcat cgtagcgatt
    15541 tgctataaca gaatcacaca ttgccttaaa ctgttccaaa tcattcacaa ccttactgcc
    15601 aaagaaagtc tctccatctt tcaaggtcgg ctcataaatc acaacagttg cacctttagc
    15661 ttttacacgc ttcataacgc cctggataga actctggcgg aagttatcag aattggattt
    15721 catggtcaaa cggtaaacac caatcacaac aggcttttct ttgccagcat catactgatt
    15781 tgcttctgcg taattgtaat aaccagccat ctgaagtaca cgatccgcaa taaagtcctt
    15841 acgagtacgg ttagattcaa cgattgcctc gatcagattt tcaggaacat ctgcgtagtt
    15901 ggccagaagc tgcttggtat ccttcggcag gcaatagcca ccgtaaccaa aggaagggtt
    15961 gttgtaatga gtaccaatac gaggatcaag gcaaactcca ttaatgatct gctgagtgtt
    16021 caagcctttc atctctgcat aagtgtccag ctcattgaag tagctgacac gaagagccag
    16081 atatgtattg gcaaacagct taacagcttc tgcttcagta aagcccatga acaatgtgtc
    16141 cacattctct ttgattgcgc cttcctgtag cagtgtagca aaagtgtgtg catcctcaac
    16201 caaacgctca tcatccatat cggtacccac aataatacgc gaaggataca gattgtcata
    16261 cagtgcctta ctttcacgca ggaactcagg gctgaaaata atattcttgc taccagtctt
    16321 tacacgaaca gaagcggtgt atccaacagg aatggtggac ttaataacca taatggcatc
    16381 aggatttact tccatgacca gcctaataac agcctcaaca gcggatgtgt caaaatgttg
    16441 agtgcggcta ttgtagttag taggagccgc aataacaaca aaatccgcag actggtatgc
    16501 tgcctttgca tccaatgttg cggtcagatt cagttctttc tcagccaagt acttttcaat
    16561 ataatcatcc tggattggag atttacggtt attaatcagt tcaaccttct ctggaatgat
    16621 atccaccgct gtaaccttat ggtgctgtgc caacagagtg gcaatactca aaccaacata
    16681 accggtgccg gcgactgcaa tgttatactg tttcttcata atatttcctc tttccaccac
    16741 tgctgtggtc actactttct taacaaaatg ctgtttgtga acagtaacct gctatttata
    16801 taaaacagcc cgaaagctgt gttcattcat acttaaaaca gccagctcct acgccggcaa
    16861 attcaatttt tcataaatcc cctacccgtt tagggttata ttgcaagttt gagtcatttt
    16921 tattgacctc gtttggggtt ataatacatt ttcgcattga aatgtaccta cattttccac
    16981 tcaaccagtc accaaaaagt ttataaattc acttattacc ctctattctt actccaaagg
    17041 tatgtttaac atcgctttat tgacctattt tcaccccaaa tggaagtttg tttggtacat
    17101 tattggtcta ttcccacccc aaactgtcta ttttgagcga gtttttgttt tttatcattg
    17161 cacattttca aggctcaaat tctaagcgga tttactggca gggagacatt ggtaaatact
    17221 tgaacaattt cacggctttc atcatcacat cctacgataa taattctaat tggataaaga
    17281 ttatcattaa taatctactt tcacgtaaaa attctgaact aaataaaatc tattgataat
    17341 atttttttct tccacttttt gtatatccta caggtatagt ggatttaatt atccccaatg
    17401 cctctgaagt aacagacatc actaattcaa taacacattc actaccatag tttgtaggaa
    17461 caaaaatgac aacaaaatct gcttccttat gtgatgtttt tccatctgtc caaattttat
    17521 taccaatgaa atctagttta aaatccctct tagaaactag ttcactctat atttttattc
    17581 agtttcatta gttctgtttt tttgtcttca gtcaccatat acatatatat cttaattatc
    17641 tatccatttc atcctacgca ttcttgcgta aatacaattc actctgttga gctgatataa
    17701 atcattggtg gtacaaagat aaaatgataa ctgtttctat acctccaaaa attccagtaa
    17761 atatatctat agatagtttc aaaaaaaatt gtcttagttt cataatattt aagatagtgg
    17821 tacatacttt ataacaacgt acttttccaa atattatttt ccgttatgaa catccaatat
    17881 attacaaagt ttaaaagttt agatgaacta taatcttcat ttctgcacag tggataataa
    17941 attcttatag ataaaaaata gcctcttcta aagtcacaaa taccttactc ccacatttta
    18001 agtaaacact ctgcagcatt ctctttgtta taaaatgata gatattcctc aattttatct
    18061 cttattatgt tattatcagt tgataccatt ccattaatgt ttcagaataa ttacaatatt
    18121 ttgggaccgg ataactccct tttgctcgta accactcata tccgttagga ttcactaaaa
    18181 gtttaccgtt tatttttcga ttcttttttt gaaccgtccc atgaacatac cgattcaaca
    18241 agctaaatta taaaaattgg tgtgctgtca ttattttttg gaaaatttat taatggctat
    18301 caatatactt ttgtatcata ctaaatagct cgtcagttcc tatatcggat ataccaatat
    18361 taaagcattt agatcattat atttaagagt ggtatcatcc caaaatttcg aatattttct
    18421 tttatataag aataatgata ctgaattgcc ccattcacct ttcttttttt agattttcta
    18481 caagtgattc aaaccctcca taattacagt tattactttt gaaacgataa tatgaacatg
    18541 tgtaatcttt tttattaaca atccatcatt ctatccctaa ctaatatgct ccattgggct
    18601 tgacaataac ataaaacgtt tttaaaatca acctcaagtc gtacattaaa cttcgatgac
    18661 taatatactc taaatccaac tcaaccattt tatggaaact aacattattt ctatcactaa
    18721 cttgccacaa acctgaacaa ccaggtttta catacaagcg ttgaagatca tatctcgtgt
    18781 actcagctac ttccctctcc aaaggtggcc gtggaccaat tagactcata tcgcctttta
    18841 aaacattcca tagctgtggc aattcatcaa tgcttttggc acggataatt ttacctattt
    18901 tagtgacacg gggatcttct ttcatcttga acattgcacc ttctatttca ttttgatcaa
    18961 gtaattcttc caatttttct tcagcatcta aacacatcga acggaattta tacattttga
    19021 atgtttttcc atttttacca acacgtgtat ggcaaaaaaa tatcggactt cctggctctt
    19081 cttttctgat tcgataagct acatagaaca tcaaaggaga agctaaaatc aaaccaataa
    19141 tgcttcctac aatatcaatc attcttttaa aaaataaata gatatatcgt ttttcaactt
    19201 tttctttatc caaccaaact tgattaggat gtacttcact aatacgagat aataaatctt
    19261 cactttccat actttccctc tctcctcatt tcctttttat ctacgaataa agaactaaaa
    19321 taaaccaaac ttcttctttt ttacctcttc atagactgga taactgattt catccccgtt
    19381 gatcagatcc tttgcgactt gtttcatctg tactactttt tccttaccaa agtccttagc
    19441 aatctgctca tagcactctt ttaagtaaaa ggtacgctta gagaccccat gggcatcaga
    19501 agcagccatc tgcacaagat tatgtttgac catcattttg gctgttttct ggatgtcttt
    19561 tccaaataca ccgacataac ttggtgcggt gagttgggcc aagcatccca tatctaggaa
    19621 tggcagcata tgattcgggt ctttacgaaa atgcgcgttg cgttctggat ggacgatcac
    19681 tggtgtcttt cctagttgta acaactcaaa aaagagctgt tctgcatata acggcacttc
    19741 cagtgtcgga aattcgatta gaagatatgt atcatctaaa tctgtaaata aaatttcatc
    19801 tcggtcgatg tcttcgatca gtgttccggt aatacgtact tcttgtcctt ccaataacgt
    19861 caaaggcaat tgtcttttct ctaattctgc ttgtaacgaa gcgactagag aaatgacttg
    19921 tgatttttca ttgctgtatt ttccattatt atgatggggt gtgcataaaa tatgtgtgat
    19981 cccttgttga atcgcttttt cagccatggc gatactggca tctatgtttt ctgctccgtc
    20041 atcgattcct ggcaggatat ggcaatgtag atcgatcatt tctcttgtct cctctctttt
    20101 tatttcttaa ttcccgtagt aatagccagc atctttgctg tgttctgccc cattgtatac
    20161 aacaccaagg actcgcgctt gtaccatatc cagcaattct tttgctttag tcaatgattc
    20221 tttccgtgac atattttcac gtacgaccaa taaggtgcca tccgttttgg aagacatgat
    20281 ctgtgcatct gttacggcaa ctactggcgg catgtcgaag atcactacat caaatagttg
    20341 tcttgcttct gctaaaatct gatccattct tggcgaactt agtagttctg atggatttgg
    20401 tggtttaggt ccacttggca gaatcgacaa attctcgact ggtgttcgtt ggatctcatc
    20461 tgctacactt cctgatgaac taagaacggt acttaatccg cttgcgttgt tcaacttgaa
    20521 cgttttatag atgacgggct tacgtaagtc tgcatcgact agcagcaccc gttgaccaga
    20581 tttggcaaaa acaacggcaa tgtttgctgc agtggtcgat ttcccttctc caggtcctga
    20641 tgaagtcaca acaatcgttt taatttgctg accagctgcg gaggcaaact ggatattcgt
    20701 ccgaatcgta cgatattgtt cagcgattgg tgaagagggg tttgtgagtg tgaccaaact
    20761 gactgcatga gtttgggctg ctttttgttt ctgtgttcgt gccatttttc tttcctccta
    20821 gactcttgag cggcttctgc gtgatggcgc tgttttatcg tttgtagctt ttggttgtgt
    20881 cggagctggt ccttttttac ggataccagc attcaattct ttcgctgaca tttctggtac
    20941 agtccctaag atcgggaagc ccaatgtatc cgtcacatat ttttcatctt ttactgtccg
    21001 gtcgagtaat tcaaataaga aagctagacc tacgcctacc atcattccta acactaagcc
    21061 aatcactaga ttcaatttat tatttggcga aacaggtgtc gtattggcaa ctgcacctga
    21121 gatgatcgag attttatcga cattcatcac gtctttggcg ttctcttgga atacttgtga
    21181 cgttgtatta gcgatttgtt gagccgtcac tgcatttgtg cttgttgctt ggatcgagaa
    21241 catttgtgaa ttttgtgact gattgacgga gatggcatct ttgatttgtc cagcagtcat
    21301 ttttacattg tcttctgttt ctaaacgatc tttgacttca tttagtacta gatcactgac
    21361 gatcatatcc ttatacgtat tgatcatttg caggttcgta ttgacatcat tgacatttgc
    21421 agtatctgat tgcggcagtg tcacgatcaa ttgggcttgt gagctgtatt ttggcgtcaa
    21481 gatgaaaaac gtcacgatgc ctgagatggc caagccagca aacattgaga tgaggattgt
    21541 tatcacgtgt ttgcgtaatg tttcaaaaat ttctttcaag ctgattgtct cttccatttt
    21601 ttctccactt tcctatttat tctctagatt catttgatct tttaattctt ttttcacacg
    21661 atcaagttct tgatcgctga ctttttggta tgagatattg tcttgcatga agccttctcc
    21721 ttgcagctga tcttgcttga tcgtaccaaa tgcatcccga taatctaaag caatcttttt
    21781 catttcatca aagctcatgt ctgttttcat attcgactct agagcattca aaacggattg
    21841 atagttgctt acaccgttca gactcaacac ttttttcgct acaccttcta cgatctttct
    21901 ttgacgttct tgtctgccgt agtcaccatt tggatcttca taacgcatac gagaataaga
    21961 caatgcctga tccccgttca atgtgatttt accgatcgta aaatcatagc catcttgtga
    22021 gaaggtgagg tcattattga cttcgatacc accaactgca tcaacgagtt cttttagacc
    22081 tttcatattg attgaaacat agtgatcgat tgggatgtct acatattttt gtacggtatc
    22141 catcgccatg gcagctccgc cgaatgcgta agcgtggttg atcttgtctt ttgtgtcatg
    22201 tccaacgatg tcgacatacg tatcacgagg aatgctgaca atcgttgtct gtttgtcttc
    22261 aggactgact gtggcaacca tcatcgtatc tgatcttcct tgatcgaccc gacctaaatc
    22321 tcccgtatca atccccatca atagaacaga gaaagattct tgtttgtcca gatcaactgg
    22381 ttttgttcgt tctccttggg ttcgttccac tgattcgtaa gttttttcaa tagaatgtga
    22441 gagatctaga tataattttg ctcctactcc tacgattgct aatactaata cgacaagaat
    22501 tgctaaaata attagaaata tctttttccc tcttgatctt cttcttcgtt ttgccatagt
    22561 ttcctccgtt tgctagcctt gcttgaattc tcgcttatta atagctcgta ttgctatagc
    22621 ccatttgctg agcgactgtt tctgcttttg acgcgtatgc ggctcttatt ttttcattcc
    22681 ggatctgttc gatgagactg agtgcggtca aatattgatc gtaagtagca gtagctgtta
    22741 cttgaccgtc tttcagcatc gtatcgaata gctgatctag ttgcgtgact tgcgcgatct
    22801 gagtatcagc gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa
    22861 agctcatatt ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgacga
    22921 catcgtctac agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg
    22981 tctggatctt cagttttgat tctgcatcct ctaactgcgt taagactgct ttcttttctt
    23041 cttggatcgc agcagcttct ttaggtaatt ctgattcctt gatgtcataa tcatctgccg
    23101 aaagtttgat agaagatagt ttatgtgaca aatttgaaac tgtttcttct gttatttcag
    23161 gatttaggaa aattttttct tttgtactgt acagcttatc tacttctttt gataaagagg
    23221 tcacggtgtc tttttgattg gtgatggcag attctgcgta attgatccgt tgatgatgtt
    23281 gccgctgatt attgaaatgg tatccaaaca agcaaatcac aatagcgcct gtgcctaccg
    23341 ccacagtagc caataacttg acgaacggct tttccataga ttcactccta aatattgatg
    23401 atagataaat aaagatgtga ttcattccca tcagaatga
//
LOCUS       DAYYGS010000043        22878 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000043 DAYYGS010000000
VERSION     DAYYGS010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22878
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.043"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..369
                     /locus_tag="ACHA44_001568"
     CDS             1..369
                     /locus_tag="ACHA44_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFM8904845.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="ACHA44_001569"
     CDS             549..1490
                     /locus_tag="ACHA44_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFM8904846.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="ACHA44_001570"
     CDS             1559..2290
                     /locus_tag="ACHA44_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8904847.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="ACHA44_001571"
     CDS             2434..4614
                     /locus_tag="ACHA44_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFM8904848.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="ACHA44_001572"
     CDS             4634..5116
                     /locus_tag="ACHA44_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HFM8904849.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="ACHA44_001573"
     CDS             5132..5893
                     /locus_tag="ACHA44_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFM8904850.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /gene="murC"
                     /locus_tag="ACHA44_001574"
     CDS             6024..7358
                     /gene="murC"
                     /locus_tag="ACHA44_001574"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFM8904851.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7665..8150
                     /locus_tag="ACHA44_001575"
     CDS             7665..8150
                     /locus_tag="ACHA44_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HFM8904852.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8143..8826
                     /locus_tag="ACHA44_001576"
     CDS             8143..8826
                     /locus_tag="ACHA44_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFM8904853.1"
                     /translation="MNNPSTLMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8952..11597
                     /gene="polA"
                     /locus_tag="ACHA44_001577"
     CDS             8952..11597
                     /gene="polA"
                     /locus_tag="ACHA44_001577"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFM8904854.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA44_001578"
     CDS             11642..12478
                     /gene="mutM"
                     /locus_tag="ACHA44_001578"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFM8904855.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA44_001579"
     CDS             12442..13071
                     /gene="coaE"
                     /locus_tag="ACHA44_001579"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFM8904856.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            13123..13455
                     /locus_tag="ACHA44_001580"
     CDS             13123..13455
                     /locus_tag="ACHA44_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFM8904857.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA44_001581"
     CDS             13767..14258
                     /gene="nrdR"
                     /locus_tag="ACHA44_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFM8904858.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14281..15654
                     /locus_tag="ACHA44_001582"
     CDS             14281..15654
                     /locus_tag="ACHA44_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8904859.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA44_001583"
     CDS             15654..16580
                     /gene="dnaI"
                     /locus_tag="ACHA44_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HFM8904860.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16709..17149
                     /locus_tag="ACHA44_001584"
     CDS             16709..17149
                     /locus_tag="ACHA44_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFM8904861.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17293..17994
                     /locus_tag="ACHA44_001585"
     CDS             17293..17994
                     /locus_tag="ACHA44_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HFM8904862.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            17991..19112
                     /locus_tag="ACHA44_001586"
     CDS             17991..19112
                     /locus_tag="ACHA44_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFM8904863.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA44_001587"
     CDS             19127..20359
                     /gene="pepT"
                     /locus_tag="ACHA44_001587"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFM8904864.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20491..21399
                     /locus_tag="ACHA44_001588"
     CDS             20491..21399
                     /locus_tag="ACHA44_001588"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8904865.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21434..21706
                     /locus_tag="ACHA44_001589"
     CDS             21434..21706
                     /locus_tag="ACHA44_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFM8904866.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            21717..22763
                     /locus_tag="ACHA44_001590"
     CDS             21717..22763
                     /locus_tag="ACHA44_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HFM8904867.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
BASE COUNT         7708 a         3986 c         5015 g         6169 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggcagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaaaa tcttgccaag cccccttttt tgcaacttat
     7441 gataaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaatcaaat caaaaacatg
     7501 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7561 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7621 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7681 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7741 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7801 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7861 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     7921 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     7981 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8041 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8101 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8161 ttaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8221 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8281 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8341 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8401 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8461 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8521 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8581 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8641 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8701 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8761 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8821 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     8881 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     8941 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9001 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9061 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9121 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9181 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9241 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9301 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9361 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9421 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9481 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9541 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9601 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9661 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9721 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9781 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9841 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
     9901 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
     9961 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10021 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10081 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10141 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10201 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10261 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10321 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagaaaaa aaccaagaag
    10381 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10441 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10501 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10561 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10621 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10681 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10741 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10801 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10861 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    10921 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    10981 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11041 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11101 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11161 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11221 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11281 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11341 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11401 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11461 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11521 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11581 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11641 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11701 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11761 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11821 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    11881 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    11941 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12001 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12061 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12121 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12181 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12241 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12301 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12361 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12421 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12481 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12541 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12601 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12661 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12721 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12781 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12841 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    12901 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    12961 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13021 atacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13081 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13141 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13201 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13261 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13321 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13381 gaccacattc ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13441 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13501 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13561 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13621 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13681 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13741 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13801 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13861 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    13921 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    13981 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14041 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14101 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14161 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14221 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14281 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14341 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14401 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14461 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14521 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14581 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14641 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14701 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14761 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14821 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    14881 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    14941 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15001 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15061 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15121 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15181 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15241 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15301 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15361 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15421 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15481 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15541 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15601 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15661 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15721 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15781 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15841 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    15901 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    15961 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16021 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16081 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16141 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16201 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16261 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16321 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16381 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16441 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16501 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16561 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16621 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16681 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16741 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16801 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16861 atttgtcgga acgattgaaa gtatcactca agtagaagca gaagaagaaa cgcttcagtt
    16921 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    16981 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17041 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17101 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17161 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17221 tgggaatttt tttattccta gtctccttat tttatttgga aaatgctata attatctaga
    17281 aggagtgaag ctatgaacca tttagattta tctcagcgct tagccgcagt gggtgacttt
    17341 gtccctgaat ccgctcgttt agcagacatt ggatcagatc acgcttactt acccgttgca
    17401 ttgatattaa gaggaaaaat cgaatatgca gttgctggtg aagtcgttca aggtccttat
    17461 gaatcagcac gtaagcaagt caaaaaagat ggattagaag accgtatcac cgtacgcctt
    17521 gcaaatggtc tagatgctat ccagccacaa gatggtatta cagctgtcac gattgcgggt
    17581 atgggaggtt cactgatccg agatatccta gaagctggca agttgaatca acgcttaagc
    17641 ggacaagaac gtttgatcct tcagccaaac atcggagagc gtacattacg atcttggctg
    17701 caagaaaatc agtataaaat ccaatcagaa aaaatacttg aagaaaataa aaaaatatat
    17761 gaaatcattg tagccgaaaa agaacaaaaa ccagttactt acaatgaaat ggagctgatg
    17821 ttcgggccat ttctgcttga agagaaaagt gagatcttca aatcaaaatg gacaagagag
    17881 cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag cagctaatca agaggcacgt
    17941 caaaaacaaa tcgagcagga aatccaaatg attgaggagg ttttaaagcc atgacaatca
    18001 gcggacgcac tttcatccaa aagtttgaaa gctattgtcc tcagtggcta gctgaagaag
    18061 gagacccagt cggactgcat atcggaacat tggataagcc aatccaacga gtaatgatga
    18121 cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa gaaaaaaatc gatttgctga
    18181 ttgctaaaca tcctccaatc tttcgtcctg taaaacggct ggttactgat cagccacaag
    18241 aaaaaatgta tgcagatttg ttgaaacatg atattgccgt ctatgcagca cacacgaaca
    18301 tggatattat ttgggatgga ctgaatgact ggttttgtga acttctcggt attgaagtgg
    18361 agagttattt agtaaagact catgagattc attataaaaa actggccgtt tatgtacctg
    18421 tggatcatgc acagaaaatg cgtgaagtat tagctgcagc tggtgctggt acacaaggtg
    18481 attataccgg tacaagtttt acctcgattg gacacggtcg atttactccg gaagctggtg
    18541 cacaaccagc gattgggaaa gtagggaaga cagaacaagt tcaagaggca aaagttgagg
    18601 ttattttgcc ggaaactatc gaaaaacaag tgattcaagc catgcggtca gctcatcctt
    18661 atgaggaacc tgcttatgat cttttcgcta tagatgaacc agtcgaaatg tttgggttag
    18721 gacgagtagg tgaattgccg caagaaattt ctatagaggc atttgtagaa caagtaaaag
    18781 aagcgttcca actagatgga ctgagaatag tccagccaaa aaatgcaaaa tcctctgtga
    18841 agcgcattgc gatttgcggt ggaagcggtg aaaaatttta tccgcaagca atcgcccaaa
    18901 gagcagatgt ctatatcacg ggagatatct attatcatac ggctcatgat atgcaaagcg
    18961 ctgggttgat tgcaattgat cccggtcatt atattgaatc gttatgcaag caacgattta
    19021 tcgagaaatt tgaatcttgg aaacaagaag aaaattggga tattaacttt ttcgtttctg
    19081 aaacaaatac aaacccattt caatttaatt aaaaggagag aatactatgt acgaaaatct
    19141 tttgccacgt tttttacgat atgtcaaaac agaaacacgc tccgatgcaa caagcaaaac
    19201 gactccatcg actcagacac aagttgcttt tgcgcaagaa ttgaaaaaag aattagaaga
    19261 gttaggctta gctgatgttt tctataatga agataacgga ttcgttatgg gaacattacc
    19321 aagcaatgtt gatcatgaag tgcgttccat tggatttatt tcccacatgg atacagcaga
    19381 ttttaatgca gtaggagtca atccacaaat cattgaaaac tatgatggcg agtctacgat
    19441 cgtactagat gcggaagaac gctatacatt gaatacaaaa gattttccaa atttgaaaaa
    19501 ttatgaaggc cacacattga tcacaacaga tggcagtaca ttactgggag cagatgacaa
    19561 atcagggatt gcggaaatca tgactgcaat ggagatcttg atcaataatc cttctatccc
    19621 tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa atcggtgtcg gagcggataa
    19681 attcgatgtt gagcaattcg atgtagattt tgcttataca gtcgatggtg gtcctttagg
    19741 tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat attacgatcc aagggaaaaa
    19801 tgtacatcca gggacggcaa aagatacaat gatcaatgct ctacagttag caatcgattt
    19861 tcataatcag cttccagccg atgaagttcc agaaaaaacg gaaggatacg aaggtttctt
    19921 ccatttgatg gcattggatg ggaatcctga agaagcttct atgagttata tcattcgcga
    19981 tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc acacatatcc aagaagagtt
    20041 gaatcagcga tttgatcaag aacgaatcaa agtagatatg tatgatcaat attacaacat
    20101 gaaagaaatc attgaaaaag atatgagcat cgtggaatta gcgaaacaag caatgatcga
    20161 gcttggtatc tctccagtga tcgaaccagt tcgtggagga acagatggtt caaaaatctc
    20221 ttacatgggt attccgacgc caaatctatt tgccggagga gaaaatatgc acggacgatt
    20281 cgaatttgtt tctttacaag taatggaaaa agcaacagac gtgattgtaa aaattgcaga
    20341 attgaacaca aagagttaaa atcgaaaaag aacagcttat gacaaggcgt tcgtcccaat
    20401 gatggaatgt catctgtcta aggctaaagg gcaggacaat ggaattggtt gtcccgccct
    20461 cttttttgaa gagtaaatag gaggtaaaga atggaaaagc tgctagtttt ttataacaaa
    20521 agttcaggga aagatgaggg tgagaaatta gcacgctggt ttcaagatta tgtaaaagaa
    20581 aagcagccag aattatatgt agaactagct gaaactggcc catcgattga tgatgcggaa
    20641 ttagtcaaga aagcagaagc catagaagct gatactttag taattatagg tggagacggc
    20701 acgattcatc atatcgtcca agcgttccaa aaaacattat ctcaatacca tgtcggatta
    20761 cttccaggtg gaactgtcaa caaccttgca cgtgttcttg agattcccct caaaaaagaa
    20821 gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa tagactatgg tcaagtcaat
    20881 gatgaagtca ttatctctac actgacaatc ggaatcttag cagatacagc agtttggatc
    20941 agtcaagaag aaaaacagcg ttacggctcg tggatcttta tcaagaagtt ttttaagtta
    21001 ttgctgaaga agaaaaaata tcatttggat ttagaaacag acgaggagca ttggaaagga
    21061 aaatcccaac tgttgacagt cacgatgtct aactccgtgg gcggttttac aaattttgac
    21121 gactcagcga ccctagatga tggaaaattt catgtaacga tcgtaccttc cttagatatt
    21181 ttccgattta ttttttattt gcctagaatc atgagaggca aattctatac gatccccggc
    21241 attacttatt tcacagccaa acgcatgaaa atccaagcgc aggaaaaatc tgtgggcact
    21301 cgaacggatg gagacacgac agatgatctt ccagttgaat tatctgttgt atcaaaagga
    21361 ttaacagttt attcaccaaa gaaagaagaa aaagcttaga aatagtagtt aacagaaagt
    21421 tggaagaaga gtaatggatg tattgttaca caatcctttg tggttttacc ttttcattgt
    21481 gaatttttac ttattttgtc tgatggggta tgataaacat caggctagaa ctagcggctg
    21541 gcgtgttcct gagaataatt tgatatttgt cagcctgtta ggcggtggtt tgggaggatt
    21601 gatctctcga aaagttttcc atcataaaac tcggaaaaag aagtttacta taggattcgc
    21661 tgcaggtgta cttgttgatt tgattctcat ttattttttc cattaaggag caggagatgt
    21721 cgtctaaagg aacgaaaact aaaatcattc tgaatctgct tttgctgatt gtgatttttg
    21781 ccattattta ttatttaatc aatcaatcat ttgcagatat ctttgccgaa ttgctgtcta
    21841 ccagtgttgt tgttttggca gcggctgtta tattagggac agtttaccaa atcgctgaag
    21901 ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa ttttacaacg aaagatgggt
    21961 tcttgacctc tagctatatt gcgttttacc gaatcgtttc gtttggtaca ggaacgctga
    22021 tctcggagat ttatttttat aacaaaaaag gaatgaagct atcccaggga gcaggggtga
    22081 cagcactcca tatgattatg tacaaactgg cagtgatcac ttatgcatta gctggcttgg
    22141 tcatccaatt ctctcttttt tattctaatg gtccatcaat gatctggttt atcatcgctg
    22201 gtatcctttt gacgatcgtg attgttgcct ttttattggc gatgtccgtc agcttgaatg
    22261 ttcaaatatt ttttgtcagc ttatcgaata agttatttaa aagtgagcgt ttgcggaaat
    22321 gggtggatac atgcaatcag cagatctact ctttaaggga aacggtccaa accattatcc
    22381 aagacaggac tgcgctattg cggatttatc tatggaatat gttaaaactt gtattttggt
    22441 atgtacttcc ttatttgatt ctgattgaaa accatcctaa aatagatttt ctactgacca
    22501 tttcgtttat cagctttgca gtagtgcttt caggggttat tcctacgcct gctggtattg
    22561 gttcctttga atttgtctat ttgttattat tcagaccagt tgttggaaca gttgacgcag
    22621 tttcttcgct tttactgtat cgtttcagtt cctttatctt gccatttctc tatggatttg
    22681 tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga aataatagaa gtaaaaaaag
    22741 agaataaaga aaatcaaggg taggtctttt ataagatcta ccctttttat caaatcaaag
    22801 aaaaacgtta tggataagat aattttacgt ttgtttggct ctttttttat atacatacaa
    22861 tcaattttgt ttttttaa
//
LOCUS       DAYYGS010000044        22801 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000044 DAYYGS010000000
VERSION     DAYYGS010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.044"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            212..1018
                     /gene="proB"
                     /locus_tag="ACHA44_001591"
     CDS             212..1018
                     /gene="proB"
                     /locus_tag="ACHA44_001591"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFM8904868.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1015..2262
                     /locus_tag="ACHA44_001592"
     CDS             1015..2262
                     /locus_tag="ACHA44_001592"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFM8904869.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2338..2682
                     /locus_tag="ACHA44_001593"
     CDS             2338..2682
                     /locus_tag="ACHA44_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904870.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2799..3125)
                     /locus_tag="ACHA44_001594"
     CDS             complement(2799..3125)
                     /locus_tag="ACHA44_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904871.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3740..4942)
                     /locus_tag="ACHA44_001595"
     CDS             complement(3740..4942)
                     /locus_tag="ACHA44_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8904872.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            5059..5574
                     /locus_tag="ACHA44_001596"
     CDS             5059..5574
                     /locus_tag="ACHA44_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8904873.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5773..6219)
                     /locus_tag="ACHA44_001597"
     CDS             complement(5773..6219)
                     /locus_tag="ACHA44_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8904874.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6266..7744)
                     /gene="msr(C)"
                     /locus_tag="ACHA44_001598"
     CDS             complement(6266..7744)
                     /gene="msr(C)"
                     /locus_tag="ACHA44_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8904875.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(8098..8727)
                     /locus_tag="ACHA44_001599"
     CDS             complement(8098..8727)
                     /locus_tag="ACHA44_001599"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8904876.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(8711..9175)
                     /locus_tag="ACHA44_001600"
     CDS             complement(8711..9175)
                     /locus_tag="ACHA44_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8904877.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9662..10930)
                     /locus_tag="ACHA44_001601"
     CDS             complement(9662..10930)
                     /locus_tag="ACHA44_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8904878.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(11161..11898)
                     /locus_tag="ACHA44_001602"
     CDS             complement(11161..11898)
                     /locus_tag="ACHA44_001602"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8904879.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11965..12534)
                     /locus_tag="ACHA44_001603"
     CDS             complement(11965..12534)
                     /locus_tag="ACHA44_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HFM8904880.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12662..13534
                     /locus_tag="ACHA44_001604"
     CDS             12662..13534
                     /locus_tag="ACHA44_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8904881.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13876..14748
                     /locus_tag="ACHA44_001605"
     CDS             13876..14748
                     /locus_tag="ACHA44_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8904882.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14745..16703
                     /gene="nagE"
                     /locus_tag="ACHA44_001606"
     CDS             14745..16703
                     /gene="nagE"
                     /locus_tag="ACHA44_001606"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HFM8904883.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(17035..17886)
                     /locus_tag="ACHA44_001607"
     CDS             complement(17035..17886)
                     /locus_tag="ACHA44_001607"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8904884.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            18009..18476
                     /locus_tag="ACHA44_001608"
     CDS             18009..18476
                     /locus_tag="ACHA44_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFM8904885.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18739..19221)
                     /locus_tag="ACHA44_001609"
     CDS             complement(18739..19221)
                     /locus_tag="ACHA44_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8904886.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(19321..20184)
                     /locus_tag="ACHA44_001610"
     CDS             complement(19321..20184)
                     /locus_tag="ACHA44_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8904887.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20782..21657
                     /locus_tag="ACHA44_001611"
     CDS             20782..21657
                     /locus_tag="ACHA44_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFM8904888.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(21802..21954)
                     /locus_tag="ACHA44_001612"
                     /pseudo
     CDS             complement(21802..21954)
                     /locus_tag="ACHA44_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(22222..>22801)
                     /locus_tag="ACHA44_001613"
     CDS             complement(22222..>22801)
                     /locus_tag="ACHA44_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8904889.1"
                     /translation="NLFFGFGIHQAVISGSLLDPFLIQNMQENMAAYANKEQIPHIIN
                     MAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLSVTPAIFNISEPIIFGLPIV
                     FNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWTTPPVISGFLATAGDWRAAV
                     LQILIIAASVFIYLPFLRIDEHVTTKLSENET"
BASE COUNT         6984 a         4626 c         4073 g         7118 t
ORIGIN      
        1 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
       61 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
      121 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
      181 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
      241 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
      301 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
      361 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
      421 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
      481 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
      541 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
      601 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
      661 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
      721 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
      781 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
      841 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
      901 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
      961 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
     1021 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
     1081 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
     1141 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
     1201 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
     1261 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
     1321 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgatcggcat catttacgaa
     1381 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
     1441 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
     1501 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
     1561 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
     1621 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
     1681 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
     1741 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
     1801 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
     1861 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
     1921 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
     1981 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
     2041 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
     2101 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
     2161 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
     2221 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
     2281 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
     2341 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
     2401 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
     2461 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
     2521 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
     2581 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
     2641 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
     2701 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
     2761 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
     2821 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
     2881 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
     2941 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
     3001 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
     3061 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
     3121 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
     3181 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
     3241 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
     3301 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
     3361 caacctcttg gtaaacggcg ttcaatcagc tgaccctcgg tattagctca aaaaacggta
     3421 aaatatgaga agctattttt ccgttttgtt tctctaatac ttaggataaa acgctgattt
     3481 tacaacctct gttaaacggt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc
     3541 aaaatatgag gaactatttt tccgtttgtt tctctaatac tcaggacaaa acgctgattt
     3601 tacaacctcc tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa
     3661 cgaaaaatat gagaagctat ttttgtttgg ctgttcttat gacttgatgc aggacagctt
     3721 tttcacaacc tctgatttct tattcttctt catttgtagc aaaataatta cggtgataaa
     3781 tgatacaaca tacactgata agaacaatgg cacctgcaaa ataaaacgca tagctttcac
     3841 caatatgttc accaatcaca cctgcaataa atggtgctaa agctccaccg aaaaagcgga
     3901 caaaactata gctggacgaa gcaacgtttc gttgcagacc tggtatttcc atagcaatcg
     3961 tcgtcaacag tgtattgata attccttgga aaaatcctgc taacacaatg ctgatcggta
     4021 caaggactgg catagaagtt ccagctccta ataacagcaa acaaataatg aataagacaa
     4081 atgctgctaa aattgtccga taagtcgtaa acttccgctc aagagccggt gcaacgaaaa
     4141 tcgaagcaat agccagaagc acaccccagc caaaaaatac aaaaccgact tctaattcag
     4201 aatagccaat caataaaaat ggcgcatacg caagtaaagt aaagaatccg aaattatata
     4261 acaatgcgat tagtccaact gaacgaagtt tatgattgct taacgctttg acaccttccc
     4321 aaaggcctac ttttctcttt ggtttctcag tttcaggtaa aagtaccgct agggctataa
     4381 aaccgcaaaa catcaaaaat gctaccccaa aaaatggata acgccatgaa ccactgccca
     4441 agaccccacc gactagaggg ccaacagcca ttccgcagcc aagtgctgct tcatacatca
     4501 tgattgcttt ttctgtgtta ccaactaaaa tactgacgat cgttgctaaa gcagtagaga
     4561 caaataaagc attcccgaca ccccagccgg cacgtaaccc aatcagcatt ccgatattcc
     4621 cagataatcc gccaagacca gcgaatacaa cgatgataaa caatcctgtc atcaatgttt
     4681 ttttcaaacc gatccgacta gataagaaac ctgtaaatag catcaccaca ccagtgacta
     4741 acatgtaact agtaaataac agggtcgttt cagctggcgt agcgtttaac tgtgcagcaa
     4801 tcgtttttaa gattgggtct actagaccca ctcccataaa agcgatcata caggtagctg
     4861 ctacaactag cgctgaactt ttcccctttg tttcttcatt tgtttgttct tgtatatcta
     4921 tttgattctc caatatttcc atccctttct ctctattagc atcatacatg atttatttta
     4981 catgtcaata tgcacatgta aaacgtggaa actttactgt gatataataa agaaaataag
     5041 acgagaggta ggatttttat gaatacttta gcctatatat tattatgcgt gcttttatcc
     5101 aaaccacgtt ctgggtatga attgaagcag ctgatcacaa ttttttggga agcccatcac
     5161 agtcagatct atacaacgct tgccaagtta gaccagcagg gttatgtgac cgttttggac
     5221 aaagacgagc actctcaaaa aaagatctac cacttgactg atgaaggacg tgctcttgta
     5281 gaggaatgga tcaaagagga aactcccgct cctacgcaaa aagatgaatt tctagcaaaa
     5341 atctatgctt tttcgacgct cgataaaaca acagctactg gactgctatt cactagagaa
     5401 caacagctga acaaaatcat gcaaaaaaat caaaaaaaat tgaatgacct ctcgctagac
     5461 aagaaacaag aatttggacg ttatgttgtg atccagcgca gaattatgct ttgtcagcag
     5521 gaactccaat ggtgtcagca agtaagagaa caaatgcagc agttttttga gtaagggata
     5581 aacagaacct ttctagaaat atctattgaa gagcaaatta tcttcaataa aagagcaata
     5641 cagttggaaa agaaaaagcg atccgaacga caggccaaaa cctatcgttc ggatcactat
     5701 ttttctagaa ctgcattcaa gagactatga caaaaagcat gccaaggcct tttttcaaat
     5761 aacagtgttc catcattcat tactttgaat tgcttttagc acacttcttg aaagcgggcc
     5821 aacgatgatc aactgcaacg gtaaagcggc aatcacattc attttccaag cattcatata
     5881 agcagagaat aattgatcag gtattccttg ttccatgatc acaccgaata aagacataca
     5941 tgtcaccatt cctaaaacca tcaagcatga aatagttaag atcatttgga ccttgctttc
     6001 tggatttact ggtagtttaa aggcaatttt tttcgccagc acaccgatga tgaagacatc
     6061 caaaatgaaa gccaccacga atccaggcac tagaccaatc aacaaacttg ataaatgaag
     6121 atcttgatgc agccataaat tatacgcgct catccccaag accatcaaaa aacacatcat
     6181 tgttgtaaaa attattcctt cttttttatt tgtaggcaat ctgtaccact cctttaaatt
     6241 tgtcacgaaa atcatcgtag cacatttaaa aatttctcgt aagtactttt tttattttct
     6301 gcccttttat ttcataaact tcatcagccg tacgctcgac aaagtaagaa tcatgagaag
     6361 taaacaaaat cgttcccgga tatatttgca ttagcttctc taaagcttcg atcgttgcca
     6421 tatcaataaa attagtcggt tcatctaaca gcaacacatt acttggctta gtaaaaagca
     6481 aagcaatcgt cagacgggtc gcttctcccc cacttaatgt acataacggt ttcgtcaaag
     6541 tttcctgagc aaaccctaag tgattcagca aactacggac aagcgattct gaaaactctg
     6601 tttgtctcat gacaaaacga atgatggatt cttcagatgt catttgataa cccttttgct
     6661 gatatacttg aaagcttact ttcggagaga gaaggattcc ttctccttgt ttgcggatat
     6721 gttctaataa ggttgtctta cctgaaccat tttcaccgac aatcgcgatt tttttaccta
     6781 aagaaaactg aaaatcacca tttaccagta atgttctccc acttctttcc aattggacat
     6841 tttgtcccat gatgggatag cggctgtgga gttcaagaga ttttggtact ggaaaacgga
     6901 tttgtttcat ctgctgcggc ttggttgttt ctcctatttt ttggagtctt ctctctaatg
     6961 ttttcgcatt cttttggatg gctttttgta cactgtcttt ttgtttagag gaagacaacc
     7021 gatccagtct gatttgttgc tttttctttt tactggacac tttttccaac ttttccgctt
     7081 gttttttctt ttcttggatc gatttttcca atcgtttttt ctctttcatg atcgactctg
     7141 cttctcgtag ctgagtcttt ttctccaatt ccttttgagt gagataggca ctataattcc
     7201 ccgaaaactc tcggatttct ccgtctttta cttcccagat tttcgatgct agctcatcta
     7261 gaaaatagcg atcatgacta acaaaaacca gcgtaccata ataataacga agctcttcaa
     7321 tcagtcgatc aatacttttt ctatctagat gggttgttgg ctcgtctaga agcatccctt
     7381 gttcataaga agaaagaaga cgtatcaact cgatctttcg ttcttcccct ccgctccatt
     7441 tccgctctga agtttcttcc agtcttaact ggaaatgact ggctaattcc aaatttaggt
     7501 cctttgcttc agcgagatag gttaattgag gcaggtagtt gaaggtgatc tctgtttgga
     7561 ttttcccatg atcgggaaaa agttcaccgg ctattagttt gagcaaagtc gatttaccga
     7621 caccattttc tcctatgatc cctatacgat cttgctgata gacacgaaga gaatcaatag
     7681 atagttctag ctggttgcca aagctgactt gtagatttgt tatatttact gctaaatttt
     7741 ccatatgttt tacactccta tttcatgtaa aacaacaatt atcgatacta aaaaagagca
     7801 gaaaaatctg tttgcaaaaa aactgcatca catcttttcc tactctgaac gaaataacta
     7861 tgactaaatc accaaatctg tcatagcttt cacctattct actgtcgttc gaaaaaatgg
     7921 acatactcca tccatgaaat tgttgtttat acatgtcttt tgaatttatt tagttgttat
     7981 tcaaaagttc gaaacgtaat ttcatcgatg cagtcatttt tccgacctcc ttttttatta
     8041 ggttaccaca cacgattcaa aaaaagcaat agctactgaa tagtaactga aaacctttta
     8101 ttcgaacata gtgatctttt ctggtgctaa acgaacagac tcgtaacctt cttctaccgc
     8161 ttttcctatt gataaaataa taactggaac ataacggtct ttatccaaac caaatgcctc
     8221 tgccaactga tctgcttcga atccaccaat tggatttgtt tcatagccat gatcacgcgc
     8281 aactaacatg aattgcatag ccgctaagct agaatcgact ttgactacat cgttcatttc
     8341 ttctcttgaa aaactcttgt aataaggaat gatcgcagct aattgttgat ctctgacttc
     8401 ttttggcatc ttccctgatt cgtaagcttg gttgtagatt tcttcgccat attcgtaaca
     8461 ttgcatatcg ccaaaaatca tgaccattgc agaagaagta tcattttgtc tcgtattgaa
     8521 acggatcaaa ggacgcaaga tttcttttgc ttcctcgctt tcagcgacca caaagcgcca
     8581 tggtttcata ttgacagaag aaggtgcagt cgtcgcttct ttgatcattt ccagcatttc
     8641 ttcgtgtggg attttgtatg tttcatcata tacgcgaacg gatcttctac cagtcataat
     8701 tgttgaaaaa tcattttttt gttccattat tattaacctc tttcgtttct gttgttatat
     8761 tgcaaagttg ggtattcaat ttatctaaaa gtgttagaaa ttgtttcatt tctacatcgc
     8821 tgaaagccgg ggatagcacg gaacctgtat ctttatggtc catttcacaa tgcttgattt
     8881 tcttctgggc tttttctgtt agcgctacat acacttcccg gttgttctga ggatttctct
     8941 gacgcgaaac atagccattt gcttccaata ccttcagatg acgagtaact gctccttgat
     9001 caatctgcaa cttttcttgc aaactgcttt gtagcaatgg attttcttct ttcagtctca
     9061 tcatgatctc gtatctagta agacttatac cgatattttc ttcaaagatc tggacgattt
     9121 tttgatcggc taatttgatt tgatataaaa ggtgactaat ctcttctaaa gacataattt
     9181 cctcctttct aataattgac tcgtcaacga ttgacttgtc aataatagaa taaaaaaaga
     9241 aaaaagtcaa agaatcagct gtgtaataaa aaaaacgctc tcaatagaaa agtatagaga
     9301 aaagaaattt tttgtagaat gaacggaaaa aatgataaag aaagcacaac gctaaaacca
     9361 agattattga ttcttatcaa actatagaag atagctcttc tgcagtagat aattttacac
     9421 cgccaaaaat ttccgaaatc gccttttgca agaatcccgt cgaatattag aaattggatc
     9481 agtatgctta aacagtaata aaaaagttat gacaagatgt tgtcataacc ttttttgagt
     9541 aaacggtgtt caaaaacttg catctgcggt aataagttca accaaacaaa aaacatgaaa
     9601 atctgttttt gtttggctgt tcttactact tgatgcaggg cagctttttc actacctctt
     9661 tttaataacc ataaaatcct attccttcat atctccagcc catcggtgtt agccaatctc
     9721 tttccttagc actcaaggta tagtgatggg taccagtttt ttcatttgga ttaaatagtc
     9781 gataaattgg cttggaacca ccagaataaa aacttagacc ttcataacgc caaccgattt
     9841 ttgttaaaga gttgatctca gattgtgcca ttgtgtaatg atggtctcca ctgtaaggat
     9901 tgtataaacg gtagacaggt tgtccgctgt ttggtgcttg ccaagcaatc ccttcatatt
     9961 cccagcctac agttggcaga taattttttt ctccggcatt caatgtatat aaatgttctc
    10021 ctgtatgacg gttatacaga cgatacatcg gagaagttga agttggtgct gtatcatact
    10081 gtgtcagatt ataggtttcg attacattgt tcaatgtaga accataatta ggcgcagtgg
    10141 cataacgacc tgttagccaa gccgtagcat ctttgtaaga gttcgtattg cttttccatg
    10201 caccggaata gtagtaaacg cctggctgaa aagaagtcgt cttcaatacc gttacgttat
    10261 cttgcaatga ttctttgtac gaaggatatt ttctaaaagc ggcattcacc gtaatccatt
    10321 gcccattcac atattccgaa gtaggcatcg taacagattg gccattatag cttcctttaa
    10381 tcccaaatag attgtaatta ggggcagaag ccaaagtact gttcccccag ccactttcaa
    10441 cgatggcttg agcaatcatg actgatgcat ataagtcatt ggcacttgcc aaactttgtg
    10501 cagttgaggc aatactatca atgaacgctt gttgagaatt agcagatgag cgagcataaa
    10561 aagcatagcc agtatagttt tcgaattcag acaaatctgc ttgatgggta tgtgtcaatg
    10621 tttgcccatc tgtcaattgg ttcgtgattt cttctgcatt ctctggattg ttctgagctt
    10681 ctgctgatgt actgtcttcg gtttgtgttt gttctgttgt ttcagttgtt ggttcttcgg
    10741 ttgaaattgt attttctgag acagtagctg actgcaaagt actttctgta tcgttttcaa
    10801 ttcctgtttc tgaagaactc gatgtctgag ttgcgacagt ttctgtcgat tcttcagcta
    10861 gcactgttgt gttggcaact gctagatttc ctaaaagaat aacgctaaat aataaattct
    10921 ttgctttcat cttttttctc ctttgaatta tgaaactttt cctaaataac agtgtaacta
    10981 aaaaagtaaa agcaagtcaa tgtccatatt taaataacat ttgtttcttt ttaataaatt
    11041 tgctttttct gcataaaaag acgtaaatga agaatattct tctccattca cgccttaaat
    11101 tgatggtcgt ttgaataact atttaacaag taattctctc tggatattat ccgttatgga
    11161 ttatacttcc tgtgtcgtcg gacggataat catgttgctg acagacatcc ggtctggcgt
    11221 ttctatcgca aatgctacag cagaagctat atcttcagat gttaatcccc attctttttg
    11281 cgcttcatga agttcaagag cagccttttt gtcgctgatg gtttgatata attcagtttg
    11341 aacagctcct ggagagatga tcgtagattt gatgttattt tgccgttgct cttgacgtaa
    11401 tccttccatg attgcacgga cagcaaattt tgttccgcaa taaacggcgg aatcaggata
    11461 aacaacatgt cccgccacag aatcagttga aatgatatgc ccagattttt gtttttccat
    11521 gattggaagt acggcggaaa ttccatttaa cacacccatg atgttgatat caagcatatt
    11581 ttgccattca tcacggtgac cttcaactaa tggagcggtt ggcatgattc ctgcattatt
    11641 gtacaataca tcgattcgac cgaacttttc catcgctaaa tcgataactg cttggacttg
    11701 tgcaaaatct cttacatctg ctgtttgaat atatagctca gcttctggta aagattcttt
    11761 tattgctttt aggcgatctt ctcggcgtgc agctataact agtttagccc ctttttctgc
    11821 taatagcttc gttgttgctt caccgattcc gcttgaagcg cccatgatga caatcacttt
    11881 ttctgataaa tcattcattc tattctcctc ctgttttgaa aaatctcttg cttatactca
    11941 ctatctctta tttcatctta ctgttcaaat aatccgttcg acaggtttga cgattttggc
    12001 agggattcct gcaacgatgg tattgtctgg gacatctttc gtaacagttg cattcgctgc
    12061 aacaatggca ttctcgccaa tcgttacgcc tggcagaata gttgctcctg ctccgatcca
    12121 agcatttttc ttgatgtgga ttggctttac tgacaagcct cttcgttctt ttggatcggt
    12181 tagatggttc accgtgatga ggttgacacg aggaccaatc aatactgaat cttctaaaca
    12241 gatgccacct aagtcaacga acatcacttg acgattgata aagacatttt tccctatact
    12301 aatatgtttg ccgaaatctg tataaaaagg cagcagaaca gtcaccgtat catctacttt
    12361 gtcactaatg atattttcta gccgtttgcg gatatctgct ggttcatggt acccagtatt
    12421 cagcgctgct aaaggctctt cgttttccgc ttggatctga tggatcactt caaagagcgg
    12481 atccgttact cgaatctctt tattgacgat ttgtttttcg atgaggcttg tcattttgct
    12541 ccctcctttt atccttactt tataacggac ggcttatgat gtctaatact tataaaacat
    12601 acttaactat tccttttaac tatacttagt ggtatgcttt tttaaaaaca ggagggaaaa
    12661 aatggatatt cgtatactga cgtatttttt agcagtagct agagaaaaaa atatcagccg
    12721 agcagcaaaa tctctgcata tcacgcaacc tactctctca aaacaactga aagatttaga
    12781 agaagaactt ggtgtgacac ttttcactcg aggctcccga gagatacagt tgacggattc
    12841 tggtcgatat ctttattcaa aaggaaaaga aatcttgtcg ttggtagaaa aaacggcgaa
    12901 caatttaaca aatgacgcaa tcatctctgg agaaatctac attggcggag gcgaaactaa
    12961 agcaatctct ttcatttctg atagccttca ttcgttgatc aaggcgcatc ctgatatcca
    13021 gtttcattta tatagcggga atgcagatga tatcatagac aaactggata gcggattact
    13081 tgattttgga ctcgtaatcg aaccgacaaa caaacaaaaa taccagtcat ttcgactgcc
    13141 agcccaagat cgttggggac ttttgataca tcggaatcat cctttagcaa agcaaacagt
    13201 catccatccg caggaactga aaactgttcc tttgatcatt tcccggcaaa cgtcagtgga
    13261 cagtcaacta gctgaatggc taggtttcag catagatcag ttatcgatcg tcggaacata
    13321 taacttattg tacaatgctt ctttgatggt cgataacggg acttttggtg cattagctct
    13381 agatgggatc atcaatactt atgggacgga aaaagtattc atcccactcg atccgccatt
    13441 acaagcttca ctgaatctca tttggaaaaa agaccagcct ctttctcctg ctgctcgtaa
    13501 atttttagat gtcctaacgg agaaatacag ttaagtagag tttttttcct tcgctttcag
    13561 ctttttaaca acgaataagt taagcaaaag atccaatgat taaataacat aagtttctta
    13621 atttgttcct tccttttatt tctgataatt ccagtaaatt actataatta taaaaaaaga
    13681 attgacaaaa cataactgtc tatataaaat tgattacgtt aacaataatt cgtttcatgt
    13741 gactagatca tactaggcat gaaaaatctc tgaggaacat ggtttcccta ggaggttttt
    13801 cgtgtctatt tttgtttggc cgcaatcaac tggtctagca aacgtgaaaa ccaaatcttc
    13861 aaaaagggtg cgcgcatgaa aatcaaaaag atctttaatc aaaatgcagt attggtagat
    13921 gacggtggac aggaaaaagt agctattgga aaagggattg gatttgacag aaaaagaaat
    13981 gatttgattt ttgatcgtga tgtcgaacgg atatttgtca tggaaccaga aggtcaaatc
    14041 aagttgcaaa atttacttaa tcagattgat gaacgctttt tatttgcagc agaaacgatc
    14101 attgatcatg cggaaactgt tttgatggaa aagttgaatg aacatgtatt gatcgctttg
    14161 accgaccaca ttgctttttc tgcggagaac attaataacg gtatcgtcat ccgcaacaaa
    14221 cttttaagag aaatcgaagt gctttacagt gatgaattca gtattgctca atgggcagta
    14281 gagtatctga caaaagagct tgggattcca tatacttatg acgaagcagg atatatcgca
    14341 atccatattc acagcggacg aagcggtcgg aacaataacc atcgcagtat ccgtgaagtg
    14401 acgattgtat ctgacatcat ccatttagtg gaatcagaat tggaaacaga tatccattca
    14461 gaaccatttt cactgaatta ttcaagactg gtgaatcatt tacgactgct tctacaacga
    14521 acccatgcgc aacaatatgc tgttttagac acggaaatcg tagaaatggt caaacgaaaa
    14581 tacccagaaa gctataagat ttctaaaaaa atccgtgtgt tgctgaccaa agaataccag
    14641 ctcgcgatca cgaaagaaga actgggttac ttggccatcc atatcgagcg tttaagaagc
    14701 gcaatcgtac aaacaaatgt taacaatcat gaggaggaaa aaaattgaaa gcatatctac
    14761 aacgaatggg acgttccctt caacttccag ttgcggtctt acctgctgct gctttactag
    14821 tagggatcgg aaactggtgg gcatcttata gtaacgacat tgtcgctcat tttttacaag
    14881 ctggcggtaa cgcagtttta ggtcaattac ctttgctgtt tgccgttggg ttagcactag
    14941 ggatgtcagt ggacaaatca ggtgctgctg ccttggcagg tttggtcgca tttgaagttc
    15001 cagtaaatgt tttaaaaact gattcagtag ctactttatt gaatatcaaa ccagaatcag
    15061 ttgatccatc ttttgctcag atcagtaacg tatttattgg tattattgcc ggattgattg
    15121 cggcagctct ttataatcgt ttccacgaag caaaattacc gatggcttta tctttcttta
    15181 gcggcaagcg tctagtgcca atcttggctg cctttgtcat gttgctgatc agtgcagtgc
    15241 tactgctcgt atggcctccg gtttactctg ctttagtttc ttttgctaaa ttcattttga
    15301 gtttaggctg gggtggtgca ggtctttatg gcttcttcaa ccgtttactg atccctacag
    15361 gattgcatca agcaatgaac tccgtgttct ggtttgacgt agcaggaatc aatgatattg
    15421 gtaaattttt ggcttctgaa ggaacaaaag gcgtaacagg aatgtatcaa gctggatttt
    15481 tcccaatcat gatgttcggg cttccagcag gagcatatgc tatttacaga aatgcacgtc
    15541 cagaaaacaa agcaaaaaca gcttccttga tgttagcagc agcttttgct tcattcttta
    15601 ctggtgtaac agaaccactt gaattctcat ttatgttcgt tgcttggcca ttatatgtct
    15661 tgcatgcaat tttcactggg atttctttag cagtgagtgc attcttccat tggacagcag
    15721 gatttgcctt tagtgccgga tttgtcgatt acttcctatc tttgaaaaat ccgattgcga
    15781 atcagccatt gatgttgatc gtccaaggat tagtaacagc agtgatttac tactttggct
    15841 ttaactttgc tatcaaaaaa ttccatctga tgactcctgg ccgcgaggaa gcagatcttt
    15901 cagatgacga tactgcaacg actaattcag acaataaata tgcggcacaa gcaagcaaaa
    15961 tctacgctgc attaggcgga gccgacaatg taacttcgat cgataactgt actacacgcc
    16021 ttcgtttgca agtcaaagat acaggcctga tcgatcaaaa taaaatcaaa gccactggcg
    16081 tacctggtat gaagatcatc gacggaaaaa atgcgcaagt catcgtaggt acagaagtac
    16141 aattcgttgc agatgagatg gcaaaactac atggtggtgc tgcaacacga cctgcacaaa
    16201 caaacaccgt ggttaaaaca gaaacatttg ccggtggaac gatcgaacaa gatatttacg
    16261 ctatcgcaaa tgggaaactg attccaatca ctgaagtttc agatgatgta ttcgcggaaa
    16321 aaatgatggg tgacggctat gctgtattac cagaaaatgg agaaatcttc tctccaatcg
    16381 ctggtacgat cacaaacatt ttcccaacga aacatgctgt cggaatccaa acagatgctg
    16441 gtatcgaagt cttgcttcat atgggcatca acactgttga cttaaaaggc gaaccattca
    16501 ctttatatgt aaaagaaggt caaaaagtcg caagaggtca attgattgct ttagttgatc
    16561 ttgccgctat tcaatctgca ggcaaaaaca cggatatggt cgttgtattc acaaatggcg
    16621 acaaagtcca aagcttagaa atcgaacccg cacgtgatgt aaaagcaaat gataaaatcg
    16681 gctctgtttc caacaaagcc tgagccatcg tcttccccag acttttttat cgataaacaa
    16741 acaggagctg agacaaaaat cttgtctcag ctcctttttc cgaataaaaa cggcgttctc
    16801 ctgccaactc cgcggtaata agtccagcca aacgtaacat atgaagagct attttttatt
    16861 tgactcttct tattacttga tgcagaacag cttttttaca acctctgata aacggtgttc
    16921 aaaaaccagt ttatcggtat gaagtcaaat ttatgcaaaa atgtggaaag ccattttcac
    16981 aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta tacattattc
    17041 ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc ccaatacttt
    17101 ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt ggatcgctaa
    17161 aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga caaacgctgc
    17221 atcacttgct tcaataattt ctaacttttt acctgtcgca cgctctaatg ccttggctaa
    17281 ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt cactttctac
    17341 tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg tttttccatt
    17401 tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag gtaattcatt
    17461 ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct taatcatttt
    17521 ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat ctgcaaaact
    17581 tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat tcttatgttc
    17641 ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc ctttcaacgc
    17701 ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc cagcttcttt
    17761 cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt gtggaaccat
    17821 cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg ttactaaata
    17881 atgcatattt gccactccct attcttttta tttgttaaga tttacagctg tacatttata
    17941 aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa atgaaaggat
    18001 gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt gcctatattg
    18061 aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt atcgaaacaa
    18121 atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg atctttacaa
    18181 aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct ttactagatg
    18241 tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa acaaatccaa
    18301 attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct cggctgcaga
    18361 aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat aaaatagccg
    18421 ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt ttataattct
    18481 ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt tattctccgg
    18541 gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa agcgctgaag
    18601 tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt tctgaaaaat
    18661 gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca aagcgctact
    18721 ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt ctcccatata
    18781 tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat aggcgtaatc
    18841 ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc ttaattcttc
    18901 aggcgattct gctaaagcga ttacaacaag atacgttcct tttgggaatg ggatgattcg
    18961 ggtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca ttttcccgtt
    19021 atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca atgcatctat
    19081 tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca aaccgacaaa
    19141 atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat ctggtttttc
    19201 ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt catcttacat
    19261 aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt tattttttaa
    19321 ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga aatcctctcc
    19381 ccattttgta aaagagatct cgggagcttc gactaactca taatctgaag aaggaagcca
    19441 ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc ctttattagg
    19501 aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt acggattttc
    19561 ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca agcgggctcc
    19621 atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta acttatgcat
    19681 ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt gattgactcc
    19741 ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt ctttcttttc
    19801 gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca agcgtggaaa
    19861 agatttttgt atgccgtatc tgctgatttc tgaaggcaga tatcctgacc aatcacgaaa
    19921 agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt ctgtcactct
    19981 tgatccatct aggagatcgc gattagccaa agccaatcta cggtttttga tatagtccat
    20041 tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag agattcctgt
    20101 attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga tgtaatcgat
    20161 caattggttc agatctttaa gcatatttat cttcctcctt gcttgccttt ttatcatatt
    20221 atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg tagaaaatgt
    20281 tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg gtattatctg
    20341 cctatctatg ttttaccttt agaaaaataa attttgggga agacacattc taagtaaaca
    20401 aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac tgtacaaagc
    20461 aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc gatacatcat
    20521 acctctttta tcaattggaa caattattta tttctaacaa aatcacttca tttttgtcag
    20581 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
    20641 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
    20701 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
    20761 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
    20821 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
    20881 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
    20941 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
    21001 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
    21061 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
    21121 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
    21181 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
    21241 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
    21301 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
    21361 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
    21421 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
    21481 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
    21541 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
    21601 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
    21661 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
    21721 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
    21781 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
    21841 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
    21901 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
    21961 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
    22021 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
    22081 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
    22141 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
    22201 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
    22261 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
    22321 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
    22381 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
    22441 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
    22501 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
    22561 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
    22621 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
    22681 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
    22741 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
    22801 a
//
LOCUS       DAYYGS010000045        22749 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000045 DAYYGS010000000
VERSION     DAYYGS010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.045"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            130..3408
                     /locus_tag="ACHA44_001614"
     CDS             130..3408
                     /locus_tag="ACHA44_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HFM8904890.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3949..5856)
                     /locus_tag="ACHA44_001615"
     CDS             complement(3949..5856)
                     /locus_tag="ACHA44_001615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HFM8904891.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5986..6861
                     /locus_tag="ACHA44_001616"
     CDS             5986..6861
                     /locus_tag="ACHA44_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HFM8904892.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6941..7915
                     /locus_tag="ACHA44_001617"
     CDS             6941..7915
                     /locus_tag="ACHA44_001617"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFM8904893.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8095..9240
                     /locus_tag="ACHA44_001618"
     CDS             8095..9240
                     /locus_tag="ACHA44_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8904894.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9377..9721
                     /locus_tag="ACHA44_001619"
     CDS             9377..9721
                     /locus_tag="ACHA44_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HFM8904895.1"
                     /translation="MAFETTATLLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            9992..11278
                     /gene="efmA"
                     /locus_tag="ACHA44_001620"
     CDS             9992..11278
                     /gene="efmA"
                     /locus_tag="ACHA44_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HFM8904896.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11416..12015)
                     /locus_tag="ACHA44_001621"
     CDS             complement(11416..12015)
                     /locus_tag="ACHA44_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8904897.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12419..13540
                     /gene="mnmA"
                     /locus_tag="ACHA44_001622"
     CDS             12419..13540
                     /gene="mnmA"
                     /locus_tag="ACHA44_001622"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFM8904898.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRTITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13786..15207
                     /gene="arcD"
                     /locus_tag="ACHA44_001623"
     CDS             13786..15207
                     /gene="arcD"
                     /locus_tag="ACHA44_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043858 - arginine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HFM8904899.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15221..16339
                     /locus_tag="ACHA44_001624"
     CDS             15221..16339
                     /locus_tag="ACHA44_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HFM8904900.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16399..17691)
                     /locus_tag="ACHA44_001625"
     CDS             complement(16399..17691)
                     /locus_tag="ACHA44_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8904901.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17841..18401
                     /locus_tag="ACHA44_001626"
     CDS             17841..18401
                     /locus_tag="ACHA44_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8904902.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18530..18907
                     /locus_tag="ACHA44_001627"
     CDS             18530..18907
                     /locus_tag="ACHA44_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HFM8904903.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18840..20912
                     /locus_tag="ACHA44_001628"
     CDS             18840..20912
                     /locus_tag="ACHA44_001628"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8904904.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21123..22070)
                     /locus_tag="ACHA44_001629"
     CDS             complement(21123..22070)
                     /locus_tag="ACHA44_001629"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFM8904905.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22316..>22749
                     /locus_tag="ACHA44_001630"
     CDS             22316..>22749
                     /locus_tag="ACHA44_001630"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFM8904906.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQ"
BASE COUNT         7369 a         4148 c         4814 g         6418 t
ORIGIN      
        1 aacaacttaa gttatatttt ttctttttat aaaatacact tattataatt ttgtttttta
       61 ttgacagtaa aaataaatta ggtaatactt taagtaaacg ctttctaatt tgttacggga
      121 ggaatacact tgttaaaaaa acgtataaaa tacagtgcgc taatgatgaa tctccttatg
      181 ctcagtaccc cggttttaat ttctgtcgtt gaagttgctg ctaacgaaca acagtctgtg
      241 aacgaagaaa atgcacacgt gaacattgaa gggatgtcaa caatggagga aataccaaga
      301 actagtgaag atatggatac ggaagaagga aatgatccta tgatgaaaga atcagaaaat
      361 gaaaagacaa aaaagcaatc tgaagaagca aatgaagagt ggaaaggggt tgttttcgga
      421 gaaagcacaa gtgctagtag gtatgccatt gaaccattag aagacggcgt gcggctctct
      481 tccacgaata acggcggaaa attccagtca agcggatcag atggaatgac ctattattat
      541 caagcaatcc ccaaagacat caactttagg atgcgggcga cgatcgaaat agaatcttgg
      601 acctatacga atgcccaaga aggatttgct ttaatggtaa gagacgcagt accaaaaaat
      661 catttgtttg ggcaggcatt ttattcaaat tcatttgcca tactaggcag tcggatcgaa
      721 tatgtctggg actcggatag acaagaagtg gtcaacacga gcggcagcaa atatacgatg
      781 cgacttggat tggggacacg ggcaatcaca ggaatatcat ctgaagatcc gcaagctgcg
      841 ccagctcctg ggagtgtctc agtggaaaca acacctttag agacttcagc agggttcttg
      901 gaaaaagaaa caggttctta taatatattc ggaaacggtc atgggaatct gtttccggcc
      961 acgaactcga ttactaaact ggacgtagag atgaaaaaaa cgaatactgg tttagaggca
     1021 tattattacg atcctgaaac aggagaagaa atggcttctg acatcatgta tgactgggaa
     1081 aaattatacg cttcagatga atcggttatt tatgcaggat ttgctgcagc aaggaatatg
     1141 acgatcaagg tagaaggaat cgaagttgct tactcagatc ctgcgacaga tcctcctggg
     1201 caagaaagac ctactcgctt gattgatccg atttacactg tgacctctag taatcattca
     1261 ggaaaccaag atcatactat gtcctttaga gcaaatgctg acggattatt gacaattaaa
     1321 aataaagcaa caggagaggt gcttaagaac gcaaaaaact tggctatcac tgcgaatcgg
     1381 gaatttgatt atcagacaaa actgatggaa ggaacgaacg aatttacttt cagttttacg
     1441 ccagataaaa actatccgcc agaagaatac gtagagatga ctagttatga aacaaaagag
     1501 attcttcata cagtggacta ccgtaaacct aaatattcga cagtttatgt aacaccagaa
     1561 gggtcagatg caggaaacgg aagcagacaa aatccattat ctctgacaca agcattgaga
     1621 tacgcatatg ctgggcagac aatcgtcatg gcgcctggaa cttattcctt cgaaagagga
     1681 ttgacgattc caaaaggcgt taacgggaca agagaaaacc cgattcagtt gattgcagag
     1741 aaaaatccag aaaacaagcg tcctgtactt gattttaaag gaactggaaa tgggatgaca
     1801 ctgttcagtc actactgggg tttacgaggg tttgacatta caaatagtgc agctatgcaa
     1861 aaaggattac agatttctgg aaatcataat ttgatcgaag agattcatgc ttatcggaat
     1921 ggaaatacag gaatccaaat cagcggtagc tcaaatgatc ctttcgaagc atggcccgcg
     1981 cataacttag tcaagaactg tacatctttt gaaaatgccg atccaggatt tgaagatgcc
     2041 gatggatttg cggccaaact gacaatagga gaaggaaatg tatttgacgg ttgtatcgcg
     2101 tatcataatg cagatgacgg ttgggacctt tttgctaaaa atgaaacagg ttcgatcgga
     2161 aaagtaatca tcaaaaactc agttgcttat cgaaacggct gggtaccagg aattgaggga
     2221 gaaggtaacg gtaatggatt caaaatgggc ggttcttctt taacgggccc tcatgaattg
     2281 atcaatagtt tgtctttcga aaatcttgcg aaaggaatcg attccaatag cggaacagat
     2341 attatcgtca atagcagtac aagttttaat aacggttcat ataatgtggc tctttatacc
     2401 tcttcagcag gaaacacaga ctaccatgct tctggtatct tatctttccg aacagagaat
     2461 cttgagatga gagagcagat ccgcccattg aatcaaaacg aagatcaaat ctaccactcg
     2521 ttgaactact attggaatga acgagagaaa caatcagaaa atactcttgg acataccata
     2581 tcaaaagatt ggatcgagtc tctaaataca tgttcgaaag aaggtcagca gcctgttacg
     2641 cgctatgaaa atggaagtat ccatgttcat ggactgatgc agattaaacc ggggaatcgt
     2701 caaacagaaa aggaaagagt ggatggtctt ccattatctg taggtgctat ttttgacgga
     2761 gaaacctcac cgtcttcgga atatccagag tatgcaatcg tacaagaacc aaatcagaca
     2821 aaaccaaatg aacaaaataa tccgattatt atacaagaag caaatggtgt ggcggagaat
     2881 gcagaaggaa atatcattga actgaagcct tttacagttt ttgtgtcaac tttcacacca
     2941 aatcaaggaa cgcctgtctt tgaagatatg cgttatttcc cagaaaagat ccaaatcgtt
     3001 gaaacacagt caagtgctca attttctaat cataccgcta ttttttcagc accttcaaaa
     3061 ggagaatata cgctagaagt cacttatcgt tcggaaatat ttgatgggga aaaatggtct
     3121 gctcatacag aatctgttac ccagcaaaaa gcgatcactg tgcgagaaac tgccgatgtg
     3181 cccccgggaa atgacaaacc agaaagagaa aatccaggaa atgaatcacc aaatggaaaa
     3241 ccaaatggaa aaccttctca tccggatagg acagcaaaag aaagtgaaaa aaatggcact
     3301 caaggactcc caaaaacggg agaatttaat aacccgttgc tagcattagc tggaggaatt
     3361 ttgttaatcg gtgtagttgt ttatgtcatg aaacagcgaa aaaaataagt ggaatttctc
     3421 cttttatgat gtaaaacaat tgactgagga aagtaaaaat agaaggaaat gagaccaata
     3481 gatgtctcac ttccttctat ctttttatag atgaggttct ccttgctgct tttgattggc
     3541 tgttcttatt acttgatgca ggacagcttt ttcacaatct ctccactaaa cggtgttcaa
     3601 tcagctgacc ctcggtatta gctcaacaaa cggcaaaata tgaggaacta tttttccgtt
     3661 tttttctcta atactcaggt caggacgctg atttcacaac ctctcgataa acggtgttca
     3721 aaagccagtt tctcggtagt aagtcaaatt tatgcaaaaa tgtggaaagc cattttcaca
     3781 aaatttttct tactacttga aactaagcag ctttttcaca acctctaata catggtattc
     3841 cagaagtaac ttcttcgaaa aataagccga aatgtcacaa aaatttgaga agcgattttc
     3901 ccaaattcct tcttatttct tgaagctgac cacttctggc acaacctctt accagattcg
     3961 cactcttttt tctggcgcta gatacatagc gtcttgttcc gtgataccaa atgtttcata
     4021 gaattcgtca aggttttttg gttgcacgtt tgctcggagt ttagcagggg catggacatc
     4081 gatttgcagt aaaagttgtt ggtattcttt ttttgctttt gtacgccaaa tcgttgccca
     4141 attgacaaag aattcctcga tagaaagatc tgattccgtt tttgctgctt ccaatgcaca
     4201 gcttagaccg cctgcatcag caatattttc cgaaacggtt aattttccgt ttacttttcc
     4261 gtcagcaaaa ggcagaccat cgaattcttc gatcattgct tgtgctttgt tttgaaattg
     4321 tgtaagatct tcttctgtcc accagttgtt caagttgcca tattcgtcaa ataaagcgcc
     4381 gttattatca aatgcatggg aaatctcatg agcgataaca gctccgattc caccataatt
     4441 agcagagctt gattgttcta gactgtaaaa tggtgcttga aggatcgctg ccggaaagac
     4501 gatcacattc cgaaatggat gataataagc attgaccgtg tcagcactca tttcccattc
     4561 tgaacgatcc actggtttgt tccatttgct gaaacgatct tttaatgtga gtttgctgaa
     4621 aacaagagta tttgaaagca aagtaccacc ctgatctgca ggtactgttt tgaactggtc
     4681 gaataatgct gggatcttat cggggtagcc tacttggata cctaagttat ttaattttat
     4741 gacggctttt tcttttgttg catccccaag ccagtcattt tcttctagac gttttttata
     4801 aacggcgatc atttttttca ccatttgttc tacgtctttt ttagcagctt cgccaaaata
     4861 tttttttccg tagtaatcgc caactacttg atcgaattgt ccagaggcaa ggtaatacgc
     4921 tgcttttttc tgaggcattg cttcgtctgt tccagacaat acgcgagaat agattccgct
     4981 gacttggcgg aattcttctg atagataacc actaagagaa cgtactgttt taatgatcat
     5041 ccagcttttg aataacggaa aatgagcttc tgtcaggatg tcattcaaat gatcgaagta
     5101 cactggatca gttacgatca ctttatctgg agttgtccca atcaaacccg tgacaagttt
     5161 tttcaagtcc agtttatctg tgtatttaat aaatgtatca aaagattgcg gattatacat
     5221 tttgctgtaa tctgcgtttt cttctgcact ttttacatga ggagcaatca atttatcaaa
     5281 ttgcactgct tgttccgtga ttgcttctgc ctgacttttt tctttgccgg caagctctaa
     5341 taactgaaca gccatatcaa agaaaacgcc tattagttgt gcaccgttgg gatggtctga
     5401 tgcataatac gtcttatctg gaaggaataa agaaggcgga taagcaaaga aggcgttcgt
     5461 ttgcgcattc ttcatatctg catctacatc cagattaaat ggcaatggta atgtatccaa
     5521 cagccattct ggaaattgct tattcaattc gtcgaaagac tgcaatgatt caactttttc
     5581 gattataggt tgaagcggtt gagcatgcaa ctcatttctt cggtcataat cgtttgccag
     5641 ttgataatat gcaaggaaat gtttcatttt ttctgaagac actttttctg gatgggcaag
     5701 cagatcgtct gtttctccca tcaaaagttg gtcgatcccg tcaactaaat cttggaaacc
     5761 gcctgtagct ggtttatctg cagggatttt tgctgttttt tgccattctt cattgactgc
     5821 ttcatagaaa tcttgtttca atcgttcttt gttcattcat cgatcgctcc ttttttgttt
     5881 cgtgtgccca ttatcttaca aaatgaagga attaacaaat agaacataaa aaattgaaaa
     5941 ttcgaacaat caaactgatt tgtttttttc aatccttcac ttataatgaa aaaaaggagg
     6001 cgatcccaac tgaatcaagt agtggataaa cctttatatt tcaaagtaga taaaaaaatt
     6061 aaaaaattag ccagcactca gcagctgcag tcaagaaaaa gtaaattgct ttttttagct
     6121 ctggtttttg aagatcaaag ttatgtgatt atcgatcaat caggacatcc ggtagaatat
     6181 tctccagcaa aatatactta tcaagagggt ctttcttgta aaaaatggaa gctgatcaat
     6241 gaagagccaa tcgagttatc tagatggatc aacagaaaag aagatattcc cgttttgatc
     6301 gaagaaaaaa ggtcaggaaa ggaactcgca aattgctggg tgggattgcc agaagaacgt
     6361 tttttgaggt ataagaaatg ggctaccccc tcaggctatc tttgtggtac ttacgcggca
     6421 gcagttctcc ttgcctatta tcaagactat cgtaaggggt ggatgcttcc aaatgagatt
     6481 cgcaagaaaa atacggctga ttcgttggtt ttgacgaagg cattgcgtag ccagatccaa
     6541 ccactaggac ttcctacgat tccttttcag gttagtacag ggatttctag ttttttaaag
     6601 aagaatggca atcatgagcg tgcacgagca actcttctag gttcatggca gcgagctaca
     6661 aaacgtatac gtgaaggcaa acctgtgatg attggtattt tgaaagtact tggcagtaca
     6721 tatggcaatc attgggtgac agcgtacgct tattttgaaa ctgaaacagg agaacgttat
     6781 tacaaagtac acgacaactg gggcgattat cataaagtca ttcctgccag ttggagtaac
     6841 ggaacagttt cacttcctta agaatttgct gaaattaaca gaaaatcttg gaaaaatatg
     6901 ttaaactatc gaagaataga gaagttttgg ggaggactac atgagtaata agtatcagga
     6961 tgacacgttg aagattttta gtttgaatgg caaccgtcca ttagctgaaa aaatcgcgaa
     7021 agtgttcgga acagaattag gtaaaagcgt cgtgaagcaa tttagtgatg gcgagatttc
     7081 catcaatatc gaagaaagta ttcgagggga tcatgtgtat atcgttcaat caacgaatgc
     7141 acctgtcaat gattactata tggaactact gatcatgatc gatgcaatga agcgagcaag
     7201 tgcgaaaacg atcaatgtcg tattgcctta ctatgggtat gctaggcaag accgcacggc
     7261 gaaacctcat gaaccgatca ctgcgaaatt gatcgctaac ttgatcgaag aagcaggggc
     7321 aactcgagta ctgactttag atctgcacac ggttcaagta caaggattct ttgatattcc
     7381 tgtagataat ttatttacga tgccgttgtt tgctcattat taccgtcaat taggtctgac
     7441 gggagatgac atcgtagttg tttcgccaaa aaacagcggt gttcagcgtg cacgtagtct
     7501 ttcagaatat ttgaacagca cattagccat cgtagatcat gcagatgatg cttcaactga
     7561 gggtggttat gtgatcggtg atgttcaagg aaagacatgt atcatggtag atgatatctt
     7621 gaacacagga gcaacgcttg cacgagcagc aaatgtatta aaagaaaacg gcgcaaaaga
     7681 agtatatgcc tgtgcgtcac atggattatt gtccgatcat gcgaaaagta ttttagacaa
     7741 tgcaccaatc aaagatattt gtatcactga ttctgttttc acagaagaaa atcgtcatcc
     7801 agaaaactta acaatcgtta catgttcaga attaatgggt gaagcggtca aacgcatcca
     7861 tgaaaataca ccgatgagtc cgttattccg tctggaagaa aaagaattcg aataaacaaa
     7921 aaaaagaggg aaagaagttg tgacagaagt gagcagcttc aaggagctgc gacaagactt
     7981 ttgtcacagc tccttttttc tatgcccatg aaacgtcctc tagacaaaaa tgagctcttc
     8041 tttggtttgt tcgctatttt atgctagaat gtgcctagca ataatttgga agggatgaat
     8101 gaagtgaaaa ctatttatct ggatcatgcg gcaactaccc ccatgcatcc acaagtaatt
     8161 gaagcgatga cggcaatcat gcaagacacg tttggaaatc cttcaagcat acatggattt
     8221 ggtcgccacg cgcatgaaaa attggaaaca gcaagacaaa tcattgcaga cagtctgcaa
     8281 gcaaaacctc acgagatcat ctttaacagt gggggaacag aaggggataa tacagctatc
     8341 ttagaaacag cgttctcgcg tcaaaaagaa ggcagacatc taatcactac agctatcgaa
     8401 catcctgctg ttcttaatac catgaaatat ttagagacaa aggggtttga cgttacatat
     8461 ctgcctgtag atgaaaaagg gaatctgtcg atcgaagagg tcaaaaaagc gttacgagaa
     8521 gatacgatct tggtctctgt gatgtacggg aacaatgaaa tcggaaattt gatgccaatc
     8581 aaagaaatcg gtgaactttt agcggatcat ccagcttatt tccatacaga tgcggtacaa
     8641 gcgtacggaa accaaacgat ccatccagaa gaattaggaa tcgatttatt aagtatctct
     8701 gctcataaaa tcaatgggcc aaaaggcgtc ggtttcttat ataaacgaga cggagtaaat
     8761 atccctgcat tgcttttagg cggggagcag gaagaaaagc ggcgtgcagg tacagaaaat
     8821 ttagcaggga tctggggtat ggcaaaagca gttgaactgc tgtcacctga agaacgtgaa
     8881 gcacgtaaca acaaatataa aggattccag caattgattc ttgatacatt agaagcaaac
     8941 gaaatccctt atcaagtaaa tggtgattta acgaataaac tccctcatgt gttgaatctt
     9001 cgcttaattg gcgaagcaaa tgatttgcta ctgatgaagc ttgatcttaa tggggtcgct
     9061 atttctactg gttcagcttg tacagcagga aatatcgagc cttcacacgt attagaagca
     9121 atgtatggag aagatacacc gatactaaaa gaatccatcc gtatcagctt tggtttggga
     9181 aataccgaag acgacatcca gtatttcctt gatcaattag ttcgtgctgt caaaaaataa
     9241 ctagtgaagt cattcttata tgaacagatg tctgagagaa cgccttcatt ttttagggaa
     9301 cttaactgaa aaacctagta aaaatcagga taaactttta taataaagag aaactaattt
     9361 tatcgaggtg aaaatcatgg catttgaaac aacagcaacg ttactgggcg caccaattag
     9421 ctatcgtttg catcctgatg caaaaagata tacattgcgc gataatggat ttacagaaac
     9481 aaacggaggc aattatcaat tgatccgacc attagatgcc acgccgcaaa gcaaagaagg
     9541 attcaaatta aaaatcactg tatcaaaaga tattcaaaca ttgaaaatgt cgatcactac
     9601 agcaaatgga ttgaaagcag tcaatatttt caaagatcca aaacagaaaa tgagccaaga
     9661 taaattttat tttctgatgg atgggatgat tagccgtggg ttattcgaaa aagtcgaatg
     9721 atctgtggat ataaaaagac aactagtttt gaaacggcct attgttagat gactaacttt
     9781 gtaaggacga cgcaaattag ttgtcttttt tatttttaaa tttaacacat gttgttattt
     9841 ttaatatttt gctgatttat tgtttctaag taaagaaatt taaattgaaa cgattgttat
     9901 ttagggaaaa aggtattttt tgaaaaaaga atcccgtata atgattgttg tgcaaacgct
     9961 atacaaaatg aaaaagaaaa gagggatgtt tatggaaaat gaacagtcgg tcgttttaac
    10021 gaattggaag agaaattatt tgttcttttt atcgggacag tttttgtcag gaatcactag
    10081 tatggtcgtc caatatgcga tcatctggta tttgacaaga gaaaccggtt cggcgacaat
    10141 tttgagtttt gctactttac taggaatgat tcccatggtt ttactaagcc catttgtagg
    10201 accgctagtg gatcgctggg ataaaaaagc tcttttgatt gtcacggata ttattgtagc
    10261 gattttcgcg cttattttag cagtagtcgg aacgatttca gaatccttcc cgatttggct
    10321 agtttttgtg tctttgttta tgcgttcggt cgcacaaaca ttccaaatgc caacgatcca
    10381 gtcgattatg ccgacaatcg ttccatcttc tcacataaca aggacgaatg gacagttagg
    10441 aatggtccaa tcagcgaatt tcatcattgc gccagctcta ggtgcggctt tgttttcagt
    10501 cgttcctgtc aattacctta ttttattaga tgtattgggg gcagtttttg gtgtcggctt
    10561 attgattttt gtgaagatcc caaaagtttc tcctgaaata ttggaagttc cgcttactat
    10621 ttttaaagat gcaaaatttg ggctgcagca attgatggat aataaagggt tatggtacat
    10681 aacgatcaat ggtgcatttg tgatgctgtt gttcatgcca gcaatcagtc tgtatccatt
    10741 gatgacgcta gattactttg gtggttccgt tggacaggcg ggagcagtgg aagtagtcta
    10801 cgcagtcgga atgctcttag gcggtgcact gatcagtttt ataggaacat ggaaagaccg
    10861 aatgaaacct attattatag cctacatcat tatgggcctg acgatcggtg caagcggatt
    10921 ggttccaaac gatagtcaag ggttcttgta ctttcttatc ttgaatgcag gagcaggttg
    10981 tgcaacgcca tatttcaata cactgctgat ggcaatgatc cagcagagtt atgaatcgaa
    11041 tgttttaggt cgtgtcttag ggaacttcaa ctctttgatg aacctggcag gtccaattgg
    11101 attattattt gcaggaccat tagctgatcg tttaggggta gaaaaaatgt tcttgttttc
    11161 cggaatcggg attttattat gcggaatcgt tttattttta acgtcagctg cccgaaatta
    11221 tgataaagaa ttacagaaaa aactggtaaa agaacaccat gagcaaaaag acgaatagta
    11281 tggaatggag ttgtgacaga agtcttgtta caactccatt tctgatccta atgttcctgt
    11341 ggcaataagc ctactcaaat gaaaaatatg agtaattatc tttatttgtt taatacttga
    11401 tatagggcag ttttttcacg acctatcgta cttcctcttt gcttcataga tttctttgat
    11461 ttgttgctca gataacggag tgccaaatgg tagtttttcc tttaagagtt ctctttccgg
    11521 tgcgtctacg cctacattga catcgttctc gataggtacg ctgtaatgat ggatatgtcc
    11581 atgaagattg cggatagtgt cgttaggccc gagcaacatc ggataatgag tcaagtaata
    11641 ttgttgatgg tcgaatttaa cgatggcacc tacatcataa aaataaaatt tgggacttcc
    11701 gtcagtaagc ctaggatcat ttttttctaa ataacggaaa aaagcgcgac tgtcatggtt
    11761 ccctttgatc aagtggattg ttccttgcag ttgagtgacc agttccaaaa tttctgcatt
    11821 tgctttttca tattgcggat gcatagcaag gtcgcctaga tgataaacat ggtcggtttc
    11881 ttttattact gcattccagc tagtgatcaa ctgttggttc atttcatcca catttgcaaa
    11941 gagccgcgga gcaaagtcgt tttttcctaa taagcgttca tggaaaaagt gcatatcact
    12001 gataaagtaa ttcatgtatc ttttgcctcc cttttttctt agagtaaaca aaatcactta
    12061 tctgagcaac tcataacatc taaaaagaaa aggttgtaaa tgagacgtgc agctttaagc
    12121 gataagaagc aattttgaaa aatagttttt cacaatttat aaaattattc cttattgccg
    12181 aaaggctggc cctttttatg agtatgccaa ttttttatat cgaatgctca aaagatgagt
    12241 gattttttgg ttgactatca tgtttgtgta ctgaataggt ccagtagaac agtattttta
    12301 cgacttgata attaagggat gaaattttca gaaatatgac gtataatgta aaatactgaa
    12361 aagttgcgac cttcaactcg tagatgtttc agaatctaat tgaaatgatg gtgatatcat
    12421 gacagacaac agcaaaacac gcgttgtcgt agggatgagc ggcggtgtcg actcatctgt
    12481 aacagcactt ttgttaaagg aacaaggata cgacgtgatc ggaatattca tgaaaaattg
    12541 ggatgacacg gacgaaaacg gtgtatgcac ggccacggaa gattataagg atgtagcgaa
    12601 agtagctgct caaatcggga ttccttatta ctctgtgaat tttgaaaaag aatattggga
    12661 tcgtgtattt gaatatttcc tagccgaata ccgcgcagga cgtacaccta acccagacgt
    12721 gatgtgcaac aaagaaatca aattcaaagc attcttggat tatgcaatgg atcttggcgc
    12781 cgattatgta gctactggac attatgcgca agtcactcgc gatgaaaacg gcgtggtcca
    12841 catgcttcgt ggagtagaca ataataaaga tcaaacttac ttccttagcc aattatcaca
    12901 agaacagctt tcaaaaacga tgttccctct aggcggcatg gaaaaatcag aagtacgggc
    12961 aattgctgaa cgcgcaggcc ttgcgactgc taagaaaaaa gattcaacgg gaatctgctt
    13021 tattggtgaa aagaacttca aacaattttt gagcaactat ctgccagcaa aaaaaggaaa
    13081 tatggtcact cttgatggtg aagtcaaagg acaacatgct ggcttgatgt attatacgat
    13141 tggtcaacgt caaggactag ggatcggtgg cggaggagat tcgcaagaac cctggtttgt
    13201 tgtaggaaaa gatctagcga caaacacatt atatgtgggg caagggttcc atcatcctgc
    13261 attgtatgcc acaagcttgg atgcaagtga aatccacttt acgacgaatg aaccaatgcc
    13321 aaaagaattc aagtgtacag cgaaattccg ttatcgtcag caagacgttc ctgtgacagt
    13381 tcgcttattg gatgataatc gtgcagaagt cgtattcgac gaacctgtac gtacaatcac
    13441 tcctggacaa gcagttgtat tttatgatgg aatggaatgt ctgggtggtg gtttgatcga
    13501 ccacgcatat caagaaacaa aagttttaca atacgtttaa gcgatattaa atgaatcaaa
    13561 acagctatgg ctaacaaaag aaacaccttt tcttttggat agcatagctg tttttttgca
    13621 ataaaattaa tttttattca ttttatactt gaaatacttg tgtaaaaagt tcataatatc
    13681 caataatatg aataaagttg ctttttgtta agcaagagca gttgcaaaat agaagggaat
    13741 cttttgcaag tgatttgttc aattttataa gtgagggtga tggaaatgga aaagaaatcg
    13801 acgggtatta gcaaatgggg actggttgct ctggttgtca gttcgtcaat cggaagcggt
    13861 gtgtttggga tcacaagtga tctggctagt tctgcagctc cagggccagc gatcctttca
    13921 tggataatcg ttgggattgg tattttagct ttggtattat cgctgaatca tttaggagaa
    13981 aagcgtccag atctagatgg tggtattttc ggttacgcgg aagcttcatt tggtaaatta
    14041 ggtggcttca tcagtggttg gggttactgg ttgtctgcgt ggctgggaaa tgttgctttt
    14101 gcaacgatgc tgatgagcgc catgggagaa tttttcccga tattcaaagg tggacaaaat
    14161 gttccttcta ttttatttgc aagtattttt atttggggtc tgactttatt agtaaacaat
    14221 ggagttgaag gagcgagcgt catcaataca atcgtgacga tctgtaaatt agtgccatta
    14281 ttcctatttt taatatttgt atttatcgca tttaaagtga atatattcac tgctgatttt
    14341 tggggtaacg tatctgataa tctagtgaat ggaacagggc agcaaggaac gatctggttg
    14401 cagattaaag gttgtttgat ggttatgatg tgggtctttg tagggatcga aggcgcaagt
    14461 gttttagcca accgcgcgaa gaaacgttca gaagcccagc aagcatcgat cattggtctg
    14521 ctttgtttat tgtttatcta tatccttgct tcgatcttgc catacggcgt cttatctcaa
    14581 gaagagttag caactatttc acaacctgct atggcaaata ttttaaaagg aatcgtggga
    14641 aattggggag cagcgctgat caatatcggc ttgattattt cgatcattgg ttcttggttg
    14701 tcttggacga tgctcccagc agaaacaacg atgttgatgg ctcgagacgg tctgttgccg
    14761 aaaggttggg gaaaaacgaa taaaaaaaat gccccaactt attcgttagt tgttactgca
    14821 atcttgacta atctattctt attgactttc ttagttactg attatgccta tcaatttgcc
    14881 tattctttat gtacagcggc aattttgatt tgttatctac ttgtcgggat ctatcaagtc
    14941 cagttttctt taaaagaagg aatcaaaaaa caagtagtga tcggtgggat tgctgtagtt
    15001 tttgaattga tgggcatcat tttagcagga ttttcttatg tccttttatg tagtattgcc
    15061 tatcttcctg gtttttgctt ctactggatc gcttgtcgtg aaaccaaaca tcaaattacg
    15121 ggaaaagaaa aagtaatgat tgggctgatt tgtgtggccg cactgctttc aatcgggttg
    15181 cttgctgtag gctggatcaa gatataaggg ggaaagaaac atgaaaatcg caaatttcgg
    15241 tgtagaagaa tggctaaata aatgggaaaa gaaagcggta tatgatattg cacaaagctc
    15301 gatcgaagca ttgacattag acgaactcgt tggcttagat gggactagcg taagtaaatt
    15361 tttcgaaaaa cgcaaaaacg aaccattgga ctatggctgg atcgaaggat cagatgtttt
    15421 caaacaagaa gtcgcttctc tttatcaaac cgtcgatccg gaaaacatct tacaaacgaa
    15481 tggagcgact ggtgctaatc ttttagctct atacgcgtta gtggaagcag gagaccatgt
    15541 tgtttcgatg cttcctactt atcagcaatt atatgacatc cccaaatctt taggtgccgc
    15601 tgttgacttt gttcatttga aggaagaaga agattggcag tttgatttag aacagctaga
    15661 agccttagtc aaacctgaaa caaaaatcat ctgtttgaat agtgcaaaca atcctacagg
    15721 cacattgcta gatcggaaaa ctttagaaaa aatcgcagaa attgctcgta cagtagatgc
    15781 atatgtattg atagatgaag tatatgcgcc tttaacggat aaaggtgaat tcttgtcgat
    15841 cgtagatgtc tatgaaaaag gtatagcaac caattcccta tccaaaacct actccattcc
    15901 aggtattcgt attggctgga cagctactgg acctgagttg gcagaagttt ttcgaaagta
    15961 tcgagactac acgatgattt gcggcggagt cctgtcagat gatcttgctg tacatgcatt
    16021 gaagaataaa gaaaaaatat tggcgcgaaa caaaaagatc attacagaga accttgtgat
    16081 tttaaaacaa tgggtagcca atgaaccgaa agttgaactt gtcgctccca attacgtctc
    16141 tacttctttc attaaattag ccatcgaaga agatgaccag acattttgta tcaatttatt
    16201 agaagaaaca ggagtactat tagtaccagg aagtgccttt gatttaccga agcatgcccg
    16261 actaggttac tgttgtaaaa aagaaacctt ggaaaaggga ttattcctct tatctgagtt
    16321 cttgaaaaaa caggcctaaa caaaaacact gcaaggacag tctgtgacaa ctgttcaagc
    16381 agtgtttttc atttacgctt attgtctatg agaaggagag tgccctgaag ctatggagac
    16441 tcctgaagct ttttcttctg cagtttcttg ctgatggatc tcttgtgtat tgtgtcgtac
    16501 aagcaagaag ttgaccaaaa caagaagaga agtcgaaaga aagacgccgc gataaccaaa
    16561 tgcactggaa acactggagc cgatcatagg ccctactacg ttaccagttg cttggaaaga
    16621 ttggttataa ctgaagatcc gtccagcagc ttgatgaggc gaatattttg tgatcaatgc
    16681 ttggacagca gggagcagac aagcatcaga gataccaatc aaaaaacgta aggcagctaa
    16741 ttgccaaaca ttcgtgacga atgccattgg gatataaacc agaatcgcaa aacccaaacc
    16801 aatcgcaagt attctttcac tcccaatctt gtcacctaag cgaccaaatc ttggagcggc
    16861 tatcaaagtg gcaatcccag gtatcgaagc aatgattccg ctgatcaacg taacattccc
    16921 atgtccatgc agcaattggc gaatgtacag actgatgatt ggactaatgg aattgttgga
    16981 tgcttggatg atcatcgttg tgacgaacat accgatgacg acgtgtggat atttcaagtc
    17041 tttgaaaatc tgtttggcag aaaccatgtt ttcttttttc actgggacaa atttttcatg
    17101 gacgaaaaac agactcaaca agaagacgaa aaataagatg atccctgtaa tgaaaaaggt
    17161 agggcgatag ccaaacgctg aagcagagat cccgccaaga agcggtccaa gtaatgttcc
    17221 tgtaacagaa ccagttgcta aggttccaag aacttgacca cttttttctt ttggcgtccc
    17281 agttgcgaca agggcagtgg cattactgat ataaccagaa aaaatccctt gaagcagacg
    17341 caatgcgact aattgataca cactagtcac gcaacccatc aaagaaatga cgattgccat
    17401 tcctaatgaa gcacgcaaca gcatcaattt tcttcctttt tggtcggcta attttcccca
    17461 ccaaggagag ataagcgtag tcaccaagaa agtagatgaa aaagtcaatc cgctccaaaa
    17521 gttcaattgt gacgtactat aattacctaa cgtatctatg taaagtgaca taaaaggcat
    17581 gactaaacta aatccgatac cagccataaa tgttccaaac cataagacaa acaagttttg
    17641 ttcccaagtt tctcttttcc taaataattt ttcctttatt cttgcagcca attagactcc
    17701 ttctttctga aaattttcat ctacagctct ttattataaa ctacaatatg tagtttatca
    17761 atgaaagtct cttttttctc tttctttttc gtggtaaact gaaagagtga aggatttatt
    17821 ttatgagagg aaacatggaa atgaaaaaaa gaaatctgtc tcaagagaaa atcatcgact
    17881 gctttcgaga actagcagaa gagatggatg ttcagcaaat aacctttcag catttagcca
    17941 aacgcttgga tatcaaggct ccctcgctgt ataaccattt caaaaatatt cgtgatgtca
    18001 aaacagcgct aacagcgaaa cttctgcagg aacttaatga ccagctgcga cgggctcttg
    18061 tgggaaaaag tggtcaggaa gcactgcaga tctatgcaga aacttatcga tcctttgctt
    18121 tttcaaatca agcagtgtat gagttactga tcagtgtacc gcataccaat gaagctgttt
    18181 tattagatgg aatccatgaa acgaatcaga tcattttgca gatatttgag gcttttcaat
    18241 taacgaaaga agaaaaagtt catcgaagca gggaacttcg tagtatgatc cacggttatc
    18301 tttccttacg ttttttagga tactttacaa aagaaccaac aatcgatcca gaagacagct
    18361 atttttggat gatcaataac tttatcgcta ctttgcctta aacaatcaga ttcatttgta
    18421 acaattttaa gatgttcatt aaatgaccct tttataagag aaaattcttg caactttctt
    18481 gaaaagtgac taagatgagt ctagaaaagc tgaaagaagg agcacgagta tgtatcaagt
    18541 aattaccatg ttcggggaca atgagccttg gtggtttttt gaagactggg aagaagatat
    18601 tgaagaagaa gaaacattcg agtctttcga agaagcacga caagcgtacg aaaaacaatg
    18661 gaacgaaatc catcaaaatt atgaatatat caatgcaaaa agtaactttc tcagcgcctt
    18721 ttggaatgac ggagatgagc gctggtgtga agagtgtgac gatgatttac agcaatacaa
    18781 gggacttgcg ttgttgaaaa atcatcagcc catcacagta gaaagcagaa aggaatttta
    18841 tgaaacaact aattctagcg gaaaaaccaa gcgttgcgaa agacctaagc aaggtgcttg
    18901 gtgctaatca aaagcacaaa aattattatg aaggacccaa ggtgatcgtt acttgggccc
    18961 ttggtcattt attaggattg aaaatgccgg aagatctgaa taaggaatgg cagagctggc
    19021 aaatggacac gttgccaatg atccctaaaa aactaggaat caaaccactt cctaaaacag
    19081 gacatcaatt aaaagcaatc aagcagctgg ctcagcgaaa agatgtctca gaagcagtca
    19141 ttgcgacaga tgccggaaga gaaggggaac ttgttgctcg ctggatctta gagtatgtgc
    19201 atttcaataa accagtgaag cggttatgga tctcttctca aacagataaa gcaatcaaaa
    19261 ctggattcaa gcaactaaga cctgcgaaag aatatgatgc attgtatgaa tctgctcttg
    19321 cccgggcaaa agcggactgg ctggtaggat tgaatgtgac cagagcctta actgtgaagt
    19381 acgcggataa tttatcagca ggccgtgtgc agacaccgac actagcatta gtgagagacc
    19441 aagaaaagaa aatcgagcag ttccgtccac aaacgtattt cacgatcacg ttgcaagctg
    19501 gaaaagaaca agccaaaatg cttcaaaaaa atcaatttgc tttaaaaaca caagaagaag
    19561 cacaaaaact tgttcaattg atcagtacaa gcaaaggaac tgtcacagaa gtccaagaaa
    19621 aattaaaaac ggaatctgcg ccattgcctt atgatttaac agagatccaa agagaagcca
    19681 atcaacgcta tggctattct gcaaagaaaa cattaagtct tgtacaaagt ttgtatgaga
    19741 cccataagat cgttacttat ccgcgtacag acagcaaata tctgacaaat gacatgaaaa
    19801 gtacgatgaa agaacgattg caagcagtca gcgactttgc tcctgaggtc aagcagtatt
    19861 tgaaaaacgg agccatcgtc caacagacaa aagtattcca agacagtaaa gtgaccgatc
    19921 accatgcact tttgccaact gaaaacagag cacgttatga aaaattaagt aatgaagagc
    19981 gaaaaatcta tcaaatgatt gtctctcgtt ttttaggact gtttgctcaa ccccataaag
    20041 tcagccaaac aaaagttacg gttgaatttg gtaaagaacg attcattttt cggcaaaatc
    20101 gtgtcgtcca agcaggatgg aaaggcgaaa ctgaaactga gacggaaaca gtcaaatggg
    20161 aaaaaggaat gagaatcaat cccgatttcg ccatcaaaaa agaactatct gcaccgccta
    20221 aaccattgtc agaagcaagc ttgcttggtc aaatggaaaa atacagtctg ggaaccccag
    20281 ctacgcgtgc agagattatc gagaaactaa tcaaatctga actgatggaa cgtacccctc
    20341 aaggactgca agtatctcct aaaggtaagc aattattagt attggtaaat ccctctttag
    20401 taacaccaga tttgacagaa agctgggaaa aagaccttga agctattgct gcaggaaaga
    20461 aacaagcagg aaccttcttg aatggaatcg aaaaagaaac aaaacgcttg gtgaatgaga
    20521 tcaaatcaag caaacaagaa tatcaagatt tctcgattac ccaaaagaaa tgcccagaat
    20581 gtggggccaa tctgcgcgag aaaaacacac gtgacggcaa aatctatgtc tgcaccaatt
    20641 cagattgcaa ctaccgcaga agaaaagatc caaaagtctc taatcatcga tgcccgcaat
    20701 gtcaccggaa aatggaaatc atcgaaggta aaaacggtgc ttatttccgt tgtaaatacg
    20761 atggaacgac agaaaaattg cttgataaaa aagagcagaa gaagaaaatg accaaacatg
    20821 aagaacgacg cttgctgaaa aaatatgcac aagcagaaga accagaagaa agcccattag
    20881 cagcagcact aaaagcagca atgaagaagt aataagatat gaaagaggtt gtgatagaag
    20941 tggtcagctt caagaaataa gaaggaattt acgaaaattg cttctcaaat ttttgtgaaa
    21001 tttcggctta tttccgaaga agctacttct ggaacaccat ttattcggaa aaaaggactg
    21061 tgacaagact tttgtcacag tcctttttca tttaaaaaaa tgtttacttc atttgacgta
    21121 tattattggt gaggcttcaa ttcatttggc ttcacttttt ctgtttctct tatgatataa
    21181 acaggacgct gtttcgtttc taagaagatt ttcccaatat atttcccgat gattccaaga
    21241 cataacaatt ggattccacc aatgaacagg aagatcgaga ccatggaagg ccatcctgat
    21301 gtaggatcac caaacaacaa tgcccgcaaa ataatgaaca aaattgcaat accagaaccg
    21361 acacaagaca aagcgccgat aaatgaagca atcgttaacg gtgcatccga gaagttcacg
    21421 ataccatcaa tcgagtagtt gaataatttc cagaaggacc aagatgtttc gccagccaca
    21481 cgttcgcggt tttcataaga aatatattct gttttaaatc cgacccagct aaataatcct
    21541 tttgaaaatc ggttgtattc tgttaattct aggacggcat ccaccatttg acgagtcatc
    21601 aagcggaaat cacgagcgcc atcgaccatt tctgtatcgc tgattttatt gatcaactgg
    21661 tagaattttt tcgcaaagaa actgcgtatc gcaggctcgc cttcacgagt cgaacgccgg
    21721 gtgccgacac aatcgatatc agaagtagtg atccggttga tcatctcagg aagcagttcg
    21781 ggcggatctt gcagatcaac atccatcacg gtcactaaat ctcctgtaga agcttgcaat
    21841 ccagcgtaca gagccgcttc tttcccaaaa tttctggaaa atgagacaaa gcggacataa
    21901 ggataagctg aggctagctt cttgattgct gctaacgtac cgtcttttga tccgtcgtca
    21961 ataaacaaat attcgaactc atgggcacct ttgaattttt caacctcatc aaaaaaaaga
    22021 ggaacagttt tttcttcatt aaaacatggg acaacaattg atactttcat cgtttctatc
    22081 ctttcaatcg tatgtttata tatggtttac attcaaaatt taataaacct taaataacac
    22141 ctttaaaatt tgattttacg taaaagaaca ggtattcttt ttgtaaacta acgtatttag
    22201 tttaccatgt ttcctcggtt tatcaaactt ttaagaacaa tttttattag ttggcaagta
    22261 gttttggata gagaaaaagg aggaagttcc agtctattga ctaggagcaa acaaaatgaa
    22321 aatacacaca cctatcggct ttttatggtt agatgcagat gaatccggat tgactgctgt
    22381 atcttttcag ccaatttcag aaagcgaatc agcgaatcag cgaattttaa cgcgcacaca
    22441 agaacagttt gaagaatatt ttgcgggaaa aagaaaagaa tttgatgttc ctttatctat
    22501 aaagagaggt acgccttttc agcaaaatgt ctggaaagct ttgtcgacca ttccttatgg
    22561 agaaatccga acgtataaag aagtagccgt tgctgttgat agtccgaaag ctgtgcgagc
    22621 gattgggcag gcaaatcgaa ccaatccatt gccaatcgtt attccttgtc atcgagtgat
    22681 cgggcaaaat ggaaaactga caggatatat gggaaacgca atagatgggg ttgaaatcaa
    22741 acgccaatt
//
LOCUS       DAYYGS010000046        22713 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000046 DAYYGS010000000
VERSION     DAYYGS010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.046"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..210)
                     /locus_tag="ACHA44_001631"
     CDS             complement(<1..210)
                     /locus_tag="ACHA44_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904907.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIN"
     gene            complement(301..753)
                     /locus_tag="ACHA44_001632"
     CDS             complement(301..753)
                     /locus_tag="ACHA44_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HFM8904908.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(766..1176)
                     /locus_tag="ACHA44_001633"
     CDS             complement(766..1176)
                     /locus_tag="ACHA44_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8904909.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            1451..1663
                     /locus_tag="ACHA44_001634"
     CDS             1451..1663
                     /locus_tag="ACHA44_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFM8904910.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            1666..1878
                     /locus_tag="ACHA44_001635"
     CDS             1666..1878
                     /locus_tag="ACHA44_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904911.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            2018..2173
                     /locus_tag="ACHA44_001636"
     CDS             2018..2173
                     /locus_tag="ACHA44_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904912.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(2170..2394)
                     /locus_tag="ACHA44_001637"
     CDS             complement(2170..2394)
                     /locus_tag="ACHA44_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8904913.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            2472..2660
                     /locus_tag="ACHA44_001638"
     CDS             2472..2660
                     /locus_tag="ACHA44_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904914.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            2799..2927
                     /locus_tag="ACHA44_001639"
     CDS             2799..2927
                     /locus_tag="ACHA44_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904915.1"
                     /translation="MAREEALKIGKVIADNRWGNSRPIILSKQHIEKQKAWQQIKK"
     gene            2986..3321
                     /locus_tag="ACHA44_001640"
     CDS             2986..3321
                     /locus_tag="ACHA44_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904916.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            3554..4495
                     /locus_tag="ACHA44_001641"
     CDS             3554..4495
                     /locus_tag="ACHA44_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda-exonuclease family protein"
                     /protein_id="HFM8904917.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            4497..5387
                     /locus_tag="ACHA44_001642"
     CDS             4497..5387
                     /locus_tag="ACHA44_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFM8904918.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            5430..6230
                     /locus_tag="ACHA44_001643"
     CDS             5430..6230
                     /locus_tag="ACHA44_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFM8904919.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADNIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            6245..7096
                     /locus_tag="ACHA44_001644"
     CDS             6245..7096
                     /locus_tag="ACHA44_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8904920.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            7297..7758
                     /locus_tag="ACHA44_001645"
     CDS             7297..7758
                     /locus_tag="ACHA44_001645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8904921.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            7784..8236
                     /locus_tag="ACHA44_001646"
     CDS             7784..8236
                     /locus_tag="ACHA44_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904922.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            8342..8536
                     /locus_tag="ACHA44_001647"
     CDS             8342..8536
                     /locus_tag="ACHA44_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904923.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNGFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            8567..8953
                     /locus_tag="ACHA44_001648"
     CDS             8567..8953
                     /locus_tag="ACHA44_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904924.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQKIVLEWLKSETILTREAPILSVNAFSDKNLLGKLPDKVRKAY
                     KLLACKQEYEVLSAFAQWGLEQEEAE"
     gene            8950..9153
                     /locus_tag="ACHA44_001649"
     CDS             8950..9153
                     /locus_tag="ACHA44_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HFM8904925.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASNAIRKFGISILNDDRKWEEMR"
     gene            9150..9446
                     /locus_tag="ACHA44_001650"
     CDS             9150..9446
                     /locus_tag="ACHA44_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904926.1"
                     /translation="MKIKDGFYASSHGIGGLMLDMPIKNPKTRQKSKFKIGDMVRCKA
                     EEFIYPFRGYVEHVYNHSAIIHIENTMECDKWLAKSKDNLAVARLVDMELINDK"
     gene            9522..9938
                     /locus_tag="ACHA44_001651"
     CDS             9522..9938
                     /locus_tag="ACHA44_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family phage packaging/lysis
                     transcriptional regulator"
                     /protein_id="HFM8904927.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
     gene            10199..10336
                     /locus_tag="ACHA44_001652"
     CDS             10199..10336
                     /locus_tag="ACHA44_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904928.1"
                     /translation="MDKEIKVTVKLDLTELKELLNKASDQVEQLQETLDEIANFKIQV
                     S"
     gene            10746..10976
                     /locus_tag="ACHA44_001653"
     CDS             10746..10976
                     /locus_tag="ACHA44_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904929.1"
                     /translation="MRNYWYISLTNEYPRTIDDCSVRVVRSVQIKKKYSIVEMTREAT
                     PKEIDKCKLLYCGHGFYDEPNIQNNINKNLRD"
     gene            11135..11968
                     /gene="terS"
                     /locus_tag="ACHA44_001654"
     CDS             11135..11968
                     /gene="terS"
                     /locus_tag="ACHA44_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFM8904930.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELKSNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPKRNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            11961..13376
                     /locus_tag="ACHA44_001655"
     CDS             11961..13376
                     /locus_tag="ACHA44_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFM8904931.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPENMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTTVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            13388..14917
                     /locus_tag="ACHA44_001656"
     CDS             13388..14917
                     /locus_tag="ACHA44_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFM8904932.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEYMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKDPTAKAEYVTKKLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            14835..15887
                     /locus_tag="ACHA44_001657"
     CDS             14835..15887
                     /locus_tag="ACHA44_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFM8904933.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            15889..16206
                     /locus_tag="ACHA44_001658"
     CDS             15889..16206
                     /locus_tag="ACHA44_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904934.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            16333..16956
                     /locus_tag="ACHA44_001659"
     CDS             16333..16956
                     /locus_tag="ACHA44_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HFM8904935.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            16971..17861
                     /locus_tag="ACHA44_001660"
     CDS             16971..17861
                     /locus_tag="ACHA44_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 family protein"
                     /protein_id="HFM8904936.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            17884..18099
                     /locus_tag="ACHA44_001661"
     CDS             17884..18099
                     /locus_tag="ACHA44_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904937.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            18111..18452
                     /locus_tag="ACHA44_001662"
     CDS             18111..18452
                     /locus_tag="ACHA44_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904938.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            18452..18823
                     /locus_tag="ACHA44_001663"
     CDS             18452..18823
                     /locus_tag="ACHA44_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904939.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            18816..19211
                     /locus_tag="ACHA44_001664"
     CDS             18816..19211
                     /locus_tag="ACHA44_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFM8904940.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGAVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            19213..19590
                     /locus_tag="ACHA44_001665"
     CDS             19213..19590
                     /locus_tag="ACHA44_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6838 family protein"
                     /protein_id="HFM8904941.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNKPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            19591..20199
                     /locus_tag="ACHA44_001666"
     CDS             19591..20199
                     /locus_tag="ACHA44_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HFM8904942.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            20199..20540
                     /locus_tag="ACHA44_001667"
     CDS             20199..20540
                     /locus_tag="ACHA44_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904943.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGPVVEEAAEVMEG
                     MQNAVDGKSVSEALSRAYSFIADILFEGQFTGEDYRQGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            20782..22713
                     /locus_tag="ACHA44_001668"
                     /pseudo
     CDS             20782..22713
                     /locus_tag="ACHA44_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT         8207 a         3306 c         4672 g         6528 t
ORIGIN      
        1 attaatatca ttttttaaca tagtaatttg tttatttaaa tctgatagtg tttcttcctt
       61 acttaatata aatctttctc tatcatgtaa tttatttagt ttttcatcat ttttttgttc
      121 taattcataa gcttcttttt ttattgtgtt agctttcgac agtttagtga ttcctataat
      181 taataaaata agtccaatca ataataacat tcaaaactcc tcatttcttt gatataatta
      241 ttgtagagtt tactcagaaa tgaggaaaga gtccatgtga aagcatgggc ttttttattt
      301 ttaatgttgt aatactaaat gttcaagatt atgaggaagc cctaaatatt caataatttg
      361 gaactgactc atatcagata aatcttgttc atctattgtt ctagttaaca tttcaattgc
      421 aaacatatta gcttcagctt ctataccata aatcatacaa ctaaggttgt ttgctctaaa
      481 agctgctgtg caaatacctt tatggagtct aacgtgggct atttcatgcc ataatacaaa
      541 tcttttcata gaaggtgata ggttttgatt aattaatatc acataacatc ttttattgtg
      601 tatcgtttgt cctaatgttt tattccctaa atcagtttca attaaatcta tatcagaata
      661 tttgattatt ttatatggat taatcgcatc agatccaaac tctacaaata atttttctat
      721 ttcttctaat atccagtatt tgtttaaatg cataccaaag cccacctatt gacgatattt
      781 tttaggagtg aattttttct ttgctttaac tttggttaag gcaagtgcaa gttgaatggc
      841 atcgtataat ttttccttat cttcatcgtc taaaggttca ccataataat ttgtttcagc
      901 atctgaattc aagccttcaa gtaatttttc agcttgtaaa gcaatatctt gtttatcttt
      961 ttctgttaaa tcataataat ggcgtttatc agtacgtcct aaaagataat cagttgatac
     1021 attaaagtaa tctgcaactt tttgtatttt atctccagat ggagtacttt tattccatct
     1081 agacatgctt ccatttccga aatttaactt tctttcaagt tctgccaacg tcattgaacg
     1141 ttcttttaat aagattttta tacggtctaa tagcatagga tatgcattcc tttcaaaata
     1201 ccgacaaaaa aagtattttt ctgcaaatta tctatttaca aatagagaat ttgcagttat
     1261 actagttttg taagcaagat atttacggca tacaaagact aaaagcaata aactaatagt
     1321 ctccccagac atcagtaagc tcgctttttg tgcgctgtat tttgtatgct tacataatag
     1381 catatttgca gtttttgtca aacttattag acaaaaatat tagaaaatat tcgataaggg
     1441 ggtatgaagc atgactttaa aacagcaagt attaattgct ttagtaaaga aaggttggag
     1501 tcaaagagag ttagctcgac gaatgggtat atcaattact tatttacgag atatcttcat
     1561 cgaaaaaaga aaacctaagg aacgtttaaa acaaatcgaa gaacttttgg atatcaagtt
     1621 agaagtagat tctaatgatc agccatcaga acaggaggcg taaaaatggt acaacgtttg
     1681 agcaaagcgg agtttaaaga gaaatataag tattctgaaa gtacttatca aagaagaatg
     1741 aaggaattta aaaaatcacg ttttagtgag gggtataacg cagttacttc caaagaaata
     1801 acaattgatg tcgatttata tgaacagttt agaacatggc gttctaaaaa tcgatttaga
     1861 gttagagctg tttgttaata gctaaaaaat ctaaattcac cacttctgaa aagaggagtt
     1921 gctgctctcc tttcaagttt gccaaagtcc acgttttttc attttggcaa ctcctcttct
     1981 tggaggtggt gacggcgaga gacgaaggag ggatgacatg aaggaagaat caaaaatagg
     2041 gattttgaat gagaacaatt acacaagatt aattattgaa acagaagaag ggaaaaaagt
     2101 agcagaagtt acattgaccg atgcaactcc tgctgacggt tatcgtattc gattaactcc
     2161 taaatatgat taaccttttg gtgggtgtgg atcgtttccg tgactatcac ggctttgaat
     2221 ttttccatct ctgccatgaa taaatagctc agatccttga tttttagaaa tctctctagc
     2281 gatttttact gcttctgatt ttgtattagt atgtgcagtc gctttagaat ttccagcacc
     2341 tttgacattc caaccaccat tggaattagg tacaacatgt tgattttttc ccatagtttt
     2401 cacctccatt tctatagaaa tattttatca aatagaaatg gagaaattca ataaacgaaa
     2461 ggagaagtga tatggcatat accttgcaac aagaacatca aattctcggt ttgattaaac
     2521 aacgcaggaa acaattacaa gatgaccgtg cagcgcttag aaaagccgat gagctatcag
     2581 ataggcaagc tgaactaatt gcttctgaac ttgaggattt gagaatgcta gaaataaaaa
     2641 atagggagat tagattatga agaagacaga cacacttttt ataggattca ttttggggtt
     2701 attagtgatt gtagcgcacc aaagtattat cgggggaagc ttgttcgcag cattgatggt
     2761 tttaatcaat ctgcttgatt caaaagaaag gagcaactat ggcacgagaa gaagcgctaa
     2821 aaatcggtaa agtgattgct gataatcggt ggggaaatag ccgccctatt attttaagca
     2881 agcaacatat cgaaaagcaa aaagcatggc aacaaataaa aaagtgactc tgccagccag
     2941 caaagagtca catacaaatt tataaaacta ggagaattat atcacatgaa taagaaaatt
     3001 gaaaatctta tcgaagaatt gaaacgtgaa tgccaaaagc aaggtgtttc tattatttgt
     3061 acagcccaaa aagaagggga acttaaaagt ctcgtttatg gtgagacaac agaaatttta
     3121 ctttgtcttg ctatgcaaga agaacattta gatgaaaatc tcccattgtc agcacatatt
     3181 atgcgtagaa ttgctgtgga tgcttacgaa caagcgaaaa atgaagaaga aaatcaacct
     3241 tcaaatcata cttttgttat taataacaag gaagatttag ctgatgtgat gactcgtatt
     3301 ctcaaggggg aatttcaatg atgaatgaaa aaacaattga tctactcatt attttatttt
     3361 tcacgttagt gattgtagcg aaaagttatg taatctttgg cttgttattc gcaattacca
     3421 tgcttgtgct ttgcgtgtta gcaagcagaa aagaggttaa acatgaacga gttagcaaat
     3481 cttgacaact atttaactga tcctgattat gctaaaccac catatgaagc accaattgat
     3541 gaggaggatg aagatgagta aatctacctt agaaatgagc catcaagaat ggcttgaaga
     3601 ccgtaagaaa ggcatcggag gttcggatgt tggaacaatt ttaggattga ataaatggaa
     3661 atctccttat caactatggc tagaaaaaac aggacaagta gtacttgaag aatcaggaag
     3721 tgagcccgct tattggggta atgttttaga agaagtggtt gctaaagagt ttcaggaacg
     3781 tacaggcaaa aaagtacgca gaagaaacca agtatttgaa catccattac atccattttt
     3841 aagagcaaat attgatcggg aagtagtggg agaaaatgcc attctggaat gcaaaacagc
     3901 taatcaattt ctcggtaaag aatgggaagg cgaagaggta ccgcttagct atctctgtca
     3961 agttcaacat tacatgaacg ttctaaacaa agactattgt tacatcgctg tcttaatcgg
     4021 tggtcaaaaa tttatctgga agcggattga acgagatcaa gagctgatcg atacaatcac
     4081 tgaacaatta gtagagtttt gggaaacaaa cgttcttgga ggtatcgagc caattattga
     4141 tggtagtcaa gcgacagctg atttcttgaa agagaagtat gcagatgttg aagacgttca
     4201 aacgacgtta ccaattcatt tcgatgaact agttgaacag aaaaacgaac tcaaacggac
     4261 taagaaagaa attgaatcag ctatccgtca agtagacaac gagattatca gcgagctagg
     4321 taaacgtgaa gccagtatcg gtatcactca aaaaaacatc atcagttgga aatttgtccg
     4381 tacgagacgt atgaactcga agaaactagc agagaaatat ccagatgtcg caaatgatga
     4441 agagatttat aacgttactg aatcaagaag gctaaccgaa agggagatca aataatatgg
     4501 caacaaatga atcgttaaaa aatcaattgg cagcaaagcc acagaaacaa gttgcaccag
     4561 gtcaattagg acttaaagct ttgatgaata caccaacaat gagaaagaag ttcgaagagg
     4621 tgcttcatga caatgctaat gcttttatgt cgaatgttat gactcttgta tctaatgaca
     4681 gttatcttgc agatagtgaa ccgatgtcta tcatgagtgg tgcgttaact gctgcaacat
     4741 taaatcttgg gctagataag aatttaggtt atgcatattt agttccattc aatagtaaaa
     4801 acaagcaaac aggaaaatgg gaaaagaaag ctcaattcat gcttggctat aaaggatata
     4861 tccaattagc ccaacgatca ggtaaataca aagcattaaa tgtgatcgaa gtttacgaag
     4921 gagaactaaa aagctggaac cgactgacag aagagtttga gtttgatcca aatggtagaa
     4981 cgtctgatga agtcattgga tatgttggct atttcgagtt actgaatgga ttcaaaaaaa
     5041 ctgtctattg gaccaaacaa gaaatcgaag ctcatcggat tgctaacaat aaagatcgcg
     5101 ataagacaaa gttaagtggt gtgtgggcat ctgattacaa tgcaatggca cgaaaaactg
     5161 ttttgagaaa tcttctttct aaatggggaa tcttgtccat cgaaatgcaa gaagccacca
     5221 catcggatga gagagtccaa agggttcaag aagacggcag cattattgct gaaacagaag
     5281 ttgaagaaga tattcctgaa agaaaagaag cagaggttat atctgaagaa aatgaagatg
     5341 tacaaactgg attatttgat gcatctaatc cgccgttaaa caaataatga gggagctttc
     5401 tcccttgctt ttctagagga gaaatacgaa tgaacacagg atatgtcaaa ttacatcgaa
     5461 aagtgatgaa ttcattcgtt tggaccaatc cttatatgta taaattatgg aacttgtgtt
     5521 tgatgaaagc gagtcatgag aatcgcaaga tactttttaa tggaaaagag atacaagtga
     5581 acagcggaga attcgtcaca gggcgcgatg ctatcacatc tgagatgaac gaaggcgtga
     5641 aacgtgaaca tcaagtgaac agcggttctg tatggagatg gctaaaacaa tttgaaaaaa
     5701 acggaatgtt gaacatcaaa tcaactacga aatacagcgt tatatcaata aaaaactggt
     5761 ctttgtacca aagcagtgaa caacaaatga acatcaaacg cacaacaagt gaacaacaag
     5821 tgcacacaaa caagaatgaa aagaattata agaatgaaaa gaataataaa acatcatcgt
     5881 cgcaaccacg caaaaagcgt gtttacgaca ccgactcagt ttactacatt ctcgcggagg
     5941 agttattcaa acagatttgt cagaatcagg aaatcaaaaa gccgaatctg caaagttggg
     6001 cagataacat tcggaaaatg atcgagatcg acaagcggac cgaaaaccag gtacggggaa
     6061 tgattgaatg gagtcagcac aatgtgtttt gggcatcgaa tattttatct gctaaaaaat
     6121 tgcgagaaaa atacgacaca atggcagcgc aagccaatcg tgattataaa acaaaacaaa
     6181 ctaaaacgct tgaatacgag aaatttagca cagatgagtt gcctatttga gaggaggcgt
     6241 aggcatggaa actgttgggg aaatcatgga aaagctgata cagaaagtgc ttgtccaacg
     6301 tggcgaatgt cctgaatgcg gacagccttt gtatggatgg cgtacgaaga accctgatgg
     6361 ctcagaacgt tgtaagccaa cctgtatgca atgtgggtat aaagcattgc gagttcagga
     6421 agacttacaa acagaacgaa tttacaacga gagcctgaaa gcaagagcaa tcaatttttt
     6481 caaaggtggt tctgttgtgc ctaatcaagc gttgtttgat tgcacattgc agaattatca
     6541 aattgtcgat caagaaacaa gacaagcggt tgaagtaacc aaacgctttg ttaattcagt
     6601 cttgttagga aatccaagtc accttgtttt aactggaaaa caaggaacag gtaaaagtca
     6661 cctagcgatg gcagcggctt gggaagtctt gaagcgatca aactacgata aaaaaatctt
     6721 atttatcggg ttacaggaaa tgctggatca aatcaaattt tcgtacaaca atcctgaact
     6781 cagaaaaacg attgagggat cgttgattgc agatatcaaa acagcggatt tagtcatcat
     6841 tgatgatatc ggttcagaac taggaaaaga tgcatcagat agtcgagcgt ttggcataaa
     6901 cacgctaaat tcgttcttgg atgcacgaca gaacctagca acgattatca caacgaactt
     6961 gcttggtgaa gaactgaaaa aagcttacgg tacgagaaca atatcaagaa tgtttgtcaa
     7021 ctctgatgga tttacgatgg tattttctca aacagcagac aagcgcataa aaccagtcaa
     7081 aggagcgatc gcatgaattt gactaaacaa gaaaaaagaa tctgtcgtca gataatgttg
     7141 caaattatag gtgatcgtga catggacgaa ttatctcaac aagagagtcg aatggttcgg
     7201 aagataagta agctaattat ttatcgggat acagaaagat ttctcactgt tttaggtaac
     7261 gtctacgaga atccagagtt attggaggta gagtaattgc caaaaatatt agatgcatgt
     7321 tgcggtagta gattgttctg gtttgataag aacaattctg acgtaacatt tatggattgt
     7381 cgtcagcagt atgaagaatt atcaacaggt catgtgatca atgttgatcc agatgtggtc
     7441 gcagactttc gcgatatgcc ttttgaagat gatgtgttcg atatggttgt atttgacccg
     7501 ccacacttaa gacatgtcgg cgaaaactca tggcttgcca aaaaatatgg aaaattagac
     7561 gaactttggc cagaagacat caggcaaggt tttgcagagt gtatgcgagt tttacgacct
     7621 tcaggaacat taatttttaa gtggaacgag gaacagatac cactttctga tgtgttagag
     7681 gctattggag aacagccatt gtttggtaat aaacgaagca aaacacattg gcttgtgttt
     7741 atgaaaaaat tcagctaacg gaggaaacaa aggaggaaaa gtaatgaaga ttgtattaaa
     7801 taaatgtttt ggtggctttg ggttgagcca tgaagcaaaa atggaaattt ttaaaagaaa
     7861 aaatattgaa gtattcccat acatcaataa ctttagctat gactcagatg atgaatacac
     7921 gagatacaca ggccaaaagc taggttcgat ggattttatc tactatttca aaaaagaccc
     7981 gaaaatcgac aaagttacag gaatttatag cgaaattgac cgactgtacg gaattgctga
     8041 tgattccagt ttttcaagtg attcaaacag aggtgataag gacctcgttg ctgttgtcga
     8101 aaaactagga agtgaggcca gtggaccata tgcaagtctt aaagtcgttg atatacccga
     8161 tggggctgag tgggaaataa gtgattatga cggcgttgaa actgcacatt atggttttca
     8221 aacaggtagt atttagtcca ctaatgacaa ctaaagagga ggcagaagga tgagtgaaga
     8281 tgaatacttt gaacagtagt ctattaacga cagagatttt taactggaaa ggggtgtagt
     8341 catggacgca tcggaagtag taaataaaat taatagtctc aaagaggttt tcggagacgt
     8401 cgaagtagtg ataaacgcac aaggctatga caacggattt tttaagcaga ttaccgacat
     8461 tagcattcag caaggaatgg aagacgaaga tggaaacttt atcgatgaag tagccatttt
     8521 agtgacttgc gagtaagtaa ttgacagcta aagaggaggc agaaggatga aaaaattgca
     8581 ccaactcata tctgagaaag aaagcgaact ccaaaattta gaagatagtt tgggattggg
     8641 gtttccaatt gttgagcaag ctaaaatgac tcaaatcagt catctacgac ttgaattgga
     8701 agatttaaga caaatagaaa aatcaattca actcaacgac aaccagaaaa tcgtgttgga
     8761 gtggttgaaa agcgagacaa ttttaactag agaagcacca atattatctg ttaatgcttt
     8821 ttctgataaa aatttactag gaaaattacc tgataaagta cgcaaagctt ataaactatt
     8881 ggcttgtaaa caagaatatg aagtcctatc agcattcgct caatggggat tagaacagga
     8941 ggaagcggaa tgaacaacag acatcgcaga ataacaaaac taagaaaaca ggaactgaat
     9001 gtactaaaga caaagtttga aaaagaatat ggaatttcag cagaagaaac atataaagtg
     9061 gcaagtcagt ttgttgctga tgcgagtaat gctattcgta agtttgggat ttcgatatta
     9121 aatgatgatc gtaaatggga ggaaatgaga tgaaaataaa agacggattt tacgctagta
     9181 gtcatggtat cggcggttta atgctagaca tgccgataaa gaaccctaaa acacgtcaga
     9241 aatcaaaatt caaaatcggt gacatggtcc gatgtaaagc agaagagttc atctatccgt
     9301 ttcgtggata tgtagagcat gtctataatc attcagcaat cattcacatt gaaaacacga
     9361 tggaatgcga taagtggtta gcgaaaagca aagataattt agcagtggct cgtttggtgg
     9421 atatggaact aatcaatgac aaataaaaaa gccggatcgc tccgactgat tcaataaatc
     9481 caacacattt attatatcac ataaaggagc ggtttgactt gatgcaattg ttacgagagg
     9541 tagatttcaa acagacaaga tgtaatgcga gagatgtgct gaagaacttt cggcgtttgg
     9601 agcggatggc aggtcgctct ttgatagata ttaagtcgcc gattattacg gatatgccga
     9661 aggcaccgaa gcacggcaat aaggcagaag acgcgatcat tcagatgatg gatatagaag
     9721 cggagagaga cgcaatacta gcggctttga tggctcttag tctgattagc cgtcagatac
     9781 tctactacag cttctgtgac gtaaacaagc actctaatta tgaaataggg caattgatac
     9841 gaggatacgg agagaagaat gtagagaagt tgaaatccat cgcattaatc gaatttgcag
     9901 aagcatacaa aaaaggcgtg ttagttcagt atcgttgatt ttgtagggtt tttgtaggga
     9961 tagtgtaggg tttttgagcg gtttaacgtg atattatggt agtgtcgaaa gattagtgat
    10021 aggtctgaga caaaataaaa tgtaagggag gaaatctccc tcatcgtttt aacttaagct
    10081 tcgatagaca gcaacggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
    10141 gttcttatta tctatcatcc gttgttgtct attaatttat gcatcggagg aaaaacgaat
    10201 ggataaagaa atcaaagtaa ctgtcaaatt agatttgact gaactaaaag aactgctcaa
    10261 caaggctagt gaccaagtcg aacagttaca agaaacttta gatgaaattg ctaattttaa
    10321 aatccaagtt tcttagcagt atatttttta gcagctgcac tcataaaatc ataccaagta
    10381 tcagaaaaat ataacttaac taaataatta aaaaaggtgg gtaaatagaa atgacggcgt
    10441 taataatttc aatttttgcg ctctgtctta atgtctatat gattggattt aaaaatggac
    10501 aaaataaaaa atagtagcag ccgagaataa ttttatagtg tcactgtggc ggaaatagaa
    10561 gacgcaaagg tggaggtagg aagcgtatgg cgcactatcg ggaccttgta caaaactaga
    10621 aaggactagt acgtgtgtgg tgcgagccct agggaggaac aggataaccg cctgtgtgta
    10681 ggttgctatt acatattaga tcactctttg agtggtcttt ttatttacat aaaggaggct
    10741 acataatgag aaactactgg tatatatcgc taactaatga atatcctcga accattgatg
    10801 attgttcagt gcgtgttgtg cgttctgtcc aaatcaaaaa gaagtactcc attgttgaaa
    10861 tgaccagaga agctacgcca aaagagattg ataaatgcaa acttctttat tgcggtcatg
    10921 gtttctatga tgaaccaaac attcaaaata atattaacaa gaatttgagg gattagaatg
    10981 caagaaatgg cttaaaaatc tccaatcagg acaaatcaag ttagacaaag tttccgattt
    11041 aaagatattg attgaagctg atttaatgct aaaagatatt gaaaattaga aaacaaaact
    11101 caacctaaga agattgcgag gtggtgtgta ttgaatggca agacaacgtg atccaagacg
    11161 tgatgaagcc aaaagaattt ggttagaatc caacggagaa aagcagttaa aggaaattgc
    11221 atctgaatta aatgtttcag attctcaggt tagaaaatgg aaatcgcaag acaaatggag
    11281 cgctgaattg aaaagtaacg ttaccaatgg caaaagtaac gttactaatc aaggtggcgc
    11341 tcctattggt aatcaaaatg ctaaaggtaa caaaggaaac agcagagcat caccgccaaa
    11401 gagaaataag aatgctctta aaactggtga gtatgaaaca atattttttg acacgttaac
    11461 cgataatgag aaggacatct attctagttt gaatgatgat ccttcttttg ctttgtccga
    11521 agaaatacga cttcttaaaa taagacaact tcgaatgatg aaaagaatcc aacaagctga
    11581 agctggacta aatgatgaag aagtcgaacg attgcagcag ctaagaaaga ttaaaactcc
    11641 gattgaaaaa aatggtaaaa agctagaaat caagcgtgag gttatgcaag atgtgcagat
    11701 tagcagaaaa aaacatcgca aaattgatga tattctttca attgaagatt cattgactcg
    11761 gattagcaac cagttagcta aagccatcaa gcaaatgaat gaactttata tgaatgaata
    11821 cagaactgat ttaattaaag ctcagactga taagatccaa gctgagacaa atgaaattgg
    11881 cggaaataat tcaggtgaag aaatagaaga atggaaacag gcagttttaa atgccgcaaa
    11941 caaacgggcg gtgaaagaaa atgaataatg aatttattcc ttttgctgat attggttctg
    12001 ccattgatta ttactatgat aaaccagtag ctttttgcca agatattttg catttgaatc
    12061 ctgatgaatg gcaagaaaat gttttaaatg atttagctga attttcaaag gtttctgttc
    12121 gttctggtca aggagttgga aaaacagcat tagaagcagg agcaatactt tggttcttaa
    12181 cgtgtcgacc ctacgctaaa gtaatagcaa cagctccgac aatgaagcaa ctttacgatg
    12241 tactttgggc agaggtagct aaatggttaa atgatagctt gatcaaaaac ttactgaagt
    12301 ggacaaagac caaaatttat atggttggtg attcagagcg ttggtttgct acggctagaa
    12361 cagcgactaa accagaaaat atgcaaggtt ttcacgagga ccatatgttg attgtggtag
    12421 atgaagcttc tggtgtgtct gatccaatta tggaagctat tcttggtacg ctatcaggtt
    12481 ttgataataa gctgttgatg tgtggaaacc ctaataatat tgaaggtgtc ttttacgatt
    12541 cccacaattc agaccgtgat aaatacagag ttcataaagt atcaagctat gatagtaaac
    12601 gtacaaacaa agacaatata gaaatgattc ttaaaaaata tggaaaagaa agtgatgttg
    12661 ctcgtgtccg tatttttgga gaatttccca aaggtgcgtt ggattcattt atcagtcttg
    12721 aaacggttga attggctaca gaaaaacaaa ttagtgattc tttagtcaat aaaacaacgg
    12781 ttgctcatat tggtgttgac gtagctcgat atggtgatga ttctacgatt ctctttccta
    12841 gaattgctac cagggcattg gagtatgaga agtattcaaa acgtagcact atggaaacaa
    12901 caggatatgt catcaacatg gccaagaatc taatgagtca atatccgagt attgataaag
    12961 tgatgattaa agtcgatgac actggtgtcg gaggtggtgt aaccgaccgc ctagaagaac
    13021 ttatagaaga caagcattat ccttttgagg tgtttggagt gaataacggt tcaacatcag
    13081 aagacgattt ttacgataat ttaggtactc aactatgggg aaacatcaag gaaatgttag
    13141 aagaaaatat gacagcaaat cttaacggag aacagcctgt tattgaattg ccttctgata
    13201 gttcgttaat caaagaatta agtactcgca aattcaaaat gacaagtaga agtcgtatac
    13261 gtttagaaag taaagatgat atgaaaaagc gaaatattgg tagtcccgat attgctgacg
    13321 cactggcttt agcgttttat gagccaccaa gtcactatca atttattcaa ttttaggagg
    13381 tgagtttttg attagtacag tattatcttt agagagatat aagaagctac gtgttaaata
    13441 tgcaacacaa attgaagatg gaatgtttga tccgaatggt tttatagaat atatgaaacc
    13501 attttttgct gatcgcgaaa gaaaatatct agcttatact agcgaaaaga atgaaataga
    13561 caatagacca aagcctaaca ctgatattgt aaaagttaat aataaacttc atgctggtat
    13621 gtattcaatt gtcgtagacc aagcagtcaa tcattttact ggtataccta tcaaatggga
    13681 ttacgatgta tcggaacaaa aaagaacact cattcaaaga ttaaaagata aattcttaaa
    13741 aaatgatata gaacttccaa cagttccaga agcttttaac aaattaacaa gcaatcttga
    13801 ttccatgaga ttcgcaatgc ttgattctga aactgcaact tttcaaggag cttgtggagt
    13861 agctttccga ttgttagagc ctgtggaaga agatgatggt tggaagttaa gagcaagtaa
    13921 tattgaacct tggagagcag aaagatatgg aaatgccgga atctatatca aagaaaagta
    13981 tgactcttac cagaaaaaat tttttcaaga aatgaaagtt atgacaagaa ataaaattct
    14041 gacatatgct tgctatggtg atttgaattt tgttacaagc ggaacgttca aaaaaattga
    14101 tgagaccgat aaccctttgg ggacgattac cttggcagaa tttaaaaata atacgaatcg
    14161 ttattgtgat tttgaagtag ctgaggaaat tggagatgca attgatcgtg ctttgtctga
    14221 tcaacaaaac gaaatcgaac aatttaagct tgcgtacatg cttgttactg gcacaacaat
    14281 gagcaaaggc actgctaaag aaatgatgaa tcagctaggg attatcaact taaaagaccc
    14341 tactgcaaaa gctgaatatg tcacaaaaaa attagcaaaa gatttcaatg aatatcatat
    14401 ggatctattg aaaaaacagt tttacaccat ttgtaaagca atcgatttta acgatgaggt
    14461 gtttaaatca aacagttctg gagaagctcg caagtggcaa atcattagcc tagaagcgaa
    14521 aacaaatact aaagagcaat attttagaga aggattgaaa gaatgcgcag aaacaattgc
    14581 tgcttttctt aagtttcatg acaaagtaga aattgagcct gaaaaaatta tttttacttt
    14641 ctctagatct ttgccaactg atttaagtta tttagctgaa gctttaccta aacttgcacc
    14701 atacgtatcc aaacgaacta ttcagagcca aattccattt gtaacagatg ttgattatga
    14761 aaatgaaatg atggaattgg aaagtggaag tgcttatcca gatagtgaat acaattttgg
    14821 cggaggtggc aatagtgacg atagaaacga agtattggac caaacgtcga gaactggagg
    14881 atcaagcaag gctcaaacaa gaaaatcaga cacttaaaaa attaactagt gtatttcctg
    14941 aagcactgaa agagatacaa gcaaaactat tatcacaagc tgacttacac aatatcactt
    15001 atccagaaat gatggagttt tacagtacaa gtaatcagaa aaaatatcgt gaatatgtgg
    15061 aaaaaaatta taagtcatta aaaatgtatg atgcaaaata caaagagttt atcgatgaat
    15121 tttttccacc atttgactat gcaaaagtca atcgcttatt acaaatacga tcagatgtat
    15181 ttaaaattct tgcagaatat gcgatggatg cagatgtgaa tcaatatttt tctgatcgct
    15241 tagaggaaat tcttcaaaga acatattctt ctaatgctaa tgtttttgtt caacttttaa
    15301 acgttgatat accaaattat ttaccagaaa atgaaattaa acattatttg aactatccat
    15361 ggtcaggaaa gacattttca agaagacttt ggggaaatat ttcatcgctt gaacagaaac
    15421 tctctaatgc tattgtaaaa agtgttgcta gtggagaagg cgttatacac gcattaaaca
    15481 ccatgagatt agactcagaa atttgtgaca tgtttaagtt agaagaatca aagtataaca
    15541 aagcgataga aaatctcgtt cgaacggagt atgcaaaatt tgcacaagat ggtattgaaa
    15601 aatcatattt agaaacaggt attgaggaat acaacgtatt gactgcaaaa gatgagagag
    15661 tttgccggat ttgtggagga aaggcaagta agaatcccta taaactgaaa gatgctgtca
    15721 taggtgaaaa tcgagcacct ttccatagtc gttgtagatg tacggatgtt cctaatttac
    15781 caaaattagg aaaggatatt gatgaagaat atgaccgttt atttggcgat ttattagatg
    15841 agtttgcaca tgattctttt ggaattaatt tgaaacggag gaagtagaat gaaagatttt
    15901 tttgaagcag tactaacaat taatgtaaat gctgatattg cagaagccta caaaacagct
    15961 attgagtctg agaaccatcc taatggcttg agagaccatt ggaatggcaa ttatgcctac
    16021 gtggttattg gcgatcaaac tgttaattat caagataata ctccagttga taagaatacc
    16081 gttaatttaa cgattcaatt attatctcat tcattaccaa atttaaaaga aacagttgat
    16141 tggtatgaaa agatgggatg tattgttgtt agaactgact acaaagaagg aaagtctagt
    16201 aattaggctt ttttattttg tccgaaatga cgttaaacta gcgcaatact gggcttggtt
    16261 gaatggtggg gcgcaactat taaaactcaa agcaatgcgg ggcgtgaaaa cgaatcgtgg
    16321 ggcgaaagga gaatgagcat gaaacacaaa tcattaatgc caatgaattt gcaatacttt
    16381 gccgaaggtg acgatcaaaa gttttctttt gatgacttca aatcttttgt ggaatccaat
    16441 gaagaagctc aaaaatttat tcagtcacaa tctcaaagtg ttgccgacaa acaattggaa
    16501 gcttggaaac aaaataactt agataaaatt aaacaggata ccatcaagga atatgaggaa
    16561 tctaagaaaa acaagtcacc tgagcaaatt caactggaaa aactacaagc tgaatttgaa
    16621 gcagaaaaag cgttgcgtgt gacaagtgat aataaagcat ttgttgcaga acaaattgct
    16681 ggattagaac tagatggaga gttaaaagag tctatttctc aatttatgct aaataatctt
    16741 gttagttcgg atacagattt cactaagaat gctgttgaag gttttacaag tgttttgaat
    16801 gcaattaatg agaaacatgc agatgcatta aaagaactac aaatgaagtc tgcatttggt
    16861 ggaactcaac aatcgaataa ccaggttcag cagaacaatg aaacattaac aaatccagaa
    16921 gaacaattag gacaaattct tcaacaattt aactaggaga gtgaaaaatt atgaaaaaaa
    16981 catctttaaa taatttagag tatttggata tttcaccagc gattaatgct atgcaagtac
    17041 caaatacacc ttttttaagc tatttacttg gtgctggaaa aacagagcca gcaaactcga
    17101 cagaaattaa atggcgtgaa tatgatatca acaacgatga ttcttctgaa aaacttgagg
    17161 gcggagaata tccagatgct gaatcaggtc gaacttggtt taacaactat actgaaattt
    17221 ttagaaaatc aacctctgta tctggtacat tagatgctat taatgtgaat ggagtcggaa
    17281 atgaattaac taatcaagta gcactacgtg gtatggaaat gaaaattgat ttgaaccgaa
    17341 aattgattac tggtgtaaaa gctgatgaaa atggttctaa aggtcgtcga atgaatggga
    17401 ttttgaactt gatcaattca gcaaataagg cagaaacagc cactgcgggt gcagtaacaa
    17461 gaaaagatat cgatacttta tttaaattga tgtatgaaaa aggttatatg ggagaaaaac
    17521 tatgcttgat ttctccagat atgcaggagt taatgactga tgagttagat ggaaaatcaa
    17581 caaaaattgt tcagttcggg gaaagagtaa cttttggatt gcaaattgga aatatcgtgt
    17641 ctaattacgg tacaggtatt gctctactag aaccatcatt gccaaaagga acaattgccg
    17701 caatcgatac taattatgtg aaactacgtc cattacgtga atggagagca gaagaacttg
    17761 caaaaacaac ggattctaga cgtatcggtc ttgtaggtga atactctctt gaatacaatg
    17821 cttcaaactc tggggcaatt ttaaatttaa agtctgaata aaagggagtt agtactccct
    17881 tttttgatag gaggaattac agtggtaaaa aaagatgaaa ctaaaaaaga tgaagtcgtg
    17941 aaatatagag taggtaaaac taaaaatttt gttgggtttg ttcatcctaa aactcgtaga
    18001 tttatcacag cagattcaaa taacgaattt atcatttcta tagatgataa agaagcaatt
    18061 gcaattttgg aagatgcaat tgatgttaat gaaatttagg aagtgatctg atggatgaat
    18121 cgctaaaaac ggaaatcatt gagtctacaa aagaagattt tccagatttg agtgaagaac
    18181 gcataactaa tttattagaa ataattttgc tagaaattga atcatacaac acttgtaaaa
    18241 atgatatttc atgggaaaag ttaaaaagcg tgattagtga agtgttgtat aaaataataa
    18301 aaaatgaatc agaaaaaaca gtatcttcag ttagacgtgg cgatacaacg attagttatg
    18361 cttcaacaac aaatgatgtt agtgagttac ttctaggcta tggtgatttg atacgaagag
    18421 ttattggttg tggaggattg gagttttttt aatgaatgaa gcagatattt tagaaactac
    18481 ttatgaagat agttgtatta ttgaaagact gacggacatt gaagattcta atacaaatat
    18541 tactattcaa gattacaaaa aagtatatga taatcctatt tcttgcgctc tttcacaagg
    18601 tcaaatcgat ggactagcag tcatagaaga tggagagatg gtaaatgttt caactgacac
    18661 atataaatta tttgttcatc ctaagattaa actaaaaaaa ggagatcgaa taacaataac
    18721 tcaaaaggcc agtggcttaa ttttttctct atttgctact aagccttttt actatcctag
    18781 tcattgtgaa gtgaatttga taggaagtga gaaaaatggg taatctcaaa tatgaatcaa
    18841 atgcagaaaa gatcattgag aattttaaaa atatgactgt aattgctcaa aaagaaggaa
    18901 tatccttcgt taacgattca atgaataaag tcgttagttt agctaaacca ttaactcctg
    18961 taaaatcagg taatttaagg cgaggatatc gtgtagtaaa agctagaaaa ctatcaagcg
    19021 gtcgaattgt tggagcagtt ataaacaatg aacattattt taaatatgta gaagaaggtc
    19081 ataggactaa aaatggtggc tttgtcaaag ggaaattcat gttaactcgt gcaacgaatc
    19141 ttgcaaatat gtcttatatt cctcgaagat ttaaacaaat ggcaataaaa atcgttaaga
    19201 aaggaaagta acatgaaaga tgaaatcatt gctgcaatca gtagcaaatt aaaagaaatc
    19261 tatcctgatg ggacaatata tcttgattca gttatgcagt caactaaaga tttttatttc
    19321 gttttatcag taatggaatc tggaactgaa aatgtaggaa ttgatattca aaatgtttct
    19381 ttcttaattg atattgcatt gattgataat aaacctaata gaaatttaat taatgaatta
    19441 gtctcacgtt gtgggacttt ttttaatacg attacaatcg atgagcaaat attatttcca
    19501 aaagcctatt tacctgatga agcagatggt gttcaacata ttcgttttac attagaattt
    19561 ccacaatata ttgaatggag tgaaagataa atgggagaaa aaagaagtaa aaccggaatt
    19621 atttctgtcg aaaaacctac ttggtttcct ttggaagatg agacagggaa ttttccggta
    19681 tatggtactg cgttgccaat gggaacagca gtaagtatca aaccaacagc taattatgaa
    19741 acaacacaag attatgggga ttcagtggtt caagatcaat ttacagcttt tggtggtgca
    19801 gaagttgaac tggaagcaaa tggatatact cataaagttt tatctacaat tactggaggg
    19861 aaaattgtta aaggtggtgc cttgcgttct ggagaagata ttgcgcaaga tggtgcgttt
    19921 gcatatagac gtaaaaaatc gaatgggaaa taccgttata cggtctttta taaaggacag
    19981 tttgctctag attcagatga aacatcaacg attgaaggaa gtaaagtaag ttttactcat
    20041 ccaacttgga agggttcatt tgtagatgta ccaggacttg gatatatgta ttcagttgat
    20101 gaagatgatg aaggcgtgga taaagcaatg atcggaaatt ggtttacaaa agtagcaatt
    20161 ccaattgaag aagcagaact ttcaggaggt acagaataat gtcaaaatat caaacaacga
    20221 ttaaattaac gaaaaaagat gaagaaggta aatatgagca agtacaattt aaatctgctg
    20281 aatttctacc aggcccagtc gtagaagaag cagcagaagt aatggaggga atgcaaaatg
    20341 ctgtagatgg aaaatcagta tcagaagcat taagtcgagc ttattcattt attgcagata
    20401 ttttgtttga aggacagttt actggtgaag attatcgtca agggatcgat gctcgagaaa
    20461 ttgcttcgtt gacaggtaag ttattaaaat ctgttactgc aggctttgat gaaacttata
    20521 cagaaacgaa aaaaaagtaa gcgaagctct caaatatcct ttttttaaac attcgattac
    20581 ttatcgagaa ttagatttaa agacgcaatt actcgaagca ggttggacgt taccagaaat
    20641 tgaacgtact gactttgacg agttaatgcg tctttttgct tttcgagatg cagtaaaaga
    20701 gcatgaggat gttgagtact acgataactt cacccaattt taggaggtga tactttgaat
    20761 aacgatgacc taattctgaa gatgatacta gatgaatctg gttttactgc cggtatgaat
    20821 aatgctgtca aaaagttgaa ctcttttgat gaaacaatcg aaagaaaaag tagaaatagt
    20881 ggcagttcat taggtagtat ttggaaaata tttgctggaa gttttctagc tagcggagta
    20941 actagaatcg taggagctgg ctttgatcta atcaagggtt ccataagtgg agcggttgat
    21001 cgagtagata cgatgaataa cgctctacga aatttccaaa atatgggatt tagtaattca
    21061 gaaattatga agaatatcgg aaagaatggg cttttatctc aaggtattca aggacttcct
    21121 actgctttga atgatgcgat aagtcatgtt caacttcttg cttcttctac aggtgattta
    21181 actcgttcaa ctcaaatatt taaagcttta aatgatggaa ttcttggttt cggtggttca
    21241 actgatcaag ttaatgaagc tgttattcaa ttatctcaaa gtttctcaaa cggaaaagta
    21301 gacgcacaaa cttggaactc aatgatcaac gctcaacttg gacctactct ttctgctatt
    21361 gctaaaaaga tgggaattac aatgggagat ctgaaagaag gtttatctca aggtaagatt
    21421 tctgttgaag aattccaaaa tcaattaata gaaatggata ccaaaggtgg cggaggactt
    21481 aaatcattaa gtcaaatcgc taaagattct actaagggaa ttaaaacctc tatacaaaat
    21541 gcaaaaacag ctgttacacg tggtgttggt gaggtaatag aaggattaaa taaagcatta
    21601 gtagattcag atttaggtgg atttaaggga attattgata aagtgggcag ttcaatggaa
    21661 tcgttcttaa aagtaattgc tgcaaatatt cctacagcag tatcattttt aagtaattta
    21721 tttaacgaaa ttcaaaaatt tggttctgct ttgaaattca tgatgccctt tcttgttcct
    21781 gcagctaccg cttttggagc gttcatgttt caacttaaag gtataccagc aataataaaa
    21841 agcttcaata attttaagaa tgccataatt ggcgttggaa attcattaaa gattatgggg
    21901 gcaatagctg ctgctaattc atttgtttta attattggtg cagtcgttgg tgcaatagct
    21961 gttttcggtt actttatggc aaccaatgaa gagcttagaa acaaggttat atctatttgg
    22021 aatgatgtga aagattccgt aattggtgta ttaaaaaata taaaggactg gggaattgat
    22081 acttggaatt ctgctaaata aatggcatca aatgcagttg agggtgtcaa agatgcttgg
    22141 tcaggaacaa aggaatggtt ttctaacacc tggaaaggta ttaaggatgg ggccacaggt
    22201 ttatttgata aaacagtaga gacttcaaga aatgcagtcg atagtgtaaa aaatgcatgg
    22261 tctaatatga agaaatggtt ttctgatact tggcaaagta taaaagattc agccagagaa
    22321 aaatggaatg gaatcaaagg ttctattatg tcagttgctg gtccattgat tacaggtata
    22381 aagaatgcat ttttacatgt tactttttat ctagagacct tatggaataa tttagttgag
    22441 attggtaaaa acgtctttga gattttaaaa aatgttatac ttgcgccggt tttgtttatt
    22501 acctccatga tttctggtgg atgggaagaa acaaaaaata atatgattgc tgtttggaat
    22561 aatattaaag aaagtgccat aaatatttgg gaatctataa agaatatatt tgtaagttat
    22621 tttacaaata tttattttgc tgcactcaat atttggacag ggttcaagct tacattgata
    22681 aatatttgga atgaagtggt aaatcaagct aaa
//
LOCUS       DAYYGS010000047        22504 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000047 DAYYGS010000000
VERSION     DAYYGS010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.047"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(22..219)
                     /locus_tag="ACHA44_001669"
     CDS             complement(22..219)
                     /locus_tag="ACHA44_001669"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8904944.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
     gene            complement(365..1243)
                     /locus_tag="ACHA44_001670"
     CDS             complement(365..1243)
                     /locus_tag="ACHA44_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8904945.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1282..2400)
                     /locus_tag="ACHA44_001671"
     CDS             complement(1282..2400)
                     /locus_tag="ACHA44_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8904946.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2397..4496)
                     /locus_tag="ACHA44_001672"
     CDS             complement(2397..4496)
                     /locus_tag="ACHA44_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HFM8904947.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4547..5368)
                     /locus_tag="ACHA44_001673"
     CDS             complement(4547..5368)
                     /locus_tag="ACHA44_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8904948.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5365..6174)
                     /locus_tag="ACHA44_001674"
     CDS             complement(5365..6174)
                     /locus_tag="ACHA44_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8904949.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6195..6689)
                     /locus_tag="ACHA44_001675"
     CDS             complement(6195..6689)
                     /locus_tag="ACHA44_001675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8904950.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6701..7201)
                     /locus_tag="ACHA44_001676"
     CDS             complement(6701..7201)
                     /locus_tag="ACHA44_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8904951.1"
                     /translation="MIKPMFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLS
                     SIQLLTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITK
                     TFLEKEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVN
                     EEEDFF"
     gene            complement(7266..9962)
                     /locus_tag="ACHA44_001677"
                     /pseudo
     CDS             complement(7266..9962)
                     /locus_tag="ACHA44_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(10390..11463)
                     /locus_tag="ACHA44_001678"
     CDS             complement(10390..11463)
                     /locus_tag="ACHA44_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8904952.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11460..12287)
                     /locus_tag="ACHA44_001679"
     CDS             complement(11460..12287)
                     /locus_tag="ACHA44_001679"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904953.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12288..13094)
                     /locus_tag="ACHA44_001680"
     CDS             complement(12288..13094)
                     /locus_tag="ACHA44_001680"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904954.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13106..14191)
                     /locus_tag="ACHA44_001681"
                     /pseudo
     CDS             complement(13106..14191)
                     /locus_tag="ACHA44_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(14208..14750)
                     /locus_tag="ACHA44_001682"
     CDS             complement(14208..14750)
                     /locus_tag="ACHA44_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFM8904955.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15008..15997
                     /locus_tag="ACHA44_001683"
     CDS             15008..15997
                     /locus_tag="ACHA44_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8904956.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            15994..16731
                     /locus_tag="ACHA44_001684"
     CDS             15994..16731
                     /locus_tag="ACHA44_001684"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HFM8904957.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            16728..17279
                     /gene="coaC"
                     /locus_tag="ACHA44_001685"
     CDS             16728..17279
                     /gene="coaC"
                     /locus_tag="ACHA44_001685"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HFM8904958.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17295..17927
                     /locus_tag="ACHA44_001686"
     CDS             17295..17927
                     /locus_tag="ACHA44_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFM8904959.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18012..18659
                     /locus_tag="ACHA44_001687"
     CDS             18012..18659
                     /locus_tag="ACHA44_001687"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HFM8904960.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18664..19647
                     /locus_tag="ACHA44_001688"
     CDS             18664..19647
                     /locus_tag="ACHA44_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HFM8904961.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(20050..21564)
                     /locus_tag="ACHA44_001689"
     CDS             complement(20050..21564)
                     /locus_tag="ACHA44_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HFM8904962.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            21702..>22504
                     /locus_tag="ACHA44_001690"
     CDS             21702..>22504
                     /locus_tag="ACHA44_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904963.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
BASE COUNT         6791 a         4831 c         3905 g         6977 t
ORIGIN      
        1 ttatacggct gaaaaaatct atcaccaaaa aaattcaaac attaagccaa ggattatgaa
       61 gtcaacattt cgaaaaatga acatacattg gtaagcaact ataactatta ccacagacat
      121 aaagtaacac aatatattac tatttaagtg tacgccacca gtgaatatcc tcaaggtata
      181 cagcgcacaa caaaaagaaa aaaggaaacg cagaagcatt tcctttcagt gatttaatga
      241 aaacaacacg atttgactta gagccttttt atttgaattc attttcgctt tatccaactt
      301 catttgatca tttgattact cggttaaata cttttgacat gagtatcaat gaaacattca
      361 ttaattatgg ttgcttattt cttaccgctt ttctggccaa tgctaatccg ccgattattc
      421 cggcattatc acctaattcg ggcgtaataa tgtagctatc aattggtggt atttcaacat
      481 atccatttat aagctcaaca aatttatcct gaactttttt cttcatgtgt tcttgtttca
      541 ttacaccacc gccaaaaata attctatctg gcgaaaacat cagtgttgta ttgtatgcac
      601 attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc gtcagccaat aaattctggc
      661 cttttacacc caaccgtttt tcaatcgctg gtcctgctgc catcccctct aaacaatctt
      721 ggtggaaggg acagtttcct gaaaaattgt cctttggatg tcgcttgatc aacatatgcc
      781 ccatttccgg atgactgaac ccttccacga aagtcccatt gaacaaggca ccacctccga
      841 tacctgttcc cacagtgaag tatgcaacac ctgctaaccc tttgccagaa ccagcaacat
      901 attcaccata gcaagccgca ttgacatctg ttgtccattc aatcggaata ttgaaatatt
      961 gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag ttttggtgtt gatgtgatat
     1021 gaccgaaact cgttgatttg cattgaatat caattggacc aaagcagccg accccgatgc
     1081 ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac ctgcttcatt gtttcaactg
     1141 gagttgttgt gggaaaactg actctttcca aaatcgttaa ttcctcagta ccgactgcac
     1201 agacaaattt agtccctcca gcttcaattg ccccatatat catcataggt tcctcccttc
     1261 gaatatgtga aaaacacttc attaacgcgg caagcgttct cgatcagtga aatgacgttt
     1321 agtatttcct gaagcttctg ttgaatacca gtaagcgaca ctagcaacat cgtcttgccg
     1381 ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa gtgaccgata aatcttcggt
     1441 aaaacagatg gggtcttgaa gatgccaacg ataaagacca tgtcgtggca ttgcgtcgtc
     1501 accaaaggca tgaacggcat tcaattttcc cgtttcaaaa cgatctcttg tggcatctct
     1561 atttgattgg taaggatacc ctaaaaaggc tgttgaatag tttttcgcat gtggacgtcc
     1621 gaactcatcg tactcatgaa aggcccacgc cccgccaaaa taatcttctg aacctgttga
     1681 ggttacggtc ggatactcac tatccccatc aatgtagaat ttaaactcgc cttcgcccca
     1741 ccaataacgt tctaggcagg taagcgcaag gtaagtaccc acgtaatagc cttgaccctt
     1801 taatcgatca attaacttat aatcctcctg taatttggta atgcgttccc gatgccaatt
     1861 tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc aaagcgtaat ttatcgtata
     1921 aaaaaatgag gacatatcct tcggatgtaa attctcgatc gtaattttag cggatgtttg
     1981 gaaaggcatt tcgaaatagg aattaaagcc ccctgtagga ttaaccacta tcggcaacga
     2041 gttcacctcg caacgctctc caaacccatt acaaaagaaa tcccctaaag gactttcaac
     2101 agcagggcta tccgacccat cccaataaat tcttaagatt aaatcacgta aaacaaaact
     2161 gccaaattct gttcgatcac gaatcgtcat ccacatatga cgaattattc ccgttccagt
     2221 aatatccgca atcacagttt cttcaccaga agtcagcgga attgcgccac gacctttgcg
     2281 gcttggtcct agctcgcttg gcaacatcgc agctttacct ttctcgcctg ttggattttc
     2341 gggagtgatt gctcgactct ggattttttt aaattgggta atatttttta gtatactcat
     2401 ctttttctcc tttgaatgtg aatcgttttt cccttttcta cgtgaagaat ttttttcgta
     2461 tcacttattt tcaatggctt ttctgacaaa tttgaaatac tcaactcgga ttgcgtacat
     2521 tcaatcgaaa tgaaggcttg accgaacttc aaattcttcc aagaaaacgt taaattatca
     2581 acaggatcaa tttgcaatac tccttttttg aatttcaatc cgaaatattg actgatcatt
     2641 aggctgacaa aaagacccga tgcccagccg catttgccgc agccaaagtc tcgcacgact
     2701 tctccttgtt gagcctctaa gcgatacggc caccaccacc acgaaccgtc taaatctgcc
     2761 aaacaaatta gttcttctaa tcgggttacg aaagcttgag ggttgtgcag ctcgctcatt
     2821 aaaacagtga taaagccagg aaaagtggcg ccggattcta atccccaagt gatgcctttg
     2881 atttcctcgc tataggttgg attgaaagaa ctagcagaaa aggccatggt gttctgatat
     2941 aaatgatctt tattatctaa aaattgataa aaaggcatca acgtagtgtc tgattcttct
     3001 ccatcatgca tcaataaatt aatttttcca tccacaatca catcacttaa aaagattaac
     3061 ccttgctcaa gaatcggttt ttgatagtgc ttcactgaat aggttgtttt ctcctcatca
     3121 ccgattccct ccaaaaattg ctcaccaaaa ggaccatcga tagtcatttg tttacaaatt
     3181 gattccttta gtttacaggc aatgtttttg tatcgcttgc taagactcag ttgttctagg
     3241 gcttcaaaaa tttctgcaag tccaatacag gccttccaaa gacaaagatt gctgccggta
     3301 tgatactttc caagtgcaaa ggcgtctgat aaccaaacag atggaaaaag cattggttcc
     3361 cctgaccgac gttgagctaa tatgtcctca ataattcttg aagcattcag tagaacctgc
     3421 ggacaccgct caaagaattt tgtatcccca gtatgttcaa aataaagact agccagtaac
     3481 gcactggata acgaattact cagagaatga ttgatacctc ctgtaaattt tgaaccagga
     3541 aacttaacgc catattgatc aaaccataga accgtttttt gacataactc cggttcaaac
     3601 atggtgaatg gcaatgatgc gtaatacata tccttattcc aaattcgatt agtagcagga
     3661 tagctgcccc aattgctgcc gacgacctgt ccctgtcgat tcataccgaa actagaaaag
     3721 ctttgataaa gtgcacgacg atagagatgg acaactctat cgtttggcat tgacagctgc
     3781 ccaaatagct gagcaaaata gttcaatgtt tccttcagcc atttttcgat atcagattcc
     3841 tgaaaagtca ttacttcttc aaaagcattt gggtccacaa aagcaatggc gaagcttgcc
     3901 acttcctgtt gtgcgatcgt gtccttattt cctttttgag aaatcaacac attttgctga
     3961 tccgaatact tctcttgata cgaaggcaca tctttcactg aaacttgaag gctttcgtgt
     4021 gtttcattca caatgtaaac ctgttgaaca agcatactta aacgcttatt agccagtatg
     4081 ggagcaaagg atactacaat gaccttaaac gtcgaataat gatgaatata tctaggcaaa
     4141 cagtcttgaa tcacatcgac ttcaatacga tgatccgatt ctgacaaatg gtagacaatc
     4201 ccgtttactt caacctttaa ggataacgaa ttggtttgag agagctgttt attgacccaa
     4261 acacctggtt tttcctcagt tagtaaattt tctcgataaa aggtcaacgt ttgaatcgtc
     4321 ccgcaaacat caaggtttgc taatgtcttt tgattactta catcgaaggt caatcccttt
     4381 acatcaggaa tattccgata gtagctgtat tcgtttgaag tagtcacatt tccgtcgttt
     4441 aactcagcat taaaaaatgg gtgtttgggt gaataaatta cttcattaat agtcatcttt
     4501 tccctccttg ctaaaaaaac tgcagcctag tagctgcagc ttcctactat gcgattccaa
     4561 gtaatgacaa taaaattccc aagacgacca ttccaaaaat aattttgatg acgccgactt
     4621 ttttatctag taatttgaaa cagctcaatg ttaccaataa cggtacaatt cctttaaaaa
     4681 tactatcgag gatttcttgg acattcatta gttcttcgcc cttcattgaa aagctcaaaa
     4741 cggtattgaa tttgaccata ctgatcgtca ttgcacctac catgatcaga cccaagatac
     4801 tagcagcttt tgtcagaatt tcaattaacc cgttcgcata cagcttttca atatatttag
     4861 agcctaatga atagcctaaa acgccgccat agtatcgcat caagtaatta ggtatattga
     4921 acataactaa aaagataatt ggtgccaaaa tatttccggc attggctaag ttgatcgcta
     4981 gtcccgcggt aacgacacgc caaactcccc agaacaatga atcgccaatt ccagccaatg
     5041 gtcccatcaa actcgttttg atcgcgttga tactttccac gtcaaaacca gctttttcag
     5101 aattttcctt ctccatcgat gccgcaatcc ccatgacaaa ggacgcgcaa ggaatggtcg
     5161 tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg atcttcttca cttttgtaga
     5221 aatgattaat gaagggcatc atagaatagg cgaaaccacc gccgcccatc gtcaccggat
     5281 tcaccgaagc atacatagtc atcgagcgcc aaaacatctt ccgaaccaat tttttctcat
     5341 ctttagaaat tccatcagct aatttcattg aaagaaatcc tcctcttctt ggtcaagcgt
     5401 tgcatttaca gcagcatcgc tttgaactgc atgaacagat atatttttta gttgtagatc
     5461 tctttgggct ataacaattg caataatcgt tccaactgcg gcaattgcaa tcaatggtaa
     5521 ctcgagatac gccataacaa cggccccaaa gaaaaaccac acagcaatat ttttactcca
     5581 aagcatccgt aacagcatcg ccatcccaac tgccggcagc agatttccag cgactgttaa
     5641 tccgttcatg atgacctctg gaatattatc caagacgctt ttgacaacat ctgctccaaa
     5701 taaaacggca aagaatggaa taagagcata aagcgcatag tggacaaacc acataaagaa
     5761 gtgcagattg atgattcctt tttgatcacc agattttgca aatttgaaaa atttagcagc
     5821 catcggtcca catacaaaag aaaaaatcat attcattaac tgtaatccta atactgctag
     5881 tggaaagaca atgggaataa tcgcatccac gtttcctccc attttgattg cgaacgctgt
     5941 agccagccca gcagccaaag aaggctccgc cgtactagag attccgatat tgacgacacc
     6001 cataaagatg gcttctagtg acgccccgac aatcaacccc gtctgcaaat cccccatcaa
     6061 cgctcctgct aacggagcaa caacaatcgg acgactcagc atggacatcc cgaacatttc
     6121 atgtccagca actccaataa aaacggccaa agcgactaca aatgcatcat acataatttt
     6181 tctccttcct ttttctaaaa atcactcaaa atagagatag ctgaacgttt ttccatcgcc
     6241 ggtgtaggct gaaaaacggt atctggataa agttcaacta attcttttaa gtcttgaatt
     6301 tctgacgtcg ttaacatcac ttcctttgat aaggttttct tatcttccga tggcggtgtc
     6361 attttcccag cattccccac gttcattaat tcgatttccg ttgggaaagc tttcgcaact
     6421 tttaccgcat ctttgacatt agggactaaa accatcaagc gcatgtcctt cgcccgcgga
     6481 tcttttaaca aatgaatcgc gtcatcaact gttttaataa tgactttgac attcgatggt
     6541 gcagccatct tcaatgtcat cttttgaata tcgttggtag ccgcctcatc gcttgctact
     6601 actaacccgg ttaaatttag actctttgtc caagtcatcg ctacttgtcc atgaattaat
     6661 cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc ctaaaaaaaa tcttcctctt
     6721 catttactac atgtacctca tcaagaatgg tcatctttaa ttcctgttgg catttattta
     6781 ccagttcctg aatatcctcc tttgttaatt ccttggttgc caatactatt tcgagcagaa
     6841 ttggcagatt aaagccagac aaaatagtta cagcaatctc tttctccaaa aaggttttgg
     6901 taatcttctg attgacactt ccgccaaata aatcggtaaa aacaaaaatc tgatcctgct
     6961 cgttcacctg gtttacaaat acattcaact cctgttcaaa atcacactca tccacataag
     7021 cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa ttgaatactg ctaagaaagc
     7081 ctgaagctaa gtgaccgtga gtcgctatta gaattttctt catatggtta attcctttct
     7141 ttctgttcac ttttatataa gcaattcctg tgccaacttt taaaaacatt ggtttaatca
     7201 cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa cagattagac acttttttag
     7261 acactttatt tttgaatata cggtcctaat aattcataga gcagcgaaag ctcataagca
     7321 ttgactttaa aattatattt catctccatt gattgaaaaa tacttctaga aacataataa
     7381 aattcatctt catctggttt caaatcataa ttcggttctt cagatttttc tgtccgagcc
     7441 aagaataaac gttccatcat caatgccgta tgcatataga gatttagttt tacacggcca
     7501 tttaaagtga aatgatagta attttcatat ttagaaatga ctgtttccac ttcactcatc
     7561 acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc cttccaacga aaagaatttt
     7621 actagctcct gaatcaagcg gttaaattct gccggcttca tttcaggact caaaatatgc
     7681 tccaaattgg ctgctccatc accgtctaag atgtcataaa cattaatatt aggaattgaa
     7741 aaggaacgtg gaagatccga agtcgtgatg accaattttg tcttgtcaaa ataagtttca
     7801 tcattttggt cgattaattc acgtagttct ctgtagtcct tcgtgaaaac ttcgatggat
     7861 tcatccgata aatgatgacg aaaaacatct ttcaatttgt ccgaaatccc caaacccgac
     7921 atacaggaaa tgataatgtt gctactttga gaaaagcctt caaagtattg aacattgatt
     7981 gaatacttgt ctgctgcatc ctccgcaatc tatttaaatg gcgcattcat cgtcatcttc
     8041 aagccaatat caagtgcaat ggaagtcgtc aaattattca gaagaagtaa ctcccctttt
     8101 aaatcgttct tgatctgtgt gtaaatttga tttaaagagc ccatatctac gatcagcact
     8161 agactatctg tattcacttg ccgtttcacg aaattccttg tcgattcgac aatctctgac
     8221 acattactct ctactggcat atccaacgct tcaaaaacat aattgccgca taattgattc
     8281 actactgctt gaatgctgct agccgtacta tctccgtgag caagcaacag gcccttcagc
     8341 tcgatggatt catctacata atcagataaa gtcacgacaa taagcagtct tagaaagttc
     8401 tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact gattacataa gtaagtcgtc
     8461 cgaggtagat ttgcagctaa tgtacgattt atcccggtta agtctgtctt atagcttggg
     8521 gaaatgacat aggatctgct aataatttct gcaacaggtc cactcgtcgt caatccgtac
     8581 gtttttccta aaacggatac ccagaccttt ctaaacatct gaatcatcaa ttttctactc
     8641 attgataaaa aagaatcctt atcaatgtga tgcaccaaat gctgtacttc attctttatc
     8701 gaagaaaaat tttcattgaa attaccttca tttatcagct gagtaatttt ttccaaaatc
     8761 gaatcataat caaacggttt gattcgatcc gtttttatct tttgattttt ctttatcaat
     8821 aaactagcaa tcggcaagct ttctacttcg gattccagca tatttaactg aatcaagatc
     8881 tcatttttcc caatcgttaa tggttcttct tgtgttcgac tatacacatc cgcacaactg
     8941 attttaatga tattcttcag cttaccaata ttcccactaa agggatgaga taacattaaa
     9001 gacaaggctt ctcttgaaac aataatatct ttttgtaaaa catccgcttc attttgaaaa
     9061 aataattgaa tcagctctaa tcgttctcga atggggcgat cttcaaaagt tggcaacata
     9121 acactaattt gaatacgccg atcaaaggtg tctaataaat aattttcacc tttttccgtc
     9181 gtggcgaaaa taaaacgaac cttagcagaa tgccaattta cattttcacc gactggacga
     9241 tagtttccag tatcaataaa tagaaagagc ttttcttgat tttctttcga taggcgatgg
     9301 acctcatcga gaaaaagata gccgttgttg gccgttgcca ataaaccatc ggtatccttt
     9361 tcagcccccg taaaggcccc cttcttgtat ccaaacagtg ttgccgaaag cagttcggga
     9421 ttgtccgcat aatcggcaca gtttaagata gcaaatggtg catctttttc aataatttgc
     9481 tcatggatag cgtattcaaa aatttttgtg gctaaaaaac tttttccaac acccgtggct
     9541 ccatttatta atacactgag tccgttaggc ggatatttga cagcggctat acatttttga
     9601 atgacctctc tcagacttcc gtcatgacca actacagaat gaaaggcttc gtttatttgc
     9661 tccgtattat ctacaaccgc atattcatct aaaggcttcc acaaaacagg ctttcccggt
     9721 aatttctcga cctttttatc ttccactaat ctcgtcaaat aatggctggc attctgacga
     9781 gagataccta gattcttcga aattttctcg gcagtgacag aagacatatt ccctgttagt
     9841 cgtatcagct catcaaaaac tcgatcttta gcgctctttt tcattttttc ctccagttta
     9901 atgtgtctaa aagcataata aactatcggc tgcaatatgt ctattctata tttgtattcc
     9961 aatttttact ttgaaacaca aatagtcgaa tatttgcctc agatatctga caaaataatt
    10021 tatctaccaa tttagttttt atctaatatg atttttatgt tttttcatag ctacaaatat
    10081 tgaactggat tcatatatta gttttattaa tagattacag aagggttaag tatatttgca
    10141 tagtttccaa ttactttatc cttctgtaac tttttcaatc ttagatttac tagcccgtct
    10201 cgggccctat tttacaatgg ttttgtccac ttttctctgg tagaaaaacg tggtagaaaa
    10261 aataaaataa cacaaaaaaa gcggcgtttc atccgctttt ggtagaagat tggaaaactc
    10321 agatagaaaa aggacgattt ttagctcttt agaacgttga taagtcccca ttcttactga
    10381 gttttgccat tatctcctat acatcttgaa ttctaagaat aggtcattat agatgcctag
    10441 gtatttcgat cctagatctt tgtagacttc aagatgggag atcgtgtcat cagatggata
    10501 gaattgttcg tccgacgaaa tgtcttttgg caataacgct tttgcttctt catttggtgt
    10561 agaataacca atatactctg cattttgtgc cgcattttcc ggttctagca taaagttgat
    10621 gaaatcatag gcaccttctt tatttttcac ggtttttggc ataacgatat tatcaaacca
    10681 aagattggat ccttctggtg ggatcacata atgtaaatgt tcattttccc acatcatatc
    10741 ggcagcttcg ccagaaaatg tcactgccac ggaggcttct tcattgatca tatacatttt
    10801 gatctcatct gccacgattg ctttgacatt tggtgttaaa tgattcaatt tatttgctgc
    10861 ttctgtcagc tgtgccatat tcttactatt caaagaatag cctaaactat tcaaagaaag
    10921 ccccataact tctcgtgcac cgtcaatcag catcaaactg tcttttaatt ccggcttcca
    10981 caaatcatcc caatgcttaa tttgatttgc atcaaagaat ttatcgttat atacgatccc
    11041 taaagttccc caaaaatatg gaatcgaata ctgattatct ggatcaaaat ccaagtctaa
    11101 aaatcgtgga tcaatattat ttaatccttt gatcttcgaa tgatctaatg gaagtactag
    11161 tttttctttg atcatttttt ggatcatata ttcacttggt atagcgatgt cgtagtttgt
    11221 cccaccttgc tggatttttg tgaacatcgc ttcattagaa tcaaatgttt cataattcac
    11281 tttgtatcca tattcttttt caaatttcgt gatcaaacta ggatcgatat aatctcccca
    11341 gttatagatg gtaagtacct ttgctccact catgccactt gttttttcaa gttgacgaac
    11401 accaagcagc agcaccgcaa taatgatgag aatacctaaa aacagagatt gtaatttttt
    11461 catcgcagcc gtgccacccc ctcgcgtact cttctgtttt tcttcagccg ttttggacgg
    11521 ttttctttac taataaagta gtatccaatg accaatatca tggagaacaa gaaaactaac
    11581 gcacttagcg cattgatttc taaactgatt ccttgtcttg cacgagaata gatctctact
    11641 gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa ccgcaaaatc atcaagagaa
    11701 taggtaaatg ccataaaata tccagctatg attcctggag aaagataagg caaaatgata
    11761 tttttgacta cttggaaatt attagcacca aggtctctag ccgcatcgac cattgaatcg
    11821 ttcatttcct gcaattttgg caagaccatc aagacgacga ttggaatgct gaaagcaata
    11881 tgggaaagca atacactctg aaaacctaat ccgaatccta gtagtgtaaa gaaaatcaag
    11941 aaactagcac cgataataac atcaggagag actaataaga tgttattcat gctcaacaac
    12001 gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca tagccccaaa tgtcccgatg
    12061 atcgtggcga tcaatgcaga taagaaagcc agcatgaacg tattcaatac gataatgatc
    12121 aaacgtgtat cttcaaaaac agcttcataa ttttcccaag taaatcctgt aaagctgttc
    12181 atcgttcctc cttcattgaa ggaataaaaa atcagataga agatcggtgc atataggaat
    12241 aaaaagacga tccctaaata aagatacgac catttaaatt ttttcattta tgagcgacct
    12301 ccttttttct tctcgcctgt caggagcatc acgatgaaca tagcaatgat taaaatcaca
    12361 ccgattgtag aacccatccc ccagttttgt gtaaccaaga aatgctgttc gatagccgtt
    12421 cctaaagtga tcacccgatt tccgccaatc agacgagtca gcatgaataa agaaagagaa
    12481 ggaataaata ctgcttggac accacttttc acaccattta atgataacgg aaaaatcact
    12541 cgtcggaagg tctcaaagct attcgcgcct aaatctctac tagcactgat caaagaagga
    12601 ttcatctctt ccaatgcatt gaaaattggc ataatcataa atggaatctc gatatacgtt
    12661 gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt tcggcccgat cccgataaaa
    12721 tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc caataaatgc gtaagcttta
    12781 agcagcagat tcacccatgt aggcaaaata atcagcatca gccataattg cttgtgcttc
    12841 agttttgtta aaaaataagc agtcgggtaa ctgatgatca gcgtgaaaaa cgtgatcaaa
    12901 aatgcatacc atacagaatt gatcgtcata gaaagcgatg tccccgacgt aaaataagat
    12961 agataattat tcagcgtgaa ttgcccattc atatcaaaaa aagattgata aatgatcatt
    13021 aatacaggcg cgatcacgaa taaaccgagc cacaaaatat aagggacgga atagatcctg
    13081 cgcattgttt tcattcttgt cccccctatt cttcgtagct gtcaagacgt gcatcgaatt
    13141 cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc tggctcaaag gccaatccta
    13201 ctttgcttcc ttctttagct tttttcgttg aatggaccat ccattcattt tgctgttcat
    13261 cataacagat gatttcataa tggacaccac gaaacagctg agtatcgacc gttacgacta
    13321 gttttccttt atctggtgtc gtgatcgtca aatcctctgg gcgtaaaaca acttcaactg
    13381 gttcatttag acgcatcccg ccatcgacac attcaaattg cttagcaaca aactcaacta
    13441 gattatcttc gatcattaca ccgtccacga tattgctctc cccaacaaaa tcagcaacga
    13501 aatgattgat tggctcatca taaatatcta cgggtgtacc actttggacg attttccctt
    13561 tattcatgac aaaaatctca tcactcattg ccaacgcttc ttcttggtca tgagtaacaa
    13621 aaataaacgt gattcctaat cgttgttgca gatcccgcag ttcatactgc atatctgtac
    13681 gtagtttcaa atcaagcgcc gataacggtt catctaatag caatactttc ggttcattta
    13741 caattgctcg tgcaatcgct acacgctgtc tttgaccacc agacatttca ctaatctccc
    13801 gtttttcgta tccaggaagc tggaccatac gcaaagcatc ttttaccttc ttttcgatat
    13861 ctgatttgga cattttcttg atcttcaatc caaaagcaac gttatcaaaa acattcatat
    13921 gaggaaaaag tgcataatct tggaagactg tatttacttg tcgcttatta gctggcaaat
    13981 cattctaccg tttcccttca aaatagatgt ccccttctgt agcttcggtg aatccagcta
    14041 taatacgtag gatcgttgtt ttaccacagc cggaaggacc aagcagtgta taaaattttc
    14101 cttgttcgat ctcaaaactt acttttttta atactgtttc atcatcatag cgtttcacta
    14161 cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc ccctatatta caaatatgat
    14221 tgcgttgcaa caatcaatac ttttgttcgt tttttgagat gattccttaa ttgatgttgt
    14281 tttgtcgctt gataatagat cgactgtcct gcttttgcca catagaccgt ctctcctaag
    14341 cttaactcta attccccttc taatacaaaa ataaacgttt cagataatga aggttcaaac
    14401 gtcttatatt ctcctgcacc ttcaaaagtc agcaagacag gctccatttc tttttcattc
    14461 gaatcagcca cgagccattc caattcatac ccgttttctt catcgaaata aaccgtatga
    14521 tcttcttttg aataaaccac ttgcagattc gctgcttctt gatgaaaaaa ttcttctgga
    14581 gtcacaccta agacttcaag aatagaaaag aatgtttcca ttgatggtga acttaaatct
    14641 cgttccaact gggaaatata cccttttgac aaatctgtgc gttcgcctag ttcttcttgt
    14701 gtcaaattct tttggatgcg caaatttctt aacttctcac caatttccat aatttcctcc
    14761 gaatcgaagt tttctaatag taaacttcct gtttactacc actcgtatta gtatacgaaa
    14821 tttattgaaa aaggcaaccc tttgtaaaat tttttttgct gaatatttct ttatcttttg
    14881 gaataaattc tagttgtttg attctttatt tttcacctaa tttttgtcta aatttacgga
    14941 agaacaagaa taaaagagta ggctttatgg tatgataaaa gaaaatttta aaaggagtgt
    15001 ctcacgaatg aacgcttttc gatcttttca ggtaataaag gaacctgttt tccatacagc
    15061 aatcagtgat ctgccattta ctgagttggc tgaaaatgaa gtcttggtaa aaattgccta
    15121 ttctgatgtc aattataaag atgcattggc aatgagcgag tctggtcagg tgatacgaac
    15181 atatccgatg actccaggga ttgatttatc cggaactgtc gtccaaagta acaatccacg
    15241 tttttcaaaa gaagatcttg tacttgctac tggttttggg cttggcgtca cccatccagg
    15301 cggttatagc caatatcaaa aagtaccagg agactggtta gttcctctac caaaaaatat
    15361 gacgctgcgt caagcgatga tccttggtac agcagggttt actgctatgt tgtgtgtgaa
    15421 cgctttaatc cgtcaaggta tgacctccga taaaaaagtt gtcgttactg gtgcttctgg
    15481 cggtgtaggc agtacagcga tagccctgtt acataaacta ggattccagt caatcattgc
    15541 cttttccaga aaagaagagt ctgtcacatg gctaaaaagt ctaggtgctt tgcaagtcgt
    15601 acgacctgat gaatttctgc cagagacaac aaaagcttta ggaaaacagc aaatcgacta
    15661 tgtcatcgac acagtaggtg gcgagcagct aagcagtctt cttccattga tctcttataa
    15721 cggtgcagta gctttatgtg gcaatgctgg gggaatcaaa ctaaacgcga ctgtcttgcc
    15781 ttttattctg cgaaacatcc agttgattgg aattgattct gtcaatgtac caatagatca
    15841 acgtctttct ttatggcaac agatggcgga tttgcagatt gcagatgaac tggtcgtcca
    15901 agagattact ctggatcaat tgcctgaaac agcatctaaa ctacttgctg gaacacacca
    15961 aggaagaaca ctagtaaatg taggtgacca taaatgaaga ttctaatcac agctggagga
    16021 acgactgaaa aaatcgatca agtacgtgct attacaaatc attcgacagg acgattaggg
    16081 caagcattag ctgattattt agctgccaat ccggatacca caatcgatta cgtgactacg
    16141 aaacaagcac tgaaaccgaa aagacactcc aatataacga tttatacgat cgaatctgca
    16201 ctggacttat ttttacaatt agaagaacta acgaaaaaag aacactacga tgcgattatc
    16261 cacagtatgg cagtcagtga ttttactcct gcttttagtt tttcagaaga acagctggca
    16321 aaaaacctac ccacttcttc aacgcaagaa gaactggaca attggtttgc ggaaaatgaa
    16381 cagacagata ccactgtttc aaagatctct tctaacaccg agcatcttgt gctcgttttg
    16441 aagaaaacac caaaaatcat ttcttatcta cgtgaatggc agccaaaagc aaaaatcatt
    16501 ggtttcaaac tattagtaga tgtaccaaag gaatctcttc tggcagtcgc aaaaaatagt
    16561 ctgataaata acaaaactga ttttattttc gcaaatgatt tgacggaaat ccatggcgag
    16621 acacaccacg gctatctctt atctaaagat ggaacagtgg aagaagctca aagtaaatct
    16681 gagattgctg cattgattac tgagaaaatt agactggagg aatcaaaatg aaaaagatct
    16741 tactaggcgt tactggaagt atttctgctt acaaaagcgc tgatcttaca agtcaattcg
    16801 tcaaattagg tgcccaggta gatgtgatta tgtctaaaag cagcacacat tttatcacgc
    16861 cattaacctt gcaatctctg tcgaagcgcg cagtccatac agacgtcatg caagaatcaa
    16921 atccgcaggt aatcaatcat atcgaactag caaaacaagc tgacttgttt ctcatcgcgc
    16981 ctgctacagc aaacattatc ggcaagctag caaacggcat tgcagacgat cttctttcaa
    17041 cagtcgcaat ggcgcttcca gtgggaacac caaaattgat tgcccctgcc atgaacacga
    17101 atatgtatca aaacccgatc actcagcgta atctggaaac attgagatct gtcggctatc
    17161 aggaaattga accaagagaa tctcttttag cttgtggaga ttttggaaaa ggtgccttag
    17221 ctgatattca gacgatcatc gaacaggtca tgctgactct taatgaaaga aatgcttaga
    17281 aaaaaggagt acttatgaaa aatacaaaaa actttacatt aactgcgatg tttttagcta
    17341 ttttgatttt attagctgtt acgcctttag gttttatccc tatcggtcca atcaatgcaa
    17401 caaccatgca tattcctgta atcattgctt cgatcgtttt aggaccaaga ctaggtgctt
    17461 ttcttggcgg tacatttgga ctgatcagca tgatccgtag cacagttatc caaacacctt
    17521 tgtcttttgt cttttctccg tttatcccag tcatcggaac agaacatgga agctggaaag
    17581 ccttgctgat cgcgtttatt cctcgcatct tgattggggt ggttccttat ttcgtttata
    17641 aaggtctgcg caaactaatg aaacaaaaag cagattccgt ttctttattc ttagctggac
    17701 tttccggttc attgaccaat acgattttag tgatgaatat gatctacttt ttattccagc
    17761 aagattatgc caaagtcatc ggaacaaatt taaatgctgt ttattcagct attctagcgg
    17821 ttatttttac aagtggtata cctgaaggaa tcgtcgcagg tcttgcgact gcagcagtat
    17881 gcaatgtctt gttaagatac ttcaagcacc cttctcttac taactgatag tcatcgtagc
    17941 cgatctctga tttttttctc ttttggtgtt actattgaat ggaaagattt gacgtcgaaa
    18001 ggatgatcca aatgaaaatt tttgttgtag gtgcaaacgg acaaattggc cgacacctga
    18061 taaaagattt agctccaagt tcccatgaaa tttttgcagg ggtgagagat gttgcttccc
    18121 aaacattagt aaaaaaagag aatgtttcgt atgtctcttt tgatttaact tggtccgtgg
    18181 aaaaaatgac cgaagcattc aaaggcatcg atgtattgat ttttgctgcc ggctctcaag
    18241 gaaagaatct tctgcaagta gacttggatg gcgcaatcaa aacagtgatc gcagctgaaa
    18301 atgcgcatgt ctctcgctat ctgatggtca gtgctgttta tgcagatgaa ccagcaaaat
    18361 ggcctgagtc catgacagat tattatatta ctaaacatta tgcagatgaa tggctgaaac
    18421 gaactaactt agattttgtc attcttcaac cagtcacatt gactaacgat gaagaagtta
    18481 cttccattca attaactaag cctaatgaga aggcatcaaa aacgatcact cgctctacag
    18541 ttgctgctgt acttgccgct ctagtggaag aaacggatat ttctcgtacg actctagtac
    18601 tttctgaagg tagcaaagag ttgaatacag cattccaaga atgggcaaag gaggaataga
    18661 tccatgcgtg ccatttcaat caagcatcct ggttctgtag atgtattaga acaaattgaa
    18721 gtgcctgttc ctaaaccaaa acaaggcgaa ttactgatca aagtccatac agcagctgtc
    18781 aatcgaacag atattatgaa aagagaatca gtctctttga aacctcccta ccctattttg
    18841 ggagtcgaag tagctggaga ggtagttgag aataaaagtg atatctccac ttttactcct
    18901 gggacaaaag tatacggatt agtcaatcta ggtggttatg ccgaatatgc tgtgatgcct
    18961 gccgatcgtg caatccttct ccctgatacg ctagactatg tttctgctgc tggaatctca
    19021 gaagtttttt tgactgctta tcaaacactt tactggcttg gaaaattaca agaaggcgaa
    19081 actgtcttga tccatgcagg agcaagtggt gtgggaacag cagcgatcca attagctaga
    19141 cagctttctc acgcgaaaat catcgttact gcaggatcag aagaaaaact cgccttttgt
    19201 aaagaacttg gtgcagatga attgatcaac tataaaaaac aagactttca agaagaggta
    19261 cgaaaaatca ctgaaggtcg gggagtcgat gttattttgg atttcatcgg cgcttcttac
    19321 tgggaaaaga atttagcttc tatagccgtt gatggacgtt gggtcttgat tggtatgcta
    19381 ggtgggacca tcgtgcctga gatagacctt ggtgtattga ttgggaaacg tgtccaactg
    19441 cttggtacat tattgacacc tagaagtgat cagtataaag cagagctcgc acaaactttt
    19501 atgcaaaaag ctgggccttt tctagaaaat ggcaagattc ggccaatcat tgaccgtaca
    19561 ttccaattca gcgcagtcaa acaagcgcat gaatatatgg aagcaaacaa aaacatcgga
    19621 aaaatcattt tgaagataac tgaataaaat catgctgatc aaaaaagagg ctgggacaga
    19681 agcgtttaac tccaagaaat aagaagaaat tcacgaaaat tgcttttcaa atttttgtga
    19741 atttcagctt atttccgaag gaatttcttc tgcttccgcc gtttatacgt gtttagagcg
    19801 tgagacaaaa gtgttcttta cttttgtccc acgctctttt tccgaataaa cggtgttcaa
    19861 aagcttgcat ctgcggtaat aagtccaacc aagttaaaaa cataagaagc tgtttttact
    19921 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttcac taaacagtgt
    19981 tcaatcagct gaccctcggt attagctcaa aacgctgatt tcacaacctc tttttattta
    20041 cgttctattt cattcatttt ccgaagcaaa tcgagctgct tgtatgcccg cttgtcgccc
    20101 aaaaataatg atctccgcaa ctgaatttcc accgatccgg ttgtttccat gtaagccacc
    20161 tgtgacttca ccagcagcat ataaccccct aattggctgg ttttctttat ctaatacttc
    20221 agtgttcgta ttgattttca caccgcccat cgtgtaatgg attcctggtg cgattttgat
    20281 cgcataataa ttcggagacg ataagtcatg ttccatggct gtggcacggt tgaattgagc
    20341 atcttttttc tccgcaacat ccgtattcca tccatcaact gtttttgcta gtgtttcttt
    20401 tggtaaatcg attttttctg ctaattcttc cattgtcttt ccagttgtga catatccttt
    20461 ttggtcataa aattcaatag ctgtggcacg atcacgcacg ccttggtcaa aaatcaaata
    20521 agctgatttt tcaggcaatg ctgtaattgc tgcagatact ttatcccttg tatccatttc
    20581 attgacaaat cttttcccct cttgatcgac caagattgct ccttctccgc gaacaacctc
    20641 accaatcaag accccctcat cctgctgcac agttggatga atttgtattt tatccatatc
    20701 aacggtttgt ccacctaatt tttcaatcat ggtgatacca tctccagttg atccttcttg
    20761 atttgtcgta atataatcag ctaaatcggg acgatatttt tcaataaatt ctttgctggc
    20821 accaaaaccg cctgtggtaa ctacgattgc ttttccggtg acctcttttg gttcttctcc
    20881 ttgaacatgt acagtcactt cattgaccgt ttccccattt ttcttaatgt ctgtcacatc
    20941 tgcgttgaca aaaataggga tttcccgctc atggacgtta ttcaataacc cctccactaa
    21001 gtatccacca atagccgagc catcagacgg acgatgtgtc ctcttttcac tcatccctcc
    21061 agtgatcgtt aagttgtcca gtttgatacc gttttgatct agccaatcga ccgcatcagc
    21121 agagtgatcg acaaagtatc gaagcatttc tttatcattc gttcctccac caccttttag
    21181 cgtttcttca tagaaggcat cattcgagtc tttgattcct tcttcttttt gaaattttgt
    21241 ttctgaagca ttcataccgc ttgatgattt agatgtgttt cctccggcaa caggcatctt
    21301 ttccaaaatt actggattca acccagcatc ttttgcttcg atagctgctg ccatccctgc
    21361 accaccagct ccaataatca cgacatcata agattctttc atttcttttg gatctgtata
    21421 tacttgttca gaagcgcctg atgatacttc ggcagattcc ttcgtactag aacttgtctg
    21481 cgctgtattt ttctgatctg cgccgcatgc gctcatagca agaagtgcaa cggctgttaa
    21541 taaaccagca actaattttt tcatttgtat cccccttttc tccttatact atattaagtg
    21601 atttttttca caatatcaac tctgtttttc tttaagtcat atagctttgg taaacacacg
    21661 cttcttagta taattaagga tatacatgaa ggagttattt tatgaatcat ttgaaaacct
    21721 tttttacgaa cagtacaaaa actgcaggta gaatcacaaa cggaatctat attttattac
    21781 gtatcatcgt cgttattatg ctagtggatc aggtctggaa ccaacactgg ggaaatacaa
    21841 tgcttttatt actgacatta attttatttg aaatccctta ttttctggaa aagatattaa
    21901 agatacagat tccaaatatg ctagaaatca ttttgttatc ttttattttt agttcaacag
    21961 ttttaggaga gttaagtgat ttttatggct atttttcaat gtgggatact tttctacatg
    22021 cattcagcgg atttttagct gggggtgtcg gcttttccct tgtttatttg ctcaataaaa
    22081 atacaaaaac catgaacttg actcctttat tactagcagt tgtttccttc tgtttttcga
    22141 tgacagttgg tgttatgtgg gagtttgtgg aattttcagc agataaatta ctgcaattcg
    22201 atatgcaaaa agatacgatc atccagcaaa tcaatagtgt ggagttaagt aaagacggaa
    22261 acactgtcta tcatgttcca gatatcactc ggacaatcat tgaaagtcaa aatgcagatg
    22321 gtaaaacaga taaaacaatc atccaaaatg gttacttaga cgtgggaatc atggatacta
    22381 tgaaggattt atttgttaat tttattggag cagttgtttt cagtgtattt ggttacctct
    22441 atagtcgaaa cgattaccaa aaatttacgt tcatcaagaa ttttattcct aaaaaaatca
    22501 aacg
//
LOCUS       DAYYGS010000048        22165 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000048 DAYYGS010000000
VERSION     DAYYGS010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22165
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.048"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            230..2065
                     /gene="tdc"
                     /locus_tag="ACHA44_001691"
     CDS             230..2065
                     /gene="tdc"
                     /locus_tag="ACHA44_001691"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HFM8904964.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            2370..3848
                     /locus_tag="ACHA44_001692"
     CDS             2370..3848
                     /locus_tag="ACHA44_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8904965.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            3814..5145
                     /locus_tag="ACHA44_001693"
     CDS             3814..5145
                     /locus_tag="ACHA44_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8904966.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            5259..7811
                     /locus_tag="ACHA44_001694"
     CDS             5259..7811
                     /locus_tag="ACHA44_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HFM8904967.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(7818..9257)
                     /locus_tag="ACHA44_001695"
     CDS             complement(7818..9257)
                     /locus_tag="ACHA44_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8904968.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            9232..9414
                     /locus_tag="ACHA44_001696"
     CDS             9232..9414
                     /locus_tag="ACHA44_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8904969.1"
                     /translation="MIFFISEYINQTPFYSKNTEKINGGQVSTDFSIIVYFSGAFTDD
                     KNFIMNLKNDTTKIKK"
     gene            9603..9965
                     /locus_tag="ACHA44_001697"
     CDS             9603..9965
                     /locus_tag="ACHA44_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFM8904970.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            complement(10152..11009)
                     /locus_tag="ACHA44_001698"
     CDS             complement(10152..11009)
                     /locus_tag="ACHA44_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8904971.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            11129..12040
                     /locus_tag="ACHA44_001699"
     CDS             11129..12040
                     /locus_tag="ACHA44_001699"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFM8904972.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHA44_001700"
                     /pseudo
     CDS             12047..13698
                     /gene="mdcA"
                     /locus_tag="ACHA44_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015451 - decarboxylation-driven active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            13800..14099
                     /locus_tag="ACHA44_001701"
     CDS             13800..14099
                     /locus_tag="ACHA44_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HFM8904973.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHA44_001702"
     CDS             14089..15765
                     /gene="mdcD"
                     /locus_tag="ACHA44_001702"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HFM8904974.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            15769..16362
                     /locus_tag="ACHA44_001703"
     CDS             15769..16362
                     /locus_tag="ACHA44_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HFM8904975.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            16386..17462
                     /locus_tag="ACHA44_001704"
     CDS             16386..17462
                     /locus_tag="ACHA44_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFM8904976.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHA44_001705"
     CDS             17487..18356
                     /gene="mdcB"
                     /locus_tag="ACHA44_001705"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019361 -
                     2'-(5''-triphosphoribosyl)-3'-dephospho-CoA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HFM8904977.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            18467..19231
                     /locus_tag="ACHA44_001706"
     CDS             18467..19231
                     /locus_tag="ACHA44_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HFM8904978.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            19720..21813
                     /locus_tag="ACHA44_001707"
     CDS             19720..21813
                     /locus_tag="ACHA44_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8904979.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            21838..22140
                     /locus_tag="ACHA44_001708"
     CDS             21838..22140
                     /locus_tag="ACHA44_001708"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8904980.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         7179 a         3787 c         4722 g         6477 t
ORIGIN      
        1 ctttttttag gttatctatt ttagaagact gatttattga aaaattgttc gcttttttgc
       61 gttgaattat cagaaaaatc cgactttatt tatttttgtt tgtaaaaatc tacgtggatt
      121 tgatgttata aaaaaaaaat tctcattttt ggcgctatag tgaagaggtg ttacagataa
      181 acacatgtat ctgcaagaca ttgaccaaca aaatatggag ggaagaaaaa tgaaagatat
      241 ggatatcaag gccgtcttta taggcgacaa agctgaaaat ggaccagttt ataaaatgtt
      301 gttgaacaag atggtagatg aacatcttgg atggagagaa aattatattc cttcagatat
      361 gcctgcaatt agcgaaggag ataagctcac tccagattat ctagccaccc gcgaccatat
      421 gatagaggtc ttggatgaag tcagtcagcg attacgcgca ggatcgatcc catggcattc
      481 agcaggccgt tactggggac aaatgaatgc tgaaacattg atgcctgctt tattagctta
      541 caactatgcg atgctgtgga acccaaataa tgttgcattg gaatcttcta tggcaacttc
      601 gcaaatggaa gcagaagtag gacaggattt tgcttcatta ttcaatatga cagacggatg
      661 gggacacatt gcagcagatg gttctattgc taatcttgaa ggattatggt acgcccgttg
      721 catcaaatcg attcctttag ctgtcaagga agtgctgcct gaaaaagtga aaaaaatgtc
      781 tgagtgggaa cttttgaatt tatctgttga agaaattttg gaaatgaccg aaagctttac
      841 ggacgaagaa atggatgagg tcaaagcagc ttcttcccgt agcggaaaaa atatccaaag
      901 attagggaaa tggctggtcc ctcaaacaaa acactattct tggatgaagg cgttagatat
      961 ttgcggagtc ggcttagatc agatggttgc tatcccagtt caagaggatt atcgaatgga
     1021 tattaatgct ttggaaaaaa caattcgtga attagctgga caaaaaatcc caattctagg
     1081 tgtggttgct gtcgtaggaa cgacagaaga aggtcaagta gacagtgtag ataaaatcgt
     1141 ccaattaaga gaaaggttaa aagatgaagg gatctatttc tatttacatg ttgatgccgc
     1201 atatggcggc tatgcgcgtt cactgtttct aaacgaagca ggagaatttg tgccgtatgc
     1261 ttccttagct gaattctttg aagaacatca tgttttccac cactgcgtaa cgattgacaa
     1321 agaggtatat gaagggttca gagctatttc agaagcggat tctgtcacga tagatcctca
     1381 taagatgggc tatgtccctt atgctgctgg aggaatcgta atcaaacata aaaatatgag
     1441 aaatatcatt tcttatttcg cgccatatgt gtttgaaaaa tcagtaaagg ctccagatat
     1501 gttaggagct tatattttgg aaggatcaaa agctggagca accgccgccg cagtctggac
     1561 agcacatcgt gtattgccat tgaatgtgac cggatatggt caattgattg gggcttcaat
     1621 cgaggcagct caaagattca gagaatttct cgatcatctg acttttactg ttaaaggaaa
     1681 aacaatcgaa gtctatccat tgaatcatcc tgattttaat atggtcaatt gggtattcaa
     1741 agaacaaggc tgtacagatt tgaacgctat caatgaatta aatgaaaaaa tgttcgatcg
     1801 gtcttcttat atggatggcg atgtttatgg tgaacggttt attacttctc atactacatt
     1861 tacacaagaa gattacggtg attctccgat tcgttttgtt gaaagaatgg gtctaacgaa
     1921 agaagaatgg aaaaaagaac agaaaatcac tctgctacgt gcggctatca tgactcctta
     1981 tttgaatgat gatcgaattt tcaattttta tacaaaaaaa atcgccaaag caatggaaaa
     2041 gaaactaaac gaaatcatcc aatagaactg atttttgtgt gaaagaataa caagagacat
     2101 atggagaaca gacaattatt tgcttcatca aaacaattgt atttccccct gtttttcata
     2161 tattcctcgc caagatattc ttttttataa aacgttctat aagttggatc gttcgtttca
     2221 tgtatcaaac ccagttcact aagatcgttc aatggatgtt tcggtgatac tgggttttgt
     2281 actgaatcga tgaaaccttg tcatttctgt tcttgaacaa aaataaaaga aaacaaaaac
     2341 atatgtcgct attgaaagaa ggaataaaga tgaaagatac tggttcatca aagcaaatca
     2401 cgaccatgac ctttatcgga atgacctgcg cccttgtagc aagtgtacga aatattccag
     2461 atgtagcagc aacgagctgg acgatgattt tttacatgct ggttgcagta ttattgtacg
     2521 cttttccaat ctcattgatc tctggtgaat ttgctggaat gtttccccaa aaaggtggtc
     2581 cagaactttg ggtatcgaat gctctaggaa aaaaatgggg gttcgttgtc tcatggctgt
     2641 tatgggtcca aatgtttcct gggatggtga tggtcgcttc agcactcgct ccgttgtttg
     2701 gtaatattat cgataatgtg ccgcttggtt tgaacagcaa atttacatta gttgtcattt
     2761 tagtcgttta ttggatcatc acttttttga atttgaaatt tgatatggca aaaatcggcg
     2821 gaaaagtcgg tgtttggtta ggattgtacg tgcctttgac gatgatgctc ttattagggt
     2881 ttgctgcgtg gatcaaagtt gggatcgtac caaccggaac attaggcagt ttttcatggg
     2941 acaaattaat accagatact acgactgcat cctcatttgt ttattttgcg ccgatcatgt
     3001 ttatttttac tggtatcgaa atgtcctctg tgtatatcac tcgattggaa aatccagtta
     3061 aaacgtatat tcgcggagta tttgccgcgt taatttttat atttttcgtc aatattttga
     3121 atgctttagt tgtagcaaat gtcgtaccaa aaggacaaat ggaattgaat aatatcgcgc
     3181 aatctatttc gatttattgc caaattcttg gtttgccaca tcttattgtg aatctgttta
     3241 gtttacttgt cttcattggt gtagcagtcc aattatctgc ttgggcctca ggaccagcaa
     3301 agacagtgac agaaagtgct agaaaaggtg cgtatccacc aaaattcaat ttttggaaga
     3361 cgaatcaatt tgacgtatcc aaatccgtta ttttgactca gtctgtcatt atctcaatct
     3421 ttgcactttt ttatttgctg attcctggcg tcaatcaagc atttttgatg ctcgtaaatt
     3481 caactactgt tatctactgt atcgtctatg tcatcatggg gatcgcagtt ttgagattgc
     3541 gttatacaca tgcaaagttg aatcgaccat ttagaattgg aaaaaaagag aaaaagagca
     3601 atcttggagt ttggactgtc gttattgttt tatttgcggc aatcggattt tctgtcggac
     3661 tgacgatgaa agctggaaca tggatcaatc taattgcagt cacagccatc agtgtcattt
     3721 tgtttgtagt accactctgt attgaaaaaa taaagaaacc ttcttgggaa caggaagtcg
     3781 ccatgctgct tgaaaaagaa aaaggtgaac ttgatggaaa aaccggcaat gaaacggact
     3841 ctaactaatc gaaatatcca gttaatcgca ttaggtggcg caattggaac aggattattt
     3901 ttaggttcag caaaggcaat tgcatcagca gggccagctg ttattctcgt ttatttgata
     3961 tgcggtttgg ctatttatgg tttgatgcgg gccatgggtg atttattttt agcaaatagt
     4021 acatttaact ctatcagtga tttcgttgac tattatttag gcaaaaagtg ggcatttttt
     4081 gtcaattgga cttattggct ttgctggctt ggagctggac ttgcggaatt aacagcaata
     4141 ggattgtata tccatttctg gtttgtacat gtaccagctt tgataagcgg aggtagcgca
     4201 ctaatgatct taatggcagt caaccttgtt gcagtcaagt ggtttggtga attggaatct
     4261 tttttctcaa tcatcaagat cttggggatt ttccttttta tcttattggg tataggattg
     4321 agtgtctcag attttcatac ccagtctatc ggaatcaaag aaaattttgg acagatcgat
     4381 gaattactac cgtttggctt tgctggaatg gtagtctcat ttcctatggt tttattcagt
     4441 tttgctggaa ttgaaatgat cggcttaacc gttggcgaga caagtgatcc gcaaaagaat
     4501 ttgaaaaaag caatcaactg tctgccttgg cgtattctct ttttctatat aggaacgatt
     4561 ttgtcgattt tatttgttgt tccatggcat tgtttagatt taaaagaaag tcctttagtg
     4621 actatgctgc aagtgatcca ttgccaaggc ggagcagcaa ttgtcaatct aatgattttg
     4681 gttgcttctt tgtcctcagc aaatagtgca gtctacagta ctagcaggat tttatatcaa
     4741 acagcaaaag aaaaaagttt gccccattgg ttttgtgcgc tttctaagaa aggcgtacct
     4801 ttacacggta ttttaattac aggaatgtta ttttttgccg gtctttgtct acattttttt
     4861 gtagctgata gtcgattgtt ttttcagctt ttggcaggca tgacaacgag ttgttttcta
     4921 tttgtgtggt cttcgatctt gtgtgcacat gttcaattta ttaaagaaaa gaaacggaac
     4981 actacacaaa gatataaaga tcttgctttg ttggtgtttt ttacttcaat tgctttcagc
     5041 ttgttattcg aaagcacgac acgctttatt ccttttttat tggttatctg gtttgtattc
     5101 ttaaattttg tatataaaag aataagcaat agaaatatca gttaggccca gatacatttt
     5161 gcaaaacgaa ttatttcaga cccccctgaa ataatcgata cgcctgaaaa aacttccctg
     5221 cgtataataa cagtatcaat taaacaggag gggataggat gaactggtac agattgacgg
     5281 tagaagatgt attgaaaaac tatcaaacat caacagaagg cttgtcgcaa gaagaacgac
     5341 agaaacgcct gactgatcaa ggatataatg aaataaaagc aaggcagcag acaaaacgat
     5401 ggaaaaaaat agcaaagcat tttactgatc tgctgatgat cgtgttgatc attgcctctt
     5461 ttttgaaatt tgcttctagt gaatatattg aaggaagtat tattttattc gtagtgatcg
     5521 tcaatggact cgttagttac tggcaagaaa gaaaagcgga agaatcattg aatggtttga
     5581 agcagctgat gggacaagaa gcgatagtac tatcaaacgg gatgcaagaa aaaatccctg
     5641 cgaaagaact ggtaaaagga gatgtcattg tcttacgtgc cggagatgtc gttccagcag
     5701 atatccgatt aatcgaagca catgatttat tagtggaaga atcgattttg acgggagaat
     5761 cagaagcatc tgaaaaaagt catgccgttt tgacagaaga agaatcgatc ggcgaccaga
     5821 aaaatatggg tttttcagga acgcttgtac aatctggttc tgcagtcggt gtcgttgtag
     5881 aaacaggaaa cacgacagaa atcggtaaaa tcaatcaagc attgcaatcg gtcgaacaac
     5941 aaacgacgcc tctgattaaa aaaatcaacc gattaaacaa acaaattttt caaggcattc
     6001 tttgtttgag cctattctta gttattttta cgacatttcg atatggtatg gattggcata
     6061 tccttctttc ggctacgatt gctttggtgg tgtcaatggt acccgaagga ttgccagctg
     6121 ttttgaccat gattctatcc gttggagtac atgaaatggc aaaagaaaag gcgatcatca
     6181 aaggacttcc ttcggtagaa acattaggtt ctatgacagt gatctgttca gataagacag
     6241 gaacattgac aaaaaatgaa atgacagttg tggatgtagc tgcaaaagaa gaagcctgcg
     6301 tgttgtccat tatgaaaaac tgtcaggaat taaaaacaaa agaagaacag aaaacagaaa
     6361 atttaagtgg caatccgaca gaagttgcgc tgattcgaca cacagaaaca gccaatctac
     6421 ctttgaaaaa aatagaagca aagattccgt tcagttctga atacaaatat atggcaacga
     6481 tgcatgcgga ggaaaaagga gcggtgattt acgtcaaagg tgcaccagaa gtattatttg
     6541 ctaaatcaac tctttcttct gcagaacaag aagcatggag tcagacagct gcagaatttg
     6601 cgagaaaagg tcagcgtgtt ttaggatttg cttataaaaa agtagattcg aaacaggaac
     6661 tcacgcatga gactttaacc caattaactt ttgcagggat cgccggattg atcgatccgc
     6721 caaaagaaag tgcagtcaaa gctgtaaagg aatgccaaca agcagggatc tcagtgaaaa
     6781 tgattacagg ggatcacaaa gacacagcaa aagcgattgc ggaacaaatt ggcttaaaac
     6841 atactgccaa agttttagaa gggatcgatt tggatcttat gtctgatgaa gagttgattc
     6901 agcaagtgcc aatagtcgat gtctttgctc gaacgacacc agaacataaa ctgcgaattg
     6961 ttaaagcttt gcagaaaaat ggagaaatcg tcggaatgac cggagacggc gtaaatgatg
     7021 cacctgcttt gaaaagatcc gatgtaggaa ttgctatggg aatcaaaggt agtgaagtaa
     7081 gcaaacaggc agctgatatg gttttaggag atgataattt ccatacgatt gcaaaagccg
     7141 taaaagaggg ccggcggatt ttggataatt tgcaaaaaac catcaatttc ttcttgccaa
     7201 ctgcattagc tcaaggattg atcttgattt gggcacttat gctgaatcgc ccgctacctt
     7261 tgtccccagt acagatttta tgggtcaata tggtaacgac aatcacttta tcttatgcac
     7321 tagggttcga gtcgccagat ccagaaatca tgaagcgccc accaagagat ccaaagcaag
     7381 gaatcctatc tggttatcat ttgtttcgga tcatctatgt ctccctactg atcatggttc
     7441 ctgcctattt attggctatg cagtttgaag ggcaaagctt acagcaaacc attcttctgc
     7501 aaaatatcgt cttagctcaa gctgtttaca tgatcaattg cagagagttg tccaagccgt
     7561 cattgaacca aggtcttttg caaaacaaag cgctttttct ttcattaggt atccttgctt
     7621 tattgcaagg gatggttatt tttctaccag tcggccagca gctgatcggt acagtaagtt
     7681 taacgatgac acaacagtta ttgattggag caaatgtgat tcttctattc ttagttgtag
     7741 aaatagaaaa agggatcatg aacaaactgt ttcgcaaaaa agaaaaaaca gctagagaat
     7801 acagtcgcta gccgttacta taaaaaagtt ttgaaagtga gttcagtcaa acgattgagc
     7861 tcatttttcg ttggaataga agaaatgtat acaataggga tatctagaga atctagaggg
     7921 aaattggaaa tcaagatatc tgttttttca aaagaaatag tggaaagatc ggttagttcg
     7981 ataggtaaga agcagacacg ttttcctaag taatctttta attcctgctg caagaacgcc
     8041 ttccatgaag gttctccttg gaaaataaaa tagacattga acaaattcgg tttgatagaa
     8101 aaaatcgttt gttggatcag tagagacaag agttggagaa agtaatttgg ttcattgatt
     8161 tctatttctt tttttagatc gttcagtaaa ttctctttct gttgatacaa gtgtgcatat
     8221 tgctgatcat atgtttctaa aaaacgatgg tattgacggt ccaatgcttc aatcaatatc
     8281 ggcgattcat gatattttaa tatgaggaga gccaagttat cagtcagaga tgtttttttt
     8341 agttgatcaa gagacagctc tgaagctagt tcctctacaa acgtttcgag gacctctaaa
     8401 aatggagaat gatgcaggca gcgagcggtt tcttgtccat agttattgtt gtaattccaa
     8461 ctttccagaa agcagaaaga agcaaaagaa agctcagtgt ctttaagata aataccttcc
     8521 gttaaataca attttttcaa tgcagagtca tacagggagg agagtgtgct gttggtatgg
     8581 atagaagaat ctaatcggca attcgctttt atacggatcg tattgataaa gaaccaacat
     8641 gttccgaaaa tctcttctgt ttcaaccgct agttcagttt gactgaggtt tcttaaaaat
     8701 tgaaaataat gggattcatg gatcgaataa tcctcgaaaa aatagttgtt atgagtgaaa
     8761 ggaatcagca atcgatgata aaaagtacga atcgcacgct cttttccaag aatacaagct
     8821 gtatatttcg tcaaatcgat ctgcaagtcg taacttgcta aacgctgatt gattttagtc
     8881 acatgtcttt tcaatgtccc ataagaaagc ccatggtcgg ctagaaaatc agggacagca
     8941 actttttttg taaaaagcag atttttcagc agttcgacac tgactgattg tttgaaaaaa
     9001 atctcccaaa ggtcatttgt taataaattt tgctggtttt gcaaactgat tcctgcttga
     9061 tcgctgatga tgtcccagcc ttcgggcagc tgagagcgaa tagattgaag gtcagaaaaa
     9121 atcgttcgtt cggttgtccc tatgatttca gctaattcat ttacggttat ctttgtatga
     9181 ttcagcaatt gttctactaa agaaacatga cgtcgaatat ctttttcggt aatgattttt
     9241 ttcattagtg aatacatcaa tcagactccc ttctattcta agaatacgga gaaaataaac
     9301 ggtggtcaag tttcaactga tttttcaatc attgtttatt tttctggagc atttactgat
     9361 gataagaatt tcatcatgaa cttgaaaaat gataccacta aaatcaaaaa ataggtgggc
     9421 aaacaataat aaacatttta atagtagatg gtagcaagac caacacaggc ggattgaaca
     9481 tttaagagtt gataaaaaag aaaaatatcg aagtatttga ttagcaacta cttgtattca
     9541 tttttctatt cttacgtggt atctttgaat aaagcgaatc aataagaaag agatggaagc
     9601 agatgctaac gaaaaaagaa ctaatcgatc acatcaatga aacatacaat ttgtctccag
     9661 aatatatttt taaaaaattt cctgattatt gcgtattcag acatcaaaaa agcaaaaaat
     9721 ggtttggggt cctgatgtct gttccagaag aaaaaatcta tgatgatgca agtgaccata
     9781 ggatcatcaa tattcttgat ttgaaggtcg atcctgaact tggcggaatt ttgcgaggaa
     9841 aagaggggta ttttccagcc tatcacatga ataaagaaca ttggatctct attgatctac
     9901 gtaaaataga aaagctagat gaactagcag atttgattga tggaagtttt aaatcaacgc
     9961 aatgaatgtc taggacaata gagaagaggt tgtgacagaa atgtttagct tcaagaaata
    10021 agaaggaatt tacgaaaatt gcttctcaaa tttttgggaa atttcggctt attttctaag
    10081 aaattacttc tggaacaccg tttgttcgaa aaaagagctg cgacaagaat tttgtcccag
    10141 ctcttttttt tttattttcc taaataaaat tcccgcatcg tttttgcaat cattttgatc
    10201 aaaggatgat cttctgtgaa gctagaagat aaataaattg ttcgtgtcaa gggcaaatct
    10261 ttgatttccc gcaagacgaa aggatcattt ttatcgtaag accaagaaac ttttgggaaa
    10321 aagctaacag ctaatccttg cttgacaata gaacgtaatg tcgcaggatc atctgtcgtc
    10381 gaactgatcg tcaattcgat acttttcgct tcaagtgctg catccagtgt atgacggaag
    10441 gcgttgctct ttgacagcat caagatgggg gtgcgttgga tatcttctaa ttgcagatgt
    10501 tcttttttag caagaggatg tttcttagga atggcgacat aaacaggttc tttcatgata
    10561 ggaatattgg tcatgccagg cttcatctcc gaagtgatgt acagtacagg atcggtaata
    10621 tcatctggta aatgatgttg catgatcgtt aattggatat caggatgctt ttccataaaa
    10681 attcggacca agttcggaat agaaacagag gctacctcta aatacaatat gacggaagta
    10741 acgatcttct cattgaccga agacaataat ttgattcctt cttctaatgt gtctaatgtc
    10801 ttgactgcat atggataaaa ttcttctgca taaacggtcg tctcaatgtt tctgccgcta
    10861 tggtaaaaca aagggacacc taattcgttt tccaaatgtt tgatcgcacg gctcaaggca
    10921 ggttgagaaa taaataattc tttggctgct tgggtaacat tttttgtttc gaatactttt
    10981 ttaaaatact gcagatgaag caatttcata attatccatc ccatccatac ctttttgtta
    11041 tggttttttc tttttttggc attttttatt atagcagaaa ttgactaaga tagcagtgga
    11101 aaaagtaagt gctgaggagg cgaggctcat gagtacaggt ttcttatttc ctggacaagg
    11161 cagtcagaag ttagggatgt tagcggatat ccctgaaatg tacatggaac gtttagaaaa
    11221 tgtgacgggg tatcggttag ttgataaaga agaaaactat caagatacgg tttttatcca
    11281 gctagcatta ctgacaaaag cagtatttta tttagatgaa atggaaaaaa acggtattca
    11341 gcctgatatt gcagcaggac attcgattgg tgctttttgt gcagctgttg cttgtcgatc
    11401 tttgagattt gagaatgcag cggaactagt ctatcaccgc gcagtcttaa tgaaagaagc
    11461 ttatccaagc ggttatgcaa tgggcgtagt agtaggtgtg acgagaagcg aagctgagga
    11521 aattgtagca aaaacatttg atcctgacca tccagtttat ctgtcaaatg aaaattgtcc
    11581 gatgcaacat acgttatctg ggagtatcga aggtttatcc gttacgttgg cagaagccaa
    11641 acggcaccaa gcgcaaacag cgaagctgct gaaagtccct gtgccatctc actgtctctt
    11701 gatgaatgat acggtcaaga aattcaagcc gtatatgcag tctgtccatc tatctgatgc
    11761 tagatgtttg tatataaaaa atacggatgg aagagccact caggacgcag aagagattcg
    11821 taaagatttg ttggaaaacc tagctttccc tgtgcagtgg aaccaaatga tggacttggc
    11881 aaaagaatta ggaatggatc tgacgattga atttcccccc ggcaatactt tgacaaagct
    11941 gattcataca aaatttggtg tagataaagg gatccgtacg atcaatctag accaacatgg
    12001 tattgatgac gccacgtttc tatatcaaaa atggagatga ctaattatgg aaaaaccaaa
    12061 ccgcaactgg gcttctaaac gcgaaaaaaa agcgcagcga ttggaaaaaa tcagcaactt
    12121 gctcaatggc aagtatgtag aaactgaaaa aatcgtagaa gtaatggaaa aactgattgc
    12181 tcccggcgac aaagttgtat tagaaggcga caatcaaaaa caagcaagtt ttctttcaga
    12241 aagtcttgaa aaagtagatg cagaaaaagt gaatgatctg catttgatca tgtctagtat
    12301 ctcacgacca gaacatttga atatttttga aaaaggaatc gcaagcaaga tcgatttctc
    12361 ttatgctggt gcacaaagct tgcgtgtcgc ccaaatgatt gaagacggca caatgaaatt
    12421 aggtgatatc catacttatt tggaacttta tggacgctta ttcatcgatt tgattccaaa
    12481 tgttgtttta gtagctgcag ataaagcaga taaagacggc aacttgtaca caggtttcaa
    12541 tacggaagaa acaccaacaa tcatcgaagc tgcagctttc aaagatggta tcgtgatcgt
    12601 tcaagttaac gaagttgtcg attctttacc acgtgtagat atccctggtg actgggtaga
    12661 cgttgtcgtg aaagcagata aaccttatca attagaagct ttattcacac gagatcctca
    12721 aaacatcaca gaattgcaaa ttctaatggc aatgatggca atcaaaggaa tctacgcaga
    12781 acacggcgta caatctttga atcacggtat tggattcaat acagcagcta tcgaattgtt
    12841 actgccaact tatggtgaat cactagggtt aaaagggaaa atcgcgaaga actgggtatt
    12901 gaacccacat ccaacgatga ttccagccat cgaatcaggt tgggttgaaa gcatccatag
    12961 ttttggtggc gaagttggta tggaaaaata tattgctgct cgcccagatg tcttttttgt
    13021 cggtaaagac ggcacgatgc gcagtaaccg aacattatca caagctgcag gacaatatgc
    13081 catcgatatg tttatcggtt caaccttgca attagattac gaaggaaact cttctaccgt
    13141 aacaaaagga cgtttatctg gttttggcgg tgcgcctaac atgggacata atcctggcgg
    13201 tcgtcgtcat tctacaccag catggcaatc acttcgcgac aatgatgatc cattagggaa
    13261 aggccaaaaa ttagttgttc aaatggttga aacatatggt tcaaataaaa aaccagtctt
    13321 tgtcgaagaa ttagatgcac tagctgttca aaaacaagca ggcttagcca atgcaatgca
    13381 ccagtgatga tttatagcga agatactaca catatcgtga cagaagaagg aattgcttat
    13441 ttgtacaaag caaaaacaaa agaagaacga caagcagcaa ttgcagcaat tggcggtgtt
    13501 acacctcttg gaatgactag cacgaaagaa tcactagata gcttgcgtgc agccggcatc
    13561 gtgaaattac cagaagatct aggcatcaaa cgtagtgaag cgaaacgttc attattagct
    13621 gcacaaacca tcgatgattt gatcgaatgg tcagatggcc tgtatgaacc accaatgaaa
    13681 ttcagaactt ggtcataaaa ttggcgagaa aaaccttttg tcgccattta cccagatgga
    13741 agaagaaaga cgaataagca gaacgtgtat gctctgtttt acaaaaaggg gagagatcca
    13801 tggaacaatt agcttataaa tatccaacga aacagccgat caataagaaa atccatgtag
    13861 gagtggtcgg ttcaggagat ttggaaatct tgatgtttcc aacagaaaaa acagaatcta
    13921 ctgtaaaaat ttgtacagga agcgatggct tcggcgaagt atggaacaat gtattaacga
    13981 gattttttga tcgttatcca atttcagcag atatcgtgat caatgacttc ggtgctacac
    14041 caggagttgt gtacctacga ttgacgcaag caatggagga actttcagat gaaaaatagt
    14101 tttgtagaat taaacgccag agaacgagtg aacgctctac ttgacaatgg acgtgaattg
    14161 attgatccat tcgatcaaat catcgcgcca agtttaccag cacaaggaat cgtccctgaa
    14221 agtgatgacg ggatcgttgt cagccgtggc acgatcaatg gcaaacaagc agtagtaatc
    14281 gctatggaag gaaaattcca aggtggcggt atcggtgaag tttccggtgc aaaaatcgtt
    14341 gctgcacttg aacatgtgtt agaagaaaat aaaaaaggaa atgagatctt tccagtcatt
    14401 gtactagata caggtggtgt tcgtttgcaa gaagcaaact acggactgtt atctatttca
    14461 gaaatcggaa atgtaatcat tgctctaaaa caatatgtac cagtcatcgg tttgattcca
    14521 ggacgtgtag gatcatttgg cggtatgtcg attacttcag cattaatgag ttatttaatc
    14581 gcaacaaaaa aagcgagagt cggtttgaat ggaccagaag ttattgaaca agaagcaggc
    14641 gttcgagaat ttgattctag tgacaaagac ttgatttgga atacattagg atctcgtcaa
    14701 cgggtagaat caaaaatcat tgatgaatta gtgactgata gtgtagaagc aatcaaagct
    14761 gctgtcatcg cagcaatgga cgaacgaaaa gacacgcatc gcagtgaaag ctcagcattt
    14821 tatttatcat tacttgattc tttagatctt tcaaaaccaa tggccattga cgattacaat
    14881 gaagcaattg ctaaccatca agcaaaatca attgacttac cagaagtagc agaaggaaat
    14941 gaaccagctg cagcaagtgt tggttacgat tggtttgcgg cactaactgg catgaaaaat
    15001 ccaacttctt ctattagtac tgtgtattat ggtaaatcaa gtaaattcat agaagaagcc
    15061 atcgtgacat caatcgtacc tgatcctaaa aacccgatgt accgtgtacg aaacggtgaa
    15121 gttggtttag ttgaaggttt ccgcatggga caaattttgg atcacgttta cgaagaagat
    15181 aaagacaaaa aagtgaaacg gccaatcatc gttgtgatcg atgtaccaag ccaagcatac
    15241 ggctataacg aagaattgat cggtatccat gtggctttgg ctaatagtgc tgctgcttat
    15301 gcaaaactcc gtcaagctgg acatccagtt attggattga ttgcaggaaa tgctatttca
    15361 ggtgcatttt tagcacatgg attacaatca agccgcttga ttgctttaaa caataaagaa
    15421 attaccgtcc aagcgatgtc taaagaaagt gctgcacgtg tgacaaaacg ttcggttgct
    15481 gaaatcgaag cagctgctga aaaagttcca gctatcgctt acgatatcaa taattataca
    15541 aaactaggag cgatctatca tttcctagaa gatatcaccg accaagctgc ttcagaacaa
    15601 aatgtcagca aagtcatcga agcaatcaat gaagccattc aagatgtccg caatacaaat
    15661 gacacgatgc tgacaaatcg atacacaaac gaaacagcca aaacattcgg acgtgtcgaa
    15721 acaaataaag tccgtgccaa aatggacgaa gaatgggatg cataaaaaat gattcaagca
    15781 catgatattg tcctttttga taaacatgaa atagaagggt tcaccttgcc tgaatgggtc
    15841 gttctaactg aaaagatgtt cggaacagtt cgtcgtgcgt cgacggatca cgcagaaata
    15901 ctaccgattg gtttacgtgg acctgcccgc aatcagagat ggtcaacaga aatctcaaaa
    15961 catgccgtca aacaagtcat tcatccgtgg gacatcatcg aacaagaatc gtatagacaa
    16021 gagaaaatca tggattatcc tgtttatcag caatttcatg ctgctagaaa catattatca
    16081 ggatgtaaat ggggaatcgg cggcagctta ggatttgaat tatcgactgg gattccggca
    16141 gtaaaagaaa ccagtgattt tgatttgctg ctgtacgcaa attcaccgat tgaattgcca
    16201 attcaggtaa ttcaatcgca tcctgctttt tttgagcaat tcgataccca agtagtgact
    16261 cggcaaggcg gtttttcact aaaagagtat ttgagagagc cagagaaaaa aattctattg
    16321 aaaacagtcg aaggacctaa attaaccaaa gaactttggt aataaagaaa aagagagaga
    16381 aagagatgtt tgaaaacaaa aaaatcaaaa tcaacgtaag tgacttccag aaagtatcag
    16441 ctgtaacggc tgttatgatg caaacgatat tgagctttgc tttggcaaac agccacagcg
    16501 caacaaatac agcttttctt ggtacctttt acgtcatcgt taaatacaca gcaccaatgt
    16561 ttatttttgc aattgtttat aatatggtaa aaacaagtca gcatttgtct tattgggaat
    16621 ttttgaaaga aaagtttttt gaactagtcg ttccgtatct tctatggacg acggcttatc
    16681 tttggctatt tccagcagtc caacaacaaa ctccgtatac ggatactgca agctttttac
    16741 tgaaatatgt cacaggtgat ggagcgcctc atttatggta taccgttatg atgctgcaga
    16801 tccaattgtt catgccgttt tttgtttggc tcggttacaa agtatttgct aataaaaaat
    16861 atgtatggcc agtattgatt ggttcaacga ttttatatgt cgcatggtat ctgttctatc
    16921 aaacacaagt attgactggg ccttatcatg acagctggta cttattagat cggtttgttg
    16981 ctagtttcat gatttatggg gtagccgcat ttgtttatca cgaaaaagtc tatcaatatt
    17041 tggacagagt acgttatctt ttcttacctg ttgccttagt tatcgcgttc ttttctgtcc
    17101 gttcattgct cgcacatcca ggagacttga gctttgcgaa tgcaccttac ttgaatacga
    17161 tccaaagttt atacagttta gtcatcatct ttgctgtctt cataggcgct tccaaaatga
    17221 ttgtaaacga ttcgccaaaa ttgccattgt tcaaatggtt gtctgtttat gcttatcgta
    17281 cgtatttagc caatgtattt gttttccaag tactgttatt attgtttaaa gatagttggc
    17341 tgcagttacc ttcaggagtc atgattcttg tagcttatct aatgacggca agttgtgcat
    17401 ttgctttaag ctggttactt catatcattt gggtagctat caaaaaaggt ttcagcaagt
    17461 aatagtagga agaaggagtg agtgtcatga taaatgacga acgaaaaaaa gcagtgactc
    17521 agcaactcgc tgcgttggta gaggaagcat tgatcgcaga agtaacatta agtccaaaac
    17581 ctggattagt ggatgcactg gatgcgggtg cacattccga tatggactat gctttatttc
    17641 ttaagtctgc taagacgcta actccttttt ttgaagaaat ggctcaaata gcatggcatc
    17701 atgctattga tcaggagtta agagaaagaa ttgctgagat tgggcgaaaa gcggaaaagg
    17761 caatgctgat tgctacaaat ggcatcaata cccataaagg agcgatttgg gcaatggggc
    17821 tattgatcag tgtcattgca caaaaaatca gtatggaaca gcctttttct ttatcgaaaa
    17881 tacttgatga tgtcagcaag cttgctgctt ttcctgatac aaaatatcgt cataaaaaaa
    17941 ccactcatgg tcatagggtg aagcaaaaat atggcgtaaa tggtgcatat gaagaagcga
    18001 tattagggta tccgcatatc aggcttgcaa ttcaagcagc taaggagcta gataatgatt
    18061 catatgtaca gaaacaaatg catatgttgt tagttttgat gagttcgcta gatgacacat
    18121 gcgtattgca tcgcagtgat cagaaaacgt tagtggaaat gcaaaaactt gctcatgctg
    18181 ctagtcaaag tgctttacca aatcgtccat ttacagcact gttgcacttt tgtcaggaaa
    18241 accaaatctc tcctggagga agcgcagatc tcttgtctgc aagcttgttc atgctgaaga
    18301 tagaggaatt atggactgcc gacacataca tttgtccgat gttactcaat gactaaataa
    18361 gacaaaattg ctaaaaaaac agtttatgca tctggttatt gactcttcct tttagcaagg
    18421 ctatcataaa aatagttttt tgtaataaaa gataacgagg aagacaatga aaaaaatcaa
    18481 ttatttagca atcattttgt tagtgggcct atgtttgttt gctctgccga cccaaggatt
    18541 cgctgtcgcg ggtggtcatg gtggaggtac atctagcggt cgaagttcgg gcagaggttc
    18601 atttggtggt cgttcatatg ggggcagtta ttttggcagt cgttcttacg gcagagattc
    18661 ttatgggtat tatggtccag gtacaggtcc ttctagttat gagcaaacgg gatttcttgc
    18721 attagctgtt ttgtcagtag gaggggtttc tgctttttct atcgtcaaga ataaaagaca
    18781 aaagaaaagg ctgaaagcgg agatattgtc ccgcatgtca ggaacggcta aagaaaagaa
    18841 agcacagttt ctggaaatcc aaaagatatt catgaagatc caatccgctt gggaagaaga
    18901 aactcctgaa aaagccagaa gctgttatac agacaagctg tttgccgaac atcagaaagt
    18961 tctgcaaaaa aacaaagcag acggtgtaag aaatcatact aaaaaaataa ctattcagga
    19021 agtaggaaat tatcgacaga tccacgaaaa tagttttgcc atccgaattg atttcagatg
    19081 ctgtgattat tcagtcgatc gaaaagatgg gcgattgatc tcaggaagta agacacaacg
    19141 acagtatttt tctcagatat ggtattttga tttttctgag aaagaaaaaa gatggcaagc
    19201 cgattttatt cagccgatta tgctggattg acaggaaata tttatgaacg gagttgtgac
    19261 aaaagccttg tcgcagctcc tttttctgaa taaacggtgt gccagagata gcctatcggc
    19321 aataaggcac gattttgtaa aatatgggaa gcgatttttc aaaatcgctt cttatcgctc
    19381 aaggctgaac atctctgtca caacctctcc actaaatggt gtttaaaagc ttgcatctgc
    19441 gataataaga acaaccaaac gaaaaatagg aagcactatt ttagtttagt tgttcttatt
    19501 acttgatgca ggacagcttt ttcacaacct cttttttgta tagatgggca aaatttttgt
    19561 aataataggc gtatgtcttt ttcgttgagg agatgaaaaa gcatatggct tattttttgt
    19621 ttgctttgct tactttcttt ccacttcaag tggcagaaat atctactttt tattatgtaa
    19681 gcggaaccat atacttgaaa tcagaaggag atcatttcta tgaaaacaag aacaattgaa
    19741 atacttcaca gaatcatgac agcccaatat ccagtaaaag caaaacatct agcaagtgag
    19801 ttcaatgtca gtcagcggac gatccgtcag gaagttcttg aagcgaatat ctggttgagc
    19861 agtaaaaagc ttccggagat ccgtacaatt cgcaacaaag gttttttgtt gaagctggaa
    19921 aaggaaaagc aagctcgatt agaaactgaa ataaaagatg tgaaagacgg ctttttgaat
    19981 cggcgcgaac gtatttttga tctagtttta gccgttgctt acagccgaat cccgatccat
    20041 ctaaacaaaa aagaagaaga atttcaaatc tctaaaagta cattagatga agatatgaga
    20101 cggttacgag caacactggt cagttatggg atagagatcg tcagttatgg aaagcaagga
    20161 ctggtttata aaggttcaga acgatctatc cgtacgatga tctatgattt tatcaatcag
    20221 aatctgggaa tgaccgactt ctttatgaat caagatgcac aattaacacc ggctcaaaaa
    20281 atctttctcc attattttcc tttggtggaa atcaaaaaaa tacaggagat ctattccaat
    20341 aagttgttga aacaagaaga tgatatttat aaaaaacaaa tcctgctttt tacattgatc
    20401 tggatccagc gaatcaggaa acatgaattt atctctgcca ttaattggaa aaatacagaa
    20461 ataaaagaca gtagcttttc tgcatttatt gaccacatga tcgaggaatt tcaattggac
    20521 aatgtcccgc aagtagaaag aaattatctt cgtttcacta ttgagacgtt caatgctaga
    20581 gatatctcca attcaatcga gtgggtacaa gctcaattac taacgatcca attgatccaa
    20641 tttgtagaga acgagacaca cgtacctttc catctaaagg aagaagcttt attcgaaaat
    20701 ctctataagc acatggctgc actgattgta agaatcaaga atcatatcca agtcatgaat
    20761 ccattgaaag aaaacatccg aacaaattat ttcccaatct accgcgcagt cactcatttt
    20821 gttcctacga tcgaagaagt aattggtggc aagatactgg aggacgagat agcttttctt
    20881 gtcatccact tttcgacgat cgccagtgta atcaagcgag acttgaatta tgtttataaa
    20941 tcagtcgttg tctgtaacca tgggatggct actggaaatc tgttagcaga gaatctgaaa
    21001 gaaaagtttc ctcagatcga gattactgca gttttaagtt caaaagaaat cgaattagta
    21061 gataaattgg atgtcaatct ggttttcagc acctttcaat tgaattatcg aaataagccg
    21121 cttttagttg tcgatccgat tttaactgag acgaatatct tggttataga agactttttg
    21181 aaaagaaatg aagaactaca gcggttagag cctaacagtc atgaatcgac ggagttattc
    21241 actcaaataa tagatgtcat gaaagaaagt ggtggccgag tagatcgaat catctatggc
    21301 aagttagaaa aattattcga aacaaacaat ttagaaatca acaaaaggga gatccagcct
    21361 atgttaaaag atattttgac ggacagcaat atcatgatca aagaaaaagc cactacttgg
    21421 gaggaatcga ttgaactagc tgctagacca ttggttaaag aaaacattgt cgaagagcgc
    21481 tatgtcgaag cgatgattcg tgcagtgaat gattacggac cgtatatcgt gattggcaaa
    21541 cacatggcac tagcccatgc ccgaccagaa gatggggtca ataaattagg agtcagcgta
    21601 gctacgattg agcaaccaat cgatttcggt aatcctgaaa tggatccagt aaagattatt
    21661 ttttgtttgg cagcagtaga ctcctactcg catctgaaca tcatgaaaga actaattgaa
    21721 ttgatcaaca acgaagagaa gttggatcga ttgattgaat gcacaaatat cgaagatttc
    21781 aaacaactgc ttttttcagt agttgagaaa taataaatga tacgggggag aacaaagatg
    21841 aacaaattga acattttatt cgtatgcggt gcaggtttag gaagtagttt tgcagcacag
    21901 atgtctgctg aggatgtttt gaacaaatta ggagtagaag ccaaactgga ccattgtgat
    21961 atttcttccg cagtttccat gaaaccagat gtgatcatca cagcagaaaa ttttcgttcg
    22021 cagttcgaaa agttcgcagt tgatcctaaa acggcgatga tctatttgaa aaatattgtt
    22081 tcaaaaaatg aaattgaaga aaaaatcacc ccagttctgc aagataaggg agttatctaa
    22141 atacaaaaag aaaagagggt aaaaa
//
LOCUS       DAYYGS010000049        21946 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000049 DAYYGS010000000
VERSION     DAYYGS010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.049"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..569)
                     /locus_tag="ACHA44_001709"
     CDS             complement(<1..569)
                     /locus_tag="ACHA44_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8904981.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAV"
     gene            complement(718..1431)
                     /locus_tag="ACHA44_001710"
     CDS             complement(718..1431)
                     /locus_tag="ACHA44_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8904982.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            1606..3030
                     /locus_tag="ACHA44_001711"
     CDS             1606..3030
                     /locus_tag="ACHA44_001711"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8904983.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            3329..4036
                     /locus_tag="ACHA44_001712"
     CDS             3329..4036
                     /locus_tag="ACHA44_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFM8904984.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            4023..4346
                     /locus_tag="ACHA44_001713"
     CDS             4023..4346
                     /locus_tag="ACHA44_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HFM8904985.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(4435..5472)
                     /locus_tag="ACHA44_001714"
     CDS             complement(4435..5472)
                     /locus_tag="ACHA44_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8904986.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(5793..6776)
                     /locus_tag="ACHA44_001715"
     CDS             complement(5793..6776)
                     /locus_tag="ACHA44_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFM8904987.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(7191..7961)
                     /locus_tag="ACHA44_001716"
     CDS             complement(7191..7961)
                     /locus_tag="ACHA44_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8904988.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(7954..8889)
                     /locus_tag="ACHA44_001717"
     CDS             complement(7954..8889)
                     /locus_tag="ACHA44_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8904989.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(9205..10854)
                     /locus_tag="ACHA44_001718"
     CDS             complement(9205..10854)
                     /locus_tag="ACHA44_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFM8904990.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(10971..13574)
                     /locus_tag="ACHA44_001719"
     CDS             complement(10971..13574)
                     /locus_tag="ACHA44_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFM8904991.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(13586..14635)
                     /locus_tag="ACHA44_001720"
     CDS             complement(13586..14635)
                     /locus_tag="ACHA44_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFM8904992.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(14661..14975)
                     /locus_tag="ACHA44_001721"
     CDS             complement(14661..14975)
                     /locus_tag="ACHA44_001721"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFM8904993.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(14991..15437)
                     /locus_tag="ACHA44_001722"
     CDS             complement(14991..15437)
                     /locus_tag="ACHA44_001722"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFM8904994.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(15438..17360)
                     /locus_tag="ACHA44_001723"
     CDS             complement(15438..17360)
                     /locus_tag="ACHA44_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8904995.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(17572..18468)
                     /locus_tag="ACHA44_001724"
     CDS             complement(17572..18468)
                     /locus_tag="ACHA44_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8904996.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(18461..19141)
                     /locus_tag="ACHA44_001725"
     CDS             complement(18461..19141)
                     /locus_tag="ACHA44_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8904997.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(19142..19924)
                     /locus_tag="ACHA44_001726"
     CDS             complement(19142..19924)
                     /locus_tag="ACHA44_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8904998.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(19917..20834)
                     /locus_tag="ACHA44_001727"
     CDS             complement(19917..20834)
                     /locus_tag="ACHA44_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8904999.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(20998..21252)
                     /locus_tag="ACHA44_001728"
     CDS             complement(20998..21252)
                     /locus_tag="ACHA44_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905000.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWKTTQPRLRDSLIFLGGSLA
                     IVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEKKESGN"
BASE COUNT         6530 a         4508 c         3626 g         7282 t
ORIGIN      
        1 acagcgggag gaatatttcc ggatagctga atctgtagtt tcttattatt agaaagttta
       61 ataaataatt ctgtagccgc taatccaaca aatataccta caaacattcc tgaagctcct
      121 gtataggata taggtatcac acccgtcact tcaacagctt tatccgcatt ttcaggagta
      181 aacatcatct gcataggtgt taatacaaca aatgtcgaaa tagcaactaa taccgctgca
      241 atatgatttg tataatttct gcttgcacac atatgataag atactgctgc tacaactaat
      301 aatgtagtta tgcttaatgt accattggca attgaagtac caatttgttg taacgtggtc
      361 aatgtactag tatcgacaat ttcccccatg aatcctgttg gttctaagat cactgaatta
      421 ataagtgtca caaatcctgc taacatcata aatggcataa atgtagcaaa cgcatctcgc
      481 aaagttctta aatggatctg attccccaac ttagtcgcaa ctataccaaa tttttcaaat
      541 acaatgtcct tcctgctact tttgcccata ttttttctcc tttatgctta ttgtaatcga
      601 ttactttttc cacccaaata atacatcact tatcaaaaaa agtaaatact tttaattaaa
      661 tatatatatc ttttttagta taaaaaaata agctctgaaa aaataatttt cccgagctta
      721 tttttcaaca aaaacagctt caaaaacaaa gtcttgatat aaatgtctcg ataccgaata
      781 ctcgaaaggt ttaccagacg atagagcaac aactttgtct atctcaatca taaattcacc
      841 tggatctatt tttaatattt ttgcgtcaaa ttcattcgca gtatctcctt tgatacgtat
      901 gtgcgaagtc tttatttcca gacccagttc atttgtgata taagcatata cagatttctt
      961 taaatgtttt ggttccaagc ctggtatgac tgctaatggc atgaatgttt gctcaatcga
     1021 gtatggttga caatcaataa aacggcttcg tacaatatta tatacatagt cttttacact
     1081 gatacttaat ttatcagcta cttttttcga tggaagttca attgagaaag tatggatttc
     1141 tgaatctacg tgttcttcac caaccacgct aaacaaacct tcgtttggac caagaactcg
     1201 tgagttatca attggtgttc tgacgacttc agtcccatac ccacttctac ttcgcaaaac
     1261 tccttcggaa acaagtaagt ctacgccttt ctttactgtt aacttgcttg tatgataaat
     1321 aactgccaat tcatctcctg taggaagttt ctcaccaatt ttatactctc catttacaat
     1381 ttttttcttt atatctaagt aaatttccat atacttcgca atttttttca atggtgaccc
     1441 tccaatcaaa aacatatctt catctatact ttaacataaa taaaccgata aaatccacat
     1501 caaaattata cacacaataa aaaagatata tatatttaaa caaaactatt tactttaagt
     1561 tgtaagcggt gttatcatta agtggaaaca ggaggtaaaa aaattatgac aactatgaaa
     1621 aagctaactt ttccagatga tttttggtgg ggttccgctt ggtcagctga gcaagcagaa
     1681 ggaaaaggag aaacaggaaa agcagagact atctgggatc gctggtttaa agaacaacct
     1741 taccggtttt atcaacgaat cgattcctct attacaaccg atcacatcca tcaatataaa
     1801 gaagatatag aactaatgaa aaaaactggg cataactcct ttcgcgtttc tatttcttgg
     1861 gcacgaatgt ttccaaatgg cggtaccgga aaagtaaatc ccaaagccat ttctttctat
     1921 cgaaaattat tttccgaaat gaataataac ggaatcaaag tatttgccaa cttatatcat
     1981 tttgacatgc ctgctactct tcaagataaa ggaggatggg aatcacgtga agtagttgat
     2041 gcttatgttc attacgctga aacttgtttt aaagaatttg gtgacttagt ttgtcattgg
     2101 tttaccttca atgaaccact tgggcctatt ttaggtactt atcttgaaga ttttcattat
     2161 ccaaacctca ttgactttaa acgcggagcg caagcagcat tcttcactat acttgcacac
     2221 gcaaaagcaa tacaagtatt taaagaacta gctcttccta gtaaaatagg cgttattcta
     2281 aatttgagtc ctacttttcc tcgtagtcaa aatcctgctg atgtgaaagc agcagaaatc
     2341 gctgatttat tttatacgcg gagcttttta gatcctatgg ttaaagggaa tttttctaaa
     2401 aagcttatcc tgttattaca agaatataat cagcttcctg aagattatac gaaagaagat
     2461 cttgtatgta tctacgaaaa taccgctcaa attcttggac taaactatta cgagccacgt
     2521 cgcgtcaaat ctcgttcttc tatggtggat acatctagtc catttttacc agagtggttt
     2581 ttcgaaccac atatcatgcc tggacgacga atgaacgaat accggggatg ggaaatttac
     2641 gaaaaaggaa tttatgattt gtgcatggat atcaagaacc attatggaaa catcgaagca
     2701 tttatctcgg agaacggaat gggcgttgcg gatgaagaac gtttcatgga tgatacaggg
     2761 caaataattg atgattatcg gattgagtat atcaaagatc atcttgctta cttatggaag
     2821 gcaataaatg aaggatgtaa cattaagggg tatcatcttt ggacatttat tgattgctgg
     2881 tcatggatca acgcctataa aaaccgatat gggctggttt cactcgacct tactactcaa
     2941 aaaagaacaa ttaaaaaaag tggcgaattt tacaagcaat taagtgatac aaatggattt
     3001 agatacgatg tagacttgtt agttgaataa tattctaaaa ttccacttga agtgaacaca
     3061 ttagaaaaca agaataatga gaaataccaa taacaaatac tttttcttca tacttcattt
     3121 atacttgtca gtataagaga ctgtgacaaa tgtagtgatc ctcagaagtt aggtctggaa
     3181 atctaacttg agaagatttt tttaaactac gtccagtctt ttttatttgt ttaaaattga
     3241 agttgacaag tcttcattaa aaatttagaa tattcacaag tttgacgaca attatgaaaa
     3301 tttatgtcgt gctaaaggag ttttgttatt ggatgaaaag ttagatataa aaactgcgat
     3361 caaagacacg ttacctaccg tttttggtta tatcggtatt ggacttgcat ttggtatcgt
     3421 tgggaaagct gccgggtttc atccgctggt cgtcacgttg atgtccctgc tgatctatgc
     3481 tggttctgcc caatttatca cggtcagcat gcttgctagc catagtccac tgctctccat
     3541 cgttctctcg acctttctag tcaattcccg aatgattctg atgagtatga cgattgctcc
     3601 ttatttcaag aaaaatcggc tgcttcaaaa tctgctgatt ggcacattgc tgacagatga
     3661 aagtttcgca ctgggaatga ataaattgaa ctatactgga caaaagttaa actttcgttg
     3721 gatgaataca gctaattgga tttcttattt gacgtgggtg ggttcttctc ttgttggcgc
     3781 acttttaggg aattttatta ctgatccgaa aaagtttggt ttggatttcg ccatcgtcgc
     3841 tatgtttatc gggttacttt atcttcaagt catttctgat agaaacacct ctaaacgatt
     3901 acagttgatc ctgattggtc tcactttgat tctggtatat gtcggactga tttttatccc
     3961 aagtaactta gtgattttag ttgtaacact gattgggtgc ggattggggg tgtggatcaa
     4021 acatgccttc ttttgagttt acattgctga ctattttagg ttgtacttta gcaacttggc
     4081 tttctaggat cctaccattt gtcttactga aaaaattcga tttgccgcaa ccgttgcttg
     4141 agtatttaag ttttgtcccg atcgtcatta tgtctgcttt atggttcagc agtctattta
     4201 cccaaaatat cggacatctg ccgcagatca atctcgaaaa tgctttggct tcagttccta
     4261 cacttttagc tgctattctt tcaaaaagct tattggtgat cgtcttggta ggaattcttt
     4321 ccctttcgtt gattcgctta ttataaataa aaaaacaaga gcttatggca aattactgtc
     4381 atgcgctctt gtttttatca aagtcaaata agaaaaaaat cggcgtattc ttgcttatcc
     4441 gttgaacgga ccactaacaa cgacttttcc taatatatgt cctgtttcta cgtctttggt
     4501 tgcttgtttc aagttcttcg cattcatccc atctgaatag accttgctca atgtactgcg
     4561 gataatccct tcatttgcta aagcagcaat gtgcgctaaa gcttctcctt gcgtttcgat
     4621 ttcatagtta tagtctgttt tagcgaacat atactcccag tcgaagctga tagattggtt
     4681 tttccaaaca cctatatcta acggctcttt gatccctacg atcgttccca catgtccaaa
     4741 tggtcggaca aggtgtttga tttgatcaaa gtagcgggta atatcgaata atacagcgac
     4801 atactctact tgatcgattc ctaattcacg aaaactttta cttaagtcgt gatgataatc
     4861 gattggatag tctacacctg tttttttcag ccattcaaaa ttcttcggac ttgcagtcgc
     4921 gtagacttct aggccagccc aatgcgctag ttgattcaaa atcgaaccga cgcccccagc
     4981 tccgttgatc actaagattt ttttcccgcg gttggcattt tcttctggag tcagaccaaa
     5041 tttttcaaat aacaattcat aagcggtcaa tgaggtcaac ggcaaggctg cggcttcttc
     5101 atctgaaaga ttttttggtg caagagcgac aatacgttcg tcaaccagtt ggtattcttg
     5161 attgcttcct gctcttgtcg ttgtacctgc gtaaaaaacg cgatcgccga cagaaaattt
     5221 ttgcacttca tcacctacag caacaacttt cccgactcca tcaaacccta agatacgtaa
     5281 agcatttctt atcccgttat cttggcgaag tttcgtatcg acaggattaa cagatactgc
     5341 gttgactttt accagaagat cccgtgaact gggtacaggt atctgttctt tttcatcaat
     5401 aaagcttttt ggatcatcga tcgacaaccc ttcataaaaa ccgactacct tcattaattc
     5461 cttttctgac atacgaattc ctccttagct tcatcaagtt ggagcatcca tacagttttc
     5521 atttcaatat atatagtatg ctatttgttc cacttttatt catcataatt attctcttac
     5581 ttaaagtaaa ctatttgcac ttttttggtt aaaaaagagg ttgcgacaag acttttgtca
     5641 caacctcttt taaacggtgt tcaatcaact gaccctcggt attcgctcaa aaaacgacaa
     5701 aatatgagga gctatttttg tttggctgtt cttattactt gatgcaggac agctttttca
     5761 cgacctctaa ttcacggtgt tcaagcaaga aatcattgtt cactgaaaag cgttggatgt
     5821 tctttttcaa aggtgtctaa gatctctcgt acttcatcag tagatttcgt attcatcaac
     5881 tgaactcgca agtcatttgc tcctggaaat cctttgatat agattttaaa gaaacgatgt
     5941 agtcctgtga tggaacgcgg gatgatttct gaataatgat cttgcagatc tagttgcaga
     6001 cgaagtagtc cgatcagttc tttttctgta tgttccctcg gttctttttc aaatgcataa
     6061 ggattcttga aaatcccccg gccaatcatg atgccatcta cgccatattt ttcggccaat
     6121 tcttctcctg tctggcgatc tgggatatct ccgttgatcg tgatcagtgt ttgcggagcg
     6181 atttggtcac gtaaagcaat gattttaggg atgacttccc aatgtgcatc tactttactc
     6241 atctctttac gcgtacgcaa atggacagaa agattggcta tatcttgatg aagtaggtga
     6301 ctgatccatt cttccatctc agacatttcc gtatagccga tacgcgtctt gacgctgaca
     6361 ggcaaaccgc cagctttagc cgcttcaatc aattgtgccg ctacttctgg gcgaaggatc
     6421 aagccactgc cttttccacg ttctgctaca ttagggactg ggcagcccat gttgatatcg
     6481 attccttgga agcccatttc tgccatagca atgctcattt ctctaaaaaa ttctggcttg
     6541 tctccccaaa tatgggcaac catcggctgt tcatcttctg taaaggtcaa tcttcctcgt
     6601 acactgtctt ttccatctgg atgacagaaa ctgtctgagt ttgtaaactc agtaaagaag
     6661 acatccggtg ctgctgcatg ctttacgaca tggcgaaata caacatctgt tacatcttcc
     6721 attggtgcta aaacaaaaaa aggctttggc agctctttcc aaaaattagt agtcatttat
     6781 ttgttactct ccattctatc aagcagtgaa gtcgcaatct ttctcaaaaa cattgtcatt
     6841 atactaggaa acaaaaaaag aagcaaagtg aacagcttaa aagatgtttc tgattgagca
     6901 aaaaaagact gtgacaaaaa tcttatcaca attccttttc cggaataaat ggtgttcaaa
     6961 agcttgcatc tgcggtaata agtccaacca aacgaaaaat aggaggagct atttttgttt
     7021 ggctgttctt attacttgat gcagaacagc tttttcacaa cctctccact aaacggtgtt
     7081 caatcagctg accctcggta ttagctcaac aaacgacaaa atatgaggaa ctattttccc
     7141 gtttgtttct ctaatactca ggtcaaaacg ctgatttcac aacctctttt ctaacttctc
     7201 aatcctcttc ctttattgat caaataccaa gtaacggcgt acaaaacaac gatgaatagc
     7261 acaagtacga tcatcgatgt tgctactggc acatcgattg ttccaaggaa tccgtaacgg
     7321 aaaccggaga tcatataaac gatcggattg actttcgaaa cagcttgcca aaatggcggc
     7381 aacatagaga tggcatagaa aacgccacct agataagtta acggctgcaa gacaaatgta
     7441 ggcacgatcg atacatcgtc ataagactga gcaaaaatcc cgttgagcag tcctgctaac
     7501 gaaaacaaga ttgccgtcat caataatgta atgatgacca tgaaccatga atacacatga
     7561 agtggtacaa agaacaagga aatgatcgtt acgagagagc cgacgagaat actgcgaccc
     7621 actccgccaa tcacaaagcc ccaaatgatc acatgcgtag gaacaggcgc taccaagatt
     7681 tcttcgatgt ttttctggaa tttttgagag aagaaggacg aagaaacgtt ggcatacgaa
     7741 ctagtgatag cagacatcat aatcaaacca ggcacgatga attccatata tgaaaaacca
     7801 cccatgtcac cgatccgacc accgatcatt tttccgaaaa taacaaagta caaggatgtc
     7861 gtgatcacag gcggaaccag tgtttggacc cagatacgca aatagcggtt cgtttccttg
     7921 atagctaaac tttttaatgc agtaaaataa agattaaaca tttttctcct ccgtatgatt
     7981 attttcttct gtgattttca aaaacagttc ttctaaacga ttcgacttat tcctcataga
     8041 aagaactttg attccttgtc ttgtcagctg atcgaagatt ccgttcaccc cttgatttcg
     8101 ttcaacttcg acagcgattg tctgttcatc ttcaaatgta caagcgtatc cttcaatcac
     8161 aggtttggtc tcatatggcg ctaaatcaaa gataaatgtt tcaaattgca gtttggatag
     8221 aagctctttc atactagtat tctcgatcag ttcacctgat tggatgatcc cgatgttgcg
     8281 acaaagcatt tcagcttctt ctaaataatg cgtcgtcaaa atgatcgttg taccattttc
     8341 gttcaattct cggagaaact cccacatctc gcgtctcaat tcaatatcga ctcctgctgt
     8401 tggttcatcc aagatcagca aacgtggttc atgcatcaat gccctagcaa tcatcagtct
     8461 tcgcttcatt cccccggaaa gcatacgtgc acggacattt ctcttttccc aaagattgga
     8521 ctgcttcaaa tatttttcgc tgcgtttcat cgcttctttg cgagagacac cgtagtagcc
     8581 tgcttgattc acgacgattt gctggaccgt ttcaaaggga ttgaaattga actcttgcgg
     8641 gactaatcca atctgttgct ttgctcggac tagatccgta tccagatcat aaccgaagac
     8701 attcactttt ccagatgttt tattgactaa agaagtaatg attccgatcg ttgtagactt
     8761 cccagctcca tttggtccta atagcgcata gaaatctcct tctttcacgg aaagatcgat
     8821 tcctcgcaat gcttcgaccc cacttccata aacctttttc aaattattta tttcaagcgc
     8881 atttgccatt tatttcctat ctccttatag acatgattct ttattgtatc atggaataca
     8941 gcagatgaaa aaacaacttg gttccagcga aaaatcaggt tgctgggctc aggaaaatct
     9001 cctaatgaaa gacatcaatc ggacgaagtc agacaacctt ttcagaaaaa acaaatcgaa
     9061 gataaaggat tactattcat atgtgcaaca aaaggagcgt gagacaaaag taaagaacac
     9121 ttttgtctca cgctctaaac acgtataaac ggcggaagca aaagccaaga tttttgatcc
     9181 aataaatact ttcgatcact tcgcctatcc caatacatgc cactgccgct tttcatcaaa
     9241 taacgcggct tccgtttttg cggtttctac taaagtctct gggtaatcca aaactttcag
     9301 caaggcaatc gcatttcggg tccttgaagg tccttctttt aatttgaaat caaaactgat
     9361 tccttttcct tctgttactt gttcttcaaa gtggacattc tcacaataat tctttaaaat
     9421 ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa gatggatact ccgcaagcca
     9481 ccgaacgact gaagcggaag aagcaatccg ttcgatcgta tttgtccctt ttaaaatctc
     9541 atctacgaag cagtagcagc gttctttgga agccacttgg tccaacaaac gtttgatcga
     9601 tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa tcatcttcga ttgccatgga
     9661 agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca atcgctgttt ggatcgtctg
     9721 tgctagaata cagctgatgg ctatactttt gacatacgta gattttcctg atgcattgga
     9781 acccgtgact aatgtattct tctgccagtt gaccggattc actaccgctt cttttaacaa
     9841 tggatgatag atatctttcg ctgtcacacc gccctcagta aactctggtc ggcaagtgat
     9901 gggcatataa gtacggtaat tcaatattgc agcggctact tctaattctc ccagcaattc
     9961 ccacagcttg atggcttcag ttgaatgctt ttctaattta gtgagaacga aatgatagga
    10021 gataaatggc agcatgaaca tgatgttgac atattcaaaa atcaactctg cttcactccc
    10081 actcttcata cgaaatgaaa aacctaattt agggatgtct cttaatggtt tagtttgctg
    10141 tttcagctct tcttgaagtg gtgtcggcag ttttgctagc tgattggcgg ctgaaatcgt
    10201 tgaaaccaaa taactcatgc tattcagctc tgtttctagt gtttgtttct tcactagata
    10261 atagaccgta ttgaatacta acccaccgat tgccaaatag attcctagta cttgtcctaa
    10321 aagaagcaaa cccaaaccca ccagcggaag caagccaaga aaaataaata aacctacgcg
    10381 tccaatcttt ttactttttc catccgctag atattgtttt gcaaaattat gatctttttt
    10441 tcctaaacga gcgaattgat attggatttt ttctctcagt tgaggatttt cctgataaaa
    10501 agcaatcagt ttttctaact gctgatcttc accaaaatca aagctgcgta aacgttgata
    10561 caacgcttct gaacctacac tagaataggt actgttgatt ccttcgaata cttcgaacat
    10621 atccaggtca taccaagtaa gatcatcgat ttcgctatcc catgaacgga acttcttctc
    10681 cgtgagccat gcttccttca agctttcttc tttatcaaat ctcggttgat aaggaatttt
    10741 ttcccatttg ctacgaactg tttcctttaa tttcaaacgt gaaaaaatgt ctaaagcaac
    10801 taataaaata acagctgcaa taataattaa aacgataaat tctctttgac ccatatccct
    10861 ctccgttcct ttatttgtta gctttagtat agcactgttg cgctatttga aaagagggta
    10921 aaaaaacacc tttcaaaata aaaggtgttt ttttcttgta tattacatat ttatctctca
    10981 tttatcaaaa ttgacaaata atcataggga ggaattgttg ttaattcttg tttattctcc
    11041 agtgcattga caactgtccc aagggaaagg atagcctcag gtactatttt ttcttgttct
    11101 gttggattag ctaagcgaag aacaaatcct tgttcatgaa aataagaagg ataaatcgat
    11161 gatacaatta agtcttcagg taattcaatc aatgaaaatt tacgaggaac atcaagttca
    11221 ttttgtaatg gccaaatctt gttatctaac ctgtgaataa atacattcag tttctgcttt
    11281 tgataagaaa tagattgttc taaccgtttt tctgcctgtt ttgccacttt gaactcatta
    11341 aatcttcctt cttctacatt aaatccaaaa gtgactttat actcacctat ctcttgagct
    11401 agcggtgttt ccatcatgac atgcccttca tttgtcgtat ctcctgaagc tcgcccaggt
    11461 cgccaagcaa gattaggttt tccaagttcc cctgttgttg aaaataaggt tatgattaat
    11521 tgattttcta tacgttcata ctcttttaaa ccatctgcat aaaacgtaaa ataatgatca
    11581 ttttctgcca cagagacact cttatcgaat atttctaaat taacaggttt ctctaccata
    11641 gtttctgacc aattcggagg aatgacatca ggggtatttt caataaatcc gttttgaatt
    11701 tgtgcaattg atttttcaga tttatctaaa acattaattg ctaagcgcat acgatgcgag
    11761 taaacagtat tatgaatttt gagtgtgcct tcaacgattt gactttctcc cagactgaac
    11821 gatatttcat aagatatttc ttccatttcg ggtttttcag ataaacgatc ttttaaatct
    11881 ttaggaagtt gagcttttcc gtaaacaact aatgtttctt gtaaagaatc tttatatact
    11941 ttagctgttt ctaattttag agatagttct gtatctcctt ctaatggtga ataatcatac
    12001 gtatcgccat catttgctga atcgatcaaa tgaacgaaat caagatattg tctatctcca
    12061 actatcaaat tcaattgccc atctttaaaa gaaagtttat atttattgtt acttatctca
    12121 gttttgtcta tctcgattgc tgtttctaat ttttcattat ttttctcaaa ttcaacaaca
    12181 gtgtatccta gtgctggcac atttacattt atttttatgt caagttcata ataagctggt
    12241 tcatcaaaat attcgaaacc tgtagcaatc atccgataag catttttccg ttctggaaaa
    12301 taaacttctt tttcaatgat attataggga ctacctttga agtgtatttt ctttgaatca
    12361 gctactacat gcacgatttt cgatccacta taattctcag tcgttgtgtt gaaaacaagg
    12421 acttcattcg attttagctt taatgcatca gccatccgtt ttacaataag gttttcgatt
    12481 ccgtcagcta tctcattagc ttctttgatt cgatggaaaa tatcttccgc aacattatct
    12541 gaaacacagc caccaatact atcatgagcc tggttttgca aaatcttttt ccataaccga
    12601 cgaagtaggc ctttactgat atggatacca tttctttctg caatcagcat gagcggctca
    12661 actcgtttta gaattttttg ttccaattta aaattttcta ttttcatttt tgttcttact
    12721 gagccaatag aacgatgaac acgagagtaa accggctgac gaagttcacc tttatattca
    12781 ggtaattctt ttttgtttct gataacttct acataagaag gataatctga aataatattg
    12841 tggaatttac tgtatttatt caacttctct acgacttgat caaaatttaa aatcattgac
    12901 ttttgatcta cacccgaagg tactaaaata tctttgtttt ttccttgcat atgaagcatt
    12961 tcaactgctt catccaactt ttcttttgca aattcaggaa tatctttaat tgcgtctaag
    13021 gtcatcattc cagtcatata accaaatgga aaattcatgg cataaacaaa tttatctccc
    13081 aatcctttcc agataaaata aggagatgga accatcgttt caaagtttaa tcctcgccaa
    13141 cacatgaaat tatcgatttc tacttgatgc aaaagtgtag gcaaattcgc attgaaccca
    13201 aacgtgtcag gcaaataccc taccatcatt ggatctccat atttaaggat cgtatcactg
    13261 attccaatca ttaagtttct taaaattgat tcagcatcca ctaataaggc atctgtttgt
    13321 gcataccacg gtcccacgaa tagttgtcct gttttcatca gtcgttggat aaccttaagc
    13381 ttttgcggat tgatctccaa atactcatcg acgattgaag tttgtccatc taaacaaaag
    13441 tttactttag ggttcctttc caattcatct agaacttcag taaagacctg atcagacaat
    13501 acgatggcat cttcatttgt aaaataccat tctctgtccc agtgcgtgtg ttgaattata
    13561 tgtgcattta ccatatcaga ttctcctact ttccttcttc tactgaattt acttgttgtt
    13621 cttttttata cttgatggat tttagtgcat tgaccattaa cgctgtaacc actcccccaa
    13681 taacaattga tactaaatat aaaggtatat tcgtaacaaa aggaattgcc ataaaggttg
    13741 aaagcattgt ttcttgagta acaccaaaaa atccatttag accacatgct actgcagtgc
    13801 ccaccacgat gcttggtatt actcgtaaag gatctttcac ggcaaacggg atcgtccctt
    13861 cagtaatccc cacaaggccc ataatcatag ctgtctttgc attatctcgt tccgattcat
    13921 cataaagttt tttgtttaaa attgttgcta aaaacattcc aaatggcgga atagcaattc
    13981 ctaataacat ggctgtacta ggtccaaagc ttccgcttga aaaagaagca accccaaaag
    14041 catatgcgat tttattgata ggtcctccca tatcaaacgc catcattcct cctaaaatag
    14101 ccgacatcaa agcagcattt gctcctgaca tgccttctag ccaattagtc agtcctgttg
    14161 tcattgcttg aataggcgta gctagtacat accataatat gattccaaca actaatgtag
    14221 taacaaaagg aatcagcata attgatttta gactaataac ttgcatcggt acttttactt
    14281 ttttcaatgc attagctaaa tagccacaag ctaatccagc aaccattcct ccaataaatc
    14341 ccataccaag attttgacac atcatcccag tgataaaagc tggtgctaga ccgggacggt
    14401 cagcaattga atatgcgata taggctgaaa tcatcggtac gataaactgt aatcccgtct
    14461 gaccaatcat tcgcaggact ccccagaatg tatccgtctg gtcccaaaca cctgtgccac
    14521 caaaaatgac tgcaatagaa gttagtaacc ctgctgctac gacaagcggg atcatatagg
    14581 aaaccccact ttggaaatga tttagaattt ctcttccttg tttcttgaat tccattagta
    14641 actcccccac gatatatttt ttatttgtta attaatgcaa ttgcttcatc aatcgtctct
    14701 tcaccatctt ttactacctc tgaggtccct actgttaaaa tcggtttacc gtcaaagcga
    14761 cctcgttctg aaacatttgt atctactgca aatatgacaa catctgcctc ggaaacatcc
    14821 gaaattgaaa gtttgttttc tatccccata gccccctgtg tttctacttt aatatctaac
    14881 cctttcttct tcgcaaactt ttctaaatta gcttttgcca tataagtatg ggcaatccct
    14941 gcgatacatg ctgttactgc tactattttt gtcatatgaa attcctcctt ttaaaatgat
    15001 agagctttta ccaattcttt tttatttctc gaatctttta ttcttttaac aaactcgtca
    15061 tccatcaatc ttccagataa ttctgctaaa atacgtaaat gattagtacc tttatcagtg
    15121 tcttttattg ctaacaaaaa gacgagatca actggttgat catctaatgc gccccattca
    15181 acagaccgct ttaacttggc tacagccact gttgattcaa ttactgctag gcttttgcca
    15241 tgaggaatgg cgatattttt cccaattcct gtggtcatat ggttttctct gtcaaatacg
    15301 tctttttgaa attgtttgtg gtcagacaca aacccattag tctctaacaa tttcgataat
    15361 ttttcgacta cacttttttt atcagatgca tttaaatcta tcactatatt atttatattg
    15421 attttttcac tgatcattta taagccttct ttcaatatat aaactaattt ttcaaacgac
    15481 gaactcgttt ttaaatttgc taataactca tgattcgaag cgatttcatt gaatgtttca
    15541 taaattttat tcaattcagg ttttactttt tcattaagtg caaaaaagaa aacagaatga
    15601 acaagtgtcc catcccaatc aattccttct ggattaacgt agacacagat tactggcttt
    15661 tttataaaat ctacttcgat atgcggcata gcaaaatcat tcatcgctgt tgaagatagt
    15721 ttttctcttt tgattgctcc ctctagtatc ccttctttcg cgtaattttc ttttaccaat
    15781 tttttggtaa ttatatttaa aacgttcact tgattatcct ttatttggtg ggtaaaaaag
    15841 caaaatcgtt tatctaataa atttagaaaa gcatcttctg tttgttcatt taactgtaga
    15901 tactgtttta cttttctgac atctgcccct aacatcaaag gactaacaac aataacagga
    15961 aaatttgtat tttcaatcgg aatcgtgctt ataacaagtt catcagtgac ttttctatct
    16021 tgtagttcat tgatgcttag tacatcttct atctcaacca tatctgcaaa accgttattt
    16081 attctttgct ctaataattt tgatgttcca tatccgctac tacaaactaa tatcactttc
    16141 gttttgtcag gtttgtaacg atccaaaagt gattgaatat ggagtgttac gtaagcgatt
    16201 tcatcttcat taaaatgtat acagaatctc tcttcgattt cctctaataa atctacactt
    16261 atcataaatg accttttaaa agaatatttg attttgtccg tataagggtt atagatattt
    16321 actcccaatt tgagtctttt aactgctgca ttgagatgta atactagatt attgatcaat
    16381 tcttcatcag gataaaattc atttaattta attcttacta actcttgtag ctcaacaccc
    16441 atatctaaat aggaaagctt cgtttgatct aagtcgtgat tcatagcatt tttttcaatc
    16501 gaagtaatgt gaatgtcaac ataagcttgt tcatctattg gcacaataat tgaaaatgct
    16561 ttttcaatca ttttgattaa aataagtgta ttctttgaga tttcttcttc agaattatta
    16621 ctacgtgata agaaattgct tgtttcaatc ctttctatcg caatagcaag gtggatagct
    16681 aaagtttgaa attcataatc tgtaaatttc aattgtgtct tattcatgta tcgattaagc
    16741 aattcggcta cttttttcaa tatgtcctta tttaagaaat tattcgcttc accaatgaaa
    16801 ttgatattca agtctaagag atctgtttta ctgttagatg ctgtaaagcc accccaataa
    16861 tagttcacaa cttctgacat caaccttctt ttactttttt catcagcttt gatgcgcacg
    16921 ccatttcgat ctgaaactaa ggacatccca tgcttgttta gtctttttct aacatctttc
    16981 attgtgcttt ctaccgtcga cctgctcacg tataattcgt cagcaagatt ttgaatcttt
    17041 agagaatcat tagtattcaa taatttagca aacacataag ttagacggtc ctccggagag
    17101 tctgcgatat tgattttatg actggataat aaatttcgaa gtgtttcttt ttttccaata
    17161 agcgaaacgc cttttttagg ttgaaccaac atttgtacat ctggcatttt ttggattatt
    17221 tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag atacatataa ttgttctgat
    17281 aattctttgt aagttgtgtg ctcattttca atcaaaaagt atagtatttt catttgccgt
    17341 tcgctaaatt cgatgcccat tatcttcacc tcctgaatac agcttaagtg tacaattatt
    17401 atctctattt tcatacagaa gaaagttctt gattaatgta gaagtttcta atattcttga
    17461 ttatttcaaa tcaataaaaa agcctgcaat atttatatca caggcttttt tctctattaa
    17521 atatatataa gataatacat ttttctacta gttctcacta atcttgtata attaaaccct
    17581 ccgcaacttg acggagatgg agaagatgcc cccgtctaat ctagcggtca tctctcctcc
    17641 cattccttcc accaattttt ttgacaggta aagcccgatt ccgctagatt ccgtatttgt
    17701 gcggctggta ttttcggtat aaaaacgctg gaagactttt tcaacagagg tttgcgttcc
    17761 tggtttgata ggattggcca ctgttaatcg aatgaatgga tcctcttttg aaaatgtcac
    17821 ctttgcttgt ccatctgcat aacgcaataa attctgtatg atattttgca gcactcgcag
    17881 caacatgagc ggatctgcta atacagttgt atttatcgag tgttcagcga acgctacctc
    17941 gatatttttc tcagaaaatt gttcataaaa agagaaaaat acttcttcca cctctttaga
    18001 aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc tctattcgag caagatcata
    18061 aaactgatcc gtcatactga gtaatcttgc aacagattgt ttgatgatcg ttaaatactt
    18121 ctcccgtttc tccgtatcct ctacctgttc cagtaattgt acataaccgt taatagcagt
    18181 caaaggagtt ctgagatcat gagaaaggcc gacgatcgat agtctgatgt tttcttccat
    18241 ttcttgaaat gcttgtcctt tttgttcatt ttcatccaac catgtattca gtgcatctac
    18301 taggtccatt agattctttt ctcgaaattc gactgaaaga cgaccaccat attcttgtcg
    18361 ttcagaaaga gtggcgattt tcttagttaa tctcttgatt tccctttgca ggaagaaaat
    18421 aaaacttccc ataaagataa caacgagaag caacagccat ttaatcaaat ctaaacccca
    18481 ctccccacac cgtatgaata tacacagcat gtgcctttgc tagcttccct cgaagattgg
    18541 aaatatgcat attcactgtt ttctcactat caaaatatgg ttcttgccaa actgtttcat
    18601 aaagatttgc tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc agcagatcga
    18661 attctcttcc tgtcaaatgg acagccacac cattcacatt tacatccctc gactctttgt
    18721 ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg acagaagtct
    18781 gatgacgaag ttggatgccg attcgtaaca aaagttcttc gatatcaaat ggtttcgtca
    18841 catagtcatc cgctcctgct gccaacaagg aaactttatc tctttgatcg ctttttgctg
    18901 aaagaacgat gatcggaaca ttcgatgtct gtcggatttc tgctaacaat gcttcacctg
    18961 aaagccctgg aagcatgaga tctagaagga tcaaatcgac aggatatgtg cttaacaata
    19021 attttccttc cgtacctgaa taagcatcaa gaagtttgta tctctgttca agaacctcct
    19081 ccagtaagga atgaacagct tcatcgtctt cgataatcaa aatagtttcc agcaggacca
    19141 cctacttaat gtcagatttt tggaaaataa acattcctag agctgatgat accacgattg
    19201 ttactagact gaccagaatc attttcggtg cttgcgaata agttgcttgt tgcgcaaagt
    19261 ccgtatacat gagccacgtt tccgtaaacc actccgttag aaattccaag cctttgattt
    19321 ttgcgaaaac accgggaatg aaggccgttc ccatgatata aagaaaagaa agaagcaaac
    19381 tgacactggt acttctagtg atgaatgtca aagccactgg aacagataat gtccctaatg
    19441 tcagcagaag caatacagga aatactttta gaacataatt tccatagttt ttgattccct
    19501 caacttcacc aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc gtgatcaaaa
    19561 ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa taagtcattc
    19621 gattccgtcc taaagataac gtattgcgaa tcgtaccgtt ttgaaattct tccccaatat
    19681 aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc agccattttt tctaccatta
    19741 aaatccctgt tgcaccgttg atgactgtat ccgttgcttg gtttaattca ttgccgtatt
    19801 ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg aagtacgacc atcagtccta
    19861 gtgagaccca cattttacgg ctcttccata cttttatttt ttcagcagtt aatagattaa
    19921 acatgtggat tgcctccagt taattgtaag aaataatctt ccagtttttg cccaactagg
    19981 tgaataccat ccacaggaac ttttgcttct accaatgctt gattgatggc tgctacttgg
    20041 tcaattcctt caaaaagctg gataacacgg ctagattgta caaaataatc cctataacca
    20101 agagagtcga gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt tataaattga
    20161 cgagattctt gtgccaattc ttctttcgat aattctttga tcaagtgtcc ttcatgcaaa
    20221 ataccatagt gagtggcgat ctgtgacaat tcatctagca agtggctaga aagcaagacg
    20281 gtgatgcccc gttcttttac caaacgttgg atgatttctc gcatttctgc catccctgct
    20341 ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt gatcagtgca
    20401 tttgcaatag ctaaacgctg gcgcatacct aaagagaaat tctttgcttt ctttttccct
    20461 gtattttcca atcgcatcat tttcagcagg tcttcgattt ctctatccga cacaccgccg
    20521 ttcgctgctt ggatacgtag gttatctctt gcagttaatt ctggataaag agcaggggtt
    20581 tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc aaataattcg
    20641 atcgaacctt catcaatcga gatcaaaccc ataatagttc tcataaaggt agacttaccc
    20701 gctccatttt ccccaatcaa tccatagatc attccacgtt tgatttcgat tgatacttta
    20761 tctaatgctt tggcagcacc gtattttttg gttataccag ttgctttcaa gaccgtttca
    20821 tttccaaaat ccatacttat ccttctttct tattgatgag tgaagtatag ttgacgggta
    20881 taaagaaagc agccagaaat gtataaagaa agtgtaaatt ttccaattga atgtcaaaaa
    20941 aaacaatcgg acaatacgtg tctttcctat tatcaggcag tttgttatac gcttgtatca
    21001 gtttcctgat tctttctttt cgaccaaagt cgtttctctg tattcctctt cactgaaaat
    21061 ttttgcttct ttcagatcaa gcttgagtaa ttttaccaga ccggttataa ggataactat
    21121 agcaagactc cctcccagaa aaattagact gtctcgcaga cgtggttgcg ttgtcttcca
    21181 atcaaagagc gcaccaaaca gtgctaatcc aagtggactg gccaccatgg taatcgttgt
    21241 tatcgttgac ataagtcgtc cttgtatatg cgttggaact tctcgctgaa gccagaccat
    21301 ataaaaagca aaagaaaaca ttaatcctta aaaacggact tatgaaaaat aatacatgcc
    21361 gtttgggatt tacaattagc taccaaataa gaggttatga caaaagactt ttcacaatcc
    21421 cttcttccga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatttat
    21481 caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga ccacttctgt
    21541 cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt ttcccgtttg
    21601 tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag ctaaaaacac
    21661 aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa ggtaactgcg
    21721 ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg ataatcgcaa
    21781 ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt ttttcttcgt
    21841 atcattgaat aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa tatattttat
    21901 tatatttgat taaatttgaa atgtttgtgt tctaaaatgt gttcta
//
LOCUS       DAYYGS010000050        21935 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000050 DAYYGS010000000
VERSION     DAYYGS010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.050"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA44_001729"
     CDS             complement(44..1339)
                     /gene="purB"
                     /locus_tag="ACHA44_001729"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFM8905001.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA44_001730"
     CDS             complement(1352..2476)
                     /gene="purK"
                     /locus_tag="ACHA44_001730"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFM8905002.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA44_001731"
     CDS             complement(2469..2957)
                     /gene="purE"
                     /locus_tag="ACHA44_001731"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFM8905003.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3373..4680)
                     /locus_tag="ACHA44_001732"
     CDS             complement(3373..4680)
                     /locus_tag="ACHA44_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HFM8905004.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4683..5264)
                     /locus_tag="ACHA44_001733"
     CDS             complement(4683..5264)
                     /locus_tag="ACHA44_001733"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFM8905005.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5380..5477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5836..7128)
                     /locus_tag="ACHA44_001734"
     CDS             complement(5836..7128)
                     /locus_tag="ACHA44_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8905006.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7125..7748)
                     /locus_tag="ACHA44_001735"
     CDS             complement(7125..7748)
                     /locus_tag="ACHA44_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905007.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7751..8587)
                     /locus_tag="ACHA44_001736"
     CDS             complement(7751..8587)
                     /locus_tag="ACHA44_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905008.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8604..9491)
                     /locus_tag="ACHA44_001737"
     CDS             complement(8604..9491)
                     /locus_tag="ACHA44_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905009.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9507..10790)
                     /locus_tag="ACHA44_001738"
     CDS             complement(9507..10790)
                     /locus_tag="ACHA44_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905010.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11473..12156)
                     /locus_tag="ACHA44_001739"
     CDS             complement(11473..12156)
                     /locus_tag="ACHA44_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFM8905011.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12174..12791)
                     /locus_tag="ACHA44_001740"
     CDS             complement(12174..12791)
                     /locus_tag="ACHA44_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HFM8905012.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12801..13625)
                     /locus_tag="ACHA44_001741"
     CDS             complement(12801..13625)
                     /locus_tag="ACHA44_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFM8905013.1"
                     /translation="MNKFLGFRAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13618..14178)
                     /locus_tag="ACHA44_001742"
     CDS             complement(13618..14178)
                     /locus_tag="ACHA44_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905014.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14352..15308
                     /locus_tag="ACHA44_001743"
     CDS             14352..15308
                     /locus_tag="ACHA44_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8905015.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15613..16569)
                     /locus_tag="ACHA44_001744"
     CDS             complement(15613..16569)
                     /locus_tag="ACHA44_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HFM8905016.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16673..17275
                     /locus_tag="ACHA44_001745"
     CDS             16673..17275
                     /locus_tag="ACHA44_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8905017.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17363..18862)
                     /locus_tag="ACHA44_001746"
     CDS             complement(17363..18862)
                     /locus_tag="ACHA44_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFM8905018.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18878..19402)
                     /locus_tag="ACHA44_001747"
     CDS             complement(18878..19402)
                     /locus_tag="ACHA44_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFM8905019.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19700..20449
                     /locus_tag="ACHA44_001748"
     CDS             19700..20449
                     /locus_tag="ACHA44_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8905020.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20440..21225
                     /locus_tag="ACHA44_001749"
     CDS             20440..21225
                     /locus_tag="ACHA44_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8905021.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21194..21910
                     /locus_tag="ACHA44_001750"
     CDS             21194..21910
                     /locus_tag="ACHA44_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8905022.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
BASE COUNT         6460 a         4736 c         3837 g         6902 t
ORIGIN      
        1 ttttgctatt ctattttaat tgaattttgc aactcactct tctttattcc aaacctactc
       61 gtttgaatat cacgtcaacg tttttcaaat gatgatgata atcaaaggca tcgtctattt
      121 cttctttaga taataccgat gtgatggttg gatcttcttc taacagcggg cggaactgca
      181 cttggttgtc ccaagcgtac gcagtttttg gctgaacaag atcataagct tcttctcttg
      241 tcattccctt atcgatcaat ttcagcataa cgcgctggct ataaatcaaa ccaaatgttg
      301 cagacatatt ccgcttcatg ttttcaggaa agaccgttag atttttaacg atattgccaa
      361 aacgatttaa catgtaattc aataaaatcg tggtatcagg caagatgatt cgttctgctg
      421 aagaatgaga aatatctctt tcatgccata atgtgacatt ttcataggca gtgaccatgt
      481 gtccgcgcac cactctggct agaccagcca tattttctga accgatcgga ttccttttgt
      541 gcggcatagc agaagaacct ttttgacctt tcgcaaaaaa ttcttctact tcacgtgttt
      601 cggatttttg caatccgcgt atctctgttg caaaacgttc aatacttgta gcaataagcg
      661 ccatcgctga gaaatattca gcatgtagat cccgagggag aacttgagta gagatttctt
      721 gtggacggat ccccaattgt ttacatacat actcttcaat aaatggcggg atatttgcga
      781 acgttcccac tgctcctgag atttttcctg cttctactcc ttttgccgca tgttcaaatc
      841 gttcgatgtt ccgtttcatt tctgaatacc atgtggcaag tttcaatcca aaagtagttg
      901 gttcagcatg gacaccgtgt gtccgaccca tcatcactgt atatttgtgt tcttttgctt
      961 tttcgccaat gatttccgta aaacgtttca agtcttcccg taaaatatca tttgcttgct
     1021 tgatgagata gccataagca gtatctacga catccgtact tgttagacca taatggaccc
     1081 attttctttc ttctcccaat gtttcagaaa ccgctcgtgt aaacgcaacg acatcatggc
     1141 gtgttaattt ttcgatttcc aagatccgat cgatatcaaa tgatgcatgc tcacgaattt
     1201 ttttcacatc ttcttgcggg atttcaccaa gttctgccca agcttcatct gctaaaatct
     1261 cgacttcgag ccacgcacgg tatttgttct cttccgtcca gatattcccc atttcagggc
     1321 gtgtataacg ttctaacatg ttttttctcc tctaatccca aatattcgtt tgtttgattt
     1381 cttccagcgt ttgctggatg tcttctgtca agatagtgat atgtcccatc ttgcgacctt
     1441 tttttgcttg tttttttcca tagaaatgga attgccagtc tggcttattc tcaatcaagc
     1501 gatagctttc cagcagttgt tctcccaaaa cattgaccat tactgcattg cttatcaaac
     1561 gtactttttc tcctaatggc caaccacaaa tacctcgaat atgggcatcg aactgactca
     1621 tagaacaagc ttcaatagaa taatgtccag aattgtgagg acgaggagct aattcattca
     1681 catatagccc gcctgttttt gttaaaaaca tttctacccc taaaacacct gaaaggctca
     1741 ctgcttctgc aatcacacgg gcaatacgct cagcctcctc gatcacatcc tgatcaatcg
     1801 cggctggagc aatcgtttca tgaagaatat tgttgtggtg gatgttttcc acgaccggaa
     1861 aagtcgtgaa atctccttga ccatttcctg ccaccatgat cgaaatctct ttttcaaacg
     1921 ggatccatgc ttctaaaaca caggttcctt ctcttaaaag gtccatggca ggagctagat
     1981 ccgcccgact tttcaaaaca tactgcccct tgccatcata gccgcctcga gtggttttca
     2041 aaacacaagg atagccgatc ccgtcaatcg catcttggat atccgtcgga ctgacgatcg
     2101 ttgcataagg agcaatcacg atgttattcg tttccaaaaa agatttttct aacaggcggt
     2161 cttgcgtaat cgcaagcaag tctgtccctt gaggaataaa tgacatgggt aaaatcgcat
     2221 ttaacgcctc tacactgacg ttttcgaatt cataggtaat gacatctgtt cttctggcaa
     2281 gctctttcag cgcaaaggtg tcatcataga cactttcaat gtgccaatct gcaacttgtg
     2341 cggctggaca atcgctcaca ggatccagca cacctacttt aaagcccatt tcttttgcac
     2401 taatcgccat catacgacct aattgaccgc caccgacgat cccgatcgtt gaaccaggca
     2461 acaaaggttt aaccaagttg atcactactt tctataacag ttttagccaa cattgaacgt
     2521 ctttcagcca atttagcctc caattctaaa tcatacatgg aaagcatctg tatcgcaagt
     2581 agtccagcat taattgctcc cgcttttccg attgctgtag tagctacagg tactcctcca
     2641 ggcatttgga caatcgaaag aagcgaatcc aaaccattta atgttcgtga ttggaccggt
     2701 acaccgataa ccggcaaagt tgtttttgat gcgaccatac ccggcagatg cgcggctccc
     2761 cctgctcctg caataatgat tttgattcct ttttttcgag cgttttgtgc atattcaaac
     2821 atcaaatctg gggttcgatg agcagaaacg accttttttt cataagacac accgaattca
     2881 tccaatattt gacatgcttc tttcattgtt tcccaatctg aagtactccc cataatcaca
     2941 gagatttttg ctgtcattgt ttttcctccc agtttttcta cattcatatt ttaacaatca
     3001 ctttttttga tgtcaaagaa atttcgaatg ttaacaaaga aaaacatttt attgttcgtg
     3061 tttattaaag ataaaaaaaa ggaatgtgac aaaagttagg tcacattcct ttttatggat
     3121 aaacggtgtt ctcctgccaa ctcctcggtc acaaggaaca atattcaaca aacatgagaa
     3181 gctttttttt gaaattgttc cttgtgactc ggagatgaac ggcaggctca cgacctcttc
     3241 cttcaacggt gttcaatcag ctgaccctcg gtattagctc aaacaaacgg caaaatacga
     3301 ggaactattt ttccgtttgt ttctctaaca atcaggtcaa aacgctgatt tcacaacctt
     3361 tcttttgctc tattacttct tgaaggcttc ttccaatatt tcttcatctt ttttcaatcc
     3421 attaaagatc aaattcaata tgatagccgt aatgctactc attacgatgc catttccagt
     3481 gaacatttgc aatgtttgcg gcatttgact gaataatgtc ggcgtaacat tgaaacccag
     3541 cccaaatcca acagaaacag caacaatcaa tagattttta tcattgctga aatcaacttc
     3601 caacagcata cggattcctt ggactgccac catcccgaac atgactaaca ttcctccacc
     3661 aagtaccggc tcagggatga tttgtgctaa cgcaccgatt ttagggaata atcctaaaac
     3721 aatcaagaag aatgcagaga aatagatggg ttttcttgtt ttgattcccg aaagttgaac
     3781 caatccgaca ttttgagaaa agccagtata agggaatgta ttgaaaatac cgcctaagat
     3841 aacggcaagt ccttctgcac gatatccctt tttcaattct tcttctccaa catttttccc
     3901 agtaatgtca ccaagtgcaa aatatacacc tgttgattct accatgctga ccatggaaat
     3961 gatgatcatt agtaaaatcg accatacgtc gaaagttggt ttaccaaaat aaaagggttg
     4021 tggaacatgg aacaacggcg catctccaat cgcgctcaga tcaatcattc ccatgaaagc
     4081 agctaagatg cttcctccga ctaaaccaat caataccgcg attgaacgaa tgaacccttt
     4141 tgccagcatt tgcacgccta caattaggag aatcgtcaca aatgcaagta gcagatttgt
     4201 tttgtcacca aaatcagctg ctgttgcatt acctccaccc attttctcga ttgctacagg
     4261 aatcaaagtc aaaccaataa cggtgataac cgttcctgtt actataggag ggaatagttt
     4321 tttaatttta gaaaagacac cagaaactaa cacaacaaag atcccagaag cgataataga
     4381 tccgtagata gctcctactc cttcattggt tccgatcaag atcagtggag ctactgcttg
     4441 gatcgcacat cctaaaacga caggcaagcc aattccgaaa aatcggttaa ctgtcaattg
     4501 caataatgtc gctaatccac acataaagat atcaattgaa atcaaataag tcatctgttc
     4561 ttgattaaag cctagtcctg taccgatcaa aagaggcacc gctactgctc ctgcatacat
     4621 cgctagcaga tgctgcagcc ctaaaaccgc agcttttcca ttttgtgttt ctttttccac
     4681 gattatgcgt cctcctcaag aaactcaact tgtccattcg tcaaggaagc aatacgcgca
     4741 agagaaacga ctctcaatcc catttcttca agtaactgac gtccatcttg gaaagacttt
     4801 tcgatgacga tcccgattcc ttctacttgc gctccagctt gctggcataa ttcgatcaat
     4861 ccttttgccg cttgtccgtt tgccaagaaa tcgtcgataa tcaatacttt gtcttctgaa
     4921 gataaaaatt tacgagaaat agaaatcgta cttgtcactt gtttcgtaaa agaataaaca
     4981 gaagctgtca ataattcttc atccatcgtc aaactttttg cttttctagc gaagatcatt
     5041 ggaacatcta attctcttgc agcaaataaa gctggtgcga tcccagaagc ttcaatggtc
     5101 acaactttcg tgattccttg ttctttaaat acttctgcaa accgttttcc catttgttcc
     5161 atcaatacag ggtctacttg gtgagtgacg aagctgtcga cttttaatac gccctcgcct
     5221 aatatacgtc cgtctttttg aatacgttca actaattctt tcactttatt tgctccttta
     5281 tcatttatta ataccgaaat aaccgctttt gtgcaaaaac ctactatgtt tctgttaaag
     5341 aaaaacaccc tccactttat cataagaaaa gaagggtgaa atagccaatc ttttacttat
     5401 agtccagcat ttacggtact ggctagaaac tgtcatccat attatgacga tatataagta
     5461 ggtatgtaaa actaataata gtgtaaattc attttcttgc aactgttttg cacgtgaagg
     5521 cagtttgcca tatttgaata aaaaatgttg tttcaaatat tgaaaacagt ctaacactac
     5581 tttctgaaac tgacaacgtg gtttacttat ttttttgtaa aatctaatga tttttaatgc
     5641 attattcctt tttgttcggg tttaaatagt tatttttatt ttctcctatc taaaaaagca
     5701 gcccacataa aaagaaggca tgacaaaaag catgtcatag cccctttttc cgaataaata
     5761 gtgttcaaaa gacagcctat cggcaataag gcaagatttt ataaactacg ggaagtaatt
     5821 tccgtaaatc tattcttatt cttttaagct gacaagctcg tcaatcacta ctgcttttcc
     5881 tgaagcaata gaatgatttg ctgcgattcc tgtcaaaatc gacaaaatac catctacgtg
     5941 agttgctgcc cgattccacg gatcttcttc tttttcttca ccaaatagat catctaataa
     6001 acgcggatct cctccgccat gtccgcccgc ttcttgttcg acgattacct catatggctc
     6061 accgaacatc ggatagacat ggatggactg aagaacggct gctccttcat cctttttatc
     6121 tccacctgca ttgatgtaag acttttcgat cactttcatt tcgatccgtc ctttgctgcc
     6181 attgaaagaa acattgaatc cttcccacgg catatacgcg tttagcgaat acgtcaaaat
     6241 cgcaccagtt tcatatttga ccgttacgcc tagtgtatct tcaatagaga tatcatcgga
     6301 aaatacactg cggtcacgaa tatatccact gtctttttct gcttctaaat acatttctcg
     6361 caattcctca ttggctgaca ggtctatagc aaatggatca gctttagcta gttcgcttcc
     6421 atagcatcga ctgtaaaata tttctactcc gcgtttctcc gcattatgca tcccataaaa
     6481 gtttaatcca ccgaatgcaa ataccttttg gggacggctg ttcaaccaaa aattcactag
     6541 atcaaaatga tgggtagatt tatgaacaag aagcccgccg ctattgtgct tatttcgatg
     6601 ccatcttctg aaataatctg ccccgtgttg cgtatttaac agccattcga agtggataga
     6661 aaacacatca ccaatcacat cactttctat cagttccttt atcttcgtgt gatacggtgc
     6721 ataacgataa ttaaaagtga ctcgcaggct tcttcccgtc tgtttttgga ccgttattat
     6781 ttcttgagct ttttcctcat cgatagtcag aggtttttcg caaataacat catatccttt
     6841 tttcattgca cgaatgatat aatcatgatg cgttcgatct actgacgtaa taatgacttc
     6901 agccggccgt acgtcatcca gtagttgctc aaattgtgat ggcatacatg tctttacttt
     6961 tttctgtcca tacttgttca caagtatccg atttgcataa tccatccttg tttgagatac
     7021 atcacaaaaa ccgactagtt cacaagtttg atgatacgta gaggcaattg cctcataaaa
     7081 aaaacgcgct ctcccaccaa gtccgacttg tacatatttt ttcttcattt aaagtcattc
     7141 tccttttctt ttaagcaaaa tactgtgaca aatggcagcc ctggatacag taatgcagtg
     7201 agtaaaaaat catgctgtat acaccagaac agtccataca aaaggcataa ataccacaga
     7261 aaccccttga cgttttttga caacagccag atattccttt tgatcaaata aacaagacta
     7321 tctgctttat cttctttgca gatatcaatt agcgtactaa aaaaactatg ggatacaaaa
     7381 agcaacagac acaatagaaa aggaatcaga taccaagctc caatctttcc cactaataaa
     7441 taccaatcaa ctgcaaaaat aatgacaaac agctgaaaca acgtaatgat gaaacacatc
     7501 cgtttgattt ttcctttcct ccaacatacc tttttttctg ccaagtaatg gacagataga
     7561 taataagaag gatacatcat caaaaaataa atcagataga caacacgagc agacagcact
     7621 aatcctaaag tgaacaaaca tcctagaact aaagcgaaaa gagcattact gataagactg
     7681 gttaaaaaaa tccgacagac atagtagttg agcttgtaaa gaaacatctc ttctattggc
     7741 tttttcatat tcagcccttc agaccgcttg tactgatccc ctggacaaga tatttattga
     7801 agaataagaa aatcagaaaa accggtagca gcgacaacgt agacatcgca aacatactcg
     7861 cgtaagtcgt actagatcca gcgtctgcaa ataatttgat ggcaatcgat accgtgtaag
     7921 atctcggctt gtttaaataa atcaatggtc ccatgaagtc gtcccatttc caataaaact
     7981 ggatgatgat cgtcgtgatg atcgaaggtt tcagcaaagg aaaaataatt ttggtaaaga
     8041 cactatactt gctgcatccg tcaataatcg ctgattcatc taactctttg gggatgctga
     8101 ccatgaattg catgatctgg taaatgaaaa atgcttgtcc aaaaaaatgt ggtaaaatca
     8161 gcggcacata cgtcccaacc aaattcagcc gattgtaaat aataaactgc ggaatcaaaa
     8221 tcacttgtgc aggaatcatc atcgttacta acatgacgac gaaccagaat ctttttcctt
     8281 tgaaatcgat tctcgataat gcgtaagcaa tacaagtcga gctcagaact gtccctatcg
     8341 tcgaaactgc agagatgaca aacgaattcc taaagaaagt aaaaaatgaa attccagcaa
     8401 accccgccca tccttttgcg taattttccc attgaaaagt ctctggaatc aaattcagcg
     8461 aaccactcat aatctcttga tccgttttaa aagaacctgc gatcatccat aagaccggat
     8521 aaatcatgac tagacctaat agcaaaataa aaccgtattg aaaccatttc gttactgtat
     8581 tattcatttc ttcctcctct cattcacaca acgacccatt tttttgatga tttgaaaatc
     8641 agaaaggtga cagctcccat aatcaacagc atgatccacg aaatagcact agcatacccc
     8701 atagaattaa aagaaaaagc ttggttatac acataaagag cataaaagtt cgtggcatca
     8761 ttcggaccac cttgggtaat gataaatgcc tgcgtaaaag tcataaacgc cgcgattgtc
     8821 tgcataacca agttatacaa aatgatcggc gaaagaagag gtaacgtgat cttgtaaaac
     8881 gtctgccatt tgttcgctcc atcgatctct gccgcttcat aatacgagcc tgggatctct
     8941 ttgatacctg cagcaaagat gatcattgcc gaaccgaatt gccaagcaga catcaaaaca
     9001 agagggatca tgaccagttt ttgatcgcta aaccaggaaa gcgcaggtgc gcctaatcgt
     9061 tccaggaaaa tattcactaa cccgttttta gaaaacaact cccgccaaac tagagcgata
     9121 gaaatactgc caccgatcaa actaggcaca taatacaaag aacgaaagaa agtcgttcct
     9181 ttgatattcc tcgttaaaag ataagcaatc aatagaccaa agatcagttt cacaggtaca
     9241 gatgcaaata catagagaaa agtgactcgg atggagttca aaaaacgggc atcactcagc
     9301 atctttatat aattatccaa gccgatccat gagactggat ttcccaaacg ataatcggtg
     9361 aacgaataat aaagtgacaa gatcatcggt atcactgtta tcaatagaaa tcctaggatg
     9421 aatggagaag caaatacgta accgaccaca ttgtcttgat ccaagaaccg ttttagtttt
     9481 tcttttttca tgccttcctc ctgctgctat cctagaatgt ttgacgcaaa ctcataaaga
     9541 ctctctgctg cttcttctgc tgttacttct tcataaatga ctttttcaac taaaagattg
     9601 tattggtctt gaatttccgg attcaaagca ctatcaaaga cattcacttc gccgttgtcc
     9661 atatcggaaa tcatttctaa ataatcaaat gtcgcttgca aagttgcatc atctgcacga
     9721 gcgagctgtt ctcgaatatt cgacatgatt ggtacaccac gttctccttt taaaatttca
     9781 ttggcttctt cactgttttg aaagaaatca ataaacttag cagcttcttc tttatgggct
     9841 gagctttttg ccatactcag catttgcgaa gaacgtaaag ggataccaga ttctccatct
     9901 gcttttctgc gaggtggatt aatcaactga atctccatat cttcaggagc ccctgacaat
     9961 atttctgtca actggttact tgatagaaac accattgccg cttctctatc cctaaccgcc
    10021 tgaaaagaca gatccgtcgt acttgcagct ccaccacctg gttctggatg ggcccctttg
    10081 tctaccagat ttttacgcag ctggaaaaaa tctgttagat agtctggatt ttcaaatccc
    10141 aagctgttag atagatcttc ccgattataa aaattcaatc ctttctcaac ttgtggtatt
    10201 tgaataatac agccagctat aaaatcttcg aatcttgaaa tagcatagat tcctaattct
    10261 tcagtgattt gatagctgat ttgttcaaat tcatcccatg tccaatttga agctggttca
    10321 gggatattta attgttcaaa tatattttta ttgtaagcaa tcgcaaacga attcaccccg
    10381 ttagacagtc caactaattg cccgtcgtaa gtcgtcgttg ctaaatagga ttcatctgca
    10441 ttagaaatat caattatccc ttgctccaca tattcatcta atggttcaat ctgattgatg
    10501 tattgaggaa attgattgtt cagttggaaa atgtcccaga tatcatctga tgctgctagt
    10561 gtacctaatt ttgtgtaata tccttcataa tcataaaatt cataatcgat cgtcacatgg
    10621 ggattttttt cttcataaag ctcgattact tttactgttg catcgtgtcg gacttgcgaa
    10681 ccccaccaag ccatgttcag cgtgattttt ttgtcatctt cttgcccaca agccgtcaag
    10741 aagaataaca taacacttaa agctaagatg attctcattc ttttcttcat tctctgcacc
    10801 tctacttcca ttgttatttt tcaatatact gtatttttat acagaacata tttattaaac
    10861 gatctttttc aaggggaata aaaaccaaga cttgccttat cgttctgcct aggatgaaca
    10921 actcaatact ttcaagaaaa attagatagt gctgattctt ctttcatcgc taaaaacact
    10981 tcttgcttca atgatgggag gataattacg caaagaaaaa aggatatcag acaactgccc
    11041 aatatccttg acatagctaa ataagcatgc ttcttcgtgt gcgctcttcc ctaaaaaaca
    11101 gaaaaataac tagtaattct gtatgaaatt aggtacaata tacacgtcga tgcacaagca
    11161 ttgactatgg caacttgatt ggtcagttcc cgctgacttt tcaagccccg acaatttagc
    11221 tgctactaaa ttgtcgggtg ccttttttag tttttattcg tttttcattc tatagaaagc
    11281 gcttcaataa atcaagacat ttttttgtta gaatgctaat aataacaaaa ggttttaact
    11341 cttcttatgt acattacagg taaaaaaaca aaaaaatttc tctttttttt gaattctgaa
    11401 caaatagtac tggagtacaa aagacgagga agagccacta tctgctcttc ctcgtctttt
    11461 gattctcacc tgttattcat gattcttttt ctcagtttcc cgatattttt ccattttacg
    11521 actaaaaagc tctccgctac ttggtggcgt atatgtgatg gcatttgctc ctgcttgaat
    11581 ggtctcagca atactttctt ccgttggacc acctgtcgca atgataggaa gttcaggata
    11641 ttttttacga aaatggcgta ctgcttctgc agtttcttta cctgcactga tattgataat
    11701 cgaaatacca gctgctaact tttcatctat ttctgtatat ttcgacgtta ccgtactgat
    11761 aactggaata tcaactactc gacaaacttc ttttactgtc tctaaaggcg taggaccgtt
    11821 caaaacgacc cctaacgaac cttgcgcttc tgcgaacatg ctcatgtagg aagaacgagc
    11881 accttgtgtc aaaccaccac ctacaccgga gaaaactgga atgtcagctg cctcaataat
    11941 gcttttagta atcgctggat gaggtgtaaa aggatacaca gcaattactg cattcgcatt
    12001 tgtattacgt atgatcgcaa tatccgtagt aaagatgatc gaacggatca attttccaaa
    12061 tatcttgatc ccgcttgctt ctaaaatgat ttccggtact cttacgatat cttttctcaa
    12121 ttccgtcatg atttctggcg taaattgttc tgccatcagc cacttctcct ttgctatctc
    12181 cggtcatact gattcaattg atacgtctcg atgacctgga tgattttttg cgcataaccg
    12241 gggtctgtcg cataacctgc atcttgcaat gcttgtgcgg cttcctgata gttggtggcc
    12301 gtaatcactt tttcatacag cgcaggattc caatctactc cttggacgaa cagttgtgta
    12361 tggtcatcca tggactgttc ccatgaatca tacactttga agtctccttg gatagttatc
    12421 cattcttcgt taacaaattc ctttgtttcc aggcttactt tcttctgatc cccataagcc
    12481 ttgatcccga aaagattatt gtatttgcta gccaaggtac tttttcccca atttgattct
    12541 aatatagctt gtcctaaaat gatactagga agcactccat aaccatcttg tagttcttgg
    12601 gcatgcggaa caagccgttc aataaattgc tgatgggatt gcgcttcatt ttcttgctga
    12661 ttatcagtaa aaggagaaga cagattcttc aaggaaaaaa caaaggctaa gagtatcact
    12721 gctacaccgg caaccactgc tggccaacgg atattcttgc gcttttgctt tttccacttt
    12781 tttttcgcca tgatcaattc ctacttttca tttaattcat tcagatattc ttctagcgtc
    12841 aattggtgtt tggtgatata ctcagcgttt tctttaccga cataacgtaa gtgccaaggt
    12901 tcatatgtga tcttagttat atcttcgtgt ccttttggat aacgaacgat aaatccatac
    12961 tgcgcagcat tatctgcgat ccatttagct ccaggctgat ctccgtaact tgcatccaat
    13021 acagtagaag gataactgtt gtaccagttt tgatcgacga catcgactgc cagacctgtg
    13081 tggtgttcgc tgtatcctgg ctccgtaatc gtttctttag taatctttgt cgcctcctct
    13141 tctgatacac cttgacgact catcacatct tggacatttt gttcgaaaac ttgttgttgg
    13201 gatgatacag accggtaagc tgagaccatc actaatggaa atccagctgc ttcagcagcc
    13261 ttggcaaatg cttcatattc tggagcaatg cgtttgtcga tcaagtatcc gttacttaat
    13321 gatgccaatt gagtagactc atcaatttct ttttctaatt tgtgatctgg accaaccaaa
    13381 actagcttcc aatcgtctgt tttaacatct ggcaattgtt tggacgtacg atcttttgtt
    13441 tctttgtcct ttaccttgct gctggttgtt tgactgttct tttctttact agcagttgtt
    13501 ttggtgcttg ttcgttcgac tgcttgattg ccggcttgaa catgcgaatc atcatcgcta
    13561 aaaataacat agcccattgc tgcgatcata atcacaactg ctctaaatcc taaaaattta
    13621 ttcacttgaa aaatcctttc gttgttcttc acgttcccca ttcgcctcat gaacgtaagt
    13681 ttcttccaga ttttcattca cccaatcgag aagttcgatt ttttctcctt cgatctgttt
    13741 ttgaacatct gctggcagac gaaaattcaa aatatcatca tagccccatt catcatattg
    13801 aatacttact ttcaagtcaa ggtatctacc agcctgctga ttatttttca aagggatcag
    13861 ctcgactttt gctgctttgc tgctaagcca tttttgcgcg atgcgtgttt tgatttgctt
    13921 gtactctgca acggcaacgt ttcgtttttc gggaaaaata atcgtttgac gcaaaatctt
    13981 ttgcgttagc atccattcat aatcagtgaa aagatttttt actttctgca tcgcttcaga
    14041 tggcagattt ttctcgccat ctttccattt ttgccattgt tctggactga tcagcaagta
    14101 ttcatctaaa aaattttttt cagtttcaaa ctgttctaat aatacactca taataagatc
    14161 aatatatact tgatgcacgt ttatcccctc cttttaacta ttctacttaa gttttgatca
    14221 aaaagcacct cttaaatcgc tgctttcatt ttttttaatg tttcaagtcc tctcctcttt
    14281 attcttcgtg atttttgtta taataataga cagacaagaa actcaatcca actatgaagg
    14341 agcgttttca gatgaaaaaa tatcaatggg gaatcgttgg tcttggtacc atcgcacatg
    14401 agtttgccga aagcttcaat caagagacga gcgaactaac agcagttgct tccagaactt
    14461 cagaaaaagc ggaaaacttc gctcaccgtt acaatattcc aaaagcttac ggaagctatc
    14521 aagaaatgtt agacgatgct gaaatcgaca tcgtgtacat tgctgtacca aatagacagc
    14581 atatcgacca tatcttggca gcattggaag caggcaaaca cgttctatgt gaaaaagcca
    14641 tcacgatgaa taaaaaagaa ctggctgacg ctatgcgctt agcagaagaa aagaatttga
    14701 tcttagcaga agcgatgaca atcttcaaca tgccacttta tcagcagtta cgctcaataa
    14761 tggatacagg aaaactgggg gctttaaaaa tgatccaagc acctttcggc agttacaaag
    14821 aaccagaccc taaaaaccgg ttcttcaatc cagaattagc tggaggagct ctgttggata
    14881 tcggtacgta tgctgtatct ttcgctcgtt tctttttgag ctctcaacct gaagtagttg
    14941 cctctacaat ggttcctttt gaaacaggcg tggacgaaca atccgtcact atcttgcgta
    15001 acaaagaaaa tgaacttgct gctgtcagct tgacttttca ggcaaaaatg ccaaaagtcg
    15061 gtatcgttgc ctttgagaac ggttatgtga cgattgctga ttacccgcgt gctgatcgtg
    15121 cggagatctt atttacagat ggtacaaagg aattcatcga aagcgggaat acatctgaag
    15181 caatgaatta cgaaataaag aatatggtac acatgattga aggtgacttg cccaatcgct
    15241 ctctcttctt gacaaaagat gtcatcgaaa tcttggatca aatgcaagtt ctatggaaaa
    15301 atatgtaatt ttaaaaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    15361 acaaaaatag ctcctcctat ttttcgtttg gctggactta ttaccgcaga ggaaagcttt
    15421 tgaacaccgt ttaagaaaga ggttgtgagc ctaccgttca gctccgagtc acaaggagca
    15481 atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg aggagttgac
    15541 aggagaacac cgtttaaaga agaggttgtg aaatcagctt tttgacctga atattagctg
    15601 aattcacaac ctctattttt ggattttttg ttgggagaat tctgccttga tttcaataaa
    15661 acttttaaaa gactgcaaaa actggaacaa ttctgctcga tcctcgaatt cagggcgact
    15721 agtcggcaga ggcatttgcc gatacaatac ataaacttct tcaatacgtg ttaaaatttg
    15781 tctgccatca tttgactctg aaaatgtttc tgcagtataa atcaataaac catagatatg
    15841 ttttccataa taagctggtt gctggattct ctctagattt tccagcatgt ctttaatcac
    15901 attggcctgt gctcttctca tagagaagta agtttcgtag tatagcggct gtctgagcca
    15961 gtagttttct tgatgttctc tggcatcttc ttggctttcg cgaatgaatg tcagtaaatg
    16021 ttcgcatttg atctgcacat cttctttatt ttctgataaa agaaattctg ccatttgtct
    16081 caaaatattt cgaaattctt tttcgatctg tatttggttt tccttcagct gcttcgtacg
    16141 atctggcatg taaacatttg caagcaaagc aaatactaat ccaatcgcca ttaatcccgc
    16201 ttcattgccg atcagctgcc aagacatgtt ttcctccact aaataatgag tgaccaaaac
    16261 cgaatttact actatccctt cggttaattt gaagcgagag gcaagcggga tgaacaggaa
    16321 taaaaaaaga ctgaagccaa aaatagtata cccaacagca aaaaaactga acagtgctgt
    16381 tgccgtagct aatataaagg ctgcgattcg attcatgccc gtacgcaatg tagagcgctt
    16441 ggtatttcct acacttaata atgcaatgat ccctgcagct ggccaataca acagcgataa
    16501 gctacccgct gtgaccatcg aaaagaagac actgatcact gttttgactg ttcgcattcc
    16561 tatacgcatt tacttccccc ttttttattt taattatact tgaataatga aaaactagct
    16621 atcagacaaa agtttaggta tgatagtaaa aagaccaaaa aggagaatct ctatgcaatt
    16681 tggccgtttt cgactaggta tgcgtacgat caaaagtgcc ttagctgttt ttttatgtat
    16741 cttgtttttt catttgactc aacgaggaat cccgatgatt gctgcattat ctgctgtttt
    16801 ttctttgcgt caggacttga ctacaaccgt ttcttttgga aagtctagga tcatcggcaa
    16861 tacattaggt ggtacattag ctatcattta tttttttgtc aaaagctatt ttcacaatga
    16921 ttttttggta gaattgatct tgcttcctat tctagttatt attgtgatcg tcgtgtcaga
    16981 tggtattaac aataattccg gtatcatttc cgcaatagct acgttgttgc tgattgcgtt
    17041 gagcatccct caaggcgaat ccgctttgta tgctatccaa cgtgtactag atacttttat
    17101 agggacattc attgcaatcg gtatcaattt ctttcttcgt cctcctgaaa cagaaaagaa
    17161 ggaagaacta tcagaagatt tggtcgaact ccaaaagaaa gaacgattgc taagaacaaa
    17221 tttacaagag atagaagcta agatccaaaa acaaaagaaa aatagcgagg actaattttc
    17281 ttttgcaata aaataaaaaa atggagagaa gaactttaaa aagtcttcct ccatttttga
    17341 ttgaacggtg aagtgtacta ctttattctt ccttcatcga gttttgttct gcctcttttg
    17401 gcgcttcata catatttact tggctagtag tcccacgtgt cgacaaattc tctacgattt
    17461 ccttcaagtc aagtcctgtt gtcgctttta gtgtttcttg agtagaagaa agcaaatttg
    17521 tggcataatt tgtcactcgg ttcgctccac tggtttctcc tgaactgctt cctgtatcga
    17581 caacagagat tttttcgata tttcctagcg gctgtgctgc ttcgcgcatc aattgcggta
    17641 gcatatcgat aaccatactc aagacagctg cttcgccata ttccttgaag gcattggcga
    17701 tcttttgttt tgcttcggct tctgcttttc cttttgctag tgctgcttcg gcttctgctt
    17761 gtccagctaa acgtgtctta ttggcttccg cttctgccag tgcttccaca cggaatcgtt
    17821 ctgcttcggc ttcagtcact tctttgactt tttgagcttg tgcttcttgt tctttagcgt
    17881 accgatcggc atctgctttt ttcttcactt ccgaatcata ttgcttttca cgacgtgtga
    17941 tttctttttc ttccagttcg atttgttttt gacgttctac gattttaatc tgcatttcct
    18001 gttcgattac ttgttgttgt gctcgggcac tttctaaatt gtaggcttgg tctgcttttg
    18061 ctttagcaac atcttgttct tgtttgtagg ttgctaattt cagttctttt tcttttaatg
    18121 attctgcgat ctctgtttgt cgctgtaatt cagctgcttg tgattctttt tctgcttgag
    18181 cttttttgat acgcgtttct tttagagctt ccgcttctgc tatatctgca tctcttttga
    18241 cttgggcgat tcttggtttc cctaaagagt caagataacc gtttttgtct ctgacctctt
    18301 tgatagtgaa agaaacgatg atcaacccca tttttgcaag gtctacagaa gcgacttctt
    18361 gtacgctttg actgaactta tcgcgatttt gatagatttc ttctacagtc atagaaccaa
    18421 gaatcgagcg caagtgtcct tctaatactt ctcttgcttc attttccagt tcttctcttg
    18481 tcttgcctaa aaactgttct gctgctgtag caatctcttc tacagatgaa ccgattttga
    18541 tgatcgaagt accatcacac atcaccggta cgccttgttc cgtatacact tcaggggtgg
    18601 agacgtccaa tttactcgat aatagactga tccgattcga acgttgaaaa actggcaaga
    18661 cgaatgcgcc acctccacgc acaattttga ttttgttatt gctttcatca atgtgcacat
    18721 ttttgcttcc taaataactt ccactgatga tcaacgcttc atccggtttt gctgtctggt
    18781 acttcgctac aaagacgatc agcaacatca gtaaaatcaa agctgcgatt gcgatcgcga
    18841 taaaaacaac tggtatttcc atttaacaca cgctccttta agcttttatc atgctttcat
    18901 aaggcatgac ataacagatt ctttccttga tctcaatgat caatactttc gttccccgct
    18961 cagctgtctg ttcttggggg ctgtaaaaac aagctggacg actaatggaa ccggtaacac
    19021 ttttcaactg aatttctccc atacctcgta cagggatcgg tgtgatcaca acagctactt
    19081 gtccttccag agttttttca gaagacgaca tcgttgattc ggcatgtttc atcggcatca
    19141 atatatagaa attaagcaaa aataccaaca ctatcgatat gcctccacta gcaataaata
    19201 aaaataaaga tgattgatct gttaaacgct cacctagaaa accaaataag cttgtgaaag
    19261 ttaaccaagg gataataagc atcggatcaa gcgggccatc aaaatcgaat acatctccaa
    19321 aaataatcaa taggactgcc aatcctgcac aaatcagcaa tgtataccaa taaatcgtct
    19381 caagtgcgat tccccctatc atatacacgc ctccttccat ttctacgtct atcataccaa
    19441 tgtttgaaaa ttctgtatag cttcttttac cgactcagtg gtgtttttta taggaaggtt
    19501 gtgtttatcg ttgacaattt gttaaatttt tgttttcatg ttaaaatttt cacaataaat
    19561 tctttcgagg tagcctatgt ttcctttttt taagaaaaaa ccattaaagc tccagaacac
    19621 gatcatcctt gtcgtttgtt tctccacatt cgtttctttg attgtttctg ccatattgat
    19681 caggaatttt attctttcga tgaaccgatc tagtgtgtca gataaaatct ctagcattgc
    19741 taaaatcgtt gccaatgata aactcatcca aaaaaagctg cagactggtg acccgacaca
    19801 tgcgattcaa ggaataacaa atgaaatcat gattgcatcg aatttagatt ttatcgttgt
    19861 catggaccct gaaggcattc gatattctca cgcagaccca agttacatcg gtcttccttt
    19921 ctcaagtcat gaagatgtga aaaaagtgat tggaggacaa gaatttttct ctgaaaaaga
    19981 aggaatgtta gggttcggct tccgctattt cgtccctatt tattatgatc aagagattat
    20041 tggtgcagtt tgtgttggct taacaaacaa cacgattgac aatcaagtca aagatggaca
    20101 aaaaatgatt gttttttcat tgattattgg acttagcgta ggcatcatag gcgccgtttt
    20161 tcttgcccaa aaaatgaaaa aagtactgct tggacttgaa ccacaagaaa tagcagaaca
    20221 gttaagtgaa aaaacgatta tcgaaaatga agtagatgaa ggaatcttag ccatctcttc
    20281 tgataaaact atcctcttga tcaatacacc tgcaaaaaac attttctacc aaacagagca
    20341 gctattggat ttcgaagtag gaaaaaaatc agtgataatc tatatgacgt tttgtttaaa
    20401 gagacgattg agcatcaaac gaccgttcgg actcaatcca tgtatttaaa tgggatagaa
    20461 actatttcta gcgttacacc tctttttagc aatgaccgtt ttttaggagc agtcgcaact
    20521 ttacgaaatc gatcagaagt aatgaaatta atggaggagc ttggggggac gaaacaatat
    20581 ataaactcca tgagagctca aacccatgaa ttcatgaata aacttcatgt catttctggt
    20641 ttgattgatt tgaagaagta tgaagaagtc acccatttta tccagcagct aaatcaaaat
    20701 tataaaaaag agattggcca agtctcaagt ctgatcaaag tacctgcaat tgccggtttt
    20761 ttattaggca aaataaacga agccaaagaa caatctgttg aaatgctgtt agaaagcgat
    20821 tcttttatcc ctaaaatgga gatggatgac tccatccatt cacttttgca agtgttaggg
    20881 aacttacttg ataatgcaaa agaagccgtg ctacaaaaac atgaaagaaa cggacaggtc
    20941 cggattcatt taaactatga cacagaagct gaagtattca tcgtgaccgt ctgcgatgac
    21001 ggtgtcggga tatcagaaga aatctcagaa aaaatattta cgctaggttt ttcgacgaag
    21061 ggaatcaatc gtgggtttgg attgaatctc atccattcaa tcgtcaaacg ccacggtgga
    21121 tttttagaat tttccaaaca aacaaatggc ggtacaatcg tctatctaga attaccaaaa
    21181 aaggaagatc aaaatgatga caaatgtcct gataatcgaa gatgatccaa tggttgcaga
    21241 attgaatcgt acttttgtgg aaagcatccc taatatgaac gtaatcgaaa gtttcagaaa
    21301 tgtagaagaa ggaaaaaaat atttcagaca aaaaaacgca gatctgctgc tgcttgatgt
    21361 atacatgcct ggcgaaagtg gtatccattt tttatcttgg cttagacagc aaggaaatga
    21421 tatcccagtg attttgatca ctgctgcaga tgatatcgaa accataaaga atgtgatccg
    21481 ctacggtgcc ttagattatt tgatcaaacc gttcactttg caacgttttc agctggcgat
    21541 cgaaaagtat cttgctttct ctcagacaat ccaaacgact gaaaaagccg atcagaaatt
    21601 gatcgatcaa gtattcaacc acaccactcc cattcacgaa ataccatcat tcatcgattt
    21661 gcctaaaggt ctgtcaaaac tgactttaaa aaaaataagt gcatttattc taaaagaaga
    21721 tactattttt tcaacaaaag aactggctca aaaaagctca ctgtcacgta tatccgtcaa
    21781 gaaatatttg aattttttag tagaacaaga tatattgaac gaaagaatca aatatttgga
    21841 aatcggccgg ccgctaacct gttatgaatt gacaaagaat gggcggaaaa agctcacaga
    21901 ttactgttga gtcatcgaat tatcccatac aaaat
//
LOCUS       DAYYGS010000051        21892 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000051 DAYYGS010000000
VERSION     DAYYGS010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.051"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(609..2105)
                     /gene="lysS"
                     /locus_tag="ACHA44_001751"
     CDS             complement(609..2105)
                     /gene="lysS"
                     /locus_tag="ACHA44_001751"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFM8905023.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="ACHA44_001752"
     CDS             complement(2216..3220)
                     /gene="dusB"
                     /locus_tag="ACHA44_001752"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFM8905024.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="ACHA44_001753"
     CDS             complement(3221..4108)
                     /gene="hslO"
                     /locus_tag="ACHA44_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFM8905025.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="ACHA44_001754"
     CDS             complement(4325..6436)
                     /gene="ftsH"
                     /locus_tag="ACHA44_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFM8905026.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="ACHA44_001755"
     CDS             complement(6526..7071)
                     /gene="hpt"
                     /locus_tag="ACHA44_001755"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFM8905027.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="ACHA44_001756"
     CDS             complement(7071..8516)
                     /gene="tilS"
                     /locus_tag="ACHA44_001756"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFM8905028.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8636..9106)
                     /locus_tag="ACHA44_001757"
     CDS             complement(8636..9106)
                     /locus_tag="ACHA44_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 domain-containing RNA-binding protein"
                     /protein_id="HFM8905029.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9152..9577)
                     /locus_tag="ACHA44_001758"
     CDS             complement(9152..9577)
                     /locus_tag="ACHA44_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HFM8905030.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9644..9913)
                     /locus_tag="ACHA44_001759"
     CDS             complement(9644..9913)
                     /locus_tag="ACHA44_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFM8905031.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9924..11519)
                     /locus_tag="ACHA44_001760"
     CDS             complement(9924..11519)
                     /locus_tag="ACHA44_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8905032.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="ACHA44_001761"
     CDS             complement(11534..15055)
                     /gene="mfd"
                     /locus_tag="ACHA44_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFM8905033.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(15072..15632)
                     /gene="pth"
                     /locus_tag="ACHA44_001762"
     CDS             complement(15072..15632)
                     /gene="pth"
                     /locus_tag="ACHA44_001762"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFM8905034.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15985..16959
                     /locus_tag="ACHA44_001763"
     CDS             15985..16959
                     /locus_tag="ACHA44_001763"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFM8905035.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17129..18529
                     /locus_tag="ACHA44_001764"
     CDS             17129..18529
                     /locus_tag="ACHA44_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HFM8905036.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18607..19329)
                     /locus_tag="ACHA44_001765"
     CDS             complement(18607..19329)
                     /locus_tag="ACHA44_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905037.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19408..19914)
                     /locus_tag="ACHA44_001766"
     CDS             complement(19408..19914)
                     /locus_tag="ACHA44_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8905038.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            20037..21836
                     /locus_tag="ACHA44_001767"
     CDS             20037..21836
                     /locus_tag="ACHA44_001767"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HFM8905039.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         6070 a         4769 c         3924 g         7129 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa gcgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcggca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc
      301 agttatctta tcatgtcgtt agtaacatgt caataacttt tttgttatgt ttttgaataa
      361 atattttctt ctgtcttttg gacaagtaat aatataccaa ctaaatttag cttggtcaac
      421 gaattttttg tattttttca agtttttttc aataaacgat tattcataac ttttctttct
      481 ttatcgttcg ttttcgtctt ctttaatgtc tagaatagtc ggcattagct tttgaacttt
      541 cgttattatt tattcctttc tacacagcaa aagctgccta aggaattcct tagacagctt
      601 ttattcgatt atctcattgt tgggaatagc aaaacatccc gaattgattg tgcatcagtt
      661 agcaacatta ctaatctgtc gatcccgatg cctagaccac cagttggcgg cataccgtat
      721 tctaaggctt ccaagaagtc ttcgtctaca ccgtgagctt cgtcatttcc tagttcacgt
      781 tctttttctt gcgcttcgaa tctttctctt tgatcaattg gatcgttcag ttctgtaaag
      841 gcattagcaa attcgcgccc cactatgaat agttcgaatc gatctgtgaa tcgaggatct
      901 tctggatttt tcttcgctaa aggcgaaact tcgactggat gtccgtatac aaacgtcggt
      961 tgtgtcaatg tttcttcaac aaatgtttca aagaattcat tgataacatg tccgacaccc
     1021 atagcttcgg ttacttctac attgtgtttt tcggctaaag ctaatgcttc ttccaatgtc
     1081 atttcttgcc agaagtctac accagtttgt tctttgattg catctaccat atggatgcgt
     1141 ttaaagtcac tttctaagtc gacagcttgt ccatcatatg tgatttttgc tgttcctagt
     1201 actttttcag ctgcgttacg gataatacct tctgtcagat tcattacatc attgaaatca
     1261 gtatatgctg tgtatgcttc taacatcgta aattctggat tatgtgtagt atcaattcct
     1321 tcattacgga aaacgcggcc gatttcataa actttttcca ttccgccaac aattagtcgt
     1381 tttaaatgaa gttctagcgc aatacgcaaa tataaatcaa tgtccagtgc attatgatgc
     1441 gtgataaatg ggcgtgcggc agctccacct gcctcattgt gcaatactgg tgtttctact
     1501 tcaatatagc cattgccatc taaatagcga cggatttcgc tgataatttg gcttcgtttc
     1561 ataaatcggt caaagctttc gcgattactg attaaatcca agtagcgttg tctgtagcgt
     1621 tgttcaatat tcgttaagcc atggtattta tctggcagtg gacgaagtgc ttttgacaaa
     1681 atcgtgattt ctgaagcttt gatagaaact tcgcctgtgt ctgttttcat gatttgtcca
     1741 gttacaccaa agaaatctcc aaggtctgca tgtttgaata cttcataggc ctcatcacct
     1801 acttggtctt ttcttacata aatttgaatt tggccttcac gatcttgtag atgcgcaaat
     1861 ccagctttcc cttttcctcg gcgagtcatc atacgaccag ctacactagc ggtcaatccc
     1921 atttctgcta gttcttcttt tgttcttgga tcaaatagtt catgcaattc ttctgagttg
     1981 tgcgtgcgtt caaatcgttt tccaaatgga tcgattccat cttcacgcaa ttgttccatt
     2041 ttttgacgac gtacaagcat ttgatcattt aaatcttcta cttgatgttg ttgttcctgt
     2101 gtcacaaaag ttcctccatt ctcatgttaa tctctttcct ataatgccaa tgaaacacaa
     2161 aaaaagcaag ccgaccttgg aatttcggct tgaaaattca cgaaaaactt agaaatcaca
     2221 acgtttgttt caatagtact ttttccgctt tttctacaaa ttgatccagc aggtcattga
     2281 tttcttgctg tttttctgtc tgattgattg ctactttcac tttcgctgct cgaggaatcc
     2341 cttttaaata ataagccgca tgtgttctga attctctaca agctactttt tctcctttga
     2401 gatctgctaa gcgttgtaaa tgaagtttag ctgtagcaat tttttcggct ggtgttggtt
     2461 caggaatcag ttcccctgtt tcgagataat gctgtgtgcg gtgaatcatc catggattgc
     2521 ccagcgctgc acgaccaatc atcacaccgt ctgcaccgac atagtcaagc atccattttg
     2581 catcttctgg cgttttgaca tccccgttcc ccataaatgg aatcgtcaaa tgttttttca
     2641 cttctttcaa tacttcccag tccgcttttc cttcatacat ctgaacccgt gtacgtccat
     2701 gcatagcgat ggcacttgct ccagctgatt gtgctgctaa tgcattttct acagcaaaga
     2761 cgtgctcttc atcccaacca atacgcatct tgattgttac tgggatattt actgctgaag
     2821 aaacagcttg gaccatttcg tatactttct caggatctaa aagccatttt gctcctgctt
     2881 ctgctttgat tactttattt actggacagc ccatattgat atctattata tcggcagctg
     2941 tgttttcttc gacaaattga gcagcttcaa ctagactatc tttatttccg ccaaatatct
     3001 gtaaacttaa tggatgttct gtttcttcaa tgtggagcat ctctaaagtt tttttgtttc
     3061 tgaaatgaat accttggtca ctgatcattt cacatacgac taagcccgca ccaaattctt
     3121 tgacagtgac tcgaaatgct gaatttgtta ttccagccat cggtgcaaca acgacacgat
     3181 tagggatttc cacattgcca attttccatg tgtgttccac ttaaatccct cctaaggctt
     3241 ctttcttcaa tccatctaat tcttcttcgc tatacttgta tttgttatta cagaaagaac
     3301 aaaccgcttc tgcaccgtga tcttcgtcta tcattgcttg gatttcttct gcacctaacg
     3361 cgataattgc ttctgcaaat ttttcttttg aacagtcaca tttgaattga acaggcattt
     3421 tttccaaaat gttcaattct tctgtaccaa gcaatccttt caatatttct tctggtgtct
     3481 gtccttcatc taagagggta gagattcttg gtgtcgtttt taattgttct tcgatcttgc
     3541 tgattgtttc ttcatcggca cctggcatga tttggatcat gaatcctccc gccgtcttga
     3601 tgctgtcgtc agtattgacc aatacactca gtccaacagc tgaagggatt tgttctgaga
     3661 tagcaagata gtaagtaaaa tcttcagcga tttctccaga aacaatcggc gtttgcccag
     3721 aaaaaggttc ttttaatcct aagtcttttg tgacagtaaa cattccttgt gttccaactg
     3781 ctccccgcac atcgatttta ccttcactat ttagaggaag actgagatgc ggatttttga
     3841 tgtaaccttt cacatctccc tttccatttg catctactac aattcctgtt gctggaccat
     3901 cgccttggat tcgaacagtt agtttgtctt ctcctttgag cgtcgcacca agtagcagac
     3961 taccgaccaa tgtccgtccc aaagcagctg aggaggtgtg ccaagtatca tgacgtcttt
     4021 gtgcctccgc tacggtattt gttgcttgaa cggcgtatgc tcggacgaat ccgtcgtgag
     4081 caagtgcctt aaccaaataa tctttcattt gttatgtact cctttttccg tttttaaact
     4141 catagtgaat catactagca actactgctt ttttcaactt tggaatgctt cttttaaaaa
     4201 taattcagaa tcatctagaa aacaggacat caccctattt acaacttaaa taaagaaatc
     4261 tcccgaaaac cggtttttgt tccggttttc gggagtcata tcagatcaat tgacctatat
     4321 ttttttattt gtattgatcg tctgaatgat tatcttctga ttgttgagaa tctgaatcat
     4381 gttctgcttt attttcctca ttcagttcat gttttgcttc ttcaaaatca cgtttttctt
     4441 ccgcttgttt ttcagcgtct ttttcttcca aagcacgttt tgcttcttca aaagttttcg
     4501 cttctttttc gcttggataa tcatttgact cagtaccttc aggcatcaca ccgtgttcaa
     4561 acaatgactt gattgctttg gcatccaatg tttcgaattc caaaagtttt tcagcaatca
     4621 gtttatgctg tgcacgatga gcttcgatga tttctcgagc tttatcatga gcttccatca
     4681 agatttttcg tacttcttga tcaatctcga aagcgacttg ttccgagtaa gcttttgttt
     4741 gaccatagtc gcggccaaca aacacttgat ggtttccttc atattgaaca ggacctaaac
     4801 gatcactcat tccatattct gttaccatgc ttcgtgctag agctgtcgct tgctcaaagt
     4861 cattggaagc accagttgat tggacattga agatgatttc ttcagctgta cgtccaccaa
     4921 gtaagcctac gatttgttca aacatatctt ctttcgtcat caagaactga tcttcttttg
     4981 gtaaagcgat catatatccg ccggcgcgtc cgcgagggat gatcgttact ttgtggacga
     5041 cccgagcgcg actcaatact aatccgacaa tcgtatgacc agcttcgtga taagcaacca
     5101 tttcgcgttc ttttttgctg attacgcgat cttttttagc tggacctgca atcacgcgat
     5161 cttccgcttc atcgatatca gaagcatcga ttttcttttt atttcttcta gcagctacta
     5221 acgctgcttc attcaagaca ttttctaaat ctgctccggc aaaacctggt gtctgttgag
     5281 ccacaacttt caagtcaacg tcatctgcca tcggtttatt ccgtgcatgg actcgcaaga
     5341 ttgcttcacg accttttaca tctggacgtc caactaaaat ctggcggtca aaacggcctg
     5401 gacgtagtaa cgctgggtct agtacatcag aacggttggt tgcagcaatg acgattactc
     5461 cttcatttcc gtcgaagcca tccatttcta caagcaattg gttcaatgtt tgttcgcgtt
     5521 catcatgtcc gccgcccata ccggcaccac gttgacgacc taccgcatca atttcatcaa
     5581 tgaaaatgat agctggtgcg tttttcttcg ctgtttcaaa caaatcacga acacgacttg
     5641 ctccaacacc gacaaacatt tctacgaaat cagaacccga gatcgagtaa aacggtactc
     5701 cagcttctcc ggcaacagct tttgctaata aggttttacc tgttcctgga ggtccttcta
     5761 aaagtacacc tgccgggata cgggcaccta attcaacaaa tcggcgagga tctttcagaa
     5821 attcaacaac ttcaactagt tcttgttttt cttcttccgc acccgctaca tcagaaaaac
     5881 gtacgcgatt agcttttttg tctgcttctt tggcttttga tttaccaaag ttcatgacac
     5941 ggccgccacc gccaccgccg ccgccttgtt gtcccatcat catgtaaaag aaaaagatga
     6001 tgattacgat tggcaagaag ctaagcaata gggaaaccca tataccgctg cttgattctt
     6061 cttttactgt tgtagcaact ttgttttctt ttgccagttc tgttacttgg ctcaatgttg
     6121 tatcatttgg caaaatgatt gttgtgaaat gggtagtagt cgtatctgtt gaacctaaga
     6181 tagctaaccc accgctgtta gcgacggtct gctgttcttt gtattcacca gtgatcttat
     6241 atacaccatt tgcaggctgc acttcaaatt ctttgatctt gcctgcttgt agttgttctg
     6301 tgaattttga gtactcgata tctgacgatc gctggccgtt attaccaaaa atgaaataaa
     6361 cgaccattac catcgctaag attacgagga cataatacaa ggcatttttc atcatgccgt
     6421 tgttcttttt gttcatagtt gccctcccta agcgtcaatt taatttaaca gttttttatc
     6481 aatcctgacc attctattgt accataattt aattgagata cagcattaat ttgattgata
     6541 aacactaggt ttcaatacac cgatgtacgg aaggttacga tatgcttctg cataatccaa
     6601 gccataaccg accacaaatt catttggtac gttgaagcca acataatctg cttcgatatt
     6661 tactactctt ccttcaggtt tgtcaagtag tgtaacaatt ttgactgatt ttgctttgcg
     6721 gtatttgaaa agatccacta aataagcaag tgttcttccg ctgtcaatga tatcttcaac
     6781 gatcaacaga tcgcgacctt ctacattcgt atctaagtct ttgacgattt ttacttcccc
     6841 tgacgagact gttgcattcc cgtagcttga aacatccatg aaatccaatt ccaaatacgt
     6901 atcgatagaa cgaacgatat ctgccatgaa tggtaccgct ccttttaaga cacctactac
     6961 taggggattg gtgttttggt aaatttctgt taactctttg cctagttctt tgcaacgaac
     7021 ttgtatctct tcttgtgaga tcaaaattct ctcaatatcc ttttccaaca tcaggttctc
     7081 cttccaattg cttctttttg atatttgtaa agaagtatgt agtgtatttt atcagtttct
     7141 tcacgaatac tcaaatatga aaaggcaaaa ggtatcaagc tatatatatt tcttttggca
     7201 tctgtaatga tccaagcatg ctgcctttgt gaattaggta ctttctgatc gataaaatat
     7261 ctagatactt tttttgtgag attctctgtt aatttaatgc ggtctcctgc ttttcttttt
     7321 tcgacataca gttgcacatc agcaggaatc caaatgtcat gagaaaatag agaccatgaa
     7381 tctgttgctt tgatgtcccc tgcattttca ggtttgacca gaccaatcca ttctgtttca
     7441 ttcaaatata ttccttgttc tggaatcagc aacaagcgct ggtcttctgt ctggctagtt
     7501 gcttgttttt cttttttaga gagtacaaca aaatgatact ctcttttcag tatccaatta
     7561 ttgccaatac tgagttgcca ttggcttttt ttctgttgcc actggtctaa aatcgactcc
     7621 atttgttgtt ctttcaaaat aattcctgtt tttgagaatg ctttgtcgaa aaaggagtaa
     7681 aaaaaatagt atcgttcagc atctgtcatt tcttcaatca tttctgcaga aaatcgaaat
     7741 tgttcctccc actgttcgac ttccttttcg atcaactgtc ccatatattt ttgaatgact
     7801 tggtctgccc attggatctg ttgcgaaaat tgctggaaat gtctcaacgc ctgtgtattt
     7861 tcttgtttca attgaggaac taccaaatga cgaagccgat ttctctggat atctaacaat
     7921 tggtttgtct gatcctcaaa gtaaacaagc tgcgattccg ctgcataagt atataggtct
     7981 tctttgctaa aactcagcaa cggacgaatc agtctgcccg tagcaaatgg ttgtacttct
     8041 ttgattccag aaaaagtatt taactgtccc ccacgaacga ttttcattaa caccgtctcg
     8101 atttgatcat ctccgtgatg agcagtcatc aacgtgtcat attgtccttc ttccatcacg
     8161 cgagtgaatt cttgataacg aaagttacga gcttctgttt ctatggcgga tttggctgga
     8221 tcttcccaaa tacttacata taaagggaga ttcttctctt cacaatatga gcgaagatag
     8281 gcagcttctt ctgctgattc ttctcttaat tggtgattga tgtggacgat acctaaagaa
     8341 aaaccgattt ggcgttgcac ttgtaaaagt aaatggagca aaaccatcga atcaacacca
     8401 cctgataccg ctgctaaaat tctcgatcct tgttgccaga atccttgttc attcccgtgc
     8461 cgaataaatg actgctgtaa tagcggtgct tttttcccgc ctttttcaac gttcatcctt
     8521 tccacaccct ttcttcctaa aaaaaagaga gcgggaaaac gtctaaaacg acaattaacc
     8581 tgccctctgt tgatactatg acccaaatag tcgcactatc attatttgcg gctttttagc
     8641 tgcgacgtcc gccacggcca ccgcgctttc cttctgtgtt tcgcttcaat gaagataagc
     8701 ggtcatcgct atctttcaaa aatgaactca tcaatgaatc gaagtcttct ttttgagctg
     8761 aaggttgact gcgagaaaaa gattttttct gtgctggacg atttcggttg tcacggggat
     8821 ctttttgttg atatttttta ggacggcttt cttctccaga ttcagcaggt tgttcctgcg
     8881 ctttccggat agatagtccg atttttccat catcatttac tgaaataact ttaactgtta
     8941 cttcatctcc tacacttaaa acatcatgga tgtcttttac atagccattt gacacttcgc
     9001 taatatgaac tagacccgtt tttccagcac ctagatcaat aaacgcacca aaatttgtga
     9061 ttcctgatac ttttccttgc agttttgctc ctacctcgat tgacataaaa aaaatgttcc
     9121 tcctaattat ttcccttttt tatattcctg cttattttgc agttgtagaa ctagttgatt
     9181 gatctgtttc atttgtcgag ctgtctgtat ttgattctaa tataggataa actttttcac
     9241 cgtcttttga gtaaaagaat ttgcttctgg caagtttagc tacatactca tcatctttca
     9301 gcaatgaaac atcttggtgc agctgatcaa cgtctgcgtt gacttctttc atttcagtat
     9361 ttgcttcgac tctcaattcg ttcaattgat gcatcctttg catgtcatgg aaaatctgca
     9421 cacctattat cgcgaaaatg accaaagcac caacaaaaat tgcagccagt ctgcgtcttc
     9481 gaaaaatcag ttgacgttgt tgttgctgaa attcagcata ctttttcttt gcatattccg
     9541 tatctagcgc agcgatttta ttgctttttt tcgtcacttg gattcgctcc aatctttttg
     9601 attgtctttt tcattatacc aatatcgtga aagcttgtct aatctatttc tctagtgttt
     9661 ctgtgcgatt ttcagaaata atttcgtaca ttttttgtgc atcttctttt ttggttgatt
     9721 catgtaattc cgtaattttc acttccaacg ttttgttgcc aaactggact ttcaaaatat
     9781 cgcctacttt gacatcactg gaagatttcg ccaatttacc gttgatttga attcttcctt
     9841 tatcagctac ttcttttgct actgatcgtc ttttgatgat tcgggaaatt tttaaaaatt
     9901 tatctaatcg catatcttat tcctcacttt ttaatttttt tatttaatcg caggattttc
     9961 ttaccaaaag gcaacattag ccattctcgt actgtaaaca gacgaagacg aatcactgca
    10021 taaatgaaca cagaagcccc gatcccaact cctatcacgc tgacaaaaaa agcagtggaa
    10081 cgatgagtta caggaccaaa tactatattc aatacaccat aatacagcag cagtactact
    10141 gtcatacaaa gaaggctgaa catcaacttc cgtccaaaat gccgttcttt ccaaaaagca
    10201 tttagctctt ttgtttcaat tcgaacaaaa taccaaagcg tcacacctaa tcctaaaagg
    10261 gtagacaggc ttgcacctgc tgttcctaat aaaccggtca gaaaagaagt agtcaatcct
    10321 tttaccaata gtccccatcc tgcgcctttt aaagaagggc gaaacgagtt cttgctttga
    10381 gcaatacttt gataagcttg gatgactgca gtgaaagcaa tggaaaaaac aaataataca
    10441 agcgctgcat ttcccgcata atctttaaac aacgcataat tgatataagg gagcagcaag
    10501 gctaatccca aagacgcagc taatgctaaa gcggtcgtca atctaagata gattttggct
    10561 gtctgtaaaa actgacgata gatccggttg gtcaaatgac gagtcaacgc aggcaggaat
    10621 gtcgcactta atgcagtggc aatcactaaa cctaattgta ctaaaggctg acctcgatca
    10681 tagacccctt ttgctatctt cgcaccgtat tctgacagtc cgtaaacttc caaagcattt
    10741 tttacaaaga aagagtcgat cagctggaaa aagataagca atccactata aatcgaaacc
    10801 agtccgcctt ctaccagaaa acgccggatc aagggctttg acggctgttt gaacaatgga
    10861 aaccgtcgga agcttaacgc tcctccatga atcttttgtt catagtagtg aaggatccca
    10921 tatgcgcata aaccgccaaa aacagctcca ctcattgctg ccgtccctgt ttggtacacc
    10981 gtccatccaa gctttctaaa agcaaacgca ctacagacaa tcacaaaaac acgaacgaac
    11041 tgctcaacta cttgagaaac agcagttggt tccatcaaga atcttccttg gaaattccct
    11101 cgataaaaag atagaccagg catcagcaaa aaggtaaagg aaactacttg gatcagcggt
    11161 ttcaattgta tgtctcccat catccaagca atccagctgc taaatgcaaa gacaaacaac
    11221 cacaaaaaga cacctgtcca aaatactaag ggataaagat tttgcaaagc ttgctttcgc
    11281 tcgaaaggat tctttctttc tgcaacgtac tttgatatga actgcggaag accggataaa
    11341 gctaaagtca ttgctatccc ataaattgga taaacttgct gataaacata aaaaccttcg
    11401 tcgccgacta agttttggag gggaattcgg taaagggcac ttaatacttt tgctatgaaa
    11461 gaagcaatcg tcaatacaaa tgctccttgc atgacgcgcc gcatttcttt atgatccaaa
    11521 tcattgctcc ttcctacgct gacagatatt tttgctcacg caacgctttg acaaactttt
    11581 ggatttcctg aagccatact gcttcttcca tttggttggg gatcgtcaag cgtatcacca
    11641 tctgttcttt ttcaacgcct agactggctt ttagttttgt cgctgtcagt gcttcgaata
    11701 actgttccac actatagcgt tttgttccaa ctttacttag agtaaagact atatgcgcct
    11761 cctgtttcct tatcgtttct aggagcgctc gatcgccatc cattttgatc tgcccaattg
    11821 tcaagagatg tgctacttct tctggatatt ctccaaaacg gtcgagcaga tcatcttcta
    11881 gttcatctag catttcttgc gaatcaagtt cacgaattcg tttatagatc tcgattttct
    11941 gccgttgatc tgccacgtat gtttcaggta gatacgcatc gacaccgagg tcgatctcaa
    12001 cagtggtctt ctccacttga ctattctttc cttgtttacg agaaacagct tcgcttagca
    12061 tttgagaata catatcgaaa ccgactgcat caataaatcc atgctgctga gcacctaaga
    12121 gattacctgc accgcgaatg gaaagatccc gcatcgcaat cttgaatcca gatcccagtt
    12181 ctgtaaaatc tttgattgcc tgaagacgtt tttcactgac ttcgttaagg atcttctgct
    12241 gctcatacat aaagtaagca tatgcgacac ggctgcttct tcctacccgc cctcgcaatt
    12301 ggtataacgt ggataatccc atataatcag cgttctcaac gaacaacgta tttgcgttcg
    12361 ggatatctac tcctgtttca atgatggttg tagtgacaag aacatcatat tgaccttcaa
    12421 taaattcaaa aagcgtattt tctaattgcg cctctgtcat ttgcccatga gcataaccga
    12481 tccgtgcttc cggaacgagt tcttggattt cttctacttt ttgttcgatc gtttctaccc
    12541 gattatataa atagaatact tgtccacctc gtcccatttc tcgatggatt gcttcgcgaa
    12601 tagccccagg atttttttcc ataacatacg tttggatcgg ataacgattc gcaggcggtg
    12661 tttcgattac tgaaagatcc cgcacgccta acatcgacat atgcaaagtt ctcggaatcg
    12721 gtgttgcagt cagagtcaaa acatccactt gtgcacgtaa ctgtttcaat cgttccttat
    12781 gttttacacc aaaacgttgt tcttcgtcaa tgatcagtag acccaaatcg ctaaattcaa
    12841 tatcttttga caaaattcgg tgggttccta caacaatatc aacttgacct gtacgcagtt
    12901 gttcgatggt ttctttttgt tgctttcttg ttctgaaacg acttaatagc ccgatattta
    12961 cggggaaacc ttcaaatctt tccagcatcg tttcatagtg ctgctgtgcc aagatcgttg
    13021 ttggaactaa aaaggctact tgtttgcttt cctttatcgc tttaaaggcc gctcgaagcg
    13081 ctacttctgt tttcccataa ccaacatcac ctacgagtag tcgatccatt ggtttttctt
    13141 tttccatatc ccgtttgatc tccgctgcac ttctcaactg gtcatcagtt tcagagtagg
    13201 ggaaggcatt ttcgaattct ttttggtagg cgtcatcagg tccaaaagca tatccttttt
    13261 ctgactctct tgcggcatat aatttgatta gatcgtcagc gatatcctcg atttttgaag
    13321 agacttttcg cttggttttc gtccattcac ttccgcctag cttattgatg cgaggcgctt
    13381 tggcttcaga ggcgacatat ttttggatca gattcagttg agtaacagga ataaacagtt
    13441 tgtcgtcgtt ttgatacaaa atagtcatat agtcttggtg aacgccatct acttctagcg
    13501 tttccatccc aatgtatttc ccgataccat gattggcatg gacaacgtaa tctcctgctt
    13561 ttaactcatt atagcttttc aatcgctctg cgtttgttac tgtttgacgt ctcgtttgtt
    13621 ttttcgtcgt tttttggaaa atttcttttt ctgtgatagc tactattttt tcttgaggaa
    13681 gttcaaaacc agcctgcaaa gctccttcaa ctaattgtac ctgtcctctg atcaatgcat
    13741 ttttttctgt ttctactact aatatgtctt catctcgaag catttgttct gctttgcgta
    13801 ttctgtcctt tgtcggaata aagatcacaa cagtctgttt ttgcttgcgc caacggtcca
    13861 tttctgtttt cagcaatggc atttgtccga agaactgttg catcgtccga tattgaaaat
    13921 tatggattgc ttggaaacga agatttccca tccctttttg gaagagtgag aaaaaggttg
    13981 tggtgaactt ttccttctgc acgatttttt gtatatctgc tgaaaatgtc tgctcagaaa
    14041 acacccgcaa ttcgctgatt ttttgagtct gccattctgc cgcttccctt tcgatttccc
    14101 gatttgtttc catgattcgc tgataatcat caacgactag taaactattt gaagggaaat
    14161 aatccaagat cgtcacagat tcttggtaaa gaagatctga gtagaatttt gcttcttctg
    14221 tcgggacacc tgcttgccag cttgaagtca gctgaccgaa gtaatcttgc aaaaactcgc
    14281 ggtctgcttc ttctgtggtc gcagcaagcc gtttttctag cattttttgc atttgctcta
    14341 ttccatgttc tagatcctct ggtccgtaga cttgttcact cgttggggaa acccagattt
    14401 cttccgtttt ttcgattgag cgttgtgttt ctgcttcaaa ataccgcata gaatctatct
    14461 ctacatcaaa tagttctaca cgaacaggat aggttgtcgt taacgggtaa atatcgataa
    14521 tgcttcctcg aatactgaat tctcccggtt ttgcgaccat ctcttctcgt acatagccca
    14581 tcaaagtcaa ttggcgagga agattttcca cgtctatctc atcaccaata tgccaattca
    14641 attgcgcctg tagccaagtt ttcttattcg gcagtcgttt gtgcaaagat gcgactggca
    14701 acaaatagat acctgctttt ttttcttgaa gtgcgttaag cgttgcaact ctctcgcctc
    14761 gcgcttctgg agaagaaaag gccatttcag cagaaagcac ttcatccact ggaaataaat
    14821 ggatctgatc ttgcggaatc acatgttgca gatcttctgc taactgattc gcataataca
    14881 aatttggcat cagaataatc atgtttttat ttgttttctt aaacgctcga gcaaatagaa
    14941 gtgttttcgc tgagcccgct aatcctgtga ttagttgtct aggcattttt tcttctaaat
    15001 tgctgaccca ttcttttact tgcggatctt ttccgactaa ttgaataata tccattcctt
    15061 ttcctccttc ctcaattgaa tcgattcatc gcatccacga acgaatgccc ttctatgaga
    15121 tattcgactg ctttaacaga ttgacacatg ctttcttcta tcaaaggctg attttctttg
    15181 ctgaaaggac taagcacatg ctggactact gtttttttcc cttctggcct gccgattccc
    15241 accttgattc gatcaaagac attcgtattc aaatgggaaa taatactttt gataccgtta
    15301 tgcccgccgg cacttccctt ttgacggagc cggatttttc ccactgccag atccaaatca
    15361 tcgtagatta cgactaattc ctctggataa atcccaaaat aagtcattag tgggccgact
    15421 gcacgaccag attcattcat gaatgtttgt ggtttgacta aaaggatctt ttctccatta
    15481 tgaaaaaatt cggcaacttc tgcttcgaac ggattttttt tgaaagtagc ttgatgctcg
    15541 cgtgcaattt tatccacgac cataaagccg atattgtgtt ttgtatattg gtatttggta
    15601 cctgggtttc ctaacccgac gatcattttc atttttgttt tccacctaat tctaaaaagc
    15661 agcacaaaag gctggactgc taattagcgg tccagtccat cgagcttttt tcttttttac
    15721 acttgtcaat gcatagtata acacaaaaca aggcgtttcg gctaaaagag aatcgttaat
    15781 aaatgataaa gaaattaaaa atttcttcaa agtttaatga aataattgat atataaaaat
    15841 agtgaaataa cgcacatgta agcttttaca tataatacaa ccttgttatc tgttatattt
    15901 tatagccaaa tagcgtgaca tggggcttga gaaatgttag cataagcatg ggaaaaaaca
    15961 aatcaatgga aggattgata ctaaatgact gcagctgcag aaaaaaaaga tcatcaaaaa
    16021 gttattcttg ttggagacgg cgccgtaggt tctagctatg cctttgctct agttactcaa
    16081 aatatcgctc aagaagtagg tattgtggat atcaatactg ctaaaactga aggagacgca
    16141 attgacttgt ctcacgcttt ggcatttact tcacctaaaa aaatctattc tgctacttac
    16201 gcagacgcac atgatgctga cttagttgtt attacagctg gcgcaccaca aaaaccaggt
    16261 gaaactcgtt tagacttagt aaacaaaaac ctaaaaatca accgcgatgt tgtcacacaa
    16321 atcgttgaat caggatttaa cggtatcttt ttagttgccg ctaacccagt agatattttg
    16381 acttactcaa cttggaaatt ctcaggtttc cctaaagaac gcgtaatcgg ttcaggtacc
    16441 tctcttgact cagctcgttt ccgtcaagca cttgcggaat tagttgacgt tgatgcacgt
    16501 aacgtccatg cttacatctt aggtgaacac ggagattcag aattcccagt ttggtcacat
    16561 gcaaatgttg ccggcttgca aatctatgaa tgggtaaaaa acaacccaga aatcgatgaa
    16621 gaagctatgg ttaacctatt ctttggcgta cgtgacgctg cttacacaat tatcgagaaa
    16681 aaaggcgcta ctttctatgg tatcgcagta gcattagcac gtatcactcg tgccatctta
    16741 gatgatgaaa atgctgttct tcctttatca gtattcatga atggcgaata tggattgaac
    16801 gacatctaca ttggtgcacc tgctgttatc aaccgtcaag gtatccaaaa agtaatcgaa
    16861 attcctttaa gtgacagcga gcaagaccgt atggctgctt ctgctaagca attaaaagaa
    16921 attctagatg aagcttttgc taaactagac gctgaataat ctttgaacat gacggaaaac
    16981 atctattcgg tgttttccgt ttttttattt tatcaccctt atggtttcta aattttttca
    17041 taaagtagag ataaccttcg tcttttcctc taaattatgc tatcatgaga ccgttccacc
    17101 ctaaactttt acttaaggag ttgtcgatat gacaagatct tcgcatcgaa aaaaatcgaa
    17161 acatgttgct ttgctttcag ttcttggtgt tctcttagtg ctgatcagct tttataccta
    17221 tcgcagtaca tactacacaa agcatttttt gccgaataca gaaataaatg gaataaatgt
    17281 cagcaatcta acagtcgaaa aagcaaatga aaaattaaaa gaagcttatt ctgataaaaa
    17341 actggctatt aaagaagatg gaaaagtctg gcaggaagta ccaaaatccg agctcggcta
    17401 caaagatgat ttcacttcgg agctttctcc gatcctaaat gaacaaaacg gatggacttg
    17461 gggaatgact tatgtctcag cggcagaaaa acaagaaata gatcctgtca gtcaaaacca
    17521 ccaaaaacta gacactaccg ttcagacatt gacaactaag ctgactgatt tgaacaaaga
    17581 ccggacacct actactgatg caacgatcga aaaagcaggt gactctttcg ttatcaagcc
    17641 tgaagtcaat ggaaatacga tcgacgtcga tgctgcggtc aaacaattaa aaagtgcggt
    17701 aaactctggt aaagatacaa ttgaattgac tgaattcaaa gaaaaaccaa aagtaacatc
    17761 tgaagacagc tcactgaaag aacaactcgc atccatgaat gctatcgcaa atgtaaaagc
    17821 tacttacagt ataaacggtg agactttcca gatcccatca agtgatatta tgagttggct
    17881 gacctacaat gatggaaaag tcgatttaga tacagaacaa gtacgtcaat atgtaacaga
    17941 tttaggcaca aagtataaca caagtacgaa tgacacaaaa tttaaaagta caaaaagagg
    18001 agaagttacg gtcccagttg gcacttacag ttggacgatc caaacagaca gtgagacaga
    18061 agcattgaaa aaagcgattc tggcaggcca agattttact cgttcaccca ttgtgcaagg
    18121 tggcacaacc gctgatcatc cattgatcga agatacgtat atcgaagttg atctggagaa
    18181 ccagcatatg tggtattaca aagacggaaa agtggccctt gaaacagata tcgtttccgg
    18241 aaagccaact acacctacac ctgctggagt cttctatgtt tggaataaag aagaagatgc
    18301 aactttaaaa ggaacaaatg acgatggaac accttacgag agtccggtaa actactggat
    18361 gccgattgac tggacaggtg ttggtatcca cgattcagac tggcaacctg aatacggtgg
    18421 agacttatgg aaaacccgcg gttcacacgg ctgcatcaac accccaccaa gtgtaatgaa
    18481 ggaattattc gggatggtcg aaaaaggaac gcctgtattg gtcttctaac aaaaataata
    18541 tataaaaaga aagcacccgt acgattggta caggtgcttt ctttttatta tctaacaatg
    18601 tgctacttac atttcagcag cagtaaagac ttctgaaaca tcatcatcgt cttctagctt
    18661 atctactaga cgttccaatt gctctttttg ttcctcattt aaatcaatcg tcgtttgtgg
    18721 aatcatcgtc aattctgctt gcgccaaagt gaactctttt tccaactcat ctcgtacagc
    18781 agcaaaatct tctggtgcag tataaatctc gaatacttca ggagaagttt gcagatcttc
    18841 tgcccccgcc tctaatacat cctcaaacat cgtctcttca tcaacagtca acccatcgcg
    18901 ttcaatagcg atatatcctt tgcggtcaaa caagtaactg actgaacccg tttctcccag
    18961 tgatccaccg tttcgagtaa aagctactcg gacatttgtc gctgtccgat tacggttatc
    19021 tgtcaatgca tggactaaga ttcctactcc acccggacca tatccttcat acgtgatttc
    19081 atcgtaattc gtttcatcta cggcactcgt agccttttta atcgcccgtt ccacattgtc
    19141 gttaggcata tttgcagctt ttgctttgtc cacaacaagc cgcaaagctg gattcataga
    19201 tggatcagga ccgcctgctt tagctgccat ataaatttca cgagaaagtt tctgaaagac
    19261 tttccctcgt tttgcatctt gggcattttt tcttccttgg atattatgcc atttactatg
    19321 tcctgacatg atttttcctc ccttgttctt ttgagaatac acttctattc tagtactatt
    19381 tcacaaaaaa acaaagccac agccaatcta taaacgcttc ttgaatgccg caaaaagcac
    19441 acataatatc acagcagtca gagcaccgat cgaatccaac atgacatctt ggaacatagg
    19501 tgttcgtcca ccggtcatca tctgatggaa ttcgtccaaa cctgcgtacc ctgttgctga
    19561 aagccaagca aggatcgctg tcagccacca aatttttagc ttggggaaaa cacctaaaaa
    19621 caagctgcct cccaaaataa aataggtacc gaaatgtgcg cccttcctta aaaagaactc
    19681 taaaaatttg gcgtagccca gatggttgat actcaccata cttccaccat aattaaaacg
    19741 tatacccgct aatgtgtctt taaatggctg attaggcagc cagctttcta atcgcgaaac
    19801 ttgtgattgc tgtccgtaag tttgagaaga actgtaaaat aagattataa cgactatcag
    19861 tgcgattact aaataaatat ttccgttttt catttgtttt tttgaagaat tcataagtca
    19921 cctctgtatt ttatcttaga ctttttttga agaaacaaca atcattttca tagagacttc
    19981 ttccataaaa aagtgctata cttaattttg tattacaata tgaagggagt tttttcatga
    20041 gttattcatt aaattggaat ctagattcta ttttttccgg aggcagtcat tctgatgctt
    20101 tgaaccaacg gatgaagcaa ttggaagacc aaatgaacga gtattatcac cgcgtaacaa
    20161 aatggtcacc tagttctgac aatgcagagc agttaaacgc tattcttcaa ttgcaagaaa
    20221 cgattacgaa tggttttacc caatgcagca gctatattac tgcattgctt tctgccaatg
    20281 tcaatgattc agatgcaaag gtcttatcag gcaagcttta cgcaatgctg cctcgtctgc
    20341 aatcagccga aactgtattg tcaaagaaat ttgcagaaat atctgacaat gattggaatc
    20401 agatgctttc tcaatcttcg tttgaaacaa ttgcttttcg tttaaacgag attcgtcgtg
    20461 atggctcaca attattatca gaagctgaag aaaacattat aaatacgctt tctttagatg
    20521 gtctaaatgc ttggagcagt cattatgata cgattgtagc cagcatttcg attcccttcg
    20581 aacaagatgg acaagtagtc gaattatctg ctggacaagc cttcaataag atgatgggcg
    20641 atccagatcc aaaagtcaga gaaacacttt tcgcagcatg ggaagaagcc tggaaagaaa
    20701 aggcacccct cttcacggat actttgaatc atttagatgg ttttagactt tctgactatg
    20761 aacttcatgg tgtcacagat tttctacaaa aaccattaga gtacaatcgg ttgaagaaag
    20821 aaacattgtc tgtcatgtgg gatacaatcc agaaaaacaa acaacccctt gttaattact
    20881 tgactagaaa agctaatctt ttcggtaaag aaaaaatgga atggcaagat caagatgcac
    20941 ctattatttt aggcgatttg aaagaaaaaa cctttagttt tgacgaagca gcagcattta
    21001 ttatcgaaaa ctttaacaaa ttcagtccaa aaatggctaa atttgcccaa tctgcttttg
    21061 aaaagagctg gatcgaagca gaagatcgtc ctgggaaacg tcctggtggt tattgtactg
    21121 agttaccaga aacaaaagaa tcaaggatct ttatgaccta cagtaattct gttaatgagg
    21181 ttgcgacact tgctcatgaa ctaggacatg ctttccatag cagcacgatg tgggatttac
    21241 cttctttaaa tcgcgaatat gccatgaatg tcgcagaaac agcaagtaca ttcgcagaat
    21301 tgatcgtagc cgatgctaca ttaaaagtcg caaaaacaaa agaagaaaaa atcaatttgc
    21361 tagatacaaa actgcaaaat gctttagcta tgttcatgaa tatccattcc cgattcattt
    21421 ttgaaaatcg attctatgaa gcacggcaag aaggattagt ctcagaagat aagatcacag
    21481 agatgatggt tgaggcgcaa aaagaaggct atcaaggagc tttagctact taccatccat
    21541 atttctgggc tgcaaaattg catttcttta ttgatgacgt accgttttat aacttcccat
    21601 acacatttgg ctacctcttt agtttgggca tctatgctta cgccaaccaa aagggagcaa
    21661 attttgaaga agaatatatc catttactac gcgatactgc ctcaatgacg acggaagatc
    21721 ttgcgaagaa acatttgaat gtcgatctta cccaaccaga tttttggcaa gcaggtatcg
    21781 acatggtgac tgaagatatc caaacgttca tggaattgac agaagaattt atctgatttc
    21841 tattttatca caataaaaaa agagttagtc cagaatgggc taactctttt tg
//
LOCUS       DAYYGS010000052        21793 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000052 DAYYGS010000000
VERSION     DAYYGS010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.052"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(541..1566)
                     /locus_tag="ACHA44_001768"
     CDS             complement(541..1566)
                     /locus_tag="ACHA44_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905040.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1841..3526
                     /locus_tag="ACHA44_001769"
     CDS             1841..3526
                     /locus_tag="ACHA44_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFM8905041.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGTRVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3540..4823
                     /locus_tag="ACHA44_001770"
     CDS             3540..4823
                     /locus_tag="ACHA44_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905042.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4893..5762
                     /locus_tag="ACHA44_001771"
     CDS             4893..5762
                     /locus_tag="ACHA44_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905043.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5759..6595
                     /locus_tag="ACHA44_001772"
     CDS             5759..6595
                     /locus_tag="ACHA44_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905044.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6621..7688
                     /locus_tag="ACHA44_001773"
     CDS             6621..7688
                     /locus_tag="ACHA44_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFM8905045.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7685..8512
                     /locus_tag="ACHA44_001774"
     CDS             7685..8512
                     /locus_tag="ACHA44_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFM8905046.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8557..9582
                     /locus_tag="ACHA44_001775"
     CDS             8557..9582
                     /locus_tag="ACHA44_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8905047.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9579..11762
                     /locus_tag="ACHA44_001776"
     CDS             9579..11762
                     /locus_tag="ACHA44_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HFM8905048.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11762..12892
                     /locus_tag="ACHA44_001777"
     CDS             11762..12892
                     /locus_tag="ACHA44_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8905049.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHA44_001778"
     CDS             12889..13566
                     /gene="pgmB"
                     /locus_tag="ACHA44_001778"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFM8905050.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13650..14177
                     /gene="msrA"
                     /locus_tag="ACHA44_001779"
     CDS             13650..14177
                     /gene="msrA"
                     /locus_tag="ACHA44_001779"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8905051.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14378..15064)
                     /locus_tag="ACHA44_001780"
     CDS             complement(14378..15064)
                     /locus_tag="ACHA44_001780"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFM8905052.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15144..16682
                     /locus_tag="ACHA44_001781"
     CDS             15144..16682
                     /locus_tag="ACHA44_001781"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFM8905053.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16802..17269
                     /locus_tag="ACHA44_001782"
     CDS             16802..17269
                     /locus_tag="ACHA44_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HFM8905054.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            17803..18231
                     /gene="rplM"
                     /locus_tag="ACHA44_001783"
     CDS             17803..18231
                     /gene="rplM"
                     /locus_tag="ACHA44_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFM8905055.1"
                     /translation="MAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPTFTPHL
                     DTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLIETSVK
                     GMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHA44_001784"
     CDS             18247..18639
                     /gene="rpsI"
                     /locus_tag="ACHA44_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8905056.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(18727..19953)
                     /locus_tag="ACHA44_001785"
     CDS             complement(18727..19953)
                     /locus_tag="ACHA44_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905057.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(20025..20327)
                     /locus_tag="ACHA44_001786"
     CDS             complement(20025..20327)
                     /locus_tag="ACHA44_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003013637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905058.1"
                     /translation="MTIAANNDTSVRLAVNWGEVKFLCAYPKQFKNFITTILGDDFIE
                     SRVYLNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKDFMNTFKIT"
     gene            20875..21594
                     /locus_tag="ACHA44_001787"
     CDS             20875..21594
                     /locus_tag="ACHA44_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8905059.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            21596..>21793
                     /locus_tag="ACHA44_001788"
     CDS             21596..>21793
                     /locus_tag="ACHA44_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFM8905060.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINF"
BASE COUNT         7075 a         3842 c         4514 g         6362 t
ORIGIN      
        1 gtctaaaata atttgtctaa ctagaagaaa agtgggaaag gatcttaaca acatcggttc
       61 gtatccaatt aaggtatcgg aacgatcatc ttccaagaga agtcaatcaa caaaagtaaa
      121 gatagatcat tttttgaatg caaaataaat tgatgggaag atagagcggt tatttttaat
      181 attttgctag ctattattga ataattcctc gaataaaaca aaaccagtag acatgtctgt
      241 tattctattt atgttttaga gataagtatc cccaaataac atggattgaa ataagggtaa
      301 atgtgtttaa aaaaacgaac tataaaaact tatacacaac gtggttaaaa atagtttttt
      361 cttccctgtg gaaaaaatag tttaacacaa acattatagt tatccacagc tatctaaaac
      421 agatggaaaa taagaaaaat tcgctaaaat accgggaggg ggtaacgagg gatgagtgat
      481 tgaaaaaaga acagagtaac tgacttatcc tcagtgtttc tgttctttta taattctgtt
      541 ctacatttct tgttgttgga tcgaatctgt gagagtaatc tcggttgaaa gaatgattct
      601 tttttcttca gggtattggt ggtggattag ttggaataaa agttctgtgg ctttgattcc
      661 taactgattt gtcggaatgt ctatccgagt caactgtggc gaaaataggg aggacaataa
      721 gggatcactg aatcctgcga tcgaaagatc atcaggtatc tgctttccag attgttctgc
      781 cactttgtaa atactcaatg attttaatgc atcggtagaa aaaatcgcag ttggtctttc
      841 tcgtagcttc attaaatcat tcattgctgc catgctatct tctaagcgaa agccgccgtc
      901 tataatccat tcattacgga tatctaggtg gtgatcaaat aagcattgac ggtaaccatt
      961 gagacgatca ttggatacat aaacttctgt cggagcatgg agacagccga tatgacggtg
     1021 tccattcttg atcagtgttt ccaccaaatc gtaactatcc tggaaattat ccgtgtccac
     1081 tgagtagatg ttcgtgtagt cgtttccgac ttttccgatt accacaatag gaatattgta
     1141 ttgatccaat tcaaaaagaa gtgtttcatc catcgtagag cttaaaataa taattcctcg
     1201 gatcattttt tcttggatct ttttcgcgca tttttctagc tcttcttctg gtgtttttgc
     1261 cgtttgtaga atcaagtcga agttattttg ttcagaaacg gcagaaatgg attgaataac
     1321 atctgaaaaa aaaggattgc cataggtcgt atgagaagta cgatgagata atgctagaat
     1381 cgcgtcaaaa ccatttcctg acagtcctct tgcaattttg ctcggttcat aattcaattc
     1441 tttgattgct tgaagtacac ttgcttttgc tttatcagag atatttggct ggttatttaa
     1501 tactctggag acagttgatt ttgagacatt agcttttttc gcaatgtcat aaatagtagg
     1561 tttcaatttt atatgcacct cctttttggg agcgttttag aataattcca tagtacaata
     1621 gacattcttt tttgtctatg attttgaaaa tttaggaata gcttgcatga tcatgagaga
     1681 ccatattttt ttaagaagct attaattatt gatacataag ctttcttatt tgtatttttg
     1741 tgtatatcaa aattatttaa aaaaatgcta ttgacagtaa gcgttttcca taatatgatc
     1801 aaatcgttgg gaacgctccc aaaaaagaaa ggggaatccg atgtatacaa aacttttaca
     1861 agcgatttat cgcgactcag atttgtctga gaaaaccgcc tctatgattg aacaaaaagt
     1921 cagtgtttat cagcagaaga aaggccaggc aggcaaactg cattgggacg aagaagatat
     1981 tttcttgatt acttatgggg atcaattttt tgaagagcag caaccaactt tgcgtacatt
     2041 taagaaattt tatcaaaagt atttggcaga tacatttaga gttgtacatt tcttgccatt
     2101 tttcccatat acatctgatg atggtttctc cgttgtaaat tatcaggcaa tcaacgaccg
     2161 tttgggcaca tgggaagata tagaagaaat gagaagcgaa acacggttaa tgttcgactt
     2221 tgtctgcaat catatgtctg ccgaaagtac ttggtttcag gaatatttaa acttgaatga
     2281 tacgtacaaa gattttttta ttgatttgcc actggataca gatacaagca gcgtgactag
     2341 accaaggacc agcccgttac ttacaacgtt cattgacaaa aacgggaaag aaaaacacgt
     2401 atggacgact tttagcgaag atcaagtaga cttgaatttt aaaaatccag aagttttatt
     2461 gaaaatgatc gatattcttt tgttttactt agaaaaaggc gcttcattta ttcgcttgga
     2521 cgctgtggga tttttatgga aagaactagg aacttcttgt atacacctgc cagaaacgca
     2581 taaaatcatc caattattcc gtgcaatcgc tgatgaagca gctccaggaa cggtattgat
     2641 cactgagaca aatgtgccac acaaagataa tatcagttat tttgggaatg gcgagaatga
     2701 agcacagatg gtctatcagt tttcgctacc acctttgacc cttcaagcga ttcgttcggg
     2761 taatacaagt aagttgaaag aatggttaaa aaccatcgat ttcgcatcga aaaaaacaac
     2821 tttttttaat tttttagcct cccatgacgg tatcggattg aatccggtac gaggtattct
     2881 gccggaaaaa acgatccttg aattagtgga acagctgaag aaagagggta cccgagtaaa
     2941 ctacaagaaa aacccagatg gaacgaccag tccatatgag ataaacgcta cgtatctaga
     3001 tgcgttaagt tctccaacag atacgacgga atggagaagc aaacgctttt tagctgctca
     3061 ttcatttttg gtaacgttac caggcgtgcc ggcaatttat attcaaagtg tgctgggaag
     3121 tcgaaatgat tacaaaggcg tagaaaaaac aggagagaat cgaaaaatca atcggcaaaa
     3181 atttttgtta gcagatattg agaaagagtt gcagacagat ccattaagaa cagcagtata
     3241 tggtgaattg acccagatgc tatctattcg aaaaaaagaa aaagccttcc atccgaatag
     3301 tttgatttac ttactggact cagcagattc ttttatcagt tttattcgcg aagcgaagga
     3361 agagaaagtc ttagttatcc ataatttttc caatcaatca gcaggttatc ttttaccaaa
     3421 aggagattac ctagacctgt tttctggaca aattatccat tgcagtcaag aacgaaagtt
     3481 gatattccag ccgtatgaat atatgtggct gaaaataata aaataagagg tggaaaaaaa
     3541 tgaaaaaaat tattgttact acctgtctgc tagctactgg atttcttctt actgcttgtg
     3601 gtgaaagtca aagctctaac gaatcacaac agacaacgat tcaattcatg cactcttctg
     3661 tcgaacagga acgattaagt gtgatcaatg aactggttgc tgattttgaa aaagaaaatc
     3721 cagacatcaa aatcaagcaa gtgccggtag aagaatcagc gttcaataca aaaatcgtta
     3781 cgttagctcg ttccggtaaa ttaccagaag taatggaagt cagtcaggat tttgctaaag
     3841 tgatggataa agatgaactg attgatcaga cggcagtcca aaacgttatc gaggaagctg
     3901 gagaagataa ttattatgac ggagccaaaa accttgttcg ttcagaagat ggaaaaagct
     3961 atattgctgc accaattagc ggctgggttc aaggtatttg gtatgacaaa gaaaaattat
     4021 cagaagctgg tttttcagaa ccagaaaatt gggatgatat tttaaaaatt gctcaacatt
     4081 tcacagataa agagaataaa caatatggaa ttgctatgcc tactgctgaa ggaacaatga
     4141 gtgagcaagc gttctctcaa tttgctttgt caaatggagc aaatgttttg gatgatgaag
     4201 gcgaggtaac gatcgatacg gaaaagatga gagaagcatt gtcgttttat caaaacttat
     4261 cccaatacac gatgccaggc tctaatgatg tgacagaaat caaagatgct ttcatgaatg
     4321 gaacagcacc aatggcaatc tattcaactt acattttgcc ttctgtttat gaagcgggga
     4381 actcagaaaa tatcggtttt gctatcccaa cacaaaaaga tcaagctgtc tatggcacag
     4441 tatctggatt gacgatttct tctggtctag acgagaaaca aaaagaggct gctgaagcat
     4501 tcacagcttt cttatctcaa ccgaaaaaca tggaaaagtg ggtcttgatg tctcctggtg
     4561 gtgctcaacc agtgaataaa caagtagtcg aactggatgg ttacaaagaa aatgaagtca
     4621 tcaagtcttt tggcgagctg ccatctgaga ttgctagtgc ttttgatgaa gtacaagtct
     4681 ttggactagt cggggaaaag aatttcacga aaatgggaga tatcacaagc tcaggtgtga
     4741 tcggaaaagc agtgaaccaa gtaactgtcg gaaacgaaga tgtcgatcaa gctttagctg
     4801 acgcacaaaa aaatacgaaa taacaagaag ttctgccaat ggtgttcgaa aaaagtagat
     4861 agctattggc agattatcag ggggaggttt caatgaatcg tccaaggaaa cattctgact
     4921 taaaattagc catgggactt ttagcaccaa gcatgctttt attagctgtt ttagtgctga
     4981 ttccaatgct ttccaatatt cagatcagtt ttttcgatat gccgctgaat cctaactcag
     5041 acgccgtttt tatcggatta agcaattatc ttgaaatttt aagtgatagt gaattttatc
     5101 gttctttagg tttaacagtt gcttacacat tcttagttgt agcaggcagt acagctttag
     5161 gattagccgt agctattttg cttaaccgca agttcagatt taggaaaacg gtgcgttcac
     5221 tgattttatt aagttatgtt acgccatcta tttctttgat ttttgcttgg aaatatatgt
     5281 tcaataatag ttatggaatc gtcaatgatc tgttagtaga taggttgcag cttttcaacg
     5341 aagcaccgct gtggttcgac cagccagttg ctagcttctt tatggtcgtt ttgtttgcta
     5401 tctggagata ctatccgtat gcgtttattt catttttggc tattttgcaa acagtcgaca
     5461 aaacgcttta tgaagcagct gaaatggacg gtgcaaatgc ttggcagaag ttcaagatcg
     5521 tgacactgcc ggcaatcaaa ccagtactag caactgtgat tgttttacga tctatctgga
     5581 tgttctatat gtttacagat gtctatctac tgacgaataa agtcaatatc ttaggcgttt
     5641 atttgtatca gacagccttt gcgttcaatg atcttggaaa agcagcagct atatcgattc
     5701 tattgttcat cattattgca gcggtcgtct tgctctcacg aaagaaggtg gacttgaaat
     5761 gaaaaataaa aagagcagga aaatcttatt ttacgcgacg attttgttgc tattagctgt
     5821 caatttgttt ccatttttga tcatgctgat gacatcattc aaggattcac aagaagcaat
     5881 tgcaacgaca caaactattt ttccgaaaga atggactttt gaacattatc aggatatttt
     5941 caatgctgag atttttccat tcatcatgta tttcaaaaat agtttagtga tctccttgat
     6001 agcctccgta actgcagtag tgattgggat cagcggggca tacgctttgt caaaactacg
     6061 cttcaaagct cgctcgaaaa tcaatgctag cttttacacc gtttatatgt tttccggtat
     6121 tttgatcgtt gttccattat ttaaaatcat ttccggtcta ggtttgtatg ataccaatac
     6181 agcactgatt atcacgatgg tcgttcaaac acttcctact gcgatattca tgctaaaaag
     6241 ttattttgac acgattccga ctgatattga agaagcagca atgatcgatg gtcttagccg
     6301 gatccagatc attattcgga tcattatacc attggctatt tctggaatca tttctgtctt
     6361 cgtttattgc tttatggttt cgtggaatga ttatttattt gcttcgattt tcctgtcgac
     6421 ctccgaaaaa tttactttac caatcggttt gaacacatta ttcagtacac cagactacat
     6481 ttggggacga atgatggccg cttcattggt gaccgcttta ccagtcatca ttatgtatgc
     6541 gttatctgaa cgatttatta aaggtgattt agtcgaaggc ggcgtaaaag gataaaaaga
     6601 aaagaaagag gaaagaaaag atgaaaaaat tagttgcatt agaaccaaga atagccggat
     6661 tcaaagatta cgaagaccgt gagatcgaaa agaacgaagt acgtatccga gtaattttcg
     6721 ctactccaaa accaggaaca gaaattgtcg actttagagg attaagtcca tttatcgatg
     6781 aagaattttc agaagaatgg cgcctattct tgccaagaga agaaggaagc caacgcggtg
     6841 tggtatttgg cgaatttcaa ttaggaaata tgattgtcgg tacgatcgaa gaaacgggca
     6901 gtttggtgac agagtatcaa aaaggagagt tagtctgcac ttatggaccc attcgtgaaa
     6961 cgctgattac aaacggtgtt gataattata aactacgtaa acttgatcgg ttggatcaat
     7021 ggaaaaatgc agtatgttac gatcctgctc aattcgcttt aggtggtatc cgggaagcac
     7081 atgttcgagc aggtgatgct gtagcaatct tcggtttagg cgctatcggg caaattgcga
     7141 ttcaattagc taaaaaagca ggtgctacta ccatcgttgg gatcgatccc atagcgcagc
     7201 gtagagaatt agcagaaaaa tacggtgccg attacacgct agatcctatg aaagcagatg
     7261 tcggttttcg tctaaaggaa ttgacagata aacgtggagt agattcagtt attgaaacga
     7321 gtggcagtgc tgccgcatta caagcttctt taagagggat cggctatggc gggatcattt
     7381 cttatgttgc ctttgctaaa ccttttccag ctgggtttaa tcttggtcgc gaagcacatt
     7441 tcaataatgc aaaaatcgtc tttgctcgag catctagtga accaaaccca gattacccaa
     7501 gatggaaccg taaacgaatc gaggacacat gttgggaatt attgatgaac ggttacttgg
     7561 attgtgaaga aatcattgat ccagttgtgc catttgcaga aagcgcagaa gcttacatgc
     7621 gttatgtaga ccagcaccca gaagaaagta tcaaaatggg cgttcaagtc cgaggaggag
     7681 aacaatgaaa ttcggtacgc aagatcaagc atttttccca agagacattt tagaaaaatt
     7741 tagatttatc aaagactgtg gatttgaaga atacgaaatc gacggtaagt tgttaataga
     7801 aaatatcgac acagtcaaac aggcaatcaa agaaacaggg atcccagtag tgacagcttg
     7861 taacggctac gatgggtgga tcggtgactt tatcgaagaa cggcgtctta atggtgtacg
     7921 tcaaattgct gagattttgc gtgcactaag cgaggttgga ggcaaaggaa tcgttatccc
     7981 agcagcttgg ggaatgttca cttatcgatt accgccgatg gtttcacccc gaagcaaaga
     8041 aggagactat aaagcagtca gcgaatcact tcgttattta gataaagtag ccgaagaaac
     8101 aggtacggtt atttatttgg agcctttgaa ccgataccaa gaccatatga tcaatttgtt
     8161 agcggatgcg cgtcattaca ttgactctaa tcaattgaaa catgtgcaga tcatcgctga
     8221 tttttatcat atgaatattg aagaagatga tatttcaaaa gcattgacta cgcaaaaaga
     8281 gtatgtgggt catatccatt tagcagataa tcatcgctac caaccaggat ctggcgcaat
     8341 tgattttact cgccattttc aaacgctact tcaaaatgag tatgaaggtt atatggtata
     8401 tgaatgtcgt ttgcgaggag aaaatgaagt gaaaatttat caggaatcat tgcaatactt
     8461 gaaaaactgt ctggaaacag cgcgtcattt aaatgattta gagaaagaat agagaaagaa
     8521 taacaataag taaataagca aaggagggag aaataaatga agaaagtagc aatcataggc
     8581 gctggacaag tagcagaaaa agtacatgct gcttattatc aaaaaagatc agatgtctta
     8641 gaattggccg ctgtggttga tccagtcagc cagcgagcag aagacttttg ccgacgtaca
     8701 ggattttctc atgcgtatac atcagttaca caaatgctga atgagatttc tcccgatatc
     8761 gtgagtgtct gtacacccaa tcgctttcac ttcgaacatg tattggctgc cctcgaagct
     8821 aatgcttcag tgatgtgcga gaaaccacca gctatgacag ctgatcaagc aagaaagatg
     8881 gcagagacag ccgataaaaa cgggcagatt ttggcttatg gattccagca tcgtttttct
     8941 gacgaagcaa caatgatcaa gaatcatctt gatgttctcg gggatattta ttacgtagaa
     9001 gcgaatgcgc ttcgtcggtc tggtgttcca ggctggggaa cgtttatcga caaagaactt
     9061 caaggaggcg ggcctttgat cgattatggc attcacatgc ttgatacagc gatgtatatt
     9121 actggatttc ctaaagtaaa aagcgttcgg gcgtacagct tccaaaaaat tggtacgaaa
     9181 aaaaattcag ggaaattcgg gaaatgggat ccaagcaaat tcacagtaga agatgcaatt
     9241 tttggcacac tggaattaga aaacggcaag atcatccaac tgaatacttc gtttgctttg
     9301 aatatcagag aagacaaact gaatgtggct ttttgtggcg atcaggcagg tgctaatctg
     9361 tatccaggcg aactattcga agatgtagat ggaaagctgg tttccttaca aaagaccacg
     9421 ccagttaatg acccacatga actaagtatg gctcattttg tgaatcgggc gctcggtgac
     9481 cagactcaga tgattgcaga tggctggcaa ggatatgtca tccagaatct ggtggaaaat
     9541 ttatatcgtt cggcagaaac aggagaagtt attaccttat gaaagtagaa gaaaaacagt
     9601 ttgatttaga gtatatcaat aagtatgcga gtcagatgac cattggaaat ggattttttg
     9661 gtatcagagg aagccaggag gaagactatc ggaatcaagt aaggggaatg tttgcagctg
     9721 gtgtatataa ccgaccttta ggagcagaat cagccgagtt agtgaatctc ccagatgtca
     9781 tcagatggga aataaaaatc gacggtgaac atttttctct tgagacgatg aaagttcttg
     9841 catacgagcg ggcactagat ttagaaatgg gagagcttaa acgctatgcg ctcattgaaa
     9901 ctgctcaagg aaaaaaagtg gaagtcatta cttgtcgtgt tgctgatcaa aataatctcc
     9961 atcaggcagc cctgaaagtg gtcatcaagc cgttgaccga agactgccaa gtagatgtga
    10021 aaacgggtat cgatggacag caaaccaatt ttggtacaca gcagttggtt gaaaaagaac
    10081 tgcgagtatt tggtgacgat ttgatcagtg catcctacca gacaacagaa agtaaaattg
    10141 atattgggat tgcagctaaa ttcaataaag caggtgtatt ccaagcaaaa aatcgccgga
    10201 tcaccgcttc cttttcagaa aagatcagaa aaaatacgtc ctttacgctg gagaagatca
    10261 tcgtgctctc tacctctctt cagcaaacag acccgtttaa gaaaagtatt gcccaagccc
    10321 agaaaagcgt tagttataca gaaattttag agcagtcgat gtcttattgg acagattttt
    10381 ggcatcagca tcggataacg atccaaggcg attcttcttt cgatcagtta gcgatggact
    10441 tcgctgctta ccatttacag attatgactc cccgcaatga tccaagtgtt agtgtagggg
    10501 cgaagggact aactggcgaa ggttataaag ggcatgtttt ctgggatacg gaaattttta
    10561 tcctgccttt ctttttatat cagtttcctg aagtagcaaa gaatttgctg ctgtaccgtt
    10621 atgatcgatt agaaggcgca cggcaaaaag caagagatta tcactatcaa ggtgcacttt
    10681 tcccatggga aagcgcacac actggtcagg aagagacacc caaatttgct gcattgaata
    10741 tcaaaaccgg aacaagacaa gaagttgctt cagctgccgc agaacatcat atcagtgcag
    10801 accttgctta tgccgttcaa gagtatgtcc aagcaacaga ggatcaatcg tttttagaaa
    10861 caaagggaaa aacgctcatt catgaaacag cagctttttg gctgagcaga gctgtaaaag
    10921 tcgatgatca tttagagata cacagagtca ttggtccaga tgaatacaca gaatatatcg
    10981 acaataatgc atatacgaac tatctggcgc attataatgt ggccttagct gttaaatatc
    11041 aagctggcga tccgcttttt ttgaaagaat gtcaaaattt tttagataag ttatatttgc
    11101 cagtgcctaa caaagaacag ctgattccgc aagatgacac gtatctcagt aagccagtta
    11161 ttgatttgac cacttacaaa gaacgtcctg gcagccagtc tattttactt gattatgccc
    11221 gtagcgaagt aaatgaactc caagtcagca agcaagcaga tcttgtcatg ctgttatact
    11281 tattacctga gtatttttct caaccaatcg tttctaagaa tttgcattac tatgaaaaca
    11341 aaacaatcca cgactcatcg ttgagtaaag cgattcacgc catagaggct tgccgtatcc
    11401 aagaaatgga atgggcgtat catttattcc aagaagcgtg cctgattgat ttaggaccag
    11461 atccgcattc atcagatgac ggaattcatg ctgcttcatt aggagcacta tggctagcag
    11521 ttatatttgg tttttcaggt attacagtaa cgaatggaac tctttctatt tctccaagac
    11581 ttccttcagc atgggaaaaa atttcttttc cttttatttg gaaaaatcac aggttgctat
    11641 ttgttttgac aaaaaaacaa atagtaataa aaaaagagac gaaagaaaca ttacctattt
    11701 ttgtcaatgg agaccaatac cagctggaaa aagaactgat cttggaacgg aggggaaaat
    11761 aatgcgtatt ctgattgcgc cagattcttt caaagaaagt ctgacagcct tagaggcagc
    11821 acaagccatt caatcaggtt ttcaagaagt ttttgttcag gctgaatatg atctgctacc
    11881 tataggcgat ggaggagaag gaacggtttc atcattagca gacagtttaa ggttgaagcg
    11941 gcaaacagca gtcgttacta atgcatttgg caaaaacaaa gaaatccagt acgcttcaga
    12001 cggtcaaact gcgatttttg aaatggcaga aatcgtcgga ttgaaagatg tcccactaaa
    12061 ccaacaacag ccacttaggt tatccacgcg aggagtagga gaactgatac gacaattgtt
    12121 gcaacaaggt gttcgacaca tcatcatcgg cgttggcgga agtagtacca acgatggcgg
    12181 catcggcatg gcagaaggtc taggttatgc tttttacgat caagataatc aaaaagtaga
    12241 agcactggga gaaaatctag gtaaaattgc tacagttgat aaaacgaacg tgcctattga
    12301 gctgattaaa gccaaaatca ctgtcattac tgatgttact aatcttttat gcggaaaaga
    12361 aggcgcttct tttgttttcg gaccacaaaa aggcttgaaa gaaaatgaat tagcagtggc
    12421 tgaccaagcc atgcatgatt tttatatgag atttgcccct caagtaatcg aggcatcggg
    12481 cagtagtgct ggtggtggaa tgggtgcagg actgttagca tttgcttcag ggaaaatagt
    12541 ctctggcatc gactacgtat tggatttact ggagttcgat aaacgggtac agcaagcaga
    12601 cctcgtaatc gtaggcgaag gacgtctaga tggacaaagt ctaaaaggaa aagcgccagt
    12661 tgggattgct agaagagtac cagagggcgt tcctgtgata gctatttgcg gaagtgttgg
    12721 taaaggaagc gaaaaagcag cagatgttgg catttcggcc gtttttccga tccttcctgc
    12781 agctgtgcct ttacaagaag ctttacagca agccactgat aatcttcatc gcacagctaa
    12841 aaacatcgca tctttacttc aagtcgttca ttttcaagga ggtaatcgat gaaaatcaaa
    12901 gcagcactat tcgatttaga tggcgttctt gtagacacag ctcgttatca ttacgaagcg
    12961 tggctggttt tagctaacca gctgtccatt ccattcactg agaaagaaaa cgagcaactg
    13021 aaaggaatat ccagaaccga gtcactagag cgattgctgt catttggaaa aatggaacag
    13081 aaattctcag aaaaagagaa atcagccttt gctgagcaaa aaaacaatct atatcttcaa
    13141 gcgattcaaa aaatggatga aacatccgtt ttgcctggtg caatagctgt tcttgagtac
    13201 ttgaaaaaaa caaacatcaa gatcggattg ggatcagcaa gcaaaaatgc acgcctcatt
    13261 ttagaaaaaa cgaatttgac aagttatttt gatgtcctta ttgacggcac gcaagtatca
    13321 aaagcaaaac ctgatccaga agtttttctc aaaggagcac agcaattgaa tgtaccgcca
    13381 aatgcatgct tggtcattga agattcagaa gctggttgtc aagcagcttt ggctggtaac
    13441 atgcatgtat tgggaatagg tgaaaacata aatcttcctt ctgctgaata tgtgattcct
    13501 gatttaaccg tttttgatca agtacgttcg ttttggcatt tatcagaagc cgtatcacgt
    13561 atataatagt ttccgttttt tctgtcacaa gcaaaacgtt atcctccttt tggaaatcaa
    13621 ggtatgatga gaaaaaaggg ggattttata tgacaaaaaa agcagttttc gcaggcggat
    13681 gcttttggtg tatggtcaaa ccatttgaag agcagccagg gatcatttct gtgacatccg
    13741 gttatactgg cgggcatgta ccaaatccaa cttatgaaca agtaacaaca ggcacaacag
    13801 ggcatacaga agcagtagag atcgaatatg atccagaagt tatttcttat gatcagttgg
    13861 tggaaatcta ttggcagcaa accgatccga ctgacgcatt aggacagttc gctgatcgcg
    13921 gtgattctta tcgtccagtg atctactata atgatgaaga agaacgaaat attgcagagc
    13981 gttcaaaaca agcattgcaa gacagcgggc gattcgataa gccaatcgtg acaaaaatcg
    14041 aaccaagaag cgaattttat cctgcagaag attaccatca ggattattac aagaaaaaca
    14101 agctccatta caatctttat cgagaaggtt ctggacgtgc aggtttcctt cgtcgacatt
    14161 ggggcgtcaa gaaataagaa gccgaaaaag ctacttatgg aacaccgtat atcagaggtt
    14221 gtaaaaccag cattttgagc taatactgag ggtcagctga ttgaacacca tttagtggag
    14281 aggttgtgaa cctgtcgcct agctccaagt cacaaggatt tggtaagaga acactatata
    14341 tcagaggttg tgacaagtcg tttgtcacaa ccttttttca atacctaaga tgaaggacaa
    14401 tcagcaacgc actgctcaaa aaaggaatga aaggcagctt ttccaacttt tttttatgac
    14461 agataaagta aacagaaaaa acggatattc caaacagact agcaagaaac aagatctgaa
    14521 gcaactcttc tagagacaag ccaccactcc aagaaagcat caacagagta tcgccagctc
    14581 cgagatagga ttgaccatag cgaaggcaca caccgcctac gataaacgtt aacagcacac
    14641 tttgccattg gaagacgccg gcctgcaacc aaaaaatcca taaaattgcc caagaacaga
    14701 atagcgtgtg agaatggacc atgaagtaaa ataaatccat caacgaaaat agcaaagccg
    14761 aaagcatcca tatgaacgtc gaaaaatttc ttgcggaaga aaaagtaaag aaccaggcaa
    14821 aaagaccacc acataaggct tctgccaaca aatagtagaa gggcaaccga cacctgcaat
    14881 agcggcagcg aaatcgttgg acgatgatcg agagaatcgg aatcaattca taccaagaaa
    14941 gcgaacgaca gcatttgaca caatgagatc taggataaat aatagagtga cctaacggaa
    15001 tccgctgggc ggttaaacat aaaaatgatc cgatacagca accaatgata aaataaagta
    15061 acacgccatc tccccctttt caaaaccaag atacgcaagc ttaaactaaa tttttgtaca
    15121 tacataaaat ttgaggtgaa aaaatgaacg tttcaatcgg attagatatc ggaaccacac
    15181 aaaccaaagc agttgctttc acagatgaag cccaagaagc agcatccgct tattttcgct
    15241 accctttgat ccaagaaaca caagggatgg cagaacaaga tcaggaattg atctttcaag
    15301 gtgtctgcac cgttataaaa gaagtaatta aacagttgcc ttcagacaca atcattcaat
    15361 ttgtctcctt ctcctctgcg atgcatggcc tgatattgtt ggatgaagag aaaacaccgt
    15421 tatctcgaat gatcacttgg gcagataacc gagcagcgga cgaagcagaa aaactgaaat
    15481 ataccgtgct tggcaaagaa ttttatcggc agactgggac gccgattcat ccaatgacac
    15541 cattgaacaa aatcaagtgg ctgcaaaaca gccaacctga attattcaaa aaaactgctt
    15601 attttgtcgg tatcaaagac tatatctttt atagattgtt tggcgaactg atcagcgact
    15661 acggaacagc ttcaggaact ggatattttg atatccatga attccaatgg gcagaagaag
    15721 tgcttgacta cttgaacatt cgaatagagc aattgccgca agtctttcct tccacctatc
    15781 aagtaacagg tctaccatca aaaacagcag cagaactagg tttgccaaat gatatatcat
    15841 ttgttttggg aggggcagac gggccgcttt ctaatctagg gttaggtgct tttggtgata
    15901 cgatcgccgc tttgaccgta ggaacgagcg gagcacttcg ttttattact actcagcctc
    15961 agttccatcc agaaatggag acgttttgtt atgttctgga tcagaaacat tgggtagtgg
    16021 gaggagcgac tagcaatggt gctggcatct ttgattgggc agggcataca tttttacagg
    16081 aagtcgttca atctgctaaa gaaaaaggag aaaatccata tgatgcactg ctttcggcta
    16141 tcgcttttgt gccagcgggt gccaatggtc tgatttttca tccctattta cttggtgagc
    16201 gagcaccgct ttgggacgca gatgcgtttg gcagttttat cggtttacag cggcaacata
    16261 cagaaaaaga aatgatgaag gcgattctag aagggatctg tctcaacctt tatcggattt
    16321 taaaaggagt ttgccaggaa aatcagctca cagagatccg tgctacaggc gggtttgctc
    16381 aatctccgat ttttcgtcag atgatggctg atgtactggg ttacccgctt gttttcccta
    16441 ctgtcacaga agcatccgca ttaggagctg tgattttagg ctggagaagc atcggaaaga
    16501 tccaaagtat cagtgaagca caggaattga tagaattgaa agagcaagtt ggagtacaag
    16561 aaactgatca taaaaaatat cagaaaatct atccattatt tgtttcgaca caaaagcatt
    16621 tagcacagac ttatcagcaa tttgctcaac tgcgagaaga attagctgat tctgaaaaat
    16681 gattggcttt acggataaaa aagcgaactg aaaatcgttc tcaggtaaat gattttgttg
    16741 tacttttcat tgaaagtcta ctattatttt aagtgtaaga aaatgttagg aggcaagaaa
    16801 gatgaaattc caacaaacca aagaagtatt aaatcaatta gttgcagatc taagccaaat
    16861 gtctgttgtt gttcaccaaa cacattggta catgagagga ccaggtttcc ttacattgca
    16921 cccaatgatg gatgagttca tggatgattt gaacgctcag ttagatgaag tatccgaacg
    16981 cttgatcaca ttagacggtt caccttattc tacattgcgt gaatttgcag ataacacaaa
    17041 aatccctgat gaaattggcc gctgggatcg tactattcca gaacgtttgg aaacacttgt
    17101 tgcaggctat cgttatctag cagatcttta ccaaaaagga atcgaagttt ctggagaaga
    17161 aggcgacgat ccaacacaag atatctttat cgaattcaaa acagccacag aaaaacgtat
    17221 ctggatgatc caagctcatt tgggcaaagc gccagagatc gacgcgtaaa aactgttgtt
    17281 gaacttgacc gaaaaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    17341 acgtcaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagctgat
    17401 tgaacaccgt ttaccaagag gttgtaagcc tgccgttcag ctccgagtca caagaaacaa
    17461 ttttaaaaaa cagcttctta tgttttatgg atattgtgac ttgtgaccga ggagttggca
    17521 ggagaacacc gtttattcaa aataaggggt tgtgacaaga cttttgtcac agcctctttt
    17581 tgtttttcat tttgacagga aacgaccctt ttatataaaa gaaagaaaag aagtaaagtt
    17641 tcatataaat ttttcaatca agcagaaaaa cgttgacatt gactagatta gcgggtaata
    17701 tgttaaatgg tattgtttgc cccacaaaga gccccggaaa ctcgaagtgt atgtcaaaca
    17761 tcagaactta aattggagga aaataacgtg cgtacaacat atatggccaa accaggcgaa
    17821 gtagaacgta aatggtatgt agtagacgca actgatgttc cattgggacg tctttcaaca
    17881 gttgttgcat ctgtactacg tggtaaaaat aaaccaactt tcacacctca tttggatact
    17941 ggcgatttcg tcatcgtcat caatgcagac aaagtgaaat taacaggtaa aaaagcgact
    18001 gacaaaattt attaccgtca cagcaactac ccaggaggat tgaaatcaat cactgctggc
    18061 gaattgcgtg ctaaaaattc tcgtcgtttg atcgaaactt ctgtaaaagg tatgcttcct
    18121 aaaaacactt taggccgcaa acaatttaca aaattgaacg tatacggtgg agcagaacat
    18181 ccacatgcag cacaacaacc agaagtttta gacatcacaa acttaatcta ataggaggga
    18241 atttcattgg cacaagttca atatatcggc acaggccgtc gtaaaaacgc agttgctcgc
    18301 gtacgtctag taccaggaac tggcaaaatt actgtaaata aaaaagatgt tgaagaatac
    18361 attccacatg ctgacttgcg tgaagtaatc aatcaacctt tcgctgttac tgaaacaaaa
    18421 ggcgcatatg acgtatttgt taacgtaaac ggcggaggat acgctggaca atcaggagct
    18481 atccgtcatg gtatcgctcg tgcattgcta gaagttgatc ctgacttccg tttagctctt
    18541 aaacgtgctg gcttacttac tcgtgacgca cgtatggtcg aacgtaaaaa accaggtctt
    18601 aagaaagcac gtaaagcttc acaattctca aaacgttaat atcctttata tatcaacgtt
    18661 tctggcactt ttcaattatt tggaaagtgc ttcttttgtt tttaagaaca catttaagaa
    18721 cacatatcaa tcacacttat tcaatcctct ctgaaaagct tcaaaaacag ccttatcaac
    18781 tatatctggt gtgttcacat atatctttgt tgtagaaata tctgagtgtg ttaaagcatt
    18841 tgaaatttga ttcatatctg ctccaccttg tctagctata gttgcataag tatgacggag
    18901 tttgtgtgga gataaatgaa ctaagtcagg atgccttctt ttaattgaat tgattcgata
    18961 gtttaggtaa tcggcatgaa caggacaatt tacttctcca gatggtttag tataagtaaa
    19021 gagatactga tctaacgttt gcttaatttt taatttcaac aattgatcag cttgtaccga
    19081 tttccattca cttagcaatg tcattacaac ttctggtact tgaatgacgg tatcttttcg
    19141 accttttgta aacttttttc tttgatattt atctaaagta tgtttaagac ggaccgtttg
    19201 tttttcaaaa tcaatatatt tccattgcaa agcgtaagtc tcactttttc tatcaccaag
    19261 atacagggta atcataaaaa gtgtgtaatc atgaaaagta agtaaatgat tattaaaatc
    19321 atcatttact gcttctatcc aattagtaag ttcttttgat gatagtgctt gcttaccttc
    19381 ttgtattctt ttttcttcta aagcattttt tttaggtgca gtgatacttt gaataatttt
    19441 agtagttcga ttaaattcga tataatttag tatttctgca atatcgaaca tggaacgaac
    19501 atatcctttt atgattttta tatttgcata ttctttcgat tttttggtta attcatctga
    19561 tattatctca gtattgatat ttagataatt cattgcgtat tcaccaaaca taggtaaaat
    19621 atgcaaccga aatatatctt ttgtattact aatggttata tcactaggag gcctgtttga
    19681 tctaattgtt tgacctaatt catatctagg aagccattta gattcgtaaa attttttaaa
    19741 cgttattttt ccttttaatt ctaatgagtt tgcattctcc tctctcaaaa ctttttctat
    19801 tttcttcttc atatcatttt cggctttaat cgccaattgc ctatttggaa atgttttttg
    19861 atacttttct gatgcaattc ctgttatttc tttaaacctt tttggatatc tcagattagt
    19921 tttatatttc ccatttgaaa gctttgtagg caaaatcctc actccttaaa aaaagtaagg
    19981 cagtatattt cactaatttt agtatatgcg gcgagaagaa aatttcaagt tattttaaat
    20041 gtattcataa agtctttaat atcttctacg tcaaagcgaa ctacaccacc aactcttatt
    20101 actgggaaat tatatatacg aatccactta tctagagtat tattatcgat tcctagaaat
    20161 gcacaggttt gttttttatt caggtagact ctggattcta taaaatcatc tcctaaaata
    20221 gtagttatga aatttttgaa ttgctttggg taagcacata ggaatttcac ctctccccag
    20281 tttacggcga gccttacgga agtatcatta tttgctgcta ttgtcatggc attttgtttt
    20341 cccagaacaa aaagaaatct ctaagtattt gtcgctaatc aggtcatggc gtacttgagt
    20401 ttctagctcg aatagcaggg atcgttattc aaaaattgta tttaattgtc aatgatcaaa
    20461 aatagttttt ttcctattca ctattaataa cgataaacat gtataaaaag acacactacg
    20521 aaaaaagtta aatggagttt actgattttc atatttgtat atggaacaga catagtcttg
    20581 aataattttt ataaatgttt tgatgcttat caacttattc aacccgctat ccacttcaag
    20641 tataattttt ccatacgcca ataaagtagt tttaggagca tgttctcatt tgagaacgct
    20701 ccttttttat atattttgaa agctacttac tatacgcgta gtttcaaata gttttgtcat
    20761 tctaatcttc agcaacagaa aaaaatctaa actataaaat ttagggttga ctaaaatata
    20821 atttttgtat accatatgat cgtgataatc attataattt agaaggagaa agttatgtta
    20881 gaagtgaaaa agcttaatgt ttgttataac gattttttag cattgaacga gatttcttta
    20941 aaaatacaag aaggtgcttt aacaggtatt attggtccaa atggtgctgg aaaatccaca
    21001 ttgcttaaag caatgttaaa tattatccct catcagggag aggtctccat taataatgaa
    21061 gatatcaata aaaaactggt gaagattgca tatgtagagc aaaaagctga tattgatttt
    21121 acttttccaa taaaagtaaa agaatgtgta tctatgggaa catatgccgg aatgaaagtt
    21181 tttcaaagaa ttaaaaatgc agaatggcaa agagtttcaa aggctttgga aaaggtcgat
    21241 atgggaaaat attctaatca tcaaatagga gaattatctg gaggacaatt tcaacgtgta
    21301 cttttagcaa gatgtcttgc tcaaaatgcg gatttcatat ttttagatga accatttgta
    21361 gggatagact tagttagtga acgaataatt atggatacgt taaaaaaatt gaaacatcag
    21421 ggaaaaacag tgctaattgt gcatcatgat ttaagcaagg taaagaagta ttttgataat
    21481 attattcttt tgaatcgaaa tctaattgct cacggaagtg tagaatctgt atttaatgaa
    21541 gaaaatttaa aaaaagccta tggagatact atatttattg gagaggggga ataatatgct
    21601 tcaaaatttt attaatggtc tgtatgactt tcacttttta cagaatgctc taattacatc
    21661 tgtagttata gggattgttt ctggaacagt aggttgtttt attattttaa gaggaatgtc
    21721 tcttatgggt gatgctattt ctcatgctgt tttgccagga gtagcaattt cttatatttt
    21781 aggtattaat ttt
//
LOCUS       DAYYGS010000053        20503 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000053 DAYYGS010000000
VERSION     DAYYGS010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.053"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(105..629)
                     /locus_tag="ACHA44_001789"
     CDS             complement(105..629)
                     /locus_tag="ACHA44_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HFM8905061.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA44_001790"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="ACHA44_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFM8905062.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA44_001791"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="ACHA44_001791"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFM8905063.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="ACHA44_001792"
     CDS             complement(2351..3031)
                     /locus_tag="ACHA44_001792"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFM8905064.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="ACHA44_001793"
     CDS             3157..4026
                     /locus_tag="ACHA44_001793"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8905065.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="ACHA44_001794"
     CDS             4052..4567
                     /locus_tag="ACHA44_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HFM8905066.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="ACHA44_001795"
     CDS             complement(4614..5177)
                     /locus_tag="ACHA44_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905067.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="ACHA44_001796"
     CDS             complement(5232..5411)
                     /locus_tag="ACHA44_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905068.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="ACHA44_001797"
     CDS             complement(5488..5997)
                     /locus_tag="ACHA44_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905069.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="ACHA44_001798"
     CDS             complement(6093..6473)
                     /locus_tag="ACHA44_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8905070.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA44_001799"
     CDS             6803..7552
                     /gene="pnuC"
                     /locus_tag="ACHA44_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFM8905071.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="ACHA44_001800"
     CDS             complement(7736..9013)
                     /locus_tag="ACHA44_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905072.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA44_001801"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="ACHA44_001801"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HFM8905073.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="ACHA44_001802"
     CDS             complement(9935..10879)
                     /locus_tag="ACHA44_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HFM8905074.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="ACHA44_001803"
     CDS             complement(10881..11567)
                     /locus_tag="ACHA44_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HFM8905075.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="ACHA44_001804"
     CDS             12041..12361
                     /locus_tag="ACHA44_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HFM8905076.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="ACHA44_001805"
     CDS             12515..13420
                     /locus_tag="ACHA44_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905077.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="ACHA44_001806"
     CDS             13417..14190
                     /locus_tag="ACHA44_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8905078.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="ACHA44_001807"
     CDS             complement(14371..15795)
                     /locus_tag="ACHA44_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid/polyamine antiporter"
                     /protein_id="HFM8905079.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHA44_001808"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="ACHA44_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFM8905080.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="ACHA44_001809"
     CDS             complement(16517..18499)
                     /locus_tag="ACHA44_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose PTS transporter subunit IIA"
                     /protein_id="HFM8905081.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHA44_001810"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="ACHA44_001810"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFM8905082.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="ACHA44_001811"
     CDS             complement(19412..20473)
                     /locus_tag="ACHA44_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MupG family TIM beta-alpha barrel fold protein"
                     /protein_id="HFM8905083.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
BASE COUNT         5921 a         4347 c         3632 g         6603 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaataacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tca
//
LOCUS       DAYYGS010000054        20111 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000054 DAYYGS010000000
VERSION     DAYYGS010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.054"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            262..570
                     /gene="rpsJ"
                     /locus_tag="ACHA44_001812"
     CDS             262..570
                     /gene="rpsJ"
                     /locus_tag="ACHA44_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFM8905084.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            601..1230
                     /gene="rplC"
                     /locus_tag="ACHA44_001813"
     CDS             601..1230
                     /gene="rplC"
                     /locus_tag="ACHA44_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFM8905085.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1258..1881
                     /gene="rplD"
                     /locus_tag="ACHA44_001814"
     CDS             1258..1881
                     /gene="rplD"
                     /locus_tag="ACHA44_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFM8905086.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1881..2171
                     /gene="rplW"
                     /locus_tag="ACHA44_001815"
     CDS             1881..2171
                     /gene="rplW"
                     /locus_tag="ACHA44_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFM8905087.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2210..3043
                     /gene="rplB"
                     /locus_tag="ACHA44_001816"
     CDS             2210..3043
                     /gene="rplB"
                     /locus_tag="ACHA44_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFM8905088.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3086..3364
                     /gene="rpsS"
                     /locus_tag="ACHA44_001817"
     CDS             3086..3364
                     /gene="rpsS"
                     /locus_tag="ACHA44_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFM8905089.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3387..3734
                     /gene="rplV"
                     /locus_tag="ACHA44_001818"
     CDS             3387..3734
                     /gene="rplV"
                     /locus_tag="ACHA44_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFM8905090.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3748..4404
                     /gene="rpsC"
                     /locus_tag="ACHA44_001819"
     CDS             3748..4404
                     /gene="rpsC"
                     /locus_tag="ACHA44_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFM8905091.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4407..4841
                     /gene="rplP"
                     /locus_tag="ACHA44_001820"
     CDS             4407..4841
                     /gene="rplP"
                     /locus_tag="ACHA44_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011375443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFM8905092.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4831..5019
                     /gene="rpmC"
                     /locus_tag="ACHA44_001821"
     CDS             4831..5019
                     /gene="rpmC"
                     /locus_tag="ACHA44_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFM8905093.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5044..5310
                     /gene="rpsQ"
                     /locus_tag="ACHA44_001822"
     CDS             5044..5310
                     /gene="rpsQ"
                     /locus_tag="ACHA44_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFM8905094.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5368..5736
                     /gene="rplN"
                     /locus_tag="ACHA44_001823"
     CDS             5368..5736
                     /gene="rplN"
                     /locus_tag="ACHA44_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFM8905095.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5773..6081
                     /gene="rplX"
                     /locus_tag="ACHA44_001824"
     CDS             5773..6081
                     /gene="rplX"
                     /locus_tag="ACHA44_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFM8905096.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6109..6648
                     /gene="rplE"
                     /locus_tag="ACHA44_001825"
     CDS             6109..6648
                     /gene="rplE"
                     /locus_tag="ACHA44_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFM8905097.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6667..6852
                     /locus_tag="ACHA44_001826"
     CDS             6667..6852
                     /locus_tag="ACHA44_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HFM8905098.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6888..7286
                     /gene="rpsH"
                     /locus_tag="ACHA44_001827"
     CDS             6888..7286
                     /gene="rpsH"
                     /locus_tag="ACHA44_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFM8905099.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7318..7854
                     /gene="rplF"
                     /locus_tag="ACHA44_001828"
     CDS             7318..7854
                     /gene="rplF"
                     /locus_tag="ACHA44_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFM8905100.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8015..8371
                     /gene="rplR"
                     /locus_tag="ACHA44_001829"
     CDS             8015..8371
                     /gene="rplR"
                     /locus_tag="ACHA44_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFM8905101.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8392..8892
                     /gene="rpsE"
                     /locus_tag="ACHA44_001830"
     CDS             8392..8892
                     /gene="rpsE"
                     /locus_tag="ACHA44_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFM8905102.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8907..9086
                     /gene="rpmD"
                     /locus_tag="ACHA44_001831"
     CDS             8907..9086
                     /gene="rpmD"
                     /locus_tag="ACHA44_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFM8905103.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9130..9570
                     /gene="rplO"
                     /locus_tag="ACHA44_001832"
     CDS             9130..9570
                     /gene="rplO"
                     /locus_tag="ACHA44_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFM8905104.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9570..10865
                     /gene="secY"
                     /locus_tag="ACHA44_001833"
     CDS             9570..10865
                     /gene="secY"
                     /locus_tag="ACHA44_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFM8905105.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10927..11574
                     /locus_tag="ACHA44_001834"
     CDS             10927..11574
                     /locus_tag="ACHA44_001834"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFM8905106.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11765..11983
                     /gene="infA"
                     /locus_tag="ACHA44_001835"
     CDS             11765..11983
                     /gene="infA"
                     /locus_tag="ACHA44_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFM8905107.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12016..12132
                     /gene="rpmJ"
                     /locus_tag="ACHA44_001836"
     CDS             12016..12132
                     /gene="rpmJ"
                     /locus_tag="ACHA44_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFM8905108.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12150..12515
                     /gene="rpsM"
                     /locus_tag="ACHA44_001837"
     CDS             12150..12515
                     /gene="rpsM"
                     /locus_tag="ACHA44_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFM8905109.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12543..12932
                     /gene="rpsK"
                     /locus_tag="ACHA44_001838"
     CDS             12543..12932
                     /gene="rpsK"
                     /locus_tag="ACHA44_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFM8905110.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13012..13950
                     /locus_tag="ACHA44_001839"
     CDS             13012..13950
                     /locus_tag="ACHA44_001839"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFM8905111.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13978..14358
                     /gene="rplQ"
                     /locus_tag="ACHA44_001840"
     CDS             13978..14358
                     /gene="rplQ"
                     /locus_tag="ACHA44_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFM8905112.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14630..15469
                     /locus_tag="ACHA44_001841"
     CDS             14630..15469
                     /locus_tag="ACHA44_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8905113.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15445..16314
                     /locus_tag="ACHA44_001842"
     CDS             15445..16314
                     /locus_tag="ACHA44_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8905114.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16304..17101
                     /locus_tag="ACHA44_001843"
     CDS             16304..17101
                     /locus_tag="ACHA44_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8905115.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17304..18050
                     /gene="truA"
                     /locus_tag="ACHA44_001844"
     CDS             17304..18050
                     /gene="truA"
                     /locus_tag="ACHA44_001844"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8905116.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18714..18908
                     /locus_tag="ACHA44_001845"
                     /pseudo
     CDS             18714..18908
                     /locus_tag="ACHA44_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19096..19872)
                     /locus_tag="ACHA44_001846"
     CDS             complement(19096..19872)
                     /locus_tag="ACHA44_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8905117.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            19995..>20111
                     /locus_tag="ACHA44_001847"
     CDS             19995..>20111
                     /locus_tag="ACHA44_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HFM8905118.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARV"
BASE COUNT         6687 a         3609 c         4316 g         5499 t
ORIGIN      
        1 aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa
       61 agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat
      121 tgcacagacg taatcagaat gatttcgtcg gctaggaaac gagaggttgc gacacgcccg
      181 gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa
      241 ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat
      301 gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga
      361 gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt
      421 gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt
      481 atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca
      541 tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc
      601 atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt
      661 gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca
      721 atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta
      781 tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt
      841 cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat
      901 gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa
      961 ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat
     1021 cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt
     1081 gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat
     1141 gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt
     1201 actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg
     1261 ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa
     1321 gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt
     1381 gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga
     1441 cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca
     1501 caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt
     1561 cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac
     1621 aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa
     1681 gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac
     1741 tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg
     1801 agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa
     1861 attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg
     1921 aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa
     1981 acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga
     2041 acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa
     2101 aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag
     2161 ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa
     2221 aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat
     2281 cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa
     2341 caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt
     2401 gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga
     2461 tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat
     2521 cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat
     2581 caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat
     2641 tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt
     2701 acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat
     2761 cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa
     2821 catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt
     2881 aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa
     2941 agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc
     3001 taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt
     3061 cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga
     3121 tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa
     3181 aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta
     3241 tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg
     3301 tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg
     3361 ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg
     3421 caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta
     3481 aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca
     3541 ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg
     3601 aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc
     3661 ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg
     3721 tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt
     3781 gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc
     3841 ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta
     3901 tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa
     3961 ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac
     4021 aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat
     4081 gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc
     4141 gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa
     4201 gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact
     4261 gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca
     4321 tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa
     4381 aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg
     4441 aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat
     4501 acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta
     4561 tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca
     4621 aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag
     4681 gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag
     4741 aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg
     4801 taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc
     4861 actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc
     4921 caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt
     4981 gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag
     5041 cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa
     5101 atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa
     5161 cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc
     5221 gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg
     5281 gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat
     5341 gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct
     5401 gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa
     5461 actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt
     5521 gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt
     5581 acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa
     5641 agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc
     5701 atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg
     5761 tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac
     5821 aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa
     5881 ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt
     5941 gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta
     6001 gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc
     6061 ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta
     6121 aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct
     6181 gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta
     6241 tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa
     6301 ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca
     6361 attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt
     6421 tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt
     6481 cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt agattatgat
     6541 ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa
     6601 gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg
     6661 aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca
     6721 caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat
     6781 ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa
     6841 gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag
     6901 atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt
     6961 tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt
     7021 tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa
     7081 aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt
     7141 tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa
     7201 tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg
     7261 gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg
     7321 agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga
     7381 aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc
     7441 aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg
     7501 aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa
     7561 ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca
     7621 aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta
     7681 actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt
     7741 ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt
     7801 attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt
     7861 agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa
     7921 aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa
     7981 atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa
     8041 aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc
     8101 acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc
     8161 gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac
     8221 agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa
     8281 agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga
     8341 agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat
     8401 atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa
     8461 gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac
     8521 ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca
     8581 attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac
     8641 gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct
     8701 ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc
     8761 acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt
     8821 ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa
     8881 ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta
     8941 ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta
     9001 acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc
     9061 acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt
     9121 gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac
     9181 cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga
     9241 caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg
     9301 ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt
     9361 aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt
     9421 gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta
     9481 aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa
     9541 gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag
     9601 taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag
     9661 gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc
     9721 catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct
     9781 caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca
     9841 tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc
     9901 aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg
     9961 gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa
    10021 cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca
    10081 cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat
    10141 tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca
    10201 tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat
    10261 tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag
    10321 ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag
    10381 ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta
    10441 gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac
    10501 ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc
    10561 aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg
    10621 tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag
    10681 ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg
    10741 atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt
    10801 tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg
    10861 agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg
    10921 aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa
    10981 aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg
    11041 atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta
    11101 gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag
    11161 aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca
    11221 atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa
    11281 atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat
    11341 aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc
    11401 tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa
    11461 aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt
    11521 gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat
    11581 tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga
    11641 atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg
    11701 ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact
    11761 gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa
    11821 tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa
    11881 aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc
    11941 atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact
    12001 acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa
    12061 gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa
    12121 cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc
    12181 gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta
    12241 aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg
    12301 aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc
    12361 gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc
    12421 atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag
    12481 gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact
    12541 tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta
    12601 ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa
    12661 atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc
    12721 cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa
    12781 agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat
    12841 tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg
    12901 gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca
    12961 gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag
    13021 ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt
    13081 gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta
    13141 tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc
    13201 tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta
    13261 gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct
    13321 acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg
    13381 tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga
    13441 cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca
    13501 gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt
    13561 ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa
    13621 ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg
    13681 aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa
    13741 gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt
    13801 ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg
    13861 atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac
    13921 ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg
    13981 agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca
    14041 actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc
    14101 tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa
    14161 gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc
    14221 attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa
    14281 ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt
    14341 gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag
    14401 tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata
    14461 tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat
    14521 gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt
    14581 ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat
    14641 tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa
    14701 agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc
    14761 aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt
    14821 gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg
    14881 aatggttttt caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc
    14941 ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc
    15001 acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg
    15061 gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt
    15121 agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa
    15181 gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc
    15241 agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc
    15301 aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc
    15361 tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac
    15421 agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat
    15481 cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa
    15541 aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat
    15601 ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag
    15661 ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag
    15721 tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa
    15781 aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt
    15841 gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt
    15901 agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca
    15961 acggcaggac ttgatcccca gggtagaaaa gaaatgatgg agatgttttg gcgtcttcat
    16021 aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt
    16081 gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag
    16141 gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag
    16201 tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa
    16261 gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat
    16321 atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt
    16381 attagccagt ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct
    16441 tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt
    16501 tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt
    16561 gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg
    16621 tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct
    16681 actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc
    16741 attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg
    16801 ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg
    16861 tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat
    16921 tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag
    16981 aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga
    17041 cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt tgagaggata
    17101 aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgcg
    17161 agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct
    17221 tttatctgaa aagaaagaac gaaaaaattt tataaaaaaa tcattattcc agataacctt
    17281 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgca tacgacggaa
    17341 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
    17401 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
    17461 atgccggagt acatgcgatt gggcaagtaa tccattttga ttatccagag gagcgtccgt
    17521 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
    17581 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
    17641 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
    17701 cttaccctct tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
    17761 atttcacctc tttttgtgca tcaggaagct cgatagagga taaagtacgt acgatctacg
    17821 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
    17881 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
    17941 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
    18001 ctacagcaca tcctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttgtga
    18061 cagaagtggt cagcttcaag aaataagaag gaatttacga aaattacttc tcaaaatttt
    18121 gcaaaatttc ggcttatttt cgaaggagct acttctggaa caccgtgaat cagaggttgt
    18181 gaaaaagctg ccctgcatca agtaataaga acaaccaaac aaaaatagct cgtcatattt
    18241 ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt aaggaagagg
    18301 ttgtgaaatc agcgttttga cctgagtatt agagaaacaa acggaaaaat agttcctcat
    18361 attttgccgt ttgttgagct aataccgaag gtcagctgat tgaacaccgt ttaagaaaga
    18421 ggtcgtgagc ctgtcattta actctgagtc acaagtaaca atttcaaaaa acagcttctc
    18481 atctttgatg aatatcgtga cttgtggacg aggcgttgat aagtgaatca tctttttgtt
    18541 acctattttt tctgtaacca aaaaacgatg gaacgttgtt ttgattgccc ttaatgagga
    18601 cagtcggaac aggtttgtca tttttatatt taaatggcac aaatttttct aattatttgt
    18661 catttagcgc tgaaaatttt tgctatttat ctaaaaaagt gataactact taagatgaag
    18721 cagaatattt acgccatacg gcatacaacc gcgaaaaata taaaaaaaag aaaacgattg
    18781 aacgcgtttt tgcagtcgca aaggaaaagc atggaatgcg ctacatcaag tatcgagact
    18841 tggaaaacaa tcaggtgtat acgctgattg tttttgctgc aatgaatctg aaaaagttgt
    18901 ccacttggca gttggaacat atgtcttcat atcttttatt atacaaaaat agcaaaagca
    18961 ccagtctaca ctcttagtaa agaggaaact gatccttttg tctacattct taaagtactt
    19021 aaactaagta ctttatttta ctctttagga ttgatacagt agcatctatc cctttattaa
    19081 taatctttta tttttttaaa taaagatgtt gtctccatct tcaggcacta aaactttttc
    19141 ggaaattcct ttttctttat tgaaagactt taattcttct cttgacagtc cccaatggtt
    19201 tacagcttcc atatgaacgg caataattgt ggaatctggt attgctttat gcaattcata
    19261 taaatcgtct ttccccataa taagagattc tccaactaaa aaatgattat ctccagcatt
    19321 tgctactatg atttctggat tatactgatc aatagtttgt ttaacagctt cataccaaac
    19381 tgtatctcct gtaaagtata gtgtcttctc agtcgaatgt ttgaaaacaa taccgcaaac
    19441 attacctaca taattcaaga tttctcctct gccatgtttg ccttttgttt taaccaattg
    19501 tatagattca aaaactgtgt cttctcgtag tacctccaca tttttaaaac catatagtcg
    19561 aatttcattc gcatcatttt catcttgcac aaataatttt atttctttag gtaggacctc
    19621 tttcgctata tcgtcccaat gatctagatg taaatgtgtt acaataacag catcaatacc
    19681 gttgataata tcttcgactg gcattggcaa atcaactaga ggattcattt gatcttttct
    19741 tggtgatgga aaaggacctt tcgaaataaa tggtggaagt gttccttttt ccgctaacat
    19801 tggatcaatt aaaaattttt tacctccata gtctaagata gctgttgcat tacgaatgtg
    19861 atgtatattc ataaacgttg tctccttttt gttgatatga aatattttta gtataattca
    19921 attccataca ataaataaac cgaacaaaaa aagtctttta ctatttttac ttaatttatg
    19981 aaaggttgtt taccatgtta gaccaaacag atataaatat cattgaggaa ctatcaaaaa
    20041 atagtcgaat cacaatgaaa gaacttggag aaaaagtaca tttgactgga cccgctgtct
    20101 cggcgagagt g
//
LOCUS       DAYYGS010000055        19635 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000055 DAYYGS010000000
VERSION     DAYYGS010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.055"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA44_001848"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="ACHA44_001848"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFM8905119.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="ACHA44_001849"
     CDS             complement(1174..1869)
                     /locus_tag="ACHA44_001849"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HFM8905120.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="ACHA44_001850"
     CDS             complement(1882..2598)
                     /locus_tag="ACHA44_001850"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFM8905121.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3536
                     /locus_tag="ACHA44_001851"
     CDS             2928..3536
                     /locus_tag="ACHA44_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFM8905122.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3861..4184
                     /locus_tag="ACHA44_001852"
     CDS             3861..4184
                     /locus_tag="ACHA44_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8905123.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4366..5736
                     /locus_tag="ACHA44_001853"
     CDS             4366..5736
                     /locus_tag="ACHA44_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HFM8905124.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5866..7167)
                     /locus_tag="ACHA44_001854"
     CDS             complement(5866..7167)
                     /locus_tag="ACHA44_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFM8905125.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7521..9512)
                     /locus_tag="ACHA44_001855"
     CDS             complement(7521..9512)
                     /locus_tag="ACHA44_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HFM8905126.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9656..9922)
                     /locus_tag="ACHA44_001856"
     CDS             complement(9656..9922)
                     /locus_tag="ACHA44_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8905127.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA44_001857"
     CDS             complement(10432..12558)
                     /gene="pnp"
                     /locus_tag="ACHA44_001857"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFM8905128.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA44_001858"
     CDS             complement(12746..13015)
                     /gene="rpsO"
                     /locus_tag="ACHA44_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFM8905129.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13266..13829
                     /gene="def"
                     /locus_tag="ACHA44_001859"
     CDS             13266..13829
                     /gene="def"
                     /locus_tag="ACHA44_001859"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFM8905130.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14082..14603)
                     /locus_tag="ACHA44_001860"
     CDS             complement(14082..14603)
                     /locus_tag="ACHA44_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HFM8905131.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14606..15448)
                     /locus_tag="ACHA44_001861"
     CDS             complement(14606..15448)
                     /locus_tag="ACHA44_001861"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFM8905132.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15441..16166)
                     /locus_tag="ACHA44_001862"
     CDS             complement(15441..16166)
                     /locus_tag="ACHA44_001862"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HFM8905133.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16342..16638
                     /locus_tag="ACHA44_001863"
     CDS             16342..16638
                     /locus_tag="ACHA44_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905134.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     regulatory      16894..17068
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17206..18798
                     /locus_tag="ACHA44_001864"
     CDS             17206..18798
                     /locus_tag="ACHA44_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFM8905135.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18811..19551
                     /locus_tag="ACHA44_001865"
     CDS             18811..19551
                     /locus_tag="ACHA44_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8905136.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5620 a         4028 c         3725 g         6262 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcaaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgcgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aagaaagcac aggtgaaggt ggcgaaccag aagtgatcgg ttcaacagaa gaataatact
     3541 gaaacaatca ggtggtgtta gaagcggcgg gtcagcagct tttaacacgc cttttttatt
     3601 tctaatcgtt tattttcata tggggcttcc acttttgtaa atggctttac aaacgaaaaa
     3661 tcctaattta ccaagctttt cctcgcatac ttttatcgta cctttataaa aatttatact
     3721 ttgaaaaaaa gtattcaatt attgttgcat ttttcctgat tctttattag aatgaaccct
     3781 cgttgaagta agtatttcta gtttatccta ggattaatta taaaaataaa acattcgttg
     3841 attcgaagga ggttaaacag atgtttgaaa gttttgatag cagccgcaac cgttacgctt
     3901 cagttggtgt cgtatcaagt ttgccagatg aattgattga cagtatatgg tttattattg
     3961 acctagatct taagggagtc attccattag aaaatatatt aacctttgat ttgatcgaaa
     4021 atcgtggaaa agtgacgatg catttttctc aagcaggaag caatgtggaa atggatattg
     4081 atttgccgtt tgcctattcg aaccaatttc ctgctcaagt atacgcctat gatgacggta
     4141 cgagagaaac gatcctttta ccaagtgaga ttagacaata ttaaggtgat agataaacgt
     4201 ggatgtccct tattttacag accaggccaa gttgttgatt ctttgactca attcttggct
     4261 tctttttatg gagtttcata aattatcatt gatttctaat tggtattctc aggataaacg
     4321 ctatatacta ctaactaaat tttaaagata taaggagttt aaaagatgcg cgatttaaca
     4381 aaaggaagcc ccgctaaact gattttgatg ttcactgttc ctttactgat cggaaatgtt
     4441 tttcagcagt tctacaatat gatcgatatg atcatcgtgg gtcagacgct aggtaaaaat
     4501 gcgctggcag cggttggtgc aactggaagc ctgacatttt tgatcattgg ctttgcccaa
     4561 ggtttaacag ctggtttagc cattattacc gcacaacgtt acggtgcaaa agattatcgg
     4621 ggtctgaaga aaagttttgc tgctagtgtt gtcattagtc ttgtcgtcac tgtgatttta
     4681 actgtcctaa gtctcctgtt tattcgtccg atgcttcaat tgatgcaaac tcctcctgaa
     4741 atacttgatc aagcacaaac atttatttca attattttat tagggatctt tgcatcagtc
     4801 agtttcaatt tgctttctaa tgtcattcgc gcacttggtg acagccgcac accactattc
     4861 tttctgatca ttgcggtcat catcaacgtc gtacttgatt tgatctttat tatctatttt
     4921 ggcatgggtg tggaaggtgc agctattgct acagtaatcg cacaagtttc ttccagcgta
     4981 ctgtgtctag tgtatatcaa gaaaaaaatg cctttgttac agttacgcaa aaaagatttc
     5041 aaatttgaaa aagaagagtt tgctgtacac ctaaatgctg ctttgccaat ggcgtttcaa
     5101 tcttcgatca tcgctgttgg tgctatcgtg ttacaagcag cactaaatag tctagggaca
     5161 gatgtcgtag ctgctcaagc cgctgctggt cgtatcgacc aatttgcaaa tcagccgatg
     5221 atgtcatttg gtattgcaat ggcaactttc actgcacaaa actatggtgc gaaagaatat
     5281 ggccgaatct tgcaaggggt acgtcaaact ttactgatga gtgtcggatt tagtctctta
     5341 gcaggtgcta tcgtcatttt cttcggtcat ttctttgttc aattgtttgt tagcccatca
     5401 gaaacacgtg tatttgaatt agcacaaacc tacttcaata tcaatgggtc tctttactgg
     5461 atcttagcag tcctgttcat tttacgctac acacttcaag ggcttggaca aagcaagatc
     5521 cctacgatcg ccggtatgat ggaattgctt atgcgttctt ttgcagctat cgttcttaca
     5581 ggaatgtggg gatacccagg agcagcagca gcttctcctt tagcttggat cggttctgtt
     5641 gccgtgttgc tttactctta tatgcgttcg atgaaacaat tgaaacacat gggtgaagag
     5701 gaacatttca cattagaaca tgcagaagca agatagcctt gattaaaaga aatcgttgcg
     5761 gctaaaaatg aaaactttta ttgaaaatat cttcttcgtt taccgaaaac aggtttttta
     5821 gataacaaaa aggctgtgac aaaaaatgtc acagcctttt ttcttttatt ctgtttttct
     5881 ttccgtggac tctactttga aataatcgat atgtgcatgc tggtattcta ataccgtgaa
     5941 acggaatgat gcaatctcaa tgattgttcc tgcttttatt tctttatatc ggctagaagc
     6001 aaagcccgca agtgtagtaa aattcgtttc ttcaaactct ggaatctcta catgaaaata
     6061 acgctggaaa tcatctaatg ttagtttgcc ggagatgata taagagccat ctgactgttc
     6121 cgctatcaat gcttcgtcca aatctgtctc atctcggatt tccccgacga tttcttctaa
     6181 aatatcttca atcgtcacta agccgcttgt ccctccgtat tcatctttta agatagcaat
     6241 atgcttaccc ttttttcgca tgatcgtcaa taattttttt atagggatcg tttcagttgt
     6301 tacgatcggc tcttcaatca actgttctac tgtcagctgg tcatctttat aagattgctt
     6361 gatcaaatct ggcaaggtga cataacctaa aacattgtct tttgattcta agataaccgg
     6421 atagcgagta tgtccttgtt caatcgaagc ttttatcgct tctttgacgg tttctgaagc
     6481 ttcgaatact tgcatggacg tacggtctac ttgtatatct tttgccaagg tatcatcaaa
     6541 ttcaaacacg ttttccaaat aataatattc ttctttggtc aactctcctt tttcataaga
     6601 attcaatgcg actaatgtca gctcttcttg agacaatgtc tcttctcctt cagatacgag
     6661 acgcacacct aatagcttcc ctagccctgc agctgaatga ttcaataccc aaataaaagg
     6721 aaacattact ttataaaaat aatgaagtgg tttaactacg aacaagacga ccttctccgt
     6781 attgacgata ctatagctct taggaagcag ttcgccaata accacatcga tatacgtaac
     6841 taaaataaat cccagcgcca cagagattgt actgctaata gccgaactaa aaggtagttt
     6901 accgatcact ggttctagta aagccgcgac agatccttca ccgacccacc cgatgatcaa
     6961 accggttagc gtaattccta attgactagc tgataaatag ttatctaaat ggtgtgtaac
     7021 atgaagagct agcttggcgt tctttacatt ttccttttct agttgttcca atcttgatcg
     7081 tctgattttc actaaagcaa attcacttgc tacaaaaaat gaagtaattg caatcatcaa
     7141 aaataaaata atgaaattga taaccatatt ctcccgcctt tctctttttc tattgtaccc
     7201 tttttctaca aaaagattga aaataatgcg tattcatatt taggaataaa aaaggagatg
     7261 cggcattttt cagccacatc tccttatcac ttcaaaatca ttgcgttact tgttgctaaa
     7321 cagctttttc atgacctctg ataaaaacgg tattcaaaag cttgcatcta cggcattaag
     7381 cccaaacaga tgaaaaacat gagaagctgt ttttatctgc tcgttcttac tgcttgttgc
     7441 agtacagctt tttcatgacc tctgataaac ggtgttcaag tagctgacat tcgacattag
     7501 ttcaggtcaa agcgctactt tcacaaccgc tctttcaacc atgttttccg agtatttcca
     7561 atgataagcg gaacagtttc atgttttact tcgctgtact ctagcccaaa ccatgtttct
     7621 ggcgaggtag cgattttttt gattgccagt tttgcctgca tgatttgacg ttgccatttt
     7681 cctagagttg tcacattgga aactacttca tcaatcagga caaattggaa atcgccaact
     7741 tctcgccctg gtgtcagaga gtatctttgt ggctgttttg gcaaacggcc ttgtttcatc
     7801 aaatcatgga tgacttggcg aacatatacg ttgatttcct gttggaccct gaaacctaag
     7861 aacagattca cttggacaat aaaatcagtg tccatcatat ctacttgata ttctttcgta
     7921 tatggctcat ccgtcacttc cacatgaaca aaccaataga ctcgtgctct tttaggccgt
     7981 ttatctaaaa tcgaatagat gaactgtcgg ccaactttat cttcagccat atcaggaacc
     8041 aagaagacca catttgtctg atagattggt aaagaagtat catcacgtaa ttctctaagc
     8101 tgcgggatat aatctctcaa agacacatcc tcggttgacg attcgcgaat aatgttccct
     8161 tgttcccaaa taaacatcac gactaagatg atcaaaccaa tcagaacggc tacaaaaccg
     8221 cctcggaaga atttgacaac acttgagata aagaaaacga cttctatcgc accgaagaaa
     8281 aaggtaacta aatatgcaag ccaacgagaa tgcccatttt ggataagata aaagtaaagc
     8341 agtgctgttg tcatcaacat cgtcacagtg atcgatagac cgtaagcagc ttccatatga
     8401 tgagacgttc ggaatgtgac aaccactaga gaacaggcga tccataaaat cagattgact
     8461 gctgggatat acatttgccc gatactgttc ccaggataca tgatctttaa ccgaggcagc
     8521 agtttcaact tgattgcttc tgaaactagc gtaaacgaac ctgtaatcaa tgcttgcgaa
     8581 gcaatgactg cagcgactgt tgcaaaagca acgccgatca cgatccaact ttgaggcatc
     8641 atttggaaga atggattcag gttcttgatt tgctgtgctt cagggttttg ataaacattc
     8701 aacaaccacg cagcctgccc aaaataattg ataaccaaac aaattttgac gtaaggccag
     8761 ctcagacgaa tatttcgttt gccgacatgt ccaagatcgg aataaagcgc ctctgcacct
     8821 gtcgtagcca ggaaaatatt tccaagaatg aacaagccta atttgttatc tgcactaaaa
     8881 agcagctgga tagcataata aggattcagt gcacggataa ctgtccagtc ttgactgaag
     8941 ttcattaatc ccattacgcc taaaaaagta aaccaagcga acatgattgg tccgaatgct
     9001 tttccgacaa tatctgtacc aaagcgttgg accatgaata aaacaaaaat aataactaaa
     9061 gtgataatga cgatcacgtt ctggttatta ccgaaccggt caaaaaactg cggaattcct
     9121 cgcaagcctt caatcgcagt cgttaccgtc acagcagggg tcaatacccc atctgctagt
     9181 aaggcagctc ctccgatcat tgcaggaatg atcaaatatt tcccaccttt tcggacaagt
     9241 gtgtagagtg agaaaattcc accttcacca tggttatctg cattcaatgc aatcacgaca
     9301 tatttgattg ttgtcagtag agtcaatgtc caaaaaatca atgagactga tccgataaca
     9361 aaatcaggat tgattcctga caatccgcca ttatcttcaa caatcgattt cataacgtaa
     9421 agcggacttg tcccgatgtc tccgtagacg actcccatcg ctacaagcag gccagccatt
     9481 gatatctttt tggtattgtg cgttgttgcc acgtttttcc ctcatttctt gttatatgga
     9541 tatcctttag gtaagcttga atgatagatg atattcttat ccatgacacc cgctttccta
     9601 aattgcttct aattatccaa gtaacacact gaagtgtcaa cttatttgaa taaaattatt
     9661 ctttctttgg acgttctctg atacgtcttg ccacaaatga ataaagagca aggcaaaaaa
     9721 ttgcacccaa taatgctggt atcaaataaa aaccgccgag ttccggtccc agtggtccta
     9781 gtaaaaattc gccgagccaa ctacctaaaa aaccgataat gatatttcca gatacgcctc
     9841 ccgggacttc ctttcctaaa attgcgccag ctatcaaacc taatataccg ccgataaata
     9901 acgaccataa aaaattcagc atctcctttc cctcctttat ccatatttct cacttttatt
     9961 ttacgcttta ttcagaagga ctccaaaaat atgcgccaag aaactccaga aaaaaagaag
    10021 ttgtaacaaa agtgttcagc ttatttccgt agaagctgct tctggaacac cgtgaatcag
    10081 aggttgtgaa atcagcggtt tgacctgagt attagagaaa caaacggaaa aatagttcct
    10141 catattttgc cgttttttga gctaatatcg agggacagct gattgaacat cgtttattgg
    10201 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa cagccaagta aaaacagctc
    10261 ctaatgtttt taacttggct ggacttatta ccgcagatgc aagcttttga acaccgttta
    10321 ttcgaaaaaa aaggactgtg acacggcttt tgtcacagtc cccttttttc gaataattat
    10381 tgtccccctg tcaccttcac gatcacaagt acattattcc tatgaattaa attatttttc
    10441 ttcatttttc gttgaaccat cttcgttcaa cattgctttt cttgacagat ttacgcgtcc
    10501 ttgtttatcg atttcagtta ctttcacaag gatctcatca cctagtttaa caacatcttc
    10561 gactttattg acacgatcgt ttgctaattg ggaaatatgg acaagtccat cttttccttt
    10621 gatcaagttg acgaatgcac cgaatttttc gatacggaca actttcccta gataaacttc
    10681 accaactttg acttctttgg tcaaatcttc gatgatcttg atagcttttt tgatcatttc
    10741 tgcatcagct gaagcaatac ttacttttcc atcttgatcg atatcgattt tcacaccagt
    10801 ttcatcgatg attccgttga tcgtatcgcc gcctttaccg ataacatctt tgattttagc
    10861 aggttcgatc tggatcattt cgatttttgg tgcatattgg cttaattctt cacgtggagc
    10921 agcaatcgtt gaagtcaatt cttctaagat ttccatacgt gcttgttttg cttgagttaa
    10981 tgcttcagtt aggatttgtt ctgtgattcc ttgaatcttg atatccatct gtaaagcagt
    11041 gataccatct tttgtaccgg caactttaaa gtccatatcg ccaagatgat cttccagccc
    11101 ttggatatca gttaggatcg tatagttttc accatcagaa acaagtccca tcgcaatacc
    11161 ggcgactggc gctttgattg gtacacctgc atccatcaat gctaatgtgc cagcacagat
    11221 acttgcttgg gaagaagaac catttgattc caatacttct gctactaaac ggatcgtata
    11281 aggaaattct tcttctgaag ggattacttg tgctaacgca cgttcaccta atgcgccatg
    11341 tccgatttcc cgacgccctg gagagcctgc acgtcctgta gaaccaacag aaaattgagg
    11401 gaaattgtag tggtggatga atcgtttgct gacttccacc cctaaaccat caatgatttg
    11461 gtgctctcct agaggtgcga gtgtacaagc tgataaagct tgtgtttgtc ctcgtgtgaa
    11521 caaacctgat ccatgtacac gtggcaataa gctgacctct gatgacaatg gacggatttc
    11581 atccaattta cgcccgtcag gacggatctt atcgatggtg atcaattcac gtacgacatc
    11641 tttttcaaga tcttctgtga tttgtttaac ttctttcaac aagtgatctt catcttcatg
    11701 acctgcaaat ttctcagcat aagcttcgcg gatatcgatc ttcacctgct cgatattatc
    11761 ttcgcgagca agtttttctt cagtcattac tgctgctttc attgtagcgt aagaagcatc
    11821 aaagatttct tttttcaata cttcatcaac ttgcagtaaa gtcacttcca ttttttcttt
    11881 ccctactgct tgaacgattt cttcttggaa agcaactaac tctttgatag cgtcaaaacc
    11941 aaacagcaat gcaccaagca tgtcttcttc tgaaacttct ttcgcgccgc tttcaaccat
    12001 attgatggct tgtttcgttc cggcaacagt caattcgata tctgtttgtt ctgcttgttc
    12061 tactgttgga ttcaagacgt actcgccatt tacacgacca acttctacgc ctgcgattgg
    12121 gccgtcaaat gggatatctg agattgatag tgctaaagaa gaacctagca ttgctgccat
    12181 agctggcgag cagtctgttt ctacactcat cacgatgttc gttacttgga cttcattacg
    12241 gaagccttct gcaaacatcg ggcgaattgg acggtcgatc aaacgggcag tcaatgttgc
    12301 ttccgtactt ggacggcctt ctcgtttgat gaaacctcca gggattttcc ctgctgcata
    12361 cattttttct tcataattga tcgttaatgg gaaaaagtct gtatcttttg cttctttaga
    12421 ggccacagct gcacttaaga ctactgtatc tccataacgg actaaaacgg ctccgtttgc
    12481 ttgttttgct agctgaccga cttcgatttg taagggacgg ccgccccatg tcgttttgaa
    12541 tacttgtttt tctgacataa cttctccttt tccttctcgt gaaagcaaga cgctactaaa
    12601 caaatgaaaa ctgccgaaag ccgtcttttg agcagctttc atttggttag taggagtcca
    12661 tctagcttga tcacggaatt ttacttaatt gatatacgag taaaaaaagt gagattcatg
    12721 tgaatcccac tctttttgac taaaattaac gacgcaagcc taaacgtttg atcaattcgc
    12781 ggtaacgttg aacgtcagtt ttacgtaagt aagctaacaa gttacggcgg tgaccaactt
    12841 ttttcattag gccgcggtat gaatgatggt cttttttgtg cacgcgtgca tgttcattca
    12901 agtggttgat ttcttcagtt aatacagcga tttgtacttc tggagaacca gtatcccctt
    12961 catggcgagc gtattcgttg atgatttcat tcttgcgttc ttttgaaatt gccatttctt
    13021 tcacctcttt cgattattcc cctgcgactg agtaaaacgt tggtgattcg cttaaccaag
    13081 tggcaggtca tgtgcctaga gcacacagct atactttacc tgaaaccggg ttgaaaatca
    13141 agtagttaat cattaagaaa aggtagtttt caaacaatct tactactact tttccatttt
    13201 tgtggtaaac tataatgcga aaatagactt gaaacacagt cttttaagaa aggggtacat
    13261 attccatgat tacaatggat gatatcattc gtgaaggaaa tccgacctta cgcgaagttg
    13321 caaaagaagt ttctcttcca ttaagcgaag aagatatctc tttaggaaaa gagatgcttg
    13381 agttcttaaa aaacagccaa gatccaatca aagctgaaga actacactta cgaggcggtg
    13441 ttggcttagc tgctcctcaa ttagatattt ctaaaagaat cattgctgtt cacgtaccaa
    13501 gtcctgaccc agaggcagat ggaccttctc taagtactgt tatgtataat cctaaaattt
    13561 tgagccattc tgttcaagat gcttgtttag gggaaggtga aggctgttta tctgttgatc
    13621 gagaagtacc aggatatgtt gttcgccatg ccaaaatcac tgtgtcttat tatgatatga
    13681 atggtgaaaa acacaagatt cgtttgaaga attatgaatc gatcgtcgtt caacatgaga
    13741 tcgaccatat caacggtgtg atgttctatg accacatcaa cgatcaaaat ccatttgcac
    13801 taaaagaagg cgtattggtg atcgagtaga gaattggcag gttccttcga tactatgcta
    13861 caagaaagct ttataaaaaa ggggagtgct caaaagctgc acttcccttt tttatgtttc
    13921 tccactaatc gaaatttaga tagacatcta gaaatcgcaa ttccaaaaaa ttctgtgtat
    13981 tggatacgca tcttttctta ttttttctta tttcttccat atgggtgttt atctcagaga
    14041 tgatagaata gtttctagga taaaaagtag cagctacgat gttatccttc gtagaggatt
    14101 cctgcttcag ctagtttttg tttgatgttt ttcagcatat gcttttctgg gtagcggatc
    14161 gtatgcagat ggatgccgcc tgttaattcg gataacaaag tagcattact tttttgataa
    14221 cgtctcataa aggaatcgat atcttgtggc gttttgatgt gcaaagttcc tgctaactca
    14281 ccgtaaaggg gatgttccac aattacatca atgatttcac caccattttc aacgattagt
    14341 ctcagttctt cttctgtttg atcttttcca tgctggacag cgattttact gatgtgccca
    14401 gtattggttt cttctgtttc gaggatataa cctcttgctg ttgccacgat acttttacct
    14461 gcagctcgta gtaaagcaat atcgcctaca atgatctgac ggctgactcc aaaagttttc
    14521 gcaaacttgc tagcactcac tggtttttct gtatttgcca aggtttgcat gatttgtttt
    14581 cgccgtgtct ctccttcgat cacacttatt cctccttcat tttgttttgg tattcgcgcg
    14641 agtgtttcat caaatcaaca tacgtgtctg ttattgtttc cttatatccc ttatttttca
    14701 gcagactaac aactttatcc aatacttgct gctctctttt tgcatcataa actgcttggt
    14761 tttcttcttt tttcacttgt cctattttag ttaccgtatc cattcttttt tctaataaag
    14821 caaccaactc tcgatcgatc atatcgatct tatgacgtaa tgtttcaaga gactcttctg
    14881 ttagttcttc ctctactgtc ttgatgatac gagcaggatt tcctgcaatc actacattgt
    14941 cgggaaaaga tttggtcaca accgaacctg cgccgactac tacgttgtcc cctagagtga
    15001 cacccggcgt gacgataact cctccgccta accaaacatt attaccgatt ttaatgggtt
    15061 ttgcgtactc cagaccgctg ttacgtttca ctggatgtag tggatgcgtc gccgtgtaaa
    15121 gctggacatt cggagcgaac atgcagttat caccgatttc aatcgtgctg acatctaaaa
    15181 atgtacaatt gaaatttgca taaaaattct ccccaacaaa tatattatat ccgtaatcaa
    15241 aattgataac tggctccata tagatttttt tccctgtacg gccaaaagtc tctttcaaca
    15301 attgggatcg cagttccgct gattctgctt ggttgatgat ttggctttga cttcttgcaa
    15361 acatacggtc ggcaaccagc tcaggatcat tggcaaaata aagctcgcct gcaatcattt
    15421 tttctttttc ggttttgaat tcagtcataa tctgctccta attcttgtat ttttttgaaa
    15481 acaaccgctc cgtcttcttg aataaacgta taaggctccg ctaaataaac aggcgtttga
    15541 ttgattacta agattttggc tgacggctgg cgaaactgta ccagatcaca aaacggatga
    15601 acttgaaaac tcgttccaac aatgacgatc agctctgctt ggctgacggc ataagccgcc
    15661 tgctccatga ctccatcttg aaacccttca ccatataaca cgatgtctgg gcgtatttgt
    15721 cctccgcatg cttgatgttt gtcgctgtga agatattctt tccagtctac cgtttgttga
    15781 cattttcgac aatgacattg atacagactc ccatgaaaat tgacgagttg cgggctttta
    15841 gcctttgcgt gcaaaccgtc gatgttttgg gaaacaaccc aaacattttt ttctttcgct
    15901 agatgtgcta tttccaaatg aatgatattg ggttttgctt caggatgata aatctttttg
    15961 acaaattgat agaatttatc cggttcattc accattgctt gatgactcag caagtattcc
    16021 ggctgctcca tcccgtggta gattcctgac aaagatcgat agtctggcac tccagaaggt
    16081 gtggataccc ctgcaccggt caaaaatgta atttgctgat aagctaatat ctcttttgct
    16141 gcttcgtcta ctgttaattg tttcatgttt tgtcacctca ctttccatta tagcccatac
    16201 tcgaacaaat ttattgttga aaagtcagaa aaatgggaat aaaaaagtgt tatgggcatt
    16261 tgagcaattt atcacaatgt tcactgacaa aagaatgaaa acgtgttaca atactgctgt
    16321 ataaaagtga ggtgaatcga tatgttacca ttctttattt attggggcgt tattctggct
    16381 tgtatcgcct ggttagcgtt aagcgtttat ttctctgttt tttatcttgt acgcaaagaa
    16441 aatggaaatc tttgggcttt tgctttattc aacgtgattg ccgcaattgt tttagccatt
    16501 acattagccg tatatcgaac atggggctgg ggaatcaccc aatactcaag tttgatttat
    16561 ctaatattag ccatttatgg cgtcgttgtt cttttgcaag cgatcctagg acgtgagcca
    16621 aagaaagcgg cagcttaaat gatcgagatg gaactggaca caggaaattt tctgttctag
    16681 ttcttttttt attgtattac atacattttt gtgtataatt atactatcgt ttgcaatgga
    16741 tagatgattc acattttgat tgttttttcg gtaaaagtaa agatttctat atttatcagt
    16801 attttttcaa aaaccagcag ttatagattg acaaagcctc tcgagtatcg taaacttttt
    16861 ttgtataaat aactgaatac gagatgcctc aatcaatgag gtagaggtcg cgagaatcat
    16921 taaccctgtg gagtgaagca aacatggaga agcaggaata gggaggatcg ccgaaatgtg
    16981 cacttttttg cttggagtgt atgttgggac gcaagttaag agcttgcgga ctgtcttagc
    17041 agtgatgcta agttgcgcta tgttttgtga gaggtttatc accttatgga attttagcgg
    17101 cctttttata tctataaatc ggtcgctttt tattttttca gataaagctt taccgcattt
    17161 catctagacc gctcgtgtat catataatca gaaagggaga aaaaaatgaa aaaaaatatg
    17221 gtttcagttg ccgtactatt tgcttttgtc ttctcactat tcagtgtggg atctctggca
    17281 aatgcagatg aaaaaacacc gagcgatgag tttcgcgtag gtatggaggc tgggtatgca
    17341 cccttcaact ggtctcaaca aacagatgag aataatgcct tgccaattca aggccaaaat
    17401 agttacgccg gcggttatga tgttcaaatc gctaaaaaag tagcagatgg tttagggaaa
    17461 aaattagtca tcgtccaaac gaaatgggat ggtctggctc ctgcccttca atcaggcaag
    17521 atcgacgcga tcatcgccgg aatgagccct actgctgaac gacgaaaaga aattgatttt
    17581 accgatcctt attacgaatc acaattagta gtagtagttc aaaaagatag caaatatgca
    17641 aaagcaaaaa gtttagcaga tctttctgga gcaaagatca ctgctcagtt aaatacattc
    17701 cactatactg tgatcgatca gataccagat gtgcagaaac aacaagcaat ggataatttc
    17761 tcagcaatga gaacagcttt agcttctgga atgatcgatg gttatgtcag cgaacgtccg
    17821 gaaggtgtaa cggcaactag tgtgaacaaa gacttggaaa tgcttgaatt cagcggtgca
    17881 gatggcttcc agacaaaccc ggaagatgtt cagatttctg ttggtatgcg taaaggcgac
    17941 ccggatatcc aaaaagtaaa ccagattctt tctggcatct ctaacgatga acgaaccaaa
    18001 attatggacc aagcagtcaa agatcagcct gctgcaactg atagtgaaga aaaacctggc
    18061 atcatttctg atttcactac gatttggaaa caatacggca atatgttctt acgaggtgct
    18121 ggacttacct tgttcatcgc attgatcggg acagttgtcg gtactacact tggattactg
    18181 attggtgtct tccgttcggt acctgaatca ggaaatgcac tgactcgctt cttccaaaaa
    18241 atcggaaatg cacttctttc tatctatgtc gaagtattcc gcggaacacc gatgatggta
    18301 caagcaatgg tcatcttcta cggtctggca ctcgcttttg gcatttcatt gaaccgaacg
    18361 attgccgctt tgtttattgt ctctctcaat actggagctt acatgacaga gatcgttcgt
    18421 ggggggattt ttgcagtaga tgaagggcag ttcgaagctg cgcaagccat tggtatgacc
    18481 catggccaaa cgatgagaaa agttgttatt ccacaagttt tacggaatat cttgccagca
    18541 acaggaaatg agtttgttat caatatcaaa gatacagcag ttttaagtgt aatcggcgta
    18601 gctgatttgt tcttccaagg aaactctgct tccggtgcga atttccaatt cttccaaaca
    18661 tttacgatcg tcgggatcat ctacttagtc atgaccttta cgatcaccag aatcctgcgg
    18721 gcggtagaaa agaaaatcga tggtccttca gcttatacca agcttgaaga aatcgacaat
    18781 tcaaatttgc aggaataagg aggaagaatc atgaatgaaa aaattattga aatcaaacat
    18841 ctaagaaaaa gcttcggtga aaatgaagta ttgaaagata tcagcgtaac agtcaataaa
    18901 ggtgaagttg tgacgatcat cggttcttcc ggttctggta aatctacttt cttgcgctgt
    18961 atcaatttac tggaaaaacc aacaggcggc gagatcattt ataatggaga aaatgtatta
    19021 gctcctaaat ataatttacc taaatatcga acgaatctag gtatggtttt ccaatccttc
    19081 aacctgttca acaatatgaa tgtcttggaa aactgtatgt ctggtcaaac aactgtcctg
    19141 aaacgagata aagaaactgc caaaaaagta gcaatcgaaa atctggaaaa agttggtatg
    19201 gatcgttata tcgaagccaa accagcacaa ttatctggtg gacagaaaca gcgtgtcgca
    19261 attgctcggg cattatcaat gaatccagat gtattgctat tcgacgaacc tacttcggcg
    19321 ttagaccctg aaatggttgg cgaagtgctt aatacgatca aagacttagc acatactggc
    19381 ttgacaatgc ttatcgtgac gcatgagatg gaatttgcaa aagatgtatc tgaccgtgtc
    19441 atttttatgg ataaaggtgt gattgcagaa gaaggtacac cagaagatat ctttgtccat
    19501 ccgaaagaag aacgtacaaa agaattcctt tcacgtattt taaatgctta ggatcaatag
    19561 ctgattggga caaagagctg cggtgtttac cgcagttctt ttttatgaat tactcataaa
    19621 aggtaaattt ctgaa
//
LOCUS       DAYYGS010000056        19534 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000056 DAYYGS010000000
VERSION     DAYYGS010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.056"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(133..297)
                     /locus_tag="ACHA44_001866"
     CDS             complement(133..297)
                     /locus_tag="ACHA44_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905137.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            592..1920
                     /locus_tag="ACHA44_001867"
     CDS             592..1920
                     /locus_tag="ACHA44_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HFM8905138.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2122..2955
                     /locus_tag="ACHA44_001868"
     CDS             2122..2955
                     /locus_tag="ACHA44_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFM8905139.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2971..3708
                     /locus_tag="ACHA44_001869"
     CDS             2971..3708
                     /locus_tag="ACHA44_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HFM8905140.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3863..4831)
                     /locus_tag="ACHA44_001870"
     CDS             complement(3863..4831)
                     /locus_tag="ACHA44_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFM8905141.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5063..5896
                     /locus_tag="ACHA44_001871"
     CDS             5063..5896
                     /locus_tag="ACHA44_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFM8905142.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5930..6769
                     /locus_tag="ACHA44_001872"
     CDS             5930..6769
                     /locus_tag="ACHA44_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8905143.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6795..7355
                     /locus_tag="ACHA44_001873"
     CDS             6795..7355
                     /locus_tag="ACHA44_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8905144.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7554..9275
                     /locus_tag="ACHA44_001874"
     CDS             7554..9275
                     /locus_tag="ACHA44_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HFM8905145.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9440..10582
                     /locus_tag="ACHA44_001875"
     CDS             9440..10582
                     /locus_tag="ACHA44_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HFM8905146.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10598..11809
                     /gene="thiI"
                     /locus_tag="ACHA44_001876"
     CDS             10598..11809
                     /gene="thiI"
                     /locus_tag="ACHA44_001876"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFM8905147.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12382..13029
                     /locus_tag="ACHA44_001877"
     CDS             12382..13029
                     /locus_tag="ACHA44_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8905148.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13158..13374
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13422..16067
                     /locus_tag="ACHA44_001878"
     CDS             13422..16067
                     /locus_tag="ACHA44_001878"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFM8905149.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16377..17690
                     /locus_tag="ACHA44_001879"
     CDS             16377..17690
                     /locus_tag="ACHA44_001879"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HFM8905150.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17680..18333
                     /locus_tag="ACHA44_001880"
     CDS             17680..18333
                     /locus_tag="ACHA44_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8905151.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18388..19080
                     /gene="radC"
                     /locus_tag="ACHA44_001881"
     CDS             18388..19080
                     /gene="radC"
                     /locus_tag="ACHA44_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFM8905152.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19230..19430
                     /locus_tag="ACHA44_001882"
     CDS             19230..19430
                     /locus_tag="ACHA44_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8905153.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         6581 a         3295 c         4139 g         5519 t
ORIGIN      
        1 aaagcttatg caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat
       61 gatgaatcaa gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt
      121 ggctactcat aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc
      181 tacaccaatc aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt
      241 attcataagt atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt
      301 attcatctcc tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat
      361 cttattgtat gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc
      421 atagaaatat tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga
      481 agttgtaagt atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa
      541 attcttaaga gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct
      601 attacgcctg atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag
      661 gcattgcagc gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca
      721 catgtggaga atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa
      781 atgcaatcgg ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg
      841 aatcaattcc atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa
      901 tatgaaaagg caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat
      961 agccggaaag tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg
     1021 atcgtttcat tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg
     1081 aatagttcaa attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca
     1141 gtagtgttac ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa
     1201 gaagaaatgc tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa
     1261 gaattcgatt ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca
     1321 ccacaaattt tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc
     1381 aatgcaccaa caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat
     1441 gtcgttggcg ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta
     1501 gcgattgctc agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct
     1561 tctactagaa ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat
     1621 acggatttta caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat
     1681 gcgatggtat tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa
     1741 aatagctggg gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg
     1801 gacgaatata cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat
     1861 gcgttcaatg aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag
     1921 acgagtattt caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct
     1981 ttttatcttt aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa
     2041 ggataaggat tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa
     2101 ttgggagtag gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat
     2161 ggtaaagtga ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa
     2221 catcgtgctt attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa
     2281 gtagacggat taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt
     2341 gaaacaagtg aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa
     2401 cgagatcttg ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt
     2461 cgtaaagata actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg
     2521 acttttttct ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga
     2581 tggaatgtac agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt
     2641 gaaccaaaaa agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc
     2701 tttgaccagc aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa
     2761 caagatattg caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat
     2821 caaactatat ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat
     2881 tatcttttgg cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta
     2941 ctagaaaatt tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa
     3001 gtttcatatt atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta
     3061 gttatcaaaa cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat
     3121 agctttggac ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga
     3181 gtcggtgaag taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat
     3241 cggaacttct ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc
     3301 gtattgatgg attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct
     3361 tttgagagtg agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg
     3421 ggcttcatgt tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat
     3481 aatgcgatgt attttaattg gatcattgac gtattaggat atgacttttt gactacgcat
     3541 gttccagaaa aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa
     3601 agccattttg aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg
     3661 caagaacagc tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt
     3721 gacagaaggg ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt
     3781 ttgtaaattt tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag
     3841 tttagctatt agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca
     3901 aaccatagtt tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac
     3961 agaaaataat ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct
     4021 ctgcgattcc attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct
     4081 tttccaaagc agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca
     4141 aaccgtctac tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg
     4201 ctttaacgat cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa
     4261 gttcctgcaa gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg
     4321 ttcggaaagt agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct
     4381 catcgttcat gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa
     4441 tcgcactttc aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc
     4501 cagtcaaaac aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt
     4561 ctaatgtatc tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca
     4621 agatacgttc ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga
     4681 agatatcttc aactaataat tctacatgct tggggattgt gatttctgca tctaataatg
     4741 ggtttgccac atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc
     4801 cgccggtgtg tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc
     4861 atagccgtta aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac
     4921 gaatagccta acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg
     4981 acctcctaat acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa
     5041 tcaatcacga aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga
     5101 caaattaact ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt
     5161 ggaattagat actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag
     5221 tgaagtacct gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg
     5281 agactggaag ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat
     5341 gtcgccaatg gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt
     5401 caaatgtacg aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta
     5461 tcctgtttct atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac
     5521 aagaaacttt atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct
     5581 gaatacaacg attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt
     5641 ccatggtagg aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac
     5701 gttatatcgt tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga
     5761 ttctaagaca aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga
     5821 aaatgcatta gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca
     5881 aatggatctg ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc
     5941 tatttttttt gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac
     6001 aaaacaagca attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg
     6061 cagcccagta aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta
     6121 taatggtcaa ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa
     6181 agtattggaa catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc
     6241 gagaaatcgg ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg
     6301 actagtcagc tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt
     6361 ttttagccct caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat
     6421 ttatcaatgt atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc
     6481 cgagtgtacg tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc
     6541 aaaattacta ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat
     6601 ggcattcggc gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc
     6661 catggggaat gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga
     6721 agaagatggg atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga
     6781 aggaaaaaat cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa
     6841 cttttgatcc aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac
     6901 gtgctgcgtt caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag
     6961 aacaactgca tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa
     7021 tacttggtaa aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag
     7081 atatccttta ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat
     7141 tcgaaatcgt ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg
     7201 accctgtaac aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac
     7261 ctaaaatcgc ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg
     7321 ctgatatagg catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc
     7381 tttatacgat ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat
     7441 gctataatct agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag
     7501 cagaacgcca tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa
     7561 ataatgttat catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg
     7621 ttagttactt gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg
     7681 aagaattatt tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg
     7741 ttgggcaaag ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt
     7801 cctcgttcgc tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc
     7861 ggttcttaaa aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga
     7921 aacaagtgac ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact
     7981 cactagctgt tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag
     8041 acgagcatga atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa
     8101 aagaatttac gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg
     8161 ttttggataa agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac
     8221 cgctgtatga ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt
     8281 acgagaaatt gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg
     8341 caaaagtaag tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa
     8401 cggtcgaatt tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag
     8461 aacgggaaat ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata
     8521 tcaaacatac aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa
     8581 gctatacatt gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag
     8641 aaatggaacg tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt
     8701 cagaagtgcg cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac
     8761 aagtagagat cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa
     8821 agatcgatac attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt
     8881 tgcctggctt gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag
     8941 aactaagtgt tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg
     9001 ctctatgtga agaagatcta gaattattag ataagaagac acatgattta gtagatgcag
     9061 cagcattaac ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag
     9121 tgagagaagc aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag
     9181 cattagacga aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg
     9241 aagattttta ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa
     9301 gagcaagacg gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa
     9361 gtgaaaattt cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt
     9421 caaaaaagag gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc
     9481 gcaaagctta gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt
     9541 gcatgattta ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga
     9601 tttgatccat gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa
     9661 ctgggtatta aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc
     9721 aggagtcgaa catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga
     9781 agtaagtatc gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt
     9841 aataagaaaa gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat
     9901 tcagcccatc caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt
     9961 agatgctgtt caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga
    10021 ttttgcaaca ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg
    10081 gaaaaagggt cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg
    10141 aagtgggaca gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt
    10201 agagataaag gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc
    10261 gttgaagacg tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat
    10321 tttatgtttt gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa
    10381 acaaatattt gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac
    10441 attgcacgca atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga
    10501 tgaaaacaat tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa
    10561 gaaattttct gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt
    10621 ccgttatggg gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc
    10681 acaaaatgtc agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga
    10741 ccggatgcat cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa
    10801 aatatttggg atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt
    10861 gaaacaagct gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat
    10921 tatcgctaaa agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt
    10981 aggtaatgct gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat
    11041 tccattacgt gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg
    11101 agctggagga ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat
    11161 tgattctcct gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca
    11221 ttttgctagt ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc
    11281 caaactagca ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca
    11341 agaagaaatc aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat
    11401 gttgcgtttg accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga
    11461 atcattaggt caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac
    11521 gaatacgcca atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc
    11581 agaacagatc gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt
    11641 tgctcctcca cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg
    11701 tttagcaata gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca
    11761 agcagaatcg tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa
    11821 aatagtgttt tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta
    11881 ccgtttttga actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg
    11941 aaaagccggt tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag
    12001 ttgataaatg atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg
    12061 gcagcttcaa gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt
    12121 cggcttattt ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc
    12181 tgccctgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg
    12241 gttgagctta ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt
    12301 ttccttccta cttgtaacct tgctcccgtg ttagaaacat gttacactaa atatgtgaaa
    12361 ttattaacaa ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa agcaactgcg
    12421 aaaagattac ctttatattt acgctacttg aagatgctgg atgactcagg tataagtaga
    12481 atcaaatcaa atgagttcag cgaaatcacc caaattcctt ctgcaacgat ccgcagagac
    12541 ttctcgcaat taggcgagct aggcagaagc ggatatggct atgatgtccc ttttcttatc
    12601 gatgtattca ataatatcct taatactaaa gaagaaaaac gaatcgcatt agtaggatat
    12661 ggaaatcttg gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa tctaaatatc
    12721 gtgtgcgtct ttgataatga tccagcactg atcaatcgag taattgatgg agaaatgatc
    12781 tatccgattg atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt agcaatctct
    12841 acggtaccaa gtaaatattc acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc
    12901 gctatactta attttgcgcc tgacagagta actgtcccag cttatgtcaa tgttcaatac
    12961 attgatttga caaccgagtt gcagacgctt atttactttg atgagaatta ctcagaagtt
    13021 ttttcttagg aaaatttgag taggcttatt gatcttgcga gaactcgtcg taattctttc
    13081 aaaaattttt tagtactaaa tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa
    13141 aaaccattgt agaatgaagc ataacgctga acaagaggag taactggcac attcattctt
    13201 agagagagaa ctatctgctg aaaggttctc aatgacaacc agtgaaggta gcttgggagt
    13261 ttctgtcttg aatggagtag agatagacga gtcgtgatcg ttaactcact tgagaggcaa
    13321 taatgattga ttattgcaat ttaggtggta ccgcgtaaat catacgtcct agaatacgaa
    13381 agtattttag gacgtttttt agtttaaagg aggaaaacag catgacggaa gaaaataacc
    13441 tatcaacaaa atataatccg caagaagttg aagctggacg ttatcagcat tggttagaac
    13501 aagatttgtt taaaccgagc ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc
    13561 ccccaaatgt tactggaaaa cttcatttag ggcatgcttg ggatacgacc ttgcaagata
    13621 tgctgattcg tcaaaagaga atgcaaggat atgatacttt atggctgcct ggtatggacc
    13681 atgcaggaat cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa gggatttctc
    13741 gttatgatct aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg
    13801 cgagccacat ccgcgaacag tgggctaaaa tgggcttatc tttagattac agtagggaac
    13861 gttttacatt agatgatgga ttatctgaag cagtacgtaa agtttttgtt accttatacg
    13921 aaaaaggttt gatttatcgt ggtgaatata tcatcaactg ggatcctcaa gcacgaacag
    13981 ctctatctga tatcgaagtt attcataaag atatcgaagg cgccttttat catatgagct
    14041 atgaactagc agatggatca ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt
    14101 tgggagatac ggcgattgct gttcatccag aagacgagcg ttatcaagcg ctcatcggca
    14161 aaaaagtgat tctaccttta gtcaataaac aaatcccaat cattgcagat gaatatgtag
    14221 atatggaatt cgggacaggt gtagtaaaaa tcactccagc acatgatcca aacgactttg
    14281 aggtaggtaa tcgtcatgat cttccacgcg taaatgttat ggatgatgat ggtacgatga
    14341 atgaactagc aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc
    14401 aagatctgaa agaaatcggt cgattgatta aaatcgatac gatgatccac agtgtgggtc
    14461 attccgagcg gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa
    14521 tggcgccgtt agcgaaaaaa gccattgata accaagaaac agatcaagca gtggaattct
    14581 tccctccaag attcaatcaa acgtttttac gttggatgga aaatgtccat gattgggtaa
    14641 tctcacgaca attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg
    14701 aaatgtatgt agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg
    14761 ttttagacac ttggtttagt tcagctttgt ggcctttctc aacaatgggc tggccagata
    14821 cggaaagcga agattataag cgatacttcc ctactagcac tttagtaacc ggttatgaca
    14881 tcattttctt ctgggtaagc agaatgattt tccaaagctt ggaatttact ggcgaacgtc
    14941 catttgagaa tgtcttgatt catggattga ttcgtgacga acaagggcgg aagatgagta
    15001 aatcattagg taatggaatc gatccgatgg aagtaatcga aaagtatgga gcagatgctc
    15061 tccgctggtt cttatcgaat ggttctgcac caggacaaga tgtccgcttc agctatgaaa
    15121 aaatggatgc ttcttggaac tttatcaaca aaatctggaa tgcttcacgt ttcgttatca
    15181 tgaatatcga aggaatgacc gtagaagaga tcgatttatc tggagaaaaa tcagtggctg
    15241 accggtggat ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc
    15301 gttttgaatt tggtgaagca ggtcgacaac tgtataactt tatctgggat gatttttgtg
    15361 actggtacat cgaaatgagt aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa
    15421 ccacccgtag catcttagtc catacacttg atcagatcct acgtttattg catccaatca
    15481 tgccattcgt tacagaagaa atatgggaac atattcctca tcagggaaat tctctagtag
    15541 tggcagaata tcctgttgta cgtccagagt ttgatgatga aacagcttct aaagggatgg
    15601 aagtattgaa agaattgatt cgagccgttc gaaatattcg ttcagaagtg aatacaccat
    15661 tgtctaaacc aattacgttg ctaattaaaa caaacgatgc taaaatcgat caatttttag
    15721 ttgaaaatac aagctatatc gaacgcttct gtaatcccga agagttgacg atatcaagtg
    15781 agatcactgc gccagatcta gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc
    15841 cgttagcagg attgatcaat attgaagaag aaatcaaacg attagaaaaa gaactggaaa
    15901 agtggacaag cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt tttgtttcta
    15961 acgcgccgga agaagtagta gaggcagagc gtgcaaaaga aaaagattat ttagagaagc
    16021 aaacggctgt caaagaacga atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga
    16081 gagtgtgaaa tcagctagct attactaaaa gctagctgat tgaacatcgt tacaggaaag
    16141 agtttgagcc tcactcttag cttcgattca taaggagatg tgacataaat tttgtcgcat
    16201 ctctttttta gtctactctt attatgtagg agaaggaggg aggtggatca tgtatcaaga
    16261 gtcaataaag tagttgataa aatcgttcct ctctctgttt cttttctctt tgtcatactt
    16321 ttatttttct tatgtgttct atttgttata ataaaagaga aatgggggaa ttgttgatga
    16381 cgattgaaga agcaatagaa tggatccata gtcgattacc atttggttca cgtcctggcc
    16441 ttgaccgagt cgaagcttta ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga
    16501 tccatatcgc tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga agcctattag
    16561 aagaggctgg gctgacagta ggtacattta cttcacctta tatcgaaacg ttcaatgaac
    16621 ggatcgcaat caatggcaaa agcattccag acaatgattt gatccagctt gtaaaaaaat
    16681 atcagccact gatcaaacaa cttgatcaat ttgaagcagt agctggtatt actgaattcg
    16741 agacattaac agcaatggca tttgattatt ttttgatgaa gaaagtggac gtagcgatta
    16801 tcgaagttgg cttaggcggc ttattggatt ctaccaatgt agctgtacca atgctgacgg
    16861 gaataacaac gattggactt gatcataccg aaatacttgg agatacaatt gaagaaatcg
    16921 ctgcacaaaa agctggaatc atcaaacaag gcgttccagt agtaacagga aatattgccc
    16981 cagaagcttt tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat
    17041 acggggaaaa ttataaaata aactatctgc atcctgatga gatgtgggga gaagtatttg
    17101 aattttttaa tgaaatagga aagatccctc aattaaccac tccgctttta gggcaacatc
    17161 aagctgaaaa tgcaggagta gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc
    17221 tgcctttccg agaaaaagat gttttgaagg gattgaagaa atctcgatgg ccaggaagaa
    17281 tggagcgttt gagtaacgag ccattagttg ttttagatgg tgcccataat acgcatgcaa
    17341 tgaaccggct tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc aacattttgt
    17401 tttccgcctt acgaactaaa aatgttagag aaatgctaga acgattgcag caagtaccaa
    17461 atgtccatat ttatttgacg acctttgaac atccaaaagc catcgaatta agtgaagtat
    17521 cagatttagc aagtgagaga gtatcaatcg tttcattgtg gcaattcgga ttagcagata
    17581 tattggaaaa gatgacaaat gaagacgtat tagttgtgac cggttcactt tatttcattt
    17641 cagaagttcg ccatttatta atacaattag gaggagatca tgaagtataa tgcagtgatt
    17701 tttgacatgg atggtttgct gttcgacaca gagttagtgt attacgaagc cacacaagta
    17761 gtagcagaca agatgggttt tccttatgat aaagacctgt atttaaaatt cttaggtgtt
    17821 tcagatgaag aagtatgggc gaattatcat atgattttct cggaatttgg caaaacaagt
    17881 gtacaaagat tcatagatga ttcataccag gaaacgcttg aacgtttttc gcaaggagcg
    17941 gtacaaatga agcccggtgt gttggaactc ctctcttttt tagaagaaca tcagattccc
    18001 aaagttgtcg cttccagtaa ccaacgagca gtcattgaac ttttattgaa gaagaacaat
    18061 ctgtttgaac gatttgagac gattgtttct gcagaaaatg ttcaacgagc aaaaccagac
    18121 ccagaaattt tcttgttagc taatcagaag ctaggtacag cgaagaaaga aacattgatt
    18181 ttagaagatt cacaaaatgg tattttagca gcttctgcgg ctgagatccc ggtagtcatg
    18241 gtaccagatc tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct
    18301 tctttacatg aaatccctgc attgttaaaa taaaaatcca tctcttttcg tatcttattt
    18361 tagataagat agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga agtacctact
    18421 agctctttac caagggaacg tatggaaatt tatggcgcgg aagctttatc ggatcaggag
    18481 cttttggcaa tcttactgcg taccggtcaa cacccttata gtgtgatgtc gattgctgga
    18541 aatctgttaa aaacgtttgg aggactggcc tctttacgac aggctacttt gcatgagtta
    18601 gaagagattc gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta
    18661 ggtcgacgaa ttcagacgga tcatgaaaga gcggctccac cggttcgtac cagctacgaa
    18721 ttggcccagc aattgattct ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt
    18781 tatttggaca cgaagaatca agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat
    18841 cagtcagtcg ctcatcctag agaaattttc cattatgctg tacgttattg tgcagcaagg
    18901 atcgttttag cgcataatca tccttctggt aatgttattc catcgcagca ggatatgaat
    18961 tttaccaaaa ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg
    19021 atcatcggac gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa
    19081 gttgctttct tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga
    19141 atagaagttg aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt
    19201 agtgcttttg gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg
    19261 ctgaaaaagg atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct
    19321 cagctatcca aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg
    19381 ttgaagaatc agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt
    19441 aaatcaaatt gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac
    19501 ctgccacaag agacaatttc tcttgtggca ggta
//
LOCUS       DAYYGS010000057        16533 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000057 DAYYGS010000000
VERSION     DAYYGS010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.057"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      <1..160
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            307..864
                     /locus_tag="ACHA44_001883"
                     /pseudo
     CDS             307..864
                     /locus_tag="ACHA44_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            922..2961
                     /locus_tag="ACHA44_001884"
     CDS             922..2961
                     /locus_tag="ACHA44_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HFM8905154.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFINNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3070..4221)
                     /locus_tag="ACHA44_001885"
     CDS             complement(3070..4221)
                     /locus_tag="ACHA44_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFM8905155.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4375..4905)
                     /locus_tag="ACHA44_001886"
     CDS             complement(4375..4905)
                     /locus_tag="ACHA44_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HFM8905156.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(5004..5354)
                     /locus_tag="ACHA44_001887"
     CDS             complement(5004..5354)
                     /locus_tag="ACHA44_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HFM8905157.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5511..7040)
                     /gene="ahpF"
                     /locus_tag="ACHA44_001888"
     CDS             complement(5511..7040)
                     /gene="ahpF"
                     /locus_tag="ACHA44_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFM8905158.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(7050..7613)
                     /gene="ahpC"
                     /locus_tag="ACHA44_001889"
     CDS             complement(7050..7613)
                     /gene="ahpC"
                     /locus_tag="ACHA44_001889"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFM8905159.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7757..8419)
                     /locus_tag="ACHA44_001890"
     CDS             complement(7757..8419)
                     /locus_tag="ACHA44_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905160.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8603..10249
                     /locus_tag="ACHA44_001891"
     CDS             8603..10249
                     /locus_tag="ACHA44_001891"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8905161.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10240..11322
                     /gene="uxuA"
                     /locus_tag="ACHA44_001892"
     CDS             10240..11322
                     /gene="uxuA"
                     /locus_tag="ACHA44_001892"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8905162.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11430..12983)
                     /locus_tag="ACHA44_001893"
                     /pseudo
     CDS             complement(11430..12983)
                     /locus_tag="ACHA44_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            13131..14272
                     /locus_tag="ACHA44_001894"
                     /pseudo
     CDS             13131..14272
                     /locus_tag="ACHA44_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14398..15477)
                     /locus_tag="ACHA44_001895"
     CDS             complement(14398..15477)
                     /locus_tag="ACHA44_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8905163.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15580..16020)
                     /locus_tag="ACHA44_001896"
     CDS             complement(15580..16020)
                     /locus_tag="ACHA44_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8905164.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16077..16487)
                     /locus_tag="ACHA44_001897"
     CDS             complement(16077..16487)
                     /locus_tag="ACHA44_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8905165.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
BASE COUNT         5647 a         3050 c         3032 g         4804 t
ORIGIN      
        1 ttgttaggtg aggctcctat acaaacatag gctgttgccc agaaacgtcg aaagtcgcca
       61 atgggtaaaa cagaaactgt tggattaagg ctttttccaa tagctaagaa tgttttcttt
      121 acgttgtata gtgctaaaac tcggacgatg agatgatgct aggtcagaat tcttgttcga
      181 aaaggtttct gaccttttct ttatattttt gtgactgact tatttgatga gacaaactcg
      241 ctcctcacct gacaagtgga ttgctgtgtg aggaggagat gcggtgaaaa aagatcgatt
      301 ggtggaggtc gctcggattc acttctggtt gcacaataca accagaaaac acaacaagca
      361 aaactgatct ctatcatgag agattcatat gtcgatattc caggttacgg aatgaataag
      421 ataaatgcag cttactctta tgggggagtt gatttattaa atcaaacatt aaaggaaaat
      481 ttcaaatttg aagctcccta ttatgcaagt atcacatttc aagattttat cgattgcgtt
      541 aatgaactgt ttcctggtgg agtcaagatc gatgcagaaa aatctctaga tttagatggc
      601 gtatatataa acaaaggaga gcaagtgatg gatggtaata ctttactcca gtatgctcga
      661 tttcgggaag acaaagaagg agacttcggg aggatcagaa ggcagcagca agtgatcaaa
      721 gctatctctc agcagctgaa agacgtaact tcaatattca agctacccaa agcggttggg
      781 aaattacttg gtagtatcca aacgaacctt ccagaaagtg tgcttctgga ttgtggtatg
      841 gatttcctca aagacatatg ttagaataaa caggtataaa tagtgaaata aaggaatgac
      901 aagcaagaga aggaggaaaa aatgaaaaga agtgacaagc acggcaaaaa tcgaacaggc
      961 gcttatattg ccggcgcagt gatcttaata gcagctgcgg gtggcggtta tttttactac
     1021 cagcactatc aagaaaccca agcagtagaa gctggagaaa agacagttga gcaatttgtc
     1081 caagctttga acaagggaga ttataacaaa gctgcagaaa tgacatcaaa aaaggcggca
     1141 aataaaagtg cattatctga aaaagagatc ttagataaat accagaatat atacggtgct
     1201 gctgatgtca aaggacttca gatatcgaat ctaaaagtag ataaaaaaga cgattctact
     1261 tacagttttt catataaagc aaagatgaat acctcattag gtgaattgaa agatctttct
     1321 tataaaggaa cattagacag aaatgatggt caaaccacga tcaactggca acccaacttg
     1381 gtttttccag aaatggaagg aaatgacaaa gtaagcctga ccacacaaga agcagcaaga
     1441 gggaatatta tagatcgaaa tggtgaacca ttagcaacaa caggcaaact aaaacaatta
     1501 ggagtcgttc caagtaaact tggagatgga ggcgaaaaaa cagccaatat caaagcgatt
     1561 gcttcctcct tcgacttaac agaagatgct atcaatcagg cgatttcaca aagctgggta
     1621 caacccgatt actttgtccc attgaaaatc attgatggag caacgccaga acttccagct
     1681 ggagctacca ttcaagaagt agacggcaga tattatcctt tgggtgaagc agctgctcag
     1741 ctgattggtt acgtgggaga tatcacagca gaagatattg ataagaatcc agaattaagc
     1801 agtaatggaa aaatcggtcg ctcaggtttg gagatggctt ttgataagga tcttcgtggg
     1861 acaacaggtg ggaaattaag cattacagat gcagacggtg tagagaaaaa agttctgatc
     1921 gaacatgaag ttcaaaatgg caaagatatc aaattgacaa tcgatgcgaa ggcacaaaaa
     1981 atagcttttg acagtctagg aggaaaagct gcatcaactg ttgcgacaac gccaaaaacc
     2041 ggtgatcttc ttgcgcttgc tagctctcca agctatgatc caaacaaaat gacaaacggg
     2101 atctcacaag aagattacaa agcttatgaa gaaaatcctg aacatccatt catcagccga
     2161 tttgcgacag gttatgctcc tggatctacg tttaaaatga ttacagcagc aatcggtctc
     2221 gacaacggca ctatcgatcc aaatgaagtg ttgacgatca acgggcttaa atggcaaaaa
     2281 gacagttctt ggggatcttt taaaacaacg cgtgtcagta gtgatgtatc acaagtagac
     2341 ttaaaaactg ctttgatcta ttccgataat atatatgcgg cacaagaaac gttgaaaatg
     2401 ggtgagaaaa aatttcgtac aggcttagat aaattcattt ttggtgaaga ccttgatttg
     2461 ccaattagta tgaatccagc acaaatttct aatgaagata gctttaactc agatatcttg
     2521 ctagctgata ctggatatgg acagggcgaa cttctaatta ctcctatcca gcaagcagca
     2581 atgtattctg tttttatcaa caatggcaca cttgtctatc ctaaattgat tgcagataaa
     2641 gagacaaaag ataagaagaa tgtcatcagc gaaacagcag tacaaacgat cgtgccagat
     2701 ctgagagaag ttgtgcaaga tgtaaatggt acagcacatt ctctttctgc tttagggatt
     2761 ccgttggcag cgaaaactgg tacagcggaa atcaaagaaa aacaggatgt aaaggggcaa
     2821 gagaacagtt tcttgtttgc tttcaatctt gataaccaag gttatatgat ggtcagcatg
     2881 ttggaaaata aagaagatga tgattcagca accaaacgag catcggaact attgcaatac
     2941 ctcaaccaaa attatcaata aaaaattgct ttttaagtac agtaaaagga ctgtggcgtt
     3001 atcgtcacag tccttttttc aaataaacgg ttttcacgat gtctctttca caacttcttt
     3061 ttgatttact cacacttcat aaactttttt tgtaaaatat ttgagaacaa gtggagaaat
     3121 aagtgtactt aataaaatca cgataactaa aggtgaatag tactgtggtt cgatcaattg
     3181 actttgctgt ccaatttgta aaatgatcaa agccatttct ccacgcgaaa tcataccagc
     3241 accgaccatc aatgaactat gttgagaaaa accggcaatc tgagcaccaa aataacctcc
     3301 aagcaatttg gtcaaaatcg ctacaagcgt caatatagaa ataaagagta gttgttcact
     3361 aaagtcagaa aaatcaactt ctaatcctac actgacaaaa aatactggta taaatactgc
     3421 atatcctaaa gcctctacat tgtggtatac ttcttcttta acctttgtct gactgacagc
     3481 tatcccagca aaaaaagcac caatgacaga gctaagccca acaagatccg ctacgtaaga
     3541 cattcctaaa caaataacta gcgatacgat gataatagaa gaatttgcgt acactttttc
     3601 tgcaaatgaa agtaggaaag gcgcaatcca tttgactaat aagaaaataa aaacaaaata
     3661 aattagctgt tcaatcaaaa caagcggtaa agaagcattg ctagaagctt cccccgttag
     3721 aaaagatagg ctgaaactta gaactaaaac gactaaaatg tcatctacca cagaagcacc
     3781 caaaattgtt gatccttctt ttgtattcac tacgtttagt tcttttaata cttctactga
     3841 aatactgaca gaagttgccg caagaataat cccaaagaaa atcgcttcat ttcccgaaac
     3901 ttggaatgct ttgcctgtca accagccaaa aacaaccggg aagatgatcc caagcaaagc
     3961 aacgaacata ccaggtcgga aatatttttt caaaagagat aaatcacttt ccagaccagc
     4021 taaaaacatt aataagatca caccaatctc cgaaaagtca tggactagaa tatcaggttt
     4081 gacccaattc aatccagctt ggcctagcaa aacaccaaca agtaattgtc caatcactgc
     4141 tgggatacct aatctccgcg agatatgtga gccaatcgtt gttgaaatca atatcaggca
     4201 taaaatacca ataaattcca ttctcatcat tcctttgtat aaaatatagc ccgaacttag
     4261 cataacatac attattgaaa aaataggaaa atatactcac aaaaaaacca acaatccaaa
     4321 agattttttt ggattgttgg tttttatacg gtctatttta ataactaaaa atgattaacg
     4381 tatagtcatt tttcttattc tgatgaaatt gataatctcc tggatgccca tgaaaatcaa
     4441 aataaagccg aatatttgga atagaagcat caaacttttg aatggattga acaataagac
     4501 gaatccgcca atcaacaaca gaatactact ataaacactc caacttttag cttttgtatt
     4561 catgctgaag aacaactgga acaagctgtt gatgataaca gctaatccaa gaagaatcgg
     4621 caggatagaa acgattgcag gtgcaaaagc atagacgatc agagctaaga tcaggaaaaa
     4681 aagaccactg aacagaccga tactccaaga acctgttttc cttttaaaac ggtaagcctc
     4741 aaaaagattc aacaacccaa gcaaaacaag ataggcggtg atcagataac cgataaaatg
     4801 aaaaacagca gaagggttta gcacaatcgc aatcccagaa ataatataaa cagcagctct
     4861 taataaggcg tatttttgaa tattttgttt aatcgtctca aacatataag aaaacctcct
     4921 ttgacttatt ttaacgtgaa ctagtcattt taccaaatta aatgttacaa aatgagcgaa
     4981 ggagaagaat atctctggaa aaatcaactt ctgaggatct tttccatttt tttgcctttt
     5041 gctagctcat cgatcatttt atctaaataa cgaatttctt tcatcaagtt atcctcgatg
     5101 tcttctactc gtacaccaca aacaacacct gtaatcaagg aacgtgaagg attggggaca
     5161 ggggcttccg caaaaaattg ttcgaaagtc acttgttttt gtagttgata ttctagttct
     5221 gtttgggaat aacccgtcag ccaacaaatg atttcatcta cttcctcttt tatacgatct
     5281 tttctctccg cttttgctac atacatagga taaacgcttg caaaacttgt cgtaaaaata
     5341 cgattttgcg ccataatcag tcacctttct ttttttcaaa ttatttacta cttatagtat
     5401 ataagaaaaa gagaagaaaa agcgattatt caaaaaaaca aaaaggattg tgacaaaatt
     5461 cttgtcacca tccttttttc cgctctctga ctttttacaa tcttcatagc tcaagtgcgc
     5521 atcagataat cgaatgctcc taaagctgca gtagctcctg aacccattga aacaatgatt
     5581 tgtttgtatg gactatctgt acaatcacca gctgcgaaaa cgccggggat attcgttgcg
     5641 ccatttttct cggtcactat ttcatttctt ggattcattt cgatcgttct atctagccaa
     5701 tccgtattag ggagtaagcc gactagaatg aatactcctg aaacagctag tgtatgcatc
     5761 tccatcgtac ggcgatcgat ataggagaga gattctacct gattcgtacc gctgatttct
     5821 ttcgtcgctg catgtttgat aacagtgaca ttcgcaagag agactagttt atcctgtaag
     5881 atttgatctg cttttaattc tggtaagaat tctagaacat atacatgttt tgctaaccca
     5941 gccaaatcga ttgctgcttc aacaccagaa ttaccaccac cgataacagc aatctctttt
     6001 cctttaaata aagggccgtc acagtgtggg caataagcaa ttcctttatt tttaaactct
     6061 ttttcgcctg gtacattgat agaacgccag tgcgcacctg tcgcgatgat ggcggttttt
     6121 gctttaagtg atataccgtt agctaagcca atattaataa aatgttgctc tttatttaag
     6181 gaaacggcct gttgattcat cataatgtcg atagggtaac tacgcacatg actttctact
     6241 tgctgcatca attttggacc ttctgtgtaa gaggtaccaa tcatattttc aatccctagt
     6301 gtttcaacga cttgtccgcc gaaagaatca gcgacaagtc ctgtacggat tcctttacgt
     6361 gctgcataga tagctgcact actagctgct ggtccaccgc caatcactaa tacatcataa
     6421 ggttcttttt caaacaactc ttcttcaatc aaagggccag tgattttttc aacaatttgt
     6481 tcgatcgtca tgcgtccgct actaaattct tctccattta aaaacacagt tggcactgcc
     6541 atgatttttt tatcatcgat ttctttttgg aacatcccac cttcaatcat cgtatgagta
     6601 atatttggat ttaatacagc taatatattc aatgcctgta caacgtcggg gcaattatga
     6661 caagtcaagc taacataact gtcaaaatgc aatgtttgtg ttattttttt gattttaaga
     6721 ctcaaagatt cactgatttt cggtgcccgt ccactaattt gtaacatagc cagtaaaaaa
     6781 gaggaaaatt catgaccgag ggggatacca gcaaaaacga ttccactttc tttgccaact
     6841 ggattgatag aaaaactagg tgtgcgcatc gaatctcttt tttcccaaga gattttagga
     6901 gacatttctg ctatttcctt cgtaaattca gctagttttt gactattttc actattatcc
     6961 aaactaacag aaaaaacaat aggagattca atcagtgcta aatattgttc gagattttgt
     7021 ttagtttctt tgtctaacat atccaccacc taaatctttc caacaagatc aaaacttggt
     7081 ttcaatgttt cgccactttc tttccatttt gccggacaaa cttcacctgg gtgggtacgt
     7141 acatattgag cagcgcgaat cttatcaatc agtgcactag cgtcacgacc gattccatct
     7201 gcattgattt ccatagcttg aacgattccg tctggatcaa taataaatgt tcctcgctga
     7261 gctaatcctt gctcttcatc caaaacatca aaaatacgag aaatctgatg agacggatca
     7321 ccgatcatca cgtattcgat tttaccgata gcatctgatg tatcatgcca tgctttgtga
     7381 gtaaagtggg tatcggtaga gcaagaaaag acttcaacac ctaaagattt caatgtactg
     7441 tattgttctt gtaagtcttc taattcagta ggacaaacaa aagtaaaatc agctggatag
     7501 aagcaaagaa tactccagtt ccccataaga tctttttcac tgacttctaa aaattctcct
     7561 tgatgataag catgtgcatt aaattcttcg atttttttcc caatcaatga catagtaaaa
     7621 cctccaataa taattattta tttataatta ttgtaattta gttggagttg caaagtcaat
     7681 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
     7741 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
     7801 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
     7861 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
     7921 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
     7981 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
     8041 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
     8101 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
     8161 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
     8221 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
     8281 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
     8341 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
     8401 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
     8461 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
     8521 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
     8581 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
     8641 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     8701 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     8761 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     8821 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     8881 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
     8941 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
     9001 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
     9061 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
     9121 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
     9181 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
     9241 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
     9301 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
     9361 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
     9421 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
     9481 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
     9541 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
     9601 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
     9661 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
     9721 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
     9781 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
     9841 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
     9901 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
     9961 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
    10021 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
    10081 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
    10141 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
    10201 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
    10261 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
    10321 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
    10381 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
    10441 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
    10501 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
    10561 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
    10621 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
    10681 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
    10741 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
    10801 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
    10861 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
    10921 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
    10981 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
    11041 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
    11101 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
    11161 gacaacggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
    11221 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
    11281 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
    11341 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
    11401 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
    11461 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
    11521 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
    11581 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
    11641 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
    11701 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
    11761 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
    11821 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
    11881 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
    11941 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
    12001 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
    12061 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
    12121 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
    12181 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
    12241 tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa
    12301 ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg
    12361 tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca
    12421 tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat
    12481 tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac
    12541 aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca
    12601 ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa
    12661 gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag
    12721 taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc
    12781 agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa
    12841 tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt
    12901 tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt
    12961 ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg
    13021 aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat
    13081 ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt
    13141 caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag
    13201 tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc
    13261 gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag
    13321 gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac
    13381 aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca
    13441 ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact
    13501 tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt
    13561 taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag
    13621 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
    13681 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
    13741 aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag
    13801 atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag
    13861 acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag
    13921 atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca
    13981 aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa
    14041 aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg
    14101 aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct
    14161 accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag
    14221 tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa
    14281 taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata
    14341 ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta
    14401 agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa
    14461 aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct
    14521 aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt
    14581 cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac
    14641 aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat
    14701 aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg
    14761 atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac
    14821 aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat
    14881 atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt
    14941 tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat
    15001 ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa
    15061 aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg
    15121 ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat gaataagcat
    15181 cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat
    15241 agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat
    15301 caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag
    15361 aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg
    15421 ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat
    15481 atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
    15541 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
    15601 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
    15661 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
    15721 accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
    15781 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
    15841 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
    15901 aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt gtctaatgat
    15961 ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
    16021 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
    16081 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
    16141 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
    16201 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
    16261 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
    16321 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
    16381 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg cacttctcca
    16441 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa attaccacct
    16501 ttaattatat tttatacaat tgaattttac aca
//
LOCUS       DAYYGS010000058        16512 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000058 DAYYGS010000000
VERSION     DAYYGS010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16512
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.058"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(48..245)
                     /locus_tag="ACHA44_001898"
     CDS             complement(48..245)
                     /locus_tag="ACHA44_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8905166.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(639..2366)
                     /locus_tag="ACHA44_001899"
     CDS             complement(639..2366)
                     /locus_tag="ACHA44_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HFM8905167.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2359..3552)
                     /locus_tag="ACHA44_001900"
     CDS             complement(2359..3552)
                     /locus_tag="ACHA44_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HFM8905168.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3739..4383)
                     /locus_tag="ACHA44_001901"
     CDS             complement(3739..4383)
                     /locus_tag="ACHA44_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905169.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4477..4611)
                     /locus_tag="ACHA44_001902"
     CDS             complement(4477..4611)
                     /locus_tag="ACHA44_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HFM8905170.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4613..6745)
                     /gene="feoB"
                     /locus_tag="ACHA44_001903"
     CDS             complement(4613..6745)
                     /gene="feoB"
                     /locus_tag="ACHA44_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HFM8905171.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6742..7215)
                     /locus_tag="ACHA44_001904"
     CDS             complement(6742..7215)
                     /locus_tag="ACHA44_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HFM8905172.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7616..7840
                     /gene="nrdH"
                     /locus_tag="ACHA44_001905"
     CDS             7616..7840
                     /gene="nrdH"
                     /locus_tag="ACHA44_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFM8905173.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            7999..10158
                     /gene="nrdE"
                     /locus_tag="ACHA44_001906"
     CDS             7999..10158
                     /gene="nrdE"
                     /locus_tag="ACHA44_001906"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFM8905174.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10208..11173
                     /gene="nrdF"
                     /locus_tag="ACHA44_001907"
     CDS             10208..11173
                     /gene="nrdF"
                     /locus_tag="ACHA44_001907"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFM8905175.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11262..12401)
                     /locus_tag="ACHA44_001908"
     CDS             complement(11262..12401)
                     /locus_tag="ACHA44_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFM8905176.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12710..13423)
                     /locus_tag="ACHA44_001909"
     CDS             complement(12710..13423)
                     /locus_tag="ACHA44_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFM8905177.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13677..14378)
                     /gene="nagB"
                     /locus_tag="ACHA44_001910"
     CDS             complement(13677..14378)
                     /gene="nagB"
                     /locus_tag="ACHA44_001910"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFM8905178.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14612..16144
                     /locus_tag="ACHA44_001911"
     CDS             14612..16144
                     /locus_tag="ACHA44_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8905179.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16335..>16512)
                     /locus_tag="ACHA44_001912"
     CDS             complement(16335..>16512)
                     /locus_tag="ACHA44_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8905180.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5120 a         3412 c         2863 g         5117 t
ORIGIN      
        1 tatcattata taattcatga acaatttgct tagcattttt tttcatttta gcacttccct
       61 ggaattgctc cacctaaaaa accacctata gacattcctg caatacaagt agttgccttg
      121 gcccaatcga ccgtacattt atttttagtg caatacactc catttccata atattttcca
      181 ctatgagtgg tacccccata gataagttgt gtctctttaa tagacaaaat ttttaaatgt
      241 ttcataatct aatcacccct aaattaatgt ttttagacta ttattctaac attcattaaa
      301 tttcaaaaaa caattccttc ttgaaacgta gcaaaataat tcctgaataa gaatttaatc
      361 acttttaagt atataaacta tgtatattct ttaatatttt ttgagaatgc acttcgtaca
      421 taataaactt taatcacatt agtctttcat acctacgatc gatctctttg actcgcaccc
      481 ataaattcat aatagtttgt cggcgtcttt gaacgtttcg aagatgagct acgcattctc
      541 tttttttata gacaaaaaag atagagacga acatggagct tccaaatttg gatatttgat
      601 ccaattttgg aagctccatt tatttcgtgt ctgtcttttt attttttcca gctttcgact
      661 aattcttggt tctctttgat ccagtctttt gctgcttgct gtggatcttt tccattgttg
      721 atttccaaca taactttttc catatctttt tctgaccaat ggaaattgtc taatactttg
      781 taagcttctg gctggtcttc tttcaatcct tttcgaacca tcgtatggat ctgctcttcg
      841 cttcccattc cgttttcagg atcttctaag tatttcagat catatttggc aaacatccaa
      901 tgaggtgtcc atcctgtaac tacaattggt tcgtgttttt tgatcgcttg acctagtgca
      961 acggtcattg ctcctgaaga agatgtctct actttccagt ctttcaagtt gtcatatttt
     1021 tggatcgtgt tttcagcagc cgtaactaca ccagctcctg gctcgattcc tatgattttc
     1081 ttacctgcct gatcggttaa ctcatcgatc gaatttacat ccatgtaggc tggcactgct
     1141 aaaccaactt ttgctcctgt caaattggca cctaggtctt cgacttgatc tttatattgt
     1201 gcatattgtg aaccatgagt tttaggtagc caagctgaaa ccatggcatc tgactcgcca
     1261 tttgaaacag atttccacat gattgaatta tctaatggcg tcatcgttac atcatagccc
     1321 atttgtttca atacttcacc gactacattt gttgaagcaa cttctgtatc ccattcaaca
     1381 taagataagt tgatacgttt tgtttctttg gcagatgaaa aactccctat accaatcgca
     1441 cctaagatca ctactactac agcaccaatg atcagtcctt gacgtttttt acttttctgt
     1501 gttgttgctc ctgcagcttt tttattgttt ggctggttca aatgctgtgt aaaacggtca
     1561 atgatgattg ctaagataac taatgcaacc ccgttgacaa atccattccc gacttgtgct
     1621 ctttgcaatg ctgataatac gccgcgtcct aagccgggtg caccgatcat agaggcaata
     1681 acgaccattg aaagtgacag cattgttgtt tgattgatcc ctgccatgat cgtactttta
     1741 gctaaaggca attctagttt gaatagtttt tgacgacttg tgctcccgaa tgaatcagaa
     1801 gcttctacta attctttagg aacttgtcga atccctaaat tcgtaaaacg aacggttggt
     1861 ggcagagcaa agatgactga ggcaaatacc cctggaacca tcccgatacc gaaaaatgca
     1921 actgctggaa tcaaataaac gaatcctggc attgtctgca taaagtcaag gatcggtttg
     1981 ataatacttt gggctttact gctttttgcc atcaagatcc ctaatggtac cccaatgatg
     2041 atcgagatca cactagataa aagaacaagt gtgacagtat tcatcaaatc attccataga
     2101 ccttggttat aaataaacca tagaccgatt aatgtgaaca acgtaagtcc tggacgtttc
     2161 ttagaaataa agaaagctgc taccgtcaat aacaagataa acaaaggagc tggaatcact
     2221 aacagtaaat ttgtaatccc cgacatcact gcctgtccaa tcgtttgtaa aaatgaaaat
     2281 aggccagaaa aagttgtcgt aaaccattct gtgatatttt ctacccagtt tgccactggt
     2341 aattgatagt tcaatagatt attcatgttc actcacctcg cttgtttctg ctaatgcttc
     2401 aatcacacta cctcggatga cgacaccttt taattttcca tcctctacaa ctgcaagtgg
     2461 agtcggtgag tcataaataa tattgaaaat atcacttacc accatgtctt tcccaatcac
     2521 cgtcacattt tcatctagga agtcgatcaa tggacgtttt tgcttcctag cttctaacgc
     2581 ttgatctgca gtgatgatcc ctttcaggtg tctctttctg tcaacagcca tcagcatgct
     2641 gacttcttca ttacgcatcc ttgtcaatgc aacgtttggt ccatcagatt cgatattcgt
     2701 cgtcaatgca ggtaccatga tgttttgagc cgtaagcact ttggaacgat cgacttcttc
     2761 cacaaattca cgtacataat catttgctgg attggtcaat atttcttcac ctgttccgat
     2821 ctgcatgatt tcgccgtctt tcatcaaggc gattctgtcc ccaatccgta aagcttcatt
     2881 caaatcatgg gtaatgaaaa taatcgtttt ttgaacattg gcttgcaggt ccagtaattc
     2941 gtcttgcatt tctcgccgga tcagcggatc aagagccgag aacgcttcgt ccatcagcaa
     3001 gatctcagga tcatttgcta aagctctggc taggccaacc cgttgttgca tcccaccaga
     3061 taactgacta ggatattggt ctttaaatga aagcaaacta gaattttcta atgctttttc
     3121 tgctttttct tgtcgttctt ctttaggtac accacggact tctaacccgt attctgtatt
     3181 ttccaagatc gttcgatgtg gaaacagacc gaagttttgg aaaaccatat tgattttatg
     3241 acgacggact tctcgcaatt cttctttgct taacttcgat acatcttctc catcaatata
     3301 gatactgccc gaagttggct caatcagacg attcaataga cggatcaatg tggatttccc
     3361 actcccggaa agccccatga tcacgaagat ttctccttct tttacgtcaa aattcacgtc
     3421 atacacacca accgttgcac cggttttctt taaaatctcc gttttcggct ggtgatcatt
     3481 catcatatct aaagcggctt gtgtcttttt accaaatatc tttgttaaat gttttacttc
     3541 tacttttacc aaacaaattc tcctttttta tgtaaattca tttttctttc tgaaaaaagt
     3601 gaccacacta tgttaacatc atgtagtcac tttgtcaaaa atgaaagaaa tttgcttgtc
     3661 caaactgaaa aaaacttggc taaatctttg taaataaaag gtttagccaa gttaatattt
     3721 ttttaatatt tcacacaatc attctgcaag ctgatgctcc gggtaaaaaa agtttgtcat
     3781 acgctgtagt gtatattcat ttccaataaa atgaacgatg tcccctgccg taaatttagc
     3841 ataggggcca ggtgaaacaa gcagttcttc attatgagaa attgcgataa ctgtggcgcc
     3901 agtcgcctgc cagatattca actcgctaat tgaacggtcc agatgcattg ctttttctga
     3961 taaagataat tcaaacggaa gtaaaggatt catcttatgt attcgcttcg tttgattgac
     4021 cagctgatcc aataattgag aaaaatgatt cagctcatct tgttgcttct ccacacttgc
     4081 catgatttct gcttttgtat cttggatgga ttgtacatct tgatattgca ataggaaatc
     4141 ttttgctttt tccttcgaag caacaaatgc cccacttcca tgccgtactt ccatgattcc
     4201 aagatctacc agcacattga tagccttgcg ggtagtctca ggagaagcat tgtacgtact
     4261 ggcaagtgtt gagcgggcat ggatcttctc acccacttta tatcggtttt caacgatacg
     4321 ttcggctaaa tcaacagcaa tctgctggta gcgcgggcgg acgatttttt tctttccaga
     4381 cattttcttt cctctttctt tttttacttt ccctcatctt actatgctct agcaaaataa
     4441 gcaaaaaagg ggagaatagc ctcttgcaac gactgatcat cgcttgatct gttccttttt
     4501 tactgggcaa gctgtttgac agtcatcaca attcttaccg gattttaaac gagtatagac
     4561 aattgcgcct gcacttccaa agattaacac acttaataag aatgtcgcca tcctattcct
     4621 ctccttctaa tgacaatata gtataaaact ggttcttgga ttttggctga cggaacaaaa
     4681 cataaacgag tcctatcatc agtaaaatag caagatacgt ccctaacgct acctgtccac
     4741 cctccaataa gtgtcccaac tgataaatca caaaactaac taaataagct aaaccgcatt
     4801 gatagcctat ggcgatccaa gtccatttct tggcattcat ttcccgatgg atagccccaa
     4861 ttgccgcaaa acaaggcgca caaagtaaat tgaaggctaa caatgaataa gctgctgcgg
     4921 gagtatatgt gtgttgtagc acttgccaga tttctctgcc attttcggat acttccgcat
     4981 gaccaaacaa aataccaaat gtgccaataa tattttcttt agcgaccaat cctgtaatcg
     5041 ctgcgactgt tccttgccag tttccccaac caagcggctg aaaaacaact gccaggattc
     5101 gcccgaaaaa ggctaagata ctttggtctt cagaaacagg ttgagcataa aaattataag
     5161 aagagctgaa ccagatcacg atactagaaa caaagataat cgttcctgct tttttaacaa
     5221 aagacttgct gcggtcatag gtttgtctta ggaccgctga ccattgagga aaatgatatg
     5281 cgggcaattc catgataaaa ggagctgtat ctccagcaaa caaacgtgtt tttttcaagc
     5341 tgattccaga gaaaataatt gcagtcatac caagaaaata ggcagaagga gctacccaag
     5401 attgatgagg aaaaaaggcc cctgctacta aggcaataat cggcaacttt gcagaacaag
     5461 gcatgaaggt tgtgaccatg atcgtcaaac gacgatcttg ctcgttttca atcgtgcgac
     5521 tggccatgac gcctggcact ccacatcctg tagcaatcag cataggaatg aaggattttc
     5581 ctgaaagacc gaatttcctg aaaagtcgat ccatgacaaa agcgatcctc gccatatatc
     5641 cgcagtcttc cagaaagctc aagcataaaa acaatataat caattgcggt agaaatccta
     5701 acactgctcc cacgcctgcg atgatgccgt tcaagatcag ctcctgcatc catcccgcta
     5761 cttgccattc agctaacaat ctgccaacat gttctggtaa ccatgtacca aacaatgtgt
     5821 cattgatcca atctgtcccc attgttccta ctgtttgaat agccagataa tagatcagcc
     5881 acatcacaaa tgcgaagatc ggcaaggcta accaacgatt cgttgcaatc tgatcgattt
     5941 tgtcactcat cgataattgg aaagaatcat ttgacacaac acagagtgta catagttggg
     6001 taataaattc ataacgttca ttgaccagaa tcgtctcact atcgtcgtcc aatagtttct
     6061 ctgttaactg gatcagttgc tcgatctctt tttgctgaga aactgctatc tcgtaatcct
     6121 ctcttacctt ctgatctcgt tcaaaatatt tcattgcata ccagcgtttc ttgccgcttt
     6181 gttctcctag tacatcttca atttcatgta aagctgcttc taaacgtgga tcatatgtac
     6241 agggaacagg aatagaaggc cgcttttttg cagactcctg aatcttttcg attagttttt
     6301 cgattccttg ctttttcaat acgctgattg gtaaaactgg tacaccaaga ccataggaaa
     6361 gcttttctag attgattttt tttccttctt ttttgatgac atccatcata ttcagaccaa
     6421 tgatcatagg aatgtctaaa tccaataact gcgtagttaa ataaagattt cgttctaaat
     6481 tggtagcatc aataatgttg atgatcagtt caggctgttc ctgaaccaaa taatctcttg
     6541 caatcacttc ttctggtgta taaggagaca aagaatagat gccgggtagg tcctgaatat
     6601 gaatgttcgg attttttttc aacgatcctt cttttttttc tactgttacg cccggccaat
     6661 tccctacata ttgattcgaa ccagtcaaga gattgaacaa gctagttttc ccgctgtttg
     6721 gatttccgac taacgcgata ttcattcttt ttcctccctt tgtatcaaga ttagttcggc
     6781 ctctgacttt cgtaacgtca gttcataccc tcgcactgtc agttcgattg gatctcccag
     6841 aggggctagt tttcggataa ctatttttac atttcgcgtc aatcccatat ccattaaccg
     6901 tcgcttcact gcgccttgtc cgtggattgc tacgacgatc ccagtctctc ctgcttttaa
     6961 ttgatcaaga gaagtccagc tttcagagga agcagtcttt ttattgacaa agatttgacc
     7021 tagtatttcc cgacttactg ccactcgatg actttttacc agtaaaatgg cattgttgcc
     7081 tgataaattg acgacgacga ttttacttcc ggcaacgatc ccgatatttt gcaaatggcg
     7141 ttttgttttt tcttctgttt cgattcgttc gacttgataa acgaaattct gctttgcctg
     7201 cgctaaggtc tgcatattga ttcctctctt gtgaaataaa tcatgtatta aaatcattca
     7261 ttatatgttc taattttctt acgtgttacg caaataaatg agaatcattc tcacatagaa
     7321 tactctcggt aattgagaat gtcaattata cttgtgatat tatgagcgaa tctttagtat
     7381 tgatagataa aaatgagaaa aaaatatttc ttcatagttt tccacaattt tttctctatt
     7441 aaatttgcta ttttcccaca cgatacttgt tttcagctct tttttcaaaa acacaaaact
     7501 tagtattcca cagtttccac aacacaatat gtagtgtttg ttgtttgact tttttactaa
     7561 gagttttata ctatctatta tagataataa gaatgtgtta gaggaggcac tcaccatgaa
     7621 cgtaaaaatc ttttctaaaa ataattgtat gcaatgtaag atggtcaaac ggtttttaac
     7681 agaaaatcaa attgcttttg aagaagtcaa tatcgatgaa cagcctgaag cagtagactg
     7741 gttgaaagca caaggattcc aaagtgttcc tgtcattact tctgatgcag aaacagttat
     7801 cggattccgt cctgatcagt tgcgtaaact tgccagctaa catgactaaa agaatcaaaa
     7861 cgtgatacat ggatttgaag ggcttgagac agcaccagtt tttgtcgcaa acttccaaat
     7921 ccttttttaa agcttaaaaa caatgcaatg atttgcaatc aagcatcatc aaatacgaaa
     7981 tgaaggttgg gcgtacgtat gagtcttaaa aatttacaag atgtcagtta tttcaagctg
     8041 aataatgaaa tcaatcggcc agtcaatggt caaatccctt tgaataagga taaagaagct
     8101 ttagttgcct tttttgagga aaatgtcaaa ccaaatacga tgacttttcc atctattatg
     8161 gaaaaaatca attacttgat cgatcaagaa tatatagaag cagaattcat tcaattgtat
     8221 tcaccatcat ttattgaaaa gctatatcaa tttttggacg agcaacattt ttctttcaaa
     8281 tcttttatgg ctgcttacaa attttattct caatatgcgt tgaaaaccaa cgacggttcc
     8341 gcttatctgg aaacttatga agatcgagta gccttcaatg cgctttactt tgcgaacggg
     8401 gatgaagaat tagcacttgc tcttgcagac gaaatgatcc atcaacgata tcagccagct
     8461 acaccttctt tcttgaatgc tggacgtaaa cgtcggggcg aattggtgtc ttgtttcttg
     8521 actcaagtca ctgacgatat gaactctatc gggcgctcaa tcaactctgc tttgcaattg
     8581 tcccgtattg gtggcggcgt agggattact ttatcaaatc ttcgtgaagc cggcgctcct
     8641 atcaaaggat atgagggtgc tgcaagtggt gtcgttccag tcatgaaatt attcgaagat
     8701 agtttcagct attctaatca attaggtcag cgtcaaggag ctggcgtggt ttatttgaac
     8761 gttttccatc cagacattat tgctttcctt tcagcaaaaa aagaaaatgc agatgaaaag
     8821 attcgcgtaa aaactctttc tcttggcgtg atcgtacctg ataaatttta tgaattgaca
     8881 cgtaaaaacg aagatatgta tttatttagt ccatatagtg tcgaaaaaga atatggtgtc
     8941 ccattctctt atgtaaatat tacagctgag tatgatcatc tagttgcaaa tccaaacatt
     9001 cgcaaacaaa aaatcaaagc gcgtgatctt gaaaatgaaa tctcaaaatt acaacaagaa
     9061 tctggctatc cttacgtaat caatattgat acagcgaacc gcgagaatcc aatcgatggc
     9121 aagatcatca tgagtaattt atgttcagag attttacaag ttcaaacgcc ttctgtgatt
     9181 aacggcaaac aggaatatga agtattagga acagatatct catgtaactt aggctcgaca
     9241 aatatcgtga atttgatgca aagccctaac tttggtcaat ctgtgcgtac gatgactcgt
     9301 gcattgacat ttattacaga tgcatctgat atcgatgtcg taccaccgat ccaaaacgga
     9361 aataagctga atcatacgat tggattaggt gccatgggct tacacactta tcttgcaaaa
     9421 gaacaaatgg aatatggctc agaggattca ttagacttta caaatattta cttcatgctg
     9481 ttgaattatt ggacacttgt tgaaagcaac aatatcgctc gtgaaagaaa acaagttttc
     9541 cataattttg aaaaatcagc ttatgctgac ggcacttatt tcgaaaaata cgtaactgga
     9601 gaattccagc caaaatcaga aaaagtcaaa gcattgtttg aaggcatttt tatcccaact
     9661 gctgaagatt ggaatgcttt gaaacaagca gtaatgaaag atggactgta tcatcaaaat
     9721 cgcttggcag tagcaccaaa tggctcaatt tcatatatca acgatacgag tgcaagtatt
     9781 catcctatca ctcgcatgat cgaagaacgc caagagaaga aaatcggtaa aatttattac
     9841 ccagcgcctt atctatccaa tgagacgatt ccgtattaca cttctgctta tgatatggac
     9901 atgcgtaaag taatcgatgt ttatgcaacg gcacaacaac acgtcgatca aggcatgagt
     9961 ttgactttat tcatgcgttc tgaaatccca gaaggcattt atgaatggaa gacaacaacg
    10021 aagcaaacga cacgtgattt gaatatttta agacattacg ctttccacaa aggaatcaaa
    10081 tccatttatt acgtacgtac ctttactgat gatgcagaag aaatcggaag caatcaatgt
    10141 gaaagctgcg tgatctaata tcatagatag attcgcttat tcatatttta attaaaggag
    10201 cattctcatg tcagaaacat attatgaagc aataaactgg aatgaaattg aagatatcat
    10261 tgataaatcc acttgggaga agctgacaga gcaattttgg ctagatacac gtatcccact
    10321 ttcaaatgac ctagatgatt ggcgtacatt gtctcaattg gaaaaagata cagtaggaca
    10381 tgtatttggc ggtttgacac tgctagatac tgtccaatct gaaagtggca tggatcagtt
    10441 gcgtaaagat gtccgcactc cccatgaaga agccgttttg aacaatattc aattcatgga
    10501 atccgttcat gccaaaagtt attcttctat ttttagtaca ctgaatacga aaaaagaaat
    10561 cgaagaaatt tttgactgga caaatacgaa tccttatctg caaaaaaaag ctgagcgtat
    10621 caatgaaatc tacaaacacg gtacaccgct agaaaagaaa attgccagcg tctttttaga
    10681 aacattcttg ttctattcag gtttttatac tcctctttat tatttaggaa acaacaaatt
    10741 ggctaacgta gcagagatca tcaaactaat catccgagat gaatctgtac atggaaccta
    10801 catcggttac aaattccaat taggattcaa cgagttacct gaagcagaac aagaaacgtt
    10861 aaaagactgg atgtacaatc ttctttatga gctttacgaa aacgaagaac gttatacaga
    10921 agaattatac gatccaatcg gctggacaga agaagtcaag acattcctac gctataatgc
    10981 caacaaagca ttgatgaacc tagggatgga tccactcttt ccagatactg ccaatgatgt
    11041 caacccaatc gtaatgaacg ggatttcaac aggcacaagc aaccatgact tcttctcaca
    11101 agtcggaaac ggctatttgt taggtcatgt agaagcgatg aaagacgacg attatttgat
    11161 tggattggaa taaggattta ttttaattca aaaatttttc gaaaaataaa aagagtttag
    11221 cgtacagata ttttaaatgt acactgaact cttttttatg cttattcact tgtttcttcc
    11281 agcacttttg gactgtctaa cttttccgaa acatgttcca ttcttctcaa taaatagatt
    11341 ttccggatat ttaatattat cgtcttaatc actgcataga caggtatgca aatcagcatg
    11401 cctaatattc ctgcgacact tcctgctcca atcaatacca agatgatcgt caaaggatgg
    11461 atattcattg atttcccaaa taacaacggc ttgatcaaat ttccatctaa ttgttggatc
    11521 actaaaatcg acaacgccat atacaaagct tgaaatggag agataaacaa accgacaatg
    11581 attgctggta ccgctccaat aaaagggccg acatacggga taatattcgt gattccgcaa
    11641 atgaccgcaa gcaacaagct ataaggctga tcgaatattc tcattgccaa aaagctcatt
    11701 acaccaataa tcaaagcatc taagatcgta ctgctgatat aggcagataa tgtttcgttt
    11761 aattctttta ccgtttggcg taactcttta cgaatcgctt tcggaaagaa gtgagacaac
    11821 gcatcaataa atttgtgccc atccttgaac ataaatagca aaataaatgg aacggtaaat
    11881 aacaaaataa aaaattgcat cataaatgaa atgatttttg agacacttgc agataaactg
    11941 actaatatga tattgaacaa attactaaga gataaatttg ctgatgctaa ttgttcttcc
    12001 aaattgaaat ttttaaactc ttcccgctgc gccaattcat ttaaccattt gcttgtttca
    12061 tctgcataca ttggcaagct ctgggtcaat tgaattgtct gttccattaa ctgaggcact
    12121 acattcatga cagttaaaac aactaaaatg attacgacca aaaacgtcaa taaaaaaccg
    12181 aacactctcg ggatccgatg cttttcgaga aaaacaacga ttggatcgaa catataatag
    12241 ataaaaccag caacaagcaa tggtaaaaaa attgccgaaa gcattttcaa tattggacta
    12301 aaaatattcg gcatttgtaa aataaagtac acgccgatga tcgtgagtac tagctccact
    12361 gtccagaaaa ataattttga ttgtttaaaa cgtgattgca tacatctcct ccaaggtcct
    12421 ttgatacttc tattctatca tttaacaaag agttacgcgc tgagattttc atttgcttaa
    12481 aaaaaacaaa aaaaataaac atattttaat gagtagacga gtacttaaac aaaaacacgg
    12541 tttggagtag tgacaaaaat tatactgcag tccctttttc caaataaacg gtgttcaaaa
    12601 gcttgcatct tcgtaataag cccagccaaa cgaaaaatat aaggaactat ttttatttgg
    12661 ctgggcttat tacttgatgt aggacatcta ttttacaacc tcagatcatt tattgcgcaa
    12721 tactgactaa atgcaacttt gtgatatttt tgtgtacgga caactcctcg atcacatcgg
    12781 cataagtgag cccttttgga agatcaatcg tatagatatt tttgtacact cggtagtcat
    12841 caacgaattt tacgtcaaaa ttcacatctt cgattacgat atttttctct tcaaaataat
    12901 cggtaagaaa atctttggtt tcttttctat ggataaattg gatttctaac tttttagtcg
    12961 taggtacatg gatcacgcgt ttcactactg tgagtgcaat agcaatcccg ataaaactga
    13021 tgatcgcaat ggaatagtaa cccatgccga tggcgatccc taatccagca attgcccata
    13081 aagaagcggc agtcgtaagt cccgtcaccg attgtttcgt gacgatgatc gttcccgcgc
    13141 ctaagaaacc gacaccactc accacttgag caatcagcct tgcttcatct gaacgaatcg
    13201 ttccagcaag ctgtggatac tctaaagcat ctcttaatgc accactagcg atctcaactt
    13261 ggatcaatgc aatgatacat gctcccatac aaactaaaat atgcgtacgc atacctgctg
    13321 gacgattttt atattgtcgt tcaaaaccga ttgctccgcc aaatagcata gcgatcccta
    13381 aacgtaaaat aatctctgga attgtcaata ccatatcatt catctaaaca ccctccagtc
    13441 caatatatca tgtctgaaag gagcagacaa aaaaagtgca atttttacct cttacgaaaa
    13501 aaggactgtg acaaaatttt tgtcatagtc ctttttatga ataaacggtg ttcaatcagc
    13561 tgaccctcgg tattactcaa caaacggcaa aatatgggga gctatttttc ctttgtcttc
    13621 tctaatactc aggtcaaaac gctgatttca caacctcttt agcctactat tgaaaattac
    13681 aacttacttg ctgcttcttc atcgatgatc acaacaacat tgtcgtgatt ttgcaataca
    13741 cttgctggca aatgattcgt tacaggtcct tcaatcatcc ctttgattgc atcagctttg
    13801 ctttcgccaa atgccatcaa gacgatttct ttacttttca taattgaacc gattcccatt
    13861 gaaacagctt ttgttggtac atcttctgct ttatcgaaga atcgtttgtt tgcattgatt
    13921 gttgattctg ttaagtcaac tactgaagtc gttccgtcaa atgaagcacc aggttcattg
    13981 aaaccgatat gtccattttg cccgattcct aagatttgga tatcgattgg atgtgcatcg
    14041 atgatttgat catacttttt gcatgctgct tccagatctt ctgcttttcc atctggtaca
    14101 aacgtttctt tgaatggttt tttatcaaat aattggacat ccatgaaatg acggtagctt
    14161 tgttcatctg aaccgcctag tccaacgtac tcatctaagt ttacagaagt catttcactg
    14221 aaatccaaat cgcttgaagt catttcttta tataaagtaa ctggtgtgct tcctgttgcc
    14281 aatcctaata cttttgcacc tttgttcata ccttctttga tgatttcaaa tgcttttttt
    14341 ccgccttctt cagcgttttt tacacgaatg atttccatag tgattgctcc tttatatttt
    14401 ggtatagccc aattatagca ttcttttttt ctttttacaa taattagtat agaccaaatt
    14461 gcattttttt cattttcttg acaataaata ggaatccgtg ttaggtttca taagatggtt
    14521 caatcaaaca atccatgttg catgaaaacc aagcaagtga ttcactgtgg acgctttatg
    14581 tctgcagttt ttttatgaaa aaggagttat tatgagtata ctgacattaa acaacattac
    14641 ccagcaattt ggcgacaaaa tcctctatga aggtgtcgat ctgcaattaa atgctggcga
    14701 acatcttggg ctgattggac aaaacggagc cggtaaaagt acattgatta aaatcatcac
    14761 tggtgagatc ttaccagatg acggacagat ccattggcaa aaaaatattc acaaaggtta
    14821 cctcgatcaa tatgtggaag tcgatgaaac attaacaatc gaagaatttc taaaaactgc
    14881 gtacgcaaaa gaatacgaaa aggaagaaaa aattgctttt ctttaccaaa catatagcga
    14941 gaccttcgat gattccttgc tggaaaaagc tgggaaactc caaacggaat tagaccaagg
    15001 agtgttttat caaatcgaca ccttagttgc tgagatgagc agcgggttgg ggatcgatgt
    15061 cttaggttta gattcactgt tgaaaaatct aagtggtgga caacgttcaa aggtgatttt
    15121 agcaaaacta cttttagaaa acccagatgt tctgatctta gatgaaccaa ccaatcactt
    15181 agatgatcag catatccaat ggctgacgca gtttcttcag gattttgacg gtgcgtatat
    15241 cgtgatttcc catgaccaag aatttcttga tcagatcacc acccatatcg cagacatcga
    15301 gtttggaaag attactaagt atactggcca tctaaaacaa gcattgaaac aaaaggaaca
    15361 aaatcgcgaa agctatttgc gccaatatca tgctcaacaa aaacaaatcg aaaagaccga
    15421 agcttatatt cgaaaataca aagccggttc ccgatcaacg atggcaaaaa gccgacaaaa
    15481 acaattagat aaaatcgaac ggctgacgcc tccagcttca gcaagtaaag ctgtctttga
    15541 ttttccattc tcgcctattg tcacgacatt agcagtggaa acaactgatt tagtcatcgg
    15601 atatgatcga ccgctacttg cacccatcaa tttgaccatc cgctttggtg aaaatattgc
    15661 cattcgaggc ttcaacggga ttggaaaatc tactttatta aaaacactga tcggtgagat
    15721 tgaaaaaata agtggcgatt tccacttccc agataacaca aaaatcaatt acttttcaca
    15781 agatctgtac tgggaaaatc cattagaaac accactacaa tatttaagca gtcaatttcc
    15841 aaaagcgact atcaaagaat tacgtcgcca actagctaaa gcagggttag ttaatcaatt
    15901 agcaagtgaa cctctcacta cattaagtgg tggggaacaa actaaggtga aacttgcaca
    15961 gttgacaatg aaccaaggta atttactgat tctcgatgaa ccgacgaacc atatcgatca
    16021 agaaacgaaa gaaagcttac aagacgggat acagaaattt ccaggaacag taatcatcgt
    16081 ctctcatgaa caagaatttt atcaagatct tgtgcaacga gtcattgaga ttgaaccaaa
    16141 ataaacgttt gattgaatag ttgaaaaaaa ggagctgtga caaaagtttt gtcacaactc
    16201 cttcttctga ataaatggtg gccaattagc tgaccttcgg tattagctca aaaaacggca
    16261 aaatatgagg agctattttt atttggctat tcttattact tgatgcagaa cagcttttca
    16321 caacctcttt tcgtctattt tttattttcg agatttctct ttcctttaaa aacaactgtt
    16381 tgaaagaaga acgcacatcc ttgaaaaaca agcccgctaa ccgttacacc taaccagcca
    16441 tactgctgcc acattgtcgt ccctagcaat gaaccagccg cacctccaat aaagtacata
    16501 aacataaata ca
//
LOCUS       DAYYGS010000059        16256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000059 DAYYGS010000000
VERSION     DAYYGS010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.059"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..1024)
                     /locus_tag="ACHA44_001913"
     CDS             complement(296..1024)
                     /locus_tag="ACHA44_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905181.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="ACHA44_001914"
     CDS             complement(1002..2576)
                     /locus_tag="ACHA44_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HFM8905182.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="ACHA44_001915"
     CDS             2805..3743
                     /locus_tag="ACHA44_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905183.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="ACHA44_001916"
     CDS             3755..4666
                     /locus_tag="ACHA44_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905184.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="ACHA44_001917"
     CDS             4690..6162
                     /locus_tag="ACHA44_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8905185.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="ACHA44_001918"
     CDS             6213..6824
                     /locus_tag="ACHA44_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HFM8905186.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="ACHA44_001919"
     CDS             6854..8767
                     /locus_tag="ACHA44_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HFM8905187.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="ACHA44_001920"
     CDS             8895..11876
                     /locus_tag="ACHA44_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HFM8905188.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="ACHA44_001921"
     CDS             11873..13534
                     /locus_tag="ACHA44_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-GlcNAcase"
                     /protein_id="HFM8905189.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="ACHA44_001922"
     CDS             13574..14506
                     /locus_tag="ACHA44_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HFM8905190.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
     gene            complement(14898..>16256)
                     /locus_tag="ACHA44_001923"
     CDS             complement(14898..>16256)
                     /locus_tag="ACHA44_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFM8905191.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT         5216 a         2640 c         3240 g         5160 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagttgaaa
    14821 agtaagcgcc ccatagaaca gtacctgtac gtcaaataac ccgacctata gaatagaatc
    14881 gggaatgttt ggcgttttta gttacgagaa aaatgttgtt gtacatcctt gttccaaggc
    14941 aacagttgat ctaaagtttc tgctgaaagt acgggaagat ttggtatctt atcaaataga
    15001 aactctagat actttacagg attcaatccg ttaagttctg ctgttttata tacactcaga
    15061 atgattcctg aagttcgtgc tccttcaaga cttgtggaat gcaaccaatt tttccgtcct
    15121 atcaccagct ctttgatatt tcgctccgca cgattatttg tgagttctaa acgaccatct
    15181 tcaaaaatag gttcaaaacc acttcgtagt ttacacgcat attgaacggc tgcatccaac
    15241 ttggattttg caacaggatc tatcgttgcc atccactcat aaaaagcaat aaataatgga
    15301 cgcagttctt gttgccgaca gcttaatcgc ttctcaggag aaaagtcttt ccatttcttt
    15361 tctaggacaa accatttatc caattgtttg actgcttgag ctgcgggaat atccgtgttt
    15421 ttctttcctt ttggaattgc ttcgaaaaat ttccgccgaa catgcgcgaa acaatagaca
    15481 agtcgtacac tttccacggc attatacccg ctatagccgt cacagtgtaa ataccctgta
    15541 aactccttga gaaaagtttt cggaacatct ccagcacgac tttcgtcgtg gtgataataa
    15601 acgatgggat gctgactgtc ttttcctgta caaaactgcc agtaataggt atctgttttg
    15661 gcactttcaa tcacacgata acttgtttca tccccgtgta gaatctcttc ttgattaaga
    15721 tagactctta atcgttctcc taaagggtct aacgcgtttt gcactgcttt aatatgccaa
    15781 ttggtgatat tatctctcga aatatccaac ccatgtgttt gtttccaata gttctcttgc
    15841 cgataggcag gaactttctg accaaatttc atccggatcg tttccgccac taaactattg
    15901 gaacctaagc tattagaaat caaaggttta ggaacgatcg cttttttaaa agaagaaagt
    15961 ccttcttttt cgcagctttt acaacaatag gtatgttgat aatgggtgag acaatccaac
    16021 atagcctgat ggaaacgaat ttcttctcgt acttttgtcc gtccgatatc tttcatctgt
    16081 gaaccgcaat gttcacaaaa ttgttcttcc aaaggaagga gatgttcttt ctctgttata
    16141 ggtaagtgtt tgattttgtc tgctttcgtt cctacaactc gttgatgtcc ttttactgga
    16201 ataatggtgg cagctgcttc tggaacactt acagaaattt cttcatcact aaaaag
//
LOCUS       DAYYGS010000060        15975 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000060 DAYYGS010000000
VERSION     DAYYGS010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.060"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            216..884
                     /locus_tag="ACHA44_001924"
     CDS             216..884
                     /locus_tag="ACHA44_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFM8905192.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1084..1404)
                     /locus_tag="ACHA44_001925"
     CDS             complement(1084..1404)
                     /locus_tag="ACHA44_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905193.1"
                     /translation="MGDCFCDGRTSQRYQKNEEEARKQPLPKQQMTLQEIDSILTEAF
                     LSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYFMIDHQPILWEDVRYIELKETKK
                     WFVT"
     gene            complement(1441..2823)
                     /locus_tag="ACHA44_001926"
     CDS             complement(1441..2823)
                     /locus_tag="ACHA44_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8905194.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADQANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2979..4166)
                     /locus_tag="ACHA44_001927"
     CDS             complement(2979..4166)
                     /locus_tag="ACHA44_001927"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFM8905195.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4173..5204)
                     /locus_tag="ACHA44_001928"
     CDS             complement(4173..5204)
                     /locus_tag="ACHA44_001928"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFM8905196.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5250..6674)
                     /locus_tag="ACHA44_001929"
     CDS             complement(5250..6674)
                     /locus_tag="ACHA44_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HFM8905197.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            6849..7754
                     /locus_tag="ACHA44_001930"
     CDS             6849..7754
                     /locus_tag="ACHA44_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFM8905198.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7845..8522
                     /locus_tag="ACHA44_001931"
     CDS             7845..8522
                     /locus_tag="ACHA44_001931"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFM8905199.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8519..8689
                     /locus_tag="ACHA44_001932"
     CDS             8519..8689
                     /locus_tag="ACHA44_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905200.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8803..9699
                     /locus_tag="ACHA44_001933"
     CDS             8803..9699
                     /locus_tag="ACHA44_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8905201.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9754..11124)
                     /locus_tag="ACHA44_001934"
     CDS             complement(9754..11124)
                     /locus_tag="ACHA44_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8905202.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11362..12108
                     /locus_tag="ACHA44_001935"
     CDS             11362..12108
                     /locus_tag="ACHA44_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFM8905203.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12105..12944
                     /locus_tag="ACHA44_001936"
     CDS             12105..12944
                     /locus_tag="ACHA44_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8905204.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12945..13894
                     /locus_tag="ACHA44_001937"
                     /pseudo
     CDS             12945..13894
                     /locus_tag="ACHA44_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
     gene            14035..14787
                     /locus_tag="ACHA44_001938"
     CDS             14035..14787
                     /locus_tag="ACHA44_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905205.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14806..15957
                     /gene="queG"
                     /locus_tag="ACHA44_001939"
     CDS             14806..15957
                     /gene="queG"
                     /locus_tag="ACHA44_001939"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFM8905206.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5187 a         2991 c         3221 g         4576 t
ORIGIN      
        1 cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa aaaaggagtg
       61 gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca cagtcctttt
      121 tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga gaaagcatat
      181 ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt acaatgaaac
      241 tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag aaatccatgt
      301 cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc ttagaagaat
      361 caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg ctttgggcag
      421 taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat cgttgggtca
      481 aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag ttgacaaata
      541 accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc ttttttcttg
      601 aaaatgccaa acatgttatc ctagagaatg ccggtcaaat gagtgtattg aaaaaatcag
      661 atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag cataaagtgc
      721 ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa gaaggctatg
      781 cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc tacatactaa
      841 ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac agaggttgtg
      901 aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc tctgatatct
      961 aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc acaaaaattt
     1021 aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt ctgtcacaac
     1081 ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta cgtcttccca
     1141 caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa tgaagtgccc
     1201 ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa tgattactgc
     1261 tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg tcatttgttg
     1321 tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt tgataccgtt gactagttct
     1381 tccatcgcaa aagcagtccc ccattttaat ccaaatggcc gatctcgata ctcgttatag
     1441 tcaatgaatt tttttgctgt ccgtctcatc ttttccacct tctaatccac tcataccacc
     1501 tgcatgcccg ccaacgagag aactacgtgc gatggcgcgt cctccttgca agagactatg
     1561 cgcatggatg attgattgaa agccgtattt ttttctaatc gtatcgacaa caaaatcaac
     1621 tttcgcttca tttatctgct tttcaggatc ttcaaaaagg ttaagctgta aacctgtcgc
     1681 gtatacgagg tcggaacagt tcacgcctac acgccgaata tcctggtagt ggtaatgctg
     1741 gtggaaaatg gtcaataaag catctgcaag atctctgcta gcatttgtcg cttcgatttt
     1801 catttgctgg tgaaacccgg tcttgccatt tggatcaaga tagcctaaag agtatcctac
     1861 ccataagctg actacttgtg tttttgctgc agatcgtctc aagcgagtgc cgacttggtc
     1921 ggccatttct ttgataacga tctcgatttc ttctcgtctt gtataatctc tatctaggat
     1981 ttgactattt ccgatacttt ttgattttgg tgtatattct tgtcccaaaa aacttcgatc
     2041 gatgccccaa gcatgggcat aaagctgagt accgatgacc cctagcttgc ttttcaagtg
     2101 ataataatcc gcgtgagcta aatcataaac agaatgtata cccatttgtt tcagacgttt
     2161 tgctgttcgt ttgccgattc cccaaaagtc agtgatatct gagattttcc ataacttttc
     2221 tggtacatct tcataacgcc attcagcaaa catttgcttt ccgtcttttg agccattgtc
     2281 taaggctagt ttagcaagta gcgggttatc gccaattcct atagtcgtgt agattccggt
     2341 ctgctcaaac acatctttat ggatcagttt agccatgtcg tacgcatttg ttttaccaaa
     2401 gagtgccatg ctatttgtta catcaaggaa gctttcatcg acggaataga catgatggtt
     2461 cgcttggtct gcatattttt tatagataga attgatttct ctgttctttt ccatataata
     2521 ggccatcctt ggggcaacaa tatataaatc acttggatag gggaagggaa gatctctggc
     2581 acggctgata ttggtgatgc cgtaagcttt ttttgctgca ggactggaag ccaagattaa
     2641 tccgctaccc cgtgtttctg tcgaatcgga cggataggac atgactacta atttagttgt
     2701 aaggggatca agatcctttt caacgcactc tacggaggcg taaaaagatt tgcagtcaat
     2761 acataaaata tctcttgaag gttctttttc ataaacaaat accggatttt gattcgccca
     2821 cataacaatc acctcttcaa cacaattaga acacacgttc tctttccgtg tcaataaaaa
     2881 acagcaattt tcaaatacga gcaaaaggct cacatttgga aattgctgag aggttatttt
     2941 tatctatttg tttttagctg agatcgttga tttttttatc aaacatgaac agcttctgga
     3001 tcgatctttg ttgtcccatt tccaatgtgt gcaacataaa attccggagc atagccgaca
     3061 actttcatgt attcatcata gacattgttt ttgaaggcag gtatattttc ttctttgaca
     3121 agagcaatcg cacagccacc aaatccagct cctgtcattc gtgcacctaa gacaccttct
     3181 tgtttttgag cagcatctac tagtgtatct aattcgatcc cagttacttc ataatcatag
     3241 cgaagagagt ggtgagaatc atttaacagt tgaccaaatg cttctaagtt cccagcttct
     3301 aattcagctt ttgccttcaa agtccgctga ttttcagaaa cggcatggcg agctctgcgt
     3361 atcaatgtat catcgccgat ttggtcaata gttgattcaa attcttcttc atctaagtct
     3421 cctaaagaag agataggcaa agttttttgc aaacgtgcca aggcttcttc gcattcgcta
     3481 cggcgttcat tgtattttga atctgccaat tcccgccgct tattcgtatt catgatgacg
     3541 attgcataac catctagttt cactggaacc atttcgtatt tcagcgtatt tgtatctaat
     3601 aagattgcat gatctagctc accgaagccg atggcaaact gatccatgat acctgaattg
     3661 acaccgatga attcattttc tactttttta cctgtttgga ctagttccaa gcgatctgtt
     3721 tttaaatcaa acagatcttc taatacaaca cctactaata attctaatga agcgctactg
     3781 gaaagtcctg caccattagg gattgttcct tccactaata actcaaaccc atgatcgatt
     3841 gagtgtccag cttcttttaa tttaaggacc attcctttta cgtaatttgc ccagtttgct
     3901 tttttatcat attctagctc atctaaagta aaactaatga caccgatatc gtcaaagttt
     3961 gtggaataaa ctaatatttt attatcgtct cgaggtgctg ctacaccttg cgttccataa
     4021 gtgatggaag cggggaagac atggcctcca ttgtaatctg tgtgttcacc aatcaaattg
     4081 atgcgtcctg gtgagaagta ttgtgtagtt tttttaggtc cgaatgtttc ggcaaatttt
     4141 gtgtttaagc ttcttttgat ttccatgatg agttattcct ttcttgtttt gattttgaat
     4201 tctgttactg tttcaaatac tgatccggct tttaaatgaa tcgaaccaaa tgctgagaag
     4261 cgctctgcac ctggagctgt ttgagtctca aatgtgatgc caccgtgatg tgccagtttt
     4321 cggttgcgca tttctggtgt ttctgtgccg aaatttgcag tgaaaataac cacactagaa
     4381 gcatctgtag caatatctac ttggattctt ttatcggggc tagtcaagcg agcggcttct
     4441 ttacccaagc cggtttcttt taagaaaaat ggatgatcga ttccgtcaac aagatttttt
     4501 tgatccaaat cactagcaaa aacggacgct aattttttag gtatttgaaa atcgaaagca
     4561 cttcctgcag cattttcttt tacccctata ggaatactat ctgttcgcaa aggagcataa
     4621 gcttcgctat tgagccaaag ctcatgttga tcgattgact gagaagcatc tcccgtcaag
     4681 ttgaagtaaa catgatttgt cggattgaat aaagtatctt ggtcactggt gccgcgagag
     4741 gtcactcgcc agatattatc ttttgtcaaa gtatagcgga tttccacatc cattgtacct
     4801 ggaaaaccat gttctccgtc gggacttgta gtagtaaaaa tgacagaagc ttcattttcg
     4861 ccattcaaaa tgacataaga ccattttttt aattcaaatc caggagcacc accatgcaga
     4921 ttatgtccag tttttggatc tgtggctaat tggtaagttt ttccattgag agaaaagcgg
     4981 ccattttcga ttctccctgc ggtgcgaccg atagaagcac cgatatacgg atctttttcg
     5041 atatattctt cagcggtgtc aaatcccaaa actaattctc gatcatttga ttttaaactg
     5101 acgattctag cacctaaatc agagatagaa agtgctagct tgtttttgtt ctccaaggtt
     5161 atcaagtgat aaccttggcc aaaatctttt tctgtaatct tcattttcaa gatcctttct
     5221 taatgggaag gaactactgc atcgtgaagt taatcttcca ttgtatcagc tggagccaat
     5281 ttgttttcca atttctcaac gatttgtgca tgttttttct ctgtcaatgt aatcttccaa
     5341 gcaaaaataa tagcggagaa aatcatcaaa gcagcaggag catagaatgc ataagctttg
     5401 aaagtcgtta cgccatgagc agtgatatca gcagcagtag cattaccagt catcccagaa
     5461 gcgatagcga caaaccctac gatcccgtta gacaaagcac cagcgatttt atctagtaaa
     5521 gggcgaattg atagagtaac agcttcgtta cggataccat ttttccattg accatattca
     5581 acagagtctg tgatcgtcat caaagcggat aagaaaatca attgttgagg gaagtagaac
     5641 aatactagcc cgatgataac gattggcaaa cttgaacctg cgatcgtaaa gaaaaagtaa
     5701 gaagaaatca tcattgcgat ccctccaagg taaacgtagc gacgagagat gacgcgtgta
     5761 agcattggga atagtggaac agctactaaa ccaagtaagg cagcaactag accaacgatc
     5821 caaaattcag ttggttggcc aagtacatat ttgaagaagt agaataatac ggcagttgtt
     5881 gctgcattgg caatcgcata agcgatataa gcaagggcaa gccacatcaa ctgatcattt
     5941 tgagcaactg ctttgaacac atctttgatc ccagcttttt cattttgctg acggatcgca
     6001 gtatcgactt cttttgtgcc caaggcagtg ataatcgcag taccgcctga aataacagca
     6061 gcaatcacac cgaaccagaa ccagcctgaa gcgccttcaa tatgtttatg agtaacaaga
     6121 taggtaaaga aagtaacgat agggacaacg actagagtag taccatttgc acctagagaa
     6181 gagccgaaac gagcaaacgt agcaaatttt tcacgttcct ttgtttccgt actcaaagct
     6241 ggaatcattg accaaaaagc gatatcctta aatgaataaa aactatccaa taagatgaaa
     6301 acgattgcaa ataatatgat aaataaagtc gtattatgag tagctaagcc aaagaaattg
     6361 gtaaagattg ctaccaagag tacggaacta acaacgccgc cgataactaa ccatggtttg
     6421 aatttaccgt attttgtacg agtattatcg atgatgccac caatcaaagg atcaaaaata
     6481 atttcaacta agcggatacc tacaactaag cttgtaactg tagcaatcat ttttgcattc
     6541 gttgccttat ctgttcctgc gaacatctgg cttgtcacga ataccataaa ataagtgctt
     6601 aatgtaacat agaatgtgtc gtgtccaaaa gcgcctaaag cgtaggacac acgttggatc
     6661 attttgcttt tcataatctg tattcctctc ctgttcaata tataatacta agtaaaccgg
     6721 ttacaacgaa taacaaagat cttttaatga cttaaactta acaagaaatc gcttcctttt
     6781 taatatattt ctgtgacttt tatttaactt tttgttgcat tatactaaag gagtaaggag
     6841 ggatagacgt ggagcaatcg atttttttaa aaaaggaaca tcaacaagta gaaaaagaat
     6901 tgtattttga tttttgtggt ttctctaaaa cgatgccgtt tcattctttt ggtcctgcta
     6961 ttcgcaatga ttacatcctg catgtcgtga tggaaggcaa aggatcttat catgtgaaaa
     7021 atcaacaatt ccaattgaaa aaaggtgatc tgttcttgat tcgtccagga gactcgacgt
     7081 tttacttatc agatggagaa gatccgtgga tgtattgctg gctttcattt ggtgggagtg
     7141 ctgcagaaga aatcattcgg cagtcgcttt tcagagaaga ccaatacacg atggtctctt
     7201 ctgacataca aaaatatatc gacattattt tagaatgcat gaaagatacg gaagaaacct
     7261 tggaaaatga attacagtta acagcattaa cctaccgttt tttatcagtc cttttaaaag
     7321 atggcggaaa ggtcagcggt ggtgtacaaa aggttcattc ctctttagct gttgaaacag
     7381 tcaaatacat cgaggcacat tatgctgaaa aattgtctgt tgaggaaatc gcccagcatt
     7441 tagcggtcaa tcggagccat ttgtcgcgag tattcaaaaa tcatatgggt accagtatca
     7501 aggaatattt gatcggggta cggatcaatc gaggggcgtt tcttctttct ctgacggatg
     7561 aatccgtgga aagtattgcc tatcaagtag ggttcaatag tctagtcgta tttagccgaa
     7621 tgttcaaaaa gagtaccgga gaaacggcta cgaactatcg taaacgtatg aaaaacgaaa
     7681 attttaagaa tattgctttg gataccttga aaaaacagtt ggaaaaacaa accgcagttt
     7741 cttggacaac ctgattagtc tggttaaaat gacaagcatc ggcaaaagaa acttgtggcg
     7801 gttatgctac actgtaaagc gaagactaaa aaggaggaaa agggatgcgt acacttatca
     7861 tcattagcca tccaacgatc gaagaatctt taaatcagca atttttcaaa gaagcttctg
     7921 ctggtttacc agctacttgg catcatttgg aatcaagtta tccagacgga aaaatagata
     7981 tagagactga actacagctc ttggcccagc atgatcggat cattttccag tttccgtttt
     8041 actggtatag ttctcctgct catttaaaaa tttggcaaga taatgtcttg gaacgagcag
     8101 cacatgtatt aaaagggaaa gaactaggaa tcgtattgac aacaggtgtg gcggaaaaag
     8161 aataccaagc tggaggaaaa gaagaatata cgatttccga atttttgagg ccgtatcagc
     8221 gaatcgcaaa taaattccat atgacctatc ttccgccatt cgttttagca caatttatgt
     8281 atttatccca ggaaaaacgt tgggagaagc taattgctta tcagcagtat ctctcattgg
     8341 aaggcaaacc atcgctgact cagcggattg actggtttat tcaaagagta caggaaaatc
     8401 agaaaatgca ggaagaagaa tctgaaaaac aagcatatat cgtagaagca ctgactgagg
     8461 caaaagagca gatcgaagac ttgaccttta cactgcaaga aatgaaagga acttcattat
     8521 gacaaatgat tggctgatcc aacaattaga tgaattaagc gaaaaatcag aatatcaaga
     8581 ccgtgcgctc ttatatgaaa taaagaaact gatggctgag atagacgaac gaacaatgca
     8641 gctgcaagga gaaatcgacg gacaatcttg gaatcatctc aactggtgaa attttcttat
     8701 aaataacaaa gataaaaatg tgcacagcga caattatttg ctacaataag aatatgaagc
     8761 gtttgataac gctattatac ataaaaattg gtggtgaaaa aaatgtatga tgaaaatgag
     8821 tggggagaaa atgttcggat gacccgaaga agacagcaaa aacaggcagc tcgtaagcgg
     8881 atgatcatga ttggggcagc agttgccgtg tttttgatca tagcaggagg agttgcgggt
     8941 ctatattttc atggaaaaaa ttcgtcagaa gcgaaaagta cgaccataac ggaaacaacg
     9001 acaaagaaaa cagagacgaa acaaacaagt gagtcatcaa ctaaaacaaa agagacacaa
     9061 acttctgaag aaaaaacagc aaatggtttg ccagagcaac ttcaagcaat cgtaaatgat
     9121 tattcgaaaa agatcgaaga aaagacgcct gcattgattg aagaatacca gacagaaatt
     9181 cagggaaatc aagagggaat agctggttta tcagcagttg ctaaccaaaa agcacgtgag
     9241 cttcaagcaa tctccgacga agggatcaga aaattacgtg cagcgtatca ggcagcagaa
     9301 aataaagacg gtgtagactt agatactttg atcaatcaat tatcggctaa ttatacgaat
     9361 catgtcgcta agatttccga tatttatttg cagactagcg cctctcttca agcagagtca
     9421 acaagcacac aagacacaac atcttctgat tctgagacag cagagtcggt agaagcttct
     9481 gaaatgcaag ctcgaacaac acaagacaca acagattcct ctgaaactaa taccaacagc
     9541 gcgacttcac aggctgcgac aaccgtagtt ttggaaggag aaggtccaaa tcaaatagcc
     9601 gaacgaactg gcgtaccagt agaaacgatc ttgtcgctga atgggatgag catggagaat
     9661 tatttcctga ctcccggtga gacgctaaaa ttgaattgat aaaagcaata aaaaagaacg
     9721 aagcttccac taaggtgaag cttcgttctt tttttatttt ccggttcttt tttcgcgtac
     9781 aaagtaatgg atcgtcatgt aaagcaagaa aacgagtcca ataatcaatg agattctcgt
     9841 ttcaggattg ataaacatga aaatcaatac gatagctaaa aaagcaagtg tcacataatt
     9901 cgtataagga gatagtggca ttttaaaagg atgatcatgc attttatctg cgtgcttctt
     9961 tttgaagcga atctcgctga tcaaaatcac tacccaagga accataccag gcaaaatact
    10021 tgagctgtat acgaaaacga aaatattttc tgactggttg atgaacattg gtagaagata
    10081 atttaagatc agaccaatta agattccacc tgagattgct acgacaggat aaaaaggcgc
    10141 accgtgacga gatagtttca gaaatttttt agacatcttt ccatttatgg caagtgtata
    10201 gaccatacga cttgcgctga aaatccctga attgcaacct gataatgccg ctgtaatcac
    10261 gacaaaatta atcagtcctg cagcaaatgt aatgccgact ttagaaaaag tttgtacaaa
    10321 aggtgaacca atctcactca attgattcca cggatagata ctcacaatga taaaaatagc
    10381 accaatataa aaaatcaaga ttcgaccgat agtggactgg acagcttcaa tgatcgtctt
    10441 tttaggattc tctgcttcac cagcagtcat tccgatcagt tcgattcctt gataggaagc
    10501 aacaacgatc gataatgcaa agaaaaatcc tttgacgcct ccagtgaaga atccaccatt
    10561 tttccaaaga tttgaaatcc ctactgcctg accatgatta ccaaatccaa ataagattac
    10621 cccaagacct gcaatgatca tcaaaatgat cgttatgact ttgatgatcg aaaaccaaaa
    10681 ttctaattcg ccaaacgcct tgacagaaat aagatttgcc agcataagaa acaagacgac
    10741 aatgattccg ggaataattg ccggcaaatc aggccaccaa taattcatat aagatcctac
    10801 agcaatcact tcacttattc ctacgacaat atattgaaaa atattactcc aagcggtcaa
    10861 gtagccagcc acaggatgga tgtactcgct cgcgtaattt gcaaaagaac cagtcgaagg
    10921 atcaacgtaa agcatttcgc ctaaagcacg cataatcaaa tacaaaaaga atcctgcaat
    10981 cgcataggct cccatcactg agggccctgt ccaacgtatg gttgaggagg cacccataaa
    11041 cagaccgaca ccgatcgttc cacctagtgc gatcatttgg atatgacggg cactaagact
    11101 tcgagttaac ttttcttctt ccatacaaaa actcctttcg ttgttgagtc aacaagaaaa
    11161 aataatcaaa cgtttcaaca catgtttttc attattaggt tagccttatt gaactcttga
    11221 tgtgattcta cagaaaattt tcataaaatg cgaggaaaat tcgaaaaaaa ctttttcatc
    11281 attctacttg aaaatgcttt ccattttagt taaactatat ttaaataaaa agttaggtaa
    11341 acctaaaaag gagtgtaaga tttggaacgt acggccattg taattcaaga cctttcagcc
    11401 gcttatcaag ggaaaactgt tttgaaaact atcaacttga cgataaatcc acaaaaaatt
    11461 accggtatta tcggaccaaa cggtgcagga aaatcaacat ttatgaaatc tttgctagag
    11521 ttgataccag ctactactgg taaagtcacc ttttctggac agtcggtaaa ctcagtccgt
    11581 aaaaaaatag cctacgtgga acagcgaagt gaattggatc tttcctttcc aattgatgta
    11641 ttaggcgttg tacttttagg aacatatcca tctttacgaa ttggacaaag acctgggaaa
    11701 aaagaaaaag aacgtgcaag acaagctttg aaaaaagtag ggatggaaga atatgccaaa
    11761 agacagatca gcgaactatc gggtggacag cttcagagag tttttattgc aagagctcta
    11821 gcccaaggag cagaatggat ctttttagat gaaccattcg tagggattga tgcgttaagt
    11881 gaacgaaaga tctttgacat cttgcaggaa ttgaagaatt caggaaaaac gattttgatc
    11941 gtccatcatg atcttcataa agtagaagaa tatttcgatg aggttattct tttaaataaa
    12001 cagctgatcg cttccggtcc agtacaagaa gtctttacat cgaagaacct tcaattggcc
    12061 tatggtgaga tcatcagaca tttagtaaaa ggaggagaga aaaaatgatc caatccttta
    12121 ttgatggatt aatgaattat caattcctgc aatacgcttt ggtcacttcc atgcttgtag
    12181 gacttgcttc tggagtcatc ggttctttta ttattttacg agggatgtct ttgatgggag
    12241 acgctatctc tcacgcagtt cttccaggtg tagccatttc ttatatgttt ggttttagtt
    12301 acatctttgg agccactgct tttgggatgc taacagcagc agtgattggt ttcgtgactc
    12361 agcggagccg attgaaaaat gatacagcga ttggaattgt ctttagctcc ttttttgcat
    12421 tagggattat cttgatttca tatgcccaga gtgcgacaga tttgtatcat attttatttg
    12481 ggaatgtctt agctgttagg gaaagcgatt tgttgctgac agcactcgtt agtggaatcg
    12541 tcttgatatt tgtctttttc ttttataaag aattgaagat cacatcattc gatccgacaa
    12601 tggcaaaagc ttatgggctg aacacatcgc tgatccatta tcttttgatg ttctttttga
    12661 cattagtggc tgtagtcagt ttacagacag taggaacaat cttggtgatt gccatgttga
    12721 tcacaccagc cgccacggct tacttgctaa cgaaccattt actgaaaatg atcattacag
    12781 ctgcaggaat cggtatgcta agtgcagttg tcggtgtgtt tttcagttat agttacaatt
    12841 ggccatcagg agctacgatc gtgttagcat gtaccgcatt ttttatcctt gcttttttat
    12901 tttctccaac aaaaggaatt ttatttcaga aaggaagcag atagatgaaa acaaaaaaat
    12961 cactcttttt aatcttggca gttagtttct tggtattagc cggttgcgga aagcaggctt
    13021 ctgaccaagc ggatgaagga agtaaagaaa agttgtcagt cgtggctacc aattcgatct
    13081 tggcggacat ggcaaaagag gtcggaacag atcaaataga tatccacagt atcgtaccag
    13141 tcggaacaga tccgcatgaa tatgaagtac taccagaaga catcaaaaag gcaagcgatg
    13201 cagatgttat tttatacaac ggtttgaatc ttgaaacagg taacagctgg ttcgataact
    13261 tgatggaaac ggctaaaaaa gaagaaggaa aagattattt tgcagttagc aaaaatgtag
    13321 aacctcttta tttaactagc ggtgaagaac atacaaaagc agatccccat gcttggctag
    13381 acctatctaa cggaataaaa tatgtggagg aaatcgcacg tatattctct gaaaaagatg
    13441 cagaaaatgc gacactctat aaaaaaatgc agaagcatat gtggaaaaac taaaagaatt
    13501 agatacacaa gcgaaggaaa gttttgcttc tatcgaagag aacaaaaaat tattagtaac
    13561 aagtgaaggg gcattcaagt atttttcacg agcatatgat ttgccagcag cttatatatg
    13621 ggagatcaat acagaaagtc aaggtacgcc tgatcaaatg aaagcgatca ttgatcagat
    13681 aagagcaaaa gaagtaccgg ttttattcgt ggaaaccagt gtagactcaa gaagcatgga
    13741 acgggtagca aaagaaacgg gtttgaaaat ctatgataaa ctgttcactg attccatagc
    13801 aaaagaagga gaacaaggag attcttacta tcagatgatg aaatggaata ttgaaacgat
    13861 ccatgaaggt ttgtcacaaa caaaagaaag ctgaaaagtg caaggtctag cttgaggagc
    13921 agaccttgca ttttttataa gagatatgaa aaaaaattat gctgtttttc gtcgtacaga
    13981 tacctttttt gaagaaattc cgttatagtt agagtaagga aggaaggtgt ggctatgaat
    14041 tttacacgta aagataaata tacgattaaa gaagaagtct ttcgattgat cagccagcag
    14101 gaaaataccg acaaggatca agaccttcaa tttcttgaag cagaattagc taaattattc
    14161 atagcaaatc aaaatgagac aacagaacca gatgaagagc tgtacatagg caaatggttg
    14221 tttgatgaat ttcagccttt agatgaaaat accggtatcg gttattttgt gttcctttct
    14281 ccagtcggct tcacacctgg cgtattagtt aaacaaaacg acgaaataac cgtcaaagca
    14341 tggcctgcat tattttcagt catcaaagca gaaggagtaa tcaaaaagat ccagtcggcc
    14401 tattataaaa gtcaatttat tgccaacagc aaattaatga atatgtttta taatcgtcta
    14461 gtagaaattg ctaggcctgg gaaaatcccg caaacattca cgtacacaca gcaaaaaaat
    14521 ctctatgaag aagtattgta ttcccgcgat ctgtctgagt tttcgccatg ggaagaggta
    14581 aggctccatg aactgttaag tacaaaacca gtttcattga atttttttaa tgcattagat
    14641 aacaaaagaa aattaatttc aacaaattat tgggaagtat ttgctgcaag aaaaaaatgg
    14701 atggaagaaa agaagattgc cgatcagcag atccagtttg tccagctgaa aaaagaaaaa
    14761 ctatattatg ggttatacag ccgttaacag caagcgagga agaccatgac attaaaagaa
    14821 gaaatcatac aagaaagcaa acgattagga attgacaaaa tcgggttcac cacggcggaa
    14881 ccttttgatt cattaaaaga atcgttggaa gaacaaaaag cagctggcca tacttctggc
    14941 tttgaacatc caaatatcga tgagcgtctt tatccagaaa tgacatttga tcagccgaaa
    15001 tcgatcattt ccattgcact tgcgtatcct actaggatcc atgaagaagt gcctagggat
    15061 gaaaaacgcg ggcagtttgc tcgtgcttct tgggggatcg attaccatga tatactcaga
    15121 gaccgtttga accgattgat cgaattcatc caaacaagag cagaaaaatg gcaaaaagaa
    15181 gaggaatggc gtctagctcc gcaagtagat actggagaac tgatcgatgt cgctgtagca
    15241 cagcgagctg gtttaggatt tattggacga aacggattgt tgatcactga agagttcggt
    15301 tcatttgtct atcttggtga gatcgttact aatattgtat ttgagcctga taaaccagga
    15361 gaatttggtt gcggtgactg tatgcgctgc gtgactgctt gtccgacaaa agcattgctt
    15421 ggtgacggaa gaatgaatgc ccagcgatgt ttgtcttatc aaactcagac aaaagggatg
    15481 atgccggaag aatatcgtaa aaaaatgcga aatgtcattt atggatgtga tatctgccag
    15541 ttagtctgcc cttacaatca aggaaaagat tttcattttc atgaagagat ggaaccaaaa
    15601 gtagacgaaa tatatccaaa attaaagcca atgctttcga tgtctaataa agacttcaaa
    15661 cagcaatttg ggcatttagc tggttcatgg cgtgggaaga aacctttgca gagaaacgca
    15721 ttgattgctt tagctaatct aggtgatcga acggcattgc ctgagatttc agaatgttta
    15781 acagatgtcc gccctgtcat cagaggaaca gcagcttggg ctattggacg tctaggaaca
    15841 aaagaaccgg aaaaatggct tgatgtatta gatgaaatat tagctaaaga aacagaagaa
    15901 gtcgttataa acgaattgga acatgcccag aaattattgc ggagaaagtt aaaataaaaa
    15961 aatagttaac attat
//
LOCUS       DAYYGS010000061        15238 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000061 DAYYGS010000000
VERSION     DAYYGS010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.061"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1136)
                     /locus_tag="ACHA44_001940"
     CDS             complement(93..1136)
                     /locus_tag="ACHA44_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HFM8905207.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(1513..2478)
                     /locus_tag="ACHA44_001941"
     CDS             complement(1513..2478)
                     /locus_tag="ACHA44_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HFM8905208.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            2712..3557
                     /locus_tag="ACHA44_001942"
     CDS             2712..3557
                     /locus_tag="ACHA44_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFM8905209.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            3748..4935
                     /locus_tag="ACHA44_001943"
     CDS             3748..4935
                     /locus_tag="ACHA44_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFM8905210.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            4941..5297
                     /locus_tag="ACHA44_001944"
     CDS             4941..5297
                     /locus_tag="ACHA44_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HFM8905211.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            5299..5637
                     /locus_tag="ACHA44_001945"
     CDS             5299..5637
                     /locus_tag="ACHA44_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HFM8905212.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    5713..5969
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6032..7066
                     /locus_tag="ACHA44_001946"
     CDS             6032..7066
                     /locus_tag="ACHA44_001946"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFM8905213.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            7059..7745
                     /locus_tag="ACHA44_001947"
     CDS             7059..7745
                     /locus_tag="ACHA44_001947"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFM8905214.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            7769..8617
                     /locus_tag="ACHA44_001948"
     CDS             7769..8617
                     /locus_tag="ACHA44_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFM8905215.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEEKPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA44_001949"
     CDS             8814..9587
                     /gene="sufC"
                     /locus_tag="ACHA44_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFM8905216.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA44_001950"
     CDS             9600..10886
                     /gene="sufD"
                     /locus_tag="ACHA44_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFM8905217.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            10883..12118
                     /locus_tag="ACHA44_001951"
     CDS             10883..12118
                     /locus_tag="ACHA44_001951"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFM8905218.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYFEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA44_001952"
     CDS             12105..12575
                     /gene="sufU"
                     /locus_tag="ACHA44_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HFM8905219.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA44_001953"
     CDS             12580..13971
                     /gene="sufB"
                     /locus_tag="ACHA44_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFM8905220.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYEPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(14069..15211)
                     /locus_tag="ACHA44_001954"
     CDS             complement(14069..15211)
                     /locus_tag="ACHA44_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905221.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILS
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         4980 a         2844 c         3252 g         4162 t
ORIGIN      
        1 gttgttgtta gaaggatatc tgcaaaatta aaaacagttg tgattcggac attgttccat
       61 gcgagaatca caactgtttt ttcatgttca ttctaaactt tgatcagcca aataaatagt
      121 aaagccatca ctgtttggag aagcccgaca gctaatccat aagataagaa gcgtgtacct
      181 tctttcatga attttgcaaa gtctaaacgc agaccaattg ccgctaaagc agtgatctca
      241 aaccagctgc tgataaaatg tgccgattgg tcaataacta caggtaaaga aaacaagctg
      301 ttgaccaaac aaaagatcac aaaccctaat acataccaag gaagcggtaa ttttcgtttt
      361 ttctgcgtat cagttggcgt ttcaaacaga gctggttgtt tcttgtgcat gaatttttca
      421 aacagataga caacaacaac gagcatcata atacgtagaa tcttaaatag catggcgaat
      481 tcgacaacag aattgtcaag taagcttgcc ccagcgacta cttgaccaac agattggagt
      541 gtaccaccca aaagtgcact tttttcaatg agatttccac caaacaactg taatccaaga
      601 aaagggagag tcaacatcat cacagtaccc agtagattga ctagtgtgat gatctgtcct
      661 ttttcttcct ctttcgcatg gatggaaggg gcgattgaag caatagcaga agaaccacaa
      721 acagcgtttc ctccagccat catcaaagaa gaagtatcgc tgaattgcag ttttttacca
      781 atcaagtaag caccgatgat agtgactgac atttgcagta acacaaaaac aattcctttt
      841 acaccaattt gagcaatcgt ttgagctgtg acagttgcac ctagtaaaaa aacagagaat
      901 tctaatagtt tgctttcagc aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa
      961 gtatttccta ataaaatacc caataaaata gcaatagtcg ctccacctaa ctgtggcaga
     1021 taaatagcaa gaagcctgct gaaggcagcg atggcaaaag aaagcagtaa accgggtaag
     1081 atttcgcgga ccgctgaatc ctttacagat tttattggat gtgacaatga tttcatttga
     1141 tcggttcctt tcgtcaataa atactaaaag catactcgaa catagtagca ctaaaccagt
     1201 attttgacaa aagaacaaga ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata
     1261 aacggtgttc cagaagtagc ttctacggaa ataagccgaa atttcacaaa aatttgagaa
     1321 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctttta cgacctcttg
     1381 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccagtac aaaaacatga
     1441 acagctgttt ttgtttggcc gttcttatta cttgatgcag aacagctttt tcacaaccta
     1501 ttgaatcgat tattaagcga tttttttaga aacaaatttt ccatgtttgc tatgttcttt
     1561 aattaagaaa cgtacgtaat gagcaagaac gtagaagaca actacacttg caaacagaca
     1621 ttccgcaact tttaagaaat ccaatacgct actatagata cctaaactgt tgaagtaatg
     1681 aacagtagta aatatagctg ttgcagagat aacaagcggg aatgtaaagg ctgcatagct
     1741 tggataaaaa cgaagcttga acaaacgtgg aagcatagac agcacaacga aatacaatac
     1801 ttgagataaa gcgaacaatg ctacgacaag ataaaaatta ggttcagcaa agtttttaag
     1861 ataacctgtt aggcaaagag aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg
     1921 atgagccata tttcttacga aaaagacacg tttgatgatg atcggcaata agaacgcata
     1981 gcagatcaat gaaatccaaa agaaaatttt tccaataagt gggaagaagt tcccggcagt
     2041 tacagtgatg accccaaatc caacgtaaat gataaaccag cttggataaa ttgcttcaat
     2101 tgaaagatca gctttgataa catggtgata attgaaataa aagacaagga tcacttggaa
     2161 gataacagcg cacagccaaa tgaatttgac aattggtgca atactttcat atgaaacaaa
     2221 ataagtacaa ataaccatga gtgacatcgt aaaagttggt gaaacggagg cgatgattgg
     2281 atcatgcaac gtttgtttcg tgtgttcgaa taagataaac aatttaacaa aaatgaatat
     2341 cataagaatc atagagatac cgccagttag attgcctaaa gcaacatggt gatagtcttt
     2401 aaacagattt ccaagggaag ctaacccaag gatcaacccg cagatcggga tcggaataat
     2461 tttcaaaaag ttcttcatat ataatattct cctttttatt taatagattt tagtaccaag
     2521 atgaacgaat acattgtaaa atgaatagga atgtatgatt acgagcaagc agacagctga
     2581 ttgtcttcat ctaagttact agttggatca gcaactgttt tgttgtacat ttctattata
     2641 gatggttttt tgtatattta aatcaaatag tttttatagt aattatcata aaaagttatg
     2701 gaagtggtgc catgtttaag ttattaacga cttttcgagc agtatatgaa acaagaaatt
     2761 tttccagagc agcagaagta ctgtttttat ctcaaccagc agtctcgaat caaatcaaac
     2821 agttggagcg ggaattgaat atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc
     2881 caactaagca agccgactta ttatatgaac ggacactggt tttgttagaa gaatgggaag
     2941 aaactcagca aaaattgctc aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt
     3001 cccacacctt tgctgtctat cttttgcctc agttgatgaa acatctgatc aaaaggtttc
     3061 ctaaagtgca ttttgagatc gaaatcacga attctcatga atcattagaa caagtagcga
     3121 aacatgaatc cgattttggt tttatcgaaa agccattaga gacttccggc gtacaaagat
     3181 actctttgat ggaagaccag ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg
     3241 taagagaagc aaactctggt gtttttcatt acacgcagcg ttattttgaa gagatgaata
     3301 tccaagataa gaagatgatc gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag
     3361 gaatcggtcg ctcgattttg tctaagcgct ctgttccaga aaatgtgcca atgactccgt
     3421 tgaatttcca tcgatatttt tatttcatcc aacgaaccaa tctggtttct gatcagctgc
     3481 tagagattgc cgaagagatc cttcgataca atgaaagcac agaaacttct cacagcgagg
     3541 agaatctcag ctggtagata attgcatgta tcaaaaaaag ttctatgata cactaaagat
     3601 aagcagtagg cttttgtact gcatttgctc aagcaactgc acaaacggaa agttaatttt
     3661 tcccaaatgt ctatggaaag actttccatt atctaactgg gccgctataa taatcagcag
     3721 tatggttttt aagaaaaggg atgaaacatg gaaaaaaaat taaaagcttc gaacgataat
     3781 cgtattgatt atggcgtgat tttaccagtc tttctgttat gtttgattgg tctgctctca
     3841 ttatatgtag cacttagcca tgatccgaac aatccagcag tcttgaaagg cgtagggatg
     3901 caagttgtct ggtatttagt tggagcggca gcaatcgttg tcatcatgca tttgaattcc
     3961 aaatggatct ggaaactgac tccttacctt tatggtttgg ggttagtggt aatgggactc
     4021 ttgctcaaat tctatgatca gaatttagct gcttcaacag ggtctaaaaa ctggtttcga
     4081 ttcggtgcgt ttactttcca gccagcagag ttgatgaaga ttgcgtacat cttgatgatg
     4141 gcactgatcg taactaaaca taatacgcaa gtaaaagaac ggacgatgaa atcggacttt
     4201 tggctgattg ggaagctctt gatcgtgaca ataccggttt tagcattgat catggcccaa
     4261 gatgactttg gaacgatgct tgtctttctt gcgatctttg gcgggatttt cctgatgtct
     4321 ggaatctctt ggcgcatcat cgcacctgta gtaatcttgg cagtagtcgt tggagcagga
     4381 acgatcttct tggttacgac tgaaggcgga agagacttgc tttataaagt tggattcaaa
     4441 tcttatcagt ttgcacgtat tgattcatgg ctggaccctt tccatgatac gtcaggaatg
     4501 agttatcagc cggcccaagg acttttggca attggtaccg gtggcttgtt tggtaaagga
     4561 ttcaacgtaa gcaatatcta tgtgcctgtt cgtgagtccg atatgatttt taccgttatc
     4621 ggtgaaaact tcggctttat cggtggtgca ttcgtcattt tcttgtattt tattttaatc
     4681 tatcgtatga ttcgggtgtg tttcgatacg aataatgaat tttacgccta tatcgcaagc
     4741 ggtttgatca tgatgctgct gttccatgtc tttgagaata ttggagcaaa tatcggatta
     4801 cttcctttaa cagggatccc attgcctttc attagtcagg gaggatcctc gattttaggg
     4861 aacatgatcg gtatcgggtt gatattatcg atgagatacc aaaatgaagc acctgcaaga
     4921 tcttcaagga ggtaagaaaa ttgtacacct tttactggta cccgaaatgt tcaacatgca
     4981 aaaaagccaa agcgtggtta gaccagcatg gcgttagtta tcaaaccgtt gatatgatca
     5041 aagaagtacc gaaggcctca acgattgaga cttggctaga aacgagtcaa gtaccaatcc
     5101 gtcgcctgtt caacacaagc gggatgaaat atcgggaact tggactgaaa gatcaagtag
     5161 gcgagtttac ggcttcaaaa gcgagccaag tcttatcgac agatggtatg ctgatcaagc
     5221 gaccactgat cttacgagag gaccagcttg ttgcaattgg attcaatgaa aaaatttatg
     5281 aaggagaact gatataaaat ggcaggcaag catttgaaga aaaaggacaa cctttggatc
     5341 ttatttaatg gaaaagaata ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga
     5401 gacatcacgt ttgccaatct gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg
     5461 atcgaagttg aagcggaaaa agcggtcaat gaatttgcca gcccgctaac gggtacagtt
     5521 tcttctgtta atgataaaat cggtgaagat atcaatatct tgaatgattc agatgaaatg
     5581 aatgcgtgga tcttaagttt caaagatgtt gatccaaaag aatttgacga attataaaat
     5641 cagaaaatca cttgaaaaaa gttgacactg attcttccag ttgctataat cataagcaat
     5701 cctatatgaa acgagaaagc tttgaaaaga agagtacatg tcacggacgt ttgaagagag
     5761 ccctgtttgc tgaaaatggg taacgaaaat gagatggaag atggtcttag agcagagtaa
     5821 gcgagcgtaa gcaaacttac tacgggagtg cccgttacag cgcgacagta tacggaaaga
     5881 agctaagtcc aagtactgga agaggctgtt attgcgaagt aacggtaaat caaggtggta
     5941 acacggaagt tcaagctttc gtccttttgc ttaaggcaag agggcgaagg cttttttgtt
     6001 tgaaaaatta taagagaaag atgtgaatgg catgtcatta atccaactaa cgaatgtcaa
     6061 aaagacattc aatgggaaaa acggaaacat ccaagccgtc aatgacgttt cactaaacgt
     6121 tgaaaaaggg gatatttatg gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt
     6181 ccgtctcctg aatggacttg agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga
     6241 tattacaaaa ttaaaaaaca aagaattacg tacattccga aagaaaatcg gtatgatctt
     6301 ccagcatttc aacctgttat ggtcaagaac tgtacttgaa aatatccagc tgccgctgga
     6361 attagctggc gtaccaaaga gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt
     6421 cgggttagaa ggacgcggac aagcatatcc ttcacaactt tccggcggac agaaacaacg
     6481 cgtcggtatc gcacgagcat tggcaaatga tcctgagatc ctgctttgtg atgaggctac
     6541 tagcgcattg gacccgcaaa caacagaaga agtattagac ttattgttag cgatcaataa
     6601 gaaattgaat ttgaccatcg tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg
     6661 caacaaagtc gctgtgatgg agttggggaa agtcgtagaa gaaggagacg tattgactgt
     6721 cttcagacaa ccgaaaaaag aagtaaccaa acgctttgta caacaagaca tcgaaccaga
     6781 agctgattcg acagaactat tagctgaact catcaaagaa aatccaagcg gccgtttggc
     6841 aactttgacc tttagcgaaa gaaatgccaa cgagccagtc atttcccaag cgattcgacg
     6901 ctataacatc gatgtcaacg ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt
     6961 tggttcattg actgtcttga tgactggtga aaccaaagaa ttagatcaag cagaagtgtt
     7021 catcaaagaa caaggagtag gaattgaggt gatccatcgt ggataaaagc ttttcagaaa
     7081 catatctaga ttttaaacaa atcaacccgg aaacgatgaa tcaggctatc cttgatacac
     7141 tatatatgac attaatctca atggtcttag tgacgatcat cggattagtt ttgggattga
     7201 tcctttactc tattggacgc aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat
     7261 cgatcatcag taatattttc cggtcaacgc catttatgat cctgatggtt ttgatcattc
     7321 catttactaa agcaatcgta ggcactatgc taggtgcgca agcagccatt ccagctcttg
     7381 ttttatccgc agcaccattt tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc
     7441 ctggtgtact ggaagcagca gatgcaatgg gagcaagtta ctgggaaatc atctggaagg
     7501 tcttgatacc tgaaagtatt cccgcattga tttctggatt aacagttaca acgatctcga
     7561 tgatcggatt tactgcgatg gccggcgcga tcggcgcagg tggacttgga ggtcttgctt
     7621 ggcaggaagg ctaccagcgt ggtaatctaa cagtgacatt cgttgcgact ttaattattt
     7681 tactaatcgt atttatcgtt caaggaatcg gtgatttctt aactaagaaa acagacaaaa
     7741 gataatagga tataggggga tgtaggatat gaaaaagaaa atttttggat ttgcagcagc
     7801 gcttttgctg actgtaggat tagcagcatg cggtaacaac agcgacagca aagattcagc
     7861 aaataaagca gacgacacga cattgaaagt cggcgcttca ccgacaccgc acgcagagat
     7921 tttggaacat gtgaaaccat tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt
     7981 cgatgactat gtattaccga accaatcttt ggaagaaggc gacattgatg ccaattactt
     8041 ccagcatatt ccttatttga acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc
     8101 cggtgctgtc cacatcgaac caatgggctt gtactctaaa aaagtcaaag atgtttccga
     8161 attaaaagat ggtgcaacaa tcattacttc aaattctgaa tcagactggg gacgcatcat
     8221 cacaattcta caagatgctg gcttagtcaa agtcaaagat ggagtagatc tggaaacagc
     8281 aacattcgat gatattgaag aaaaacctaa gaacttgaag ttcaaccata cgatcgaccc
     8341 atcactttta gcttctacgt accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa
     8401 cttcgcttac ggcgcagggt tgaatccatt agaagatgcc gtattgcttg aaaaggacaa
     8461 ctctccttac gtgaatatta ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa
     8521 aaaactgatt gaagtgcttc accgcaaaga agtccaagaa tggatcacag acaaatgggg
     8581 cggctctgtt aaaccagtaa aggctgatgc aaaataaggt atggaacaaa aaagatgaga
     8641 aataagtctg taatggctta tttctcatct tttttatacc tattctaatt tagaaatcaa
     8701 aattgtttaa taataaataa aagatatttg attttagtaa ggaaggtcat tgcgcttcat
     8761 tctcaattag tctaaaatga gtgagaacga aagagaattt gggggtataa tgcatgtctg
     8821 tcttagaaat aaaaaatcta catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa
     8881 acctaacaat gaaaaccgga gaaatccatg ccatcatggg acctaacggc acaggaaaat
     8941 ctaccttatc tgccgctatc atgggcaatc caaactatga agtaacagaa ggcgaaattt
     9001 tgtttgacgg gaaaaatgtt ttagacttgg aagtagatga acgtgcgcga cttggcttat
     9061 ttttagctat gcaatatcca agtgagatac caggtatcac gaatgcggaa ttcatgcgtg
     9121 cagcgatcaa tgcgaaacgt gatgaaaacg acaaaatttc tgttatggca tttttgaaaa
     9181 agttagatga aaaaatggct ttactaaata tgccagaaga aatggctgaa cgttatctga
     9241 acgaaggttt ttctggcgga gaaaagaaac ggaatgagat cctgcaattg ttgatgttag
     9301 aacctacatt tgcaatttta gacgaaatcg attcaggtct ggatatcgat gctttgaaag
     9361 tcgttgctaa aggagtaaat gaaatgcgcg gagacaattt cggtgcattg atcatcaccc
     9421 actatcaacg attattgaac tatatcacac ctgatgtcgt tcacatcatg atggaaggcc
     9481 gagtagtcat gacagggaat gctgacctag ctaaacgtct ggaagcagaa ggctacgctg
     9541 gtatcagtaa agaattagga atcgactata aagaagaaga agcgtaaggg ggagcaagaa
     9601 tgaaagaaaa aaactggctc gattatcttg acgcggtaaa tgatttttct ttaagtaaag
     9661 gcgaacctga ttggatgaga accttccgtc aagacgcact agcgaaagca gatgaactgc
     9721 cattgccgca tatcgatcgg gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa
     9781 cacaaaccat cagtgaaaca ggcacgattc cggcatttga tgcaatgaaa gacaatcctg
     9841 tgcttgtcca acaaggttca tggacgattt ttgaacaact gcctgtggaa ttagcagaaa
     9901 aaggagtcat ttttaccgac ttgttcactg caatgatcga atatccagaa ctagtccaag
     9961 aatattatat gaaaaaagca gtaaacatga acgaagacca attgactgca ctccatgtag
    10021 cttttatgaa cagcggtatt ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac
    10081 tcgagtcctt attcattcaa gatggcgcat ccgatgagca cttcttcaag catgtgttga
    10141 tcgtggcaga tgaacatagt gaattttctt atctggaacg tttccagaca accaaagaac
    10201 aagtagcaaa atcatcaggt aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa
    10261 tcaaatattc agccgtagat caattaggag aaaatattac ttcttatatg aatcgccgtg
    10321 ggcacatttt acgagatgct tctgtcgact gggcaattgg tgtcatgaat gacggtcatg
    10381 tgatcgctga ctttgactca gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg
    10441 tggcaatcag ctcgggcaga caaatccaag gaatcgatac acgagtaacc aataaagccc
    10501 ctcatacgat cgggcatatt ttgcaacacg gagtgatccg agaaaaagga acattgacct
    10561 ttaatggtat cggccacatc ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc
    10621 gtgtcttgat gctttctgat aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg
    10681 aaaacgaagt aactgctgga catgctgcca gtgtaggacg agttgatcca gaagaaatgt
    10741 actatctgat gagtagagga ttgcataaag aagaagcaga gcgcttagtg attcgaggat
    10801 tcctcggttc tgtcttaaca gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag
    10861 taatcgaagg gaagttgaat ggatgatcga tgctaaaaag ctacgcaatg attttcctat
    10921 cttatttcaa gacgtcaatg atgaaccttt agtttatctg gataatgcag cgacgaccca
    10981 aaagccaaaa caagtactcc aggcattagc gtactattac gaacacgata acgccaatgt
    11041 tcatcgcggt gtccatacgt tagcagaacg cgcgacccat gattatgaac aagcaagaga
    11101 aaaagtacgg gcgttcatcc atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac
    11161 gacgacaagc ttgaattggg tagccagaac cttcggcgac cgctatatca aagcaggaga
    11221 cgagatagtg ctctcttaca tggaacatca ttctaatgtt atcccgtggc agcagttggc
    11281 aaaacgaaca ggtgctgtct tgaaatatat cgaactaaca gaagaaggct tcctggatat
    11341 ggaggatgca gcgaaaaaga ttacggacaa aaccaaaatc gtctccattg ctcatgtatc
    11401 caatgtctta ggtgtcttca atcctgttca agaattagct gaacttgtcc atgaaaacgg
    11461 cggggtcatc gtagtagatg gtgctcaatc tgccccgcac atggccattg atgtccaagc
    11521 actagactgt gatttctttg ctttcagcgg acacaaaatg tgcggaccaa caggtattgg
    11581 tgttttatat ggtaaacgaa aatggttaga agaaatggaa cctgttgaat ttggtggcga
    11641 aatgatcgat tttgtcgaat tatacgacag tacatggaaa gaactacctt ggaaattcga
    11701 agcagggaca ccaaatatcg ccggcgctat cgcattaggc catgcaatcg attatttgca
    11761 aaaactcggt atggacaata tccatcgata tgaagaagaa ctagccgctt atgtgttgcc
    11821 aaaattacaa gcaattgatg gattgactgt ttatggtcca caagacccga attttcggac
    11881 aggagtcgtt gcattcaatt tggatgggtt acatccccat gatgtcgcga cagctttaga
    11941 catggaaggg gtagccgtca gagcaggtca tcattgtgcg caaccgctat tgaagtactt
    12001 tgaagtatca gcgactgctc gcgcgagctt ttatttctac aacacaacgc aagacgccga
    12061 ccgtttagtt gaagcaatca tggcaacaaa ggagtttttc caacatggga ctatctaaat
    12121 tagacaattt ataccgtcaa gtgatcttgg atcattcttc ccatccgcat catcgtggaa
    12181 gcttgactga ctcgacaagc aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg
    12241 aattgcaagt cgatgtaaaa gacggaatca tcaaaaatat cgcctttgac ggcagtggct
    12301 gctccatcag tacggcaagt gcctcgatga tgacagacgc cgtcatcgga aaatcaatag
    12361 aagaagcaga acatttaact gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag
    12421 atcccgacca attaggcgat gctgctatgc ttggtggtgt agctaaattt cccgctcgga
    12481 tcaaatgtgc gacacttgcc tggaaagcac taggaaaagc aatggaaaac gatggtaccg
    12541 cagctttcca tcattaccac aaggaagagg agtgagaaca tgggcgtacc agaattagaa
    12601 gaatataaat tcgggtttca cgatgatgtc gaaccggttt tcagtacagg agaaggcttg
    12661 acagaagaag tgatccgtga gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa
    12721 ttccgtctga aatctctaga aacatttaac aaaatgccaa tgcaaacatg gggccccgac
    12781 ctttcagaca tcgatttcga tgccatcaaa tattaccaaa aaccaagtga caaacccgct
    12841 cgtgactggg aagacgtacc agaaaaaatc aaagatacat ttgaaaagat tggtatacca
    12901 gaagctgaac gagcttatct agccggtgct tccgcacaat atgaatcaga agtcgtgtac
    12961 cataatatga aagatgaatt cgaaaagtta ggaatcgtct ttactgacac tgactctgct
    13021 ttgaaagaat accctgaatt attcaaagag tacttttcaa aacttgtacc gccaaccgac
    13081 aacaaattag cagcgctgaa ttcagccgta tggtcaggtg ggacctttat ttacgagcca
    13141 aaaggcgtca aagtcgatgt tccattacaa acgtacttcc ggatcaatgc ggaaaacacg
    13201 gggcaattcg aacgtacgct gatcattgtt gacgaaggag caagcatcca ctatgtcgaa
    13261 ggttgtacgg cacctactta ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt
    13321 acaagaaaag atgcgtattg ccgttacaca acgatccaaa actggtcaga caatgtgtac
    13381 aacttagtaa ccaaacgagc aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga
    13441 aacctaggtg caaaaacaac gatgaaatac ccaagtgttt acttagatgg aaaaggtgca
    13501 cgaggcacca tgctctccat cgcctttgca ggagcaaacc aaatccaaga tacgggtgca
    13561 aaaatgatcc acaatgcacc aaatacatct agctctatcg tctctaaatc aatttcaaaa
    13621 gacggcggag aagtaaacta tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg
    13681 atctctcata tcgaatgtga cacgatcatc atggacgaat ggtcgaaatc cgatacgatc
    13741 ccattcaatg agatccacaa cagccaagtc tctttagaac acgaagccaa agtctcaaaa
    13801 atctcagaag aacaactcta ctatctgatg agccgaggcc tgaccgaatc tgaagcaact
    13861 gaaatgatcg tcatgggctt tgttgaacca ttcaccaaag aacttccaat ggaatatgca
    13921 gtcgaactca atcgattgat cagttatgag atggaagggt ctgtaggtta ataactattt
    13981 cattgctcga aacgttgata ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg
    14041 ttggctacgt ttttggctac gtttggaatt attcgcccat aaattttgca aatcggtcac
    14101 cagttttctt aacagattgt ggtgtgacat gagcatagat attcatggta gttttaatat
    14161 ctttatgacc taatcgttcc tgtacttctt ttatcgacgc accactttca aacaataagc
    14221 tacagtgggt atgcctaaat ccatgtggag taatttgagg ctccatcggt gtctttttgt
    14281 aaatccagtc taaccaatcg tttactactt gggggtagta caacttgttt gtgtttgtag
    14341 tgaaaagaaa ctgttttggt gaattcgtgt tatagccata taagatcatt attttcattt
    14401 gttcttgttg ccaaatacgc agttgtttaa gtgtaaaatc atcaagctga atcgttcttg
    14461 atgaattttt agttttaggt acttgtagaa ctacttgatt aaactcgtct tgagcgatag
    14521 ttttaccaat tgttattgtt ttattgaaat agtctatatc ttcccattga agcgccaatg
    14581 cttcgctttt acgcattcct gtataagcta ataaacggaa aaaagtgtac agtttaactg
    14641 aattattttc tttagtattc tgtaaaaaca gttgtaattg atctttcgtg taaaacttca
    14701 agggttgttc ttcttcaatt tttcttggaa gcaatgtttt agacatagga ttatccttac
    14761 agacttccat tgctactcca aatttaaata tttgagcagt tgtttttctc aagaaaccat
    14821 attgttttaa tgttttgtgc cattcattca cctgtttttg gcaatagcta acacttattt
    14881 tatccaatcg taattcccca aaagcagata atatatggtt tttgcaaaaa cgttgagcaa
    14941 cggcaacaga agacggtttt actcttgttc tgtagttatc aatccataat tcatacaact
    15001 cttgaaaagt tgtaacattg attttaaact cttgtttttc tttaaagtct acaagtagtc
    15061 tattaagtgc tgtttgcgcc tcagcttttg atttgaatct tctacgcttt gtacgtatat
    15121 cttttccagt cttgggatct ttccctaaat aagtttcaaa tccccaatat ttttcgccat
    15181 tttgtaattt ataactaaaa aatgttgcca tttgattctc tccttacttc attattgc
//
LOCUS       DAYYGS010000062        14965 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000062 DAYYGS010000000
VERSION     DAYYGS010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14965
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.062"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(148..714)
                     /locus_tag="ACHA44_001955"
     CDS             complement(148..714)
                     /locus_tag="ACHA44_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8905222.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(714..1670)
                     /locus_tag="ACHA44_001956"
     CDS             complement(714..1670)
                     /locus_tag="ACHA44_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFM8905223.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1670..2326)
                     /locus_tag="ACHA44_001957"
     CDS             complement(1670..2326)
                     /locus_tag="ACHA44_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HFM8905224.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2696..4714
                     /locus_tag="ACHA44_001958"
     CDS             2696..4714
                     /locus_tag="ACHA44_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8905225.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    4784..4990
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5141..7555
                     /gene="leuS"
                     /locus_tag="ACHA44_001959"
     CDS             5141..7555
                     /gene="leuS"
                     /locus_tag="ACHA44_001959"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFM8905226.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            7903..9456
                     /locus_tag="ACHA44_001960"
     CDS             7903..9456
                     /locus_tag="ACHA44_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HFM8905227.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(9837..11621)
                     /locus_tag="ACHA44_001961"
     CDS             complement(9837..11621)
                     /locus_tag="ACHA44_001961"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905228.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11636..13357)
                     /locus_tag="ACHA44_001962"
     CDS             complement(11636..13357)
                     /locus_tag="ACHA44_001962"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905229.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13457..14218
                     /locus_tag="ACHA44_001963"
     CDS             13457..14218
                     /locus_tag="ACHA44_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8905230.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4866 a         2731 c         2958 g         4410 t
ORIGIN      
        1 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
       61 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      121 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      181 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      241 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      301 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      361 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      421 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
      481 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
      541 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
      601 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
      661 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
      721 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
      781 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
      841 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
      901 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
      961 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1021 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1081 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1141 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1201 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1261 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1321 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1381 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1441 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     1501 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     1561 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     1621 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     1681 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     1741 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
     1801 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     1861 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     1921 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     1981 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2041 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2101 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2161 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2221 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2281 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2341 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2401 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     2461 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     2521 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     2581 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     2641 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     2701 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     2761 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     2821 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     2881 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     2941 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3001 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3061 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3121 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3181 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3241 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3301 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3361 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3421 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     3481 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     3541 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     3601 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
     3661 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     3721 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     3781 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     3841 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     3901 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     3961 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4021 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4081 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4141 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4201 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4261 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4321 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4381 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4441 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     4501 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     4561 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     4621 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     4681 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     4741 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     4801 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     4861 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     4921 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     4981 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5041 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5101 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5161 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5221 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5281 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5341 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5401 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     5461 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     5521 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     5581 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     5641 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     5701 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     5761 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     5821 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     5881 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     5941 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6001 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6061 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6121 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6181 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6241 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6301 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6361 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6421 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     6481 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     6541 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     6601 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     6661 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     6721 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     6781 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     6841 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     6901 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     6961 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7021 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7081 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7141 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7201 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7261 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7321 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7381 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7441 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     7501 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     7561 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     7621 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     7681 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     7741 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     7801 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     7861 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     7921 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     7981 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8041 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8101 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8161 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8221 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8281 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8341 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8401 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     8461 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     8521 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     8581 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     8641 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     8701 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     8761 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     8821 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     8881 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     8941 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9001 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9061 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9121 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9181 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9241 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9301 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9361 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9421 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
     9481 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
     9541 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
     9601 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
     9661 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
     9721 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
     9781 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
     9841 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
     9901 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
     9961 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10021 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10081 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10141 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10201 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10261 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10321 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10381 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10441 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    10501 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    10561 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    10621 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    10681 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    10741 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    10801 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    10861 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    10921 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    10981 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11041 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11101 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11161 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11221 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11281 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11341 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11401 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    11461 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    11521 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    11581 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    11641 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    11701 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    11761 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    11821 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    11881 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    11941 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12001 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12061 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12121 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12181 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12241 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12301 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12361 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12421 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    12481 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    12541 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    12601 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    12661 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    12721 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    12781 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    12841 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    12901 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    12961 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13021 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13081 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13141 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13201 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13261 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13321 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13381 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13441 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    13501 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    13561 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    13621 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    13681 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    13741 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    13801 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    13861 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    13921 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    13981 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14041 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14101 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14161 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14221 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14281 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14341 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14401 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    14461 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    14521 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    14581 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    14641 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    14701 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaag
    14761 aaaaatcaca aaggttattg ttatttttat atattctttt taagaatgat tgttttttta
    14821 tagaaatttt aaaaaataaa acacgttatt gatggatagt tagtttgtta tttggtaata
    14881 ttatagaagt tgactggtag tgatttattt ttcgtcgttt tataaaaatg ttattttttg
    14941 gaaaaattca caaatatgta acgaa
//
LOCUS       DAYYGS010000063        14960 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000063 DAYYGS010000000
VERSION     DAYYGS010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.063"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            4..2376
                     /locus_tag="ACHA44_001964"
     CDS             4..2376
                     /locus_tag="ACHA44_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFM8905231.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="ACHA44_001965"
     CDS             2437..3897
                     /locus_tag="ACHA44_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFM8905232.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="ACHA44_001966"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="ACHA44_001966"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8905233.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKINQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="ACHA44_001967"
     CDS             6128..6277
                     /locus_tag="ACHA44_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905234.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="ACHA44_001968"
     CDS             6352..6945
                     /locus_tag="ACHA44_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HFM8905235.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="ACHA44_001969"
     CDS             6958..7857
                     /locus_tag="ACHA44_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HFM8905236.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="ACHA44_001970"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="ACHA44_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFM8905237.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="ACHA44_001971"
     CDS             8723..8983
                     /locus_tag="ACHA44_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905238.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="ACHA44_001972"
     CDS             9428..9634
                     /locus_tag="ACHA44_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HFM8905239.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9655..9885
                     /locus_tag="ACHA44_001973"
     CDS             9655..9885
                     /locus_tag="ACHA44_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905240.1"
                     /translation="MTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLVEDAPTML
                     FEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="ACHA44_001974"
     CDS             9901..10338
                     /locus_tag="ACHA44_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905241.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="ACHA44_001975"
     CDS             10331..11038
                     /locus_tag="ACHA44_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905242.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11202..11402
                     /locus_tag="ACHA44_001976"
     CDS             11202..11402
                     /locus_tag="ACHA44_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905243.1"
                     /translation="MNKIIPLLFVLCIGEILMRHGAAVHLNFVVFLGILVFCLIFWRF
                     VLALVSLLFKLIAAIFFIGLFR"
     gene            11418..11597
                     /locus_tag="ACHA44_001977"
     CDS             11418..11597
                     /locus_tag="ACHA44_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905244.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="ACHA44_001978"
     CDS             11922..12185
                     /locus_tag="ACHA44_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HFM8905245.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="ACHA44_001979"
     CDS             12179..12529
                     /locus_tag="ACHA44_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8905246.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(12553..13167)
                     /locus_tag="ACHA44_001980"
     CDS             complement(12553..13167)
                     /locus_tag="ACHA44_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905247.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            13481..13903
                     /locus_tag="ACHA44_001981"
     CDS             13481..13903
                     /locus_tag="ACHA44_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905248.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            13913..14116
                     /locus_tag="ACHA44_001982"
     CDS             13913..14116
                     /locus_tag="ACHA44_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905249.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            14327..14911
                     /locus_tag="ACHA44_001983"
     CDS             14327..14911
                     /locus_tag="ACHA44_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905250.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAINFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         5514 a         2429 c         3032 g         3985 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaagaatac
      241 gtggaaaagc ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgg ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctaga
      661 ccaactgatt ctatgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt cgaattgatc
      781 gatcaactca aaacattgga taaccaatac gccgcagaag caggttatta taaattctat
      841 tttgaggaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaaccaat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaataaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggtcacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt ttgcaatctt agcgatcgca acaccttttt ttagatcctc
    12661 aacgatgttt ttataaacaa gacgacgttg gggatctttt gcattggcac tgtatagctt
    12721 aggacgtcct ttataaacac cgttcgcttt tgcaatctta atgccttgtg cctgacgtcg
    12781 tttagattcg gtacgttctt gttcagcgat catggccaaa atttgaatga tcaaatcttt
    12841 aatgaattta tccaggagag gattgccaat tgcttcggcc atgatcggta gacttgtgat
    12901 gatcagctgc acatttttct tcttcaagta attgactgaa gcgatgatct cgtcgtaatt
    12961 tcttccaagt cgatcaatag cttccacaat aaaaatatct tgatctcgga cgaaatccag
    13021 tgcttcttga aaaacaggtc gatgagtaat cgttgcgcca gattgtttct caatgaaaat
    13081 tctctctgca ccgaattttt tcatttcttc aatctgccgt tgttcatttt gatcgatgga
    13141 tgagacacgt atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaaa
    13201 acaccctaat catacacgag aaaaccagat ttttcaatgt cgcggtcgtg tctagttagg
    13261 gtatacctta tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt
    13321 taaatggcaa tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat
    13381 taaaaatata ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta
    13441 agaaaacgtt ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt
    13501 atctttaggt attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc
    13561 tgatagtaca aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga
    13621 taggaacgga atagccattg gtaacccaac taacgactta actgttactg aaaattattt
    13681 aatagtttct ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat
    13741 gatttttgat aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa
    13801 aagaaatggt tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa
    13861 caattggatt gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga
    13921 atttgtgaac acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc
    13981 gaaagaagaa tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa
    14041 atcaatttct aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata
    14101 tcaaaaagaa aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa
    14161 ttgaaagtgt tcataaaatc acccatttct ggacactttc tttttttgct ttatttttca
    14221 aaaaaacaat tcatttcatg ttcataaacc cataacaata tctatgttca gttaaactat
    14281 atggataaag ttatgttaca ataaaattaa tgaaggagtg atttttttga aaataggata
    14341 cgcccgggta tctactggtt tacaaaattt ggatttacaa gaagacagcc taaataaatt
    14401 tggttgtgaa aaaattttta ctgatcatat gagtggggca aaaagtaatc gaccaggatt
    14461 agaaatggcc attaattttg ttcgctctgg cgatacactt gtcgtttggc gcttagatcg
    14521 tttaggtaga aatatggaag atttgatttc aatcgtcaat cgactgaatg agcgtggtgt
    14581 gagttttcat agtattcaag aaaatattac gatggacaaa tcaagttcta caggacagct
    14641 catgtttcat ttgtttgcgg catttgcaga atttgagcgt aatttgatcc tggaacgatc
    14701 cgcagcagga cgagcagctg ctcgtgcaag aggacggttt ggtggtcgtc ctgaaaaatt
    14761 gtctgaacaa gatttggaat tactgaagac tttagtggat agcggtacac ctattaagac
    14821 tattgcggaa cggtggaacg tctcaagaac caccatttat cgttatttgg caaaaatgga
    14881 ggagaaaaat ggaaagacaa attctcatta gtttttgtca tttaatgctg aaaatttttg
    14941 ccatttatct agaaaagtga
//
LOCUS       DAYYGS010000064        14479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000064 DAYYGS010000000
VERSION     DAYYGS010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.064"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(227..1114)
                     /locus_tag="ACHA44_001984"
     CDS             complement(227..1114)
                     /locus_tag="ACHA44_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905251.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="ACHA44_001985"
     CDS             complement(1111..1980)
                     /locus_tag="ACHA44_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905252.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="ACHA44_001986"
     CDS             complement(2117..3403)
                     /locus_tag="ACHA44_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905253.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="ACHA44_001987"
     CDS             3676..4254
                     /locus_tag="ACHA44_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFM8905254.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="ACHA44_001988"
     CDS             4254..5183
                     /locus_tag="ACHA44_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905255.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="ACHA44_001989"
     CDS             5180..6916
                     /locus_tag="ACHA44_001989"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFM8905256.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA44_001990"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="ACHA44_001990"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFM8905257.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="ACHA44_001991"
     CDS             complement(7812..8321)
                     /locus_tag="ACHA44_001991"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFM8905258.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA44_001992"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="ACHA44_001992"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFM8905259.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9622..10713)
                     /locus_tag="ACHA44_001993"
     CDS             complement(9622..10713)
                     /locus_tag="ACHA44_001993"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFM8905260.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA44_001994"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="ACHA44_001994"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFM8905261.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA44_001995"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="ACHA44_001995"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFM8905262.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA44_001996"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="ACHA44_001996"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HFM8905263.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         4130 a         3149 c         2616 g         4584 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagcttctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgt
//
LOCUS       DAYYGS010000065        14345 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000065 DAYYGS010000000
VERSION     DAYYGS010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.065"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(93..1391)
                     /gene="eno"
                     /locus_tag="ACHA44_001997"
     CDS             complement(93..1391)
                     /gene="eno"
                     /locus_tag="ACHA44_001997"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFM8905264.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(1507..2262)
                     /gene="tpiA"
                     /locus_tag="ACHA44_001998"
     CDS             complement(1507..2262)
                     /gene="tpiA"
                     /locus_tag="ACHA44_001998"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFM8905265.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(2366..3556)
                     /gene="pgk"
                     /locus_tag="ACHA44_001999"
     CDS             complement(2366..3556)
                     /gene="pgk"
                     /locus_tag="ACHA44_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFM8905266.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(3666..4667)
                     /gene="gap"
                     /locus_tag="ACHA44_002000"
     CDS             complement(3666..4667)
                     /gene="gap"
                     /locus_tag="ACHA44_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFM8905267.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(4708..5799)
                     /locus_tag="ACHA44_002001"
     CDS             complement(4708..5799)
                     /locus_tag="ACHA44_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HFM8905268.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(5948..7282)
                     /gene="rpoN"
                     /locus_tag="ACHA44_002002"
     CDS             complement(5948..7282)
                     /gene="rpoN"
                     /locus_tag="ACHA44_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFM8905269.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            7342..7413
                     /locus_tag="ACHA44_002003"
     tRNA            7342..7413
                     /locus_tag="ACHA44_002003"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7375..7377,aa:Arg,seq:ccg)
     gene            complement(7605..8489)
                     /locus_tag="ACHA44_002004"
     CDS             complement(7605..8489)
                     /locus_tag="ACHA44_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFM8905270.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(8576..9475)
                     /locus_tag="ACHA44_002005"
     CDS             complement(8576..9475)
                     /locus_tag="ACHA44_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HFM8905271.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(9492..9998)
                     /locus_tag="ACHA44_002006"
     CDS             complement(9492..9998)
                     /locus_tag="ACHA44_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8905272.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(10187..10675)
                     /gene="rpoE"
                     /locus_tag="ACHA44_002007"
     CDS             complement(10187..10675)
                     /gene="rpoE"
                     /locus_tag="ACHA44_002007"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HFM8905273.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDYEEEEFSDSE
                     KE"
     gene            complement(10751..11188)
                     /locus_tag="ACHA44_002008"
     CDS             complement(10751..11188)
                     /locus_tag="ACHA44_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HFM8905274.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            11351..12181
                     /locus_tag="ACHA44_002009"
     CDS             11351..12181
                     /locus_tag="ACHA44_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HFM8905275.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            12165..13532
                     /locus_tag="ACHA44_002010"
     CDS             12165..13532
                     /locus_tag="ACHA44_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8905276.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            13551..>14345
                     /gene="yidA"
                     /locus_tag="ACHA44_002011"
     CDS             13551..>14345
                     /gene="yidA"
                     /locus_tag="ACHA44_002011"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFM8905277.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIK"
BASE COUNT         3900 a         2985 c         2656 g         4804 t
ORIGIN      
        1 tatttcagca agtagagagt tcgattactt aactaagtga tcatactcga ctatctttca
       61 atagtcgatc atgtatcttc attagaaaag aattatttgt tttttaagtt gtagaaagat
      121 ttcaaacctt tgtattctgc aacttcacca agttggtctt cgatacgtag taattggttg
      181 tatttagcaa tacggtcagt acgtgataga gaaccagttt tgatttggcc agcgtttgtt
      241 gcaacagcga tatcagagat tgttgaatct tctgtttcac cagaacggtg agatacaact
      301 gctgtgtagc cagcttcttt agccatttcg atagcttcga aagtttctgt taatgtaccg
      361 atttggttca ctttgataag gattgagtta gcaattcctt tttcgatacc ttcagataat
      421 ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt gaactttgtc gcctaatacg
      481 tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta atccatcttc gattgagatg
      541 attggatatt tagaaactaa ttcttcgtag aatttgatca tttcgtcagt tgttttttcg
      601 ccttcgcctg agtcagctag tacgtaaaca cctttttctt tgtcgtagaa ttctgaagaa
      661 gcagcatcca tagcaagaac aacgtcttta ccaggtacat agccagcttt ttcgattgct
      721 tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt ttggagcaaa tccaccttcg
      781 tcaccaacag aagttgctaa tccgcgagct ttcaagattg aagctaatgc gtggaatact
      841 tcagcaccca tacgtagagc ttctttgaat gttggagcgc caacaggcat gatcatgaat
      901 tcttggaagt cgattgagtt atcagcatgt gatccgccgt tgatgatgtt catcattggt
      961 gttggcaata cttttgtgtt gaatccgcct aggtagtggt ataaaggtac ttctaggtag
     1021 tcagcagctg cacgtgcaac agcgatagaa acacctaaaa tagcgttcgc acctaattta
     1081 cctttgttag gagttccgtc taaagcgatc atagctttgt cgatagccat ttggtcacga
     1141 acatcgtagc caatgattgc ttcagcaatg atgttgttta cgttgtcgac agctttagtt
     1201 acacctttac cgccataacg agctttgtct ccgtcacgta attcaactgc ttcgtattca
     1261 ccagttgaag cacctgaagg aaccattccg cggccaaaag ctccgctttc tgtgtatact
     1321 tctacttcga ttgttgggtt accacgtgag tctaagatct cgcgagcgta aacatcagta
     1381 ataattgaca tgttttgtct ctcctttgag tttgtttttt cctaagggat ttccctcata
     1441 tacgatttta gttaattttg ctcattagtg caaacatttc ttgcattttt cgcaaatttt
     1501 caacaattat tttacagcgt ctaacaatga caagaatgaa tctgcttgca agcttgcgcc
     1561 accgactaaa gcaccgtcaa cattttcttt tgccatgtat tcagcaatat tttctggttt
     1621 tacagaaccg ccgtattgga tacgaactgc ttctgatact tcttttccat aaagtttttc
     1681 aactgtttga cgtacaactc cacaaatttc gtcagcgatt tgtgcgtcag ctgatttacc
     1741 agtaccgatt gcccagattg gttcataagc aataaccatg tttgacacct gttcagctga
     1801 aagatcagct aaaccagctg taatttgtcc ttcgatccat tcagcagttt tgcctgcttc
     1861 ataggtttct aaagattcgc cgcagcatag gattggtgtc ataccatttg caaagatagc
     1921 tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa tattcacgac gttctgaatg
     1981 tccgatgatc acataatgaa cacctagatc agcaagagct gctggtgaag tttcaccagt
     2041 gaacgcacct gagttttccc agtagcagtt ttgtgctgag atttttagtt ctgtaccttc
     2101 agctgatgtc attaattctt gtaagaataa tgcaggagat ccgatgactg aatcaacttt
     2161 gtcatttgaa gggattttgt tttttactgc ttcagcaaat tcttttgctt ctgatgcagt
     2221 tttgttcatt ttccagttac ctgcgataat aggtttacgc attgaggaac acactcctta
     2281 atttattgaa aaatggctag ggagtcatga aaactccctt caccttttgt tcgattactc
     2341 gtcagaattg agcaaacttg gtcgtttatt tatcgttgat tgctgctagt cctggtaatt
     2401 ctttaccttc aagcaattcc aagcttgcgc cgccacctgt tgagatgtgt gtgaatttgt
     2461 cagcaaatcc taattgttct gctgctgcgg ctgagtctcc gccaccgatg attgttgttg
     2521 cgccttctaa gttagcgatt gcttcacaaa caccgattgt acctttagca aagttactca
     2581 tttcaaatac acccataggt ccgttccaaa caactgtttt tgcaccttgt aatgtatcag
     2641 caaataattt tactgatgct gggccgatgt ctaaagccat caaaccatca ggaatgtctc
     2701 cttcgattac ttgtgtttca acatcatttg aaaattctgg tgcacagaca ttgtcgattg
     2761 gcaagacaag tttgtcgcct gctttttcga ttaattcttt tgctagttcc actttatcag
     2821 cttctactag agagttaccg atcttgattc cttttgcttc gtagaatgtg taagtcattc
     2881 cgccaccgat caagatttta tctgctttag gaatcaagtt ttcgattacg ccgattttat
     2941 cagaaacttt tgcgccacca aggattgcaa tcattggtcg ttttggttct tctactgctt
     3001 caccgatgaa cttgatttct ttttccatca agaatccagc aactgttggg ataccagttg
     3061 aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt accgaaagca tcgttaacaa
     3121 atacatcgcc taatgaagcc cagtatttac ctagttctgt atcatttttg ctttcttttt
     3181 taccgtcaat atcttcaaat cgtgtatttt cgaatacgac aacgtcgccg tcattcatgt
     3241 tgcggattgc ttcttctaat tgttctccgc gagtttcagg tacgaatgtt acttctttac
     3301 ctaataattc gcctagacgt tttgcaactg gagctaaaga ttttccttct ttatcttctt
     3361 ctgtttttac acgtccaagg tgagagaaaa ggattgcttt tccgccttgt tctagaacgt
     3421 agttgattgt tggcaatgct gctttgatac gagtgtcgtc agtgatcacg ccgtctttca
     3481 aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc ttttaaatcg atatctttca
     3541 ctgttttctt agccatttta tttcctcctg aataaactta ttatcaaaaa aagcggaaga
     3601 agcgcgattg cttccccgct tttaaatgta catgtctata atttacactt gtacgtttgt
     3661 ctatcttata agttagcgaa gtattctaaa gtacgtacta attgtgcagt gtatgacatt
     3721 tcgttgtcgt accaagcaac agttttaact aattgtttgt cgccaactgt cattacttta
     3781 gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa cgatatctga agaaacgatt
     3841 tcgtctgtgt tgtatccgta agattcgttt gaagcttctt tcattgctgc attgatttcg
     3901 tcaacagtta cttctttttc aagaactgtt actaattctg ttaatgaacc tgttggtaca
     3961 ggaacacgtt gagcagctcc gtctaattta ccgttcaatt cgggaattac taaaccgata
     4021 gctttagcag cacctgttga gttaggaacg atgtttgcag cagcagcgcg tgcacggcgg
     4081 aagtcacctt taggatgagg tccgtctaga gtcatttggt cacctgtgta agcgtggata
     4141 gttgtcatta atccttcaac aacaccaaat ttgtcgttca atgttttagc cataggagct
     4201 aaacagttag tagtacatga agcacctgag ataactgttt ctttaccagt taatgtttcg
     4261 tggtttgtgt tataaacgat tgttggaaca tcgtttccgc caggagcaga gataacaacg
     4321 cgtttagcac cagctgttaa atgtttttca gctgctgatt tagaagtgaa gaaaccagta
     4381 cattctagaa cgatatctac gcctagttcg ccccatggta attcttctgg gttgcggtta
     4441 gctaatactt taacttcttt tccgtttacg ttgaatgaac cttcatgaac ttcaactgtt
     4501 ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt gtgccaacat tttagcatct
     4561 gtcaagtcgt tgattgctac tacttcgatt ccatctacat cttggatacg acggaatgct
     4621 agacgtccaa tacgtccaaa accattaata cctactttaa ctgtcattta agatttcctc
     4681 cttatgaaaa tcaaaaaaat atttatttta aagggttacc ccttttaaaa tctcatttgc
     4741 agcagcttca tcagtgatga gccacgtttg ttttggtgca tttttcatat aagctcgaat
     4801 tgctttagct tttgattttc cgcccgcgat tgccatgatg atcgggactt tttccaaatc
     4861 ttgcaattct atgccgattc tagggacctt ataaacgact tcaccttttt cattgaaaaa
     4921 gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc atgactaatt ctttttcagt
     4981 catcttacga cgtgctgcca tgtgtaatgc acgcccaata ctatgaataa cacaattcgc
     5041 ttcactaatc aagtttaaga cttcttggat agatggctca ttgagtaaag aattgtaagt
     5101 tgccagactt aattgttccg gaacataaag tgcgcggtgg ttaccgtttg ctttagcagc
     5161 cattcttgca ctgacagaat ttgcctgtac agttatcgct tcaccaatcc cgcctcttgc
     5221 agggacaaac aggttatgcc gtttcttatt ttccagatta gacaattgtt cggctaccac
     5281 agccatagta gttcctccca tcacagcgat gatattctcg ccttctggaa gtagtcggtc
     5341 taaggcttcg ctcagcgctt cgccgaatgc atcagccact ttggtctgct cttcgctgtt
     5401 tcctgaaaca acgatacagc gttggttacc aaaatattct gccaactgtt gttcggtttg
     5461 atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt tgataaactt cttcgccctt
     5521 agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa ttcagatttt tcaaaaggtc
     5581 tgtttctgtt cgtaagactc gttctgtgat ccccatactt tccgataaac ttcgacgccc
     5641 aatcggctgc atccaataga tatttcgtaa aattttatag cgttcttgca acacttcgat
     5701 aatatctggt gcaacagatt caatcatttt tatttcatcc agcatagcga tctccttagt
     5761 tggacacttt acgtccaatg tagacttaaa acgacccata tacgataaaa aaagaaatta
     5821 actattcgaa tttcacatca ataaagggaa atctcaacct cacgactgta agtctaacag
     5881 tttcgctctc gtttttcaac taaaaagact agaaaaagtg aaatactttt tctagtcttt
     5941 tgactgatta gttatcataa cgttttcttt ttgatgaact cgggattcct aataaatcac
     6001 gatattttgc tactgttcgt cttgagatcg ctatctcatc ttttttcagc aattcaacca
     6061 atttctggtc cgacaacggc ttgtttttgt cttcttgatc gactaattcc tgcagctttt
     6121 tcttcgctgt atctgctgaa aggtcttctg tctgttctgt actattcgta ggaattcgtg
     6181 tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt ttccaaatat ttcccgttga
     6241 ctgcacgact gaccgtggat tcatgcacat ctatctcagt tgcaacttcc tttaacgtaa
     6301 gaggcttgat tggacgttcc ttgtcgataa agaaagcttt ttgccgggaa acaatgactt
     6361 gagcaacccg caaaattgta tccccacgtt gtaggatcgt tttcttcaac cattcgaatt
     6421 cctgtttccg ttcttttaaa taattttcag tttctttatc tgcttgttgt gacatttgtt
     6481 tgaagtaact ctcttgaaag cgtatatttg gctgattttt tcggtttgag ataacttgta
     6541 catttttatc gtcgtccact aatacccgga catcaggaat aatgtacaat tcgctgctac
     6601 ggtcgaaaat ccggccaggg cttggactca acgtttgtaa atagtcgaat atctgctgaa
     6661 cagcatgtag atcaacagaa aaacgttgtg cgatttcttt ccattttcgc tccaccagtt
     6721 cctcaaaaca ttcttccaac acgatataag ctaattccgg cgcataatca tctcgttctg
     6781 tttgaagcat gagacattct tgcaaactac gtgcacctac tccagctggt tctaattgct
     6841 gaatcagagt caacgcatcg agcatttgga taggcgtagc tccaatctct tttccggctt
     6901 cttctaaact aatcgtcaaa aatccgttca aatcgatgta ttctacaaga taaagaacga
     6961 ttttcctcaa aaaagtatct cggtaattca aatggacttg ttcgatcaat gaatcaaata
     7021 aagacccttt tgtatcagga atctgattta ggtaatttgt ctcttctcct tcataagaag
     7081 atgaagaagc ttttatgtaa tctggctgcc attccggctc aacaacttcc accaatggat
     7141 tttccattgc ttgattctca acgaatgcca acagctcctc actgttatat tgtaaaactt
     7201 ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg tgtctgctgc tgtttttgtg
     7261 aaaaattttg ctcaaatttc atttttttgt ccaatcccct tgtaattttt tatctgttta
     7321 gttacaatta tcccagtgct tgcgtcctta gtgtagtgga tatcacgtaa gattccggtt
     7381 cttgagacgg gggttcgatt ccctcaggac gcgttctact gctattttag cataatttac
     7441 gaatagttgc ttctttattt ttaagtgaaa actgtccgcc aaagaaaatt ggtggacagt
     7501 tttttgttgt acctgtatag atattgttaa gcctttgcaa gtaaggtagt tatgtgttca
     7561 actacttatt aaaatgtgtt atttattaaa gatacttgtc tcacttactg aactggccga
     7621 tgatcgtttc tgccataata gttctcgaca gccgttggat gccctaataa atccccaact
     7681 gtcgtgatgc gatacccttg ctctttcaga taatcgatca cttgaggaac ggcacgaatc
     7741 gtttcaggat agatatcatg gaataaaata attgccccat cgtatactgt tgcttggatc
     7801 ttctgcaaaa tcaaatcagg atttcttgtc ttccaatctt ctgaatcaac agaccattcg
     7861 atgatcggtc gctggatgat cgttgctcct acattcgtga cagaaccata gggcggacga
     7921 acaaaagtgg gggttttgcc agtgactttt ttgatctcag tgtctgcttt cgtcacttcc
     7981 tccgtcatcg actgggcaga aagagtggcc agattctgat gatcgtatgt gtaattgcca
     8041 atctcgtgcc ctgcttcagc tgtctgcttc acgatttcag gatgctgcgc cgtattttct
     8101 cctaagacga aaaaagtggc atgcacgttt ttttctttta agattcgcag aagctgcggt
     8161 gttgtggtcg ctcctggtcc atcatcaaag ctgattgcga cgcttttgac agccggatct
     8221 cggctcgttt ttgtactgtc ttgtgttgat tgtgatgact gactttgtac cttttccgct
     8281 gattcttgaa tggcttcttt gacgttagct gtttcagttg attggctttt aatggttgat
     8341 tgttctgatt gtttgaaaga ttgtgacgaa tgctccgaag agtgtgtttg tccttttact
     8401 tcggtgatca aggaggccac cgaagtaatt tcgaaaaaca aaagcacgga taaagcaata
     8461 atactgataa ctaaataatt tcttttcact atatctcttc tttctctttt tctcttctta
     8521 ttatttactt gtacttactt gaaaattgaa actgctagtc gcaactactt ctcgtctaat
     8581 gatccaaata atcttccgat gccaaaatcg tttttgtcac ggacttagga atgacaaatt
     8641 ctggtgtacc catataacca ggagcaattt cgtattgaga aaaagtcaaa actaattgat
     8701 gctctttgtt caaataaaaa cttcgtgtag gcgtaacaag tgttggtttt tctatcgtcc
     8761 cttcttgaat atcttgctca gtccaatagt acttagatgg atcttccttg acttgttcgg
     8821 ccatctgccg tttgacttct tttgtcaaaa cagatagata ggtgtcatct ttgaataaaa
     8881 gtggaagtga gagctggaca ctgtttttct tgtcgattgt gtcgtatctt ttttcaacat
     8941 ggctatctgc tgctgtttgg gtgatctgcc tttcaatcac gagaaaccgg ttatcgtcta
     9001 cgattttctg ataatctccc aaaacttggt aatgatccgt ttcaaaattt ccatattctt
     9061 tttcagcttt ttcaaaatct gcttgtcctt cgcgaaagta tttttgattt aatccatgaa
     9121 ttgttttatt ttctttctca ttttgtgtat tgatcttcgg tacctgaata tcgatttttt
     9181 cagatcgatc agtaaagcgt tcgcctaaga tcaattcaat gactgatcct aagatgggta
     9241 aatcttctgc aaagcttcga acttgactat taaaaagaag gctacttgtt gtaatcatta
     9301 cgatagccaa agatactccg aacccttgga gacgtctctt tctccgcttt gcttttataa
     9361 atgattgctc ttcgataata aaacgctggc ggatctcctc tttaagccct ttgggaattg
     9421 cttgttcttt ataagttttc tttagttttt ttaaattttc tttttgggaa tacatggcac
     9481 tcttctctcc ttcattctag gatttgacgt aaagcattca atgagcggta cattttcgtt
     9541 ttaactgtgt tttcattcat atccaagata gtcgcaattt ctttcaaacg aaatccttca
     9601 aaaaagtaga ggatcagaat ctctttttcc ggactgtcta ttttatctaa aatcatatgg
     9661 agctctgcaa cggaagtttc taaaggtaac tgttggggga tatcttgaat ttcaaataag
     9721 tccacaggcg tccttttttg tctgcgccaa ctatctaatg ccgtgcggat caatatccga
     9781 taaaaccatg cactaaaatg ctcgatctct ttttcttttt caaaatagtt cagggcttta
     9841 tgaaaactat cttgcatcac atctaaggcc tcttctttat catgtacgta attcatcgtg
     9901 atacggaaca ttttttgata atcagtttcg atgagtccat tcagcaggtg acggtatttt
     9961 tcgatctgcc gttttttctt atatccagta aaaatcattc tccactcctt tctgcctcac
    10021 ttaatatgac gtacaaggtg cgctaaaagt ttcaattttt ttatttttta tgtatttact
    10081 tgggattttt atatacaaaa aagaaggtgt gacaaaaatc gtgtcacacc tccttattct
    10141 gataggctga tcgtctctgt cacaacctct cgtaagaaaa taatgtttat tctttttcag
    10201 agtcagaaaa ttcttcctct tcataatctt catcatcatc gtcatcaaac agatcatcgt
    10261 catcttcttc tgtcagatct gtatcttctg ggtcatcgtc attgtaatcg atcgcgtctt
    10321 catcatcatt gataaaggcg ttgactttct tgcgtttacg gcgacgtgga gtatcttcat
    10381 cctcttcgtc cattccatgg tttacttctt catcgatcga atcgatagca taccatgaac
    10441 gcaagcccca acgattatcc cctaatgaga taaagctgcc gtcaatattc aagtcagtat
    10501 aaaattgtgc gagtgaatca cgaatttcac tgtctgattt cccaagatag ttttggatct
    10561 ggttagctaa atcagaaaaa tccatgacat cgccacgttg ttctaagatc gcgtgtgcta
    10621 cttcgatcat tgataattcg cttttgtttg caccttcaaa tacactaatt tccaatcagg
    10681 acacgtcctt tcacagtcta ctccttatca tacaaaatca gacacaaaaa atcaaacttt
    10741 tcttttatat ttacgctgta aaatccaagg tcaaataata ttcgccgatt ttttccggtc
    10801 ccatgaacag atcataatcg attcgaacct tccctcctgt gggacggtct ttcaaactaa
    10861 catgcagttc tttcgtaaaa gtagcaatct ggatcatacc gtatggtgtt cgataggatg
    10921 tttctaaccg ttctttatag ccaaatttca aacgcatccg catttctcct gcacggatca
    10981 gctgtacatg accatctgga actaatttga tcgttacagg aatctcatcg cccgtctcct
    11041 cctgaatctc tttataacgt atgtacagcg tatcgccaat tgtcaccatt tgccctttca
    11101 aatcaaaata aaaatcttgc acttcccctt gctgttttac ttttgtacgc aatttgattg
    11161 aaatcggtgt tccttgtgat aattccatta ttcattctcc tttacttctc atagctacat
    11221 tgttcctagt ttcttttgtt ttgtcaacca acttgtccat ttttacagaa aacctttttt
    11281 ccaaaacaac tctttgattt tagagtataa tattgtagaa aatatgaatt tatacaaagg
    11341 agccggttga atgaaccctg cgattcgact agatcagcac atttatcaaa aaaacgactt
    11401 ttcccctttt gcattaacag atatattaac ggaatattct aaagaacacc agcaaatctt
    11461 tttacatttc tggcaatatg actgcacagt catcttaggt atgaaggatg ttcgaactcc
    11521 ttttctaagt gacgggatcc aaacactgaa agatgcaggt tacgctccag tgatcagaaa
    11581 ttcaggtgga ttagggatcg tatcagatga aggaattttg aatatttctt tgattttccc
    11641 ccaaagtgac acaaaaaaaa taaccataga tgacgcttac gagcaaatgc tcctattgac
    11701 taaatacgct tttccaaagg caccgataca agcattcgaa atcacccatt cttattgtcc
    11761 tggaacttat gatttaagca tccatggcaa aaaatttgcc ggtatcgctc agcgtcggat
    11821 caaaaatggg atttctgtta tgatgtactt aagtgtcaac ggtaatcagg aaaaacgcgg
    11881 gcaagtcatg aagcgatttt atcaatctgc tttaaaagaa gactttggta caaatggcta
    11941 tcctgcagtg gatcctgcta caatggcaac acttgaagaa ctgcttgacc aacccctttt
    12001 tgtaaaagaa gtcaaagaag catttgcaaa agcttttgaa aagctgtatt ctgcaagtga
    12061 tttacaggat tctaccgact ggctaaaaaa agaaatccag ctggagcaat ggaacaacca
    12121 aatccagaga atggaacaac gtaataacat aaaggagcta cattatgaca accccttata
    12181 aatatcaaat gctgcctatg gaaaaagttt ttcgtgatcc agtccataac tatatccatg
    12241 tccagcatca agtaatctta gatttgatca attcgaaaga agtccaacgt ctacgacgca
    12301 ttaaacaatt aggcacttct tctttcactt ttcacggagc agaacatagc cgttttactc
    12361 attcattagg cgtttatgag atttcaagac gaatctgcga tatcttttct cgaaattttt
    12421 caaaagaaaa aataggcaac ggaggctggg atgatcagga acggctggtg actttatgtg
    12481 cggcactgtt gcatgatgtc ggtcatggtc cttactctca tacatttgaa catatcttcc
    12541 atacgaatca tgaagccatc actgtagaga tcatcacttc tccagaaaca gaagtgtatc
    12601 agattcttaa ccgagtagaa gaaggttttc ctgaaaaggt agctagtgtg atccaaaaaa
    12661 cttaccccaa cccacaagtt gtccaaatga tttctagtca gatcgatgca gatcgaatgg
    12721 actatttgct tcgggatgct tatttcactg gaacagaata tggaacattt gatcttaccc
    12781 gaatattacg tgtgattcgt ccttatgaag gcgggatcgc tttttccatg agtggcatgc
    12841 atgcagtgga agattatatc gttagccgat atcaaatgta tgtgcaagtg tacttccatc
    12901 ctgtttcccg tggtatggaa gtaattttgg agcatctgct tcatcgcgca cacgagttgt
    12961 atcaagaaga tgccagcagt tttgaactcc attctcaatt acttctgcca tttcttgacg
    13021 gaagctttac tttagaagaa tacctcaaac ttgatgatgg ggtcttgggc acttacttta
    13081 ctcaatggtc tgaatctgct gatcctattt taaatgattt agctaaacgt tttttgaatc
    13141 gtaaaccact gaaatctgct actttttctg gcaaccggga ttcaaaatta gtccaagaac
    13201 tcactttgtt agtagaaaag gtcggatata atcctgttta ttacactgct gtgaattcta
    13261 gctacgatct accttatgat ttttatcgcc cagaacaagg cagacatcgc acacaaatcg
    13321 agattttgcg aaatgacgga actttgatag aattatcaca ggttagtcag cttgttgctg
    13381 cattagcggg acaagaacaa ggagatgagc gatttttctt tccaaaagaa atggttgatc
    13441 cgtccttgcg tgatcattat gatttatttg acgaaactta tcaagaattt gccagccata
    13501 tccgtaatgg cgcattgatt gaaataaatt aaaatatatt ggaggaaatt atgtctatca
    13561 aactagtcgc tatcgacatc gatggtactt tattaaatga gaaaagagaa gtaacaaaag
    13621 aagtaaaaga agcgattgcc gctgctgttg caaaaggtgt ttcgatcgtt ttatgcactg
    13681 gccgtccctt accaggtgtc caggaacagt taaacgagtt gaaccttttt caagataatg
    13741 attacgttat tacttataac ggggcactcg tacaacaaac gaaatcaggt aaaatcatcg
    13801 cccgtcacgg actgacacat gaagatttcc tagagatcga agtgatggca cgccgtgtcg
    13861 gcagtcatct ccattcgatc gatgacaaag cgatctacac gcctaatcga gatatcagcg
    13921 cttatacgat ccacgaagca agtttagtca agatgccgct aaaatatcga acacctgaag
    13981 aaatgacacc agacatgaac atcgtcaaaa tgatgatgat cgacgaacca gaaattttag
    14041 atgcagcaat tgcgcgttta ccgcaaactt tccgtgataa atacacaacg gtcaaaagtg
    14101 cgccctatta cttcgaagta ctaaataaag aagcaagtaa aggtgcggca gttgcgaatc
    14161 tcgcacagca ccttggtatt gatcaagatg aaatcatggc tatcggagat aacgagaacg
    14221 acttatccat gattgaatat gctggtctag gagtagcaat gggaaatgcc gtttctcttg
    14281 tcaaagaagc agcaaatgtc atcacttcat caaacgacga gcacggtgta gctgaagcaa
    14341 tcaaa
//
LOCUS       DAYYGS010000066        14194 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000066 DAYYGS010000000
VERSION     DAYYGS010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.066"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..594)
                     /locus_tag="ACHA44_002012"
     CDS             complement(40..594)
                     /locus_tag="ACHA44_002012"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFM8905278.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            847..1122
                     /gene="rpsP"
                     /locus_tag="ACHA44_002013"
     CDS             847..1122
                     /gene="rpsP"
                     /locus_tag="ACHA44_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFM8905279.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            1134..1385
                     /locus_tag="ACHA44_002014"
     CDS             1134..1385
                     /locus_tag="ACHA44_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HFM8905280.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            1509..2300
                     /locus_tag="ACHA44_002015"
     CDS             1509..2300
                     /locus_tag="ACHA44_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905281.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            2470..2991
                     /gene="rimM"
                     /locus_tag="ACHA44_002016"
     CDS             2470..2991
                     /gene="rimM"
                     /locus_tag="ACHA44_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFM8905282.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            2981..3742
                     /gene="trmD"
                     /locus_tag="ACHA44_002017"
     CDS             2981..3742
                     /gene="trmD"
                     /locus_tag="ACHA44_002017"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFM8905283.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            3878..4225
                     /gene="rplS"
                     /locus_tag="ACHA44_002018"
     CDS             3878..4225
                     /gene="rplS"
                     /locus_tag="ACHA44_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFM8905284.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            4535..4777
                     /locus_tag="ACHA44_002019"
     CDS             4535..4777
                     /locus_tag="ACHA44_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HFM8905285.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(4865..5011)
                     /locus_tag="ACHA44_002020"
                     /pseudo
     CDS             complement(4865..5011)
                     /locus_tag="ACHA44_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
     gene            5068..5382
                     /locus_tag="ACHA44_002021"
                     /pseudo
     CDS             5068..5382
                     /locus_tag="ACHA44_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345994.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
     gene            5411..6670
                     /locus_tag="ACHA44_002022"
     CDS             5411..6670
                     /locus_tag="ACHA44_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905286.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            6723..7619
                     /locus_tag="ACHA44_002023"
     CDS             6723..7619
                     /locus_tag="ACHA44_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905287.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            7630..8499
                     /locus_tag="ACHA44_002024"
     CDS             7630..8499
                     /locus_tag="ACHA44_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905288.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIELFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            8509..9184
                     /locus_tag="ACHA44_002025"
                     /pseudo
     CDS             8509..9184
                     /locus_tag="ACHA44_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            9390..11054
                     /gene="alsS"
                     /locus_tag="ACHA44_002026"
     CDS             9390..11054
                     /gene="alsS"
                     /locus_tag="ACHA44_002026"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0034077 - butanediol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HFM8905289.1"
                     /translation="MAEKMKQGSKSVVERLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            11067..11777
                     /gene="budA"
                     /locus_tag="ACHA44_002027"
     CDS             11067..11777
                     /gene="budA"
                     /locus_tag="ACHA44_002027"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HFM8905290.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            11907..12404
                     /locus_tag="ACHA44_002028"
     CDS             11907..12404
                     /locus_tag="ACHA44_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFM8905291.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            12589..12849
                     /locus_tag="ACHA44_002029"
     CDS             12589..12849
                     /locus_tag="ACHA44_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905292.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(13211..13450)
                     /locus_tag="ACHA44_002030"
     CDS             complement(13211..13450)
                     /locus_tag="ACHA44_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905293.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            13736..14050
                     /locus_tag="ACHA44_002031"
     CDS             13736..14050
                     /locus_tag="ACHA44_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905294.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         4732 a         2302 c         2879 g         4281 t
ORIGIN      
        1 tcctgtacat agttgccttt tttattaccg ttatgctttt tactctgtta atttgttaga
       61 aaaatctaga tacgcatcta aaatcgtttg gaagaaactt aatgttcctt caacgatatt
      121 accattttca tcaattagat tcgcaacgtt tccgatatat gcttccggtt gctgcaaggt
      181 tggcacattc aaaaagacaa gtgattgtcg tagatggtgg tttgcgccaa agccgccgat
      241 cccacctggt gatgcagtaa cgactaaacc tggtttatga tcccaaacac tgtgtccata
      301 aggtcttgag cctacatcta aagcattttt caatactgct ggcacagaac ggttgtattc
      361 aggagtaacg aaaacgacac cgtctagtcc cctcacttcc tcacgaaaac gagtccattc
      421 agcaggagcc tgttctggag tttcgagatc ctcattgtaa aatggcagat catcgatttt
      481 tacaaataca ctttcgtacc cttcaggaag caatttcgca aaagcttctg ctactttttt
      541 gttgtaagaa tcttttctta aactaccaat aaaaaatcca attttttttg tcatacgatt
      601 tttcctcctt caaactatct ataacaatct tacaaaaaat aagtaactgg agcaaataaa
      661 aacacttact gacaaaagct acatagaaga tagattactt atttttaaca aaaaagaagt
      721 ctaaaatctt ctatttccta aaaaaaatcg aatttaatca agctgtaaag aaaaaaagct
      781 ttacagggta aaaaaaaact gttacactac atcttgtgaa taaaacaatg gaggtggaaa
      841 taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt ctaaaaagag tccgttttac
      901 cgtatcgtcg tagctgattc acgttctcct cgtgatggac gtttcatcga aactgtaggt
      961 acttacaatc ctttgaaaga ccctgcagaa gtagttttaa aagaagactt agttcttgac
     1021 tggttatcaa aaggtgcaca accttctgac actgtacgta acatcctttc aaaagaaggc
     1081 gttatgcaaa aacaccacga agctaaattc tcaaagaaat aaggtgaccg gatatgaaag
     1141 atttaagcga cttagtctta acaatcgttc gtccgctagt cacttatcct gatcaagtca
     1201 agttagacgt cgtagagtcg aaagactttt acgagtacaa tctaacagtg gctcctgaag
     1261 atattggccg tatcattgga aaacaagggc gtgttgctaa agcaatccgc acaatcgttt
     1321 acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa tatcatcgat gataaggaaa
     1381 agtaaataaa cgtgctgtac tcgaattttc gagtgcagct tttttgtttt tttattaatt
     1441 gaagaaatga ttatttggca aaacaagcaa gaaagcgtat ccttatagga ggagggagtg
     1501 aacagccaat gagaaagaaa atgttattta ttccatttgc tctattgtta agcgcttgta
     1561 gttcggttac tcaagaatca gacaatacac atctatcaag cagtatatct gaaactgtta
     1621 ccagtccaga aaaaagtagt accaaaacgg ctaccaccac tgaacaaaca atggctacct
     1681 caaataatga gaaaaagact gctttagacc agttgaaaga acaacagcca aatgttccca
     1741 tgcctctaga tgtaccagtc tcttcaggct atctcaatat cgcagcgaca catacaaagc
     1801 aaggttattc tattttatat tacagaacag atcgtcccct cgggttgaat gctgacgaat
     1861 tgaatcaaga aacgcctatt gcaacctatc tatatcaata tgggtttgct tctagtcaag
     1921 aaacaatcca agttttgcag ccctttgaga tcgacacaaa tggtcagcaa gtggacttag
     1981 gttctcgaat cacagggtat cagcaaggtg cagcaggttc aagttttctt gaatggcaag
     2041 aaggaaactg gcgtattcga atccgtggga ataatataga aggccaagat cccttactat
     2101 tagctaaaga aatcgtagct tatttggaag aaaacagtct accagcacct gaacaatttg
     2161 gtaaaattac tgttgatatg ggtgatacga cgaatcgagc cgtagaagta agctggcaag
     2221 aaccgaaaaa tgcctatacg atcactcatc aagatcctat gtctgcgttg aagatggcag
     2281 tttctatgaa gaggttgtga aatcagcgcg ctgatctgag cccatcattt aactccaagt
     2341 cacaggaagt aatcaaaaat agcacgccgc atttttgcat aattttggct taatacctta
     2401 aaagaaaact agaattgcct cttcacacat gctaaaataa aacagacaaa aaagaaatgg
     2461 ggatataagt tgactgaata cttgaatgta ggaaaaatcg taaacacaca aggtatcaaa
     2521 ggcgaagtcc gtgttatctc aacaacggat ttcccagaag aacgttacaa aaaaggaacg
     2581 gtgttaacac tattccaaga cggaaaagca cctgtggaat taacagtcaa aagtcatcgc
     2641 aaacataaaa attttgactt attgagtttt gaaggacatc cttctatcaa tgatgtggaa
     2701 aaatatcggg atggcatatt aaaagtgtca aaagacaact ctgtagatct agctgaaaat
     2761 gaattctatt accatgagat catcggtctg catgtattag aagatggaac tgagttaggt
     2821 aaagtaaaag aaatcttgtc accaggtgcc aatgatgttt gggtcgttca acgtccgaaa
     2881 aaagctgata tattgcttcc atatatcgct tctgtcgtca aagaagtcga tttagaagct
     2941 ggagtggtcc gtgtagaaat tccagaagga ctgattgacg atgaagattg atgtattgac
     3001 tttatttcca agaatgtttg aaggtcctat gggggaatcc atcatcggta aggctgtaga
     3061 taaaggatta ttagatatca atatctctaa cttccgcgat tattcagata acaaacatca
     3121 gacagtagat gattatccat acggcggtgg agcaggtatg ctgctcaaag tccagcccat
     3181 ctatgataac atccaagcca ttgaaaaagc tgcaccggat gtcaaaaaaa gagtaattct
     3241 tcttgaccca gctggtaaac gattcgatca gaaaatggca gaagaatttt cacaagaaga
     3301 acacttagta ttcatttgtg gtcattatga gggatatgat gaacgtatcc gcacattagt
     3361 gacagatgaa gtttccttag gagattatgt ccttactggg ggagaattag gtgcgatggt
     3421 catgatcgat gcaacagttc ggcttttgcc agatgtgtta gggaatcaga cttcagctca
     3481 aacggattct tattcaaccg gtttattgga acatccccaa tatactcgcc cggcagaata
     3541 taaagggatg aaagtaccag aagtattgac aaatggaaat cataaattga ttgaagaatg
     3601 gcaattaaaa gaatcactaa gaaggaccta tcaacgtcgg cctgatcttt tggaaacact
     3661 tgaatggaca cctcaaatga cgaaattttt agaagaaatc aagaaagaag aacaagaaaa
     3721 aacagcagat gaagccaagt gactttttct aaaatattct ttacagtagg ttgataatat
     3781 gttagtattt tatggtgagt gcaaaagcac ttaactatta cgatattccg ctatgagaaa
     3841 agctcataag aatatttgga ataaggagaa gaaaaccatg aatccattaa tcgaagaatt
     3901 aacaaaagaa caattacgtt ctgacatccc agcgttccgc cctggtgaca ctgtgcgtgt
     3961 tcacgcaaaa gttgttgaag gtactcgtga acgtatccag ttatttgaag gtgttgttat
     4021 caaacgccgc ggtgctggaa tcagcgaaac ttatacagta cgtaaagttt ctaacggagt
     4081 gggcgttgaa cgtacattcc cattgcatac accacgtgtt gcaaaaatcg aagtagttcg
     4141 ctacggtaaa gtacgtcgtg caaaattgta ctacctacgt gcattacacg gaaaagctgc
     4201 tcgtatcaaa gaaatccgtc gttaatcatt gttatcagac tgagaaagaa cccttggtat
     4261 tcaagggttc ttttttctta gcatgtaatt tgaacaccct gatccagttt ttttctttaa
     4321 ttattggtag gggagaataa tggattagaa taaatctacc tataagtagt agtaaaaaaa
     4381 agagcactat gataatatct ttcttaagag taaccgcttt aataaaaaat ccagaatatt
     4441 ctaacataaa tggagtattc ttagaatcaa aaggagaaag aagatggttt atacttatac
     4501 ggagcaagaa aaaatgaaag tttgagacac attcatgaat gatggaaaag taaaacaagt
     4561 accaagttca acaaaaaaga aaaacatcct attgaaagaa gtcttgaaac gctttgacca
     4621 tggagtgact tatacagaaa cagaggttaa ttcgatactg ctgaacgtat tttctagtgg
     4681 cgattatgtc gaacaaagac gttatctaat cacatttggc tttttcaaaa gaagttcaga
     4741 cggtcgggca tatcagatga tgggaattga aaattagaag ttttaattta tactcattga
     4801 aaaaagttca agtatgcgat attggttcta caccgcatac ttgaactttt tttgtcatct
     4861 gtatctataa tacaaagcca agaacgttat tcaaaatatg gatagctatg ataaaagcta
     4921 atgagcgatt cgcagttaaa taagcaatcg tcagtaaaat cgttccagga gcgtacatga
     4981 aaaaatcaat tggatgcgtt acatgaataa agataattct cttttatttg tacgaataaa
     5041 taccaaaacg tacctcctaa aattagattc aaagaagata gcaaagagga aaaagatagt
     5101 tctgcagcag ctatagcggc atgtggattg ctagaacttt ccaaacaact gcctgtttca
     5161 gatcctcgtc atttcgatta cgaagagata gcaatcaaaa tcttggaaga attacagaaa
     5221 aactatacaa cgaaacgagt taacggatca aacggtctgc ttctccatgc cgtttatgat
     5281 aaaaacagct taaaaggtgt agatgaatgt gtgatctggg gcgattactt ttatgtagaa
     5341 ggaatcactc gtctagctaa aacatggtac tgttactggt gatgtataag agaagaggag
     5401 gattcatata atgaagcgat ggaaaaaaat gatgattgga tcaatagttt taggaatgtc
     5461 actcgtattt ggtgcttgtg gtaatcaaga agacagtagt gaagaatcag caaatgaaat
     5521 taccgtttcg acatggaatt atgaaacaac acctgaattc aaagcgctct ttgaagcatt
     5581 cgaaaagaaa acgggaatca aagtgaaagc agtagatatt gcttcagatg attatgatac
     5641 gaaactcacc acgatgcttt cttctggaga cacgacagat gtattgacga tgaaaaatct
     5701 tttgtcgtat tcaaattatg ctttacgaga tcagttagtc gatcaaacag aacgaattag
     5761 agaattagac actgatgctg ctgaagaaac atatgaaatg tatgatatcg atggaaaaac
     5821 atatgcattg ccatatcgaa cggacttttg ggtactatat tacaataaga aaatgtttga
     5881 tgatgcagga atcgaatatc caaaaaatct aacttgggac gaatatgaag atttagcaaa
     5941 aaaacttagt aaaaatgacg gacaagttta cggagcatat caacatatct ggcgttcgac
     6001 aatccaagct attgcagctg ctcaaaacga ggcgaatctt gttgaaccag attatcaata
     6061 tatgactgat tattatgatc gagcattgag gatgcaaaaa gaaggcgcgc agatggattt
     6121 tggcacggct aaatcaacga aagtaaccta tcagtctcaa tttgaagaac aaaaagcagc
     6181 aatgatgtac atgggaactt ggtatatggc tggcatactc gcaaacaaag aagcaagtaa
     6241 gacagaagta gaatggggaa ttacagctat tcctcagaag aaaaaaggag aatcggtcac
     6301 gacgtttggc tcaccaacag catttgctgt caataaaaac tcaaagaaac aaaaagcggc
     6361 gcaagaattt attgaatttg cagccagtga agaaggagca aaagtattag caggagtagg
     6421 agtagttcct tcatatcgta cggatgagat tgatcagctt tattttaatc tcgctggaat
     6481 gccaacggat gaaatttcta aaaaagcttt ttctcctgat gaaatcaaac ttgaatttcc
     6541 gattgatacg tatgggcctg caatcgataa gattttacaa gaagaatatg acttagtttt
     6601 ggttggagat gaaacgccgg aaaaaggaac ggtaaatatg gagaaacgtg tagctgcgga
     6661 aaaagaataa tcatgaaagg agagaagcca agagataaaa cactcttggt ttcgtcatcg
     6721 aaatggagaa tagtataaat aagaaagcta caaatcattt gcgtagaaaa cgaacgtgga
     6781 tcgcttattc gtttatcgcc ccgaatttca ttggtttttt cgtttttaca ttaataccaa
     6841 tcctgttttc gcttgtacta gcatttatga aatgggattc tttttcaacg ccagaatttg
     6901 ctggactaga taactttcgt aaaatggcca acgatgagac attttggatt tctttgaaaa
     6961 atactttttt atataccgct gctgtagtgc ctggcacact tgtttgttcg ttagggctag
     7021 ctttactact aaatcagaaa attagaggga tgaagctttt tcgtacgata tttttctttc
     7081 cgtatgttac ttccttagta gcaattgctg ttgtttggaa tatgttgttt catccaacag
     7141 tgggacctat taatatcttt ttaagacgat ttattgaaca tcctccaggt tggacttcaa
     7201 gttcaaattg ggcgctttgg gcaattgtaa ttgtcagtat ttggcgaggg atgggttatt
     7261 acatgattct ataccttgca ggactacaaa gtatccaaaa agaactatat gaagcgtctt
     7321 cgctagatgg agcaaaccgt tggaaacaat ttttgaatgt gacattacct tcgttgagac
     7381 caacaacctt ctttgtaacg atcatgcttg tcatcaattg tttcaaggtg tttgaccttg
     7441 tgcaagtgat gacggatggc ggccctggtc gagcgacaaa tgtgctggtt taccaaatct
     7501 acaatgaagc attcaaaaaa tttgattttg gttatgcttc agctatttca cttgttttgt
     7561 tcgtaattgt tttgggcgtt acggttgttc aattttcttg gaataagcgt cgtacttagg
     7621 aggtgtgata tggaagagag tgttcgaaat gaaagaaaaa aagcaaaaat tcgttcaaaa
     7681 aacatgccct ttattcaatg gttgggtatg attctgctga ttgtattagc tttattgacg
     7741 atcattcctt ttttatggat ggtttctgct tcattcaaag caaataatga agtgttaacg
     7801 attccgattc aatggatccc taaaacttgg catttagaga attatctggt tatttggtct
     7861 cgtattcctt tagtgacatt ttttaagaat acagcttttt taagcgttgt gatcacgttg
     7921 attcagttgt ttacttctag ttttgctgtt tacgggtttt ctgaactaaa ttttaaaggg
     7981 cgcgatattt tatttttagc atatatcggc acgattgctg tcccttggca gtcttatatg
     8041 atcccgcagt tcattatgat gagaaaagtg ggtttaacag atacgttgtg gtcgttggtg
     8101 ctattacaag cgttcaatgc ttttggtgtg ttcttgttga agcaatatta cagtagtatc
     8161 ccagattctt tatgtgagag tgcgcggatc gatggcttat cagagtggga aatctatcgc
     8221 aagatcattt tacctcttac aaaaccggcg attgccagtt tgacgatcat cacattcgtt
     8281 aatacatgga acgattatat ggggccattc atttatttgt cttctacgga aaataaaaca
     8341 atccagttag ggttgaaaat gtttattgag ctatttgatg cagagtacgc attaatcatg
     8401 gctgcctcag ttgtttctgt tcttcctgtt gcttttatat ttttagcgat gcaaaaatat
     8461 tttgtagaag ggatcgcttc atcaggaatg aaggaatagg aggtgattat gaaagagcag
     8521 cgttttgaca aaaatgttta tatggaattc ttttattggt tgtatcaatc attggcaagt
     8581 agcctttgtt tttggtgtgt caatatcttg tatatagcag ctgcgttcct tttgtcgata
     8641 gatggaagga atgtcttttc ctttattttt tctttacttt ttattggtcc aggttgcatc
     8701 gtgatgatag gtatgacaga agaattagca aaagaaaaaa gtttttcacc agtacgtttg
     8761 ttcataaaga cgtttcgcca attttggcta aagggatttt tttattggct atttgcttgg
     8821 atagttagtg tgataatgat ttttgattgt ttctttttta tacgtttttc ttatggaaaa
     8881 tggttgattc cgttgtttgt tttacttgct tgttttactt gcttgtttat cagtttcctt
     8941 tagcatcaac tgctggtatt ttcaggtgag aaatccagcg tcgaaaccaa atcaagttct
     9001 acggatagcg ttctactata cgttgaaaaa atggtatgtg agtttactag attttttact
     9061 attgacaagt ttgtttctct tctttttcgt aaagccccaa tggtgtattc tactaggtcc
     9121 ttctatcgta tttggtttga tttacttcaa caatcgtaaa ttgatgagaa ctatggatct
     9181 gtagacttta taaattgaaa ctttcaatta agaatgacag ttttttatgt aaaaactgtc
     9241 attctttttc attttttgaa acatatggct aaacaaatga aataaaacat gctatacttt
     9301 tgaatgaaac aggtttagaa actgtatcac ttatttgtga ttatttctaa ctaataatta
     9361 aaaataaagc ataaaaagga cgatgttcaa tggctgaaaa aatgaaacaa ggttcgaaat
     9421 ctgtagtaga aaggttgatt aatcatcatg ttgattatgt attcggtata ccaggtgcaa
     9481 aaatcgatgg tgtatttaat gaattagagg atcaaggtcc tgaattaatc gtgactcgtc
     9541 acgaacaaaa tgctgcattt atggcacaag caatcggacg tattacagga gagccaggag
     9601 tagttatcgc aacaagtggt cctggtgcta gtaacttagc tacaggtttg gtaacagcga
     9661 cagcagaagg tgacccagtt ttagcaattg ccggacaagt aaaacgaagc gatctattga
     9721 agttgaccca tcaaagcatg gataatgcag cactgttcca gccaatcaca aaatacagtg
     9781 cagaaatcca agatccagaa acaatttcag aagtgattgc caatgcttat cgtatggcta
     9841 agagttcaaa aaaaggcgcg agctttatca gtatcccgca agatgtagtg gatgcacctg
     9901 tccaaggtaa tgtcatcaag ccattgagcg atcctaaatt aggttctgct tctgcagatg
     9961 atatccgtta cctagctgaa cgcattagag aagcaaaact accggtacta ttagtaggta
    10021 tgcgaggatc aagtgaaaaa gaaacgttag ccatcaggca attagttgaa aagacagcat
    10081 tgcctgttgt agagacattc caagcagctg gagtgatctc acgtgaatta gaagcgcatt
    10141 tctttggtcg tgtgggttta tttagaaacc aaccaggaga tatgcttttg aaacgaagtg
    10201 atctagttat tgcaattggc tatgatccaa tcgaatatga agcaagaaac tggaatgcgg
    10261 aaaaagatgc gcggatcatt gtgatcgatg aagcgccagc tgaaatcgat ccttttatgc
    10321 agcctgaaag agaattgatc ggtgatatct cagccactct tgatttattg acaggttcat
    10381 tagagcctca acaagtgtca gaagatgcaa aagaatattt agcatctctt caagcaaaat
    10441 taacggagcg ggacattgtt caatcaaaag gagaagcagg tatcttgcat cctttagaag
    10501 ttattaacac gcttcagtca aaagtaacag atgatatgac tgttaccgta gatgtaggta
    10561 gtcattatat ttggatggca cgccacttta gaagttatga acctcgtcat ttgttattca
    10621 gtaatggtat gcaaacgctt ggtgttgcat tgccatgggc gatttctgct gcattggtgc
    10681 gtccaaatac acagatcgta tcagtttccg gcgatggggg atttttattc tctgcccagg
    10741 atttagaaac ggcggtacgt aaaaaattga atatcgttca tttgatttgg aatgatggac
    10801 actacaatat ggtagagttc caagaaaaaa tgaaatatca gcgggcgtca ggtgtagact
    10861 ttggtccagt tgattttgtg aaatatgcag aagcttttgg cgctaaagga atccgagcta
    10921 caagtgtaga ggaattagaa aaagctttag aagaaggatt tgcgacagaa ggacccgtaa
    10981 ttattgatat tccgattgat taccgtgata atgagaaatt gggagaaaca atcttaccag
    11041 atcaattcta ttaaggaagt gtcgacatga ctgaaaaaat cttataccag cacgggacat
    11101 taggagcttt gatggccggc ttgatggatg gaacagaaac gattgctcat atattagaaa
    11161 aaggaactct gggattggga acattgcatg ggcttgatgg tgaagtcatc tttttagatg
    11221 gcgtagctta tcaaggaaga tcagatggct cagttgccca attagatgga agtgaactta
    11281 ccccctatgc agcaattaca gattttacgc cagatactag tttttctgtc tcaaaaactg
    11341 cagatggaga acaattgaaa aaagacatat tagtaagaga agccggggaa aacctatttt
    11401 tggcagtgaa gataactgga ttattcaaaa atatgcacat ccgtatcatg ccgaagcaag
    11461 aaaaacctta cagaagacta gcaaaaattt cagagtctca accagaattc caacaaagca
    11521 atgtccaagg aacattagtc gggttcttta cgcctgaatt attccaagga gttgctgcag
    11581 cgggatttca cctgcatttt atcgatgata cacatacatt tggcggacat gtaatggatt
    11641 ttgaagtggc tgaaggaaaa gttgagatta gccgcatctc gtcccttgta cagcattttc
    11701 cagttgatga tccgactttt gttcacgaaa cgattgatta tgctgattta gcagcagaaa
    11761 tccaacaagc agaatagaaa aagacagcga gatgcgttca aggtgattaa cctgacgtat
    11821 ctcgtttttt tgaggagaaa acctagcgtt tacaaaaaaa ctgagctata atcttctcca
    11881 tagatcaaaa aggagcggtt tttatcatgt ggacagaaaa acagaaaaaa gatgcatacg
    11941 aaattttatt attacagcaa caaggattat tagaagcaga agataactgg ttagcaaatt
    12001 tagcaaattc atctgcattg ttgaatgaga ctttgcctga gactgtcttt gcaggatatt
    12061 atttattcga tggtaatgaa ttgattttag gaccgttcca agggcgagtt tcttgcacac
    12121 gtatcaagat gggaaaagga gtttgtggag aatcagctga aaaacaagaa acattgatcg
    12181 ttgatgatgt caaaacccat gcaaattaca tttcctgcga ttcagccgca caatcagaaa
    12241 tcgtagtgcc aatggtaaaa gaaggtaatt taatcggtgt tttagatatc gattgcggag
    12301 taacgaaagg gtatgatgaa atcgatcaag catttctaga aaaatatgta gatcttttat
    12361 tagcaaattt taatataaat gctttttcat ttattaataa ctaaaatcgt ctattgaaac
    12421 aaaaaagaag agaaatcaag aataaaaaga gccggaaaga cagtgggcat ttctgttttt
    12481 cggttttttt atttttttat aaaatcacaa gtgttattga ttttcataat catattttaa
    12541 tgttattatc attttaaata atgaaaaaac attaaggatg gatgaaatat gaataacgaa
    12601 actatcattt taggaaaaaa atatatttgc caacctattg gcttgaaaca aaaagtgatt
    12661 ggagaagtaa tcagtaaatt agaaaattgt gttgtgatct gcgtagacga atatcatgac
    12721 gtcgatcgca atgaaatatt agaaaaatgc ggaaaagtag tggcaaaata tactcacgtt
    12781 tatgagttgg aaacaacaga atatctatat aactatagca acaaatatga acatacttta
    12841 gtttcataaa gatttaaata aagtgccttc aaaagcgaga agaccaagct tttgaaggca
    12901 ctttatttag ttggcagtga ttgttcatga tttttgattt attcaaaagg ttcgcctaga
    12961 gaatctgtct ctgtaacgag gcctggtgga gaaactcgat aaaaacctat actgccagaa
    13021 ctgtcctatt ttcggataga caaactagta gttttaaaaa gatcatccga tccaatcatt
    13081 tcaaaaaatg caaaactgat atcaggatca tttagctcag gcttgttatc gtaaagcatt
    13141 tgtaaaggtt gcatttagtt aggagtaaac gcgaaactat catcattatt tgctgaagaa
    13201 gatgaagaag ctaattcaga gtttgtttga aataaaggag acgtgctgac agtttcttta
    13261 ccctgttttt tgcagaggta gaaactttta tatcggaagt ttctaccgga gaatttttct
    13321 catttgtaga aattgttgaa tcgatactat tcgaaatttg cgtatctgaa ggtatgttct
    13381 ttgatgcaga agtcccacaa gcagtaagta acaataaatt tgataaactg ataaacagaa
    13441 ttcttttcat tatgtacaca ctccttatag tatcgtttag catacaccta tgctcagtat
    13501 taggcaaaat caatgcaaaa ctatcaagtc ttttatagta tctattagtc ttagtaatac
    13561 atatatggag agggtgatat cccagttcaa accagataca ttggttacca tctgtattaa
    13621 cgattatgcc tctaaacgct ttttttatga aaaaatttat aagtgaaaac gtaggtatga
    13681 tattatctag aggtaatttt tttgggtatt taaatagttg ctaggaggat agcttttgga
    13741 tttttacagt aattttatct taataatagc aatattatta cttctaaata tttggttttt
    13801 tgataagagt agaaatgcag ggataggttt tagaaccaaa cgaagtacgt cttctgaaaa
    13861 aaagtgggta tattcacaga ctatttttta cggaggggtt atttcaatca gtttactttc
    13921 ttcaacactt tattctttta atgttataga cgtttccatg tcaaatttta tatctattat
    13981 tggaatcctt atttctgcaa ttattacaca actacttctt gtgtttgaag agaagtcaaa
    14041 aaataattaa agcaattatg ccttttagta caggaattat tacttcttac aaatgtatag
    14101 cgaatgtgaa gcctgctgtt ttaattgttc tagaaaaact tttgagtgaa caattatttt
    14161 atgttaggat gaattaattt ctctagagtt tata
//
LOCUS       DAYYGS010000067        14071 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000067 DAYYGS010000000
VERSION     DAYYGS010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.067"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(142..1344)
                     /locus_tag="ACHA44_002032"
     CDS             complement(142..1344)
                     /locus_tag="ACHA44_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905295.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1347..1541)
                     /locus_tag="ACHA44_002033"
     CDS             complement(1347..1541)
                     /locus_tag="ACHA44_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8905296.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1588..2436)
                     /locus_tag="ACHA44_002034"
     CDS             complement(1588..2436)
                     /locus_tag="ACHA44_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HFM8905297.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2641..2991)
                     /locus_tag="ACHA44_002035"
     CDS             complement(2641..2991)
                     /locus_tag="ACHA44_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905298.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3169..4032
                     /locus_tag="ACHA44_002036"
     CDS             3169..4032
                     /locus_tag="ACHA44_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905299.1"
                     /translation="MFGLVQPNKVKVGQRIKEIKDGMSLSFTELGNRLGLKKPTISSY
                     VQGYALAPKSVINQLSSISGKPVGWFYFGHVEEYIREYLFLIGQKKIVDDYPEIIEKI
                     KQEFYTGEFKNPAWENELGYPLEEFIQECFVEYSSEIMKDYVVQITKEVLEESKTLNK
                     LSSKAKEEAVTILSSGIIDYIEMSGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSY
                     LIGKLINILGTDEDTELFLSSLSMIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREK
                     TPDEFYDWFEK"
     gene            4656..5648
                     /locus_tag="ACHA44_002037"
     CDS             4656..5648
                     /locus_tag="ACHA44_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905300.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5664..6548
                     /locus_tag="ACHA44_002038"
     CDS             5664..6548
                     /locus_tag="ACHA44_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905301.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6597..8204
                     /locus_tag="ACHA44_002039"
     CDS             6597..8204
                     /locus_tag="ACHA44_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905302.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8205..9689
                     /locus_tag="ACHA44_002040"
     CDS             8205..9689
                     /locus_tag="ACHA44_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HFM8905303.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9866..10858
                     /locus_tag="ACHA44_002041"
     CDS             9866..10858
                     /locus_tag="ACHA44_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905304.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10910..11779
                     /locus_tag="ACHA44_002042"
     CDS             10910..11779
                     /locus_tag="ACHA44_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005472675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8905305.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12199..13812)
                     /gene="guaA"
                     /locus_tag="ACHA44_002043"
     CDS             complement(12199..13812)
                     /gene="guaA"
                     /locus_tag="ACHA44_002043"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFM8905306.1"
                     /translation="MRRTERAAYRTKFYSNLSSPTRFDEEPKIIVLDYGSQYNQLITR
                     RIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVL
                     GICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVT
                     QAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTG
                     DWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVL
                     LRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDE
                     ATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLF
                     KDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANA
                     GLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKI
                     SVRIVNEVAHVNRIVYDITSKPPATVEWE"
     gene            complement(13819..>14071)
                     /locus_tag="ACHA44_002044"
     CDS             complement(13819..>14071)
                     /locus_tag="ACHA44_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8905307.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
BASE COUNT         4307 a         2384 c         2908 g         4472 t
ORIGIN      
        1 tgaaaaacgt tgatttatcg gcattcccca tccatattat atttacgtct tattcccact
       61 caatatgagt agcctcgtaa agaagctatt atatcagtgc tttttcagcc tattcctcat
      121 tttggtaggg aaatggtagg gttagatttt tactgcttga tttccattaa agtttgcaac
      181 tttagatttt gttgcacagg taaatttaat gtttcctgtc atttgttctg caattccttc
      241 atcattacgg ctccatagat gagcgtagtg ctttaaagtg atttcagggc tagaatggcc
      301 taaccgttgc gacacgacca gaatgtctgc gttaaactca ttgattaggt agctaacatg
      361 agaatgacgt aatccttttc cttgtattag gggtacctta gctagtttgg cataacgctg
      421 aataattcgt tgaacagttg aacgatagag tgggaggtca gtgtagctaa gaataaattg
      481 ttctacacca tgattttttt gaataacttt ccactttttt agcacttcaa ttgtatcatc
      541 gtccagagaa atgatccgca agccgctttc agtttttgtg taaggtttcc gaataaaatc
      601 tttttgattt gtcatttcca aggtgtgatg aaccctcaac tttttacgtt tgaagtccac
      661 gtcattccaa ttaagtgcta atccttcgct tacacgaaca ccagtcatgt aatatagcca
      721 gatcattaaa aaacacatat gttcgtagaa atcatcttta taaatttttg ccaaaacttc
      781 ttcaaattct tcttttgtcc agtaaggaac gattgatttt ccttttggaa ttgcttttgt
      841 tcttttggaa atattctcat tcagaaattg cagggtgacc gcatagtcta atgatttacg
      901 aaacataccg tacataagtg aactgtatgc ttgtgagtat ccgctatcgt tcaataacca
      961 aattcgataa ttttcacaat ctcgcacatt gatttcacga agcttcttat caccgaaaaa
     1021 ttcagtaatc tgaatcaatc cacactcacg agaactccaa gtactttttt gtacgctagc
     1081 tttgtagtgt ggaacgtaag actgctccat aaattctttg taagttaaat cataattcgc
     1141 aaatccattt acaaccatgt attcattttt tatacgcatg acttctttgt gtgcttcacg
     1201 agctgatgag aatcgttttc ctagagaatc ttttgttcct ttcttctgga tcctctttcc
     1261 agtcacttga tctgttccta actcaactag ataagaaaag cgaccatttt tatcttgata
     1321 cacacaaggg tattttgttt tcatagttat tcctcctgtg aaagaggaac acctataatt
     1381 tcttcaatta cttgtcgggg tactacccca actcttttat tattataata tggatatccc
     1441 tgatttacca ttcttgtttt tgcttgtcgg attattgtgg tagcttgatg tgttttaaat
     1501 cctagagcct ctaaatcttt tcgacaaatt gtatttttca ttgtataaac tcactttcaa
     1561 agaaaacact atcattgggt aatagtgtta tttctttatt tttgtataat gaacggtact
     1621 ttcttgaaaa tccatcactt gttcaggact tcccaactca tttaaagcta aagcattgta
     1681 aaaaaatgga cgtctaacta tatattcgct tgtatatagt atatcttgat tcgtggaatc
     1741 atagggtttc tttaccatct ttttggtgac agttggtagt ttgagattcc gggacttgaa
     1801 aaaagctttt ttcttatgct ctttcaattc cagctcttta tcgaaatatt tgctgatata
     1861 gcgcccgcga ttttcgatac tgtctacatc gatcttgttg atcttaacga aaccctgacc
     1921 ccaaatcgta gttaaccggt taaatgagat aaaggggaaa tcaaacaaga taatatggta
     1981 gtggattgca ccgcgttgct gcttttccca cgtggcgaga tactttaaat ttgctttttt
     2041 agttttatag aggtcataat tcagtcgttt gataaatttt ttaaattcat tatttgcata
     2101 ctcaatgtct tgaatgtttt ctttgaaagt gagagttaaa aatttcgttt gattgtcaaa
     2161 attcgtgtct acaaggcggg caatctcaaa tcgcatactt tgataatgtt tctgtttgcg
     2221 ttttaaactg tcatattgtc cttgagcgga taattcatca tagttttttc ttttttcaag
     2281 agtaatatca ttttcataaa tccaagctaa tttttgtttt cgttccagat ttacttcgtt
     2341 ttcgatcgtt ttacgtttgg gagtaatgat tgcttcataa tcataaattt caatatacgt
     2401 gggagtttca atgattttct gattataagc ttgcaaaaat catcactcct tgttgcgtat
     2461 tcgattgtca aagaaccagt cattttctcc tgatgttgat ctataaatca agttaagggg
     2521 gaaggcctgc gtagcagccc ttctagccta tggggtgttg ggcggtctta acggacagaa
     2581 acggctctgt gggcgctgcc ccgccgtttc gtcctaacca cccaacactc catagtatag
     2641 ttagttttgt gtagtggtac cttgttttct gggaatcaaa gttgccgtat cagcggttgc
     2701 cgataaccca taactgacaa attgtccatt cgagcgtccc caaggtacaa tcaccaaatt
     2761 ggtaaatgta attatggcta aaccttgttg ggtaagttct tccggcgtga tcacaggatg
     2821 ttcagatttg actttgacac tgattttttc gtagcgaagt tttggtagga tacattcgta
     2881 gctccaacct agaatttcgc cctccttttg ccagcgagtg acattgacga cttggtattc
     2941 tttttcgagt gtttcttttg gtaaactgag atcggtaatt tttattggca tcacaattct
     3001 ccttttttat acgaaagttt atacgatttg attaaaaaat atagatggcc atctacgact
     3061 tgtattatat acgagaactt atacgtagtc aagagatttt ctaaaaaaat atatacgatt
     3121 ttttatacga gtgttataat gaaaaaaatg ataaggagga atttgaatat gtttggttta
     3181 gtacaaccta ataaagtgaa ggtgggccaa cgaatcaaag aaataaaaga cggaatgtct
     3241 ctctctttta cagaattagg gaatcgatta ggtttaaaaa agccgacgat cagttcctac
     3301 gttcaaggct atgcgttggc tcctaagagt gtgatcaacc aattatctag tatcagcgga
     3361 aaacctgtcg gctggtttta ttttgggcat gtggaagaat atatccgaga gtatttgttt
     3421 ctcattggtc agaaaaaaat agtggacgat tatcctgaaa tcattgagaa aataaagcaa
     3481 gaattttata cgggcgagtt caaaaatcct gcttgggaga atgagctagg ctatcctttg
     3541 gaggaattta tccaagaatg ttttgtagaa tattcgagtg aaatcatgaa ggactatgtc
     3601 gtacagatta cgaaagaggt acttgaagaa tccaaaacgc taaacaaact atcatcaaaa
     3661 gcaaaagaag aagctgtcac gattctttca tctgggatca ttgactatat cgagatgagt
     3721 ggtgagatca agtatggaga gaaagatcgc attgcgaaaa tggtgaaaga atcaataaaa
     3781 acctttgatg tgaaacaaga cctgacattt aatgattcct atttgattgg aaagttgatc
     3841 aatattcttg gtacagatga agatacggag ctgtttcttt cttctttatc aatgatattt
     3901 actgataagc atttttccac tcgatttggt ggggaagaat tagtcgaaat atttcaatcg
     3961 atgcgaccag cgttaatcaa actctatcgg gagaaaacac cagatgaatt ttatgattgg
     4021 tttgagaagt aaaataagaa aagtggctta gattacaagt tggtgaaaaa atttttttca
     4081 gcataacatc agtaatctat aaaggggttg ttgattatac tgtagaagtt aagtactcaa
     4141 gagatttttt gtacgtaaac aaagcaaaaa agggtgcagt ttcactagaa aatgtactct
     4201 tttttgtgat ttttcttagt tatagagttt ttttgaaact ttaaaaatga gaaaacatat
     4261 tgattttaaa gagtgtagac atagcttctt ttgctaaata gattaaactt actggtgagt
     4321 ggtcttgaac atacttttaa atcacatttt actgagtttt attggatttt ataattcatt
     4381 ggaaacctcg tacttaatct gtttttatct atcgactaga atctgtatat attccagcta
     4441 tcaatgaggc cgcagaaaag ggtgcttaaa aataatctgt tgtttaaaat attattatca
     4501 gaatacttag atacaaaatt ctggcacaaa gtaaaaaaaa tattgactta aaaaaatgga
     4561 tatggtaatc tataaacgaa aatagtggta acgtttccaa aatgcatctt cgttaaattt
     4621 ttattttttt aaacgaaaat ggaaacgttt ctaaaatgta ttgggaggag attgaattgg
     4681 aaaagacatt gggaccaggt ggtgagataa gggcggtaca acgggataaa aaaaatattt
     4741 cgaagtggga aaaattaaag aaacagttgc ggcgtgataa gtggatgtac ttgttgctct
     4801 taccaggtat catcatgacg tttatcttta agtatgtgcc aatgtacggt gctgtgattg
     4861 catttaaaga ctataatcct atgctaggaa ttttggaaag tccttgggta ggactagatc
     4921 actttaggga atttatcagt tcaccaaatt ttacgctttt gctgagcaac acattaaaaa
     4981 ttagtattta tggtttattg ttaggttttt ttccaccaat tattttagca ctgagcttga
     5041 atcaaattat caataaaaaa ataaaacaga aaatccagtt gattctatat gcgccgaatt
     5101 ttatctcagt agttattatt gtggggatgc tgtttttgtt cctatccgca aatggaccta
     5161 ttaatgggtt agtagaaaag gttacggggc aaccaattat gttcatgtct aatccagctt
     5221 atttcagaac gatttatatt ttatcgggaa tttggcaagg aatcggctgg tcatccattc
     5281 tttacaccgc aacgctggcg ggagtcagta ccgatttata tgaggcagca aatatcgatg
     5341 gggcaaatat tttacaaaag atttggcata tcgatttgcc tgcattgagg cctgtgatgg
     5401 tgatcagctt catcttggcc gccggcggta ttatgaatgt aggatatgaa aaagcctact
     5461 tgatgcaaac ttcaatgaac atccctgcct cagaaatcat ttccacttat gtatacaaag
     5521 tcggcttgca atcaggtgat tatgcctact ctgctgctgt tggtctgttc aatatgatta
     5581 ttaatgtcgt tttattactg attgtcaaca atataaccaa gcggctaaac gatggtcaag
     5641 gcttgtaatg aaaggagaaa attatgaata gtgctttatc aaaaatcgac cgtcaaatct
     5701 tatggttgaa tcgagtgatt gtaacttttt tagtcgtcat caccattgtt ccgctgattt
     5761 atgtactggt agcttctttt atggacccag ctactttgat caataaaggt ataagcttca
     5821 atccgaaaga ttggacaata cagggatatc aacgggtatt tgcagatgat tccattttac
     5881 gcggttttgt aaattcgttg ttttactctt ttagcttcag cttattgact gtatttatta
     5941 cgatgatcac tgcgtatcct ttgtctcgac ctaacttggc ggggaaaaag attattatga
     6001 ctttttttgt cattacaatg ttcgtgggtg gtggcttgat acctacctat ttattggtaa
     6061 agaacttggg catgttagat agtgtatggg cgattatcgt accaggtgct ataaatgtgt
     6121 ggaatattat cttagcccgt acctatttta gtgctttgcc aaaagaactt tctgaagctg
     6181 ctactatcga tggcgccaat gacttgcaaa tactgttcaa agtcatgctt ccattagcga
     6241 aaccaattat gttcgtattg tttttatatg cctttgttgg gcaatggaac tcatactttg
     6301 atgccatgat ttacatcaaa gatcccgact tacagccgtt gcaactcgta ttaagaaata
     6361 ttttgatcca aaaccaacct tcacaagaca tggtgggagc caatactgcc atggcagaaa
     6421 tgaaacaaat tgctgagttg atcaaatacg caacgatcgt catttctagt ctgccattaa
     6481 tcataatgta tcctttcttc caaaaatatt ttgataaagg tgttttagtt ggttctgtta
     6541 aagggtaagt attgttcact atttgtatga ataaaaatta taaaggagat tttaagatga
     6601 aagcaaaatg gatgctgcca atcgttttta cttccgtact aattttgggg gcttgtggag
     6661 gctcaaaaga aaagagtaaa tcaagccctg attatgaact agaaaatgtc tcttttccat
     6721 tgaaagaaaa agtatcttta aaaatgatgt ctcaaagtgc ccctttggct cccaatgatc
     6781 ctaatgacaa attgattttc caacgattag aagaagagac caatctccat attgattgga
     6841 caaactacag tagtgatttt gccgaaaaaa gaaatttgga tatcgctagt ggtgatttac
     6901 cagatgccat cttcaacgcc gctgcaggtg actatgattt attaaactgg gcggaaagtg
     6961 gcgttattgt accgattgaa gatttgatcg aagattatat gccaaatctg aagaaaattt
     7021 ttgatgaaaa ccccgaatac cgacaaatgt ctactgcacc tgatggtcac atttactcta
     7081 tgccttggat tgaagaatta ggcgaaggaa aagagtcgat tcatacagta aatgggatgg
     7141 cttggatcaa caaggagtgg ttggataatt tgggtcttga aatgccgcaa accactgatc
     7201 agttgatgac tgtgttggag gctttcaaga cgcaagatcc aaatggtaat ggtgaagcag
     7261 atgaaattcc ttttagcttt attaacaatg gaggaaacga agacttaaaa tttttaatgg
     7321 gggcattcgg tctaggtgat aatgatgacc acttagtcgt tgataacgat ggtcaggtac
     7381 aatttactgc taacaaagaa gggtataaag aagccatcaa atatttcaat gaaatgaata
     7441 ataaaggtct tattgataaa gaggctttcg aacaagattg gaatacgtat gttgccaaag
     7501 ggaaagaaca taagtatggc gtgtacttta cttgggataa agcaaatatc acgggtgcag
     7561 atgatagtta tgacgtcttg ccagtcttag cgggaccaga tggaacgaaa catgttactc
     7621 gtacgaatgg catgggtttc tcaagagatc gtttcgtaat tactagtgcg aataaaaact
     7681 tggaacttac agctaggtgg atagataaaa tgtatgaacc gttacagtct gtccaaaata
     7741 actggggaac ctacggtgat gaagcacagg cgaatatttt tgaattaaag gatggtatgt
     7801 taaaacattt gcctttagag gggacagcac cgggggaact aagacaaaaa accgaagctg
     7861 gaggaccatt agcagttctt gattcttatt acggcacagt cactactatg ccagatgatg
     7921 cgaagtggcg tttagatttg atgcatgaca cgtatttgcc atatattaca gaagacaata
     7981 tctatccacg tgtcttttta gaaaaagaag aaacagatcg attagcaaaa atcgaaacgg
     8041 atatgtggga ttacatctat cgcaaaagag cagagtggat caccggcggt aaaatcgatg
     8101 aagaatggga agaatacctt gctgaattgg aacgtgtagg tttggaagaa tggattcaaa
     8161 taaaacaaac tggctatgat aattttttga agggagcaca ataagtgtta gtagaagaaa
     8221 cggcgatttt ggataaagca aatcgattta ttgaaatgat gcgaggaaaa gttaaccctc
     8281 agtatcgaca aaactatcat ttttccgcac ccatcgggtg gataaatgac cccaatgggt
     8341 ttatatatta caaagggcat taccatctct tctatcaata ctatccttac gatagtcgat
     8401 gggggcccat gcattggggg catgcgcgca gtagtgattt ggtccattgg gaagatctac
     8461 cagtagcgtt agccccagat caaccttatg acagcggcgg gtgtttctca ggaagtgcaa
     8521 ttgaaagaga cggaaaacta tatttatttt atacaggctg tcaagaaatt gatggtatta
     8581 gccatcagca tcaatgtttg gcggtctctg aagatggtat tcattttagc aaatacagtg
     8641 gtaatcctgt ggtagcagaa aaacagctag gtgaaaatgg gctaatacaa gaatttcgtg
     8701 atcctaaagt aattgttcac aacgatggtt actatatggt agtagctact aaatctacac
     8761 agtctactgg tagaatagtg atgtttcaat cgtcagattt gtatacttgg cacttttact
     8821 ctgtgctgct agaagggact tcagagcaag gaataatgtg ggaatgtcct gatttattcc
     8881 atttagacgg acgagatgtc ttaatcatgt ctccgataca aatcaagaag aacggactgg
     8941 aatatcataa tatcagttct accatggctt gtattggtga ggtaaactgg gaaacaggtc
     9001 agatgatagt tgataatttc catgaaatgg attttggaat ggatttttat gcaccacaaa
     9061 caacaagaga tcctaatggt ggacgggtga tgattgcttg gatgcagatg tgggatcgta
     9121 cattgcctac acatgagtta ggccatcttt gggcaggctc tatgagtttg ccccgagagt
     9181 taagagtgaa aaaaaatcga ttggtacaaa aacccatctc aactgtatat agtcatttag
     9241 tttatcaaca aggcagtgaa aatattgcag tgactgacac atcagtcgta tttcgaaacg
     9301 cgctgtctga ttcttcatat ttacatctag tcgcagattt ggagaaagct gaagttttta
     9361 caattgaatt actgcggtct aagacggatt gtctcaaact agcctatgac actgctacac
     9421 aggtttttga attggatcgt cagaaactag gaagagagct tacaggggcg gaagaagacc
     9481 ctctgaatgt acgaaaagtt gaagtacctt tgattgataa tcagctcgtt cttgagattt
     9541 ttagagatac ctcatcaatt gaagtcttta ttaatgggtt agaagttatg acggctactt
     9601 tctatgagct ggaaaaagct cgagatatct tgtttcattc gatcggagaa agtcgtattc
     9661 aagcttttga aacagcgagt gtgaaataaa tgtttgattg cttaggcgat gaatcaatta
     9721 tttaataggt tttgaaggat aatgttttta gaaaagaaca gatagctaga tttcccatgg
     9781 atttgctatt agtattttga aaaaattagt aatttatgac aaaattcatt atactaaact
     9841 tgtgcagaaa tgtgagggat caactatgaa agcaacaatg aaagatgtcg caaaattagc
     9901 aggtgttgga gtaggaactg tttcccgggt attgaataac ggttctgtta aagcttctac
     9961 tagagaaaaa gttgaagcag caatgacaga gttaaactat caaccagatt tttatgcccg
    10021 ggggttaaag atgaagcata cgaacacggt tgctttgatt ctacccacgg tatggcatcc
    10081 attttttggc gaatttgcct accatgtaga aacagcatta gaaagacaaa actacaagct
    10141 ttatttgtgt aactccagtg gtaatagtga aaaagaacaa gaatatatcc agatggtcag
    10201 ccaaaataaa gtagacggca tcattggaat tacttattca gatatcgatg aatacatttc
    10261 gagtaatctg ccttttgtta gtattgaccg ttatttcact gaagaggtga tttatgtgac
    10321 cgctgacaat gcggccggtg gacgtttggc tgcaaatgaa ctttatcgcc gaggatgtca
    10381 gaatgtagca tatatcggtg ggtaccaaga aacaccgaat gaaacgaaga gtcgtcgaaa
    10441 atattttgaa atcgagtgcc gtcaactggg gatttcctat aaagcgttag atatgttaga
    10501 acctttagac catctctcag aacgtgtaac aaattttttg ttagagaatc ccgatatcga
    10561 tgggattttt acgatcaacg atccaatggc attagctgtc attgatatgc ttgaaaaaat
    10621 agggaaaaac gtaccagagg atgtccaagt tatcggattt gatggtcaaa agatgacaga
    10681 aggagatcct tatctagtct caacgattgc ccaacctaca aagcaaatgg cggaagtggc
    10741 agtggcaaaa ttaattgctg tcattaatgg agagacagta gaaaaacgta ccgtattgcc
    10801 gattgaattt ggcgaaggtg acacaacgaa acctttagtg agaaacgatt ttgcttagtg
    10861 caaatacgtt tctttttttg aaaatgagtg aatgaaaggt gtagataaaa tgttatatgg
    10921 tggaatagaa gccggtggaa caaagtttgt ctgtgggatt ggtaatgaac aattacaagt
    10981 gatagaacgg aaaagttttc ctaccacaac accagaagaa acgatggagc aagtgtttga
    11041 tttctttgat aagtacaaag gaaatatcgc atcaattggt gtaggagcat ttgggcctat
    11101 tgatattcaa atcgaatcaa gaacttacgg atatatcacc aacacaccga agttagcttg
    11161 gcaaaattat cattttgtag gagctttgga aaacgccttg tcggttccca tctattggac
    11221 taccgatgta aatgctgctt gttatggaga atatatggca gggtatggaa aaggtaaaaa
    11281 tagtgttctg tattacaccg ttggtacagg tgttggtggt ggtgctgtag tggatgggaa
    11341 aatagtcgca ggttttagtc atcctgaaat gggacatatg ctcttacaac gtcataatga
    11401 agatcaattt gaaggaatct gtccttttca tcatgattgt ttggaagggt tagcatcagg
    11461 acctgcgatt gaaaaacgct ttggaagaaa agcccagact atctcatttg atcatcctta
    11521 ttggacgatt gaggcagatt atcttgctca gtgtgtgtat aacacaacgt tactatatgc
    11581 accggaagta ataattttag gcgggggtgt aatgaaacag ccgcaactac tcgagttgat
    11641 tcgccaatcg tttaaagaaa aaatgggttc ctacgtggat acgcctactc tcgaagaata
    11701 tctactaact ccaaggcttg ctgacaatgc gggcttgatt ggctgcctag ctttagcaat
    11761 aaagaaactc aatcattaga atagtattta ttagaactat tcataacata ccggaagttt
    11821 aaaagggtat tctgaaactt aaatagtctc aattcattct tctttcttga gaatctgagt
    11881 agagactttt attctgtttc aaattaatat aattacgaaa tgatattgag tacaagaaag
    11941 ttctttttac cagatctttt aaatcgaaaa atctgatata tatatttata gtactaaaaa
    12001 tctgattaga taaaaagcta attcaatagg aaaataaagg tagtgaataa aagcaaagcc
    12061 cttgttagta gttaatctaa caagggcttt ggtagggaaa atggtaggaa accatcttat
    12121 tttttacgaa aatcaggaac tttagtaatt gaataatgtt gatttatagg tattcctcat
    12181 ccatattatg ttggctcttt attcccactc cactgttgca ggtggtttgc tggtaatatc
    12241 atacacgata cggttaacgt gtgcgacttc atttacgata cggacactga ttttttgcaa
    12301 gacttcccaa gggatacggg caaagtcagc ggtcatgccg tcaatagaag tgacagcacg
    12361 gatacctaca gtgtagtcat acgtacggcc atctcccata accccaacag agcgaatgcc
    12421 tggtaggacg gtgaagtatt gccagatgtc gcgatctaaa ccggcattgg cgatttcttc
    12481 gcgcaagata gcgtctgatt cacggacgat ttctaatttt tcttctgtca attcaccaag
    12541 gacacgaata cctaagcctg gtccagggaa tggttggcgc caaacgatgc tgtcaggcat
    12601 gcctaattgg gtacctaatt cacgtacttc gtctttgaac aatgtgttca atggctcgat
    12661 cagcttgaat tgcatgtctt caggtaatcc acctacattg tgatgtgatt tgatggtttg
    12721 ggcagtttca gttcctgatt cgatcacatc ggtataaagg gttccttgtg caaggaaaga
    12781 aacgccttct tcaccggcaa gtttggttgc ttcatcatcg aagacatata caaattcatt
    12841 tccaatgatt ttacgttttt cttctggatc agacacgcca gcaagttttg ataagaaacg
    12901 ttctttggca tcgactttta taatgttcaa gccaaatttt ccgccaagac tttccataac
    12961 ttgttctgct tcacctttac gcaacaatac gtgatcaacg aagatacatg ttaattgatc
    13021 gccgatcgct ttttgtaaaa gaacaccaac aacagaggaa tctactccgc ctgaaaggcc
    13081 taataaaact tttttgtcac cgacttgttc acgaattttt gcgatctcca tgtcgatgaa
    13141 gttatccatt gtccagtcac cagtacattg gcagacatct agtgtaaaat ggcgcaacaa
    13201 gtcatttcca tattcggaat ggcgaacttc tgcatggaat tggattccgt acattttgcg
    13261 ttctgcattt tggatcgatg cgatggggca atctttgctg gtagcaacga catcgaaatc
    13321 agtcggtgct tgggtaacta ggtcaccatg actcatccaa acggtttgtt tttcaggagt
    13381 gttttggaaa agggccgctt ctgcagaagt gatttccaat tctgcttttc cgtattcacg
    13441 gtttttggct ggtttaactt ttccgcctaa tttataagta atcaactgca taccgtaaca
    13501 gattcctaaa actgggatcc ccaaatcaaa aatcttttca tcaataccaa aggccttctc
    13561 atcataaaca ctgtttggtc cgccagaaag gatgattcct ttagggttca ttgcctttac
    13621 ttcttctgcg gtaatgcggt ggctcatcaa ctctgagaaa acaccgaact cacgaatacg
    13681 gcgagtgatc agttggttat actgactacc gtaatcaagt acgataattt ttggctcttc
    13741 gtcaaatcgt gttggtgaag acaaatttga ataaaactta gtccgatatg cggcgcgttc
    13801 tgtgcgccgc atattttttt agttttcaaa gatcagacct tgttgtaaat agatccgtgc
    13861 ccgaaagtta taaaagttgc gataaccaaa cgcaattcgt ttaatcagct tgatcttgtt
    13921 attcagcccc tcagtgacgc cgtttgagtg ggacacctta aaggcatttg aaatacccga
    13981 ttggtagcga tggaagatcg tgaatttctt tttaaacgac tgaggtaacg tagatgaaat
    14041 tgttttaagg gtttcttgaa agccttccgt a
//
LOCUS       DAYYGS010000068        14052 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000068 DAYYGS010000000
VERSION     DAYYGS010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14052
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.068"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            43..1188
                     /locus_tag="ACHA44_002045"
     CDS             43..1188
                     /locus_tag="ACHA44_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905308.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(1273..1578)
                     /locus_tag="ACHA44_002046"
     CDS             complement(1273..1578)
                     /locus_tag="ACHA44_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HFM8905309.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(1603..2034)
                     /gene="ruvX"
                     /locus_tag="ACHA44_002047"
     CDS             complement(1603..2034)
                     /gene="ruvX"
                     /locus_tag="ACHA44_002047"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFM8905310.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(2031..2297)
                     /locus_tag="ACHA44_002048"
     CDS             complement(2031..2297)
                     /locus_tag="ACHA44_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HFM8905311.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(2552..3277)
                     /locus_tag="ACHA44_002049"
     CDS             complement(2552..3277)
                     /locus_tag="ACHA44_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HFM8905312.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(3638..3708)
                     /locus_tag="ACHA44_002050"
     tRNA            complement(3638..3708)
                     /locus_tag="ACHA44_002050"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3674..3676),aa:Gly,seq:ccc)
     gene            3825..4577
                     /locus_tag="ACHA44_002051"
     CDS             3825..4577
                     /locus_tag="ACHA44_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8905313.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            4593..5072
                     /locus_tag="ACHA44_002052"
     CDS             4593..5072
                     /locus_tag="ACHA44_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HFM8905314.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(5323..6903)
                     /locus_tag="ACHA44_002053"
     CDS             complement(5323..6903)
                     /locus_tag="ACHA44_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFM8905315.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(6896..7639)
                     /locus_tag="ACHA44_002054"
     CDS             complement(6896..7639)
                     /locus_tag="ACHA44_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905316.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(7815..8591)
                     /locus_tag="ACHA44_002055"
     CDS             complement(7815..8591)
                     /locus_tag="ACHA44_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8905317.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(8839..9675)
                     /locus_tag="ACHA44_002056"
     CDS             complement(8839..9675)
                     /locus_tag="ACHA44_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HFM8905318.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(9686..10615)
                     /locus_tag="ACHA44_002057"
     CDS             complement(9686..10615)
                     /locus_tag="ACHA44_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFM8905319.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            10807..11160
                     /locus_tag="ACHA44_002058"
     CDS             10807..11160
                     /locus_tag="ACHA44_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HFM8905320.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(11234..11890)
                     /locus_tag="ACHA44_002059"
     CDS             complement(11234..11890)
                     /locus_tag="ACHA44_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HFM8905321.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            12116..14050
                     /locus_tag="ACHA44_002060"
     CDS             12116..14050
                     /locus_tag="ACHA44_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HFM8905322.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
BASE COUNT         4493 a         2740 c         2445 g         4374 t
ORIGIN      
        1 tcctatcaaa agcacgccac gcaactgata ggaggaaaaa caatggcaac aattaaacaa
       61 tatgaaaaga aagacggaac caaagcttgg atgtttcaaa cataccttgg aatcagcccg
      121 ctaactggta aagaagtccg tactacacgt agaaatttta aaacaaaaaa agaagctcaa
      181 atagagctta atagactact tgtagacttt gaagagaatg gacttaaaag ggaaacgaac
      241 gaaactttcc aagaagtgta tgatctctgg tatgaaagct ataaaactac tgtaaaagaa
      301 gtcactcaat taaaaattga gatcaagttt aataaatgga ttcttccaaa atatgggcag
      361 tttagaatta aagaaattac tacaaaacat gttcagaaga tattgaatca gtgggctaaa
      421 acaacagatc aatataaagt cctacattct accgtaagcc gtattttcaa atacgcaatg
      481 actctagggc tgatcactaa aaatccctgt gagctcgtaa tcatgcccaa cagaaatgtt
      541 gaaaaaaaga aggatactgt caaaatttat tcacgagatc aattagaaac cttatttcaa
      601 tatttggaat ccaaaagaag tacgtatagg gcagagtacg ataaaacttt actgcgcttt
      661 ctatttttta gtggctgtcg aatcagtgag gctttagcct tagactggtc agatattgac
      721 tttgattcaa acaccgtcac tataaacaaa accttaagcc aaacaaaata tgggtacaaa
      781 atttcaagtc caaaaacgag aaatagctat ggttccctat ctttagacaa taaaaccatt
      841 aattggctaa agaaatggca aatcaatcaa aaaagatata tgttaaccct cgggattact
      901 gatccaacaa tgattttttg tggaatctac aaagaaatca ttactaaaca cgctatctat
      961 tctcgtatga tcacaatctg caagaattct ggtattccat ttctaggtaa ccatgtaacc
     1021 agacacactc atgctagtat gctgttagag tcgggagtct ccatgaagga agttcaagaa
     1081 cgccttagac actcatcaat caaattaaca atggatacct atgctcatct aactaaagaa
     1141 accaaagaaa aaaccgttga aaagcttgca gagcacctaa atttctagcg gaacgtattc
     1201 aatcccgtat tcaattcaaa acaaaaaaaa gctgaaccct tatctaccaa gggttcagct
     1261 atcatttaca gcttattctt cttcttccgc catgaatgta ttgaatactt cttcaatcat
     1321 gtcccattct gcgtctgttt cgatttgttc taactcgcct tctgttccat cttcgttttc
     1381 gatgtatgca taagcttgta attctacatc ttcatcttct gaaacaccag caggatataa
     1441 aagaacatag ttacgtccaa attcttcttg tccgtcaact gttagtagga tttcatataa
     1501 tgtttcattt ccttggtcat ccaccaatgt gatgtgttcg tgtccttcat ggtcgtggga
     1561 atggttatgt tctgtcataa gattacctct tttcttttat tattaaaata attgcttacc
     1621 tgagccgtct aaataatttt gtaagatcat cacagctgct aatttatcga tgactttttt
     1681 tcgtttggct cttgaagtat ttgcctgctc tactagcata cgttctgcct gaacagtggt
     1741 taatcgttca tcttgataat caactggcaa ggagaatagt tctttgatct tttctccgta
     1801 tgacatggaa gcttctgccc ttggtccgat ggaattgttc atatttttag gtaatccaac
     1861 tacaaaccgg tctacttcat attgtgctac taattccttc aaccgatcaa agccaaactc
     1921 ttcttctgct tcattgatcc gaatgatctc tactccttga gctgtccagc caaaggggtc
     1981 actgatagca acacctactg tcttagaacc gacatccaag cccattactc tcataaattc
     2041 actccatgat ttgagaggta gtccttgaca attacttcca tgatctcatc gcgttcgtga
     2101 cggcgaatca aatttcgagc atcacgataa cgaggaatgt aggccgggtc tccagaaagc
     2161 aagtagccga caatctgatt gattggattg tagccttttt cttccaaagc ccgataaaca
     2221 atcgctaatg tttcgctcac ttcttttttg cgactatcgt caaaaccaaa acgtacggtt
     2281 tcatctgtaa aacccatgat tctacacctc tttcccgcaa ggtaattcca gcttaccttc
     2341 tacaacctta ttttactcga agaaagggaa aactacaaac aaatattcca aacttccgaa
     2401 taaattaaga aacactattt gtatgttttt ttcctttttt ctgtagcttt atcaaaagta
     2461 ctcttttcat aaagtatacg ctatatttct ttgaatgtat gtttttacac aattttttca
     2521 taatattcat aaaaaattcg aatagctgct actaattttt tgtttttgtt gcttgtcctg
     2581 tctcataatt gatcgtgtag ccatcttgaa cattgtagat aaagacattg aattcaattt
     2641 ttttgtcttc aatgctttct gcttccaatt gcactcccct ggctaccagt tcattcccct
     2701 caaaatgagg agtgacacgg tagcgtacat gatgatttgt ttctttgata tatgccgcaa
     2761 tatcattttc gtgagcaagc atgtatggag cattcaacga acgtgtccct gtcatcaagt
     2821 ttttaatgtt gttattttga ccagtcagct ggaaaccaat taaatggctg cgattatata
     2881 accattgccc gtcacttaat ttcttttgtt tccaacctgt cgggtcaata tacaaaggct
     2941 ctctcttttc agtagggaac aaatcttttc caagcatagc attggcttgt cctactctgt
     3001 tcaatttatc aatatttgaa tagctttccc atgttccttt ggaaaggcta agatcctctt
     3061 ttgtaaattc cggctgattc ccattgattt ctatttcttg atgttttcca tcatattctg
     3121 gcacatccgc tacttgagaa gcttgtggtg tggaatcaaa aatagttgta atctgctgaa
     3181 agatatctga atctttttgt gtggtggaaa caccgaatgc ccctgcaatc acagcagcga
     3241 cgatccccac gatcgccttg gttgtttttt tgctcatttc tttactcctt tactaacagc
     3301 tccctaaatc ttaacatatg ttcccctata aataaatact agaaagaaat agaaacagca
     3361 aaaaataaaa cctgttttaa gatggcttaa aaatacaaaa aagagacgga ttcctcgtct
     3421 ctaatcagtc accactctat ctgtaaggta tttattatta ttcaaaaatt ttcttcatac
     3481 tcaagacaat acactgattt cacaaccgct tggtaaacgg tgcgcaaaag cttgcatctg
     3541 cggtaataag tccatccaag ttaaaaacat aagaagttgt ttttgcttgg ctgttcttat
     3601 tacttgttgc agagcagctt tttcacaacc tattttaagc gggtgacggg aatcgaaccc
     3661 gcgacatcag cttgggaagc tgggatttta ccactaaact acacccgcaa tacaaaaaca
     3721 gtatacgcct cttttattcc ttcctcaagt ttttttattc tttacgcttc tggtatgttt
     3781 tgtagtaaaa taaaactaat caatcaatta ttggagaaga tgctatgacg ggaaaagaat
     3841 tagatctgtt gctggacact tgtttactcg ctgggaaaat catgatggaa agcaatgcgg
     3901 agatgtatcg ggtagaagat acgatgagtc gaattgcttt agcttctgga aatttccgtt
     3961 tggtcagcta tgtcactcaa accggtctct ttattggtct tgatcgtacc tcgaccatcc
     4021 gaatggaaca aattaccaac cgttcgatta atttagaaaa agtagtaaaa gtaaatgatc
     4081 tttcacgtaa atatgttgct tcagagctaa cattagaaga actttactgc gaactgcaag
     4141 caatcgaccg agaccgttct ttctttcctg tctggttgca aatcgttagc gcggcagtga
     4201 tcagtggaac aattatggtg ttatttggcg gaaagatgac cgatttacct gtgactttag
     4261 tcattggagg attgggatac gcagcttatc tatatagttt gaaatttttt agaatcaaat
     4321 ttttatcgga atttttatct tctttattga ttggcatagc cgctatctac agcgtacgtt
     4381 ttggcttagg aagcaatcag gatctaatca ttatcggatg tgtgatgcct ttagttcctg
     4441 gagttcaaat tacgaacgct atccgtgatt tgctagctgg acattatgtc tcaggagttt
     4501 ctcgcggaac agaagcgatg atgactgcta cgatgatcgg ttttgcgatt gcttttattt
     4561 tccagctttt ttattaggaa aggaacacaa gtatggggac gatatttatt cagttttctt
     4621 ttagtttttt agcgactgcg gcatttgccg ttattacaaa tgtacctagg cgttcattga
     4681 tttgttgtgg tctttctggt gcttttggct ggatgatcta ctggatctgt gttcaaatag
     4741 gcggatctgc tgcttttggt tcattgcttg gttctttagg agtagcattc atcagcgatc
     4801 ttttctctaa acgtttgaaa atgcccgtaa cgattttcaa tattcccggg atggttcctc
     4861 tagtccctgg tggtttagct tatcaagcgg ttcgaaatct cgttactgaa tcctatcgag
     4921 aagcggctca ttatgcggtc caagcaatta tgattgctgg agcaatcgct ttaggtcttg
     4981 tattatcaga agtctttaac cataacatcc gtaatttcaa agtaaagcgt gacgcaatca
     5041 tgatgaaacg aaaaaaacgg ctttttaaat aaacgaagtg taacaaagaa aaggttgtga
     5101 aagagacgtt cagccttgag cgatgacaaa atatctcaca aacattgcga caagtaaccg
     5161 caggagcaat aagaagtaat tatgagtatt aaaaaaagtt ttgtaaaaat agcttctcac
     5221 atttttacat aaaattgact taatactgag aaactgactt ttgaacaccc tctatctgta
     5281 aaaaaggggc tgcgacatga tttttgtcac ggcccctctt ctttattcaa attttttcca
     5341 aaacgttcgt tgataataaa cgatccatag agcggaaagt attagaacta aaccgatcac
     5401 aggaatattc actaaccaaa atggataaaa caaagccaac attccgtata agacgataag
     5461 aataatactg atgatcaatg aacccatcat agttgcgact aaacccaaac ttcctgaacc
     5521 tctgttaaat aattggttaa tattggtcca gttcagcagc agcaaccgat aatctctagc
     5581 gaaataacgt agtgacaata agtagcagcc gaataaagaa ccaaatagca agctagggat
     5641 aaaaaacaaa ggcagctgaa aagacagtcc gcttaataat gcgatcccac cagttaaaaa
     5701 actctgcaat atccatccga atctgaactt ttcttttaaa tattggttca tagagattgg
     5761 caaagaacgg ataaacagaa aattttcttg atccaaagaa ataagattgc tgataaaaga
     5821 agtctgatta actgttaaga aagccaatgc gatccctacc aggaaaacaa caccgattaa
     5881 cttattatct aaatcagtta attttatttc gcctcctagg gcaaaagcaa gaataaaaat
     5941 gattggagtt aacaaggaag aagaaagaac ttgcataatc aaattaggtt cttttactaa
     6001 ttgagcattg taactaaata aaagctgatg cacattttgg ttctttttat gggttctttt
     6061 tattttgaac tcactgtttg aatcagtcag ttgctctgta agctttggta ctatcagctg
     6121 ttttacagca aggaaaaatc ccagagccgt gatagctagt attccccaac ccaataaacc
     6181 ttgttgagaa agtggctgac tggcagcatc atagaatggc aataatatcg aaatcggatg
     6241 ccgatctgtt aactcatttt caatagtccc cgtttgatga ttcatccaca tgattccagc
     6301 aaatgcgatc agcatcgagc tgccaagcaa gatactagaa actgtttttc tatgtttttt
     6361 aaacactgct gttcgagcca aaccaaaaac aatcaaacta gccagacaaa aaagaataat
     6421 caaaaataga gaaaacaaaa ggattgagat aatgatagta agcaaaatcc ctagatcagc
     6481 tcgccatccg gttaaaataa acatgactag aagcggaaaa acaaaaggaa tgatggtcaa
     6541 agcaactacc aaaatctttg ctgaaaaaac ctgttcttgt ccaaaaggca gaggcaaaaa
     6601 tacttgcagg tcatttcctt caaaaaagat attataaatg actgatatac tttgagttaa
     6661 acctaacata ccaaaaagtg ccacgtaata agtaaagaaa cctggcaatt taacaaagtt
     6721 catagcaaac atagcaaatc cgtaaatcag taaaaaaatc agccctgaaa aaagattttg
     6781 agacaaaaga tagatagtta atcgctttcc cttttttcct ttctctcttg ctttattcgt
     6841 gagctgtgga ttaacatata aaagattaac tcgaattaat tctaataatt gtcgcttata
     6901 catacggtcc atctcctgtc gcttgccttc cagccatttt caaataaatc ttttctagcg
     6961 tttcatcagg tgagccattc aataattcac taactgcccc ctgataaatc aattcacctt
     7021 tcttcaaaat agccagttta tcgcataact gttgtgcggt atccaataca tgtgtagaaa
     7081 aaatgactgt ttttcctttt gcggcatgag ccttcatcat ttctttcaaa tcaaatgctg
     7141 cttgaggatc aagtccttgc aaaggttcat ccaataccca aatatctgga tctggcagca
     7201 acgtgccgat caaaatcgtt ttttgacgca ttccatgtga aaaacttgcg attgtttcat
     7261 tttgatgact atacatatca aaaagcgctg tcaattctgc taaacgcttt tctttttttt
     7321 gtgtgtctaa ttcataggca gctgaaatca agtcccaata ttctccagcc gtcaattgta
     7381 aaaaaatatc tggggaatca ggcacataac cgattttttg tttgatactc aatcgatttt
     7441 ccgtcaattt cattccatct actaaaatcg ttcccgatga tggttcaata atactaacta
     7501 aacttttaat cgtagttgat tttccggcac cattatgtcc taaaaatcca aaaatttccc
     7561 cttgtcttat cgagagagac aagtctttta atgcttgttt tttcccgtaa cttttactta
     7621 cattgatgaa ttcaatcatt tctgtactcc ttttgtttgt tattactttt tattgtaccc
     7681 ttcgttattg gtggagtaaa tgtcaaaaag agtgcataac aaaaaaggat agaaacgact
     7741 aggctgctac ttgggttgtt ctcttctaca ggaaaacact cttctaacag gtaagatatg
     7801 agtctttgtt ttctttaaaa tgattcattt tctatctttt cttgcataaa attctttagt
     7861 ttatcaatac ttctctttcc catctctagt tgttctccat ttgcaaatgt tactatttcg
     7921 tttactctgc taatagatgt aatgttttca gtattaatga tataagcttt taaatcttta
     7981 cataaatact catattcgag tgatttttta tattttttaa ttgtgccgtt tacgagaatc
     8041 tcttcatctt tcaatactag gcctatttta tttttataac ctggcatgct cattagataa
     8101 aggatttcat cgtaatggat atagtgatcg ctattaccat aacggacagt tgtccacctt
     8161 tttttcgtag catgttgaac aggatagttt tctttttctt tcaataagta actttggatg
     8221 ctttttttat ttttttcata agtgaattct tttatcaaat aaccactagg cgtaatcttt
     8281 cgattaataa cagttattgc tttattcgga tgagacgtta gataaacaat tttacacttt
     8341 tcattctttt ctctaagcat ttcgcctagt tcgataccgc tataataggc gttcaaatcg
     8401 atgtctagga aaaaaatatc tgaggaatag atttcattat ttttaatgaa ttcaacaaat
     8461 tgtataacgt tcaatccctg aacgatctca aatttccaag gacttaataa aattctggat
     8521 tcttccaaaa tttctactag cattttttca aaaaccaaac tatcatccaa aataaatata
     8581 cgcgttacca taaaaagtac ctccactcga tattttacta tcaaaatcat ttcatacaac
     8641 ttttcaacat acatgttagg aataaattgt aattctcttc acaatctttt ccaacacctt
     8701 tgaaaccgat atcaaatact ttcaggatgc tgcttctaaa ataaatatca aacaaatgaa
     8761 tacatcatgc aaaaaaagcc agacaaccga cgtccttgcg tgtcagctgt ctgagctatg
     8821 tttatttatt ttgttctttt aaaaagaacg atacacatac tgatttttcg gagcagagac
     8881 tttctcagcg tttgtaacaa ctacagatgg gatttcacgc ttgaatggtt gataatagtc
     8941 tgattggatg gttccttcta ttttatacca ttcatcattt ttaaccgtta ctccttccgg
     9001 catatgcgtc aacaaaccaa agactcctga atctgccaca caatggataa tgccgaatcg
     9061 gaacaagaag atatctgtat tgctatcgtt cggtgtttgg taaacaaatc cttcatagct
     9121 gatttttttg ccaatgaatt cgctagggta attataaatt agctccataa cttctaaata
     9181 gttttcatct gtgatcttga tagtttgctt atccccatac ttttctttca actcattcat
     9241 ttggtctaag tagtctgatt tgttgaaata aatactggta tccggcttta agtattgtgt
     9301 ctgcatctcc ggatccccta ctgattcttt acttactggg aaattaaatc ctttggcttc
     9361 tacgatcgta gtgtcgagac taactgttgg aaacaacgtc cccacgatta ctggcaaagc
     9421 tagcagtaca taagcgattc cacgttgata aggttttgac aacccatggt catgatcgtg
     9481 ctcgtgtgcc ttttcttctg cttttccgtt tttgacccac aaatacagct gaactaaagc
     9541 caaaagagca gataaaatca ttgaaagaat agctaagtag cgataatgga cattgatata
     9601 ttgatcgagt ttgccagaaa cttgcaagta catcatcaat tcgaaatacc cagataaaat
     9661 caaaaaccgc agcatatttt ccctcctaaa taaataatgt gaatccaata acgctaaggg
     9721 caatcagtcc gatcagcgat aaaataaagc gtcctgtaaa atatcgtttc atcatcagta
     9781 gatttttgat atcgaccatt gggccaaaga ccaaaaaggc agctacaggt gctgtgccga
     9841 acagacttaa caaagaagat cctataaacg catctgcttc tgagcaaaga gacatcgtaa
     9901 aggccaataa cagcatgatg ataatggcta aaatctttgt tgagccgagc gttagcataa
     9961 ttctagttgg taaataagtc tgcatcgcag ctgcgattaa tccgccaatg atcaaataac
    10021 gacctgtatc aaaaaattcg tcgattgaat gtgtcaacac atgcccagtt tgatggatca
    10081 gctgattttt tggttcattc ttctcacttt gttcttcttc atggacatga tgatggtgat
    10141 tttctgctac tttagacgtg attttttctt tcaatacagc gtctttttga aaataggcaa
    10201 gccagacacc aatcacaagg gcaacaaaaa agctgcctat cacccgccag cttgccatct
    10261 gccaagaatt gccaaatgcg ataaaagtgg aaaaaatcac gattggattg atgatcggag
    10321 cagtcagcat aaaagcaaac gctgtgtgaa ctggtacgcc ttttttaaca aattgatgaa
    10381 cgatcggtac gatgccgcat tcacatgaag gaaagaaaaa tcctaagatc gaaccagtaa
    10441 aaatagagaa gatacgattt tcaggaagcc atctcttcac acgttctggg gtcaaaaata
    10501 cttgtaaggc gcctgagata agacagccta ataatacgaa aggtaatgct tcaataacga
    10561 tcgataagaa aatcgtactc atctgcaaaa ccgaatttgg aataaaagaa aacatgagtt
    10621 tcctcctttt tatttctagt tcattcgctt tgcaaatata cgcttttatt caaaaaaaac
    10681 aagttgctct gtaaattttt acacaactct tattttttac caaagcactt caattgactt
    10741 tttctgttat tcccattaaa ttaaggtctc atacatatca cttctatcat aaaagggaga
    10801 ataaaaatga aacaaaccac taacttgact gaagttcaag acatactgca acagtcatcc
    10861 gttgccgtca tttatctttc gatgcctaat tgcagtgttt gtcacgctgt tcgtccacga
    10921 ctagaggaac ttctcacaca ttatgatatt ccggcacttc atttggatgc atttgaaaca
    10981 ccagaagtag ctagtaaatt tgaagtgctg actgcgccag ttgtcctgat ttttcatcaa
    11041 ggaaaagaag tctttcggca agcacggttc atcgacttta aaaaaatccg ttatctacta
    11101 gatgaactga cagcagaaga tgattccctt tcttatgaag aaatcttccg aacaaaataa
    11161 tcgttgtcca aacagaaaaa agcgattgaa caagtgaaag ttttgccttc atttgttcaa
    11221 tcgcttttta tttttattct gctgtatttt cggaatgcgc atttgcgtca gaatgcaaga
    11281 aatagatcaa ggtttgcaat tcattcgtca aatcaacatt ttggatcaat acatctgacg
    11341 gagcattcaa tcgagcagcg gtaaagttca agattccttt gattcctgct tccgttaact
    11401 gattgactac ttcttgtgct ctttcagcgg gtatcgttaa aattgctact tcgatttgtt
    11461 ggatcttgat ttgttctgac atatcttcca ttggataaac ggggataccg tctacgatgc
    11521 gcccaacgat ttcctcattc acatcaaaag cacaactgat acgtatgcta ttactttgat
    11581 ggaatttgta ttttagtaaa gcacttccca agttccctac accgatcagc gcaacatttg
    11641 tcaattgatc gtcgtttaat gtctttgcaa aaaagtgcat aagatcttct acatcataac
    11701 catacccacg ttttcctaat tcgccaaaat aagaaaaatc tcgacggatc gttgcactat
    11761 ctacttgaac ggcttcactt aattcagttg aagacacttt tttctttcca gcattattta
    11821 aaatccttaa atagcgataa tacaaaggca gtcttttagc tgtcgccttt ggaataatat
    11881 gatctttcac gaaatgacct ccacgtattg tctaattaca tctttgattt ctccatcata
    11941 gcagtattta taggaaaaaa tcaattccat tgctcagata ttgtgaaatt ttattcttta
    12001 ttcatttatt tatcatttct gcaaatctcg cagtataatg gaatttatgg tagactacta
    12061 attgaaaaat agttttttat ttgaggagaa ttcacaaagt agaaaagagg attccatgat
    12121 attattacaa gccaatcaga ttgctcgcca ttttggtgca gaagtcttat ttgaaaacat
    12181 acatttagaa atcagtacag gcgctcgtgt cgctttagtt ggcagaaatg gcgctggaaa
    12241 atcgacttta ttaaagatca ttgctggaat tgaagcacct gatgaaggaa agatcgctaa
    12301 aaataaaaca gcgactctcg gatacctcgc tcaggacaca ggacttgatt ccaatgaaac
    12361 agtttggaac gagatgctga aagcttttga acaagttaga caaatggaag agcggatgcg
    12421 cgagctagag attttcatta gcgaaggtac gccagacaca cctgcttata gttcttattt
    12481 aaaagaatac gaccggctgc aacacgattt ttcagatcga aacggctatg gttatgaaaa
    12541 tgaaattcgt tccgtattgc atgggtttaa attcgattct tcattttatg atcagaaaat
    12601 cgatacttta tcaggcggac agcgcacacg tttagcttta gctcgcatgc ttttgcaaca
    12661 ccctgacatt ttgattctag atgaaccaac gaaccattta gatatcgaga cactttcctg
    12721 gcttgagaat tatctgcaaa gctatactgg tgcattattg attgtttccc atgaccgtta
    12781 ttttttagat aaagtagtca atgaagtcta tgaattaagc agaagaaaaa tgcgccatta
    12841 caaaggtaac tattcgaaat atctagattt gaaagcagaa cagttagcaa gtgaatggaa
    12901 ggcttatgaa aagcagcaaa cagaaatcca taaattagaa gacttcgttg ctcggaacct
    12961 tgttcgcgcc tctaccacaa aacgagcgca aagccgcaga aagacattag aaaaaatgga
    13021 ccggctggat cgtcctcaag gggatgaaaa acaagcaaac tttctattca gtatagaaaa
    13081 gccttctgga aatgtagtct tgcaaatcga agatgccgct atcggatatg ataatgtcac
    13141 cttatctgaa ccaatcaatc tagatatccg tcgtcaagaa gcgattgctc tagtaggtcc
    13201 gaatggtatc gggaaatcga cgttgctgaa atcaatcatt caccagatcc cttttatccg
    13261 tggaaaagaa acgctaggga caaatgtcac catcggctat tacgatcaag gtcaagctga
    13321 tctgcataca aacaagacga tcctagcaga attgtgggat gagcatccta ccactccaga
    13381 aaaagagatc cgaagtgctt taggtgcttt cttattttct ggcgaagatg tagaaaaacc
    13441 aatccctctg ctaagtggcg gtgaaaaagc acgggttgct ttagccaagt tagcgatgaa
    13501 caaagaaaat tttttgattt tggatgaacc aacaaaccat ttagatattg acaacaaaga
    13561 agtattagaa aatgcattga tcgattatga cggcacactg ctttttgttt cccatgaccg
    13621 atatttcatc aatcggatcg cgacaaaagt cgtcgaactc tctgaaaatg gcagcaaagt
    13681 ctatctagga gactatgatt attatttaga gaaaaagcag gaagaagaag aaatcgccga
    13741 attgttggca caagatgagc cagaatctgc tcctgctccc acgaacaaat ctgattatta
    13801 ccaaagcaaa gaacagcaaa agcttatccg tagccttcaa cgaaaaatta ctcaggttga
    13861 agaagaaatg gcgcggatcg atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc
    13921 agaaaacctg gaagatcacg taaaactcaa tgagctgaac caagatttag aagctgctag
    13981 acaagatcaa gaaacaaaat tgacggaatg ggaagaacta agcttggaat tagaagaata
    14041 tgaaaattaa cg
//
LOCUS       DAYYGS010000069        13846 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000069 DAYYGS010000000
VERSION     DAYYGS010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.069"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..2270
                     /gene="acm"
                     /locus_tag="ACHA44_002061"
     CDS             105..2270
                     /gene="acm"
                     /locus_tag="ACHA44_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HFM8905323.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2542..3027)
                     /locus_tag="ACHA44_002062"
     CDS             complement(2542..3027)
                     /locus_tag="ACHA44_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HFM8905324.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3117..3462
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3507..4148
                     /locus_tag="ACHA44_002063"
     CDS             3507..4148
                     /locus_tag="ACHA44_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFM8905325.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4167..4802
                     /locus_tag="ACHA44_002064"
     CDS             4167..4802
                     /locus_tag="ACHA44_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFM8905326.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            4820..5635
                     /locus_tag="ACHA44_002065"
     CDS             4820..5635
                     /locus_tag="ACHA44_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8905327.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            5954..7390
                     /locus_tag="ACHA44_002066"
     CDS             5954..7390
                     /locus_tag="ACHA44_002066"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8905328.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7579..10026
                     /locus_tag="ACHA44_002067"
     CDS             7579..10026
                     /locus_tag="ACHA44_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8905329.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10256..11323
                     /gene="rlmN"
                     /locus_tag="ACHA44_002068"
     CDS             10256..11323
                     /gene="rlmN"
                     /locus_tag="ACHA44_002068"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8905330.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            11313..11519
                     /locus_tag="ACHA44_002069"
     CDS             11313..11519
                     /locus_tag="ACHA44_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905331.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(11516..12619)
                     /locus_tag="ACHA44_002070"
     CDS             complement(11516..12619)
                     /locus_tag="ACHA44_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8905332.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13172..13720
                     /gene="aac(6')"
                     /locus_tag="ACHA44_002071"
     CDS             13172..13720
                     /gene="aac(6')"
                     /locus_tag="ACHA44_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8905333.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         4741 a         2298 c         2939 g         3868 t
ORIGIN      
        1 ttagttaaaa gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga
       61 tatagtaggt tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa
      121 atattatttt atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg
      181 tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa
      241 cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa
      301 atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg
      361 aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg
      421 tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag
      481 aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata
      541 cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag
      601 cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata
      661 ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag
      721 ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta
      781 atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt
      841 tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa
      901 ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag
      961 aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata
     1021 aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg
     1081 gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga
     1141 ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac
     1201 agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag
     1261 taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa
     1321 gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta
     1381 tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca
     1441 ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag
     1501 ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata
     1561 tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa
     1621 acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa
     1681 atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc
     1741 aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc
     1801 aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat
     1861 acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta
     1921 cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta
     1981 taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa
     2041 ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac
     2101 aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa
     2161 gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga
     2221 tagtaactat ctgtgctggg atattcttac aaaaatataa aaaagtttag ttagaatata
     2281 atattttttg ttaaatatga ctgaaatcaa aagaataaaa ttccgattta taatcgaaaa
     2341 aagaagcatt tttatatatg cttctttttt cgtagaatca ataaatatgg atttggctaa
     2401 ctatggaaga atactcgtat tagttttcag cgttcatact agttgttaca ttgtttgatt
     2461 ggccaaaaga atttctgata tattttgtgc taaataaaga aaaagacgct tgaattggga
     2521 aataagaaaa ctactctatc attacaaatt ttcataaact ggcagatgtt ctaagtaaac
     2581 aagtatcttt tcaaagtcgg aaaaatccat aatcaacgta gtggtattga tatttggatg
     2641 aaagcctacg gtatcttgac gatcaatagc agcatcgatc acgacttgga tttttttctc
     2701 tttgtcgtgc atcaatgcta atggagtcac tgtacctgct ggaacgccta ggaggtcagg
     2761 aagctgttct tcagaaacaa aagataagcg cttttctttt aattgttcag ctagttgttt
     2821 taaatcggcg tgcttttcat caggcaagat gacaagatag atttgttttc cttttcttga
     2881 tttcaataat agatttttta cttgcggacc aggtagggaa aaagggacat ttttgactga
     2941 cgtaatcgca ggatgatcga ctttttgaaa agagataccc agttttttta gtagttcata
     3001 agcttcttgt tcggttgcac gactcatcgc gcatcaccct ccttttttta ttagtgtatc
     3061 ataaaaaaga gttgacaaat gaaaaataat catgtaaatt acacgtaaag ttaaaacaaa
     3121 tgacatcgaa aagaagagta gccgttaggt gtttttagag agtctctggt tggtggaaag
     3181 aggcagcagt taaccgtgaa acacatcttg gagtcgattt tctgaagcgt atagtaggaa
     3241 aatccgggga gcccgttaca gctgcggtcg ttgctagatg accagataag gctggctttt
     3301 ccgtgaggag tcggtaaaaa aaggtggaac cacgtgatta acgtcctttg atcttcggat
     3361 caaaggactt tttgttttca tctgaacggc aacgactgtg tgaggttttt tctgcaagga
     3421 aaaaataaac agaggtggaa ccacgtataa tacgtcctct tggcatttgc caagaggatt
     3481 tttttatttt aaaggaggaa aagaagatgg agtatttgat tgaagttatg ccgcaattat
     3541 tagtaggagc actcacaaca ttgaaattat ttttatttac cttgattggc tcattaccat
     3601 tagggattgt tttagcatta ggattgtctt tgcattttaa gccgctacgc tacttgcttc
     3661 aaacatatgt atgggttatg cggggaacac cgctgttgtt gcaactgatt tttatttttt
     3721 atgggttgcc gacgattggg atcgtttttg atcgtttcga agcagcactc attgccttta
     3781 tcttaaacta tgcagcttat tttgcagaga tctttcgtgg agggatacaa tcgattcctc
     3841 aaggtcaata tgaagcagcg caagttttgc aattaagtcc ctttcaaacg gtgacgaaaa
     3901 tcgtcatccc acaagtgaca aagattgttt taccttctgt agggaatgaa gttatcaact
     3961 tggtcaaaga ttcttccttg atctatatat taggtttagg tgacgtaatg cgtgctggga
     4021 aaatcgcgat ggaacgcgac gcgactttac tgcctttgct aggcgtggca atcatctatc
     4081 ttacattgac tggtattcta acaatcgtaa tgaaacaagt tgaaggaaaa ttaaattatt
     4141 accgctaact gataggagga agaaatatga ttgaattgaa gaaagtgaac aaagcatttg
     4201 gtgaaaaaaa aataatcaaa gatttgaatc taatcattcc agatgggaaa acattagcta
     4261 tcgtaggtcc ttccggtggc ggtaaaacca cgcttttacg tatcttggct ggcttagaaa
     4321 gttctgacag cggagaattt tatttagacg gtgtaccgtt caacccattc gagcttaaaa
     4381 tgaaagaaca agttgtagga gtcgtattcc aagacttcca gctatttcca catctgaata
     4441 tttttgaaaa cattacctta gcaccaaaaa tggtattgaa acagacaaaa gaagaataca
     4501 ccaaaaaagc agaagaatta gcggcttcat tagggattag tgatttattg aatatctatc
     4561 cttatcagct ttctggcgga caaaaacagc gggtagcgat tgccagagca ttggctatga
     4621 atccacgggt attggcttat gatgaaccaa caagtgcttt agatcctgaa ttacgccagc
     4681 aagtcgagcg tttgatccta tcattgaaag aagatggggt aacccaaatt attgtcaccc
     4741 atgatttagt atttgcagaa aatgtcggtg atcaattact aaaagtttcc ccaattcatt
     4801 aagaaaggag aagatagaga tgaaaaaaac cagttttcta ttcgctttga tcgcaggtct
     4861 tcttcttttt gcaggctgtt cgaacaaaaa aacttcagca gataattggg cgactatcaa
     4921 cgaagagaaa aaaatcgtta taggtttaga cgatacattt gttcctatgg gatttagaga
     4981 taaagacggg aagctcacag gatttgatat tgatttagcc aaagcagtct ttgaacaata
     5041 tgggattacg gtagactttc agcccattga ctggtcaatg aaggaatttg aactgaacaa
     5101 tggaacgatc gatttgatat ggaacggcta ttccaaaacg ccggcaagag aaaagaaagt
     5161 gcaatttaca aaaccttata tggaaaacga tcaagtgctg atcacaccta aagaatcagg
     5221 aatcacaaac tttggacaaa tgaataataa gagattaggt gcgcaaaatg ggtcatctgg
     5281 ttatgatgta tttacgaaac aaccagaagt attaaaagag atagtcaaaa accaagatgc
     5341 tgttttgtat gatagtttta atgaagcatt gatcgatctg aaaagcagac gggtcgatgg
     5401 tctgctgatg gataaagtat acgcagacta ttatttgaaa caaagaaacg aactagaaga
     5461 cttcaacata acaaaaggtt cttatgagag tgaggatttt gcagttggtg cacgcaagtc
     5521 agatagtaca ttagtgaaaa aaatcaatga agccctagat caactagaga aaacaggtga
     5581 attccaaaaa atatccgata aatggtttgg cgaagatgtt acgccgagaa attaacaaat
     5641 aggggactgt gacaaaagac gtgtcacagt ccctttttcc gaataaacag tgttctcctg
     5701 ccaactcctt gtgactcgga gctgaatggc aggctcacaa cctcttggta aacggtgttc
     5761 aaaagccagt ttttcggtat taagccaaat ttatgcaaaa atgtagaaag ccattttcac
     5821 aaaatttttc ttactactcg aaactaagcg actttttcac aacctccttt acatattttt
     5881 attttgttgt ggaaggggtt tcgtgttatt ctaaaagagg aacgattgaa ggttatagga
     5941 aaggaagaga tgaatggaac acaaagaact tacctcattt ccaaaagatt ttctgtgggg
     6001 ctctgcttct gcagcgtatc aagtagaagg agcttggcaa gaagatggga aaggagaatc
     6061 agtctgggat cgatttgtac ggatacctgg aaagacattt aaaggaacga acggtgattt
     6121 ggcagtagac cactatcatc gttataaaga agatattgct ttaatgaaag aacaaggtct
     6181 gaaagcttac cgcttctctg tagcttggac acggattttt cctaatggac gaggagaagt
     6241 caatcaagct gggctcgctt tttatgagag attgatcgac gaattgattg aaaacgatat
     6301 tgaacctatg ttgactttat atcattggga tcttccacaa gcattgcaag atgaatataa
     6361 tggttgggaa tcacgtcaga tcattgaaga tttcactcat tatgcagaaa cattatttga
     6421 ggcattccgc gggaaagtcc attactggat tagtttgaat gaacaaaata tcttcacttc
     6481 attaggttat ttgttagcag cacatccgcc aggtgtgaca gacccgaaac gaatgtatga
     6541 agttaaccat attgctaatt tagcaaatgc atctgtcatc aataaatttc acgagttaga
     6601 aatgcctgga aaaatcggac caagctttgc gtatacgcca aattatccaa ttgacagtaa
     6661 tcctgaaaat gttctggctg ctgaaaatgc agaagacctg atggccaatt attggatgga
     6721 tgtctatatg tggggaaaat atccgattgc tgccatgagg ttcttagaag aaaaaggatg
     6781 ggcaccaacg attgaagctg gcgatgcaga attgctagaa tctgctaaac cagattttct
     6841 tgggatcaat tactatcaaa cggcgacgaa tgcattcaat ccattaaacg gagttggtgc
     6901 tggaaaaatg aatacgacag gaaaaaaagg ttcatcagaa gaaactggag ttcctggaat
     6961 gtataaaaaa gttgagaatc catttgtcga acgaacaaat tgggactggg agattgaccc
     7021 agaaggatta aggatcggac tgcgtcgtat cacaagccgt taccgcatgc cggtactgat
     7081 tacggaaaat ggcttaggag agtatgacaa gttgacagaa gataaacaaa tccatgacga
     7141 ctatcgaatc aattacttac aaagccacgt caaagccatc aaagaagcaa tttcggatgg
     7201 agcagaagtc ctaggctatt gcacctggtc ttatacagat ttgcttagct ggctgaacgg
     7261 ttatcaaaag cgatatggat ttgtatatgt tgatcaagac gaaacacaag aaggatcttt
     7321 ggaacgatat aagaaagaca gttattactg gtaccaaaaa ctgattgaag aaaatggaga
     7381 gaacctttag ttcacatact taaaacaaga aggtacggct ggatatggcc gtgccttttc
     7441 ttgataaaat catttagaaa ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag
     7501 cgtcagttct ttcaaacaaa actttagttc ccttactttt ttttgtataa tgaaacttga
     7561 tgaaatgagg gataataaat ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg
     7621 aaaaaaacga aaaagcaaaa acagcagcag gaacaaatgc tgacgattgg tgttgggttt
     7681 gccttgattt tatttgctgt cttcggcttt ttaaaattag gttttttagg aatattgatt
     7741 gcaaacggct ttcggatcat tgcaggaaat acttaccaaa tcctctgtct tttattagcc
     7801 gtattaggat tttggattgt cattaagaat acagagtttt caattggtaa aaaccgacgc
     7861 tggtttggcg gcatactctt ttatttcggt atcttattat tgttacatgc ccatttattt
     7921 ggcaaactcc atactgggga accaaatatc atggggacga catgggattt gttagccagt
     7981 gatataaaac aaagtcaagt agacaataac gttggtggtg ggatgattgg agcaatcctt
     8041 tatcatttta cttacttctt gattgcacag cctggaagtt atttagcagc agtgctatta
     8101 ctggcaggag gagcattcct gttcagtaat ctggaaggct atcagctgtt aaatggtatc
     8161 caatcaattg gagaacgagt acaggaatta ttggaaggtg atcctgccaa gcaagctcga
     8221 aagcaagcgg caaaggaaga gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc
     8281 ttagctgcaa aagaagctgc agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg
     8341 agccagcaaa gggaaaatac tgctgatgaa tggcaggaac cagaaaatac agagccggag
     8401 cagctaagtt ttgttccaat cgacagtttt caagaaaata tccatcctgc gaatctagag
     8461 aaaccagtac cagatacacc aaaacagacc aatacagcag aaggtttttc agatgagttg
     8521 ccagaggatg acggtacctc attagaattt gagattgaag cagaacagga aaatcaagac
     8581 tatgaattac cgacagttga tttgcttgat tctataccaa ctgtggatca aagtgatgag
     8641 tataaaaaaa ttgaaaaaaa tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc
     8701 gatgccaagg tcgtcaaagc cagcttagga ccatccgtca caaaattcga agtccagccg
     8761 gctgtagggg taaaagtaag taaaattgtg aacttaacag atgatatcgc gcttgcttta
     8821 gcggcgaaag atgtccggat ggaagcaccg attccaggta aatcactgat tggtattgaa
     8881 gtaccaaatg gaaagatcag tatggtttcc tttagagaaa tcatcgaagc acaaccaaac
     8941 cacccagata aattactaga agtaccttta ggacgtgatg tttctggacg ggtacaaacg
     9001 gctgatcttt cgaagatgcc tcacttactc gtggctggtt cgactggtag tgggaagtca
     9061 gtagcaatta atgggattat cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa
     9121 ctgatgatga tcgatccgaa aatggttgaa ttgaatatgt acaacggcat acctcatctg
     9181 ttgacgccgg tcgtgacgaa ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa
     9241 gaaatggaag aacgatatga aaaattcgcg gcaaccggag taagaaatat ttctggttac
     9301 aatgaatttg ttcaacagaa aaacttggaa aacggaacga aacatccaac cttaccattc
     9361 atcgtagtga tcgtagatga attagctgat ttgatgatgg tagccagcaa tgaagttgaa
     9421 gatgcgatta ttcgtttagc gcaaatggct cgggcagcgg ggattcatat gatcctagct
     9481 actcaacgtc caagtgtgga tgttatcaca ggaatcatca aagcaaatgt accttccaga
     9541 atggcatttg cggtatcaag cggtacagat tctcggacga tcattgattc taatggggca
     9601 gaaaagctct tagggcgagg agatatgcta ttcttgccaa tgggtgagaa taaaccgatc
     9661 cgtgtgcaag gagcattcat ttctgaccac gaagtagaac gggtcgttca atttgttaca
     9721 gatcagcaag aagctcatta tgaagagaaa atgatgccga cagatgaagt tgaaacgaca
     9781 ggtgctccag aacagccaca agatgagctg tttgaagaag caaaagcact tgtcgttgaa
     9841 atgcaaacgg caagtatttc ccttctccaa agaagatttc ggatcggtta caaccgagca
     9901 gcacgtttag tagatgaact ggaagctcat ggagttgttg gcccgtcaga aggaagcaag
     9961 cctcggaaag tattcattga gcaggaagaa acatcagaaa cgttgccgga cgataatgac
    10021 atgtaaaact tgtgtgatga agggttgaaa aacgaataac caataaaaag acaaggcatg
    10081 atgataccaa aagaaaaaat ggtttttttc catcttttct aatacggtat gatgtcttgt
    10141 ctttttagtt tttgtgagat taaagttcta ttcgatttat ttgtgaagta ttgcattaag
    10201 gaaatgaatc atgtatagta tgtagcgaaa ctttgagaaa aagagggaaa atactttgga
    10261 aaaacagtcc atttatggct tgacgaatga agaattaatc aattggttta tagaaaacgg
    10321 cgagaaaaaa ttccgagctg cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa
    10381 tttcacagaa atgacgaatc tatcgaaaca attgatcgaa aaattatcgg agcattttat
    10441 catcaatcct ctaaaacaga tggtcgtcca agaagcttcg gatggtacag taaaatattt
    10501 gtttgaactc cctgataaaa acatgattga gactgtactg atgagacaag aatatggctt
    10561 gtcagtttgt gtgacgacac aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg
    10621 gctgctcaaa aagaaccgtg atctgactgc tggagaaatt gttgcacaga tcatgatggt
    10681 ccaacattat tttgatgagc gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg
    10741 gatcggtgag ccatttgata attacgacaa tgtaatggac tttttgcata tcatcaatga
    10801 tgccaaagga ttggcaattg gtgctcggca cattacagtt tccactagtg gattagcaca
    10861 caaaatcaag gaattcgcta ataacggctt gcaagtcaat ctggctattt ccttgcacgc
    10921 gccgaataat gaggtgcgaa catcgatcat gcggatcaac cgaagtttcc caattgagaa
    10981 attgatggaa gctgtggatg agtatttaga gaaaacaaac cgtcgaatca cgtttgaata
    11041 tatcatgttg aatcaagtaa atgatagacc agagcatgcg caacaattag cagatctatt
    11101 aaaagataag aaaaaattag catatgtcaa cttgattcca tataatccag tcagtgaaca
    11161 tgatcaatat tctcgcagtc caaaagctga tgtattgaga ttttatgatg tattgaagaa
    11221 aaacggcgta aactgtgtga tacgtaaaga acatggtaca gatattgatg cagcttgcgg
    11281 acaattgcga agcaagcaaa tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt
    11341 tttcaattac cataaaagga gcagttttct taattaagga actgttcaac ttaaataaga
    11401 gaaaagattc tatagcgaaa gctataaagc tttttttgac aagaaaaacc ctcatcaagc
    11461 aaagaaatgt cctcaatttg atgacagcct gcttgatggg ggttttagca attatttaac
    11521 tgattttctt atatccttgc tggctttctt gtgtagcatg ataagtttct acgtcaagaa
    11581 tggcctgttt aaatttttct cgggtgattt cataaggcac atgtttaata tcatttgttg
    11641 ccatggcttt atctaagatt gcttctaaat cggtcgtgac attgagattc atgtcggtta
    11701 acgatactgg aagttggttc tttttcataa acacatagat tttatcaaac aaatcgaact
    11761 gctgatccat catcaataag gtcagtacac cataactgac agatgctccg tgaaggtatc
    11821 cctccatttt ttctagaaca gtcgtaccgt aatagaatga gtgtgccata gcagaattat
    11881 agttattgtt aactaaaaca gaaaccaatc cggtagtaac aatgatatcc gaaacgactt
    11941 gttcaattgc atatgaggga cgatcgtcgg cactatcttt caatgcttgc gtaccataag
    12001 tcaagagacg atcaatgcac atttttccga tttggattcc taatctatcc gtgtagccta
    12061 gatcatcgcc acgagcagaa aaagtagatt cgacttcttt ggataaagca tcgccaatac
    12121 ctgcccataa gtatttatca ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta
    12181 caggtggttt tgaaggaaag aagagtccgt ccattacgcc ttcttcgtta tagatgacac
    12241 ataccgcagt gacgggcgaa cagttcgaac caatagtggg aaaggtgaag actggtttag
    12301 ctagtttttc accaatgact tttacagtat cgcaagcacg gccaccaccg actgcaaaca
    12361 ggacgtctgc tttttggaca ctttcatgct ggatcaactg ttcaatattt ccataagttg
    12421 aattaccccc gtaccagaga ctggcgacaa cctctatttc ctgtgtcaaa gctactttta
    12481 ttttgttttc tgcctttgaa agtgcagtct tccctccaat cagcactact tttgtcccat
    12541 attctttgac gattccattt gctttttcat acgcatctgt tccaatggta taatttggta
    12601 agaaaacact ttgactcata ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt
    12661 aaagagtgat cgatctgact gcggctgtag atggagtaga ggtgaaccta agacaggata
    12721 agtgatgaat aaggtggcat gaacaccttg aaatcatcaa taagtatcct aaaaacgaac
    12781 aagtacaaaa acattgaaaa cgacaataat gaatttttgt tcctcttagt atttctaccg
    12841 attccctttg cttttttgta attatctaaa aattctaaaa attaagatta ttatatgaaa
    12901 acaggtttta tttgtcaatt cataattggt tttttttaaa gcaaaagata atctatatca
    12961 tgatgaacaa acagagtttt ttcctccaat tgatggcttt cctgatcttt cctattttga
    13021 ataaagtgtt tctgtataat ttttttattt tataagtaag ataaataatg aaagaataga
    13081 gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc aaaaggactg
    13141 ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg accgtaataa
    13201 tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg aagaatatgg
    13261 agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg cggtagcagc
    13321 ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg gtatcacagg
    13381 ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa taggtactcg
    13441 attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga tttatttagg
    13501 tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg agcatacatt
    13561 tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct atgaaaaatt
    13621 aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac cggatatttg
    13681 gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga
    13741 agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga atttttgtga
    13801 aattccaact tatttccgaa gaagctactt ttggaacacc gtgtac
//
LOCUS       DAYYGS010000070        13705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000070 DAYYGS010000000
VERSION     DAYYGS010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.070"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..902)
                     /locus_tag="ACHA44_002072"
     CDS             complement(<1..902)
                     /locus_tag="ACHA44_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HFM8905334.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="ACHA44_002073"
     CDS             1185..2153
                     /locus_tag="ACHA44_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8905335.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="ACHA44_002074"
     CDS             2266..2544
                     /locus_tag="ACHA44_002074"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905336.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="ACHA44_002075"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="ACHA44_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFM8905337.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="ACHA44_002076"
     CDS             3001..3756
                     /locus_tag="ACHA44_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8905338.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="ACHA44_002077"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="ACHA44_002077"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFM8905339.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="ACHA44_002078"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="ACHA44_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFM8905340.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="ACHA44_002079"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="ACHA44_002079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFM8905341.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="ACHA44_002080"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="ACHA44_002080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HFM8905342.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="ACHA44_002081"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="ACHA44_002081"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFM8905343.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="ACHA44_002082"
     CDS             8493..9035
                     /locus_tag="ACHA44_002082"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFM8905344.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /gene="atpA"
                     /locus_tag="ACHA44_002083"
     CDS             9058..10614
                     /gene="atpA"
                     /locus_tag="ACHA44_002083"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFM8905345.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="ACHA44_002084"
     CDS             10628..11530
                     /locus_tag="ACHA44_002084"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFM8905346.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="ACHA44_002085"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="ACHA44_002085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFM8905347.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="ACHA44_002086"
     CDS             12973..13395
                     /locus_tag="ACHA44_002086"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFM8905348.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3735 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag acttt
//
LOCUS       DAYYGS010000071        13530 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000071 DAYYGS010000000
VERSION     DAYYGS010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.071"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..887
                     /locus_tag="ACHA44_002087"
     CDS             <1..887
                     /locus_tag="ACHA44_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HFM8905349.1"
                     /translation="LQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGVSS
                     AITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIGHY
                     TNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTEDT
                     LEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYPDN
                     LNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCTLF
                     GKGFGNYDQDIKLKVYYS"
     gene            complement(960..1784)
                     /gene="nadE"
                     /locus_tag="ACHA44_002088"
     CDS             complement(960..1784)
                     /gene="nadE"
                     /locus_tag="ACHA44_002088"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFM8905350.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1796..3268)
                     /locus_tag="ACHA44_002089"
     CDS             complement(1796..3268)
                     /locus_tag="ACHA44_002089"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFM8905351.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(3905..4267)
                     /locus_tag="ACHA44_002090"
     CDS             complement(3905..4267)
                     /locus_tag="ACHA44_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HFM8905352.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            4490..5242
                     /locus_tag="ACHA44_002091"
     CDS             4490..5242
                     /locus_tag="ACHA44_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HFM8905353.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(5382..6305)
                     /locus_tag="ACHA44_002092"
     CDS             complement(5382..6305)
                     /locus_tag="ACHA44_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905354.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(6320..6592)
                     /locus_tag="ACHA44_002093"
     CDS             complement(6320..6592)
                     /locus_tag="ACHA44_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905355.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
     gene            complement(6936..8123)
                     /locus_tag="ACHA44_002094"
     CDS             complement(6936..8123)
                     /locus_tag="ACHA44_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HFM8905356.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            complement(8536..10161)
                     /gene="groL"
                     /locus_tag="ACHA44_002095"
     CDS             complement(8536..10161)
                     /gene="groL"
                     /locus_tag="ACHA44_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFM8905357.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(10212..10496)
                     /gene="groES"
                     /locus_tag="ACHA44_002096"
     CDS             complement(10212..10496)
                     /gene="groES"
                     /locus_tag="ACHA44_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFM8905358.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10721..11383
                     /locus_tag="ACHA44_002097"
     CDS             10721..11383
                     /locus_tag="ACHA44_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8905359.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            complement(11459..12751)
                     /locus_tag="ACHA44_002098"
     CDS             complement(11459..12751)
                     /locus_tag="ACHA44_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8905360.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            12921..>13530
                     /locus_tag="ACHA44_002099"
     CDS             12921..>13530
                     /locus_tag="ACHA44_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFM8905361.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQH"
BASE COUNT         4033 a         2794 c         2351 g         4352 t
ORIGIN      
        1 aactacaaat aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga
       61 tcaagatcat ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac
      121 ttggagtgag cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca
      181 tcaaaacaga aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata
      241 tcagttttcc agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg
      301 gtcactatac aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta
      361 tcgggaaagt ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc
      421 tctggtttac cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata
      481 cattggagat gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca
      541 attggccttc agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc
      601 ctggagattt tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta
      661 atcaatacgg cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag
      721 aacccttgtc aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc
      781 gaatcgaagt gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat
      841 tcggtaatta cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc
      901 tattttataa aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt
      961 tatttccaga aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc
     1021 caccatgctt cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca
     1081 tcaatttcat tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct
     1141 tcaagatctg cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc
     1201 aattgtttcc cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca
     1261 ccaaatttcg tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct
     1321 cctgctcttt ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt
     1381 cctttgttaa aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca
     1441 acagctggtt tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct
     1501 tttgcatctt cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag
     1561 ttttcatctt ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct
     1621 aaagtagaat cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt
     1681 ttcaaatatg ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt
     1741 ggtttgacac ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg
     1801 cttctgtatg aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt
     1861 tccagcattc agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact
     1921 cttcccatag tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta
     1981 attcgtaaac tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt
     2041 ctcgaacagt tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt
     2101 ctcgagggtc ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc
     2161 gagtgatgcg ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata
     2221 acttgatcgt atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg
     2281 caccaagggc tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa
     2341 ttttagcttt ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat
     2401 agatttttgc ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat
     2461 aggccatatc tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt
     2521 tcgccacgcg aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga
     2581 acacgcagtc tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag
     2641 attggactaa cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac
     2701 gtacattgct tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg
     2761 catccagttc ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag
     2821 atttgatacg agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg
     2881 ctgtttctac taattggcag tgcgctaaag gcccttccac ttggattaat ggttcatttg
     2941 caaaaactag ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta
     3001 aatactccaa aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat
     3061 cattgaacgt cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt
     3121 atccgtggtt aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc
     3181 gacctaattc ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca
     3241 aactgtcgtc tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt
     3301 cctacccatt ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa
     3361 gcacatgttt tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt
     3421 cttattggta tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca
     3481 cgaagaaatt agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac
     3541 aatcgtagcc gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata
     3601 atcaatggca aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca
     3661 aagaaacgga tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga
     3721 tataccttgc gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa
     3781 gtaagaaagc agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc
     3841 cttgtttttt attgtataca ttagatatgg agcaggttac cataaagatg attattagtc
     3901 gttattaaat agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac
     3961 gtttgaattg tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat
     4021 cttctcgtgt catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt
     4081 ttttcttcgt attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt
     4141 gtgttcgatc taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt
     4201 ccactacttt aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta
     4261 gcttcatctc attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac
     4321 catctttcct agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac
     4381 cttttatcac ttaatagtat aacataattt cgtgagaaaa aaatacaatt ttttcttgac
     4441 catctcgtta cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac
     4501 aataaagaaa atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga
     4561 gattggactg ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa
     4621 agaagtcgat cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga
     4681 tatccaagag gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca
     4741 gcaaatgttg ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac
     4801 acttgatatg tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa
     4861 acaacaaaag ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg
     4921 aaaaaaaaag gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga
     4981 agaaatgcaa actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc
     5041 agaagatgaa agcttagcag accgtatctt ccaagctcat aagaagtggt tctcttacag
     5101 ctggcctgaa tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga
     5161 acggttcact gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga
     5221 aattatccaa aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg
     5281 acctaataac aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc
     5341 tccattcttt cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta
     5401 taaggtattt tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg
     5461 caagcttcca gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta
     5521 tcgcgtccaa tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca
     5581 accatatccg aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct
     5641 tgttttcctt tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga
     5701 aaaagttgat gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc
     5761 cccaacgtga tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta
     5821 attgattgtt ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa
     5881 aaaactagag gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac
     5941 gtaatgatca atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt
     6001 tctttatgat agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca
     6061 atgatcccac tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca
     6121 atgcttttcg tttttccata acttaactgt ctggcatttg cattagggat ataatcgact
     6181 tctttcatga ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg
     6241 attacacgtg aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt
     6301 gccactgctg cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg
     6361 gaccgcattc aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc
     6421 cccaatatat atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat
     6481 tcctattcaa cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg
     6541 cataaaaata attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc
     6601 ctccttttga tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt
     6661 gccaaagaaa agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat
     6721 acattataat gatactgtaa atatatcttt attattagtg aatggagtca agtccacaat
     6781 cttgtgtaaa agttcaacct catcaaaagc acaatgtttt gcgttgtgaa cctaactgcc
     6841 atgcctttct ttctgaggag agaggtcagg agcagtagcg cagcgactct tgatgtctct
     6901 cctcagaaag aagagactcg cacaggttca tggtgctaaa tgttattcga attaaattct
     6961 aaatattttc gtggagaagc cagccactca gtgtcttttt ccatcaaaag tgttccaatt
     7021 atccgagtga tggaattgat atttggaaaa atccgaatca ctcgttctct ccggcgaatc
     7081 tcttcgttca accgttccaa cacattggtt gtacgaatcc ttcggcggat attttcagga
     7141 aaagacaaaa tttgaatagc atcttcaaag ccgttttcta aaatgtcgca agcagcactt
     7201 aattttgaat cacaaccata tttttccaag aagtgctctt ttctttcacg tgtcaattca
     7261 agttcagagg ctttaaagat actcttcaac tcgtctttta catcagaaga aactttcttt
     7321 ggcagcttat caaaaatatt tctcaaaaag tgggcttggc atctttgcca gctcgcacct
     7381 aaaaagttct tgcgaacagc cttaactaac cctttgtgtg catcagaaat gaccagtttt
     7441 acaccgaaca aacctcgttg aacgaacgat tgaaaaacgg tatcccaatt atcttccgat
     7501 tcgccgtctt gaacatcaaa accaagaatc gtacgtcgtc catcagaatc aatgccgata
     7561 ccaatatata caccttttga gacaatacga tgattttccc ttactttcat atacagggca
     7621 tcacacatga aaaatggata ttccgagcct tctaggctcc ggtttctcca ctcaaaaaca
     7681 aatggatcca actgtttcat cagatttgaa acgaatgatt tagaaacagt cgcaccatcc
     7741 gttagcagtt caactgtttt agttactttg cgagtggaga caccagaaat gaccatttca
     7801 atcatggcag cgatcaaagc tttttcgctt cgctgatatc tttcaaagat agtaggacta
     7861 aacttaccgt ctctggttcg tggaacatga agttctaacg taccaattcg agttgtaaac
     7921 gaacgatcgt agtaaccatt tctttgtgat tgccgttctt ctgaacgttc ataggcattt
     7981 gcctgaatat attgatttcg ttgttcttcc atcagctgat taaacatgat agtcataagt
     8041 gtttttgcgg tatcattcgc accgctttcg tttataattg cttgaatttc ttcgctttca
     8101 aaagtaaaat gtacttgagt cattgatatc agtcctattc gagtatgttt tctgcaaaaa
     8161 acattgtact cctaactgag atcaatggct tttttgatgc ttcactttta cacaattata
     8221 tggacgtaat cgtgaatgga tatttacttt taatagataa aatattataa ctattaaaaa
     8281 taactatttt cattataatt acacacaaaa aagagcgtgg gacaaaagta aagaacactt
     8341 ttgtctcacg ctctaaacac gtataaacag cggaagcaga agcaactcct tcggaaataa
     8401 gctgaaattc acaaaaattt gaaaagcaat ttccgtgaat ttcttcttat ttcttggagt
     8461 taaacgcttc tgttccagcc tctttttatc tgtctttcac taatgatcag ttgtagataa
     8521 gcaagttgct ttttcttaca tcataccgcc catcattgat ggatccattg caggagctgc
     8581 tggcgctgct ggttctggtt tatctgcaac gactgcttct gtcgttaata acaaggcaga
     8641 aacggaagcg gcattttgca aggcagaacg tgttacttta gttgggtcta cgatacctgc
     8701 ttcaaccatg tttacccatt ctccagtagc agcattgaaa ccagtgccta gttccacgtt
     8761 cttcaattta tcaacaatca ctgatccttc ataaccagcg ttttcagcga tttgacgaat
     8821 tggttcttct aaagcacgaa caacgatctt gatacctgtt gccacgtctc cttctgcttc
     8881 tacagcagaa actttgctga taacgttcac tagagctgta ccaccacctg agaccattcc
     8941 ttcttctact gcggcacgtg tagcattcaa tgcatcttca atgcgtaatt ttaattcttt
     9001 caattctgtt tcagtagctg caccaacttt aacaacagct acaccacctg ctaatttagc
     9061 taagcgttct tgtaattttt cgcggtcaaa atcagaagtt gtttcagcaa tttggttttt
     9121 gatcaattgc acacgagctt cgattgcttc tttttctcca gaaccttcta cgatcgttgt
     9181 attgtcttta tctacaacaa ctttagaagc atttcctaag ttttcgattg ttacatcttt
     9241 caattctaaa cctagatcgt cagtaattac tgtaccacct gtcaaaatag cgatatcttc
     9301 aagcattgct ttacgacgat cgccgaaacc aggagctttt acagctacta cgttgaatgt
     9361 tccacggatt ttgttcaata caagagttgg caatgcttca ccatctacat cgtcagcaat
     9421 aatcaacaat ggacgtgatt gttgcaagat ttgttctaac aatggcagaa tatcttggat
     9481 gttagagatt tttttgtctg tgatcaagat atatgggttt tctagaacag cttccatttt
     9541 gtcgttatct gttaccatat attgtgataa gtaaccacga tcaaactgca taccttctac
     9601 aacatctagt tctgtttcga tcccttttga ttcttcgatt gtgatgacac cgtcgtttcc
     9661 aactttttcc attgcatcag caatcaaatg tccaacttta tcagaacccg aagaaacagc
     9721 tgctacttgt gcaatggctt cttttgaatc aacaacagtt gagatattat gcaattcttc
     9781 gactgctgct tttgttgcca attcgatccc gcgacgaatg cctagaggat tagcacctgc
     9841 agttacgttt tttaaacctt cgcgaacgat tgcttgtgtc agaacagttg ctgtcgttgt
     9901 accgtctcct gcaatatcat ttgtttttga tgctacttct gatacaagtt ttgcacccat
     9961 gttttcaaaa tgatcttcta attcgatttc ttttgcgatc gtcacaccat catttgtgat
    10021 caatggagag ccgtaagatt tttctagtac gacatttcgg cctttaggtc ctaatgttac
    10081 tttcactgta tctgctaatt tatctacccc gcgtaacata gctgcgcgtg cgtcttctgc
    10141 aaatttcaat tcttttgcca tgattgattc acctcattga ttatttttga ttattttttt
    10201 atcttccaaa tttactcaac aatcgccatg atatcttttc ctgcgacgat cagatattct
    10261 tttccttcgt attttacttc tgtaccggcg tatttttcga acataacttg gtcgcctgct
    10321 tttacagcgg caggaacttt ttcaccgttt tctaacaaac ggccttcacc gactgcaaca
    10381 actgtcccag tttggggttt ctctttagca gcagaagcta aaacgattcc tccgacagtt
    10441 ttttcttctt cttttgcgac ttcgataatg acgcgatcac ctaatggttt taacacgata
    10501 aatccctccg ataactcttt ttttaatctg ttagcactct taaccttcga gtgctaacct
    10561 acatttttaa taatactcat tctccctctt ttttgcaagc ttttaaatca aagaaaagaa
    10621 attttattct gacagctgct tattgaaatt atgatataat tgaaatcaat cgatataagc
    10681 ttgccttgca tgcttgttat cccttaaaag gaggaattac atgtctttaa aaaaatatag
    10741 cttcctcagc attttttgct atggtctaat ttttatctca cccttgatcc tagcttcgat
    10801 aggacttgta aaatctacgt ctgaattgat cacagcaact gcagtcatgt atatactagg
    10861 cgccgttgtc ctagctattt tttattttaa gcaacgtgaa cccttagcga tcgaatcagc
    10921 ggtaaaaaaa gcttctgctc caaagattgt tatttatgga ttggttggaa tctttgtcgc
    10981 attgattctg caaagcatcg ctgtcatgtt agaatctgta ttgtttggca aagcaacccc
    11041 ttccgagaat acacaaaata tcatccagat gatcatggaa gcacccgctt ttattttggc
    11101 gacaactgtt gctggaccca tcatggaaga gttcgtattc cgccgcagta tactggggat
    11161 catcagtcga tattcaaatt tttgggtcgg agcagtgatc agctctttgc tgtttgcgtt
    11221 tgctcataat gatggtcatc tgctaatcta ctttttccta ggtttcttct tcagcctaga
    11281 gtacaaagca actggtcgga tctggacatc gatgatcaca cacgtaggaa tgaacacatt
    11341 agttgttctt gttcaattag ctgtccaaaa agggctagta taaacatgac tataaatact
    11401 cctgtcatcc tagattggtg attttcccaa tctaaagcga caggagtatt tttgtttttt
    11461 agtcgttcat cttttctgct gctccatcat tttttgtctg tctttccttt ttgcagtttg
    11521 cagaatctat ttttttcaaa aatgggaaat aaatgaaaaa actaattgac aacacgatac
    11581 cttgtaaaat cgctgcaggc actccgccta ctaagaaacc cgagaggatc ggcggtgttg
    11641 tccaagggac ttggactgct gtaaataaag gaacgaatcc aaaatataaa gcggaatagg
    11701 taatcaatcc tgaaacgacc ggtgcgaaaa taaaaggaac agccatcatc gggttcatca
    11761 cgatcggtgt ggaaaaaata attgattcat tgatgttgaa aaaggctggt aaaagagcaa
    11821 gatttcctaa ttctctgtat ttcttcgatt tcgcaaaaac cgtcataaaa aatacagccc
    11881 ctaacgtcaa tccagcaccc gtaaccgtca tgaactgatc gagaaattgc tgagtaacga
    11941 tatgtccgcc atttgccacc gtcaactctt gtcctgaggc taagatttct gcattcgcta
    12001 atgaattcgc ttgcaagacg ggactaacca acccattgac catcgcagat ccatgaagac
    12061 caaataacca taaaaacgga acaaaaaaac ctaataaaag cacaccaaac aacgtgtcta
    12121 tgaacgattg aatgggaagc tggacatatg tccatactcc ttgaacaaca gtcattctga
    12181 acaactgttg aaaaaccgct gaaccgataa gcgtaaaagt gactataaca atgattggaa
    12241 tcaatgaagt aaaagactgc caaacatttt caggtatctt ctctctttgc ttccaccctt
    12301 tcgcaagtct tactaaacgt gcatagcccc atccgatgat gagcccagtt gtaactgctc
    12361 ctagtatccc ttcagcacct gtccagacag tatctatctg agtatttagt ggctgaataa
    12421 gcagtagata agcagacaag gctgttatgc cgactggtaa gcgatcgatg tcttcttctt
    12481 ttgcataagc gtatgcaatg gcaaaaacga tccaaaaggc tatcacatca aatgtagcct
    12541 ggtatacttg tgtaaaatac atagaaaatc ctgttttgtc tatccactga gcgatgtaat
    12601 ccacaggaaa atgagcaaat aagaaaaaca gcgagccaac aataattatt gggatcgtgt
    12661 aagtcattcc attacgcaaa gcagttaagg gtctaagatt cacaacagat gttatcattg
    12721 gtaaaaaggc acgatctaac cactctttca tttcttatct ccttcaactt ctatttttat
    12781 caatcagttt tcttaattta actataccca atgatcacga accatgaaag gaatgcgcac
    12841 tgagcttttc aaaaatcgga ttctttgacg aatattcagc aactgcttac aatggacagg
    12901 aataaaaaag gaggtttttt atgaaaattg cactcattgg agctactggc catgcaggtt
    12961 catttatttt agacgaagca ttgtccagag atttagacgt gacggcaatc gtgcgtcatc
    13021 cagaaaaatt gccatcagat gttccgttta tacaaaagga tttatttgag ttgacaacta
    13081 ctgatcttgc atcttttgat gttgtcgttg atgctttcaa cgctcccaaa ggaaaagaag
    13141 aaatgcatca aacttctttg tcccatttga ttgagatgct tgaaggaacc gatacacgct
    13201 taattgtcgt tgggggtgcc tcttcgttat tcttagatac agaaaagacg actcgtatga
    13261 ttgaccaagt acctaaaaat gcacccttct atccaacagc tttcaatatg tacgaaggat
    13321 tgttaaaatt gaaacaatct aaaaatctac attggactta tatcagtcca gcttctttat
    13381 ttgatccaca tggcaaacgt acaggtactt atttgttaag tgatgattat ttacaaagaa
    13441 acgctgctgg cgacagtacg atcagcatgg cagattacgc gattgcatta gtagatgaga
    13501 tactggatga gaaacatgaa aagcagcaca
//
LOCUS       DAYYGS010000072        13516 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000072 DAYYGS010000000
VERSION     DAYYGS010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.072"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(334..894)
                     /locus_tag="ACHA44_002100"
     CDS             complement(334..894)
                     /locus_tag="ACHA44_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8905362.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="ACHA44_002101"
     CDS             1036..1650
                     /locus_tag="ACHA44_002101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905363.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="ACHA44_002102"
     CDS             1839..3719
                     /locus_tag="ACHA44_002102"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFM8905364.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="ACHA44_002103"
     CDS             3798..4025
                     /locus_tag="ACHA44_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFM8905365.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="ACHA44_002104"
     CDS             4030..4572
                     /locus_tag="ACHA44_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFM8905366.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="ACHA44_002105"
     CDS             4799..5515
                     /locus_tag="ACHA44_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8905367.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7410)
                     /locus_tag="ACHA44_002106"
     CDS             complement(5575..7410)
                     /locus_tag="ACHA44_002106"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8905368.1"
                     /translation="MQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFFFLL
                     LLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVSPVL
                     LIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHKNSP
                     SFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMKKYN
                     TKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTGGLM
                     LSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIAIHP
                     FTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQSNT
                     ETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAVKKF
                     IQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAATLNK
                     EVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVFVTE
                     DSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="ACHA44_002107"
     CDS             complement(7606..8604)
                     /locus_tag="ACHA44_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905369.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8823..10043
                     /gene="sstT"
                     /locus_tag="ACHA44_002108"
     CDS             8823..10043
                     /gene="sstT"
                     /locus_tag="ACHA44_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFM8905370.1"
                     /translation="MSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSCLKAIAPI
                     LVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPITLQETVSE
                     KAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSESTKELVDQI
                     SIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLFVALVVYP
                     FLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKESYSISIP
                     LGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGVAGGSLLL
                     IPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELGARKRSGE
                     KIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="ACHA44_002109"
     CDS             10190..10825
                     /locus_tag="ACHA44_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HFM8905371.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="ACHA44_002110"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="ACHA44_002110"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFM8905372.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..13514)
                     /locus_tag="ACHA44_002111"
     CDS             complement(11901..13514)
                     /locus_tag="ACHA44_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905373.1"
                     /translation="MDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNV
                     WSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVS
                     YFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYI
                     RDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVL
                     TFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLG
                     LIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSL
                     FIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAV
                     AIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNT
                     VLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYL
                     SIVTLILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4530 a         2135 c         2631 g         4220 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgcctggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt
    13261 aaaacaagat ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga
    13321 taaaaaatat tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc
    13381 catacattat ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta
    13441 tgatagattc ccttagacat gaatagaggg atgacgtaag caaaacttag aaataacata
    13501 aaaatgatat ctatta
//
LOCUS       DAYYGS010000073        13427 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000073 DAYYGS010000000
VERSION     DAYYGS010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.073"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            69..1496
                     /locus_tag="ACHA44_002112"
     CDS             69..1496
                     /locus_tag="ACHA44_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905374.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1588..1953)
                     /locus_tag="ACHA44_002113"
     CDS             complement(1588..1953)
                     /locus_tag="ACHA44_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8905375.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1950..2315)
                     /locus_tag="ACHA44_002114"
     CDS             complement(1950..2315)
                     /locus_tag="ACHA44_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HFM8905376.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2507..2755)
                     /locus_tag="ACHA44_002115"
     CDS             complement(2507..2755)
                     /locus_tag="ACHA44_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905377.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2754..2882
                     /locus_tag="ACHA44_002116"
     CDS             2754..2882
                     /locus_tag="ACHA44_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HFM8905378.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2939..4633
                     /locus_tag="ACHA44_002117"
     CDS             2939..4633
                     /locus_tag="ACHA44_002117"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HFM8905379.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4733..4906
                     /locus_tag="ACHA44_002118"
     CDS             4733..4906
                     /locus_tag="ACHA44_002118"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905380.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            5284..6417
                     /locus_tag="ACHA44_002119"
     CDS             5284..6417
                     /locus_tag="ACHA44_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFM8905381.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6448..6768
                     /locus_tag="ACHA44_002120"
     CDS             6448..6768
                     /locus_tag="ACHA44_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HFM8905382.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6797..7261)
                     /locus_tag="ACHA44_002121"
     CDS             complement(6797..7261)
                     /locus_tag="ACHA44_002121"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905383.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7393..8646)
                     /locus_tag="ACHA44_002122"
     CDS             complement(7393..8646)
                     /locus_tag="ACHA44_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFM8905384.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8675..8992)
                     /locus_tag="ACHA44_002123"
     CDS             complement(8675..8992)
                     /locus_tag="ACHA44_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFM8905385.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(9018..9338)
                     /locus_tag="ACHA44_002124"
     CDS             complement(9018..9338)
                     /locus_tag="ACHA44_002124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905386.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9802..11217)
                     /locus_tag="ACHA44_002125"
     CDS             complement(9802..11217)
                     /locus_tag="ACHA44_002125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8905387.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11234..12550)
                     /locus_tag="ACHA44_002126"
     CDS             complement(11234..12550)
                     /locus_tag="ACHA44_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8905388.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12700..13398)
                     /locus_tag="ACHA44_002127"
     CDS             complement(12700..13398)
                     /locus_tag="ACHA44_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFM8905389.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
BASE COUNT         4255 a         2736 c         2315 g         4121 t
ORIGIN      
        1 tttcaaaaag aaaacatgtc ataatatttc ccatattgtg caaaaaaata caaggcggag
       61 gctctactat gattgaagaa tatattgaga aaaatatttt aagacagctg tttttatgtg
      121 ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt
      181 gcaaaaccac tttactcaat gatatcaata acataaaaaa agaatttgaa gaacagatcg
      241 cttatacaca tcgggaaaag gaccgttaca ctttatattt ttcagaacat atacctcgat
      301 gcaagatcat gcagcaattg tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt
      361 tggaagaaga tgaacctgat tatcttcaat taactgaatg tgaattcatc tctgtcagca
      421 aagcttattc attaaaaaaa caagtattag cttattttaa tgactgcggg atcgaaatcg
      481 accgctactc ccctcgattc actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt
      541 atcgtttagg cggttttaat tcttgggagc tacctgaatc gttttttgaa cgtgccgatc
      601 gtttcattga atccgtaacg gaaaatagtg gcagatttta tgataaagaa aataaagaaa
      661 tcttatcgat tggttttgct atcagtttcc tacgccagca agtgtgtgct gtgacaatag
      721 attcaaaatt tatcgaagaa atcaaaaaac gtcctattta caattatgtc gaatcagcat
      781 gggaaaacac tgatttccaa acctattata aaaaagaaga attcgccttt attttgacac
      841 tattcaatct ttgtaattat ggtttccatt cttatcagtt gattgcggaa gactttcaac
      901 agctacacca agtgtttatt gacaatactc ccgaaataaa agaactagtt gctacctttg
      961 aatctcactt caatcaagaa ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc
     1021 tgatgcgttc ggcttgggat aactatcaat tattcatgcc agaaaaattt tatcttttaa
     1081 atgaggaaca gacaaattta tataaagaag tccagacaat tttttccagc tggtccagcc
     1141 agttacctta tgatctacgg ttgaatccta attgcatgcg ggcttttgtg atcgaattga
     1201 gcggaattct ccgtctgaca aaagaacatc tgactatcta tatcgtcaca aattcggatg
     1261 ttcattatct gatttatcgg gaagccttgg aagctgtaac tactttcgat tttcaagttg
     1321 caccaacaat ttattcaagt atatctgata taaagaagta tgcgcagcag tcctcaaacc
     1381 gggtattatg tgaacgtaca ttatatacac cagatgcggt ccaatatgaa aatattattc
     1441 ctatttcgat caatacgatc gatcgagcta tcatttcagc tgtgcaaaac aaataagggg
     1501 ctgtgacaaa aatcatgtca cagcctctta tttgttgaag atgcccattt ctgtaacaat
     1561 ctcttgatac acgtcaggct cacaacctca cttattttgg acatctacgg gtccgatctc
     1621 tttatttaaa aaataagaaa tgatggttcg gataactacc aacgccgcta gttggaaaat
     1681 atcctgcaag gtaggtttga taatcgattc gatgatccca gctgaaacaa gaatttccaa
     1741 acttagaagt atatagcctc ccaacatctt tttaatagca ttgttctcgc tgactcggtc
     1801 gctttgcact tttgtatgga caaataaata tttcaaaaaa tccttgattg atagacaaac
     1861 accccaaacc aataaacaga tagcaaagat atcaagtccc agtacaagtt tttcgaaata
     1921 tggaagaaga aactgcatca actctgtcgt catcctacct ctcctcttct ttgtttttct
     1981 ttgtcttcta tttcctgatt caagaaataa gaaatgactg ttcgaatgat aataataagt
     2041 gcaagcttca aaatatctgt ccaagtcggc ttgataactg actcgatgat atcagcaacg
     2101 actagtactt ccaagcttaa caaaatataa cttgctagca gttttcttgt atctgcatta
     2161 cgagtattac gttctgcata gtttttcttg acatgagacc aatcaaaaag tttgaataga
     2221 acaagaacga tcccgacaat taagataccg atagaacaaa tatttaaaat agcaatgatc
     2281 cattctagaa attcaattaa atttgagcga aacatatacg ctacctactt tcttttaatt
     2341 tgttcctatt cttttattgt aaaagaaagt taccttgttc gtctatttaa aagttgttct
     2401 aatgataacg gttcgcttaa atttttcttt ttcactctcc catttttctc cctccatcca
     2461 taaatcgtgg aagtatccaa aacaacgtag caagttaaaa taaggcttag tccgtcatca
     2521 gcttttcaaa gaagcactgt ctcggtgctt tttttgtacc caaatttcta aaaatagaac
     2581 catacacctc ctcaaatctt ttcttcttac ctaccctatt tttatttaca ggaaaatcag
     2641 acacttcttc ttttttgtca aaatttcctt tttcttctcg tcctatcatt tggcattcta
     2701 ataaaaacga actatccttg gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta
     2761 aaaaattaat caaagtcggc atcggtttag gtttgcttgc ccttggcgct gcatatctag
     2821 gaaaaaagac aggactattc gaagatgaca gtcatttata tgatgaattc gaatcaatct
     2881 agcatcctat ttagttgaat cactacggac atacttggaa gacattggag gaatcacaat
     2941 gagaaaaagt aaagcaataa tgattggtgc cggtattgcc aatatggcag cggctgttta
     3001 tttgattcaa gaaggcaaat ggaaagggga tcagatcacc ttttattcat tggatgatca
     3061 cggttcaaat gatggtgcac cgaccgacac tgtagtggat gaatattgga ataaaaacca
     3121 tccattagaa aaccaaaaag gctatattgc tcgaggcggt cgcatgctta attaccgtac
     3181 ctatgtagat ttgatggatc tgttaagtcg aataccttct gttactgagc cagggatgac
     3241 tgctgaagaa gatacgagag attttgatgc aaaacaccga acctttgaca aagcacgttt
     3301 gatggaaggt ggcaaaggaa tcattgatgg cggaaaactg ggattcaata ataaagaccg
     3361 tattcttctg actaaactga ttgctatgcc tgattcagaa gaagaaaaac tagacaatat
     3421 cactatcgaa gaatatttca aagatgatcc acactttttt gaaacaaact tctggtttat
     3481 gtgggaaaca acatttgctt tcagaacacg tagttctgct caagaattgc gtcgctatat
     3541 gcatcaaatg atttatgaat tcactcaaat cgaacatcta gtgggcgtca atcgaacacg
     3601 ttacaatcaa tacgaaagta ttatgcttcc tttgatcgag tacttgaaag aacaaggatg
     3661 caaaatcatt ttgaaccgcc gtgtcactga ctgggaattc aaagaaacac cgatgcaaga
     3721 tgaaatcact gtgactggtt tgaaaattac caatacactg actcaagaag aagaatacgt
     3781 tgtggtcgat gaagatacag cagttatttt cactaatggg tccattacag attcagctac
     3841 tttaggagat tacgaaacac cagctgtaga aaatatggat tatggtgcag cttctagtct
     3901 ctggaaaaaa gccagtgaac gtttctacaa cttgggaaat cctgacaaat tctttgctga
     3961 tcgagatgca agcgaatggg tcagctttac cctgacgacg aaaaaccatt tgttgttgaa
     4021 tgaaatcacc cgtatcacga ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac
     4081 cacaccgatc acgccgttag gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca
     4141 accgcatttt acgtctcaga aaccaaatga aacagttatt tggggttatt tcctttatcc
     4201 aagacgacgt ggcgaatttg tggataaaca gtatattaaa atgaatggaa aagaaatgct
     4261 agaagaactg attggacaac tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa
     4321 agaaacagaa attttcgaca gcgtaataaa taatatccct gtctatatgc cttatgcttc
     4381 cgcgttgttc aataatcgtg ccaaaagcga tcgaccaaaa gtcattccta aacattcgac
     4441 caatctggcc tttacaggag aatttgtcga gcagccttat cagatgatct ttactgaaca
     4501 aagtgctgta cgttctggag aaatcgctgc tttccatttt gcgggtattc ctatgtcccg
     4561 tcttgtcaaa acaccgcgtt acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa
     4621 aatgtttgaa taatcaagag gttgtgaaaa agctgccctg catcaagtaa taagaacaac
     4681 caggctaaaa cagcttctta tgtttttgat ttggttggac ttattaccgc agatgcaagc
     4741 ttttgaacac cgtttaccaa gaggttatga aaaagctgcc ctgcatcaag taataagagc
     4801 agccaagcta aaacagcttc ttatgttttt gacttggtta gacttattac cgcagatgca
     4861 agcttttgaa caccgtttac caagaggtta tgaaaaaggc gtttagtttc gagtatgaag
     4921 aaaaattttg tgaaaatggt tcctcacgtt tttgcataaa tttgacttaa taccgagaaa
     4981 ctgatccctt ctgtcataac ctctttttat ttatgtctgt ttctttttga aaacaactat
     5041 tttttcaacg tttactctct tgccaatcca acatcatcta acggatacaa ctatttcaat
     5101 gattaccatt acactaaaaa atcggaaaca cacctctccg aacacaaata cataagacta
     5161 tagaatgttt catttaagaa aaaaagcgtt tcattatcaa aaaggtaaga tctcctttaa
     5221 aatgaaatta tctgtagtac taataatgca gacaatttta tgaatctgaa aggagcatct
     5281 ccaatgaaag cagtcacttg gcaaggaaaa cagaaaatgg aaatcagaaa cgtagatgat
     5341 ccaaaaatat tagaaccaac agatgcaatc atccgtatca cagcaacagc catttgcggc
     5401 tccgatctgc atctttacca tcatggtgaa gcagttttgg aaaaagggta tgttgtagga
     5461 cacgaaccaa tgggaatcgt tgaagaagtg ggatctcaag ttcaaacatt gaaaaaaggg
     5521 caacgtgtgg ttatcccttt taatattagt tgtggtcatt gtcatttctg cctgaatcac
     5581 atggaaagcc aatgtgatga atcaaatcca gatcagttat atggcgggct tttcggattt
     5641 ggcaagttga acggtaatca ttggggtggt caagcggaat atctaagagt tccttatgca
     5701 gactctactt catttgttgt accagataat gatcttcctg atgaaaaagt tttatttcta
     5761 tcagatattt tgcccacagc ttattggagc gttgaaaacg caggagtaaa aaaaggcgat
     5821 acagttgtga ttctaggttc tggtccagtc ggtctattcg ctcaaaaatt cgctgttatg
     5881 gctggagcag agcgcgtaat cgcagtagat ccagtacagc accgtctaga taaagcggct
     5941 aattacaatg gggttgaaac ttatttctta gaagacacgg cacaagctgg aacagaattg
     6001 tatgaactgg ctaaaggtgg cgcagatgtc attatcgatt gtgtaggaat ggacggatta
     6061 gagcctgtca aagaaaaagc gaaaaattta gtaagcttac aaagcggcac aatctctcca
     6121 ttacagatgg catcgcaagc tgtcaaaaaa tttggaacag tccaagtaac tggtgtttat
     6181 atgactccag cttcttctta cccgctgcaa gaatttttta tgcgggatat cgctgtcaag
     6241 catggacagg caccaattat ccatctaatg ccaaaaattt acgcaatgat tgcagaagga
     6301 ctattcgatc ctacacaaat cattactcat tcgctgccac tatcagaagc agcacaagca
     6361 tatgagatct ttgacaaaaa agaagataaa aatataaaag ttgttttgaa accttgaatt
     6421 cagtaaaaaa tacaggtgat tttctttatg cccaaaatca aacgaaatac ccatgagatc
     6481 gaaataggaa aagaagaaga tatcgaaata cgaggacgtc gcttccgctt ctatttccaa
     6541 aatcgttaca cattgatctc actggctgtc gatttattaa ccgggatttt ctatattttt
     6601 ggaagccttg cttccttaac taaaatcccc gatcgatatg gcacctattt ttatttggct
     6661 ggtgctattt tcttgacgat tcgtcctatt ctcagaatcg taaggaacat ttttatttac
     6721 gacgaaaaaa aagcaatgaa gagaacaagc aaaaaaaaag aggcatgaga atttttcaaa
     6781 tctcatgcct cttttttcac cctatcgtat cactttgata cagacgagcg gtcgtcccac
     6841 tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg ttcataaaac gtggtatgat
     6901 ctgtaattaa gtaaagtttt tcgtacccaa gttgatgcat ttcttttctg atttccgcta
     6961 atatccgctt ggctatcccc tgcttacgct tttccggttc aacaaacagg gcacaaagat
     7021 ttggtgtcag atcagggcgg ttatggaagt cgttttcaat gacgcccgct cctccgacga
     7081 tggtttcttt ttcatctaag accacatacc attgaggaat actttcctta ttagcgattc
     7141 cagtacgaat actttcctca tacacaacag caggaactcc ccattttgat gaaaaccaat
     7201 tcgcgctttg ttttactaat tctgggtgtt ctcttaattt gatccagtta tatgtaacca
     7261 atgtttaaac ctccgactaa aataaaacgt ttacaaaaat tatacaagtc tttatgaaaa
     7321 ttagcaatga caacaatagt gatgtaaaaa aaaaaacgga aaacaatcac ttgttctccg
     7381 ttttttgatt aatcaagcat ctaaagcttc ttttcttctt tcttggatcg tagctacctt
     7441 gacgaacggt agatagatca aaatagaaag aaccaaattg acagcggcta gtacaccacc
     7501 tgcaatactt tttgttgcca atacaccgcc aataattggt ggagttaccc atggaggcat
     7561 aacggtagct gctggcacta aacctgtaga agtcgcaaag taagcaacac taactaagac
     7621 cattggtgtc aaaataaatg gaatgaacat gatcgggttc aacacgatag gtaacccaaa
     7681 catcgttggt tcattaatat tgaagacacc tggcgcaatc gacaagtttg ttacaaccat
     7741 atatggtttg tttttacgtc ctactaaata aatagctaac agtaaaccta aagttgcacc
     7801 tgttcctcct aagttgacaa agctgtcaaa gaaaggtttg ttcacgataa acggtgcgct
     7861 atgtccagct gcgattgcat taacgtttgc ttcaatggct ggcgcattga ttgtttgcat
     7921 caaaggatcg accatattcg ctccgtgtaa accaaagaac cataagaatg gtgtgataaa
     7981 tgcaagcagt aaggcagatg gataagagtt tgcaagtccc ataaatggtt cttggaccac
     8041 tgtatagaaa gaacctacga tatcatcaac accgaatgca gcaaaaattg cagcaactaa
     8101 agcaaacgca gagatagtga tcattgctgg tagtaatgaa gcaaatgctt ttgcaactgc
     8161 cggtggcaca ccatcaggca ttttgatgat caatttttca tttccgctta aacgtacaaa
     8221 gatttctgtt gaaataatcc caacaataat agcaataaac aaaccgtttg agctcatacc
     8281 taatgctccg cctaaagcga agaaagagcc taaagaaatc acacctgcgg aaacaccgtc
     8341 ttttcggtag ccgttagcta aatgataagc aagcaagaaa gcaatcaaga cagaaagaat
     8401 ggcaaatgta ccgttccaag cagcagcccc gaatcctttc catgattcac caccaaaaat
     8461 tgagttcatc aattcttgga acccgggaat cggtaagtta ttgatcatta ctgctaatgc
     8521 tcctaagatc attaacggca tactcaccat aaatgagtcc cgaatcgcaa ctaaatgccg
     8581 ttgtgcacca atttttgatg ctacaggtat aaaatgtttc tccataaatg caacaaattt
     8641 atccataaat cttccctctt ttctttactg tagattacgc gttcaatcga cgatacagat
     8701 caatgatctc ttttgctaaa tctgtaaaag ctatagatgt catcagatga tcttgactgt
     8761 ggaccgtcaa aagcgtgact tcaacgtgat ttccttgtgc ttcttgtgtc agcatctctg
     8821 tttgagagtg atgtgcttgg actaaagatt cctctgcatc tttgattttt tcatctgcta
     8881 aagtgaaatc accttttttc gcagcagcaa tcgcttccat tgcatcgctt tttgcatttc
     8941 cgccaaacat gatcagaccc atgatggctt ctagattttt ttgatcttcc atttattgta
     9001 tcctccgata atttttatta tttgtttatt agtttttctg cttggtcgag tactttttca
     9061 ccattcatca tgccatagtc agccatattg attacatcta aaggaatgcc tttaggtgct
     9121 agcttttcag caaattgatt ttgcataaag cgtacttgcg gtcctaaaag taaaacgtct
     9181 acatttttag attctagttg attatccgca tctgaagcag atacagcaaa gatgtctgct
     9241 tccattcctt ttgcttctgc tgccttttgc atttttgtga ctaggagact tgtactcatt
     9301 ccagctgaac atacaagcat gattgttttc ttttccattt taaacacgtt cctttctata
     9361 cttacacttg tatataagca agtagcgtgc caactttaaa agcgctttaa aaagttgata
     9421 tcatcacatt taaggtctat accaagttgc atacacacta aaaaagaaag cgcacactaa
     9481 acacacaaat taatttttat tacctttttt cttttaacgt gcacaaaaaa agttgtgaca
     9541 caaaacttgt cacaactttt ttcaaataaa cggtattcaa aagcttgcat ctgcggtaat
     9601 aagtccaacc aagttaaaaa cataagatgt tgtttttact ttgctattct tattacttga
     9661 tgcaggacag ctttttcaca acctctcaat aaacggtgtt caatcagctg accctgggta
     9721 ttagctcaaa acgctggatt cacaacctct tttatttcaa ataaccagta aatacaatga
     9781 acatctacaa aaaacttcta tttatttata gatttcgtca ggcaaatcca attctctctt
     9841 atcaatcaaa gtctgatacc acgaagcaga ttttttcaat gttctgcttc gttcatcgtt
     9901 caattcgatt cgaactaatc catatcgatt cttaaatgca ttcattggtg acacacagtc
     9961 agtgaatgcc cataacatat agccttcgca atttgcgcct tcttctgttg ctttgatcaa
    10021 gtaatgcaaa tgctgtccga taaattcgat acggtaatca tcttgcacga ttcctttctc
    10081 atccatatac tgttcttcat attctcttcc cataccgttt tctgttatca gccaagggat
    10141 attatgataa ttttctttta gatacatggc tatatcatac atgattttcg gatagatttc
    10201 ccatcccctg gaattattca ttttcttccc aggtaaatca aagacttcat aatatcgttc
    10261 aggatggaaa ggtgtctctt cattccattg ataacgaggg gcgcgaacgc gtttaggata
    10321 atattgattg attccaagga aatctactgt atgatgctga atcgtttcta aatcctctgt
    10381 tgcaggatcg aaataaatcg attgtttttg acacaactcc aggaactctt ctgtgtagta
    10441 tccttttacc atcggatcaa aaaatactcg attataaaac aaatcataca tataagcagc
    10501 tttttggtct tctggagaat tggatcgagc atagaccatt tcaggattta aaatacagcc
    10561 aatccttcca ccataatttc cgctgcggta aatctctact gctttagccg ttgctaaaac
    10621 tttatgataa ttccacatca tccacttttt tgtgttttgt tcatgcggcc agcgaacagc
    10681 atccaaatag caacgcgttt ggggaacgat tggttcgttg aatgtaaacc agttttttac
    10741 cctatcaccg taacgatcaa aggcaatttg agcatactta gcataaaaat caactacttt
    10801 tcttgaagac cagccatcat atttttccaa aagaaaagct gggacttcat aatgttccaa
    10861 acaaatcatc ggttctacgt ctgcttcgat caaagagtcg atcatttcac ttatatgctg
    10921 agcatattct tcatctacta tacagttctc ataatcagta aaaaaacgag cccaattgat
    10981 agaagtccga taatgtgtca gatggatgga tttcatcaga tccacatcct gctggtattg
    11041 ttccataaaa ttagttgcta cggctgggcc atacccatta tgccaaacaa aatgttcatt
    11101 tttataccag ctatctataa acgaatcctg tccttctttt tttcctttcc aaccttccgt
    11161 ttgccaagca gaagatgcag caccaagaat aaaatttttt ggaatagtaa ttttcatgat
    11221 ttcctcctgt tttttagatc ccatcatttt ccacacgatt tgctactttt acaaatggca
    11281 gatagatcaa aatagaaaca ataatacaaa caagttgcgt aataactgca ccgatatttc
    11341 ctgctgttgc cagataagca ttgacgatcg gcggcattgg ccaagggacc attacaactg
    11401 ctttcgccgc aaatcctaaa gcggttgcaa aataaccaat cgttccagtg ataagaggcg
    11461 ttaagataaa cggaatagct aaaattggat tcagcatgat cggcatacca aaaataactg
    11521 gttcattgat gttgaagatc ccaggagcaa gggataactt ggtgatttgt ttcatatctt
    11581 cccgttttcc aactaacaat actgcaatca gtaaaccgat cgtgactcca gacccgccca
    11641 tactcatata catatcccaa aatggcatag taataatgtt cggtacttct tttccagctt
    11701 caaaagcttc cgtatttaca gcaattgccg ctaaaagcaa cggttcccgt atcggcttca
    11761 ccatttgatt tccatggata ccaatcaccc aaaagacttg cgcaataaac atcaatagca
    11821 aaatacccgg taacccttgt acgacacctt ctaatgggcg ttgtacgaca ttgtaaatga
    11881 tatcataaag gtacattcct gtaagttctt tgatgataaa tcccgcagcc gcgatacccg
    11941 atacagtcag aatcgtcggg aataaagcag aaaaactagc agaaacattc gttggcacgg
    12001 tatcaggcat tctcagctgt agtttcttct gtttcctcaa ccagcaataa agttcaatag
    12061 aaaggatgga gaccaacatt cccaaaaata accctttcgt atctgtatat tgtttagcca
    12121 atacgttttc tactataaca gtctgatttt cattcactaa ttcataaaca gttggattaa
    12181 cagataaata agacatcact gcgatgatac cagggaatac accatccact ttattcagct
    12241 tggctaattc gatccctatc agaaaaacag catagatggt caagaaactt agtgtgacat
    12301 agctgatggc agatgcgata ggctttaatt cttctaaaaa acgcaagaaa cttatctgtg
    12361 ctaagccatt ttccgagtca aagaccattg ctgaaaacaa tgtgccaaaa gcacccgtaa
    12421 taattaccgg aatcaatgca gcaaatgcgt tttttatcac catgatatac cttaaattat
    12481 tgattctcgt tgccacgttc cctaaaacat tggctaattt atccataaaa ctcattccag
    12541 atcccctcat tcctgcttct tctccttgac gtcatctctt tttgtctatt agtcagcgct
    12601 tacatttcga tgaacagaaa ctatttactc ctctttttta ccaaatataa aacaaaaatt
    12661 catctgatgt atttatcata aaatacatca gatgaactgt caattagttt ctttttctcc
    12721 tattattttc tgtaattctc tccgatttat tgccatatgg acagtcattg catcaaacgc
    12781 agcaagtgca ttccgccttt cgatggcctg aacgatctct gtatgaatat cgatcgtctc
    12841 gattttcaga tgctggatat cataattttt attaaacaaa gcaattgatt cattaatgat
    12901 tgggataata tgcagcaagg ctacgtttcc gctagcttct gcaatcatcg tatgaaaatc
    12961 gatgtctaat tgaacatgtt cttgtgtgcc ttctcgaaaa gaagtttcaa tcttttgcgt
    13021 taattttttc atttggacga tttgattctt cgttgcatat tccgctgcca aacctgccat
    13081 cttaggctcc aataaatatc tcagttcaaa taagtctcgt atcatccgat cctgatcttc
    13141 aatcaaagaa aaaccgagtg ggtcattggc aatccctcgc ttatcactga caaatgtccc
    13201 cgctccttgc cgaacttcaa ggatattcct ggaagctaga gcccgcacag cttctcgcac
    13261 cgtgcttcgc cccacctcta aataatcgga caattcatat tcattcggca gtttatctcc
    13321 tacttgcaac tgcatttcct ttaaatatct aaggatcttg tcagtagtta cttctactag
    13381 ggttttattt tcttccaaac cacatacctc cttttcatct gatgtat
//
LOCUS       DAYYGS010000074        13085 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000074 DAYYGS010000000
VERSION     DAYYGS010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.074"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(78..2198)
                     /locus_tag="ACHA44_002128"
     CDS             complement(78..2198)
                     /locus_tag="ACHA44_002128"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HFM8905390.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2428..3606
                     /locus_tag="ACHA44_002129"
     CDS             2428..3606
                     /locus_tag="ACHA44_002129"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HFM8905391.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA44_002130"
     CDS             3622..4380
                     /gene="aroD"
                     /locus_tag="ACHA44_002130"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFM8905392.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA44_002131"
     CDS             4403..4888
                     /gene="ybaK"
                     /locus_tag="ACHA44_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFM8905393.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4959..6212)
                     /locus_tag="ACHA44_002132"
     CDS             complement(4959..6212)
                     /locus_tag="ACHA44_002132"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8905394.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA44_002133"
     CDS             6328..7677
                     /gene="gdhA"
                     /locus_tag="ACHA44_002133"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFM8905395.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7789..9135
                     /locus_tag="ACHA44_002134"
     CDS             7789..9135
                     /locus_tag="ACHA44_002134"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFM8905396.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9443..9829)
                     /locus_tag="ACHA44_002135"
     CDS             complement(9443..9829)
                     /locus_tag="ACHA44_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8905397.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(10097..10246)
                     /locus_tag="ACHA44_002136"
     CDS             complement(10097..10246)
                     /locus_tag="ACHA44_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905398.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            10708..10920
                     /locus_tag="ACHA44_002137"
     CDS             10708..10920
                     /locus_tag="ACHA44_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905399.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(11198..12997)
                     /locus_tag="ACHA44_002138"
     CDS             complement(11198..12997)
                     /locus_tag="ACHA44_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HFM8905400.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFYSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4292 a         2353 c         2577 g         3863 t
ORIGIN      
        1 tattctaaga tttctcttta ccaacatcat ttgacaaaga gcctaatttt gtcgctgctc
       61 cttttaaatt tattttatca ttcggaagaa cttgtggtcg ttccgtgaag ttcttggtac
      121 gtttgcgttt tataaagatc aacagttgat tgtcccttgt gttcactaaa gacactagtt
      181 gacttgtcac caagagattt ttcagcttct tttaatttct ctaagccatt cttgtagtca
      241 taatcactgg tatcaattgg gtctaatcca ctattcgtat agaaccgaag aagatcacca
      301 ttattgattt ggtcagaaac agctaactgt ttatcaactt tttctttcag tgaatcaatt
      361 tcattctgta cttcctcagt aggttcagta atagcttcac cagtttcgtt actatagatc
      421 gtaccaccgt agtaagtata ttttggtgta acaaaatcac catcacggaa agaaacaagc
      481 tgttcatgat ctttcgagaa tagatcttgt cctaactgaa tataattttt tgtatctaca
      541 cctagtaagt gaagtaatgt aggcaaggca tcaacttgtc cgccgtaagt gtgattgatt
      601 ccgccctgtg tttgtccagg gatatggatc atataaggga cacgctgcat ttgtgcattg
      661 tcgtagtttg tccaagtaga acttgttttt ccaactaatt cagctagatt tttatttctt
      721 gaattcgaga caccgtaatg gtcaccgtaa aggacgatga ctgaattatc atacaagccg
      781 ctttctttca aataattgaa gaattcttgt acggcggtat ctaaataatt ggctgtcgca
      841 aagtaaccgt tgatcgtttc atcatttgta ttggctaacg ggaatcctgc ttcgtcattg
      901 gtgaattttg aataaggata atggttagat acagcaatga attttgaata aaatggttgt
      961 tgcagacgtt ccaagtattg tacggattgt tggaaaaatg gtttatcatg caacccgtat
     1021 tggaacgaat tgtcatcatt aacatcataa taagaagaat caaagaaata atcatatcct
     1081 aaattcttgt atgtctcgtt tcgattccag aatgtaccag cgttcccatg gaataccgca
     1141 ctagtgtaac cttgtgtttg acttaaaata tcaggtgcag cttggaaagt attttttccg
     1201 cctaattgtg tgaacaagga gccttgattc aagccaaaca atgaattttc catcagcgtt
     1261 tccgcatcgc ttgtttttcc tgcttttact tgatggaaaa agttgtcaaa actgaacgtg
     1321 ctgttgctgt ggtaaagact attgatgaaa ggtgtaactt caagctggag accattctca
     1381 tctgtcaatt tataatcgat caggaattgc tggaagcttt ctaaatggat ataaatcaca
     1441 ttttttcctt tagcgagccc gaacaaatca tcatttggtg ctgcataatg ttcttttaca
     1501 taattttcta cttctttcat atcattggga cttgcttctg cccgcacttg gtttgtctgg
     1561 taagtttgaa caccgtcata aaccatgaaa gcattcaagc caagatattt gaccaaataa
     1621 tcacgagaga atgttcgagt cagcaattct ggacgatcag cttccgccat aaaaagattt
     1681 cctgagaaaa tcataactgc taatgtagag atcgcaaaag ccattcttgc tcggatcggg
     1741 cgagggtcca acttgatttt ttttgcaatc agcagaacag tcaagataat aaaatcgatc
     1801 caatagatca catcatatgg gcgaaacagt cgaatggcac tttctcccag accactcgct
     1861 actttcccag cacctaaaat cgtgttgatc gtaatgaagt ccgtgaattc tcgataataa
     1921 gtgacattcg aaaaaacaag caatgtcaaa agaaaataaa tgaccatcat cgtgatataa
     1981 gcaactctag ggcgtctgat ataaagagca attccaagta ataacaatgt agttgcaata
     2041 gggttgataa acaagataaa gtattgaatg ccactttcga ttccgagatg gaaatcaact
     2101 aaataggcaa aaatcgtttt caaccaaagt aaaacaacta gtaaagaaaa aaatccgagc
     2161 ctcttattca aaaaggcagg tgtttttata catttcaaga taatcttcct ttctgcactt
     2221 cttaatcaca gaagtgtcat gaaaccatgt tacacagctc acttattctc atttatttta
     2281 ctagtatgac aatatagtgt caattgaaac cagaagttta tgctgtttca ttaaggtttt
     2341 ctttaagctt catgaaaaaa tgagcagtaa gacaagtcct atgttttttg ttatacttgg
     2401 gaagagaaag ctaaagcagg tgaaggaatg aaagtaaaag taaaaaaaag agcaagcaat
     2461 cgattgaaaa aaggatatcc actgatccaa aaagaagacc ttgctcatcc ccaaaaagaa
     2521 accacgcaat gggtggaatt agttgattcg caaaaccaat ttatcgcaaa aggatatcta
     2581 ggaaagcaga ataagggaat cggttgggta ttgagccagc agaacatccc atttgatcaa
     2641 tctttttttg aagaattttt tgtgaagtca aaagaaaaaa gaactttctt tttttcagat
     2701 gattctacta ccgcttttcg tatattcaat ggagaaggag acgggatcgg cggtctgatt
     2761 gttgattatt atgcaggtta tgctgttttt tcttggtata acgaaacgtt atatgcacat
     2821 cgtgaattat tgattcaagc ttttcgtgaa atctacccgg aaatcgtagg aggctatgag
     2881 aaaattcgat ttgaatcagt aaatctgcct gagagccagt tcatttacgg aaaagaggca
     2941 ccggaaccct tgatcgtaaa agaaaacgga gtaaaatttg ccgtttattt aaatgaagga
     3001 ttaatgactg ggatcttttt agaccaaaaa gaagtacggg gatcattagc agaaggtttc
     3061 gcttctggga aaacagtttt gaacatgttt agttataccg gcgccttttc tgttgctgcc
     3121 gcaatgggag gggcaatcga aacaacaagt gtcgatctag caaaacgcag tttaaaaaaa
     3181 acacaggaac agtttgaagt gaatggtttg gatgtcgatc agcaaaaaat cattgttatg
     3241 gacgtattcg agtatttcaa atacgccaaa agaaaacaat ttttatatga catgattgta
     3301 ttggatccac caagttttgc cagaaataag aaaaaagtct ttcgcgtagc aaaagattat
     3361 ggggaactgg tcaaggatag tctggatatt ttagcagaag acgggatact tattgcgtct
     3421 acaaatgctg cgaatgtctc gatagaacaa ttccaaacaa tgattgaaga ggaattccat
     3481 gccaaaaatg tttcttttga acaacttgct ttatatcgtc tgccaaaaga tttccagacg
     3541 acggatacct ttttagaagg caattatctg aaagtgtttg tttatcaaat taaaaataac
     3601 cattagaagg agcgatgcag aatggtttgg gtaaagggat tgaatatagg aagcggtaga
     3661 ccaaagattt gtgcgcctct tatcggggcc gtccgagagg aagtattacg tgaagcagtt
     3721 cttgcacgtg aagcaggggc cgatttagtc gaatggcgag tcgatcatta tcgtgaagtt
     3781 ttttcttttg atgaagtagc agaaacattg gttcttatcc atgagatgct tgatggactg
     3841 ccgttattat ttacgtttcg taccttagct gaaggcggag aacaagacat atcgatccgt
     3901 cagtatcgcg acctttatga acatgtgata cacacaggat tagtagatat ggtcgatatc
     3961 gagttgttca aagtagaaag tctaggaaaa agactgatag aggaaatcaa gtctttaaac
     4021 attcctttaa ttatcagtag tcatgatttt aaagaaacac cagcagaccc tgtcttgctt
     4081 taccgattga atgttatgga acattttgga gccgatatcg gaaaactggc agtaactccc
     4141 aataatgaac gagatgtttt gcggttgatg gagttgacta gacgggcaaa tgcttttgtc
     4201 tccatgccta ttattacgat gtccatggga aatcttggta aaatttctcg tctggctggc
     4261 ggaacgacgg gttccgttat gacatttggt tccttaagca gcgattctac ttctgctcca
     4321 ggacaattac cggtcaaagc attgaagcaa attattcaat tattaaatga accgttgtag
     4381 aacaagtttg aggtgatatt ttttggcaaa gaaaaaaata gtaaaaacga atgcaatccg
     4441 tatggtcgaa caaaaaaaga ttccctatac agagcacgaa tatgagtggg acgaaagcca
     4501 cctaagtgcg tcatcggtcg ctgaacagct tccagaaagc caatctcata tatttaaaac
     4561 actggttgct gtgggcaatg tgacggggcc tcttgttgct gtgataccgg gagaagccga
     4621 attgaatcta aaaaaattag caaaagtttc agggaataaa aaagtagaaa tgctccattt
     4681 aaaagatttg gaagcaacga ccggatacat acgtggtggc tgctcgccta taggtatgaa
     4741 aaaactattt cctacttatt tagatcaaat tgcagaaagc tatgaacaaa tcattgtctc
     4801 ggctggcaga agagggctac agatggagtt agcacctcaa gatatctgtg cattaacgag
     4861 cggacaattt gcagatataa aacaataaat catacgtata aaagaaagaa ggaggccggg
     4921 atgttgtttg tccagtctcc ttctttcttt tttatacatt atgctctttg cccggcattc
     4981 attcctgcta catgccctgt agcgaaagcc gctgtgatat taaagccgcc agtataacca
     5041 ttgacatcca gtaattctcc cgcaaaatac agaccatcaa tgattttact ctccaatgtt
     5101 ttaggataaa tttcctttag agaaacccct ccgccagtta caaatgattt ctcaatagga
     5161 aaagttttgt tgattgatag cttaaactct ttgcatagtt gaacaaaatc ttgaatttgt
     5221 ttttgagagg cttgttgtcc tgtcagatga tccatttcaa gccgctcaag aaagaaaact
     5281 aacaatcgct caggcaatat accatgccaa gcattgacca agttcttttt ggttgcttta
     5341 cttttttctg tcaatatatg aataagctct tgtttggttt gtgtagggaa acaatccaga
     5401 gaaagtgtca ctggttctcc cgttttctct aattctttat tgacaaaact tgaacaacgc
     5461 agtgcagccg gaccagaaat accgaaatgg gtgaaaagta aatccatcgt atgttcagtc
     5521 aatacacgac cttttttatt taaaacgcgt aaagtgatgt cttgtaaaga aagcccttga
     5581 agtgttttct cctgaatata cggttcatcc gatatcaagg gggactctgt cgcataaaga
     5641 ggcgtcaccg tgtgacccac tcgcttcacg atcttgtacc catctccagt agaaccagta
     5701 gatggataag tccgtccacc agttgttaag ataatggcag gagcatggaa ctcttcgaat
     5761 tccgtgcgta taccgtaaat tttatgttct ttgtggatca atttctctac tcgagtagag
     5821 aatagaagcg tcacatctag ttctttcaga cgattgagca atgcttcgat aatcgttttt
     5881 gatttatttg ttacaggaaa catccgtcca tgatcctctt cttttaaatg tacgccttgt
     5941 gattcaaaga agttcatgat atcgaaatta ttccattgcg caaacgtact atataaaaac
     6001 tttccatttc caggaatatg tgcaatcaaa tcatcaacag gacgattgtt cgtaacattg
     6061 caccgaccac cacctgtcat cagtagtttt tttcctgctt ttttgttttt ttcgatcaag
     6121 agtacgcgtg cgccttgttc agctgcactg atcgctgcca tcatcccgct tgttccagcg
     6181 ccgactacga tcacatcata cagtttttcc attccctcac ctcaaggaaa gtgtagcata
     6241 tctgaagaaa gaaaacagaa aaaatgtttt gagccaaaag agaaaatgag ctaaaatgag
     6301 ggaagataag caaaggagcg tggaacgatg acaaacgcaa cagaatatgt tcaagcaatt
     6361 caagaaaagc tacatcaaaa ggatcaaggg caagaagaat ttttacaagc aatcgatgaa
     6421 ttcatgccca cggttatgtc ttttttggac actcatcccg aatatatcga aaagaatatc
     6481 ttagaacttt taacggaacc agaaagagtt attcaatttc gagtgccttg gcaagatgat
     6541 gaaggaaact ggcgagtaaa tagagggtac cgtatccaat acaactcagc tatcggccct
     6601 tataaaggtg gattgcgctt ccatcctagt gtcaatctaa gtatcttgaa atttttggct
     6661 tttgaacaga ttttcaagaa tagtttaacc ggtctgccaa tcggtggagg aaaaggcgga
     6721 agtgacttcg atcctaaagg aaaatctgat aatgagatca tgcgtttttg tcaaagtttc
     6781 atgctggaat tgtcaaaaca tatcggccca tcgattgatg ttcctgcggg agatatcggt
     6841 gtaggagcta gagaaatcgg ctatttgttc ggcgaatata aacgattgaa aaaatatgat
     6901 actggtgtgt tgacaggtaa acctttggat ttttggggaa gtaagatacg tacggaagct
     6961 actggttatg gattagtgta ttatgtcaaa catctattga acgaagaaaa agattcattt
     7021 aatcaaaaaa cagtttttgt ttccggaagc gggaatgtag ccatttatgc aatcgaaaaa
     7081 gtgcaagagc taggcgggaa agttgttaca tgttcagatt ctagcgggta catctatgat
     7141 ccagaaggaa tagatgtgaa actattgaaa gaagtcaaag aaatcaatag acaacgtttg
     7201 acggaatacg caaaccaacg tccttctgct atttatcatg ctggagaatc tgtttggaca
     7261 ttgaaagaaa aagcggatat cgctttgcca tgtgctactc aaaatgaaat agacgaaagc
     7321 ttggccgaaa tcttagtaga aaatgggatc aaaatcgtag cagaaggcgc taatatgcca
     7381 tgtacagtaa aagctgtttc tgttcttcat gaagcaggcg tgtggtattg tccaggaaaa
     7441 gctgctaatg ctggaggagt agcagtatct gctttagaaa tgagtcagaa cgcccagcga
     7501 ttgacttggg aaagagagca agtggatgag cagctggatc aaatcatgga aacgatttac
     7561 catacgtgtc gccaaacagc agccgagtac ggcgacgaaa aaaatctgct tcttggagca
     7621 aatgttgctg gatttgaaaa agttgccaaa gcaatggcag cagaaggttt agtttgaaaa
     7681 atgaaacttt cttggaaata ttgtcagcat tttgtttatt tgcttgaaaa ataataaaaa
     7741 aaaacgtaaa gtattacctg tagagtaaac ctataaggag gagaacaaat ggcacacatt
     7801 cattttgact attcaaaagt agcaccgttt gtcaacgaac aagagttagg gtacatgcaa
     7861 agccaagtaa cagcagccca caacgaatta cgggaaggta ctggcgcagg aagcgacttc
     7921 cgcggctgga tcgatttacc aacgaactat gacaaagaag aatttgcacg tatcaaagaa
     7981 gcagctaaaa aaatccaatc tgattctgaa gtattagttg ttatcggtat cggtggttct
     8041 tatttaggcg ctcgtgcagc gatcgacttc ttgaatcaca cattcttcaa tttattagat
     8101 agcaacacaa gaaaagcacc acaaatcttc tttgccggaa attcaatcag ctcaacatat
     8161 attgctgact tgatcgaagt aatcggtgat cgcgactttt cagtaaacgt tatttcaaaa
     8221 tctggaacaa ctactgaacc agcgattgcg ttccgtgtat tcaaagagct tcttgttaag
     8281 aaatatggac aagaagaagc aaacaaacgt atctacgcta caacagataa agcaaaaggt
     8341 gcagttaaag tagaagcaga tgcagaaggt tgggaaactt tcgtgattcc tgatgacatc
     8401 ggcggacgtt tctctgtatt aactgctgtt ggtttgttac caatcgctgt tagtggtgca
     8461 gatatcgatg cattgatgca aggtgccgct gatgcaagca aagcatactc aagcgacaaa
     8521 ctagaagaaa acgaagctta ccaatacgca gcaatgcgca acattcttta ccgtaaaggc
     8581 aaagtaactg agttgttgat caactatgaa ccaggaatgc aatacttctc agaatggtgg
     8641 aaacaattgt atggtgaatc tgaaggaaaa gaccaaaaag gcatctatcc ttcaagcgca
     8701 aacttctcaa ctgatttgca ctctttagga caattcatcc aagaaggacg tcgcaatatt
     8761 tttgaaacag ttgtgaaggt tgaaaaacct cgtaaatcaa tcacaattcc tgaacaagca
     8821 gaagatttag acggtctagg ctacttgcaa ggaaaagaag tggacttcgt aaatacaaaa
     8881 gcatttgaag gaacattgct tgctcataca gacggtgacg tgcctaactt gctagtcaaa
     8941 attccagaaa tggatgctta tacattgggt tatacaatgt acttctttga aattgctgta
     9001 ggtatttcag gttacttgaa tggaatcaac ccattcgacc aaccaggtgt agaagcttac
     9061 aagaaaaaca tgttcgcact tctaggcaaa ccgggctttg aagatcttgc taaagaattg
     9121 aacgaacgcc tgtaagataa gggtttttcg ctagatttaa ataatcagtg atagaaacat
     9181 gttcgcttta aaatgaaaac tggtacccat ttaggtactc aaacatactg catcgtttat
     9241 tcagaataag gggctgtgac aagacgtttg tcacagtccc ttgtttgtat agaaatcgat
     9301 tgattgcctt gctctttagt ttgttttagg actttaagaa cagtgaggct gacttcgaag
     9361 gttaattctg tttttttgtt ttctagaatc agtaagcaaa aaagcactca cttcgagtgc
     9421 ttttttgcga tatattctta gcttaagata ggcctaattt ttcttttaca gatgagggta
     9481 ccttatcgct ggtaattact tttttctcta tgatccgctt ttcgcttgct ttcaactgca
     9541 agatcgttcc ttcggtgtaa gcagaaccat tggcagagtc accagaagta atgacatcga
     9601 aatctaactg tcttttttca ccgttttcag cataagcatc tacgttgtat ttaaaacctc
     9661 ttccaattac ttccccatta tctgctttga tatcagtttc tgcaggcatt ggagctttga
     9721 ctaccgcata ataatcttta cctacataag tagactgata gtagtcccaa cctttccatc
     9781 ctataaaagc aatcaatatg ataacgatca accctaaaat ttttttcatg aaagatctcc
     9841 ttttttattt ttattatatt aaatattata cagataataa cggaatatta tcatcgaaac
     9901 agctaacaaa attgtcataa aaaacgagat ggttgatatt agaacttgtg tttttgttta
     9961 ggtctagaga taaagagaga caatttccaa atatatgtaa tgaatacaaa aagacgtttg
    10021 tcgcggatta ccgttacaaa cgtcttttat tgattttccg agatctcatc agtcgccatt
    10081 tggaatggtt cactccttat ctagattcgt ttaatgctcg gactgcttgt tcgattgtaa
    10141 taccaaacgc aacgttattt tcgttttgtg catcaatcgc cataaaacgg ttagaagttg
    10201 tatcaaaagc tacgcggtat tgcatatttg tttttgtaaa cgtcataaaa ttcatctcct
    10261 ttgtaatgta atttagttct tgaaagtttc ctttaaactt tcgaacttat accaagttta
    10321 gcatcgtacg ttttttgatg caattaaaaa gcatttatca ttttccttat ttattttttc
    10381 ttaaatggtt tagctatttt cttttaatcc gataagtagc ccaatcatca aggttacaaa
    10441 tactattgct tgaatataaa tcatatgctc acctctttat aaataactta gcaatataaa
    10501 gaaaaaaacg cacgaacaac ataggaatat aaacaaacgt ttttgtcaca acgtctaaga
    10561 tcgtgttaaa aaatagttat ctaaaaagca gaaagatact caaaataaaa aataaaaaaa
    10621 tgaataaaaa cgttttcaaa tacaaggtaa tttgttatta taaaataaag aaaaaacaaa
    10681 ataaaaataa agaaaagagg gaatgaaatg gtaaaagcag aagtaggaag atcttatata
    10741 tgcaaaccga tcggagtatc tcatgaacta atcggctgta ttgaacgtac atatatcaac
    10801 acggctgtcg tgtatgttga aagttatgac caacgagact atcaacttat caatgaaagc
    10861 aagtatcgca tgctcgtaaa attctgtgat attagcgcac ctactgaatt ggtttcttaa
    10921 acagtatttt tgagaattcg caagaactcc cttataatat aaataaagac agaagggagc
    10981 gagaagatat gccaaaaaga aaaagttcag ggaaaatggg aattattctt gcagtgatct
    11041 ctgcaattcc tgctatttta gctattgttc gtgcgatgaa ggaatcaaga gaaagttaag
    11101 cacacattat gagcgtcaaa caaaaaacgc atatacctgc ggtggcaggt tatgcgtttt
    11161 ttgttgtata aagacttttt tcattaacga tttttgatta gccgactccc aaaacaaaat
    11221 ttagcagttt atctgaataa gtgatttctt catctgtttg tttcacactt tcatttagta
    11281 gtcccatctc atcggttatg ataccatcca caccataaaa catcatgcga ctcatggcat
    11341 ctgaatcgtt gattgtccat acatatactt cttttccgtc ttggtgggca gaattaatga
    11401 agttacggtt gatcgtacta tattccattg tcagaaaatc agcaggcgtc acagggggcc
    11461 caacaatatt gaaaggtaag atgtagccca cataaaagtt tggttctgct tcttttaaat
    11521 ctgaaacggt ttggtacgtc aaactttgca aaatatgatg gttctctaag ataactttgc
    11581 ggtatttatg gacaaatcgt tcgacaaggt catccgaatc ttttttagag gtcttgatct
    11641 caataagtaa tttttggttt agcttatgtg ctttttccaa ataagaataa aaagaacaaa
    11701 caggggcttg cactccgttt tcagtaactg taagatttgt caattctttc aatgtaagat
    11761 cctgtggtgt tccatttacc cctgttaagt gacgaagatt tgcatcatgc atcactacga
    11821 aatgaccatc ttttgtttcc tgaacatcca tctctacata atcaggatga tagtctctgc
    11881 ttgttttttc aagtgcttct aaagtatttt gcgccccatt ttgggtactc acacctctgt
    11941 gagagacagt cactggtttt gtctgaacag ctgaagtcag ataatccata ttgtaaagac
    12001 agacgccaat accgaaaaaa actgcaaaaa gagtcaaacc agtgtttttc agcgcagaga
    12061 aacgcaagtt gggggcttgt gggataaacc acttagggat ctcaggcaag aagccttcgt
    12121 catccatgaa atcaacaatg atataaaaaa tccctacggt agacatcacg atattgaata
    12181 ataaaatccc ttgtaaaagc gtcatagcaa acacagcgct gatcaatgca tagtctggga
    12241 aaaattgttc aaccatactt tgtgccaaaa taacaacgat atatcctaaa gaagaaagga
    12301 gtaaaatcgt accgccaatc acaataaatt ggcccgtgat ggccaaaaga cgcgatttgg
    12361 tcaatgacca gctttcccga atggctgctc gaaaagaacg atctcgtaga atcatttccg
    12421 gtaaagcaaa aatcagtcga atcccaatat agccaaggaa aatataaacg agtaaaaagg
    12481 aactgacgat gatccaacga ttagcaaaaa taaaatccat aatgaaagcg gggattttta
    12541 tttttgacag tagatcagaa ttaaaactta atccgctgat cggtagaatc aataaaaaat
    12601 aagcaaggaa aaacaaaaaa gtaatgggtc tcactttctt cagctgaagt atcgtcaagt
    12661 gtaagagttg cttcaaagag attggttctt tctttttaat aaaaaaaaca ctcatcaaaa
    12721 gaaaagtaaa ttcgaaataa actaacagta aaatcgtcat caaaactacg attaacgaga
    12781 gtagaacccc aggatgttga gaaaaaattg aaggaatatt gtcataagac aaatagtcaa
    12841 ttgcccctcg gtgtaaaata aaacgggtcg tactgctcaa taagggtaaa caaataaata
    12901 aaataaagcc atgcatcagc aagacatcac ggaaataagc ttgtgttcct tttaaaaagt
    12961 cccaagcatt acctaagcta cgtttcaaat aattcatcct atcacctgct cttcacattg
    13021 tagcgcaaat ctaatgaaaa aggaaagggt gcagtgtgtt ttgcagaccc tttcccagat
    13081 ttaac
//
LOCUS       DAYYGS010000075        12736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000075 DAYYGS010000000
VERSION     DAYYGS010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.075"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA44_002139"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="ACHA44_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8905401.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="ACHA44_002140"
     CDS             complement(322..891)
                     /locus_tag="ACHA44_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFM8905402.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="ACHA44_002141"
     CDS             1077..1979
                     /locus_tag="ACHA44_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFM8905403.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA44_002142"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="ACHA44_002142"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFM8905404.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDDLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA44_002143"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="ACHA44_002143"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFM8905405.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA44_002144"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="ACHA44_002144"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFM8905406.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="ACHA44_002145"
     CDS             8547..8732
                     /locus_tag="ACHA44_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HFM8905407.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="ACHA44_002146"
     CDS             complement(8794..9990)
                     /locus_tag="ACHA44_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HFM8905408.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="ACHA44_002147"
     CDS             complement(10160..11596)
                     /locus_tag="ACHA44_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFM8905409.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="ACHA44_002148"
     CDS             11889..12698
                     /locus_tag="ACHA44_002148"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8905410.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2672 c         2250 g         4011 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaatcatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaat
//
LOCUS       DAYYGS010000076        12736 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000076 DAYYGS010000000
VERSION     DAYYGS010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.076"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            95..538
                     /locus_tag="ACHA44_002149"
     CDS             95..538
                     /locus_tag="ACHA44_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFM8905411.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(544..1728)
                     /locus_tag="ACHA44_002150"
     CDS             complement(544..1728)
                     /locus_tag="ACHA44_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HFM8905412.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            1996..2694
                     /locus_tag="ACHA44_002151"
     CDS             1996..2694
                     /locus_tag="ACHA44_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HFM8905413.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2701..3378
                     /gene="nth"
                     /locus_tag="ACHA44_002152"
                     /pseudo
     CDS             2701..3378
                     /gene="nth"
                     /locus_tag="ACHA44_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
     gene            complement(3504..4037)
                     /locus_tag="ACHA44_002153"
     CDS             complement(3504..4037)
                     /locus_tag="ACHA44_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905414.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4334..6715)
                     /locus_tag="ACHA44_002154"
     CDS             complement(4334..6715)
                     /locus_tag="ACHA44_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HFM8905415.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6705..7352)
                     /gene="recU"
                     /locus_tag="ACHA44_002155"
     CDS             complement(6705..7352)
                     /gene="recU"
                     /locus_tag="ACHA44_002155"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HFM8905416.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7416..7955
                     /locus_tag="ACHA44_002156"
     CDS             7416..7955
                     /locus_tag="ACHA44_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HFM8905417.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8045..8464
                     /gene="gpsB"
                     /locus_tag="ACHA44_002157"
     CDS             8045..8464
                     /gene="gpsB"
                     /locus_tag="ACHA44_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HFM8905418.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8486..8853
                     /gene="rnpB"
                     /locus_tag="ACHA44_002158"
     ncRNA           8486..8853
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHA44_002158"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9062..10228
                     /locus_tag="ACHA44_002159"
     CDS             9062..10228
                     /locus_tag="ACHA44_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFM8905419.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10274..11770
                     /locus_tag="ACHA44_002160"
     CDS             10274..11770
                     /locus_tag="ACHA44_002160"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HFM8905420.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11851..12534
                     /locus_tag="ACHA44_002161"
     CDS             11851..12534
                     /locus_tag="ACHA44_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8905421.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            12543..>12736
                     /locus_tag="ACHA44_002162"
     CDS             12543..>12736
                     /locus_tag="ACHA44_002162"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905422.1"
                     /translation="MTEVVSSKIFRIIFRKSYFWNTVYQEVVSLPFSSESQGTISKNS
                     FSCLMNIVTCDSELAGEHRL"
BASE COUNT         4272 a         2229 c         2630 g         3605 t
ORIGIN      
        1 ttggtttcag attgggtttt cctacaaaat atttttatct ttgttgaata ggaaacaaaa
       61 aaacaatata atgaaagaaa aagaaggtgt agaaatggat aaaagacaac tgctaataga
      121 agaatatctg gatttagcta atgagattat tcatcgtaat aaaccagaaa tggaaaaaga
      181 atttgcagga tttactctaa atgaactaga agtgattgaa catatcggga aaatccctga
      241 tcctaacgtg acaaaattag cgaatgctag ttacatgact agaggagcca tcagtaagct
      301 aacaaaaaaa ctgatagcta gagggatcat aaacacttat caaagcgaag agaataaaaa
      361 agaaatctat tttcaattga cgccaatcgg acaaaaaata aggaatagac acgagcaact
      421 ccatcaaaaa tgggtacagc gagatgctac tgtatttgaa aatatgtcaa aagaagaatt
      481 tgacacagta tttcgtttta tcggtcgata cagagaattt atacgaagta gcaaatgaag
      541 ctactatttt ttattgtagt gataataggc aaaaatcata agcaaaaatc cgaataccgt
      601 cggaagaaat ggtgctgcag cataaagctg tcctgcgatt agcgggccga tgacgcgcga
      661 tagagattgt atactttggg taccgcccat tacaagacct tgctggttat ctgagacaga
      721 attggatagt tggccattga atacaggagt aaaaatagaa tcaccgaaac cataacagat
      781 aatcccaaat aaaaacaaaa tcggcagtag aagaattcca gataatgtga tgaatagata
      841 ggcgaggatt tcactgaaca tccctattct tgtgatctgc tgttctgaaa attttttgag
      901 caatcgaggc ataatgaaca attgcgagac agtatccaag attccaatca aagagaagct
      961 taaaccaacc aacccagcct tccactgaaa ggcgtcaatc gaaaattgag aaaagataga
     1021 ttgaagagag ccagctgcca accaaacagc aaaacctgct gtcaataaag gaataacgga
     1081 tgaaatcttg aataattgtt gtaattggtg aaaaggtctt acatgggaga aacttaaatc
     1141 tactgaacgt ttttttattg gaaggctttc tagcataaat gtgtagccat aaacggcatt
     1201 taagaaagta aagagtgctc caataaatat aggaacagaa ttccccaact cagctaatag
     1261 accccctata ataggaccca gcgttgttcc tataccgact aaagcaccca tccatccaaa
     1321 aactttggtt ctttcattag gttccgtgat gtcggcaaag taagcatata aggtggagat
     1381 ttctcctgcc gtcaatcctt caatgatacg accaaggaac aacatccata tcgaatttcc
     1441 taaaccaaaa agcaaatatc cgatacttga tcctaataaa ctgataataa ggatcggatg
     1501 acgcccaaaa tgatcactta agctaccgag aatgggggca gataagaagg cagctagagc
     1561 ataaacagac atgagcaaag tgatatagaa agcttgctga tgtatggagt gagaataagg
     1621 cagcgttaaa aacggaagta caggactgac gatagtaaat cctaatccag tgaataagac
     1681 agagacgtat ccaaaaactt tagaatgttt atttgtgaaa ctgtacatgt aaaaactcct
     1741 ttatgcaata atgttaactt ggaaacaaaa atagtgtata gtaattttgt ttccttgtca
     1801 acattctaag gaacagcgaa atctacttaa tttatttatt ccgaaaagtg aagatacgca
     1861 tcaaaaacat gaacaaacaa aaatcaagta gacgacattg taaatctgac cgatcagtga
     1921 agtaaataga aaagaaacct gttcttgctt cccatgtttt ttttacgtat actgtgattg
     1981 agcggaggag aaatcatgtt gaatttagaa aattacttaa gcgctggaca aacagtcata
     2041 tctaatcttt tacttaaaaa ttatcatagg atcggtctac aatctgaaga atttttattt
     2101 gttctacagt tgcatatggc acagcttgaa ggagatactt tccctgattt gcagatgatt
     2161 gctatggata tgggaatcaa acaagatcag atttttcaaa tactggatcg tctcgtaaca
     2221 aatggattta tcaagataga aacgacgatt gtcaacggta aaaaagcaga tcgatataat
     2281 ctttacccca tctatgatct attaggtgag tatttaaaaa cacaagaaaa aaaatacgaa
     2341 gaaaaaacac aagaaaaaga aatccagtca gtgtatcaat tatttgaaca agaatttgga
     2401 cgcccgcttt cttcgatcga gttccagcgg atcggacaat ggctagaaga agaccattat
     2461 cagccggaaa ttttgaagct agcactgcgg gaagccgtat taaatcaggc ttatagtttt
     2521 aattacgttg accgcatttt gctatcgtgg gaaagaaaaa atctacgtac aaaacaacaa
     2581 gtggaagaag accaaaaacg aagaaaacag cagcttcttc aaagagaagt agaacagtca
     2641 gctcaacaag aagaattgcc gaaagtctca ctgaaaaatt ggttggagga gtagcggaat
     2701 atgttaagca aacaaaaaac aatggaagct ctagaaacga tgtatggaat gtttcccgaa
     2761 gcacatggag aactcaagca caataatcca tttgagttat tgattgcagt gatcttaagt
     2821 gcccaggcga cagatgtatc agtgaataaa gcaacgccag atttgtttgc ctcttttccg
     2881 acacccgatg cgttagcaga agcttcaatc gatgaaatca ttcttaaaat aaaaacgatt
     2941 ggtttgtatc gaaataaagc aaaaaacatc aaagcgtgtg cacagcagct gattgaacgt
     3001 ttcgatgggc aagtgccaac gtcaagagaa gaattgatgt ctcttcctgg tgtaggcaga
     3061 aagacagcaa atgtcgtatt gggggatgca ttcgggatac cagcaatcgc agtagatacc
     3121 cacgtggaac gtgtctcaaa acgcctgagg atctgcaaac tagatgctac ggtcatggaa
     3181 gtagaagaaa cactgatgcg gaaagtacca caagaattat gggtaaagac acatcataca
     3241 ctgatttttt ttggttgata tcattgcacg gcaagaaatc ccaagtgcga ggtatgtcct
     3301 ttgctatcaa tatgtcaaga tgggaaaaat cggatgcgtc taaaagaaaa ggcgctaaaa
     3361 aagaaaccac gcctgtagac gtggtttctt taattgcaaa taaatgtatg aattatcttg
     3421 ttattatgga gaaaagctgt ttcgtttatc ttttttgcat gaaaaagtaa atgtacatac
     3481 tcagtagcta ggatggtaac ttattacaat ttattgcgat aaaaataaaa gttgttattt
     3541 ttattgggaa aatacccgat gtagaaaata atgccttcca atatacctat aaaataagtc
     3601 cagtattcca aaggattata taaataagtg acgtatctat ctaaaggtgt aataaaaatg
     3661 ataagtgata ataaaaaaat accatttata actaaacata aactaatgga tttgggcggt
     3721 ttcaggctca tcagaaaaat atagattctg gtgaacaaca accataaaaa agtgagaatc
     3781 attgcatgag agcttctttc cataaatttt cctttaagag tagtgcttaa aagtacagaa
     3841 aaaataataa atgcaaggat aggagcaatt aaagtaaatt tatttaataa taaacaaaat
     3901 ttttcagctt ttttattctt ggaataacga ggtattgttt tcgataaaaa aagatggtgc
     3961 agacaaatta tgataattaa gcatactatc agataactca aaatgaagcc ttctttcaaa
     4021 aaattattca aaaccatata ttgtattttt tatttgataa taacaatggg attattaaga
     4081 agtggcacta aataaaagac tagtgtgtat ttagccgatt taattataaa caagtaaatg
     4141 gataaagtat atgtaatgat aaaagaaaaa tggattttct aatttttaag ctaaatgtta
     4201 gatcacacat gtatatgctg gatagtctta taaaaaatca tgtaatacag cttggtacat
     4261 gatttttatt tgtagctgtt ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt
     4321 ggtttctttt ttactatctg tttccagaag ctgaggtgct gctggatggt gtatggtcat
     4381 ttgcagcatc cgctccgcta gttgctggct cactggaaga agcaggagga gcagatgaag
     4441 acggtgtcga gctttcttcg gatgaagaag aaggaaccgt tgttgaagac gctggctcgc
     4501 ttgtttctgc tgtcgtgctt tcttttgagg actcagcagt tgattcgctt tgactagtag
     4561 agctagagct ttgagtcgtt gtctctgttg ttgagcttcc aggagtctgg acatagctgg
     4621 aagatggaat agttgttgat ggtgtaattg cattcgatct agctgtatat tgatctttgt
     4681 agtacagttc accgcccaca cgaattaatc cgcttggcat ctcccagtct gtatttgtca
     4741 cgttagctga aacatactgc ataagctcgc gataaacatc agaggctacg tgtgacgatt
     4801 ctgaagtgac aggcgtcatt tttttattgt aacctgtcca cacggaaatc gaataatttg
     4861 gcgtatagcc cgcaaatagg atatctggat acaccccaga agaaatacca agttttgcat
     4921 actcgtcatc tgtgtaattg gatgttccgg tcttaccggc ttgatataac ccagcgattt
     4981 gcgcattcgt accagttcct tcagtgatcg tatcttttaa aatgtctgtg atcatataag
     5041 ctgtttctgg agacatggct gtttttccat ctggctcata agtttcttct gttccgtctt
     5101 ggaagacgat tttattaaca tattgaggtt tgtaataagt ccctccattg gcgaaagccg
     5161 cataggctgc tgccattttt aaggaagaag ctccgtattt tgtcccatct tgttcttccg
     5221 tattactaga gatcgcattc gattgatgga tcgtactgta ttcgatccca agatttttta
     5281 agaaagaagc cactttatct gaaccgactt cattgaataa tttgactgct ggaacattac
     5341 gggataagta aagtgcttgg cgtaaagtaa tggtgcccat atattgatta tcccagtttc
     5401 ctacaggagt cgaagtccct tcatagttat acggtgcgtc agtaatagtt tttccagtag
     5461 aatatttcaa atattcaaaa gctggtccgt aatctgtgac aggtttcatc gtagaaccaa
     5521 aatcacggga agtattcact gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac
     5581 gtccgccaat ctgggctttt actttcccgg tattggtgtc gataagggtg gaggcaactt
     5641 gcatctcgtc gtctgggtaa gagacatact gatccgtgtt gacgatatca tatagtttct
     5701 tttgggcgtc cagatccaaa ttcgtataaa tctctaaacc gtctgtgtag acattcttat
     5761 ctgttttttc ttgtacttca ttgatgactt ctttgacata attatcgacg atcttagtat
     5821 tgtcatcgga ttgagttagt tcttgcagac catccgttac cggtacatta acagcttgat
     5881 catattctgt ttgagagatt ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc
     5941 ttttcttcgc ctgatctgga taaacataag gatcataggc ggaaggggct tgaggcatcc
     6001 cagcaagcaa tgctgtttgc ggtaaagaaa gttctgataa tttcttacca aagtacatct
     6061 cagaagctgt ctccatcccg tacaagccgt ttgacatata cactttattt acgtagtagg
     6121 tcaagatctc ttgtttcgat tttttttgtt ccaatcttac agccatccac gcttcttggg
     6181 ccttgcgttt cagagtctga tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt
     6241 gctgtgtcaa agtacttccg ccttgcagtc cgccagaggt aaagttagag agggcagaac
     6301 cgatgatccg aatagggtca accccgatgt gtttataaaa acggcgatct tctaccgaca
     6361 cgatcgcatc ttctaatgtt ttcggtaatt cattcgcaga aattttttca cgcttttctg
     6421 cgcctaaatc ttcgaatagt tcgccatctt gtgtatatag tttggaagat acggtagcgt
     6481 ctaatttttt atccgtcaat tcgggcgcat cttttgcgta ataccagaaa agtcctacac
     6541 ctgctaaaaa gaggatacag aaaaatgaga gtattcctaa aaggatcttg ataaataatc
     6601 cttttttatg tgttccagag gactttccag aacctttgcc agaattcttc ttcacactct
     6661 ttttaggagt aggctgtttc tgtcgtctag agcttctagt ttgttcattt gccattcttg
     6721 ctttctcctt caatagataa tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc
     6781 cgcggagaaa taccagtttt gatctctatt cctagttctt cgatcaaaga taaaggtagt
     6841 gatttgcgtc ctttttcctt ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa
     6901 taaaaacagc gcttcaaaga cgaaaaccat aacaagacaa agcagacccc attttgttcg
     6961 aggcatgctt tcatatgttc gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt
     7021 ttgttcttgg tttcttttgc ttcaaagtcc aagtaaaagc ctcgatagac gccattatag
     7081 tctgtagttg aggcttgggt aaagtaggct tccttgatca cagcagcact tctcttagga
     7141 tagtctactt tcacgatttg gataggggta ggctttttat ggataaccgc caacccgcgg
     7201 gaaagataat atttattact ttcattgatt gcttcttcaa agcgcatccc ccgattgccg
     7261 aattcagtga tctgatttct tttttcttga attttttcga cgggagaggc attcttataa
     7321 taaggttggc catttggata gcgcaatacc atctttaatc acactcctag gccacattat
     7381 agcaaaaaag cggatagaaa gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg
     7441 tgtctgggta tcgaagtttc gaactcggtg tttttaaaga aaatgatcct aaaatccaag
     7501 ttgttaaaaa tgttttaaaa aaagaactcc atcagttagc agaagaaggg ctggaatggg
     7561 tacttgtcag tgggaatttg ggcgtcgaac tatgggctgt gcaaatagta gcggaattga
     7621 aagaagaata tccggaacta tctcttggta ttttgtatcc tttttctgat tttggtaata
     7681 actggaatga agggaaccaa gaaaaaaaac aattagcaga acaattagca gattatgtcg
     7741 aagctgttag ccatcaaccc taccaatcac ctagtcagct aaagaatcac actcgtttta
     7801 tacttgagca tacagtgggc tgtctgctga tctatgatga agaattccct ggaaaaacga
     7861 agtatttttt tgaagatgca cagaattacc aagcagatca tccctacgaa attcggctga
     7921 tcagtatgga cgatcttcaa aattttagtg aataatcgat attgtgtttg atttatcaac
     7981 ttttttccta tacaatgaaa aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt
     8041 aaatatggcg aatttggttt atagtcctaa agacatttta caacaagaat tcaaaacaaa
     8101 aatgcgcggc tatgatcccg ttgaagttga tgaattctta gacaatatca tcaaagatta
     8161 tgaaacttac agcaaagaac tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa
     8221 agtagctcaa ttatctaaaa ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc
     8281 accaaagagc gcagcagtga ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg
     8341 tgaagtattt ggtaaaaagc ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa
     8401 tccaaataac tacacaaatg cagacacaag cttagatgat aatgaaaaaa cacgtcaatt
     8461 ttaatttttg attaatgaat ttgcggtttt cgggtaatcg cggtctgact tgatcaggct
     8521 gaggaaagtc catgctcgca caggctgtga tgcctgtagt gttcgtgctt agcgaaacca
     8581 taagctaagg tactctttgg agtaacggca ggaaaacaag ctaaggcttc ggctatgctt
     8641 aagtatcctt gaaagtgcca cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc
     8701 ggtaaacccc tcgagcgagc aacccaaaca atggtagggg cgctttttgt ctggaagaag
     8761 aacaggacaa aaaggcagta taactgcaga tagatggtta ccgctttgtt ttttctccct
     8821 ggaaaagcaa agtacagaac atggcttata gaaaaccgca aacatcaggg aattaggtcg
     8881 tgacaaaagt aatgcgttat aagaaacaag aaaaacattt gaaagctatt tttcaatact
     8941 tcagatgaat cgttcttatt acaaaacaaa cttttgtatc accggttatt cggaatttga
     9001 gtaaaagtgt ggggtaggac tgatgtccca cctccttttt ttataaataa ggagattgtg
     9061 tatggaaaaa aacaggaagt tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact
     9121 ggttggtaaa gaattaagag atcttggaat cgaatgccaa gttgagaacg gacgtgcacg
     9181 ttttgaagga acaatggaaa cgatcgctac agctaattta tggttacgta cagcagatcg
     9241 agtaaaaatc gtggttgggg aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt
     9301 aaaagcattg ccttgggaag attttcttcc actagatgca gcttttcctg tagctggaaa
     9361 atcgatcaag tcgaaactat atagcacacc tgattgccaa gcgatcacaa aaaaagcaat
     9421 cgttgaacgt ctccgtacct attatcaccg tccagcttct gtaccactga cggaaactgg
     9481 cgcacatttt cagttggaag tagctttgct gaaagaccat gtgatggtaa ctctggatac
     9541 gacaggacca agtttattca aacgtggtta tcgcttagaa aaaggtggag caccattgaa
     9601 agaaaacatg gcagcagcac ttattatgtt gacaaattgg cgaaaagacc gtccatttta
     9661 tgatcctgta tgtggttcag gtacgctttg tattgaagca gccttaatag gccataatat
     9721 cgcaccagga ttcaatcgtg agtttgcttg cgaatcttgg gattggttca gtcaagaaat
     9781 catggaaaat gttcgatcag cagccgaaga aaaagcagat tatgatattg aattagatat
     9841 tactggttca gatatcaatg gacggatgat tgaaattgcc aaagccaatg cagaagaaat
     9901 cggattaggc tcttcgatta cctttaagca acaagcagtc aaagacttca aaacggataa
     9961 agaatacggg gtcatcgtgg ctaatccgcc ttacggagaa cgtctaggag aagaagaaac
    10021 tgttcggaaa ttatataaag aaatgggcga tgtattccgt ccattaaaaa catggagtaa
    10081 atatatcttg actagcgact tagcgtttga ggaattttac ggacaaaaag caaccaaaaa
    10141 aagaaaattg tacaatggtg cattgcgtac ggatctattc cagtattggg gaactcgtcc
    10201 gcctagaaaa aaagagaaag acgaataatc ttactaattg attcattcaa ttggatgttt
    10261 ggaggaatag ggaatgaaag aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct
    10321 acaacaagct ttaggaatcc tcgattggga tacacaaaca ggtatgcctg aaaaagctag
    10381 tgcataccga agtgaagtag acagctatct ttacagcatt tatttttcaa aaaaaatcgg
    10441 tccaaaaatc aaagaagcca ttcgctattt ttctgaacat ccggaagaat tgtcggaagt
    10501 tggaactgcc gtttttgcca aagtaaaaga agagtatgat ttggaacatc gcgtaccgga
    10561 agatcttatg agaaaatata cagctgccgt ttcttcagca cacggacaat ggcaaaaagc
    10621 tagagaaaca caacaatttt ctgattttca acacgcattg aatcaaaata ttgaattaac
    10681 aaaacagttg ataccttact ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa
    10741 tcaatacgaa cccgggatga ctgtcgaaat ccttgataag gtatttgatc agcttaaaga
    10801 aggaattctt tcgatccgcc gtacgctcca agaaaaaggg actgaacctg aaacagactt
    10861 tttaaatcgg agaatgacga aaaaacaaca gaagagattt gtagtgaaag taatcgaaca
    10921 acttggttat gattttagtc gtggtcgttt agatgacact gtccacccat ttatgactgg
    10981 aattaatccg aatgatgcac gaatcacgac acgatggaac gaaacagatt tcaaaatggc
    11041 tgtttttgga gtgatccacg aggctggtca tggtatgtat gaacaagaca tcgatgataa
    11101 atatgcgtat acaccggttt atgaaggtac atcaatgggt atccatgaat cacaatcgtt
    11161 gtttaacgag atcattattg gaagtagtcg cagcttctgg aaaaagcaat atcctttctt
    11221 tcaagaatgt gcagaaggaa cattcgatga tattcctttt gacaaatttt atcgttcatt
    11281 gaaccataca aaatcaagtt tggttcgtat cgaggcagac agtttgactt accctctcca
    11341 tatcatcatt cgttatgaaa tcgaaaaaat gattttcaat gaaggggtag atgttgctga
    11401 actacctgaa atatggaatc aaaaatatga agagtatctg ggaatacgac caacaaatga
    11461 tgtggaaggc atcctacaag acgttcattg gtcaggtgca agctttggtt attttccctc
    11521 ttatgcatta gggtacatgt atgcggcaca attacttcat tctttgcagc aagaaataga
    11581 tgtggaaaat gttctagcgt cagacgatta ttctccaatc aaaaactgga tgtcagagca
    11641 tatccatcaa tatggtgcat ctagaaaacc aaatcaatta atcatggatg caacacatga
    11701 accgcttaat ccgcaatacc tgatcgacta tatgaaagac ctgtatttta atgtatatca
    11761 ggtaaaataa aatacaattt acaaagatag ctgaaattca tctaatctat agatgttttc
    11821 agctatttac ttaagaaaga agtggaacga atggataaat tacaagtgat cgctgattat
    11881 agtatggacc aattagcgca tgatcaaaca ggacatggga gcgatcatac caaaagagtt
    11941 gtcaagttag ctgaaaaaat tttggagacg gaaccgcagg cagaccgctt tgtgacgctt
    12001 gcagcagctt acctacatga tacgattgat gataaagtcg ttaaaaatga gaacgaggca
    12061 aagcaacagc ttcgtgtatt ccttcgctct ttgccaatag tagaggaaca gatctcgatg
    12121 attttttcca ttatcgaaaa tatgtccttt tctaaaaatc tctctgaagc ggccgaacta
    12181 tctctggaag gtaagatcgt tcaagacgct gatcgtatcg aagcattagg agcaatcggc
    12241 attttgcgta ctgcttattt cggcggtgga catgggcacc cgatttttga tcctgaactg
    12301 tatccgcaaa cattcaagga caaagaaaat taccgaaaag gcacgacagt gatcaatcat
    12361 ttttatgaaa aactgttctt gctgtctgat aaaatgaata ctgattacgg atatgaagaa
    12421 gccaaacgaa gagaaacctt catgcgtgaa ttcttagaag aattttttct ggaatggtct
    12481 attgacgatg aaaattttac tccagacaat tggatcaaaa ataacaaaaa atgatggagg
    12541 ttgtgacaga agtggtcagc tccaagatat ttcggatcat tttccgtaaa agttacttct
    12601 ggaacactgt ttaccaagag gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa
    12661 tttcaaaaaa tagcttctcg tgtttgatga atattgtaac ttgtgattcg gaattggcag
    12721 gagaacaccg tttatt
//
LOCUS       DAYYGS010000077        12034 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000077 DAYYGS010000000
VERSION     DAYYGS010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.077"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(340..921)
                     /locus_tag="ACHA44_002163"
     CDS             complement(340..921)
                     /locus_tag="ACHA44_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8905423.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(1104..1730)
                     /locus_tag="ACHA44_002164"
     CDS             complement(1104..1730)
                     /locus_tag="ACHA44_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFM8905424.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1752..3083)
                     /locus_tag="ACHA44_002165"
     CDS             complement(1752..3083)
                     /locus_tag="ACHA44_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HFM8905425.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(3238..3639)
                     /locus_tag="ACHA44_002166"
     CDS             complement(3238..3639)
                     /locus_tag="ACHA44_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HFM8905426.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3725..3931)
                     /locus_tag="ACHA44_002167"
     CDS             complement(3725..3931)
                     /locus_tag="ACHA44_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905427.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(4026..5528)
                     /locus_tag="ACHA44_002168"
     CDS             complement(4026..5528)
                     /locus_tag="ACHA44_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFM8905428.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5546..6001)
                     /gene="argR"
                     /locus_tag="ACHA44_002169"
     CDS             complement(5546..6001)
                     /gene="argR"
                     /locus_tag="ACHA44_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFM8905429.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(6099..6716)
                     /locus_tag="ACHA44_002170"
     CDS             complement(6099..6716)
                     /locus_tag="ACHA44_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFM8905430.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6937..8310
                     /locus_tag="ACHA44_002171"
     CDS             6937..8310
                     /locus_tag="ACHA44_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8905431.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8385..9497
                     /locus_tag="ACHA44_002172"
     CDS             8385..9497
                     /locus_tag="ACHA44_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8905432.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9569..11206)
                     /locus_tag="ACHA44_002173"
     CDS             complement(9569..11206)
                     /locus_tag="ACHA44_002173"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFM8905433.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11374..11982)
                     /locus_tag="ACHA44_002174"
     CDS             complement(11374..11982)
                     /locus_tag="ACHA44_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8905434.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3177 a         2544 c         2172 g         4141 t
ORIGIN      
        1 aataagccca accaaacgaa aaatatgagc agctattttt atttggctgt tcttattact
       61 tgatgcaggg cagctttttc acaacctctc cactaaacgg tgttcaatca gctaaccttc
      121 ggtattagct caaaaaacgg caaaatatga ggaactattt tgccgtttgt ttctctaata
      181 ctcaggtcaa agcgctgatt tcacaacctc taatacacgg tgttccagaa gtagcttctt
      241 cgaaaaataa gccgaaattt cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta
      301 tttcttgaag ctgaccactt ctgtcacaac ctctaattat taaagaacct tttgcgaagt
      361 taacaatctt tctttgatca attgaaaatt ctgcctaagt agtgatagat cctgtgtgac
      421 tttcaacaat aagatacttc cctggatttc agcaatcaac gctttggctt gacggtctgc
      481 ttcttcatcg gacagttttt cttcagaaag tgccaggata ttttgtttcg tatacttgac
      541 ccattgattg actaattcta gtagcaacag acggtattct tcatccactg tactcaactc
      601 aataatcaaa ttacctacag gacagccatg ataagtgatc tgtgtctcat gatcagcgaa
      661 aatccagtcc aacatttctt ctaatgcttc ttctggctgt tttccagcat ttaagatgtc
      721 ttcgaacaat tctgtctgcc atacgtccat atgcgcatgg atcaccgcta gacaaacttc
      781 tttttttgaa gaaaagtaat aataaaattg accttttcct gtatcagaag ctgctagaat
      841 atctcggatt gacgtttcat gatagccttt tttataaata agatctttgg tcgcttccaa
      901 gatcttttcc tttgttttca atgatattct cctttttcat ttctcatagt cctatattat
      961 acagtaaatt catcattttt tcttcttaat cgtttaaaaa agactgtgcc aaattatctg
     1021 acacagccct cactataaaa ctgttctcat atagctagat gttcattgat tcaagaactg
     1081 cgaggacaaa agcaattcct atcttatgat agttgctcta atacttcctc tgttgtcatc
     1141 acttgatctg caataaagcc aaagttcgtc aacgctgcat ccattgcttc ttggccagga
     1201 gcaccaattg catctgtcgc taccacaaca ttgaatccac tctctaccaa ttcacgcata
     1261 tgggaatcgc tacacaagtt tccgttcatt ccaccaagaa ttactgtgtc gattccattt
     1321 tttctcaatt ggagcgccaa atcattgctt tctggtccaa agattttatg aggagagctg
     1381 atgatcgtat tttcatccaa ataaggtttc aattcttcga taaaatcggc tcctgaacct
     1441 tcttccactg attcatattg actttttcta gcaaacatct gctgctcaag catcatatgt
     1501 tctcctgcac cgccaaattg ccatttatgg tcatgtgggt aaaaataatg aggagaaata
     1561 aagagtttgt atcctttttc ttttgcttgt tttaccattt caatcatatt ttcgatcgta
     1621 ttgtattttt ctaagatatc ttttgtcaaa ccaaagccac cgcctgctgg tttcaagaac
     1681 tcgacttgcg ggtcagtgag aactactgct gtatttttat tattgaacat ggtggattct
     1741 ccattcgtta tttattttaa tacatcattt agagcttcac tcattgtcgg atgagaatag
     1801 atttgatctc tcaataacgt ataaggaagt tttccattca tagctagcga aatcaagttg
     1861 atgacttcat gagaatcttc cgcatagata cttgccccta gaatcaagtc agtttcaggg
     1921 tcgactaaaa ctttgaatac acctttagga tcttctaata cttgtgcttt agggacaccg
     1981 cttgtcatat acttgaataa tttgtaattg acgccttgtg ctttggcttg tttttctgtt
     2041 aaacctacat tggataaagt cggagtgata aatacactgt aaggcacagc tttccggtta
     2101 ttcgttgttc gtttattctc gcccttcaac tgatccataa tgattcgata atcatctaga
     2161 gagatataag tgaactgcaa tccgcctttg acgtctccaa tcgcccaaat gttgtctgca
     2221 tctgtacgaa ggtattcatc cacagcgatt gctccgcgat ctgttagttt aacatctgta
     2281 ttttctaatc caagtgatgc tgtacttggt tttcttccag ttgcagccaa aacttttgac
     2341 gcttggattg ttacttcttg gccatcttct gtaaaggtga cggcagctgt tgattcttgg
     2401 tctgctactt gatctacaga tacacctaga tggaattcga ttcccgcatt tgtcatgtct
     2461 tctaaaatca tttgcgcgat atcttcatct tcacgtggaa gaaattcaga atgggcatca
     2521 agaacgacaa ctttagatcc gtattcattg aacattgatg caaattccaa gccaatatat
     2581 ccagccccga tgatcactaa tttttccggt aattgttcct gatccatcgc ttgcgtagaa
     2641 tcaatcaagt agcgacttgt attcaatcct ttgattggta aaatgattgg ttcagaacca
     2701 gtattgatga aaatccgttc gccttcaaca atcacttttt caccattatt cgtcacttcg
     2761 atttgatggt tagaaagaaa acgtgctgta ccgtccatca ctgtcccagt tgcctcatcg
     2821 ctgatcatat gataattttt ggcgcgaagc atgcccgtca atttttcttt ccgagcaaca
     2881 gcctctgtaa agtcgattcc tctttctcca ttgaaaatca atgatttaga aggaatacag
     2941 ccgatgttga tacatgttcc gccatacata cgtgttgact tttcaacgac taaaaccgat
     3001 tcaccttttg aagctaacgc cttcgctaaa gtcttaccgc cctttccaaa accgatcaca
     3061 atattttgat atttttccat catttttccc tcgcttcttt tttacctacc gattggtaca
     3121 aaaatatgat aaccgataat tgtctattct ccaaatcatc tgctttattt gtcaaaaaaa
     3181 accagacaaa aagcggtcct ttcacgtttt tagtctggtt cgatttcctt tattcttcta
     3241 aacaatcgtt acttctacat cgatatttcc tctagttgct ttggagtaag ggcagaattc
     3301 atgcgcatct tctgcaattt tttgggcttc ttcttcgctg atgccatcga tatgcacttc
     3361 taatactaca ccgatctgat agccgccatc atctaaatta taaagagaga cacgtgcttt
     3421 tactgtggac tctgctgtca ttcctgcttc tttgatcata tgttgtaccg ccccattgaa
     3481 acaagaagca taacctgctg caaataattg ttctggattt gttccttctg catggattcc
     3541 tggaggagtg attttcatat gcatcgagcc atttggtgcc tcgacttctc cattgcgccc
     3601 gccatggttg atcattgctg tttcgtatgc ttttttcatt gtactgctcc tttcgattga
     3661 ccatttgcta cttggctata attatagtgt atgacaagta aggcgctttc tacaaaaatt
     3721 ctgcttattc gaataccaga atgccgtctt ctactatttt tccaaaaaac aaaaaaatac
     3781 tactcgtgaa caacagcgtc atatttgttc caaaataaat agcaatcttt tctacataat
     3841 cttttatatg attgatttta agatacatat ataccagaaa gttgacccca taaaccaata
     3901 ctgttaaaaa aataaccata ttcgttacca ttttttcctc cttttgctgg tctccctacc
     3961 agcactcgat ttaaccatac cacttttttt gccgtccaag caactagcct ttcaaaaaga
     4021 ctggtttatt ctgctgttaa tgattcaaga tcagaaaaat gtccgaataa ttttgctaat
     4081 tttactaacg ccaacttgtt ctttccgaat tcatcatttt ctatctgtaa tactagtaac
     4141 ggctcatgta agcttaaacg gatcaaaaac cagccgtttc cgtatggctc ttctagatca
     4201 tatcgaatac cttcttcatt ttccgggtcg aagttcatcc ctttttcttc ttccaacagt
     4261 cgcttcattt cttctaatat cttgtttcct tgttcgcgtg gttcatcgct gaacaatttt
     4321 aatcggattt cttgtacttc tacgggctgt ttcaactctt tgatttgttc atctaatgtg
     4381 ataccttttc tttttaattc tggcaaaagc atcaggatct ttgcgatgac atatgcacca
     4441 tcatccagaa aatagttttc cttgaatgca gcgtgtccgc ttgtttcgat ggctagctgt
     4501 gtatctttcc caagacggtt aagttctaat gctttattga ttacatttcg atagccggaa
     4561 atataacgaa cttggatgcc tcctttttct tcaatgaatc tttgtagatg agaagaagta
     4621 ggggaattcg tcacgattct tgttcctgga tgctctttta atacgattgt acttaatact
     4681 gcgatcaaat tatttcgatt gatcactgtt ccagaagcac tgacaactgc agaacgatcc
     4741 acatctgtat cgaaaatgat cccaagatct gcttcatttg ctaaaacagc ctgcttgata
     4801 ctttccatcg cttctttatt atcaggattt gggatatgat taggaaatcg gccatctggt
     4861 tccaaaaatt gggaacctgt tgaatccgca cctaagctat tcaatacttt ttcagcaaag
     4921 aatccaccag caccatttcc ggcatcaaca atgattttcc aaccatttag tggtttgctt
     4981 tctagtgttc caatcccctt tttaattttt gtgactaaat cttgcgcata aggtgtcaaa
     5041 aggtctgctt tttctacgct tccagtttgt tcgcttttta ttttttgctg ctgtgatagg
     5101 ataaaagcaa tatcttcttt ttctgctcct ccttctgagg taaagaattt tattccatta
     5161 taataatagg gaagatggct agcagtcagc ataaccgcag catcgcacga gaactgttca
     5221 aattgagtgg acataaacat agcaggcgta gtagctaatt cgaaatcgat gacatgtatc
     5281 ccttgttctt tcagactttg gatcaacgcc tctttcagtg ctggtccgct tatcctactg
     5341 tcatgaccaa tcccaatcgt ccatttttta tttggatcgg ttgatttctt ctttttcttt
     5401 atccagtttg ctagtcccca gccgatcttt tttaccatgt ctgtcgtcaa gtttacttca
     5461 tatccttcaa ctggtaatgc gattcctcga atatccgatc cattttgcag cgctttcata
     5521 tctttcatat aaaagtcctc ctcatttatt ccctcatttt ttccagcttt tctgcaaatc
     5581 gctgtgcttc ttcttctgag cggcaaataa taatgaatgt atctacaccg gccatcgttc
     5641 cgacaacttc agggtaagcc acttcatcta gccaattcgt taccacatcg gcaccatttc
     5701 gttcagtcaa tacgataacc atgaactgaa cagactggat acgcaaaacc tcgcgtctaa
     5761 ttgctgtacg caaacgttct tcatttaaag aaacagatgg ctggttaaat aatgtatagc
     5821 gtactatttt gttttcattt tgacttttta ctaacttcat ttctcgaatg tctcgagaaa
     5881 tcgttgcttg agtcacatcg gcgcctgctt catgcagccg cgcaagcagc tcttcctgtg
     5941 tcccaatagg ttgttctgta atgatctggc ggatcagtgc ttgtcgctgt ttctttttca
     6001 tattagacac ctctcaatgt tgattatagc aaaagataag taatatttct tcatattttg
     6061 gtatttttta cttttatttt ttgttttcat tcaggttgct aacctttttc tattttaaca
     6121 cgcaaaatcg tcttcatttt ttcaggtgct gattttcttg gatcgctgat gtaaatctct
     6181 ttgtgttctt tagaagctct ttcatagcct tcttttgcga tgaatgcttc taatttctca
     6241 aacgttgctg gttcttcggc atagggcccg acgtgtaaga tttgggcaca aagaccttct
     6301 tccatctttt tgaatactac ttgttctttc aagtcttccg gaagtttgct agaacgctga
     6361 atggcttctt gtgctatcgc ttctgtaaca aagtcaggct gtcggatcat caattgataa
     6421 gaaaaatgtt cttttttcat cactggttca tgcaaatact tttcttgcag tccccattgt
     6481 ccttccagtg gatagacagt atacatctga taatcaggaa tcagccaatt ttttttaggc
     6541 gacatgcgta ttgcatagct gactgcatat agacaggcta ctcttcgttt aaagtcttcc
     6601 tgattcgggt cgccgatccc tctgatgcta aagaatgatt ttttaggaac ctctagtatt
     6661 tgcgggcttt ttttcatact atagctcttg tctttacgcc attcaatttt ttccattttt
     6721 cttcctccgc gaaattccat ttagttattt attatcatac ctctttttga gtgacttggc
     6781 taatcgtgtt gacactattt ttcagtttgt gctagaataa gtgaagtaaa taaatcgtac
     6841 agataacacg tttcggcgtg tcgagagaga ggcggtttct atttttataa tagaaatcac
     6901 tctctttttt tacgaaattt tttagaggtg atgtaaatga caaaacaaaa acagctgctt
     6961 ttagtacctg cagtaattgc aacagggatg atgtcttttg cgggcgtatt gatcgagacg
     7021 gccatgaatg taactttccc aactcttatc agacagtttg atttaacgac tgcacaagtc
     7081 caatgggtaa caacgattta tcttttgatg atttcgatca ttgttccgct gtctacttat
     7141 ttgaatcgca attttagtct caaaaaactc tttttagctt caaatctgct tttcttggca
     7201 ggtataatga tcgatttctt ctctcctacc ttcatggtcc tattgtttgg tcgattgctc
     7261 caaggtgcag cgacaggtat cgctcttccg ttgatgttcc atattatctt gacatttgcc
     7321 cccctcaatc gacgaggcgc gatgatgggt gtcggtactt tgacaacggc cattgctcca
     7381 gcaattggcc ccacttatgg aggcattatg acttcacatt ttacatggaa tcatatcttc
     7441 ctgtttcttg tgcccgtttt gtttctttct ttgattatcg gctggtttgc tattcccgag
     7501 atggcagtac aaaaaagtgg ttatttggat tggataagcg ttttaggaat ttgtcttttg
     7561 tttagtggaa gtctaacatt tttgagtacg ttgggaacag tttccagctg gattgcttta
     7621 atcatcggcg gtattggctg gttccttttt tactatcgaa gtaaaaaagt ggagcatcca
     7681 ctgatccgac tgtctattct ttcaaataaa acattccgct tgtttttgat tggcttctta
     7741 gtttatcagt ttttattact tggtgtatct tttgtcttgc cgaatttcct tcagatcgtt
     7801 caaggaagta gtccttttgt cgctggatta gcgatgcttc ccggcgcagc ggttggtgct
     7861 ttgctttctc ctttttccgg gaaaatgctg gatcaatatg gtccgaagaa gccaattttt
     7921 gctgggctga ttctttcttt gattggttgg ctagcattat tcctactcat aggacacgct
     7981 tcagtgggtg tattgattgc ttgtcatgtg ttttatatga tcggtatcgg attatcttac
     8041 agcaatatga tgaccaccgg aatgaatgca ctgaatgaag aactacaagg agacgggaac
     8101 gcaatgttca ataccttgca gcagttttct ggcgctgtgg ctacttcatt agtagcggtc
     8161 attatcaatt acgtccagca tcatacttcc cataactatg aagtgtctac tacattagga
     8221 tcaaaagcag cattaggcgt cttgttgctg ttgcttctcg taagctttgc tcgattcgcc
     8281 tattatctgt tctttgctaa aaaagcgtag attccatata cggcaggtgt tgcatctgcc
     8341 gtttttttgt gtgaaaatgg ttgtttgtaa aaggaggctt ttttatggaa aaaattgcca
     8401 ttatcggtgg cgggatcatt ggtatgaccc tagccagtta cttagataca tcaaaattca
     8461 atgttgcttt atttgatgaa gggacaggac aggcaactaa agctagcgct gggattatct
     8521 ccccttggtt gtcgaaacgc agaaacaaac aatggtatcg tctggctaaa gacggcgctg
     8581 cttttttccc taaacttata aaagatttcc aacttgatca aacgatttac gcacaatcag
     8641 ggacactttt gcttcgacct gaaaaagatt tagctgaatt ggctgttctt gccgagaaac
     8701 gaaaagaaga agcgccagaa attggaaaca tcgaatggct ttcacctgaa cagacatctc
     8761 aagcacttcc gttattgaaa ccagcagttt cactgcgtat cagtggtggt ggtcgtctgg
     8821 atggaaaagc atatttagaa catcttggtc agcgtttagc acaaaaacaa atggagcaga
     8881 tccaggcaaa agtcactttg cataagaaga gcgattcttg gctcatcaag ggaccgtttg
     8941 gagaatacac agctgatcgt gtaattttga cacctggccc ggcacttaag cctttattac
     9001 aagaattagg ctatgctgca gatatacgtc cgcaaaaagg gcagctctta gcatttcaga
     9061 catcttataa gatgagtcaa gactggcctg ttgctatgct tgacggagaa gctgatttca
     9121 ttcctttcat ggatggaaag atccttttag gtgctaccca tgaaaacgat caaggctggg
     9181 atttggctcc tactagcgaa gcttataagg gattgactaa gagtacagaa cagttcttga
     9241 ctgatccaga tcagataaaa aactggccat acgagttccg tgtaggtacc cgtgcttata
     9301 cgtcagattt ttccccattt ttcggcgtac tgccagatga tccaagctta ctggctgcta
     9361 gcggtctagg ctcttctggc ttgaccacag gtccttatat cggctatcta ttagccaact
     9421 tcttaaatac tggtcattgg cctacggaag attatcaaaa ggacatctcc acctatatta
     9481 cgaaaaatcc atcatagaaa aacagctttt tcaagtaagg atcccgagcg atattgcgca
     9541 tggattactt cttgaaaaag ctgttatttt agttcaattt ctgaatcaaa ttctccaaac
     9601 tttcttttgc ttcatctggc aagagatctt ttcctatctg agcttcaatg aatcgtaaac
     9661 gaatatcaag tcgttgtttc accagatttt tataggacgt atcctcaaaa gaaacaggaa
     9721 aatctggtat aggtaaatag tggatgtctt caaatggacc aaacgacttg aattctgccg
     9781 tttgttgcac gattctttca acgattgaca atgtcgtttc aggtttgatt tttttatcat
     9841 cgaaatatcc agtattcagg acatagcagg ctgtttgctg tttcaaaaac aactctcgaa
     9901 aagcttgata gtcttcccta agaggataga cacggaaagg attagcaaat ggttcaatga
     9961 ctagctcatc tagctgtctt tttgtagtga tattttccgc gtctgatcgt tttgttgcta
    10021 atgttgcacc aaaagacgct gctaaaactg ggtcattgat cttgataatt ggcggaagcg
    10081 aatcatcttt catgatccaa aaaacagcat cgatcttttc gctaagatga tcgactcggt
    10141 cgggtgtcgc ataacgtgat ttgactgtcc gcccatttcc gtttcgtata tctcctgtta
    10201 ctaggacttt ttctccttta cttgtacatg tcacgcccac attctgacaa gtaaggaaag
    10261 aagaaatggc cggatcatca gacggatagt cttgtgtttt atcaaagtaa gaaggttcca
    10321 aggcagtagc aaaaccattt tttcgagaaa taacgaaagc atcgtcatgc aagatcgaaa
    10381 cagagtgacc ttccgtatgt tttgctaagg taatcgttga ttttccagaa ccggacaacc
    10441 caaatacagc catcgtatag gtctggtcat tactagcata ttgtttcatc ccaccgtggc
    10501 aggcaataaa gccattgcga tttcccaatg cccaagcaag cgtcaaagtc gcttttttta
    10561 attctccaaa gtaacgtaat cctagcacag ccgctgtatt atggagagca tcaaacaaaa
    10621 tgagtccatt tggaaatgtt tcatgtgtcc attcgggatc acaaaagata taaagatctc
    10681 cctcatcaaa ggaaacagac ttttgataag tctctaagat cttcgaattc gctggctgaa
    10741 aattcagcaa ataagaatac aaattgttct caaatccttc tggaatcagc aaatgacttt
    10801 gcacagaaaa ttcttcagat aggccaacga ttacttttgt atggtaaaac tggcgcttgc
    10861 gactttgata cacagcttct cttaaaattt tctcgtattt gctttgttcc ttgtctaagt
    10921 cctcaaagat ccgccgagca cttgctgttc ttcccacaat gcttccatca ttgaatacta
    10981 gtgtcctaac atcagctggt aaatctagtt cttcagcatg tgagattgtt aaatctgtga
    11041 tgatcgttcc tggactccct tttgctagtt gataagctgt cttcctatca aaaacttcat
    11101 gaacattatt accgtaaaat gtcgtttcta caatcgatcg aatactggat aacagtggat
    11161 ttccacttgt gatttcgttt aattcgagac tctcttttgt actcatggct cctcctcatt
    11221 ccctaaacaa aaaaacgttt tctttattat acattattcg agtttttatt tgtactgctg
    11281 cgctgtggga agcaaacgag ttatttttca cacaaaaaaa ccgagaactt gaataaacaa
    11341 gtttctcggt ttcatcattt tccttttatc ggcttagtac cagccgtttg ctaaccagaa
    11401 tgttttagca gcatcccatg aaccataacg agatgttaca tattgttctg ctacacgttc
    11461 ttgattggca gctgaatagt cgccgtttaa gtatgaagca tctaattgat aacggccgat
    11521 gtaacgtccg ttagtagctg tgtatgaacc gcctgattct ttttgtgcga tccattcttt
    11581 tgcagagctt gaagttggcg ctgttgcagc agctggggca gcaggagcta cttcaactgt
    11641 ttcaacaggt gcaacgtatg tatgttcttg cgttgctgtt tcagttgtag atgctgtatt
    11701 ttctgttgtt ttgttttctg ttgttgctga agaatcattt gaagttggga ttgttaattg
    11761 ttcaccaaca taaatgcggt tgatgtttgc gatcgaattt ttttcagcaa ttgcatcgat
    11821 cattgagttg tctcctgcaa atttttgtga gatcttagat aatgaatcac ctgattttac
    11881 tgtataaact tcatcagcgt gagcatttgt agttcctaca aacattgcag tagcaccagc
    11941 agcaaaagtt gtacctaaaa caattttctt aaatgaattc atattaaact cctttttttc
    12001 tccgtttttt attctttcga cgctattaac ttta
//
LOCUS       DAYYGS010000078        11780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000078 DAYYGS010000000
VERSION     DAYYGS010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.078"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            506..1069
                     /locus_tag="ACHA44_002175"
     CDS             506..1069
                     /locus_tag="ACHA44_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905435.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(1130..1495)
                     /locus_tag="ACHA44_002176"
     CDS             complement(1130..1495)
                     /locus_tag="ACHA44_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02328 family protein"
                     /protein_id="HFM8905436.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1571..1816)
                     /locus_tag="ACHA44_002177"
     CDS             complement(1571..1816)
                     /locus_tag="ACHA44_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905437.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1886..2140)
                     /locus_tag="ACHA44_002178"
     CDS             complement(1886..2140)
                     /locus_tag="ACHA44_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905438.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(2304..2627)
                     /locus_tag="ACHA44_002179"
     CDS             complement(2304..2627)
                     /locus_tag="ACHA44_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905439.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2814..2887
                     /locus_tag="ACHA44_002180"
     tRNA            2814..2887
                     /locus_tag="ACHA44_002180"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2848..2850,aa:Ile,seq:cat)
     gene            complement(3272..3769)
                     /locus_tag="ACHA44_002181"
     CDS             complement(3272..3769)
                     /locus_tag="ACHA44_002181"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HFM8905440.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3809..4618)
                     /locus_tag="ACHA44_002182"
     CDS             complement(3809..4618)
                     /locus_tag="ACHA44_002182"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8905441.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4952..5809
                     /locus_tag="ACHA44_002183"
     CDS             4952..5809
                     /locus_tag="ACHA44_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8905442.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5802..6704
                     /locus_tag="ACHA44_002184"
     CDS             5802..6704
                     /locus_tag="ACHA44_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFM8905443.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6737..7087
                     /locus_tag="ACHA44_002185"
     CDS             6737..7087
                     /locus_tag="ACHA44_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8905444.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            7136..8059
                     /locus_tag="ACHA44_002186"
     CDS             7136..8059
                     /locus_tag="ACHA44_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HFM8905445.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            8137..9030
                     /locus_tag="ACHA44_002187"
     CDS             8137..9030
                     /locus_tag="ACHA44_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8905446.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9068..9631
                     /gene="amaP"
                     /locus_tag="ACHA44_002188"
     CDS             9068..9631
                     /gene="amaP"
                     /locus_tag="ACHA44_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8905447.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9645..9833
                     /locus_tag="ACHA44_002189"
     CDS             9645..9833
                     /locus_tag="ACHA44_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8905448.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9846..10748
                     /locus_tag="ACHA44_002190"
     CDS             9846..10748
                     /locus_tag="ACHA44_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8905449.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10780..11022
                     /locus_tag="ACHA44_002191"
     CDS             10780..11022
                     /locus_tag="ACHA44_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8905450.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(11278..11649)
                     /locus_tag="ACHA44_002192"
     CDS             complement(11278..11649)
                     /locus_tag="ACHA44_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905451.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4176 a         1984 c         2232 g         3388 t
ORIGIN      
        1 attggtcgtt gaatgggctg aatagtagtg tataaaacaa ttttgtggag cgtgaagctt
       61 ttatggtaga ggaaaatacg aataaagtga tgttgataaa aagattccta tcttgaatat
      121 gaatcgagat gtcttgaaaa tgaatgagtt tgaccaatca ttatcctatc gaaatttaca
      181 gtttcgttcg attggtctgt caattaatga aaaagtaaaa aagaatatgt tctaggaaga
      241 gggcggcaca tctaaatttt taagtaacgg acagttcaaa tatcaagcat tgaaagaggc
      301 tatcctactg tttgaaggaa tacgaatata taaaaacgac aaagaggaaa aagtagttca
      361 ttaaatccct tcaagtgatg aaagaatgag atctaataaa acctgatctt tcatctgttt
      421 ttttaataac gtaataattt ttttattttt tgtgctttcg ccgttattgt agagtgattt
      481 agcattctta tttttaaaaa taactgtgtt atatattaaa aaaggcataa tagttataag
      541 cttttttaaa gaatatttag aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt
      601 tattatttta gtctattttc agggggggaa ccttatgata aaaaaactat ttttactatc
      661 aatattaacg ctagcaatgg caggttgttc tgactctggc ttagcaactg aactgatcac
      721 aaaatcaact gaagccacga cagaggagtt aaaatgtgga aacgatcaca gtgactacag
      781 aggcttaaat cgacgatgca tgggaatatt aggcagaagc aattgtaagc atcgatgtag
      841 taatcgaaga aggtgtagaa gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa
      901 tctgtgctgt gcatcgtata cagacgtcga agatgcggta cttttaaacc acgtcaggag
      961 atgttcattt atgtctaggg aagttggcga tgaaccaaaa ctagagtacg cttttagttt
     1021 tgcacgtgag gatgaagata accctaaaac ttacgacatt ttagcttgat ctaagatggt
     1081 taaagctttt taatgaagta agaggagata gaaggctttt ctagttatat taggtaagat
     1141 aaattccttt ttgctccaaa tttgctaaac attctgctag ataaacttca tcgtgttccg
     1201 gataaatcgg cacgttgcgc tctacagtag gtaaatcaaa aaatgcttcg catgatttgc
     1261 cacgatacat aggatttttc cactctggtg ctgggctgta accacgtttt tccatttcat
     1321 ccatgacaag ctgatgatat tggaataatc tataaggaga atagtgaaaa acataatcca
     1381 cagtagcatg tttctttccc cagccatttc ctcttaatgc acagcattcc cgatgttgtc
     1441 ctaaaagctg ttgacgagga agctttgaaa tcaatttttc atgccataat cgcatagaaa
     1501 tgtctccttt atatatagat tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg
     1561 ctggaaaaaa tcacttcagt ttgcttgctg tttctgagcg accaattagg taatcaacgg
     1621 aaatatcgta aaagtcagcg atatagatta attcaatgat gcttggaatt tcttctcctt
     1681 cttcgtactt acgatatttg aataaaggaa tacttaacgc gctagaaaca gctacttgag
     1741 ataaagatcg gccagttctt aagaaattca gacgacaacg taaagtatcc agtaaaatag
     1801 cttcttcgat actcatttaa tcacctttca ttatagtaga atagttataa tgactataga
     1861 aagctacaca tgcaatcaat agtactcaat gttttttatt taaacgtatc aaataaagaa
     1921 tgacgatcaa taaagacaaa accagtcctg ataattcttc tataaaaaca ctatcgggtt
     1981 caaagtagtt gtaattaaaa actttccaca taaagtagat agccggaaga gtcaaaatag
     2041 ctaaataaaa ccgttctttg ttccaattca taagataaac aatacttgcg accagtgcga
     2101 ccgcaggcac ccaaaacaaa actctcacca acgtaatcat gtaacggact cctcaacgat
     2161 tcatataata aaagataaca tgttattgca tacatcacaa atgaaatgct aggaaaataa
     2221 aagaagaata gatcaatgaa aaatgaaaaa gcagaacagt tttctgctga taagcaggaa
     2281 ctgttccgct gaatgtgtct tatctatcga gaaaaaatca atggattgat atctaagtaa
     2341 ttcaatacat ataaaaagag gacgatcgca ttcactaaaa aggaaagaat gatgatccca
     2401 tagcgaatgg aaacatattt aacaaatact tttgtttgaa tacgggcata tctttttcct
     2461 ttatagctga gcatcatgac aagaagcaaa agggtaatca caccttgaaa aatcattcca
     2521 ataaacagaa ttcctgaggt ttgataattt aattggagaa aataagctgc tgccgcaact
     2581 aaatcgaata aaatcgcaat aaatggcaaa ataatcactc ctaacattga gaataaataa
     2641 aagaataaca ttttttctaa aataagcaaa taaaaattaa agtaacaaaa tagttatgac
     2701 acaagtaaaa gaaataagaa taacaatatt attttactta tattttgaaa cttgtgagag
     2761 tttgtcattc gtgctatagt tgaaaagtaa caaaaataaa ttttatttat gatgtacctt
     2821 tagctcagtt ggttagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca
     2881 aggtacataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaagtaa
     2941 aaacagcttc ttatgttttt acttggctgt tcttattact gcagatgcaa gcttttgaat
     3001 accgtttaag gaagaggttg tgaaatcagc gttttgacct gagtattagc ttaacaaacg
     3061 gaaaaatagt tcctcatatt ttgccgtttg ttaagctaat accgagggtc agctgattga
     3121 acaccattta agaaagaggt cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa
     3181 tctactttga tagaaaaagc acgtaggact aaaacagttc tgcgtgcttt ttttattgtg
     3241 tgttaaatat cgtaaatgaa gatagatatt tttatgattc gacgatagag acgttgaaat
     3301 ctttcaacag ctctaaagcg gattctccgt gttttctact ggagactaac attggatcaa
     3361 caaaaaagtc tgcttcagga aaagcactct gcaggcctat cagattagag agtacacaca
     3421 agtttgtttc aatcccgact acttcgatct ctttttgttc ttctttgctt tcaaataaag
     3481 tttcctggat ttcagctaaa gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc
     3541 gttcattttc ttgtggagaa agaagaggga tcaattgcaa atcttctcgt tcttcttctt
     3601 tgtccttcag ttcaactggt atatcccgtg tgaagagaat atattcatta ttttggcgtg
     3661 ctttagctaa tctttgagca atacgttctg ccaattgatc tgactctgga atataaaatt
     3721 cgctgtttgg atcaagaata tgattttgca tatcaataat aactaacatt ttctttcctc
     3781 ctacattcat atcgtcattt tattcttttt aatagaagtt ttttaaaata taagcaacac
     3841 cagcttcttc attcgtcagt gttacttggt ttgcaatttc ttttacttgt cgctgtgcat
     3901 tccccatcgc aatactcaac ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc
     3961 caaaagccat gatgtcactc gattcgattc caaggtaatc cgccagaaaa acaagtgaat
     4021 gagctttagt gatacctgta ggcgtgattt ctaagaaatg cggatcagag aatactgatt
     4081 ggataccatc tgcttgcaga tctctttgta gcttctgtag tttttcttca ctcttatctg
     4141 ttaatgctaa tttaagatag ctaagagaag gatcttccaa catatttaaa atttgatcaa
     4201 aagaatggtt ctgtaaattg aaatgttcat aataagggtc atctttgact agatttgatt
     4261 catcgaatgt cagctgatca tcttgggttt ctcggctagc aatgatggat attttcgctt
     4321 cttgagcata gaagaaggct tttctcagtg tatcgctatc tatttttgaa cgtctgagaa
     4381 ctttcgccat ttttccatcc ttcatttcag caatcagtga accattggaa gaaatcaaat
     4441 agccaccgat tcctaattcg gaaaaaatat ctttagcaga aatgctgttc cttccggtag
     4501 cgacggttac gattccgcca ttttcatcaa atttccgaag tgcttggata ttttcttctg
     4561 gtatctttcc atttcgatca agcaaggtac cgtctaaatc aatcgctgct agtttcattt
     4621 tatcttccta ctttcttttt acatatctat tttcctgtta ccttaagatt aaatgagaat
     4681 ttaaaaaaat gaaactaact tgctttgtca ttatgaattt tttcaaaaaa cattcttaag
     4741 aaaaaggtgg ctatatatag tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa
     4801 acgaatagtt agcgcttacc gatgcgtttt gtgataaaaa aattttaaaa tattcaatga
     4861 aaacaaagca tatattttgc tttaaaacca aatgaggaat attctagatg tagaacaggt
     4921 tagaaaaaga ttagaaaaag gagtgatttt gatggagaag agatacggcg tagttcctgt
     4981 ggtaactgct tcagacgaaa attatgctcc ttacttgagc gtgatgatcg caactgcatt
     5041 agaaaattgc aataaaacaa gacgcataaa attttatgtg attgacgatg gtctatcaga
     5101 atatagtaaa gaaggattgg aagaaacggt aaataaatat tcaagtaatg caagtatcca
     5161 attcctgaca gtggaaaaag atatctacga agacttccta gtcagtgacc atatcacaac
     5221 taccgcttac ttaagaattt ctttaccaaa cttattagct aaagaggatt ataagaaagt
     5281 cctttactta gattcagatg tgttagtact agacgatatc gtaaaactat atgatgagcc
     5341 gttaaatgga aaaacaatcg gcgctattat cgatccaggc caagtgaaag ctttggaaag
     5401 attaggaatc gattccgatg acttgtactt caattcagga gtgatggtga ttgacattga
     5461 tcaatggaat aaaaaagaaa ttacagaaaa aactatccat tacctcagtg aaaatggcga
     5521 tcgaatcatc tatcacgacc aagatgcttt gaatgctgta ctttatgaag attgggaaca
     5581 acttcatcca aaatggaaca tgcagacttc gctgattttt gaacgtcatc cagcaccaaa
     5641 tgaaaaatat gaacgtcagt ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac
     5701 aggacatgat aaaccttgga atacgttgaa agatcatccg tataccaatt tatacttaaa
     5761 aaaattagca cacagtactc taacgaaagt aggcgagatc aatgaataaa aaagaaatag
     5821 cagtagtcgc aagttgtaac acaaaattcg taccacattt agctgcatta tttgtctcag
     5881 tattagataa ttgcaacccg tctaaatttg ttcggttcta tgtgattgac gatgatatcg
     5941 attttgaaag taaacagctt ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg
     6001 tggaattctt gaaaatcaat aaagaattct ttacaaatgt ggtaatcagc gatcgtattc
     6061 cagaaacagc ttattatcgt attgcgattc cggaattgtt ccgcggcaca gaagttgaac
     6121 gtatcttata tatggattgc gacatgatcg ctttacaaga tatctctaaa ttatggcgtc
     6181 ttgacttcgg tgattcaatc gtagcagctg ttgaagacgc tgggttccat caacgactgg
     6241 aaaaaatgaa aatttcagct aaatcaatgc gttatttcaa ctcaggtttg atgctgatca
     6301 atgtgaaaaa atggttagat gaaaatatta cgcaaaaagt attagatttc atcgaacata
     6361 atccagaaaa attacgattc catgaccaag atgcattaaa tgctatctta catgaccgtt
     6421 ggttaccact tcatccgcgt tggaatgcac aaggctatat catggcaaaa gcaaaaaaac
     6481 atccaacagc agcaggtgaa cgtgaatacg aagaaactcg caacaatcct tacattatcc
     6541 atttttcagg acatgtgaaa ccgtggagca aggattttga agggccaacg aagaaatatt
     6601 acgaaaaata tgcagggatg acggctttcc gttgtgtagc taaattccct aaatatccaa
     6661 aatatgcaaa agttcaacca aaaacagatg tagttgttaa ataatagaag accacaactg
     6721 aagaggggga gtaggaatgg aaattacaat gtctaaaaat gcagtagaga cgttaattga
     6781 aaaggttggg gaaaatacaa aaatcgcact cgcgcttatc aatgattccg atccatttct
     6841 aagggacaaa ggtgcttttg caaaaggcag cttttttcaa atcattcctt ttgtttcgga
     6901 atttggagaa tatgcgacta aaatcgagca tccattattg gacatctata catctaaatt
     6961 agagcaaaat tatttcggta aacgattgaa catggacttc aacaaacagc ttgacagctt
     7021 ttcattagaa aatgaaatcg cagtactcga ttataatatc aaattaaaga actgcttctt
     7081 cagctaatag tttcgagaaa gtccaaaaag gaggtataca ttgagatact actttgtgga
     7141 tgaagaaaag aatgtaatag aaacagatga aaaaaaatat aattggctcg tacttgatag
     7201 tgcaaagccc gaagagattg agcaagcaat cgaagaattc gagcttcctg atgatatttt
     7261 cgtaggaacc gaatatccgg aagaagtttc tcggatggag cgcctttatg agacgaaact
     7321 agagcatcca ttttctttgg tagtgattaa tctagatcag tcagataatc gtatcgaaaa
     7381 aaaattaacg ccaatatctt ttgtactttc cgataatctc ttgattactt gtatcgatag
     7441 aaaaaccgaa tttgtggatc ggctgctgac aaagcatgga aatcatttaa actcgtttga
     7501 aaagatcatc acgtatacat tattagatat ttacacccat tacgtgaaag aactaagaga
     7561 gatgaaaaag cgaattgacg ctttggataa agcagctcgt aaaacaacgg aaaatgaaga
     7621 actgtataaa caggcagatc ttgaaagaga catcgtatat atcgatcata ccttaagaga
     7681 tcaacgggaa acaatggatc aattatggaa ttctgaaact ttcatcaaac gtttagacga
     7741 tgaacagcta ctctatgatg tccatttgag acagcgacaa acagaaaaaa tgatcgagat
     7801 ctatcgagat ttattagaga gtattggtga tctattcaac gggatgatgg acaataatct
     7861 aaatcatatc atgaaatatc tagattcagc tgcattaatc atttccgttc cggcgttgtt
     7921 cgcaggaatt tggggaatga atacaggagg attaccgggt aaaagttctg ctctaggctt
     7981 tatactcgta gtctttggct ctttgatagc agcagtagtt ttaggttacc atttataccg
     8041 gaaagattat acaaattaaa gaatctattt aaaacaggta tcaaacaaag aaatttgttt
     8101 tggcaataca atttaaaaac taggaggaat acagcaatga cagaaccaaa attaatcgac
     8161 ccgcgaaact tgtaccatac ggagaaattt cctaaacaag atcaggatac accagcttta
     8221 cagcagaata tgattcctgt tcctgattgt ggagaagaat cctatgaagg caataatcaa
     8281 ctagaaaacc gccgtgtact aatcactggt ggagattcag ggatcggtcg tgcagcagcg
     8341 attgcatttg ctagagaagg ggcagatatc gctcttcaat tcttcccagg agaagaaaaa
     8401 gatgcagagg aagtagcaaa atacgtgaaa gatgcaggac gtaagattgt cttactacct
     8461 tttgatcttc gtgacgaaca agcacctaaa gaaatcatag ataaagccgt gagtgaactt
     8521 ggcgggctag atacgatggt actaaacgca ggacagcaaa tttcttgtca gtctattgct
     8581 gagttaccac ttgagcaagt caaagacaca tttatgataa acatcatcgc tatgtttgct
     8641 ttagtgaaag aagctgttcc gcatatccca gcaggaggag caatcgtaac aacaacttct
     8701 gttcaagcat tcaatccaag cgaacattta ttagattacg cagcaacaaa agcttcaatc
     8761 gctaacttta cgatcggttt agcaaaacag cttgcaccta aaggaatccg tgtaaatggt
     8821 gtagcacctg gtccaatttg gacaccatta caactagacg aaggacaacc ttctgattcg
     8881 atccctgaat ttggtcagca aagtttacta gaacgtgcag gtcagccagg agaattagca
     8941 ccagtttatg ttttcttagc atcgaataaa gcaagttatg ttacagccca aatttacgga
     9001 gtaactggtg gagaagcgat caacttataa gaagttgatc gtttcacccc tatgaggagg
     9061 tgcgaaaatg aataaaggtg taaaaacgat cggagtgatc ggaacattac ttttgctatc
     9121 tgtgcttttg tttgcgatac tattaagtgc aacctatcca ttgccttttc agctagagaa
     9181 gtttcgattc ttttccatca ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt
     9241 tgcatttgct attttgacaa ttatcacgtt acttgttttc ctgttttatc caaaaacaat
     9301 cggaaacttt ctactgaaac atggtgatgg ggagttgacg ttggataaaa aagcaattga
     9361 aggaatggtt cgttctcact tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt
     9421 caaagcaacg aaaaacaaga tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc
     9481 actcattggt aaaacaggaa cattgatgaa agaaatcgaa aatgaagtac aacacatatt
     9541 gggaacaaaa gagccagtga aagtatcggt gaaatacaca ggctttgaag aaccagctaa
     9601 acgaagcaat gaccgcaaac gagtagaata gggggggacc aaaaatgaaa gaactatttt
     9661 cgacatacaa gctccctatc atctttgggg tacttggatt gatattagcc gttttatgga
     9721 tcaccgtggg ctttttcaag accttattga tcttgatcat ggcagcgatc ggcgtcgcta
     9781 tcggttttta tctgaaacaa acgaaaatct tagatcacta ttttaaatct taggaggaaa
     9841 catccatgaa taacaataac aaaaataata acaataaaat gaacaaccaa aaaccagtgt
     9901 ctgcacatcc agacccaacg acaccaaacc aatcaaatgg tgtgaacgat gaaaagaaca
     9961 aaacagcacc agtatctgca catccagatc cagtagtccc taacgttcaa caaggagtag
    10021 aagagaagaa acatgatgca gaaaagcatg cacctcatca tccaggaact ccagaatcta
    10081 aagttccaga acaacatgat gcaatcgatc cttctaaacc tgtggaagag caaaaacatc
    10141 cttctgacca tgcgaaagca caagaaaaac ttagttctga ccacacaaac ggacaaggaa
    10201 aacttggttc tgatcataca aataaccaag gaaagcatga ttctgaacag gaagattcaa
    10261 tgcgcggtga gctgaattat gaagataaag ttgtgcaaaa aatcatcgga atcgcaattg
    10321 aacaagtaga cggtttatta tctgcaaacg gtggtttctt ctctaatgtg gcaggaaaac
    10381 ttgtgaatac agataatgta acggctggga tcgaaacaga agtcggtaaa aaacaagtag
    10441 cagttgatat ggatgtcatt gtggaatatg gcaaagatat cgaaaaaatc tttgaagaaa
    10501 tgcaagaagt aatcggcaaa gaagtaaaaa atatgacaca tttagaagtg atcgaagtca
    10561 acgctaacgt agtggacatc aaaacaaaag aagaatttga aaaagatcaa gaaacggtgc
    10621 aagacaaagt aactgacgca gcgaaaaaaa ccggtgaatt tgcttcgaat caaacagata
    10681 aagtaaaatc agcagctgga agcggcgcac aaaaagtaaa agaaaataca gaaccacggg
    10741 ttcagtaata aggaagtaaa acattaggag gaacgaaaca tgggatttat ttggtcactt
    10801 atcgtcggag gaattatcgg agcaattgca ggagcaatca cgaataaagg ttcttcgatg
    10861 gggattattg caaatgtcgt agcaggttta gttggatcag cacttggtca agcattatta
    10921 ggttcttggg gaccaagtat tgcaggaatg gcaatcgtgc catcattgat tggtgccatc
    10981 attttagtac taatcgtatc attctttgct cgaaaactgt aaaaagactt ttaaaaagag
    11041 gtcgtgaaaa agctgttctt catcaagtaa taagaacagc caaacaaaaa tagcttctca
    11101 tatttttcat ttggttgggc atattatcga agatgcaagc ttttgaacac tgtttatacg
    11161 gaataagagg ctgtgacaag acttttgtca cagcctctta tttatgcggg caaattccca
    11221 attagatttt taaaagtttt tccttaaatg tgtctttatt gatacagaga agcttcttta
    11281 ataaaacaat gcctccctaa attttcttga gataggaaag gtgaacatac aaaataatac
    11341 gaccactttg aaaacccaga tgatccacaa atggatcgta tgatactgat gaagtaaaaa
    11401 gcaatggatc agaaactcaa agagattgaa aaagacaagg aagaaataat atctcttttt
    11461 tactcttgtc tgttttagcc aataatagct cgtaaagtaa tctagacaaa caaatagaag
    11521 taaacaaagt ttcgtaagag cagcaatcgt atcatgttgc tctgctagac gattgacgag
    11581 caggaaaaat gttggtaatg gaatatagat cacgagtaat gaaagaaaaa agtaaacaat
    11641 aaatggcata ctattcctcc taattgtgtg agttcgccct cattttaaaa taacgatatt
    11701 tgtttgtgaa gctttttcgt gtcgtaacaa atgttatcta atgtattttt aaataaataa
    11761 tcattattat caaaataatg
//
LOCUS       DAYYGS010000079        11755 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000079 DAYYGS010000000
VERSION     DAYYGS010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.079"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..177
                     /locus_tag="ACHA44_002193"
     CDS             <1..177
                     /locus_tag="ACHA44_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905452.1"
                     /translation="YLLGMALAYSVGSLIKVNVSLDLFTILLAVGVSSVIGLVFSVMP
                     ASEAAKKDLIDILR"
     gene            578..1183
                     /gene="fic"
                     /locus_tag="ACHA44_002194"
     CDS             578..1183
                     /gene="fic"
                     /locus_tag="ACHA44_002194"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFM8905453.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1199..1771
                     /locus_tag="ACHA44_002195"
     CDS             1199..1771
                     /locus_tag="ACHA44_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905454.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2023..2280)
                     /locus_tag="ACHA44_002196"
     CDS             complement(2023..2280)
                     /locus_tag="ACHA44_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905455.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2406..3221)
                     /locus_tag="ACHA44_002197"
     CDS             complement(2406..3221)
                     /locus_tag="ACHA44_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905456.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            3738..5063
                     /locus_tag="ACHA44_002198"
     CDS             3738..5063
                     /locus_tag="ACHA44_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8905457.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5056..5406
                     /locus_tag="ACHA44_002199"
     CDS             5056..5406
                     /locus_tag="ACHA44_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905458.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5403..5573
                     /locus_tag="ACHA44_002200"
     CDS             5403..5573
                     /locus_tag="ACHA44_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905459.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHA44_002201"
     CDS             complement(6477..6752)
                     /gene="prgO"
                     /locus_tag="ACHA44_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA segregation protein PrgO"
                     /protein_id="HFM8905460.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(6724..7677)
                     /locus_tag="ACHA44_002202"
     CDS             complement(6724..7677)
                     /locus_tag="ACHA44_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8905461.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8289..9782
                     /locus_tag="ACHA44_002203"
     CDS             8289..9782
                     /locus_tag="ACHA44_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HFM8905462.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            9917..10213
                     /locus_tag="ACHA44_002204"
     CDS             9917..10213
                     /locus_tag="ACHA44_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8905463.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10314..11165
                     /locus_tag="ACHA44_002205"
     CDS             10314..11165
                     /locus_tag="ACHA44_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905464.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11186..11398
                     /locus_tag="ACHA44_002206"
     CDS             11186..11398
                     /locus_tag="ACHA44_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905465.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT         4041 a         1811 c         2179 g         3724 t
ORIGIN      
        1 tatttattag gaatggctct tgcttatagt gttggttctt tgataaaggt aaacgtgtca
       61 cttgatctat ttactatttt actagcggtt ggggtatctt cggttattgg actggtcttt
      121 tctgtaatgc ccgcttcaga ggctgcaaaa aaagatttaa ttgatattct tagataaaat
      181 cagaatgaca agaatattta cgttctagat tctaaaataa aacacaaaga gaccaaccag
      241 gcaccctatt gtttctttgt agtatagaga tattctttgg cttgtaagag atcaatagag
      301 ttaggtgacc ggagttcgaa ataaaggaaa tttttgattg tataggagaa ttctttatcc
      361 ctaatattta gaattttgag tatgccatgt tgatgtttta gattctctat aaatatagag
      421 gtgtatcaaa ggtcaactag actgtgcaaa atagagacac ttgaaaaatc tatcgggggt
      481 cagaaactta atagtttttg acacataaac aaaatcgcga tataatgggg ttgagagtgt
      541 ttcataaact tatgtcataa agttaaggag atttcgcatg attttagaga ataaattggg
      601 attaaccaat caagttgagt tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa
      661 agaattgtac gattgtggaa aaataaatga aatcgaagtt ggtacattta aaggtttatc
      721 tgaaattcat gaatttctat tttcggatat ttacgatttt gccggaaaaa tccgatcggt
      781 caatattgca aaaggaaatt ttcgatttgc accagttatg tatttagaac attcgttaca
      841 gcatattgat cagatgccac aaacttcttt tgaagagatt atcaagaaat atgtggagat
      901 gaatatcgct cacccatttc gagaaggaaa tggccgaagt actagaattt ggctggatct
      961 cattttaaag gaagaattgc aaaaagttgt agattggaac ttaatcgata aagcagacta
     1021 tctttctgca atggagcgaa gtcccataaa cgatttggaa atacgctatc taatttccaa
     1081 tgctctgact gataaaattg acgatcgcga gttatatatg aaaggaatcg atgtcagcta
     1141 tttctatgaa gggtattctg agtatacgat agatgatttg taaaagtagg tggaccaaat
     1201 gacgaaatat ggatatgcac gtgtctcaac cgcttctcaa agtacaaaag aacaaattca
     1261 acagttgctt gagaacggcg tagagaagaa gaatattttt tcagaaaaat ttacgggtac
     1321 aaaacgggat cggccaaaat tcaatcagct gttggctggt ctgaagcctg gagatgaaat
     1381 agttgttacg aagcttgatc gatttgctcg taacacgaga gaagcattag agatcattga
     1441 acctttactc gatgataacg taacgatcaa ggttttgaat ctaggaacga ttgaaaatac
     1501 gtccatgggg cgaatggtga cacggacgtt gttgagtgta gcagaaatgg aacgtgatat
     1561 gattgtggaa cgtacccaag aaggaaaaat gtttgcaaga aagaataatc ctaatttcag
     1621 agaaggacgt ccgaaagcaa caatcacacc gaagaagagg cacgcatatg aactgcttac
     1681 ttcaggtaaa tcgtataagg aagttgaagc tataaccggt ttcagtcgca gtacgctttt
     1741 tagaataaag aagaaaattg aagaaagtta aaaaagaagt ctcagcgaac cagacaggta
     1801 aatacttaat gcgttctaga tatgagtcta ttagcatgtt gtgaaatata atattttttt
     1861 atttacttga tttctgctca aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa
     1921 ggtatgctag ccacgcttac tatattagga taccatcata tatttataat gaacgatttg
     1981 ttcgtctacc aaacaacaca tatcgttcag cgtaagtcac gtttaggaac gtggcttttt
     2041 tagtgttatt atcgataaat aatctattga ctctattgat tttatctcca tatccctacg
     2101 gtgttgattg tctttagatc tttcatttaa gaaccaaata actatcataa aaatcagcac
     2161 tgctgctaat ataataaaca gcattcctat tgtgatatta cctattgatt ttaccataac
     2221 tacgattaaa gtaataatgg cattcaagtc tcctttgttc tctatatata aattagccat
     2281 aaatcatctt cctttcaaat taagatactt gtttgatacc aataaatagt aagcgtggct
     2341 agcatacctt tttctgtaat agcaagtgtt ctaatagtcc aaacttaatg atggaccgat
     2401 gctagttata gtacagtaat gttttctttc ttatctagat ttttagtaag ctctatcagc
     2461 aaatctaaca aatctgagtc cttttcagaa actaaattaa atctagtgtt tacaacaaag
     2521 ttttcatgtt ctatattgtt tagattattt gcggaaagga gggcttctag atcttgacat
     2581 tcaaatctat atgcatcgtc ataaatctta ctagaaggag tgcttaattc gaaattactt
     2641 attttctcat cttggaccaa gataaattta tagatagtat ctttttgtgt ttttctttta
     2701 aaaacacaac agagtgtgtt ataaggaacg ttataatttt catcagcctc attttttctc
     2761 cattcaaaca aatgataaac tgcaaccaag ttgccatcaa tttcatggac agtgctctcg
     2821 gtaacatcct ttaaagaact atcaagggct aatttatagt aactagagag aggaagtaaa
     2881 attgtcgatt tactgagatg gttatcgtct atacgtgcaa tcatacctaa atcaactaag
     2941 gttttattcc aggtgtttat agttttagtt gtcactccta gagactctgc tatggtattg
     3001 accgaatgcc aggaatcccc gccttcattc tttgccttaa aacagtaata cagatataaa
     3061 ttaatagctt tgttattcgg taaatatttt tcaagttctg ttggtagaat aaagtaagaa
     3121 tccttaatgt tttttctgaa ctgtctccag tctctgtagt tttgtttata aagttgtgaa
     3181 acggtttttc cacctttaga aagtcctgct atcttaacca atcttattgc tcctttcttg
     3241 aatctgtcat gatcatacag tattttttac ttgtagtcca gcaccttaat atattatcct
     3301 atgtaattaa tttcgtacta gatagtaatt attttttatt taattctcta gtcacttaca
     3361 aataagttat ataaactacg attaataaat tattacaaaa actattaaga aaacttctac
     3421 atacaactat tacaaaacta ttaagaaact gaatactcct attttttgtt tttaaggaaa
     3481 gttttttctt ttttgaattt cttatttttt tctaatataa ctaagcaaaa ctggataata
     3541 cgacacttat tttcaaattt tcacaagact aatagtaaat atccatttaa taaagtgata
     3601 caatgtgttg attttacaaa tcaacagaag aagtagggaa ataacttacc tatgcttctt
     3661 tttttgtaca tttaatcgat tcttctttac aaaaacgaac gtatgtttgt ataatgtgga
     3721 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttga
     3781 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aaggaattta gatccattaa
     3841 cgactgaatt agtcgtcatg agtcgagcgg ataatacagg atcagggttg attttagctt
     3901 cttcaccagc tgcaaagaag aagtacggta tttctaatgt cagtcgacca cgagatctac
     3961 cgcaaccttt tcctaaaacg ttacatgtcg ttccaccacg catgaatctc tatattaagc
     4021 gaaatatgca ggtaaacaat attttcagaa ggtatgtagc agacgaagat ttattgattt
     4081 actcgatcga tgaatcgatc ttaaaagtga ccagatcaat gaatcttttt acgactgaag
     4141 gaacaagaag tcaacggaga aagaagctgg ctcaaatgat ccaggagaat attaaagaag
     4201 agctaggatt gcttgctaca gtaggtgtcg gagataatcc cttattagct aaacttgctt
     4261 tagacaatga agcaaaacat aatgatggat tccttgctga atggacgtat caaaatgttc
     4321 ctgataaagt atggaatatt cctgaaatga cagacctttg gggtattgga tctcgaatga
     4381 aaaaaagact caaccaaatt ggtatcatga gtattaaaga cttagctaat tggaatccat
     4441 acactattaa gagccattta ggtgtgattg gcttacagct gtattttcat gcgaacggga
     4501 tcgatcgaac agatatagcg atcccaccag aaccgacaaa ggaaaaatca tatggtaatt
     4561 cacaagtgtt accacgagat tataccagga gaaatgaaat cgaactggtg gtaaaagaaa
     4621 tggcggaaca agtagctatt cgtatcaggc aacataactg taaaacaggt tgtgtccatt
     4681 tatacattgg cacgtcgatc ttagaaacga gaccaggttt ttcacaccag atgaagatcc
     4741 ctataacaga taatacaaag gaattgcaga attactgtct tttcttgttt gataagtatt
     4801 atgaaggaca agaagtaagg cacgttggaa taacctatag caagcttgtg tacactgatt
     4861 cgcttcagct ggatttattt agtgatccac aaaagcaaat tgacgaagaa aatttagata
     4921 aaattattga caagattcgg caaaaatatg gttttacgtc gattgtacat gcttcttcta
     4981 tgttagaagg cgctcgtagt attactagaa gtacattggt tggtggccat gcaggcggcg
     5041 caggagggct taaaaatgat tgattataat gatcgtggac gtttaaagtg ggcaggtttt
     5101 tatttatctg atcatactga aaaaattgat gccgacaatg aacaacgagc caatcaaaat
     5161 ttggcaaaag aacaaatgac gactgaagag gttgcggaag tattgaacgc tgcagtcata
     5221 aaaaatagat cagtatgtat tcaaaaagaa gaacgaaacg ctgaaggata ctatccagaa
     5281 gatattgtta gcaagatctt aggtagtgat gagctgggga tctacgttgg aaaaaacaaa
     5341 gttcactacg acgaaatacg tcatatcgaa ttttaccagg ataataaatg gggtcgatta
     5401 gaatgaacat tatccagcaa tatgagctaa agtatataac ctttgatcag ctatcagaag
     5461 agatttgggg ttatggccag cgtttgataa atgaggttgg tgttgaacgc ttctctttct
     5521 atgtagaagc tgcagcagga taccataaat acgaatacta tattggctat taaagtgcaa
     5581 taaaattata tttcttttat gttttacatt ttgtatcaat tctgataaaa ttacctatag
     5641 gtaattaaaa aatataaaaa aactagttac aaccttagga cgttgtaact agcttgaagc
     5701 atactagagc taactaaaat agcactagaa agcaatgtta aacccgtcca acaaacttga
     5761 tttaacattg agaccagctt attcagctaa atatagttga ataagcttct tttgttttaa
     5821 aaaaacaaaa ggtgtactac gaataacgca agtaaaagaa atactattac aaccagtagg
     5881 ctacaagtaa gtattactaa acattgcgta gttagaacct ttgctctatt gtctaaagcc
     5941 acaaaattag agatcagctt tatgatgcta agagcgagct gcatccatgc ggacataggt
     6001 tcttttgaca ctattaattc tcctttttag gataagggaa attgctagta gttttttcat
     6061 tagcaccatc ccccttcatt tgattgagtc tagtgctgct gtgatagttt gctgtttaat
     6121 atgcttacat tcttattata acataaatga aacaaacaca tggttagagg tcgtatcaat
     6181 caaaatagtg tctcacaaac gatttattac ttactcctgc tgactaacgg actgttcttc
     6241 agtaactgta actatatgtt tgttaaaacc accattacct gtagataaaa accaatatat
     6301 ttactatact cactatatta aatatattta gtatagtaaa aactagaaac aataaaattt
     6361 gtgaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
     6421 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
     6481 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
     6541 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     6601 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     6661 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     6721 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     6781 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     6841 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     6901 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     6961 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     7021 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     7081 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     7141 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     7201 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     7261 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     7321 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     7381 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     7441 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     7501 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     7561 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     7621 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     7681 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     7741 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     7801 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     7861 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     7921 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     7981 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     8041 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     8101 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     8161 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     8221 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     8281 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     8341 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     8401 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     8461 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     8521 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     8581 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     8641 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     8701 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     8761 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     8821 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     8881 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     8941 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     9001 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     9061 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     9121 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     9181 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     9241 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     9301 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     9361 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     9421 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     9481 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     9541 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     9601 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     9661 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     9721 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     9781 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     9841 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     9901 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     9961 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
    10021 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
    10081 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
    10141 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa aaaggaagaa
    10201 gtatatccag tagtagtttt tatttactta aaaaattggt tcattgagca acctatcgct
    10261 gctaaagtag caaaggaaaa cgttaaagga aagaatttag ggaagaaatc gaaatgaaat
    10321 cggacgactg gattgaacac acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat
    10381 tttgggtatt gcaattatgg ccattaggaa aactttgtag ttacggggca cgacttattc
    10441 ttgatttaac cgtttactcg attggagtat ttggaacggc ttattacttg ataaatgatc
    10501 ccaataggat tgcagcatca gacattttta cagtgctaat gacgttgatt tttgtcatgc
    10561 tattgtctat ttttactcaa ttagtcatgc aatttaccag gcaaaaatta acagcaggaa
    10621 aacttttcaa aagcctattc gatttaattg ttgtaaatgc aattctagcg actttaataa
    10681 aacaaattga acaatctgga agaacaacaa aaggagtgaa ggatttttta ttgacgttta
    10741 ttcactttgt aaatggacac atgtatttgt ttgaagtggg tattattctc ttttttgtcg
    10801 ggtatgtatc cacgaaactt attaaacgtt caattgaaaa agaaattgaa attgacctga
    10861 ttccgacact tcttggttat gagtacaatg ttgattttgg aagatatgac gaattgatca
    10921 atacggatat cgttcacacg agtatccatg aatctactgt taagggcatc agatctattt
    10981 taaaaaatgg ccaggaaatg aaagcaatag atcgaagaaa agagatctac aatttttatg
    11041 aagtcgaagc aagcctacga tatggcatcg gaaaattgtg gttagatgga agtaatattc
    11101 gtcaaaacaa accatacatt ttttggaaca gtagtgattt agaacttcca atgaaagata
    11161 aataattatg gagaatcgtt tgaaaatgat tcctctatac aggattgtgc ttatagttgg
    11221 catggagtta aaaaccttaa agaaggagca gttattcaaa gtgaaagaac gtcataatca
    11281 gttactatgt agtaaaacgg gtacgatgaa cattgaatac cgaacagaat atttagaatt
    11341 aacgaatcaa actgattttt tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg
    11401 gctacttata gccaattgat tgatgaatgg gaacaattca aggaatctaa aattccagaa
    11461 cgtctctaaa agcctttatt tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa
    11521 aagtgttcca ataggctgtg cctattacgt ttctcaaaaa taaatgtggg aacaaaatat
    11581 ttaaaaatag gatagtgaaa ggatagtgaa ggatagtaaa aggagggtag taggatagca
    11641 aaaaggatag gctccttttc gctatccttt ttaaattgga ggaattaata tggaagtaag
    11701 aataagaaat gtggatgaga aaatcgtaag agaaattgat cgaagatgtg acgaa
//
LOCUS       DAYYGS010000080        11742 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000080 DAYYGS010000000
VERSION     DAYYGS010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.080"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(233..2308)
                     /gene="glyS"
                     /locus_tag="ACHA44_002207"
     CDS             complement(233..2308)
                     /gene="glyS"
                     /locus_tag="ACHA44_002207"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFM8905466.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2310..3227)
                     /gene="glyQ"
                     /locus_tag="ACHA44_002208"
     CDS             complement(2310..3227)
                     /gene="glyQ"
                     /locus_tag="ACHA44_002208"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFM8905467.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(3613..4425)
                     /gene="recO"
                     /locus_tag="ACHA44_002209"
     CDS             complement(3613..4425)
                     /gene="recO"
                     /locus_tag="ACHA44_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFM8905468.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(4568..5467)
                     /gene="era"
                     /locus_tag="ACHA44_002210"
     CDS             complement(4568..5467)
                     /gene="era"
                     /locus_tag="ACHA44_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFM8905469.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5482..5877)
                     /locus_tag="ACHA44_002211"
     CDS             complement(5482..5877)
                     /locus_tag="ACHA44_002211"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HFM8905470.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(5861..6337)
                     /gene="ybeY"
                     /locus_tag="ACHA44_002212"
     CDS             complement(5861..6337)
                     /gene="ybeY"
                     /locus_tag="ACHA44_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFM8905471.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6354..8546)
                     /locus_tag="ACHA44_002213"
     CDS             complement(6354..8546)
                     /locus_tag="ACHA44_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HFM8905472.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(8570..9541)
                     /locus_tag="ACHA44_002214"
     CDS             complement(8570..9541)
                     /locus_tag="ACHA44_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFM8905473.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10247..11626
                     /gene="brnQ"
                     /locus_tag="ACHA44_002215"
     CDS             10247..11626
                     /gene="brnQ"
                     /locus_tag="ACHA44_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8905474.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
BASE COUNT         3199 a         2507 c         2152 g         3884 t
ORIGIN      
        1 ttttcttgac tagggaaatt cctaatttaa aagtaaataa aacagtaaca agatcatgat
       61 taatcgtttt catttttatt tcctctcttt tcatgagcat tttgaaaatt cgaacaaaga
      121 ataaaataac aaaaattatt aaaatagaat taataacagc tatttatatt tataacaaaa
      181 aaacagagac acagcacgtt tcgtgcatgc tctctgtttt tcaaacaaaa gactattttg
      241 tgatgatttg atccaagctt gctaatgaca aagccatggc agctatttga ctaagttgtg
      301 ttagtcggtt cttacgtact gctggttcat cagccatgac catcgtttga tcaaaataat
      361 tttcgatcag tggacgaaga gcgacaagct ggtcgtagtt ttcgtggatc gtttggtcag
      421 caaaacttgc tttcattgct tctactgctt tgtgtaattc tttctctgcg tcgttttcga
      481 acagctcagg atctacttct gattgttttt caagctgcgc ttttttcgct aagttcacga
      541 cacgtgtcaa agcttccatt gaaggtttga agtcttcatc tgcaagatgt tgtttgaaca
      601 attgtgctgc tgcaaatact tttgtcagat ctttttgttc cgcattcaaa acagcatcga
      661 tgacatcatg acgaatattc ttcgttgtca ataattggcg cagacggcct ttgataaagt
      721 cgatcacttg ttgttgccct tctgataaac caatgccgaa acgatccgta tctttactga
      781 taacatcgct aatcgtttct tgcagaacag acaaaggaaa ggcccatttt ttcgattcga
      841 tgatccgaac gacaccgtat gcttggcgac gtaaggcata aggatcgttt gaaccgcttg
      901 ggatcatccc aacagcaaag aaagaaaaga tgctatctaa tttatctgca atcgctaaaa
      961 cagaacctac tgtagactga ggtagctctc cttcactgga tataggcatg taatgctcac
     1021 gaatcgctgt cgctacagca ggtttctcgc ctttcagcag agcatatttt tcgcccatga
     1081 tcccttgcag ttcaggaaat tcccctacca tgttggttac tagatcaaat ttatagattt
     1141 cagcagctcg ttgcagatcc atcaattctt cctcagatag tcctacttgt tcgccaatga
     1201 tacgggcaat gacttgtacc cgttgcattt tctcatagat actaccgatt ttttcatgga
     1261 aagtaacagt ttttagtttt tcaacgtatt ggtcgattga agctttcttg tcttcattat
     1321 aaaagaattc cgcatcttct aaacgagccg tcaatacttt ttgattgcct cgaaccacat
     1381 tttccaattt tacccgatca ccattacgag cagaaataaa atgaggaagc agcatacctt
     1441 gatcattccg tacctcgaaa taacgttgat gttctttcat agaagtgact agtacttctt
     1501 caggaacaga aagatatttt tcttcgaagt cgccagaaaa gacagttgga tattcgacta
     1561 aattgttgac ttcttctagt aaatcttcgt ctagatcaat aacccaatga tttttttcgg
     1621 ctaattcatt ggcttgtgta acgatcattt cttgtctttt ttgcggagac gcaatgacga
     1681 attgttcatt caatttttct tcatattctt ttgcatgcgc aaatgtcaca tcttcgccta
     1741 agaaacgatg acctcgagag gctctgcctg ttttgacatc caagatttcg aatgggatta
     1801 cttcgtcatc taacaatgcc acgatccagt gaatagggcg gatatattca aaatcgtatt
     1861 ctgcccaatg catcgttaca gggaaagtca ggcttttaat gacatctgct aaacctgtca
     1921 atacttcttt ggattcctta ccatgagtgt atttcgttac atagacatat tccacacctt
     1981 ttaattctct aaaagtgata gcgtctgtcg tcagaccttg tccgcggaca aatccttccg
     2041 ctgctttcgt ccagtttcca tctgcgtcca aagcaatttt tttggctggt cctttgactt
     2101 cttcttcact gtccgcttgt cgttctggaa tgtttgttac tttgattgct aaacggcgcg
     2161 gtgtggaaaa tgtttcgatc gtatcataag tcaattggtt ttcatctaaa aattttgctg
     2221 ttttttcttc taattgtttc atgcttggtg tgacaacatg agcaggcatt tcttccagcc
     2281 cgatttcaag taataagtct tttgccatct tattcagcct ccttcacgtt ttctggattt
     2341 gctgtgtttt tattcaacaa agggaatcct aatttttcgc gttctgccac aaagattttg
     2401 gctacagcac gagccatgtt gcggatgcga gaaagataac cggcacgctc tgttacggat
     2461 actgcaccgc gggcgtctaa taagttgaat gtatggctac atttcaagat ataatcatac
     2521 gccggatgaa caagattttc atcgatacag cgtttagctt ctttttcaaa acgatcaaag
     2581 ttgcttagta gcaagtcttg gtcgcttact tcaaaagaat atttagaatg ttcatattct
     2641 ggttgtttga agatctcgcc atatttcacg ccatcagccc attctaaatc atagacactt
     2701 tcaacttctt ggatataaga agcaagacgt tctaatccat aagtgatctc agaagttact
     2761 ggtttacaag gcagtccacc aacttgttgg aagtacgtga attgtgtgat ttccattccg
     2821 tcaagccata cttcccaacc cacaccggca cagcccatgg atgggttctc ccagttgtct
     2881 tcaacaaaac ggatgtcgtg ttctagtgga tcgattccta aaaggcgcaa gctttctaaa
     2941 taaagctctt ggatattctc aggtgaaggt ttcatcacga cttggaattg atgatgctga
     3001 tacaaacggt ttgggttttc gccataacgt ccatcagctg gtcggcgaga tggctctacg
     3061 taagcagcat tccatggttc tggtccgatt gcccgtaaaa aggtatatgg gctcatcgta
     3121 cctgctcctt tttctgtgtc atacgcttgc atcagcatgc agccgtttga tgaccaaaat
     3181 ttttgtaatg ttaaaatcat atcttggacg gtcaattttt tactcatctg ttttgcccct
     3241 ttcaatttta aagcgttgct gtcaaataag aatacaggaa agaaaacgac aaaaaatccc
     3301 tatgtctgat tacagacata gggacgaata attcgcggtt ccaccctact tccgaataaa
     3361 gattcggcag cttcatttat cgtactcgca agtgcctctt caaaaatcgt ttgggtccgg
     3421 ctcccactct cccgaactcg ctaaaccacg tgttttttac tgatcttggt caatgtacgt
     3481 attcattttg cagttttctt gtttctataa tagatcctct cagtgaacga tctatttgat
     3541 ctacggttaa taatagacgg aactccctgt gtttgtcaaa tggttttgat tttttgttgt
     3601 atgtttcatt tactattctt cttttttagg tagatctttt tcagacatgt ctttctctga
     3661 cgtctttaat acgtgtgccc aagacttcat ttgatcaatg aactttttgc ttttcaaatg
     3721 gataccgaca tattcatcat acagctggtc gatgagttca cggattgctt gtttggtttc
     3781 ttctttcaga tggattccac tgattttatc ataagaaata gctgaaaaca tccgaacgaa
     3841 ataaacagct cgaggatctg catgataacg gtgatcatcc atcgaccagt gtctttcgca
     3901 tatcacacca ccatatttgg aagaatagtc aaattttcct tttgtttccc cacatacgac
     3961 gcagtgtgtc cagattggtg caattccgaa gcgttgcagt atttggactt caaaaatatt
     4021 cgtgatgatt tctgcatcat ctccttggtc catcagttgc agtgcttgtt gcgtaaactg
     4081 gaacaaatgc gggtcgtact gatgatcttc gattgccgca tctactagat tcaataaata
     4141 tgtgccatag ccgctcaaaa agatgtcttc ttggatgtgt cgaaacgcgt ggacatcttt
     4201 gacactgctt aaaaatgaca aaccttcact tcgaaaattc ccgatataaa ctgcttcagt
     4261 aaaagggagc aaagccggag ctaagggctg attttgccga tggatccctt tgatataaaa
     4321 catcattttg ccagaagatt ccgtaaagat tttgactaat ttatcttttt ctttgtaatt
     4381 acggctagat aaaatcaagc cttttgattc tgcaagctgg ctcatattct ccctcctaaa
     4441 aaataaatct ggaaaagcag attctgccga tccagatcat ttatgttgcg atcttccgca
     4501 gcttttctca acacgcggat ttttatccag tttatttcta tacctaactt acaagaagct
     4561 cagaatatca atattcgtct tttcggtagc cgaagtcttg cagatagact tttttgtctc
     4621 gccagtcttt ttgaactttg acccaaagtt ctagaaagac tttgtctcct aaaagatttt
     4681 cgatatcttt tctcgctttc gttcctactt gcttcagcat ttttccgcct ttgccgataa
     4741 tgatcccttt ttggctatct ctttcgacaa taatcgtagc ctggatatgg attttatcat
     4801 tctcgtttcg cttcatcgaa tcaacaacga ctgcgacaga atgaggcact tcgtctcgtg
     4861 ttaaaaacaa gactttctca cggatcaatt cagaaacgat gaagtattca ggatgatcag
     4921 tcacttgatc atctggaaaa tattgtgggc cctctggcat ttcactgact aatacatcca
     4981 tcaaacgttc gacgttgttt ccttctgtag cagaaattgg aatcacttgt gcaaacggca
     5041 tctgcgttga ataatcttcg atgattccca acaactcgtc tggatggatt ttatcgattt
     5101 tattgataat cagataaaca ggagaatgca cgttttttag acgttcaata ataaaatcgt
     5161 ctccccgacc gcgtttttga tcagcgctga tcataaataa gaccgcgtcc acttctttca
     5221 acgcactgta agctgcttct accataaaat ctcctaaacg atgctttggt ttatggattc
     5281 ctggtgtgtc aataaaaatc aactgagctt ctggtgtagt atatactcct tggattttat
     5341 ttctagttgt ctgcgcctta tcactcatga tggcaatttt ttgacccacg attcgattca
     5401 ataatgtgga ttttcctaca ttcgggcggc cgacgattgc gacaaaccct gatttatgtt
     5461 ctggcatata cgttctcctt tttaattaaa caacaaatga tatattttag ggacaaataa
     5521 aatcaaccca acgatgacgg aaaaacaagc ggtcactaaa actgctccag ctgccatatc
     5581 tttgattttt ttcccaatcg gatgaaaatg aaaatccgta aacatgtcca cgacattttc
     5641 aaaaacagta ttgatgatct ctactatcca tacgagaaaa acagctaaaa gcagccataa
     5701 ccattctatc cgatcaagct ggaaaatcac gcccataaac aaagcgagga ttccgaacgc
     5761 aacatgtttc ctcatgttgc gttcttcctc aaaaacggtt ttgactcctt ggacggcaaa
     5821 ttccacggac atcaggaaat gtttgttttt ctctacgttt ttatctttta agtccatagg
     5881 catctaagat ctctttctgt aaaccaaaca tttctttctc atcctctggt gtcatatggt
     5941 catacccgtt gatatgcaag aatccatgaa ccgctaaaaa gccaagttcc cgatcatacg
     6001 aatggccgta agcttctgct tgctctgcag cacgttcagt agagatcatg atgtctccca
     6061 gtacacgagg caatggattc tctccatctt caaaaatcac tgggatttcg tcttcgccct
     6121 cttcttccaa ggcaaaactg atgacgtctg tcggtgcatc ttttccacgg taatcacgat
     6181 tgattacttg gatcgctgca ttgtccatga aagtaacaga catttctgtt tcttctggca
     6241 actctaaata tgatgctgca aattgcagca agtcgtcgat ttctttcatt ttctcttctg
     6301 agacttggtt tgtttcatcc atgaaagtaa tatccattat acatcgcttc tttctattct
     6361 tcttgttctt cttttatctt ctcaacttcc gatttcattt taggatattt gatccgagaa
     6421 tggaaggtac tgcttaaacc gctgatcaat gatttttcta ctttacggat ttcttttaac
     6481 gtcaatccac tgtcgtccaa ttgtccatcg gaaatacgct cttcaataag attatggaca
     6541 aaactttcga ttttttcgct cgttggatgg tccattgctc gtactgccgc ttcacaactg
     6601 tcagctatat tgacgattcc tgcttcacgg ctttgaggtt ttggtccagg ataacggaat
     6661 tgctcttccg tgacttccgg gttgcgttcc tttgccttaa cgtagaaata acgcatcagc
     6721 gtggttccat gatgctgtct gcaaatatcg atcaccattt gcggcatatt ataatcttcc
     6781 aatattttgg caccatctgt cacatgtcca aagataatat gtttactgtc ttctggcaat
     6841 aagaaattgt gcggattttc cgctcctgaa ggcaagttct caacgaaaaa gctggcgtgt
     6901 ttgatcttgc cgatatcatg ataatagcaa gctactcgag ttagtaaaga acgtccaccg
     6961 atttctgcta cagcattagc acttagattg gctaccatca tgctatggtg atacgtgcct
     7021 ggtgcttctt ccagcaactg tttcaataaa ggatgattag gattgctgag ttcatttaat
     7081 acgatcacac tatcatcatt gaccatcaat tcaatatagg ggtgcagacc tatcgtcagc
     7141 aagaatgaga acagtgcacc tgtgaatccg catactagcg ttaaccaagt cgttgcatcc
     7201 ccaaagctca ttccttggta gataatcaaa atgacatcca tcatcacagg aaaaataatg
     7261 acccacatag cagcggataa cccttgttct gagatccgtt tgcgtctgac gatcgttgcc
     7321 atgagaccag aaaatacata ggacatcaaa ataactgtca gaaagttcgt gccaatcgaa
     7381 tcataaaaaa tgaaaagtgc ggatactgct tgaaaaatac cagccatgat ccctgctctt
     7441 cgattcaaga aaaagcttaa aacaagtgga gcaaaagccg cggggtaaaa cagcggaata
     7501 taagcagctt gttctgtttg gaagagttgg aaaaatttca ttagtacgac gcttactgac
     7561 attgccgtca cgtagaaaag tacgtatttt gttctttctc ctgcattttg gaattgaagt
     7621 gaattataaa tcagtacacc gatttgaagc aaaacagcta aaaccatggc aatgagtggg
     7681 aaaatagaag tcgtttgatt cgtcaatcct agtaattcca attttttcat cgctgaagca
     7741 tcaatttgat ttccttcgcg aacaataatt tctccttgga agatcatcac tggttgtaca
     7801 gcttctcgtg cagattgttt taattcttct gtctttttct cattcaatac atcattaact
     7861 acaatgcctt gatccactaa gtagcgaatc atttgttgct gctctgttgt gacattcaaa
     7921 tattgaatct gatcttctgc ttcttgttta aaagcttcca attcgctttc acgaacttgt
     7981 ttactcatct gttcgtcaat gagcttcaaa ctttcatccc taaccgtgtg aatttccgtt
     8041 tcagtcattt caaatatcgt cgaataaaaa ttgttcggca gtttttggta aaacgcaacg
     8101 ttttctgcat tcacgttttc aaaatttttc ttcagtgtag caatccgctc ttctgtagta
     8161 ggtttcggga tattttcatc ttccttcgcc ttatctacct tgtcattata agatttgttg
     8221 atggtatcat tcgtcttatc gatcaattcg aataattgct tgatccgatt gttctggtca
     8281 tctgccagat ctttttgata agtatattct ggagtcactg cttctgctgc cagttttctt
     8341 ctttgttcgg tttcttccgt gttctcaatc gttttattcg cacggatact ctcttctgct
     8401 acttggcctt ctctgaaatc attggccttt tgtttgacac tactaaatgt aacgccgcag
     8461 acaatgataa aaaacaagaa cagaaggaaa ggcaatgctg cctttccgag ttttttttgt
     8521 agtttctgca gtgttttatt caacatgtgc ttatcctcct aagcaaagct tattcttgat
     8581 gaagatccgc cttttcataa gctttgatga tttcggcaac tacagggtgc cgaacaacgt
     8641 cacttgcttc aaaattaaca aaatcgattt ttttgatttg ctttaatgtt cgttctgcat
     8701 gaacaagacc acttgtcgtc ccacgaggca agtctatctg acttgtatcc ccattgacaa
     8761 tcatttttga ttgataaccg agacgggtca agaacatttt catctgagcg actgtcgtgt
     8821 tttgtgcttc gtccaagata acgaatgcat catccaatgt ccgcccccgc atgtaagcta
     8881 aaggtgcgat ttcgatcacg cctcgttcca tcaatcggtt cgtatgatcc aatccaaaaa
     8941 tttggtataa agcatcataa acaggccgca gataaggatc cactttttct ttcagatccc
     9001 cgggtagaaa gcctaggttt tcccctgcct ccacagcggg ccgcgtcaaa atgatttttt
     9061 gcacttcgcc tttttttagg gcagcaattg ctaatactac ggcaagaaac gttttccctg
     9121 ttccggctgg ccctacacca aatacgatgt catgtttggc taccgattca acgtatttct
     9181 tttgaccgct gttttttacc cgtataggtt ttccattacg gtctttgacg atctcttcct
     9241 catacatctc aacaaaataa tctaatgtac ctttttgccc catcttcaat gccgtcacaa
     9301 catctggcgt acttacgtgg attcctcgtt tgatcaattc ctgtaatgtc cgaatgactg
     9361 aggcagccaa ggaaatctgg gattgctcac caatgatctg aatgatttct ccacgtgtat
     9421 ggatggttgt ttccgttgtt tcctcgatca gttttatatg cttgtcgtgt gcacctaaaa
     9481 gcatgctgac atcatcagca gctgtcaatc taatctctaa agaactagac tcttccgtca
     9541 aaaatagaca tccccttttt ttcgattcat tctttcctat tttaccatac tcagaagaaa
     9601 ctatatagca aataagaaaa ggttgtaaaa gagtcgttta accatacgta ccaagaaacg
     9661 ttaaaaaata gccgtttcca ttcgctaaag gtctcgtttt gataaaaaga agctgagaat
     9721 gtaagttata tgaaagtgga gaacaaaata ttccgtttca ttcagcaggt attctctagg
     9781 tatttttctt gtttcaaaaa aagaaaacaa aatagttaaa atattcttgc atttcaaaaa
     9841 aaacaggtgt attattacgt caacaaataa acatacagca aagcgtcgag gaaaacagta
     9901 ggctattcaa cgatttattc ccagtgagtc atgtaagtga gaaatgacaa taaatcgaat
     9961 agacgaacat catttccgag tcaatgtcga aaacgtagat cattgtggct gcctccatta
    10021 tcaggctagc ggataacttt atcaaaaagc gtccgtgaat gaagaagctt ttttaagcga
    10081 aaaaaggtgg taccgttcta gaatctagag cccttttccc aagtggaaaa gggctttttt
    10141 tatattttaa ggtggtggtt ttcatggaat ctgatattag tggcattact aattgctgca
    10201 acgtttatat cgtcaatcaa aatgataatt attggaggaa aaatttatgg aagaaacaaa
    10261 aacttattca tggaaacaac tattgatggt cagttcactt atcttcggga tgttcttcgg
    10321 gtcaggtaat ctaatctttc ctgtccactt aggtcaaatg gctggcgcaa actgggtcag
    10381 tgctggcgta ggatttgcca tctcgggcgc tcttttccca ttactagcta tcttagctgt
    10441 agtagtgaca aaaagtgatg gtctgtatga cttagctaaa cctgtcggaa aatggtatgc
    10501 tgccttgttc ttaatccttg ttcatttgac gatcggacca tttttcggta caccacggac
    10561 agctgctact gcttttgaaa tggctgctaa accatttttg cctgaaaaat ataattcatt
    10621 aggtatgttt ttattctctg ctcttttctt tttacttgct tatttcctta ctgtccatcc
    10681 cacacggttg gtcaaatacg tcggaaagta tctaaactcc ttgtttcttg cattgttagc
    10741 aatcattttt gtcgtttctt tattccaccc aatgggaaat ctaaatcaat ctgctgcaga
    10801 tacttaccaa acaaacgctt taacaaatgg tgtcctagaa ggttataaca cggttgatgc
    10861 agtagccttg cttgccttaa gtgtcacttt tgtccatgca gtccgtgacc ttggtttcaa
    10921 agataaaaaa gtcacagaat taacagcaaa agctggtagc ctgagcgttg cgctagaagt
    10981 catgatctat tttggtcttg ttcttctcgg tgcgatgagt ttgaataaga tgtctttatc
    11041 agaaaatggc ggaactgcac tttctcagat cactaatttt tatttaggta acttcggcgc
    11101 tagcttctta ggagtgatgg tcacattagg tgtcttcact accgctatgg gactcgttgt
    11161 ttcttttgcg caagatttcc ataaactgtt ccctaaagtc agttatatga catggctgcg
    11221 gttaacgact ttcgtctctt ttgtcgttgc aaatgctggt ttagacaata tcatccaatg
    11281 gtctttacca gtattgatgc ttttgtatcc attgtcgctt gcgttgatct tgctttcctt
    11341 aacagcaaaa tttttccaaa agacaccatt tgtctatcaa gtaacgatgt tgtttgctgc
    11401 tgttcctgcc gtattggata tgctggcaaa ttcgcctgct ttagtgagtc aacaaagagt
    11461 tgtagcaagc atgctcgaat tttatcatca tcacgttcct tttgctgcat taggacttgg
    11521 ctggatggtc ccaacgttac ttggttacgc tggcagccta ctgttttatt acgcctatcg
    11581 cctatcaggg tataaacaag aagcaaatga actaccagaa gaataaccaa atcaaacaca
    11641 atttaacata agaaatatta aacaggaaaa aaccagctag atgaaagaat cctattcttt
    11701 cgtctagcta tttttttgtt ctctttttta cgcgcacaaa aa
//
LOCUS       DAYYGS010000081        11719 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000081 DAYYGS010000000
VERSION     DAYYGS010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.081"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..276
                     /locus_tag="ACHA44_002216"
     CDS             <1..276
                     /locus_tag="ACHA44_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFM8905475.1"
                     /translation="PSIASAFKKLKEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKF
                     YRSLRTASPTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(891..1931)
                     /locus_tag="ACHA44_002217"
     CDS             complement(891..1931)
                     /locus_tag="ACHA44_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8905476.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2280..2606)
                     /locus_tag="ACHA44_002218"
     CDS             complement(2280..2606)
                     /locus_tag="ACHA44_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905477.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2593..3381)
                     /locus_tag="ACHA44_002219"
     CDS             complement(2593..3381)
                     /locus_tag="ACHA44_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8905478.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3749..4039)
                     /locus_tag="ACHA44_002220"
     CDS             complement(3749..4039)
                     /locus_tag="ACHA44_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HFM8905479.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4175..4354)
                     /locus_tag="ACHA44_002221"
     CDS             complement(4175..4354)
                     /locus_tag="ACHA44_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905480.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4351..4701)
                     /locus_tag="ACHA44_002222"
     CDS             complement(4351..4701)
                     /locus_tag="ACHA44_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905481.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4694..6019)
                     /locus_tag="ACHA44_002223"
     CDS             complement(4694..6019)
                     /locus_tag="ACHA44_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFM8905482.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6469..7083
                     /locus_tag="ACHA44_002224"
     CDS             6469..7083
                     /locus_tag="ACHA44_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905483.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(7248..7490)
                     /locus_tag="ACHA44_002225"
                     /pseudo
     CDS             complement(7248..7490)
                     /locus_tag="ACHA44_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7694..9013)
                     /locus_tag="ACHA44_002226"
     CDS             complement(7694..9013)
                     /locus_tag="ACHA44_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HFM8905484.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(9147..9755)
                     /locus_tag="ACHA44_002227"
     CDS             complement(9147..9755)
                     /locus_tag="ACHA44_002227"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905485.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSLNNSYF"
     gene            complement(10393..10650)
                     /locus_tag="ACHA44_002228"
     CDS             complement(10393..10650)
                     /locus_tag="ACHA44_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8905486.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10643..10912)
                     /locus_tag="ACHA44_002229"
     CDS             complement(10643..10912)
                     /locus_tag="ACHA44_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8905487.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(11043..11222)
                     /locus_tag="ACHA44_002230"
     CDS             complement(11043..11222)
                     /locus_tag="ACHA44_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905488.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            11320..11556
                     /locus_tag="ACHA44_002231"
     CDS             11320..11556
                     /locus_tag="ACHA44_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905489.1"
                     /translation="MKQNTSIAKNTTKFKCTAAAWRISISPTNRTNNRGMILFIWISF
                     FLLGYRVLPAYFAPPKRRGRLERLVRNKNHSLSY"
BASE COUNT         3609 a         2123 c         1781 g         4206 t
ORIGIN      
        1 ccatcaattg ctagtgcctt taagaaacta aaagaatacg gattttatca agggacagaa
       61 catcgtacca ttaaatacct gaataatttg attgaacaag accatcgtcc agtaaagaga
      121 cgcaataaat tctatcgaag tttacgcact gcctcaccca cgattaaagg catggaagcc
      181 atccgaggat tatataagaa aacccgaaaa gaaggcactc tcttcgggtt ttcggtctgt
      241 actgaaatca aggtattatt gggaatccca gattaaatca tagataccgt aagggatttt
      301 attctttatt taaaactttg caacagaacc gtccgttgcg ggtaataccg taacactgaa
      361 gggcacgcat taaggcgtca tcttaatgct ttcctgtagg acactgacgg agattaccag
      421 tctcccgagc cttacagggg cctttctttt tttgtatttt caattatcac aatttatcgt
      481 ccaaaagtgc agaacgaaaa tcaattcatc acaagcatat cagacaatta aatttaaaac
      541 aactaaaaat atacaaaaaa gtgtaattga atattagcag cactgcctga aaagaagtaa
      601 tatcaggctt ttcagcttgg ggaaataagt gaattacctc tcttcatgaa ttattataac
      661 tcaaaaaagc acatatgcaa acactagttc cctatttttt tgaatgaaaa ataactaaaa
      721 tatctaacga ttatgacttt aaaacctacg tttgcgattg ttttcataaa cttttaatct
      781 aaaaactccg gaataatagc tatttatttg taacatttgt aataaaatta aaatcataaa
      841 aaagttctaa tttaaaattg atcattttta tagtgatcgc tttttatcaa ctactctagc
      901 caattttccg tactaactgt tggtaattcg ttaaatgttt ctctttgttc gtttgcaagc
      961 tttgccgcaa tagctttttc gacaaaccaa ttttttacat aaacgaaaat tagattctgc
     1021 aaattgttta ctttttcagt tttttgtttt tgataagctt tcagcaaagt catatataat
     1081 cctttgtcag catcaattcc ataagattcc agttcttcat aaacaacaac tgtttgctct
     1141 aattcttcag cttttttctt tgcattatga atagtttgga cggttttttg agcgtcttcg
     1201 taagtactag aaaatgtttt tatcaagttt aatacatttt ctggtacaaa tgtatttata
     1261 ctgtcctctt tcatgatgtc gacgaagtta tctagtaaaa cttgattttg taactgatca
     1321 gtttcagtgt ctattatgtg tctattagtg tctatagaat tagaatagaa tagattgtct
     1381 gcgattttcg caattacggt tgagtccttg atatgactgg attttgccgt atttgcgatt
     1441 ttcgcaatta cggtggtatc attgatttct cgcggttttg ccgtatttgc gattttcgca
     1501 attacggtgg tatcagtatt gtcttcctct tccatgattt taaagacgtc ttgattcgtt
     1561 acttctggct tgcctaagta caataaatta ggcatggatt caatcttacc gtcacgttta
     1621 tttactcttc ctctcttctg tttcagaaga cctgcttttt ctaattcctt tttgatttta
     1681 gatactttac cttcgcggca ttgtaacaat ttcattaatt cttcaactgt gaatatgaaa
     1741 aatatattat catcagaatc tacccagtta tttttgactg aataatcaaa tcgatcttta
     1801 agtagcatat aagcaatttt tgcatcattt gatagatcca tgtatttttc gttagtaaaa
     1861 aataccttag gcatttgata atatttttca cgataaattt cttttgattt gtaataatta
     1921 aaatcattca tagccaatcc tctttctgta ggatagcgtt ttgttcaaaa ctatgttaca
     1981 atgaattaaa caaatatatc tatccactat atatttttct actgctgata agtcgccaaa
     2041 ctttgttaaa gcagtagtct ttttttatct ttttttagtt taccttaaaa aataaattaa
     2101 agcaaaccgc tctatttcaa ggtttaagct catagtaact atatcacgat attacaataa
     2161 tattatatca cgatatcgta atattacaat atgaagataa gcgacttatc agctttgata
     2221 ctactgattt ttataaaaaa aatatcacga tattatgata tcgtgatatt gaatgtaaat
     2281 taaattttat tttgtttctt tgctagttta tatccttcaa tcaagtattc ttcaggtatg
     2341 gactttaata gcgcatcttc tataacgtct ggtttacttt tgtctgattc agcaatagtc
     2401 gtccaatatt tgagtgctga cattagaatt ggatttatac gaatgctatc tgctgttctt
     2461 acactacttc cttgatcttt tatgacttta cttatatttt tcttggttaa ttcgatttct
     2521 tctacttctt ttgatttaaa attcttttta tttttatttc ctagtaatct acttactgca
     2581 gcttgactgc gattatcttc tgccatcaaa atcaccaact tcttttaaat attcttctgt
     2641 aaatgcttta tagtcatctt cagctgttga gggtttatta aaattgatta gatctgatcc
     2701 agcagcttgc gcttcgtcaa cggttattgt cctacgaata taagttttga aaatttttgc
     2761 accaatgact ctagaaattt cggttgctgc agacgtcatt tctttagaaa tgttataacg
     2821 cttattatac ttagtaaata gaactcctcg tacttcaagc ttgtcgttcg tatattcttt
     2881 taccgtttca atagtttcac ttaactttat tagtcctttt aatgagaaat acgcagcttc
     2941 aactggaatg ataatttcat cagaaacagt aaatgcgttg actgtaagca ttcctaaact
     3001 tggtggacaa tcaattataa tataatcata gttgtccttg attggttgaa gtactctttt
     3061 aagcctgtgt tcacttccta cttgagtaaa ttccctctct gcagaagaaa gggctatgtc
     3121 ggctggcaaa acatcaattc ctttatagtc cagaatagca tctgtaatgt cagcctcccc
     3181 ttttaatact tcataaattg taggttggtt tgttccatct atatcgaaac ctacattatc
     3241 tgtagcattt ccctgaggat caatgtccac taaaagtaca tttttaccgt aaacaaattt
     3301 caaacatgct gctaaattaa cgctagtcgt agttttagaa actccacctt tttgattggc
     3361 tactgacaat attcttgcca ttaaaatatc ccccattcat cttatattat aatataatta
     3421 tatcataata ttgtgatatt gcaatatcgt atatgcatta tattgtaata ttgtgtgatt
     3481 attttgtttt aaagatatat atcggttcgg atataaagat tattcatatg ttattcgaaa
     3541 ttaaaataat cactttttat tactttaaaa ttttttatta atgataagaa atatgttttg
     3601 cgagcagcaa ctagaataat ttaaacatta ctaaactgtc tatattgtta ctaataattc
     3661 ccttcttata gtgcattttc ttagaaaaaa acaatattac gatatcatga tattaagata
     3721 tcgtaatatt atacaattgt gattgatatt atatatgttt ttttattttt ttatcatgtt
     3781 taatcttgaa ggaatcatat tctttttcta aagacaggag cttttcataa acatcagaca
     3841 tgtttaaact agaggccagg cttaaatcat aaacgaatga agcaggtaat gattttattc
     3901 tacgttgtcg ggttatccag cttgcaaccg ttccttgtgg gtaggcatat aactcacaaa
     3961 attgttctac agtgattcct aaattcttga taatgtaaac attgatcggg tgcgagtaaa
     4021 caaatgtatt gatagacatg gtgcgtaagc ctactttctt ttattattgc gtttgcaata
     4081 atagtttaac acacgcatta ttctagtatc tactcctatt tgtaaaaaca aaaaaggatt
     4141 catagagata actctataga atccgtaaaa taccttatat atataacggg aagatataaa
     4201 acctaaaatt atgataacct gctgcagctt ctacatagaa agagaagcgc tcaacaccaa
     4261 cctcatttat caaacgttgg ccataacccc aaatctcttc tgatagctga tcaaaggtta
     4321 tatactttag ctcatattgc tggataatgt tcattctaat cgactccatt tattatcctg
     4381 gtaaaattcg atatgacgta tttcgtcgta gtgaattttg tcttttccaa cgtagatccc
     4441 cagctcatca ctacctaaga tcttgccaat gatatcttct ggatagtatc cttcagcgtt
     4501 tcgttcttct ttttgaatag atactgatct attttttatg actgcagcgt tcaatacttc
     4561 cgcaatctct tcagtcgtca tttgttcttt tgctaaattt tgactggttc gttgttcatt
     4621 gtcggcatca atttcttcag tatgatcaga taaataaaac cctgcccact ttaaacgtcc
     4681 acgatcattg taatcaatca tttttaattc ccccatttcc gcccgcatga ccaccaacaa
     4741 gtgtgcttct agtaatgctt ctagcgcctt ctaacattga agaagcatgt acaatcgacg
     4801 taaaaccata tttttgccga atcttgtcaa taattttatc taaattttct tcgtcaattt
     4861 gcttttgtgg atcactaaat aaatccagct gaagcgaatc ggtgtacaca agcttgctat
     4921 aggttattcc aacatgcctt acttcttgtc cttcataata cttatcaaac aagaaaagac
     4981 aataattctg caattccttt gtattatctg tgatagggat cttcatctgg tgtgaaaacc
     5041 ctggtctcgt ttctaagatc gacgtgccaa tgtgtaaatg gacacaacct gttttacagt
     5101 tatgctgcct aatgcgtatc gctacttgtt ccgccatttc ttttaccacc agctcgattt
     5161 catttcttct agtataatct cgtggtaaca cttgtgaatt tccatatgat ttttcctttg
     5221 tcggttctgg tgggattgct atatctgttc gatcgatccc attagcatgg aagtacagct
     5281 gtaagccaat cacacctagt ctattcttaa tcgtataagg attccagtta gcaagatctc
     5341 taatgctcat aatccccatc tgattgagtc tttttttcat tcgagagcca attccccaaa
     5401 agtccgtcat ttcaggaata ttccatactt tttcaggaac gttttcgtac gtccattccg
     5461 caacaaatcc ttcattatgc tttgcttcgt tgtctaaggc tagtttagcc aaaaggggat
     5521 tatctccgac acctactgta gcaatcaatc ctagctcttc tttaatattc tcctggatca
     5581 tttgagccag cttctttctc cgttgacttc gtgttccttc agtcgtaaaa agattcagtg
     5641 atcgggtcac ttttaagatt gattcatcga tcgagtaaat cagtagatct tcatcagcca
     5701 catatcttct gaaaatatta tttacctgca tatttcgctt gatatatagg ttcatacgtg
     5761 gtggaacaac gtgtagtgtt ttaggaaatg gttgtggtaa gtcacgtggt ctactcacat
     5821 ttgtaatacc ataccgcttt tttgcttcag gagaagaagc taatatcaat cctgaaccag
     5881 tattatcagc tcgactcata acaacaagtt ctgttgttaa tggatctaaa tttctttcta
     5941 tacattcgac acttgcgtaa aagcttttca tatcgattaa aaagtaatca tttactggct
     6001 cttttgaata atccatcata aataacactc cattctttca cattatacaa acatatgttc
     6061 gatattgtaa agaagaaata gctaaataca taaaaaaaga agcataggta tgctatcacc
     6121 ctgcttcttc ttttgatttg tatgatcaat ttattctatc actttactaa ttaatttgaa
     6181 aatctaataa aaactctcac tctaaaagag tgagagactg caaattagag tacggacttt
     6241 tttggagttc cgttctttaa tattaacaaa tgaataaaat ccttacaaca tttgagaata
     6301 agttatcagt agacacttac aaaaaattct catactattt cctccatcat tagtttttgt
     6361 ctatataagg tttaccctaa atagacactg gtataacatt gaaaaatatg gcttcctcgt
     6421 gtatgattag ggtgtatttt aaatagttgc agaaagtagg taaaagtttt gagagttgca
     6481 tacatacgtg tctcatcgat cgatcaaaat gaacaacgcc agattgaaga aatgaaaaaa
     6541 ttcggtgcag agagaatttt catcgagaaa caatctggcg caacgattac tcatcgacct
     6601 gttttccaag aagcattaga ttacgtccga gatcaagata tttttattgt ggaagctatt
     6661 gatcgacttg gaagaaatta tgacgagatc atcgcttcag tcaattactt gaagaagaaa
     6721 aatgtgaagc tgatcattac tagtttaccg atcatggctg aagcaattgg caatcctctc
     6781 ctggataaat tcattaagga tctgatcatc caaattttgg ccatgatcgc tgaacaagaa
     6841 cggactgaat cgaaacgtcg ccaggcacaa ggaattaaga tcgcaaaagc aaacggcgtc
     6901 tacaaagggc ggcccaaatt atacagtgca gacgcaaaag atccccaacg tcgccttgtt
     6961 tacaaaagca tcgttgaaga tcttaaaaat ggtgttgcga ttgctaagat tgctaaagac
     7021 tacaatgtta cgagacaaac cgtgtataga ataaaaaaag acagcatggt caatgacaaa
     7081 tagttgatca tgctgctttt attatcttct catctttaat ttcctacata taattactat
     7141 ctataaaatt aagtcttact cagaacagat aaagcataaa attccaacaa accatttttt
     7201 acttaactaa ataaagagtc tgttaatata atcttcactt atttacgtta atcccctact
     7261 gtatttgttg gttccttatc tatattttct acaaaatcct tattatcttg tgatttagct
     7321 tcgtataatt ctttgtatgt cgacacattg gttatactaa ttcctatagc tagttcttga
     7381 gactctactt ttctctctag ctcctttgac aaagtgattt gagatttatt tggttccgtt
     7441 ggagtcaatt ctatagattc ttcataaaaa tctctataca taaagctatc cctgaataat
     7501 tcatagcttt aattctttgg cacattttat tgaaatatgt atcactaata tccctatcaa
     7561 ctgattttga ctaaaaagtt acttcaaaca ttcactgttc aaaaatattg aggaataaat
     7621 tttgggtgta aggcattttg agcatacttc tccctggata acttcccagt aaaaaaatcg
     7681 ttagattgtt ggattagatc cattgactgg ctcgcctcag gcgagcaaag accctgtaga
     7741 attcattctg atacacatga taactagata atttgagata gactcgtcta cccgtttgga
     7801 ctaatttccc agcaactttc agaaacttca atcgaataga atgaatggtc attccctttt
     7861 ggacttcttc aaagccaatc gttcttaaga agttgactaa gttgtaagct atcaagctga
     7921 gcatcattcg gacatgattc tccaaaaagc gaggactgtc tgttttgtca aagtaaaagc
     7981 cagctttcgc ttctttaatg aagttctcca ttgtgccacg tttggcatag atagaaaaga
     8041 tggtatcagg agaaacattt tctgatagat tcgtcacgat aaattcatgt cgaaagagta
     8101 gttcgcccgc ttcacgtgtt gagcgtatac acacgcgacg actttgtgac caggattgtg
     8161 cttgataagt ggtcgagaag tactgaactt ctcgttcttc ccacttttga ttatcgccat
     8221 aaagtactga tttctcagct atttgaccta gtctacgatt attcttcagt cgaataacat
     8281 aatgactctt ttttgattca cacgaatcat acacaccagg tgtagcgaac ccgctgtctc
     8341 cacgaaccaa gatgtcagtg tttggtagag agtgattata gtgctctaat aaaggtgtga
     8401 gaaactcctt tacgcctttt gatgtatatt gatttcctga acgtagttca gcttttaaga
     8461 agtcaccagt caatccgtca aaagctacca atggatggta accataggtt tggtagtgtg
     8521 tattataatt cgtttgttct tggtgaccaa atgtatctga atgggtcgaa tctaaatcaa
     8581 tgattaattc cgtatcatta cggatgaggc gtgctttatc gataagttct tggttcaaag
     8641 cttgaagttc atggatattc tcctcagata gtcgatctaa aaatcgggaa agtgaagatt
     8701 gagaagcgag ttctttccta tctaaaacag ctttaaacac aggatcttgt ctcaggagat
     8761 tagctgcaga atcagctgag taaccagcaa ttaattgcat aatgaactgc tcaagtatgg
     8821 ataagttgtc atgagtaaaa tacgctcgtt cgtcttcaat gtgaatgtgt tgcttagcca
     8881 gttcagaaaa accaaagatg ttcatcagct ctttcactag gacgagaccc gaatcactcg
     8941 ataattgacc acctgtatga gaaatagtga tatttgaatt gaattttact tggtttttgt
     9001 gtaagctagt catgaaaaga actcctttct tttggttggt ttagtcactt taaccatagc
     9061 agaaaggggt tcttttttca tcacttaacg ggtgaagatg aaaaagtaat tgtaagctgc
     9121 cgtgaggcag tttcacatgg ttttaattaa aagtatgaat tattcaggct atcattaatt
     9181 ttataaaaaa aattaaatgt ttttagcact gccatataac attcaaaaca ttcttctata
     9241 gttggtttac ttccttgagg agaatgaacc aatttattac gtttttctat gaaattttta
     9301 atttcctttc ttatttcttt cggaaactca atattaaagt accctttaaa catgctccct
     9361 aataatttgt ctacaggggg agattgtaaa ttctcaaata ttagagttaa atcaggtttc
     9421 tcttgttgat aaaattcctt tacccctatt tcacaggctg taattatcga aataaaccta
     9481 tctctagtat ttgatatatt tttacttctt ttaaggagta taagagaaaa ggggatactt
     9541 tcgtgtccat gcatgtaatt aactaattgt tgagcttcag aagttatatc tctaattata
     9601 tagttaacgg aattaggaag tgtctttaac tctaatgaaa tttctatcca attactatct
     9661 tctaatctgt atatgctcga agtataattt gattcgtcag caagcatcgc tacatcccaa
     9721 acactgatca taaaatcttt taaacgttca atcatttcag caatattgct aaaatcagct
     9781 aatttttctt tagaataaaa aaaaacattt atatcagaat caataaattc tattttttta
     9841 ttactatatc gtcatcatta tcttgaccat ttatgtttat tgttccctga gtggggagta
     9901 cttctttcaa attcaagtta aatttatatt ccattacttc tcctccaata tataaaaatt
     9961 acttaactgt atccaaattt aatatgtaaa taaatcatta atttttagct aaattctatg
    10021 agaagtttat ttttgttgtt tttgctaatt aactaaaact agtacaaaaa atttaaaata
    10081 gattattaaa cctattgctt attctattca gtcatatata taagcgtcat cctttagtaa
    10141 ctaacatccc acactacaat agtattataa catatctatt caacttcaaa ttgttgaaat
    10201 ttaagccatt tatatttact tttcctcaac tttagaaatg attttactaa ccttatatcc
    10261 gtaatacgcg taacaaacac attcattaat aacaatcaac actaagctat attagctttt
    10321 taaatgacta gggtaaataa aataaggatc aaaaactttt aagtttctga ccctttcctt
    10381 acttccgatt ggttaatagt gatcttttgc agaataaata aatatcgttt cattttcaac
    10441 tctatatatc agtctatgtt catctgtaat tcttctggac cattttccag ataaatcatg
    10501 ctttaatggc tcaggttttc ctaatccagc aaagggggaa cgatcgatat cttttattaa
    10561 cttgttaatc ttttttatat tgcttttgtt tccttgctca tgccaataaa gataatcatc
    10621 ccaagcatca tcagaccaag ccttaatcat cagattcaac ctcgattaag tcatgtgttt
    10681 taaatgcacc tttggagaat tgttcatctc ctcgacgaat tttttccatg acgtaattat
    10741 tagaaagtgt tctcaacgtt tcttgcatag aatcataatc tctttttgat aatacaacaa
    10801 ctgtatcttc tacatcttta cttgttacaa taagtgtttc agcatcctca ttaacttgtt
    10861 tcatataact acgtaaattt tggcggaaat ttgaataagc tactgcttcc attcctttca
    10921 ccccttattt atttctataa aacaattgta cattatattg tacaattaag caaatgatta
    10981 atattctaac ttcttataat taaaagtgct atcactttta gtttttagga taaaaagtga
    11041 cgtcactttt tgagtgctgg tacctcaaat cctgcttttt cttgggcaat caataaatct
    11101 aacgcaatac acataatcct ggattcactt ttgccgtact tctgctttag ttgctgcaat
    11161 tctttttgtc tcttttcatc caacgtaacg ggtaaccgtt tatttcctat tgcaattggc
    11221 attagtactc tctcctttca tcgaaataac cctatgaaaa atatggctgc aatcatcttg
    11281 aatagtagac tcacaagggc taatacaaag cgccaaaaga tcaaacaaaa taccagtatt
    11341 gccaaaaata ctacgaagtt taaatgcaca gctgcagcat ggcgcataag tatttcgcca
    11401 acaaatagga ctaacaatag aggaatgatt ttattcatat ggatctcctt ctttctctta
    11461 ggttatcggg tgttgcccgc ttatttcgca ccacctaagc ggcgggggcg gttggagcga
    11521 ttggtgcgaa ataagaacca ttcccttagt tactaggttt tttgtcctta tttcacacat
    11581 tctcactttt cggtataaat gattattgcc tttttttcct ttcaaatcgt tgatatggca
    11641 ttcgtttacg gtcaagttca ttttggattt cttctgtatc gtcgtaagta taggtgtcta
    11701 aaaaatcagt taaagtcgt
//
LOCUS       DAYYGS010000082        11603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000082 DAYYGS010000000
VERSION     DAYYGS010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.082"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..305
                     /locus_tag="ACHA44_002232"
     CDS             6..305
                     /locus_tag="ACHA44_002232"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905490.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="ACHA44_002233"
     CDS             378..1739
                     /locus_tag="ACHA44_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFM8905491.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="ACHA44_002234"
     CDS             1761..2021
                     /locus_tag="ACHA44_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905492.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="ACHA44_002235"
     CDS             2035..2787
                     /locus_tag="ACHA44_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HFM8905493.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGKKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="ACHA44_002236"
     CDS             complement(2881..3486)
                     /locus_tag="ACHA44_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8905494.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="ACHA44_002237"
     CDS             complement(3539..4513)
                     /locus_tag="ACHA44_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HFM8905495.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="ACHA44_002238"
     CDS             complement(4891..5610)
                     /locus_tag="ACHA44_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905496.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA44_002239"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="ACHA44_002239"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0019191 - cellobiose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8905497.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="ACHA44_002240"
     CDS             7161..8558
                     /locus_tag="ACHA44_002240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8905498.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="ACHA44_002241"
     CDS             8639..9079
                     /locus_tag="ACHA44_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8905499.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="ACHA44_002242"
     CDS             9227..9553
                     /locus_tag="ACHA44_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8905500.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="ACHA44_002243"
     CDS             9554..10303
                     /locus_tag="ACHA44_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HFM8905501.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="ACHA44_002244"
     CDS             complement(10383..10838)
                     /locus_tag="ACHA44_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8905502.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="ACHA44_002245"
     CDS             complement(11265..11540)
                     /locus_tag="ACHA44_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905503.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3663 a         2014 c         2356 g         3570 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtaaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa aca
//
LOCUS       DAYYGS010000083        11525 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000083 DAYYGS010000000
VERSION     DAYYGS010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.083"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            332..538
                     /locus_tag="ACHA44_002246"
     CDS             332..538
                     /locus_tag="ACHA44_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HFM8905504.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(740..1741)
                     /locus_tag="ACHA44_002247"
     CDS             complement(740..1741)
                     /locus_tag="ACHA44_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8905505.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1746..3659)
                     /locus_tag="ACHA44_002248"
     CDS             complement(1746..3659)
                     /locus_tag="ACHA44_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8905506.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3827..4333)
                     /locus_tag="ACHA44_002249"
     CDS             complement(3827..4333)
                     /locus_tag="ACHA44_002249"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFM8905507.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4493..4933
                     /locus_tag="ACHA44_002250"
     CDS             4493..4933
                     /locus_tag="ACHA44_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8905508.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(4959..5987)
                     /locus_tag="ACHA44_002251"
     CDS             complement(4959..5987)
                     /locus_tag="ACHA44_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905509.1"
                     /translation="MYWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWIS
                     LLRISLQRMKQGHSITYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTE
                     QAYLYFMFGVMNTYLEEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLY
                     VNLAMIHLYSRVFGTKKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNT
                     YYEKNRRLIFQYCMLVRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYS
                     ASADQLPDIVISDYPIDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSI
                     LEKLDFVN"
     gene            complement(6042..6116)
                     /locus_tag="ACHA44_002252"
                     /pseudo
     CDS             complement(6042..6116)
                     /locus_tag="ACHA44_002252"
                     /inference="COORDINATES: protein motif:HMM:NF016901.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6119..6475)
                     /locus_tag="ACHA44_002253"
     CDS             complement(6119..6475)
                     /locus_tag="ACHA44_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905510.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(6773..7747)
                     /gene="sgrA"
                     /locus_tag="ACHA44_002254"
     CDS             complement(6773..7747)
                     /gene="sgrA"
                     /locus_tag="ACHA44_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HFM8905511.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(7943..8782)
                     /locus_tag="ACHA44_002255"
     CDS             complement(7943..8782)
                     /locus_tag="ACHA44_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HFM8905512.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            8967..9854
                     /locus_tag="ACHA44_002256"
     CDS             8967..9854
                     /locus_tag="ACHA44_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFM8905513.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(10448..11142)
                     /locus_tag="ACHA44_002257"
                     /pseudo
     CDS             complement(10448..11142)
                     /locus_tag="ACHA44_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(11126..11524)
                     /locus_tag="ACHA44_002258"
     CDS             complement(11126..11524)
                     /locus_tag="ACHA44_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFM8905514.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3437 a         2295 c         1726 g         4067 t
ORIGIN      
        1 tcattttagg ttatcatttg taattttatt tcacagttga tatgattgtt atgtaaaata
       61 aaaaagttta ggatacatcg aatttttggg gaaattcttt tgggggtatc ctaaatcttt
      121 tctattatat tcccatattt tctattattg gtctagacgt atgatggtaa actttgtacc
      181 tatctgattg ctgaatcatt aattgcttat cattagccat ctttgtttca agttcgctag
      241 atgatcattt tattcacatc ctaacaaact tgtcacattt atagattctt aatctattta
      301 tcatacaatt ataaaaaagg aggtgctgct tatgaaagct atccatattt ctttgctttt
      361 tttcgctagt gcattgattt tatttgctat tcatctttgt tcccagcctt atatcaataa
      421 caacggaata ttagtggaac cagcattctt ttgcttacca ctcggctttt tgtcccttct
      481 tgcctccgca ggcttcggat ttgttgcttt ttttaaacga tcaaaacaac aaatctgact
      541 tataaaattt acgtaaaaga gatcgtgaca ataatcgtgt gatattccca aatttcgaat
      601 aaaaggtgcc ccttgccatc tctaactcct cagtcacaag ttacaatatt cataaaccat
      661 gaaaagcttg tttattgaaa ttgctccttg tgacttggag ctaagtgaca gattacaacc
      721 tcttttgcat cataagactt tactccttga aaaacattac taacttttga tatgtttctt
      781 gcgcttgttt attgctgaaa tcaaactgat attcatggtt caaaacagca tttttctccg
      841 cataatctac ttttgtgact ttcacccccg ctttctcaaa tacagaagtt acttcttcca
      901 tttgtttcgt aaacgtattt gtatttccat ccgttaagaa tgtgttttgc gtccaacgat
      961 ccgcatgtct gaccaaggct aaacttttga ccttctctga ttgttcaaag tccttattat
     1021 caaagtaagc ccatccacat ttttcataaa gaaagctaga gattttatca cttacttgag
     1081 taagtgcaga aggatccaac agaccagaca aagaaataaa cttcgtaatc tgcttgttct
     1141 ccaaagtcgg atcaagatga tcagcttttt taatttcttc attttgaagt gctaacaaat
     1201 attctccact aatttgtgca cctgccgaat ttccaccaat cgaaatattc gaccaatctg
     1261 cttgatcttt tatctctttt ttagctgctt gaatggcttc gttcaattgg ataactggtg
     1321 taggatgttt tgcttccggt gatcgttcat aatctatatt tacgatgatt tgctgcgtgt
     1381 ctgaacatag atattccaaa taatcacgac aatcattttt atcccctcct acatatgcgc
     1441 ctccatgcat ccagaaaaga atcggtaatt ttttcttttc cactttcggt gtgtagatat
     1501 ccatattatt atttttgtac tttgaaggat aggatatttc tttttttata tcgatttgtt
     1561 cgattttttg ctggaaatca tctggtcggg catatacttg ttctttttca cttgtgtcaa
     1621 attgttttct aacaagcaag ctaatcggct ttggtgtgat ttgattaatc aaaaatacag
     1681 tgactaaaat aactacgatg ataactggta ctgcaatcac tattcgcttg aaccatttca
     1741 ttcatttacc ctctctttct tcgccaaatc catgtgataa tcgtgctaac agataaaaat
     1801 ccacctgaat aaatgagaag cgaatgtttc ttttctccag ttttcggcaa cattcttttt
     1861 tttgcatctg tctttttttg tactttcact gtcatctgaa tcggttgcgt atcatcactt
     1921 gattgatcct ctcgaggttc attctttccc ttattgtctg cattctctgt cggatcttgc
     1981 ttttttggtg gagctactgg gatttttttg taatagaaaa taatagttgg tatcgtctca
     2041 ttaaaaatcc cttcagtcgg tccttcaaca tttgctaatt cgtatccttc gaatgtcttt
     2101 tgctctgtca tataattttc tccataagct ccgtaaagct ggatttcgtc ctggtctttg
     2161 agtagacttt tattgttttc atccacatac tttacgttta tctgatcatg gaacactact
     2221 ttcaaaaagt ccgttacatg tttcgtgtag gcagcatggt caacttcctg tgcctttcca
     2281 tgccctgctc cttctactaa taaaagatct ttataccctc ctgataactc atataatttt
     2341 tgcgcgttag acacaggtac tacatcatct gctgttccat ggataatcaa tttaggcaat
     2401 gaatctgaca ttccaatcgt aggtagagca gcttttgtat ccaaatgcat ctcctgatca
     2461 aaatagtatt gtttcaaaag gcccataaat aaatcttcat gctcttgatc cccaatgatg
     2521 tctaactgat tttttatggc atccggtagt gcgggggcta ctgtctggct gtacgtaaaa
     2581 cgtagttgtt catcaattga tgcaaatccg caattttcaa taattccttt gacattgaca
     2641 ggaatatctt tcgctaatac agacatcatc gtagctgctc ccatcgaacc accataaagt
     2701 aaaatctttt gagatggccg ctctcgaact tcttgattta tccagtaaag gacgtcatct
     2761 gactcatatt gtccataggt cacataatcg cctccgctat taccagtcgc tctggaatca
     2821 acaaataata aattataccc ggccttataa aaatcattgt attcttctgt aacgatcccg
     2881 ttattccaat tccctcgaaa tccgccatgg atcatgactg ttttatctga accttgatcg
     2941 acataatatg catatacatc catcattttg ccatcttgtt tgtttagtac agtttgatga
     3001 agttgttttg tatttggata gaagcctgtg ttttctgtag gcgttgtccc atcatctgta
     3061 cctccaagca tcgacatcaa cacactgttt tttgcaaaag cgatatcgta caatagataa
     3121 gtagcaatca gatgtttgat aatctcttta tctacaccat aatcattaac agcaagctca
     3181 gtgactaata actctattaa ttttgacttg atgtcttctg ttggtccatt aagtatttct
     3241 gtcatctgtt taccgaagta attcaaatat tctttaccat tgaaattttc acctttaccg
     3301 atatcatcat tttctgtccc attctctttg tcacttactt tagcagaaga ggattcgtcg
     3361 tttgacattt ttgtagctgt tgcttcttct gtttgttggt tatctgtggt agcggtcgtt
     3421 tcttgatctg tgctagtaac agatgtctca accgttgttt cagtatttgt ttctgtttgt
     3481 gaagtactcg atttcgtttc atttccagca tcagtagttt ctgtggtttc tacctcccga
     3541 gattccgatt cggaagaaat gctctctcca tatactgtcc attgcgggac gcttgtactt
     3601 acagcaaata agatgaccaa cgctcccttt aatacttgtt ttgttctcaa gctattcact
     3661 cctttttatg tttctaggag aattatatgt aaacgcaaac attttttata caaaaaataa
     3721 atggtttatc ccataaaaat agaacaggat tattttctca ttttacgtaa taaagcagaa
     3781 tggataaacc agtatccatt ctgctttttt taaacgaaga aacaacttat tgctccaaaa
     3841 atgtcagaaa tcgtttatcc caaattttct cgtaaaaagc gattgtttcc tttgctttca
     3901 taaaaggatt atcataagtt gtcgttgctc ctctacacat cataagctgg tatcctaatc
     3961 catgagcaaa cttcacagtc gtatctatac aatactctgc ttgtgcccca cataattcga
     4021 tcgaatgaca gtccaattca gttaataaaa cactcaaatt agtcttataa aatgaattcg
     4081 catgggtttt tctcacaaaa taactgtgct caggaataga taattccgac aatagctccc
     4141 atggttttgt ttgtggcact aattgagcat cttcgtgttg aacgaaaata actggtttcc
     4201 caatcgtttc gtatgttttg attcttttat tcactaatgc tgtcactgtt tctaattcgt
     4261 gtatcggcga agaatcaaaa catacccctt tttgtaaatc gatcactact attgcgtcgg
     4321 ctgtgtttga catacttgtg ccttctttct tttttcttga tttaatagta tttcttatct
     4381 agctagttcc attatacgtg acaacatttt cttattttac acctctaaat ggcacttttc
     4441 gactatttct tcatgttatt atttcataaa tacttagcta ttggaggaat gtatgatgat
     4501 taaattgtat acaggttctg gctgctcgtc ttcaagacgc gcaaaacgtt ggcttatcgt
     4561 ccatgatata ccgtttatcg aacagaactt aattcgtcaa ccactaacca aaaatgaatt
     4621 tatccactta ctaaaattga caaacaacgg cgtgaccgaa atgctctcta ctcattctat
     4681 tagttataaa aatcttgttc acagaataga tgaactcagt ttgagtgaac tatatattga
     4741 gattcgtaca tccccttctt tattgaaaaa accgattatc tacgaccaat ctgcaaaact
     4801 tttgactgga tttagcgaag ataaaatcag gatgttcctt tctaaaacta aaagaaagca
     4861 agagctcaac gaactcttgc tttctggtca tcttgtcgat tttaaagaaa ataatctttt
     4921 tgcaatgtct taattttggc ataaacaatc tgcttttctt aattaacgaa atccaatttt
     4981 tccaatatgg atctattttt ctcattcctg atttttatga tttcagcttt cagacgcgcc
     5041 caatcatttt ttgtaggttg tccattccag taaaatatct gtgtattcgt ttgatcatat
     5101 gcctttttgt cgataggata atcgctaatg acaatatcag gtaactgatc agcggatgca
     5161 ctatatttta ttgggcgttc agtcatagat aatatccttt tctttgccca taattcctgt
     5221 gttttaccat actttgattg aatatagaat atgacaggtt gttcgtgttt tataaaaata
     5281 tccctgacta acatacagta ttgaaaaatc aaccgtctgt tcttttcata ataagtatta
     5341 agctctggaa ttctgcgagc taccccacta aaaaactgtt gcatgattgg atacatcact
     5401 ggaaatatat gctgaaaatc tctgtcttct gctgattttc caaaagcatc caccttcttt
     5461 tttgtcccaa aaacccttga atacaaatgg atcattgcca aattgacata tagataattt
     5521 ttttctgaag ttgtaaaaca aatcttgaag tttttttcaa aattttctac aatagacgat
     5581 tcaatccagc cccaactttc ttgtacttta tctggagtat ccaacaaatc ttcttccaaa
     5641 taggtgttca ttacaccaaa cataaaataa aggtaagctt gctctgttgg agtgatgtga
     5701 ttcatcgacc agaagtttgc ttcaaaaaaa ggctctagta tgtaatgcgt aaactcttct
     5761 tttgttatca atggatgcac aaatttgttc atctcttctg ggacatacgt aatcgagtgg
     5821 ccttgtttca ttcgttgcaa cgaaattctt agtaggctga tccatttctt tctatctata
     5881 ctacgcgtat atggcgcatg tttcgcaaat ttttctaata gttgacgttt attttttata
     5941 cttgataata tatcagaatg attttcgtcg aatgactgcc aatacatcaa gtgaaaaaag
     6001 tatcggatac ggtattcttc tccttctatc ggattacctt ttttcaagct gcaagataaa
     6061 tgatagtttt tcaaaatagt attaagttct cttaattttc tttgaatcgt acgcaaactt
     6121 atataatttt ttgactaaga ttttctaagg attgataagt acctttgtaa agtgaatcca
     6181 gtaaaatata accaatcgtt ccctctaaaa gtatagaata aactttactc agtaaaaact
     6241 gagaagaaaa ctcgactttt atcatcttgt tttctgaaag aaaagataat ttatattctg
     6301 gatacctttc gcaaaacgat tctgcttgtt gaatcaccga attcatctta gccagggtgt
     6361 agtttagatc taaagcgata tccttcacca catataattt ctcgtctctt tcataacaat
     6421 agttcaaaaa ttgatatact tgcctttcat tgatatcaat aaattcttca aacaaagtac
     6481 atactccttt ttatctatta tttagactta ttccgacagc tacatgaata acgtacaaat
     6541 agattcgtta ctgatttttc agagatacta ccatgcgcag tgtgcattgt caattattct
     6601 tgctcttatt ttccatttgt gttcctgtta tttcctgttt ctcaaatatg ttagtaaaca
     6661 aaaatagatc tggcaaagct acaacttcgc caggcaatac agcactctcg aatgaaatat
     6721 tcactcaaaa atctatcgtt ttattcaatt ttagatctgt tgcttttatt ctttatttca
     6781 ctcgatggaa gagagaacga aaatagcttg ctcctacaat caaaatgagt aacacaccca
     6841 tccatgtaat tagcttgttc aaggttctac taccagtttc tggtaaatcg ctttttctat
     6901 tatctgtttc tttcgctttt tcttgatgat caattccacg agaaacaaaa tcaggctgtt
     6961 ttagcacact ttttaggttg ctccctgcat ttctcttttg ttccttagtt ggtatgatag
     7021 gtaccaaatc aagttcatta tttacttttc ctgtgctgct ttcgctgctc gtgcttcctg
     7081 tgctactctc actactcatg cttcctgtgc tactttcact actcgtactc tccgtactgc
     7141 tttcgctgct cgtgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
     7201 tgctcgtgtt ttctgtgcta atttcactgc tcgtactctc tgtactgctt tcaccgctcg
     7261 tgttttctgt actgctttca ctacttgtgc tatccgtact gcttggcggt ccaacaactg
     7321 tccaactgct tggtttcaaa ttctccgtga tcgtcatctg tccatcataa aaaacaatca
     7381 aatcaacttt atctttactc ttgatctcat caatcgatag ttctgcagaa atcattattg
     7441 tatactcaat accagcggtc cattgtacgt cttttaaata aacttcccca ataacttttt
     7501 ctgaacttcc attaccatcc caaccatatg ttagaacagc atgtacatca ctagcattca
     7561 cgcttttgtt tggtttgact ggaatagtaa tgacttgttg tgtcatgtca taaacaatcc
     7621 gatcagtttt cacagtaaat gaaagattct catttgcccg ttcatttgca gaagtactga
     7681 ttccaatcat tgctgcaatt atactcagcc caagaataac aaataatacg gtcgctgttt
     7741 tcttcatttc tatcactctc catataatta ccttctattt gataaaggaa ttatataaaa
     7801 tgaaaatgat gagaacacga cagtcagttg tgaaaatcta acaaaagatg tcgtgtattc
     7861 gcttgaattt gtttactttt tcgtttaatc aatgacagac ttaaaaaagc aagaccgata
     7921 tcagccttgc tttcttcatc ttttattgat aaacagcagt gtatttcttc atccgctttg
     7981 ttttatatgc atagagtaac aaaccaacaa tcaagactaa caagaataat gtagttgata
     8041 tagatgcttc agccccaaag tttcccccag taattattgt attttgcgat tggatcagag
     8101 tggagaaaaa accttgttct tgcgttccgc tgactggtat acccaaaata gctccttgtg
     8161 tccaattcca agtaatatga gcaccaatag ccgtaaataa acttcctgta ctgtagataa
     8221 gtgcgctgaa aaagcaacct gctaaaaaga tattgaaaac tgccagaaat gtcatgttag
     8281 gattcaatcc gtgcaaaaga gcaaataatg cagcagatcc aatcattgct attaacggac
     8341 caaatctaag catctgatgc tgaaaaaagc cacggaataa aagctcttct gatgccccca
     8401 ctgcaataaa taaaataaaa ctcgttatca gcggcacaat gtttgcgttt cctgaatcta
     8461 accgatacgc ccccgtcaaa tataatggac tagtaataag cagaaacact ccaattccaa
     8521 caacgagacc tcccgctaaa tacccccagt cttttcctcg aacagaaaca ccataatctt
     8581 ctattctttt tccttcgacc ttactagcaa gaaataaagc tagtaaaaaa acagctaaat
     8641 gtccgaccgt accggcaatt gctccaatcc attcactatt tccaataaac atatccgcaa
     8701 gtgcattatt gacaagaatt cctaaaacaa aaccaacaaa gactattaaa acagatccaa
     8761 tcaaccgaat aatcctgatc atctttcatc cctcctttat attaagttta acaacccttt
     8821 tctttcttgt ctcgtgaaaa ctatgtaaat gttctccatc atagagagaa tagattttta
     8881 cgaactaaga atatctacag caaccttacg aaaatgaaag actgtctccc taattcttct
     8941 gttagactgt ttttaataag gaaaggatgg gaaatttttt gaaaaaacaa ctactaatta
     9001 tgtgcctttc agcatgtgca attttactat tctcaggttg tagcaagtcc aatgaaacga
     9061 ttgcaacgtt taaaggtgga acacttacag ttcaggattt ttatgataac ccaaaagtta
     9121 aatcaaataa tcaaacacta ttaaaaaaaa tgattgtctt caaagcattt gatgatgtat
     9181 acggaaaaga gatttcttca aaggaagtaa caaaagaata taatgaacaa atcaaaaaac
     9241 tcggtccaaa ctacaaggaa caattaaaag cagttggcca aacagaagag acatataaga
     9301 aactctttaa acagatgctg gctttccaat acggtttgaa agccaatgta aaattaacag
     9361 ataaagatct agacactgct tggaaagaat tctatccaga agttagtaca cagatcattc
     9421 tattttcgac taaagaagaa gcagataaag caaaaaaaga agcaaatgaa ggagaaaatt
     9481 tctccaagct tgttcaagct tatggaaaaa ataaaacatt aaaggaaact gatggaaaaa
     9541 tgaattttga ttcaaccaat ccagagattc ctactgaagt aaaaaaagca gcatttaaac
     9601 taaaaaacgg ggaagtttct gatatcattc ctgtaacaga tccaacgact tatcagcaaa
     9661 gctattatct agtaaaaatg gtaaaaaaac aggataaagg ttctaataaa gacaaataca
     9721 aatctgagct agaaaaaatt gctaccgaag caaaaactct tgatacggaa ttcatggata
     9781 aaacaatccg taaagtgatg aaaaaagaca atgtaactat taaagatcct tatgtaaaaa
     9841 atatattcaa ataaacaaat ttgacccaat tggggatgtg gcaaaatttg agaagtgatt
     9901 tttggaaatt ccttcttatt tttgaggcaa accacttttg ccacaacttc atcggggcct
     9961 ttttttattt gtaccaaaac aaatcactgg atttgttaca atcatgagga tataatttaa
    10021 aaaagttttt tatttctatc acatgaatca atgcgccatt aagtagattc tttcatgctt
    10081 ccctattttt tcacttgccg tatcgcttca aaaacaacta tcataaactc aaactcgact
    10141 gaaaagttat tttaaaaaag actaaaaata tcccttctgg acttgttctt cttattctgt
    10201 tatactagag aaacacgaaa gatattttta tgcgacactt ttcatttgtt tttatgaaat
    10261 ttattcaaaa ctggcgtaac ttcagagtta caaggatgta ttagaggtta gggaatacat
    10321 cgtttaaatg agaaataagt aatagttagt aatagtcacc caaactttaa attaatgaat
    10381 attgaatcgt cagatagcct cctcgagttt tcgagaaggc tatcgtttat tttatggaga
    10441 aattgattca gaagtaatca gcgacctttt ttagttcacc aatcgtttct gcttggaaag
    10501 tttgcaccca atcttcttgg aagcccctca ctgctttatc aatcgagggc atctctaata
    10561 cttgatctag tagctcttta cctaacgtta tcgctgagct ccagatcgaa tggctgtagt
    10621 gacttgttca caatttttga aactagctgc taatattttt gttggtcttt gcgtacgttc
    10681 gatctcagtc cgcaacgaat caatcacttt catagcatca atgccttgat tttgcatcct
    10741 gttataataa ggtgcaatat aatctgctcc attattaata gctaaatacc cttgtaattc
    10801 tgtgtaaata gctgtaccgg taatcaaata accttctttt ttcaactttt tgatcgcttg
    10861 aaggcctgct tgattcaccg gcaccttgat aaacgtatct tttcccagtt cttctatgat
    10921 aactcgagca tcctctatca tttcttcttc cgttcttcct attgtctgaa tatggatagg
    10981 aatctgtccg attatttttt tgatttcttt catatgcgag aagaagtttg acgctccttc
    11041 ttttttcaga attgtaggat ttgttgttac gcctgttaaa ttcaaaaact gttgacaaga
    11101 ataaatactt tttgtatttg ctgtatcaag catgatttcc atatagcttt cccccattct
    11161 gatcctcttg atcgatttta atgattgtcc cctttcttaa atgcggcatt tccttatctt
    11221 ctacatacat tgttcctgga agttccggct catgagatcc atcaaatttt atcgtgatat
    11281 gtcctaattc attcaaatta gtttggacaa catctcctac tgctgtgatt acatacattt
    11341 gtttatcaaa acagagcttc atatcacaag ttatttcttt tcttacctga ttcaattcaa
    11401 tcttataaca ataatctgtt aatgtttccg gtgcttcatc tccaaataaa atcattattt
    11461 tttctttcct gaatacttct gcttctatac caacatctgt tacaaccgtt tcaaagatcg
    11521 tcatc
//
LOCUS       DAYYGS010000084        11256 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000084 DAYYGS010000000
VERSION     DAYYGS010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.084"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(19..1248)
                     /gene="hflX"
                     /locus_tag="ACHA44_002259"
     CDS             complement(19..1248)
                     /gene="hflX"
                     /locus_tag="ACHA44_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFM8905515.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1250..2167)
                     /gene="miaA"
                     /locus_tag="ACHA44_002260"
     CDS             complement(1250..2167)
                     /gene="miaA"
                     /locus_tag="ACHA44_002260"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFM8905516.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2171..2908)
                     /locus_tag="ACHA44_002261"
     CDS             complement(2171..2908)
                     /locus_tag="ACHA44_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8905517.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2999..3526)
                     /locus_tag="ACHA44_002262"
     CDS             complement(2999..3526)
                     /locus_tag="ACHA44_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905518.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3706..4299
                     /gene="clpP"
                     /locus_tag="ACHA44_002263"
     CDS             3706..4299
                     /gene="clpP"
                     /locus_tag="ACHA44_002263"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFM8905519.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4403..4888)
                     /locus_tag="ACHA44_002264"
     CDS             complement(4403..4888)
                     /locus_tag="ACHA44_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFM8905520.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5041..5238)
                     /locus_tag="ACHA44_002265"
     CDS             complement(5041..5238)
                     /locus_tag="ACHA44_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905521.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5333..7093)
                     /gene="efrB"
                     /locus_tag="ACHA44_002266"
     CDS             complement(5333..7093)
                     /gene="efrB"
                     /locus_tag="ACHA44_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HFM8905522.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7090..8820)
                     /gene="efrA"
                     /locus_tag="ACHA44_002267"
     CDS             complement(7090..8820)
                     /gene="efrA"
                     /locus_tag="ACHA44_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HFM8905523.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9213..9425
                     /locus_tag="ACHA44_002268"
     CDS             9213..9425
                     /locus_tag="ACHA44_002268"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit epsilon"
                     /protein_id="HFM8905524.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9427..11106
                     /locus_tag="ACHA44_002269"
     CDS             9427..11106
                     /locus_tag="ACHA44_002269"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HFM8905525.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1972 g         3590 t
ORIGIN      
        1 tgttaaaaaa ataaaatttt attggttttt tcttaaccat tttgaattgg tttttgcaaa
       61 ccctttaact tcatattcgt ttgtttcttc catgtacgct tcgtccaaaa ccattgtttg
      121 tcttctcaac tcgcttaatt tctgtccttc gttgcttggc acagaaagtg tataaggggt
      181 cattagttcc atcagctgac gtttgattgc ttcgatcaac gccatcttac cttctttcgt
      241 ctgagtagaa atcaatacat ttgggaatag cgtaggcgtg aacatcgctg ggtcgatctg
      301 gtctgcctta ttgtaaacag tcagtatcgg catttctttc atattcagtt catccatcaa
      361 ttttaagaca gtctgctcct gtaaaatacg atctggagaa ctagcatcga ctacatgtag
      421 caaaagatcc atattttgac tttcttctaa tgtagaatgg aaagcatcga tcagttgcgt
      481 aggcaggtct tggatgaacc cgacggtatc cgttacagta atctcaaatc cttcagcaaa
      541 acgccatctc ttagtcaatg gatctaacgt tgcaaacaat tgatctttcg aataagtatc
      601 cgcttgtgtc aatagattca agatagttga cttcccggca ttcgtgtagc caataagtcc
      661 aatctgaaaa atctcgctat tttttctttt ttggcgattg cgctctcgat gagcttctac
      721 ttctttcaat tctttcttaa cagcaaaaat tttattccgt atatgtcggc ggtctgtttc
      781 taactttgtt tcacctggcc ctcttgttcc gatcccaccg ccaagacgag ataatgtttt
      841 cccttgccct actaaacgag gcagcaggta ttctaactga gcaagttcaa cctgcagctt
      901 cccttctttc gaacgagcac gcatcgcaaa aatatctaag ataagctgca cgcgatcaat
      961 taccggaaga ccaacagcat cggtaatcag ttggctctgt ctcggagtta gctcatgatt
     1021 aaagatgatc aaatccgctt catgcgcatc cgcttgttga actaattctt gtaatttccc
     1081 tttaccgata atcgtttgcc gatccacttg tggtctcttt tgtgtaattg tgaatacgac
     1141 ttctcctgat gcggtattcg tcagctgaac tagctcttcc attgactctg aaaagtaacg
     1201 ctggtttgct tcggtttcta cccctacgat gatcactttt tcttccattt tattccgcct
     1261 ccagccattt tttgattgtt tgctcaatct gacttttttg atttggctca cttataagat
     1321 caaaccactg tgcatccaaa cggttacgaa accacgtcaa ctgacgtttc gcgtatcttc
     1381 tcgaatgtag tttgacttgt tctgttgctt cttccaatgt ttctttccct tcaaaataag
     1441 ggaaaaattc tttatatcca ataccttgac tggcctggac ttctccaagc tcatacacct
     1501 tacgcgcttc tgctactaaa ccttctgcta gcatcaaatc gactctttga ttgattcgtt
     1561 cataaagaat actacgcttc gtatctaatc caatcatgag gtaatcatac aattgctttg
     1621 gctcttcttt aggtgccgtg atactatgtc ctgtcgtctc taaaacttcc agagctctta
     1681 tcactttgcg ttgattgttc cagtggattt tctcagcagc tgccgggtct ttttctttca
     1741 actgattcca taatgcttgg gaaccaattg tttcagctaa cgtttcatac ttttttctaa
     1801 tctcaggatt cgcttcttct gtcgctccta actgataatc atatagaagg gattggatat
     1861 agagacccgt accaccagct atgataggta ggtgccctcg gttcgcaatc tcggtaatca
     1921 attgtcgacc tgaatgctga aaatcagcgg ctgagtaacg ttgagtaggt tcgatcacat
     1981 caatcaaatg atgcggaaca ttattcatct cttcttcttt gatttttgct gtaccgatat
     2041 ccagattttt atagatttgc aatgaatcac cactgatgat ctctcctcca aatagttgtg
     2101 cgagttccac acttagcgct gttttaccga ctgctgtcgg accgacgatc actaatactt
     2161 tttccatatt ttacctactt ttcgttgatt gtttccaccg aagagctttc tccggaaaat
     2221 cagttattat tccatctatt ttgagttctt gacatttggc catttcttct tcatcgttaa
     2281 tcgtccatgc acgaattgct tttggaaaat cagctgcctg gactggatgt tcgaacaacc
     2341 aagtattttt tggatgtact ccttctatat aaggggtttc tgctgctagt tggatctttt
     2401 tatcagaagt actcatcaat aagtccagct gtatctccgg ttccagtaat gcgatttttt
     2461 ccaaagaatc caaatgaaag ctgcaatatg catgttgaaa aggccaatct ctacttttta
     2521 atgtttcggc taatttttct tcgataccgg gatattcata ctgatcagtt ttaatttcaa
     2581 tcattagcat ccctcggtaa tttttttgga ggagcagtcc taacacttct ctcaattcag
     2641 gaactttagc tgctggaaat tctttgctga atttgccccc tgcattcaat tttttgagtt
     2701 cttccaatgt tttttctcga atcagccctt taccgttagt cgtccggtcc acttcttcgt
     2761 cgtgcataac gatcaaataa ccatctttgc ttagatggac atctagttcg ataccatctg
     2821 cacctactcg tacagcttct gcaaatgcag gaagcgtatt ttctgggtgg gtgccgctgc
     2881 ttccgcgatg ggcatagatt tgtaacatct ttcttcactc cgttcagttc tattgtatca
     2941 ttaagaaaaa aagaaagcat taccacgctc tcatgtgcaa cggcaatgct tcaaaatatc
     3001 agctccaaac atcatctttc gagaagaaac caatcggctg gcgcgtcata gtcttcaact
     3061 gagctttttt ttcattgatc atttcagtca gactatccgt aaaattctct aaaatcacgc
     3121 gtccgccttt ataagcatat ccaccaatct tcattgtttc atctggagat aaaatcactt
     3181 ttcgatggga gatattctta aatagctcgt ctaaaatata atttggcaga taaaaactat
     3241 tttgcgctga aaatgattgg agatacgcaa aatcgtcaat cgtgatataa caatcatcaa
     3301 atgcatcaaa gctgacatat tcttccatca gcgttgtccg tttttgcaag atctcgttga
     3361 tctttttatt taattgattc agcgtctcga tttctttgac tttcaattcc aattcacgtc
     3421 tacgctgttc tttattttcc aagtgacgca tttgcagtat catgccgata tagacaacta
     3481 cagcacctgt cagcactccg ataaggccag taataccaac tatcaatctg ttgttcctcc
     3541 ttgttttgtt tctctgctta tcataccata aagcgggttt atgcgactaa tttgcctaag
     3601 ttttttattt gacctttttt gaccaaaaat gtatactaca ttttaggtat tagaaactaa
     3661 attatggtac actatttcta aacgaaatca aggaggaact ctattatgaa tttaattcct
     3721 acagtcattg aacaatcttc acgaggtgaa agagcttacg atatctactc acgtttattg
     3781 aaagaccgta tcatcatgtt aagcggccaa gtaacagatg atcttgctaa ttcgatcatc
     3841 gcccaattgc ttttcttaga cgctcaagat tctgaaaaag atatctatct ttatatcaat
     3901 tctcctggtg gttcagttac agcaggtatg gctatttacg acacaatgaa ctttgtcaaa
     3961 gcagatgtcc aaacaattgt tatgggtatg gctgcttcaa tgggaagctt cttgctgaca
     4021 gctggtacaa aaggtaaacg atttgcttta ccaaatgctg agatcatgat ccatcaacct
     4081 cttggcggcg ctcaagggca agcaactgaa atcgaaattg cggcacgcca tattttgcaa
     4141 acacgcgaac gcttaaacaa aattttagct gaacgcactg gacaaccgct tgaagtgatt
     4201 gaaaaagata cagatcgtga caactatatg acagctgaac aagcaaaagc atatggattg
     4261 atcgatgaag tcatggaaaa cagttcttca ttaaattaat ctgtttcaaa ggaagctttt
     4321 ttaagctctc ttatctaaac caaataaaaa aggatggtca actcaaactg accatccttt
     4381 tttattttat ggatgctact gcctatcttt gctccttgaa ttttccttgc gcatgcatcc
     4441 gacctatctc gacataatgc tggatgtttt ctttgttttt ttgtatttct tcttttgtca
     4501 aaggacggat cactttagct gggcgaccaa aagccaagac attaggagga ataacttttc
     4561 cttctgttac taatgatcca gcaccaatca aactattctc accaataacc gcatgattca
     4621 atatcgtggt ccccatcccg atcagcgccc ctttttcgat tgtacagcca tgtagcatgc
     4681 actgatgtcc taccgtcaca ttctccgcaa tatctacagg tgcatcatga tcgacatgga
     4741 tgattgtacc atcttggata ttcgttcttt gtcctatttt tatccaatta ctgtccccgc
     4801 gtaaaacagc ttgataccag atcgtcacat cttctgacaa tgtcacatca ccaatcactg
     4861 tggcatttga tgctataaac cgttccatat ttatttttct cctttcattt tttgtagaaa
     4921 ctaatcctat tgaataaagg tatcatagca tatatttatt tcataagaaa aaaaacaggt
     4981 aaaacctgct caggccagat gtctatttac agtatctggt aactgacgtt ttacctgttt
     5041 ttattgtaca tctatatttt cttctattac tttttttaaa attaattcgt acacttctat
     5101 ttgtgctgcc aagcgttcga tttgaaactc attttcaaaa aaatcagctg aatcaaaagg
     5161 cttcgtctga cagacgtgcg ttaaacgatc aacttctctt ttggcttcag taattttttc
     5221 atttaaaaac tgttccatct ccgtgtcctc acttttcttg ataactttac tttaccatta
     5281 tcaagaatag agaacaaaaa ataagtttta aactgtttta ttcttatatg acttattctg
     5341 cttcctcagc aaattggctg ttatacagtt tttcataaaa tccaccttgt gccaataaac
     5401 tctcatgcgt tcctttttca atgatctgac cttgatccat cactagaatc agatctgcat
     5461 cacggattgt cgataaacgg tgagcgatga cgaaacttgt ccgtccctcc atcacgcgat
     5521 ccattgcttt ttgaatcaac gcttctaaac gggtgtctac tgaacttgtt gcttcatcta
     5581 aaatcaagat tttaggatcg gagaccactg ctcgggcaat cgtcagcaac tgtttttgtc
     5641 ctaaagacac attgtctccc tcagaattga tggccatatc atatccatct ggcatcgttc
     5701 ggataaaatg atctacgttt gctgttttcg cagcatcaac tacttcataa tctgtcgcat
     5761 caagctttcc gaagcggata ttatctgcaa tcgttccttg ataaagccat gcatcttgca
     5821 agaccatacc aaatagcgaa cgcacatcac tgcgactcat tgatttggtg tcgattccat
     5881 caatccttat tgctcctttg tctacgtcat aaaaacgcat caatagattg atcaatgtcg
     5941 tcttgccggc accggtcgga ccgaccacag ctacggtttg accagctttc acttcaaaat
     6001 tcaaatcttg gattaatggt ttcttcggat catatgcaaa atcgacatga tcgaatgtca
     6061 catctcctaa aatcgtttct ggcagtgaag catctacttt gttttctttt tcttctggct
     6121 catctaaaat ctcgaataca cgtttcgttg ccgctgatgc actttgcaaa actgaagaca
     6181 attgtgtcac gttccccatt ggctgactga tttgccaaat atattggatg aatgcttgta
     6241 gttgaccgac aacgatcgcc ccagtaataa cataaaagct tcctagaaca gccataccaa
     6301 tataagtagc ataggcagtt aattgaacta gaggcatcat caagccagaa atgaatgctg
     6361 cacgaaaccc aaagtatttc aagctgtgat taactttttt gaatccttcc aatgtttctt
     6421 tttcgcgtcc atatactttc aatacactaa aacctgtcat attttcttgg acgtagccgt
     6481 ttagctcacc aagagaattc tgcatgcttt gaaaatattt ctgcgaagca ttgatcactc
     6541 gttttgagat ccagatcgat gttggaatca tgatcataga gatcagcgcc atcatcggct
     6601 ggatataaaa catcattgct gcagccaacg taattcccaa tatcgcattt acgataccaa
     6661 taaaagcttg ttgtaaagca ccgctgactg catctacatc attcgtcaca cgagacaaaa
     6721 tatttccttg ctggttttta tcgaaatatg aaaccggaag acgattgatt ttttcttcta
     6781 tgtctcttct caaatcatgc atggcacttt gaacgacatt tgtcatcaaa acaccagaaa
     6841 gtaattgcga aatacaatac cctattccaa caactgtgat ccaaatgaga catttgaata
     6901 tataagaaaa attgattgat tcgccattgg caatattacg gctgatttct gtagtaggaa
     6961 gtcctgtaac atatggcaaa gccacgttga aagccactgt cagaactgta aataggatca
     7021 ctaaaagaaa agtctcacga tacggtctga tatatcgagc taaacgtttt aaggaagaaa
     7081 aggttttttt catgctaatt cctcctttga taattgagaa gcggcaatat cataataaat
     7141 cgggcatttt tccaacaact cccgatgcgt ccccataccg acgacttctc cttcatttaa
     7201 tacgatgatc ttatctgcat gcatgatcgt tcccacacgt tgtgctacga tcaatacagt
     7261 agattctgtc gtttcttttt tcagacgagc acgtaacttc gcatccgttt gataatctaa
     7321 tgcagaaaag ctgtcatcaa aaatatagat ttccggacgg cgtatgatgg ctctggctat
     7381 tgccaatcgt tgtttttgtc cacctgaaaa attggaacca ccttctgata aatgttcatt
     7441 aaatccatct ggttttcgag aaatgaattc tgaagcttga gcaatctcag ctgcacgttc
     7501 catttcttct tgcgtagcat cttctttccc gtatcgcaaa ttatctgcaa tcgttccagt
     7561 aaatagcagc gctttttgcg gaataaaccc aatcttatct cgcaacttgc ttagccgata
     7621 atctctcacg tctactccat cgatcaagat ctttccttca ctcacatcat aaaagcgagg
     7681 gatcaattgg atcaacgtag attttccgct accggtactt ccaataaaag caacggtctc
     7741 tcctggagag gctgtaaaac tgacatttcg tatcactggc tcttgcgaat ggcctggata
     7801 agcaaatgtt acattttgaa attcgatata ccctttagtt tttgtatcag atataccatt
     7861 tggatcttct acaatcgaag gtttggcatc tagggcttct tggatacgtc cagctgaaac
     7921 agctgctctt ggatacatca tgaacacact agcaaataac atgaatgaga acaaggcatg
     7981 gaatatatac tcgataaacg caatcaaatc tcctacacga agctcgcctg catcgatttg
     8041 cactgccccg ctccagatga tcaataccat tacgatatta aataagaaat aaaaacccgg
     8101 ttgtgcaaca gccatcaaac ggaataagct ttttgaacta gaagcataat tctcattgac
     8161 tacagcaaaa cgtttttctt caaacttttc attgacgaat gcacgaatca cacgcaatcc
     8221 tgaaagattt tcccgcaaga tactgttgat tttatctaag tttttttgtt gctttttaga
     8281 taacggttca gacaccttag caatcgctac gactgccaat aatagtaaag gtaatgcacc
     8341 tactacatac aaactgagtg tcacacttgt acgcatgacc atgtacaaac tgacaatgaa
     8401 catcattggt gccataaaac cgatacgtaa aatattttgc aggaacaaca tgatctgata
     8461 agcatcgttt gttgtacgag tgatcaatga agatacgcct agattttcgt attcctgatg
     8521 cgaataactt tggatctttt cgaacaaatc atctcgaata tctgctacga tattagtcgt
     8581 aatcctcgaa ccaaaataac ctaacaagat attcaaaata ataccgatta cagtaataac
     8641 gatcataaat atcccttgtt gcttcacata atcaaaatca tcattgcgga ttcctacatc
     8701 gatcatacgt gctaaaatcg taggaagtcc taattcaatc aaaataaaac caaatacgca
     8761 tatgaaattg aaaaacaaga atttcttata acgcatggta taatgccaca ttagtttcac
     8821 tttctcatct ccttttaatc ttttgttaca ttttcattac taaaaatgat agcacaacga
     8881 gcctattaat agaacacttc tctcataaaa aagtcattat gattatagcg ggatgataaa
     8941 cattgatttt ttctttcaat ctagttgatt tctatactta tttgctgttt tatgctagaa
     9001 taacaattaa ttgttgtata ttttttatac agaaggtacg gttgaaaaaa tatccgaaca
     9061 gataagcaaa aacgaggctt gtctctttga ctcgaatcca gtattctagt caaagcatat
     9121 gaaaaaccag gtggcttgtc ccctcgtttt gtgttgtcta aaaaaatcaa ctagctaaat
     9181 cactgcttta cgcagataaa ggagacaaaa caatgatcta taaagtttat tatcaagaaa
     9241 caaaagtacg caaccctaaa cgggaagaaa caaaatctct ttacattgag gcaaaaagcg
     9301 atgtagacgc tcgccaacgt gtggaagaaa ataccccata caatattgaa ttcgttcaac
     9361 ttctagaagg aagtcatcta gagtacgaaa aagaaaatgc tgatttctca ttagtggagt
     9421 tttaatatga aactttccat caaaaataac gagacaggcg tctttgctat tggtggatta
     9481 ggtgaaatcg ggaaaaatac gtatggggta caattccaag atgaaatcat tttgattgat
     9541 gccgggatca aattcccgga agacgattta cttggaattg actatgtcat tcccgactac
     9601 agctacatca tacaaaactt acacaaagta aaagccttgg tgattactca cgggcacgaa
     9661 gaccacatcg gcggaatacc ctacttgctg aaacaagcaa atattcctat ctatgctggt
     9721 cctctcgcat tagcgttgat cacaaacaaa ttagatgagc atggcttgct tcgagatgca
     9781 gagctccatg agatcaatga agatacgatc atccgtttta gaaagacttc tgtcagcttt
     9841 ttcagaacga cccatagtat cccagatgca ttagggatcg tggtgaaaac accttctgga
     9901 aatatcgtag cgactggaga cttcaaattc gatttcactc ctgttggcga accagcaaac
     9961 ttgcataaaa tggctcgtat cggggaagaa ggagtcttgt gccttttatc tgatagtaca
    10021 aatgctgaga ttccaacatt tactaaatca gaaaaaacga ttgggcattc tattttgaag
    10081 atcttttcaa aaatcgaagg acgtatcatt tttgccagct ttgcttctaa catcttccgt
    10141 ttgcagcaag cagcagatgc cgctgtggca actggtcgta aaatcgctgt tttcggacga
    10201 agcatggaaa atgccatcgt caacggtcaa cgtttaggat atatcaaagt tccagacggc
    10261 acatttgtag atgcacacga aatcaatcgc ttgcctgcca atgaaacgat gatcctatgt
    10321 acaggttctc aaggagaagc gatggccgcg ttgagccgca tcgcgaatgg tactcaccgt
    10381 caaatccaaa tccagccaga cgatacagtg atcttttcaa gttcacctat tcctggtaat
    10441 acaacaagtg tcaatcattt gatcaatttg ctgtctgaag ctggtgcaga agtgattcac
    10501 ggaaaaatca ataacatcca tacttccggt cacggtggac aagaagaaca aaaattaatg
    10561 cttcgtttga tgaagccaaa atattttatt cctgtccacg gagaattccg catgttgaaa
    10621 gtacatgctt ctctagcaca agatgtaggt gttcctgcag aaaactgctt catcatggaa
    10681 aatggcgatg ttttagcgtt aactagcgat tctgcgcgtc ctgctgggca ttttgtggct
    10741 ggagatgtgt atatcgatgg tagcgggatc ggtgatattg gtaatgtcgt tttacgtgac
    10801 cgtcacctgc tttcagaaga aggtcttgta ttagctgtcg cgacagtgga tatcaagaaa
    10861 aaagaaatct tggctggtcc agatattctc tctcgtggat ttgtctacat gcgagaatca
    10921 ggagaaatga tcaatgaagc tcagcgaatc ttattccacg ctttaagaga atgtttgaat
    10981 ggacctgact gttcagaata taaattgaaa gaagcgatga ctggcgcgct tcagcctttc
    11041 ttattcgaac gtacagaaag acatccaatg attttgccaa tggtaatgac accagatgaa
    11101 gaataaataa gaggttatga aatcagcacc ttgtcctgaa tataagagaa aacaaatgaa
    11161 aaacatgaga agccattttt gtttgttctt attccttgat acaaggtggc tctttcatag
    11221 cctcttaaat ttatttactt tttcatacaa aaaaat
//
LOCUS       DAYYGS010000085        11173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000085 DAYYGS010000000
VERSION     DAYYGS010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.085"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            302..571
                     /gene="rpsN"
                     /locus_tag="ACHA44_002270"
     CDS             302..571
                     /gene="rpsN"
                     /locus_tag="ACHA44_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFM8905526.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            586..765
                     /gene="rpmF"
                     /locus_tag="ACHA44_002271"
     CDS             586..765
                     /gene="rpmF"
                     /locus_tag="ACHA44_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFM8905527.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            797..1174
                     /locus_tag="ACHA44_002272"
     CDS             797..1174
                     /locus_tag="ACHA44_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905528.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1195..1344
                     /gene="rpmG"
                     /locus_tag="ACHA44_002273"
     CDS             1195..1344
                     /gene="rpmG"
                     /locus_tag="ACHA44_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8905529.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1368..2351
                     /locus_tag="ACHA44_002274"
     CDS             1368..2351
                     /locus_tag="ACHA44_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8905530.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            2381..2503
                     /locus_tag="ACHA44_002275"
     CDS             2381..2503
                     /locus_tag="ACHA44_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HFM8905531.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            2521..4044
                     /locus_tag="ACHA44_002276"
     CDS             2521..4044
                     /locus_tag="ACHA44_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HFM8905532.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            4068..4307
                     /locus_tag="ACHA44_002277"
     CDS             4068..4307
                     /locus_tag="ACHA44_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905533.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(4500..4961)
                     /locus_tag="ACHA44_002278"
     CDS             complement(4500..4961)
                     /locus_tag="ACHA44_002278"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8905534.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(5156..5374)
                     /locus_tag="ACHA44_002279"
     CDS             complement(5156..5374)
                     /locus_tag="ACHA44_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905535.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            5492..7108
                     /locus_tag="ACHA44_002280"
     CDS             5492..7108
                     /locus_tag="ACHA44_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFM8905536.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKKIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(7349..8689)
                     /locus_tag="ACHA44_002281"
                     /pseudo
     CDS             complement(7349..8689)
                     /locus_tag="ACHA44_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9127..9273)
                     /locus_tag="ACHA44_002282"
     CDS             complement(9127..9273)
                     /locus_tag="ACHA44_002282"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905537.1"
                     /translation="MMDITYIPCNDGRLYLSTYIDLATRILRYYMVDSHMKKEIVVRP
                     LQIY"
     gene            complement(9312..9506)
                     /locus_tag="ACHA44_002283"
     CDS             complement(9312..9506)
                     /locus_tag="ACHA44_002283"
                     /inference="COORDINATES: protein motif:HMM:NF024673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8905538.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(9416..9748)
                     /locus_tag="ACHA44_002284"
     CDS             complement(9416..9748)
                     /locus_tag="ACHA44_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8905539.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            complement(10262..10453)
                     /locus_tag="ACHA44_002285"
     CDS             complement(10262..10453)
                     /locus_tag="ACHA44_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905540.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(10547..10933)
                     /locus_tag="ACHA44_002286"
     CDS             complement(10547..10933)
                     /locus_tag="ACHA44_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8905541.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
BASE COUNT         3707 a         1766 c         2159 g         3541 t
ORIGIN      
        1 agtttctttc atattgccgt tttaagtaga ggaaaagcta cttaaaacgg tatttgtgtt
       61 tgattggact aataaaagtg aattcttatt tttcagagcc taaactgcat attcttttaa
      121 atgatttgat tatctctatg taccgataaa ttcgttttca aatagttatc tattcatatg
      181 agttttattt catgttattt attgttgaca agagcaactc ttgttggtaa agtatcagtg
      241 tcaaaacgta acgattacga tttgaggtgt ctaaatcaaa ataaaaggag tataaaggta
      301 tatggcaaaa aagtcgaaaa ttgctaaagc aaagaaacaa atggcgatga ttgaaaagta
      361 tgcagataaa cgtcaggaat taaaagccgc aggagatcgc accgctctcg ctaaattgcc
      421 aagagattca aatccgaatc gtctgaggct tcgtgatcag acggatggtc gtccaagagg
      481 gtatatgcga aagttcggta tgtcccgtat taagtttaga gaacttgccc atcaagggtt
      541 gattcctgga gtaaaaaaag caagttggta aaaggaggaa atatcatggc agtaccagca
      601 agaaaaacat ctaaggcgaa gaaacggtta agaagaactc atcaaactgt agtaaaacca
      661 gaaatttcat ttgacgaagc gaatggtgat tatagacgta gccatcatgt atctttaaaa
      721 gggtactata atggaaagaa agtaattaaa ggagcttcaa aatagtttaa tatataactt
      781 ttaggaggga atcaaaatgg aaattattta cccaccttta gttgaagaag gattaaagta
      841 ttatcttgaa acgacccagc aatcgctaga taaatgcact ttttatcgtt cgatggtaga
      901 gagaggaatt attactgaaa cgggtttacc gacccaacag gcaattgaaa atggattagt
      961 caaagattac tatgaggacc aaggcttatc atttgatgat tttttaagaa tctatccaat
     1021 ttttgaagag tatgacgagg agttatttca atgtatcgat ggttattggg agatacctgt
     1081 tgatatgaaa gaaaatttag tttcacaatt ggaatctggc gagttaactt ttgaagatgc
     1141 acaacaaatt caagcatatc ttgaagatcg ataagtattg aaggaggaag atttatgcgt
     1201 caaaatatta ttttggaatg tgtcgaaact ggagaacgtc tgtatctcac cagcaaaaat
     1261 aaacggaata accctgaacg gttagaatta aaaaagtatt cgccaaaatt gcgcagacgg
     1321 gcaattttta aagaagtgaa atagctaagt ggtaaaaagg tgacaaaatg ggcataccag
     1381 ttacaatcgt ttctgggttt ttaggggctg gaaaaacaac gctaatcaat caagccttgc
     1441 aagtctcacc ctaccccagg gaagaaatta ttataattga aaatgagttt ggtgaagttg
     1501 ggattgatca caaattatta gttcatagcg aggaacgggt gttacaacta aataatggat
     1561 gtatgtgttg cagtcttcgc agtgatttat tagcaatatt gatttcttta ctggaaacaa
     1621 tagaagaaca tcaacaacca atttcacaaa tcatcattga aactacaggt atagcagatc
     1681 cccaaccaat cattcaaaca cttttaacag cgcctcaact aagaggaaga gtatatatcg
     1741 atagtttatt gactgttatt gatagtgaca gttttgaact tattgaaaga gaaccacaag
     1801 cccttaagca acttgtcatg gcagatcgca tttttgtgtc tagaaaacaa aacttgaatt
     1861 tatctgcact aaaaaaatta cagagagaaa tcaaaagtat taacccttta tccgatattc
     1921 gaatgttcac acatagtgaa acgattcaaa aaaaggattt ttataatttg aaaaaattta
     1981 atcatccctt agtttttgaa agtgattcat cagaaaatca ctataatggc aaatactatc
     2041 atcaacacca tcaagtacat gagtttaaat caattctctt aacttcagat caagacataa
     2101 aaaaagattt tttactccaa tggattgact ggctgatttt aaccaaccaa gggagcctgt
     2161 atcgttttaa aggattgatt gctttacaag agcaggattt tatagttgcc atgcaagagg
     2221 taaacgaaca ggttaatttt caatacacca ctcgacaaaa agaccaaacc actattattc
     2281 taatcggaaa aatgttggat gaagaaagga tcaaacagag ttttgataaa ttactggcgg
     2341 aaagcaaata atgataagta gtgattgggg gtaatttcta atggagaaaa cggatatatc
     2401 tagtgcatat cgacgactta aaagcccgaa tattaaaact agaaaaaggg cattaaaaat
     2461 tatcaaagaa cataaacgaa ataaaatgaa gaaattagca taattaagga aggaaaaaga
     2521 atgaagaaaa tcagtgtagg acttattggg atagcagctc taggattatt gggagcttgc
     2581 tccagcacta atgatgcaaa agtatcgaat gataaagatg gaaaattaga aattgtaaca
     2641 acattctatc caatgtatga tttcaccaaa aacatcgtcg gcgatgaagc gaacgtggat
     2701 ttaatggttc cagcgggatc agaacctcat gactatgagc cctcagctaa ggatatggca
     2761 aaagctcatg atgctgatgt ttttgtatat cataacgaaa acatggaatc atgggtacca
     2821 aaagctaaag aaagttggaa gaaagctggg cctaatgtag tagaaggaac aaaagacatg
     2881 attttattgc caggtagtga agaagaagat catgatcacg gagaagaaga tcaccatcat
     2941 gaactagatc ctcatacgtg ggtttcaccc aaaatggcaa ttaaggaagt ttcaaatatc
     3001 aaggatcagt tagttaaatt gtatccgaaa aaagcaaaag tatttgagac aaatgctgaa
     3061 aagtatttga ctaaactgaa acggttggat gctgactata ccactagtct taaagaagcg
     3121 aaacaaaaaa gttttgtgac gcaacacgca gcctttggat atttagcttt ggattatgga
     3181 ttgaaacaag ttcctattgc tggactaagt cctgaagaag agccatcttc tggtcgatta
     3241 gctgaattaa aagagtatgt taaaaaaaat aagatcaatt atatttactt tgaaaagaat
     3301 gctaatgata aaattgcaaa aactttggcc aacgaagctg ggataaaatt ggaagtactg
     3361 aatcctcttg aaagtcttac aaaagaacaa atggataatg gtgaagatta tgtttcagta
     3421 atggaagaca atctaaaagc gttagaaaaa acaactatgg ttgctggtga agaggtagtt
     3481 ccagaaaaag aagcgaagga tgaaaagaca gttgctaatg ggtactttaa agatgccgat
     3541 gtgaaggatc gcgaattgag tgattataca ggcgaatggc aatctgttta tccgttgtta
     3601 aaagatggaa ttttggatga agttttcgat tacaaagcaa aaatcaataa agacatgaca
     3661 gctgccgagt acaaagatta ttatactact ggctataaga cagatattga tactatcaac
     3721 attaaagaca acaccattga ttttgtagtc aacggtgaac atcatcaata cacatacaaa
     3781 tatgttggat acaaaatttt gaattatgaa aaaggaaacc gtggtgttcg ttttaatttt
     3841 gaaactgatg atgcaggtgc tggtcgtttt aaatatattc aatttagtga tcatggaatt
     3901 gcaccaagta aggcagagca tttccatatt ttctttggtg gagaaagtca agaaaaactc
     3961 tataatgaat tgcataattg gcctactttc tatccagctt cattgtctaa acatgaaatt
     4021 gctcaagaaa tgatggctca ttaaaattca atttttggag gaaatacatg gtaagaaaag
     4081 tattattgtc tatggattcc aagaatattg atgcttatcg gcgttatcga tttgcaaagc
     4141 aaatgagatt aaatgggagt acattagctg atattgctgc aactttacat gttagtattg
     4201 acacagttca tagagtatta aaacgggatc ctgattgttg ttttatcaag gtcaatactg
     4261 gaagaagtag acgtttagca aaacggcata atagcttggc caattaattg ataataccag
     4321 agtagatcat ttttccctta tttggatttg atctgctttt tattatgtct tgaattgtca
     4381 aatcaagcgc aacactttat caaagaagac ttcataaggt gttctccaat ctaaacactt
     4441 tctaggtctg ttattcagtt tttcaatcat ctgatttatc gttgagttat ctaccgtagc
     4501 tatctctttc gattttggca agtattctcg aatgagtcta ttcgtatttt aatttgttca
     4561 ttgttgccaa gggcatgagg atctggaaaa taaaagggca tattattcaa tgcttctgta
     4621 actgcgaaat gattggagaa ttcttttcct ctatctggag tgatcgtttt gaccgcctca
     4681 tttggaatac tgcttaaaag ttcaatcatt ttctcgctca cataagcggc tttctttctg
     4741 gggaccttct ttcctgctaa aaggaagcat gattttctgt ctgtcaaagt cactaaacaa
     4801 gtaccaccag ttttgccagc tacaatatcg gctttccaat gaccaaaatc acttctatca
     4861 tctgcggatt ttggacggcc atggatcgaa tgagtaaggc aaatctttcc acgagactcg
     4921 acatgatttt ttgtatgtct tgtttttcct cgatggtgca atgaacggat caggccttta
     4981 ttgccgtgtg agaggggttc tgtatcgaaa agatcattgc aaattgcacg ttagatcgtg
     5041 ataaaactaa ttgaaaagtc catatgttca tgcttcaaac gattcgagat ttcctctggc
     5101 gaccactgaa aatcgacaaa taaatgacga acaattgacc aaactcacgg atcatttaga
     5161 atgtgataac gtccgcattt ttttcttcga taagcataat ttttttccgc tttggaaggt
     5221 gaatagtttt tttcttgttt cttctcagtt catgtaaaat agtaaacggg ctatgctgaa
     5281 tgtcgttgcc aattatacta atagaaaatc cttcttcgtg aagcagaaag attctttctc
     5341 gttcgagtat agtaagatga cgatactggc tcataattat tttccttcta ggagtgttct
     5401 cgacaaactt atactagttg gatatcgatt atgactcatt tttatgtgtt gcacttaaag
     5461 tgtaaattca aggtatagaa agaggttccc tatgaaaaaa atacctatta caatcttaac
     5521 tggctatctt ggttcaggaa aaacgatact tctaaatcat attctgtacg gtgatcacgg
     5581 aaagaaaatt gctgtcattg taaatgacat aggggaagta aatgttgatg gggtattagt
     5641 cgacaagaaa ggttttaaaa gaactggaga aaaacttgtt tcgatgccga atggatgcat
     5701 ttgttgtaca ttacgcgaag atttgattgt tgaactagat caattagtgc aattttctga
     5761 cttgaatcaa attgttattg aagcttcagg aatcagcaaa ccaattccaa ttattcaagc
     5821 aattatatta gctaaaaacc ctttagacat tgatttgacc caacgattag agattggtgc
     5881 cattattata gtagtggatg taaaacgttt ttgggataat ttcgaaacgg gtgaaaagat
     5941 tcaggaaaga aaagctgaag gtaatgtagt tgaagaagaa caggatattc gtggcttgtt
     6001 aattgatcaa ttcgagtgtt gtaacatatt acttttaaat aatagtgact tagtgtcaac
     6061 caagacgatt tacaaaatta acggtctaat tgctaagtta cagtccaatg ccaaaataat
     6121 taggacaatt aaaggtgaag tagacttatc gaaaattcta aatacaaatc tttttaatat
     6181 agatgagact tcctattcag tgggctggtt caaagagtta gagcttggct atcaaaatca
     6241 tattcctgaa acagaagaat atggtatctc aagttttatc tatcgaaatc ggttactcct
     6301 tgacgcacaa aaatttgccc atcttacgga taaaaatttt cctggcaata ttacaagagc
     6361 aaaagggttt ctttggtttg cccaacatag tgatttttcg acgatctttg aaacatcggg
     6421 tagctataaa agtattgaat cattaaatta ttgggtagct agtttaccta aaattgagtg
     6481 tcaaatgatt atacaaaaaa atcccgaatt gaaggaagcc tggcatccgt tgtttggtga
     6541 acgcattaat aaactagttt ttattggtat gaagatggat caacaatgta ttaaggaaca
     6601 acttgagggt tgtttaactg attctcttaa tgcagataat ttccgaaaaa tttcaccatt
     6661 tccatggaaa aagtctaata tgtttagcca gatatcgtta atgaaatttt taagaaaagc
     6721 tggtgtcaag ataggcgatg tcataattat tggcattttg attattacct cttttattcc
     6781 cttatttgta ttctctatag aaaaggagaa tgaaacaact gaagatgcta tcaaatatgc
     6841 catcgttcga attgatggca aggaaatgga tcgcttcgat ttggataaga tcaatcataa
     6901 actcgtgaat tatcatcctg cgaagggaca atataatatt gttgagatta aagatggaag
     6961 aattcgagtt aaagaagata atagtcctga tcaaatcgcg gttagaactg gatggatctc
     7021 aaaacccggt caaacgagta tttgtttacc ccataagtta gttattagta ttgagaaaaa
     7081 agaaagcaca gattactaca tttactaatc tggaaataga tgaggaggtt tttaataaga
     7141 aaaagatatt attattaata atagcagggg ctttattaag tgtgttaagc gcttgtggac
     7201 aagaaaaaga agcggtgaca acaggcacaa gtatctttaa cgggacctgc aatggtttag
     7261 ttaacagtcg gttagattac ttattttggc tcttcgtcaa tgagtgttgg tgaagaaatt
     7321 caatagaata atcgagatac tcacacggct agacagctat cttgtctagc cgtgttgttg
     7381 ttctggcttt agtcagttgg gtagtattct tagttttctt aaaatatttt cctcgatata
     7441 aaaagattcg ttctcggtag ttttgaaagt ttctatagcc ataggcaatt cgttttagta
     7501 ctttgatatg gttattcaaa cattctatag ggccgttgga atagggaacg cgcaacgctc
     7561 gtttgatttc tgtttggtat ttcttgaagg tcgtgaacac tggttggtag tgctctggca
     7621 actccttgta gtcttctttt aggagcgcga cgaattcagg aacatcttgt cttctgataa
     7681 cggaaagaaa atcctggtag acttcgtagc ctcgctttaa ggaatcatca taagataata
     7741 gccgatcgac tatctctgct tctgtcagat gatctcgaaa tgacggacgc cagtagcgat
     7801 gctgaccttt caattcccac gcattctttt gaagtagttt ccagtatttc tttaagtgct
     7861 tcgaccgacg atcccctttt ttaaaggtct tcattacttg aattcggtgg ttggtaaaag
     7921 cccgaccaat gtgttggcta atatgaaagc gatcggcgac gattcgggca ttcggaaaac
     7981 actctttcac caatgtgcga tagggtgtat agaaatctga aaccactaga cgcacttgtt
     8041 cacggacttt tatagggtag cgcaagaaat agtcgcgtaa aggattgagt tgccgatttt
     8101 ccacaatatc aatcagttga tgggtcttgc cgtccatcat gacaaagctc atggctcccg
     8161 agacattttt cactgatttg aattcatcga aacacaccac ttctggtaac agctggctca
     8221 aagaaatggt tttaggccga aggctgcgaa gtattcgaat gacagtggtc ggagaaatgt
     8281 gcttcatacg tgcgatcaaa gacatagaaa gaggttcgct taatagctca agaatggctt
     8341 gtttgactct gcgagcaatg gagcagcgac gatcactaac tgaatcttct gctaaaaaag
     8401 tctttccaca gtgtttacat ttgaagcgct gtttgttaat ccgcagaatg gtcttgtagt
     8461 tactgacatc attcaataag atggatactt ttttccagcc ctatttaatg atcgtttgtt
     8521 cattttgata gtggcgatga aagcattcct ccggttgata ggtcaatgtg ccatcaaaaa
     8581 caaaacatgt ttcgtcattg attcgctctt tgcgaaaggc atcttcatga aagataagat
     8641 ttaggtctag aatatctagt aactctttgg taaactctga ataggacatc ttgaaacact
     8701 cctttgaatg gttttcgtcg actttattct aaaggatttt aaggtgtctg tttgcgttta
     8761 ttagaaaaaa gaaaaagtat tggcaacaga aggcgtcacc aacactagat attatagatc
     8821 ccttattttc ttcgcttcaa attgtcaagg agtcatcata aattttgtat ggattctttt
     8881 gtttgggtag tagtcttcta aataatcttg gattaacacc ttcatctcta ttttactttt
     8941 atatttgttg gagtcgatta attctcattt gattgatcga tgaaacgctt caattactgc
     9001 attgtcaaca ggagtcccag tttggctcac tgaatgtgtg atgtggtggt cgttcaacag
     9061 ctcttgataa ataaaagacc gatgctggct acctctatcg gaatgaatta ccgtaggcaa
     9121 ttctccttaa taaatttgta atggacggac gacaatctct tttttcatgt gtgaatctac
     9181 catatagtaa cgtagaatac gagttgctaa gtcaatatag gtggacaaat agaggcgacc
     9241 gtcattacat ggaatgtaag taatatccat cacccaaaca gcatcaattt ggttctgttc
     9301 aaattatcga ttcaacattt ttttaccatt tctttgattg ttttttctct accatgagaa
     9361 gatttgcgtc gatgaaatcc tcttgtgcat aaattcattt cacgcataag aacagctaca
     9421 acacgtttat tgatttcgat tccttcatcg taaagttttt gagtgatccc tggagatccg
     9481 tatcgatctt tgtgttcgta gaacactcgt cttcctcttt tagtcgttta ttttcctgtt
     9541 caagaatgta gaaatctgct ttatcaggta atctttttcc ttgtccagca aaagcatttt
     9601 tcccgtatcg acgatattct gaaacccatc gggaaattgt tggacgagag atattgtatt
     9661 cttcagcaat tttcttggta gttacttttt tctcaaggat atcctgtgaa atctttagtt
     9721 taaatttacg atcatatgtt tttctcaata gagttcattc cttccggtaa cgtaactgat
     9781 agtcaagaaa agtgtatcat agcccaactt agtatcaggg tttgtcaact aaactgtgga
     9841 agttgataga taagagtttt taacaaccaa aattctcttg actatttttg aaatcagaca
     9901 aattttgatt ggacacatag ttgaataaat ctaccgtcgt tacggaaggt aaatcgcatg
     9961 cttttcaatt ctagaggaga agtcctcgtt gaagtaccat agcccagttc tggattcgac
    10021 ctcctgccca cttcatacgt aaataaagta attttaggag tgagactgtg acaaaagtta
    10081 tttttaagct aaaaacgaat taaaaaacgt tgataattaa aatcattcgt tttagattct
    10141 atgaatcatt atagaacgct gtaaaatcga tactttataa ttataaagaa gatagtttca
    10201 aatttattgt tcgaaatttg aaactatctt tttacttatg tcacagactc ccttagttga
    10261 gttattcttt aatttcgaat ttcgagatag ctagatatat tgcaagcata cattcttgat
    10321 aacaatcttg atcatatttt ccagttctcc aggattgctt atgaatcaaa gagctaaatc
    10381 ttttgagaat tgcctcaaca gcttcttcat ttccagcctt tgctaaaatg aaatcttctt
    10441 ttaaagaatt cataaatcta ccttcttccc atggacttat aatctatttc tttgggaagg
    10501 aggagaacga gcaaagaagc atacaaaatt agttattatg cagatattaa accagcattt
    10561 tttgaatttt tttgtaaagt cgatgtttta aatttgtcgc accctgtctt gtaatcccca
    10621 gaacctttcc aatttcttta tcagttttat caaagataaa tttttcgata agaaaaattt
    10681 gttcaggttc tttcaacaaa ggaaggattt ttgctaattt tatatcttca acaaggagag
    10741 tcacatttaa aacattaatc ggttgtttaa attttatagt atctttagta aacaaatatg
    10801 gtgggagttg atcaagttgt tcatgttttt gaatttcttc ttgtttttta taataagtat
    10861 tagcggtgtt caaaatgacc ttttcaaaga atcgaatcaa ctgggcattt acgcgttcgt
    10921 cgttatctga catattgcac acctcctcca ttaagattta aagggaaaag aggcaaaaaa
    10981 cacgaaaaga agctaatgca attatgctgc gctgcgaata acttctataa gctcacatgc
    11041 attgatagga ctgctgatgt ttattttcaa aaatgttttt ttagttgata atggatagtt
    11101 atctttatac ttagaaaaga aaattcatat atcttcttaa atatcaaaca tgtccttatg
    11161 gctaagaaaa tgt
//
LOCUS       DAYYGS010000086        11128 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000086 DAYYGS010000000
VERSION     DAYYGS010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.086"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            90..1349
                     /gene="lysA"
                     /locus_tag="ACHA44_002287"
     CDS             90..1349
                     /gene="lysA"
                     /locus_tag="ACHA44_002287"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFM8905542.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1336..2136
                     /gene="dapF"
                     /locus_tag="ACHA44_002288"
     CDS             1336..2136
                     /gene="dapF"
                     /locus_tag="ACHA44_002288"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFM8905543.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2139..3020
                     /gene="dapA"
                     /locus_tag="ACHA44_002289"
     CDS             2139..3020
                     /gene="dapA"
                     /locus_tag="ACHA44_002289"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFM8905544.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            3017..3733
                     /gene="dapB"
                     /locus_tag="ACHA44_002290"
     CDS             3017..3733
                     /gene="dapB"
                     /locus_tag="ACHA44_002290"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFM8905545.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3739..4434
                     /gene="dapD"
                     /locus_tag="ACHA44_002291"
     CDS             3739..4434
                     /gene="dapD"
                     /locus_tag="ACHA44_002291"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HFM8905546.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4450..5451
                     /locus_tag="ACHA44_002292"
     CDS             4450..5451
                     /locus_tag="ACHA44_002292"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFM8905547.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5448..6809
                     /locus_tag="ACHA44_002293"
     CDS             5448..6809
                     /locus_tag="ACHA44_002293"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFM8905548.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6769..7920
                     /locus_tag="ACHA44_002294"
     CDS             6769..7920
                     /locus_tag="ACHA44_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HFM8905549.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7895..9064
                     /locus_tag="ACHA44_002295"
     CDS             7895..9064
                     /locus_tag="ACHA44_002295"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8905550.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9239..10618
                     /locus_tag="ACHA44_002296"
     CDS             9239..10618
                     /locus_tag="ACHA44_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8905551.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10899..11128
                     /locus_tag="ACHA44_002297"
                     /pseudo
     CDS             10899..11128
                     /locus_tag="ACHA44_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3702 a         1945 c         2494 g         2987 t
ORIGIN      
        1 tttgtttaat attattccaa taaaattcaa atctttatca aatgaaattc caataaaaac
       61 ggaaaaaaag tgaaaagagg aatgaaaaaa tgacagaatt gataattggc ggagtcacag
      121 caagcacatt ggcagaaaag tataagacac ctctgtatgt gtatgatgaa gaaaagatga
      181 gacaaacgat gctgtcattc aagcgaggct ttgtatcgaa acgttttgat acaaaagtgc
      241 tttacgcctc taaagcattc caaacgattg agatgctgca tttagttcgt gaatatggat
      301 tcgggctaga tgttgtcagt ggtggggaaa tctataccgc tttgcaagct gattttccta
      361 gagatactat ctacttccat ggtaacaaca agacgccaga ggaactgatc tatgcacttg
      421 aaaatgattt gttgcatatt gttgccgata atttgatgga agttgaatta ttagcagaat
      481 taagccaacg ctatttaaaa aaaatctgta tcatgcttcg gttgaatgtc ggagtagaag
      541 cacatactca tgaatatatc gtcactgcgc atattgattc aaaattcggc atgagctatg
      601 aaagtaaaga gtgccaacaa gcccttcagt tgataaaaga aagcagctat ctagagttag
      661 aaggatttca tgctcatatc gggtcacaaa tttttgatgt tactgcttgg ttagctgaaa
      721 tcgacaaatt gacgaattac ttaaccgatt ttgaccaacc tctttccttg aatcttggcg
      781 gagggtttgg gattcgttat acacaaaatg atcagccgat cccagtcgaa gaggcactaa
      841 aaaaactgat tgaatatacg gaagaagctt tggaaaaacg aaaactgacc attcgtcaat
      901 tactgatcga accgggtaga agtattgttg gagaagctgg tacaacacta tacacgattg
      961 gtttcatcaa aaagacacca cataaaaaat attactttgt agatggcggg atgacagata
     1021 atatccgtcc agctctgtat caggcagaat acgattgtga tttagcagca aaactgtcgg
     1081 aagaaaaaac agagaaagtg acgattgcag gaaaaatgtg tgaatcaggc gatgtggtaa
     1141 ttaaagaagc ttatttgccg aaagcagaat cgggtgatct tttagctgtt tattccacgg
     1201 gagcttatgg atactcgatg agcagcaact acaatcgagc gacacgacca gcagttgttt
     1261 ttgtcaataa tggccaatca agactgattg ttcgcagaca agcattttct gatctactaa
     1321 ggggggaagt ttcctatgat ccttcataaa tataatggct gcggaaatga ttttatctta
     1381 ttagattacg cagaagacac agattatagt caattagccg cttctttatg cacaaaagaa
     1441 aaatatgata tagacggatt gatcgccgta aaaatcgatc cgttggaaat gatctattac
     1501 aacaaagatg gcagccgcgc gccgatgtgc ggaaatggca ttcgctgttt tgctcgatac
     1561 gtaaatgatc agaacatgat gaatgaacag gaattcaacg tagagacatt agcaggcaca
     1621 agaaaaatta cggtgttgga tccctcacct ttttattgca gcgtagatat gggagtgcca
     1681 gatgattctc cggaaaaaat caaagctaat caaacttatc caataaaaaa ccaggaaata
     1741 gagatcaatg gtgaaaaagt aacgatcact agtttatttt taggaacgat tcataccgtt
     1801 gtttttgttg aaaatgcgcg tgaggaagtc tggagaaaaa gaggtgaact gatctgtcat
     1861 catcctttgt tcaaagaaaa aaccaatgtc aactttgtag aagtagtcaa tgaacaagaa
     1921 ttgatcgtcc gtacctatga aagaggagta ggttggacac ttgcttgcgg tacaggttgc
     1981 tgtgcctctt atgtggttgc taaggaaact gggaaaattt cagcagatca ggtagttgtt
     2041 cacttggaac aaggagactt gatgatctct ggtgaggatg cgatacaaat ggctggccca
     2101 gcagttcatg aatggacaag aatattggag gaatgaagat gataaaaggt tcgatcgtgg
     2161 cgctgatcac gccgatgaat gaagagggat cagtagatta tgctggtttg gaaaaattga
     2221 tccaattcca tttagatgaa caaacagatg gattattagt tttaggtaca acaggagaat
     2281 cttctacgct cacacaatct gaggaggagc agatccttca actgacagta aagaaagtgg
     2341 ctggccgtgt gccggtgatt gcaggagctg gaacgaataa tacgaaagaa acgattgaaa
     2401 aagcgaagca ttttgcttct ttgggggcag acgcactttt ggtcatcacc ccttactaca
     2461 acaaaacaag tgatgcagga ctagctgctc attttacagc aattgcagaa gcctcaccga
     2521 ttccgcttat tttgtataat gttccttcta gaacaggcat gtcgattcca atccatgtat
     2581 tagtaaattt agcagaacat cctaatatca ttggtttgaa agaagcctca ggagatatgg
     2641 cttatgtgat ggatgccgct cggttgattg gagaagaatt cttcctttac agtggaaatg
     2701 atgatttgat cctaccagta atgagtgtag gaggaagcgg tgtaataagt gtatgggcga
     2761 atatccaacc taaaatagtc catgaattag taaaagatac gcaggacgga agatggcagc
     2821 aagcaaaaga aaagcaatta aatgcacttg aactgatcca tgccttgttc agtgagacca
     2881 atcctattcc tgtcaaagca gccatgtcat tattagactt accttccgga cctctacgcc
     2941 tgcctttagt ttctctttca aaagagaaaa agaaacaatt agctcagctt ttgttaaaag
     3001 aaaggacggt tgaaaaatga aaatcggact gattggcagt ggacagatgg gaaatagagt
     3061 cgaagaagtt gcacaagaaa gaggagatac ggtccttctt ttacggacgg cacctaaaga
     3121 agcaactacg cttgctttta cggaactgcc agaactgctt attgattttt ctcatccaga
     3181 taatttagag aaaatccttt cgtatagctt atcatatcaa cttccgttag tcattggcac
     3241 gacaggttat acacaatcac agttccaaga aataaaagaa catgccaaac atgtacctgt
     3301 gatgtatagt gctaattttt cgttcggcat catggtgatg aatcaactaa tcaagcaagc
     3361 agctgcaatg ttgcaaggct ggcagatcga actgattgaa aaacatcata gtcgtaaaaa
     3421 agatgcacca tctggtactg caaatatgct gcttcaaaca atccaagaag tgcaaaagct
     3481 gcagcccgta tacaactggc aagggaaaaa aagagaagaa aaccaaattg gtgtacacag
     3541 tattcgtgca ggttctttac ctggtgaaca tgatgtattg tttgctgcta ctgatgaaat
     3601 atttacgatc aaacatgaat ctttttctaa tcgaatcttt gctgcaggcg cagtagaagc
     3661 agcggactgg ttgaaagatc agtcaccagg ttattacaaa ttagaagatt tattaatgga
     3721 caaaggggtg taaaaaacgt ggaagctcaa gaaatcatcc gctacattca aacggcatct
     3781 aaaaagacgc ctgtgaaagt ctatctgaat gtaaccgagc caatcgaatt taaagaaagt
     3841 aaagtttttg gtgaaggaag cagcttcgtt gtttttggtg actatgagac gattgctccg
     3901 gttttagaag ctgaatcaga aaaaatcatt gaaatcgaaa tcgaaactgc tgcaagatac
     3961 agcgcagttc ctttgctaga tattaaaaaa attaatgctc gtatcgaacc aggtgctatt
     4021 atcagagatc aagtgtcaat cggcaacaat gccgtgatca tgatgggagc gattatcaat
     4081 attggtgcag taatcggtga aaacacgatg attgatatgg gtgctgtctt aggtggacga
     4141 gcgacagtgg gcaaaaactg ccatattggt gcaggagctg tcttagcagg tgtgatcgaa
     4201 ccagcatcgg ccaaaccggt tatcgtagaa gacggcgtac tggtaggtgc gaatgcagtg
     4261 attgttgaag gtgtccatat cggaaaagat gcagtcgtgg cagcaggagc agtggtttta
     4321 gaagatgtgg cagcagaaac tgttgtcggt ggaatacctg cacgtgtatt aaaaataagt
     4381 gatgatcaga caaaagaaaa tacagcattg attgctgcgt tacgcgaatt atagaggaga
     4441 tggagaaaaa tgcgcgaaat agatacctgt attgtggggg caaccggatt agtcggccaa
     4501 acgatgctca aagtgttaga agaatatgat tttccagtag gacgattgaa attattagct
     4561 tcaaaacgtt cagccggtaa agtttgtgta ttcaagaaaa agaaacacac tgtacaggaa
     4621 ttgacagaaa cttcatttga aggatatgat ttagcgctat tttctgcagg tgcttctgta
     4681 tccaaagagt ttgcgccaat cgcacgaagt cagggactat ttgtcataga taattcctca
     4741 gcatggcggg aagattcgga tattccatta atcgttccag aggtcaatct ggaagactat
     4801 acattgaatc ggctgattgc aaatccaaat tgttcaacca ttcaagcggt acttccgctt
     4861 aaagctttac aagaagcctt tggtatccag cgagtcaatt attcgaccta tcaagcagtt
     4921 tctggttccg ggcaaaaagg cattgaagat cttcatcgta cgcaaaatga cgaggaacca
     4981 gttttgtatc cttataatat tagccaaacg gtgattccgg aaatcgatca gcctttggaa
     5041 aatgggtata caaaagaaga acagaaaatg ataaatgaga cccgtaagat tctgcatcag
     5101 cctgctttgc cagtatcagc aacctgcgta cgtgtgccaa tcgaaaatgg acatggagtc
     5161 tctgtggcag ttcaactgca aaaagagttt acgacggaag aaattagaga gtgtctcagc
     5221 cagtttccag ggattcaatt agaagatgat ctagcagcac ataaatatcc gacgagcatt
     5281 cttgctagag gaacagatat ggtttatgtt gggaggatca gaaaagacac ttctttagag
     5341 aacggattgt tgttctatac gactgccgat aacatacgca aaggagccgc tgcgaatgcc
     5401 gtacagattg ctgcagcttt aatgcagaaa atagcggagg tggcaagatg agtaatacgc
     5461 tgaaagtagc gaaattcggc ggaacatcga tgtctcatga agagcaattc aaaaaagtcc
     5521 gtacaattgt tctttcagat cccaatcgcc aagtgatcgt tgtttctgct ttaggcaaac
     5581 gtacgtcaga tgatgacaaa gtaacagact tactttattt gatttatgct taccttcagc
     5641 atcatgttga ttggacaccc ctttggaaaa ggatttgtga tcgtttcatt caagtaagag
     5701 atgttcttgc cctgcagtta cctattgaag atgaactagc acgcatacag aaacaactgg
     5761 aagacgaaaa gataactgaa gattatttag taagtagagg tgaatatctg actgctcaac
     5821 tgatggctga gtatcttggt taccagttta ttgacgcaaa agacctgatt ttctttgact
     5881 atgatgggaa aatcgatcag gaaaaaacag cacaggaaat cacaaaagca tttaaaagat
     5941 atccgaaaat ggttgttcca gggttttacg gggcgaaccc tgcaggaaaa gaaaaattat
     6001 tgggccgtgg gggaagcgac attaccggcg cgatactggc gaagatgttg caagcggaaa
     6061 gttatgagaa ttggacggat gtttcaggaa tcatgatggc tgatccgcga atcatcgatc
     6121 atcctaaaat catcgaggaa atctctttta gagaactgag agagatggct tatatggggg
     6181 ctaacgttct tcatgaagca gctgttttcc cagttcaaga ggctgatatc cccatccaaa
     6241 taaaaaatac caatgatcct ttcgctcaag ggacaaaaat atcgaatcac gaaatagaaa
     6301 aggaaaatgg actgacagga atagctggac gcaaggattt tctttccatc actttattca
     6361 aacgtcatat gtccgatgaa atcgggttta tctggaaagc aatgagcatt tttgctaaac
     6421 atgagatatc aatcgaacat attccttcag ggatcgacaa tatcggagtg atcgtctcag
     6481 cagaagcgat tgccgataag ttatttctca tcacgaaaga attaaaagaa acacttggcg
     6541 tggaagaaat cgtcgtgatc gaagacttag ctttgatctc tgtcgttggg ggtccgcata
     6601 aagaattgat tggattatct gggaaagtgt tatcgatatt gaacaaatta gaaattcgca
     6661 catcgatctt atcacaagga gcacaggagt tgaatctgat catcggtgta ccgaataatc
     6721 aatatgaaac agtagtgaag ggtatctatg aagggatggt gaatacggat gctgaaagaa
     6781 tatcgaatgg cactccatca aattcctgaa atcgggtttc atgaattcaa gacaaaaaaa
     6841 tatttatatg aacaagtaaa aaacagcgag gccgttatcc atgaaatcgg cgaaacagga
     6901 ctactccttt attttgataa tcagcaagaa gaaacgattg cttttcgaac agatatcgat
     6961 gcactgccag tcaaagaaga aacgaatctc tcttttgctt ctaagcatga aggttatatg
     7021 catgcatgtg gtcatgacgg acatatggcg atgcttttag ggttgactga ctttataact
     7081 aaacacattg aggatcttat ttataatgta gtactgatct atcagccatc agaagaaatt
     7141 tccggtggcg cacaaagcgt tattcagtca ggattgttgg ataaatatga agtacaagca
     7201 atatttggct ttcatctatg gcctggacta gctaagggag aagttttctc tcgaccagga
     7261 cctttgatgg cacaaagcag tgaaacagac attatcatta aaggccagtc tgcacatatt
     7321 gcttctagtg aaaaaggaat cgatagtctg gaggcagctg tacgattttt gaagaatgtt
     7381 tatgattttg accggcaatt accggcagag tggaatcatc taatgaaatt tggccaaatc
     7441 caaggtggaa cgatccgaaa tgttttagct ggagaagtta ttgtatccgg aagtatccgt
     7501 tcttacagtt cagatatgca aaattatttg aagaggggat tagaccattt agcagaacag
     7561 tttcactcta cagctactat ttcttttcgc tataatgacg gctatccagc tgtaatcaac
     7621 catagaggct tatatgcagc actgttagat tcaaacaaac tacatgaatt agatgctcca
     7681 gttcttcaag ccgaagattt tggcatatat acgcaacaat atccatgtgt atttttcttt
     7741 ttaggtgtcg gtgatacccc tgctttacat gatccgacgt tcgattttga tatggatgtt
     7801 ttagaaaatg gtgtagaatg gtacaaaacg attcttttta cagaaaatct tcttgtaaaa
     7861 atcgatagca gttcaaaaga ggagcgagta aataatgatg aatttatcca atcgatttag
     7921 tcctaggctt tctagaatag aagtatcaaa aatacgacaa ttcgatcagc aaatatcgtc
     7981 gataccagat gtgatcaaac tcaccctggg agaaccggat tttccaactc ctgaacatgt
     8041 caagcaagca ggaatagcag cgattgaaga agacttttct cactataccg gaatgagagg
     8101 attagaggaa ctgcgggaag cggcttgtat atttcagcaa cagcgttatg gattaactta
     8161 tgatccgcaa acggaagttt tgacgacagt tggtgcaacg gaagcgattg cctctgcgtt
     8221 gttatctgtt ttggaagaag gagacaaggt tcttattcca gcgcctgctt attcaggata
     8281 tcagccatta gtggaactag ctggtgctga attgatcccg atagatactt ctgacacagg
     8341 gtttgtctgt cagccggaac aatttgaacg agcatttgaa caatatggat cagctgtcaa
     8401 agcagtcatt ttaaactatc ctaacaatcc tactgggacg acattgtctg caaaacaatt
     8461 gaaagcaatc gctgaggttt taaaaaaata tccggtattt gttataagtg atgaagtata
     8521 tgctgaattg acttatagtg gaacgcatat gtccatagct acttatcttc cagaacaaac
     8581 aatcgtgata agcgggttat ccaagtcaca tgcgatgact ggttggcgag tgggctttat
     8641 ttttgctcaa aaagccctta ttgacgaact aattaaagtc catcaatatc tggtgactgc
     8701 cgctacaacg atgtcgcaaa aagctgcggc tgaagcgcta ataaatgggg tgaaagatag
     8761 cgagaatatg aaagagcaat atcaaacacg aagagactat ctggtccagc aattggcgcc
     8821 tttaggattt gaagtgaccc agccaaacgg tgcattttac ttgttttgca aactcccagc
     8881 tgccattcaa actgactctt ggcagttttg tgtagaatta gcggaacagg caaaagttgc
     8941 gtgtatccca ggaattgcat ttggcccaga aggagaaggt tatatccgga tttcttatgc
     9001 tagcagcatg gaaaaattac atgaggcctg caaacggatc aaagactttt taacagaaca
     9061 gtgattcata gattttatca aaaaaacaat cgttaatttt gcggtatatt ctgcaaggag
     9121 acgattgttt tttaaattta gttcaaatga acgcgtttct tttgattcat aactaatgta
     9181 atgttaagtt taaattgttt agaatgattt taaaagaata ttcaaggagg tttcatcaat
     9241 ggaaaaaatc aaagttgtca ttgtaggtgc atctcatgga gggcatcaat cgatcttgga
     9301 actattgaca cgatacggtg aaaatgtaga tatcacgcta tttgaagcag gagactatat
     9361 ttccttcatg tcatgtggta tggagctgta tttagaagac aaggtaacag atgtaaatga
     9421 tgtacgtaat ttcagaccag aaaactttcc gcaatccaat gttcatattt taaataatca
     9481 cgaagtaaaa acgatcgatg ccgataaaaa aacagtaacg gttactagcg catcagataa
     9541 cgaaacaaaa gaatatcctt atgacaaatt gattttgagt tctggtgtca aaccaaacaa
     9601 tctgccagta ccaggagcag atctagaaaa tgtctacttg atgcgtggat acaattgggc
     9661 aaccaaaatc aaagaaaaat taaatgatcc ggctattaaa aaaatcgctg tcgtaggtgc
     9721 gggttacatt gggatcgaag cagcagaagc aagccgaaaa gctgggaaag aagtcgtatt
     9781 gcttgatgtg atcgatagac ctttaggcac ttatttagat gctgagatga cagatatttt
     9841 agaacaacac ctaaatgaaa acggtattga agttttgaca aatgcaaaaa tcgaagcttt
     9901 tactggtaac ggaaaagttg aggcaatcaa aacaagtgaa aaagaaatcc cagcagatct
     9961 agtcattcaa gctgccggtg tgaaggcaaa tacagaatgg ttaaaaggga tcgtcgactt
    10021 agatgaacgt ggctggatcc aaacaaatga atacttgcaa acgaatcttc cagatgtgta
    10081 tgcagtagga gatgctacac ttgcttattc tatcccagca cgcaaaaaaa tgccgatcgc
    10141 tttagcgact gttgcacgta gagaagcacg ttatgtagta aaacatcttt ttgaagaagt
    10201 accaaacaaa ccgttcggcg gagtcgtagg ttcctctgca ttaagtgtct ttgactatca
    10261 ttttgcagca agcggattga atagctttac agcagcaaaa gcaggagtag atgtacgagt
    10321 gtcttattat gaagatacgt tacgaccaaa atatgtacca gaagaacaag gaaatccaaa
    10381 agtgtccatc caattattct cggatccaat gacccatcaa ttgctaggag gagcagtatt
    10441 atctacctat gatgttacag gtcaaggaaa tgtattggca ctagcgatcc aaaacaaact
    10501 gactttagaa gaactagcag aagctgactt cttcttccag ccaggctttg accgacaatg
    10561 gagcatgttg aatctagccg ctcaacaagc gttaggagaa gaaccatttg tagaataaga
    10621 aaacgaaata tttgaaaaga ggttgtgaaa aagctgcctt gcatcaagta ataagaacag
    10681 ccaagtaaaa acagcttctt atgtttttaa cttggctgga cttattaccg cagatgcaag
    10741 cttttgaaca ccgtttattt tcaaaaaagg gactgtgaca cgatttgtgt accgaccccc
    10801 aaaagttaga ccaaaaaatc taacttttgg aggtcggtat tttttatggt taaatatact
    10861 tttgaactta aattaaagat cgttcatgat tatttggatg gcaaaggcga ttggaccgta
    10921 aaaatccaga tcaagagatt gaagcggaaa tgcttaaaat tcgggagaag cacaaggatt
    10981 atggctgttt acggatgacc aatgaactaa gaaaccgcgg tttctctatc aataaaaaga
    11041 aagttcagcg cttgatcaag aagctaggaa taaaagtaac ggcatacacg cgtaaatcac
    11101 gtcgctacaa ttcttatcga ggaaaagt
//
LOCUS       DAYYGS010000087        10876 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000087 DAYYGS010000000
VERSION     DAYYGS010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.087"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(550..2163)
                     /locus_tag="ACHA44_002298"
     CDS             complement(550..2163)
                     /locus_tag="ACHA44_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8905552.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2304..3212)
                     /locus_tag="ACHA44_002299"
     CDS             complement(2304..3212)
                     /locus_tag="ACHA44_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905553.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3395..4519
                     /locus_tag="ACHA44_002300"
     CDS             3395..4519
                     /locus_tag="ACHA44_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFM8905554.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4746..5663
                     /locus_tag="ACHA44_002301"
     CDS             4746..5663
                     /locus_tag="ACHA44_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905555.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5656..6489
                     /locus_tag="ACHA44_002302"
     CDS             5656..6489
                     /locus_tag="ACHA44_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905556.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6572..7690
                     /locus_tag="ACHA44_002303"
     CDS             6572..7690
                     /locus_tag="ACHA44_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8905557.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7711..9582
                     /locus_tag="ACHA44_002304"
     CDS             7711..9582
                     /locus_tag="ACHA44_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HFM8905558.1"
                     /translation="MKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQIDYIHQGVG
                     YGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYWGEIHERDQR
                     MVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQFFKIIVGTII
                     QKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYGLIYSVLEPE
                     KPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAMLWADLLPFKL
                     GEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLWLNKIFLLLA
                     LPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPNFHTLTNEKY
                     LKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKTYGQFIVRNN
                     VRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLYETGFAIECS
                     PEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLMTWENTFIPG
                     LEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKNNKKRLKLC"
     gene            9576..10799
                     /locus_tag="ACHA44_002305"
     CDS             9576..10799
                     /locus_tag="ACHA44_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HFM8905559.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
BASE COUNT         3665 a         1718 c         2120 g         3373 t
ORIGIN      
        1 aaataaatag gaacaaaaaa attaaattat ttaatacaca cattagggat cattcattga
       61 aggattagaa gacaacgagg atggcgaaaa atatgattac aatagaaaag tcgttctaaa
      121 ccaatcagtt cctagtatca cacaacgcaa gccagtcgat ttggtgagat tagaggagtg
      181 aaagcaaact ttattatata agaaaaaata ttcgaaaggg atgctagttt aggtactagt
      241 gctgatagat taatatgatg cctcgtaata ttaggcgtag ttactctaga gacatcacta
      301 tggactatga tttgtctcag agtcacaaca tgttttttta tgaaaagaaa cagctaaaat
      361 aattaaattg cgcaatgttt aaactttctt aaactataac ctaataaatg tgaagaaata
      421 atgcacaatt tcttcacatt tatcttctat tctatataca taccaaccaa caaccctata
      481 tactttttca tttttactcc tcaagtaaaa ataactccta gaccgcggct acccaccgcg
      541 gtcgtttttt tattcttcaa tcaaaatcgc tttgataccc aaacctgcca agattcgggc
      601 agaagtataa gtattcaatc ctcttctgaa agtaatgtaa acttttttat cttttgggat
      661 ttcattgatt cgatctcgta actcattcat tggaatattt tttgttgccg agatactgcc
      721 agaaggtgct ttattaggtt cacgtatatc taaaaagaac gcttctttca aatcgttttc
      781 gggtatatca cttgctttga tcgtttctac gatattcgtc atttgattga ttgcgacata
      841 accagctata ttcaacgggt cacgggtagt agaatacggt ggagagtaag gcaactccaa
      901 atcaggcaaa tcaaagactg tcaagtttcc ggtgattgct acagaaagtt ctcccatccg
      961 tttatcgaca cctttttctc caaatgcttg tccacctaaa atacgaccgc tatctgcatc
     1021 aaaaatcagc tttagattta atcttgtagc ttctggatag aaataggcat ggtcaaaggg
     1081 agtgataaaa acagttttat aatttgttat gcctgatgct tttaattcat gttctgtcat
     1141 tcccgcataa cttgctgtat gatcaaagat ttttgcgaca cctgcaccaa tatatccgcg
     1201 atagcgcata ggcgtaccat ttaaaatatc tgccagcata tgtccttgac gattggccgc
     1261 actggaaagc atactttgga tcggctgacc agtcactaca ctagtcgttt cgataatatc
     1321 gccaatcgca tagatgtccg gcagattggt ttgtaactgg tcatcaacaa tgatctgacc
     1381 tgtatctgat aattgtatgc cggttgcttt caccacttca ttcttcggag aaacaccaac
     1441 agcaaaaatt agcatatcag cagaaaggac ggagccatct gataatacta tttctcgacc
     1501 tttatcacgg atctcagtaa ctcttgtttc taaatgaacg gctaatcctt ctttcagcaa
     1561 tttgtcataa actaaattag caatttctgg atcataaggg aaagcgactt gtggcaattg
     1621 atcgattaaa gtcacgtcta agccgcgatg cttgaaattc tcagctaatt ccagacccat
     1681 aacacctgca ccaagaatga cgacatgttg tggatttttt tcttcaagga acgatttgat
     1741 tcgatccgca tccgtcacac tacggaggac aaagccgttt tctgcttcaa aaactccggg
     1801 aatatccgga taatctggtc tagcaccaga agagatgatc aacttatcgt aatctgtttt
     1861 tatttcttca tttgtggata ggtctttaac tgttaatcgc ttagtagaag gatcgattgc
     1921 tgttacttcg tgtttagtaa agacatcgat gttgtttttt gttttcaata tttctggtgt
     1981 acgttcgatc agcgagtcac ggtcagtgat cacacctcct aaataataag gcaaagcaca
     2041 acttgcataa gaaatatttt ctccgcgttc atagataatg atttcgtgcg cttcattcaa
     2101 tcgacggaaa cgagtggcaa atgaaggacc gccagctaca ccgccaatga caacaacttt
     2161 cataaaaaca tctccttttc tacgttgatc gtactatgtt atggtaaaaa acagcaaata
     2221 attgtgccgg aaaattattg tttattttga atagacagaa aatatagaga aataatttgt
     2281 ggataaaacc taaaaatttt tttctatttt aaggatagat aatactttga aggggataac
     2341 cctgtatatt ttttaaaagc tgaagaaaaa tgatggatcg aatgaaatcc taatatatct
     2401 gcaatttctg tataggttaa ttcattttct agcacgagac gttttgcttt ttccattttt
     2461 agttctttaa aatagctaat aacacttttt ccattcactt ttctaaaatg tttttttaag
     2521 ctggctggac tgataaaaaa aatttgggct aattgttcaa gcaaaaaatt ttctgaaaga
     2581 tgttcttcta aatatcgtat gattggatca actaattcat ctgaataaag aatatgcttt
     2641 tttttaggaa tgactttctc atttgaaggt attgtcaata aagcaagttc ctgttgataa
     2701 tgccttggta aggaagacaa ttgaattttt tgcagacaat aagtaactaa taaggaaagc
     2761 aaatgaatgc tcatctgttg gcatagtatg aaaaaagaat tgatttcctg atcataggaa
     2821 atagctggct tactatgttt tagataatca attttgaact gttcattttt cagaaaagta
     2881 atgatagatg ttatttcttg atctatagaa aaagattgtc ccgtaatatg tgctaataat
     2941 tgctcatcaa aataaaactg gataatgata aaagtttgtg tttccttttg agtagaaaag
     3001 atttggactt cttgctgttt atcaatgaat aaaagggaag gattatctag ttctatagat
     3061 ttatgtgcaa attcagcata taaattagcg ctttcattta ttagtagtcg aaaataggga
     3121 gcagcagata aatgaagatg ggtatctatg gaataggtta gtgttttttt gacaagattg
     3181 gaggattggt tgtttgttaa caaaagaacc actttccttt ctctttttat ataaaaagct
     3241 ttctatttat aattaatgac tattataaca aaaatgaaaa gccaaaacga caagtaaaat
     3301 atttttttat ataaatattt gtgttactaa ttccgttaat atacataagt gaaagcgttt
     3361 tatataagaa cttgaaaaga aagaagggat agacatgaaa aaaagataca aattggtgat
     3421 agctttgcta aacattaccc tattattcag cttaagtgca tgtggaaaaa ctaacgaaga
     3481 agtaacagca aacatcgcag atccaatgat aaaaaaagcc actacattag aagtatatgt
     3541 cgcaaacggc aaactgacaa actatgttga aaatgctgca aaaatctata accgcaaaac
     3601 tggagcaaat attgacttaa aaattgtgaa tgtagcttcg ggaacagcaa cagtacaaat
     3661 gatcacccca aaattggtaa cacacgaaga tatgccggat attatctcca tggcagacac
     3721 ttcagcagca ggtgtgctgg aaaaatttca agatgctttt tatagtgctg atgaatatgg
     3781 attttatgac aaatatggcg atcagtttta tgatcaaaaa ttgaatattt tgcgtcaaca
     3841 aacgagtaat gaaacgatta ttccttgggc tagtgatttt accccagcac tttgttatta
     3901 tcaaccaaaa aaatttgctg aaataggtgt agattttgaa gaaatcagtt cttgggaaga
     3961 atatatccag gtggcaaaag cattaaaaga aaaaactggt agttttggaa ttgccttgcc
     4021 agaatctggg gaccaagaat tgtttatcaa catgctagcc cagcagaatc aatctttgct
     4081 tgatgaagaa ggaaatatcc gtctgaatac taaagaagcg aaacatgctg caaagttaat
     4141 caaagaaatg atcgactcag atattgtaca tttctacggt gcacaagatg ctgagaaagc
     4201 atttcaagaa tctgcaatgt ttgttgctgg cggctggtat gctacgaata tgtctttgaa
     4261 ttttcctgat gcatctgaca aatggcgcat ggctccactg attccttttt caaaagaaaa
     4321 tccaggaaaa ggtgcagtat caggaggatc aagtttttat gtccctaaag atgccaaaaa
     4381 tccacaagtg gcacaacaat ttcttacatt tatgctgaca gatgaagaat gcttagccaa
     4441 cgcactggag ctgggcgtgg ctacatctaa ctcattggcc tatcaaacag aagcagctga
     4501 gaaaaagttt gactactaag gaggacaaaa atactatgaa ctattgaata aaatcaatga
     4561 aaatacatca aatactgttt taccagccac ttattcagat gctacgtctt atttgactac
     4621 cgctgcatat aactattggc aaacaggtga ttttgaaaat tcatatctaa aagaagcaga
     4681 aaattttgct cataagtacg gcgtaaaatt aactggtgat taaaaaatat taggaggact
     4741 taacgatgat acaatcttcg ttaccaagca agacaataaa gaacagcaag aaaaaagcaa
     4801 cagatacgtc tcataaaaaa agtgcttatg gattggtcac accttttgtt attttcttta
     4861 ttgtgacgat tttgattccc atcattatct cgtttgcttt tagttttatg aaaattggct
     4921 actcatttga atttgttgga tttgacaatt atctagctat cttccgagat ccattgttta
     4981 ttaaatctta cacgaatgtt ttgattatca tggcaggatc aattccaata acgatgctat
     5041 tagctattta ctttgctgtt ttattgaatt cagcacagtt aaaaggaaaa ggatttttta
     5101 gaactgtcta ttatattcca accattacat caacggtggc tgtagcttcc gtattcatga
     5161 cttttttcaa tccgactggg ttattcaatg gatttttgaa tgcgctagga attaacggtg
     5221 tacagtggtt aacaaatcct ttttggatac gaatatcaat gatcatcacg atgatttggc
     5281 ttagtgtagg atataacact attttatttt tagctggtct tcaagggatt tctaaagaga
     5341 tatacgaaag tgctgaaata gatggagcat cgaaatttcg gcaattcttt ttattaacga
     5401 tccccatgct aaagccaatc attttaatgg ccacagtttt agccactatc aatggcttgg
     5461 gtaatttcaa tatcccaaat atattctttg ggacttccaa tggaccagaa aattcagctt
     5521 tggtcgtagg agtcaattta tataaaacaa gttttgaaat ggttgatttt ggaaaagcct
     5581 ctgctattgc ctggacgatg gtgtttgtat ctgttatatt atcggctatt caatttaaat
     5641 tcggaggtga taaaaatgac taaacaaaag atacaaagca gaatttttac gtacgtcatt
     5701 attgtgtttg gtgccttaat atttttgatg ccatatttat atatgattgt agcttcaacg
     5761 caaagtaatc aagaaatttt aagtggcgga atcaatttca agtttgggga tatgctatta
     5821 aaaaactggc aagccatcat ggcgagatat aattatccct tgattatttg gaattcgatt
     5881 gttattgctg taatcagtac agttttagct acagccagtt ctacaatggc gggttatgca
     5941 ttagcaaagt atcgttttat ggggaataaa ctgttgttca atacagttat gctatctagg
     6001 atggtgcctt tttttaccac attgattcct ttattttaca ttatgagtcg tttaggatta
     6061 tcgaatactt atgcaggtgt gatcatccct tcgattgcca gtacaacttc agtgtttatg
     6121 atgcgacaat atagcagtca atttcctaca gcactgatgg aggctgccag aatcgatggt
     6181 gccaatgagt gggtgatttt tacgaaaatc gctgtaccga ttttatcacc tacgattatt
     6241 actagtggtt tacttatctt tatgggaagt tggaatcaat atctatttcc attagtcatg
     6301 ctttcagata aagataaata tacggttccc ttaattatca aaaatatgac ggtttctagt
     6361 acaggtgagc caattaatta tggcgcaatt atgctaattt tagcaacttc agtgatccca
     6421 atggtcttga tttatgtgtg ggcgcagtct aaatttaagg agaatgacat tggagctgca
     6481 aataagtaaa aaaaataacg aatgttaacc atctagttcg ttctaggagc aactccacat
     6541 tcataaaaat taaaagaatg gcgggatatc catgaatgaa cagacgaagg aatggtcaag
     6601 aaaaattttg aatgtagctt ctgaaaagat agatgcggaa ttagacagaa tggggaataa
     6661 aattccatat atttcttaca acggaaaata tacagacgta ataaaggaca gaggaatcga
     6721 ctggtggacc aatggttttt ggagtggtat tttatggcag ctctttcaca agacaaaacg
     6781 agaaaagttc aaagaggcag caatacaaca agaacaatta ttagaatctg ccctgtacca
     6841 tttttcagac gttcatcatg atgtaggttt tttattctta ccatcggcta ttgcacatta
     6901 tcaacagtct caaaacaaac atagtcgaca actagggtta catgcagcta cggtacttgc
     6961 tggacgctat aatatttcag ggaactattt agtagcatgg aatgaccata aacctggttg
     7021 gatgattatt gatagtatga tgaatttacc attactgtat tgggcttcga gggaaactgg
     7081 tgatccgagg ttctctaaaa ttgctcggca gcatgcagat acaacagcta agtatctagt
     7141 gaggaaagat ggatcaaccg ggcatatcgc agaatttgat gcagaatcag ggaaatatat
     7201 tcggttactt ggcgggcaag gatacaatga gaattcttgt tggagcagag gaaatgcatg
     7261 ggctttatat ggattcgcgt taagttatcg ttacacaaaa aatccgtact atctagctat
     7321 agcagaaaag atagcagtaa attttattca gaaaatcgaa aaaactggtt atattcctct
     7381 cattgatttt gctgtaaaag attcgaatat tgataccagt gctggagtat gcgctgcatg
     7441 cggtcttctg gaattagcag gatatgtttc ctctgtacga tcaaaaatgt atcatgaagc
     7501 tgctatcaat atactccaaa agacagaaaa gcactatgca aattggaata tggatgaaga
     7561 tgggatcatt ggaggcggag cagaagcata ccatagagaa ctgacttata atgtgccact
     7621 gatctattct gattattttt ttattgaagg cttacttcga ttagaacagt cggaattagc
     7681 tatttggtag taaaaagagg agaaaataca atgaaacatc cagtaccact attaaggacc
     7741 agacaggatt actttgactt atttaaaaag ataaatcaac cactccagcc gttttatcga
     7801 gcaagtggcg cacaaattga ttatatacat caaggggtag gatatggaaa taaaattgca
     7861 ggaatggaag gattctctcg ggttttatgg ggagcggggc ccgcaattga ccaattagat
     7921 gaacgatggt tgaaattaat tctatcagga ataaaagctg gaacagatcc ttcccatccc
     7981 gattattggg gagaaataca tgaaagagac cagcgtatgg tagaaatgcc cgcaatgtta
     8041 ttagcgttat atcatcatga acaattatta tggaaaaaat tagatacagg agaaaaaagc
     8101 cagattattc attggttctc gcaaattttt gaatatgaat gtgcagatgg taattggcaa
     8161 ttttttaaga taattgtcgg aacaattatc caaaaatttg gttatccagt taaagaatca
     8221 gcactgaaag aagcatttga taatatagaa aattgttatc ttcaaaatgg ctggtatcga
     8281 gacagttcaa gaggtcgcca agattattat aatccttttg catttcatta ttatggtttg
     8341 atttatagcg tactagaacc agaaaagccc attagccaga tatataagaa ccgagctatt
     8401 gatttttcta gagactatat tcattttttt gcggaagatg gtgcaaatgt gccttttggt
     8461 cgaagcatga tttatcgttt tgcagtttca agcttttggt cagctatgtt gtgggccgat
     8521 cttttgcctt ttaaattagg tgaaatcaag gggttagtca atcggaatat aagatggtgg
     8581 atgcaaaaag aaatatttga tgaaaaaggt atcctgaata ttggttatac ctaccctcaa
     8641 ttgacattga ctgaacccta taatagtacg ttatctccat tatggctgaa taagattttt
     8701 ttattgttgg cgctacctga agaccatgag tattggacgg ctaaagaaat gcctatgcct
     8761 attcgtgaag gaactaaact tctttcagaa gccaatttgc tcatacaaca tgataatgga
     8821 catacatttt ttttgaatgc gggacaatta gggccaaatt tccatacatt gaccaatgaa
     8881 aaatatttaa aatttgctta ttctagtcaa tttggttttt cgataccgcg agcgaatcaa
     8941 ttgaaagcag aaatggcaat ggattcaatg ctagggattc agcgagcgga cacatatatc
     9001 acgaccagtt ttaagaatga gagcatcaaa acctatggac agttcatcgt tcgaaataat
     9061 gtacgagaca ttgtatgtaa gaaagaggta cttgcctcta catgggaacc tactttacag
     9121 gcaaaaatca gaacttggct tattcctttt gaaggctggc agattcgcgt acacaaagta
     9181 gaattagata cagaatatgt tctatatgag acagggtttg ctattgaatg ttctcctgag
     9241 aaagaaggaa taatcataga agaagacaag gaaaactata gggtgagtga agctggcttt
     9301 tcaggtatta tctgtttaag tgacgatact attaaacgaa aatccacagc tattatgggt
     9361 ttgcccaata caaacttgat gacatgggaa aacactttta ttcctggatt agagggtaca
     9421 tttggaaaag gaacccattg gctcggaaca ggagtggttg ctcatcaaga tcgggattat
     9481 agtttagaag tatggaacaa tcgtcccaaa attgattttg atgggacaac cgggctaact
     9541 atccaaaata aaaacaataa aaaaagacta aaattatgct aaaggctggt ggggaaatgc
     9601 ttgataaagt gatgtttgaa gagataatac atccagaaaa atggataaat gaatgttata
     9661 aagaattaat ggaaaaagaa tggaataatg agatcgagcg ttgtcgcaga cacacggaga
     9721 ggttaatcaa acgtatcagt aacagtcagt atcaattccc aagcgagtgt cctataaaag
     9781 gacgttatcc attaagagac aatattggtt ggacagaagg attttggaca ggaattaatt
     9841 ggttgttatt tgagaagacg ggtgatcctt tatttcaaca aacagctgaa aaagcagata
     9901 tagattttat ccacaggatt gactgtcaaa ttatcactaa tcatcacgat cttggttttt
     9961 tgtattcttt atcaaccgta gcagattata aaataacggg aaatcgaaaa atgaaggaag
    10021 cttcaattga tgctgcagaa acattacttc agcgtttttg ggagaaggca ggagttatac
    10081 aagcttgggg agatcctagt gatccttcac aacaaggtag aatgatcatg gattgttgca
    10141 tgaatcttcc attattgtat tgggcgagtg aagaaactgg aaatcaaaaa tataaaaaag
    10201 ctgctcaggc tcatattcaa aatgcagcag attatttagt aagagaagat ggttcaactt
    10261 atcatacatt ttatatggat gtcaaagatg gaaaaccaag atatggaagt acgcatcaag
    10321 gttatagtga taattcctgt tgggctagag gccaagcatg ggggatttac ggttttgcat
    10381 taagttatcg ttatttgaaa gaacaaaaat attttgattt aagcaaaata ttagccaatt
    10441 attttatgaa tcggttgcca gaagatacca tttgttattg ggatttgatc tttgatgatt
    10501 ctagcagaga agaaaaagat acatctagta tggtgatcat ggtttgtgga ttattagaat
    10561 taatagaatc aggtctatta gacaaaacag aaaagaactt ataccagtct gctgtttgga
    10621 aaattatgcg acaattgaaa gaaagatatg ctacaacgga agaagaagat ggctttttaa
    10681 cacagtctgt ttataataag cccaaaaata atggggtaaa tacaccttgt atttggggtg
    10741 attactatta tttagaagct ttagtgagaa tgactagtaa ttggtcttct tattggtgaa
    10801 aaaggtaaat atggaatttt tgtttgataa aataaaaaaa gaaaaaccaa aacagcatga
    10861 atggtttctg ttttgg
//
LOCUS       DAYYGS010000088        10751 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000088 DAYYGS010000000
VERSION     DAYYGS010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.088"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            166..981
                     /gene="thiD"
                     /locus_tag="ACHA44_002306"
     CDS             166..981
                     /gene="thiD"
                     /locus_tag="ACHA44_002306"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFM8905560.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1310..2761)
                     /gene="cls"
                     /locus_tag="ACHA44_002307"
     CDS             complement(1310..2761)
                     /gene="cls"
                     /locus_tag="ACHA44_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFM8905561.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3102..5768
                     /locus_tag="ACHA44_002308"
     CDS             3102..5768
                     /locus_tag="ACHA44_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8905562.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6132..6884
                     /locus_tag="ACHA44_002309"
     CDS             6132..6884
                     /locus_tag="ACHA44_002309"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFM8905563.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6906..8060)
                     /locus_tag="ACHA44_002310"
     CDS             complement(6906..8060)
                     /locus_tag="ACHA44_002310"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HFM8905564.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8245..10686
                     /locus_tag="ACHA44_002311"
     CDS             8245..10686
                     /locus_tag="ACHA44_002311"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HFM8905565.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         3505 a         1915 c         2306 g         3025 t
ORIGIN      
        1 taaacgacca ctattttctg atttattgca aatgtttttt caaataaatt tgattaccaa
       61 gaaaaggtag tggtatattt actgagggat actttgaaaa acatatgata gaaacagtaa
      121 cagtattatg aaaaatcttt ttataaaaat ggaggaatcg atttgatgga agatcagtat
      181 gtacaagcaa tgacgatcgc aggatcagat agcgatggaa gcgcagggat gcaagcagat
      241 atgcatacat tctttacaag aaatgtttac ggtgtttctg tcatgaccgc atgtgttgca
      301 ggtaattctt acggaatcgg agcaagtgtg acattgccaa cagattttat cgacaaagaa
      361 tttgaactga ttgcgaagga ctttcaaata cgtgcagcta aaacaggtat gctagcagat
      421 tcaaagttga ttgagacagt tgtaaaaaat taccaaaaat atgattttgg accattagtc
      481 gtggatcccg taatcgtaac gaaacatgga aatcttttac tagaagaatc tgctttgcaa
      541 agcttgaaag aaaagcttgt ccctttggcc gaagtactga cacctaattt ttatgaagca
      601 gaaaagctga ctggacggac attagcagct gaagaagatt ttgtgcaagc agcaaaagaa
      661 ctgcaagata tgggtgcaaa aaacgttatg atcaaaggac atcatgctaa aaatgcgcag
      721 caagaaacag tcaatgacta cgtattgttc gaatcaggaa aatcattttg gttaaatgca
      781 ccattttatc ctaccgaccg gatcaacgga actggcgatt cgctatctgc atgtattgca
      841 gctgagttag cgaaaggaaa aacagttgaa caagcgatcc gattggcaaa agactatgta
      901 aaccgtgcaa ttcgtgaaga aataaatgta gggcacaaat atggccccat caatcattgg
      961 gcagctggtg aattagagta aaagaaagaa gttgtgaaaa agttgccctg catcaagtaa
     1021 taagaacagc caagtaaaaa cagcttctta tgtttttaac ttggttggac ttattaccgc
     1081 agatgcaagc ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ccagctccga
     1141 gtcacaagga acaagttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga
     1201 ccgaggagtt ggcaggagaa caccgtttgt tcggaagaag ggactgtgac aagatttttg
     1261 tcacagtccc tttaatctta gtatattttt ctctacttga ataaaaaaat tataggattg
     1321 gagaaaataa tcgagaaatt tgttgcttga ataatagcca gttagacatt tccttgactg
     1381 tttcagttgt catggatgaa caatgtgtct catcttcttt gaactgagta gcaagttctt
     1441 ctaaaaactc aggatcgtaa atgacagcac ttgtttcaaa attcaatcgg tagctacgaa
     1501 tatcttgatt tgctgaacct accatgcaga tttcatcatc cattatcaat gtttttgcat
     1561 ggaggaagcc gttctcatag acaaggattt gtacattttc cttgatcagc tgccgagcat
     1621 aatattgtgt tgctcgataa ataaaaggat gatccggttt gttgggaatc atgattttca
     1681 catctacacc agaggcagtt gcgattttta aagcagcaat gacactttca tcaggaacaa
     1741 ggtaaggtgt ctgtatccaa acacgttttt tagcagaagt aatcaatttg ataaatgaaa
     1801 gcttgatttg ttccctttcg ttattcggtc cgcttgaaac aagctggatg gatgtatcag
     1861 caagcttaga ttcatctcga tctgcttttt taaagaaata attcaaatga tagccgacct
     1921 ttttttcttc aggtaccgag acgttccagt ctgttaagaa gcgaagctgg agcaaagaag
     1981 aagcggcacc gaaaatccgt atatgcgtat cgcgccaata gccgaatttt tttgttgtat
     2041 ttacatattg attggcaata ttgaaaccac cggtataact aatctttcca tcgatcacaa
     2101 cgatttttcg gtgatcatgg taattcaatc ggaaacgaag taatgccctt tgtgaggtaa
     2161 caaatgtatg gacatgtcca ccatttttga ttagacgatt gaaatctttt gcttttgtgc
     2221 catgagagcc aaatgcatca tataatattc gaacttccac gccttctgcg gctttttctt
     2281 ctaaaacatg taagacttgc tggccgatat tatctgtcac aaatgcgtaa tattcgatat
     2341 ggatcgaatg tttggctttt tttagatctt gaagcagtga atccaatttc tcttgtccgt
     2401 ctgtgagaag agtgacagaa ttcattcttg tcagcggcat acgatttaat gaagagaaga
     2461 agtcaacaaa ttgctgtttg tctttatcgc ccatgtcagg atcgtaatgt tcaatggtat
     2521 ctcctctaaa agactgaaag ttttctaatt ctttcaagtc actttgtcgg agatagaatt
     2581 ttttcttatc cgttaatcca cgtccgaaaa ataaatataa tacaaagccc accccaggaa
     2641 gggcaaacaa tactaagagc catgcccaaa tggctgctac atctcggggt ttgatcaaaa
     2701 tagccaccaa agcaataagt gcatttaata gataaagggc ggttataata ctagatacca
     2761 caaaaatgtt cctccttaca tacaataaca ttagtatagc atgatagaag aaagaagcct
     2821 accaaggtga atatactttt cactcctttc atgaaaatgt aggtaaatcg ggctaaagta
     2881 gttgcttttt gtaggttaat aaggtaggat attagtacag ttgaatatct ccttagggga
     2941 gtaaccggca acgtttgttg cgataatatc aacagtaagt taaccaaatg gttttctggt
     3001 attgtatgaa atggtgagac ctaagacaaa tgcgtatttg tcttaggtct cttttttttc
     3061 agacaggact agggccaaga tcaaaaaatg ggggatgaaa tttggaaaac aaggaacaag
     3121 cacaacaagc ggtctataaa gaaacgatcg actcgttgat gcagcaaacg cagagctcaa
     3181 ccgatggatt gagcaccacg caagcgaagg aaaaattaga aagagatggc ttgaaccagc
     3241 taaaagaaaa gccgaagaag ccaacttgga aattgttctt agaaactttc aaagatgcaa
     3301 tggttatcgt attgctgatt gtcgcactga ttcagatggt catgggtgac tacatcgaat
     3361 tcttagttat ttttgcagta ttgatgttga attctataat cagtgttgtc caaacaaaaa
     3421 aagcagaagg ttctttagaa gcgttgaaaa acctttcagc gcccgatgct cgagtgattc
     3481 gtgatggtaa ggaaaaaacg attccggcaa acgaacttgt ggtaggagat atcgtgttgt
     3541 tagacgcagg cgattatgta cctgctgatg gacgtttact agatgcggga tcattaaaag
     3601 tagatgaagg tatgctgaca ggagagtcga cttctgcaga aaaagaagta acggatatca
     3661 caagtacggt accaatcgga gatcgtacaa atatggtatt cagtggaacg atcgtaactt
     3721 acggacgcgg taaattctta gtgacttcaa ctgggaataa tacggagatc ggacaagttg
     3781 ctggcctttt ggaatcaaca actgagcaag tcactccgtt acaacgaggt ctggataaat
     3841 tcagtaaaaa actaagtttg gctattttgg cattgtcttt attgatttta ggtatccaac
     3901 tgtttcgtat ttatcttgga gaaggtacag gagatatgac tgcagatatc gtcagcgcag
     3961 tgatgtttgc cgtagccgta gccgtagctg ctattccaga agcattacag tctatcgtaa
     4021 caatcgtgtt atctttggga acgaataaaa tggcaaaacg tcatgccatt attcggaagc
     4081 ttccagcagt tgaaacactt ggttcgacaa gtatcatctg tacagataag actggtacat
     4141 tgacacaaaa caaaatgaca gttgttgact actttttagg taatggcgat actggggatt
     4201 tcacaaatga cccttcaaaa tggacagctg acgaacgtcg cttgatcgaa atcagtgttt
     4261 tagcgaatga tgcatcgatc agtgaagacg gtactaaatt gggcgaccct acggaagtag
     4321 cgtttatcga ctttagtgaa aaattaaacc agccttatca ggaaatccga aacaactacc
     4381 caagacaagc agaacttcct tttgactcag atcgaaaatt aatgtctact ggacacacaa
     4441 ttgataacaa aaattacttg tttgttaaag gcggaccaga tatcgtcttc caacgcagta
     4501 aaaaagtatt gctaaatggt gaagtcgttc cgatgacgga tgaattgatg aaaaaattcc
     4561 aagagcaaaa tgaagctttt tcaacacgcg cattacgtgt actagctttt gcttaccgtc
     4621 cacttgataa ttttgattta tcatttgaag atgaagacga tttgatcctt gttggtttgc
     4681 ttgctatgat cgatcctcca cgtgctgaag tgactcaggc ggtaacagaa gcaagaagtg
     4741 caggaatcaa aacaatcatg attactggtg accacaaaac aacagctgtt gctatcgcaa
     4801 aacaaatcgg gatttttgaa gaaggaaacc tggctttgac cgggacggaa ctagatgaat
     4861 tgacagaaga tcaactaaac gaagttttag aaaaagtcac tgtttatgca cgtgtttcac
     4921 ctgagaataa aattcgtatc gttcgtgctt ggcaaaataa aggtcatatc tcagctatga
     4981 caggtgatgg tgtaaatgac gcaccagcat tgaaacaagc agacatcgga attgctatgg
     5041 gaacgggtac agatgtggca aaagatgcag cagcgatggt attgacggat gataactttg
     5101 cttctattgt cagcgcagta gaagtaggac gtaatgtgtt tgataatatc aagaaagcta
     5161 tttcttatct atttgcagga aatttaggtg cgatcattgc gatcatcttt gccttagttg
     5221 ctgactggtc caatccattt acagcattac aactgttgtt catcaattta gtcaatgatt
     5281 cactgccagc aattgcatta ggtacagaaa aagctgaacc tgctacgatg aaccgcccgc
     5341 caagagatcc aaattcagga atctttacgg acaaaacatt gatttctgta acgtatcgtg
     5401 gtgtgctgat tgggattgct gttatctttg ctcaatggct tggcatgcag cattcacctg
     5461 aattcggtgt agccatgtca ttttcaacat tgatttggtg tcggacattg caaacattcc
     5521 cagcacgttc aaacacacaa acagctatcc aagcagggtt cttctctaac agaagtgtct
     5581 tactagctgt agttgtatgt agcgcgcttt actgcttaac aatgatccca ggactgaaag
     5641 aagtcttctc tatcccatca gcatttggat tgacagaagt ctttacatgt atgggaatcg
     5701 cacttgtgac aattgttttg atggaaatca ctaaattgat cattgtacga gtacaaggaa
     5761 ataaataaaa cgaatcaacg aggttgtgag cctgccgttt aattctgagt cacaaggtgc
     5821 aatttcaaca aacagcttct catgtttgtt gaatattgtg acttgtgacc gaagagttgg
     5881 caggagaaca ccgtttaagg aagaagttgt aaaatcagca ttttgagcta atactgaggg
     5941 tcatctgatt ggacactgtt tattcggaaa gagggacttt gacacgactt tggtcacagt
     6001 cccttttttt atttatgata gacggaaagc caatcatttt attattcatc aaaagagata
     6061 gaatagataa aagaggagaa tgatttattc cgaaaagtga aaacatataa caaaaaatta
     6121 gaggaaatca aatgaatgaa aaagaatggg atgaatttgc tgaagaatat tatgaaatcc
     6181 agcaagaatc gcaagtaact attgctgaag atgtaaaaaa ttatctgaaa acggagaaag
     6241 tcctgcctgc tgaaacagca gcagacgttg ctggaggatc tggcagattt ctttccttag
     6301 caaaagaaat taatcgctat gatttgatcg atttttcttc aggaatgcta gaaaatgcaa
     6361 aaaaagaagc agaaaaaaat gagttgccaa atgtttcctt tatcaaacag tcgttagaag
     6421 aatttatcca atcaaatgag acatatgaaa tggtttttac agcagcaaat ccagcattgg
     6481 tacatcttat tcaattagaa aaattgatga aaaagtcaag caaatggtgc gtgataatca
     6541 gagtagtgga atcaatcgat gatgtttttt atcctatcga aaagcaacta ggaatgatag
     6601 aagaagatcc taatacatcg ccagttgtaa tggatcaatt tgaaacatac ttaattgatg
     6661 aaggattcaa agtgaagaaa aaagatttta cttattttac acaggaatca attactgaag
     6721 aattgatgaa gctatactat gacgcgtacg caaaagatcc tgttgtgacc aggtacattc
     6781 gcaaactatt cagtgaaaaa cgagaaatcc ttagtacgac taagctgacc tatagactgc
     6841 tcctttggtc cgcagataaa aaaagaacct ttcacgactc gtgaagggtt ctttttatga
     6901 ttttattata ttttgtagta acgaatatca ttttcgatct ttgtaatcga aaaaggtaca
     6961 tctgaagtat attctgcaca ttctccgtgt tcaggaagcg tctcactgaa aatcgtttca
     7021 tattcgtcaa cagtgatccg tgttcgagaa tctagcatct cttgatgaga ttgtgcgaag
     7081 agatattctt gatagttttc ttccagataa cctgtaaaaa attcacttac tgcaccagaa
     7141 ccgtagctga atagaccgat gcgatctcct ggctgtagtg atttcgaatt ttccagtaga
     7201 gaagttagcc ccaggtataa tgaaccagtg taaagattac cgattcgtcg gctgtaacgg
     7261 atgctttctt catagcgagc cataagacgt tcctgatttt cttcgtctgt ttggtctaat
     7321 acgctttgca atgccttttt tcccatctta gtatacggaa tatggaaagc aatcgcctga
     7381 taatcttcga gtcctcgacc cgacaattct ttatgtcgat tccaaacttt ttggaatgat
     7441 tcgatatacg tagaattaga taaaggacca tcaacaacag gaaattcgct ataatctgga
     7501 cgccagaaat catagatatc ttctgtcaga aatacgctgt cgtcttcaat cgataaaata
     7561 cgcgggtttt gagtgatcat catcgcaaca gcaccgacac cttgcgtcac ttcaccaccg
     7621 cttgccaagc tgtaacgagc aatatcactt gctatgacta gtacttttcg ttctggatga
     7681 tttttgacat attctttcgc catgtgcaaa gctgcggtgc caccgtagca agcctctttt
     7741 acttcaaaag aacgagcaaa agattggatt ttcagtaaat ggtggataat taccgcactc
     7801 gcttttgaat gatcaatccc ggattctgtt ccgacgatga ccatgtcgat tagctcacgg
     7861 tctttttctg ttacgatttt gctggcagca tttgctccta gtgtaatgat gtcttcattt
     7921 gcacgattga ctgccatttg gtcttgacca atccctatat ggtattttgc aggatcatcc
     7981 ccacggcttt ctgccaactc tgtcatatct aaatataaat taggaataaa aaaggaaaga
     8041 cgatcaatcc ctattttcat ttcaacacct ctttacttat gataaaaaac ttactttact
     8101 aaggtatcat aaaaagatta aaagaaatat aaaaaagaga atatttaagg attgttaaaa
     8161 tttaatctta gaaaaaaata actggaacaa gagctatttg tttttacagc aaagaatggt
     8221 atatttttgt aggaggtggc ttacttgaaa gaagtcgtta tgatagatgc tgcaagaacg
     8281 ccgataggaa agtatcgcgg aagtttaagt ccatttactg cagttgaatt aggcacactt
     8341 gtgactaaag gattattaga taaaacaaaa ctgaaaaaag ataaaatcga tcaagtaata
     8401 tttggaaatg tcctgcaagc tggtaatggt caaaatgttg ccagacagat tgctttgaac
     8461 agcggtctcc ctgtagatgt cccagcaatg acgatcaatg aagtttgcgg atctggtatg
     8521 aaagcagtga tcttagcacg ccagctgatc caattaggtg aagcagaact tgtgatagcc
     8581 ggcggaacag agagtatgtc tcaagcacct atgctgaaac agtatcagtc agaaacaaat
     8641 gaatatggtg aaccaatttc cagtatggtc aacgacggat tgactgacgc attttcaaat
     8701 gcacatatgg gattaaccgc agagaatgtt gcaacacaat tttctgtgag cagagaagag
     8761 caggatcgct atgccttgtc gtcccagttg aaagcagcac atgctgtcga agccggtgta
     8821 ttttctgagg agatcatccc agtcaagatt tctgatgaag acgtgttatc tgaggatgaa
     8881 gcagttcgtg gaaatagtac attggaaaaa ctgggcacgt tacgtacagt attctcagaa
     8941 gaaggaactg taacagcagg aaatgcttcc ccgttgaatg acggtgcctc tgtggtgatc
     9001 cttgcatcca aagaatacgc agaaaataat aatctgcctt atttagcaac catcaaagaa
     9061 gtagcggagg tcggtattga cccaagtatc atgggaatcg ctccaatcaa agccattcaa
     9121 aaattgacag atagatcagg aatgaattta tcaacaatcg atctttttga gatcaatgaa
     9181 gcatttgcag cttcttctat cgtagttagc caagaactgc agttagatga agaaaaggtc
     9241 aatatatatg gaggagcaat cgcattaggc catccaattg gtgcaagcgg tgcacgtatc
     9301 ttgactacat tagcttacgg attgcttcgt gaacagaaga gatacggtat tgcttcatta
     9361 tgtattggtg gaggtttagg cttggcagtc ttgttagaag cgaatatgga gcaaacacat
     9421 aaggatgtgc aaaaaaaaaa gttttatcag cttacgccat ctgaacggcg cagtcaatta
     9481 atagaaaaaa atgtattgac acaagaaact gcgttgattt ttcaggaaca aacactttct
     9541 gaagagctct ccgatcacat gatcgaaaac caagtgagtg aagtggaaat cccaatgggg
     9601 atcgcgcaga attttcaaat caatggcaag aaaaaatgga ttccgatggc aacagaggaa
     9661 ccatctgtca ttgcagctgc aagtaatggt gcaaaaatct gtggaaatat ctgcgcagaa
     9721 acacctcagc ggctgatgcg agggcaaatc gttctatcag gaaaatcgga gtatcaagca
     9781 gtgatcaatg cagttaatca tcgaaaagaa gagctgattc tttgtgcaaa tgaaagttat
     9841 ccttcaatcg tgaaacgcgg gggcggtgtt caagatatct ctactcggga attcatgggg
     9901 agtttccatg catacttatc tattgatttt ttagtggatg taaaagatgc aatgggcgct
     9961 aacatgatca actcgattct tgagagtgtg gcaaacaagt tacgggagtg gttccctgaa
    10021 gaagagattc tattcagtat tttaagtaat tttgctacag aatcattggc ttctgcctgt
    10081 tgtgaaatcc cattcgaacg cttagggaga aacaaagaaa tcggtgaaca aattgccaaa
    10141 aaaatccaac aggcagggga atatgcgaag cttgaccctt accgtgcagc aacgcacaac
    10201 aaagggatca tgaatggtat agaagctgta gtggcagcta caggaaatga tacacgtgcg
    10261 gttagcgctt cgatccacgc ctacgcggca agaaatggat tataccaagg attaacagac
    10321 tggcagatca aaggggataa actagttgga aaactaacgg tgcctttggc tgttgccaca
    10381 gtaggcggag cttccaatat cctgccaaaa gcaaaagcat ctttagctat gttagatatt
    10441 gattcagcca aggaactagc acaagtaatt gctgcagtag ggttagctca aaacctggct
    10501 gctttgcgtg cattagttac agaaggaatc caaaaaggcc acatgggact tcaagctcgt
    10561 tcattagcga tctcaattgg ggctattggt gaggaaatcg agcaagttgc gaaaaaatta
    10621 cgtgaagcag aaaagatgaa ccagcagaca gctatacaaa tattggaaaa aatccgggaa
    10681 aaataaaaaa gcaatatctt tagaagaata aagcaagatt tacagttatt ttatttactg
    10741 cagatcttgc t
//
LOCUS       DAYYGS010000089        10695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000089 DAYYGS010000000
VERSION     DAYYGS010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.089"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(214..1062)
                     /locus_tag="ACHA44_002312"
     CDS             complement(214..1062)
                     /locus_tag="ACHA44_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8905566.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1226..2128
                     /gene="gnd"
                     /locus_tag="ACHA44_002313"
     CDS             1226..2128
                     /gene="gnd"
                     /locus_tag="ACHA44_002313"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HFM8905567.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            2130..3131
                     /locus_tag="ACHA44_002314"
     CDS             2130..3131
                     /locus_tag="ACHA44_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HFM8905568.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3128..3412
                     /locus_tag="ACHA44_002315"
     CDS             3128..3412
                     /locus_tag="ACHA44_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFM8905569.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3445..4242
                     /locus_tag="ACHA44_002316"
     CDS             3445..4242
                     /locus_tag="ACHA44_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8905570.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4256..5089
                     /locus_tag="ACHA44_002317"
     CDS             4256..5089
                     /locus_tag="ACHA44_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8905571.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5107..5964
                     /locus_tag="ACHA44_002318"
     CDS             5107..5964
                     /locus_tag="ACHA44_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8905572.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            5986..6495
                     /locus_tag="ACHA44_002319"
     CDS             5986..6495
                     /locus_tag="ACHA44_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFM8905573.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6532..6933
                     /locus_tag="ACHA44_002320"
     CDS             6532..6933
                     /locus_tag="ACHA44_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905574.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7045..7398
                     /locus_tag="ACHA44_002321"
                     /pseudo
     CDS             7045..7398
                     /locus_tag="ACHA44_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7432..7875
                     /locus_tag="ACHA44_002322"
     CDS             7432..7875
                     /locus_tag="ACHA44_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8905575.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7892..8977
                     /gene="uxuA"
                     /locus_tag="ACHA44_002323"
     CDS             7892..8977
                     /gene="uxuA"
                     /locus_tag="ACHA44_002323"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8905576.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8970..9683
                     /locus_tag="ACHA44_002324"
     CDS             8970..9683
                     /locus_tag="ACHA44_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8905577.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9890..10039)
                     /locus_tag="ACHA44_002325"
     CDS             complement(9890..10039)
                     /locus_tag="ACHA44_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905578.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10435..10635
                     /locus_tag="ACHA44_002326"
     CDS             10435..10635
                     /locus_tag="ACHA44_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFM8905579.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3568 a         1694 c         2315 g         3118 t
ORIGIN      
        1 attttttact actgtatact aactttgtta tcgcatctaa caagtaactc gaataatacc
       61 aatacaacct tctccaaaga tgcgatgaca tacgttctaa cccaccgact attgatgttc
      121 tgtaccaccc ttgcagaacc aaaaaagcca ctaccaaata tttggtagtg gctttttttg
      181 ttttatggat ttttcgcttt ttctgttatg gtgctattct ttgatttcag aaatagtatg
      241 tcgaacttta gcgatagaac gacgtgattc tttttcgttg taaaacatca aaatcacata
      301 tagagagtcc aaaatgctta gttgagaaat acgggaagct aaagcttcag atcggaattc
      361 tgtttcttca gaaatagaaa taaaaacgac atcgccaagt ttagcaaggg aagaattagc
      421 ttggctagta ataacgattg tttttgctcc agtttttctt acggtttcag caatatggat
      481 tgtttctttt gattgccctg tgtgagaaat caaaacagca cagtcttttt ccgtcaataa
      541 agaagcttcc atcaattgaa tatggtaatc cgtgctgtga gaagttgcga taggggaacg
      601 caaaaattta tgataagcgt cagttgctac aatctctgaa ccgccaacac cgaaaaaata
      661 gacacgttta gattcattga taatagtagc cgcttttttt agatcctttt gttgtaacaa
      721 cttttttgtg tcatttaaag ttgaaatact agactcgaac accttttgcg ccatagtgag
      781 ctcagtatct gttttctgaa tattttcgtg gatagaaata gcagatagat cattttcttg
      841 gatcaacatg gccattttaa aatctttaaa tccattataa ccaagttttt ttgtaaattg
      901 aaaaaaagta gagtcagcaa tgcccagttc acttgctaaa ttgctaatag gactatgact
      961 tactttgctt ggattttcta aaatataatc ggcgatagcc tgttcttttg ggctgaagtc
     1021 gttgtacatt gttttgattt gtagtcttat ggagacgctc atgagaattc ctcattttca
     1081 taataagatt atcatttttt ccgttctcat taatgttaaa gatacaagga gagtaggaaa
     1141 aaatcaaaca aaaaatattt ttgtaaaatc tatttacaaa aaaatttttt tgtataatga
     1201 gggtataaga aatggaggcg ctatcatgga aattggtgta attggtttgg gaaaaatggg
     1261 attgaattta gctttaaact taaaagagca ccatcacaaa gtacaaggac tcgacatttc
     1321 aagtacagct gttagtcagg caagagaaaa tgacattcct gcttatcaag agatctctga
     1381 atttctagct acttttgagc agaaaaaaat catctggtta atgcttcctg ctggagagat
     1441 aaccgaaaac atgttaagaa aactttttcc aaaactcgga ccaggagaca ttattattga
     1501 aggcggaaac tcttattata aagattcaat ccgtcgagca gaagagtttc agaaaaatga
     1561 aatcggttac ttggattgtg ggacttctgg aggaatcgaa ggagcacgac atcaagcctg
     1621 cttaatgatt ggaggagata aagaaacttt tctatccgtg gaacaattat tcaaagatgt
     1681 agcaattgaa agaggatatc tgtatgctgg tgcatccgga agcggacact tcttgaaaat
     1741 gatccataat ggaatcgaat atggaatgat gcaagcaatc ggtgaaggat tccaacttgt
     1801 acaaaaaagt gagtatgctt ttgacttgga acaagtcgca cgtgtttgga atcatggttc
     1861 ggtgattcgc ggctggctga tggaaatcat ggaagatcag ttaaacgaac atcccaattt
     1921 aagtaattat cggggaatcg tttctgcatc aggtgaagca aaatggacgg ttgaaacagc
     1981 gctagaaatg gctgttccag ttccagtaat tgcaactagt ttgttcatga ggaatctttc
     2041 acaggaagaa gatagttttt cagcaaaagt agtttcagca ctaagaaatg ggtttggcgg
     2101 acatgaaata gttgaaaagg gtgagtaaaa tgccagatta tcatatagca atcgttaatt
     2161 ccagcagttt cggaagagtc tttccacacc acttggaaag attaaagtca gttggagacg
     2221 ttcatttctt tactgttgat caagagattc caggaaaaga actagcggag ttacttaaag
     2281 gattcaatat cattattgca agtgttacgc cattttttcg acgagatttt tttgaaaaca
     2341 aagatgaact gctattgatt tcccgacatg gtattggtta caacaatatt gatttaaaag
     2401 cagcaaaaga acaccagaca atcgtatcta tcatacctgc tttaattgaa cgaaatgctg
     2461 tagcagaaaa taatataact aatttgttgt ccatgcttcg acgcacgaca caagcacaag
     2521 caagagtcaa ggacgatcga tgggaagatc gggcggagtt cgtcggtagg acgctgttta
     2581 acaaaacagt tggtgtgatt ggtgcaggaa ataccggaag cggagtaatc gaaattttgc
     2641 gtaacggttt tcgctgtgat gttctagctt atgatcctta caaatcagat ttatacatcg
     2701 aaagctttgg tgcaaaaaaa gtagaacttc aagaattact ggaacaatca gatatcattt
     2761 gtttatgtgc gaatttgaca gaagagaact atcacatgat ttctgataaa gaagtcgatc
     2821 agatgaaaga aaatgtttat ctatctaata gtgcaagagg cgctctagtt gatgaagaag
     2881 cgatgattaa tggtctgaaa agcggaaaga tcgcaggata tgccacagat gttttagaag
     2941 aagaaccggg acgtcaagat catccgtatt tagcgtttga acaagtagtg attacgccac
     3001 atatatcggc ctacacaagg gaatgtttag aagcaatggg tgaaaaatgt gtcagagatg
     3061 tcgaacagat cagtcagcac cttcttcctg aacgttctgt ccagacaaag agcaactata
     3121 taaaaggatg atgaagaaaa tggtgaacgg tttaaaagtg aaaacaggcc ctcaatttta
     3181 tttatatgaa gaaggtggta tttcaaaagt ttctgatctc ttgaaatcat acggcgcgaa
     3241 aagagtactg gtcacccacg gaacggtatc atgggaaaag gcgctaccta aattagtatt
     3301 tttgaatgac gaaacgattc aattttttta tcatcgttat tctggagaat gtagttatgc
     3361 tgaagccaga cgaatagcta caatcatcaa aaaaatgaaa tcgatttcgt agttggcgtt
     3421 ggcggaggaa aactgaccga tctagtgggt tatagtgcca atcttgcaag aatcgatttc
     3481 ggagtcatcc ctactttagc tagtaattgt gctccatgga cacctcttgc tgtcatgtat
     3541 aaggagaatg gacaagcaga agggaaaaca gaacactttt acagacaagc tgcttttttt
     3601 ataacagatc cccaattagt gattgattcc cctgttccat atttcattgc cgggttagca
     3661 gatacactag caaaatggta tgaatctgag ctcatcttag aacaagattt tttgcaagat
     3721 gaatcatttt tgaagctagc acaagatact gcgaagatct gcaaagaaga gattttgaat
     3781 cattctgcta aagcaatcca agatatggca caaagaaaac tgacatcgga gttcaaacag
     3841 ctttcagaga ttatctttgc tgttgctggt ttagttggag gattaggaga taaatacgca
     3901 agaaatgcgg cagcacatgc tatgcatgac gctatcagta aatatattcc ggaaagccat
     3961 cgttttctac atggagaaaa agtagcgtat gggattttct atcaactggc actagaaggt
     4021 cgctgggaaa aaatagacga gttatatcct ttgtatcggg aattagaact gccggtttct
     4081 ctagaacaaa tgggattatt tccgaaagat aaacagataa tagataaaat ggctgaattt
     4141 attgattcga aagagaaagt gcatttgatt cctatagagg taacacagaa atcactgata
     4201 cagtcaatga tggaactgga aggttatatc agaaagatct gaaggaggag gacacatgga
     4261 aacattaagt gtagtacaaa gtttgttgat tgctttatgg gtagccgcgg ttatgtcccg
     4321 ctggttaggt ggaggagcca cgttgacctt acgtttttct cctttgatga ctggattagt
     4381 cgtcgggttg atcatggggg aagttgcaca agcgatgatt gttacagccg ctttgcaaat
     4441 gatttatatg ggggtttttt ctccaggagg atcaatgcca gctgaaccat ctattgcagc
     4501 agctatcgca gtaccagtcg cattattagg ggatctgtcg ccagaagcag cgattgccgt
     4561 tgcggtgcca gtcggattgc tcggaagtta cttgtatcag tttcgttttt tcatcaatac
     4621 ttttttaggt aaatatacag acaaagctgt agaagatttg aatacaaaag ctatggcccg
     4681 atcaattatt ctttatccaa cggttgcctc atttttatta ttcgttccgc ttgttttcat
     4741 tgctttatat ttaggggcgc cattgatcgc tgatgtaatc aaagctttag agggaacagt
     4801 cgttatccac attcttgaag tagtaggtgg agggttagcg gcaatcggta tcgcgacaac
     4861 tgtatacgtt atcggtcgaa aagattatct ggtctttttc ttccttgctt attttatgag
     4921 tgttgttttg aaatctttag atattacgat ggttacttat gccatttttg ggattattat
     4981 cgctttgatc tttgtccagt cgcaaaaagg gaaacaagta cagcctgcca caagcaataa
     5041 caattcagca tcttttgatg acgaagatga ctatgatgac ggattttaat ttaggaggaa
     5101 tgggaaatgg ctgaaataat ggataatcaa acaggtgctc ctgaagaaat cacgaaaaaa
     5161 gatgtaacaa aagcctatct gagatggcat tttgcaaacg aaattcctca ctcttttgaa
     5221 cgttacttgg cgccatcact actatacgga atgatgccaa tcttgaaaaa actttataaa
     5281 gatgaagact ctttaaaagc agcatataaa cggcagttac tgttcttcaa cactcaatta
     5341 agctggggtg gcggtgtgat tactggccta atgtcttcta tggaacagca aagagcagaa
     5401 gaagagtatg aagaaaaaga gatcttgatg caggatgact tgatgtataa cacgaaagcg
     5461 ggattgatgg gggcactagc aggaatcgga gatgcaatcg attcaggaac cgtccaatat
     5521 atttttatcg caatcgcggt accttgggca caacagggaa gtcctttagg cgcacttttt
     5581 ccttttatct gctttgggtt gtatcaggtt ttactagggg tatttttcgc aagacaagct
     5641 ttcactatgg gccgtaatgc tacaggccta atgcaaaaca ctggtgttca gacagcgatt
     5701 gaaatgctat cagttttagg gttgtttatg atgggggtgc ttgcgggaaa ctatgtaaaa
     5761 gtagaatctt cattgaaatt tgcgatatcc ggacgtgagt ttgttatcca ggaaattctt
     5821 gatcaaatcg tgccagggat attaccttta gctgtcgtaa tgggtgtgta cttgttctat
     5881 acgaaaaaag gattgaaagt cacacaagca ttattatggc tgactggaat attaattgtg
     5941 ctagcaggaa tcggtatttt ataaaaagaa gggaacgtga ataagatggg tgtagtcaat
     6001 ttagcgcgtg tggatgagcg tttgatccat gggcaagtaa tggtaacgtt atctcaaaaa
     6061 agtggagtga attccatttt tgttgtagac gaagttgtag caaaagataa atttatgaga
     6121 gatctttaca aaaatgcagg aagccgtaca ggacaaaaaa caattgtcat tacaccagag
     6181 aaagctaaat tctattggga tgaatatcaa tttaaagaat acaattgtat tttgatcgca
     6241 aaaacagtcc aaacgattta cgacttagta aaacacggaa tccccatgga agaattgaat
     6301 attggaggga ttgctcaaaa aaatcctgat acagatcttt tagtgacgaa atctgtttat
     6361 ttaaataaat cagatgctga aaaattgaaa gaaatgaatg aacaatatgg cgtaaagaat
     6421 atttattttc aagccactcc atctgcacct aaaacaggat taaaagatgt attgaacaag
     6481 tttgatttgt cataaacaaa tgcttagaaa aaagataaag agggcgaaaa aatgaagaag
     6541 aacgagctgt ttcgtgattg ggaatttcgt tatcgctata tttaccgtaa gcgccgtaca
     6601 aaaaagagta aacagcgttt tctttcagca ttagtatcag atatttacag tatgagaaca
     6661 gatgtgacag tcattgctta cgatacgcta gcctaccgat caaaaaatat ttatgttgga
     6721 gatatcgaaa aagcagagaa agttatctgt acttattacg atacacccgt tcatgcatta
     6781 ggaagttatt tcatgtttga ttggaaagac caaaggaaaa aaacaatcta ctctatatta
     6841 ttgtccttca ttttgttgtt cagtttaggt tggtggggaa tgatgatata taacaaaaat
     6901 cctcatcacg tattcgatct gctatccgta taaacaggta tcactgtact agcatttggg
     6961 agctactttt tcttattggg aaaagcagct cgcggatggc ctagcagaca aacttttatt
     7021 cgaaatactt catccatatt aacgatgcta gaaatgatcc gtacgattga tgatccgagt
     7081 gtagcgtatg cgtttgttga tgaaggctgt tatggagaaa agggcttaga cagcgtgaga
     7141 tcgggtatga aaaaagaggc tattcttttt tatctagact ctgttggggc agatacaccg
     7201 ctacagtttt cgggaaatta tttttcaaat aaagaacagt gactgaaaca agttgataag
     7261 ttgaaagaaa aaaacgtcaa ttatattttt agtgcaagaa aaaagcaagc gcagttcttc
     7321 tatttgacca aaacagattt acgagggaaa acatttaatt ggcaaaatgc caatcagatt
     7381 attgctcttt ttcgataagt aaaaagtgca aaacaaggag tgagaagact aatgttggaa
     7441 atcatgatcg ttacacatgg taagttgagc gaaggactga aagatgcggc ggaagtaatt
     7501 gtcgggacat cgagccatat tgaaacaatg tgcttagagt ctggggagga catccaaaca
     7561 ttcggagaga aggtcaaaga aaaattaact atgatcaaca cagcagaacc catgctagta
     7621 tttgttgatc taattagcgc aagtccgtac aatcaagcgc tcttggcagt caatgaactg
     7681 actgaagaaa agaaagcagc cacttatgtc atagggggcg tgaatctgcc aatgttgtta
     7741 gaagcaatca atcatcagat gcttcacaca ccgatagaag aagcggtgga acaaattgtc
     7801 aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa aaaattagat
     7861 gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat ggtatggtga
     7921 aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa atgggataat
     7981 tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa ttgaggagtt
     8041 gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg tagcgatcca
     8101 cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact atatacgaac
     8161 aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca aaccaatttt
     8221 tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt ctcttttata
     8281 cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga tacacagcca
     8341 gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat tcgctcagct
     8401 ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga cttatttttt
     8461 agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc atccagatga
     8521 tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg acttgaagaa
     8581 aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag gatctttagg
     8641 ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc gcatccattt
     8701 tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa cggcacatcc
     8761 ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc aagaagggtt
     8821 cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg ctatgcctgg
     8881 atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat atgaggcgat
     8941 aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc atctttgata
     9001 tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta aaatcagcac
     9061 aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc caaacagaaa
     9121 tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat atcaaatggc
     9181 gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga gtatatacga
     9241 aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat acaatcgtgg
     9301 cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat ctatatgcct
     9361 tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa ccagagatct
     9421 ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta tttgaagact
     9481 cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc atcaaagatc
     9541 atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat gtaagtttat
     9601 atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa cgatttttag
     9661 aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt tatttattgt
     9721 cctgcacgac tgagggaaca gatgaaaaag aagtaatttt ttattttgaa gtatctacct
     9781 tatttgtagt gaacaattta gaacaaacac aaaacgcatc tactgttatc taccagtaga
     9841 tgcgtttaaa gagaagaacg agaatcgtcc atatgagggt ttccactcct tatgcagatt
     9901 tttttaattc ttgcactgct tgttcgatcg taacacctgt cgctacatga ttggtatttt
     9961 ctttgtcaaa aacgacaaat agattcaaat ctgtatccaa ggttactcga tattgtatat
    10021 ctttgttata gaatgtcata gccattctcc tttctttaaa tacttgctga aaggcaacga
    10081 acgcgttgat aacgaaattc catttataag tataccacca attgaaaggt tttcatagtt
    10141 aagaaatttt aaagtcaaaa gttgcagata aaagcaaata aacaagaaaa caaaaagtga
    10201 atcaatcgcg gttattcaac tttttcttcg attcgaagac aatagaagac aatgaatttt
    10261 atatttcatt gcattatcct tgtgttatta gaaaaagcat gatatattaa atgagtaatc
    10321 tattttgaaa cgtaatttga gcgatatatc tacatagata aaactccttt tgcctatcaa
    10381 atatttattg caacgaaaaa acgtacattg atacgtatca ggaggaattt atttatgaat
    10441 aacggtacag taaaatggtt caacgcagac aaaggttttg gctttatcac tggtgaagat
    10501 ggaaatgatg tatttgcaca tttttcagct atccaaggtg acggcttcaa aactttagaa
    10561 gaaggccaag cagtgtctta cgatgttgaa gatggtcaac gtggccctca agcaacaaac
    10621 attgttaaag cttaataaaa attgactttt aaccgtcttt tcgaaagaaa agacggtttt
    10681 tttgtacgaa aaaat
//
LOCUS       DAYYGS010000090        10624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000090 DAYYGS010000000
VERSION     DAYYGS010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.090"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            23..646
                     /locus_tag="ACHA44_002327"
     CDS             23..646
                     /locus_tag="ACHA44_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905580.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA44_002328"
     CDS             1096..2145
                     /gene="trpX"
                     /locus_tag="ACHA44_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HFM8905581.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="ACHA44_002329"
     CDS             2142..3023
                     /locus_tag="ACHA44_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905582.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="ACHA44_002330"
     CDS             3026..3796
                     /locus_tag="ACHA44_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905583.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA44_002331"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="ACHA44_002331"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFM8905584.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="ACHA44_002332"
     CDS             5107..6270
                     /locus_tag="ACHA44_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HFM8905585.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="ACHA44_002333"
     CDS             6387..6797
                     /locus_tag="ACHA44_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFM8905586.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="ACHA44_002334"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="ACHA44_002334"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFM8905587.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="ACHA44_002335"
     CDS             9370..9654
                     /locus_tag="ACHA44_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905588.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="ACHA44_002336"
     CDS             9814..10050
                     /locus_tag="ACHA44_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8905589.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="ACHA44_002337"
     CDS             10047..10445
                     /locus_tag="ACHA44_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFM8905590.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3710 a         1771 c         2248 g         2895 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttag
//
LOCUS       DAYYGS010000091        10603 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000091 DAYYGS010000000
VERSION     DAYYGS010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.091"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            70..552
                     /locus_tag="ACHA44_002338"
     CDS             70..552
                     /locus_tag="ACHA44_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905591.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            749..1639
                     /locus_tag="ACHA44_002339"
     CDS             749..1639
                     /locus_tag="ACHA44_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HFM8905592.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            1965..4439
                     /locus_tag="ACHA44_002340"
     CDS             1965..4439
                     /locus_tag="ACHA44_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HFM8905593.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4542..6278)
                     /locus_tag="ACHA44_002341"
     CDS             complement(4542..6278)
                     /locus_tag="ACHA44_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8905594.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6275..6979)
                     /locus_tag="ACHA44_002342"
     CDS             complement(6275..6979)
                     /locus_tag="ACHA44_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8905595.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7110..7613
                     /locus_tag="ACHA44_002343"
     CDS             7110..7613
                     /locus_tag="ACHA44_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HFM8905596.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7573..7911
                     /locus_tag="ACHA44_002344"
     CDS             7573..7911
                     /locus_tag="ACHA44_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HFM8905597.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7932..9350
                     /gene="ffh"
                     /locus_tag="ACHA44_002345"
     CDS             7932..9350
                     /gene="ffh"
                     /locus_tag="ACHA44_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFM8905598.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9462..10040
                     /locus_tag="ACHA44_002346"
     CDS             9462..10040
                     /locus_tag="ACHA44_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFM8905599.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10044..10565
                     /locus_tag="ACHA44_002347"
     CDS             10044..10565
                     /locus_tag="ACHA44_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HFM8905600.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
BASE COUNT         3462 a         1915 c         2239 g         2987 t
ORIGIN      
        1 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
       61 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
      121 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
      181 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
      241 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
      301 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
      361 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
      421 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
      481 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
      541 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
      601 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
      661 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
      721 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
      781 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
      841 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
      901 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
      961 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     1021 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     1081 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     1141 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     1201 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     1261 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     1321 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     1381 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     1441 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     1501 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     1561 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     1621 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     1681 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     1741 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     1801 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     1861 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     1921 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     1981 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     2041 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     2101 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     2161 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     2221 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     2281 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     2341 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     2401 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     2461 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     2521 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     2581 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     2641 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     2701 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     2761 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     2821 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     2881 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     2941 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     3001 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     3061 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     3121 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     3181 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     3241 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     3301 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     3361 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     3421 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     3481 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     3541 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     3601 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     3661 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     3721 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     3781 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     3841 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     3901 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     3961 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     4021 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     4081 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     4141 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     4201 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     4261 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     4321 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     4381 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     4441 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     4501 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     4561 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     4621 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     4681 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     4741 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     4801 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     4861 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     4921 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     4981 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     5041 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     5101 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     5161 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     5221 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     5281 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     5341 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     5401 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     5461 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     5521 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     5581 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     5641 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     5701 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     5761 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     5821 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     5881 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     5941 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     6001 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     6061 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     6121 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     6181 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     6241 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     6301 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     6361 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     6421 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     6481 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     6541 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     6601 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     6661 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     6721 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     6781 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     6841 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     6901 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     6961 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     7021 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     7081 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     7141 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     7201 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     7261 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     7321 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     7381 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     7441 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     7501 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     7561 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     7621 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     7681 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     7741 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     7801 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     7861 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     7921 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     7981 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     8041 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     8101 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     8161 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     8221 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     8281 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     8341 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     8401 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     8461 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     8521 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     8581 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     8641 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     8701 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     8761 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
     8821 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
     8881 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
     8941 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
     9001 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
     9061 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
     9121 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
     9181 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
     9241 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
     9301 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
     9361 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
     9421 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
     9481 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
     9541 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
     9601 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
     9661 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
     9721 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
     9781 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
     9841 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
     9901 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
     9961 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    10021 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    10081 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    10141 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    10201 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    10261 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    10321 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    10381 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    10441 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    10501 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    10561 agtagaagta aataaaataa aaaaggcaac tatatgcaga aat
//
LOCUS       DAYYGS010000092        10312 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000092 DAYYGS010000000
VERSION     DAYYGS010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.092"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(49..1323)
                     /locus_tag="ACHA44_002348"
     CDS             complement(49..1323)
                     /locus_tag="ACHA44_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HFM8905601.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1524..2738
                     /locus_tag="ACHA44_002349"
                     /pseudo
     CDS             1524..2738
                     /locus_tag="ACHA44_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2917..3252)
                     /locus_tag="ACHA44_002350"
     CDS             complement(2917..3252)
                     /locus_tag="ACHA44_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFM8905602.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(3236..3499)
                     /locus_tag="ACHA44_002351"
     CDS             complement(3236..3499)
                     /locus_tag="ACHA44_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFM8905603.1"
                     /translation="MAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQGKI
                     PFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(3959..4762)
                     /locus_tag="ACHA44_002352"
     CDS             complement(3959..4762)
                     /locus_tag="ACHA44_002352"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFM8905604.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(4740..5864)
                     /locus_tag="ACHA44_002353"
     CDS             complement(4740..5864)
                     /locus_tag="ACHA44_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905605.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(5880..6695)
                     /locus_tag="ACHA44_002354"
     CDS             complement(5880..6695)
                     /locus_tag="ACHA44_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905606.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(6685..7557)
                     /locus_tag="ACHA44_002355"
     CDS             complement(6685..7557)
                     /locus_tag="ACHA44_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFM8905607.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(7642..8931)
                     /locus_tag="ACHA44_002356"
     CDS             complement(7642..8931)
                     /locus_tag="ACHA44_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905608.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(9075..9587)
                     /locus_tag="ACHA44_002357"
     CDS             complement(9075..9587)
                     /locus_tag="ACHA44_002357"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905609.1"
                     /translation="MTINCDLKEEGLEMAVFSLAQTYQIWPQIVLSGYVQLSWAAIWG
                     KHIFYNIENQFEGKKLGDITEADLIDTLQKLAYQSVQIVNIHEGWLTKLAREVGQQLG
                     LTFSVWTVNELETIEYLARCGVYNITSRCAWAYLWKKGAQNKLFYNQNRGVKRFGDGI
                     HDHMGALFLA"
     gene            complement(9654..9803)
                     /locus_tag="ACHA44_002358"
     CDS             complement(9654..9803)
                     /locus_tag="ACHA44_002358"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFM8905610.1"
                     /translation="MTMITAHSGSDNTEENSWAFLRFCLDRQLCFEVDVRQQTDGTLI
                     LAHDR"
BASE COUNT         3164 a         2158 c         1710 g         3280 t
ORIGIN      
        1 ataaaactta gtccgatatg cggcgcgttc tgtgcgccgc atattttttt agttttcaaa
       61 gatcagacct tgttgtaaat agatccgtgc ccgaaagtta taaaagttgc gataaccaaa
      121 cgcaattcgt ttaatcagct tgatcttgtt attcagcccc tcagtgacgc cgtttgagtg
      181 ggacacctta aaggcatttg aaatacccga ttggtagcaa tggaagatcg tgaatttctt
      241 tttaaacgac tgaggtaacg tagatgaaat tgttttaagg gtttcttgaa agccttccgt
      301 acctcgttta acgaaataat agcgtaataa ttgacaaaaa tgatacgcag ttcgcaattc
      361 ttcgttgtag cttaacagct catcaacgat ttctgtttga ctaattggtc gtttaaacag
      421 tcgatgataa tgccgtttga gtggctccca tgtcgtggaa tctttgagta agagtttcca
      481 atatcgcttg agccgacggt acttcttctg attttcagag tgtgaggttc gaaaacgatt
      541 catgatctgg acacgtaatt gattgaagct gcgattgatg tgttggacaa tatgaaagcg
      601 gtcagttacg atctccgcat ttggaaagac tgttttaagc aactgacaat agctggcatt
      661 catgtccatc actaggtact tgacctttaa acgggctttt ctaggaaagt gcaggaagta
      721 ttgagttagt ttgtaaagtc tgcgatcttc aaggatcgta aatagctgat gggttgtccc
      781 atcaacacaa ataaaactca tggaaccaga aacagacttc attgatttga actcatccat
      841 acacaacacc gtcggaagga agttcatacg tgactggtaa tgcttcgttg cttcacgcaa
      901 gatccgtgtc accgtcccat ccgagacgaa gtacttctta gcgattagtt tacgtgattg
      961 attttcgtac agttccatga gaattgcctg cttcagttcc tttgttagtt gatgatgttc
     1021 atcaactaag tccgttttcg cagaaaaggt ccttccacaa tcatggcaga gaaaacgggt
     1081 acgttttaat tctaaaatag ttaacttttc tttgactttc aatatctgag tgcgtgtcgt
     1141 ataggaaccg ttgcggataa tacgaaccga tcgacagtgt tcacaatagg aaggagagtc
     1201 atccaatttt cctttaataa actgcgcttg aacgccttta tattcttttt caatcagcca
     1261 ttcttcatca aaaaataaat gtttatctgt taatccgagt aattttctag tatgattatc
     1321 cattgagaga ctcctttggt aggatgacgt tttggtcgac taaattttac caaaagtgag
     1381 tctctttttg agttgaaacg aagaaaaagt gttagtagaa aatcgccttc gattttccac
     1441 caacacaaaa tattatagaa gcaaaatcaa ttctacctta gatgttctta tatcactcta
     1501 tcagtcctat ttgacaaaga gccaagaaaa agacgttggt cgtcgatgct gttctctcgc
     1561 ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac gggaaagcaa
     1621 ttatagtgaa aaatgggaag aaggaaacga ttgtccgttt tgaacaatac aatcatatgc
     1681 ctttggttac gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgt acccattgga
     1741 cggctcaaag ttattttgtc cgactcagcc attcaatcgc agatcatgtt cgatataaaa
     1801 ttgcttcttt actgactgaa aaagtatctt tgtcttttat tgcgaaaagt tgtcaggtat
     1861 ctttgactac cgttactcgt acattgaaag agtttaaaag ctatttacca aagccttcta
     1921 agaagattct ccccagagta ttgatggttg atgaatttcg ttcgcacgct tccatagaag
     1981 ataaaatgag ctttatttgc gcagatggtg aaacaggaaa cttaattgct gtcttgccta
     2041 cgcgtaaatt acctcgatta acaagctatt tcttaggctg caccaatcca gaagaagtag
     2101 aattcttagt cacagacatg aacgccgctt acttccagct taccaaacgt gttctgccaa
     2161 atgcgaaagt agtgattgat cggtttcata ttgtcaaaca catgaatcag gcactcaatg
     2221 agttgcgtat ccgtgaaatg aatgagcttc gtaaagcagg acagaaaagt caggcagaaa
     2281 aactgaaaaa gaactggcgc tttctgctaa aaaatcgtgc aagcatcaac cattatgagt
     2341 acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tctcactgaa gcaatgatga
     2401 ttgatcgatt gcttgagttt tctgcgcctc taaaggaggc gtatcccttt tttcatgaat
     2461 tagtcgaagc ctttcgagac aaagaccctg acttattttt ctccttattg gcagaacttc
     2521 ccgaaacgtt agatgacggc tttcgggaga agcttcaaaa ccttctgacc tatgaagaag
     2581 gtatcaccag cgcaatgatc tatccttatt ccaatggaaa aattgaagcg aagaataccc
     2641 acataaagac aatgaaacga gtatcctacg gatttaaatc attcgagaac atgagaatta
     2701 gaatcttttt gatcaatcaa ttaatcaatg taagataaca aaaaatctga accagaatgt
     2761 ggttcattct gacccggatt tgattttgca caactcatca gtccttattg acaaagagcc
     2821 aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta caacccatca
     2881 gtccttattg acaaagagcc cttcttttta gtattattat aattcttcca tgcgttcttc
     2941 cagtcgctgt aaatcgcttg gacttaattc cccaattttt ttccatttga cctttttgtt
     3001 gtttttgggt aattcgttta gagttcctac gtcaatccaa gattcttttt ttagtcctgc
     3061 ctgtttccaa tcccttattt caacgtattt cagctttatt ttatcggatt tagtatgata
     3121 ttttgaagta attgacagaa acgaaattgt gtccagtcca ttttttagaa tatagacagg
     3181 ccggcgttta ccgccctctg cagctgtgaa agcaacgtag acggttgcta tatcattagt
     3241 attcatcttc attgaaccat tcttcaattt tttcgtcagt gtctagtttt tggacaggca
     3301 atttttccgc taatctttga atattatgga ttcgtttact tttttccgac aattcaatag
     3361 aaaatgggat ttttccttgc tcaacaattt gcttataaaa aacggtgacg gctgttttgg
     3421 ggctcatgcc tagttcatca ataatgcttt ctgcttcatt tttcaatttt tcgtccatag
     3481 aaatttgtaa cttagccatt ttctccacct ctttctgaca aacacattat atcacggaaa
     3541 gagttcaaat ggaattaata caactacttt tgaactcctt gaaatgcagt gtatttatct
     3601 aaatataagg aaaggatata gtgctaaatg gccttgatag atagaaaagt ttcaaaatcc
     3661 gccacagcga taattgaagc gaacactcaa cgacacacag aacaggaaaa gactcctaaa
     3721 agaaccaaat taactaggac gtgtctgaaa actccacaaa gatgtggtat gctctggtaa
     3781 aaaacgaatg gcacaaccac gaagaccgat atggttcttg gaaaactttc tccacccttt
     3841 ttgtaattgg agggatagtg gtttattgat agcgattttc caagttcttc aagtggaacc
     3901 agacattgag caccacggct aaaaagtgaa tgaagtcttt atttttaatc aataattttt
     3961 aatgattacc agtaattggt tttttcatgc gccctatttc tatttgatgt ggagttaaat
     4021 aatgctgata agtaaaaatt tttccgtcaa ttaaactgcc actagtgaat cctgttctat
     4081 tggtaaaaag agaatagtct gggtattcat ctacaccaaa tgataaagat tcagccataa
     4141 actgtttttt accagcaaat tcagcactgc taccttgatg gatatgtcct acaaaaatac
     4201 ctaaaatatc actttcggca atcaattcag caaaaccttc cggaacttca gttacaatag
     4261 atggatcttc ccacgacaat ggatgatgaa ccaataaaat cgttcccttt tcagcaggtg
     4321 tacccaacca ctcttgtaat tgaatcatct gtcctatcga tatttttcct tcgtgctggg
     4381 tatcttgagc agaatccaag gaaataattc gtaacccatt ttgattcgta caatggatat
     4441 actctcccgt ttcattgctt tcaacactca ttccttcaaa gaaaacactg cgacgatcat
     4501 gattcccacg acaaaaatag taaggtgtgg ttgggaaata ttgatccaac attttccgta
     4561 agacttgata atcagaaatc cccccttcat gtactaaatc acctgttaat agaataaaat
     4621 cgtacactgt taacgagagt tcttgtaaca atttttccaa tgccaactgt ggatttttaa
     4681 ttgttggaaa tagttcagca gtaaacaagt tagtttgata gttttctcta aaatggatat
     4741 cagagagatg caaaaagcgc atggctattc cctctttctt caataaaata atcaggatat
     4801 cctaaatttt cttgcgtctg ataatcaaaa aaatgcattc tttcaggttc tatgcggaga
     4861 ttgactgcat caccaatctg ccaagtaggt ttttcattaa ggacaatcat ttctatacca
     4921 tcgactttaa cggtgtacgc ataactttga cctaatagct cacgatactt cacttgaccg
     4981 tgtaaatcgc ttgtttcttt tgaaaaggtt acgtgttctg gtcttatgcc taaatacaat
     5041 gagccactaa cctgaagaag ttgtttttgc caagctggat tcaatgttaa aaattgactg
     5101 gctaccttta atgttccttg atcataagta actggcataa tattcatgcc tggcgaacca
     5161 ataaaatttg cagtaaaagt atttttaggt ttcgtataaa catttttcgg cgtatcaatc
     5221 atctgaattc taccttcatt caataaaaca atccggtcag ctaacgtcat cgcttcaatt
     5281 tgatcatgag tgacgtagac aaaagtttgt ttataacgtt gatgaatttt taccaattct
     5341 tttcgtgcat ccaatcgtaa acgcacatcc aaatttgata acggttcatc taataaaaaa
     5401 taattactcc gtttaaccac cgcacgagcc aatgctacac gctgccgttg ccctcctgac
     5461 aaatctttag gaagtcgatc ttggtatggc gttaaaccta acatcttcaa agcttcttcc
     5521 aaacggtgct gaatgtcagc tgtcttaatt ttattcgccc ttaatgcaaa aataatattc
     5581 tcctttacag tcatgtgagg atataaagca taattttgaa aaaccattgc gatctctcgt
     5641 tctccacaag gtagcttatt tgctagttgt gtacctaaaa aaagctctcc actcgtaatt
     5701 ttttccaaac cagcaatcat tctcagcaaa gtagattttc ctgaaccaga tggtcccagt
     5761 aacacaattc gttctccagc ggccacagct aaatcaattt cttttaaaat ggtatgttct
     5821 ccatattttt ttccgatatt tcttaattca agcgtttctc ccatgatttg tcctccaatt
     5881 tatttaatac ctgcagaaac aaaagtcccg ataatatatt tttgaaaaat tgcatacaag
     5941 actaacggta caaacattgt aattaaacta atcgccattg ctacggtaaa atttgtccca
     6001 ccttcagaac taatataggt ctgcatcgcc aacggcaaag tataattatc tactgtccgt
     6061 aaaattaata ccggccaaac atactcattc caagaggtaa taaaaaacca aataccggta
     6121 gaagtaatcg catgtctagt aagaggaaaa acaattttac tcatgacggt tgtttgtttg
     6181 atgttatcca atgccgccac atctagaagg ctcactggaa tttcccgcat tgtttgcgtc
     6241 aataaaaaaa tgcccatcgc atcagcaaat tgtggtatta tcacacccca agtagtatca
     6301 attaaattaa gttttgcaat caacaaataa ttaggaatca tcgcaacagt aaaaggaatg
     6361 aacatcgtac caatgatgat aaagtaccag attttcttcc ctttgattcg gcaataagta
     6421 aaactatatg ccgcaaaaaa agctacgcct aacttaacaa ccgttataat tgtagcaata
     6481 aaaaaagtat ttctcgtaat tttgataatt ggaagatttt cgaacaaata gcaaaaattt
     6541 tccaaggtaa acggctgtgg tatcaaacta gtaatcgtct gataggcatc tttcaatggc
     6601 ttaaaagaat tggccataat aaacaataat ggatagataa taatgattat gatgaacata
     6661 aataagagat gtggcaacca gcgtttaatt ttcatagtaa accccctttt ccacaaattt
     6721 aaatattacc cctaagaaaa cagcaaatat taataaagtt aaaactgaaa ttgctgaaga
     6781 ttctccgact ttatacattt caaaagcctt ttgataagca taatagatca cgtttgcact
     6841 accattattc ggtccaccac gagtgatgat cttaatcgga gtaaacacat actgcagacc
     6901 ttgaacaatg gtcatcacca ataaatatag tcctgtcgaa ctactcatag gcaacacaat
     6961 tgtaaaaaaa atgcgccaga tgggtacacg atccaactta gctgcttcga tcatctcttg
     7021 atcaattccc acaattgctg caaaaatgac aatgaaatta taaccaaaaa ctttccaagc
     7081 agtaatacct gcaatcacag caattaccca accttgtaaa ttcatccaca taggcagtct
     7141 taaaccaaaa tttcccaaca agacagcaat tggtcccgat accggattta atatccatgt
     7201 aaaaaccatc cccgccacta ccaaagaaat cacagctgga ataaaaaata atggtttata
     7261 caaattctgc cctttttcta taactattcc taataaaaag gatgtaatat atgggaacac
     7321 acagctcagc aagacaaata gaacggtata ggacaaagta ttcttcaaca ctttatgaaa
     7381 gactggatca gtcagcacct ttacatagtt ggcaaagcca acaaattctt tagtgggggc
     7441 caccatattc cagtcaaaaa aactcaaata gatcgtataa atcaatggat aaaaaacaaa
     7501 cacgcccaac aaaataattg ctggcgccag atacaaataa cctacatagc gcttcatttc
     7561 cgacagcctc ctcatcctta tgtcaaaagc agacgacctt gtttttcctt tcttacaggc
     7621 gctgtcgtct gcttgctttg cttatttgag taactcatta atttcctttt gagtagcttt
     7681 catcgtttca ttgacggaat tacctgataa aattttatcc cgcatatcca acaatttttg
     7741 ttcagcttct aatccagcat ctccaggaaa tgaactccaa ctgaccatct tatccatctg
     7801 atcaattgct ggtatcatca tctgattttc agctaagaaa gcttttaatc cattttgggc
     7861 gtctgcaaca tctttacgtg gtggtaaata accagtacct tctgtccatt tagctaaaga
     7921 ttcaacacta taaagatatt ttaagaaatc ccaagttgct tcctttttct tatccgtttg
     7981 tgatgtaaca gctaacattg cgccaccagc aggtagttta acttttttcc cttcccaact
     8041 tggtgactca actgctttag cagtaaaagt actatttttt tgaatgttgt ccctttgagc
     8101 aatagtggta tagaccatcg cgacgttacc atcaataaag ctttgcacac cttgttccca
     8161 cgtagtgtgt aatgccgtct ggtcttttac catcctttga tataattcat aagcagcgat
     8221 accatcttta gaagcaaagg tagctttgcc atcttttatt acacttgccc cattactttc
     8281 taaaattgct tgttgtgccc aattatcagc aggttcttga atatacacgc catactttcc
     8341 agttttttct tttattatct tagaaaaagt ttgaacttct tcccaagttt ttggaccatt
     8401 ttcatccaag cccgctgctt ttaatatatc ttgatttaaa tataaaaccg gattactaat
     8461 ggagtaagga atacccactt gtttaccatc tttttcagac agtgccatga tattgtctaa
     8521 aaaatgatct ttaaaataat cagcattatc cggatccact tcttctacta aagattgagg
     8581 attagtatat ttgaaatttt ctgcaaaata atctttaaaa gcccatccca cttgtactac
     8641 gtccggtatt tctttggagg caacagaagc ttgtaaattt tgcatcaagc ctttatacat
     8701 atcaggatta tattttgcta caaccttcac tttgtcttga gattgattgt atttatctac
     8761 taattcattc actgtttttc cacctagggt atccgcatta acatgccaat actctatcgt
     8821 tacacgaccc tccgattttt ttgttgtctt ggtattccca ctaccacaag ctgccaaaga
     8881 gatgacactt aaaacagaca caacccctat aacaagattt ttctttaaca taacattaac
     8941 ttcctcctaa tttctttatg gccatttttc taatcaagaa atattatgaa ctaccaatgt
     9001 aaatttctag ttaattttct gtaaataatt cgaaatttgt gcatttcggg gtacagggtc
     9061 actggacata tccatcaagc taagaataaa gcccccatat ggtcatgtat cccatctcca
     9121 aatctcttaa ctcctctgtt ttgattataa aatagtttat tctgtgctcc tttcttccat
     9181 aaataggccc atgcacaacg tgaggttata ttataaacac cacagcgcgc aaggtactca
     9241 atcgtttcta gttcattgac tgtccataca gaaaatgtca atcctagctg ctgccccact
     9301 tcacgggcca acttagttaa ccatccttca tgaatattga cgatttgaac gctttgatag
     9361 gctaatttct gtaaggtatc gatcaaatct gcttcggtaa tatccccaag ttttttccct
     9421 tcgaactgat tttcaatatt ataaaatata tgctttcccc aaatcgcagc ccaagataat
     9481 tgtacatagc cactaagaac aatttgtggc cagatttgat aggtttgtgc cagcgaaaaa
     9541 acggccattt ccaatccctc ttcttttaaa tcacagttaa tggtcatggt tgccttctca
     9601 ttggccactg cttgaaacac ctgctcaagg gttaccgaat ctgggacaac ttcttacctg
     9661 tcatgggcta gtattagcgt accatctgtt tgctgacgca catctacttc aaaacaaagc
     9721 tggcgatcca aacaaaagcg aagaaatgcc cagctattct cctccgtatt gtcactacca
     9781 gagtgtgcag tgatcatggt cattttgacc cctcctttaa ttccgtgatt ttctcccaca
     9841 cgtccactat accgatgaag cgttaatttc ttgttaattt cttgtaaatt ccctgcgttt
     9901 tttcagtaaa ctagttgtaa atcttgcatg ctccttccta cgaactcatg aatcgtcgat
     9961 tttgcccttc tagctagttt caccaattaa gtagttctac attccccgct tttaacctac
    10021 tacacctttt tttgttttca gctattcctt cttcttgctg aaagtccttg aggttctttc
    10081 ttacaggata ccatcaaggt gttcaatggc ttattcccat tgaatgaccg agacttacta
    10141 tcagatttct gctcgttgaa ccaagtcttt gatacaggat ttcttccgtc atcttctcac
    10201 aaataccacc ttgcgtttct ctagcgattg gtcgatgtgc accctcccag tggactgtca
    10261 ctaccccttt ttgctatgcc tagcccacaa aaaaagctgt cctcacagac ag
//
LOCUS       DAYYGS010000093        10001 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000093 DAYYGS010000000
VERSION     DAYYGS010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.093"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(162..368)
                     /locus_tag="ACHA44_002359"
     CDS             complement(162..368)
                     /locus_tag="ACHA44_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905611.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(384..998)
                     /locus_tag="ACHA44_002360"
     CDS             complement(384..998)
                     /locus_tag="ACHA44_002360"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFM8905612.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(1145..1828)
                     /locus_tag="ACHA44_002361"
     CDS             complement(1145..1828)
                     /locus_tag="ACHA44_002361"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8905613.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1937..2395)
                     /locus_tag="ACHA44_002362"
     CDS             complement(1937..2395)
                     /locus_tag="ACHA44_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFM8905614.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2479..4242)
                     /locus_tag="ACHA44_002363"
     CDS             complement(2479..4242)
                     /locus_tag="ACHA44_002363"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFM8905615.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4463..5923)
                     /locus_tag="ACHA44_002364"
     CDS             complement(4463..5923)
                     /locus_tag="ACHA44_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905616.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(6131..7057)
                     /gene="glsA"
                     /locus_tag="ACHA44_002365"
     CDS             complement(6131..7057)
                     /gene="glsA"
                     /locus_tag="ACHA44_002365"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFM8905617.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(7080..8507)
                     /locus_tag="ACHA44_002366"
     CDS             complement(7080..8507)
                     /locus_tag="ACHA44_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8905618.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8538..9752)
                     /locus_tag="ACHA44_002367"
     CDS             complement(8538..9752)
                     /locus_tag="ACHA44_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFM8905619.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         2975 a         2058 c         1649 g         3319 t
ORIGIN      
        1 gaaatagtga aatttaacaa tctttaatat tttttagaaa aaaataacaa ataagttgaa
       61 agaatcccgt tgcaaattct ttcagttgca ccaggattct ttgataattt tttagtttta
      121 tgtgtttttt tatatgtttt ttcacttttc ggaataaata tttatttgtt aaagtaatta
      181 aacaatccgc taatcgtttg atctggcatt tctatttcct taccatctaa agtaacagta
      241 gtaacgacaa aatggtcttg caatggcgga acttctaagt actgcccttg cagaaaatca
      301 ttcactgttt catattcttt tttcatttct tttgaatctg tcgatggttt atagcttatt
      361 tttaacataa ctagtgctcc tttttattcg ggacgtgcaa tcgtgaaaat ttctcctatt
      421 gctgcataat catttcctgc caaacggctt acagcattca attttcttgg atcgatcctt
      481 cctgtcttat catcatagac tgtttcattt aaatgatatt tctgtacttc tagcaaaaat
      541 aaatcagctg ttactttatt tttattccgt atctcgacat gttcatataa ggatgtttca
      601 aaacgaactt ttgcttctgt caatgctggt actgaaatga ttactgaatc agttaaagtc
      661 aagtcagtca ggtacagttc gctttcctca ttaggcagat tggctgctgt tttatttact
      721 tcttctacgt tgtcctcatc aaggatatgg ataacagctt ctcttgtctc aagaatattc
      781 tttgctgtgt cttttactgc atcttgtttt ctctggatcg ccaaggataa gatgggtcga
      841 ttcgaactga cgatattgaa gtaactgaag ggtgcgatat tcactatatc ttttgctgat
      901 ttagtcgtta ctactgctac tggtctaggg atgactgaac caatcagcaa cttgtaattt
      961 tcacgttctg tcaattctga aggactccgt tcaaacatgg tttcgccttc tttctataaa
     1021 attcgttaaa aagcagatct tgaagacaaa atgaaaagta atgctttttt catcttctta
     1081 gaatctgctt taaactatct atcaattttt cttttttgtc aagaatctgt gccgtgcgtg
     1141 ttttttagtt atttgcttta cttgcggctt cgacatatct tttagtgatc aattgcggac
     1201 gggtgataat agatccaact acgactgaaa aagcacctaa ttcatatgct ttctctactt
     1261 gttcaggaga gtcagtatgt ccttccataa ttactgggat atcgacttgt tctatgattt
     1321 gattcaataa atcaaaatca ggtgtctgcg tctttcgtga atattctgta tagcctgcta
     1381 aagtcgtaga caaaagatca aaaccatctt cttgcgcttg aagcgcttcc tcaaaagtcg
     1441 aaatatctgc catagctaac cgaccgtgtt cgtgaatcat ttggacaagt tcagcagttg
     1501 ttttttctgt cggacgttta cgagaagttg catccagtgc aatgatttct gcttgcgtct
     1561 ccaaaagttc ttccacttct ttttctgtcg cagtaataaa cacttctgac ccatcgtaat
     1621 ctcttttgat aatccctatt accggtaaat caacgattcg cttgatttca ttgatatctt
     1681 ctgtcgtatt tgctcgaata ccaactgctc cgccttcttt tgcagccaaa gccattctcc
     1741 ccataataaa agaactgtgt agcggctcgt tttccaaagc ttgacaagaa acgacgactc
     1801 cgcctttgat ttgttccagt gtatccacaa tatctcctcc agtaatgtta tataaaaata
     1861 cagcttcata taaaaatgta acccttttct attcaggaga caataatgag ctatttcttt
     1921 cagcagacct ttcttactaa aagaaaatta cttcaaattc ttcgtatacg accagcttct
     1981 cttctgtaaa ttctatgttc aacgcttctt tataagcatg tttaaaaaac tcagcatgga
     2041 ctttccgcca ataagctaat gtccgatccc cttctccttc tttccaagca tgctctttcg
     2101 tgacttcact aaaaggcaag atggtcacat ttatattttt gatgatacaa actggttcat
     2161 tttttccatt caggaccacg tctaattgac caacttttgg taaaggctca ttctcttgtt
     2221 tatacagatc gtaaccactt gatgtaccag tctttatctt ttttacaact agttcgctca
     2281 actcgtctgc cattttttct gtatccccaa atgaaaagaa acctaatttt tctgattgat
     2341 aactaggata tttttcttgg aactttttga catattcttc tacttgctga tacataacat
     2401 ccaccccttt gctttttata atgaaaaaac catccagata ctagtataaa caattatctg
     2461 gatggtgagc ataatgaatt attgaatatt caagacacgc agactatctt caacgatttg
     2521 ttttggtgtt aaatgataac gttcataaag ctgccctaca ggaacgctat ccgtgaattc
     2581 tttctctgca ccaaaattta atacgttcat tgaagaatca ccataataac gactgatatt
     2641 ttcgccaaat cctcctgaca aactaccatc ttctaatgtg atgactaatt cgtgtgtttc
     2701 ttttaattta tctaatgttt ctacgtcatt ttctgtgatc gttcctggat tgatcaacgt
     2761 tgcttcgatt cctttttttc taaaagcttt tactgtttct tttcctaatt caagaaaacc
     2821 gcctaaagct aaaattgcta cttttcctcc attatttact gtttgatagg ctggaatact
     2881 ataatcattt gcgatagctg taccatgttc tacaccatgt gtaggaattc ggattgctac
     2941 aggctgcgct gtttgttcga acgcccaatc tagcatagag atcatatcct cttttgttgc
     3001 tggagcaaga tatttgatgt tcgggatact tgataagtaa ggaatgtcaa atgttccttg
     3061 gtgtgttcgg tcactagctg agatcgttcc gccaccgact aaaataatca ctggattctc
     3121 gttgatcgct aaatcatgtg ataattgatc atatgcccgt tgcaagaaag tacttgattg
     3181 gaagacgact ggacgtactc cctgagaagc caaagcagaa gcgaaagtga tagagtgctg
     3241 ttcagcgatc cccacgtcaa aataatgatc tggatgtttt tcttggaatg ttttcagtcc
     3301 aaaggcgcct ggtatcgctg cattgactgc ggcaatagga gctgtacttg tttgcatttt
     3361 cttatccaaa taatccatga tgatcatatt aaatgtttct ccagaagtta cttgctttgc
     3421 ctccccggtg gacagatcaa atggtacatg ccaatgatat ttcattttat tatcgattgc
     3481 cggctgataa ccgcgacctt tttccgtatg gacgtgaagt acaatcggat gatcaatatc
     3541 ttttacttct tcaaataaac ggatcaccgt ttccagatcg tttccattat ccacgtaacg
     3601 ataatcaaat cccattgctt taaataggtt attgctgctt ttaccgttgg tttgacgaag
     3661 ctcagctaag tttttatata aactgccatg attctcatca atagacatct cattatcgtt
     3721 tactaatacg attagattgg attgtaaaac accaccattg ttcaatcctt caaaagctaa
     3781 gccaccactt agggagccgt caccgataaa tgctaccaca tttcctgatt ctttcttcag
     3841 atctctcgct ttagctaaac ctgttgctaa gctgatagat gtagatgtgt gccccactgt
     3901 aaaatagtca tgtttacttt catgttggtt ggaatatccg ctcacttcac ggtaatgtcc
     3961 tttttcaaaa ccattttttc gaccagttaa aagcttatga ccataagact gatgggaaac
     4021 atcccaaacg attttatcct ttggtgaatc aaatacataa tgaaacgcaa tcgttaattc
     4081 tactacacct agattcggac caacgtgccc acctacatcg ctgtcctttt gcaagatttt
     4141 ttggcgcatc tcttctgcca actgtttcat ttcctcaaca gataattttt tgacatctgc
     4201 ggggctattg attgcatcca atacatgtaa tactgtactc ataatacatt ctctcctccg
     4261 tactatcttc ttttaatgaa aatagatttg ttacacttta tctattctac tacttagagt
     4321 tgactctaag tcaatagcat tttaaaaaaa tttcagagaa agcttgttat ataagtagat
     4381 cttcgcacga atcaggactc aaataaaaac cttctacaga caattaaaaa gctttgtaga
     4441 aggtctccta attaaggtct catcaagaaa tatgtttttt taataggtga agttcctgag
     4501 caatatgata caaatggatg tttgcacctt ttaatgagat tggatagatc tttgtctttt
     4561 cagtttctaa tccattttga tagctagcag catcgcacaa aaccactctt tttttcgcac
     4621 atagacattc atcaggcaat tcttctaatg aacgaaataa aataggattt atttcagcga
     4681 tgccttcttc cgcatacaaa tgaaattggg ttcgataaaa aaattgtttg actgaacttt
     4741 ctgaaacaac aaaaatttct atttcctgtc tccccgcttg ttcactgttg atcaaagaaa
     4801 gacttttgac gaattttcga atgaaatttt cattccatct tagttggatt tgatttttct
     4861 ttttccactc ttccaatatt tctacaattt ttccgtacag aagttgttct ttttctgata
     4921 gaagatagat cctttccggt aaaaatactt gtccatctgc ccacaacgta cggacaaatt
     4981 gtaaaaagga ttgcttgaaa ataaaatttt ctgcatcgtc gatttcagcg atactaataa
     5041 tctgtaaaat caattcattc agtgataggt cattgagaac aaaattttta taaacaactt
     5101 cgaaatcttt tcttaacagc tccatgttct cattggtata atttctactg ttgaacaaag
     5161 aaaaaatata atagacatct tcttcttgta agtctagaat tcctttttgc tcttccaatc
     5221 cttgacagac taattgaaat aacggcttcg tttctgcgct tttcttatct tcttctttca
     5281 gtccaagggg aaatgccttt cttccgattg cgatctctat tcctcgtatg ataaaggact
     5341 tttcatcttc cgaatatctt cttttaaaaa aatgagtctc tacataatca atcaactcgt
     5401 tacaagcttc ttctacatac ggatttttat tttcatagcc ttcccaattg acataacgta
     5461 ctaaagctag caagatgttc cgcgagtcaa attctgggat aatgatttct ttatttttca
     5521 aataatcatt ttctttaaaa aaatccaata cgattttttt gattgtatat actttgctca
     5581 aagaaataaa ttcaagatca gccagttctg tactcgtgaa tataccatta aaataataac
     5641 ttaaaaactt taaaaaataa gattgaccgt acagaaactg tgtgagctca gaaagagcaa
     5701 taccatgttt gaatacgagt ttatacttat gcttctctct tacttgttct tttatacaat
     5761 attctagaca ctcggctaga ctttctagat cgtgaaggat cgttggtgta gtcaccctta
     5821 gagaaatagc tgtttgttcg atgtctattt cttggaattc aaataattgt tcacacagat
     5881 acacttttct aaatatattt cgctcgatgt aattttccag cattcctgat acacgcctcc
     5941 agtttttctt atgattttat ttaatcatat ctgtaaatcg tatttttgtt tcctttttat
     6001 ttagaaaagc cctttcaaaa accaataaaa aaagaaactg ggacaaaagt caaacaagct
     6061 cacaatttca gcctcattgt aggtagcttt gagccgtttg gacttttacc ccagcctctt
     6121 tcaagaatgg ttattcaaag atgtcagcgt ctagttcttt gactacatct ttcaacaatt
     6181 ggataccagc tgcgctgtta ccaaatggat ctagtgccgg gctgaagaca cctatgccgt
     6241 aacgatttgg cacagctgcc atcaacccgc cgcctacacc gctttttgca ggtacaccaa
     6301 catggacgga aaactctcct gattcatcat acaaaccagc agttgtcata attgatttga
     6361 cgattgtagc actttcttca gtaatgattt gtttgccatt ccaaggagca attcctttat
     6421 ttgccagcac agcagctaat tttgctatgc ctgtagcggt gacattgagg gagcattgtc
     6481 tgaaataagc atcgagtatc tccggtacgt ctccttcgat catttgatta cctttcatat
     6541 agtaagcaag agagcgattg atatcacctg ttcttgattc agaatgatag atttcttcat
     6601 tcaatgtgat attaggatca tcacagatct ctttcataaa tgacaagatt cgctcaaatt
     6661 tttcatctgc atctttaccg gcaatcaacg aggttgttgc aatcgctcct gcattaacaa
     6721 aaggattcat tggatgtttg cgatgattga tttccatatt catgatagaa ttgaaggcaa
     6781 aaccggttgg ttccgtgttg attgtttgaa agactttttc tattccgttg tctttgattg
     6841 ctaataacaa tacgggaact tttgacatgc tttcgatggc aaagcgcaca tcggcgtctc
     6901 ctccctcaat cagttgtttt gtatccaaat cataaatcgc catacctaat tgtttgggat
     6961 ccacttcagc taatgctgga atgtaatccg ccacttgtcc tgtttgataa tagtggaaat
     7021 tttttccaat caattgttct aattctttta cttccatttg aataaccagc tttcattttt
     7081 tatttttctg catgctggaa tttatgacgg catgcgtaga tcgtaaatgg aataactaag
     7141 acgataagat aagaaacaat cagaatcgtt tcataaatcc catgctgtcc agctgcgata
     7201 ctagcaggtg gaacaaatga gatacaaaaa gcaaaaagtg aagtcaaaag acctaaacaa
     7261 gcaacgatta cttttacaac ttttccgcct ggaacctgat aggctctttc aagactgtca
     7321 tctttaaaaa ccagcttcaa atagcccgcg aaaaacataa agtacaacat caggtagatt
     7381 acaacagtca atgacatcgc tgcaagaaat gaaagattat tccccccgcc gcttaatgta
     7441 aagacggctg cccagattgt tgcaacgatc ccatttgcga tcatcaaatg agcaggaaca
     7501 ccgtgtgtgt tttcttttgc aaaatattca ggaagtaatc cttcttctgc tgctacgtga
     7561 agtgcttttg aaggagaggt tacccaagaa gcaatttctc cgatgacacc taaacaaaca
     7621 agaatcgcaa gaattttcgc aatccatgca agatttggat caatgtgtct taataggaat
     7681 tctaacgatt gaatcacccc tgtattaaga gacaaaccat gttgaggtac tgtagtagcg
     7741 acggtcaatc cgccaaaagt atctaaaatg attgccagaa taaccaatac aaacattgct
     7801 aatggataat cctttttagg gttttccatt tcatttgcat gactggcaga tgtttctacg
     7861 cccatatatg ctaaaataaa ggaaacaaaa acaaccagcg taggcaattt cgaaaaatca
     7921 ggaatcaatg ttttcataga cagtatcgtt tgtagtggag cgcctgaagc gacataatgg
     7981 atccctaagc caaacaagac taatgctggc aacaagatac cagctacaaa accgatccga
     8041 gcaatcaatg aagtgtattt ggttcctttt aactgtgaga gtaaaatagc ccagaaaata
     8101 atcaatagga ttgccaattt cagatattta ttgttttgta tagcaccaat acccgttgca
     8161 taggataatg caccagttaa gaaatacagc atggtcaagt agccaactgt tatctcaaac
     8221 cattggaaaa agattgcggc aaaaccgaat cgttttccca atgtgcgact gacccaagta
     8281 tagacaccgc ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca
     8341 gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc
     8401 gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca
     8461 aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt
     8521 ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct
     8581 tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact
     8641 gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg aatccctaag
     8701 aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga
     8761 atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat
     8821 tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc
     8881 agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca
     8941 acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc
     9001 agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct
     9061 tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt
     9121 cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc
     9181 tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg
     9241 actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc
     9301 cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct
     9361 tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac
     9421 atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg
     9481 aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata
     9541 tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg
     9601 gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa
     9661 aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct
     9721 gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct acgattacga
     9781 gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta
     9841 taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa
     9901 ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt
     9961 tatttttaaa gatatttttt cataaggaat aaagaatact t
//
LOCUS       DAYYGS010000094         9421 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000094 DAYYGS010000000
VERSION     DAYYGS010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.094"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(152..820)
                     /locus_tag="ACHA44_002368"
     CDS             complement(152..820)
                     /locus_tag="ACHA44_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HFM8905620.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            954..1241
                     /locus_tag="ACHA44_002369"
     CDS             954..1241
                     /locus_tag="ACHA44_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905621.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1657..3195
                     /locus_tag="ACHA44_002370"
     CDS             1657..3195
                     /locus_tag="ACHA44_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HFM8905622.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3192..4157
                     /locus_tag="ACHA44_002371"
     CDS             3192..4157
                     /locus_tag="ACHA44_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905623.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4144..5466
                     /locus_tag="ACHA44_002372"
     CDS             4144..5466
                     /locus_tag="ACHA44_002372"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFM8905624.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5529..5633
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5707..6450
                     /locus_tag="ACHA44_002373"
     CDS             5707..6450
                     /locus_tag="ACHA44_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905625.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6465..7457
                     /locus_tag="ACHA44_002374"
     CDS             6465..7457
                     /locus_tag="ACHA44_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905626.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7469..8206
                     /locus_tag="ACHA44_002375"
     CDS             7469..8206
                     /locus_tag="ACHA44_002375"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905627.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8196..8918
                     /locus_tag="ACHA44_002376"
     CDS             8196..8918
                     /locus_tag="ACHA44_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFM8905628.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(9002..>9421)
                     /locus_tag="ACHA44_002377"
     CDS             complement(9002..>9421)
                     /locus_tag="ACHA44_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFM8905629.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3099 a         1614 c         1978 g         2730 t
ORIGIN      
        1 aaaataatgg caaaataaaa aaacaaacta ttttcgcaga tcgtttgcaa aaatagttta
       61 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
      121 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      181 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      241 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      301 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      361 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      421 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      481 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      541 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      601 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      661 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      721 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      781 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      841 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      901 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      961 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
     1021 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1081 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1141 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1201 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1261 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1321 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1381 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1441 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1501 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1561 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1621 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1681 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1741 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1801 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1861 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1921 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1981 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     2041 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2101 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2161 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2221 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2281 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2341 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2401 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2461 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2521 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2581 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2641 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2701 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2761 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2821 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2881 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2941 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     3001 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3061 aaattaagcg gaaaaataac agaggatcaa atgattgata tgaattacca agtcaatgtc
     3121 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3181 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3241 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3301 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3361 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3421 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3481 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3541 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3601 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3661 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3721 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3781 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3841 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3901 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3961 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     4021 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4081 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4141 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4201 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4261 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4321 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4381 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4441 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4501 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4561 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4621 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4681 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4741 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4801 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4861 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4921 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4981 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     5041 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5101 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5161 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5221 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5281 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5341 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5401 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5461 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5521 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5581 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5641 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5701 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5761 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5821 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5881 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5941 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     6001 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6061 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6121 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6181 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6241 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6301 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6361 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6421 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6481 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6541 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6601 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6661 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6721 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6781 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6841 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6901 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6961 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     7021 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7081 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7141 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7201 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7261 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7321 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7381 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7441 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7501 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7561 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7621 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7681 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7741 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7801 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7861 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7921 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7981 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     8041 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8101 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8161 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8221 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8281 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8341 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8401 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8461 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8521 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8581 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8641 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8701 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8761 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8821 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8881 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8941 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     9001 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9061 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9121 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9181 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9241 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9301 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9361 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9421 a
//
LOCUS       DAYYGS010000095         8817 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000095 DAYYGS010000000
VERSION     DAYYGS010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.095"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(137..427)
                     /locus_tag="ACHA44_002378"
     CDS             complement(137..427)
                     /locus_tag="ACHA44_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905630.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(545..805)
                     /locus_tag="ACHA44_002379"
     CDS             complement(545..805)
                     /locus_tag="ACHA44_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905631.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(942..1355)
                     /locus_tag="ACHA44_002380"
     CDS             complement(942..1355)
                     /locus_tag="ACHA44_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HFM8905632.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1811..2296)
                     /locus_tag="ACHA44_002381"
     CDS             complement(1811..2296)
                     /locus_tag="ACHA44_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905633.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2435..2650)
                     /locus_tag="ACHA44_002382"
     CDS             complement(2435..2650)
                     /locus_tag="ACHA44_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HFM8905634.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2874..3674
                     /locus_tag="ACHA44_002383"
     CDS             2874..3674
                     /locus_tag="ACHA44_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8905635.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3693..5561
                     /locus_tag="ACHA44_002384"
     CDS             3693..5561
                     /locus_tag="ACHA44_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HFM8905636.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5554..6045
                     /locus_tag="ACHA44_002385"
     CDS             5554..6045
                     /locus_tag="ACHA44_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFM8905637.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6033..6587
                     /locus_tag="ACHA44_002386"
     CDS             6033..6587
                     /locus_tag="ACHA44_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFM8905638.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6605..7600
                     /locus_tag="ACHA44_002387"
     CDS             6605..7600
                     /locus_tag="ACHA44_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFM8905639.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7742..>8817)
                     /locus_tag="ACHA44_002388"
     CDS             complement(7742..>8817)
                     /locus_tag="ACHA44_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8905640.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3046 a         1425 c         1683 g         2663 t
ORIGIN      
        1 ttgttactat gaataacata ataagaaagt aaagtggatt gatttgagat ctttttatac
       61 ttcaaaaaag ataacaccca actttcaaaa atatgttttg gaagttggga caaacgagtt
      121 aatacatttt aatacattat tttttattca aaaaaaatag cacaatggca aagatcgtaa
      181 gagagatcat aagccccaat gaatgactaa aaagaaaagc agaatcacat gccatatcat
      241 cccatatttc ttcaagaaaa taaataagcg ggaaaaaggg aaggaatagt agaacatcga
      301 tccagcacca tttttttctt cttttgaaat aacataaaac aataaacatg aacgtgcagg
      361 gaagtaaaaa caaagcttta ctcataacta acaaaatacc agctcctttt atttttttct
      421 cccccacatt caaagagtaa caatggtatg tacttaataa tagaaaaatg gatcaacttg
      481 tatgaataac agctgaaaag atctgcacca aataaaaaac ttccagccaa aactgactgg
      541 aagtttattt ttttctgaaa tattctaaca caaatatgaa aaagatggcc gaagctaata
      601 agccgatttc gtagcaaaac aatgagatac cagagtcgta ataataaaat cctctaatct
      661 tatggataaa atagatcatc ggacaagcag ataagagtaa gtatttgtta ggccttttga
      721 ataagtaaaa gaaagcactg gtcattatga taaatggtat ccaaaacaaa agtttactta
      781 agacaatcaa atgatcaact cccatatttt ttaccaagag agatcataac atctttatcg
      841 taaaaacaaa atattttttt gctcattttg aaaatttaaa catactcgtt cttatccaaa
      901 tatataaaaa tcgcccctca ttcatcgaga ggcgataagt tctaatttaa ctcaattttt
      961 tttgtttctt ttggctgata agctgtttta ggtaatacca atttcagcac accgttttta
     1021 aaggttccag tgattttttc ttcatcaaca ttaggaagat agaatgaacg tttgtaagag
     1081 accgaatgtc tttctttccg tacatagttg ccatcttctt tatcttctgt atgggactca
     1141 tgattggcgg agatcgtcaa agtattatct gaatattcaa ctgtcgtgtc ttctttgtca
     1201 cagccaggca tatcagcagt tagtttgtat tctttttcat tttcgactaa atctactttt
     1261 ggataacttg aaacacctaa aaaatcattg aatgctggac taaacacgtc attgaaatca
     1321 gggaacatgt cgcgaatgct aggtacattt gccatttttg atcatccttt caataagtat
     1381 tttttattta tccctattta cgtaataact ctattttgaa aaaaatgcaa gtataatggt
     1441 tagctttgtg aatatttcat ctaatacgat acaaaggcta tctttctaat ttgaaaaagg
     1501 gatcattaaa ataataagaa gaaaatatgt caaaaggtaa aaaagtaaat tcttctatcc
     1561 aaatatagaa aaattgaatc tagaaaaccg cccgacaata aatttcatta actcagttga
     1621 ccgaaaatga agtagatacc aaaaatttat caagagagca agtagagaag tgagtatatt
     1681 cataagagaa atagctgtaa cgaattatta ggttaatgca aatgcttatt tatactccgt
     1741 ataactttct cagaaaaaat gataccaata atgacttgct agtactgtaa atccgtataa
     1801 tatcatctaa tcaaaatggt gttccatttg catctgtcat cgtaatcgat ccttcgggtg
     1861 acactcgata aaaaccaacg cttccagatc ctccttggga acggatttgt ttgctggaag
     1921 ctgtgaacaa ataatctcga tcaatttttt gataaaagct aataacaata tcgtcacttt
     1981 gtaattcgga acggtgggca ataagaaata gaagtgcttt gtcagagttt tctaatggaa
     2041 gagaaggtac agtgctttct tgcatttcag atgttgaaga aatgctaaac gactgaatac
     2101 ttgattggct atgttgcgaa tctacattac cagtagaagt attagtaatg gaagtagtag
     2161 aagtactttt agttgaacca acctcagttg ttgaaatggc tgctgaagat tctttaacag
     2221 aattctttgt tttatatcca caggcagaaa aaagtattac agttcccaac aagcctgcgt
     2281 aaatcccttt atgcatgata gctcctccta aaatagatta tctttattca gtatatcact
     2341 taaacaaaga aaagcagatg gtttaaaatg aaaaaacctt cccaataaag ggaaggttca
     2401 tgaagataat aactttttta ataattatag ctttctaata tctttcatca acttttctag
     2461 taatcattgg gaaaaatttc aagcagtaga tcgcacaaat acctacgacg atataaatga
     2521 tcttcgcaaa aatagttgtt gaaccaccag caatcatagc tactaaatca agttcgaaca
     2581 agcctaccaa cagccagttc aaccctccca caatcaataa agttaatgca atagcatcta
     2641 aagttttcat tagaattctc ctttcttaaa tgataaaaca attatatatc aaaaagagaa
     2701 acattgctta tataacgctc aggaatttta gcaatctatg caaaaagatg aaaatttttt
     2761 ttgctcttcc attagtacac attttttagc tgaagctgta aatttcatgt cttaattttt
     2821 tttagtgagc ttttatactg gatttgtaag aaaaaagaag gagaggatca catatgtcgg
     2881 attggttgga tattgcagga aaagtagtaa ttgtaactgg aggttcctca ggaattggaa
     2941 gaagtatcgt cgaaaatttg ttgaagcaga atgcacaagt agcgaatttt gatgtaactg
     3001 aatgccgtat acagcatgaa aacctactat ctttaaaagt cgatgtgtct tctaaaacgg
     3061 acatagaaga agggatatat aaagtaatga aacatttcaa gacgatcgat ggtctagtca
     3121 ataatgcagg aataaatatt ccttctcttc taatagatag aaatcatcca aaaagcaagt
     3181 atgaattatc agaacaagtt tttgataaga tgatcgctgt caatcaaaaa agtgtatatt
     3241 tgatgagcca agcagttgga agaatcttgg tgcaaaaggg atcaggagtg atcgtgaatc
     3301 tttcttctga atcaggattg gaaggatcag aaggacagag ctgttatgct gctacaaaag
     3361 cagcgatgaa cagttttact cgttcttggg caaaagagtt aggaaagagg aatgttcgtg
     3421 tggtaggtgt tgcaccaggg atactggaag aaaccggttt gagaacacaa gaatatgaag
     3481 aagctctttc ttatacgaga ggtataagcg tcgaacagct gagaaacggt tacagtcgaa
     3541 caagtacgat ccctctagga aggagtggta agctgcaaga agtggcggat ctagtctgct
     3601 attatctttc agaaagatca agttatatca ctggagtcac gactaatatt tcaggaggta
     3661 aaacaagagg ataaaggata ggggggacgc acgtgacgct agtaaacaga tggtaccaat
     3721 tattgaaatt actagtcact cacaagaaag tttcaatcca ggaagtgcaa aaagaaatca
     3781 aaaccagtaa tcaaaccata aaaaaagata ttggggagtt gaatgaagaa attgccggta
     3841 ttgcaaaaat catccaaaaa aacaaacatt ttgaacttga aatttatgat tttgattttt
     3901 ttgatgagat catgcaagga aaattgaaaa cagccagtga tttcaattcg gcatccaaaa
     3961 gaatcgctta tttactaaat aggctgatcg aaacagagga tttcttacgt atggatgatt
     4021 tgtctgaaga agtcggtgtc agcagaggaa cagtggtcaa ggatctaaag acattaaaag
     4081 agatgatcaa tgattttgat gtaaagatta caggcacacc aaataaagga ctaagaataa
     4141 taggagatga gctagaatta cgactacttt tattttattt tgtttctccc tacttttcag
     4201 agacgaaaac agaacagttt ctagaagaaa aacaagcaat caaacatttt cagaagaaga
     4261 cgaatgctag taaacaagaa atcacccttt taacgaaagt tatcggtatt tctttgaata
     4321 gaatctctag taactatctg ttgaagaagc ccatcaatcg ttacagtggg tgttttgaat
     4381 atatccaaga attcaatgaa ctgatcaatc agttggaaga aatctatgaa ctaactttga
     4441 gccaatatga aaaagatttt atcagttttc ctttgaacat catcaacaca agtataacga
     4501 tgataaggaa agctaactct gatttgcgct tatatttcga ccccatgatg atgcgtattc
     4561 gacgattaga tctcattgat ctggatgagt ccttcttata tcaagagatg aaacagcacc
     4621 tctctttatt gataaatcgg gtaatttttc gttatcgatt gactgattta ttttatggtg
     4681 agatagaaaa aaagtatcct ttttcttacc aaatcgctag cgaatgcata caagcattag
     4741 aagctatctt gcaacataag atctctaaag tagagatcag ctacctaacg ttgtatttgg
     4801 agttagcatt tcgatatcaa aaagagcatt tttcctctaa aaaagtagcc attgttcata
     4861 cgacaggaaa aggaacagca ttaatgatcc aaagacaaat caaaaggata ttggggaggg
     4921 atatacaaat aacacatgtt tcagaagaac aatatgaaaa agtagatcta aacaaatatt
     4981 tcgctgtttt tacaacgatt ccgttaaaaa atgtaaatga agagacaccg gtgatccatt
     5041 tgactaatct ttttgatgac tcttggttaa gggcacaatg gcaaaaagca aaaaaaaagc
     5101 gattagccgg gtttcattct actgtttttt tctatcaaat atggaaagaa aaaaagacgt
     5161 atcaagaata tttaaacaaa ttagtcgctc ctttgatcag aggctattat gtggatgaag
     5221 aatttgaaaa aaggatcttg aaaagagaat atcagcaaag cacatttgtt aacaaagaat
     5281 ttgcttttcc tcacacaacc aaccagctat ctcaacacat tgttttatca gtagggctaa
     5341 taactgatca aggtttagca gaaacaggag gaaatggagt tattttttta tttgccatac
     5401 ctgaagaaat gacggctgaa gttgagaatg aattatttga gctatacgat atcctatttg
     5461 aactcataaa aatgggaata gatgagttta ttgaattagc accgacacaa gaatcatttt
     5521 taaaactaat caaaagtaaa ggagagagac ttcatgaatg atatgtatct attcatagtt
     5581 ttcgcagctt cagcttatgt gctgcaagca gttatcagtt ggaagcaact aaagcatttc
     5641 aatcaaacat atagcgaact gcgaagaaaa ggaaaagtag cgattggtaa acgacctggg
     5701 aaaatccgtt caggcacgat tgttatgcta gctatagacg atcatgatca agttttagaa
     5761 gtcagaaaaa tgcaaggggt tacatctttg gcaaaatttc attcattacc tgtctatgcg
     5821 ggtgaagata tccattacat cgatcgttac catccctcag ttcgagcgga agatcgtttg
     5881 acacaagcag ctttagaaaa tgccagagaa gtatacttac gaatagaggc aggaaatgaa
     5941 aaaagtgaac aaccttcatt gccttttatt gctatgctca ataattttcg gatatggaaa
     6001 agtacgatca aagaaaaaag gagtgacaaa cgatggatat cataacaaaa tttgctgagg
     6061 gatttatgag gctgttccaa acaggtgcag aaacattcat aagctggatg acaggaatcg
     6121 taccagttgt cttgatgctg atggttgcaa tgaatacgct gattgctttt ttaggtgaag
     6181 accgagtaac gaaagtagcc aaattatcag cgaaaaatcc gcttactcgt taccttgtgt
     6241 taccgttctt atctgctttt atgttgggaa atcctatgtc atttacaatg gctcgtttcc
     6301 ttccagaata ctacaaacca agctattatg ctgctcaagc tcaattttgt catacgagca
     6361 atggtgtatt cccccatatt aatcctggag aattgtttgt atggatgggg attgctcaag
     6421 ggatcgaaac cttagggcta aattcgatgg agctggctat ccgatacttg cttgtgggac
     6481 tgctgatgaa ctttattggt ggttggataa ctgattttac tactggattt gtatgcagac
     6541 agcaaggaat cgttcttagc aaaaaagtag agctttcagt tgattagaga aaggagagtt
     6601 ttaaatgtat ggtgatatta agatcgtaag aggaaacggc gggtatggag gtcctttgat
     6661 tattaggccg acaaaagata aacacaagtt catctatgtc acaggtggag gtgagaggcc
     6721 aatgatcgta gataaaatag aagaactgac aggtatggag tctatcaatg ggttcaaaac
     6781 atcaatacct gatagtgaaa tcgcgcttgc aatcgttgac tgtggaggga ctcttagatg
     6841 tggaatttat cctaaaaaag ggataccaac catcaatatc gtttccacag gaaaaagtgg
     6901 cccattagca caatatatca ctgaagatat ttatgtttca gcagtagggt tagaacaaat
     6961 acagccacta atggaaactg atgataacaa agaaacagta aatactgcat ctccagataa
     7021 aaagtacaaa tatgatactt ctaaaaagat aaccgagcaa aaagcagagc agcaaaactg
     7081 gatagcaaaa attggaatgg gcgcgggaaa agtagttgct acattcaatc aatcagcaag
     7141 agaagcagta gatacgatga tccggacagt gatccctttt atggctttcg tttccctttt
     7201 gattggtgtg atacaaggtt ctgggatcgg taatgtattt gctaacatta tggcaccttt
     7261 agcaggaaat gcagttggat taacaatcat tggctttatt tgttcactgc catttttatc
     7321 tcctttgctt ggtccagggg cagttattag ccaagtgatt ggaacattga tcggtgtaga
     7381 gattgggaaa ggaaatattc ctccccaatt agcattaccg gcactatttg ctatcaatac
     7441 tcaaaatgca tgtgatttta ttccagtcgg tctaggtttg gaagaagcaa aagctgagac
     7501 agtagaagta ggtgtaccat cagttcttta ttcaagattt cttaacggca tcccacgagt
     7561 tatcattgct tgggtagcaa gttttggatt atatagctga gtttagtcta atggtgataa
     7621 gtcaagatga aatttaagat tttctttatt gagtaatatg ttttgaagcg cacgaaacta
     7681 gacccgatca aaatcaaatt tttttcgggt tgattcaagg atttgcgtca ataacggatt
     7741 gttatttctc tatcacactc cttggtggcg tatagagttg gtgtttacgt agtagcgtat
     7801 ccaccagacg cgtaaatttt cttgcggtta ggacgagtgc acgtttgtgt tgatgctttg
     7861 gaacctcctt atatttactt tgataaaaag ctttgtattc aggaagataa ttttttacag
     7921 agttggcagc ttcaactaag tagtatctaa aatatcgatt tccttgtttt gtcagaggtg
     7981 tattttgcga ctgatagttt ccagattggt tcactttcca acttaatcca gcgtattttg
     8041 ccaattttgt ctgatcttca aatctttcga tttgaccaat ttcagcgatt aaaccggcag
     8101 catagacttt accaacgcct ggaatactgg ttaagcattg atattctgga atgacaacaa
     8161 ttacttgttc gatggcttta tctaattctt tgatattttt ttcaagtgct cgtatttctc
     8221 gtacgagaac acttaaaaca acattaattg attcttgtgc aagagcacct aggcggtaac
     8281 tcatggtaat tgcacgttga attgctttag cgattttttc tggttgcttg aaacggcctt
     8341 ttcctttttc ttgaagtaaa tcacagaaag cttctaaagg aagctcagca agttcatcca
     8401 atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc gtcgattcgt
     8461 ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga aaatgttgct
     8521 ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc tgtaaggcca
     8581 tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag aaatcagcga
     8641 ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta aattgtttca
     8701 cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc tcatcttcat
     8761 gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca atcacaa
//
LOCUS       DAYYGS010000096         8695 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000096 DAYYGS010000000
VERSION     DAYYGS010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.096"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     regulatory      74..153
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SMK box riboswitch (SAM-III) riboswitch; SAM
                     riboswitches modulate the expression of downstream genes
                     in response to levels of S-adenosylmethionine; SMK box
                     riboswitch shares no known similarity to SAM-I and SAM-II;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            151..1344
                     /gene="metK"
                     /locus_tag="ACHA44_002389"
     CDS             151..1344
                     /gene="metK"
                     /locus_tag="ACHA44_002389"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFM8905641.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKEDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1474..2946
                     /locus_tag="ACHA44_002390"
     CDS             1474..2946
                     /locus_tag="ACHA44_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFM8905642.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3029..3817
                     /locus_tag="ACHA44_002391"
     CDS             3029..3817
                     /locus_tag="ACHA44_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8905643.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(3884..4552)
                     /locus_tag="ACHA44_002392"
     CDS             complement(3884..4552)
                     /locus_tag="ACHA44_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HFM8905644.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            4667..6379
                     /locus_tag="ACHA44_002393"
     CDS             4667..6379
                     /locus_tag="ACHA44_002393"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905645.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6382..8154
                     /locus_tag="ACHA44_002394"
     CDS             6382..8154
                     /locus_tag="ACHA44_002394"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905646.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(8236..>8695)
                     /locus_tag="ACHA44_002395"
     CDS             complement(8236..>8695)
                     /locus_tag="ACHA44_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905647.1"
                     /translation="ESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEII
                     DQLIEENKLIKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSH
                     ISLLTELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT         2661 a         1437 c         1876 g         2721 t
ORIGIN      
        1 aaaatatttt tatatttttt aagcaaaaaa atgaaaaaat acgagcagaa aactgtttta
       61 tctgctatac ttggttcgtt gcaagttccc gaaaggatcc gcaggacgga gatgccttgt
      121 aaccgaattt aactaggggg aatcattata atggtagaaa gacacttatt tacatcagaa
      181 tctgtttctg aaggacatcc agataaaatt gctgaccaaa tcagtgatgc aatcttggat
      241 gcaattttaa aagaagatcc aacagcacga gtagcgtgtg aaacgtctgt aacaactggt
      301 cttgttttag tatttggaga aatatctacg acggcatatg tagatattca aaaagtcgta
      361 cgggaaacaa taaaagaaat tggttataca cgtgcaaaat tcggatttga tggagataca
      421 gcagctgtat tggttgcgat tgatgaacaa tctcctgata ttgctcaagg agttgatgaa
      481 gcacttgaga tccgggatga agataaaaaa gacgtattag atgagattgg tgctggtgac
      541 caaggtttaa tgtttgggtt tgctgtagat gaaacaccag aattgatgcc tttaccaatc
      601 gctttgagtc atcgtctggt acgacgcttg gcagatttgc gtaaatcaaa tgaattaacg
      661 tatttacgtc cagatgcaaa atctcaagta acggttgaat atgatgatca aggacaaccg
      721 gaacgcgtag atacaatcgt tatttcaaca cagcatgatg atgcagtaga taatgaaaca
      781 attcgtcatg atgtcatcga aaaagtagtg aaggaagtta ttccagctga attattagat
      841 gatcaaacga aatattatat caatccgact ggccgatttg tcattggtgg tcctcaaggg
      901 gatgccggat taacaggaag aaaaatcatt gttgatacgt atggcggtta tgctcgtcat
      961 ggtggcggtg ctttttctgg taaagatgcg acaaaagttg accgttctgc cagctatgct
     1021 gcacgttata ttgctaaaaa cattgttgca gcagggcttg ctcgcaaagc agaagtacag
     1081 ctagcttatg cgattggtgt tgctcaacct gtttcgatct caatcaatac tttcggtact
     1141 ggaacggttc cggaagaaga actgattgct gcagtgagag aaaacttcga tcttcggcca
     1201 gcaggaatca tcgagatgct tgatttgcgt cgtccgattt acaaacaaac agctgcttac
     1261 ggtcattttg gtcgtacaga tgtagatttg ccttgggaac atacggacaa agtagatgcg
     1321 ttgaaagcaa gtttagcaaa ataagttata ttcagctgga acgatcgcca agtggtggtt
     1381 gttccagctt tttttatggc agttaacctc ttgaaaaaga tagtaattat ttatctattt
     1441 ttagatcctg tcataatgaa agggaggaat aagatgaaaa aagaaacaaa tgttactttg
     1501 gtcacagtga gtattttcat tgcaacgttc atgacggcta ttgaaggtac aatcgtaaca
     1561 accgctatgc caacgattgt cggttcatta catggaatgg aaatcatgaa ctgggtattt
     1621 tcgatttatt tgctgacaaa cgctatgctt actccgattt acggaaaact tgcggataaa
     1681 gtgggacgga aaccaatctt tatttttggg acattattgt ttattatcgg ttcgttattt
     1741 tgcggtttat ctcaaagcat gattgggttg attgctgctc gtgcgattca agggatgggt
     1801 gctggggcaa tgatgccagt agctttgacg atcatcggcg atctttattc tattgaaaag
     1861 cgagcaaaag ttttaggttt gaatagttca gcctggggga ttgccagtgt ttttggaccg
     1921 cttgcgggag gattcatcgt tgaaacgatc agctggcact ggatcttttt tatcaatgtg
     1981 ccgatcggaa ttctattaat tattttgatt tggctatatt taaatgaacc aaaaatcata
     2041 catgagtcta aaccaatgga tatttttggt agttttagtt tgatggccat gcttgtctca
     2101 ttgctgttag ggttccaatt attaggagac gaaggaatga gtccgcttgt ttctgctttg
     2161 ctgctaggta gtatcgtaat gttaggtatt tttgtttttg ccgaaaaaag agctaaagat
     2221 cctgtcatca tgatgcattt attcaaaaat ccactatttg ttgtggtcaa cttggctgca
     2281 gcattggcta gtggtttttt gatgggaatc gatgtttata tccctatgtg gatgcaaggt
     2341 gtattagggc tgaatgcagc aattggtggg ctggtattag caccattatc actagtctgg
     2401 atggtcggtt ctttccttgc aagcaagtgg atgactcaaa tgaaaactca ttgggtcttg
     2461 agaattggac tactaatcgc agctattggg ggcgcttggt tgtatcttat gcctttcaga
     2521 agtaactatt tatggttttt tgctatatca acggtcatcg gtataggctt aggtgtgacc
     2581 atgactacct cgactgtcag tgcacaaaat agtgtatctc gtgaccagct tggtgtagca
     2641 acttctttta atacgttagt tcgaacaatt ggtcaaacag tcatggtttc tatttttggt
     2701 ttattgataa atggggtaac taaacgagaa cttgcagcag caaatttaac aaatgatgca
     2761 gatatcatga ataaactggt gaatccacaa acggctaaat atattgatgc ggatattttg
     2821 ttaccattaa gaaaaattct ttatgatggg ttacattccg tttatctagt tggcttgtta
     2881 ctcgtgatca ttgctttggt cttaacgctt ttcgttcatg aaaaaagtgg taaaataaaa
     2941 cagtagtttc ggttaagata tgtacgtagc cgaatcattc aaatttaaag ttattcaaaa
     3001 ataacttctt actaaggaag gcgaaaatat ggcatttatt caagcaaata tttattcgaa
     3061 tgtgttagaa atggaagtca atctaaatgt gatactaccg caaacaacaa aaaaggtgat
     3121 tggcaccagt tcagagcaag agacaacgga tatacctgtt ttatatttac ttcatggaat
     3181 gggtggcaat catagtgtat gggaaagacg tacgtcgatt gaacgttatg tagcaaacta
     3241 cggattagct gtcatcatgc catctactga tcttggctgg tacacagata cagtttatga
     3301 catgaagtat tggacattca ttgcagaaga gctacctgag atttgtcatg aattattccc
     3361 gcaattaaca agaaaaagag aaaaaacata tgccgctgga ttatcgatgg gcggatatgg
     3421 cgcgttaaaa ctaggccttg caaaatcaga aaatttcgca gcagtaatct ctctttcagg
     3481 cgctgtttct gtaggaaatc gaatggatga tcttttgatg gttcgtaaag catctttttg
     3541 ggaagggatc tttggaccat tggatcaaat cgaaggttca aaaaatgatc cgcaatactt
     3601 attgaaagaa ctagtaaaaa gcggaaaacc agttccaaga ttttatatgg cttgtgggga
     3661 atctgatttt ctttatcagg caaatcagga aatggtatct gctatgcagg aacaaggctt
     3721 ggcggtcacc tttgaacaag gacctggcga tcataactgg atcttctggg accaatggat
     3781 tcaacgtgca ttggcgtggc taatggaaga aaaataaaaa aagagtctta aaaagatagc
     3841 tcctatagag atcacaaaca tagtgatctc tataggagct tttttactgc tcgttttctc
     3901 ttcttttatt ttgttggcat aagccttcaa tataggtttg tgcactcgtt agaaagagtg
     3961 ctttttcatt caattctgca tgggactgca tactttcttt aaatccctct aattgatttt
     4021 caactacata attacgcata aatagaactg tttctttcaa aaattcattt ccgtccataa
     4081 ttttttgtcg aagctgctgt tcatgctgct gatccattaa ctgtccaatc aataaattgt
     4141 ggagagaggc aatggcctga tgactttggg tgactgaaaa tccttcacga acaagtaatt
     4201 ggatcatttg ctccaaatgt tctaatcgac tcggaaaagc gggcacagta ttgatttcta
     4261 attctgcccc acaagggaaa cgtgtataaa ggtcatagta attttccata tactgcagca
     4321 actgttcatt ccaaggcaaa gttggatctg gaagcgctaa ttcttcttcc attgtttcag
     4381 ccattatttg caagagctgt tgtttgtttt tgaaatacca atataaagca ggtgcttgaa
     4441 catgaattct gtttgcgatg tttcgcatgg aaagctgcga aagagcttgt gtttcagcca
     4501 ggatatcaaa ggcggcctga atgatgattg cttgagaaag ctttatttcc attttatcaa
     4561 cctcgtgtat tgcattatct ttttaaacat ggtaaattat tagtcggtct aattttacag
     4621 tgttaaatta gtcctgtcaa tttaaacaaa ggggtgtaat caacgaatga aattaatctt
     4681 acgacatgcg aaaaaatata aagtagctgt ttttatttcc ttgctgtccg tagcagtcat
     4741 ggttgcagca gcattatggc aacctaagtt actacagcaa gtattggaag ctattattac
     4801 agaagataat gatgaaatga aaacaattgg tatttacctg atcagtctgg ctttgctagg
     4861 attagccgca ggtgtaacga acacgatttt ctctgcaaaa gtagctcaag gcgtaagtgc
     4921 agatatccga gaagaagcat ttcgaaagat ccagacattt tcatttggaa atatcgaaca
     4981 gttctctgct ggaaatcttg ttgttcgtct aacaaatgat atcacgcaaa tccaaaattt
     5041 agtgatgatt tctttgcaat cactttttcg aattccattc ttatttattg gtgccttcat
     5101 tttagctatg atcacgatgc cgcaattatg gtggatcatc gtagcgctta tcgttacggt
     5161 atttctgatt acggcacttt catttacaag gatgggcaag cacttcatga tcattcaaaa
     5221 actgattgat cgtgtgaata gcattgcaaa agaaaactta ctagggattc gtgtagtgaa
     5281 atcttttgta caagaagaaa accaattgaa gtcattcagc aaagtaagtg aggacttaac
     5341 aaaacacaac atcatagtcg gtacattatt ttctgtgatg atcccgtcct tcatgctggc
     5401 tgccaatttg gctgtggttg gggcgatctt ttttgtgagc gatcttgtta aagatgatcc
     5461 tacgttaatt ggtggaattg cttcattcat gaactacttg atgcagatta tgatggcaat
     5521 cattattggc ggaatgatga tgatgatgac ttcaagagca gccgtatcgc tgaagcggat
     5581 tgcggaaatt ttagatgctg aaccagatct tacttattta gacgttccag aacaagagtt
     5641 gacgggttct gtcacttttg accatgtttc ttttcgttat cctggtgagg atacagatac
     5701 gcttaaagat atcagctttt ctatcaaacc tggagaaatg gttggtatcg tcggggcgac
     5761 tggtgccggg aagtcaacac tagcacaatt gatccctcgt ctatttgatc cgacagaagg
     5821 caaagttgaa gtaggtggtg tagatcttcg tcaagtaaat gaaaagagtt taagacaaac
     5881 ggtttccttt gttttgcaaa aagcaatttt attctcagga acgattgctc aaaacttgcg
     5941 ccaagggaaa aagaatgcaa cagaaaaaga gatggaacat gcttcttcta ttgctcaagc
     6001 aaaagaattc atcgaaaagt tatctgatcg ttacgaagca ccaatagaag aacgtagcag
     6061 taacttttct ggtggtcaaa agcaacgttt atccattact cgtggcgtta tcggtaatcc
     6121 aaagatcttg atcctagatg acagtacgag tgcactggat gctcgctctg aaaagctggt
     6181 ccgagaagca ctcgatcgag atttgaaggg aacaacgacg attgtcatcg cacaaaaaat
     6241 tgcttctgtt gtcaaagcag accgcatctt agtattggat gaaggcagat tagtcggcga
     6301 aggaacccat gaggaattgg tagctggaaa ccgtgtctat caggaaatct ttgaaactca
     6361 aaaaggaacg gaggagtaag tatgactgat ctaataaaag caagtaaatt tttctatcac
     6421 tatttgaagc ggtacaaact ctcctttttc tttatcttta tcacagtgat cattgctacc
     6481 tatctacaag taaaggcacc tcaatacatc ggggaagctt ttcaagaatt agctaattac
     6541 gtaggaggct tgatgcaagg cgtagacgat aaaagcaaat ttgtttctgt aatatggaaa
     6601 ctcttgctgt tttatgtact agctagtgca gccaacttta tctatagtat tttgttcaca
     6661 caagtagtag ggaagtcaac gaaccgaatg cgtatcggtc tattcaacaa attggaaaaa
     6721 ttgacgatcc gtttctttga ttctcatcaa gacggtgaaa ttttaagtcg ttttaccagt
     6781 gacttagata atattcaaaa tagtttgaac caagctttgc ttcaagtcat cacgaatgct
     6841 gttttacttg tcgggatctt gatcatgatg ttccgccaaa atgtagagtt agcttgggct
     6901 acaattgctt ccactccagt agccgttttg atagctgtct tgattatccg caaagcacgg
     6961 aaatatgtgg atcttcagca agatgaagtg gggaaactaa atggttatat ggatgaaaaa
     7021 atcagcggac aacgtgtcat catcacaaat ggcttacaag aagaaacgat tgaaggattc
     7081 ttagagcata atcaaagtgt tcgcgaagca accttcaaag gacaagctta ttctggtcta
     7141 ctatttccga tgatgcaagg aatgtcactg gtcaatacag cgatagttat ctttttcggc
     7201 ggctggttag cattaaatgg tgatttggaa cgttcggtgg cattaggctt agttgtgaca
     7261 ttcgtccagt attcacaaca atattaccaa ccactgatgc agatttcatc tggatacagc
     7321 atgatccaat tagcgattac aggggcaaga cgattgaatg aaatatttga tgaaaaagat
     7381 gaagtcaaac cagaaaactg taaagcattt gatggtgttg aaaaaggtct tgcgttgcag
     7441 cacgtagatt ttggttattc tccagaaaaa ctagtcttga aagatgtctc gatcaatgtg
     7501 gacaaaggag aaatggttgc tcttgttggt ccaacaggat cggggaaaac gacgatcatg
     7561 aatctgctaa accgttttta cgatgtcaat gatggttcag tagtcatcga tggaatagat
     7621 atccgagatt atgatcttga tgcattacgt agacatgtag gaatcgtttt gcaagaatct
     7681 gtattgttct ccggaacgat caaagacaat atcacattcg gcaaaccgga cgcttcagat
     7741 gaagaagtca tcaatgcagc aaaacaagcg aatattcatg acttcatcat gacgttagaa
     7801 aaaggttatg atacagagat atccgaagaa aataacttat tcagtacagg tcaaaagcag
     7861 ttgatcagta ttgctcgaac gatcatcaca aatccatctt tattgatttt ggatgaagca
     7921 acaagtaatg tagacacggt aacagaagca aggatccaaa aagcaatgga tgaagcaatc
     7981 aaaggaagaa ccagctttgt gattgctcac cggctgaaaa caatcttgaa tgcagataga
     8041 atcgttgtct tgcgtgatgg ggaagtcatt gaagaaggca gtcatcacga gttattagca
     8101 gaaaatggat tttatgcgga actttatcat aatcaatttg tatttgaaag ttaaattaaa
     8161 agaacctcgt tataacgggg ttctttttat atattttatg ttgtttgttt ttataagggg
     8221 cagaaaaggg gcaatttaaa tttctatttt tcctagtttt tctactagtt gattttgcat
     8281 atttttagtt gtatgagtat aaatctttaa tgttgttttt gaatcttcat gtcctacacg
     8341 atccataatg gcttttaatg ggattcctaa ttctgtaagt agagaaatgt gggaatgcct
     8401 taacatgtga gaagaaactt ttttatctag gtttaattct ttggatactg ctcttaaaga
     8461 ttgattatat gcttgaatag atatcgggtt accattttta gagatgaaaa tgtatttatc
     8521 tttattattc ttaattaatt tgttttcttc aattaattga tctatgattt ctttagcatt
     8581 acttgataat tcaatggttc tataagaacc tgtattttta gttgttgttt taatttttaa
     8641 ctcgtaatca taagtaccag aaaccgtaat tttattacca tcataatcag attca
//
LOCUS       DAYYGS010000097         8671 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000097 DAYYGS010000000
VERSION     DAYYGS010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.097"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(262..696)
                     /locus_tag="ACHA44_002396"
     CDS             complement(262..696)
                     /locus_tag="ACHA44_002396"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905648.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(928..1638)
                     /locus_tag="ACHA44_002397"
     CDS             complement(928..1638)
                     /locus_tag="ACHA44_002397"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFM8905649.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(1628..2488)
                     /locus_tag="ACHA44_002398"
     CDS             complement(1628..2488)
                     /locus_tag="ACHA44_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFM8905650.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(2493..3131)
                     /locus_tag="ACHA44_002399"
     CDS             complement(2493..3131)
                     /locus_tag="ACHA44_002399"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFM8905651.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3146..3445)
                     /locus_tag="ACHA44_002400"
     CDS             complement(3146..3445)
                     /locus_tag="ACHA44_002400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905652.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(3488..4972)
                     /locus_tag="ACHA44_002401"
     CDS             complement(3488..4972)
                     /locus_tag="ACHA44_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFM8905653.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(4992..5453)
                     /locus_tag="ACHA44_002402"
     CDS             complement(4992..5453)
                     /locus_tag="ACHA44_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8905654.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(5471..6538)
                     /gene="ulaG"
                     /locus_tag="ACHA44_002403"
     CDS             complement(5471..6538)
                     /gene="ulaG"
                     /locus_tag="ACHA44_002403"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFM8905655.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            6756..7526
                     /locus_tag="ACHA44_002404"
     CDS             6756..7526
                     /locus_tag="ACHA44_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFM8905656.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(7799..8626)
                     /locus_tag="ACHA44_002405"
     CDS             complement(7799..8626)
                     /locus_tag="ACHA44_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905657.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         2613 a         1836 c         1359 g         2863 t
ORIGIN      
        1 cgtactcgtt cggcaaaagg aatatacgag atgaaagtca aaattaataa aaaaaattat
       61 cgcatagcat ttgctttaaa aaaaaatcat gtgttcattt tttatattag ttcgaatttg
      121 caaaaaattg tatttgataa agaggtgagt aaaaaaataa aatagctgag cattggagga
      181 tagattgagt gatactaagt ctatcctctt gtttttctaa agaactaagc tagaattttt
      241 tcttttgtac gttattatat gttatttgtc attaccaaaa agcacctttt ttcttctagt
      301 atctatgaca taagaaatac cacccatttc ctttacttga acaaatccta ctgattcata
      361 aaattttttt gttttctcag tatcatctgt caataattcg atttggactg gtccataact
      421 agtgatcttc tccaaagctt cattcaaaag cactttgcct attccttggc gctgatacct
      481 cggatgaatg aggatatctt gaataaatgc gatcgtcgca aaatcagtga tccaacgaac
      541 gattccgata atcttccctt ccccattacg ctttatcaga gattcagatt gttcaaatgc
      601 tctagctaac cttgcagtgt cttttgtgta attcgtccaa ccaacgctct tatataattc
      661 agaaatctca tcgatcataa gggaatcaat attcattttt ttcttccttt cttatacaat
      721 tctccctcta ataatacagt tctttgacta tttaatctat cacatataac tctcctgtaa
      781 agattctctc gattccttaa aattcctctt tccttttaga atgcatactt tttgataaat
      841 aaaaaggttg tgataaattt tcttcacaac ctctgtttct ctctatcaag tgttatcttt
      901 taattttcta cccttaccct agcatctcta ttgcccgtag tatgcatact ttccatgttt
      961 gcgaaaataa tgtttatcta gtaaatattg cgggatttca catatattgg ggttgatcag
     1021 ctcgttttct ttggccatca gacaaatttc atctaagatc aaactgtttt ctactgcttt
     1081 cataggtgaa tccccccatg tgaacggtcc atgtccatag acaagtactc cagggacttc
     1141 gtttggatct aattttcttt ccttaaacgt ctcgacaatc acattccctg tatgaacttc
     1201 gtaagcttct cttacttctt ctttcgttaa ttggcgtgta caaggaacct taccataaaa
     1261 agcatcagca tgagtcgtgc cataagctgg caaatcgcgt cctgcttgcg cccacatgac
     1321 ggagtgcgtg gaatgagtat gagtaatagc agttatatct tcaaaagttt gatacaaaac
     1381 aacatgtgtg gggagatctg aagatggttt taagctatct tcttctatta tatttcccga
     1441 caaatctgtt accaccattt gatctgcttg catgcattca tatctgacac cgcttggctt
     1501 aatgacgatg actcctagct cacggtccac ttcactcaca ttgccccaag tcaatttgac
     1561 taatccgtat cgcggcaatg ccagatttgc ttcaaggaca cgttgcttca ttgctgcaat
     1621 tttttcttca caagacatca tagccagcct ccttcaattt agaacttaag tagtcttttg
     1681 cctgctggat ttctcttcga aagtcttggc ttttttcact ccacatttca atcagaaatg
     1741 ttccatcata atctagacgt ttcaaatgtc ttaataatcc aagaaaatca acacaaccgc
     1801 taccaaaagg aacatcacga aactggcctg aatattcttt tgttacaggg taagtatctt
     1861 ttaagtggat agcagtgata taatcgatcc ctttctcaag ttctcgagcc ggattatttt
     1921 ccggccatgc ggacaaattg cctaggtctg gatatacttg tagaaaaggt gaatgaatct
     1981 gttctttgat ttctaaaaat tttgaaatgg agttcataaa tggatcatcc atgatttcca
     2041 cggaaagaat gatgccatat ttagaagctt cctttacgct ttcttttatt ccttcgataa
     2101 agtcttctct ggaattcatt gatttctctt cataataaac atcgtatcca gctatttgga
     2161 ttactttgac gcttagatta tgcgccaaat ggattgcttt tttcattagt tgtttcgctg
     2221 cttttttctt ttctggatca gcagaaccaa acggaaatcg ccgatgcgca cttaaacaaa
     2281 tcgaatggat acgtacaccg gagctgaaca tctcttctct cagctcagct atcttttctt
     2341 cagaccaatc taatcgtgct aaccgctcat ccgtctcatc aatcgacatc tcgataaaat
     2401 cgaagtccat ttcttttaca agggaaaaac gttctttcca tgaaatatct ttgggtaacg
     2461 ctttttcata aatcccaatt tgagccattt ccttaccccc agatacgaat gatctcatct
     2521 ttgaagtttt gtgcggcctt catcggatca tttgctgctg taataccacg accagtgata
     2581 aaggtataca catcgatccc ttcaaataag tgtaatgtgt gtggatttaa tccaccagtt
     2641 acagaaacat taaagcctag ttcaatcagt tttttgatct tcgataaatc tttttcaccc
     2701 caagtttcac cagataacag cgcatcacgg ctttgatggt agattacctg ccggataccg
     2761 atgttatgcc aatccatggc ttgttcaaaa gtccaattgc cgtagagttc tacttgcaat
     2821 tcacctattt ctttttgtgc tgcctccata gttggaatcg ttgcacaaca aatgactgtc
     2881 atccagtctg ctcctgcttt tgcaacatta ctcgccactg ttcctccagc gtctgcacat
     2941 ttcgtatcag cgactagttt tttatctggg aacatactgc gaatacagcg aatcgctttt
     3001 tgtccttcct gtaagcaaag aattgtgccc acttcaataa tatctactac atcaccaact
     3061 ttcataacat ctcctaatgc atgttctaag tcattgtgat ctaacgcaat ttgcaaattc
     3121 ggcctgctca tcttagttcc ttcctttact tgtttttttg gtaccacttt gtttcagcaa
     3181 atttttcact gatttctttg actgacatca catttttgat accgataact gtaactcctt
     3241 ttttctctgc ctgagtaaac atattcaaaa aattgactgg cgtaaaaact acatcgtatt
     3301 ggccagctga actttttccc tctgaaatgg cacaatgatg gattttggta attggtacgt
     3361 ttagttcttt cattgctttt tccacacttc tcatcatcat caagcttgtt cctgatccat
     3421 ttgcacacga tactaaaatt ctcatcttaa tccctccatt atgagtgttg tctaatcaga
     3481 ttttctttta tactctctgc ttttctgcat atgtgtcata gtcttctaca atcaaaaaat
     3541 aaccatcagg atttttccga tattgtaact gcggaatagc caataaacca atgacaacta
     3601 cggcaattcc agcataacct aggactttca tgattactgt aaacaatggc caagctgttg
     3661 cccagtcgaa catcccgata taaccaccgt atgttgacat cccaatccat gtagcgaata
     3721 atgctgaacc gcctacttgt atgattcctg aaagaaacgg gaacaggcaa gcggctttga
     3781 atccacctcg gttattggca taaactgcta taacggcatt atcaaagaac aacggaatga
     3841 aaccggctat gacgatcgtt ggtgatttca ataaaatcag taaaccaatc atcaaaaact
     3901 gtcccaaagc accaaacaag aacccaatcg ttaccgcatt tggcgaacca aagccaaaag
     3961 tagcagctac gtcaattcct ggaacagctc ctggtaaaat cgtgtttgag attccttgga
     4021 aagattgagt tagttcatcg acgaatgtcc gaacacctaa ttgcaagatc gccagatata
     4081 ccgcaaaatt taaagaagtc gtcataatgt agaataagaa actctgccct tcttgcatga
     4141 attgcgcttg aatcaaataa tctttgccta gtacaatcag aatgatccca aagaaaaata
     4201 gcatgagtaa cgaagtagca accatattct cattgaaaat cgacaagaat cctggtaatt
     4261 tgatatcttc aatttttttg tcttgcttga ttgatctatt tttcttttca tcccgctttt
     4321 tcatccactc agctaatttt gcaaatataa agattccgaa catttgctga tgtgccactg
     4381 caaatccggc accttctgtc aattcctgcg tgattcctac tgttaagtta gaagctactg
     4441 tccaataaca acctaaaatc agtcccataa aaatcaatgc ttgtatccta cctagatcag
     4501 gaaagcaaaa cagtaaaatc caaaaagcgg tagctgcttg ttgaacctga acgtttccgg
     4561 tagtgaaaac ggaacgcagt ttcgtatatt tctgaaaacg gactaataag atatttaaaa
     4621 caaatgcaat cagtaataaa atcatcacat ctccaaaggt acggccaaat gtctccatta
     4681 atccagcatc cacagcattt tgtccaaaat aaggatctgt aaccatggca tccaaattga
     4741 accgttcctt tagtccaacc aagattggcc gaaaattatt gaccaaacca ccagagccaa
     4801 cagtcaaaat aaagtaaccg accgttgcct ttaagaatcc agcgatgcat tcatgaagcg
     4861 ggcgtttcag caaaatatag cctaataaca cgataaaacc aatcaggtat gctggctgcg
     4921 tcaagatatt tgttgcaaaa tacgtccaaa tgcttaacaa aaattccccc atcttttttc
     4981 tcctctatca attaattata ttctgccatc acaacttggt aatcatcgat ggagttcgta
     5041 gagatgagac gctcaaccaa tccatctgtc attaataatt cggataattt ttggatattt
     5101 tctagatgtt cttctggatc ttttgctgcg agtgtaaaaa acaaactagc cgttttctcc
     5161 tcattctccc cttcactttc aaaacggatt ggctgtttca acttggtaaa tgaaatagct
     5221 gttccaaaaa caccatcact tttttctgaa gaatgcggca ttgctacttg aggtgcgatc
     5281 acaatataag gaccgtattt ttctacacaa tggatgatct cctccacata cgtttcatca
     5341 ataatttcct tatcgatcaa tacaagacag ctttcggcga tagcttcacg ccaatccgtg
     5401 atttctttat ctgggtaatg aatcaattga tggtcaaaaa agtatttcag caagaaactc
     5461 cccttctttc ttataaaaac gacggaaacg gtgtatcatt ttcaatcgag aagacatctt
     5521 caaatcctcg ataaaaatta aactcaagat catctttgtt tgtcggatag atgaatttcc
     5581 cgcctacttg ccagatataa ggtttaaatc catacttcag gcgatccttt ttgaatttcc
     5641 aaatttcagt gatctctttt ggatcagcca taaaattaga ccaaatatcg tagtggacag
     5701 gaatcactac ttttgcattc aatgactccg ccatgcgcag catatcaaca gaagtaactt
     5761 tgtctgttat ccctcgtggg ttttcaccat aggcccctaa acagacatcg attttgtgtt
     5821 cattaccatg tttggcaaat aaattggagt aatgtgaatc tcctgcatga taaagattcc
     5881 ctcctgatgt ttcgaataaa tagtttactg caatttgatc catctcctga ggcatttttc
     5941 cttttaatac ctcttctgga tcttcacaag tgaccaacgc tgtccgatca aacgcttcta
     6001 aagctactac attgatgtct ttgattttga ctgcatcacc tggctttact gtaatcgttt
     6061 tttcttcagg aattccccat ttttgccacg tgcgaaccac ttcttttggc ccaatgaatg
     6121 ttgcttcagg acaattttga tgaactgctg cggctgtatg gatatctaga tgatcggaat
     6181 ggatatgtgt aacgattaaa gcgtctacat tttttatcgc aaaaggatcg attacgaaag
     6241 gctgagtacg taaattaggc tgcatatttt gaacgccact catacgcatc atctgatggc
     6301 cttttttcat catgccattc ccatgactac gttttcccgt cccacaccat agatcacata
     6361 aaatattcgt tccttcatga cttttcagcc aaattcccgt acagcccagc caccacatag
     6421 caaaagttcc aggatccact acttctgttt cgatttcttc attcaagtat gtgccccact
     6481 caggaaatgt ttctactaac cacttttctt ttgttacttc attgacagtt ttactcattt
     6541 gcttgctccc cccatatcgt tgttccaact tacaagtaaa tattaatgtg tatcagcatc
     6601 catgaaaacc cttttatatg accgtttgtt ttttatatga ttttctttca gtaatcaatg
     6661 attgtttcag tataataaag acagatatga atgtttaaat gaacaactag tcaaataaat
     6721 ccatggatca agatgtccct caggaggcaa aagtcatgaa aaactcactt gtcaatattg
     6781 aaaaacgtca aaaagaaatt ttggaactgc ttcagcagac tgaacaacta tcaactttcg
     6841 aattagctga atcccttgat gtatctatta gtacgatacg aagagattta aatttattag
     6901 aagaaaaaaa cgagatcata cgcaattacg gttattgttc ttataattac aaaaaccaga
     6961 ctgattttga tcaatctggt cctgaaagga ttaaacaagc gattgccaga gaagctagta
     7021 aatacattaa cgattatgat acgctgttta tcaattctag ctcgactgcg ttgaaaacac
     7081 tggattttct tcaatctaac catttgacag tcattacaaa caacgtgaaa atcaattact
     7141 ctccgcacaa agataattgt agttatattt taactggtgg agagatgcgc tttccaaaag
     7201 aagcactagt gggcgacatc gcattaaata cgatttcttc agtcaatgca gacatttgta
     7261 tcattggctg tagcggagtc gatgtagtac atggggtcac aacaaagata ttaaatgaat
     7321 caacgatcaa cgaaatgatg gtcaacaaga caatcaaatg taagatttta gtcgctgatc
     7381 atcgaaaaat cggactgaca tcaaagttca agattgctga tatttcagtc tttgattatt
     7441 tgatcactga taaattttgt tctgaaaaag tattagagga gatcagaaat acggggataa
     7501 aaaccattca agtagattca ttttaagaac caggacgtaa atatgtaaat aggattatca
     7561 aaaaaatgaa taacaaattt atcgataaat gaaaaacacg ctacgctcaa attgataaag
     7621 gaatcccaag atgtatccat ccaatcgata ccatcataat ctattgcttc ttttataacc
     7681 gttatatgtt gcctaaatag gaatcaaaca atatcgaaaa aaggaggtat gacaaaagtc
     7741 acacctcctt ttttcgaata aacggtattc aaatcagaac actgatttca caaccacttt
     7801 atttcaggtc gttttcaatc acctttaccg cgtaatcatt tccgttgaaa cgcgctattt
     7861 tttttgcgtc catatatctt tctaaataat catcttctcc taattgctta tggcaagaag
     7921 caatttcata aagcaagtat tccaatgcat aagtcgattt atgtttctta ttatactcaa
     7981 tacccttctc acataattgg attgctttat cataatcctc gattttacgg taataggtgg
     8041 cgatatcata atatacaatg cagaccatca gattatcaaa atcagtctca ctcaatattt
     8101 taagtgtacg ctccacataa actttcgcaa aatctaattc tccttttgac aagtagatca
     8161 ttgccacgtt tgcagctgct tgtaagtgat ggatatctgt ttcggtcgtt tgttccaaca
     8221 cttgattaaa ataaaataat gctgcatctg gttttttcgc ataaagatga tcaagcagtc
     8281 cttgataaaa taaaatttga tttaaaagat cagaattact tactttttcc tgatcgatcg
     8341 tttgtaaaat tttataagat tcttggtatt ttgatacaga attcaatgat tgaatctcat
     8401 ttattttttt cctgattttg tcttcttctg tttctactaa tatatcggag atctcgacat
     8461 tcagcttttt gcaaagcgca cttaagatat tgatgcctgg cgacatattc ttgttttcga
     8521 tattgctgac agttgcttgt tcacaaatgc cttctgctaa ttgtacttgt gttaacccct
     8581 ttttctttcg gtattctttt agtaatgctc catctacttt aatcaaaaca atttccttcc
     8641 ctttaaaaaa ttatttctcc tttatatcat a
//
LOCUS       DAYYGS010000098         8281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000098 DAYYGS010000000
VERSION     DAYYGS010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.098"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="ACHA44_002406"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="ACHA44_002406"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFM8905658.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA44_002407"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="ACHA44_002407"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HFM8905659.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="ACHA44_002408"
     CDS             2591..3376
                     /locus_tag="ACHA44_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFM8905660.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="ACHA44_002409"
     CDS             3389..4240
                     /locus_tag="ACHA44_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HFM8905661.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="ACHA44_002410"
     CDS             complement(4364..4816)
                     /locus_tag="ACHA44_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905662.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="ACHA44_002411"
     CDS             complement(5281..5649)
                     /locus_tag="ACHA44_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HFM8905663.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="ACHA44_002412"
     CDS             5799..6281
                     /locus_tag="ACHA44_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HFM8905664.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="ACHA44_002413"
     CDS             6318..7328
                     /locus_tag="ACHA44_002413"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HFM8905665.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="ACHA44_002414"
     CDS             complement(7443..8156)
                     /locus_tag="ACHA44_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905666.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2807 a         1341 c         1680 g         2453 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaagca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtcta tggataggct acaattaact agacagaaaa attaaggtgt gtagactaga
     8281 a
//
LOCUS       DAYYGS010000099         8097 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000099 DAYYGS010000000
VERSION     DAYYGS010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.100"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            382..570
                     /locus_tag="ACHA44_002415"
     CDS             382..570
                     /locus_tag="ACHA44_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFM8905667.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            648..1661
                     /locus_tag="ACHA44_002416"
     CDS             648..1661
                     /locus_tag="ACHA44_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFM8905668.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1728..2762)
                     /locus_tag="ACHA44_002417"
     CDS             complement(1728..2762)
                     /locus_tag="ACHA44_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905669.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            2877..5102
                     /locus_tag="ACHA44_002418"
     CDS             2877..5102
                     /locus_tag="ACHA44_002418"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8905670.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5120..7003
                     /locus_tag="ACHA44_002419"
     CDS             5120..7003
                     /locus_tag="ACHA44_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HFM8905671.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            6984..7958
                     /locus_tag="ACHA44_002420"
     CDS             6984..7958
                     /locus_tag="ACHA44_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HFM8905672.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
BASE COUNT         2614 a         1475 c         1708 g         2300 t
ORIGIN      
        1 gttagtctac attattttgg cgatttgcca ataaggactg ttgagttgtg caaaatcaaa
       61 tctgggtcag aatgaacgtc attctgtctc agatttttcg ttatttcaat ttgattaact
      121 gattgatcaa aaagattttt attttcatat tctcaaatga ttcaacacta aatatacatg
      181 tttgacgcgg aaattattat tcgattttgg cggcatcagg ggggatgacg acgtagcaga
      241 cccattattt tactcaagaa aatgtccgtg cctattattt agaatacgat agtgaacgtg
      301 ctgggagttt tgttctttta gagaaagtct ctgaagataa attagttgtc ttagattttg
      361 actgtaacga aaaatggcga aatggaagag caccagcaag tgattgaccg gatctacgaa
      421 gcgacacgat atctgccttt ggatcgtttg tgcttaagtc ctcaatgtgg atttgcttca
      481 acagaagaag aaaatgtttt gtttgaagaa caatggaaaa aatttctttt gtcaaaagca
      541 ttgcagaaga agtttgggga aaatcattaa atcatctagg agagcttgat aggaaataac
      601 actatggtat aatcagaatg cttaatacta actactgagg gagccttatg aaaaagaaaa
      661 ttcttaaatt tatcggatgg ttcttcggaa tcattcttgg gatcgcgttt gcaatctttc
      721 ttgccttcca gctttccccc agacctggag cattcttgat caaccatatg ttttcaaata
      781 ctgttcaaat cactgataaa aaaacgttcg atcaagcaga aaaaaacgtg gcgaaaaaaa
      841 cagatttgac gtatgagtcc aagtataaag agaatactta tgacatctac tatccaaaaa
      901 acagcaaggg gcctgttcct gttctatttt gggtacatgg tggaggattc gtcggcggag
      961 ataaatcggg tgtcaaagaa tttgcgacaa aattagtttc agacgccaac atcgcagtgg
     1021 ttgcgatgaa ttatgaatta gcgcctgatt cagaataccc taatcaagtt cttcaagtga
     1081 atgaactgat caaagattta ttagctgaag aaaaagacga caaattgcta gacatggaac
     1141 aattattttt tggtggagat agtgcaggtt cacaaatcgc attgcaatat gcagctatac
     1201 aaacgaatga tgcgtatgct aaacaaatga aaatcaaaca gctacttcca aaagatagtc
     1261 tgaaaggaac catttcttat tgcggacctg ttgatttgaa gctaacagca cagcagcatt
     1321 cggatgatcg atttatgaag tttttcgtca aaaccgttgc gtggtcgttg attggaacaa
     1381 aagattggaa aacgagtgag caattgaaag aagctagtgt ggctgaccat gtttctaagg
     1441 aatttccgcc aacgtatata actgacggaa atgcatactc attccaggag caaggtcaag
     1501 cattagaaaa tcggttgaca gaattaaagg tacccgtgca atccttgttt tatacagata
     1561 caaaaaaaga gattacccat gaataccaat ttgattacac gctaaaagaa tcgcaaaatt
     1621 gttaccagca gacattggat tttgtgaata agtataagta agaggttgtg aaaaagccgt
     1681 ttcgttttga gtatgaagaa acgaagcggc ttttcacaac ctctctttca ataccttttt
     1741 gttcttggga taaaactttc cctaacaatc aatcgtgcag gataagtcaa aatgattggt
     1801 tcgtcccgta taccgtcaat tcgctccaat gccattcgaa cagctgcttg cccaattgat
     1861 ttcgcattga tttgaacact ggtcaagcca ggggagaggt ttgcgattgt ctcgatatta
     1921 tcaaagcttg ctacaacaat ctcttctcca atttggatat ttttttgttt caatgcccga
     1981 tagacaccaa ttgccattgg atcgctcccg acaagaatgg cagtaggaag aggggattca
     2041 ttttctaata gattagtcgc aagtacctca ccttgttttt ctgtccagcc acctagatga
     2101 taagacatat atttttctaa gtgatgatcg atcataaaat gctgataggc acgcatcctc
     2161 ttttcattta tagaaatctg cttcccaccg aattcatcca tatcgacttt atatcccccg
     2221 atataggcta ttcttttatg accattttct aagaacaatt ccagtacggt tttagtcatc
     2281 cgttcaagat ctgcatacac catatcgacc tcttgctgga tgtcagggtt atccacgacg
     2341 actatttgca tgttttgttt taacaaggcg gtaatcgatt ctttcgtaac agtcccaaca
     2401 acgatcaccg cacctaactg atctagatct tcccattgtt taggattatc agatagatta
     2461 tagattcgat tcatccctaa atgcagacgt cgagcttctt cttccatccc tactcgcaaa
     2521 tgtttgtaat agatatcctg ttcttcgctc gctttgccga tcgtagagat cacaccaatc
     2581 gaaggcattc tctttttgat atatttacga gtactgatgt cataatccaa cgcagcagct
     2641 gcctctacta tccgttgccg agtagtctga gccacatgga tcgtctgatc gttattcaac
     2701 acacgggaaa ctgtagcagg agagacattt gcctttcgtg cgacatctct aattccaacc
     2761 atttttacac actcctcaaa aaaataaaaa atttttattg acaaaattgt ttagtaaaca
     2821 gtatacagga attataaaaa aaagtaaacg gtttccgttt tgttgggagt gaaatgatgg
     2881 atattacgca tagaaaaaca gataaaaatt tcatcttatt tgatgaaaag agccgagtct
     2941 tccatttacg aaatacattt ctttcttatc tgataaagat agaagaaagc aatgtattgg
     3001 cgcatattta ttttggcaaa ccagtcaagc agtacaaaga caataaaaat tatccccgaa
     3061 gagatcgtgg attttcagga aatgttccct taaatcctga ccggtctctg tcgaaagata
     3121 cactaccgca agaatactct tctcatggca gcatggactt tcgaacgccc gcatcgatca
     3181 ttcagcgcaa gaatggatca gacttactgg atctgagata tgacagccat tatattacag
     3241 atgggaagcc agagatcgaa ggcttgcctc aaacttatgt gatggacaag tcagaggcac
     3301 aaacgttggt gatttcactt aaggatagag aaacagcgat ttatttcgat ttgttctata
     3361 ccatatttac tgatagagca gtgatcacac gatctgtaaa gataagaaat gaaaccggag
     3421 aaacaatcaa actagaaaaa gcggctagtt tccagctaga ctttgcccat actaggcgat
     3481 ttgatgaagt cattgctttg ccaggagcac atgtcaatga aagacagatt tcacggcaat
     3541 ctgttttatc tggaacaaaa gtatttgaaa gccgtcgagg aacgagcagt caccatatga
     3601 ataactttat tgctttagtt catcatcata cgacagaaaa tactggagaa gcgatcggac
     3661 ttcaatttgt ttattccggc aaccattctt tcgaacttga aaaagatcag atcaatcaat
     3721 tgagagtcgt cggtgggatc aacagccatc gtttttcgtg ggaattgaat gcaggccagt
     3781 cttttcaaac acctgaaatg atcctttctt actcaagtca gggattgaac aaaatgagtc
     3841 agatccacca tgagttgcta cgtgaaagaa tcgcaagagg tcgtcatcaa tttgctgaac
     3901 ggccgattct ggtcaataac tgggaagcca cttactttga cttcaatagt gaaaaaatca
     3961 aggcgatcat tgatgaagca aaggagctag gcatcgaaat gttcgtatta gatgatggct
     4021 ggtttggaaa aagagatgcg gacaattctt cattaggaga ctggttcgag tatgaaggca
     4081 agttgacaaa tggattacgt gaaatagcag attatgctca tagcaaagga ttgaaatttg
     4141 gtttatggtt tgaacctgaa atgatctcgg ttgattctga actgtatcga acacatcctg
     4201 attttcttat gcaggagcca ggtagaatgc cttctgcttc cagatcacaa catgtactgg
     4261 attttacaag gttagatgtt cggcagacga ttgagaaaca aatgagaaag attttagata
     4321 cgattcctct tgactacatc aaatgggata tgaaccgttc gctttcagat gtctattcga
     4381 ttacccttga cccacagaga caaggagaag tagcgcatcg ttatatgctc gggctttatg
     4441 aactacttga acatttgaca actgattatc cggaaatctt atgggaaggc tgttctggtg
     4501 gaggtggacg gtttgatgcc gggtttattt attatatgcc gcagtcatgg acaagtgata
     4561 acacggatgc agtggagcgt atgaaaatcc aatatggaac aagccttgct tatccgattt
     4621 cgtccatcac agctcatgta tcagctgtac caaatcatca gaccgacaga tctacttcgt
     4681 tgaaaacaag aggagaaaca gctatgagcg ctgtttttgg ttatgaactg gatttgacaa
     4741 agctgtctcc tgaagaaaaa aagcaagtaa aagaccaaat tatatcctat caaacgattc
     4801 gtccggtgat ccagtatggc cattattatc gtttggcgag tccttttgaa gaaaatattg
     4861 ccgcatggat gtttgtgtct cctaaacaag atgaagctat tgtgttttta gggaggatac
     4921 tagcttcagc tcaaccagcg ttccatgaag tatatctgat ggggttagat gatgaggcac
     4981 tttatcagga acagacctcg aagcggatat tttcgggggc cgaattgatg acagttggac
     5041 tttacttccc cgattttcaa ggtgatttcc aaacagaact gcttcatttc aaaaagttat
     5101 gagagagaag gaaaaaagta tgaaagcaaa tataaaatgg ttagatgatc cacaagtttt
     5161 tagagtgaac caatcaaatg cacatagcga tcatgtttgt tatgcaacaa gggaagaagt
     5221 agaaaccaca tcctccctta aacagtcttt aaatggatca tggcgttttt gttattcgcc
     5281 aaatccttcg gtacgaccaa aaacatttta taaaaaagat tttgatgcct gttcttttga
     5341 tgaaatcact gttcctagtc atattgaatt agcaaattat gacaagatcc attacatcaa
     5401 tacgatgtat ccgtgggagg gacatgtatt tcgccgtccc cctttcacga caagccctga
     5461 gaaagaggcg gaagggatct tttcagctgc taaagacaat ccggttggtt cgtatttgac
     5521 gacttttcga ttagaagaag gtctaaaggg aaaacaaatc tcgattcatt ttgctggtgt
     5581 agaacaagca atgtttattt ggttgaatgg tcattttatc gggtatgccg aagattcgtt
     5641 cactccgtct gaatttgatt taacacctta tttggaagaa gaaaatattc tagctgtgga
     5701 ggtttacaaa agaagtacag ctgcattctt ggaagatcag gactttttcc gattttttgg
     5761 gatcttcaga gatgtcttct tagttgctcg aggacaaaac catttagaag atctttggat
     5821 caaacctcgc ttagatgaca aatgtcaaaa tggcaaactt gaagtagatt tgtcttttga
     5881 gcaagtagcc aaaaacagtc agctgaatgt agcgatacta gatcctgatg ggaaggaaat
     5941 ctatcaacag caatatccgt taaaagagca aatgacgatt cgcatcgatg gatttgaaaa
     6001 tgtggcactg tgggggcatt ttcagcccaa tttatataag cttgaattgg agacgataga
     6061 cgaagaaaaa agagtaacgg agtatattga ctattccttc ggttttcgta aaatcgaaat
     6121 caaagataaa atcatctact tcaatggaaa acggatgatt atcaacgggg taaatcgaca
     6181 tgagtggcat ccgaaaaaag gacgcgccat cactatagaa gatatggaaa aagatttggc
     6241 tatcataaaa aatgcaaaga tcaacggcgt acggacatct cattacccca accagatcac
     6301 ttggtattat ttatgcgacc aagcaggaat ccatgtgatg gcggaaacga acctcgaatc
     6361 ccatggctca tggcaaaaaa tgggcgcatt tgagccttct tacaacgtac caggtgcagt
     6421 acctcagtgg caggcggctg ttcttgatcg agcaaaaacg aatttcgaaa cattaaaaaa
     6481 ccatccaagt attctttttt ggtctcttgg aaatgaatct tatgcggaag aaaacattcg
     6541 acaaatgaac gattacttca aaaaagtaga ccccgatcga cttgtccatt atgaagggaa
     6601 ttttccaaat ccagcattcg aagattcgat ttcagatgta aaaagctgca tgtatgcgcc
     6661 accagaagag atcattgact accttgaaaa taaagcggaa aaaccgttca tcttatgtga
     6721 atatatgcat gacatgggga attcgttagg tggaatggat tcttacatga cgttgcttga
     6781 tcgttatgaa atgtaccaag gaggctatat atgggatttt aaagatcagg cgatccaagt
     6841 gactgatcca atcacaggca gagaagtgat gcgctatggc ggtgactttg acgataaacc
     6901 aagtgactat gaattctcag ggaatgggat cgtttttgca gatggacaag aaaaacccgc
     6961 catgcaggag gtaagatatt attatgaaaa atacagtaaa taaaagtcat atggatacgg
     7021 aaaaagttgc aatcgtcttc ggcgactgta cattaggtgt caaatcgggg aatacgcatt
     7081 atattttttc ttatacaaga ggcggactgg aatcgctcaa taaaaacgga aaagaatggc
     7141 tataccggga aacacttcca gttttttggc gagcaacgac cgataatgac agaggtagcg
     7201 gatttggatt taaatccggt atgtggttag gagccggatt gtatccaaaa gtcaaagaaa
     7261 tagaagtact ggtggacaat aaaaagatcg atttgccgat tgctccagta aataatcaat
     7321 attcaagtaa tgaatacgct gaaacggttg aaatcatttt tacactggaa atcaacacag
     7381 taccaaaaac ccaagtgaca atttcctacc aaatcgctgg agagggtaaa atgcttgtca
     7441 atatgcatta ttatggacaa aaaggattac cggaattacc cgcattgggt ttaaggttca
     7501 ttatgccaac gaaagcaaaa ttttttgagt atgaaggcct atcaggggaa acatatccgg
     7561 atcggatggc tggtggtgtc gaaggtattt ataaagtggc aggactcccc gtttccccat
     7621 accttgttcc ccaagaatgc ggcatgcata tggaaacgaa atgggtagaa ctaacaagaa
     7681 atcaaacgtt ggataatacg gataaagatc aagaagattt ttcgttgcga tttgaacagc
     7741 aggatacgcc atttgctttt tccgctttac cattcaaagc agaagaactg gaacaggcaa
     7801 cacatataga agaactaccg ccagaaagac gaacggttct tacgattttt gcaaaagtaa
     7861 gaggagtcgg gggtatcaat agctggggag cggatgttga gtcaccttac cgaatcagca
     7921 gcgaagagga ccatgaaatc ggctttatta ttcagtagca atctttagat tattatttga
     7981 aaaacggagt ttacaagaca aagctgcttt attctatatg tcatgaacaa gagagtaaaa
     8041 aagttctcag atacttttgt tcatgactat tttcttcaat aaaaccaatt gttattt
//
LOCUS       DAYYGS010000100         8039 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000100 DAYYGS010000000
VERSION     DAYYGS010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.101"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..380
                     /locus_tag="ACHA44_002421"
     CDS             3..380
                     /locus_tag="ACHA44_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905673.1"
                     /translation="MTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKRKLSHG
                     YFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGNICPGF
                     MDQIIENESIESIENYVPVDLPK"
     gene            complement(553..756)
                     /locus_tag="ACHA44_002422"
                     /pseudo
     CDS             complement(553..756)
                     /locus_tag="ACHA44_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            722..919
                     /locus_tag="ACHA44_002423"
     CDS             722..919
                     /locus_tag="ACHA44_002423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905674.1"
                     /translation="MKDSIVEPEGMENTFELYNKTTVDKLIPILVPTDLNGHDVLLRF
                     NGFLGEDYRYLKISDPQPLTE"
     gene            complement(962..1537)
                     /locus_tag="ACHA44_002424"
     CDS             complement(962..1537)
                     /locus_tag="ACHA44_002424"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFM8905675.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1692..2218)
                     /locus_tag="ACHA44_002425"
                     /pseudo
     CDS             complement(1692..2218)
                     /locus_tag="ACHA44_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2675..4186
                     /gene="gadC"
                     /locus_tag="ACHA44_002426"
     CDS             2675..4186
                     /gene="gadC"
                     /locus_tag="ACHA44_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HFM8905676.1"
                     /translation="MNKKKLSLFGFFALTASMVLTVYEYPTFATSQLHLVFFLLLGGI
                     LWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAIFFQWFQITVGFITMIYF
                     ILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTNRTAKIAKAGFVIGIVIP
                     SIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFVSFILAYMGVEASASHIN
                     ELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLSLSAGVIQTFETLILHFN
                     SHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGLLPVFFRKTNKQDVPVPL
                     IMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGYLLFFLGYFVLVFKKKDL
                     KRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAASEHRTYQIILLISFVVTV
                     LLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEHHVTKNDEHILKH"
     gene            4199..5599
                     /locus_tag="ACHA44_002427"
     CDS             4199..5599
                     /locus_tag="ACHA44_002427"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFM8905677.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(5764..7199)
                     /locus_tag="ACHA44_002428"
                     /pseudo
     CDS             complement(5764..7199)
                     /locus_tag="ACHA44_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7592..>8039
                     /locus_tag="ACHA44_002429"
     CDS             7592..>8039
                     /locus_tag="ACHA44_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFM8905678.1"
                     /translation="MNKDITFPVMDVRLVPITKIQANDYNPNRVASPEMNLLELSIIE
                     DGFTQPLVCYYKEKEEKYILVDVLHRYRVAKERLNLKVVPVTVIDKPIEERMASTIRH
                     NRARGTHEIRGMEIVIQRLVKNGWSDKRISKELGMDIEEIFRFKQVS"
BASE COUNT         2564 a         1330 c         1449 g         2696 t
ORIGIN      
        1 gattgacgtg ttctaattgt ggtacattat tttactatga ggaagttaga aatattgtaa
       61 aacatagaaa aaaagaaatg cctatacgtt gtccaaattg caatgaagtt attaagcgaa
      121 agttgtctca tggttatttt gtcagctacg aactatatga agaaagtaaa ataaatctga
      181 aaaaaaaacc aaaatactat aaaaatttga aaactggagc tattataact gaaaaagaat
      241 ttaaaaattt agtgactgaa gaagaaagca ggttttataa tttgtctggt aatatttgcc
      301 ctggatttat ggatcagatt attgaaaatg agtcaattga atcaattgaa aactatgttc
      361 ctgttgactt accaaaatag aaaaaataat cctgttttta cagtgaatta ttttaatttt
      421 gattttcaat aaaaatatat tacaataata atcgtaatag ctaatgaaac aatttctcaa
      481 tctttttacc actccaaaca gggagcgtca ataattttgt gtaaataact cgtccttctg
      541 caaaataatg ggttactcag taaacattga agctaatgta tccgtcactt gttggaagcc
      601 tttatggctt ctatttaaga atttttgatt gtatgtatca aagatggata ctaggaaacg
      661 ctctagtgat tcttcatttt gaaactgctc ttttctgcgg ctgtatttct tgatttgttt
      721 attgaaagat tcgattgtag aaccagaagg aatggagaac acttttgaac tttacaacaa
      781 aactactgtt gataaattaa ttccaatttt agttccaact gatttaaatg gtcatgatgt
      841 tttacttcgt tttaacggat ttttaggtga ggattataga tacctaaaaa tttctgatcc
      901 tcaaccttta acagaataat aaaaaacgtg taatcaatta tttgattaca cgttttttat
      961 ttcaatcttc gttgtagtag tggttgcgac ataccgaaag gtaatcttca ttacctccta
     1021 ctacaatctg ttcacctgaa taaactggaa taccatcaac aatacgagca ttcattacag
     1081 cctttttatt gcaaaaatgg caaatagttt taatttcctc aattttatca gcaaatgtca
     1141 ataatgcttc ggatcccttg aagagattat tttgaaaatc attcttcaat ccatagcaaa
     1201 ttactggtac ttcctcattc attacgataa gtgatagatc aataatattc tctctactta
     1261 aaaattgaga ctcatcgacc aaaatacaat caacttttcc gtactgatca ataatctcca
     1321 ctaattctcc gtacacatcc atttcagaag taattgcaat agctggtttc gaaacaccta
     1381 tacgactctc aacttttcca aatcctgatc gagtatctat tgcgcttgtc attatgataa
     1441 ctctcttccc ttgttcttca taattatgag ccactttcag tagctcaata ctctttccac
     1501 tattcattgt tccatatctg taaaataatt gtgccattaa atttattccc ttcttttaca
     1561 attcaattat tccttaattc catctgcttt tatgcagctg ctcgtttatt ccttgatatg
     1621 tctataacat ctactatccc taaaaccaat ttataagcca ttagagatgg tattcctgaa
     1681 catctctcat attgtttttt gagtggtata tcatctgaaa aactgaagga atcatgaaaa
     1741 tagcgacata cttatcattt agagttctaa ctttaaatgc atttacttct tcagtcagca
     1801 ctttagtcat gttggacatg gtttggggcg tgtagtgatg cccatacatt ttctcaatca
     1861 agtctgcgat ttccgacatg gtaatacctt ttcggaagag gtgaatgacg gtctcctcta
     1921 atgtgtcatt cgtccgtcta taagcaggaa cagtctgttg cttgaactcg ccattgcggt
     1981 cgcgcggaat ctggagatga agttccccat actcggtctt gaccgtacgg tcataggagc
     2041 cgttacgaga attacccgtg ttaaaaccaa tgcgatcgta cttttcgtaa tcgaggaaag
     2101 ccgttaactc cgttttgaga agcgtattga cagctttttc caaatggaca cggaataatc
     2161 cattcaaatc gcctttgttc gctagagtct ttaggatttc tgtagtaaaa tcgttcatat
     2221 ggaagtcctc ttttctgtga atgtgttgtg gtaactctat tctacagaag ggacttcctt
     2281 ttttctattt acacaaaata ttttacactc tcaacaaaag tatcttcgat taatacttgc
     2341 tcaagttatg tgcagctacg aaaaagtagg taccgaggta ctttcttttt cgcagctttt
     2401 tttggactta tgagaagtac attcgctgga agaatacgat ataaaacgag ctttttcact
     2461 aaagatatta atagagtgaa agaaacaagc ttttgttgtc agttttttca cttttctaaa
     2521 aatgaaaata aaaaattctg gaagtggaga agaattttgc ttgctatact ttgtcttgta
     2581 gaaaagaggt agacaaaata atgcaagctc aaataagcga ttttccataa ccttggtagg
     2641 aatgacaaat taaataggaa agggaagtaa taatatgaat aaaaaaaagt tatctctatt
     2701 cggttttttt gccctgacag cttccatggt tttgactgtt tatgagtatc cgacctttgc
     2761 aacatcacaa ttacatttgg ttttctttct tcttttggga ggtattttat ggtttctacc
     2821 agttgcttta tgtgctgcag aaatggcaac agttgaagga tggaaagatg gtgggatttt
     2881 cagttgggtt agtcaaactt taggagaacg ttttggtttt gcagctattt ttttccaatg
     2941 gtttcaaatt acagtaggtt ttattacaat gatctacttc atactaggag cgctgtcgta
     3001 tgtaattaat tttccagagc ttaatacaga tccatttatc aagtttattg gtgtattagt
     3061 cattttctgg gtactgactt tttcacagtt gggcgggacc aatcgtaccg ctaaaattgc
     3121 taaagcagga tttgtcattg gaattgttat tccttcgatc attctctttg gtttagcagc
     3181 agcctacttt atcggcggta atccgattca aatggctatt aatgcgaaag cttttattcc
     3241 tgacttttct cagctttcaa ctttagtagt atttgtatct tttatattgg cttatatggg
     3301 cgtagaagct tcagcatcac atattaatga gctggaaaat ccccaaagaa actacccatt
     3361 ggcaatgttt ttactagtga ttttggccat cgccttagat gcaatcggcg gtttctcagt
     3421 agcagcggtt attccacaac atgacttgtc tctcagtgct ggtgtaatcc aaacatttga
     3481 aacgcttatt ttacatttta acagtcattt acattggttg gtaaaagtga ttgctctcat
     3541 gattgcctgt ggcgttatgg gtgaagttag ttcttgggta gttggtccat cacgagggat
     3601 gtttgcagct gctcagcgag gtctgttacc agttttcttc cggaaaacta ataaacaaga
     3661 cgttccagtt ccgttgatta tgatacaagg aattatcgtt actatttggg cagcagtctt
     3721 aacttttggt ggaggaggca acaatttatc attcttagta gcaatctcct taactgttgt
     3781 catttatctc gtaggatatt tactattttt cctaggttac tttgtattag tttttaagaa
     3841 aaaagactta aaacgaactt acaatgtacc aggaaaaact gttggcaaaa cgattatcgg
     3901 tggaattggt tttattttat cagtgttcgc attgcttatc tcttttgtac caccagcttc
     3961 aattgcagct agtgagcacc gaacatacca aatcatacta cttattagct ttgtagtgac
     4021 tgttctttta ccttttatca tttatgaagt acatgataaa agtgctcaca caactattga
     4081 ggaacctact cacgtgaaag ctgaagacgt taatcccgct gtttatccag ctgctagagg
     4141 agagcaccac gttacaaaaa atgatgaaca tattttaaaa cattaggagg aattcattat
     4201 gttatacgga aaagataatc aagaagaaaa aaactatttg gaaccaattt ttggctctgc
     4261 aagtgaggat gttgacttgc ctaaatataa gttaaacaaa gaatccattg aaccacgaat
     4321 tgcttatcaa ttagtacaag acgagatgtt ggatgaagga aatgcgcgat taaacttagc
     4381 tactttttgt caaacgtata tggaacctga agcagtgaaa ttgatgaccc aaacgttaga
     4441 aaaaaatgca attgataaat cagaataccc acgaacaacg gaaattgaaa accgctgtgt
     4501 aaatatgatt gctgatttat ggcatgctcc aaataatgaa aaattcatgg gaacttcaac
     4561 gatcggctct tcagaagcct gcatgctggg tggtatggcc atgaaatttg cttggcgtaa
     4621 acgtgctgaa aaattaggtc ttgatattca agcaaaaaaa cctaacctgg tgatctcttc
     4681 tggttaccaa gtttgttggg aaaaattctg tgtatattgg gatgtggaac tgagagaagt
     4741 cccaatggat gaaaaacata tgtcaattaa tctagatact gtcatggatt atgtggatga
     4801 gtacacaatt ggtattgtag gtattatggg tattacttac actggtcgtt atgatgatat
     4861 caagggtctg aatgatttag ttgaagctca caataaacaa actgactata aagtatacat
     4921 tcatgttgac gctgcatcgg gtggctttta tgcaccattt actgaacctg atctagtttg
     4981 ggattttcaa ttgaaaaatg ttatctcaat taattcttca ggtcacaaat atggtttggt
     5041 atatccaggt gtgggttggg tcttatggcg tgaccaacaa tacttaccag aagaattagt
     5101 atttaaagtt agttacttag gtggagaaat gccaactatg gctatcaact tctctcatag
     5161 tgcagcacaa ctaattgggc aatactacaa ctttgtacgc tatggctttg atggttatcg
     5221 tgatattcac caaagaactc atgatgttgc tgtttattta gccaaagaga tcgaaaaaac
     5281 tggtattttt gaaatcatta atgatggatc agaattgcca gttgtgtgct ataagctgaa
     5341 agaagatccc aatcgcgaat ggacactata tgatttatct gatcgtctgt taatgaaggg
     5401 atggcaagtc ccagcctacc cattacctaa agacttggat caattaatta ttcaacgctt
     5461 agttgttcga gcagactttg gaatgaacat ggctggtgat tatgtacaag atatgaacca
     5521 agcaattgaa gagttgaata aagctcatat tgtttatcat aaaaaacagg atgtgaaaac
     5581 gtacggcttt actcactaaa attcttcaga gaaatataaa agaagttaat taactaataa
     5641 aaggagttgt gattaccaca cagctcctta aatctatttg aataaatata tgatattcat
     5701 ttgttacact ctcaatctta gctgttgttc tattcatcat tactcttatt gtaagtagtt
     5761 cagtcaaatt tgcttgttca attgatcgat aacagtatga attgtttgaa aagtaatcgg
     5821 atatataatt gttttctcac tacctaagcc ttcttggtat aaagaactat cgcaaagtat
     5881 aatccgctga gtacaaaaga aagtgtcgtc tactatttct ctaagttgtt tacaaatgat
     5941 attactaata gaaaagtgtt ctcccaaaat gtcttctaac tgttgtcgat agtataaata
     6001 cctaaagtct gacggagcta ctatgaacac ttcaattgga gatttccgtt tatgctcatt
     6061 taaaagactt aaactaatgg ttagtttacg aatagaattc tcattccaac gcagaaataa
     6121 attgttttgt tttcgccatt gttcaagaat ttcatgaact tctaagtaca aagcgcgttg
     6181 ctcttcattt aacaagtaag tcttatcagg ttgaaatatc tgaatatcag cccaaagtga
     6241 gcggataaac gggagaagtg ctttatcaaa aagtatctga ttttcttcag atatattcag
     6301 agataagata agtttttgac tgagctcaat caccaaaggg ttatctgcta catggttttg
     6361 ataaacaacg ttcaagtctt tttgtaacaa ttcaaagtta tcgcatagat aatttctaga
     6421 gttgaataga aagtagatat aaaggcattc atcttcgtca tgacataaag agaaagaaag
     6481 ttgctctaat cctttctgta ttagctgaaa caagggagta tgtcgggcaa ctcttttttc
     6541 ttcctgacta aaatatattg ggttagtctt agatctctgc aaaccgatag cgatacctaa
     6601 taaaataaat tgtttttcaa ctgtagggta acttcttgaa aaaaatgttt ttctacataa
     6661 tcaatcaatt gttgaccgct ttgaataatt gatttattta gctggatcaa ctctgtccga
     6721 tttgtctcat agattatcgt tagtaacaaa cttcgataat ccttttcagg aatttcaaaa
     6781 tggccatcag catttaagta ctgcatttgt ttaaaaaaat taatcaactc atttcgtaag
     6841 ttgtaaactt tgcttaacga aatgaaggta gtatcagcta tattactcca attgtgttcc
     6901 cctgataaat aatgtcctag tacattgaaa aagtcagagc gctgatagat gagttgagtg
     6961 agtgccaacc tcgaaaacga agagtcgaat gcgactgtaa agctacttct agatttctcc
     7021 actgaaataa tttgctgatg gagtatgtcg gtaatgcatt ccaagtcgtt agtgacagtg
     7081 agtgggcaaa cgttcaattg atctgctatt tcgcgaatct gaatggtccc tttttcgtgt
     7141 aattgctcac aaatgtaaac ttgacgaaaa atacttttct ccagataggt ttccaacata
     7201 gtactcctcc tagtcatttt atacataact attataactc taccaatcgg aatagtcctg
     7261 taatatattg aatcaaaaat ataaattata aaataattag atggggtatt tggtttaagt
     7321 atcccttata tagtaataaa tttataggtg tgattctcga attaataccc gaaaatgtga
     7381 gagaattttt atggattttt taggatacaa attaattatt tttgctttta tcctgattac
     7441 cagtccagta ctgtcggtat cccattttgc agcaaaatca tgttgataaa gtgacaagaa
     7501 gactgatgag atatattatc caaattgaga agataattaa tcggattctt gagtataccc
     7561 tactttttaa gaaagtaggg tatacaagcg tatgaataaa gacataactt tccctgtgat
     7621 ggacgttcgg cttgttccaa ttaccaaaat acaggcgaac gattataatc ccaatcgagt
     7681 agcgtctccg gaaatgaact tattggagct ttctatcatt gaagatggct ttactcaacc
     7741 attagtttgt tattacaagg aaaaagaaga gaagtatatc ttggttgatg tgcttcatag
     7801 gtatcgtgtt gctaaggaaa gactcaactt aaaagttgta ccggtaacag ttattgataa
     7861 acccatagaa gaacgtatgg cttcaacaat tcgacacaat cgtgcaagag ggacccatga
     7921 aataagagga atggaaattg tcatccaaag attagtaaaa aatggatggt cagacaagag
     7981 aatatccaaa gaattaggca tggacattga agaaattttt cgctttaagc aagtgtctg
//
LOCUS       DAYYGS010000101         7991 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000101 DAYYGS010000000
VERSION     DAYYGS010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.102"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            134..700
                     /locus_tag="ACHA44_002430"
     CDS             134..700
                     /locus_tag="ACHA44_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8905679.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            845..932
                     /locus_tag="ACHA44_002431"
     tRNA            845..932
                     /locus_tag="ACHA44_002431"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:877..879,aa:Ser,seq:cga)
     gene            1268..2242
                     /gene="bsh"
                     /locus_tag="ACHA44_002432"
     CDS             1268..2242
                     /gene="bsh"
                     /locus_tag="ACHA44_002432"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8905680.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            2567..4462
                     /gene="asnB"
                     /locus_tag="ACHA44_002433"
     CDS             2567..4462
                     /gene="asnB"
                     /locus_tag="ACHA44_002433"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HFM8905681.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(4657..5103)
                     /locus_tag="ACHA44_002434"
     CDS             complement(4657..5103)
                     /locus_tag="ACHA44_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HFM8905682.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            5331..5480
                     /gene="rpmG"
                     /locus_tag="ACHA44_002435"
     CDS             5331..5480
                     /gene="rpmG"
                     /locus_tag="ACHA44_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFM8905683.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            5568..6086
                     /locus_tag="ACHA44_002436"
     CDS             5568..6086
                     /locus_tag="ACHA44_002436"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFM8905684.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(6163..7119)
                     /locus_tag="ACHA44_002437"
     CDS             complement(6163..7119)
                     /locus_tag="ACHA44_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFM8905685.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            7285..>7991
                     /locus_tag="ACHA44_002438"
     CDS             7285..>7991
                     /locus_tag="ACHA44_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905686.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKK"
BASE COUNT         2628 a         1348 c         1551 g         2464 t
ORIGIN      
        1 tttgcaaaaa aaagttaaag aagactgaaa aaaatttaaa atataacgtt ttgagtaaaa
       61 tgtttgcaaa aaatgctaaa aatagggtac aataaaacaa catatactgc agtaaagaag
      121 tgggggagtt ttaatgagaa gaaaagtcta taccaaggat caaattctaa aagctgctta
      181 tgatgttgtt gcaaaggaag ggtttaaagg atttactgcg agaaatattg ctaaaaagat
      241 ggggatttcg acacagccga tttatctgga attcaaaaac atggaagatt taaaaaatac
      301 gttgctggat tctatctttg aacaactaaa agaagatgtt ttccctattg aacgtactgg
      361 tgataaaatc attgatctcg gtttgaacta tatctacttt gcaaaaaagc atcatagtct
      421 ttacatggca ttgtacattg atgaacatgg ggacggaaag aaaatgcatg atttttcata
      481 caattatttc cgtgaaatag ttaaagaaga tgaaagatac aatcaattat cggatgaata
      541 tgtcgatgct ctgcatactg gtacatgggt agcagtgact ggcttagcgt cattgatgtc
      601 atcaaacatc atgcatccaa cagaagaaca aatcattgca ctgatcaaac ggacaattga
      661 ctcgatttta gcattagata atcctgaaat aatcagatag tgttctatca gtatgaattt
      721 gatcggtaag tccgttggct gcaaactgtc aatggacttt tttgctaaaa aaagcgattt
      781 ctgttattta tctattagaa ataggcttga catttcgtga aaaaagttta aaaatagtac
      841 ttacggaagg ttggcagagt ggtaatgcac cggactcgaa atccggcgaa ccggctaata
      901 ccggcgcgca ggttcaaatc ctgtaccttc cttatatcaa cgattagagg ttgtgaagaa
      961 gctgtcctgc atcaagtaat aagaacggcc aaacaaaaat agcttttcct attttttcgt
     1021 ttggttgggc ttattaccgc agatgcaagc ttttgaacac cgtttgttcg gaataagggg
     1081 ctgtgacaag acttttgtca cagcccctta ttgttgtact tttaacctac aatatttttt
     1141 gattccccct gagtactatt ttttataaat gctagtaaat caatcttctt tggccttttt
     1201 tttgataaaa aaaaatacta gatgtaagat aaaagtgtaa aaacgatttg ttttaggagg
     1261 gaaaacgatg tgtaccgcaa tcacttatgc aacgaaagac cattattttg gaaggaattt
     1321 tgattatgag atgtcttata atgaagtagt cactattact ccaagaaatt atcgtttcga
     1381 ttttagaaag gtaaaaaatt tagataaaca ttatgcaatg ataggaatag cagctggggt
     1441 atccaactat ccactctatt atgaggctac aaatgaaaaa gggttgagta tggcaggact
     1501 gaattttcct ggcaatgctg attataaaga attacaagaa ggaaaagata acgttgcacc
     1561 atttgagttt attccatgga tactagggca atgctcaacc atagacgaag ctaaagaatt
     1621 attggctact atcaatctag tcaatatcga ttttagtgaa aaactaccct tgtcaccttt
     1681 acactggtta ttagctgata aagaaaaatc gatagtaatt gaaagtatga aagatggact
     1741 tcatttatat gataatcctg tcggtgtgtt gacaaataat ccgccatttg attatcaatt
     1801 atttaatctg aacaattatc gctcgctatc aaatggaaca ccagaaaatc atttttcaaa
     1861 ccagatcagc ttgaatgttt atagtcgtgg gatgggagga ttgggcttac ctggagatct
     1921 ctcttctgtt tctcgatttg taaaagccac atttactaaa atgaatgctg catcaggtga
     1981 ttcagaatct gaaagtatca gtcaattttt tcatatacta ggctcagtcg agcaacaaaa
     2041 aggagtatgc gatactggag aaggaaaata cgaatatacg atttattctt cttgctgtaa
     2101 cgttgataaa gggatttatt attatcgtac gtatgaagac agtcaaatta ctgctgtaaa
     2161 tataaataaa gaagacttgg atcgtcatga attggtcagt tatccagtca taaaagagca
     2221 acagataaac tatgtcaatt aattaatgaa tagatccgtt tggtatcata ttttctgata
     2281 cagtcataat tagacacgat ctttaggtcg tgtctttttt tgctgttttt tttggaaata
     2341 gcatccacta atattttttt aacacgaaga gaataaggta gcctcaaaaa agaaaacatg
     2401 gtaaattaga tattgggact ttattttctg taaaaacaga aactttcatc ttttgttttc
     2461 acgtgacgaa attgtaagag agaaaatatt cgtccgctat ttattctatg ataagatgca
     2521 tatgaatgaa ataaacagta attttatatt aggatgtgaa agttaaatgt gcggtattgt
     2581 aggttttgtt aacgacaagg acaacaaaaa aacaattatt aatacaatga tggatcgaat
     2641 cgttcaccgt ggacctaata gttcaggaga atatatcgat aaacatgttg ctttaggatt
     2701 cagaagatta agtattattg acttagaggg cggtactcag cctatttaca atgaagatcg
     2761 gacaaagatc attattttta atggcgaaat ctataattac cagcctttga gagaagaatt
     2821 gattgcagct ggccacgttt ttcaaacaca tgcagataca gaagtccttt tgcatggtta
     2881 cgaagaatgg ggaacagaat tattgcaaaa gatccgcgga atgtttgctt ttgctatttg
     2941 ggataatgaa aagaatgagt tattcggtgc aagagaccac tttgggatca aaccatatta
     3001 ttatgcagaa atgaacggaa catttatgtt tggttcagaa atcaaaagtt ttctgcctca
     3061 tcctgatttt aacaaagaat tgaacaaaga agcgttgaaa ccttatatga ctttccaata
     3121 ttctccatta aacggcgaaa cattcttcaa agatgtctat cgtttgcctg aaggccatta
     3181 ttatacgtat aaagatggaa aactagacat tcagcaatat tgggatgcag attttgaaac
     3241 aaaagaaacg cattcccgac aagaatggat cgaaaaaatc gacgaaaccg ttcaagcttc
     3301 tattgaagca catacggtca gtgatgtaga agttggttct ttcttatcca gcggggttga
     3361 ttccagttat gtaacctctg tattgaaacc agatcattct ttctcaatcg ggtttgatga
     3421 taaaacctat aatgaagcga ttgaagccag aaaactaacc gaactattgg acttagataa
     3481 tacagcagca gtgatcgacg gagacatgtc gttcaaagca ttccctttga tccagtatca
     3541 cttagatgaa cctgattcaa atccttcttg cgtacctttg tactttttag caaatcttgc
     3601 ttcacaaagt gtgcgggtag tccaatctgg agaaggggcg gatgaactgt ttgcgggata
     3661 ccaaacgtat ggattccata cgaattcaaa attcatccgt gtgattgctc aaggattgaa
     3721 aaaattacca aaaggaacac gctataattt aggacgtaaa atcggaaaaa tgaaaaattt
     3781 ccatggacgg atccatttat atgaatcact tgctcctgca aaagaatatt ttatcggtca
     3841 tgctcgagtg ttcgaagaat cagaagcatc agaagtgtta acaccaaaat atcaaacagc
     3901 accatctgtg gatgaaatca tgactgttca ttacgagaaa acagaaggaa tcaaagatga
     3961 agtcaataag atgcaatatg tcgatcttca tcaatggatg ccaaaagata tcttgttaaa
     4021 ggcagataaa ctttctatgg caagttcttt ggaagtccga gtacctttat tggatatcga
     4081 agtcatgaaa ttggctcaac aaataccaag taagttttta ttgaatcaaa acaacacaaa
     4141 agatattttc agacaagctg cgaacaaaca tcttccggaa gaatggtcga atcgtgtgaa
     4201 attaggattc cctgtgccaa tcaaagcttg gttgaaagaa gaacacggct acgaacaagt
     4261 caaagcatta ttcgaagcag attttgcaaa agaatttttt gatcaagaaa aaatcatgaa
     4321 gttgctagat gaccatcacg aaggacgtaa agaagagcaa agaaaaatct ggacaatctt
     4381 cagtttcttg acatggtata aagtctactt tgtagatgaa acgattccgc aagcagaagc
     4441 aatcgactat gtgactgttt gatgaaaatc aaatatcaat aaaaaagagg ttttctccta
     4501 ccgtttaaca ctgagtgata agaaacaatt tcaaaaaaca gctcttcccg ttttatagat
     4561 attatgactt atggttcgga gttggcagag gaacaccatt gtttcgaaca taagggggtt
     4621 gtgacacgat tttcgtcaca acccctttac atatgttcaa taatcgattt ctttttcttt
     4681 tgtcccatct tttttataaa tgaacaaagt tgattctttg ttttgtgcta cttgctttcc
     4741 tttttcgatt gcttcttttt ttgtatcaaa atggttgctt gccttttttg cccctttaga
     4801 tttcacgatc cattgttctt cctcatattc tacgatcaca tctgaatcaa gcagttttcc
     4861 agcacgagga ttcgtatcgt gttcatccgt cttgcttgga tttttttctt tttcgaaagt
     4921 tcgtttttct gattcactag catcttcata ccattttttt gcttgatcaa tggcaattgg
     4981 gatcgcacgg ttatcagggt acccttcatc taataatgca tttgcgatgt cgattgcttt
     5041 ttttcttgtc aatttatcta agtttttcat tgatgcagga aagtccttca tgttccatgg
     5101 catattagta tcccccttta ttaaaaaaca taatctatat aaacaagtgt aagggtattt
     5161 atacaaaaag aaaaagattt tgtttgagaa gtaaaaccgt tgtatttatc gtcaagtagt
     5221 tttacttaac aaaatcgtga aaaacactag caatctagaa aaaatcatgg taagataatc
     5281 aagtctgaga gtcatcttag aggattcttg taaaaatggg agggaaaaac atgcgcgtaa
     5341 acatcacttt agagtgtact tcttgtaaag aacgtaacta tctaacaagc aaaaacaaac
     5401 gtaacaatcc tgatcgtttg gaaaaacaaa aatattgccc acgcgaacgc aaagttactt
     5461 tgcaccgtga aacaaaataa tgtttttaaa gttctcaatt ccttgctggt caaagaattg
     5521 agactttttt tatctcaaaa catctaaaat cataaggagc ggataatatg tttatttcca
     5581 tgtgggcaca agataaaaat ggtctgatcg gcaaaaatgg tttgttacca tggcgattgc
     5641 caaatgatat gcgtttcttt cgcgaacata caatggatcg aattttagtg atgggaagaa
     5701 agacctatga aggaatgggg gatctttcac ttccttatcg ccatatcatc gtgttgacaa
     5761 cgcaaccaaa ttttcgcacg aaagaaaatg cggaagtaat gcattcgatc gatgaacttt
     5821 tgtcgtatag tcaaacgatc tcagaagata tctatgtttc gggtggtagt cggatttttc
     5881 aagaacttct tccttacact ggtcggatct ggcgaacatt gatcgatagc acgtttgaag
     5941 gcgatacgta tattgggaat attgattttt cagacttcgc ccttatagag gagtatgagg
     6001 ggataacgga tcaagaaaat ctatatgccc atcgttttca aaaatgggaa agagtaaaaa
     6061 atagggttgc ttcaaaagaa gaataaaatc tatgcgtcta gtttgttaat aagaaaaaaa
     6121 gcatctcacg actaatcgtg aagattcttt ttttagttaa aatcaattta gtttttcttt
     6181 gatatcattg accatttcct gactggcaat aggtccgtta tatagccaac tagattcagg
     6241 tccgaattgc agtacatgat tgtctgtcac tgctttaaga ttcttccagc ttggttcatt
     6301 gaacatggca gcatctttgt cactgttcac aaggatgatg tagtcagcat ctaattcagc
     6361 tagcttttca gaggaaacag cagaccagtc agaagtggct tttttggaga cctcttccac
     6421 tagatcagga acaccaaatt ccagatcgtc gtatacgatt cttccacttg accgattttt
     6481 gctgaccata aaggctgagt tgttggtcac ccacaacact gcagctgtct tgttctcgat
     6541 tttatctgct agttcttctc gagttttctt gatggatttt tgataatcgg aaatgatttt
     6601 ttctgcctct ttttcttttg ccaaagcttt gccgacatct tttagttgtt cttcccatgt
     6661 gacatctgtt ccgtttttta caacataagt tggcgcgatt ttgctatatt gttcgtattt
     6721 cccgccttcg acagtagcgg aagaagaaat caacagcaaa tcaggctcaa agcttaacac
     6781 tttctcataa ggaaggtcat aagaaatcgt tgggacatcc tttaaatcat cttgcagata
     6841 atcttgaatg gaaccactgc caacagtcca ctgggcgata ggtttttccc ctagtgcaat
     6901 cagatagtct tctaagtaac tgccgatgat ccgtttgggc gcgtttggaa tctctacttt
     6961 atgtccaagt gtatctgtga ctgtatgagt actattggat gaagtcgtcg aattggcagt
     7021 tgtagctgtt tctttatctg aagaggggga gcaagcgctc agtactccag ccagcagcaa
     7081 tatgctagat aatgtgaata cttttgtttt taatttcatt tctttcctcc taacataatt
     7141 gagaatcttt ctcattggtt attattcctg atatttcgaa cgctgtcaac cgcttcattt
     7201 ggctatagat atgaaagtag cgcttgacga aatgttgaaa cagcgtcata attgagaatg
     7261 attttcagcg aaaggatggg tgaaatgacg agattaagag cagctgacat tgcagccggc
     7321 tacgaaaaca aaaaaatcat tgaaaacatg tctgttacga ttcctgaagg gaaaatcact
     7381 agtttgattg ggccaaatgg aagtggaaaa tctactttat tgaaaagttt tacccgtata
     7441 ctacctgtga aatcaggaaa gatcattgtc aataactctg atatctctac atacagttca
     7501 aaagaacttg caaaaaaaat tacattatta gcacaaacta gtgaacaccc tacaggtatg
     7561 acagtagaag aagtagtatc ttacggacga tacccttatc aaaaactgtt cagtggcatg
     7621 gacacgcaag atcatgaagc aatcgagtgg gcgttggatg ctacaggtct agctggattt
     7681 cgtatgcggg atatcgcaac gctgtctggt ggacaaaccc agcgtgtctg gatcgctatg
     7741 gcgcttgctc aggaaacaga tatcttgatc ttggatgaac cgacaacatt tttagatccg
     7801 gcacaccaat tagagatcct acagttattg actgaaatca atcaaatgaa acaaacaacg
     7861 atcttgatgt cgattcatga cattaatcat gcttcacgtt tttcagatta tttattagca
     7921 atgaaaaacg gtaagttgat cacagaaggt acacctgaag aagtgatcac aaaagagtgt
     7981 atgaaaaaaa t
//
LOCUS       DAYYGS010000102         7811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000102 DAYYGS010000000
VERSION     DAYYGS010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.104"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHA44_002439"
     CDS             complement(31..1383)
                     /gene="celB"
                     /locus_tag="ACHA44_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HFM8905687.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(1383..1901)
                     /locus_tag="ACHA44_002440"
     CDS             complement(1383..1901)
                     /locus_tag="ACHA44_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905688.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(1917..2243)
                     /locus_tag="ACHA44_002441"
     CDS             complement(1917..2243)
                     /locus_tag="ACHA44_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HFM8905689.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(2256..4235)
                     /locus_tag="ACHA44_002442"
     CDS             complement(2256..4235)
                     /locus_tag="ACHA44_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8905690.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(4256..4570)
                     /locus_tag="ACHA44_002443"
     CDS             complement(4256..4570)
                     /locus_tag="ACHA44_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HFM8905691.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4738..5031
                     /locus_tag="ACHA44_002444"
     CDS             4738..5031
                     /locus_tag="ACHA44_002444"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HFM8905692.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(5109..6221)
                     /locus_tag="ACHA44_002445"
     CDS             complement(5109..6221)
                     /locus_tag="ACHA44_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFM8905693.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            6374..7222
                     /locus_tag="ACHA44_002446"
                     /pseudo
     CDS             6374..7222
                     /locus_tag="ACHA44_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7575..>7811)
                     /locus_tag="ACHA44_002447"
     CDS             complement(7575..>7811)
                     /locus_tag="ACHA44_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HFM8905694.1"
                     /translation="DATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKH
                     LPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         2495 a         1554 c         1262 g         2500 t
ORIGIN      
        1 tttttgtcac agttcctttt ttcttagaaa tcattttaac ttttcactag ctaattctgc
       61 tgccttttct ctttcttcat ttaataattt attatcatag aatttgataa atgggaacca
      121 gataatgaat gcgatgattg cacaaatgac tgctaacaca gcagctttcc aatcaccacc
      181 actaccaaca aatgctccga tcccgactgg tgaaggccac ggcatctgtg caagcatcgg
      241 tttgacgatt tccagccgaa tggcaaaata agcgagtgta gcagaagcca tcggagctaa
      301 gaaaaacgga accgctaaat agggattgta gacgattggc atcccgaaaa tgattggttc
      361 attgatattg aataatcctg gtacaagtga ggcttttcct aacactttca gctgatcaga
      421 tttcgcgagg aatgcaataa agatgatcaa tcctaaagtc gcacctgatc caccaacggt
      481 tacataagaa ttgttgaatt ctcctgccaa cggataattt gcgccttgtg cgttagctgc
      541 catattagct aatacgatcg gggtcaagaa agaagtgatg atattcgctc catggatccc
      601 tacgatccac aatgcatgga tcaagaaata aatcaccatg acaccaagcc atgtattcgt
      661 caactgcgta acaaacccaa atgggatagc aatcattttg aagatatccg ttcctagtgc
      721 aactaataat ccattgatga tcatcaccac aaacgcaatg acaaatgttg ggatcagagc
      781 agtgaaagaa cgagaaactc ctggcggaac aacatctggc atttttatga cccaatttct
      841 cttgacacac atacaataca attcgaccgc aataatcgac ataataatag ctgtaaagat
      901 tcctgaagtt cctaaacgtt cgacaaaaga gcccatccta ataccattta ctacagtttc
      961 tgtctcattg atagaagcaa tcaagctgat tttaccatca gtcatgatca attcagggat
     1021 acacataaag aatgcaaaaa cagatagtaa tgcccccgtg agcggattaa catttaattt
     1081 ttgttcatct cgttcaattg acgttaattc ataagcaaac acaatggcaa agtaaataga
     1141 taaaatcccc atcgtcattg tattgacgat catgtacaag tcactaactt tgaaaaaagt
     1201 tgcattaaaa aatccttcta aaaatgggaa cggtaatggc aaaatattta aaaccaaaaa
     1261 catcgagcct acaatcgtga aaggaataga agccatcccc gctgccatga cagcccgaac
     1321 gattttaagt tgcgcaattt tccccatagg tcccatcagg tatttctgca aaaaatcaaa
     1381 cattagttat tctcctttcc taaatataac gctttttcat attgtttaat acggatatac
     1441 tgttggtctt ggttgagtga aattttcttt aaacgatact gtataaatgc actggcaaaa
     1501 ataccgctta caacaagaat gaaaacaaat aactgtcctc cattgttatt tgccataaaa
     1561 ggatacaaga atgtaaatcc tgaggtaaac aacactggaa tcactaataa attaaagaca
     1621 ctctggaaca aaaaatacca ctttgtccat ggaacgttcg tttggtgttt tcgataaagc
     1681 tttacttgtt ctcctactgc aggcaaaata gctaaaatca gaatcatcgg taaactactt
     1741 cctaaacttt tagctgatag aagcagaatc atccattgca tgttcatgaa aaagaaaaag
     1801 gcagtcaggt aacgtacgat tagataacga ttgaaataca tgtttttcaa actaagttgc
     1861 tttttttcta attcgatttt cctttttact gtatcctgca caatgatcct tcctttctat
     1921 ttattttttt gatacaaata attcatttcc tgtgcaactt ccaataatgt catcgtcgtc
     1981 atcagatgat cttgtgcatg gaccatgata atttccattt ctatttttgt cccgctggca
     2041 tattctttca gaagttgagt ttgtgactga tgcgcatgaa gaatctcttc atttgcttgt
     2101 cgcaacttct cttctacttc ttcaaacatc tctttacgca tcgagcgaaa tgcttcatgt
     2161 atcagtgtcc gtgcatttcc gctatggaaa atgatatcaa aagaagtcac ttgtagtgtt
     2221 tcactgttta ttttttcagc catctgtttt cctccttatt cgatcagttt gataaattta
     2281 ctgctgaaat tctcaaaaat aggttcagct aagatttcct gctgtacttt tagctggtca
     2341 actaacctaa cgattgcttt agtcacgacc gtaattcctt cattttcgac ataagaaggc
     2401 gaaactaaaa aaacaaaatg aatttcttga tattgatccc actgcatccc agaaggaatc
     2461 aatgcaacgg ctacctttgt ttcgattcca actggaacag cgggatgtgg gactgccact
     2521 gtatcactga atatgatttg tcccatattt tcacgttgct ggatctgctt tcgcatttct
     2581 cttatatatc cttcttcttc atttatgctc agcttttgca gcaaatcatg aagaatttct
     2641 tcctttttcg gttcgtacgt ataagtcttg aaaaattttt cagacaactg ttcggtgtaa
     2701 atctgctttt tttgctgtag agaaaatgct ggtatcggtc tatctgagga atgatagggt
     2761 atagattctc caactacctt tcgtatttta cgaacatcat cttcattcaa aaaaatactg
     2821 acatgtaaaa ctggaatttt gaaaaacatt gtcgaaagat caatagaaga aataatcagg
     2881 tcgattcctt ttaaagttgt ttcattgatc tcataatagc cttttacact taccactgta
     2941 atatttttac cgaattcact gactacgcga ttctttaata gttgggcact gccgtaaccc
     3001 gttgcacaga taatcaatgc atgtagttta tgcgcagctc tttctttttc caatgccgcc
     3061 atgagatgca aagccaaata ggcccattca tcttcattga tggtatagtt tttcaaagcc
     3121 ggcatgttgc tgaaatgcct ttttactgcg tcaaagactt tgggattttc tttctttatc
     3181 tcctcagtca atgggttctc aatgcgaata cctcggctca atcgaatcat catcggtgac
     3241 aaatgatcga gtagtccatt gaaaagctgg agatcctcta ctaatgaaaa atgaaattcc
     3301 tgagacaatc ttttcagaac tttcgctaat tcctttgata gttcctgatc attttgattt
     3361 tctttgtgat tcgacttagc catgagatgt aatgtcaaat aagcaatttc ttcttcggga
     3421 aacgatacac ctgcgattac ctcaatacgt tgaacgatcc gctgcgccac ttgatactct
     3481 actcgcttag aaacttcttg ttcgatgcct aattccttga tttcaaaccc ttctcggagc
     3541 cgtttgatcc ccaaagccaa gtgtaaagtc agattctgta tgataaaatc cgaaactttt
     3601 aacttagctt ctcttgtttc atcgagaatg ataatgacta gctcttcaaa actgatgtct
     3661 gggaaaaaac ggctttgccc taaatattct ttcaatgaat ttccataatg gtttccaaaa
     3721 aaagtatcca tgataaaatg ccgtcgattt ctttcttccc cttcgatcca tgtcccttga
     3781 ccatgcttag aaacgatttt tagtgaatat ggaatcaatc gctccttgat ttcttgcatg
     3841 tcctttgtca aactggaacg actgatataa agctcttctg ccaaatcatc cataaaaaga
     3901 atggcatctt ccaaaagcaa ttggttcaat atatactttt gtcgatcttc ggactcataa
     3961 aatccttgcg gttctctagc atatccaggt actatttctt gccgactcaa aaacaagtca
     4021 aatgtcagtc tttgtaaaat atgcagttgg tagccataac ctggtttagc aatgatctta
     4081 cctccgtttt tttctactaa ctctttgatc cgctgcaagt aattacggat tgtccgatca
     4141 gataataaca attcagaagc taaacgttga ctggtgacat actccttctt gtgatcaatc
     4201 aaatatctca acagcaattt ttccttctgt ttcattatca tccactccta ttccatcata
     4261 tgttctcttc gatcacctct gctaacttct ggatgcctgt agggattggg acatacgctt
     4321 ggaaagggat actaacaaca ggctttccaa cagattctcc taattttttg aatttatcca
     4381 aaaacatcgt cgtttgcggg ctgatcagaa ataaatcaaa atcactattc ttaatcatat
     4441 tatcgccttc tgtagctgaa actgcatcta cgagtacctc ttctcttttt tgcttaaaga
     4501 aatccgttgt ctttgaggcc atcatcgagg atgacattcc tgccgcacaa ataatcaatg
     4561 cttttttcat aaatcaatca ctccttttat ttttataaac caagcatatc ttgtaagcgt
     4621 tttctattca aaatatatat ttccgtagca ttgcggaaaa aatgtgtatc atatcttgaa
     4681 taataatttc tggcaccgtg atatactagt gatagaattt tttcaggagg ggatttagtg
     4741 agtatcacag tgaatatttt gtatacaagt acaaatggac aagccagaaa atttgcagaa
     4801 gaaatggtcg aaagaaacat tgtcaaagcg attcgtgcag aagaaggaaa cgagaaatat
     4861 gactattact ttccactaga agatccagaa tctcttttac tgatcgaccg ttggaaagat
     4921 gaagaagcaa tcgaaaagca tcacaaatcc gaaatgatgg ctcaaataaa cgaactccgt
     4981 aaaaagtata aattaaaaat gaaagtcgaa cgtttcactg atttaccgta aaaagataca
     5041 catggaatga tagcaattta tcatccatgt gtatcttcac tttttcctta ttttttattc
     5101 gatctcatct aatgattcaa accgtgtttt tctcttattt ttcggataag tatttcagtc
     5161 accccaatat acatgattcc taaacatacc ccaaggatat tctcaaaaat gcggatcgtt
     5221 actgctcctg gaaccccgaa taaactggca gtgattgcca aagcaccaaa agaattaaaa
     5281 actgttttat atctataggt agaacaaaca cctaaagcta gaccacctaa tataccgacc
     5341 cagttaaaag gaataaattg gctgatacca ataaataaaa cacatccaat caatgagcca
     5401 atgattcggt ctactgctcg ttctttgaaa ccaatcgaca tcaatccata gctagataca
     5461 atagaagaaa atgcaaaccc tgcccacatc aatcgctgaa aagggacata ctctcctgca
     5521 acgatcaaaa gactaatgcc aaatgcatag atgagcatcc aaattttttc ctgcgaatac
     5581 atgcctttca aaaaaatctc tttaaacaag cttttgtctt tgttcttttc tcgatgtttt
     5641 ctgtaaagga gaactgaaaa ccaacaaaaa aacaaaaata ataagctact tgtttgtgta
     5701 aagaaattct tgttcaacga atctttaggt aacgaataaa caataaagag atatgaaaag
     5761 ccgtacaaag aagcattccc cattttggga tcactagcgg ttgccattaa tatgctgctt
     5821 agtaaaataa aatgaatcaa catgcccata actgaccatt tgatcagctg gacatacggt
     5881 gctactaata gtgagaacat gacgattgct aaacctatga tcgaatggga aatcttataa
     5941 ccaaagtcaa caaaacgaat actcatcaac atacagaata aaaccacagc gtatggttta
     6001 tgcttcgatt caaataatgt tgtaattata atgataaaac aaatggcaaa caatacgatg
     6061 aataatgatc gtaaaaacaa tgaacaaata tacatgcact tttctttttt agaagctgct
     6121 tctcgtattt tttgttttaa aataaacgga tctagttgta gagactgata aaaatccacc
     6181 tgttgtcgtc ctttcttttt acttttattt tttacagtca tctatgacag gttgtaaata
     6241 ctatggaaca tcatatggcg ttgtcatttt cttgtcaact atttttccta ttcatgtttt
     6301 tataaaaaaa catatcgttt tcttgtatga ttaaatcacc acaaaataaa caactgagag
     6361 aacgatatgc tttatgaatg gttctatcaa aaaaattctg agatcaatag acaaaaattt
     6421 gatgattaca gaggtctctt acgaaacctt acagaagaaa aaaacattga tcgttgctgc
     6481 agttctcttc cctgctccat gtgtctgtag aaattgtggt tctgctgtgg tagatggaaa
     6541 taggaaatta atggtggtga aaaacaagaa gaaggaaacc attgtccact ttgaataata
     6601 taatcatatg ccaatggtta tacgcctaaa aaagcagcgc tatacctgta aaaactgtcg
     6661 aagccattgg aacgctcaaa gttattttat ccgtccaagg cactcgattt caaatcatgt
     6721 cagacataaa atcacttctt tactgactga aaaagtgtcg ttatttttta tttcgaaaag
     6781 ttgttaggta tctttgacaa ccgttattcg cacattgaaa gagttgagaa gctatttacc
     6841 aaagtcttcc aaaagaattc tgtcttgagt gttgagggtt gtgaatttcg ttctcacgtt
     6901 tccgttgagg ataaactgag ttttatttcc gcggatggcg agacagaaaa attaatcgag
     6961 aaaatttacc tacacaaaaa attcctcgat taacaaacta tttccttagc tgcactaatc
     7021 ctaaagaaat agaattctta gttacagaca tgaacgccac ctactttcag ctcaccaaac
     7081 atgttctacc aaatgcgaaa ttggtgattg atcgattact tggtttttcc acgcctttca
     7141 aggaggcgta acctttattc acgagttggt tgaagctttc cgagacaaaa gacccgactt
     7201 gtttttctcc ttattgacaa agagccatta aaactagaga aaaaattaca actgatcgct
     7261 gattatttgt agaaagaaaa tcaaagtatt acccaataaa cttgagtcat taggcaaaaa
     7321 acactgcgaa actcaagaat gattttcgca gtgtttttgt atatttttta ttaactagta
     7381 ttactacaaa ggtttatttt tcacttctca acgcagcaca aaattgtctt tacttattta
     7441 ctggttgggt tcaaccttct ctactccgat actaatattt gtttgtatac cccttctaaa
     7501 atcggtagtt tccattcaat tgatgggtac tagttgattt taaaaactat gcttattcaa
     7561 ttttttattt tagctcatat atttcgtttc tttctaaatt ttatactgat gaaaacaagt
     7621 agcacaccca atattgacaa tataaactgg ctaatgatgg tttctccagt ttttggaaga
     7681 tgcttatttc cactgcttgt tggattgttt cctcttatat ctgagctatc agcaactttt
     7741 ctattctctt ttgttaaaaa gatcgccact tcttgttctt tttcgatctt gaaagttact
     7801 ggtgtcgcat c
//
LOCUS       DAYYGS010000103         7789 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000103 DAYYGS010000000
VERSION     DAYYGS010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.105"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..277
                     /locus_tag="ACHA44_002448"
     CDS             110..277
                     /locus_tag="ACHA44_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HFM8905695.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(339..2150)
                     /locus_tag="ACHA44_002449"
     CDS             complement(339..2150)
                     /locus_tag="ACHA44_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HFM8905696.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2147..2512)
                     /locus_tag="ACHA44_002450"
     CDS             complement(2147..2512)
                     /locus_tag="ACHA44_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFM8905697.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2675..3070)
                     /locus_tag="ACHA44_002451"
     CDS             complement(2675..3070)
                     /locus_tag="ACHA44_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFM8905698.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3088..4050)
                     /locus_tag="ACHA44_002452"
     CDS             complement(3088..4050)
                     /locus_tag="ACHA44_002452"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HFM8905699.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4050..4262)
                     /locus_tag="ACHA44_002453"
     CDS             complement(4050..4262)
                     /locus_tag="ACHA44_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HFM8905700.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4283..4981)
                     /locus_tag="ACHA44_002454"
     CDS             complement(4283..4981)
                     /locus_tag="ACHA44_002454"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFM8905701.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5001..5543)
                     /locus_tag="ACHA44_002455"
     CDS             complement(5001..5543)
                     /locus_tag="ACHA44_002455"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFM8905702.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5675..6679)
                     /locus_tag="ACHA44_002456"
     CDS             complement(5675..6679)
                     /locus_tag="ACHA44_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8905703.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6676..7665)
                     /locus_tag="ACHA44_002457"
     CDS             complement(6676..7665)
                     /locus_tag="ACHA44_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFM8905704.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7662..7766)
                     /locus_tag="ACHA44_002458"
                     /pseudo
     CDS             complement(7662..7766)
                     /locus_tag="ACHA44_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT         2447 a         1743 c         1301 g         2298 t
ORIGIN      
        1 aaacattttc aaggattttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt
       61 ctcttttgtt ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt
      121 tgtatctgga atattagtag gaagtctggc aacagccgct gccgttgctg gtttggtcgc
      181 ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa
      241 ccgtaaaaaa gcaatgcgca aacgcgtttc tcgttaataa ttatttaaaa acacgcatga
      301 agacttgtga aagaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt
      361 tgtgctattt tatctgcctg tctcagattc catggatctg tataaggtct agctactgct
      421 gcagttagcc aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt
      481 tcccctgaag catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga
      541 taacttcctg tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt
      601 tttatgacag gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa
      661 tagcgactgc tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga
      721 atatcaaatg gcggttcgaa caaatgaaaa atacccgcat ctatcagtgc tgctaattct
      781 ttcgtccgaa tgactggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc
      841 catagtattt ctttgtattc ctttgcagaa aaaacgtgcc attcaatagc ctgccggatt
      901 ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct
      961 ttttccgctt cttttatctg cccttcaata aatgctcggc tagcttctgc aaaggactca
     1021 tcctgatagg taagaggaat ttctaaatac gaccaatccc atctataagg gattagtgct
     1081 ggatgctttc cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc
     1141 tttggaatcg gcaaatgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct
     1201 tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc
     1261 aggttttctt ttgttagtaa ccgtggccat gccattgatc cgccttgctt ttggtttctt
     1321 cctcgagggt aataagggag cccccttctt gatccactgt atacaatcgg ttccttgcct
     1381 gatggatgat agacaagttt tcctttttcg ttctcgaagc gcccttctct tgatactgta
     1441 aacaatccga cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta
     1501 ggcggtatct tcgaaagatc gacatctgca ggattagaag gaagctgata cagtaaatca
     1561 tttttctttg cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga
     1621 ccgcttgcta aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa
     1681 ccatcttttc ttttgctgac tttttttact aaagaataaa caaactgaat agaagctgga
     1741 aattcttctt gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta
     1801 gattgctgat ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt
     1861 accgtctgtt cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc
     1921 tgtgttacca atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa
     1981 acagaattca ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca
     2041 ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt
     2101 tctgctatac ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct
     2161 ccatttcgat aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat
     2221 aaacaaaagt aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct
     2281 tcttttcttc ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt
     2341 ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca
     2401 gatctaactg cgctttttct tttgaaattc ctcgtggcca gaacctatcc acgagtattc
     2461 tatagccatc ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt
     2521 ccttctttct gccttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa
     2581 tgaacggcac aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga
     2641 ttcggttttt aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc
     2701 cagaccatcc gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta
     2761 atttatttgc tgtacggatg ctcaagcttt tcttttgatc gtaaatatag acaggctggt
     2821 cttttcgaat agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta
     2881 aaatatgccc agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca
     2941 ttccttctcg aaattcttct tctgtcaaag tcgttgctga acgcttggcc attactcgta
     3001 gatagacttc ccaaaagatg attgcaagca caatcaatag caaaatgata ttaattaccc
     3061 ataaaaccat gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc
     3121 aatgacacca gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg
     3181 cgggaaagta aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc
     3241 cgatactcct ccacctaaca caatacttga cggattcaat gtattcccca agttggcgca
     3301 agctaaacct aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc
     3361 ttgagccatt tcaaagatat ctttacttgt tacttcttca ccgttgtcta acattgcttt
     3421 cagttttgaa tctcctgcgt attcttctgc caagtaacga cctagacgta ccacaccagt
     3481 agcgctagac acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg
     3541 atccacagtg atatggccga tctctccgcc tgctccgctc acgccgtgaa tcaaatttcc
     3601 gccagcaacg ataccaccgc caacacctgt tcctagagta acgaatacaa cgtctggatc
     3661 gttttctcca gcacctttcc atcgttcacc taaagcagca acattcgcat cattatcgat
     3721 agcgaatttg attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca
     3781 gtttaagtta tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc
     3841 catgccgatt ccgataaagt cttcggcttt catattatat aaattcaaac gatggttgat
     3901 tgattcaatg atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct
     3961 ccatttttgt tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc
     4021 taaatcaatc ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca
     4081 attcggtgct ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa
     4141 cggctatcat acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca
     4201 cccacataga taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc
     4261 atctttaagc acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa
     4321 tacatattac aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat
     4381 agcttgtctg ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc
     4441 caccgacatg tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca
     4501 caataaataa aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa
     4561 accctaagat aaacaacgca ccgaacaaac cgaatatcga agtactcgca ccaccagaca
     4621 gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat
     4681 aaatcaagaa aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca
     4741 gtatcagtga gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta
     4801 aacgccacca ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga
     4861 tctcaagtcg cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag
     4921 cataggtcca taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca
     4981 tgaaaacctc ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc
     5041 tccggttgcc attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga
     5101 aaattattta aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta
     5161 ttgtagataa tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct
     5221 gattgtggct caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca
     5281 taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt
     5341 tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct
     5401 atcacttttg ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct
     5461 ttttcctttt tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt
     5521 aatcttactt tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga
     5581 aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac
     5641 ctagaaatga ttggccttcc ttttttaata aatttcaact gttcagcgcg tgtttttgtc
     5701 tgataaaaag tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag
     5761 gtacttcccc acttggtaga atcactctag caaccatatc tgcactaagc agaaaaatgg
     5821 aaccgaacaa accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg
     5881 cagcatgagg agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc
     5941 ttgccaaagc aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta
     6001 atccttgcgc gcgttcttga ccaaatgata gcaatccaat cctctgtgca taaagtcctc
     6061 ctagcggaat taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac
     6121 cccagattga tcccgctaac catgcattca caaatccata attttcctta gataattgga
     6181 tcgtgcatag caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga
     6241 ttcgattcat acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag
     6301 ctaaaacacc acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa
     6361 agaaacctaa ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag
     6421 aatctgccaa ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag
     6481 ctcctgctaa taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca
     6541 aagaagatga ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg
     6601 taccaatcat taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg
     6661 tccaatatat ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc
     6721 aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt
     6781 aaaatccgcc aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg
     6841 atagtccgtt ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac
     6901 aggtccgact aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa
     6961 ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa
     7021 agatattgct tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa
     7081 tactgctaat tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc
     7141 ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat
     7201 agctacgcca gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa
     7261 accaaatacg agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa
     7321 atcagaagct ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt
     7381 gattcctagt aagcctgaat ctgctaaagg atttcttgtc actccttgca tcaatgcacc
     7441 gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt
     7501 tcgtatgatt tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc
     7561 tgcttttccc acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac
     7621 aaaacagacc aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc
     7681 tcttttggca acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca
     7741 atcgttgcat caatctcaaa tattttggct cttcgtcaaa ttgtgttgg
//
LOCUS       DAYYGS010000104         7771 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000104 DAYYGS010000000
VERSION     DAYYGS010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.106"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..107
                     /locus_tag="ACHA44_002459"
     CDS             <1..107
                     /locus_tag="ACHA44_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFM8905705.1"
                     /translation="NESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(176..904)
                     /locus_tag="ACHA44_002460"
     CDS             complement(176..904)
                     /locus_tag="ACHA44_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HFM8905706.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(917..2197)
                     /locus_tag="ACHA44_002461"
     CDS             complement(917..2197)
                     /locus_tag="ACHA44_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HFM8905707.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2338..2940)
                     /locus_tag="ACHA44_002462"
     CDS             complement(2338..2940)
                     /locus_tag="ACHA44_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HFM8905708.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3185..3391
                     /locus_tag="ACHA44_002463"
     CDS             3185..3391
                     /locus_tag="ACHA44_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905709.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3703..4422
                     /locus_tag="ACHA44_002464"
     CDS             3703..4422
                     /locus_tag="ACHA44_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HFM8905710.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(4503..5186)
                     /locus_tag="ACHA44_002465"
     CDS             complement(4503..5186)
                     /locus_tag="ACHA44_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HFM8905711.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5405..7591
                     /locus_tag="ACHA44_002466"
     CDS             5405..7591
                     /locus_tag="ACHA44_002466"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFM8905712.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2784 a         1309 c         1466 g         2212 t
ORIGIN      
        1 gaaatgaaag tcaagaagta ttgctgaaag aaatggagaa ttggccaaca ttttatccag
       61 atacaatgga tggacgtgaa attgcacagg aaatgctttt gcactgatag gcaaaaaaga
      121 accgaagggt ttggatcggc tggtcattta gtcggtccaa actattcggt tctttttatg
      181 aaatcaattt ctttaatcca cggatagagg ctgggatggt ctcgccgttt gccaacgtga
      241 cttctctggt ttttttatta atcgaccgca cattttgtgt attgataaca taggatttat
      301 gacaacggat aaaattccga tgcattttct cgatttcttt gatcttctca tagaattgta
      361 attgggcatg agtcaaatgg acttccagtt tatgtggcgc tcctagcgaa gtagaaaaat
      421 attggatgtc tttgatcggt aaaaatttga tctcatcatt aaactctatt ttgaagatat
      481 ctttttggat ttgggacatt tgaaaatctt cgaaaatttt gctcaggata tgctcgacat
      541 tttcttttaa atcataaaga tcttctttga cgatataatc agtcggttcg atgttgcttt
      601 ttaagatttt catcgacatt tccgtatggg aagtcacata gattattttt cctaaaggat
      661 ctaattcacg gatcaattta ccgatcgcta caccatccat ttcactgtct tctagttcga
      721 tatctaaaaa gtaaatacca aaagaagaag ttctgatctt ggctgcattg agaagttctt
      781 ccggatcaga ggtagaaata tggacataac tgtctaagtt ttcaatcatg attctatttt
      841 tgatcaccgt tgataaaact tctaattgtt ttggatcgtc ttcacaaata taaatgggaa
      901 tcatatcatt actcctttat tttatttgta tataaatcgt ttgagaaaaa cgctggtttt
      961 caattgatgt ttcaaggaaa agataaggat tggtctttgt caattcatcc aagataaaca
     1021 agccaaggcc ttcgtttttt ggatttgttt ttgttgagaa ccctttttgt ttcaactggt
     1081 ttaacggtaa tgtatttgtt gttgtattga tcacacgaat catcacggaa tgattcttct
     1141 tgagtatggt caattcgatt tttttcttct ttgcagcact agctccttca atcgcattgt
     1201 ccaagataat accaagcata cgtacaaggt ttaccgtatg tttcgtatcc agttcgattg
     1261 gctcaggaaa atgaagctgg acgatcaacc ctttttcctg tgccatcatg agttttaatg
     1321 acagtaaact acggatctca ggaactttca tattatccaa catacttaga ttggccggaa
     1381 aaaggttcat catctggcgt gtaggtaata tcgtttgatc ataataagac cgtaatcctt
     1441 ctaagtcgcc ttctgaaata tagctgtgaa gagaaaaaag cagattttgg taatcatgtt
     1501 tgaattctct caagtcttga tagttttttt ctagatcttt gatataatcc tgttgcatcc
     1561 tgacttgttg ctctaataac tgatcagcta atttcttatt agagattttc caataataaa
     1621 ccaagatgat caggccaaca cacagatata gaatgaaaaa actcgtattc aaagctaaga
     1681 catcaggtgt ttcaccagaa aaacgggtat aaaaaataat gatatagtat gtaaataata
     1741 tcaatgtccc gatcgtaccg atcatacggc ggtccacagt gtgaaaccaa gtctctaacg
     1801 atttttttaa acaaaaagct aataaagcag ataaaaaagc ggataagaca atgtgaaaat
     1861 actgcaatga atctcctggt tcaataaccg aagatttcaa aacaaaaaaa tcgccgatgc
     1921 ttgttaagtg atctcctaaa agcgacgtca ataacccaaa aggaagcgcg taaaccaatt
     1981 gttgacgcga agtcgaaaag aagaaaaaag taagcatcag cattcctata gcaattagag
     2041 cagtaaaaat ccctaaaatc gaatagaccc atccaatcaa aatacaagaa atcagaatga
     2101 ctcccgcaaa cagatatttg tatcggatgt gatggtcaat caataagata caaattgtgt
     2161 aaatcaaata ttgcaacgct atggaccata aaaacataaa gtacagctcc cattgatttt
     2221 taccaaatta tagcacaatt ggcgtattat ttttttaatt tttcagggat tttgaattca
     2281 gaactcatac caaagcagca gccagataca aaaaagtcag ataagttagt taattgttta
     2341 atgattgttt ttttcatgaa agattcctcc aaaattcatc aagtagtttg taataggaag
     2401 caacgtagct atttctaaaa caatcccaag tagcaaatag gtagaaaagt aagaagctga
     2461 gaaaaataac gtaatgagag aaaaaattag aaacatgctg atcgccttct ttaataatgg
     2521 gggacgtttc tcgggttcaa tgggattgat tgctgtacct cttgatccat agcgaagcag
     2581 taacaaaaag cagctgatta acaaaaagac catcattggg aaggagatag tcaattgcag
     2641 atgtttcatc accaagggaa cgccccataa ataaagtagt gtccacagca gacaaacgaa
     2701 ttcagagtcg gaatgcaatc cataagcata tctgcggatg cagatataag aaagaaaggt
     2761 gattcctgtt tccgcccatg agcctgtcac cagagaaaag actagaacga ccgctagttt
     2821 tccaagatta ttcaataaaa cttctaagac aaattttact tggacataca ccatatcttc
     2881 ttccgatttg tctccgctga ataaggcttc taataatttg ttcacaactt tatctttcat
     2941 ctcatacgta cctccaccac aaattttatg ctaaaaaaaa taaaaaacgt cttttgtttc
     3001 ataaaaagtg gttttgacat caaaaccgtc atgatctcct aaaaaaatct tcgttttcca
     3061 agaaccttac caaaaaaagt gacagttttg atacgaaaag tactttttat gacaagtttg
     3121 tgctttttcg agtgatttat gttaaaaaat tactgacctt ataaaaggtg atggagggat
     3181 tcttatggaa aatgaacaat tgtttgaagt cggcgaaaca tatgcattta aatctgattt
     3241 atttgaatca ccaataaaag gaaaaataga aaaaatctat gaaaatagtg tgatgatacg
     3301 tgtcatgaaa tgcgcagtag aagacaatga taccgctcat aatctgcact ggcagttagt
     3361 tgtacgcaaa tcaagtacaa tagaagaata aaaacagata aaatgttgag agcataaaga
     3421 ggttgtgagt ctcccgttta gctctgagtt acaaggaata atgttaaaaa acagcttttc
     3481 atgttttatg aatattgcct tgtgaccgag gtgttggcag gagaatactg tttatttgaa
     3541 aaaagggctg cgacaagact tttgtcacag cctttatttt catgaatagg agttactttt
     3601 ggctactttt agagaagtag cttttaagga gcagataaag tcaaggcttt tatttctata
     3661 aatgaagtag taaaatgaag aacagatttt attacaggaa ggatgccgat aattgtgatc
     3721 attgttgcag gaacgatcgg tgcagggaaa agcacactaa ctgaaatgct tgcccaagat
     3781 ttagaaacaa aaccttttta tgagaacgtg gaagataatg aagtattgcc gctattttat
     3841 tctaatccag aaaagtatac ttttttatta cagattttct ttttgaacaa acgctttttg
     3901 gcaatcaaag atgctttcag ccatgatgac aacgtactag accgctccat ctacgaagat
     3961 agtatgctgt ttcatttgaa tgcagattta gggcgtgttt ctgaagtaga agtaaagcag
     4021 tatgaaggtc ttttagaaac gatgctgaag gagttagaag aaatttcgcc acaaaagaaa
     4081 ccagatctac tggtttatat tcgtgtatcc tttgaaacta tgttagcgag aatcaaaaaa
     4141 agaggccggg aatatgaaca gctagaacaa gatcctgaac tctattccta ctataaagaa
     4201 ctcaatcgac gatacgaaga gtggtatgaa cagtttgata tatgtcccaa aatagtgatc
     4261 gatggggaca aatatgattt cgtagccgat ccggcatgtg gagaacaaat cgtacaagaa
     4321 atcaagatgc gggcaaagaa aatgacagaa gaggcagatg cccagtcgat ccgctacact
     4381 acaaataacc cgaaagcaaa gggaatcttt acaactaaat aatataaaaa ctaagcaaat
     4441 tgctcactcg cagatatttc tctgtgagca gcatcttgct tagttttttt gattcattga
     4501 gatcaaatag tgaaccgatt cgtatgataa aattgccata aatgaaatgt ctgagtgagg
     4561 gccatgatca aaaataaaat ggaaacgatc aaaatcgaat gggcgccaaa cagatgatga
     4621 cagaaaacaa ggacaatagc tcctgcgata aaggcaagaa cgacacttag atagtggaaa
     4681 aatcgatcaa aatctgcttt ttctttagaa atacaataat catataagtt cgttgctacc
     4741 ccttttaaat ttccagttgt aaataaatta gtgtaggcac gcccgttgat tttatcaaaa
     4801 gctacccact ggatcgatgc agtaaaagca atcaaaatcg ttactaatgc gggatgctca
     4861 tggacgggtg tcaaactaac aaagaaaaaa atcactgctt cataatatag aacaaacaag
     4921 cgccagaaat gtttttcttt ttttctaaaa taatcaatta gatactttgt tagaagaatc
     4981 cctaagaaaa agaaaagggt agacaatact tttttaccaa cacttagcca ttctccgtca
     5041 aatgcttgga tgatcgatag aatgatattg ccagtttgag cagaggcaaa agcaccatat
     5101 tggatataag tataactgtc catggctcct ccgataaatg tcagcaaaag tcctattgta
     5161 cgcccctcgt gaaaggaagt ctgcattttt ttcatcacct tttatgaata tttttgtctt
     5221 ccttatctta gcataaacga ttgaaaaaaa aggaaaaatt ttcagaaaac taaaatcata
     5281 tcgcttccca ttttcatttt tagaaaggtc tgttagtata aagaaaaaat agacaacgaa
     5341 actttaaata accaacttga aataaacaaa ttcaaagaag aaagatggga aggaaatcaa
     5401 tcagttgaat gaaaaagaaa ttctccgatt acaaaaagaa ttgaaacgct gggagaacaa
     5461 gtataaaaag cttcaacaag tctttcggct acaagaagaa atcattgcta cctatgaagc
     5521 ggcttatggc gaagcacccg gacttattga ggaagaagta caagtaaaag aaagtgaagg
     5581 aaacagctct ttctttaaaa tgatggagaa acgttcctat aatcgcttca atcaagaaca
     5641 aaaagcagct atcacatatg atatgaagaa acatcttcga atcatagcag gagctgggag
     5701 cggcaaaacg caaacaatct gtgcaaaagc tgtttatctg atgacgcaaa aacaagtaga
     5761 cgaagaacgc atactaatga tcacttttac aaggaacgct gcaaatgaac taaaaaaacg
     5821 agtagataat ttcagccaga gaaaaaccgc tgtccatatc ggaacattcc atagtatctt
     5881 ttttaggata tacaatgaaa tttgtcgaaa atttcctgaa gtagcaatgc aaggaataca
     5941 aggagacttt tcaaaggata ctgcacaaaa agtgaatgct gttttgcaac agctgatacg
     6001 taaatataat ctttatatat ttgatcaata tggagaaaaa acgattgctt ctcggttaga
     6061 ctattggcag aatatgaacc tttcagtaga agaaatggtt cagttagtta aagaaaaata
     6121 tgattccatt gataagaatg cacgacagcc gatatcagaa cgactgtatg gattgctcac
     6181 ggaactccaa gaacaaaaaa gaagccagca attgctagag ttcaatgatg tactccaaaa
     6241 tctgaaaagc gctttggaga atgaggagat ccagcattat atcggccaaa aatatgatta
     6301 tctattcatt gatgaatttc aagatactaa tccacttcaa tgggaaatcg tgaaacggat
     6361 gacaaaggaa aacaggatca agttgattgt tgttggagat gatgatcaga gtatctatgg
     6421 attcagagga tcagagccag catacatcaa gaactttgaa aaagaatatc ctaccaaaac
     6481 tcttttctta ttaactaatt atcgttctcg ggcagctatt gtccaaggag caaatagatt
     6541 gatcagctat aataaaaatg atcggatacc aaaggcgatg attccagcac aaaaagaagc
     6601 aggtgttatg gaagcctatt tgttttctga tactaaagca gaaagtcagt ggctaatcaa
     6661 acaaattcag acatttattc aaaaaaatgg gaaatataag gaatcaatca tattataccg
     6721 atcagcttca caaacgcagc agcttgtcca atctctgctg aaaacgaata ttccttttgt
     6781 actggaagca gatagtccat atgaaggtat ctttggtatc aagtctttcc aacatttcta
     6841 tcaaaaaatt gttcattggc aaacagcact gaatctgcaa aagaaaaatc aggcctacca
     6901 gcaattgctc cggcagctga tggcagactg ctacctgaaa aaaagtgaat gcgatcaata
     6961 cttttctcct aaatatcaaa atatagccgt tgcagattat attttgtcga taagccccaa
     7021 tttaaaatcg aaatctgcag aaatccgaga gtttgaggaa gcgttgcgta aaacagacca
     7081 gcatcaagaa atctattcgc ttgttcaaag ctatcttcat ctgccacgta tagctaagga
     7141 aatcgaccag agtgatcaag aatggatacg agaagaagta aagcagcatg ctactttcag
     7201 aagactccaa gccttatcgc aagaaaccca agcgacagct aaagaattaa aaacccgctt
     7261 gattgcttac agacaaggaa aacttgatgc actatgtatc cagagcattc acaaaagtaa
     7321 aggtcttagt taccgaaatg tctttttgat tggttgtaac gaaggaagtt tgccttataa
     7381 cggagcgaca gaaaagaaag taatcgactt taatgaaata aaagcagaac cagtaacgac
     7441 gatagaagaa gaacgaagac tattttatgt tgcgatgact agagcaagaa atcgtctgta
     7501 tctatgtgta cctcaaatga aaaattcaag aaaattgaaa gtatcgagat ttatcaaaga
     7561 aaccgggagt cgtgtgaaaa aaggaaagtg atagaagaga gggtgtgaca gaagtggtca
     7621 gtttcaagaa agaagaagga atgtacgaaa attgcttctc aaatttttgt gaaatttcag
     7681 cttattttcg aagaagctat ttctggaaca cagtttattc ggaaaaggga ggtgtgacaa
     7741 gaattttgtc acacctccct tatttatttt g
//
LOCUS       DAYYGS010000105         7765 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000105 DAYYGS010000000
VERSION     DAYYGS010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.107"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..151)
                     /locus_tag="ACHA44_002467"
     tRNA            complement(79..151)
                     /locus_tag="ACHA44_002467"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="ACHA44_002468"
     CDS             532..1101
                     /locus_tag="ACHA44_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HFM8905713.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="ACHA44_002469"
     CDS             1088..1948
                     /locus_tag="ACHA44_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HFM8905714.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /gene="yycF"
                     /locus_tag="ACHA44_002470"
     CDS             2138..2842
                     /gene="yycF"
                     /locus_tag="ACHA44_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HFM8905715.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="ACHA44_002471"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="ACHA44_002471"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HFM8905716.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /gene="yycH"
                     /locus_tag="ACHA44_002472"
     CDS             4679..5995
                     /gene="yycH"
                     /locus_tag="ACHA44_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system activity regulator YycH"
                     /protein_id="HFM8905717.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="ACHA44_002473"
     CDS             5996..6865
                     /locus_tag="ACHA44_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HFM8905718.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="ACHA44_002474"
     CDS             6920..7729
                     /locus_tag="ACHA44_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFM8905719.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
BASE COUNT         2650 a         1316 c         1696 g         2103 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaagg tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagat
//
LOCUS       DAYYGS010000106         7197 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000106 DAYYGS010000000
VERSION     DAYYGS010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.109"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(3..689)
                     /gene="gpmA"
                     /locus_tag="ACHA44_002475"
     CDS             complement(3..689)
                     /gene="gpmA"
                     /locus_tag="ACHA44_002475"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8905720.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(861..1442)
                     /locus_tag="ACHA44_002476"
     CDS             complement(861..1442)
                     /locus_tag="ACHA44_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905721.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1575..2258)
                     /gene="rpiA"
                     /locus_tag="ACHA44_002477"
     CDS             complement(1575..2258)
                     /gene="rpiA"
                     /locus_tag="ACHA44_002477"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFM8905722.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2577..2990
                     /gene="rpsL"
                     /locus_tag="ACHA44_002478"
     CDS             2577..2990
                     /gene="rpsL"
                     /locus_tag="ACHA44_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFM8905723.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3074..3544
                     /gene="rpsG"
                     /locus_tag="ACHA44_002479"
     CDS             3074..3544
                     /gene="rpsG"
                     /locus_tag="ACHA44_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFM8905724.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3638..5725
                     /gene="fusA"
                     /locus_tag="ACHA44_002480"
     CDS             3638..5725
                     /gene="fusA"
                     /locus_tag="ACHA44_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFM8905725.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5897..7084
                     /gene="tuf"
                     /locus_tag="ACHA44_002481"
     CDS             5897..7084
                     /gene="tuf"
                     /locus_tag="ACHA44_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFM8905726.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         2168 a         1412 c         1464 g         2153 t
ORIGIN      
        1 ttttataggt agtatttttt agcaactgtt aagtcatcat ttaattcata aacaagtggt
       61 tgacctgttg gaatttcaag atccatgatg tcttcgtcag agataccttc gatatgttta
      121 gctagggcgc gtaatgagtt accatgtgca gctactaaaa cagttttgtt gtctagtaat
      181 gctggagcaa tttcatcttg ccagaatggc aaagcgcgtt ctaatgtcac tttcaagttt
      241 tctccaccag gaatatcgcg tttgtctaac attgcgtaac gacggtcatt tgctgcagaa
      301 ccttcgtcag tttcttccat taatggagga agagtatcgt aagaacgacg ccagatgtgc
      361 acttgttcgt cgccgtattt ttcagctgtt tcttttttgt tcaaaccttg taatttacca
      421 tagtgacgtt cgtttaagcg ccatgatttg atttgtggta cccaaagttg atctgaatat
      481 tctaagatca agttacatgt tttgattgca cgagttaaaa cagaagtgta agcaacatca
      541 aattcgattc cagcttcttt gattttgcgt ccgccttctt ttgcttcttc gattccttct
      601 ggtgctagat ttacatcagc ccaaccagta aattggttca atgcattcca ttcactaaga
      661 ccgtgacgag aaaaaactaa ttttggcatg tttagctctc cttttaagat aggtttatat
      721 ttctgaaatt ttcagttctg ttttattgta caattttttg gaagagattt ccatcgaaaa
      781 attagataag attctatgag aaaacaagta gagaatataa aaaaacgaac agtgcccacg
      841 caactgttcg tttttaggta ttagatgcat aattgctcga tttctttttc aggtaaattg
      901 atatatttat aacatgttcc aactgaaaca ccgcattttg aagaaataaa ttggatcgtt
      961 tctttttttt cataatagag acgtcggatt tttttgatcg tttttgcatc gatttttggt
     1021 cttccgccga tttttccttt tttacgagct tcatctaatc caactaatgt acgttctttg
     1081 atcaatgcgc actccatgtt tgctaggttt tccattaatt ggaaatagat ttttcccatt
     1141 tcgcgcgtat cgattccttc atcaagacta atcaagtgca gcccgcgatc tttgaataat
     1201 tgcgtgagtt ctgttaattg ccgcgtagat ttgcctaatc gatgaagttg gcaaatgact
     1261 agtgcatcgc cttgttgcat gtcagcgatc actgactcca aatgaacagc agtagaatca
     1321 ttttgttcca cttcatatgt ctcatggaaa atttcttgac aaccaaagtc agataattga
     1381 tcaacctgag ttgttaaatc aacgtcattt atggttgtgc gtgcataacc aataattttc
     1441 atggtgattc ctttctagca gtgattttct aactggatat aacagtaaaa atgtagcata
     1501 aagtacgtca aaaagatagt tttttcgcaa attttccgct gtcttttttt attggatgat
     1561 caagcagttt ttctttattt acgacggatt gcgtcaagaa tctcaacgcc tttttctgag
     1621 ccgacaacta ctttagaaac catgtttagg aaaagcccat gttctactac cccaacaagt
     1681 tcatctaaat aggtagctaa gctttctggc tgttcgatga cttctagatg cagatcaatg
     1741 atgtaatgac caccatctgt ggttaagact tctccgtttt cgtctgtacg caagacgggt
     1801 tggaatcttt tttcttcaaa aatatgaagt agctgttggg agccataagg gatcacttca
     1861 actgggagtg ggaacttccc aagtttatcg actaatttag aagaatcaac gatccaaatc
     1921 gtttcttttg agtacgtagc gactattttc tcgaaaagca aagcagcgcc gccgcctttg
     1981 atcccttgaa aatcagcact gatttcatca gcaccgtcta ttgtcaaatc gactacgggg
     2041 acttcatcaa tactctttaa cggtattcct aatttttccg cttgttcttt tgtcgcattt
     2101 gaagtagtaa ctcctgtgat acgtaaacct tcttcacgca tacggcgacc aatctcttct
     2161 accatataat aggcggttga accggtcccg agacctacga tcatcccgtc ctttacgaat
     2221 ttcgcggctt caatgccagc taattgtttt aaattcatct ttgcgtccac ctttttttag
     2281 tttgagcatc gtgctttcct cactatttta gcgtgattta cggtaaaaag ataccattct
     2341 tttttattcc aataaagctt tttcaaaaaa aattgcatct cttgttgaca tgttttttca
     2401 ttcgtgatat tctaacaaag gtgctttatc tacaggtttc tgtgaagaaa cgtgctgact
     2461 gtttatcaaa gcatatttga taaggatgca gacattgcat ttatttttta tcacaaaaaa
     2521 agaaccacct ggatgtgtgg aactagacgc aaatcgtggg aaggaggaaa caaggaatgc
     2581 ctacaattaa ccaattagta cgtaaacctc gtaaatcaaa ggtggaaaaa tctaattcac
     2641 ctgctttgaa caaaggatat aacagcttta agaaaactca aactaacgta aactcacctc
     2701 aaaaacgtgg ggtagcaact cgtgtgggta caatgacacc taaaaaaccg aactcagctt
     2761 tacgtaaata tgcacgtgtt cgtttgtcaa acttaattga agttactgct tatatcccag
     2821 ggattggtca taacttacaa gaacacagtg ttgtattatt acgcggtggt cgtgtaaaag
     2881 acttaccagg ggtacgttac catatcgttc gcggtgcttt agatactgct ggtgttaacg
     2941 accgtaaaca aagccgttct aaatacggaa ctaaaaaacc taaagcttaa ttttaattat
     3001 taaacaaatg aaacttgctt ctactatata gaagtaatta ctatgaacaa tatttcggaa
     3061 ggaggagtta cggatgccac gtaaaggtcc tgttgcaaaa cgtgatgttt taccagatcc
     3121 tatttataac tcaaaattag taactcgctt gatcaaccgt gtaatggttg acggaaaacg
     3181 cggtattgca gctaacatta tctataatgc ctttgacttg atcaaagaat ctacaggtaa
     3241 cgatccatta gaagtgtttg aacaagcaat gaaaaacgtt caacctgttc ttgaagttaa
     3301 agcacgtcgt gttgggggtt caaactacca agtaccagtt gaagttcgcc ctgaacgccg
     3361 cgttactcta ggtcttcgtt gggtagtaaa ctacgctcgc ttgcgcggtg aacacacaat
     3421 ggaagaacgc ttagcaaaag aaatcatgga tgctgcaaac aacacaggtg cttctgttaa
     3481 aaaacgtgaa gacactcata aaatggctga tgccaaccgt gcgtttgctc actatcgctg
     3541 gtaagattct ctctgtttgg tcggctttta taagtcgacc tcagatagtg tccttatttt
     3601 tagcaactag atagtaaaga gaggagaaac aaggagaatg accagagaat tttctttaca
     3661 aaacactcgt aatatcggta tcatggccca catcgatgct ggtaaaacga ctgctactga
     3721 acgtatcttg tactatactg gtaaaatcca taaaattggt gaaactcatg aaggagcttc
     3781 acaaatggac tggatggaac aagaacaaga acgtggtatt acgatcactt ctgctgctac
     3841 tacagcacaa tggaagggtc accgaatcaa catcatcgac acaccaggac acgtagactt
     3901 cacagttgaa gttgaacgtt cattgcgtgt acttgatggt gctgtaactg ttcttgatgc
     3961 acaatcaggt gttgaacctc aaactgaaac agtttggcgt caagcaacaa cttatggtgt
     4021 acctcgtatc gtatttgcaa acaaaatgga caagatcggt gctgacttct tatattcagt
     4081 atcaacttta catgaccgtt tgcaagcaaa cgctcaccca atccaattac caattggtgc
     4141 ggaagacgac ttcacaggaa ttatcgacct tgtaacaatg aaggctgaaa tgtatacaaa
     4201 cgatcttgga acagagatcg aagaaacaga aattcctgaa gaataccgcg aattagctga
     4261 agaatggcgc gaaaaattgg ttgaagctgt agctgaaact gatgaagaac ttactttgaa
     4321 atacttagaa ggtgaagaaa tcactgaagc tgagttaaaa gaaggtatcc gccgcgcaac
     4381 agttaacgta gaattctacc cagtactatg tggttcagca tttaagaaca aaggggttca
     4441 attactattg gacgctgttc ttgattactt gccatcaccg ttagatatcc cagctatcaa
     4501 agggattgac cctaaaactg atgaagaagt agaacgtcct gcagacgaca gcgctccttt
     4561 ctcagcttta gcatttaaag ttatgactga cccattcgta ggtcgtttaa ctttcttccg
     4621 tgtgtactct ggtgttttac aatcaggttc atacgtacaa aacgcaacaa aaggcaaacg
     4681 tgaacgtgtt ggtcgtatcc tacagatgca tgccaactct cgttcagaaa tctctgaagt
     4741 ttacgctgga gatatcgctg ctgctgttgg tttgaaagac acaactactg gggacacact
     4801 atgtgatgaa aagagtctag ttattcttga atccatggaa ttcccagaac cagttatcca
     4861 agtcgctatt gaacctaaat caaaagctga ccaagataaa atgggtgtgg ctttacaaaa
     4921 actatctgaa gaagatccta ctttccgtgc tgaaacaaac gtggaaacag gcgaaacaat
     4981 catcgctggt atgggtgaac ttcacttaga tatcatcgtt gaccgtatgc gtcgtgaatt
     5041 caaagttgaa gctaacgtag gtgcgcctca agtatcttac cgtgaaactt tccgtgctgg
     5101 cactcaagcc gaaggtaagt ttgtacgtca gtctggtggt aaaggtcaat acggtcacgt
     5161 atggattgaa tttacaccaa atgaagaagg tgctggtttc gaatttgaaa atgcgatcgt
     5221 cggtggggtt gttcctcgtg aatacattcc agctgttgaa actggtttga aagatgctat
     5281 ggaaaatggt gtcttagctg gctatccatt agtagatatc aaagctaaac tttacgatgg
     5341 ttcataccat gatgtcgatt caaatgaaac tgccttccgt gttgctgctt ctatggcatt
     5401 acgtgcagca gctaagaaag ctaatccagt aatccttgaa ccaatcatgg ctgtagaagt
     5461 tgttatccct gaagattatt taggtgatgt tatgggtcac gtaactgctc gtcgtggacg
     5521 tgttgaaggt atggaagctc gcgctggcgg ccaacaagtt gttcgtgcga tggttccact
     5581 tgctgaaatg tttggttatg ctacaactct tcgttctgct actcaaggtc gtggtacatt
     5641 tacaatgaca tttgaccact acgaagatgt accaaaatca gtacaagaag aaatcattaa
     5701 gaaaaacggc ggaaaagctg aataatttct tgaaaccgga tagtcctgca aagagattat
     5761 ccaaagagac tatattttta atatagtttc gtgtaaaata attatcgatg atatgggcat
     5821 gtggtgtacc ttgtgtacat catataccta tcagacaaat aaaaaatata ttgtattttt
     5881 aggaggacat tttaaaatgg caaaagaaaa atttgaccgt tctaaaccac acgttaacat
     5941 tggtactatc ggacacgttg accatggtaa aactacatta acagctgcaa tcacaactgt
     6001 actatctaag aaaaacggcg gccaagctat ggcttacgat caaatcgatg gtgctccaga
     6061 agaacgcgaa cgcggaatca caatctcaac agctcacgtt gagtatgaaa cagatacacg
     6121 ccactacgct cacgttgact gcccaggaca cgcggattac gttaaaaaca tgatcactgg
     6181 tgctgctcaa atggacggag ctatcttggt agtttctgct gctgacggcc caatgcctca
     6241 aactcgtgaa cacatcctat tgtctcgtca agttggtgtt ccttacatcg ttgtattctt
     6301 gaacaaagta gacatggttg atgacgaaga attactagaa ttagttgaaa tggaagttcg
     6361 tgacctatta acagaatacg aattccctgg tgacgatgtt cctgtagttg ctggatcagc
     6421 tttgaaagct ctagaaggcg acgcttcata cgaagaaaaa attcttgaat taatggctgc
     6481 agttgacgaa tacatcccaa ctccagaacg tgacaacgac aaaccattca tgatgccagt
     6541 tgaagacgtg ttctcaatta ctggacgtgg tactgttgct acaggtcgtg ttgaacgtgg
     6601 acaagttcgc gttggtgacg aagttgaagt tgttggtatt gctgaagaaa cttcaaaaac
     6661 aacagttact ggtgttgaaa tgttccgtaa attgttagac tacgctgaag ctggagacaa
     6721 cattggtgct ttactacgtg gtgttgcacg tgaagacatc caacgtggac aagttttagc
     6781 taaaccaggt acaatcacac ctcatacaaa attctctgca gaagtatacg tgttgacaaa
     6841 agaagaaggt ggacgtcata ctccattctt cactaactac cgtccacaat tctacttccg
     6901 tacaactgac gtaacaggtg ttgttgaatt accagaagga actgaaatgg ttatgcctgg
     6961 cgacaacgta acaatggaag ttgaattaat ccacccaatc gctatcgaaa acggtactaa
     7021 attctcaatc cgtgaaggcg gacgtacagt tggtgccggt gtcgttacag aaatcaaagc
     7081 ttaattcact taagttttaa agtaaagcaa catttattcg gacgtaatcg ctcggaacga
     7141 aggtcttagg atctttgttc cgagcttttt tatttattgc aggatcagga aaaaaga
//
LOCUS       DAYYGS010000107         7129 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000107 DAYYGS010000000
VERSION     DAYYGS010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.110"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..676
                     /locus_tag="ACHA44_002482"
     CDS             <1..676
                     /locus_tag="ACHA44_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8905727.1"
                     /translation="GKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKN
                     TPSDKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEIL
                     IESLIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKII
                     YEICNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQIS
                     EGIDKQ"
     gene            752..970
                     /locus_tag="ACHA44_002483"
     CDS             752..970
                     /locus_tag="ACHA44_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905728.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            957..3650
                     /locus_tag="ACHA44_002484"
     CDS             957..3650
                     /locus_tag="ACHA44_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8905729.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMH
                     GQAKSAEELINQKRHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAK
                     NSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQG
                     TKTIDVRENLQSSSTSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDV
                     RENVQSSTSSQGFKTVDVRENVQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIID
                     SEIRENDKGSKPRRRFTYEDNELFRDTLNDPNPLFDSLLKK"
     gene            3664..3861
                     /locus_tag="ACHA44_002485"
     CDS             3664..3861
                     /locus_tag="ACHA44_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905730.1"
                     /translation="MQLILQISTHDFLELDELCESVKKATNEKITVTNYIELMIQEEL
                     KMKNEIESDFKCCSLEKNVRG"
     gene            3865..4476
                     /locus_tag="ACHA44_002486"
     CDS             3865..4476
                     /locus_tag="ACHA44_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HFM8905731.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            4461..5852
                     /locus_tag="ACHA44_002487"
     CDS             4461..5852
                     /locus_tag="ACHA44_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8905732.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            6068..6382
                     /locus_tag="ACHA44_002488"
     CDS             6068..6382
                     /locus_tag="ACHA44_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905733.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
     gene            6375..6869
                     /locus_tag="ACHA44_002489"
     CDS             6375..6869
                     /locus_tag="ACHA44_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905734.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            6946..>7129
                     /locus_tag="ACHA44_002490"
     CDS             6946..>7129
                     /locus_tag="ACHA44_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905735.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDRIYDFSSSLDLF
                     ELEAEIIENSREKNGKI"
BASE COUNT         2714 a          962 c         1391 g         2062 t
ORIGIN      
        1 tgggaagaat aatccaaatt tttattattt attgaaacca gaattcagcg aaggagattt
       61 ctacaaaaaa aagtgcattg aggaaaattt acaccttccg acaaatcaac attacagtcc
      121 aagtgaaaaa aatacacctt ccgacaaatc aacattcgat ggtgataagt cattcccgct
      181 tccaggtgaa aaatttttac aagaacttaa tcattcttct aaagatttta aagattttaa
      241 agaatctcaa aatgctgact ttattgattc actttcagtt gaaaataaaa aattagatga
      301 aatattaatt gaatcattaa ttgaaaaaga atggctttat caggtatggg gggataatat
      361 tgtaaactgt ctgaaattag tgggtaataa cgattacagt caattttatg actggtataa
      421 aaaattcaaa tatgcgatta aatctgctga aaaagaacaa aaaattcaac tgaaaattat
      481 ctatgaaatc tgtaataaca agtattttgt agattatgtt agagaacagc tatcaatgac
      541 tttacgtgac ttgatacgaa gagcaaaaac agatcaccgt atcaaacaaa aagacgatta
      601 tatctttgct tctctaaaaa ataaagcgtt agaactaatt gaattgcaaa tcagtgaagg
      661 aatagataag caataagaat agaattgagt caaatgaagt ggaggggtat ctccgcttca
      721 tttgagaact ttgaatatag gaggggttat tttgattgaa aatcgtttaa tagatcaagt
      781 ttggtgtcta gatcaaaata cagataatct cgaattaatc gagactattt gcttcaacac
      841 tatagttctc gatttaagat ctgaaaatga caattgtgta actcatatca taattgacag
      901 aaaaacagct cagaagctct ctgagagtct cagaaaatgg gctgagggag gtaataatga
      961 aaatagttaa agaaaagatt gccagtagaa aaatacactg gttagctttg agcatacttg
     1021 ttttaatgtt tatcgttgtt ttcaccggta atgttgtcca agcaagcatt ttcaattggt
     1081 ttgatgacac ggaaagtcaa actgaatttt tgaataatcc tgtctatcaa tcgtatttaa
     1141 aaaaatataa cggaaatttt tacagtcagt ttggattgtg gatagggtgg tctgttataa
     1201 aatcgatgtt tgcaattgta gatggtattc aaaatatgat tcccgaagtg ttagatttgt
     1261 ttagctttat tgaaagtacg ggaatgaata aagtttatca atcggtctta agtacagttg
     1321 ttattggttt aatgatactt tcactgatgt ttgttggtta caaaatgatt actggaaaag
     1381 gaacagtgga tttaaaatcg gttggaatga acgtagtgat gtctgtagca ttaattcttt
     1441 taatgccgac tttgattgga tcaacagaaa aactttcagg aataatgttt gcaaagactc
     1501 tttataatga cgcaactagt atcaataatc aagatggcgg tgtagcatgg tcattgattg
     1561 agcagggagt gacagattta gcttatatta ataagacaga tcaatattcg acaattgata
     1621 aaacaaatga ccgaaataat ctaacaaaag aaaatttctg gaaaacggat cttacgcaag
     1681 tattgacaga taatgtaatt gataaaatgg aaaaagagaa cccaacagct gataatctgc
     1741 gttatgaatt agttgaaaat tctgataatg aatttgttgc tactaaattt tcagataatt
     1801 ttttatcaac ctttgttgat ggtatcaaat caggatatta tcgctatcaa gcgaacctgt
     1861 ggggaatttc ttttggattg attgctttag cggtagcgta tgtttttagt gctttcatta
     1921 taattacagc aatactcgag ttagcattta aacgtgtact cggagtttta gtatttgcta
     1981 ctgacttaga aacaggacaa cgatcaaaac aagttttgtc agatatttta cagtgctatt
     2041 tgacagtcgg atttcaaggg ttcggtttat ctatgtttgc aatgttcata aattacctaa
     2101 atactggcga aggtatctca gcaaatattt ttataaaaac aattgcttat atctgtgctg
     2161 tgtttgtttt aatcaaaggc agtggaacag tcatgagata ctttggtgtg gatattggac
     2221 tcaaagaagg ctatgggcaa cttgcttctg cttttggaat gggtgcaatg ctattccgta
     2281 aaggaacagg tggtatgaac cggtctaaag gtgggaaaaa taatggtgga aacgatcaag
     2341 ctgaagggga agaacgaaaa cctgaacgca actttggaca atcactttct aaaaaagcta
     2401 ataaaacggg gaaagcgctt gggtatgctc atgaacgtgg attatcaggt ttagcttcag
     2461 atggatctac aatggctagt gaaagagcaa caaaaccatt taagagtatg cgtgatatag
     2521 ctaatgatac caagagtaag tttaaagaag gtttagatga tggtactgtg tccgctatta
     2581 ataaaaatag caaacctatg ttagctaaaa ataaagaaga tgaaactgga aaatatgctg
     2641 attccatgcc aacacgtctt tcggatagaa aagatggtgc taaggttgaa aatgccgata
     2701 gtattatgtc tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct gaatcgaata
     2761 ataatcctgt aagtgcaatt cagcaaaaag tccaacaaga tattgaagaa cgtaaaaatg
     2821 ctatgcacgg tcaagctaag tctgctgaag aattaattaa tcagaagcgt catgaagcga
     2881 aatacacgcc tgaagcaatg aatcgcgagg aaataatgtt aaggaaacgt gtagaagggc
     2941 ggacaggcgc taatattgac ggaaaagaag cgttgaccaa agaaagaatt caagaagcta
     3001 aaaattcaaa tacaggatta gaaaaattag tcaaagaaaa tccacaaaat tctacaagta
     3061 gtcatggtgg ccgatcagta gatgtccgag aaaatattca aggttcactg aatggtcatg
     3121 ttggacggaa tgttgatgta agagagaatt tacaaagttc ttcaaatgct caaggtacta
     3181 agacaattga tgttcgagaa aatctgcaaa gtagcagtac aagtcaacca aaaacagtag
     3241 atgttcgaga aaatgtacaa aattctacaa gtagtcaagg gtttaaatca gtggatgtcc
     3301 gagagaatgt gcaaggtagc agtgcaagtc aaccaaaaac agtagatgtt cgagaaaatg
     3361 tacaaagttc tacaagtagt caaggattca aaacagtaga tgttcgggag aacgttcaaa
     3421 aagatagtgg agaaatgaaa gaaaaaacac aaaaaatcaa tatcgttgaa gacagaaagt
     3481 cagcgaaaaa atttgattca aatcatgaaa cagtgattat tgatagtgaa atccgtgaaa
     3541 atgataaggg atcaaaacca agacgaaggt ttacctatga agacaacgag ttattcagag
     3601 atacacttaa tgatcctaat ccacttttcg atagtttact taaaaaataa agaggtgatt
     3661 gtcatgcaat taattctaca gattagtaca catgatttct tagaacttga cgaattatgt
     3721 gaatcagtaa agaaagcaac aaatgaaaaa atcacggtca ctaattatat tgaactgatg
     3781 attcaagaag aattaaagat gaaaaatgaa atagagagcg atttcaaatg ttgctctcta
     3841 gaaaaaaatg tgaggggata atcaatggat gataaacaaa aaatgtacga aaaaaatcgc
     3901 aaacatagtg aggccttaaa ttttcgtgtc gataaaaaga cttatcttga actgacattg
     3961 attgcaaaac aaaggggaat ttctagagca gacgtttgcc gtgagcggtt aaagggactc
     4021 aaatatgaac cgccgttgtt agctgaagat gatagggtag ttttaaccaa acagttaaat
     4081 caaattggta acaatttgaa tcaagcggta aaggcattaa atatcattgc taaatatttt
     4141 agagaagaat ctgaagataa aaaagaaaag agcctacatt ttagttttga tgctagccgt
     4201 tacaacattt ttaaagatga acttgcgaat agcatagcaa aaaagttgat gattagttat
     4261 ccagatgaca tttttttgga gtccaatcgg agtgccccct ctttttttaa attatcagaa
     4321 gtagaaaaag aatatttcga atattggata gaaaagcaaa ctagaccggg tacgaaagaa
     4381 gagtattccc aaaaagttga tcggaattta attcactcaa ttcagattat tgcaagttta
     4441 aaagatgagg tgaaacaact ttgggagcta ctataacatc gaaaggacgc aagaaaaatt
     4501 tatcctcgct attacaatat gcagctaatc cttcaaaaaa agaaaaatgt gttgcagtct
     4561 caggaataaa ttgtagtgat gatagtgagg aagcactgca agaaatgaat ttagtacgta
     4621 ccaagatgaa taaatctaaa gatatagcac aagcgtttca tgttattcat tcgtttgata
     4681 agagtacttc taaagagtta agtattgata aaatgcacga aatagctgtc gaatttgcag
     4741 agaaagcttt tccaaattcg caaatcattg ttgcaagtca caatgataaa gatcattttc
     4801 atagtcatct tgtcattaat aatattaaca tggaaacatg taaaaggttg cgtattgacc
     4861 ctaaagattt agaactaatg cgaaaaatta acgatcaagt cctcaatgaa catggtcttg
     4921 aatcagtgaa taatgagtac tatgaaaact tagaaaagag aaccaatctt tattcaaacg
     4981 atccttctaa gatgaaatat ggaaaaatta attatcaaga gtttgtgcaa aatgcgatcg
     5041 aagcaacgtt ggaaaaatgc gaagtgaatt cttttgaaga tttttcggat catttggcaa
     5101 aagaatataa cattgaagtt tatcaatttt caaaaacaaa taacaaactt ggctatgttc
     5161 tttacaaaca aggtgtaaat ttctatgagc gtagaggtga gttaattact ttaggaagag
     5221 ataaacaatc tagaaaagaa aaaagtcagt atgtcgaacg tacttttagt gcaagaaagt
     5281 tagggaaaag atattcattt gaatctgttt ctgaacaact tgaaaagaat ttagaagaat
     5341 cacatataaa aaattttttt aatatggaaa gagagcaagt tatgtcgcta attagtggag
     5401 taaattacaa aactggtgag ttaaatgtac cttttgtgga attagattcc atcgcacaaa
     5461 agcaatttag cgaccgtgaa ggcgatgaaa aattcattaa atatgatgac ttcttgcgag
     5521 aggtagacaa gcattttgta aacacaatgt ctttagaaga tgatcaagaa tacacgatta
     5581 aacttcatac ggatgtgaat cctcaaactt tacgtagtgc gaaaagtaaa acaatttcta
     5641 ttaaaaagac tgatcagaaa gagttatttc taccggcaaa gggttttgaa aattgttctc
     5701 atacaccttc tgctaaacag attcactatc aaatcagtcg cataatgaat gacgatgtaa
     5761 aaatgtatcg tgctaaaaag gattatgaat tcacacaaca aatgattgaa aatcagcaaa
     5821 aaaggcaaca aaattttgaa atggaattct gaatgggctt gttagtggac tttccagttt
     5881 tttgcctatg aagagttgct tggaaagaag cagtttttag gacatacgcc agctacccat
     5941 ctacttgcca gtcaatccgc cccgctcacg ctaggctact ttctggcaag tcagatggtg
     6001 ggtaaatctc tgagggagca agctccctac agactccctc ccaaaaaaag aaaggtggaa
     6061 gaaaaaaatg aactatccat attttaaagt ctcggcttca gaagaaacta aagaaatatt
     6121 caacaatttt tacaaccaaa acaaaggagt ttttggttcc aaggcaaata tgtttcgtgt
     6181 tatggtttca aatttaccag tgttagctag tccatcaaat aataaattta atgattctga
     6241 aagcattaaa tttgaacaaa aaatttcaga actagaatca atgataagta atgaagtaat
     6301 tgaaaagctt gatgacattg atcaaaagtt atcgtattct ctaaaaaaca aatacaaaac
     6361 ggaggagaaa aaagatgttt aaagtttact tcccgtttca gtggctcgtt aaaggaattc
     6421 ttttagtgct actaggattg attctttttg gaagttatct aacaattaaa gccagacatt
     6481 ttccagaaga aaagccaata aaaattgtaa caatagattt aaaaataggt gaaacttatc
     6541 aggcagatgg ggttgaacta acatttaaaa atattgaagt gaccgaaaaa agaaatgaat
     6601 ttgcacaaca tgatcgtgta ttacaagtga cctatacact taaaaatgat acagaaaaac
     6661 caatacgtcc acaacaatgg tcgattgaaa ataaggatgg tgaagagctt tatgagtatg
     6721 ctatgatatt agataatcca gagtatattg aaagtgacaa agaagaaaca gtaaattact
     6781 attatgaatt accgattgaa aggcaggact accaaatcgt agtacgtaga gcgaatggtt
     6841 attttgttgg attacccttt actttgtaag tcttattttg ttagtggctt ttaagttaat
     6901 atcatttttg tgatttttga aaatgaaagt gaggaaattg gaagaatggc tgaagaaaat
     6961 aaagttgttc cgtttgaaac attagtgata aataaggaag agtttgagca actttttctt
     7021 gattttttta ttcaatatga caggatttat gattttagtt cgtcactaga tttatttgaa
     7081 ttagaagcag aaataataga aaacagtcga gagaagaatg gtaaaatca
//
LOCUS       DAYYGS010000108         6987 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000108 DAYYGS010000000
VERSION     DAYYGS010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.111"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(1..1634)
                     /locus_tag="ACHA44_002491"
                     /pseudo
     CDS             complement(1..1634)
                     /locus_tag="ACHA44_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2073..2942)
                     /gene="scrK"
                     /locus_tag="ACHA44_002492"
     CDS             complement(2073..2942)
                     /gene="scrK"
                     /locus_tag="ACHA44_002492"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8905736.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(2962..4083)
                     /locus_tag="ACHA44_002493"
     CDS             complement(2962..4083)
                     /locus_tag="ACHA44_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HFM8905737.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(4085..4480)
                     /locus_tag="ACHA44_002494"
     CDS             complement(4085..4480)
                     /locus_tag="ACHA44_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8905738.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(4499..5296)
                     /locus_tag="ACHA44_002495"
     CDS             complement(4499..5296)
                     /locus_tag="ACHA44_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8905739.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(5298..6086)
                     /locus_tag="ACHA44_002496"
     CDS             complement(5298..6086)
                     /locus_tag="ACHA44_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8905740.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(6100..6576)
                     /locus_tag="ACHA44_002497"
     CDS             complement(6100..6576)
                     /locus_tag="ACHA44_002497"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905741.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(6648..>6987)
                     /locus_tag="ACHA44_002498"
     CDS             complement(6648..>6987)
                     /locus_tag="ACHA44_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8905742.1"
                     /translation="FSLTNLMEQLTILNPTKLLERVSDAIYILQNDLGISFDNNTCFG
                     LYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFKQSFSVVEHYYSVDIPIHEV
                     KYVYDYVKRA"
BASE COUNT         2252 a         1443 c         1048 g         2244 t
ORIGIN      
        1 agatcattat ttttatatgt tgtaacttcc tgataagaaa actaattaaa agaaagaggt
       61 aactacataa actaattaaa aaagctaact tcatagttgg aggataaatg gaagaaactt
      121 caatgattgt ttctccttgt gttccttcaa tcgacactag tcctattatc aatctctctt
      181 ttttgtccat ttacttttgc gactgttccc caataatcaa atacaggaag cgttattttt
      241 tgtcctttag attcatgtac tgtatagact attttcgaat ccatcacttg ataaggcata
      301 gtatgccatt ccttgccaat tcttacttca tgattctgca aatgttcttt gtattgtaat
      361 gcttccgtgg gtgcatagtc ataaagtcct ccgggaagga gtgtcgtgta acgaataaga
      421 ttttcttgat tcattaccaa gtgatctctc tgagtgacaa ctttatgttc atagtttgct
      481 tttgttgcag cagatacagc caacagtaat aaaacaattg ccgttatatt aactactttt
      541 ctttgcttag ctgatgtgct cttcatgaaa acgaacgata aaatcaacga gccaaacagt
      601 acttgaaagc ctaagattcg ccaaggaaat tgaatcattt gaatgggtgt attttgtaat
      661 aaagaccatg gaaaaaattt ggtggataag agccaagaga ctaccgcact aaggtaaatc
      721 actttttctt tgcgattaaa tcctttaaaa aagaagagtg gacagatcaa agaaataata
      781 ccaatgattc ctaccgtaaa ggttgtcagg tcattattca tcgcaccaat caaactatcc
      841 gagacattta atgcttggtg ttgtaaggtc gttttatctg gctcattaat cgattgaacc
      901 atgatttgtc ggaacatcgg ataccaaaag tatgctgtta aaagaatcgt cattcctact
      961 gcctttgaca actccacaat caaccggatg ctttcttttt ttcttgtttc acttactaca
     1021 aagccgccta agactagtaa gccaacggct attacactca taaaagtgga aagaacatgt
     1081 gtataaatga gtaagctcat actgactgct aatggaattg cacatggctt tttctcataa
     1141 atcagtgcgt aagcataata cataactatc ggtataaccg taagtgcaat cgcttcagca
     1201 atagcagaac gatgataaat atcaatcgta cggtacccat aaaatgtata taaacagcta
     1261 aatataaaag ccgcatcgat tctttttaaa aattttagtc cataatgaaa catcagcatg
     1321 atagtacaaa cattcaatag ccaaacatat aagatataac taacgattaa attattactt
     1381 atccaataaa aaatgactgc cggaaaaaag gtcaagtagg gataaaaata attcactccg
     1441 ctcccataat gattaaacgt cgtaaaattg attggaccag aaaaaattga agataaccct
     1501 ttgactctag caaaatggaa ggcaatatcc cccgatgtag aaatagtatg aacatttcta
     1561 aaataattga ttgaataaag tccagcaata attgctagga gaatggacaa tattattttt
     1621 ttctttactt gcattactta attatatact cctttcatgg attgctttat ctagtataac
     1681 ttaaatttta aataaatcgt tatttatttt tactctcact taaaaaagca ctaaaattat
     1741 aagagaggga ggtaatggtc tgaacaccaa gaattgttcc aacatcgaat tgcttaacta
     1801 atgagttcaa agcttaatta tgtggctctt atatttaccg agcgtggatt gcgatctctt
     1861 tagcgaaaca aacatctttc ataaaaaaaa cagccatatc cgctttcttg gatatgacta
     1921 tttactacta ctttactatc tttcattctt cttgtctcac ttatgataag gcgctgggta
     1981 tcatgagtga atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt
     2041 cataagcatt ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct
     2101 aaagttaagc atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct
     2161 tcgatatttg gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc
     2221 tttttcagat gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc
     2281 atcagggtcg tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga
     2341 tcttctatag aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat
     2401 gccaaccctt cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa
     2461 tgtctcttaa ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca
     2521 tcttgtatag ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa
     2581 ccttttccat gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag
     2641 gcaattggaa tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca
     2701 gtttttggag tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt
     2761 ccaaatgacc ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt
     2821 accaaactca ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt
     2881 aaatcatcat tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac
     2941 atttcttact ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc
     3001 gaaaaactca tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg
     3061 ctcataaagc ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt
     3121 gaaactaatg gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc
     3181 gccaaaacca tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg
     3241 atctctttta gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc
     3301 cctttctcca aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc
     3361 tgtagatgtt attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc
     3421 cccccaccag tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt
     3481 gcctttgatt ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt
     3541 ccatttagca tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt
     3601 ataaaaaatg tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt
     3661 tcgaaatggc atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa
     3721 ggaattcact tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc
     3781 tacagaagga gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa
     3841 actcccagcg attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc
     3901 ttccatttct gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt
     3961 tctcccaatt cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt
     4021 ttctggtgaa atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt
     4081 cattttattc gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt
     4141 gttgtgcctc cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga
     4201 ttgacattgc caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg
     4261 taaaaaactt ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg
     4321 tagtatccac taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac
     4381 ataagctact aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct
     4441 cagctagtat tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt
     4501 acgctaatat tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa
     4561 tggtcatact gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg
     4621 gaattaatgt tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa
     4681 aagtgaattt aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg
     4741 ttagccccaa tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa
     4801 caatcttcat tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta
     4861 ccatgattaa aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag
     4921 ccgcaattga tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag
     4981 caattggacc catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt
     5041 gcttttcctc cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat
     5101 tgtaatactg aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt
     5161 ttcttaaaat aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa
     5221 aggtacaaac tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt
     5281 gatatttttc gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca
     5341 gaagcagtga tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat
     5401 cctatgatcg ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca
     5461 agtaaataac taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca
     5521 gcaggcaaca tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc
     5581 aaaacgtaat taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca
     5641 gataaaccaa agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt
     5701 ttattcgcat attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca
     5761 tctagttgca cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca
     5821 agctggctac caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag
     5881 ccaccaaaac cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct
     5941 gtttttatat cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca
     6001 aagatcatcg gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata
     6061 ataatttgaa aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat
     6121 ttcctgtgcc ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa
     6181 ttttttatac gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc
     6241 attatcttta acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac
     6301 taaatgaagg attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt
     6361 ccccttttta aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc
     6421 aggtctagct aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat
     6481 caccatcact ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa
     6541 taaacggtta tcaactctcg acataacaat actcataaat acttcctcct ttatctttca
     6601 ttttcttaca agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt
     6661 acataatcat aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca
     6721 acgacactga aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca
     6781 ttgaaataaa tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta
     6841 atgtgaacat ataatccgaa acacgtatta ttatcaaatg aaattcctag atcattttgt
     6901 aagatgtata tagcatctga aacccgttct aataatttag tcggatttaa tatggtcaat
     6961 tgctccatta aattcgttag actaaaa
//
LOCUS       DAYYGS010000109         6888 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000109 DAYYGS010000000
VERSION     DAYYGS010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.112"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..182)
                     /locus_tag="ACHA44_002499"
     CDS             complement(<1..182)
                     /locus_tag="ACHA44_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFM8905743.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="ACHA44_002500"
     CDS             517..951
                     /locus_tag="ACHA44_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFM8905744.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="ACHA44_002501"
     CDS             974..1294
                     /locus_tag="ACHA44_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905745.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="ACHA44_002502"
     CDS             1435..1626
                     /locus_tag="ACHA44_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905746.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="ACHA44_002503"
     CDS             complement(1697..1954)
                     /locus_tag="ACHA44_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HFM8905747.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="ACHA44_002504"
     CDS             2112..2375
                     /locus_tag="ACHA44_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905748.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="ACHA44_002505"
     CDS             2399..2587
                     /locus_tag="ACHA44_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905749.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="ACHA44_002506"
     CDS             complement(2670..2855)
                     /locus_tag="ACHA44_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905750.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="ACHA44_002507"
     CDS             3070..3441
                     /locus_tag="ACHA44_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HFM8905751.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(3591..3663)
                     /locus_tag="ACHA44_002508"
     tRNA            complement(3591..3663)
                     /locus_tag="ACHA44_002508"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3628..3630),aa:Gln,seq:ctg)
     gene            complement(3709..4137)
                     /locus_tag="ACHA44_002509"
     CDS             complement(3709..4137)
                     /locus_tag="ACHA44_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905752.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(4197..4955)
                     /locus_tag="ACHA44_002510"
     CDS             complement(4197..4955)
                     /locus_tag="ACHA44_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFM8905753.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            5250..5447
                     /locus_tag="ACHA44_002511"
     CDS             5250..5447
                     /locus_tag="ACHA44_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905754.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            5553..6035
                     /locus_tag="ACHA44_002512"
     CDS             5553..6035
                     /locus_tag="ACHA44_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905755.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(6093..6689)
                     /locus_tag="ACHA44_002513"
     CDS             complement(6093..6689)
                     /locus_tag="ACHA44_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8905756.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
BASE COUNT         2331 a         1059 c         1354 g         2144 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga atacgcccga ttttattatt
     3481 cataatttaa ttatgtaaac taaatacaag tagtaaaaat aatacaataa tccccctgca
     3541 aaaagattgt tcttttacag ggggttcatt ttttcagcaa gcacatcaaa ttgggatggc
     3601 agggttcgaa cctacaattg cttgattcag agtcaagtgc cttaccagtt tggctacatc
     3661 ccatttattc aacaaaataa tcatagcact ttcctaaaaa aacgcaagtt atttttcttg
     3721 atcggatagt tgttgcttta ctcgtaaaag gcggcgtact agtttctttt ctaatccagc
     3781 aggaagggca atcagttctg ctactgacaa gacggtgtcc aataactgat attctttgac
     3841 aggttcattc ataatatgat agatgtttgc taaacgttca atatattcaa agcgagtata
     3901 ctctgttcga gcaattagaa atttcaataa atcggtggct ttctcgtact cttgatctgc
     3961 gtaataaaca agagattgac gatacaattt gatttccagt tcattgaggg tagcaggtct
     4021 gcgacgctcg ttccactcat taatagagac tttaccttga acatttccct tgcagtcacg
     4081 gacaaaaata atcggaattt catttggatc cattttttta tgtgtgaatt tttccattat
     4141 ctacgctccc ttcaaaggtt tggtatcttg agtataaggg atttttgttt gaattactag
     4201 cttttctcca aagttagcat ctgtccaccc attgcagtga aaatcgccga aaaatcgttg
     4261 atcggtactg ctcgattttt tacaccatag ggatcgttga cataaatatt ttctttgtca
     4321 tatccagtga ctacacaagc atgaacaaga ggagtgactc ttacttctcc attatttgtg
     4381 gtccaagtca taaaatcagt ttcatctggg acttcaaaat tgatagtcac agaagtccag
     4441 acaggatttc ctttagatag tgcttttttg atatcatcga gttgagcatc ggtttttgga
     4501 aaagtgtgat aatccgtttg gacgatttgc tgagcaaggg aggcaactgg gggaacatag
     4561 acgaccatag cccagtctcc gccactgata tcaccaacaa atccttcatt aggatcgcca
     4621 tgagtcccat ctgcattgaa aacagggacg taatccaatt gctcagctaa ctggttttta
     4681 gttgtttcat aaccataaaa agataaaagc atagaaagcg aagtgacttc acagccattt
     4741 tctaaagaag gagaatcaaa ctggctctct aatggaacat ctagtaaaac ttctgctttc
     4801 tcttttgtct ttttcgtttt ttgtgttgaa cttttttgtg cagtgttcat tgtatgttct
     4861 ggagaatgga tatgtaggta ataacttgta ccgcttaata aaagagcagc tgtgccccct
     4921 agaaacaata gttttttgaa cgtttttttc ttcatttgaa atctcccttt tcagctgttt
     4981 atctttccta ttataaatgt atttttattg gaaatggaag tattatattt taatatgtag
     5041 gaaataatta gtttttaagg aatatgtttt aattttttga aaaaaattct tctaaataca
     5101 acagctgttt taattgtgaa aacgaaaaca gtacgctata atgcctgtaa ggttgatttt
     5161 taactgtaaa taaaaatcaa tgagttaaat ctccaaagaa atagcagtgg taatgtggag
     5221 aaaagttcca gcagtttagg aggattttga tgacggaaga aatgattaat ttaggagaac
     5281 aatatgcatg caaaccaatt ggttttacaa aaacggtcat tggtgaagtt gtatcaaaaa
     5341 tgacaaactg cgccgtggtc aaagttgccc aatgtgctgc agaggaccaa gaattattag
     5401 atgaaaaagc aagcatggtt gttgcaaaat atgatacatt tgaataaaga aaacaattgg
     5461 acgtgcggcg tgctgcaagt ccaatttctt tgagaaggta aatcagtatt cttttcctat
     5521 tttctggaga agaaaaagcg aggcgagttg tcatggtaac attctttacg gttctattgt
     5581 ttttattcgt tgttgtgatg tttttatttc tttggctggt tcgaaaagaa attatttatc
     5641 ggactgtgcg aaatcgatgg gtctacttgg tgataccctt cttagtgtca ctagtcattt
     5701 ggtatacact catcagtcat cctacagcag atgaattggc aaaagggatc ttgtcagcaa
     5761 tgatttttat tagttttttg ttggacagta gaggaatcac agaagaagga cttgttttga
     5821 atagctttga caaaaaaggt gttccttttt ctgagatcaa taaaattgta ttatatcagc
     5881 caaaaggttc taaaatcgta aaaatgaatt ttttccgaaa cggatggcga ggaccgatgc
     5941 tgaaattttc tgcatcatta gaagaattag ttccattttt gtcccaacgc ttaaacgatg
     6001 aggcagagat tgatattatg attgatccag aataaaaaca gaggttgcga caagatgttt
     6061 gtcacaacct ctgtttttta tttcaaataa aattatccaa atacgcggaa gcgatcggca
     6121 tcgttcatgt attctttttc ctcagccggt gtttctggtg aaccgaatac taattgcgaa
     6181 cgtagtttcc agtttgaagg gatattccat tcttttgcaa ctgcttcgtc aataactggg
     6241 ttgtaatgtt gcaagtttgc gcccaatcct ttatcaacta atgctaccca agtattagct
     6301 gttgcgattc cgtttgattg ttctgaccaa tctgggaagt tgtctgcata taattcaaat
     6361 tgttcttgta gaccttttac cacgtcagta tctttgaaga ataatactgt accataacca
     6421 tttttgaaac cagctaattt gttttgtgta tttgggaaag cttctgctgg agtcaatggg
     6481 cgaagtgctt cttcagtgat ttcccaaagt ttttcatgtg catctccgaa aaggataact
     6541 gcgcgtgttg attgagcatt gaaagcagtt gggctttctt taattgcttc tttgatcaaa
     6601 gttgttaatt cttcattaga aagtgaaaca ttacgtccta gatgatagat tgaacggcgg
     6661 tttttcaaag tgtcagtaaa agttgtcata ataaaatttc tcctttgatt tgcgttttgc
     6721 atttttttaa ttgattacga aatgaagtat agcaccttac aaaaagtaag taaacagata
     6781 tgctcacaaa aaataagcga agttcaaaaa acaattttaa taagaaaaat cgttatgtaa
     6841 ggattttctt agttatcgct ttcattgttg tgttttagga atttttgc
//
LOCUS       DAYYGS010000110         6835 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000110 DAYYGS010000000
VERSION     DAYYGS010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.113"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(169..1935)
                     /locus_tag="ACHA44_002514"
     CDS             complement(169..1935)
                     /locus_tag="ACHA44_002514"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905757.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1895..3649)
                     /locus_tag="ACHA44_002515"
     CDS             complement(1895..3649)
                     /locus_tag="ACHA44_002515"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905758.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(3759..4328)
                     /locus_tag="ACHA44_002516"
     CDS             complement(3759..4328)
                     /locus_tag="ACHA44_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HFM8905759.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(4346..5761)
                     /locus_tag="ACHA44_002517"
     CDS             complement(4346..5761)
                     /locus_tag="ACHA44_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905760.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(5772..6440)
                     /locus_tag="ACHA44_002518"
     CDS             complement(5772..6440)
                     /locus_tag="ACHA44_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HFM8905761.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(6570..>6835)
                     /locus_tag="ACHA44_002519"
     CDS             complement(6570..>6835)
                     /locus_tag="ACHA44_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8905762.1"
                     /translation="GTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIH
                     FYIYSFYATFYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
BASE COUNT         2328 a         1243 c          916 g         2348 t
ORIGIN      
        1 aactttgatt attccttata ttataagaat agacattaag tgaactaatt caatcatact
       61 gattctttcg tgtattcaaa attagatgct gtttaataaa attattcttt ttgaaaaaga
      121 attataacta tatcccaaaa attgtaggtt aatttaaagc ctatttcatc atcgtgattg
      181 agcttctttt tcatagacga atttctcaga tagtttagtt ttctttatga gatttttgta
      241 tatttctgac cttcgataca actcattatg agtaccttga tcaactagtt ttccttgatc
      301 catcactaca attttattta ccttttcaat agacttcaag cgatgcgata taattagtac
      361 tgtcttatct tgtattaaac gatttaaact ttcctgaata cgtttttcat tttccgcatc
      421 taaactggca gtaatttcat ctaaaattaa aattggcgca tttttcaaaa aagcccgtgc
      481 tatggatagg cgttgtcgct ctcctccaga aagtgaactt ccgttttctc cgaccaatgt
      541 atgataaccg tcaggtagtt tttcaataaa gtcttcgcag ttagctaaac gagctgcttt
      601 tttcacttcc tcatctgacg cttgactatt tccaatacgt atattttcca aaatacttgt
      661 attaaacaaa gttacctctt gaaaaacaat agcgattttg ctaaacaaag aagctggaga
      721 tacacgttta atatcatacc catcaattaa aatacagcct tcatcataat catacagtct
      781 agacactaat ttcaagatac tagttttccc ggaaccacta gcacctacta acgcagtcac
      841 ttctccttgt ttagccgtaa aagagatatg atctaaaatt ggggtattgt tatcatatga
      901 gaaagagaca tctcgcaact caatgtcaaa agatttcaga ggactatctg atccctcttg
      961 gatactggtt tccttcatag ctctaattcg ctgtatttta ggagctaaat aaaatatttc
     1021 aagcactgct tctttcatag aatcgaagga atcctttatt tttattgcgg ctagtaagta
     1081 accaacgacg tagagtatag tcacttcacc cgttaacagt aagtgaacac ctactagaat
     1141 gactactgct aaactaacat atgagaaaat agaggataag gccatgactg aaaaagtact
     1201 taattctacc tttaaatgaa tcctttcact ctcttccatt tttttgaaaa gtctgtcttg
     1261 aactttttta gatagattaa agctattaat ctcttgctgc aattctatag tttcttgaaa
     1321 ttcttccgag ttctctctca aagtatcgta atatttagta ttggctttcg tttgggattt
     1381 ctttgataac aagattaaga caaaactaaa taacgttggc aaaataacag ctaatcccat
     1441 tttgacatta ccaaccaaaa gcatcactga aataaaaggg aaaaacagag ccataccgcc
     1501 ggctttaggt attgcatgac tcatcgcatg ctcaatacct tcaacgtcag acatgatagt
     1561 ttgagataaa tctgataaat tatgttttga aaaataggat aatggtaaat ttgacaaatc
     1621 gtctgcaatt tgcactctta aatgcgcact ttctttataa gtactattat ataagttttc
     1681 atactcccta tctaacaacc aatacatgaa aagcaaagta acaaacgata cggcaaaata
     1741 aagccaacgg ggctttgtat tttctaaaac taattcgtct atcagcatca ttataatgaa
     1801 tgctggtacc atattaatgc agtatacaaa aaaactagaa atagaagctt tagttaaagc
     1861 ttctgcgccc tgatcactta aagcaaattt gtttttataa aaacttttca ttgtcaagcc
     1921 tccaatcatt tgcattctca taattagtga ccaattgttt atacacttga tgtttagcca
     1981 ttaattcatt atgatttcca cgttctacta ttttcccttc tgacaagaca agaatttcat
     2041 ctacagattt aatgctggat aatcgatgag ctatcataat aactgttttt tctttcatca
     2101 gatgttgaaa agccttttgt agttcatact cattatctgg atcaatagat gcactggctt
     2161 catccataat aacaatcttt gcatctttta aaattgctct ggcaatagct atacgttgtt
     2221 tttctcctcc tgacagataa actcctttac taccaattaa ggtatgctct ctttcgggaa
     2281 atttttctaa aatcgattcg caaccggcta atcttagcgc gtcaaatact ttacttgctg
     2341 gcgcttgagg atttgctata gaaacgttat cgtaaatact ctgattgaat agtttcgtat
     2401 cttgaaagac aaaagcaatt gtgttaataa ttgtgtcttt tgaatagtga tttaaaggct
     2461 taccaccaat tttaatctgt cccttatcca cattgtagaa acctgaaatt agtttagcta
     2521 tcgtactctt tccgcttcct gattttccta ctaatgcata aattcttttt tgttctaaaa
     2581 ctaagttgaa attttctaaa acacactctt ctttgtatga aaagctgaca ttttcaaatg
     2641 caatgtcaca atttttaatc tgtgtctcgt tcccatattt tagttggtct tgcttcattt
     2701 ctaaataaag ggagtctagt gtttcgactg catatcttgc ttcaaacaag tgcatactta
     2761 catacattat tctcatgaaa gaaacaaaaa taattccact taaaaaaaac aacatcaaca
     2821 agtcaacagc taatatcttg ggagtggtaa gagaagttat aaaaaaggtt ataggaacta
     2881 tcaaaatgca aactatgcca aaaaatagcc attgaaaaag aacaaaaggt gttttcccac
     2941 tcaaagaata cttatatgca aacttagcat aatcgttaat tgctttattc aaggctttaa
     3001 atgatgttac tttagcacca aatatcttta taacttgaat tccgcggata tattctaccg
     3061 tttcagagct taacacatct aacgattctt gatatttttg aataaaattt gtattctctg
     3121 tcattttctt taacaagaat acaccaatta ctaacatggc gattagaacc aatccaattt
     3181 taaagttaat gaaaaagcct aaggcaactg ataaaacagg tgttactatg gcttgagcac
     3241 tatctggaat caagtgagca attgccatat gtgttttagc cgcgttgtca tcaattgtct
     3301 ttcgaatata tcccgaagaa tttaaatcaa aaaaacgaaa acttgcttct gttaaaccgc
     3361 taattccctt ttttctcaaa ttagtctcta agcgaaaccc aaggatatga gaaaataagc
     3421 cagaaaaaat ataaaataaa gctcctaaag ttaaccaagc aacagtttga accgcataac
     3481 tcttagctaa atattcatct cctacaataa ttagagactg aagaaatttg taaatactgt
     3541 aatatccata gactgttaat acgacagaaa aatcagaaaa taaagtggct aaccatccgt
     3601 agtatttcat tttagggaca taactaaata attttttgta tactttcatt ttttcctccc
     3661 ccgcattcaa tttcttctct tattcgttta caactgttaa tgctttccgt aatagcgtaa
     3721 attagagatg tacaaacaga taaaaggtaa actacgttct attttttgta tttttttaag
     3781 acttttcttc caatcaatac accaatcata gataaacttg ctacaactaa cactgaaatg
     3841 attccccata tacctaccgc atagtggatc attgcatcca ttgttgcagc agattgtcct
     3901 ttatctatcc aatcttgacg aaaacgatct gtaaagaacc atataggaat ccactgtccg
     3961 gcagcactgc ctaattgcat tagagcccaa gacgcaactt gaggaattgc tcgatcatat
     4021 ccaaatacag atagaataat atcagctaag atcgccatac taatcgaact gataaaccaa
     4081 ggtaagtacg ccccgcccat taacgtaaaa aataacaata aaacaattgt gtaaatagta
     4141 aaaattcctt ttctagggta gttatagcta atataaacaa atatgattcc agataataat
     4201 ccaaaaatcc cgggggatat gctgtgacca aacgccccac caatactact aaccattatt
     4261 gaaaaaaagt aaattactag ggaaataact gtagctaaac caataaaaat ataatctttt
     4321 gttttcatat atattctcct caattctatt tcgttatgtc attaaaaatc tgttctatca
     4381 aagttgaatc ttctactcta agttttatat ctttttttat tccaccattt tttaaataaa
     4441 taattctatt gcatgtttta taaagaaatt ccggatcatg agtagctata actactgcac
     4501 ccttttcttt taagtcttta ataaggtcag caatcctttc catattaata taatccaacc
     4561 cacttgtagg ttcatcaaat agaaataaat tagtttttga caatgaagct atagaaacca
     4621 ataatctttg tttttctcca ccggataaat caaacggtaa ttgatctttc attttataaa
     4681 gttccatttt ttttaaaatt ttttcttgct gatctttact aactatttca ttccccaggg
     4741 ctaactcgct cctcactgat tctgtgaaaa attggtaatc catttcttgc attactaaaa
     4801 acggcgatga caaatttttt ttattttcga ttatctttcc tttggtaggg cgaataatct
     4861 tcatgagtat tcttagtaag gttgttttcc ccacaccgtt tttccctacc aaaccaataa
     4921 catctccaac tttgacatca aaatttatgt cttttagaat attttcataa gtaatttctt
     4981 caacttttaa tacatctgca cttacctttt cgatataggg aacttcaatc gaactattaa
     5041 aaatgtcaaa tttccttaat gcgttattgt tttcagtttt taacaactga atagttgtag
     5101 tttttacagt tctattttta acaaatagaa cttgatcaat taattctttc aaatagtaaa
     5161 aacgatgttc agctataaca atagtaaatc ctttattttt taaatcacaa agtaagtcag
     5221 caagtttctt agtcgctttg aagtccaaat ttgaagacgg ttcatccaat ataattaatt
     5281 tagagtttag cgataaacta catccaatag aaattttctg ccgttcacca cttgatagcg
     5341 aaaaaatgtt tttatctaat atatttgaaa gatctaataa ttcagctaat tcattgattc
     5401 ttttatccat atgctctttt gtaaatccaa agttttccat cgaaaatact aactcactcg
     5461 ttgtatttgt tgtaaagaat tgtcctctag gatcttgact tacgtagcca atattttttg
     5521 atatactttc ttgtgttaag gattgtagcg agttattttc aaataaaatt tccccaacca
     5581 attttccgtc ataaagtctt ggaataagtc cattcattaa ctttaatagt gtggatttcc
     5641 cactcccaga agaaccagta atcaatgtaa gactgccttt ttttattgaa aaattaaggt
     5701 caacaagcgc ttgttcctta acatccttat atgaaaaaga aacattgtta aattcaaaca
     5761 attctttcac ctcaaatcaa aatacttaag aaaaaaatag taacggaaaa caggatgata
     5821 tagtctaata agctcatgtt tgttttatag taagaacttc ttttcacagg agattctatc
     5881 ccctttgtta ttgcattagc ggtcatttct tcagcaatca tagttgcacg tataagaacg
     5941 ggaactaaca gaaattccaa atattgcaga ggctgaaaaa aagacaagcg aatgtttctc
     6001 atttttaaag attctttaat cattcttatt tcagaacgat aagtcggaaa gaatctgatc
     6061 actactgtaa tacttaacat tatttttttc ggtaaatgta ttttttctaa agaggataat
     6121 aattcattgc tagtatagac actaaccaac aaccatgaga tcattacgac cggcaacatt
     6181 cgaaatacaa tatataagga tgtcgcaaaa aatatagtaa aaaactctat cttgatagca
     6241 tcaataattt tcattactaa aaacagaact gaataaatca agatgaaata aatacatgtc
     6301 tttattttac gaaacatcaa tactaaaact gtagcaataa tgaaacaccc cacttgaaat
     6361 atatttccct ttcctaaaaa aataatgacc ggaaaaaaaa tatttaaagt aatcaaggtt
     6421 aacggattga tttttgacac tctttcacct ccaaaaaata agatcgcact gttatcttaa
     6481 gctgataaaa atgcaccgtt attcagataa cagtgcgatc ttatcaaaaa gcataactga
     6541 gaacaattct caatgcaagc tttttttaat taactcagta attcaatcca cccaggaaca
     6601 gaaaattgat acaatcttct ggttaatagc atagtctttt cttttgaata gctatgcttc
     6661 aatatatcat agaatgtagc gtagaatgag taaatgtaga agtggatttc ttcttcatta
     6721 aagactaaat tttgattgat cattccgata gtctttaact tatctagaca acagactgtc
     6781 tttttcgtca caaatgatgt aattttttca acaatatctt gatatttggt tccag
//
LOCUS       DAYYGS010000111         6780 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000111 DAYYGS010000000
VERSION     DAYYGS010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.114"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(106..564)
                     /locus_tag="ACHA44_002520"
     CDS             complement(106..564)
                     /locus_tag="ACHA44_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8905763.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(577..1908)
                     /locus_tag="ACHA44_002521"
     CDS             complement(577..1908)
                     /locus_tag="ACHA44_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HFM8905764.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(1919..3361)
                     /locus_tag="ACHA44_002522"
     CDS             complement(1919..3361)
                     /locus_tag="ACHA44_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8905765.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(3348..4112)
                     /locus_tag="ACHA44_002523"
     CDS             complement(3348..4112)
                     /locus_tag="ACHA44_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HFM8905766.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(4216..5091)
                     /locus_tag="ACHA44_002524"
     CDS             complement(4216..5091)
                     /locus_tag="ACHA44_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8905767.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(5346..5430)
                     /locus_tag="ACHA44_002525"
     tRNA            complement(5346..5430)
                     /locus_tag="ACHA44_002525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5394..5396),aa:Leu,seq:caa)
     gene            complement(5448..5519)
                     /locus_tag="ACHA44_002526"
     tRNA            complement(5448..5519)
                     /locus_tag="ACHA44_002526"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5485..5487),aa:Gln,seq:ttg)
     gene            complement(5529..5601)
                     /locus_tag="ACHA44_002527"
     tRNA            complement(5529..5601)
                     /locus_tag="ACHA44_002527"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5566..5568),aa:His,seq:gtg)
     gene            complement(5663..5747)
                     /locus_tag="ACHA44_002528"
     tRNA            complement(5663..5747)
                     /locus_tag="ACHA44_002528"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5711..5713),aa:Leu,seq:caa)
     gene            complement(5768..5838)
                     /locus_tag="ACHA44_002529"
     tRNA            complement(5768..5838)
                     /locus_tag="ACHA44_002529"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5804..5806),aa:Cys,seq:gca)
     gene            complement(5884..5955)
                     /locus_tag="ACHA44_002530"
     tRNA            complement(5884..5955)
                     /locus_tag="ACHA44_002530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5921..5923),aa:Gln,seq:ttg)
     gene            complement(5967..6039)
                     /locus_tag="ACHA44_002531"
     tRNA            complement(5967..6039)
                     /locus_tag="ACHA44_002531"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6004..6006),aa:His,seq:gtg)
     gene            complement(6042..6115)
                     /locus_tag="ACHA44_002532"
     tRNA            complement(6042..6115)
                     /locus_tag="ACHA44_002532"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6081..6083),aa:Trp,seq:cca)
     gene            complement(6122..6202)
                     /locus_tag="ACHA44_002533"
     tRNA            complement(6122..6202)
                     /locus_tag="ACHA44_002533"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6166..6168),aa:Tyr,seq:gta)
     gene            complement(6204..6279)
                     /locus_tag="ACHA44_002534"
     tRNA            complement(6204..6279)
                     /locus_tag="ACHA44_002534"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6244..6246),aa:Phe,seq:gaa)
     gene            complement(6315..6387)
                     /locus_tag="ACHA44_002535"
     tRNA            complement(6315..6387)
                     /locus_tag="ACHA44_002535"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6352..6354),aa:Asp,seq:gtc)
     gene            complement(6397..6469)
                     /locus_tag="ACHA44_002536"
     tRNA            complement(6397..6469)
                     /locus_tag="ACHA44_002536"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6434..6436),aa:Val,seq:tac)
     gene            complement(6485..6556)
                     /locus_tag="ACHA44_002537"
     tRNA            complement(6485..6556)
                     /locus_tag="ACHA44_002537"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6521..6523),aa:Glu,seq:ttc)
     gene            complement(6571..6660)
                     /locus_tag="ACHA44_002538"
     tRNA            complement(6571..6660)
                     /locus_tag="ACHA44_002538"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6624..6626),aa:Ser,seq:gga)
     gene            complement(6700..6773)
                     /locus_tag="ACHA44_002539"
     tRNA            complement(6700..6773)
                     /locus_tag="ACHA44_002539"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6737..6739),aa:Asn,seq:gtt)
BASE COUNT         2048 a         1498 c         1103 g         2131 t
ORIGIN      
        1 acaaaaatca aaatgattaa atctagtgat tgaaacatat catttcaaaa aaattttgta
       61 aattcaccca taaatatcac cctatttaac ttttttcatc gtacactaaa attcaaaaca
      121 actatcttta atctttactt cttggttttg ttttaataaa tttccttcaa aaatgcctgg
      181 agaaactaca atgatcaccg tatctttttg tgcttgctcc aacttcttca aatctataat
      241 agctaacagt gtcccttctt ttacctcctg gttactttca acccgtatct gaaacggact
      301 tccttcgagt tcaactgtat ctaaacccat atggatcagt atttttctgc cagattcact
      361 ttcgatcgtt atggcatgtc gggtaggaaa tatattatcg attattcctt taactggcgc
      421 atggatatat ccatcatgat tcgttatagc gtatccaact cctatcattt ttgaagaaaa
      481 gacctcgtct tttactgctg ttaatggaat aatttgacca ctagctacag cttttagaat
      541 gtttcttgtt ttaaagaaat gcacttttta tcacctctac tttaatgctg gccaatattt
      601 tttgtttact ttgatcattt catctagtat tttttttgcg actctggcat ttggtacggt
      661 ttgattcaat gtaaatgctt gtaaagcttt ttgataagaa ccttcaaagt aagcatctac
      721 tagtaacttc tctactgcaa cttgtgcctc catcaatcct ttatgaaaat cactgattgg
      781 ttttcttagt gtaattggct ccacaccagt tgcaccaaca tatgccggta tttcaacaac
      841 tgcatctgac cgtagattcg gaatcgcccc ttgattagga acgattagca taaagcgacg
      901 acgatcatca ttcaacaagg aaatagctat atcgacgata tattgtccat gctccccaaa
      961 attaaagttc aatacttctt ccacaatacc gcggcgtatt ttatcagcca tttctttggt
     1021 atttttctct cgtccatcca tgaccatatt agcacgtgta tactctttgt cagtatgctc
     1081 aacgaccata tcaggatata agtaatactc taagtaatta ttcgggatat tacttggaaa
     1141 atttttcgtg atgactgaca tcatttgaaa ggttttttgc caagttttat ctcctatatt
     1201 aaaatcggcg acttgcattt cttttgttat tagtttttca ataatctctg gcataatttc
     1261 tcgttgcaat tctttatcat aaatcgatgt ataccagcca aagtggttta aaccataata
     1321 cgtaacgatc caattcttac gatcataacc gtaattcact gcaatcgttt cttcaattga
     1381 aatagtcata tcacaggcat taatcatttt tacattcgga tatttacgac gaacagcttc
     1441 agaaacgatc gactctggat ttgtataatt caaaatccat gctttggggg catagtcttg
     1501 tatatgatcg acaatttcca ataaaccttt gattgagcgc atcccataag caaatcctcc
     1561 gagtccacac gtttcttgtc ctactaatcc atgtttaagt ggaatctttt cgtccttttc
     1621 tcgcatctcc aatcctccaa cacgaatttg tgagaaaaca aaatcgcatc ctgtaaaagc
     1681 aagctttgga tcatccgttg cacgaatgcg aatatttgaa aaaccttttc ttttcagcat
     1741 aaatttaaca atcaagtaca tatccatatt tctttcttca tcaatatcat ataaacgtat
     1801 ttcagataat ggcaaccgtt tttcattgtt caaaatggct tgaacgattc ctggtgtata
     1861 cgtactcccg cctccagcta tagtgataat ctgttttttc atcatcatct tttcctttct
     1921 attcttctaa taatttattg aattctgcca taacataagt cacttctaag ccgatgataa
     1981 tttgataatt tttttcagtt ggtttaatga tcccgcttat ccctaattgc ttgattcgct
     2041 gttcctcgac catggtggga tccactacct ctacccgtag gcgtgtatta caactataga
     2101 catccaggat attggctttt cctccaaacg cttgtagcat ttttgttgcc atatattcat
     2161 aatttttact tgctaaccct aactctgttt cttctaaggt tacttcattg ccaaactctg
     2221 ccttagcttc acgtcctaat gtctgaatgt ttctcttatt gatcaaataa taaaaagtaa
     2281 cgtaatacaa ggcaaaaaag aaaattccga ttggaagaat caaaatagca ttggtagcga
     2341 ttcgaaaatt gatgagataa tcaacaatac atgctccgaa tgtaaaacca attcgtatat
     2401 tgaataagta acagacaatc cctgctaaac cggcaaaaag tgcatggatc acgtacaaca
     2461 taggagaaag aaacatgaaa ctaaattcaa caggctcagt gatatttgca ataaatgaag
     2521 ttcctgcacc gcttcccatg attcctttga ccatctcttt attttcttta taagctgctt
     2581 tatagatagc tagacatata gctggtatac caaacatcat tactacaaaa aaaactgata
     2641 aaaacagtcc agcctgagga tctccgttga taaagcgtgt catctctcct ctgaaaactt
     2701 ccttgctagg agatgtatag cttcctaaat cgtaataaat atacgtattc aacacatggt
     2761 gcagtccaaa ggggatcagc aaacgattca aaaacataaa tatccccaca cctaatgctc
     2821 ccatatcaac gagcagtttt gcaaaattat caatcccaat ttggatgagc ggccagataa
     2881 agcccaaaaa aacagctgcg attgtttgta gaatcatcac catagtcaat gggaattttt
     2941 cgcccgcaaa ataagaaaaa actactggta atttttgttc tttaaagcga ttatacgtcc
     3001 atgccgctac taaaccggaa ataattccac caaaaatccc catattcact tctgggtcca
     3061 taatttctaa gccttttttc aaggtagcaa tcgctaaaaa accagtaagt gcaggtattc
     3121 ctttgtcctt atttttgaca aaaccgattg caactcctat agcaaataag atatccaagt
     3181 ttccaaaaac ggcatttcca gctgcaccaa ttactgggat gttcagcata tcgtcagctg
     3241 ataaacgcat gatcaatccc gctaatggca tcgctgcaac tggaagtagc atcgctcttc
     3301 caagcttctg cacatttgat ttaacattca tttttattcc tcctctatta aatatcctca
     3361 tatgttacta actcaatcaa aggatcattt ttcacttttt gagcaaatgc tgaatgaatt
     3421 aaaaattcga cttggtgggt acgaggatac acaaaacttg aaccatttaa caattcctga
     3481 tccaaatacg ctgcgtgaca catgacttcg atcgttttat cttctctcaa tatcatgagt
     3541 aaacttgcca aatattcttc cacactcgta ttgatttgtc gttttttttc atcagtttct
     3601 cctacgacat cgaaacaagg ctcaaaataa tcgacatgac gaacttcatc ttttcgttca
     3661 aagttgctcc gaaccggcaa atcatatttt tttgctaatg taacaactac ctcctgattt
     3721 tcatccaaag tatgagtatg atgatgactg tctaaatgag ttggtatgat ccccgcatga
     3781 taaatttttt gaatctgagc attccattcc tgatacagtt gatctttgtc aatgttaaat
     3841 ggatgctgat aataagataa ggatctgaac tctccgcttt cgataagagt atcgacagtt
     3901 ttgaggagtg gtttgccaca cgtcaatgtt aaatggactc ctacacctag cctaggcatt
     3961 tcctttctta gtttcacagc atgctcgaaa cccggcatat ttgccatcaa tgtagttgat
     4021 gtcaaaattc ctcgctgata agcatccata atcccataat tcacaccatg gctatagcca
     4081 aaatcatccg aattaaaaat cacttttccc atttcattct ccttcttttt tctactaaaa
     4141 taggcgcaac caaataatca tagtccattt actatgtgtc atttggttgc gcctatagat
     4201 agttacgccc ttattttaat agattcttgc tagatgaatt gccaaataga tgatttcatt
     4261 ttcttgaatg gcaagttgaa attgctgttc gatgatatct ttaatcttta gtgctgtaca
     4321 atacgctttg ctatgtttaa atgtttcagt aaaaaaacca atattttcta ctggcgtttc
     4381 ttcctgataa atcaagcgtt taaccaggaa tttgatatga accattaagc gatgataagt
     4441 cagtgattct ttatcataaa cgtttgaata ggatttttct agatgggaca aaatcgtggt
     4501 cgtcatttct actattttta agctctttaa actattaggc tctcctagct cagcattaat
     4561 gatatgcata gtgatgaatc ctacttcatt tgaccctaac tcggtttgat atcgatgatt
     4621 gattcgctcg accacttcta ctgctatatc atattcattt ggataaagga atctcatttc
     4681 tggcaacata attgctgtga catctctatc ttctcttttt cgttgaatag caaaataaat
     4741 atgatctgtc aatgtaatat agatatttga atctaatgga gaggagatcc tagctgttat
     4801 cagttcaatc acttcttccg caaaatccat aaattcgatt ggaatctgcg agactatctc
     4861 actgaatcgt ttgatctttt ctttagaatc taatacaaat aatttgtcag catcctcaac
     4921 atcaaatggc ttactcactt tagcagaaaa gccaatccct ttccccatcg caattttttc
     4981 tattccatcg tcatcgatca atacagcatt atgattcagc tttttcatca catacaccgt
     5041 taattcacct cctctatatg ataaaacaaa acaacttgca gactccttca tacatatttt
     5101 tgtctccaaa caatttttaa agacaaaaaa cgacctaaga aagaaccctt tcttaggtcg
     5161 tgcctaattg aatagtagca ccctacataa tttacaaaaa tagtttaaca cataataaaa
     5221 gcgttatcaa tatctttttt actcgtttta tgataatact tgtatcgaat tgatttggag
     5281 gtaattacat aaaaacaaaa aaaatatagc aactctctat aagaaagttg ctatattgat
     5341 aattataccg gcggccgggg tcgaaccggc acgtcctcaa cggacactgg attttgagtc
     5401 cagcgcgtct gccaattccg ccacgccggc aaatttaaaa ataataactg gggtagctgg
     5461 attcgaacca acgcatgagg gagtcaaagt cccttgcctt accgcttggc tataccccaa
     5521 gaataaaggg cgaataatgg gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt
     5581 taaccacttc gccacattcg ccatcataaa agatgttatt taatttctca ataaaaaact
     5641 aagatgaaga catcttagtc aataccggcg gccggggtcg aaccggcacg ccctcaacgg
     5701 gcactggatt ttgagtccag cgcgtctgcc aattccgcca cgccggcaag ttgatttatt
     5761 agtactaagg cggtaaccgg atttgaaccg gtgatgaagg ttttgcagac ctctgcctta
     5821 ccacttggct ataccgccat tatactatta tcaatctata aaataaagaa taaattatag
     5881 aaactggggt agctggattc gaaccaacgc atgagggagt caaagtccct tgccttaccg
     5941 cttggctata ccccaagaat aaaaagggcg aatgatggga atcgaaccca cgaatgccgg
     6001 agccacaatc cggtgcgtta accacttcgc cacattcgcc atggcagggg cagcaggaat
     6061 tgaacccaca ctaacggttt tggagaccgc tgttctacct ttaaactatg cccctataaa
     6121 atggaggaga gtggattcga accactgaac cctaaggaac ggatttacag tccgtcgcgt
     6181 ttagccactt cgctactcct ccatggtggc gcgggacaga atcgaactgc cgacacacgg
     6241 agcttcaatc cgttgctcta ccaactgagc taccgcgcca tattttaaaa ttattaattg
     6301 aataacaaga acgacggttc cgacgggaat cgaacccgcg atctcctgcg tgacaggcag
     6361 gcatgttaac ccctacacca cggaaccagt tcattatgga ggttaacggg atcgaaccgc
     6421 tgaccctctg cttgtaaggc agatgctctc ccagctgagc taaacctcca acattcaatt
     6481 aaaatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc
     6541 gcttgaccaa cgggccaggt aaatttgcta cggagagtaa gggattcgaa cccttgagac
     6601 agtttggcac catctacatg atttccaatc atgctccttc ggcctctcgg acaactctcc
     6661 agagtgaaag aagctcaata ctctttctca aaatgagaac tccggcagta ggactcgaac
     6721 ctacgacatc atgattaaca gtcatgcgct actaccaact gagctatgcc ggaataacat
//
LOCUS       DAYYGS010000112         6773 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000112 DAYYGS010000000
VERSION     DAYYGS010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.115"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            125..352
                     /locus_tag="ACHA44_002540"
     CDS             125..352
                     /locus_tag="ACHA44_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFM8905768.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            368..1534
                     /gene="hemW"
                     /locus_tag="ACHA44_002541"
     CDS             368..1534
                     /gene="hemW"
                     /locus_tag="ACHA44_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFM8905769.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            1769..2812
                     /gene="hrcA"
                     /locus_tag="ACHA44_002542"
     CDS             1769..2812
                     /gene="hrcA"
                     /locus_tag="ACHA44_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HFM8905770.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            2906..3469
                     /gene="grpE"
                     /locus_tag="ACHA44_002543"
     CDS             2906..3469
                     /gene="grpE"
                     /locus_tag="ACHA44_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFM8905771.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            3520..5349
                     /gene="dnaK"
                     /locus_tag="ACHA44_002544"
     CDS             3520..5349
                     /gene="dnaK"
                     /locus_tag="ACHA44_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFM8905772.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            5500..6666
                     /gene="dnaJ"
                     /locus_tag="ACHA44_002545"
     CDS             5500..6666
                     /gene="dnaJ"
                     /locus_tag="ACHA44_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFM8905773.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         2314 a         1172 c         1524 g         1763 t
ORIGIN      
        1 aaaaaatcat ccaagaagtc gattcttgga ttttttagtg gatttgttcg ccaaatacgt
       61 ttagtatgcg tatatgagag gttgtaagca aagcgttgtg gtctgagcac ttaaggagga
      121 atctgtgaaa atagcttttc atatttttgc agattttgac ttaatgccga aggtcagctt
      181 tgcgattacc gtttatcgag aggctgtaaa agcaccgtgt agctccgatg acaataagca
      241 agcattttta gagctaggtt tccttgattc taaatatgcg gcttatttca atgagttggt
      301 gcttgaacgc gattttaaga aggtcgaaaa caggcacgac ttttcgacgt aaggagaaag
      361 aatttcaatg atagaaacaa taaatcccgc agaaaacaag gtttctgcgt acattcatat
      421 cccattttgc gagcatatct gctattactg tgactttaat aaagtttttt tagaagggca
      481 gccagtagat gagtatgtgg aaatgctgct gaatgaaatg agattaatgc tggaaagtca
      541 tcctacagcg aatttggaaa cactttatgt aggaggcgga acaccaactt ctttatctgc
      601 taaacaactt gatcgactat tatcaggtgc tagagagatc ctgccttttc aaaatggtaa
      661 tgagtttact gtagaagcga atcctggtga tttgacccgt gaaaaacttc aagtgatgaa
      721 aaactatggc gtcaatcgtt tatccatggg agtgcagaca tttgacaatc gcttattgaa
      781 aaaaattgga cgtaaacata ctgcagaaga tgtgtatcaa acaatgcaat ttttagaagc
      841 agaaaatttt accaatgtaa gcatcgattt gatttatgca ttaccgggac aaacactaga
      901 aggatatcgg gatactttgg aaaaagcttt ggctttggat ctgcctcact attctttgta
      961 ctcattgatc ctcgaaaaca aaacaatgtt catgaactgg gtacgacaag gaagactcga
     1021 actgcctgat caggaaacgg aaaccagaat gtttgaagag acgattgaag caatggaaaa
     1081 gcatggacgt caccaatatg aaataagtaa ctttgggctc tccggacatg aatctcaaca
     1141 taacctcatg tactggaaca atgaccacta ttttgggttt ggagctggtg ctagcggtta
     1201 tcttggagat attcgttacc gcaacaaagg accaatccaa cactatttac gcccattacg
     1261 agcaaatgaa cgcccggtat tggaagaaga aaaattgaca cgaaaaaatc aaatagaaga
     1321 agaaatgttc ttaggactaa gaaaaaaaac agggattact aaaaaacatt tctatgatcg
     1381 ctatcaaaaa acaattgaat cggtgtacgg tgatgtgcta agggaattag agcaagaagg
     1441 gttacttgtt aatgaagaag accgtgttta tttgacacca agagggactt ttttggggaa
     1501 caatgtgttt gagcgctttt tatttgaaaa atagtttgaa gaatatttgt tttttatcga
     1561 aacttgaaaa gaaaaagttt agaaagtttg acaatgaggt attgaagtat tgatataact
     1621 ggatttacat caattattta gaattagcac tcttgtgttt agagtgctaa aaaatggagt
     1681 ctttctcttg acaggagaag ggcttcttgc tatatttata agtgaattag cacttagaat
     1741 atatgagtgc taatgagagg tgaggaagat gttaacaaag cgacaaaacg acattttacg
     1801 tctgatcatc caacactata cgagtagtgg cgtgcccgtt ggttccaaaa cattaatgtc
     1861 tgagggagtc aaggccagct cagcaacgat tcgcaatgat atgaaagcat tagaagatga
     1921 aggattgctg ttgaagactc actcttcttc tgggcggatt ccatctgctt taggctatcg
     1981 ttattacgtg gatcatttgt taaaacctgc tcgcgtagca gaagatgatt tgctgctgat
     2041 tcgtcagtct ctaggcagag aatttcatga aatcaatgag attattaagc aatctgctga
     2101 gatattatct gagctgacaa gctatacgac tttttcattg ggacctgaaa taaaagaccg
     2161 ccgactgact ggctttcgta tcgtaccctt gaataatcga cagattattg ctatcgtcgt
     2221 aacagatatg gggaatgtag aaagccaagt gttcactctt cccgaatata tgggaagtca
     2281 ggaactagaa aaaatggtac agattgtaaa cgaccgtcta gtcggagatc ctctgatgac
     2341 ggtgtaccat aaacttcgta cggagattcc gatgattctt cataaatatt tccagacgac
     2401 agaagggatg tctcagttat ttgacaccgt tcttggtcat gtttttgaag aaaaggttta
     2461 tgtcagtgga caaatgaatc tcttggatta tgagccccgt caagatcttg atcagttcaa
     2521 atcgatgttt tcttttatga aagactcaga cggacttaca caaatgatca ctccgatgga
     2581 tagcaatatt catatcaaga ttggtgctga attaggcaat gatttattgc agaatatgag
     2641 tatgattcaa gcaagttacg agatagatgg tcatggaaga ggtacaatag ccttgttagg
     2701 gcctactagt atgccttatt ctcggatgtt cggtctagtc gatgtttatc gaagagaact
     2761 agcagctaaa cttgctgatt actaccgctc gcttgacttg tcaaactcgt aatgtgaata
     2821 acgaagctat ttgcagctga tcgttctctc tttcttagta agagcaatca aacagctgac
     2881 tgaataatga aaggaagact gtatcgtgaa agaaaatcaa gaagaactag ataaagaaat
     2941 gacagatgct cagccagaac cggaaatcga tgtcgaagct gcagaagatt cgggtatttc
     3001 agaagctgaa gcggaagaat tcgaaacagc gaaattaaaa gctgagttag aagaaatgga
     3061 agataaatac ttgcgggctc gtgctgaaat cgcaaatatg gcaaatcgag gaaaaaatga
     3121 gcgtgagcaa ttacaaaaat accgttcaca agatttagcc aaaaaattac ttccatcaat
     3181 tgacaacctg gaacgtgcat tagccacaga agtgtcagat gatcaagggg caggtttgaa
     3241 aaaaggcgtg gaaatggtct tggaaagttt gcgtaacgca ctggaagaag aaggaattga
     3301 aaaaatccct gctaaaggag aagcctttga tcctaatctt catcaagcag tacaaactgt
     3361 tccagcgact gaagatacac cagccgatac gattgtagaa gtactacaag aaggctacaa
     3421 attacatgat cgcgtgttaa gaccaactat ggtcattgtt gcacaataat tgtgcggata
     3481 taaaacttac actttcaatt aaagaaggag acgttaatta tgagtaaaat tatcggtatt
     3541 gacttaggta cgactaactc tgcagtagca gtattggaag gaaacgaagc aaaaatcatc
     3601 gctaacccag aaggaaaccg tacaacacct tctgttgtat cattcaaaaa tggagaaatc
     3661 caagtagggg aagttgcaaa acgtcaagct gtgacaaacc caaatacgat ttcatcaatc
     3721 aaacgccata tcggtgaagc aggttacaaa gtagatgtag aaggtaaatc atatacacca
     3781 caagaaatct cagcaatgat ccttcaatat atcaaaggat tcgcagaaga ctatttaggt
     3841 gaaaaagtcg aaaaagcagt tatcactgtg ccagcttact ttaatgatgc acaacgtcaa
     3901 gcaacaaaag atgctggtaa gatcgctggt ctagaagtag aacgtatcgt caatgaacca
     3961 actgcagctg ctcttgccta tggtttagat aaaacagatc gcgacgaaaa aatcttagta
     4021 ttcgaccttg gtggcggtac ttttgacgta tctatccttg aactaggaga cggtgtcttt
     4081 gatgtattgt caacagctgg cgacaaccat ctaggtgggg acgactttga taacaaaatc
     4141 atcgactaca tggtagcaga attcaaaaaa gaaaacagca ttgacttgtc tcaagacaaa
     4201 atggctttac aacgcttgaa agacgctgct gaaaaagcga aaaaagattt atctggcgta
     4261 tcaagcacac aaatcagctt gccatttatc acagcaggag aatctggacc attgcatttg
     4321 gaaatgacat tgacacgtgc aaaatttgat gaattaacag ctgacttagt agaacgtaca
     4381 aaagtaccag tacgtcaagc gttaaaagat gcaggtctgt cacaatcaga aatcgacgaa
     4441 gttattttag tcggtggatc tactcgtatc cctgcagttg tagaagcagt acgtaaagaa
     4501 acaggaaaag aaccaaacaa atcagttaac cctgatgaag tagttgctat gggtgctgct
     4561 atccaaggtg gcgtgatcac tggtgatgtg aaagatgttg ttttacttga cgttacacca
     4621 ttgtcattag gtatcgaaac aatgggtgga gtgttcacta aattgattga ccgcaataca
     4681 acgattccaa caagtaaatc acaagtattc tcaactgcgg cagataatca accagcagta
     4741 gacatccatg tattacaagg tgaacgccca atggctgctg acaacaaaac tctaggaaga
     4801 ttccaattga cagatattcc agcagctcct cgtggtattc cacaaatcga agtaacattc
     4861 gatattgata aaaacgggat tgtaaatgta tctgcgaaag atctaggtac acaaaaagaa
     4921 caaaaaatca cgatcaaatc ttcttcaggt ctaacagacg aagaaatcga acgtatggtg
     4981 aaagatgcag aagcaaacgc tgaagcagat aaagctcgta aagaggaagt tgacctacgt
     5041 aatgatgtag acgcattgct attctctgtg gacaaaacat tgaaagaatt agaaggcaaa
     5101 gtggacgaag aagaagtgaa aaaagcagaa acagctcgtg acgaattaaa agccgctgtt
     5161 gaagcaaaca acatcgagga aatgaaaact aaacgcgatg cattgaacga aatcgttcaa
     5221 aacttgactg ttaaactata cgaacaagct gcacaacaac aagctcaaga aaatccagaa
     5281 gcagctcaag gcggagcaga tgatgtcgtt gatgctgatt ttgaagaagt cgatggcgac
     5341 gataaataat cagttcactg aatttcgaga gaaggccaaa gttgctactt tggctttttc
     5401 ttttgaaaaa tagtttgaag tatcggtttt tgtttcaaac aaaatatgat atgataacaa
     5461 gcgacgtgaa aatgatgaga accaatagga ggaaagccta tggcaacaaa acgtgattat
     5521 tatgaagtct tagggctctc aaaaggagca tcagaagatg aaataaagaa agcttatcgt
     5581 aagctttcga aaaaatacca tcctgacatc aataaagaac ctgatgcgga agaaaaattc
     5641 aaagaagtat cagaagcata tgagatttta agtgacccgc aaaaacgcgc tgcatacgac
     5701 caatacgggc atgcaggcac agatccaaat tacggtgctg gtggagctgg tggcggattt
     5761 ggtggattcg gcggcttctc tggtggcggc ttcggtggtt ttgaagatat cttcgactct
     5821 ttctttggag gaggcggtcg gtcagtcgat ccaaatgcgc ctcgtcaagg tgctgatttg
     5881 caatatacgg tcgacttgtc ttttgaagaa gcgatttttg gtgtagaaaa agaaatcaag
     5941 tataatcggg aagaaatctg tcatacttgt ggtggaaacg gtgcaaaacc aggcacacaa
     6001 ccaactactt gtcataaatg tcatggtgca ggtacgatca atgtagaacg ccaaacgcca
     6061 cttggacgag tgatgagccg tcaaacttgt gatgtttgtc atggcacagg gaaagaaatc
     6121 aaagaacctt gtccaacctg tcatggctca ggacatgaga agaaagcaca ttcagttaaa
     6181 gtgaatgtac cagctggtgt agaagaagga caacaaatgc gtttagctgg tcaaggagaa
     6241 gctggagaaa acggcggacc atttggtgat ttgtatgtgg tttttcgtgt agaagaaagt
     6301 gatatctttg atcgtgaagg ttctgaaatt tactatgaat tgcctttaag ttttgtacaa
     6361 gcagctttgg gggatgaagt ccaagtacca actgtccatg gagatgtgaa attgaagatc
     6421 cctgctggaa cacagacagg tacgaacttc cgtttacgtg gaaaaggcgc tccgaagtta
     6481 cgtggtggtg caacaggcga ccaacatgtc aaagtaaaac tgatcacacc gaaaaatcta
     6541 aatgaacaac aaagagaagc attacgtgca ttcgctgaag ctggcggcat caaagttgaa
     6601 gaacagcaag aagacggttt cttcgataag tttaaagatg cttttggcgg gaaaaagcgg
     6661 aagtagtttt tctagtaaaa atataaagta gacttgccca aattttctat ttctagtaaa
     6721 aacgctaagt cattaataat taaaaaccag acgaacaatc atacgttcgc ctg
//
LOCUS       DAYYGS010000113         6759 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000113 DAYYGS010000000
VERSION     DAYYGS010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.116"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(194..1105)
                     /locus_tag="ACHA44_002546"
     CDS             complement(194..1105)
                     /locus_tag="ACHA44_002546"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8905774.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(1562..1990)
                     /locus_tag="ACHA44_002547"
     CDS             complement(1562..1990)
                     /locus_tag="ACHA44_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905775.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(2018..2656)
                     /locus_tag="ACHA44_002548"
     CDS             complement(2018..2656)
                     /locus_tag="ACHA44_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFM8905776.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(2660..4696)
                     /locus_tag="ACHA44_002549"
     CDS             complement(2660..4696)
                     /locus_tag="ACHA44_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HFM8905777.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4822..5487
                     /locus_tag="ACHA44_002550"
     CDS             4822..5487
                     /locus_tag="ACHA44_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905778.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
BASE COUNT         2178 a         1231 c         1021 g         2329 t
ORIGIN      
        1 ttgatattgt tttctttatt taagattata cagaaaaata tctagggtcg ctggattcta
       61 agtaaaaaaa atttttaaaa taaaaagaac taaatcaagt agtaattaaa ctactgttga
      121 tttagttcta ataaaaagat ttagatgaac agttttattg atttaaatag ttcatatttt
      181 ttatttatac cgcttattta ctacttgctg tactagaatc tgaagtgaat gtactcaaaa
      241 cattttcaaa gtctttgtct ttgattttta cattagcgtc tttcaatact tcgccaatta
      301 ctttattctg gaaggtactg tcacttaatt ttgtgtctgt tgcaatttct ttcaattctt
      361 ttttgtattt atccatgtcg ttgcctttgt tttggttttt caccatttta accacataat
      421 attccgtagt ataagttgaa gcattggtag aagtgatcac atcagaaact tgaccatctt
      481 tcaacttaaa ggctgcttct ttgacttctg ccggaaccgt tgtagtagtg gaatcaaatt
      541 tcacttttcc gccatcttct tttgtcgtag tatcagttga cttgtctttg gctaatttag
      601 agaaatcgtc tcccttatca gcagctttct tcacatcttt tgcatcatct tcacttgcta
      661 cttggataat ttgagcttcc acttctggat ggaaagattc ccaagccgtt tttaaatctt
      721 catcggtcaa tttgatatgt ttctttagac cttcttgaaa ggctagactt tgtttgatac
      781 tgtctttgaa tgtttgttct gtgtaacctg cagatttcaa ttgtgaatca aatgaatcac
      841 ccaattgttt ctttgtttga tcatattgag aatcgatttc tttggttgat acgtcatctc
      901 catatttatc ttcaaatact tgataaacga tcaactgttg cacagcggaa tctgctcctt
      961 gcattttttt caagctatcc attacttctt gttcgctgat tgtaccgcct ttataactaa
     1021 cggctgtttt ttcaccggca ttcgtacagc ctgacaataa gccagtgcct agtacgatgg
     1081 caagtgctaa aaatcttttt ttcatttatc aattctccta cttttcgttt tttgcgaatt
     1141 gctaaaaaca aaggtgttca agatgaaaat catcaagaac acctttacta atctgctcga
     1201 tacgtcctga atagatataa ggctacacaa tccagaaagt tcgctcagca gaataaatgt
     1261 caagtagcac atcacacctt aatatactac ttcttttgtt accgtttttc aagtctatct
     1321 tgcgttccgt atttaacgta actaattctt agaacaaatg ttatcgattt tgtttttctt
     1381 cttccgctgc tgggatattc aaatcagaca cttttatgtt cgattttcca gacatttttt
     1441 tgttgttttt attatgatat atttttatac aactaaaaat gaatatatta aaactaccaa
     1501 aaataacttt taacgctgtt tcattatatg ttttttctat tttatataaa cttttggata
     1561 cttaatacca accggttgga ttgtaccaac atgagaatgt gtcagttcta tttcctgtcg
     1621 cacttgcata ggcccatact ccttttgttg catttgctga tcgaatagag tttccaactt
     1681 tcgcttttgc accgtgacga ttatagacac gactatagtg gttagaatga cctgtttttc
     1741 tcgtacaagt tgtatcacta taataaaccc atcttccaga gctatgatta attgctgttc
     1801 tagaaatttc ccggtctact ggtaaatttg acttaataat attaccttta gcatcaaaat
     1861 gaatctctcc tggctttaaa gatttgtaat agatttcacc gtgaactgaa tttgcttcca
     1921 cttcagatga catagcgttt cctccaacaa aacctatccc tagtgcacaa ataataactg
     1981 cttttttcat aaaacgacat cctttccttt ttgattttta aagcatgatc ttcttgtttc
     2041 ctattttggt cagatatgga tcatgcgtga ctacgataac taacgtacct tgggaagctt
     2101 gttgcgataa gatttcaact actttatcct tatttctttt atccaatgac ccagtcggtt
     2161 catcggctaa aatgattttg ctattcttca ataagactct cgcacaggcg attctttgct
     2221 tctcaccacc agataatgtg tagataggcg tacttagctt cttgtttata tttacttttt
     2281 tcaatgcctc atactttgct gttcgcttat ctgaaacatt taaattcgaa aacttcaatc
     2341 caatatctaa attaaaatcg attgtttcat attcaatcaa cccaaaatct tgaaaaagaa
     2401 aagatatggt atttcttttt atcaagcgct tctctctccc agttaattgt ttcgtctctt
     2461 gatcgaacaa atagaattgt cctgaactga actgatcatt caatcctaat atattcaata
     2521 acgtactttt acctgctcca ctttctccaa caacagtcac aagatcccca gcatccgcag
     2581 tgaaacgatc caaatgaaga atcgttttgt ttcgaatgat ttttttcgca ccttttatca
     2641 ctatcaacgg ctccaattgt taacctccat tcatcagcaa taactgactt ttcttttctg
     2701 tagacaaaat aaaaacaact gaaagaataa aatcaatgaa gctaaagaaa aaaatattaa
     2761 aaagatattt aggctctctt tctataagaa aaaggaagat tacacttgtc gttccccatt
     2821 tgatgaggtt catcatgaag aaatctttat acgtttcaaa gagcgaataa ccacttgaac
     2881 gaaagatagc gattttccga caattgtaga caaaatatat gcataacatg acatagctca
     2941 acaagaagaa agaaaagaaa gtgagtaaaa atatctgtat caagtaatga aatgatctgc
     3001 ttaacacttt gacagctatc tcttcagtct gagtatacgg aaatgataac tgcaagttat
     3061 cacgtaactg actttcttca atcagctcaa ataacctttc tttcgacaaa gagcctatct
     3121 tgatctttaa cggtggatag atttctccgc tgagaatatt tctttccaaa tacatactgt
     3181 ttttcaatgt cagtatttca acaaccggat aatgttcgat ccaggggata gaaggaataa
     3241 atgtataaat gggttgattg ttttttataa atagaaattt cggttctttt tcactgattt
     3301 cttgataata ttctgttatt tgcgccaggt cattttttaa atctgtatgt ctatatcgtt
     3361 cagggatcaa tataacaggg tgcgtttctt tttcggttat ttggatcaac cttccagaga
     3421 catcaatcaa aggaaatttc ttaagataat tcagatttat ttttatccct ctgccaaact
     3481 gttcattttc ttcattcaag taatctgata cattgactaa tatacttccg tttttattca
     3541 aagtttcata taattcctga tctgctgctg atgcaaaacg attggaataa ataaaatcca
     3601 cgtggttttt cccgatggtt actggataaa atacatgata gtcctcacca gcgatttctt
     3661 ttggtggttc atgggaaaca gctaactgtg aagaagaaaa aacgatagtt gctaattcga
     3721 cattggcaac ggtcaaaaga agagaaaaca acaatccaaa tgtaaaaaga atgtattgga
     3781 tatagttgaa cgcttctctt aacgaagcga ttttttcaac taataaaaga gaaatcaaag
     3841 agataccata tattccgctg aacaagaaaa cgtacatctg gaacaaataa agaaattgct
     3901 ccttataata cataaaaata aaaccaccaa tgattcctgc aattatgaat gtaataatga
     3961 ttttcttttt ccccaataaa caccacaata cttgatgtaa tgaaaagtca ttattcaaca
     4021 ataaactgat ttctttattc atcaataaga aaaaggatga taacaaaagg aataaaagaa
     4081 tcaatccaat agttaaatac actcgataat tatatacgtc tgtatttgaa ataaccgaat
     4141 acgaatagct attttcaaag tcttcaaaaa tattagtggc ataatcagtg tgaaatgctt
     4201 gattgaatcg atcagtgaat agtgaaatga accgttcata ggcttctttt tctctcgtca
     4261 tcaagaaaaa ttcaccttca aaatctttta ttttgatcga ttcttcaatg gacgttacgc
     4321 gaaaggaggt attagaatag ggtaccttct cgtgagaaga tttgacatcc ccttgataat
     4381 acaatgtcat ttcaagcttt ggctgtatct tgaagttcca agatgtatct acttcattgg
     4441 attcatttac tgtttttttc atataagaaa gctcagtttc tgatgagaca ttttcaaata
     4501 ccatgattag ctgctcatat tcttcttgcc caataaaatg ttgtggcaat cgaatcggag
     4561 tataagtatc tgtttgatgg aaaaattcat ttgtccgata gagtacctgt ttatttatac
     4621 taaccaaggc aaaaccactc atgataccgg tcactatgat tgtgagtaaa actgtgaact
     4681 tttttttgat tatcaccact ctcccttact taaatgattc tttgataaaa tcataccgtt
     4741 tttcatcaaa aaaacgtatc tatcaagtgt ataggtcatg tagaataaat ctatatactg
     4801 tagaatcgaa aggagaagaa gatgaacacc ggagaaaaaa tacgaactta ccgcagaaat
     4861 tgtaagctta cgcaaaaaga attaggcgaa aaaattggtg tttccgataa aacgatttct
     4921 agttgggaaa attctcgtac aatgccagac cttgaaatgc tgtcactttt acatcatact
     4981 ttaggtctgc cgattgattt tccttctatt gaatcagcta ccgaatcaat agaaccaaca
     5041 ttgccagatg aacctcttgt taacaagaaa tcttattcat caaaacaacc cttctcactc
     5101 aaaaagacac tatctttctt tctgctcgtc ttttgtttgg tctatctttt tcttatcaat
     5161 tatcaataca aaaatgaaac gatcgatcga ttcaatcctt ttttgaaacc acaaatcggc
     5221 tataccacac tccctgtagg atacgtggac aattccatcg aacgttgggt atgtaatgat
     5281 atctttggcc atggtacatg gctgtctttt cacgctggag aggtagaaaa agagaaaaac
     5341 catgctgtaa tcaaacacaa aggaaaacac gtcatgaacg ttcgcctgct cacttgggaa
     5401 gccattccct cagattacca agaaatcctc agccaagact atgacgacgc cttaaaaaga
     5461 agccctcagg agccagatag gcattaagta ggagatgaac gtagagatag aaaaaatcaa
     5521 tgcacatgat cttatccaaa aaacaggggt acaaattaat tgaaaaagcc ttgagatgaa
     5581 agttcctaaa taaaaaagtt cagaatgata ggtatttctg gcttatagta taatatttac
     5641 gccacagcct cccaatgatt ctttttggaa tcataatcaa ggatggacat tcacaaaaat
     5701 attttgctca ttcgatccga atagattaac gaccaatcta gtaagtaata tagattggta
     5761 cgtagtcaag ctgcacataa tttagatcaa ttttagttct tttttatatt tctatgatct
     5821 gctcatttac ttttatatga tggcttacag taacataaat aaatatgata atcgctggta
     5881 gtttatccaa gagtactcaa taaaatacaa aatggattgt tcaagcgcct atcataatga
     5941 taaaatgaaa caaataaacg atacaaggag gaagagactt tgagtaatgg cagaacaatt
     6001 ttttcattgt aaaaattgtt cagttgttaa tctatgggat ttcggtagca tgggcatttt
     6061 tttatagagg aatcacacca acctggccgt tttatctctt gttagtggga atattagtag
     6121 gaaattttgc ccctcttcac tatcgtacga acttttttcc aactgattct ggcctttttc
     6181 ttaaaacaaa gacgcgaaaa ttagaaaaca ttattgaaat gacaattacc tgtataatca
     6241 ttttcttagt tatattccta aacgtcttct aataagaatc tcttaaaatt agcttgttac
     6301 agttagttac ctagtttata taacacagtt atcttatctt ccttatcaac atcttttgcc
     6361 aactctttgt cttaaattcg aaacatataa gcagagctac gaacaccaag actgtttcgg
     6421 ctctgttttt tgcatcaaat tcactcttat atagctgata atctcggctc tttaaaagaa
     6481 attattttcg cttcatttta ttataaaaag gctaggaacc ttgatgttat caggttccta
     6541 gcctttattt aagtattacc ccacagaccc ttccatatag atttttaaat tacacactag
     6601 tttaaactaa aacaaacaag gaaacactta ccatttaatc ataaaattag ctcatatgaa
     6661 tatattagaa tgtaaaatag actggtcaaa atctggtcaa aaaagaggac taatacaggt
     6721 agtcctcaga ctgtagacaa agtccatttt atggatgat
//
LOCUS       DAYYGS010000114         6518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000114 DAYYGS010000000
VERSION     DAYYGS010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.117"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1093
                     /locus_tag="ACHA44_002551"
     CDS             86..1093
                     /locus_tag="ACHA44_002551"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050377 - UDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase family
                     protein"
                     /protein_id="HFM8905779.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1105..2109
                     /gene="neuB"
                     /locus_tag="ACHA44_002552"
     CDS             1105..2109
                     /gene="neuB"
                     /locus_tag="ACHA44_002552"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HFM8905780.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2106..3260
                     /gene="neuC"
                     /locus_tag="ACHA44_002553"
     CDS             2106..3260
                     /gene="neuC"
                     /locus_tag="ACHA44_002553"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFM8905781.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENENEIETALN
                     KIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            3233..3910
                     /locus_tag="ACHA44_002554"
     CDS             3233..3910
                     /locus_tag="ACHA44_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFM8905782.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            3861..5042
                     /locus_tag="ACHA44_002555"
     CDS             3861..5042
                     /locus_tag="ACHA44_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFM8905783.1"
                     /translation="MAIQLKLRKKELILNSRILLSSPHMGGTEEKYVAKAFKDNWIAP
                     LGENVDKFEESITKYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATV
                     NPILYQGAVPVFIDSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYER
                     ILAIANQFNVPVIEDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSD
                     KKYVEHSRFLATQARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIF
                     NRYKEAFSGIDRIEMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRA
                     LWKPLHLQPVFQGVKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIF
                     K"
     gene            5055..6032
                     /locus_tag="ACHA44_002556"
     CDS             5055..6032
                     /locus_tag="ACHA44_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8905784.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            6032..>6518
                     /locus_tag="ACHA44_002557"
     CDS             6032..>6518
                     /locus_tag="ACHA44_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8905785.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KS"
BASE COUNT         2432 a          814 c         1240 g         2032 t
ORIGIN      
        1 attattgata attagtttag ttatatattt tttaaagatt aagaatcgaa ttatagatga
       61 ctataattca taggaggaga tataagtgtt taaaggaaaa acaatattag taactggcgg
      121 aactggttcc attggaagtg aaattgttcg tcagctttta aaatatgaac caaaagctat
      181 cagaatttat agtagaggtg aagaaaaaca attttatatg caacaagagc tctcaaatca
      241 cactaatttg agatttttaa ttggtgatat cagagactct caaaggattg attatgcatg
      301 tcgtgatgtg gatattattt ttcatgctgc cgcaatgaaa cacgttccag caagtgaata
      361 taatcctatg gaagctgtaa aaacaaatgt gatgggaact caaaatgtaa tagatgcagc
      421 gatcaaaaat aatgtaaaac attttgtagg tatcagtaca gataaagtag tgagtcctac
      481 taacaccatg ggtgcgacaa agttattgag tgaaaaatta ataatagctg ccaatcagta
      541 taaaggaaaa acagaaacaa tattttcttg tgtaaggttt ggaaatgtca tggggtcaag
      601 tggatctgtt attcctcttt ttagtaaaca aataaaaaac aataaagata ttactttgac
      661 agatgttgat atgacacgat ttatgatgtc aataccggat gctgcaagat tagttctgtc
      721 tgctgctatg aattcaaaat ttggagaaac atatgtctta aaaatgccgg caatcaatat
      781 tttaacttta gctcaatcat taataaaaat atataatgat acacatgatg aaaaatatag
      841 aggtgaaata aaagttgtag gtatcaggcc aggagaaaaa ttatatgaag agctaatgac
      901 tacagacgaa attgaacgct catatgaaaa tgataaaatg tatatcattc caccacacta
      961 tcatgctact gacgaactgt acaaagatta tgaaaaaatt aaaatcactg catactcttc
     1021 agatgcaaca gaattaatga gtggacagga atttattaga atcgcaaaag agaataatgt
     1081 cgtgacttgg taaggagttt tatgatgaaa tcagaagtat atgtaattgc agaaattggt
     1141 gtaaaccata atggaagtct agatttagct ttagaaagta ttgataaagc taaagaatgt
     1201 ggagctaacg cggttaaatt tcaaacattt aaaacgaata agctagtttc caaaaaggct
     1261 ccaatggcaa aatatcagaa ggataatctt aaagaggaca gttctcaatt tgaaatgcta
     1321 aaaaaattag aattatctca atctgatttt attagaataa aagattattg tatacaaaat
     1381 aaaattgatt ttttgtctac tccttttgat gaagaaagtg cagactttct aaaacaaatt
     1441 ggtgtacaag gatttaaaat tggctcagga gatttaacaa atttaccttt tcttagaaaa
     1501 atagatatgt atgggttgcc agttttatta tctacaggta tggctgatat tcatgaggtc
     1561 agtgaggctg tagattgttt catagatagt ccagttacta ttttacattg tacttcaaat
     1621 tatccggcta gttctgaaga tattaattta ttggcgatta acacattaaa agaaatgttc
     1681 gggatacccg ttgggtactc cgatcattca ttgggatatg atgttggtat ttgtgctgtt
     1741 gcgatgggtg ctaaggtaat tgagaaacat tttaccttgg ataaggaact acctggtcca
     1801 gatcataaag cttctttaga tcctcaggaa tttgcagatt ttgtaaagca tattagaaac
     1861 actgaaatat ttttaggtga tggtgttaag cgagcaatgc cttctgaact tagtacaaaa
     1921 gaagtatcaa gaaaaagcct agtggtcact gagaatttaa atgtgggaga tattcttaca
     1981 gagaaatctc ttacaataaa acgtcctgga aatggaatag ctcctaagtt tttatatcaa
     2041 tatattggca aaaaagttaa aagaccaata agtgcagata gtgttctgct tgaggaagat
     2101 gttgaatgaa aaaaatactt atagtaagtg gaacacgagc tgattatgga atatataaac
     2161 ctgttgctga gaatttacta caagataatt gggatgtatc atttgttgtt tctggaatgc
     2221 atttatccca taaatatgga tttactaaag aattgatctt agctgacgga tttagaatta
     2281 gtggagaagt tcaaagtttg tttttagaga acaataaggg aaacatggct cggtctgtgg
     2341 cattagagat aaatggtttc acaaatatat ttgaacaaac tagccctgat tttgtattat
     2401 tactagggga tagaggagaa atgttggcag ctgcgatagc ttgtttgtat cttggaatca
     2461 aaacagcaca tattcacgga ggcgaagtgt ctggtactgt agatgaatca ataagacatg
     2521 ccataacgaa aatagcatct gttcattttg ctgctacgga aaaaagcaaa gagcgtttaa
     2581 taaaaatggg ggaagattct tggagagttt ttcccgtcgg agctcctaga atagatacta
     2641 ttttaaataa tagattacct agttttagag atataaaaga aaaatatcat ctaaaatttg
     2701 aaaagaaaaa atacgttttg caagtatttc atccagttgt aaccgaacta gaaaatatag
     2761 aaaaacaaat tttagctttg atcaattcaa tggaacaaca atctcaacca attatttgca
     2821 ttttacctaa tagtgatgct ggtagcgaaa ttatcagaaa aatgtaccag aaatatttaa
     2881 aaacggatat tatttatatt acgaatttag agccggagga atacctgact atattaaaag
     2941 agtgcaaatt tatgatcgga aattcttctt ctgggataat tgaagctgca tcattcaaaa
     3001 taccagtaat aaatttagga accagacaaa atggacgaga acaatctaaa aatattataa
     3061 atgcaaatct aaatgaaaat gaaaatgaaa ttgaaactgc tttgaataaa atacaagatc
     3121 ctcagtttat aaaaagcttg aaaaatgtta agaacatcta cggtgacggc aacagttcta
     3181 gaagaataac aaaaacattg aatcaaatat tgaatgatgg ggaagatatt aagtggattc
     3241 aaaaacaaat agcttattaa aatatactag tgatccatac attattattg gggctggcgg
     3301 acacgcaaaa gtcattattg aaaccttaag attagaaaat aagttaatat atggattttt
     3361 tgatgataag caaaataaaa tgcaagatct gaaatatctt gggaaaataa acgaattgaa
     3421 tagctgggcg gatagcaaaa aaaaaatcta taaatttatt gtagctattg gtgactgtgt
     3481 agtaagaaaa aagattataa aaaaattgaa tatgccaagt gagcaatatg gaatagctgt
     3541 tcatccaagt gctatagtgg caaaagaaat ttcaattggt tatggaactg ttatttttgc
     3601 gaatgctgta attaacccag atgctgtaat tggagagcat gctataataa acacaggtag
     3661 cattgttgag catgattgta gaattaataa ctatgttcat ttatctccag gtgtatgctt
     3721 agcgggtgga gtacatgtag gtgtaggaac tcaaatagga attggttctc aatgtatcca
     3781 gatgaagaaa attggatctt ggtgtatgat aggcgcaggg agcactattg ttaaggatat
     3841 tccctcacat tccttagctt atggcaatcc agctaaaatt aagaaagaag gaattaattt
     3901 tgaattctag aattttatta tcaagtcctc atatgggagg aacagaagaa aaatatgttg
     3961 ctaaggcttt taaagataat tggatagcgc cattaggtga aaatgtggat aaatttgaag
     4021 aaagtattac aaaatatact ggttctaagg gcggagcttc tgcgttaagt tcaggtacag
     4081 ctgccataca tcttgcctta agactgctta atgttggaaa aaacgatatt gtctttgctc
     4141 cttcatttac ttttatagct acagtaaatc ctattttata tcaaggggct gtaccagtgt
     4201 tcattgactc agaacctaat acatggaata tgagccctgt tgcactagag agcgcattag
     4261 aagaagctga aagaaacaac caattaccta aggcaatcat cgtagtgaat ttatatggtc
     4321 aaatggctga ttatgaacga attttagcta ttgcaaacca atttaatgtt ccagtgatag
     4381 aagatgcagc ggaatcgtta ggttctactt ataagggaat ttcgtcaggc ttatttggga
     4441 caattgggat ctattcattt aacggtaata agatcatcac aacctcaggt ggaggagctc
     4501 tagtgtctag tgataaaaaa tatgttgagc attcaagatt tttagcaaca caagcaagag
     4561 atcaagcacc gtattatcaa catagcgaaa taggttataa ttaccgtcta agcaatattt
     4621 ctgccggtat cggacgtgga caaatggaag tattgaatga tagagtagct aaacgaagag
     4681 aaattttcaa tcgatataag gaagcttttt ctggaattga tagaattgaa atgatgccag
     4741 aagtggatgg catggtgcca aatcgttggt tgtctacttt aacgatcaat gattataatt
     4801 ctattgatca agtagttaac gtaataaata aactaaatga aaaaaacatt gaaacaagag
     4861 ctttgtggaa accactacat ttgcaaccag tatttcaagg ggtaaaaatg tattaccatg
     4921 ataataatct tagtgtgtca gaaaagttat ttaatagcgg cttatgctta ccttctggaa
     4981 gtaatatgac agatagtgaa caagaatttg ttattgatga gtttaaaaaa atattcaagt
     5041 aggtgtgata ttttatgaac ataggaatta ttggtttagg ttccatgggg aaaagaagac
     5101 ttagactcat tcaagaacac tttaatgata ttttactatg tggtgttgat actaatgagg
     5161 cgagacgaaa ggaagttgaa aaggaatttg gaataccaac ttataatagt ctaacagagg
     5221 caaaaaattt gtttgaaata aattcagttt ttgtttgtac atcccctata acccatgaat
     5281 ttattataaa agaggcttta aatttagatt gtaatatctt tacggaaatc aatttattaa
     5341 atgaatatta tgatgaaata atcgaagtag ctaaaaagaa agatagatta ctttatctat
     5401 cttctacatt tttgaagaga aaagaaattc aatatattaa gtctaaagtg acgcataata
     5461 aaaaaatttc ttatcaatac catgtaggac agtatttacc cgactggcat ccttgggaag
     5521 attatcgcaa tttttttgtt tcaaacaaaa aaactaatgg gtgtagagaa atattcgcta
     5581 ttgaattacc ttggattata gatacatttg ggaaagttaa atcgtttact gtaatgaccc
     5641 agaaattatc caatcttgat attgattatt cggacagttt cactcttatt attgagcatg
     5701 aaactggtat agttggaact ttaagtgtag atgttgtttc aagagtagct aaaagaaatt
     5761 taactataat tggtgaaaag attttaatag aatgggaagg taacccggat tctctatttt
     5821 catggaatga agaaacttct agtatgacag atattcaatt gtacgatgag gttatgcatg
     5881 ataataatta ttcacatact attattgaag atgcatattt agaggagatt aaagaatttt
     5941 tcacgctttt ggaaaagaaa atggaacccc aatatacttt tgaaaaagat aagtatatta
     6001 ttaacttaat taacgacatt gaggtaaatt aatgatgaaa gtaatttttt ttgggttagg
     6061 gtcaattgga aagagacatc taaaaaatct aataagctat ggaaaaaaga attctattaa
     6121 ttttgatata acagcttata gtagcagtgg aaaaaaagag tttgaagaaa taacgtatat
     6181 tcaaaataga gacgatttgg atacacatta tgatatagct tttattacaa atccgacttt
     6241 taagcatctg gaaacactaa atgtaattgt tgaaaaagtt gattacatat ttttagaaaa
     6301 acctgctttt tctgagagta ttgatcttac tcaatataat tatgattaca aaaatattta
     6361 tgttgctgct ccactgaggt ataaaaatat catggaagtt gttcgtaata gtattcaaag
     6421 taaaaaagta tattctgtaa gagctatttg ttctacttat cttcctgatt ggagaaaagg
     6481 tactgattat agaaataatt attctgcctt taaatcta
//
LOCUS       DAYYGS010000115         6387 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000115 DAYYGS010000000
VERSION     DAYYGS010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.118"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(99..1229)
                     /locus_tag="ACHA44_002558"
                     /pseudo
     CDS             complement(99..1229)
                     /locus_tag="ACHA44_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
     misc_binding    complement(1289..1516)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1878..3080)
                     /locus_tag="ACHA44_002559"
     CDS             complement(1878..3080)
                     /locus_tag="ACHA44_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905786.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="ACHA44_002560"
     CDS             complement(3140..3343)
                     /locus_tag="ACHA44_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8905787.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4552)
                     /locus_tag="ACHA44_002561"
     CDS             complement(3485..4552)
                     /locus_tag="ACHA44_002561"
                     /inference="COORDINATES: protein motif:HMM:NF014537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8905788.1"
                     /translation="MLDSLQEEVVPVNRVLIDYLTIHFFSNQHLKLIEEIIEMPIERF
                     EELDYAPKGYIGQYVWNQVITIRYSIDDAVKGTVMEFSGQGCKHLARWLKTRKTNWQQ
                     FFRKVLDYQGNFTRIDFTLDDFVGSLSIPELKHKVILGHVWTTFQVSESHGGTDIINN
                     ESNGETLYLGSKKSQCRFCFYQKDHEQRKRRGIPLEEAKVKNRFELRYRKEKAQSLAK
                     IISKTHDLTKLFFELLNGAICFYDRDPNDLGAKVDAKWAAFISNYGAITISLDTIPQS
                     FEKTMSWLIHGVSPSLVFIAQVDQTFHSSLLPLIIEQGEMNPRQIKLLQNMKADPEYY
                     QEEVEYYERKLQSISNEKTSY"
     gene            complement(4610..4717)
                     /locus_tag="ACHA44_002562"
     CDS             complement(4610..4717)
                     /locus_tag="ACHA44_002562"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905789.1"
                     /translation="MNQVNQHDLGEAIRVSREERGWTQRYLAEKVGISR"
     gene            4880..5692
                     /locus_tag="ACHA44_002563"
     CDS             4880..5692
                     /locus_tag="ACHA44_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905790.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6066..6257)
                     /locus_tag="ACHA44_002564"
     CDS             complement(6066..6257)
                     /locus_tag="ACHA44_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905791.1"
                     /translation="MKQKFFIAIIATKKFHRGKRFGQKWKFPPKVTAPQLKSIKELEF
                     ENALVICMTCTDEVFRKHF"
BASE COUNT         1916 a         1305 c         1006 g         2160 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagctaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataata
//
LOCUS       DAYYGS010000116         6274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000116 DAYYGS010000000
VERSION     DAYYGS010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.119"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2465
                     /locus_tag="ACHA44_002565"
     CDS             <1..2465
                     /locus_tag="ACHA44_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFM8905792.1"
                     /translation="EVITQKKHSVRDNDIEKIRFVFIDIDPKRKEGSATDSEKKKAEN
                     VMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTIQNFLQLLHRRFGV
                     EDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPDNIDRSNCFKILEQ
                     IVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIKEGDHKGVTLLIFD
                     ECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKYPLPKPVNPSVESL
                     ESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQKVITLVLAYYVDHC
                     WDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENAPIFFEHESLGWKK
                     ENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKYVLGTNLELAWSIG
                     IASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGNPTTVLRNMNATNK
                     AIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLDSNCQLMPQETFSV
                     LTLTSSEERLENYFNEKLTGLKVGYIEFSDIKFTKDANHAEKVKSLSSNTFGWGIKLF
                     LLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYSGALLIQDILGYPL
                     RLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLTNSDNKHTPSTIWG
                     KVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNMCAEKGRRTKRIHM
                     NRQVLPTYEILLPSSLKSYFRL"
     gene            2647..2886
                     /locus_tag="ACHA44_002566"
     CDS             2647..2886
                     /locus_tag="ACHA44_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905793.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            2902..4275
                     /locus_tag="ACHA44_002567"
     CDS             2902..4275
                     /locus_tag="ACHA44_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905794.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            4279..5913
                     /locus_tag="ACHA44_002568"
     CDS             4279..5913
                     /locus_tag="ACHA44_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFM8905795.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTSLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            6076..>6274
                     /locus_tag="ACHA44_002569"
     CDS             6076..>6274
                     /locus_tag="ACHA44_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905796.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYY"
BASE COUNT         2191 a         1025 c         1279 g         1779 t
ORIGIN      
        1 aagaggtgat tacacaaaaa aagcattctg tgagagacaa tgatattgaa aaaattcggt
       61 ttgtatttat tgatattgac cctaaaagga aagagggaag tgcaacagat agcgaaaaga
      121 aaaaggcaga aaatgttatg gaacaagtag aacgttattt gaaagaaaag agaatagaat
      181 catttgtaaa agttgatagt gggaatggct accatatttt tcttcccata aatgaacaac
      241 caaataatca cgagacgatt cttactattc agaattttct tcaattactt caccgaagat
      301 ttggagtaga agatgaagtt gatattgata cttctgttta taatccttca agattatgca
      361 aattagttgg tacaccagcg gtcaaaggaa agtccacaaa agagcgtccg catcgtttat
      421 ctagtttcct ttctattccc gataacatag atagaagtaa ttgttttaaa attcttgaac
      481 agatagttga agaaaattcc atgatgtcaa cctctagagt aaaaagccat ccatctttta
      541 gagaagtgtt accttttatt caagcagact ataaacaatg gttagctcac tatcctgaat
      601 taacttatac cataaaagag ggcgatcata aaggagttac cttacttata tttgacgaat
      661 gtccattaag aaaacacacg aataattcta atggttcttg tttgtctgtt acggctgata
      721 ataagattcg atttcattgt ctgcatgctt cggataaaaa taaagatatt caagactttg
      781 taaaaaaata tccgttgcca aaacctgtaa atccttctgt agaatcatta gaatccaatc
      841 aagaatatta ttttgaccag tttaaactga atcaagaagg agtatttttg gtgaaagaag
      901 gtcaagaaga aaaagttgct tgtccgatgt ttattaagca aatacgcaaa aatatcgaac
      961 aaaaagtcat tacacttgtt cttgcctatt atgtcgatca ttgttgggat gaaatagaaa
     1021 tagaaggtgg atgtttacaa actaataatt ttaaacaatt agcaaaacat ggcttaatca
     1081 ttaaagctag aagagaagct gaagtcgttg actttttaca gattcaacgg gagaatgctc
     1141 caatcttttt tgaacatgaa tctcttggct ggaaaaaaga aaatgaagca atgatttttc
     1201 agttacaaga ttcttttgga cgtgaaattc cttctgtatt aactaaaaac tcatgctacc
     1261 aattaacaac taatggtaca gaagcggaat ttgacgcatt ggttcataag tatgttttag
     1321 gaacgaactt agaacttgct tggtcgattg gtattgcttc tattgtgtta ggctatttaa
     1381 acgttacgaa ggctaatact tttttgagtt tactaattaa cctacagggc aagagcacaa
     1441 cgggtaaaac cactacgtta cactttattg cctctttatt tggaaatcca actacggttc
     1501 ttagaaatat gaatgctacc aataaagcca ttataaagct agcatcaaac aatcagggag
     1561 gtgttccact aatactagat gagttaggag cagcaactca aaataacttt agctcgttga
     1621 tctatcaatt agcttcagga gaagaaaggc taagattaga ttctaattgt cagcttatgc
     1681 cacaagagac tttttctgtt ttgaccctta cctcttctga agagcgtttg gaaaattatt
     1741 tcaatgagaa attaactggt ttgaaagttg gatatatcga attttctgat atcaagttta
     1801 caaaggatgc gaatcacgca gagaaagtta aaagtctttc ttctaatact tttggttggg
     1861 gtataaagtt atttcttttg aaactctttg aacaagggat tacaaaaatt gaagcaactt
     1921 ttgtcgaagc tcaaaaaaaa ctatgggctg ttttacctga acattctttg aagcaacgca
     1981 ttggtaataa tctagctttg atttatagtg gagccctcct aattcaagat atactgggct
     2041 atcctttacg attagagcat atacagacgg tgcttattaa ttcgtacgaa cagttaattg
     2101 tagatactag tttaggaagt gagcaaacgg tatttgaccg agtgaaaaca ttgttggtga
     2161 tgaatagcca taaattttta acaaacagtg acaacaaaca tacgcccagt acgatttggg
     2221 gaaaagtatc aattcaaaaa aatggggata ttaaggtaaa tatttttcct atcccgtttg
     2281 aggacttgtt aaggcgagaa tttcaagtaa aggatctaac ctatcttttt aggcaattgc
     2341 ttgattcggg taatatgtgt gcagaaaaag ggagaagaac gaaacgtatt cacatgaatc
     2401 gtcaagttct tccaacatac gagattctat taccttcttc actcaaatcg tattttcgat
     2461 tgtaatttag aataaaccaa agtatgaaag tgaggcacca tgttatggaa atatttctta
     2521 ttgatttagc aaaacaactg attacttatc tgtatgatag cgagcaaacc caaacggaag
     2581 taaatgaagc ttgatctatg cttcatttac ttcattcaat caaacatgaa agaaaggaaa
     2641 attctaatga ctaaaaataa aatgacacta aaagcagagg ttcttctgta tattcaagaa
     2701 catttttcaa atcaagcctt ttttacaaaa ccaatttact tggatttcga aataagaggg
     2761 gtatctgctg gatctatcgg gggtactctt caagcgttga agaacgaagg atatttagaa
     2821 aatcactttg tgcaacgttc gttcaataga agagtcgtga aaaaatggta tttagttcat
     2881 tcatagagag gggaatcaag catgcgtgtg gcttgttatg atcgaacgtc tgtttatgaa
     2941 gaagcgaaaa ttagtaatga cgctcaatat gatgcagtga aagagtatgt tttagagaaa
     3001 tatgaacaaa ttccccaacg attttcagac aaaggagaat caggggaaaa agcggagcgt
     3061 ccagcttttc gacagcttag agcttgggtt tgtgcgtcag aagaagaggg ggtgatctat
     3121 gtttggcgtt atgatcgatt ggcacgtcaa gccaaaatag ccttagaatt tattgagttg
     3181 tgccaacaac atcaggttca agtgtattcg atttctgaac ctcttcctgc ttctttctca
     3241 cacgcgtcaa cgaataaact atttgtgcaa ttattgttca cttttgcaga acaacaacga
     3301 aacgtcatta ttgagaatgt tcgtgccgga ctttcctata aaaagcaaca acggcaatat
     3361 ttgtcttctg cggtaccata tgggtatcgt ttagtgaatg ggaaaatcca acaagaagta
     3421 cacgaagcaa ggattgttcg ctatattttt caactttatt tgaccaaaaa atatggctac
     3481 aaaaagcttt gtcaacgatt gacgcaacaa aagcttttct tccgcgaacg tccttttcag
     3541 ccctatcata tttatagtat cctaaaaaat ccattgtact atggcgaaat caaaggagga
     3601 tctttgggga agtatctagg aacctttgaa ccgattctct cgaaagcaac ctttttacaa
     3661 gcccaagaga ttcgccaatc aaggtgtaca gctaaaaaag atacgtatct gtacttatta
     3721 cgtcaaaaaa ttcggtgtcc cttctgtggt cgtcatctga gttcgaagta tcaatggaat
     3781 acaaagaaga cgaagacgct tcattattat cattgtaccg accgaagctg ccaaggaatt
     3841 tttcttcggg cagaaagggt tgagtcagaa gtattggaaa agatacaggc tttttttcaa
     3901 tacgaaggcg tctatcaagc cttgattgcc caattaacgc aacaattagc cgaaaagcga
     3961 aaagatgaaa aacgcaaagt aatccaacaa aagaaacaag aagcacaatg gattaaggca
     4021 tttgaagagg gcaattgttc actggaagaa ctcaagcaac aactgcaagc tcttcaaaag
     4081 ccgatgtcta cttcttcgtt tctttcactc caacagtgtg aagcaaaagt gaaagaatgg
     4141 cttcaactaa aagacctctc tagtcgacaa ttattatgtg actatgtttg tcggattgag
     4201 gtaacaaagg ataaaacagt ttgtgggatt tacttccgac aactgacaca aaatatttta
     4261 gaaaggaacg aatgatctat ggaaatgata caaaccaaac gagtcgcatt gtatattcgc
     4321 gtgagtacac aagaacaagc tttagaagga catagtattc aagcacaaga acaaaatgga
     4381 cgacagtttg ccgaacggat gggctatgaa gtggtagaag tttatgccga tgaaggaatc
     4441 agtgggaaat ccacaaaaaa tcggttcgcc tatcaacgaa tgatgaacga tgctcggaac
     4501 catcgttttg atctactagt gatttggaaa ttgacccgtc tcggacgcaa tatgttagat
     4561 gtcttacaaa cagtcgagga attattgcga tatgacgtag gattacattc gatctcggaa
     4621 caatttgacg tgaccacttc gacaggtaaa ttgatgttac aactccttgg gagtttcggt
     4681 gaatttgaac gcaaccaaat tgcggagaat gttcaaatgt caatgcaaag cttagtgaaa
     4741 gataaaaagc gttatgcagg aggccgacgt ttaggctatg tcagtggtat tgacacgaat
     4801 ggtcaaaaac aattggtcat tgaaccagaa gaggcgaaaa ttgttcgtct gatctatgca
     4861 aaatatttag gcggagcagg gtatcgagcg attgccaatt ttttgaatcg tcaaggatat
     4921 caaacagtca aaaagaatgc tttttctacc acggcagtca aagatatctt acgcaataaa
     4981 atttatgcag ggtatttaga atatgcgcgc tacatcaatt gggaaaccaa acgacggaag
     5041 gggaagaatc cgagaccgat tttagtgaaa ggaacccatg aaccgattat ttcggaagaa
     5101 gattatcaaa aagtacaaga acgccttgct ttagaaggaa aacaacccaa atggaaccat
     5161 acaggtgaaa atgtattgac aggcttgtta cgttgtccag aatgtggcgc accgatggca
     5221 gcgagtaatg tcacaaatac cctaaaggat ggcacaaaga aacggattcg atattattct
     5281 tgttcagtct ttcggaataa aggagcaagt gtgtgtcatg ccaattcgat tcgtgccgaa
     5341 caagcggaaa catttgtcgc cgaccgatta aaggagatcg ttcagctacc agaagttctt
     5401 ccccgattgg tggcagcgtt aaatgaagaa atagtgcgac aaagccaacc attggaacaa
     5461 gaactcgtgg tgttactgga acgaaaggaa gaattgaaga ctaaaattga aaaatgggaa
     5521 gctgctttgg aagatagtcc tgaattgttt ccgatgttaa aagaccgctt agacgaattg
     5581 actgaaaaac gacgacagtt gcacattcga gaaaatgaaa tcttagggat ctttcaacaa
     5641 ggagagccga ttcaagtcaa agatgtccaa cggatattga ctagtttgga tcgttttctt
     5701 gcccagagtg agaagaagca aatcaaagcc ttgtatcgta cctttataga gaaaataacc
     5761 tttgatccta accataagga gatgttggaa attacgatga aattcacgca tgccgtggta
     5821 caacaattga atgagcaata tcaaatagcc gtatccaaaa caaaggatac ggctattttt
     5881 gtgttaaaaa caccctttac gttaacgatc tagtctaggt tatatcaaac aacgttagag
     5941 gggcgctaac gcttctctag cgatttgaaa tcgttagaga agtagttagg gaggtcctta
     6001 tcataaatag ggatatttat taatgaatat ttgaaatata attgtatata agaattaaaa
     6061 agagtgggga tagatatgaa aaagatgggt aagtgtattt tatgtgaaaa atacactgaa
     6121 cttactaaag aacatgtacc acctaaaaaa tcagggaata ctggaggtaa aaagaccaga
     6181 actggtaatt tagatgattt ccttaagagc gattttacaa aaggtgattt tccgaagggg
     6241 attaaaagga aacctcaagg aaatgtttat tata
//
LOCUS       DAYYGS010000117         6192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000117 DAYYGS010000000
VERSION     DAYYGS010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.120"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1326)
                     /locus_tag="ACHA44_002570"
     CDS             complement(<1..1326)
                     /locus_tag="ACHA44_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905797.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVSICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITS"
     gene            complement(1624..2559)
                     /gene="murB"
                     /locus_tag="ACHA44_002571"
     CDS             complement(1624..2559)
                     /gene="murB"
                     /locus_tag="ACHA44_002571"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFM8905798.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2632..3552)
                     /locus_tag="ACHA44_002572"
     CDS             complement(2632..3552)
                     /locus_tag="ACHA44_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFM8905799.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3665..4417
                     /locus_tag="ACHA44_002573"
     CDS             3665..4417
                     /locus_tag="ACHA44_002573"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFM8905800.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5281..5814
                     /locus_tag="ACHA44_002574"
     CDS             5281..5814
                     /locus_tag="ACHA44_002574"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFM8905801.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT         1863 a         1184 c         1045 g         2100 t
ORIGIN      
        1 agaagtgatt cgactctcaa tcacaatatt gtaattttta aatcctttca cttgagaaat
       61 aatgtaatcg gtataagcag atccttgcga aaaatcaata cagacatgaa tcggtgcttc
      121 aaaatatgat accggacaaa tactgaccaa taaaaagaga taatcataaa ataagcgaat
      181 catgaaacgt tcatcttttc ccaagaaata gcgtttattc tcttgtatga ccttttggat
      241 tgctgtactg aatattggat acgtctcgaa gaaaaactgt agctgctttt tagacgtaaa
      301 gctagtcatc tcaaagtcga atatccaatt ttttcgattg acatgcatca tacccatacg
      361 aagttctttt cgtatatata gtctttcttc ttcagagatt tcaatcgaaa atggtaattg
      421 atcaatcaat tccactgaca ttttttgact aatcatgtgg atatcatgaa aatgagcttc
      481 cgcaagtcca aatgcgccat ctaattcaaa ttcttcttcc gtgatcaaaa acaacatgat
      541 ataagaaatc tcatcaatga tctgctcttt agggatatga agaaaagaaa tatgtgataa
      601 atccttgatg atcttcattc cttctgagct cttaaagtat tcatctgaaa agaaacgttt
      661 tccgattggt tgattcccat aggccctcaa caaactaata gtggttagca aattgaattt
      721 gattcgtttt gttgcagaca gttttaaatt gaacagatat tcaaaatatt gcacgatact
      781 ctttgcttca ttcatgacgc tttctctaaa tggcatagaa aaaccattaa agaccccgaa
      841 atatacccca aataatacgt tccgtatgtt tatttcttcc ccaactaata tgtttttctt
      901 taactttatt tgatgctgtt caagaaaaga gactaattct cttcttttta cataggctct
      961 tgatatgctt aaaaaatgat tctctgcaaa tttttcgatc gttacttttg aatccacgat
     1021 ctccgttaac agtcgagcaa tttttgatcg ttgaaagtag attcttctta aatttttgat
     1081 atccgtcaaa ttcagacgtt cgacatggat ctctgcttct tctgaaattt ggatctctgg
     1141 ctttgttttg aattcagtaa attctttatt taattctatg atataatttc ttgttttaaa
     1201 tttagacaaa cctaatgttt catataactt aggcagcact acataattct ttttctcaaa
     1261 ttctaaatat ctagctagca gcagtttttc tctatcttcc tcttctataa ggtaatccaa
     1321 cttcatatcc tacacctcaa aaaatgcctc ataccaatta cttgatatgc agatgaaagt
     1381 tcatcttttc acctatactg attttatgat ggattatgct tgttttttca tcagttattc
     1441 tcatttttta tctttttttt gagaatattg caaaaaatgg aaaaatttat gaggaaatat
     1501 tctatatttt ttgttttatt gtgttaaaat gtacaagatt tgcgccattt gcgtaaacaa
     1561 ccacaaaaaa gagcaggacg gccaccgtcc tgctctttta cttattttaa gttccattac
     1621 ttattaattg gctaaacttc tgcttgtttc tgatattgct tgtttttctc caatgatccg
     1681 cacttctgtt tctaaagaca catcaaattt ttctttgatt acttcttgga tgtgcgcaat
     1741 caattcaata taatctgtcg ctgtcgcatg atcgatattg acgatgaatc cagcatgttt
     1801 ttcagagact tgtgcaccgc cccatttcag gccttgcaat cctgcttgtt ggatcagctg
     1861 acctgtatag tgtcctactg ggcgcttaaa gacacttccg catgaagggt attctaatgg
     1921 ctgttttgat tgacgcaatt cggtcaactc atccattctt gctttgattg cttcataatc
     1981 ccctgtttgc aaactgaatc gagcttccaa tacgatagca cgtaattctt ggactttact
     2041 gtgacgataa gaaaatacca tttcgtcttt tgtcaaagtt ttcaatgttc catcttcaag
     2101 caaaagatcc acttcagcaa aaacatcttt gatctctccg ccatacgcac ctgcattcat
     2161 ataaaccgct ccaccgacac ttcccgggat gccgcaagca aattcaaatc cagtcaatga
     2221 ttctgccaaa gctgcataag tcgtatcaat cagtgaggca cctgcttccg caatgaccat
     2281 cgttcctttg acatgaatct gtttcatttc agtcagcatg atgactacac cgcgaatccc
     2341 gccatcttga acgatcagat tgcttgcatt tcctaatacc atccaaggta tatcatggat
     2401 tcggcaataa tcgatgattt gctttacttc ttcttgtttt ttcgggaatg caaggacatc
     2461 agctggtcca cctgttttcg taaacgtata gttcatcaat ggttcatcaa acaatatagt
     2521 aatcgtggct aagctttcaa ctatttcttc tttattcact gaggattttc ctttcttttc
     2581 tcaaacgtcc tcattattct atcacgctta atacaatctt gccagtcttg ttcaaagttg
     2641 atgacggtag tgatctttaa gcacttcctc acataattca tgacttctta caacttcttt
     2701 ttgtttaagc aattctgttt ccgatttacc taaacggatc tgagaaaaga aggcctctac
     2761 cattggctca aacccgcgtt tgactagcgt gtttgtccag tcgccaaatt ctttcacttt
     2821 ttttccatca gcatcttgaa tgacaagctg ggtcagatcg tttacttcat aagtaccatt
     2881 tttacttgtt acttgatatt tttctatatt agcgccactt gctaaatcca tcgttaaaat
     2941 agccgtttga ttttgtgttt ctaattgtaa aatggcatat tccaaaaatt cctttgtttc
     3001 tcttatctgg tattttgttc tcaaaatggg ttcatctaat aaatacacag ctgtatcaat
     3061 aagatgtaaa aacaaatcat aaaggacgaa ctctgtcgtt tcttttgccg ctattcgatt
     3121 tttttgcagg tggatcaacc gcttatctgg gatacgtttc aattcttcta ccataggagc
     3181 aaagcgccgg ttgaaaccga tcatcaaaat tatttggttc ttatcagcta attcttgcaa
     3241 ttcacgaact tcatttagat ggacactcag tggtttgtca atataaacat ggataccatt
     3301 ttctagacat tttttggcta attcataatg tacttttgtt gctgcgtgga tcatacatgc
     3361 ttcgattttt tcttctaaaa gctcatcgat cgtttcatat agatgttgga acccgtactc
     3421 tttttgaatt ctttcttttg tttctttatt tcttgttgca aaataaaaat caagtgttcc
     3481 ttgttttttc gcataagttg gtaaatatgc tttttctgcg atattcccta caccgattac
     3541 accaatcttc atctactttc ctcctggatc atttagttac tttcatctta caccattttt
     3601 ttctaaaatg aggtacaata gtatcgcaaa ggtaaggaaa ttaattcgaa ggagtgtgct
     3661 tttattgaaa tttatctctt ggaacgtaaa cgggcttcgt gccatcgtaa ataaaaattt
     3721 tttagaagtt tttcatgagt tagacgccga ttttttctgt ctgcaggaaa cgaaactcca
     3781 agcaggacaa attgatttag atcttccagg ttattatcaa tattggaatt acgcggaacg
     3841 aaaaggctat tccggcacag cgatttttgc taaaaaacca gcattgaatg ccacttacgg
     3901 aatgggtatc gatatccatg atacagaagg ccgtctgatt actttggaat attccgattt
     3961 ctttcttgta acctgttaca cgccaaattc tcaaagcgaa ttaaaacgtt tagattaccg
     4021 attagaatgg gaagaagcct tttataatta tttggaaaat ctaaaaaaac aaaaaccagt
     4081 catcgtgtgt ggggatctaa atgtcgctca tcaaaagatt gatttgaaaa actggaaaac
     4141 gaatcaaaaa aatgctggat tcactccaga agaaagagcg gctctttctc gactgttgga
     4201 taacgggttt atcgatacat tccgttattt ttatccaacc caggaaggtg tctactcttg
     4261 gtggaactac cgattcaatt caaggaaaaa caatgctggt tggcggatcg attacttttt
     4321 gacgagcaaa gatttagagc caggactagc tgatgcaaag atccatacgg acattatggg
     4381 cagtgatcat tgtccagtag aattagattt gaaataaaga ggttgtgaaa aagtcgttta
     4441 gtttcgagta ttaagaaaaa ttttgtgaaa atggctttcc acattttgca taaatttggc
     4501 ataataccga gaaactggct tttgaacact gtttatcgag agattgtgaa aaagctgtcc
     4561 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
     4621 gcttattacc gcagatacaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
     4681 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
     4741 tgggcttatt accgcagatg caagcttttg aacaccgttt attaagaggt tgtgaaaaag
     4801 ctgcactgca tcaagtaata agaacagcca aacaaaaata gttcctcata tttttcgttt
     4861 ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattgaga agttgtgaaa
     4921 tcagcgtcct gacctgagta ttagagaaac aaatggcaaa atagttcctc atattttgct
     4981 attttttgag ctaataccga gggtcagctg attgaacacc gtttagtgga gaagttgtga
     5041 aaaagtcgcc tactttcgag tcgtaagaaa tttttttgaa aatggttcct cacaactttg
     5101 cataaatttg acttaatacc gagaagtcat gatccccatc taactccaaa tcacaaggaa
     5161 tgagttcaaa aactagcttt tctcaacaaa tattacataa aaaattctat gttttttgtt
     5221 aaatttattt gcttttttca aaagtatgtt acagtaattt cccaaggagg ttttacagaa
     5281 atgaattttg ttgccatgga ttttgaaact gccaatcatc agccttatag tgcttgttct
     5341 cttgcgctag tcatggtaaa aaatagccag atcgttgatg aattctatac attgatacag
     5401 cctgagactc cttttttctg gcgaaatgtc caaattcacg gaatccatca agaagacgta
     5461 cgcaatgcgc cgaaattccc agaagtctgg caaactatcc aaccatattt tcaaaaaaat
     5521 cgtttagtcg ttgcacataa tgctgctttt gatacaaagg tcttagcggg atgcttagat
     5581 tattatcatc ttgaacagcc taattatctt tctttatgta cagtgaaaag tagccgtcgc
     5641 ctgtttcctg aatttccaaa tcatcgattg aacactgtgt gcgaaaattt gaacatccaa
     5701 ttagatcatc accatgatgc attggaagat agtcgtgcat gtgcggaaat cctgctttat
     5761 caagaaaaaa aattcgggac agatccattg aaaaaacttg tgactataaa gtaaaagaat
     5821 acttatgaaa cacacaaaga ggtcgtgagc ctgccgtttc actccgagtc acaaggagca
     5881 atttcaaaaa atagcttctc atgtttatta aatattgtga ctggtgaccg aggagctggc
     5941 aggagaacac cgtttattgg agaggttgtg aaaaagctgt tctgcatcaa gtaataagaa
     6001 cagccaaaca aaaatagctt ctcatatttt tcatttggtt ggacttatta ccgcagatgc
     6061 aagcttttgg acaccgttta ccaagaggtt gtgaaatcag cgttttgacc tgagtattag
     6121 agaaacaaac ggcaaaatag ttcctcatat tttgccgttt tttgagctaa taccgaaggt
     6181 cagctgattg aa
//
LOCUS       DAYYGS010000118         6173 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000118 DAYYGS010000000
VERSION     DAYYGS010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.121"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            725..811
                     /locus_tag="ACHA44_002575"
     CDS             725..811
                     /locus_tag="ACHA44_002575"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8905802.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="ACHA44_002576"
     CDS             complement(932..1621)
                     /locus_tag="ACHA44_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905803.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1866..2780)
                     /locus_tag="ACHA44_002577"
     CDS             complement(1866..2780)
                     /locus_tag="ACHA44_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFM8905804.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="ACHA44_002578"
     CDS             complement(2762..3142)
                     /locus_tag="ACHA44_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFM8905805.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="ACHA44_002579"
     CDS             complement(3592..3879)
                     /locus_tag="ACHA44_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8905806.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="ACHA44_002580"
     CDS             complement(3899..4123)
                     /locus_tag="ACHA44_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HFM8905807.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="ACHA44_002581"
     CDS             complement(4578..4748)
                     /locus_tag="ACHA44_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905808.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="ACHA44_002582"
     CDS             complement(4763..5296)
                     /locus_tag="ACHA44_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8905809.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..>6173)
                     /locus_tag="ACHA44_002583"
     CDS             complement(5317..>6173)
                     /locus_tag="ACHA44_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFM8905810.1"
                     /translation="IVSRMREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEK
                     ILSLRFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYS
                     LQQFNELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPI
                     RQELTAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALAL
                     ADKQKEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLL
                     SDLDNLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DAYYGS010000119         5734 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000119 DAYYGS010000000
VERSION     DAYYGS010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.124"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1604
                     /locus_tag="ACHA44_002584"
     CDS             <1..1604
                     /locus_tag="ACHA44_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8905811.1"
                     /translation="NVTNLPTLPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTK
                     DTVKVPVEVTDNRSDADKYEPTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPG
                     GTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTV
                     DLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVT
                     DNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKG
                     TIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLP
                     KGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEG
                     DQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNT
                     SEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIF
                     TISGGLILLGTLGLLGYKNRKKENE"
     gene            1738..3081
                     /locus_tag="ACHA44_002585"
     CDS             1738..3081
                     /locus_tag="ACHA44_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFM8905812.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3193..4644
                     /locus_tag="ACHA44_002586"
     CDS             3193..4644
                     /locus_tag="ACHA44_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8905813.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4993..5625
                     /locus_tag="ACHA44_002587"
     CDS             4993..5625
                     /locus_tag="ACHA44_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HFM8905814.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
BASE COUNT         2208 a          817 c         1119 g         1590 t
ORIGIN      
        1 ataatgtaac caacctacca acattaccag aaggaacaac agtaacagat gtcactcctg
       61 gtggtacgat tgataccaac acaccaggta attacgaagg ggttatcgaa gtaacgtatc
      121 cagatggtac gaaagataca gtgaaagttc cagtagaagt gacagacaat cgttccgatg
      181 ccgataaata tgaaccaaca gtagaaggcg aaaaagtaga agttgggggc acagtagatc
      241 taacagataa tgttactaac ttgccagcgt taccagaagg aacaacagta acagatgtta
      301 ctcccggcgg tacaattgat accaacacac caggtaatta cgaaggtgtc attgaagtaa
      361 cgtatccaga cggtacgaaa gatacggtga aagttccagt agaagtgaca gacaatcgtt
      421 ctgacgccga taaatatgaa ccaacagtag aaggcgaaaa agtagaaatt ggaggcacag
      481 tagatttaac agataatgtt actaacttgc cagcgttacc agaaggaaca acagtaacag
      541 atgttactcc cggcggtaca attgatacca acacaccagg caattatgaa ggtgtcattg
      601 aagtaacgta tccagacggt acgaaagata cggtgaaagt tccagtagaa gtgacagaca
      661 atcgttctaa tgcagataaa tacaatcctg aaggacaaaa agtgactaca gacttaaata
      721 aagagccaga tgcatctgag ggaattaaga acaaagaaga tttaccaaaa ggaactactt
      781 atgcttggaa agagaaagta gatgttagca cagctggaaa taaaaaaggt actattgtag
      841 tgacatatcc agatggatca tctgatgaag ttgaagtaga cgttacagtg acagatactc
      901 gttccgatgc agataaatat aatcctgaag gacaaaaagt gactacagac ttaaataaag
      961 agccagatgc atctgaggga attaagaaca aagaagatct accaaaagga actacttata
     1021 cttggaaaga aaaagtagat gttagtacag ctggaaacaa aaaaggtact gtagtagtaa
     1081 catatccaga tggatctaaa gaagaagtag aagttactat ttctgtagta gataaaaaag
     1141 caccgaataa acctcaagtt gatcctatta cagagggtga ccaaattgtt actggtaaaa
     1201 ctgaaccaaa tgcagaggtg acagttacat taccagacgg aagtcaacac catgggacag
     1261 ctgataaaaa tggtaatttt acagttaaag ttcctaaatt agaggcaggg acaaaagtga
     1321 tagtaactgc aactgatgaa tctggtaata ctagtgaacc tactaatgta gttgtttctt
     1381 ccaacaaaaa agacagtgga aaaaacggta gtaaaggtag taagactgac aatcaaggta
     1441 gcaacagcaa tcaagacaaa aatagaggca aaagccagtc ctcaaaagtt tttcctaaga
     1501 cgggagagag tgacagtaat atctttacta ttagcggtgg tttaatactg ttaggaacgt
     1561 taggattatt agggtataaa aatcgaaaaa aagagaatga atagaaaatc aatttcttaa
     1621 gtcttctctg agaagaggaa gaatcttttt cttcctcttt ttgctttttt atgatgggaa
     1681 ataagtgtta gttaaagatg aatatacttt ttatagaaag gaaaagaatg taattaagtg
     1741 gaaaaaatag taattattgg ttcaaaccat tcagggctag ccgcagctcg atttctaaac
     1801 ttaagcgatt caaattatga agttattcta atcgataaaa aatcagaaat tagttacttg
     1861 gaatgtggga catcgttatt tataaaagga gaagtaagta atttagaaga tcttttttat
     1921 caaaatagaa tgacactaaa ttctgagata aaaaagttat ttctagagac agaagttgac
     1981 aaaattgata ttcaaaataa aaagattagt tgtgtaaaaa aagatggtac aagattctct
     2041 caagaatatg acaaagttat attagctaca ggttcaaagc aaattatgct agatataccg
     2101 gggaataatt tagctaatat tttttcgata aagaattttc caaatgcaaa agagattttt
     2161 aaagctttgg atgatagtac tattaaaaaa gtaggaatta ttggagcagg atatattgga
     2221 gttgaaattg ctgaagccat aagaaaaaga gggaaagaag tatatctttt cgatgttgca
     2281 gatagagtgt tgtcgactta ttacgaccgt agtttttcag ataaagttga agaaatacta
     2341 tcaaaaaatg gaatacatct ttgtctatca gaaagtccta aaaaatattt aggaagaaaa
     2401 aagattgaaa aaatagtcac tacaagtggt acccaatact ctatggatat ggtcgtacaa
     2461 gctataggat ttctatctaa tagtccaatt ggggaaaagg aactcttgag agatgttagt
     2521 ggagcatatc taactaatga ataccagcaa acaaatataa aagatatata tgctattggg
     2581 gattgtgcta caactttttt tacgtccatt aatagaagta gtgttgattt ttctatttct
     2641 aatgctctaa gaacagctta tattgcaact caacatatta acaatcaaga ttttacacct
     2701 tctggttccc aatttacaaa tggcttttct atatttaatt acaattttta cgcagcaggt
     2761 ttgaatttaa aaacagcacg attattaagt gagtctatag attatataga aatagaagat
     2821 ttcattagac ctgctttttt gagaaaaaat agtcgtgtaa aattaagaat tagctttgaa
     2881 aaaaatacaa agagaatagt aggtgtacaa ctttgtagtc aagaagattt atctggacta
     2941 ttatcaatgt tttctcttgc tatagaagaa caaattacat tagacaaatt gaaaatgtta
     3001 gattatttat ttcatcctag ttttagccaa ccctataatt ttattttaaa ggcagtaaca
     3061 ggattaaaca ttgaaacata atcgttacta aatgaaatca agaaatatac tgtattactc
     3121 aattcattga aaatatttat cataattttt aatagaaaac actattaaag agaaaaaaga
     3181 gaggtaaatc aaatgaaaaa atctttaaaa cttgcatcag cagttatatt aatgtcccaa
     3241 ctccttatag caacaccttt ccatgtgttg gcaagcgaaa attcaacata caaaattgca
     3301 aaagaaagaa ccgagacgac tgaaatgagt aattccgatg taacagcaga gagtagtgaa
     3361 cttttgacaa cttcatcaag tgaaagtagt acgacttcaa atgccgagtc ttcaacaact
     3421 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3481 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
     3541 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
     3601 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
     3661 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
     3721 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
     3781 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
     3841 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
     3901 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
     3961 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
     4021 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
     4081 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
     4141 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
     4201 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     4261 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     4321 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     4381 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     4441 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     4501 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     4561 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     4621 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     4681 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     4741 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     4801 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     4861 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     4921 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     4981 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     5041 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     5101 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     5161 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     5221 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     5281 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     5341 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     5401 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     5461 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     5521 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     5581 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     5641 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     5701 agaaaagatg ctgaaaataa attgtttcaa cctt
//
LOCUS       DAYYGS010000120         5728 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000120 DAYYGS010000000
VERSION     DAYYGS010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.125"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            196..921
                     /locus_tag="ACHA44_002588"
     CDS             196..921
                     /locus_tag="ACHA44_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905815.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGFSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="ACHA44_002589"
     CDS             911..1420
                     /locus_tag="ACHA44_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905816.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="ACHA44_002590"
     CDS             complement(1490..2938)
                     /locus_tag="ACHA44_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8905817.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="ACHA44_002591"
     CDS             complement(2938..3654)
                     /locus_tag="ACHA44_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFM8905818.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="ACHA44_002592"
     CDS             complement(3635..3985)
                     /locus_tag="ACHA44_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8905819.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="ACHA44_002593"
     CDS             4129..4902
                     /locus_tag="ACHA44_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFM8905820.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA44_002594"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="ACHA44_002594"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1663 a         1123 c          994 g         1948 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tttttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt ttttttaa
//
LOCUS       DAYYGS010000121         5651 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000121 DAYYGS010000000
VERSION     DAYYGS010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.126"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..955)
                     /locus_tag="ACHA44_002595"
     CDS             complement(<1..955)
                     /locus_tag="ACHA44_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HFM8905821.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDG"
     gene            complement(1026..1322)
                     /locus_tag="ACHA44_002596"
     CDS             complement(1026..1322)
                     /locus_tag="ACHA44_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905822.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            complement(1315..1608)
                     /locus_tag="ACHA44_002597"
     CDS             complement(1315..1608)
                     /locus_tag="ACHA44_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFM8905823.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            complement(1754..2305)
                     /locus_tag="ACHA44_002598"
     CDS             complement(1754..2305)
                     /locus_tag="ACHA44_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HFM8905824.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            complement(2479..2778)
                     /locus_tag="ACHA44_002599"
     CDS             complement(2479..2778)
                     /locus_tag="ACHA44_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HFM8905825.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            complement(2805..3113)
                     /locus_tag="ACHA44_002600"
     CDS             complement(2805..3113)
                     /locus_tag="ACHA44_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HFM8905826.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            complement(3230..4189)
                     /locus_tag="ACHA44_002601"
     CDS             complement(3230..4189)
                     /locus_tag="ACHA44_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8905827.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEVDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRRDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            4333..5109
                     /locus_tag="ACHA44_002602"
     CDS             4333..5109
                     /locus_tag="ACHA44_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905828.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            complement(5207..>5651)
                     /locus_tag="ACHA44_002603"
     CDS             complement(5207..>5651)
                     /locus_tag="ACHA44_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8905829.1"
                     /translation="KFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIKMAIASSSPMD
                     TINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEECLVIEDSYNGI
                     KAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
BASE COUNT         1694 a         1041 c          864 g         2052 t
ORIGIN      
        1 ttccatctgt ataaacacgg aattgttttc caccagccat tgttgtattt ccattatcat
       61 ccgttacaat attaattgaa tagtagcttc cattttcatc ttgcattaga tctttcatat
      121 attcatttgt gctaattaaa ttagtatttg atgcattttt atcaggagaa tcatacaacc
      181 ataaagcttt attatttagt acagtttctt tagcagcttc ccaacttgaa attgctgagc
      241 tacttgtaga ttgtgtcgtt gtagttgttg cttgttgagt agattcttct actactggag
      301 tttgaggagt ttgtccattc acaatatcaa tgaaaccttg ttttaactgt tggttctgag
      361 tttcattgaa gtacatgtag tttttatcat tgccttgatt ttgttgcgtt aaataaactt
      421 taggttgtcc attttgaaag ccaaagaaat atacatgctg ccctaaatat tctccagttt
      481 ctacatcaga ataaactgct actaaattga aatctgcatg accagttcca tcttctgacc
      541 attgtacggt aactggtgct tcattgattg ccattttcca atttccatca ataactgcac
      601 ttggtaaagg cgtaccatac agacttacat tgttctgagg attgtagctt ttatattctt
      661 gacctaatgt ctttccccat tgtgggacaa aaattgctag ttgagtagat ttctcactat
      721 cccacaaagt atttttaacc tcttcagaag aagattcaat gattgaactt tcacttgtac
      781 ttgcttccac agattgtttc atttctgttg tagtttgttc agttgattgc gttttacttt
      841 ttttagcctc aattgttgta tcagtttcta cagtctcttt agtcctcgta ttctcagatg
      901 tacaaccagt gaataaaatc cccattccaa ttagtaataa caaccatttt ttcattttat
      961 ttcccccttt caatttctcc ctcgcgtact tattttatca gtcaattaat ctcaatggtg
     1021 ttcatttagt taatcctttc ccaagaatgt cgatagccag catgacaacg atctcttcct
     1081 tgagggcttt cagatggatt aaaactagta gaaatttccc ttccacatcc tctacagcga
     1141 taagttctca ttatttttgg gctattagta ttatttttct tagttagagc tatgagtcca
     1201 actatgatta ccccaactgt cttaattact tttcctttat ctatattcgg atgcgcaagc
     1261 atatatttat ataaccaata tccaccgtaa attaaaccac ctaatacaat cagcttagcc
     1321 atgtactcgt ctcctttctt tttgtttaat tactttttga atccatcttg cagagaaacc
     1381 ataaacttct gtcaattgac ggatatctac agttttttcc tgttctatca tgttttcgat
     1441 tctttttcta actaactcac gatcgtacat tttcattgga aaagaaatct gttgccctcg
     1501 aaaagcaaga taaaattgat agagcaattc tgaatccagt tccatttcct caaaaaagtc
     1561 attcatactt ttataagagt tactcaaaca agtttcatca atctccatct acacaccccc
     1621 tgttattttt tacataatga aaatagcata ttactgggta cattgataat tcatataaca
     1681 tgtatcatca catgtatttt taaagtcaaa aagcatgtaa aactacttat aagtaacata
     1741 agaaaataaa atgttatttt tcttgaaata ataattgact atctgccaat tgccattcat
     1801 tacgaacatg aatacttagt gggtgggata acttaccttc tgcatttatc aatactttgg
     1861 tcatcttttt ttgtttcgca ctcaattgat cgtaaatggt cttcgcttgt tcataagcca
     1921 cttgactcgt cgctgtaatc gaaccacaag caaaaataat ggtgttgatt ttcttttctg
     1981 tgaaaacagt tgttaatatt tccaaagatt gttcatcatt tccacgagga aatacccgag
     2041 gacttttcgc ttttgtatac gaaaaaagat tcacagcaat aaaaccagta aatcctaatt
     2101 gtcggatatt cttttcgatt aataacatac tcaaatcatt cgtaaatggt tctgtatctg
     2161 ttggacgaat agtaatgact accgctaatt tatcttccgc ttcttttcct ttgatcgccc
     2221 attgtcgttt gaaaacatag cgatgggtat gatgaggatc tcgccaaact tctgtttgtg
     2281 tggtcactaa ttctttttca atcatctctt tttctcctat gctaattttt gttcaagcaa
     2341 ttcaatcacc attgccgtta taagtgtcaa tactagttga aaatatttca tattctttct
     2401 ccttttttct tcagaatttg atgaatcgct gtaaggatat cttctaaaca tttcgcaatc
     2461 attctcaaat aatcgatctt attcattcgt tcctccaatc tgactccaaa ccaatcgtcc
     2521 ttctaccagt aacccacgaa caaattctcg ctggctggta atatctgtaa tgaaataaac
     2581 tacatagtct tttccatctt ctggtgcgca ataaatataa agatgtgtca tcactaaacg
     2641 tggtagagta acatcttcag gaagcatacc catttcaatt tcctcttttt ccatcgtaac
     2701 ggattcatag ccccactctt caggagtagt gactagctgt gcacactttt ccagtacttc
     2761 gatagccgtt tctaacaagt cctttcgttc ctttctttta gatatcagta ttcttctggt
     2821 aataacatcg tttgatactc acccgaatca atgacccata ttttttgaga cactgatttc
     2881 aaacgaagct taggtgaaat cagatactct tttcttgctt ctggttcttc ttgacgatga
     2941 accaactttt gatttccttt ttccgtagaa aattcaaaga tttgaagata gtctaaatga
     3001 aattgctttt gaacgttttg atcgattaga ttccaacata aagcttgtaa ttccaccggc
     3061 aaactgtcca ttactccctt tgtgacataa cgttgtcctt tattttgaaa catttctttt
     3121 ccctcctttg ccataaaaat agcccaatct tctattaaag aagattgggc ttcatggata
     3181 taatatatat ttgatattca atttgattca ttttatacta tctgatttgt caaattaagc
     3241 tagacaatat gtctttatct atgcagctca aaaatacttc caatggtgtt cgataattta
     3301 atgattttct tggaatatgg tttcttttcg aggcaacgga ttgaataaag gtttcatccg
     3361 tgtccctaaa gtccattgtt ttaggtaagc catctcgtct aagcaagccg tttgagtgct
     3421 catttagccc tctttgcgaa ggtgttcctg gatctgcgaa aaagatggca atatcgttcg
     3481 agttacttat agatcgccag ttggaaaatt cttttccaca atcaaatgtc atggatttaa
     3541 acaagtgttt aggcatggat ttgaaccaat catttaacct gttttcgata tcttcggctc
     3601 gtctgcctgt cggtttcaaa gtaataataa ccttggataa acactcaacc aatgtaataa
     3661 cagcgctctt gtgtttaact cccacaatgg tgtcgacttc gagatgacca aattctttag
     3721 tcaggtgtgg atagagactg ttccgttcat gaatcgttct gcgaaaggcc tgttttccac
     3781 gcttttcttg atgattattc ggtttacgtt tccccttcat aggtaacgac tgtgtgtcaa
     3841 aaaggccttt tttaaacatc cgatagagtg tacgaacaga acaatcaatt ggaaactcgc
     3901 cacggccgat aatcacatcg ggtgaccatc cttgagctac cttttcttga acatatgttg
     3961 attgttcatc agataaaacg gttgaacgtc gtccacaacg agatttattt tgtttgtatt
     4021 gacggtaata atctagcact ttttttcctt gtttgagaaa cgaataaaca ttataaatgg
     4081 tttgtctaga gcgatttaaa ctttgtgcga caagagcgac aggtttctcg atattgaaat
     4141 aagactctat gattactagt tcgtctgttg taagatgggt gtaagtcatt tgtactcact
     4201 ccttattaat tctttggtcg gaactatttt gagtgtagca caaatgatct ttttagttgt
     4261 ctagcttaat tttacaatcg acgttttaat aaattgtgat ataatgaagt ataacttttt
     4321 agggagtgta ctatgaattt tcaagataat agaccattat atttacaagt agaagcttct
     4381 atacgtaacg atattcgtga aaaaaaatat cttccaggag agcaattacc tacagaagaa
     4441 gaattatgta aaatttatgg agtaagtaag attaccattc gaaaagcttt taaactttta
     4501 gcagaaagtg gaacagttga acgattaagg gggaaaggga cctttgtaac tcaaaaaaaa
     4561 gaaagtttaa aattagctgg agttcatgga tttagcgatt ctctttctac ccgaggacat
     4621 aaaattagat atactgtttt acattctaaa attataaagg ctgatgagtt tttagctaat
     4681 aaattacaaa tcaatgtaac tgatagagtt tacaatatta aacgtttaat gtgggaagac
     4741 ggtgccccga tgggaattga taattttttc gcatcagaag aaaaatttcc aactttattt
     4801 gaaaaaggta gccaatatac ttcattatat aaacttctag aagatgacta tgatgtcaaa
     4861 gctacgagct ctattatgga aataaacggt ttatctgcaa cacctgagct ttccgactta
     4921 ttgcaatgta tagtaggaga tccgctattt attttagaaa aggttggcaa agataaaaat
     4981 aatcaaccat ttcattattc tcaatccaca gttcgttgtg atagaattag ttatgttatt
     5041 gaagtcacag aaaatatcac tttagatacg gtgcataaaa atgattcaga tcctaaagta
     5101 atagattgat attaaaagaa atcataccca aagcaatcaa ttttgattag atttgggcat
     5161 gatttttatt tttactaata ttctctaaat acatcaaaaa acttatttat attccaaatt
     5221 tcacctgtac ttcttgtcgc agatatttaa tatcgtaaat aacatcatct gctagactta
     5281 catcttgaga aaaacgttta tctttaattg caagaacctt catatttgct cttttaccag
     5341 cttttatacc attataagaa tcttctatca ccagacattc ttcaactgga attctgagct
     5401 gttctgaagc tttaaggaat atagtaggat taggtttact ttctggcaaa tctcttccgc
     5461 taattaaaca atcaaaatat gactctattt tacaagtggt aacaacttca tttatagtat
     5521 ccattggact ggaagaagca attgccattt ttattttttt cttttttaac cattcaagct
     5581 gttcaattag tatattagga aaaatatcca agtaatcaat cttttgcgat ttccaatatt
     5641 tagtaaattt t
//
LOCUS       DAYYGS010000122         5624 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000122 DAYYGS010000000
VERSION     DAYYGS010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.127"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1087
                     /locus_tag="ACHA44_002604"
     CDS             <1..1087
                     /locus_tag="ACHA44_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905830.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1305..2447)
                     /locus_tag="ACHA44_002605"
     CDS             complement(1305..2447)
                     /locus_tag="ACHA44_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905831.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2452..2637)
                     /locus_tag="ACHA44_002606"
     CDS             complement(2452..2637)
                     /locus_tag="ACHA44_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HFM8905832.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2991..3236)
                     /locus_tag="ACHA44_002607"
     CDS             complement(2991..3236)
                     /locus_tag="ACHA44_002607"
                     /inference="COORDINATES: protein motif:HMM:NF024059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905833.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3230..3631)
                     /locus_tag="ACHA44_002608"
     CDS             complement(3230..3631)
                     /locus_tag="ACHA44_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8905834.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(3818..5623)
                     /gene="uidA"
                     /locus_tag="ACHA44_002609"
     CDS             complement(3818..5623)
                     /gene="uidA"
                     /locus_tag="ACHA44_002609"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010621381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFM8905835.1"
                     /translation="MYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESYP
                     ISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNGK
                     FITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKKE
                     NLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILIA
                     IYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGIR
                     SVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTSH
                     YPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKTV
                     IKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYLK
                     STPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTEY
                     GADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILRV
                     QGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
BASE COUNT         1817 a         1062 c          800 g         1945 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaaccac cggtggcaaa ccggtggcaa atccatgtaa
     1141 acatggttta ttttttacac tgttttcaaa caaacccttg ctatttcttc ggttcgtcct
     1201 cgtccatttt cgataccgac agctagctta taacccaagg tatatcaaca taaaaaaata
     1261 aaaataggct atgacagcag cgtgacagca aaaaagatgg aaagttaaat atcaaacctc
     1321 gtaatttcat ttgcgagtgc ttgatttgtt tctggatata agtgtccata tttgtccaaa
     1381 gtagttttaa tagaggcatg acctagacgc ttagatattg catagatatt tttattatgt
     1441 tcaattaaaa aggcaacatg cgagtgtctt aatccatgca tatgaattgg ttttactcct
     1501 gcaatttcag ctagtttttt tattctgctt aaaatagttc ttggagatgg cggtaatcca
     1561 tcaaaagaaa aaaccaattc tatatttttc ccaattgatt gttgagtttt tttccatgtt
     1621 tttaaatctt caacggtttg tctgtctaac gaaattatcc gaatacttga tgtatttttt
     1681 gtttcagcaa attcataatc ttgtcttgta cggatgtaaa ttgacttatt tacgctacat
     1741 ctgccattta ttaagtcaaa gtctttccat tgtaacgcta agagctcctc actccttaca
     1801 cctgtaacaa aaataaaacg catcaaacgt ttataaaatt cttcataaaa atccccgtga
     1861 taaacactat tatatacttt cttaaattca tcaactgtcc aaaattctac ttgaggacgc
     1921 tgagttttta ttttacctat agtatctaca ggattttctt caataatttc aagtacaatt
     1981 gcccttttaa aaactactct caacttattg aatatctgtt taatataatt tggtgataat
     2041 ggttgctcaa tgcccttttt tacaacatgg attttcgata aaaactgttg aaattcctgt
     2101 atatgaattg gtcgaatctt tacaacctgc atttctccaa aaaaatccaa tgcacgttgt
     2161 aaaatatgat ctgttttcat atatgttttt tctacagttc ccaacttata ccaaggaaaa
     2221 aaatactctc gataaaaatc atgaaacgtt attccttgca gataaattcc ttgtgaatca
     2281 gaaaaacttt gttttaactc ttcgagtccc cttctagctt ctccttgagt tttgaagcct
     2341 cttctggtca cttgaattct ttttccaaag cgatcttgtc ctaaagttgc agaaaaaaac
     2401 caagtgcctc tttttctgtc tttataaaca ttcgcaattt tcctcatttt actattcctc
     2461 ccaagaaata tggagaactt cttctaaaat tgaaatagga attcttttta tttttgaatt
     2521 acgataaaaa aagtatcctt tctcaactaa aagttcttta cattccttga agattttatt
     2581 agcatagtac tgagaaaaac ctagattaat caaatctttt cgagtagctg ttttcattat
     2641 agcaccttcc ttactttttt tcatttattt ctatcagctg cttttgtcaa aagcacatag
     2701 gaatttcacc tctccccaag tttacggcga gccttacgga agtatcatta tcccaacttt
     2761 aaatcatcgc tttttattga ctataaaaat tattttagaa tatttatcgc tccttcttta
     2821 cagcaagaaa tcatggcgca actaaaatat gctccttaaa gtgttaacgt actgatagaa
     2881 tctatttaat tttcaatgaa caataggttt tctacctaaa agaatctact aattccttaa
     2941 ctattagaag attcttttaa acagaaaaag tggaaagggg atttttttaa ttagttaatt
     3001 ttaaatttta agatcgcttc tattaattta atttctagct gacttatcat atattcatct
     3061 acataggtaa cgaattgacc tacctcgtta aatccatttg tcaaacataa cttagccata
     3121 taagaatgat aatgtttgag tatcatcttg atcgaaattg gatctccttc tagagcagat
     3181 aagataatgg atagtggaac taataggtac tgccgctttt tagatttatc taccataggt
     3241 cataccccat tttcaattgt tcaactgcct tctttcgttg ataccagata ccacttgtgc
     3301 tcatattgag aattttaccg atttcagcat cattaaaacc tgcaaaataa taaagtagga
     3361 taatctttct tcttatctcc gataactgat caattgctgc tgataacttt tgatctgaaa
     3421 ttaatatttc cattccaaat aagaaataga catcttgttc atcaaaatga gtttgggaat
     3481 acctgccttc atttgaatct gctaatggtt cctcattatc ctttatgtta gcgtttcgtt
     3541 ttcgaataga cttagcttcg tttcgtaata cttttttaca gtaggcatca aattggtgtt
     3601 gtatagatat gtcattgtaa tgttgttgca tgataatatg ctcctttttc cccctttcca
     3661 ctaatacaac aatcagaaag cccctttttt tcgaaaaaac tagaaaaaaa acataaaaat
     3721 atttatcatt ctttaaacag aaaatctttc tataaaaaac aaaaaaaaac gattaggcta
     3781 ggctaatcgt tttttgaaca ttcttaaaaa atccaaatta ttttttataa ctgaaattgg
     3841 gtattgatga ccatcgtttc tgtagatagt aagcaatcat tttaggtttt ctatctctag
     3901 taaagattcc tttcttattt ccttgcactc ttaaaatccc ttgacttgtt gcaaaatctg
     3961 caaaattcca cacttgttca ccaacaaaat tttcacatgt atctattact tcgtgatttg
     4021 cttcgtaata agccacttga tactcttctg taaacattgt tggtactgta tcatgaaatc
     4081 cagctactgt atctgctcca tattctgtaa acattattgg cgtatcagga catctttcca
     4141 tccatccatc tagctctttt ttcaacagat attttgcatg tgccagatcc cctcccgaaa
     4201 cataccaacc ataataacga tttaaacata aaacatcgac aatatctcct accttacaaa
     4261 catctggcgt tgattttaga taagtaacga ttgttacagg tcttttctgc ggatcaagtt
     4321 ctttagttaa ttcaactaaa ggttcaaaat attctttagc accttcggaa tccgaatctg
     4381 gttcatttgc aatagaccac attacaacac ttgggtggtt cttgtcgcgt tctatcaatt
     4441 ccttgatcac cgttttatga gaattttctg ttcgtatctc tttccacgta ttatgttttt
     4501 ccacattatc atctaataaa gtggccatga aatttaaatg taaccctact gcaggtactt
     4561 catcaataac tacaatacct tctcgatctg ctaatcgcat aatttcttct gagtaaggat
     4621 aatgtgatgt tctaaaagaa ttagcaccta tccatttcat taaattaaaa tctttaatat
     4681 ttacagcctc attcaaacca cgtccattaa catacgaatc ctcatgctta ccaaatcctt
     4741 taaaataaaa aggtttatta ttaatcaaaa atttgccatt ggtaacctga acactgcgaa
     4801 taccaaattc ttctgtatag gtatcgatca ctttattatc atcaattaaa ctaattttca
     4861 tactatataa ataagcgtct agaggttccc aaaaaattgg attcttaagc tgaatcattc
     4921 ctttctttcc ttcagcttca gctacaagtt gagcctcttc gtcatatatc gctattaaga
     4981 tttttccggt ccattctgta tcgatattga attctacact tgcatccaaa tcagttattc
     5041 ttgtattaat agtaatatca cttatatagt ctttcggagt tgtatataac ttaacaggtc
     5101 tatgaatacc tgcataattg aagaaatcaa aattaactaa gttttccttt tttatatccc
     5161 cattttcctg tactgtttcc ttcataatcc ccacaggtaa agtactatcg tcaataatat
     5221 tgtttacagc aactgtaatt cgattattcc cgtcttttac aaaccgatta atttcagact
     5281 caaatggtgt aaacccacct ttgtgttgag taataaactt tccattcaaa aaaacttttg
     5341 cttcatgtgt tgcagatcca aaacgaagaa ctaatctttc ttctaataat cttttaggaa
     5401 gagtcacttc tttttcatac cacacccatc caacatgatt tctaatgttc tctgtttcaa
     5461 ataaatcatt ataagaagag ggaactgaaa tcggataact ctctttttca tttattggtt
     5521 ctaaagcaag ttcttcctgt aagtcatcat ttgtatctaa tttaaatctc cagagaccgc
     5581 ttaagtcaat tacttgcctc gtctctgtca taatgggata caac
//
LOCUS       DAYYGS010000123         5576 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000123 DAYYGS010000000
VERSION     DAYYGS010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.128"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..614)
                     /gene="raiA"
                     /locus_tag="ACHA44_002610"
     CDS             complement(63..614)
                     /gene="raiA"
                     /locus_tag="ACHA44_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFM8905836.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            complement(742..1461)
                     /locus_tag="ACHA44_002611"
     CDS             complement(742..1461)
                     /locus_tag="ACHA44_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFM8905837.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            complement(1458..2771)
                     /locus_tag="ACHA44_002612"
     CDS             complement(1458..2771)
                     /locus_tag="ACHA44_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFM8905838.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            complement(2852..3172)
                     /locus_tag="ACHA44_002613"
     CDS             complement(2852..3172)
                     /locus_tag="ACHA44_002613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905839.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            complement(3280..3567)
                     /locus_tag="ACHA44_002614"
     CDS             complement(3280..3567)
                     /locus_tag="ACHA44_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905840.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            3694..4338
                     /locus_tag="ACHA44_002615"
     CDS             3694..4338
                     /locus_tag="ACHA44_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFM8905841.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            complement(4398..5105)
                     /locus_tag="ACHA44_002616"
     CDS             complement(4398..5105)
                     /locus_tag="ACHA44_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905842.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5325..5495)
                     /locus_tag="ACHA44_002617"
     CDS             complement(5325..5495)
                     /locus_tag="ACHA44_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905843.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
BASE COUNT         1602 a         1166 c          964 g         1844 t
ORIGIN      
        1 aaaaacttgc ggcagctgtc agtagctgct gcaagttttc gttgttattt aagtaaggct
       61 gatcagtctg tttcaatcag tccgtatttt ccatctttac ggcggtaaac gatacttgta
      121 ccatttgttt ctgcatcttc aaaaataaag aagttatgtc ccagcatgtt catttgtaag
      181 actgcttctt cactatccat tggtttcaat gaaaggcgtt tcgtacgtac gatatctaat
      241 tcagaatcat tttcgttttc gtcctcttca ttgaataaag cagtagcatc tgcagtattc
      301 atgcccgttt cacgggattt acgattaatt tttgttttga atttgcggat ttggcgttcc
      361 aatttgtcaa caaccaaatc gatacttgcg tataaatctg gagaagtttc ttcggcacgt
      421 aatacaagat aaggtaatgg gatcgttact tccacttttg ctgttttttc tgtataaact
      481 tttaagttca catgcgccgt tgcatcgggt gcgtcactga aatagcgttc tagtttgcct
      541 acttttttct caacgtagtc gcgaatcgct tcagttactt cgatattttc gccgcgtaca
      601 ttatatctaa acataaaaat tacccctttc gtctaccaca atcaagaagg agaaggacca
      661 ctctttccct cttcttaatt ctattatatc gaactctagg ttatttgcaa attaaatcgc
      721 tatcaaattt ccacatctaa ttcatcttgc taaagaaatg gttcggatga ttttcggctg
      781 tgcgacctgc aaacaatctg ctgcgtggaa aagagtctgt cctgttgtat agacatcatc
      841 aataatcaat atttttttat tttgtatctt agaaaaggtg actgcaagtt caaaaggttg
      901 aggcatctcc aatctttcct ttcgcgtgtt ttttgcttga gccagcagat gcttctctct
      961 tttgagtagc atctgaaaag gaattcttgc tgctttcaac atctcgctta cttgattgaa
     1021 tcctctttct gttcgccttt tttcagataa aggtattgga caatagatgt atgtcgggta
     1081 ttcttttttt atctgcgtcc atttatatgc aaaggtgcct gctagtcgga tatctcccat
     1141 aaacttgtat tgattgagcc aggccttcat attttcatcg tacacaaaaa aggcacggtg
     1201 ctgaaaatca taggtcggat atttcttctt ccattcttga cagtcactgc agtaaacggg
     1261 aatagacttt actttctttt tcgtactctc tagattgaaa tggattggtt tttgacagcc
     1321 agaacagcct cttgtttcca ataaatgaaa ttgtgcgcga caaactgaac aagtctgagg
     1381 taatctgtat ttcgaaacac caataatctc gctgaatgtt aaattttgga cgatatcttt
     1441 actgcagcaa ctgcacctca cagcatcttc ttcccttctt cattcatttc tttgatttcc
     1501 tgacaagctt ttcgaatagc tgcagttttt tcttcataga aaaagtatac ttgactgttc
     1561 gtataaggcc ctttacgatc ggctcttcct gcgatttgta ccaaagaaga tttagtaaaa
     1621 accggatgat ccgctcctaa aacgataacg gatattcttt caaatgtgac tccacgttca
     1681 aggatcatcg tggttaacag tatgtcatat tttgcttctc gcattttctg gactttttcc
     1741 gcccgctgat tgtctcttga agaaacatct gtgatctcat atctcggaag agcctcttga
     1801 agtgtggctt tcaaacgatg catcatcgtg atacttggac aaaataaaag gatatggttt
     1861 tctttaatta attgatgaat gatccgcacc aaagatttta tcttcttctt gttcttgcat
     1921 ttttgtcgcc aattattcat gaaaaccaag acgggttcag gaagtaaacg ccgatgaaag
     1981 cgcaaagcaa gtttgtggat gtcaaacgtc tgcttgattt cctttagtaa ctttttatca
     2041 ggtgtggcac ttaagtagat gaatcgtccg tctggtttaa tcgctgtttg cacagcatga
     2101 gctaatcctg catccccgtt ataaggaaac gcatcgatct catcgatcac tattaaatca
     2161 aaggaccgat aaaaacggta tagctgatga atcgtacaga taacaaaaga agtaaaggta
     2221 taagccgttg ctgtattccc atggagcaac gtgatttttt cttctggaaa aacataacga
     2281 tagcgtaaaa acaattcatt acaaacatcg actcgcggtg tacaaatcgc tactcttcct
     2341 ccagctttta aagtttccac taaaatagga taaagcatct ctgttttccc tgcacctgtt
     2401 acagcccaaa taagatgatg tcttttttct gaatggcaaa gtattctctt tgcgatttcc
     2461 gtttgttttt cagtaagctg tcctgtccaa aaaaagacga cttctcgagg ttttgcttca
     2521 ggctgatcaa acaaatataa atcttgttga gtgtcgcatc gccctaaata tagacaatgt
     2581 gtacaaaaat aaccgtaatc tgcaagctgt ccatcaatga cggacgtcct cattccacag
     2641 cgatgacaaa gaatccagtt gcccaaacgt tccactgctg cgatcttctc ataatttggt
     2701 aaatttacct cctcacagtc tattgcttta atatctctcg tcagaagcct tctaccccaa
     2761 aaatgattca tattattcgc cctaaaaaaa tatacgaaaa aacaagcgat ctattttttg
     2821 gatcgcttgt tcgatagtta tactgttcta tttattcacc tatgagcgtg attgcttgat
     2881 ctaacacttt ttcgccattc atcataccat aatcagccat attgatgacg tctaaaggaa
     2941 tgccttttgg ttccagacgt ttttcaaaat cagctttcat aaagcgaact tgaggtccta
     3001 ataacagtac atctacatct ttatttgcaa gattgttatc tgcgtcagaa gcggacacag
     3061 cgaaaatatc cgagtcgatt ccttttgctt cagcagcttt ttgcattttt gttactagta
     3121 aacttgtgct cataccagcc gaacatacaa gcatgattgt ttttttagcc atacttttct
     3181 ctccttttta aaacaaacta cgcaaacgtt ttcttcgcgt ctgttactct attatataaa
     3241 aagaaagaaa tttgtcaacg ttttctaaac gcggtttatt taatcaaata gccaactaga
     3301 tttcactaat ttattgaaca cgacatattc tttgtccaaa ttgatgacaa ctacttcata
     3361 gcctgcattt atccagccat aggcaccttt ttcagggtgt ttataatcat ttgcccacca
     3421 atctgtactt tcccttgctg ttttgggaag tccgttactg ggaaacagca gttcgtcaaa
     3481 ttgggtaaag gtcaacgtta cttgagagcc gccattgttc ttcaaataat gcgtaattgc
     3541 atcgtacttt ttcattcgtt cctgcatccc cagatccacc tacctttgtc tttattatcg
     3601 catactacag cgattgcgca accgttacag tgttgaaaca acctagaact ttatttatac
     3661 tgaataaaga aaatgaaaaa aagaggtgac aagatgatcg agcgctatcg tacaataaaa
     3721 gaaaatggac aaagtgagat tgagatcaaa aaatcacgct ttatctgttc tcttagacga
     3781 atagaatcag aggaccaagc aaaaagtttc attcaagaaa taaaaaaaga acattggaaa
     3841 gcgaaccata actgcagcgc cttcatcatt ggagaaaaaa atgaaatcca gcgaagcagc
     3901 gatgatggag aaccaagtgg aacggctggc gtaccgatgt tggaagtcct caaaaaaaat
     3961 caactgatca atgtagctgc agtagtcact cgatattttg gaggaacgaa actcggcaca
     4021 ggcggattaa tacgagcata cactcatgct gtctctcaag cattgtctga cattggaatc
     4081 gtagaaggta ccttgcaaca ggagatctct gtgatgatca cgtatccgca actaggaaaa
     4141 ttacagaatt atcttgaact ccaacatttt tttataaaag aaatccaata tactgatcaa
     4201 gttagcgtca tctgtgctgt agatgaaaaa agtgctgacc actttaaaga atccatcatt
     4261 gagctgctga acggacaagt ccaatttgaa gagggtcctg tttcttatca cgagcaactg
     4321 gtaactgatc atatataaaa acaggtgatt ctgattctct tttatttcta agtaaggaat
     4381 cagggatcac ctgtttttta tttttccaaa taaaactcaa aacgatttcc tacgtattgc
     4441 gtacgcacat actcaaacgg cgtaccgtct tcaaaataag aaatctggcg caaacgaaga
     4501 actgcatctc ctcgttttac ctctaaatag tcagcaattt tctcagaagc caacacagca
     4561 gtaatcgttt gattcgctgg accgatcttt ttcccgcctt tttcttctag cgtgcgatac
     4621 aacgattcag taatctctgc tttgctgtag ccatcgatca gcttttgtgg cacactggca
     4681 acttcaaaac aaatagggac atcatctgcg aaacgaatac gttccattcg caagatcgtg
     4741 tcatctttac tcaagtttag tttttccatt tcacttgagc ttggcgaagt caagaaataa
     4801 gagattgtcc gactagacgg tactcgattt tgcgattcca taatttctgt aaaacttgtt
     4861 gcaccagaca ttttttcttg tactttttct cgcgcaacat atgtacccga accgatcttt
     4921 ctttctaaga ttccttcatc tgccaagttt tgaattgctt gtctcaacgt catacggctg
     4981 acattgaatt tcactgctaa ttctctttct gaaggaagac ggtctccgac tttccatata
     5041 ccttgttcga tttcattttt taattggtca tgaatctgta tatatacagg taattgattc
     5101 gacataacgt acacccgtcc tttcgtttct tctattataa ctggtatata cctgattcac
     5161 aactgaaaaa gataaaggtt tctatccatc aacagaggat ttagacacac atcgttcttc
     5221 ctactcctat tgtcagaaac aattttttag atatgttcaa cctagaaaat aacaaaagac
     5281 cctaaaatcg atgaaaatgc cgacttatgg atcttttgtt attattagcg gtttttagcc
     5341 atccattttt ttgtatgatt cgttgggcgt tttgatttta cctggaccgc ttttccgcct
     5401 tttttcggaa tattcacttc aatattgtat ttttctgatt tgaccaaatc atataacttt
     5461 tcagctgctt cgtctacttt ttctttccat ttcatgtgga tccctccaat tcttgtagtt
     5521 aacttaactc tatcttaaat cagcagaatc ttcaaggaac aaggtagcaa agcaaa
//
LOCUS       DAYYGS010000124         5566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000124 DAYYGS010000000
VERSION     DAYYGS010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.129"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..300)
                     /locus_tag="ACHA44_002618"
     CDS             complement(<1..300)
                     /locus_tag="ACHA44_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HFM8905844.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKY"
     gene            393..742
                     /locus_tag="ACHA44_002619"
                     /pseudo
     CDS             393..742
                     /locus_tag="ACHA44_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(1014..1304)
                     /locus_tag="ACHA44_002620"
     CDS             complement(1014..1304)
                     /locus_tag="ACHA44_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905845.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="ACHA44_002621"
     CDS             1706..1942
                     /locus_tag="ACHA44_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905846.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="ACHA44_002622"
     CDS             1949..3901
                     /locus_tag="ACHA44_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8905847.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA44_002623"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="ACHA44_002623"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8905848.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="ACHA44_002624"
     CDS             complement(4797..5489)
                     /locus_tag="ACHA44_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8905849.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1810 a          971 c          902 g         1883 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttccagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaat
//
LOCUS       DAYYGS010000125         5274 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000125 DAYYGS010000000
VERSION     DAYYGS010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.130"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..546
                     /locus_tag="ACHA44_002625"
     CDS             <1..546
                     /locus_tag="ACHA44_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8905850.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYRNKLK
                     EYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYYNNER
                     IKQKLNWQSPVQFRKTTVTVV"
     gene            complement(716..1678)
                     /locus_tag="ACHA44_002626"
     CDS             complement(716..1678)
                     /locus_tag="ACHA44_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905851.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1680..3119)
                     /locus_tag="ACHA44_002627"
     CDS             complement(1680..3119)
                     /locus_tag="ACHA44_002627"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFM8905852.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            3304..5250
                     /locus_tag="ACHA44_002628"
     CDS             3304..5250
                     /locus_tag="ACHA44_002628"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HFM8905853.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKGTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1691 a          907 c          886 g         1790 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg gcgtatcaaa tgaaagctta tagaaacaaa
      301 ttgaaggagt acaaaatctt tcaaagcatg tctcggaaag ggaactgctt agataattca
      361 cttatggaga atttttttgg cttattaaaa caagagattt ttcacgggaa agtttataac
      421 tgtttcgagg aattaaaatc agccatcgat agctatatct attactacaa taatgaacgg
      481 ataaaacaga aattaaactg gcaaagtccc gtccaatttc gtaaaacaac cgtaacagtt
      541 gtgtaaaaat atcattttag agcatgaaaa aaggagtgga tttctccact ccaaaaaagt
      601 ctaacttttg ggggtcacta caagttgaca actccttttt gtttacaaat caattggata
      661 tctacaaatt gtattttcta acgaatttta tagacattaa aaaattaatt taacgttaaa
      721 tacttcctcc agaaagtatt gagattggta aaatataatc cttatttgtc ttttcgcctt
      781 taattttttt cagaagaata tcaacaataa gacttgcaat ttcatcaatt ggttgtctta
      841 ttgttgtcaa ttgtgggaaa aatttctcaa taaaagaggt accatcgtat ccaataattt
      901 ttaaatcttc tgggatatta agtttaagtt gatgagcaat tttcatagtg agaatggctg
      961 ttaaatcatc agaaacaaaa ataccatctg gtttgtgatt tataatagtt gactttattt
     1021 ccatttcacg acggatagta gacagattat taggaatttt gaaaattttt ccatttggat
     1081 tttgaaaaga aaaacctagt gcacgtaatt ctgtgggaga atccgtgtta tcatttcctg
     1141 taatcataat ggtattttga caaccatttt tttgtaaagt cttagctgca agttttcccc
     1201 cttcaaagtt atctgaagaa acaatggaaa cattaggagc taaatttcta tcaaaagcaa
     1261 caataggtgc tctcacacgc tcataatcag caattccaag attatggctt gatgagatta
     1321 tgccatcaac ttggttggct tcaagcattt caatgtattc tctctcttta gcaggatcat
     1381 tttgactatt gcaaataatc gttttatatc catgtttaaa taactctatt tcgagatact
     1441 caatgagttc agagtaaaag atattactaa tattaggaaa aataagacca ataagctgag
     1501 gtgactttcc ttgaagtcca cgtgctaaat tatttggttt ataaccaaga gttttcatag
     1561 cttcatgaac tttttgtttt gttttttctg ataaatatcc cttattatta atgacgcgtg
     1621 aaacggttgt tgggctaact ccagctaatt cagcgacatc agttaatttt gctaccattt
     1681 taatatttta attcaaagta ttttccagta ggttcgcctg atttgatttg aataccattt
     1741 tggtgatttt cagggaatac acgactagta aacacttttt ctcctttatt aataaaaatc
     1801 tcaatgattg agttatctac aaaaatgtta gcagttgttt ctttagtatc aattgagcat
     1861 tcacgaatcg taccaaagtc agtggcatat tgaatgcctg ctttgctgcg atctaaaata
     1921 atttttcctt ctttggtatc tacaatcaag ctaagtccat ttcctttact atcagagaaa
     1981 agaagtaact ccgatttctg atttggagga aaagttagtt caagctcata agtattattt
     2041 gtttctgctt ttgctgtaaa ttgttcagaa ctagcacgta atgatgtaat agcttctact
     2101 ggatattggt aaagtttacc atcttttatt gaaagttctt ttacaagact cattgctccc
     2161 tgataatcat acttatcagt tggataatcg atatcaggta gtccaatcca actgacaatc
     2221 agagcacgac catcaggagt attgaatcct tgagtcgcat acgcttcaaa tccataatca
     2281 agattttgaa tttctgaagc gcccacaagt tttacttttt ctatgtcaag cgattgacag
     2341 atcttaaaag tattcggata gatattccca taatttagct cagatttatc caacccttga
     2401 ggacaataaa tgagaacagg ttttttgtct acaaatacta gatttggaca ctcaatcatg
     2461 tattctgaac cagtgccgcc aaaatcaagg ttgccaactt cctcccatgt ttccacgtta
     2521 ttgtcatccg ctttgtaaag tttgataaag cctgattttt ctaaattttg tgctccaaca
     2581 atcgcataca attgtccttc ataattaaac atttgtggat cacgaaaatg atcagttaca
     2641 tcagcaggtt tatgaataag aacattctca cctttcgtaa tgttgtagtc tttatccatc
     2701 catgctccga tttgaagtgt atcacgttcc caattttcat ttcgaacgtt tcctgtatag
     2761 agtaagaata gattcccctc tatttcatat gcagaaccgg aataagcgcc gtggctatct
     2821 cgttttgtgt ctggaagaag ttctgctcct gttacatgaa aatgaactaa atcatctgat
     2881 tcattgtgta cccattcttt caacccatga gcagctccaa atggccaatt ttgataaaaa
     2941 agcgtatatt ttccgttaaa aaaagaaaaa ccattaggat cgtttaacaa tcctgtttta
     3001 ggttcgatat gatagctagc acgccaaggt gatcgtttta cattatcctt aattttatcc
     3061 atctcatctt tagtccaatc agaataagca cgatatcgta tttctcgagg taagttcata
     3121 ttttactcct tcaatttatc tttttacaac tagtatggta aacgttttcc gttgaaaaat
     3181 caatcatttc atttaaaaaa ataaactata tgacaaacgt ttgacatatt tttaagatat
     3241 gctaaaataa atttgcattt cagtgaaccg ttttcacgta aaaataaaaa aggagtgatt
     3301 cgaatgaatt acagtaaagt agcttccgac gtaatacaag cggtcggaaa agataacctt
     3361 attgcagctg cacactgcgc tacacgtctt cgtctcgtac taaaagatga cacgaaagtg
     3421 aatcaaaaag ctttagacga aaatccagat gtgaaaggca catttaaaat tgatggacaa
     3481 tatcaagtga ttatcggtgc aggtgatgtt aatttcgttt atgacgaact tatcaaaaaa
     3541 actggacttt cggaaatttc aacagatgat ttaaaacaaa ttgctagtaa agataaaaaa
     3601 ttcaacccca ttatggcatt aattaaatta ttatcagata tttttgttcc aattatacct
     3661 gcccttgttg caggtggttt attaatggca ttacaaaact ttttaacagc tgctggactt
     3721 tttggaccaa aatcaattga agaaatgtat cctgcgattg aaggtatttc ctcaatgatc
     3781 caactgatgt ccgctgcacc attcatgttc ttaccaattt tagtaggtat ttcagctgct
     3841 aaacgttttg gagcaaacca atttcttggt gctgcaattg gtatgatcat gacaacacca
     3901 gatcttggtg gctcaactga atattggaat atttttggtt atcatgtttc acaaacaaac
     3961 tatgcttatc aagttattcc agttttagca gctgtttgga tattatctat attagaaaaa
     4021 ttctttcata aaaaattgcc atcatctatt gactttacat tcacgccatt attatcagta
     4081 atgattactg gatttttgac tttcacagtt attggccctg taatgttatt gttgtctaat
     4141 ggaattacag acattattgt ttggctatat aacactactg gatttattgg aatgggtatt
     4201 tttggaggaa catattcact tatagtcatg actggtttac atcaatcatt tccagctatt
     4261 gaaactcaat tactttcagc gtggacaaat ggtatcggac atggagactt tatctttgta
     4321 gttgcatcta tggcaaacgt tgctcaaggt gctgcaacat ttgccatttg gttcttaact
     4381 aaaaattcta agacaaaaag tcttgcttcc tctgcgggtg tctcagcact tcttggaatt
     4441 acagagcctg ccttgttcgg agttaactta aaataccgct ttccattctt ttgtgcttta
     4501 attggttcag gaattgctac agcagtagca ggattactta aagtagttgc tgtttcactt
     4561 ggttcagcag gtttcttggg atttctttca attaatgcaa catctattcc attttacgta
     4621 ttgtgtgaat tgattagctt tgtagttgct tttgcaatta cttacttcta cggtaaaaca
     4681 aaagctgctg gtatctttaa agcagaagaa attgctgaac aaacaccagt taattcttca
     4741 aaagtagcaa caacagatcc aatatctact gatgttgaat caacacaaac aaaagtttca
     4801 tcagaaacaa ttgttagtcc catttctggt aaagtgatta gtctttcttc agtaaatgat
     4861 ccagtctttt catcagaagc tatgggaaaa gggattgcca ttaaaccaaa tggaaacacg
     4921 gtccattctc cagtagatgg tactattcaa ctcacgtttg aaactggaca tgcttatggt
     4981 attaaatcaa ataatggtgc tgaagttcta attcatattg gtattgatac tgtatcaatg
     5041 ggaggaaaag gatttacaca aaaagttact actaatcaaa aagttagaaa aggcgatatt
     5101 cttggagcat ttgaccgttc aatgattgag aaagaaggtc ttgatgatac aacaatgatt
     5161 attgtgacca atacttcgga ttattcagaa gtaaatatag tagctgaaaa tacagtcact
     5221 gagggtactg atttacttga aatcaaataa atatatttcc ctgagtaaag atgg
//
LOCUS       DAYYGS010000126         5224 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000126 DAYYGS010000000
VERSION     DAYYGS010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.131"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            50..721
                     /locus_tag="ACHA44_002629"
     CDS             50..721
                     /locus_tag="ACHA44_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905854.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            780..1220
                     /locus_tag="ACHA44_002630"
     CDS             780..1220
                     /locus_tag="ACHA44_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905855.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1232..1900
                     /locus_tag="ACHA44_002631"
     CDS             1232..1900
                     /locus_tag="ACHA44_002631"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFM8905856.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1964..2587
                     /locus_tag="ACHA44_002632"
     CDS             1964..2587
                     /locus_tag="ACHA44_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFM8905857.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2643..3029
                     /locus_tag="ACHA44_002633"
     CDS             2643..3029
                     /locus_tag="ACHA44_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HFM8905858.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3242..4633
                     /locus_tag="ACHA44_002634"
     CDS             3242..4633
                     /locus_tag="ACHA44_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HFM8905859.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1737 a          863 c         1096 g         1528 t
ORIGIN      
        1 tcttttcaaa atatgctatt ttaaaaatga tgaaaaaagg gggagaaaaa tgaatttttt
       61 agttgtttta aaagatactc tgattgagcg atttacattt ctatatgttc aattgcagga
      121 aaataaaaca ttgctgcatt tttcaccaga gttccatcta gaggggctaa cattaggaga
      181 aatctatcaa gcagaagggc tgtcggctgt tttgcacttt tttaaggtag aattggaatt
      241 accagtaaaa gattatatcg agctttctct tgaatcttgg gatcgagcag tggttgaact
      301 gtttccagaa ggtttaatga tcgatacaaa cgatgggaag atccatctga ctgcagcaga
      361 actgcaaaaa caaatgcgtt atcaaataga tgacgaaaat tctttttcta tttttagacg
      421 tcagcaaaag attctaaaaa gctttttaaa agtgatcagt aaaaaacgga atctgatcaa
      481 aacgacacag ctgttgaaaa aatatccagg tctgcttcac acgtctattc aatttcctca
      541 attgattacg atgatccatc gttttatacg tatgcaacag ctaccatcta agaagctgtt
      601 tcttccattg acggacacat ttcgagtagt caacagagaa gataaaaaga aagtgattgt
      661 gattgacttt accagaaatc ggaatgtcct tcaccaagtt gcaatggaaa aggccaatta
      721 aggctttttc tttttggatt tttttgtcat aataaatatt agatgatgga ggagataata
      781 tgatgcaaac atggttagaa aggctaatac gatttcagca gaactatcac aaacttatgc
      841 agggaagata tgcgaaattt gatcagttga atcgcggtct attgatcgct gcgcttgtct
      901 ttagtttgct aagtcgttgg ttgccctata gaatcggaca attgttgttc atcgtctttt
      961 tcggatggat gatatttcgt ttcctatcaa aaaaaatcta ccccagacta aatgagaatc
     1021 aacgttattt gaaatatctt gatagagtca aagaaaaata tcatcagata aaaaacaaaa
     1081 agcaagtcaa aatggatcga aaaagttata ctttttttga gtgcccgaat tgtcatcaaa
     1141 agcagcgtgc acctaaagga aaaggacgaa tacgtgtgac atgcaaaaca tgtggtataa
     1201 aattcgaaac gaatgtttaa gggagaaaag aatgaaatta gtattattac gtcatggcga
     1261 aagcgaagca aattttgaaa attactggac aggatggcta gatgtcgcct taacagaaaa
     1321 aggaaaagaa caagcagcag ttgccggtca aaaaatgctt gaagccggac ttcaattcga
     1381 tgccgtttat acctctgttt tgaaacgtgc gattcttacc ggacaagtcg cactagaaga
     1441 aatgggtcag ttatggcttc ctataataaa aacatggcgt ctaaatgaac ggcattatgg
     1501 ggatcttgtg ggtaaaaaca aagataagat gaaagaaata tacggaaaag atcaagtaaa
     1561 aacatggcgc agaggatata cggaagttcc gccgttaacc gcagaaaata gatttgaccg
     1621 cagatatgat cacttggatc cgcatttgat cccatacgga gaaagtctgg aaatgacagt
     1681 gaatcggatc attcccttat ggcaagatca tctagcacca aaactaaaag atcggcaaga
     1741 tttactagtt gtgggtcatg gtaatagcat ccgagcactg acaaaatatt tagaagatgt
     1801 tcctgaagat caaatggata cgatcgatat cccaaatgca caaccaatcc agtatacatt
     1861 agcagatgat ttgacaattc tggacaaaaa aatactttaa agaacacctt tatctaaaag
     1921 tgagagatag aaaaaagaag caaaggaatg gaacaaaagg aacatgaaaa attatatatg
     1981 ggactttgat ggtacattgt ttgatacgta tcccgcaatg gtagatggtg catggcaagc
     2041 actaaaagat tttggtatca gtatggacaa aaaagaaatc tacttcaaaa tgaaaaagta
     2101 ttctactagt tatttgatca atgaatcaaa cttgaatgcc agagagttta atgaattatt
     2161 tcaccgatat gaaaaagaaa gtacggaagt ttcccgtcca tttccagaaa cgaaacaagt
     2221 actggaaatg ctaaaagaca acggcggtcg tcatttcatc ctgacccacc gtttgacaga
     2281 atcaacatgg ggcctgttaa aagaacatcg attggcacat ctgattgagg aagtagtagg
     2341 gatcgatcag gactttcctc gtaaacctga tccagcatct ttaaattatt tgatcgatac
     2401 ttttcattta gaaagaacag acacgatgat gatcggcgat cgtcgtttag atatcgaagc
     2461 agggaaaaat gcaggagtcg ctacttgtct ttacgacatc gatcattttc tgggtgagat
     2521 cccggcagat tatgtggtag gaaatctgaa tgaaatcctt accttgcaga gcatcattaa
     2581 aaagtagtat cccctttcaa aggagatatg ttataatttt cataaaggag tgataaaatt
     2641 atatggaaat cagaagacaa aaagaagttg tagaagcatt tcattatgat atgagattgc
     2701 cgaaccaaga agtagaaaca gatttgaaag taggcttctc gcctttaaaa gaaacgatcg
     2761 aaaactatcc gaaagaacat agcatcgtag gagcacgctt agagttccgt ttagtgtttg
     2821 atacatatgt tttatctgga agtgtgagtc agatcaatca tatcctcaat cggaaaattg
     2881 aaggtcaaaa tgatgtcaca caagaagaag tggacgagct agtagcaccg ttattcgata
     2941 tcgtacgccg aatgtcttat gaggtgacag agatcgcctt agatcagcct ggggtaaaat
     3001 tgaattttca atcagaaaaa caagaataga cagtaaaaac atacctgcga tagattaaag
     3061 ctatcacagg tatgtttttt tattactaat tgtaagcaaa aagaatgtca tttcgataaa
     3121 aatcgtctaa aatataaaag tcactgaaaa aaattttttc aggaggatgg ttgaagaatc
     3181 attctcggga acctttactt ccttcgatgg acttgtcatt aataatatga ggaggagaaa
     3241 aatgacaaca ctcatcacag ttttagtggt tctagttttt gcggcattat tatatgtgtt
     3301 ctatcgtatg caaaagcgcc atacaaaatt ttcaacacgc gtctttacag cattaggagt
     3361 tggtatcgtc tttggcggtc tgcttcaatt gatctttggt gtagacgaca aagtcgttac
     3421 tcaatcaatg gaatggatcg ggattgtagg tcaaggatat gtatcattct tgcaaatgct
     3481 agttatgcct ttagtgttta tttcgatcat tggtgcattc acaaaaatga aagtttctga
     3541 aaagctaggg aaaattagtg cgactgtatt gacttcttta cttggaacaa cagctattgc
     3601 agcattgatc ggaattggtt caacaatgct gttcggactc caaggagcta aattcacaca
     3661 aggagctgca gaaacagcac gaatctctga actggcaaca cgtcaggaaa cagttgaaaa
     3721 cttatcgatt ccacagcaga ttttatcctt tatccctaaa aacgtttttg ctgatttagc
     3781 aggtagtcgt gcaacaagta cgattggagt cgttattttc gcggcatttg tcggaatcgc
     3841 ttatctaggc gtgaaaagaa aagcgccaaa agaagcagca ttcttttcag atttagtcga
     3901 cagtctgtat aaaatcatta tgcggatcgt gacgcttgtt ttgcgtttga caccttatgg
     3961 agtatttgct ttgatgacaa acgttttggc aacaagtgat tttgcagcta tcgtcaattt
     4021 aggtaaattt gtcgttgctt cttacgtagc attgcttgct gttttgattg tccactcact
     4081 gattttgatt ggtgcaaaag tcaatccaat caactatttc aaaaaagcct ttccggtatt
     4141 gagctttgcg tttacttcac gttcaagtgc tggagctttg ccattgaata tcgagactca
     4201 gacaaaagct ttaggagtag atgatgcaac agctaatttc tcagcaagtt ttggtttgtc
     4261 tattggacaa aacggctgtg ctggcgttta tccagctatg ttggcaacga tcgtcgcacc
     4321 agcagctgga gtgaatgtct ttagtttgga atttattttg atgcttgtgg caattgtaac
     4381 aatcagttct tttggtgtag ctggagtcgg tgggggcgca acatttgctt cactgatcgt
     4441 tttaggggca atgaacttgc cagtagcaat cgttgggtta gttatctctg ttgaaccatt
     4501 aatcgatatg gcacgtacag cagtaaacgt cagcggtagc atggtagctg gtatcgtgac
     4561 aagtgcacgc atcaaagatt tagatcgcga tgtcatcaat gatgattcta aagtgatcga
     4621 tgtgaatgca tagtcaaaaa gaggttgtga tagaagtggt cagctttaag aaataagaag
     4681 aagtttccta aaaatagttt ctcaaatttt tgtgaaattt cggcttattt ccgagaagct
     4741 acttctgaaa caccgtgaat aagaggttgt gaaaaagctg ccctgcatca agtaataaga
     4801 acagccaaac aaaaatagtt cctcatattt tacgtttggt tgagcttatt accgcagatg
     4861 caagcttttg aacaccgttt aagaaagagg tcgtgagcct gccgtttcac tctgagtcac
     4921 aaagagcaat ttcaaaaaat agcttctcat gtttgttgaa tattgtgtct tgtgaccgag
     4981 gagttgtcag gagaacaccg tttaccagag gttgtgactt gtttttagtc acgacctctt
     5041 tttttattta ttccatataa atacacatta tttattccga aaagtgaaag cgcaaataac
     5101 aaaaacaacc aacttattta ttattaaaga aaagcgtatt taagtaggac gattctgttg
     5161 agagctttcg tagattttcc cattttccta tggtaaactg acaaagttga gaatgttaga
     5221 acag
//
LOCUS       DAYYGS010000127         4954 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000127 DAYYGS010000000
VERSION     DAYYGS010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.132"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA44_002635"
     CDS             complement(73..1221)
                     /gene="nagA"
                     /locus_tag="ACHA44_002635"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFM8905860.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1297..2133)
                     /locus_tag="ACHA44_002636"
     CDS             complement(1297..2133)
                     /locus_tag="ACHA44_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905861.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2317..3534)
                     /locus_tag="ACHA44_002637"
     CDS             complement(2317..3534)
                     /locus_tag="ACHA44_002637"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFM8905862.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3683..3844)
                     /locus_tag="ACHA44_002638"
     CDS             complement(3683..3844)
                     /locus_tag="ACHA44_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905863.1"
                     /translation="MEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGIAMDWAA
                     NLWNDYHDN"
     gene            3917..4000
                     /locus_tag="ACHA44_002639"
     tRNA            3917..4000
                     /locus_tag="ACHA44_002639"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3951..3953,aa:Leu,seq:gag)
     gene            complement(4240..4954)
                     /locus_tag="ACHA44_002640"
                     /pseudo
     CDS             complement(4240..4954)
                     /locus_tag="ACHA44_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1518 a         1024 c          796 g         1616 t
ORIGIN      
        1 ttttactaga acacaaaaaa aaccttaaaa ccaaacttaa gaagtttagt tttaaggtta
       61 acttaaatta tattatgcgt ggaaacgttc cacgccatct agataagttg cttccaatgt
      121 catatcaggg ttcaatacga taaagtctgc atcgcgtcct tgagcgattt ttccacatgt
      181 atcatcgatt ttacagctga ttgctggaat cagactagcc atcatgactg cttgttctgg
      241 tgttgctaat ccccagtcta caacattttt aattgcttct ttcaatttca atatacttcc
      301 agctaagttg ccagattcta gacgtgctgt tccttctttt actactactg gaaattcgcc
      361 taaattgtaa tttccatcag gcatcccacc ggccatcata cagtctgtaa ttaatgcgac
      421 atgatcatgt cctgcttttt ccattaggat tcccgcagca tttggatgta catgatggcc
      481 atcacaaatc aattcagaaa agacatgttc taaagataat aacgcaccca ccatacctgg
      541 ttcgcgatga ttcaatccac gcataccatt gaaagcatgg acaaatacac ttgcgcctgc
      601 ttcaacagct ttttgcgctt cttctagtgt agcattactg tgtcctagag aaacaactac
      661 tccttcttca gtcacttgtt tgacaaactc ttctacacct tgacgttccg gtgctaaagc
      721 aatttttttg attaaaccac ctgaagcttc ttgccattca tggaaagtat ccaaatcagg
      781 atccccgaaa taacttgggt tttgtgcccc tttgtgttct tctgtgaaga aaggaccttc
      841 aaaatagatt cctttgattt tagctcctgg tgcttcctga tacattttgc cgattgtctc
      901 agctacatct tttaaacgtt ctttgcttga cgtcaatgtt gttggtaaaa atgaagtcac
      961 tccacaagat aacagacctt cagacatcac tttgatgcct tctgcatcat tatccattac
     1021 atcatgattc atatagccgt gaatatgtgt atctaccaaa ccaggagcaa tccatttgcc
     1081 gctttggtca atgatgttga cttctccctc aggtagttct ttggtgtatt caccaaaaag
     1141 tccatcggta atttcaagat agcctgcatg tttgacacct gacgctaaaa agaatttgtc
     1201 agcatagata aaagttctca ttctattctc tccctttgtt tttctacctc taaagtactc
     1261 ttatttagag aagaagtcaa gaatggtcta caccatttac aaattttttg aaatttgttt
     1321 cacagcttgt gctaagcctt catagaatgc tgctctttct ggttcttctt tacgcttgta
     1381 tattaagctt ttaagcacaa ataaatcatt gaggtaatag tagctattct tctcttgagc
     1441 ccaatagatt ccttgattca cctgttctaa cgcttcgtcg atttcatttt gatggaataa
     1501 gaaatttcca taattataaa aaatttgcga aaattcactt tttgatcgtt catcagctga
     1561 ttgatgcagc agttggatgc ttcgactcaa atagtagcgt gcttcagccg tttttcctgc
     1621 cacaccatat atttttccga tacaactaat cagttgtatt tctgtgtcag aaacatggat
     1681 tctttctttt tggctagagt agcttaatgc ttctttcaaa taataaaggg actgagagat
     1741 attttgacgc aacgtgaatt cgcaaacacc gagataataa taatagcgtt gaaagtcagt
     1801 tgcttgatac agattttcag aaatatcaga agattttagt acttgttcta acttccgata
     1861 atcacgtttt tggaaacaac tatccaacag ttcaaataat ttatcgtttg tccgagtagt
     1921 ttttcgagag agattcatca catgatcaat cgttacccct agccgttgac aaatctgcat
     1981 catcaccaat acattcggca gttcctcatt attttcgatc cgacttaata cactttgtga
     2041 acaaatatca tgcgaaagca ttttttgcgt cattttttgt tcttttcgaa tctctttaat
     2101 gatcgttcca aaggactcga ttaacgtttc cataagtatc ctccccaaaa tatgcgtatt
     2161 cgcattctat tcgtatttta ctttaggaag tcgcaaactt caagaaaaga cttgcaacta
     2221 aataatatac tttagttatt taagaaaact ccactagaaa gagagcctca gtgtgtttgt
     2281 ctgtggccct cctactgaaa aatttggttt ttaaaatcat ttttggcgat aggttgctag
     2341 gaattctctc attttcttag ctgtttgttt caaatcatcc attttaggaa gataaacgat
     2401 tcgaaaatga tctggctggt tccaattaaa tccaccacca tgtacaagta gaatatgatg
     2461 ttcatgtaaa aagtccaaaa cgaatttttc atcatccaaa atattgaatc gctttgtatc
     2521 aatttttggg aatatataaa aagcagcttt tggttttacg gctgaaagac caggaatatc
     2581 attgattgca ttatagatat attctctttg ttcatagatc cttccgcctg gcaggagcag
     2641 ctcatcgaca ctttgataac cgcctagcgc ggtttggata atttgctgag acaatacatt
     2701 ggaacacaaa cgcatggagg acaacatatt cagcccttct atatagcctt tgacgtgtga
     2761 cttatctcca cttaggacca tccagcctac tcgaaatcct gctacacgat gagatttgga
     2821 tagaccgttc agcgtaacaa cgaacaaatc aggcgctaat gtagcaatcg gaatatgttc
     2881 tagaccatcc atcactaagc gatcatagat ttcgtcggaa taaataatca ggttattttg
     2941 tcgagctact tcaacgattt cttctaataa ttcttttgga taaagtgccc cagtaggatt
     3001 attaggatta atgataacaa tagcttttgt ccgactcgtt atttttgatt tgatatcatc
     3061 aatatcagga taccattctg cctcttcatc gcaaatataa tgaacaggtg tgcctccagc
     3121 taatgcaata gaagccgtcc acaacggata gtccggcatt ggaaccagta cttcatctcc
     3181 attgtccagc aacccctgca tacacatcgt gatcaattcg ctcactccgt tgccggtgta
     3241 gatatcattg atcgtcacat tcggaaaatg tttcagttgg caatattgct caattgcttt
     3301 tctagcagaa aagatcccct tggaatctga gtagccttct gagtcacggg cattcatgat
     3361 caaatcacga atgacttcgt ttggtgcttc aaatccaaaa ggtgcgggat ttcccgtgtt
     3421 caattttaaa atactaatgc cctcttcttg catccgctcc gcttcttcta gaacgggacc
     3481 gcgaacatcg taactgacac cttctaattt gttggatttt tcaaattctc tcatcactta
     3541 gccaatccct catttcaaaa agttagaact ttccaaattt tttgttattt actatttcaa
     3601 tatcatactc catcctagaa aattttgcca cttatttagg aaaaacgtaa gtattaaaaa
     3661 aacacaaaca agcgttgaga acttaattgt catgatagtc attccataga ttcgctgccc
     3721 aatccattgc aattccacaa atgattccta gcataaaacc aagcaaaaaa cgtccgaatg
     3781 caagtccaat caatacacct aagatcattc ccagtcctga aaattctccg tcatatttct
     3841 ccatcttaat aagtcctccc atttttgagc ttgacaacta tgtaaaacct cgctatcatt
     3901 agtgtgttat tattacgcag atgtgttgga attggcagac aagcatgatt gagggtcatg
     3961 tgagcgttag ctcgtgtggg ttcgagtccc atcatctgca ctacaactcg tgaagttaat
     4021 tttgcttcac gagttttttt gtttgaacgt ttctttatgc cattatatac atatcactgt
     4081 atcctttaca aaaagaacgt acaatcatgt aaacagcctg aggtgagtat atgaaggtcg
     4141 gaaacaagct actcgtagaa acttgcaagt gttaaaacaa ttaatcgaaa aacgagtaaa
     4201 atccctcacc taatactaaa gcgagggatt ttttgtatat tatctgttta tcaccgttat
     4261 tataaaggta caaataccaa gcacggttaa tccaaattgc ataaattcat ttttttgtac
     4321 actcatgctt ttcattagct ctttaactgc cattagtact aagatggcga taacaacata
     4381 aagtatatag tacgtccgca ttccggaagc ccatactgtg ggagaaaatc ccataattaa
     4441 ccttgaacaa aatcccatta tcatcaaaat aactaaaaaa atagaagttt tcttattgtc
     4501 aaaacttaaa taaattccaa caattaaaca tagtaataat gccaagacta ctaaagtagc
     4561 tatccaagta cctggataaa aaagtgacag tttcgttcct aactctgtaa agagattatt
     4621 caggttacta ctactccaaa tcattgccct tttatttcca ttgacgtaag taaagctttg
     4681 acccattgta ttaccaaaat aaataatcag gtttaagaaa aacggtaatg cagtcaatat
     4741 acgcacataa tagttttgac atttcctata tgttagaagg aaaattaaga aaaatagcaa
     4801 taaaaataag atattcatgt ctaaaaaaac ggctttccaa atgatgaaat acctaaatct
     4861 aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat ttcatgtgca
     4921 gcacgtaatg cattccctgg agttgtcaaa gaga
//
LOCUS       DAYYGS010000128         4933 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000128 DAYYGS010000000
VERSION     DAYYGS010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.133"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..972
                     /locus_tag="ACHA44_002641"
     CDS             85..972
                     /locus_tag="ACHA44_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HFM8905864.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA44_002642"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="ACHA44_002642"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFM8905865.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="ACHA44_002643"
     CDS             2274..2720
                     /locus_tag="ACHA44_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFM8905866.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="ACHA44_002644"
     CDS             2993..4345
                     /locus_tag="ACHA44_002644"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HFM8905867.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="ACHA44_002645"
     CDS             4516..4803
                     /locus_tag="ACHA44_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8905868.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1696 a          813 c         1029 g         1395 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gaactgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt ttt
//
LOCUS       DAYYGS010000129         4915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000129 DAYYGS010000000
VERSION     DAYYGS010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.134"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1204
                     /locus_tag="ACHA44_002646"
     CDS             <1..1204
                     /locus_tag="ACHA44_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFM8905869.1"
                     /translation="AADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQAIINNV
                     DFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERYWFSFPK
                     NVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYHIGLYHG
                     EPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGHTKKEQA
                     STSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSKVEDGFS
                     LIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTFYTKTPL
                     AASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAKQLLNQD
                     FLFEEDQE"
     gene            1201..3921
                     /locus_tag="ACHA44_002647"
     CDS             1201..3921
                     /locus_tag="ACHA44_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8905870.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKLEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            3905..4849
                     /locus_tag="ACHA44_002648"
     CDS             3905..4849
                     /locus_tag="ACHA44_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HFM8905871.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         1819 a          815 c          989 g         1292 t
ORIGIN      
        1 tgcggcagat ttacatatgg atcggtcatt tgaaggactg acaactcttg ataaaactgt
       61 acaggaaaaa ctattgaaga cgaatctgac cgttttatca aatatcattg aacaggcaat
      121 aataaataac gttgattttg ttttacttgc aggagataat tttcatcaaa atcgtccttc
      181 attaaaaata cagaaacatt tttcagaaca gatgaaacgg ttagaaaaga accatatacc
      241 tgtatttata atattcggaa atcatgattt ctatcaaaaa gagcggtatt ggttctcctt
      301 tcctaaaaat gtccatttat ttacatccga aacggtagag acaaaaaaaa taacgataaa
      361 aagtggagaa actgtctctc tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga
      421 caaagtgatg gaatttcctt ctagagaact cacagattac catattgggt tgtaccacgg
      481 ggaacctggc gtctctcaaa aaggaaacta tgctcctttc caaccctcaa agatgcaaga
      541 aaagggatac gattattggg ctttagggca tatccatgtg ccaacggtct taaatgaaaa
      601 gcaaacaatt gtttatccag gtgcgcctca aggtcatact aaaaaggaac aagcttctac
      661 atctattcta ctagttgaat taagtaaagg aagttgccaa atacatccga ttaaagtagc
      721 agaggtctat tggaagacaa aggaaatatc attgaggaaa gcaagaacga caaaagaggt
      781 cattgttcac attcgtcagc agctttcaaa agtagaggat ggatttagtt taattgagat
      841 aaaattaaaa gattatgacc atctcaatac agatgttttg gaacggatac aatcaggaga
      901 gctattagac tatttgttag aggaattctc agataggata aatgatttct ttatttggcg
      961 tatttctctc atagagaata ctttttatac gaagacccca cttgctgctt ctgctaaatt
     1021 gatggaacag ttatttcaat actatcagac acctcaagat ttccaacaaa ttttaaatga
     1081 agtatatagt catcaagaag cggctagaat attgagcgaa ctgccggaat atcaagaaga
     1141 aacacttgaa aaagccaagc agctgcttaa tcaagatttt ttatttgagg aggatcagga
     1201 atgaagattt taaggatcga gattgctgct tttggaaaat ggcgtcaaaa aagttttgac
     1261 ttttattcag gcaatcagct gatctatggg ggaaacgaag caggaaaatc aacgatttat
     1321 caatttattc aagcaatatt atttggtttc ccttcaaaag gccgtaaaaa aaaggattat
     1381 acaccaaaag acggctcagc ctatggcggc aaaatatggc ttaaacatcc tgtatatggt
     1441 gaatttgctg tcgagcgcta caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg
     1501 gatcaagtgg gaagtgatga gttactagaa aaactgatcg cacctttgac gaaagagtta
     1561 ttccagcagg tatttacttt ccagcaagag cagttgatgg aactagatca tttgagagaa
     1621 aaagagctac atgatgcgct gatttcttta ggcatcacgg gcagttctca agtattccaa
     1681 aagcggcaag aatattatca gaaagcacaa caattattca agaaaaaagg ccaaaaatta
     1741 ctaatcaatc aaaaattaaa tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa
     1801 gaacaggaag cttattttgg caagttgatc caacaagaac aggaatacga gtcgcaacag
     1861 cagcagttat tggcacaatt aaaagaggcg aacaaacaat tttcattagc cagacagcaa
     1921 gaacttaatt tttctttata tgaagaatgg caatctctca aaaaaaacag cactacaggt
     1981 cttcctgatg agcaacttct tttagatctg gagtctttct ccaaacggta tcagcaaaca
     2041 aatgagcgtt tggaagaatt agaagcaaca ctggaaaagc atagcgggat ggatcagcag
     2101 tcagccagat attattttta tttagagcag gagcaggaac taaaagatct ccagcaactt
     2161 caatttacag cggaacaatt aaacgaagaa tggcaaagat tacaaattga gactagagaa
     2221 acaaattcag aattgaagat tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg
     2281 gaagaatttt tatatgaaga agaatggaag catcttctgg ctgaagaaag aaggattgct
     2341 gatctagaaa gccagatttc tgtgcgtctc caaatagcta aagaacagca agagatgatt
     2401 gaagcagaag tgaatgactt ggaagagacc tatccagcat tattacaaaa tcagtcagag
     2461 atagcaccag tctcttcatc taaaccgtat atgctgatgg ctggcggagc aataatcatt
     2521 gcaggtatat ttgtaccaat gcccttgaaa gtgatccttt tactaattgg tgcaggcatg
     2581 attggctatt tcttctacaa aaggcaacca aaaattaaaa ctaaaaatga tttctctgag
     2641 ataaaaggaa tgtggcaaga aaaactagga caacttgatc tagccttagc tcaattagct
     2701 gaagcagaag aaaaacaaaa gagaactgga gaagaaaaac agattcttca gcaagaagta
     2761 tatcgttttg cggatcatcg gcacttagga aagatggata cgctcaaact catgaaagaa
     2821 tatggacagg aagtcataga ttatcagaaa ttatatggcg tatttgagcg gaaacataaa
     2881 agagaacagg aaatacaaga aaaactacga caaatcgacc aaaaattaga gtttgttaaa
     2941 gaatggttac cgatacaaga taagacaatt tcagaaaaaa tggtattgct ttcccgtttt
     3001 atacaagaga tggaacagat gaaatttgcc agaagctatc agcaaaatac tttattgaag
     3061 caagaaatca atcaattgaa aaagaaacaa aaagtatgga tacaagaata cagcgaacaa
     3121 ttgactcaag caggcttatc ttatccttcg gaagtacctg tctttttaca aaaagccaaa
     3181 caagccgaac agcagatgaa aagatacaat gaattatcgg aaatgcttac tccactattt
     3241 cctgaagagc tgacaaaaga gaaattatct gtaagactgc gccaactgaa agaaaagcaa
     3301 tcccaactcc agcaagaaga aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa
     3361 gtagaagagt tgcaaagaga cggcacattg gacgaacttt atcaacaagc tagccggcaa
     3421 aaagcaaaat tacaggaatt actggaggaa tggggagcac ttgaaatcgc cgggacattc
     3481 ttaggtgatc tttcaacaga gatgagtgaa agacaattac cccagttgtt aagagcagca
     3541 ggtgattatt taggtatttt aacaaaagga aattatcaga agatattact gaaaaatgat
     3601 acactgactt taacagacgg atatacagaa tttcccatct atgagttatc tacaggtacg
     3661 aaagatcagc tgattatggc gatgagattt gcttacctct cattagaagc taaccgttcg
     3721 atatgcccga tcatcattga tgatggctgg cttcattatg atcatcagcg aaagtatcaa
     3781 ttagctgaac ttttagctaa atttggagaa aagtatcaaa taatttgttt ttcttctgat
     3841 aaagaaatgg taagctatta ccatgagatg aaccaaatcg taaaacaatt agaaggagca
     3901 aataatgaaa aaaattcgtg atttagtaat cgatgagcag tttgaacaat ttgtcctgat
     3961 caaaagtgca gatgtaagaa tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca
     4021 agatacttca ggtactatcg atggtaaata ctgggatgct tcggaggagg aaatcaataa
     4081 atttacagcc ggcacagtcg tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc
     4141 tcaagtaaag attcttcata tgcgcttaac aagggtagat gagccaggag agcctagttt
     4201 atacatggaa agagcccctt tgaagaaaga agaaatggaa gaagaaatca atcaattgtt
     4261 gtttgagatc accaatgcgc attggaatcg tattgttcgc tatctactta ataaatatca
     4321 aaaacaattt tttgagtttc cggcagcaaa aagaaaccat catgcatttg ccagtggatt
     4381 agcttatcat actttgacaa tgcttcactt agggaaatca atcgccgatg aatatcctga
     4441 gctgaatcga gcattgttgt atgctggtat cattttgcat gatttaggaa aggtgattga
     4501 actttccggt cctatgtcga cagaatatac gcttgcagga aatttgatcg gtcatctggt
     4561 attgattgac gaagaaataa ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga
     4621 agatgttctc gtattacgtc atatggtttt agcacaccat ggcttattag aatatggctc
     4681 acctgtacgt ccaagaataa tggaagcaga aatattacat caaatcgaca atatcgatgc
     4741 ttcgatgcag atgatgttga cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg
     4801 gattttcggt atggacaacc gaagtttcta tgtaccaaaa gatttatagc aaaaatttat
     4861 ttttttcatt caaaacaaaa aaagcgagac ataagtcttc gctttttttg ttttc
//
LOCUS       DAYYGS010000130         4794 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000130 DAYYGS010000000
VERSION     DAYYGS010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.135"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(79..1887)
                     /gene="pepF"
                     /locus_tag="ACHA44_002649"
     CDS             complement(79..1887)
                     /gene="pepF"
                     /locus_tag="ACHA44_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HFM8905872.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2063..3061)
                     /locus_tag="ACHA44_002650"
     CDS             complement(2063..3061)
                     /locus_tag="ACHA44_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFM8905873.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3287..3940)
                     /locus_tag="ACHA44_002651"
     CDS             complement(3287..3940)
                     /locus_tag="ACHA44_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HFM8905874.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4272..4670)
                     /gene="spxA"
                     /locus_tag="ACHA44_002652"
     CDS             complement(4272..4670)
                     /gene="spxA"
                     /locus_tag="ACHA44_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SpxA"
                     /protein_id="HFM8905875.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1404 a         1007 c          744 g         1639 t
ORIGIN      
        1 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
       61 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
      121 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
      181 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
      241 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
      301 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
      361 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
      421 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
      481 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
      541 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
      601 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
      661 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
      721 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
      781 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
      841 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
      901 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
      961 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
     1021 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
     1081 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
     1141 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
     1201 aaattgttcg taaacactgt acaacccctt aaatgctgct tcgcgcaccc ttcgatccgt
     1261 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
     1321 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
     1381 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
     1441 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
     1501 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
     1561 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
     1621 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
     1681 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
     1741 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
     1801 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
     1861 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
     1921 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
     1981 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
     2041 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
     2101 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
     2161 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
     2221 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
     2281 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
     2341 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
     2401 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
     2461 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
     2521 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
     2581 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
     2641 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
     2701 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
     2761 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
     2821 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
     2881 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
     2941 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
     3001 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
     3061 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
     3121 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
     3181 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
     3241 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
     3301 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
     3361 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
     3421 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
     3481 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
     3541 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
     3601 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
     3661 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
     3721 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
     3781 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
     3841 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
     3901 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
     3961 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
     4021 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
     4081 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
     4141 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
     4201 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt tccaagtttc
     4261 gtattatcct tctataatcc agccatcaat tgtgcttgac gtaattctaa ttgtcgtaca
     4321 tcgcgcggta ggaaccgacg aatttcatct tcattaaaac caacttgcag acgtttatca
     4381 tcaatcatga ttggacggcg aagcaatcca ggattgtttt gcacaagttc caataattct
     4441 tgtaacggca gatcatctaa atccatgttc agtttttgga atactttgga acgtgtcgag
     4501 atgatttctt ctgttccgtc ttctgtcatt tgtaaaatcg cttttaattc agaactgttc
     4561 aatggttctg aaaaaatatt acgttcaaca aatggaatct gatgttcttg caaccacgca
     4621 cgagcctttc tgcaggaagt acaacttggg gaagtatata atgttaacaa cgtgcaccac
     4681 tccttttcgt gataagaatc acttttttgt aacagaagat cataataatc tcccatctgt
     4741 ttctattata cataatcaac ttttaaatag ctaccttttt ttcataattt tttt
//
LOCUS       DAYYGS010000131         4756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000131 DAYYGS010000000
VERSION     DAYYGS010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.136"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(509..994)
                     /locus_tag="ACHA44_002653"
     CDS             complement(509..994)
                     /locus_tag="ACHA44_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HFM8905876.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(996..1667)
                     /locus_tag="ACHA44_002654"
     CDS             complement(996..1667)
                     /locus_tag="ACHA44_002654"
                     /inference="COORDINATES: protein motif:HMM:NF037286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905877.1"
                     /translation="MSRFSINVVEVDDYSEITDILKSIRKKYLRKTIFISGSAVDYTP
                     FTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGNSIVSGVLKQRRNLRKNNIQDVLS
                     LRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLESGEIVTADGMIEEFELSVQNENI
                     VVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQALEKLDTEGEVDKKVEQIVSLIN
                     SIQED"
     gene            complement(1930..3123)
                     /locus_tag="ACHA44_002655"
     CDS             complement(1930..3123)
                     /locus_tag="ACHA44_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905878.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3207..3407)
                     /locus_tag="ACHA44_002656"
     CDS             complement(3207..3407)
                     /locus_tag="ACHA44_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8905879.1"
                     /translation="MNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVIM
                     NGNRIQIKRKQFEKIIDTLDTI"
     gene            complement(3831..4064)
                     /locus_tag="ACHA44_002657"
     CDS             complement(3831..4064)
                     /locus_tag="ACHA44_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905880.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4071..4502)
                     /locus_tag="ACHA44_002658"
     CDS             complement(4071..4502)
                     /locus_tag="ACHA44_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFM8905881.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1228 a         1124 c          910 g         1494 t
ORIGIN      
        1 tcttttggga atcagaggaa agaatcaggc tttatagaga tatttcatct aacaaattac
       61 tttagactga cagtatatat cagcaggtac tgtcatcggg aacgtagcca atatcgtatc
      121 caaaacagca ggtagagaag ctatttgagg attcaagtta ttctatttca accatgaagt
      181 taagttcatt aaaaataaag atagttggta tttcaataat ttttgtatag agttaatcac
      241 tttattgatg ctaattcatc ttgattggta ttataatcgt attcaagttg cgtggcgtgc
      301 ttttgctaga aaatatataa ctgttcttta taaagaaata taaggattaa acatttttta
      361 tcatagagtt ttacttatct atagaattat gtctgttcct aattcagttt gatgaattaa
      421 ctgataaaaa aatatctact atttaaagat acagggataa gtaagtaaaa aaataacatg
      481 tcattagata gtagacagtt ccaactcttt atttaccttt tttgtatgct gcactgtcaa
      541 tccaagttcc taaattgttg tatccatcat cacttatata atcatattgg ccatcaataa
      601 tttcgtcaaa ccacaaatat tctccatctg gtttttgccc aattatttta attactgatt
      661 gactagtagt tgtcatctgg ttaaaatctt ttattttacc tatattaacg gccaaaatag
      721 catttcctcg cttataactc tcctggattt catatttaac atattctcga tcaagagttt
      781 ctttacctat cagcaccaca gtcactgatg ttccaattaa ttgattatct atccagtttt
      841 ttatagcaat atctccatta cgtttaactt tctcaaattc agccttatct acaaaaccag
      901 cttcctttgt tcctcgcaaa gtccagctgt ttctaactac cattgctctt gttacatctg
      961 cataatggaa actaaaaaaa acccttctca ccatattaat cctcctgaat actatttatt
     1021 aaactgacaa tttgttcaac ttttttatct acttctcctt cagtatctaa tttttctagc
     1081 gcttgaaatt tactttttaa ggaatcatca aaataatcac tgaaattctc ttgaatctta
     1141 tcaaacaaaa ccttacttgc acctttggta aatccaatcg gaactactat attttcattt
     1201 tgaacagata attcaaattc ttcaatcatt ccatctgctg taactatttc acctgattca
     1261 agtttgtttc caaaaacaaa aatactaatc ccagactctg caataatgtt ttctcgatat
     1321 cttctccact catgaggcat atcaagggga agtggtctta aagaaagtac gtcttgtatg
     1381 ttattttttc ttaaatttct tctctgcttt agaactcctg aaacaatact attaccaatt
     1441 ccaagtccgt aacctgatac tattctataa ccggattcag aaagcctata tgctaatttc
     1501 tcgacaaatt ttaatccttt cgattcagat gtaaatggag tataatcaac agcacttccc
     1561 gaaataaaaa tagtttttct caaatacttt tttctaatac ttttcaaaat atcagtaatc
     1621 tcagaataat catctacttc aacaacgttt atcgaaaatc tggacaaatc attgatttgt
     1681 agtcttcttt ttgtcagttc ataattatat ctctcttgat caaaaaaatc ctcagaatcg
     1741 acaaaatttt cctttaaaat tatatgggat tttagattat ctttttagaa gttcttgaat
     1801 gtcggtatag cccgtgtttc cgggcttttc cggcattttt gatattgtta gatacttaca
     1861 aatgttctta ttacctctca ccatttcccc ctcaaaagta gtaaatggag tagtaaaacc
     1921 ggccttgtgt caggctgcca gccgttccat ttctgccctt gcggaatcga atgtggcgtg
     1981 tgcataatag ttcagcgtca tggtgatatt ggaatgtccc ataatgtact gcaaggcttt
     2041 ggggttcatt cccgcattgg ctaaccgggt gcagaatgta tgacgcatag catggggcgt
     2101 cgtcgttttc ggcaacgctt cttcatggct cttgttgtac ttcttcacaa gcctgccaaa
     2161 catcccgcca taatctaccg ccgtcatggg atagccgttc tgtttgagaa acaggaaatt
     2221 ggcatacccg tcaataatga acggctttgc acctttgcgg ttttttatca ctcgctggaa
     2281 cgcctgatac acttcctcat tcatggggat ttttctgacg ccgctttggg tcttgggttc
     2341 gtcaatatag taaccgacgc cggtatttct caaaagctgg tggctcacga tgattacccg
     2401 gttttcaaaa tccaaatctt tgtccgtcag tccgcacagt tccgaaatgc gaagccctgt
     2461 ccccagcaga ataacgattg cgtcataatg cttttggtaa accttatcac cttgaataaa
     2521 ggacagaaaa ctttcttcct gtgctggtgt aagaggcacc tttggttccg tatcgtcgtc
     2581 cagaacgtca ctaagctgga agtcaaacgg gttttttcgg atgcaatcgt cctgtatggc
     2641 ggtgtaaaaa gccgctttca gggaacgctt gtcgttattg atggtcttat aagaaaggcc
     2701 cttttctttc atccgaagcg cccattcctt tgcgtcggac agcttcacac tgtcaatcgg
     2761 gcagctcccc agccgatctt gttccagaat acgcatcagc cgttcccggc ccagtttggt
     2821 gttgtgcttc acattgcccc ggtgcctgat ctgcttggcg tagagctggc agacggtcat
     2881 tttcttgccg atggtgtcta tcccgtcgtc aaggtctttc tggatttctt tctccttttc
     2941 ccggagtgaa atatcctccc gtttacctgc cggggtctta tccgtaggca ctaacttcca
     3001 tgagtaaaca aactgcggtt ttccaaaggt atcaacatat ttgtaagcat atcgtccgtc
     3061 ttttctctgg ctctctccag accgcaaaac gcgatttttg ctgtctcgtc ttttttctga
     3121 catctcgtca tgctcctttc catgacggaa agagccgcat atatgcttat aaattattat
     3181 aacatatctg cggccctttt tcactattag attgtgtcca aagtgtcaat tattttctca
     3241 aactgcttgc gtttgatctg gatgcggttg ccattcataa tgacccagcc agaggccgga
     3301 ttttcctcgg ccagcttacg caatttgttt tctccgatac ggaaatattt agacgcttcc
     3361 tcaatcgtca gcgtgtattt ttcccaaata ggcacatcat ggttcataat gcagccccct
     3421 ttcacaaatt caaaggtttg tgtgatttag gggcatagac ggacggctgc tggcacagct
     3481 ccacgggact ttcacccccg cgtggttctc acgcggccct acccattgcc tgcgacgctt
     3541 ttaacgctcg gactgtggct ataagggagt atcatagtcc tgcctgtacg tcgtcgcccg
     3601 caagccgcca cgctcacatt gcggcgtggt gttcctcgct ccgcttttcc cgtagggaaa
     3661 agtatcatgg cgcaccagcc gtccggctcg gcacagacag aatgtgtccg tatgccctat
     3721 gatgcgccgc cgttatcttg cgggcatatt tatcaaggtg cgatcaggcc accgggaaaa
     3781 caccgaaacg gggaaagggt aagccgtttc atgctgccgg ggccggttcg tcagttcgtg
     3841 acgatagcgt ggatgagctt gatttccaga cggcgcttca tggcctcgtc cagacacaca
     3901 tgcggatagc cgttttcgtc atagagcgtc cgggtacaca gcttgttcat gtaaccgctg
     3961 taatactgca aaacgcgctc tacggctccg gcatccccag cgcgggcggc ctcgatcacc
     4021 gaaaggggca aaagctcaat gcccttgtag ttcgggttct tcatccgcgt ctaacctccc
     4081 ttcggcatca acgcctttag ctgtttccgc agctccgata aggtatttgt cctgtgccgc
     4141 tgaacggcac tccgggacat tcccacaagg ccgccgatct caccatctgc catatccaga
     4201 acacagtgca ggattaaaat actctgctcc atgctgggca gcgcggcaaa ggcttcggca
     4261 acaagctcat tgttgatatg taggtcatag ccatgcgacg aaaacgtaaa gctgtcgctg
     4321 gggtagtgat ctactgtaca gagcttgtcc aaatctgcct gtgacaaaga gcttagtgac
     4381 acttcacggt cacggcggcg cttcatttca gccaagtagc ttttggcctc gttacgcagt
     4441 accgtcttgc aaaaagcatc gaacatgcac cgcgcttcat agtcgcgggg gatagcatcc
     4501 atcttctcac cccctttctg tgggggactt aggtggttct ctccctttcc gctaacaaga
     4561 ctttgacaag gccgccagct acccgctaaa tacctccttt ttgaaaattt ctcaaaaaat
     4621 ttttttgggg acagccgggc cgcaaaagac gacaaaaatc gcccggcagg tcgcaaacct
     4681 accgggcgag aaaatgcttt gctatgctgc tgatttacat ggtatagtac atactcataa
     4741 ccggaatagc cctgtg
//
LOCUS       DAYYGS010000132         4689 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000132 DAYYGS010000000
VERSION     DAYYGS010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.137"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(12..482)
                     /locus_tag="ACHA44_002659"
     CDS             complement(12..482)
                     /locus_tag="ACHA44_002659"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFM8905882.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(641..1099)
                     /locus_tag="ACHA44_002660"
     CDS             complement(641..1099)
                     /locus_tag="ACHA44_002660"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0043768 - S-ribosylhomocysteine lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFM8905883.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1270..2415)
                     /locus_tag="ACHA44_002661"
     CDS             complement(1270..2415)
                     /locus_tag="ACHA44_002661"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFM8905884.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2428..3345)
                     /locus_tag="ACHA44_002662"
     CDS             complement(2428..3345)
                     /locus_tag="ACHA44_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HFM8905885.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3773..4336
                     /gene="efp"
                     /locus_tag="ACHA44_002663"
     CDS             3773..4336
                     /gene="efp"
                     /locus_tag="ACHA44_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFM8905886.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4437..4607
                     /locus_tag="ACHA44_002664"
     CDS             4437..4607
                     /locus_tag="ACHA44_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905887.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
BASE COUNT         1461 a          850 c          881 g         1497 t
ORIGIN      
        1 tttttattaa gttaaagaac ggattcaatt gcagcggtca ttttcaatgg agaagtttgt
       61 ggtgcaaatc gttcgattac tttgccattt ctatcaatca aaaatttagt aaaattccat
      121 tttattgctt tagtgacagt accaggagct tcatttttaa gataagtaaa aagaggatct
      181 gtattttctc catttacgtc aatcaattga tgcataggga aggtaacgcc ataagtggtt
      241 ttacaagctt ctgctgcttc agtcgctgaa tctagctctt gtttaaattg attggaagga
      301 aagcctaaga taaccagacc ttgtgtctga taagcttggt aaagttcttc taattgttta
      361 aattgaggtg ctagaccgca ttttgttgca gtgttaacaa tgattatcgg tttgcctgca
      421 tattttttca atgaatagac agtgccgtct tctaaagtag tttcaaaatt ataaatagac
      481 ataaagtttt ctcctttcta ttttatataa taaaaagagc gctaaactga aaactagcta
      541 tttgttttcc cgtttagaac tcttttcaga caataaatat ttaataaaat acgaagataa
      601 gatagctata ttgcttctca tctttacgtt cttttagaaa ctatgcttct acttcagacc
      661 attcatcgcg ttttgctaaa aattcacgag ccaaagcttg tgcaccttct agtgtatggc
      721 tggcagccca gccacattgt gtttcattgc ttgcaggtac ttcagtagct tcaagaacat
      781 ctttcattgt tgcttcaaga acatctaaaa tgtcttcgta gctttcatga ttcaatacag
      841 tcaagtaaaa gcctgtttgg catcccattg gagaccagtc tacgatgtaa tctgcgtgat
      901 tgcggattag ttcggcagtc aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt
      961 ctttatttgg ttgtttaaag cggacgtcgt atttaatgat gacatcgcca tgcgcacccg
     1021 tttttcgatc agctacacgt acataaggtg ctttgacttt tgtatggtca agattaaaac
     1081 tttcgacatt cattctcatt tgtaagactc ctatctgttt gttcattcgt tttgatgctt
     1141 ttcactaaag aattatttag tgaaatttac aaaaacgtct ttctacttga ttctaacact
     1201 tatcgtttcc aaggtgtagc aatctgaaat attttttaga tttttctgct aagtcgttta
     1261 ttgggacttc tagttcttga ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg
     1321 accatactca attcctacag aaagacggat caagccgtcc tttatgccag cctcttctcg
     1381 tttttctttt ggaatagacg catgagtcat gacagaagga atttccacta aactttctac
     1441 gccacctaaa ctttctgcta aagtaaatag ttggagtgat tcaacaaatg gaatagcaga
     1501 ttcttcattt tttaaagtaa aagaaatcat tccgctaaag ccgctcatct gctgcttagc
     1561 aactccatgg agttcatgat cgggtaatcc tggatagtag accttttcta ctgctggatg
     1621 agaaaataga aaatcagcta ccacaaaagc atttttttga tgttcctcca tacgaacact
     1681 tagtgtcttg attccgcgct gaagtaacca gctatcttgg ggaccaagta cggctccgat
     1741 agcattttgg taaaatccga tctgatcggc tagatcttta tggttagaag tcactaaacc
     1801 agctactaca tcgctgtgtc cgcctagata tttggtaccg ctatggacaa cgatatcaga
     1861 accgagatct aaaggtcttt gaaaataagg agtagcaaat gtattgtcag caataactat
     1921 caatccatgt tgatgagcca gagtggcact tttttctaaa tcagtaatct ttagaagcgg
     1981 attgctaggt gtttcaagat aaagtgcttt tgtattgggt ttgatggatt gttctatttt
     2041 atcaagatta ctcgtatcta tgatcgtgta ctcgagacca tttttagtaa gaactttgtc
     2101 aaatagacgg aatgttcctc catagacatc atctcctaat aagatatgat cgccagcttg
     2161 aaaaagagaa aaaacggcat gaatcccaga aagaccagaa gaaaaagcga atcctcgaac
     2221 accgccttcc aaatcagcaa tcagttcttc caatgcaaaa cgagtaggat taccagatct
     2281 tgaatattca tattgtttag gctggccgac gccatcttgc cgataagtgg atgtttgata
     2341 aataggtaca ctgacagctc ctgttgtggg atcttcactg atgcctccgt ggatcaattt
     2401 tgtttgaatg tgcatgtttc tttcctccta taagtaaatg ttttttgata aatagcggtc
     2461 tgctgcgtct ggaaaaatcg taacgacacg atgaccaggt ggtaaacgac gaatttctct
     2521 taatgctgct gcaaaagccg caccactcga acttccaact aatagtcctt gttctcgtgc
     2581 taactgccta gtatagttaa acccttctac atcagagatt gtctcgatct gattgattga
     2641 aaggggagaa agaaacggcg gaatgaattc tacaccgata ccctcaattt cgtgaggggc
     2701 tggatcacca ccatttaaca cagagccctc tggttctaca ccgattattc gaatgtcggg
     2761 atatttttct ttcaaaaagg tagaagttcc agcaaaggtt ccaccactcc caattcctgc
     2821 tacaaaacta tggatatttt ctttgatttc atgaaagatt tctggcccta acgtatgata
     2881 gtaagcagca ggattatctt tattttcaaa ttgaagaggc agatagctgt ttgatatctc
     2941 ctctgcaagt tttttacttt tgctgatggc accgatgatt ccttgctcac taggagaatg
     3001 gatgatcttt gctcccaatg ctttcatcaa ttgttgtttt tctaagctaa acttctctgg
     3061 aacaacaaaa accgttctta atttgtatgt gagtgcggcc agagcgatcc caattcctgt
     3121 atttccggca gttggttcga tgatcgtcgt tttttcattg atttttcctt gggcaaatcc
     3181 ttctttgatc aaataactgc ccaatctatc tttgatactt cctccaggat ttaaatattc
     3241 tagttttgca taaatagcag aacctttagg catagaatag tgctgattgt caaagcgata
     3301 aagcggtgtg gaaccaatag cttctgtaat tgtcgagata atcatgtggg cctcctaaaa
     3361 aaagataata aaaaaaggag ctgactatta aatcagctcc ttgaaagaca tagagaaaaa
     3421 ttgagtagat aaaaagataa gttacctggt actcattcta tgaagggcaa ttaatagaca
     3481 tgacaaatag tatgctgctt ttatgcacca tacaacagca acgattcaat tgtttttggt
     3541 tgctgactgt catgtgtatc cctccaagta aaatttttat taacgatagc atgaaaaaaa
     3601 ataaaagtca aaggcttgaa agaaaaaaat atttcgatag catcgtttac ccgtgtttat
     3661 ttttctgaca aataggcatg agaccttaaa agatagagaa tactctagtt ttctggccgg
     3721 ttgtctgata caataaagtt gattatgata aagaaaagaa ggagcaaaaa taatgatctc
     3781 agcaagtgat ttaagagccg gtatgacttt cgtacaagac ggcaaactga ttaaagttct
     3841 ggatgcaagc caccataaac ctggtaaagg aaatacagtt atgcgtatga aactgaaaga
     3901 tgtccgttca ggtgcaacat acgacacaac gttcagacca gaagaaaaat ttgaaaaagc
     3961 acatatcgac acaaaacctg tacaatattt gtacacgatg gatgatacag ctttcttcat
     4021 ggatttggaa acatatgagc aatatgaaat tcctgtagaa acagtagaag aagaaatgaa
     4081 atatatccta gaaaacatgg aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt
     4141 tcaattacca acaactgtcg tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc
     4201 aacagtcact ggttcaggta agccagcgac aatggaaact ggcttagtag tcaatgttcc
     4261 tgacttcatc gaaacagatg aattgttgga aatcaataca gcagaaggtt cttatgtaaa
     4321 acgtgcgtca aaataattcg ctaacggatt gataagaatt tgaaaacaga gcttgacctc
     4381 ttgaaaggca gatcatcgga tctgcctttc aagaggtttt ttgtggaatc agcaaattga
     4441 aaaataaaaa agtatttgat tttatttata ttttgaaaga tgaatggcaa aagctagaga
     4501 aagaaaaaac tgcacttatt tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc
     4561 gaactattcc gaactatatt gaagcgaaaa tattttttca tcactaaata gtgttgaatt
     4621 tatgttttta tgtataaata gcttctaata gttcgggtat tttgttttta taaccatttt
     4681 tattcttat
//
LOCUS       DAYYGS010000133         4666 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000133 DAYYGS010000000
VERSION     DAYYGS010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.138"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            162..392
                     /locus_tag="ACHA44_002665"
     CDS             162..392
                     /locus_tag="ACHA44_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HFM8905888.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="ACHA44_002666"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="ACHA44_002666"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFM8905889.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="ACHA44_002667"
     CDS             1803..1979
                     /locus_tag="ACHA44_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFM8905890.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA44_002668"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="ACHA44_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFM8905891.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2594..3061
                     /locus_tag="ACHA44_002669"
     CDS             2594..3061
                     /locus_tag="ACHA44_002669"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8905892.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3146..4030
                     /locus_tag="ACHA44_002670"
     CDS             3146..4030
                     /locus_tag="ACHA44_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFM8905893.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4144..4380
                     /locus_tag="ACHA44_002671"
     CDS             4144..4380
                     /locus_tag="ACHA44_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HFM8905894.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4468..4548
                     /locus_tag="ACHA44_002672"
     tRNA            4468..4548
                     /locus_tag="ACHA44_002672"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4502..4504,aa:Tyr,seq:gta)
     gene            4555..4626
                     /locus_tag="ACHA44_002673"
     tRNA            4555..4626
                     /locus_tag="ACHA44_002673"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4587..4589,aa:Gln,seq:ttg)
BASE COUNT         1571 a          729 c         1021 g         1345 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcatattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaacaatt tggaaaatag cttcttatat tttgcaaaat tgtgagttat
     2461 tgccgaagtg ttggattttg aacaccgtca aaaaaaggtt gtgaaaaata cgggtagcac
     2521 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2581 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2641 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2701 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2761 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2821 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2881 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2941 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     3001 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     3061 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3121 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3181 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3241 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3301 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3361 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3421 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3481 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3541 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3601 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3661 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3721 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3781 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3841 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3901 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3961 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     4021 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     4081 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4141 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4201 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4261 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4321 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4381 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4441 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4501 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4561 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4621 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctt
//
LOCUS       DAYYGS010000134         4622 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000134 DAYYGS010000000
VERSION     DAYYGS010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.139"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(179..904)
                     /locus_tag="ACHA44_002674"
     CDS             complement(179..904)
                     /locus_tag="ACHA44_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905895.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1058..1384)
                     /locus_tag="ACHA44_002675"
     CDS             complement(1058..1384)
                     /locus_tag="ACHA44_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905896.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(1421..1786)
                     /locus_tag="ACHA44_002676"
     CDS             complement(1421..1786)
                     /locus_tag="ACHA44_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFM8905897.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(1823..2038)
                     /locus_tag="ACHA44_002677"
     CDS             complement(1823..2038)
                     /locus_tag="ACHA44_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905898.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            2167..2502
                     /locus_tag="ACHA44_002678"
     CDS             2167..2502
                     /locus_tag="ACHA44_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905899.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(2578..4575)
                     /locus_tag="ACHA44_002679"
     CDS             complement(2578..4575)
                     /locus_tag="ACHA44_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905900.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
BASE COUNT         1441 a          908 c          605 g         1668 t
ORIGIN      
        1 ttatatatca acgtttaatg aaactatctc cgtttcattt cagatacaaa taaatatagt
       61 cgggctccaa atccgaaaaa aaataccccc agtagcttat cctaagctac tggagatgta
      121 taagtacggt catataacaa cggggtaggg taaataaatg aaaaagatgg aagaatcatt
      181 attcaataac ttttttaaat acttcgttgt aatcagttac ctgtccgttc ttaaatacca
      241 cagaataatc ttttttccca ttttcaagta accgaagtgg aatatcttta ccaactttct
      301 ctgtcactgc attactgata cctgttagat tagctaataa atctgataaa gattgagcat
      361 ctgtagaaat ctctgtatca gtatttatcg tgattccttt atcttcattt acaagtttcc
      421 aagtaggtgg tatagctgtg tatgtttcta caaatgaatt aagtttttca ttaagtgact
      481 tctcttttat atttccatct aaaaactctg tatatatggc ttgagctata ctaaaacaaa
      541 gctggccttg ttctaataat tttacatcat tagttagtaa agctgtgttc acgttatcaa
      601 tcgcttcaag aagagtatct ttggtgtccc taacagcttt aggtgtgcct tctggaattt
      661 ccctacttga tacctcgtcc attttatctt ccataaaccc cttaaaatca tatctttctc
      721 ccaaatccat agaagttttt gagaaagctt tcatttctga gtctatttct tcaataatat
      781 ttaacatgtc tgatacatat tgtttaggct gattcgtttt taatctatat gtggtaaata
      841 tctcaattcc tccaattatc aataaaattg ccaaacaaat ccataataat tttttctttt
      901 tcatttttcc tcctatacac tgctgttata acagtgacta atgtaaagat tttcccagca
      961 atcatcagac tattatttac agggaatcaa aaaaactcct tagtaagctt acacctttag
     1021 gagttcctgt ttgaacggta tgcataatac atttaaatca atttaacatt aacacctcag
     1081 tatatggtat ctctacatat acacggtctt tttttggata gtactcgaca tttatattga
     1141 tagcacttcc attgtctaac gtagctttag cccggtacgt gttatcacca ttatcatgag
     1201 tgatcgtcac atcatcacat gtccgattta agttatactg ttcttgtaaa atctgagtta
     1261 ccaatggtac agatgacttc tctagtccag atgaacccgt cattcttgaa aaaattatac
     1321 ctataatagc caatacaacg atacctataa tgacaaactt cttattcata tttactttat
     1381 tcaacttttt atctcctatc tccaaaatga ttttctctca tcagtaactc attgacatcc
     1441 ccaatagtac accaaagatg gctccagcta cccctgctat catcaaaatc gtacccattt
     1501 gttcatcttt ctctcgatat aagtaaccca taataacagc aacagcacca aataccacag
     1561 gaataaatgc taatgatact accatagaaa tccaaccaat aatcaaatac agcttccctg
     1621 tctcttgtcc atgtacgatt tgttccttca tttcattaat tgtctgctca gtacttttct
     1681 ttactgtgtc tgttccggtt gtctcatctg tttcttgtaa tgctccgcaa aactggcaat
     1741 acttactttc atttgttatt tctttaccac attttctaca atacatgtct tctcctccta
     1801 aaatcattca attgcttatt agctaactgt ttactttatt aaatacatca gaaaacagct
     1861 catttattgg tacacttaat atctcagaaa gcattttcat ttgatagggt ttaggaatcg
     1921 ttctccctgt ttgccacgaa cttacacatt gctgtgtaac attcattttt ttggctaact
     1981 cagtttggga aagtcctttt tcttttctgt atcggctgag attacttact tttttcatga
     2041 ttctcccctc ctgttttatt atatacaaca atatgatgta tttcaactca taaaatatat
     2101 aaatacttca aatgtcatta caaatacaac aacttgttgt actctactga gaaggggggt
     2161 gtattaatga aacagaagca atatgcaaag gtcattagag aaaagcgaaa agcactcggg
     2221 tggacacaaa aagaattagc taaaaaaatc ttctctacac aacaggcagt agcaagatgg
     2281 gaaaattctg taacagaacc taacctcgat agcttaacag ctctttctag agccctagga
     2341 acccctgtaa gccattttct agacaatgtt gacatagatt atgaggaaga gtttttggct
     2401 ctctatcgct ctcttagtac tgaagatgca attcgaacaa ttgactatat gaagctatta
     2461 aaaaggcaag aaaatgaacg caatcagcta ttgaaagatt gaaaaataaa taacccaccg
     2521 aactttaacg gtgggtgtat tgagatgaaa taagattaag agctatggat aacctgtcta
     2581 gtttaatata attgggtctc ttctattcct tactctccgt ccatcatctc ttgttacact
     2641 tgtaaattga taaataggaa ctaattcaac tcctgataat atcaattctt ttattacttc
     2701 atctatccct gcccctttcg ctagatatac taaaaaacta ctgcttgctt tcacattgta
     2761 tagtttctta atatccttag aagctcgttc aagagtgaat ccttgtttaa caaagtagcc
     2821 tctgttttct attaaagcat ttttcacgct gttatccctg acatttcgta ggcgttcaca
     2881 aacagtgtta ataagttctt tttctgtcat tagatatgtc ccttgccacg ataagccctg
     2941 taaaacttca tgtgcttcat ctaaaatcaa ttgtttttcc ttgctaagtt ctgatcggga
     3001 attttgagga aatactcttt tagcctcttt ttcgttaaaa catatcttaa cccattcata
     3061 agaaaaatat tgatatgtaa aatgaaattt ttccagaagt ggaaggatat cttcattcaa
     3121 tttttgaatc acagaagttg cattaaaaac gtctaatcga tagacattgc ttctcttatg
     3181 tttaaagttt cctaacgctt tctcttcctt taacctattt aggtattcga gcatcttcaa
     3241 aggaacatct tcatcatcta atttttttat caatcctata aaagtcataa gtgatagtaa
     3301 cttattacat ttatctatgt cataagtttt accatagtaa gaactcattt ttttagctaa
     3361 ttgttccccc gacaaaatac aagtcacact ttccttacta tcatcaaaaa aattaacatc
     3421 agaaagcaag acatcataga cacgtcgcat ttcctctaga taattcctta ttgtcctgaa
     3481 aagtttgtct ttcattgatt caagagtgtc aaaaagtact tttgattcct ctttcaattt
     3541 tcgaaaatgt gtaggataca agtctaggtt ctctaatagc caattaaatg tttctacccg
     3601 actgtctttc tttaaaatta gcatcacaac tccaataaaa ttttcactga tatcacaatt
     3661 actggaatga caataaaaaa tggaatgccc agttttactg gtaaaaatac tagcagaggg
     3721 attatgatcg ttgtgaaaca gacatcttaa gtttttactt gacttaagta atttattcat
     3781 atcaatggtt aacaatcttt cataaatctc attaaaagac atgtcggagg aatcaaacag
     3841 gcagtcttta aaccactgtt tcatttcttc tttgtcgtca ttttttatca acttaacaaa
     3901 atcggtttca ttgactagtt catactctct ccctgaatca ctttgacaat ttttagaaca
     3961 tgtcatggta aaatcaatcg gaagctcagt aatatctaac atgttgtctt cattaattat
     4021 tatcactgca tctgacttat atcctccaaa aaacattcta ttcgaagcat ttacattgtc
     4081 atcacacccg gggatgttct ttttcaagta ttcgtaaaca gcaaccattt taccatagtc
     4141 agtaattgct tcttttaata gaaagacaat cttgtatttt tctagatttt cagagtaccg
     4201 aatacttttt tgtatcgcaa acgcagaatt atgaactaaa ttgatagata aaaccttttc
     4261 tatgcttaag tatctactat catctttact caaatctact accttcccct tctcatcttt
     4321 aacagtgtta tcgacatcca gaataattac tctggtttcc acaaaactct ccttgtcatt
     4381 gcctttattc tttgtcttgc ttggaataaa tgattgtgat gaatttagta tctctacaaa
     4441 ctcatctctt gtaacttctc tatagttatc taaacaccta tctcttacat cagttttttc
     4501 tttttttgta actggcttct ctaataattt ttctttatct acataaattg gaatattaat
     4561 cattgatgtt tccatttgtg tttcctcctt ataaaattct gttaaagact aaaacataca
     4621 ga
//
LOCUS       DAYYGS010000135         4434 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000135 DAYYGS010000000
VERSION     DAYYGS010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.140"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(543..2048)
                     /locus_tag="ACHA44_002680"
     CDS             complement(543..2048)
                     /locus_tag="ACHA44_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFM8905901.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDPFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="ACHA44_002681"
     CDS             complement(2045..2731)
                     /locus_tag="ACHA44_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8905902.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA44_002682"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="ACHA44_002682"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFM8905903.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1238 a          919 c          730 g         1547 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa agggatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attt
//
LOCUS       DAYYGS010000136         4365 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000136 DAYYGS010000000
VERSION     DAYYGS010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.141"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            169..870
                     /locus_tag="ACHA44_002683"
     CDS             169..870
                     /locus_tag="ACHA44_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HFM8905904.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1442..2458)
                     /gene="tsaD"
                     /locus_tag="ACHA44_002684"
     CDS             complement(1442..2458)
                     /gene="tsaD"
                     /locus_tag="ACHA44_002684"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFM8905905.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2455..2919)
                     /gene="rimI"
                     /locus_tag="ACHA44_002685"
     CDS             complement(2455..2919)
                     /gene="rimI"
                     /locus_tag="ACHA44_002685"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8905906.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2926..3468)
                     /gene="rimI"
                     /locus_tag="ACHA44_002686"
     CDS             complement(2926..3468)
                     /gene="rimI"
                     /locus_tag="ACHA44_002686"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFM8905907.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3452..4174)
                     /gene="tsaB"
                     /locus_tag="ACHA44_002687"
     CDS             complement(3452..4174)
                     /gene="tsaB"
                     /locus_tag="ACHA44_002687"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFM8905908.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
BASE COUNT         1298 a          925 c          744 g         1398 t
ORIGIN      
        1 attcgcttca aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat
       61 atttgtaaag tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt
      121 aaaaaaagtg acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat
      181 aaaatcgatt ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat
      241 gaagagttcc aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc
      301 cttttcgacg atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt
      361 ttagaacgtt atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac
      421 ctttttccta tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg
      481 actgatattg aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat
      541 cccgaacagt tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg
      601 cgtatccaaa tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt
      661 ttgaaagaag aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc
      721 accttaaaag aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc
      781 aaacaactac gtgattccgg actgttagat tatgacaaaa agcgttttac tttctgtaag
      841 acagctacta ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata
      901 tttatcaggg tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag
      961 atcactgggc aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt
     1021 tgcaaaaaat actgatagat taactagagg aagcgtcttg tcctcatccg acctagatta
     1081 ttcgatattc ctcgacaata aacgattaat cagtgagggc aatcagagga acggataatg
     1141 atgaataagc tgtaaaaaat agggctttga caaaagcgtg tcacagccct tatttcgatt
     1201 aaacggtgtg tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag
     1261 ctgttttttg aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga
     1321 ttaaacggcg ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga
     1381 gaagccgttt ttcctgggct gttcttatta cttgatgcag ggcagatttt tcacaacctc
     1441 tttattcaga taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt
     1501 tttgcatttc cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa
     1561 gagggggaat cactagttct acctcaggga gtttagttgc caaagcatcc tgcaatcctt
     1621 cacgcaaccc ttgattggcc gctacacctc cagctacaat cagttgcttg actggataat
     1681 tttgacaagc tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag
     1741 ctgctaaatc atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga
     1801 taaatgcact ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc
     1861 gtggaaaatg gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg
     1921 ggtaagaaag acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc
     1981 ttgtctcccc aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc
     2041 ctccgctaac aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat
     2101 agatatgccc tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag
     2161 ccttagctgc agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag
     2221 cgactgccga taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata
     2281 aggtgatttg ttctacgtga tggcggctag ctacttcagg aactacgcca ccaaaacgtt
     2341 tatgactgtt tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga
     2401 ccgctacact cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt
     2461 catcgtctcc gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata
     2521 atatgatttc cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc
     2581 ttcattagat agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat
     2641 cagtaattgt aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat
     2701 atttgtgatc tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt
     2761 atcttcaact aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat
     2821 tttcgtccaa ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc
     2881 caagtacgct tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa
     2941 aagttttatc atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt
     3001 tgttgcttga aaacctaact tccgataaag tctttgtgca tcttgattac tcatacgcac
     3061 ttctaatgac atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag
     3121 cagcgaaccg attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca
     3181 atctaaaccg aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc
     3241 tagatataga tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg
     3301 caattcgcca ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt
     3361 caattctctt agtacgtaac gacgatcgat ttgttcgatt gttttttctg tataaatcat
     3421 tttatcttga aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc
     3481 caggtgtgtg cgtctccaac catttttctt ctgcttctac ccgttttaaa tagcgaggaa
     3541 gaaaagcttg tttctctgca actggttcag ctaaagcacc taattgagcc acagttacac
     3601 cacttggaat atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta
     3661 tttcatagaa gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt
     3721 gtagttgtgt gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca atttctaatc
     3781 tattttcttt ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg
     3841 gcacaataat tttatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc
     3901 ctactagctc tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa
     3961 gccctgtata agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta
     4021 tttgaagatc tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt
     4081 tgatatttgt ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag
     4141 tttggtttgc agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat
     4201 tctcgccttc cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac
     4261 tagtacaatg atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa
     4321 aaaagctgcg gtatcacttc atttccccac agcttttcca tcttt
//
LOCUS       DAYYGS010000137         4277 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000137 DAYYGS010000000
VERSION     DAYYGS010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.142"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1004)
                     /locus_tag="ACHA44_002688"
     CDS             complement(<1..1004)
                     /locus_tag="ACHA44_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HFM8905909.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     "
     gene            complement(1112..3139)
                     /locus_tag="ACHA44_002689"
     CDS             complement(1112..3139)
                     /locus_tag="ACHA44_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HFM8905910.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(3284..4012)
                     /locus_tag="ACHA44_002690"
     CDS             complement(3284..4012)
                     /locus_tag="ACHA44_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HFM8905911.1"
                     /translation="MKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAG
                     DEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYAA
                     ADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVS
                     LVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVP
                     GKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1177 a          894 c          706 g         1500 t
ORIGIN      
        1 caattattgt cttttttaat cgtaacatcc gtcgtgttca attccttggc tacttttccg
       61 tctattgtaa aatctttttc aaatacccat tgattggtat agttgatcgt agtatttcct
      121 ttttttctcg aaaattttcc attttcattt tcatttccta aaacagtcat tgtaaggtga
      181 tattcacctg gggttagcgg ttgaccattt aaagcagttg gaaaagaaaa atttgtattc
      241 ggcgcaatct gcaatccttc tttttcctct tgatataaaa cttcagaatg tccttttttt
      301 gtaatttttg tttcaataac tacttgattg atataggttt tttgatcatt ttgaacgttt
      361 gctaagatga catttcttgc attgatttga tctggtttaa cttcatgaag agtaaggttc
      421 ggagcaactt cgttcagtgt ctgttgcatc aacaaagcaa taatataggt gtattcattc
      481 tcaatcgata agctttgatc cttagtttct gtttggtcct tttccgtctg gatttcttgg
      541 aatgtgatcc caccagccaa tacaccatca aatcgctcat tcggcatcct gacatgaaaa
      601 gcaacggttt ggacactttt aggttttagc gtcactatct gtggatagct cacataatct
      661 accagatcaa agatcaggct catgtctttt tcaatctcgt tttttccgta ttcaaccatc
      721 acatttgaat tggtggttgc gctattcaca gaaatatcga ttttcacttc tttttctgta
      781 tcatttctca attccgcttt tacttctact tcctgatccg gatcaagttg aagatcgaag
      841 taagtttttc tcttgtcgat ttgattctct gaagttatcg gcgtcactga aaaattaaat
      901 gcactagctg acactgatat attgttaaaa agtgccccta aaataactag gagtgttata
      961 aataattgtt gttttggtct caagaaaatc gctcctttaa tcatatgtaa gagaagacta
     1021 taacaaaatc ttgtctctgt gggacacaag agcctatggt acctgtgccc ttcttgaaac
     1081 tcaataattt tgttatattc ttctcttcct gttaatttcc tgggacatct gcaagtatcc
     1141 aagatagttt cccctcaaag gttccgattc tttggttttc tacaggaata tttattctga
     1201 ttccacgatg agctgtagaa tctaaaagag aggacaaatc gctttcaccc ttttgatcac
     1261 tttcgaaaat cgtaacagcg gatgtcccta taaccgttgt attttcagag gtaacaaagg
     1321 aaagagcttc tggaagctca aaatctccat ttgtaagaac tttctcttgt ttcaattgta
     1381 atttccaact tccaaaagcc cgttcatcta taattttcaa cccacctgaa aactgtccga
     1441 attcacttaa tggtctactg gatattttat gttttccaaa attgacatat tgaggagcag
     1501 ttgagagata aagtttgcct tttctgtaat aaaaatagat atcctctgta ttttcggaga
     1561 agatgccttc tgtattcgct gaatccgagt caaaaacata cccttcaata tcaataggag
     1621 tttctttata cttctctcct attttcccag tgattgtttt catttcagca atatcaatct
     1681 gactgtcttt atctttataa taaacatgga tatctatctc cttcagcttt gcgatcttta
     1741 ggtttatttc attcgattgc tcaatcaaat tcccgttttc aaggtattca aaatgcatag
     1801 tgagtacttt ttcaggaatt acttcctgca aagttgtctc caattcgatt attaacgtat
     1861 ctccagcaat aaaataatct gtcagattta gcgtgactcg atttccggtt atcaagatat
     1921 ctttggcatc aatttgattt ccattgatca agatatgctc gggaatagtt aattcttgtg
     1981 ggaggtcgat actagtctgc attttcttat attcaagaaa atccccctcg accttcaatg
     2041 tatttctgat agttatctta tcgtttcctc cagcttcagc gatggcccca taatcactat
     2101 cgttgacttt tacatcagct gttgatgtga cattcgatgg aagtcgcgtg acagcgacag
     2161 cattttcttg aaaaaaagta cctgttgaag aagtaaaccc ccaatttact tctgtagaac
     2221 caaactgcct aatgagatca ggtataatcc gagaagagcg accgacacct tccaaatcat
     2281 aagtaagact tctttgctcc gcgtcccatt taattattaa caagcgccat gtgccattag
     2341 aaagaaatgt aggagctacc gtcacgccag tatgctctcc agaataatta ttattatttg
     2401 gtttaggcgt gacaaaagcg acatgtccat gccctctatt atcagcagtt atctctcgat
     2461 ccattcgatc agaagaacca ctatttttat acgtatcaaa ctcaatcgag agcgcattcg
     2521 caatgtaagg ttggccagaa cgagtagaat atgagccaat tcccatacct ggcgatccaa
     2581 taacttgact gggattacta gccattctag gatcgccagt caatgtaaag gtcatgccat
     2641 ctcccgctct atctcgttga tttcctaagt ataaataaga tttaaatgta aaatccgaga
     2701 gtaaactaat cttcttattt gaccaaatag ctcctctttg agacctttct gctgggttca
     2761 actgcagtaa cttcccattt tccaatatgg acgtgctagt accgataggt gtttgaaaaa
     2821 ttttatcgat agaaataata ttgggatcgt caggcagctc aaccgcagaa attggtgcat
     2881 cttctagtcc ttgtttgtcc gaaccctcag cttctacgtt gtcagtggtc tctccactac
     2941 tcgaaatact taaatcctca ctgctttctt gtgtctggtt ggattctatt aaactgtcga
     3001 atgtcgaagt gagtttgctt tcttcgtctc ttctatttcc atgctccaac tgattcaatt
     3061 ccaaagagta ccctatgatc ggaaagatat tagtcactaa gagactgctt aaaatgagca
     3121 cgatcctcct ctttttcaat atatcttttc ctcctgatcg ttaaattcct agatgaacta
     3181 ttcgtatcca aacaaattta gcctagcctt tgacaatttt tgctcgatta cctcgcaaga
     3241 caatatgact acgtatttat ttttaacagt tcttttcagt aaactagttg ttaggtgtat
     3301 cttctaatgt ccaaactaat ttagtggaat attgttgcgc taacttgaca gatttaccgg
     3361 gtaccgaaag ctgtaccgca tcttgatttt cattttcatt tgcaccaaat cgatagaccc
     3421 aagtccccac acctgttcct gatttagcat taattgctaa cgtattgttt cctcccggtg
     3481 tcaacgttaa ttcagaattc accgtttgcg gcgtatatgt tggatcaacc aaagaaacag
     3541 cctgcccttt cgtaaatttc agctgtgctc caactagttc atccccactg gcagttttaa
     3601 actgttctgg ttgagagaca cttaatttcc acccagataa ggtacttctc ctatctgtca
     3661 cttggacata tgtcggtttt ctagacccat cgttcatcac atcagcagct gcataatacg
     3721 ttttatccgc agtagaaatt ttttgagtcc caaatttgaa ttttgatcca taatcaatcg
     3781 ataatgcccc tcctgttcca gggcttggag gatctgatgg atcgacaggt ttattgggat
     3841 caggattctc tggatcaaca ggaggagtta caccctcatc tcctgcttca aatgtaatcg
     3901 ttccttctgt gttatattcc tttgggtata cttcagttgc agaaacaaaa gtcgtacttg
     3961 ctaccatgct gaataacatc attgctgctg ttaatacgat ttcttttttc atcattgaca
     4021 ttcctctttc tttatttact aaacatatca gtcgattgga ttaatccata gcaaaattct
     4081 gttttacgat acttatgaaa taaaagaaca tagacctata tttggtccta tgtatcgatt
     4141 agttataaat gattatcagc ccgatttgat ttctagtttt tattgcaact tgatgcaaac
     4201 acaatctaga aatacagctg tcatagcctt taaaataatt cgtaagtccc cctcctatct
     4261 atcaaaatgt gacttga
//
LOCUS       DAYYGS010000138         4205 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000138 DAYYGS010000000
VERSION     DAYYGS010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.143"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            994..1962
                     /locus_tag="ACHA44_002691"
     CDS             994..1962
                     /locus_tag="ACHA44_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8905912.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1979..2503
                     /locus_tag="ACHA44_002692"
     CDS             1979..2503
                     /locus_tag="ACHA44_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8905913.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            2879..3160
                     /locus_tag="ACHA44_002693"
     CDS             2879..3160
                     /locus_tag="ACHA44_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905914.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            3162..3578
                     /locus_tag="ACHA44_002694"
     CDS             3162..3578
                     /locus_tag="ACHA44_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905915.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1605 a          736 c          693 g         1171 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caaacacttt agtgagtttt gtagaaagta
       61 cctttaaagg tactttcaca ttacgaacga tacgtccgta ggacgtactg aaagggtggg
      121 atttatgagc gaactaatgc aaaatttggc tagcaaaaaa agttaaaaaa tatatgctaa
      181 taatcgagcg aaatgaacac tttttcaagt gagcgaatcc gccatgacaa agctgaagca
      241 gtttatttca gctttgtcat ggcgtgtgcc tagactttta aaaaagtcgg cacacgagca
      301 aatatcttta tcaagctcta atttgcgttt taagacgttt gaaacgtcta agtatataaa
      361 tatataagag taggtttaag agtgcttaaa tcgaatttta gagggcattc agacaacttt
      421 agggttgtta gggcttgccc tgaccgcctg taaggcgctt taagaacaag ataagggatt
      481 tatactaaga ctgtcttaaa acgtctcaaa cgaaggctca tagcttctca aagagttcct
      541 tatgaacatt tttagcaaag gagccggaag cgactgcgca gtataatgtg agagcagagc
      601 gatcacacct ttttaagcga ctgtagggag taagattccc agttgagaaa aatgcgcgaa
      661 tctgtttgga gaaaaaagaa gtggtttttt aggcgggaaa ttttgtcccc ctcttttata
      721 atctcttttt aaacctcttt ttaaacctct ttaccgtcgg ctatatcctt actctcccaa
      781 gggaatgacg cttttatcta catcgttttg tctgtttatc tacatcgttt tgtctgttta
      841 tctacatcgt tttgtctgtt tatctacatc gttttgtctg tttgtacatt gaattagtac
      901 atcgacgatg ttaatataag atgtagataa acaggcattt tgtatttcat aacgtgtatc
      961 tcttgtgcga gtaagaaagg agctatttta aatatgtcta atgaaatcgt aaagtatcat
     1021 catgaattaa atacaattcc gttgcgaaaa tttacaccag tcgaaatgaa tttgttcttt
     1081 tctatcgtat caagaatgcg tgatgtaggt gataaaaaag tacaatttac attcgagcaa
     1141 ttaaaagatt taagcaatta taaagcaact gcaaatgttc gattcataga tgatttagaa
     1201 acaacgtatg acaaattaat ggatttacgt tttggtagaa gaagtgctga cggtttgcaa
     1261 cgtgaacgtt ttgttttatt taatcaattt aaaatcgacg gaaaatctga tattcctttt
     1321 gctgaaatac aagttcatga aaaagcctta cccttactta ataatcttga agaatgggtg
     1381 cgctattctc tccaacaatt caatgaatta gaaagcagtt attcaaaaac aatgtttcga
     1441 ttactaaaac aatacagaac caaaggtttc gcttattttt caaaagaaga ctttcatgaa
     1501 ttacttgata ttcctaaaag ttacaaccaa ggtacgattg atcaacgtgt gttcaatcca
     1561 atacgacaag aactaaccgc aatctttaaa ggtttaacaa tcaaaaagaa atacggaaaa
     1621 ggacggggaa agcctgtcat tggctatcaa tttacattta aacctgagat aaaaaaatca
     1681 gacgacttta acaaacatgc cccaacaaaa gaagaacaaa acaaagcatt agaactagct
     1741 aaccaacaag ttcaatctga aaaagcacct acttcattac aagaaaaaat caaagaacaa
     1801 aagcgaaaat tggaactttt acaacagtta gaaaagttag aaagagaaca aaatacccaa
     1861 aataaagaaa ataatgcaca aaaatcagag ataaattccg atattccttc agaactaaaa
     1921 aaagacttat tagacaatct tcaaaactta ttcaaagatt gaggggtttt tagattttat
     1981 gacagaaaaa acaatcaaag aacttgctga agaattagga gttagtaaaa cagcaattaa
     2041 caaaaaagtt tctgacgaaa atcgaaaact ttggttttcg aaaatcggaa acaagtttgt
     2101 gattaatgaa accggacaaa aggctataaa atcaatgttt tttaacgaaa acgaaaactc
     2161 aaatcaacaa ctagttggcg aaaaaacgaa aaccgaaaac cacaaaaacg ccaaccaagt
     2221 tcccgaatta gaagtccttt ctctcctaga acaacagtta aatcaaaaag atatgcaact
     2281 acaagaaaaa gacgagcaaa taaaacaaat gcaaaaatta ctcgaccacc aacaacaact
     2341 caccctgcaa gccaaccaac aaatcgagaa actgcaggaa caactacagt tgacttatac
     2401 ggaagaatct tcggaaaata aatcaacgac tctttctgaa gaaacagaat cgatagaaaa
     2461 acaatcaaag gcggagaaaa aatggtggca attctggcaa taaaaccagc aatttaaatt
     2521 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
     2581 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
     2641 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
     2701 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
     2761 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
     2821 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
     2881 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
     2941 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
     3001 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
     3061 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
     3121 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
     3181 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
     3241 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
     3301 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
     3361 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
     3421 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
     3481 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     3541 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     3601 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     3661 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttggggcgct
     3721 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     3781 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     3841 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     3901 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     3961 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     4021 gtgagcgtct ctatccattg gtagaggggc tacttggtct agtcgacatt atgttaacta
     4081 gctcgataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     4141 ttatcgcata ttgtatattt taattatata attataggtg tataccactt aaatttaaca
     4201 caaag
//
LOCUS       DAYYGS010000139         4188 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000139 DAYYGS010000000
VERSION     DAYYGS010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4188
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.144"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            41..340
                     /locus_tag="ACHA44_002695"
     CDS             41..340
                     /locus_tag="ACHA44_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905916.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            605..1480
                     /locus_tag="ACHA44_002696"
     CDS             605..1480
                     /locus_tag="ACHA44_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905917.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            1477..1695
                     /locus_tag="ACHA44_002697"
     CDS             1477..1695
                     /locus_tag="ACHA44_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905918.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            1682..>4188
                     /locus_tag="ACHA44_002698"
     CDS             1682..>4188
                     /locus_tag="ACHA44_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pLS20_p028 family conjugation system
                     transmembrane protein"
                     /protein_id="HFM8905919.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDS"
BASE COUNT         1609 a          520 c          862 g         1197 t
ORIGIN      
        1 gaggagttat agaataatta attaaaaagg agaggttttt gtggaagcaa taaaattaat
       61 gatttcagaa gaacaggagg aaatgtttct aaagcagttt tatcaaattg ctacaaaagc
      121 tattgaaaag gctcaagaaa atgcggggat gacaagggaa tatctcaatc aaaaagaaat
      181 ggcggaatac ttaaatgtga gtgaaaactt tttaaaaaat gctgtgagaa atgagggatt
      241 gcccgtagtt gaattagggg caagaaaatt ttattcgaaa aaggcagtta atgagtttat
      301 gtttgctcga atgaaaagag gggaaaatat tggaagatga tgaagtagaa attataaaaa
      361 aatgctttaa aaaaagaaaa aaagtgttcc caaaagtttg cagactgggg gaacactcaa
      421 taataaagtg ctgagaaaac tttatttata gctattatat caaaaaagaa ggtgaaaagg
      481 tgttttcttc tggaaatgtt gtagttgaca aacttagaga aataaatttt gacggaaatg
      541 taatccacac acatggtacg atcaattaag aaaaaaaacg aaaaaaggtg ttgagaagcc
      601 ctatttgaat gcaatagtta tcttagcaga aattacctat tggtatagac ctaaaaaaat
      661 ttttaacgat gatggtcagc tacttggtta tcaaaaaaag tttcttgaag atattttgca
      721 aaaatcatat aagcaattaa gcaaaaaaac aggattatca gaacgagcta taaaagatgc
      781 aattgtgtac ttagagaaaa aggggattat caaaagggtt tttagaaata tttattatga
      841 gaaaagagca gttacattat caaatgttat gtacattgtt ttaaatgtac ataaattgat
      901 agaaattact tttcctactt tggattgcga aaaaaaggag tatatgccga aaattcgtca
      961 gaggggtatc gaaaaaaaga gatatgtctc tttaaaaaaa ggtagaggta tgtacaaatt
     1021 tgtatatacg tatacaaaga ctacttcaca gaacatatca aagactactc tcgttatgga
     1081 agaagaggat attaatataa agaggattta cagtaagatt agtgttcttt atgacgcaaa
     1141 taacgaagat ggtcgaaaat tatttcaagt gtatcactat ttgagaaacg ctaatgtaga
     1201 tacagaagat atcagaaaaa tcattgaata tttatctgaa aatactcagt atcttattgc
     1261 tgagtgtatt ataaaacaac atgaagcatg cttttttcaa agaaaaactg aagaagggtt
     1321 atacgattat gtaacatatt ttttgaatgg acttcagaaa agatataaaa gtcaattttt
     1381 agaaaatgta gagcctatag atcagttcaa aaaagctttc gaaatcaaag agttacctga
     1441 aattactatg cataattggt tgaaaggaga agaatcatga ttaaaaatcg cttaatagat
     1501 caaatgtggt gtcttgatca aaatacagat actctcgaat taatcgagag tatctgcttc
     1561 aacacagtag ttctcgattt aagagctgaa aatgacaatt gtgtaactca tatcataatt
     1621 aatcaaaaaa tggctcagca gctctctgag agcctaagaa aatgggctga aggagaaaac
     1681 tatgaaagca attaaagaaa aaatcgttag tagaaaaata aactggttag cactcagtgt
     1741 acttgtttta atgattagcg ttgtttttac aagtaatatt gttgaagcta gcattttcaa
     1801 ttggtttgat gacacaaaaa gtcaaacaga gtttttgaat aattctcttt atagacctta
     1861 cctaaaaaaa tacaatggag atttccttag tcagttcggg atttggcttg gatgggctgt
     1921 ggtaaaaggc atgtttactg tgactgattc gattcaaaat atgattccag atgtgttgga
     1981 tttgttcaat ttcattgaaa gtacagggct aaataatgtt tatcaatcag tcataaatac
     2041 aattgtcgtt ggattaatga tcctttcatt gatgtttgtt ggctacaaga tgattactgg
     2101 aaaaggaaca atagatttaa gatctgttgg aatgaatata gtgatgtctg tagcgttaat
     2161 tcttttaatg ccaacaatga tttctagcgg tattcaattt gccaaaatat tttacaatga
     2221 tgctactacg attacaaatt cagatgacgg agtcgcttgg tcattgataa aacaaggagt
     2281 aacagatctg gcatatatta ataaaacaga tcaatactca agcattgata aaacggagga
     2341 tcgaaataag ttaacacgaa aaaattttca acagacagat ttaacacaag tgttgacgga
     2401 taaagtgatt gataaattgg aaaaagaaaa tccagctgcg gataatcttc gctatgaatt
     2461 agttgaaaat tctaataatg aatttgttgc cacaaagttt tctgataact tcttatcaac
     2521 attttctgat agcttgaaat caggttatta tcgatatcaa gcaaatcttt gggggatttc
     2581 gattgggttg acagcgttgg caatcgccta tgtgttcagc gcgtttgtga tcattacagc
     2641 aatattagaa cttgcattta aacgtgtgtt aggtgttctt gtttttgcga cagatattga
     2701 aacaggacaa cgttcaaaag ttgttttatc cgatatttta caatgttact taacagttgg
     2761 atttcaaggg tttggactat ctatgtttgc tatgtttatc aattttttaa atgctggaca
     2821 aggtatctcc acaaatattt ttataaaaac aattgcttat atctgtgctg tgtttgtgct
     2881 gataaaaggc agtggaacag tcatgagata ctttggtgtt gatattggac tcaaagaagg
     2941 ctatgggcaa cttgcttctg cttttggtat gggcgcaatg ctgtttagaa aaggatcaac
     3001 tggatttaat cgtgctaaag gaagcggaaa tggaaatgga tcagacagtg gagaaggaga
     3061 agatcgaaaa ccagaaaaga atttcggcga aactctatca aaaaaagctg gcaaaactgg
     3121 aagagctttg ggatatgccc atgaacgtgg attatcaggt ttagcttcag atggagctac
     3181 aatggctagt gaaagagcaa taaaaccatt taagagtatg cgtgatatgg ctaatgatac
     3241 caagaataag ttcaaagaag gtttagatga tggtacagtg tccgctatta ataaaaatag
     3301 caaacctatg ttagctaaaa ataaagaaga tgaaactgga aaatatgctg attccatgcc
     3361 aacacgtctt tcggatagaa aagatggtgc taaggttgaa aatgccgata gaattatgtc
     3421 tagttctgaa cgtatgcgtg aagcaatgaa aaataatgct gaatcgaata ataatcctgt
     3481 aagtgcaatt cagcaaaaag ttcaacaaga tattgaagaa cgtaaaaatg ctatgcacgg
     3541 tcaagctaag tctgctgaag aattaattaa tcagaagcga caagaagcta aatacacgcc
     3601 tgaagcaatg aatcgtgagg aaatgttaag gaaacgtgta gaagggcgta caggagctaa
     3661 tacggacgaa aaagaagcgt tgaccaaaga aagaatccaa gaagctaaaa actcaaatac
     3721 cggattagaa aaattggtca aagaaaatct acaaaattct acaagtagtc aaggtggccg
     3781 atcagtagat gtccgagaaa atattcaagg ttcattgaat ggtcatgtcg gacggaatgt
     3841 tgatgtaaga gagaatttac aaagctcttc aaatggtcaa ggtactaaga caattgatgt
     3901 tcgagaaaat gtacaaaatt ctacaagtag tcaagggttt aaatcaatgg atgtaagaga
     3961 gaatgtacaa aattctacaa gtagtcaagg gtttaaatca atggatgtaa gagagaatgt
     4021 acaaagttct acaagtagtc aaggattcaa aacagtagat gttcgggaga acgttcaaaa
     4081 agatagtgga gaaatgaaag aaaaaacaca aaaaatcaat atcgttgaag acagaaagtc
     4141 agcgaaaaaa tttgattcaa atcatgaaac agtgattatt gatagtga
//
LOCUS       DAYYGS010000140         4158 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000140 DAYYGS010000000
VERSION     DAYYGS010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.145"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            186..2855
                     /locus_tag="ACHA44_002699"
     CDS             186..2855
                     /locus_tag="ACHA44_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HFM8905920.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3560)
                     /locus_tag="ACHA44_002700"
     CDS             complement(2946..3560)
                     /locus_tag="ACHA44_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8905921.1"
                     /translation="MINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKAMH
                     MVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPGIE
                     KRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTIIK
                     ITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3681..>4158
                     /locus_tag="ACHA44_002701"
     CDS             3681..>4158
                     /locus_tag="ACHA44_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905922.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGEMYFTFTGKRQYYSVKLAMQV"
BASE COUNT         1265 a          739 c          772 g         1382 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aaataacggt
     3601 gtacattttt aaaaggagat gtataatcga gatttagaaa gcgttttcct caaaatttaa
     3661 tgaattagga agtgattact atgaaaaaat tatttagcgc actttttatt ctgttatttg
     3721 gctttattgg tattgggact tctggaaata tagttaaagc tagcacgatg gttgaacaaa
     3781 caagtgaaat ggcaaaaact tttagtcaat taacaccaga agaacaaagt tacttcaaat
     3841 ccaaaggatt tagtgataaa gatacttttt acactcaaaa gatagagcct caagatccga
     3901 aaacacgttt ttctgcaaat gtattgcggt taaccgcatc tactaaaaaa attactaata
     3961 cgcaggggcg gacagaatat attataacaa gtcccaattc ggcgatgtat cgtatttcta
     4021 cacgcttgag tttaggtagt gcaaaatcat atactcaaac agtaaatcta gctaattctt
     4081 atggttatgg tggagaaatg tattttacat ttactgggaa aagacaatat tacagtgtaa
     4141 aattagctat gcaagttt
//
LOCUS       DAYYGS010000141         4109 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000141 DAYYGS010000000
VERSION     DAYYGS010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.146"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            47..1348
                     /locus_tag="ACHA44_002702"
     CDS             47..1348
                     /locus_tag="ACHA44_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8905923.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1375..1635
                     /locus_tag="ACHA44_002703"
     CDS             1375..1635
                     /locus_tag="ACHA44_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFM8905924.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2634..3869)
                     /locus_tag="ACHA44_002704"
     CDS             complement(2634..3869)
                     /locus_tag="ACHA44_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFM8905925.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1173 a          664 c          860 g         1412 t
ORIGIN      
        1 gtcatgcaat gaatggttca tattttattg aaaaggagtg gttttagtgg aaaagctgat
       61 gaaatgggtc gaagagaaac ttgtcccacc aatggcaaaa attggtacac agcgtcattt
      121 attagcaatt cgtaatgggg ttgtttcaac attgtcattg attttgattg ggacattttt
      181 tatggtcttt atcaatttac cgtttcctgg atggaatgaa tttattgccc cttattcggc
      241 aacgattgtt ttacccttta ggatgacgat gggattgatg gctatttatg caacttttgt
      301 tatgggttcg gatctagcta aatcatatgg attagatagt gtgacaggtg gtatattatc
      361 tttaggaacg ttctttatgt tacaagttcc tgtaaacgtt ttaactcctg aagaggcacc
      421 tcttggctgg gtcttaccta tgagttcttt gggagcatct gggatgtttg cggggatctt
      481 atctatgatt tttgcagtgg aagtatatcg tttctttaaa aatcgaaata ttactatcaa
      541 aatgccagaa caagtgccac ctgctgttgc ccgtagtttt gaggcattga ttccaggtgc
      601 agtcatttta acaattactt ggctgattcg aagcgtcgcg ggctttgatg taaacgctgc
      661 cttattatcg ttattcgagc ctttaaccaa tattttaggg aataatttgc ttggtgtttt
      721 actaccaatg tttttgatcc atttgttgtg gtcatttggt atccatggca tgagtattgt
      781 aggggctgtt gttcgtccaa tgtggctgat catgttggat gagaatgcaa aggcattggc
      841 agatggtaca gctgccaccc aattacccta tatcgctcct gaacagtttt atcaatggac
      901 ggtaacaatt ggtggtgcag gagcaacgat cgtggtatct gttttgttta tattcttctg
      961 taaatcaaaa ttcttgaaag aggttggtcg tttttctatt attcctggta tcttcaatat
     1021 caatgagccc ataatttttg gagctcctat gatgttgaat ccttacatgt tcatcccgtt
     1081 caacttagtt cctttagttt tgactattat cagttatttt gcagtcaaat tagaaatggt
     1141 caatagattt actgttttgc caacatggac attaccagct ccaatcggcg gttatctttc
     1201 agcaggaaat gactggcgtg ttgttatctt gatcatgatc aacacattga tagcatttat
     1261 tatctattat ccatttgtaa aagcttatga taaaaagatg ctgtcagatg aacaaagtcg
     1321 tggagaaaac gaagtccaga ctgtctaagt ttggttttta gaaggtggga aacaatgtat
     1381 tggctgattt tattttttgt ttttatcttt ttattaacgg ctagtcacct tatattgaat
     1441 atgctagcta catatcatat tcaaatcaat cgatggattt gggcactggc atcatttttg
     1501 atcgtgattc ttccaaagat cattgtaccg catatgaatg ttttgttttc atgggggaca
     1561 tatgtacttt gtggaatttt tgctatcaat tttatgatcg agcagcatag atggtttgtc
     1621 acatcgaagc tttaaatgtt ctaaaagtgg ttgtaacaga agtggttagc ttcaagaaat
     1681 aagaagaaat ttacgaaaat tgcttcttaa atttttgtga aatgttggct tatttttcgt
     1741 ttggttgaac ttattaccgc agatgcaagc ttttgaccac cgtttattcg gaatatggga
     1801 ctgtgacaag gcttttgtca cagccctttt ttacataaag attattattg ttttaactgt
     1861 tttggcagaa agaaggagga aaaaatggaa attttatcag caatggattt agaattagta
     1921 gatctactac caaagtgatc aagtcaaata tagatttggg agcttacaac catatagtag
     1981 gggctgcagt atggtgtatg aatggtaata tgtatgatgt gagtgtttat tcaaatcatg
     2041 aaacgtgtat agaaataatt gtgctgggaa tagcaatcag tcatggtgaa cgtgactttt
     2101 agtgtatcgt tgctgacgac atagaacatt ctgacatgat ctactcaata tgtggggatt
     2161 gctggcagtt tatataggag tatgcatcgg attgcgagat cattgtctca acagaagagg
     2221 gaggttgatt atcaaagata aagtggtaaa tcctacattt gatttagtaa ttgttgaggg
     2281 gactagcggc acaaatctct acaaagcgat gcttctagat ctatatgaca cattttaaga
     2341 aaatgtattt gcctaattat tatggtcttt cttttgaaga aacgttgaga tgatccaacc
     2401 agagaaaggt gtcaaagaat ttgactaaga ataaatggca gacgctacct atgctcattt
     2461 ggatagcgtc agatctttta ttttaaggat gattttgtgt tttttagaca ccgtaattta
     2521 agtaactcta acttttcaat ccactattta ctttttttgt attttcactg tctgggtctc
     2581 gaatagcgaa attactgatg agtctctatt aaaaaagctt tttctacttg aaactatatt
     2641 gggtggttgc tcaggaaggt ttcgatttct tgaattgctt gttgtggacc atttattgga
     2701 aaaacggctt gcagcgtgcg tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga
     2761 gcaatctgca gttttctttc agtcactggt tttgtgaggg cgattgcgtt tccgtatttt
     2821 acacaatagt caatattaca gctttgctca aattgcatcc ccacaccgat aaacggtttg
     2881 ccggattcac aggcagtttg gacggtccct tccccgccgt ggataaaaga aaaatcaatg
     2941 aattcatgca aaagatgact agacagatat tctgttatta aaagatcttc tgagtcagat
     3001 tgaatgtcat caagcagata gtcaataggt gcaataaccg ctagatccgg ttgtttttgt
     3061 aagaagaaca aaattttgcg taacatacgc ggattgcctg aactacccat tgcgaaatag
     3121 ataatacgtt ttctttcttg ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag
     3181 gaagtactga gcttagcaaa aattggacca acgattcgat aattttccgg cagctgttct
     3241 tcttccaaaa atagtttagg cgttgtgatg agatttagat cagcatccat catttgtaaa
     3301 cttgtttttg gtaaaggaag catttctttt tgtgcagcgt gtttgaaggc attggggatc
     3361 catcgggtat ttactatacg cttttttgtc cagtgccaaa ggggattgct taaagggccg
     3421 aagaaacgta actcgtgggg catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa
     3481 gcatagggtt taacatatac taaaggaatc tttttattgc gggccgataa aaaaattgtc
     3541 acattcgaac ctgtcacaat cagtcgaggc tcaaacttag caatcagact ctgttcacta
     3601 gtgatacgag aggcaaccat ttctttggta aaagggtttt taagtgtacg caactggtca
     3661 aattttatga tttgttcttc ttgttttttc gtaaggatcg gcgctagtaa atgatactca
     3721 aagccacttt ctgtgatcaa aggagcaaaa gttttagaaa agccaaagaa tacacagtaa
     3781 taacggtctt tcatagcttt ggcaacctca atcattcgtg ttgtctcagc taaattgaat
     3841 actgcaggag caaaaagaat tgtcttcatg aaatcctcct tattggaatg agtctatccc
     3901 ttcgtttctt actagtatac taaaaaacac agcgttcgag tagcctatgt atattttgaa
     3961 tattaaactg tctctcgctg ttttcacaac ttctaaattt aaattttgta atcaagttga
     4021 aactccaatt ttcaaaaaaa cacgcaacaa ttttttgatc gatttatatg ttgtgccaga
     4081 taaatacgag aagttgtgtc attaaaaat
//
LOCUS       DAYYGS010000142         4066 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000142 DAYYGS010000000
VERSION     DAYYGS010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.147"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            85..1434
                     /locus_tag="ACHA44_002705"
     CDS             85..1434
                     /locus_tag="ACHA44_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8905926.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="ACHA44_002706"
     CDS             complement(1562..2152)
                     /locus_tag="ACHA44_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HFM8905927.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA44_002707"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="ACHA44_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HFM8905928.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DAYYGS010000143         4065 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000143 DAYYGS010000000
VERSION     DAYYGS010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.148"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1155)
                     /locus_tag="ACHA44_002708"
     CDS             complement(<1..1155)
                     /locus_tag="ACHA44_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8905929.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
     gene            complement(1158..3407)
                     /locus_tag="ACHA44_002709"
     CDS             complement(1158..3407)
                     /locus_tag="ACHA44_002709"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFM8905930.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKISEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            complement(3504..4007)
                     /locus_tag="ACHA44_002710"
     CDS             complement(3504..4007)
                     /locus_tag="ACHA44_002710"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905931.1"
                     /translation="MENSIYLVTEPNTQLKDQDNLVSELSRQKQVYKFLELLLNSASY
                     LNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISIK
                     EYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRLG
                     LGIINGE"
BASE COUNT         1310 a          743 c          580 g         1432 t
ORIGIN      
        1 tctagtattt tttttatttg tcgcaaaaaa tgttacaaat aggatcacaa taaatattcc
       61 tgcgaataca aaaaatggag cagtcaaatt tgtacttctt gttagaaatt gtgctaattg
      121 catagcaata ggagctataa aattaccaat attacagcca ataaaaatca atgaagtagc
      181 aaaattaatt gtattctttg tagtgacttt atctaagtta ctaaagataa atgggaagca
      241 ccatgatcct gttatacttg ataaaaataa acccgttagc aggaacgtca tattaccgtt
      301 tgacacagca attaacaaat ttgaaattat aaagaatata attcccaagt atagggttcc
      361 ttttcctatc cacttattta ttggtccaaa cataaatcct gctagtattc ctaagatagg
      421 cattagagct aaatagttac tagcattaaa gtttacccct ttaatttcag ttgcaattga
      481 tgcaaatcgt acagaaatag caatactatt cattacgagt aagattgcaa atagaactag
      541 ggcaaaaacc attggattca tcttttcttt tacatgattt tcaggtatgt cactattttc
      601 atctctaatt tcaggaactt ttagcgcgaa taagagagct acaggaaacg caattgcata
      661 gactaaaaac gaagccgtcc aattaatgtt cactaaaact ccagcaagaa aaataaggat
      721 tgtctgacct aaagcttcca ttgaatttct aatacctaat agcgttgctc tagtattgcc
      781 agagtataaa gagctgatat atgtcacagc caatgaatta tatactccta aaccggcacc
      841 taatattact cgactaataa gaacggtttg gaaggtagct gtaaatactg gaataattcc
      901 tcctatacca gctaataata aacctaaaat tattgtcttt ttcattccta ttcttttggc
      961 aaagtaacta gataataaga taaacaaaat aacagttata cttggtgcag ttcctaaaag
     1021 ttcactttga ctttgtgata tccccaaaga ctgtttcatt tgtggaataa caccattaat
     1081 tgcttgagag cttgttaaca ttagtgaaat acttaagatt gctccttttt gaagtaagct
     1141 tttttggtta ttcatctcta cttttcctcc tgtgttataa cccaaatctt ggaatcaaaa
     1201 tcaaatgttt ctgggcgtcg attctgatca tttatttgtc cactagatgc atctgaacag
     1261 actagtcctg ctctcatcaa ttccattcca gatcgcaaac tattttgtcc tacattataa
     1321 agtccgtcat tatctaaccc ttttaatttc agtcggcggt aaggagcatt tacctcattt
     1381 aatgttttat aatgagcaac aatagccgtt ttcttatcat gactaacaac catccacgcg
     1441 gtttgattat catcatcgaa tggtgataat agtctataaa aagtaccatt atgaattatg
     1501 gttctatatt ttttataaaa ggcaatttgt tcttttacaa tcctcaaatc gccttcactt
     1561 aattcattta agtcaagctc atatccaaat gcaccaaaaa aagctacatt tcctctagtt
     1621 tttataggtg ttatacggtt tgtttgatga tttggaacaa ttgatacatg agcaccaata
     1681 gacgaaagag gatacagcat actagttcca tattgaattt ttaacctttc aattgcatct
     1741 gaatcatcac tagcccaagc ttgaggtgca tagtgaagca ttccaggatc aaagcgccca
     1801 ccacctgatg aacaagactc aaatagtatg ttaggatatt ttgttataag tttttcatat
     1861 aagcgatata cgcctagtac atatctgtgg aatacttctc cttgttgatc tgctgacaag
     1921 tgactgtcaa atacatcagt caatggacga ttcatatccc acttaatgta atctaagttt
     1981 gattcatcaa tgattttaca catttgatcg aaaatatttt caacaacctc atctaaccca
     2041 aaatttaata catattggtt cctaccatga ttaggttttc gattaggtgt agaaataatc
     2101 caattcggat gtgtgcgata taactcacta tccttattca ccatttcagg ttcaaaccat
     2161 aaaccaaatt tcataccaaa actacgaatt ttttctgaaa ggctgccgat tcctctaggt
     2221 agtcttttag gatttacatg ccaatctcct aatcctgcac aatcgttttc tctttctcca
     2281 aaccagccat catctaatac aaataattca ataccaacat caaatgcttt tttagcaata
     2341 tttaccaact tttcttcgtc aaaatcaaaa tatgttgctt cccaattatt aattaagaca
     2401 ggacggtctt tttctcgcca cttaccacga gctaagcgtt ttctaaatag ttgatgaaaa
     2461 gtttgactca tggcatttaa acccttactt gaataaacca gaatcgcttc tggtgctgta
     2521 aatatttctc ctggattgag tttccaatta aatccaaatg gattgatgcc aacttgtagt
     2581 ctagtaacat cccatgtatc tacttctgct tgaattaaga agtttcctga gtaaactagt
     2641 gctgcaccaa tagcttctcc ttgattttca tcagtatttt ttctttttaa agtaacaaat
     2701 gggttatgct gatggcttga aatacctcga gtagattcga ttgattgaat tccttgttgt
     2761 aaagtacgcg acttaatagt tctttctcta ccccaagcac cagataattg ttgccattca
     2821 taatttgcat cgggtaaatc caatgataaa ctgctaatat tctcgattat ttgaatatct
     2881 tttcctttat tcataactcg cgcgctacga gtaataacag gataatctct ataaattgta
     2941 tataataatt ctacttgaac atttgtaact gaatctaaaa tataaacttt caatgttttg
     3001 ctttcatcat tattatcaac ataggtagca ggtaaacctt ctagtttagg ttttccttct
     3061 tccacttcgt acccttgata gacaaaatct gataagcgag agccattttt ctgtagtaat
     3121 gaaatagcag gatgtctaaa atctgttgta ccatattcag gaaattcttg ttttaagtgc
     3181 tctaacgaat atagcaaatc gttttcattt ctataggttg tcataggccg atgatgcatc
     3241 tcaattagat gactatagtc attatctggt aagttctcac cataatataa atgcaataat
     3301 ttaccatctt ccgatatctt aaaaatgtaa cttatctcct gattaaacaa atgaaattgt
     3361 tggttttctg atcgataaat aatattcatt tcattatcct tatacataag ctctcctccg
     3421 tttctttatt gcattataaa aaacgctcac ataaatagta agcgtttaaa gtaacgaata
     3481 tttatcaaaa tcaaatgttt agtttattca ccattaatga ttcctagacc taatctacgt
     3541 tttcgatata ctcccggtga aacaccattt gctttcttaa aggctttaga aaaagattgt
     3601 actgttgaga atcctatctt gtcactgatt tgctggattg aatattcagt tgatgataac
     3661 agatcgtttg caatagaaat tttgatttca ctaatatact cttttattga tattcccata
     3721 tcattcttaa acaatcgtga taaataattt tcgtgtatcg atagactgac agcaatatct
     3781 ttaacagata atttttgata cgcattctca cgtattattt caattgtttg acgaacaaaa
     3841 ggattcatct ttttagtaat tatcccttcg ttcagataac tagctgaatt taagaggagt
     3901 tctaaaaatt tataaacttg tttttgtctg gacaattcac tgactagatt gtcctgatct
     3961 ttcagctgag tgttaggttc tgttactaaa tatatagaat tttccatatc caccctccta
     4021 tgttagattt ggataatttt tgttttatta tgtttatatt attac
//
LOCUS       DAYYGS010000144         3901 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000144 DAYYGS010000000
VERSION     DAYYGS010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.150"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(10..552)
                     /locus_tag="ACHA44_002711"
     CDS             complement(10..552)
                     /locus_tag="ACHA44_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HFM8905932.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            687..1076
                     /locus_tag="ACHA44_002712"
     CDS             687..1076
                     /locus_tag="ACHA44_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905933.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1080..1844
                     /locus_tag="ACHA44_002713"
     CDS             1080..1844
                     /locus_tag="ACHA44_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8905934.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIQLMEEASTFLAKNLIKKYK"
     gene            1973..2920
                     /locus_tag="ACHA44_002714"
     CDS             1973..2920
                     /locus_tag="ACHA44_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905935.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3050..3790
                     /locus_tag="ACHA44_002715"
     CDS             3050..3790
                     /locus_tag="ACHA44_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905936.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         1283 a          670 c          800 g         1148 t
ORIGIN      
        1 gtttgtgagt tattttcctt gtcgttttct cactttttta gtccagaaac cacctgaaat
       61 atattttggc tcataagaaa tgataaaagc acgctcttcg atttctttga tcaggcgata
      121 cagttcacgt tcgctttttc taggagaaag tatttcgaga actagccgct caccttccag
      181 ccccattgca gaagtctgtg tcacaccata accgtgttct ctcagtatac ccggcaaact
      241 tttatcttct gcgctatttg ctggcaaaat cgttgtgacc atgatgtatc ctaatgccaa
      301 tttgtcttca atcaaaattc cgacactgat acctactgcg taacctaacg catagacaaa
      361 taaattcagt ggattatcta aacgatctaa aacaagggat aagccgacga tataaatcgt
      421 gatctcgacc atactgacaa gtggagcaat cagtcgatag cctttcattg tcaacatgaa
      481 acgtaacgtg ttcagagtga tataagcaaa attaatgagg aagatcatcc ccaacatttt
      541 tggatcaatc atttgtaaaa ctcctttcat ttatataaca acaatatcaa aaaaaatcgt
      601 aagtgaagaa aaattgaaac tttatacata caaaaaaatg ataaaatgat tataatagaa
      661 ttataaagat aggttaggtg attaaaatga aaacggtcaa tcgtgtgttt tgtgccagcc
      721 acgtgctaag tctccttctc agtatatact taggtgtcta ttcgattcaa cagccaattg
      781 aggtagagaa cattgttttt cagatgtcgc tgacagatgg tttaatttta agcgtttttt
      841 tctttttgat actgtttatt ggtaatattt tcggtagcgt tgccagtttt ttgaattttt
      901 ctatttatcc actcctttcg ctagcattag gagtggtggg actttgttct ctgtgcttat
      961 ttcccgagcc attcagtcct gcatttatat tatttggtac actgaacctg ttccagacaa
     1021 ctgttggagc atggttgcta tggcgatcag gaaatttaat gaaaataggt gagtaacaat
     1081 tgaatccaat tatattgaaa cgttctgtcc atcatattcc agtgttagaa gttgttctga
     1141 aagaattaag agaggaacgt attcccgttg tcatctatta tcacggatgg cagacaagta
     1201 aggagttagt tttgacgcaa ggaagaaaaa ttgcgcagca gggcattcgt gtgatcctgc
     1261 cagatgcgat gaatcatggg gagcgcaaac agccggtttc ttctattcca tcctatacat
     1321 tttgggatag tatctacgga aatctgtttg aatttgacac acttatcaag catttagaaa
     1381 aaagaggtct tctacatgat cagcttgcag ttggcggggt ttcgatgggg ggaatgacaa
     1441 catgcgcctt gttggctaaa catcctcata tagtagcggg cgtgtgtttg atgggttccc
     1501 ctgcaccttt ggaatatgga aaagaaatcg taaggcgcgc atcggaatat aattatgtcg
     1561 taccaaaaga ctattttgat ttgatttcat gggttaagag ctatgatttg tcattgcagc
     1621 cggaaaagtt agctggacgt cctctgttct tttggcatgg acaagctgac gaaaaaatcc
     1681 cttatataca aacggaacga tttgtagaaa aaaatcggca taagtcttac ggaaaagaaa
     1741 ttgtttttcg ttcctcaaaa acacagcgac atatggtaca aatacaacta atggaagaag
     1801 cttccacttt tttagcaaaa aatttgataa aaaagtataa ataattgaaa cggaaaatca
     1861 ctcatgacga tagaggattt tccgtttttt attttgggct ataataaact ctgttaggaa
     1921 aatttggtat aattgatagt aagaatgaca acatgttaga aaggaagaga gcatgcccac
     1981 tttagcagat gttgcaaaac gtgccaatgt ttcaaaaatg acggtctctc gagtcatcaa
     2041 tcatccggaa caggtgactg acgaattgaa agagttagtt ttctcagcaa tggctgaact
     2101 ggattaccgt ccaaatattg ctgcaaaagc acttgtcacg aatcggtcgc agatcatcaa
     2161 attatttata ctggaagaaa tcgacacaac tgagccctat tacatgaatt tgctgatggg
     2221 aatcgctaaa cggatcggta aagcgcatta ttctctccaa ttagttacaa atgatgcctt
     2281 cgacgtcggt tcttgtgatg gatacatcat cacaggtgtc agaaaaaaag acttcgattg
     2341 gatcgaccgt ttggaaaaac cagttgtctt gtttggggaa aattctgaag gatatgatta
     2401 tgtagacagc gataatgaat atggtacagc aaaagcaacg caatatgcct tagaaagaga
     2461 ttatcaaacg attatttata tcggtatcga agtggacgag ccttttgaaa tttcaaggga
     2521 aactggttat ttatctgtga tgaaggaagt tgatcttcct agtaaaatct tgcggtttcc
     2581 aaatcattcg acagaagtag aaagatatat gacagaaaac tgggcagatt ttacagataa
     2641 aacttgtttt gtatgtagtt cagatcggct agcactagga gcagtaagag ggattataaa
     2701 tgctggagga cacttgcccg aagaatttgg tgtgattgga tttgatgggg tatttttaga
     2761 ccaaatcagt tcacctaaat tgacaacgat caagcaagat attatacgta tgggtgaagt
     2821 atgtggagaa atgttgttaa aaaaaataga agaaaaaggg gcgagtcaag gttatcgtcg
     2881 tttttttcct gaactagtta tcagagagac aacgaattaa gtcttattac tatatatata
     2941 tttatgaaat tcatcgctat tttcttaaaa taatcagcgc attgctactg aaaacaatga
     3001 atctgtgcta ttattgagaa aagttccatt tttgagaagg atggtttgaa tgaaagaaat
     3061 cagacgaaaa catttccgtt ttcctgcgta cgatgatgag ttaggcgtaa aattaaccaa
     3121 cgacaagaag cgagtattat ttgacggaca agaagatctt ttgactgaac agaatcagtc
     3181 cccgtttgaa cagatgaaag aatttcattt cacgaacacg aacaccgaga aaatccgtcg
     3241 caagcgtcgt gcaacaaatg gattgaagtc agctccgcgt aaggaaaatt tggaaaagca
     3301 taaaggaaaa ttaccggatt ataccggcca ttaccaatca gaaatcacat cgacaggacg
     3361 aaaaaaactt tttggtgatt cctctcgttc tgtcagacaa agtgaaacaa ggacagagtc
     3421 caagcatact acacgaaacg catctgttga cacgaaggtg cgggagcgtt cttattttgt
     3481 tcctaaatat gtacccgcat cgattatccc agaccctaga gaaagcaaaa tttctgagga
     3541 tcatttaatc gaatcgatga aaaaaacgga tgactcttat ctgttgttcg atacagaacc
     3601 gacgccgtat caagaaaaaa gaaatggtga gccgtctgta cgaacattca atcgaacaaa
     3661 aacgttagag gaaacaaagc agtcagaaga aaaaaaatcg aaaaaaagcc atggtatctt
     3721 agaacgctca ttacaaggat tgatcgatga tcaaggaaac tctttgggag aaaatagcta
     3781 ttttaaataa gtgatataca aatttatatt cggctacagt tggaaaggaa acgctgcggc
     3841 gtttccttat tttatactct atccgaaagt ttatatcgta tcctcaaatt tttttgagtt
     3901 t
//
LOCUS       DAYYGS010000145         3877 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000145 DAYYGS010000000
VERSION     DAYYGS010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.151"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(717..3866)
                     /locus_tag="ACHA44_002716"
     CDS             complement(717..3866)
                     /locus_tag="ACHA44_002716"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8905937.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMEPVKKESYLDPTVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYELLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
BASE COUNT         1077 a          803 c          635 g         1362 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacaat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttggacac ttgaatctcg gctgttttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcttccttca cgaatgggct gattaggaaa ctcttttgtt
     2041 aaccagtcag gttctttcgt catttctttg atcgctcgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat cgcagacttc gttggacggc catttcgaaa atcaaatttt
     2161 gtataggcat tgtctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag tagggtccaa ataactttct tttttgacag gctccatttg atcaatcata
     2281 cgattgatct gttccggtga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctaatgaagt cggctccacg gtgttttcat gctctacttg aaatcctaaa
     2401 accgagccat cttctaatgc attcttgatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggtcg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg gaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg ccccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgttttt ttgcatattt caacgccgca
     2701 tctagctttt gacgggttgt aataatcaca acatttgcat tggcatctgc caacagagtt
     2761 tcacttaact ctttggcact tcctgttccc acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aatttcgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gtccgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtt tttcctgaac cagtcgcatg ccacacatat
     3001 cctgattgat gtttatttgc agcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgaggatttt attgtcttga tcttcactga caatcgtata atccgcaatc
     3121 aagcgatgag catccgggat atttagcact tgtttgcaaa actcatataa gttttctacc
     3181 tttttattat ctttcgttcg ccaactaaat aaaaacttag gatgcaaatc ctttggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaacgcctga
     3361 taaaaactat cttttgcggt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 atcagtaagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata ggtagagatt cctccaccaa tgtcttgatt agaataaaga
     3541 atgagcgaga cagaacccag ttgcggatct tcccgttcaa tcgtgatccg agccattcca
     3601 ttttcacctt ttaaccattt cgcagcatca aagggggttt tcgttcgtag taacaattct
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagatga ttttttggcg taaattgacc caaagatctt cttctgattt tagatcggga
     3781 cgatacgtcc actgattatg cccttccccc aatacacgga tcaactgttc ttcgacttcc
     3841 gcttcctcac ggtgactaat tttcatttat cttccct
//
LOCUS       DAYYGS010000146         3871 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000146 DAYYGS010000000
VERSION     DAYYGS010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.152"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHA44_002717"
     CDS             complement(163..2160)
                     /gene="tkt"
                     /locus_tag="ACHA44_002717"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFM8905938.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2262..2513)
                     /locus_tag="ACHA44_002718"
     CDS             complement(2262..2513)
                     /locus_tag="ACHA44_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HFM8905939.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2660..3283
                     /gene="lexA"
                     /locus_tag="ACHA44_002719"
     CDS             2660..3283
                     /gene="lexA"
                     /locus_tag="ACHA44_002719"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFM8905940.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3463..3681
                     /locus_tag="ACHA44_002720"
     CDS             3463..3681
                     /locus_tag="ACHA44_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905941.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1073 a          820 c          716 g         1262 t
ORIGIN      
        1 tctcaagaaa cacaaactaa tccgaagaat tctttgtttt gtcatttaaa tgtaacatag
       61 ccgaaaacga aaagtaatat tgatttattc ttctaacaca taaagaacag ctgtctccaa
      121 cttgcagaca gctgttctta tcagtcaaac aattgcttgg atctatgata gttgattgta
      181 cgtattcacg acattttcta ctgtaaatcc aaattcttct aaaattttag tgccaggggc
      241 agatgcaccg aaatgatcga ttgtaatcgt tgcgccctct gttcctacgt agcgttccca
      301 tccaaatgat gcagcagctt cgatagccac acgtttcttc actgatttag gaagtacact
      361 ttctttatat tcagaagatt gtttttcaaa gaggtcaaag cttggcatag aaacaacaga
      421 aacatctttg cctttttcag ccaattcttt ttgagctttc acagccaaat caacttcaga
      481 acctgttgca atcaaaatac cttctggtgt ttctccttgt gatggagaaa gaacatacgc
      541 gccttttttc accatttcat ctgctacttc ttttgtagaa ggcaatactg gaaggttttg
      601 acgactcaat acaaggatag taggcgcatc tgttgtctcc atcgctactt tccaagcagc
      661 acgtgtttca tttccgtctg ccggacgaat cacttgaaca ccaggcatgc agcgaacaga
      721 tgctagttgt tcaatcggtt cgtgcgttgg tccgtcttct ccgacagcaa ccgagtcatg
      781 tgttaagacg taaacaacag gagtatgttg gattgctgct aaacgaactg ctggtcttag
      841 ataatcgaca aagacaaaga atgttccacc gtagatacga gttccgccat gaagttggat
      901 accattcata gccgaagcca ttgcaaattc gcggacaccg aaccagatgt tgcgtccttc
      961 ataatgttca ggagtgaaat ctttatctgc agccaccatt gtattattgg aacctgacaa
     1021 gtccgctgaa ccaccccaga agcttggtat agctttcgat aattcttgga tgacttcttt
     1081 acttgaaaca cggcttgctt gactgcttcc aacttcataa gttggcaatt ctgcatccca
     1141 gttttctggt agtttgccat caaaagcatc ttcaaattgt tgtgctaatt caggataagc
     1201 ttttttataa ttggcaaaca tttcattcca tgcatcttca gctttttgtc cttcttctat
     1261 catcgtttga tggaagcggg cagctacttc ttctggaaca gtgaaatctg gatactccca
     1321 accataaact tctttagcca tcttgatgcc atcttcacca agaggagcac catgaacagc
     1381 agatgttcct tctttaggag caccatagcc gatgactgtt tttacttcga tcaatgttgg
     1441 tttgtctgtt tcagcttttg cttcttcaat cgctttagag attgcttcta ggtcatttcc
     1501 gtctttcact aagatatgtt gccagccata tgcttcatag cgagcaccaa cgttttctgt
     1561 gaaagctttt gaagttggac cgtctagtga aatatcgttt gaatcgtata aaacgatcaa
     1621 tttccctagt ttcatatgac ctgccatcga acttgcttct tgagataccc cttccatcaa
     1681 gtctccgtca ccgcatattg catacgtata gtgatccatc acattaaagt tttctttgtt
     1741 atacgttgct gcaaggtgcg cttctgccat tgccataccg acagccattg caatcccttg
     1801 tcctaatggc cctgtagtgg cttctacacc atctgtatga tgtacttcag gatgtcctgg
     1861 tgttttagaa tcccattgac ggaattgttt caaatcatcg attgtcactt gatagccagc
     1921 taaatgaagc aagctataca acattgctga cccatgtcct gccgaaagaa cgaaacggtc
     1981 gcggtcagcc cagttttttg atgtcgttgg atttactttc aggtgttttg tccataacgc
     2041 atatgccata ggtgctgcgc ccatcggcaa tccaggatgt cctgagtttg ctttttggac
     2101 tgcttcaata cttaatgtac gaattgtgtt aacgccaagt tggtcaattt tgtcaaacaa
     2161 aataacccct cctattaatt taggtttttt ttacctttat ttcttacact ttcattctat
     2221 aagatgtcac acgaaaaagc aatctttttc agtatttttt actaaaagtt gtcatttcgg
     2281 ttgtgtaagc cttttttctt ttggatttct tttaattttt ccggggtcac gtcattgccc
     2341 tcaggatcga cgactttcat tccttcgata tgatgacgca taccggaacg aaaagcttcc
     2401 aaatactctt tgcgcaagtt gatttgttct tgttcttcgg cttttgagag accattttcc
     2461 ttctttttac gtgcaagttc attgatccgc tctatttttt caggtgacag catgctcagc
     2521 cctcctttta ctctccttca tattatataa aaactgaaaa aaaaacaact agataaacta
     2581 aataaaatac gaacattcgt ttgatttagc gctttttata tggtactatt tatttatcaa
     2641 gtgaaagaag gtgtaccttg tggcccgaca aactgaatca agacaaatcg aagtcttacg
     2701 atatatccat gaacaagtaa cccaaaaagg atacccgccg actgtccgtg aaatcgggga
     2761 agctgttcag ctttcttcta cttctactgt tcacggtcat cttgcacggc ttgaaaaaaa
     2821 agggtttatc caaagagatc cgacgaaacc tcgtgcaatc gagttgacaa aacaaggttt
     2881 agaaaagatt gggattcgtc caacaactat tccagtactt ggcgtcgtaa cagctggaga
     2941 acccatttta gcagtagagg atgcttcaga cttctttcca atccctccta atctttcttc
     3001 cgaagaaggc agtctcttta tgctgacaat tcgtggagat agtatgatca atgctgggat
     3061 attggatggt gacaacgtga tcgttcggaa gcaagaaact gctcaaaatg gcgatattgt
     3121 cattgcaatg actgccgagg acgaagcgac ttgcaaacga ttctataaag aaaaaaactt
     3181 tttccgtctg cagcctgaaa acgacttgct cgaaccaatc atcttagatc aagtatctat
     3241 attaggacga gtagtaggac tttatcgtag tcatatctat taattgctac taattaaaag
     3301 aaaacttttt aaaaagagtt tgagacgttg gtctcaaact cttttttcgc ccgggttctt
     3361 cttctttcaa atttttatca cttccctctt tacaaaccga acaaatattc gtatactaaa
     3421 tatacgaaca attgttcgca taaattaaag aggtgagaat tcatgaaatc aatgagacgt
     3481 cttggcttag tattaactgt tttgttttta ggtattatta ttggtaattt cggcatacgt
     3541 ccagctttac ttgtgtttgt tccgttattt actttgtggt tcatgttgtg ggatgaaaag
     3601 aagtaccgtc aatctgagcg gcgcagaatt catgaagatc atttctatca tgagccctac
     3661 tacagatcca ggcaatcata ataaggattg actgctaatt attcaaaaga caagaagtta
     3721 aacttttacg ggggtgtgac aaaaatcggt cacatccttt ttttcgaata aacggtgttc
     3781 taaagccagt ttttcggtat taagtcaaat tgcggaaaaa gtgatttttt aaacttctac
     3841 tggtctttgc ttgtttccat ttataaaaaa a
//
LOCUS       DAYYGS010000147         3776 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000147 DAYYGS010000000
VERSION     DAYYGS010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.154"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            127..579
                     /locus_tag="ACHA44_002721"
     CDS             127..579
                     /locus_tag="ACHA44_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905942.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="ACHA44_002722"
     CDS             593..1282
                     /locus_tag="ACHA44_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905943.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="ACHA44_002723"
     CDS             1407..3758
                     /locus_tag="ACHA44_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HFM8905944.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
BASE COUNT         1282 a          494 c          777 g         1223 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaa
//
LOCUS       DAYYGS010000148         3744 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000148 DAYYGS010000000
VERSION     DAYYGS010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.155"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..485)
                     /locus_tag="ACHA44_002724"
     CDS             complement(<1..485)
                     /locus_tag="ACHA44_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905945.1"
                     /translation="MWSLVGIVLIIFTVTAATEANYSHKIQSYKGRLVMTKKDVTRYT
                     SSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWENASHKAYNSKIVYTVEDADEQNIT
                     LPVFNYWGTVVKVNGKKSTIENDNGLVSIKGTGKRTIVEVSSTYPTTMILSLLISSIT
                     F"
     gene            complement(625..1305)
                     /locus_tag="ACHA44_002725"
                     /pseudo
     CDS             complement(625..1305)
                     /locus_tag="ACHA44_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
     gene            complement(1627..1848)
                     /locus_tag="ACHA44_002726"
     CDS             complement(1627..1848)
                     /locus_tag="ACHA44_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905946.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1946..2191)
                     /locus_tag="ACHA44_002727"
     CDS             complement(1946..2191)
                     /locus_tag="ACHA44_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905947.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            2431..3045
                     /locus_tag="ACHA44_002728"
                     /pseudo
     CDS             2431..3045
                     /locus_tag="ACHA44_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(3415..>3744)
                     /locus_tag="ACHA44_002729"
     CDS             complement(3415..>3744)
                     /locus_tag="ACHA44_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8905948.1"
                     /translation="MDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKS
                     GILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNL
                     DESDLPF"
BASE COUNT         1199 a          686 c          545 g         1314 t
ORIGIN      
        1 ataaaagtta ttgaactaat taataaagat agaatcatcg tagttggata cgtagatgaa
       61 acctcaacta tagttctttt ccccgtacct ttaatagaca ctaaaccatt gtcattttct
      121 atagtgctct ttttcccatt tactttcaca actgttcccc aataattgaa tacaggtaat
      181 gtgatatttt gctcatcggc atcttctact gtatagacta ttttggaatt atatgcttta
      241 tgagatgcat tctcccaccg atcaccaact tttacttcat gtttctttaa atgatcttta
      301 tatttcaatg catctaatgg tgcatagtca tataggcctc cagttcttga ggatgtataa
      361 cgtgtaacat cttttttggt cataactaaa cgacctttat acgactgaat tttatgcgaa
      421 taatttgctt ctgttgctgc agtcacagta aaaataataa gaactattcc tacaaggctc
      481 cacatacttt ttttgttgct tgttttccac tttaaaaaca ctatggttaa gattaatgaa
      541 ccaaaaagca cttgaaaacc caaggttctc ggttctgttg cgaagtttga aaatcaaacg
      601 cgccctctct gaactccaaa tatcttaggc tgttattccc attaatacct tgatttcagt
      661 agacaccgaa aagccgaaga gcgttccatt tcttcggttc tttttatata ttcctcgaat
      721 ggtctccatg cccttaatcg tggaagaggc tgtacggaga ctttgataaa atttatttcg
      781 tcgtttaata ggtcgatggt cttgttctat taaattgtta agatacttca cagttcggtg
      841 ctctgtctta gtatataaac ccacactctg taactttcta aaggcggagc caagagaagg
      901 tgctttatcg gtcacaattg ctttcggctc accaaactgt ttatggagtc gttttaagaa
      961 agcataggct gcttgcgtat cccgtttctt tcgtaaccag atatctaagg ttaagccgtc
     1021 cgcatcaatt gcacgataaa gataatgcca acgtccctta attttgatat aggtttcgtc
     1081 cattttccat gaatagaagg attgtctatt tttcttcttc caaagataat agaggacttt
     1141 gctgtactct tatacccaac gataaatcgt agtatgacaa acatttattc cacgatcata
     1201 taacaattcc tgaacttcac gatagcttag attgtaacgc aggtagtaac caacagcgac
     1261 aataatgacg tcttttttga attgtttgcc tttaaaatga ttcattactc tgtcctctct
     1321 gtcttttttc tcaattttac actaaaatag attttttgga aaactttgca acagaaccgt
     1381 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
     1441 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
     1501 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
     1561 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
     1621 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
     1681 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
     1741 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
     1801 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
     1861 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
     1921 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
     1981 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
     2041 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
     2101 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
     2161 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
     2221 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
     2281 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
     2341 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     2401 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     2461 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     2521 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     2581 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     2641 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     2701 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     2761 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     2821 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     2881 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     2941 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     3001 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     3061 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     3121 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     3181 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     3241 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     3301 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     3361 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     3421 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     3481 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     3541 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     3601 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     3661 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     3721 tgaaaagata gaattttcat ccat
//
LOCUS       DAYYGS010000149         3716 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000149 DAYYGS010000000
VERSION     DAYYGS010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.156"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            72..155
                     /locus_tag="ACHA44_002730"
     CDS             72..155
                     /locus_tag="ACHA44_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8905949.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHA44_002731"
     CDS             280..1017
                     /gene="erm(B)"
                     /locus_tag="ACHA44_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8905950.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1280..2143)
                     /locus_tag="ACHA44_002732"
     CDS             complement(1280..2143)
                     /locus_tag="ACHA44_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8905951.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2145..2417)
                     /locus_tag="ACHA44_002733"
     CDS             complement(2145..2417)
                     /locus_tag="ACHA44_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8905952.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2434..2643)
                     /locus_tag="ACHA44_002734"
     CDS             complement(2434..2643)
                     /locus_tag="ACHA44_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8905953.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2721..3617)
                     /locus_tag="ACHA44_002735"
     CDS             complement(2721..3617)
                     /locus_tag="ACHA44_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8905954.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1143 a          652 c          575 g         1346 t
ORIGIN      
        1 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
       61 agggattcgt catgttggta ttccaaatgc gtaatgtaga taaaacatct accgttttga
      121 aacagactaa aaacagtgat tacgcagata aataaatacg ttagattaat tcctaccagt
      181 gactaatctt atgacttttt aaacagataa ctaaaattac aaacaaatcg tttaacttct
      241 gtatttattt atagatgtaa tcacttcagg agtgattaca tgaacaaaaa tataaaatat
      301 tctcaaaact ttttaacgag tgaaaaagta ctcaaccaaa taataaaaca attgaattta
      361 aaagaaaccg ataccgttta cgaaattgga acaggtaaag ggcatttaac gacgaaactg
      421 gctaaaataa gtaaacaggt aacgtctatt gaattagaca gtcatctatt caacttatcg
      481 tcagaaaaat taaaactgaa cactcgtgtc actttaattc accaagatat tctacagttt
      541 caattcccta acaaacagag gtataaaatt gttgggaata ttccttacca tttaagcaca
      601 caaattatta aaaaagtggt ttttgaaagc catgcgtctg acatctatct gattgttgaa
      661 gaaggattct acaagcgtac cttggatatt caccgaacac tagggttgct cttgcacact
      721 caagtctcga ttcagcaatt gcttaagctg ccagcggaat gctttcatcc taaaccaaaa
      781 gtaaacagtg tcttaataaa acttacccgc cataccacag atgttccaga taaatattgg
      841 aagctatata cgtactttgt ttcaaaatgg gtcaatcgag aatatcgtca actgtttact
      901 aaaaatcagt ttcatcaagc aatgaaacac gccaaagtaa acaatttaag taccgttact
      961 tatgagcaag tattgtctat ttttaatagt tatctattat ttaacgggag gaaataattc
     1021 tatgagtcgc ttttgtaaat ttggaaagtt acacgttact aaagggaatg tagataaatt
     1081 attaggtata gggcacctct aataactacc aattaattca cctcaaaatg aaattagaaa
     1141 aaaacagcga aatttttaaa tctatttctt atcgatacaa attcctcgta ggcgctcggg
     1201 accccaacat gaggaaatgt ttttataaaa aaagagtagt taccgttttt tggtaactac
     1261 tcttttcctt ttttaaggtt taaatacctg gaagtttagg tgttttgggt attggtggtg
     1321 taggtagctg caagctttcc attttttgtt gaattgcttt aaattcaggg gtttcttggt
     1381 gttgattttg aaccattttt tgctctattc tctccaaagt cggttgaatt tctttccctg
     1441 atactttacg atttaattct ctttctaagg tctctttcgg actaatggaa ggcgtttcta
     1501 agcttgaata gagtttcact ccttctctat tgtaaagcct tatatcgcta aataagcccg
     1561 ttttatgaag ggtttctaaa ttggtcggta agtttttgac aacaatatca tgcgcttgtt
     1621 ttggtgttgc cctagctgtc attggatcat ctgcatacat ggtttcatat cgttcaatcg
     1681 ttcctaagta tgattcaatt ttcggtactg ccatgacgta tatttttgtt tcataacctt
     1741 tagattgaag cattgtggct gtttgaatgg gaacgtctgt tgttcgtcct gttccttcaa
     1801 tcactaaatt atagccttga tcgcttaaac ggctaataag tgcttctgtc atgcgattcg
     1861 aataaggggt aacgtgtttt actacgtctt tttcatagag tttcactaat tcgtcaaaat
     1921 ttgggtgctg ttgtttaaag gtatcattat caatgacaat aacattccct tgtgtttctt
     1981 caaaaattgc tgatcgcaaa ctggttttcc ctgaccctgg ttgcccacca agtaaaaaag
     2041 cggttggcga ttcaaccgct agtctatttt tagttagtcg ttcaacattc ttttctaaac
     2101 gatcttcaaa ttgcttttcg gtgaagtcag taatattcgc catgttaagc cactttctct
     2161 ttattcaaaa tttgttttga atattgattc attgaccgcc aatactcatg aatagcttgt
     2221 aattcttcaa atggttgttg aaataaatca acttcttgtg ccacttctaa ttgatttaaa
     2281 agattcactt ctagtaaacg agtattttta gtatctagtt catgattcaa aacataattt
     2341 aatacccgat tataaacact tgccgataac tcattaatga tttcaatttc aaatgttttt
     2401 tcatacgtaa ctgccatttc attttcactc cttttaaagt ttgtcaggta aatatttccg
     2461 tatatattct tctaaggctt gatccataac ttcttttacg tttccgccat tctttgctgt
     2521 ttcgattttt atgatatggt gcaagtcggc acgaacacga accgtcttat cccccattat
     2581 atcttttttt gcgctgattg gcgtatcatt tcgttttgct ttctgtgcgc ctaaatttcc
     2641 cacgatcgct cacttctttc tatttcttct tactatttat tttatcatca cctatcacaa
     2701 atcacaagtg atttgtgatt ctattctttt tcattttcta attgaatgat tcgctcaatc
     2761 atttcaaaaa atacgttctc atacatagac aaaacttttt tgtcatagcc tttgtgttct
     2821 gtaatgccat ttttagacca agtacttact ttattacttc gcttgataat atttcggaaa
     2881 accaaattat cctctttatg ttctttgtac agttcttcca ggtttgattt tatcgttgcg
     2941 ctgtccgtat caactaaata aggaacaaaa ccaatcatat ctagtccagg gttaaactgt
     3001 tcttgcaaat caatcaaata ggaaatatag ttttgaatgt tgtttgtact ttcttcttct
     3061 gcttgtaaag ggatcataac gtaatcactc gccacgattg cattatttgt ataaacgctt
     3121 ggcgttggta cagtatcaat aataataaga tcatagtcac tttttaaagg tgctaaaaga
     3181 gtagcaagca atctactttc attttcaaac gtccatgagc gagttaattt tggcagtaac
     3241 atcaaatcaa acgtgccagg gatcaagtct aaattatcag tcaaatgaat aatagaagaa
     3301 gccaagtttc catttttcaa gccttcataa aaattgacac gtggcaattc tacctcaaaa
     3361 gtttttgcta agtcttttgt caatgttgcc tgtaagtcct tatcgatcat taaaactttt
     3421 aaattcaatt tgtctgtcaa gtaagcaaac atagtcgata atttggactt tccaacacca
     3481 cctttaaagt aattattcaa gataataatt gcttcatttt tattgtttaa aatacgtctt
     3541 aattcttcaa gtatttttag tttttccttc tccataacta cgccccattc tttttttgta
     3601 tagtaatatt gtatcattaa gaatcacaaa tcacaagtga tttgtgatta atcacttgtg
     3661 atttgtgata agtgatttgt gattaatata taaaagccct ctttaaaggg ctttta
//
LOCUS       DAYYGS010000150         3669 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000150 DAYYGS010000000
VERSION     DAYYGS010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.157"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(42..1121)
                     /gene="uxuA"
                     /locus_tag="ACHA44_002736"
     CDS             complement(42..1121)
                     /gene="uxuA"
                     /locus_tag="ACHA44_002736"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFM8905955.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            complement(1124..2755)
                     /locus_tag="ACHA44_002737"
     CDS             complement(1124..2755)
                     /locus_tag="ACHA44_002737"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFM8905956.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            3065..>3669
                     /locus_tag="ACHA44_002738"
     CDS             3065..>3669
                     /locus_tag="ACHA44_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFM8905957.1"
                     /translation="MKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRTPI
                     REALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQKK
                     RILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERFRW
                     LRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLH"
BASE COUNT         1193 a          719 c          515 g         1242 t
ORIGIN      
        1 agaagagaaa atttttcctc taaataaact cccttatata attatttact cgcccccttt
       61 tcaagcgctt cccaaatacc atttaaataa actgcaccta gagctcggtc atataatcca
      121 taaccaggtc taccggtttc tccccaaatc atccgaccat gatctggacg aatatagcca
      181 ttaaatccat tatcgtacat tgtttttaat atcttataca tatctagaga accatattct
      241 gatttatgcg gcgcttcgta aaaatcgagt ccattgtcac ttaaatattt gatattacga
      301 acatgtgcaa aaggaatccg atcccgttga caaaattcag atagaatttc atagacatca
      361 ttatctggtc gttctgcaat acttccagta cataatgtaa taccattaga aggagaatcc
      421 actacattac acaaccaatc taaatcgtca cgatttttaa caatacgagg taatccgaaa
      481 atggaatatg gtggatcatc tgggtgaact gccattttaa tcccaacttc ttcacatgtc
      541 gggataattg ctttgagaaa atatgcaaag ttctctcgta atttctcttc atcaacttct
      601 ttataggctt ctaataattg cttaatttgt gataaccgat gaggttccca acccggcaat
      661 tcaaaattat tagtagaatt ttctacttct ctaataattt cctccggatc agtagggata
      721 ttttttgaaa tataggctaa cgtagaagac ccatcatcta aacgataatc caaatccgat
      781 tttacccaat caaatactgg cataaaatta taacagataa cctcaatccc aaattctgcc
      841 aaattgcgaa tcgtctgttt ataattctca atgtattgat ctcgtgttgg taaaccaatt
      901 ttgatatcat catgaacatt aacactctca ataactttca aggtaagtcc attggcttcg
      961 acttcatctt ttaaagattt aattcgttct ctcggccaaa cctctccggc tggtatatcc
     1021 attaatgttc caacaattcc tttcattgct gggatctgac gaatttcttg tagtgagatt
     1081 ttttcttcat ttgccccata ccatctaaaa gtcatttcca tagttacttt cctccaactt
     1141 ctgttatata tttattgatt gttttacgaa ctgcttgagg acctactagc atttcgcaaa
     1201 agattttttc agttttcttt cctaccccaa cttgatataa atctaaacca aagatttctt
     1261 tattttctaa aatttcctgt accgcacaat gaatctttga aggatcttgt tcacctaatt
     1321 tcaatactga taatttatcc tgcaactcta ccaataacgg atcaggactt ggcacaaaat
     1381 tttccccttg atcatcgact gccagtaaat aacgtaacca agcagcaata gcgataggaa
     1441 taaatgttag attttctgca tttccgtctt tttcgacata ctttttaatt gtttcaccat
     1501 agcgaatcgc tactttttga gaagtatctg ttgcaattcg ttgtggtgta tctggaatat
     1561 agggattagg caaacgttta ttaattactt catcaataaa ttgttttgga tcaataatgc
     1621 ctggattttc aactactggt aaaccttcta aataacctat tcctttgatt aaaccaacca
     1681 aatcatcatc ctgcatctct gctgaaattg attgatatcc tagcagacaa cctgttactg
     1741 ccaaagcagt atgaagtgga ttcaagcttg tcgtaacctt catgatgtct gctttatcaa
     1801 ccgtctctct atctgtcata atcacaccag ccttttccaa tttaggccgg ccatttggaa
     1861 aactatcttc aataactaaa taatgaacta cctcagtatt agtgaaagca gcaatattgg
     1921 tatgtttttt tgtgtgaatg atatctaaat cctcgagccc catcatcaat agatcttcct
     1981 ttacattctc tgaaggattt ggcgtaattc gatcaatcat actccaagga aaagtcactt
     2041 tttctttatc ctttaaataa gcaataaatt tttcagaaac atatccttct ttttgccaag
     2101 cttctgcaat cgtaaaaata ctatcttgaa aacgttgacc attctgtgag aaattatcag
     2161 tagaaaccat cgcaatcggc aactctccag cattaaatct gctatataac aaggatgtta
     2221 caatactcat cgtatgttga ggattgctag gtccagattt gatatcagct ttgactaccg
     2281 aaaagaattg ccctttagaa tcttttaaac tgtaaccttt ttcagtaata gtaaacgttg
     2341 taaactgtaa tttaggatct tcaaaatatt taaccactct ttgatagtcc gctatattct
     2401 tcggattgca atataaacta tcagccgtta ctgccaaaag tctttttttg agttcaccat
     2461 ctccactcat aacaatttcc aaaatgtcat tgttatagtc atgataagct ttttccacta
     2521 cttcttcatc aaatgtttca cacacaacta ctcctgtttc taattcattt tgatctgcta
     2581 agcattgagc aatttctgca tgaaatcctc gataaagatt tcctccacca aaatgaagcc
     2641 atactggatg ttgctggcct tcttgcttaa cttctttgac atcaaaacac ggcagtagaa
     2701 tctgcatttc attgaattct tcgatagaat tttgccaatt tttaatcgaa accataatta
     2761 acctcctcaa actgatatgt tagttttgat acattttata ttttaaaacc cattgtaacc
     2821 gttgtcaata taaaaaggga ataaacttgc ttttttctac catatcagaa tgattgtatt
     2881 ttttattcag aaaaataaaa tactccttct ttttggactg attttatcga aacttatttt
     2941 ttcttttttt catgtattct tttattttta taactagtgt attacttcaa aatttgatat
     3001 tatatggcat gaacagaatt aaaatatcag ttaaaataaa agttaaggag cattgtgtat
     3061 gacaattaaa actagtctcc aaaatcaagc ttaccaatct attcgccgac aaattatcta
     3121 tgctgactta gaacctggaa aaaaagtatc agaaaaaaaa ttagaagaaa cactttctat
     3181 tggtcgtaca ccaatacgtg aagcactgat tcaactccga caacaaggtc ttgttgatac
     3241 aattcctcaa agcggaaatt atatctcatt aattgattta gattcggcca aaaatgctcg
     3301 atttgtcaga gaacatttgg aacgacaaat tatgttagag tgttgtgcca aaatgaataa
     3361 ccaaaaaaaa cggattttag aaactataat ttcagaacaa gaaaaagctg ttagtcaaaa
     3421 aaatgctcgt agtttttttc ataatgataa tttattccat aaagcttgtt ttgaaattgc
     3481 agagcgagaa gaagtatggc aatggttaga aaataacaat acacacttag aacgatttcg
     3541 atggctacga gtaaaaacgg aaggattaaa gtgggaaaaa attatggaac aacattatca
     3601 actatttgag gcattaacaa aacaagatcc agaagaagct aactttctaa catcgttgca
     3661 tcttcattt
//
LOCUS       DAYYGS010000151         3631 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000151 DAYYGS010000000
VERSION     DAYYGS010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.158"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1006
                     /locus_tag="ACHA44_002739"
     CDS             <1..1006
                     /locus_tag="ACHA44_002739"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8905958.1"
                     /translation="IILIRVRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQA
                     VLMAGQYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKA
                     REFGAMTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGS
                     KKWETCLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAA
                     CWFMEMQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSN
                     KLMVLDQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY
                     "
     gene            988..1449
                     /locus_tag="ACHA44_002740"
     CDS             988..1449
                     /locus_tag="ACHA44_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFM8905959.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1499..1963
                     /locus_tag="ACHA44_002741"
     CDS             1499..1963
                     /locus_tag="ACHA44_002741"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFM8905960.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            1977..2726
                     /locus_tag="ACHA44_002742"
     CDS             1977..2726
                     /locus_tag="ACHA44_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFM8905961.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2726..3556
                     /locus_tag="ACHA44_002743"
     CDS             2726..3556
                     /locus_tag="ACHA44_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFM8905962.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
BASE COUNT         1213 a          484 c          709 g         1225 t
ORIGIN      
        1 tataatactt attagagtga gggataaaga aatgagacaa ctacacaaag aacagattga
       61 aacagcaatg gcagcgttaa aggaaaagac aattgatcat gtatatttta cagcatgtgg
      121 gggttcacaa gccgttttaa tggctggtca atatctattt gataaagaat taactgtacc
      181 ttgtcatgtt tatactgcta acgaatttaa ctatgctata ccaaaaggat tatctgaaaa
      241 ttctttagtt atcacttgtt cccattctgg cacaacacca gaaactgtag aagcaacaaa
      301 aaaagcaaga gaatttggag caatgactat ttgtttatct aatgaagaag gatctcctct
      361 atgggaagca gctgaatatc ctgttcacta tgattggggt aaagatgttg atgctagtga
      421 tttaaataaa ggaattcttt atggtttatt atttaatatt ttacttgttt tagatggctc
      481 taaaaaatgg gaaacttgct tgaatgaatt tgataagtta aatgatcttg ttattgctga
      541 aaagagcaaa tttgaaataa ctgctaaaga atggggtaaa aaccaaaaac gagataaaat
      601 tatttacaca attggtagcg gtatcaatta cggagaaatt tactccacag cagcttgctg
      661 gttcatggaa atgcaatgga ttaattctaa tgctattcat tctggggaat ttttccatgg
      721 tccttttgaa atcactgatt atgatgtacc attcatctta gtgaaaagta ttggagatac
      781 aagattttta gatcaacggg tagaagactt cactaaaaaa ttcagtaata aattaatggt
      841 tttagaccaa gctgattttg acttagaatc tatagatagt gtagcgaaag aatatattgc
      901 agctgtactt agtggcgttg ttattcgcca aatggtagaa gcgattgctt ttgaaagagg
      961 ccattcctta gatgttagac gctatatgtg gcaaatgaaa tattaattaa taggaggttg
     1021 caaaagatgg caaatcaatt atttcacatt gtgattacaa cacattctac cctttgtgag
     1081 ggttatttaa aggcaacaaa attaatttta atgaatgacg ccacaggtat tagtgctgtt
     1141 ccatttaaag aaaatactag tccagatgat tttgaggaga gtatgaaaga aataattagt
     1201 cagcatcatt tgcaacctct aattatttta acggatttaa ttggaggaac acctaataat
     1261 gtttctatta aacagttgaa caacaataga attcaaatta ttgctgggat taatttacct
     1321 cttttactgg agttattaat ggcccagtca gctggtaaat ctatagaaga aatagatttt
     1381 aaggaattac tgaataactc tcaaaaaagt atggttcata ttaattcgct tttaaaagat
     1441 gtcagttaag gtggcaattt ctattctata aattttaaaa attgaaggag gaaatagaat
     1501 gattaaatta gtgcgtgtag accacagatt acttcatgga caagtaatat tttcttggac
     1561 taaacagtat gatattaacc atattattgt ggctgataac aaagttccag gagatccaat
     1621 gtctgtgatg gctttgacaa ttgctaaacc tgctgattgc aaattagaca ttgttaaaat
     1681 tagtgatgtt aaagggatta tggaaacatc cccaaatgat aattacatga ttttaattaa
     1741 aggacctaag gaagcttttg aattagttca aaatttacca gaaattaaag aaattaattt
     1801 tggcggtgtg tctaagaaaa gcgagtccaa acaatatgga aaggcagtct ttttaactcc
     1861 tgaagaatta gaagccacaa aaaaaattct tgatcaagaa gtgaaaatat ttgtgcaaca
     1921 agtcccatct tcaagtattg aaaatgtaga ttttagtaag taagggggga aaataaatgt
     1981 tattaaaagc cattttatta ggtttagttg gtgtggtagc tgttattgac tccaggttaa
     2041 ttggtcgaca aaatattggt agaccactaa ttttgagtac acttgccgga cttgtgctag
     2101 gtgacttgca tacagggatt gttcttgggg catctttaga attaatttct atgggattcg
     2161 tctcaattgg ggcggctgga cctcctaata tgcaacttgg tagtattatt gccactgctt
     2221 ttgcaatttt aactaatagt agtacagaaa cagccttaac aatagctatc ccagtggctg
     2281 tagctggtga atttatgagt attatcatgc gcatgataat tgctcaattt ggacatgctg
     2341 cagataaagc cattgaaaat aataaattta gaaaagctca aattattcat atttattggt
     2401 catttatttt taatgctttt gtttacttta ttccaatctt tttaacagta tattttggtg
     2461 ctgacgttgt ggcagattta gtagataaaa ttccagaaat gattacagac gccttgactg
     2521 tctctggaaa tatgctttcc gcattagggt ttgcaatgct attaagcaca atgttgagta
     2581 aaaaattata tccttacttt atttttggat tctttattgt tgcctattca ggtctttcac
     2641 taattggcgt gacaattttt gcagctctga ttgcctttgt aatggatcaa gttaaatatg
     2701 gaaaaagaga agaggcgcat ggttaatgga aaatagtaca gaaaaaaaac gtttaatcac
     2761 aaaaaaagat cttaataaga tgtgtcttcg ttcatggaca atcatgtgtt cgtggaatta
     2821 tgaaagacaa atgcatatgg gatttatgta tgggatggcg ccaattttaa ataaagttta
     2881 taaaaatgat gaagagaaaa aaagagaagc ttatactcgt catatggcat tttttaactg
     2941 tacgcctcaa atgacttcat ttattatggg actttgtgct tctatggaag aacaaaatgc
     3001 tcaagcagaa gaaggaaact ttaatactga atctatctcc atgattaaaa ccagtttaat
     3061 gggtcctttt gctggaattg gtgattcatt tttccaaggt actttaagaa ttattacatt
     3121 tgggattgga ttgtcttttg ctaagcaagg tagtatttta ggaccgattt tagctgttgg
     3181 cctttttgct ataccttcta ttttagctgt tttttacggc ggtaaattag gatatgagtc
     3241 tggcaataaa taccttacta aattgtatca agaaggtctt atggatcgag taatgcaatt
     3301 tgcttctatc attggtttgg ctgtagtagg tggaatggtg gctagcatgg tgccaattgt
     3361 caccccttta aaattcacta cagggggaac agaacttgtt ttacaagact tacttgattc
     3421 cattattcct ggattattac cacttgtatg tactttggga atatataaat tgattaagaa
     3481 aaatgttagt acaaatatgc tcttattagg ttgtattttg gcagggtttg ttttaagtat
     3541 aataggtatc ttataatgat taaggctcta atttttgata tggacggagt tttaattgat
     3601 agcgagatta tgtatcaaaa gtgggtattt a
//
LOCUS       DAYYGS010000152         3587 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000152 DAYYGS010000000
VERSION     DAYYGS010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.159"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(2..982)
                     /locus_tag="ACHA44_002744"
     CDS             complement(2..982)
                     /locus_tag="ACHA44_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFM8905963.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(1006..2490)
                     /gene="galT"
                     /locus_tag="ACHA44_002745"
     CDS             complement(1006..2490)
                     /gene="galT"
                     /locus_tag="ACHA44_002745"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HFM8905964.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(2502..3491)
                     /gene="galE"
                     /locus_tag="ACHA44_002746"
     CDS             complement(2502..3491)
                     /gene="galE"
                     /locus_tag="ACHA44_002746"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFM8905965.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT          941 a          779 c          652 g         1215 t
ORIGIN      
        1 tttatttcgt tgattctcga ataatcaact ctgtttccac aataatcttt cttgaaaccg
       61 gtgctttgga attacttaat tgaagaatcg tctccactgc tagctctccc atccattcgg
      121 tataaacttt tacagtagaa agagagggcg ttacatattt cgctacactg acatcattga
      181 aacccatcac agaaatatct tctggtacca ctaaaccttt ttcttgaatc gctttcagtg
      241 cgccaatcgc taatgcatca ttagctgcaa aaagcgcatc aggaacatgc ggattcgttt
      301 ttaaatactg cttcactgct tgatatcctg actcgacaga aaaatctgct tcgataattg
      361 gaccttcgtc gtagcctttt tctgccaaaa cttgttgaaa tgccataatt ctgggatctt
      421 ttagccgctg atggtttttc ttcgtatatt ctgttccagc aagcatagcg atatgttgcg
      481 ctttatggga gacaaaatgt tcaatgactt tttgaacact tgacttaaag tcgacaacta
      541 gagagtcgat tccttgagaa gtgccgtcag aatctaccaa aagcaaggca ttttgggatc
      601 ctttcatttc taataactga gcttcgtcga attttcctag tgcgatcgtt ccatctgtcg
      661 gaagatcact aatggaatcc cacgtttctt tcaataaagt aacgtttgct tcttctgcct
      721 ttttttcgat tcctagacga atagataaat aataaagatc ttcaagctct tccgtatcgt
      781 cgtaccactg gatcaggcga agcgtttgct tagtatttgc tgcattttta cgatgtttcg
      841 tataatttaa gcgttctgct gcttcaaaaa ttttttgctt tgtggctaag ccaacggaaa
      901 gatcaggatc atagttgaga actcttgaaa cagtagcagg agaaactcct gccaactgcg
      961 caatgtcttt aattgttgcc atttgtctct cctccttttt tacgtttata aaattgcagt
     1021 aaatcgttca aacgctgcct gtcctttttc atctcgcttg aatactccag cgtcttccag
     1081 tacacgtgca aaaacctgac ctacagattt ttgtacgaca tcggtgaccg tatctttcgt
     1141 caaatcagga tgttcttttt ttaattgttc tgcccacgga cgatgatatt cagccatctg
     1201 gttttcttct cctaataaga aatgctctac ttctactagt tctggtttca aacgaggtgg
     1261 taaaacagca agccccatta cttcgatcaa cccaatgttc tcctttttga tatgttggac
     1321 atctttatgt ggatgaaaaa tcccatcagg atgttcttct gaaacattgt tgtctcgaag
     1381 gacaaggtcc aactcaaagt aattgtccct tcttcgtgca attggcgtaa tcgtatggtg
     1441 tggtgtacca tctgctgaaa aagctctgat agatactgtt tcatccgaat atgctttcca
     1501 cttttccaat actgagttag ctgcttcaat caacaatttt ttctctgttg attgcaaacg
     1561 gatcacagac atcggccatt tcacgactcc tgcgatgact tgcggataat ctgctagttt
     1621 gataagtttt tctatcggtg cttttgccat aggaaattca tgacgtcctg cctgataatg
     1681 atcatgagaa agaatcgatc ctccaacaat cggcaagtca gcattcgaac caacaaagta
     1741 atgaggcaag acttcagtaa tccgaagcag tctagcaaat gtttcctttg tgattttcat
     1801 cgggcggtgt tgttctgata aaatgatcga atgttcattg taataagcat aaggagaata
     1861 ttggaatccc cagctttcac cgtctagatt cattcgaata atccggtgat tggtgcgggc
     1921 tggatagttc aagcgacctc tatatccttc gttttccatg caaagcatgc aggtaggata
     1981 gtctactttt accgcctcgc gttctgctgc gatttgtttt ggatcttttt ctggcttaga
     2041 taaattgatt gtgatttcca gttcaccata ctccgactct gccggaaatt tgatgttttt
     2101 tgcgatggct cttgttttga tgtagtcatt tttcttactt aaattaaaga aatactcagt
     2161 tgcttcctct gggcttttcg aatagtgctg tgcaaagaat gcattgacga ctgaaggtgg
     2221 cggtgtgaga aaatccatta attgcgcttc aaaaatctct tgttctgctg gcaattctga
     2281 aagaacacca ttttctcttg ccttagcgac aagctgatcc aataagtcta cagattcttc
     2341 cttaactggt cttacttcaa tgctttctag agcatcttct cctaccatcc ctaaaagacg
     2401 attctgtaaa tataagcggt ccatttccat ccatccgcca gcttcgatcg caagtgtggc
     2461 aaaatcacaa atcgtttgac tgattgacat atctcattcc cctattcttc ataaccattt
     2521 ggatggctga catgccaatc ccatgctgtt ttgataattg ctttgatatc tgtgtattca
     2581 ggtttccaac ccagaacctc tctcgctttt tcacttgaag cgactaatcg gctaggatct
     2641 ccagctcttc ttggtgctat ttctgctgga atttcttttc ctgtcacttc tctagctgct
     2701 tctagcattt ccttgactga gtatcctttg ctgctgccta aattgaagaa gttgctttca
     2761 tttccttctt tcaagtattc caaagctaaa atatgtgcag caatcaaatc ttctacttga
     2821 acataatctc tgatacatgt tccatctggt gtatcataat cgtctccata aacagccaat
     2881 gcttttcttt gaccaagagc tacttgtaaa atgattggga caagatgcgt ttctggtgta
     2941 tgatcttctc cgatagatgc atctgctttt gctccggcta cattgaagta gcgcaaagcg
     3001 acatagcgca tgccatatgc ttggtcacac cacttcatca ttttttccat catcaattta
     3061 ctttcaccat acggattttt aggattagct ggtgtatctt ctgtgattgg agactctttt
     3121 ggttctccgt aagttgctgc agtagaagag aacacgattt tattcacatt gaactcgtgc
     3181 atgacttcaa gtaagatttg cataccgtat acgttgttgt tgaaatacat caatggtttt
     3241 tcgacagatt ctcctactaa agagcttgca gcaaaatgga ttactccttc aatcggttcc
     3301 ttttcaaata cggaacgtag aaattcttta tcccggatgt ctccttcata aaaatgagca
     3361 tctggatgta ctgcttgttt gtgacctgtg agcaaattat ctaccacagc tacttgatac
     3421 cccttttgaa ctagctggtc tacagcgtgc gaaccgatat aaccggctcc gcctagtact
     3481 aaaattgtca taaaaaacac tcctttgatt agtaaaattg tgtttgttga ttatatataa
     3541 tttaaaaaaa cgaagtttac ttttatggtg aaaacaaaca aagtaaa
//
LOCUS       DAYYGS010000153         3531 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000153 DAYYGS010000000
VERSION     DAYYGS010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.160"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            45..1376
                     /locus_tag="ACHA44_002747"
     CDS             45..1376
                     /locus_tag="ACHA44_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HFM8905966.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            1342..1692
                     /locus_tag="ACHA44_002748"
     CDS             1342..1692
                     /locus_tag="ACHA44_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905967.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1784..2203)
                     /locus_tag="ACHA44_002749"
     CDS             complement(1784..2203)
                     /locus_tag="ACHA44_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFM8905968.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(2204..2548)
                     /locus_tag="ACHA44_002750"
     CDS             complement(2204..2548)
                     /locus_tag="ACHA44_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFM8905969.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            2898..3494
                     /locus_tag="ACHA44_002751"
     CDS             2898..3494
                     /locus_tag="ACHA44_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFM8905970.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT          928 a          642 c          643 g         1318 t
ORIGIN      
        1 tcatttcaag ttatagttaa agaaaatgaa aaagaaggtg tgtaatggaa aacgaaactg
       61 acgttattta tattcatcct caaaaacgaa tcgtttcaca gaaaagaaag tatttttatc
      121 ttggttttac aggtgttttt tttcttttta ttggcttgct ttctaatact cctactgata
      181 attggagtgg attgctaact attcttacct cgccaagcaa tcttttgaca gattattttg
      241 ctttaggtgt tttcggaagc gcattcataa atgtagggat tttgacctta ctttctgtgc
      301 ttttagctta tcggcacaag gtgattttaa acggtccctt atttgcttcg attctaacag
      361 ttaccggttt ctcttttttt ggcaaaaacc tttataattc tatctcaatc attttaggtg
      421 tgtatctata cgctgtattt gtcaataaac ctttttctca gtacatcatg atcggactgt
      481 ttggtagcgt attatcaccg gtcgttagtt atatcacttt tggtatgcgt tttcctttac
      541 tgataggtat tttattgggg aatttagctg gcatagcaat tggtatactt cttccgccct
      601 tagctgctca aactttagtt ttccatcgtg gattcacact ttataatatt ggattcacct
      661 ctgggttgat tgcgatgact tttactgctg ttttgaggtt attcagctat tcaatcgtcg
      721 aaaatacatt ggttttcaat gaatatcact ttcctctcat ttggattatt tttggctttt
      781 tttcgctgac agtcggaatt ggtttctatt ataattcctt tcggctttcg gggattcgag
      841 aaattttcga ttcatctgga aaattgacaa ctgatttcat tgctaattct ggtattggtg
      901 ctactttgat caacatgggc ttagttggtc tgatgctgtc ttcttatgtt ttgctggttg
      961 gcggtcagtt aaatggacct gtcatcggtg ctattttatc tgcggtgggt ttcagtgctt
     1021 ttggttgtca tttaaaaaac agttttccga tactagtcgg aatatttatt gcttctttgt
     1081 ttggaacttt tcatgagatc accagtactg gcatgcttgt tgcagctgtg tttgggacag
     1141 gtcttgcgcc cataagcggt ttttacggca gtttttacgg tgtaatagct ggtgtgctac
     1201 atattgctct tgtccataac gtcagcactt tgcacggagg actaaatcta tataacagtg
     1261 gtttttcaac tggtttcgtt gcaggtatcc ttgtaccgat actagataac tttaccgctg
     1321 tcagaaagga aaaaaagaca cttggaaaaa gaattatcaa aaagaatcat cgatgagctt
     1381 cttacttatt tatattccca tgaaataact gaaatacgta ttggagtaga atttgctgag
     1441 gaagggcttt tcatagagat acagggtacg gcagaaactc agccagaaga tttagatgta
     1501 cttgctgatt tgctgaacaa tcctcgggac cctagtatgg aaatctacta tcaagaacta
     1561 ttaggtaatc cccgacatga tcgggaagat tatcatctgt taggtagttt gattgatgac
     1621 gtggaaatca tgtatgccca gccgattatc aatattaaga tctttcgacg tgcttctcat
     1681 tcctattatt aaccctattt tctatttata aaagactgaa tcaaaaaagc agctcctgaa
     1741 agttcgatta aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc
     1801 cttcatacac ttcatctgtt acaggttcta aaaactctga attaccagca gtgatcgcca
     1861 aatgttcaaa ccaactgtct tttgtcgcac catgccaatg tttaatccct ttgtgcgtta
     1921 cgacgacatc tccagccatt aatcgtcttg ctggctttcc ttcttcctga taccagcctt
     1981 ctcctccggt cacaagtaag atttgatagc cgtctgtatg gacatgccag tgattgcgac
     2041 aacctggttc aaaagttaca ttacctacac caacagaaac ttcgggatca ttcactaaca
     2101 tattcagata gctttgcccg gtaaatactt gagcgtaagc ttcgtttttt tcacctgctg
     2161 gaaaaatcac gccattgtgt atttcttcgt gttttgtcat catttacgcc tctttcttat
     2221 cttcgctatt ttcttctaaa tagatttctt ttgctaaact aaaagctgac caagcttttg
     2281 gccattctac ataaaaagct aaatgggtaa tgatttctgc tatttcttca ctagtaatgc
     2341 cattttgctt gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta
     2401 atgcagatat ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt
     2461 gtccaaataa aacatcatca tttaattctg caaattttgg tgcaaaatct cctaattgat
     2521 ctcgtcctgc ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg
     2581 attcaactat ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa
     2641 tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag
     2701 ttgctaacgc tttttttatt ttaaaaaaaa caagttatta aataagtctt tagttggatg
     2761 tttttttgag atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc
     2821 atatcaaaaa atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc
     2881 taaggagttg atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca
     2941 ttatcggatt aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca
     3001 aagatttgaa tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt
     3061 ttgtcttcat ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg
     3121 cagctggtgt cctgatattt ggcccttatg ctggatttat ctataactat attggaatct
     3181 gcattggttc gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt
     3241 ccatgattag cgataagaca tataacaaat atattggctg gctggataat caaaaccgtt
     3301 ttgagaagct gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt
     3361 tgatggctgg attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta
     3421 aaccactgtc gattttctta tatagtatgg ccttgattta tggaggtcaa tatctaagtg
     3481 gtcttcttgg ataatattta ttttgtaaaa ttagtcattg attgacaatg t
//
LOCUS       DAYYGS010000154         3529 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000154 DAYYGS010000000
VERSION     DAYYGS010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.161"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..482)
                     /locus_tag="ACHA44_002752"
     CDS             complement(<1..482)
                     /locus_tag="ACHA44_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HFM8905971.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYSFEEKIP
                     HFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLYLDSTHIKANANKHKFT
                     "
     gene            complement(472..1671)
                     /locus_tag="ACHA44_002753"
     CDS             complement(472..1671)
                     /locus_tag="ACHA44_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8905972.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVRGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1801..3177)
                     /locus_tag="ACHA44_002754"
     CDS             complement(1801..3177)
                     /locus_tag="ACHA44_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8905973.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(3177..>3529)
                     /locus_tag="ACHA44_002755"
     CDS             complement(3177..>3529)
                     /locus_tag="ACHA44_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFM8905974.1"
                     /translation="LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVS
                     DAPIILADEPTGNLDSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSG
                     KKLKKVNKMNLEVE"
BASE COUNT         1082 a          677 c          519 g         1251 t
ORIGIN      
        1 ttcgtaaact tatgtttatt ggcgttagct ttgatgtggg tagaatccag atacaaatta
       61 tcctctgtta caaaacctgc tttaactgct tgttctaaaa tataggcaaa aatatcctca
      121 aacaccgtcg tttctctaaa tcggcgaaca taattctttc caaaagttga gaaatggggg
      181 attttctcct cgaaggagta gcctaagaac cagcggtaag cgacattggt atcgacttcc
      241 ttaatggttt gtcgcatgga acggataccg aataaatact gaataaacac caatttgatc
      301 agaatcactg gatcgatact tgggcgacct aaatcggagt aagtggcttc gacgattgga
      361 taaataaatt caaaatcaag cgcggcatca atctttcgaa cgagatgatc ttgaggaact
      421 agatcatcaa tcgtaagcat cgcaaattga tttctaccat tcgtattgtt tttagacatc
      481 atagctatca ctccttgcat tcgttttcat aaatctatta taaaagaaaa gactgtagac
      541 atcatccata aaatggactt tgtctacagt ctgcgcattc tcattagaga acactccctt
      601 ttttgtgact tttctgaagc tagagtgaat actttcaact gcattagtag tgtacatgat
      661 ttttctaatg gcgctgccat aatcaaagag ttgttcaaca tgagaaaagt ttcgtttgca
      721 tacatctact gcaccagaat aatgagacca ccgattttga aaactgtcga aggcagcgtg
      781 tgcagcgttt aaagaagaag cactgtagaa ttttttcatg tctcgacaga cttccttata
      841 atccttactt gggaggtatc gtaacgcatt tcgaacaaga tgaacaatac agcgctgcac
      901 aatgacagaa ggaaaaattg cttttgcgcc ttctttgaga ccggaaacac cgtccatcga
      961 aatgaaaaaa acatcttcta caccacgtac tttcagttca tcaaatactt gcatccagcg
     1021 atttttagat tctgtttgat tgagccatag gcctaaaatc tccttgtttc ctttaagatc
     1081 atagccaaga atggtgtaca cagcatattc tttagcttca tagttttcgc gtaaagtgac
     1141 gtacatacaa tcaacgaata gaaatgcata acacttggct aatggccgag cttgccattc
     1201 ttccaattca ggaaggacgg catccgtgat atcagaaatc atttcgtggg aaatatcaaa
     1261 gccgtaaata tcttcaacgg ttgcggcgat atcgcattga ctcattcctc gtgcatacat
     1321 ggaaagaacc ttaccttcga tgtcggagac atctcgtttt ctattaggaa tcaactcagg
     1381 ttcaaaagaa gcctcccggt ctctgggaac atcaatgttt actttaccaa agctcgtttt
     1441 aagcgtctta ttgccataac cgtttcgtcg attgtcatgt ttctttggct ctttagaatg
     1501 ggcgtcatac cctaaatggt tattcaattc tccttgaagc attttttcaa ataagggacc
     1561 aaacacatct tttaaaacat cttgcatatc ctctacagat tcgggttgat aagtattcaa
     1621 aatggattca gctaacttct cagcatcagg atttcttttc tttctagcca tcgtgtaatc
     1681 acctcttgat cttattttag aaaaataaaa accgtgaagc aaccacccgc ctaggattga
     1741 tggttactta cacggtttat attacactct catttttggt gctaaaaagc accgaaaatt
     1801 ttaattattg tcaattaata attcttttgg attccttctt aatatattga atgaagaaat
     1861 aatcaatgat acaagaagta ctaaactcat aaatataact acataaatca tgaatttagg
     1921 taatacattg atatctaaac ttgataatgt cttgttaaat ccttcagctt ctgccccacc
     1981 acctaattgg ctagatgcag attgtttagc tatttgttta gctatatcac cagtaacctt
     2041 attcaatata ttattcccaa gcttgtcagc tgtatattga gctaagaaat aggaaccaac
     2101 gaatgcgggg attgatataa ataccatctc aattaggaat tgaccaaaaa tttctaattt
     2161 tgatatacct aatgatagta atactgctat ttctttcttt ctagcattca tccataagaa
     2221 taataataat gaaactacaa ctcctgcaaa tattaatgag ccaaaaaata atttatttga
     2281 aattgagtaa atacctgata tagattgttg taatgcaggg taatttgatg aacttttaat
     2341 caaattatat tctctccaat ttatatctaa tttcccaaga tcttttatta cactatcaag
     2401 attcttatca ccttttacaa agaatgttgc atcttggtat acagctgtat cttcggtatt
     2461 accataaact ttagcagctg tatgaatatc agtgattaat gtattttcgt atagttcttg
     2521 tgctgcactt actccaccgc tattatgccc atcgaataga cctttaattt ctacttctac
     2581 tgtttcatct gcacctttct cattatcagc atcaaataaa ttagatttta tttttatctt
     2641 atctccaact ttaaggttat ttttttcagc taaatcttta tgcattaaga ttttattttt
     2701 atcttcattt tctaaatgct ttccttctac taatttatat gcttctgata caaattttgt
     2761 ttcttttgtt gagtcgttaa ccccagttaa cataactgtt cttttaaaat tctttgctct
     2821 ttcaggtgat tgattcgcaa gagtctcttg agtttcaata atatcataat caactaaatc
     2881 tgcaacactg tttattcttt ttacatagga gtctatacta tctgtttgag atatcttttt
     2941 aatatcttca ccttttacat ttcccccacc tctaggtgtt cctggattta cttgtctatt
     3001 tatctccata gaaaaactat ttgttatatt agcaaatgtt tcttttgaag ctctgtccgt
     3061 agcatcttta atagataaac taatgagact taaagttgac atagataaaa taactaatat
     3121 gataattaat gattttaaac tttttctagt tacataagca aatgcatttt ttatcattat
     3181 tcaacctcca aattcatttt atttactttt ttcaactttt taccacttag ttctaaaatg
     3241 atatccgcag aatctgctac ttccttacta tgtgttacaa ctattacaca tttatttcta
     3301 tctttagcta atgtctttaa tatattaatt atttctccag cagtaacact gtctagatta
     3361 ccagtaggct catcagctag tattattgga gcatctgata ccaatgctct agcaatagct
     3421 actctttgtt gctgtccacc agataatttc ataacatttc tttttatttg ttttttatct
     3481 aaacctagtt caaacaagat actttcatct gctgatttat ttactaatc
//
LOCUS       DAYYGS010000155         3418 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000155 DAYYGS010000000
VERSION     DAYYGS010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.162"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..563)
                     /locus_tag="ACHA44_002756"
     CDS             complement(<1..563)
                     /locus_tag="ACHA44_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8905975.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDS"
     gene            724..1413
                     /locus_tag="ACHA44_002757"
     CDS             724..1413
                     /locus_tag="ACHA44_002757"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFM8905976.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            1427..2929
                     /locus_tag="ACHA44_002758"
     CDS             1427..2929
                     /locus_tag="ACHA44_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFM8905977.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            2942..3418
                     /locus_tag="ACHA44_002759"
     CDS             2942..3418
                     /locus_tag="ACHA44_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFM8905978.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1115 a          487 c          631 g         1185 t
ORIGIN      
        1 aatgaatcat aagtatcttt cgttttccca gataaagaaa aaatgattac taaatcatta
       61 actgttaata aagaagcgac ttgggcttga aaatgagggt ctagtacagc ttttgcttga
      121 actcctacgc gaagaaacat attttctaaa tcaagactag tatttccact agatcccact
      181 ccaaatatat atatgttttt tgattgagtg atcattttaa tggcttcatt caatttttct
      241 tcgtctaaaa gcctaatagt ggaatagaga gcttctgtta aactattagc aatttcatca
      301 tagtacttta cactagtcgg atgttctttt ttttgactaa agtcttcctt tgcaatttca
      361 atttttaaat cagaaaaacc agaaaaacca attttttgac aaaagcgaat aatagtagca
      421 tcgccaactt ttgtgttttt cttaatatca ctcatcgtac tataaataat agttttccca
      481 gaatttaaaa taaaatcagc gacttttttt tcagacttcg ttaaaaatgg ataattttct
      541 ttaattaaat agatataact catacaaact cctctacaat ttcaactttt ctccattata
      601 ctccaaaata ataaaaaaaa ctcctaaata ctccattttt atctttactt ttatgaaagc
      661 ggatgctata ttgtttttgg agttgttatc caaaaataat aaaaaattgg agtgtttagg
      721 ataatggata aaaagaggtt tttagatctt atttcaaaag gtttgattgt ttcctgtcaa
      781 gcattacctg gtgaaccatt ttacaaagag gaagaaagtt taatgcctta ttttgctttg
      841 gctgctcaaa gggctggagc agttggtata cgtgcaaatt ctgttcgaga tattattgaa
      901 attagaaagt gtgtatcatt accaatgatt ggaattatta aaagagatta tccaccagaa
      961 tctccattca ttactgccac tatgagagaa gtagatgaat tagtagaaac cggtgttgag
     1021 gtaatcgctc ttgattgcac tcttagaaat cgacatgatg gactatctat aaattctttt
     1081 attactaaaa taaaagaaaa atatcctcat cagttattta tggcagatat ttctacattt
     1141 gaagaaggca aaaatgcata taaagcagga atagattttg taggaactac tttaagtgga
     1201 tatactgagg aaagtaaaaa acaagatggg ccagatatag atttgattaa aaaattggta
     1261 gaagaaggga tttgtgtaat tgctgaggga aggattcata ctccagaaca agcaaaagag
     1321 ataaaaaaaa taggagttgc aggtattgtt gtgggtggtg ctattactcg accacaggaa
     1381 attgcatcaa gatttgttaa agcattaagt tagggggaga aataaaatgt ttaaaaaatt
     1441 ttcacaatta ggacgcgctt tcatgttacc aattgctata ttaccagtag ccggattact
     1501 attgggactg ggaggagcgt taacaaatga gagtgctata aatgcttatc catttttaga
     1561 tcaaccgtgg ttgcatacaa ttttaagtat tatgagttat gctggaaatg cagtttttgc
     1621 aaacctagca ctaatttttg caattggtat tgctgtagga cttgcaaatg gagataaagg
     1681 aacagctggt ttagctggtg gagtagctta tctagtatat actgcaacta ttagtgggtt
     1741 tcttgaactt ttttcagcaa aagatgcaac actagataca ggtgtagtgg gatcaattgt
     1801 tgttggtggt actgttgcat ttttacacaa tcgttataga aaaattgaat taccacaatt
     1861 cttaggtttt tttggaggat caagatttat tccgattgtt tctagtttag ctgcaatcat
     1921 tattggttct ttcttttatt taatttggcc accaattcaa aatggtttag tagttgctgg
     1981 tgaacaaatt gcccaaatgg gtagtttagg aactttttta tatggttttt tattgcgttt
     2041 aacaggtgct gttggactac accacaccat ttaccctttg ttctggtata catctttagg
     2101 tggtaccgaa actgtagctg gatcaacaat tgctggagcc caaaatattt tctttgccca
     2161 actagctgat ccaaaccaca ctggtctgtt tacttatggt actagatttt ttgcaggaag
     2221 atttgcaaca atgatgtttg gattaccagc agcatgttat gcaatgtatc gtgcaattcc
     2281 taagaaaaat cgtaaaaaga atggtgggct ttattttagc ggggccctta catcattttt
     2341 aactggaatt actgaaccag ttgaatatat gtttttattt gttgctcctt ggctttatgt
     2401 gattcatgca ttcttagatg gattatcttt ttattttgct gatatcttaa atattcgtgt
     2461 agggaattcc ttttcaggtg gtttgatcga ctttttatta ttcggtgtac ttcaagggaa
     2521 tgacaaaact aattggttaa aattaattcc ttttggtatt gcatgggcat ttatatattt
     2581 ctttgttttt acattcttta tcaaaaaatt taaggtagct attcctggaa tggaagaaga
     2641 atcagaagtt cattctgaaa aagtaataaa caaaaataca tcattacatg aagagtctct
     2701 tatcattatt gaagctcttg gtggaaaaga aaatattgag tctgttgaag cttgtgcaac
     2761 tcgtttgcgt gttgctgtta cgaatgggga gattattcaa aaacaaacaa taaaagacac
     2821 tggtgcaact gctgtcttcg aagtgaaggg tggcatccaa gctgtttttg gagggaaagc
     2881 tgacttatta agtcaagaaa tcaatcaaat attaggaaaa gataattagg aggtttttaa
     2941 tatgtttgaa attttcaaaa agaaaaaaca agaaactttt tatgcacctt gttccggaaa
     3001 aatcatttcc atcacagaag taaatgatcc tgtcttttca caaaaattac taggtgatgg
     3061 ttttgccgtc atacctgatg gttcaaaaat ttatgctcca ttggctggtc gagtaacaaa
     3121 tatatttcct actaaacatg ctattaatat aaaaagcaac tcaggcattg attatttgat
     3181 tcatattgga attgatacag ttgaattaca aggagagcct ttttcgattt gtgttcaaga
     3241 aaatgatgag gttactcctg aaacctcgtt ggttgatgtg gattttgaca aaataataaa
     3301 atctggtaaa gatacttctg ttatagttgt ttttccggag aaaaatcagt tcaatgattt
     3361 aataatagat aatggtcgaa ttgctcatgg gaattcatgt ggaaaaatca ctttttaa
//
LOCUS       DAYYGS010000156         3404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000156 DAYYGS010000000
VERSION     DAYYGS010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.163"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..671
                     /locus_tag="ACHA44_002760"
     CDS             <1..671
                     /locus_tag="ACHA44_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HFM8905979.1"
                     /translation="DKYSMQCLHRQTANFIIFKGEDLIGTTQDFSYFVINAVSKTEGE
                     MGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEEFERWRQEQEHSFQDWREGQESD
                     YLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVRHASISERLLADMDYLYQKLRAT
                     LFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPIEEGALIIATVDDPKQNVFTLEK
                     VGVI"
     gene            671..1291
                     /locus_tag="ACHA44_002761"
     CDS             671..1291
                     /locus_tag="ACHA44_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905980.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            1295..1744
                     /locus_tag="ACHA44_002762"
     CDS             1295..1744
                     /locus_tag="ACHA44_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905981.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKVKLDGLANITFEKVGTV"
     gene            1744..2238
                     /locus_tag="ACHA44_002763"
     CDS             1744..2238
                     /locus_tag="ACHA44_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905982.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            2252..2683
                     /locus_tag="ACHA44_002764"
     CDS             2252..2683
                     /locus_tag="ACHA44_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905983.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            2685..2822
                     /locus_tag="ACHA44_002765"
     CDS             2685..2822
                     /locus_tag="ACHA44_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HFM8905984.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITDEEADKIINKDKSA
                     S"
     gene            2859..3152
                     /locus_tag="ACHA44_002766"
     CDS             2859..3152
                     /locus_tag="ACHA44_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905985.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            3149..3373
                     /locus_tag="ACHA44_002767"
     CDS             3149..3373
                     /locus_tag="ACHA44_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFM8905986.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
BASE COUNT         1222 a          511 c          756 g          915 t
ORIGIN      
        1 ttgataaata ttccatgcag tgtttgcatc gacaaacagc caatttcatt atatttaaag
       61 gagaagactt gattggaaca actcaagact tctcttattt tgtcattaat gctgtttcaa
      121 aaacagaagg agaaatgggt tcttattggc aatcaatcga agatttaatt gcggatatga
      181 cggactttat taatgaaaat aaaggcgatt ttacggactg gatgaatgaa aggaaagaag
      241 aatttgaacg ttggagacaa gaacaggaac acagtttcca agattggaga gaaggacaag
      301 aatcagacta tttggcatgg tttgaatcaa tcaaggatat tttaaagacg attgatccag
      361 gtggagtcat gttagccgaa ctaacggatg cgcgagtaga tattcaagga gttcgtcatg
      421 cgtctatttc agagcgtttg ctggcagata tggactattt atatcagaaa ttgcgagcaa
      481 cgcttttcac gattgaatac ggggagattg aagtgactga tattttgcag gatgatctct
      541 tttcagataa tcatgaagtc gaaaaagttg gaactgtagc attcccgatt gaagaaggag
      601 ccttgatcat cgcaaccgtt gatgatccaa agcaaaatgt gttcactctt gagaaagttg
      661 gggtgatcta atggctaaaa ctaaacgaat gatggaaacg gatgaaaaaa cgggtgtaca
      721 acgccaattc tttccaatca cacatgcttc cgcagttctt gggttagaag aaataatagc
      781 aggagaagca acagttttat ctgttaatgg aaaaattgga gccgtcgtca ttactaaaga
      841 agatttggga ttagagaatg ttctcacaga attaccctat gcaagtgaag aagatgacgg
      901 cattatcacg gcagaaatgt atcaaaaaat tttaaacagt ggagaaggtg actacgtgtt
      961 gccagttgcc actatcgacc gtttgggtgg cataaaagtt ggtgaattat tgacgattga
     1021 tgagacaggg aaagtctctg cagtcagaca atctgatgtc aatttttcgc tggagttaaa
     1081 agaaaaactc gattcactga aaaattatac tgctggagaa aacatcacta ttgatgaaga
     1141 tggaacaatt agttctacag gtggtagtgg gactggtggt gtcaatcagt catatgtaga
     1201 tcaaaaattc caagaagctg taaatcaagc agaaaattac acaaacgaaa gaattccaaa
     1261 ttttactttt gaaaaaattg gggaggtata gagaatgaca gatatcgtaa aaataaaaca
     1321 aagcaatgta caggtttatc cacaaactca ttggaatgct atagaaggca aaccaacaac
     1381 ggtgaaggga gataaaggcg atcctggcca agcagcaaca attaccatag gaaccgtttc
     1441 tagtggttca accgcttctg ttacgaatgt aggaacatca tctgcagcta gatttaattt
     1501 tgttttacca aagggagata aaggtgatcc tggaataaat gccacaacta cagctgtagc
     1561 tacaactaca gcaaatgggt tgatgtcgtc cacagataaa actaagttgg atggaattgc
     1621 agctggagca cagaaaaatc caggtaatgc tacaactacg acagcaggtt taatgtcagc
     1681 aaccgataaa gtgaaacttg atggattagc gaatattaca tttgagaaag tggggacggt
     1741 ttaatggctg atattgttca attaaaagaa gacggagttg ctaaatactt aaaaacgcat
     1801 gtagaagcta ttgatggtaa agaagcattg gtgcagacag atggagacca agccattgca
     1861 ggacataaga atttttcagg ttctgtaaca atcaataata aacgtgtttt gacgactgat
     1921 gataacagat atgaagtggt aaatctggtt gtcactaacg gtaatacagg gacagcaaag
     1981 ctttatcgtg aaggaaaaac agtttctata tattttgtgg ctttaaacgg aaaaagtagt
     2041 ggcgggaatg attcggttat tttaactgtt ccagaaggct atcggacacc aattagtttt
     2101 gaacaactgg ttgggtcaat agaccgttct actttgaaca gtgctcaact atctattggc
     2161 gcagacggag ccattaaatg gagaagaaat tcaagttatg gatcagctta ttcatttgtt
     2221 atcacttatt caatttaagg gaggaaatct aatgaaagta gtttacaaat caatcaagcc
     2281 ttacggattc gagcaaatca ttttgaacaa tcaagaaaat atccctgaaa actgtacaga
     2341 gattaaacca ccagttccta actggagacc aagatttgat tttgataaaa aacagtgggt
     2401 tgagttagct actgaagaag agaaaaaagg gaatgctgtt gatagcgtag atgaattggc
     2461 taatattaag gcattatacg aaacgctgaa ggcagaaaat gatgaattga aacagctaaa
     2521 ttctaaagca atgctaaaca atgtagcaat caagcaagaa aatgtcttat tgaaagaaaa
     2581 gtcagaaagt ttagcgcagt tgaattcaaa aacgatgctt gcttctgtac aaaataccaa
     2641 ggaaatcgaa gaaatcaaaa aacaattaca aggtgggaag taacatgtat tcatatgaag
     2701 atatcaaact aatgtatgac tggggctgtt tcactaacga acaagtaatg gttttcgttc
     2761 cattatgcat tacagacgaa gaagcagata aaatcattaa taaagataag agcgcatctt
     2821 aattgatgcg tttttattta aggtaaagga gttgtcacat gattaattta ggggaatggg
     2881 gaatgatagc aggatcaata accgctatcg tttctttgat tttattagta attagaccga
     2941 ttgctgcatc tttctcgaaa attactgaga ctctttcaaa agtaagccac aatttagatt
     3001 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
     3061 agaaacacga tgaaagatta gatacacata cagaaaaatt ggtggaacac acgcaacaaa
     3121 tcaaaacttt gtttagggag agaagaaaat gaataataaa acgttcgaag tactaaaatg
     3181 gttcgcactg gtaattattc ccgcactagc tactttcgtg gggttagttg gtaaagcgct
     3241 caattggcag tacacagata tctgtgttgt catcattact ggttttggcg cgtttttagg
     3301 gagtgtgttg ggtgtatcaa atcgaaccta caaaatgttc tcggctgaaa gcgaagaagg
     3361 aggaaacaaa tgaaaaagaa aattactatt actgcgatga gcct
//
LOCUS       DAYYGS010000157         3388 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000157 DAYYGS010000000
VERSION     DAYYGS010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.165"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(63..2555)
                     /locus_tag="ACHA44_002768"
     CDS             complement(63..2555)
                     /locus_tag="ACHA44_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HFM8905987.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(2562..3029)
                     /locus_tag="ACHA44_002769"
     CDS             complement(2562..3029)
                     /locus_tag="ACHA44_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HFM8905988.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(3165..3238)
                     /locus_tag="ACHA44_002770"
     tRNA            complement(3165..3238)
                     /locus_tag="ACHA44_002770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3202..3204),aa:Arg,seq:acg)
     gene            complement(3296..3369)
                     /locus_tag="ACHA44_002771"
     tRNA            complement(3296..3369)
                     /locus_tag="ACHA44_002771"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3333..3335),aa:Pro,seq:tgg)
BASE COUNT          924 a          750 c          557 g         1157 t
ORIGIN      
        1 cttgtcacaa cctcttaatc aagtcttact attataaaaa ttcagctaca caggccaagt
       61 aactataggg aacctactgt ttccttttta ggtccgcgaa cattcaatgt gatcttacct
      121 tttgaagcac ctactgttac gtggtcacct aaacgaattt ctccgccaag caaagcctcg
      181 ctcaatcgat cttcaatttc tttttgaagt gcgcgacgga ttggacgtgc accatattca
      241 ggatcaaatc cagctttccc gatcacatcg attgcagatg gtgtgatttt cacttgaata
      301 tcttgttctt tcaatcgttt gataatcgct ttgctcatga tttggacgat tgtatggatt
      361 tcatctttac tcaatgaatg gaagacgaca gtctcatcga ttcgattcaa gaattcaggt
      421 cggaaagctt tctttaactc ttctaagata cgtttttgca tggctttgtg atctttagta
      481 acatcttgga cattgaatcc gacatttttt tcttctcgaa tttgtgtcgc tccgatattt
      541 gaagtcataa tcatgattgt atttctaaag tccacttttc tacctttaga atctgttaaa
      601 tgaccatcat ccaatacttg cagaagtaaa ttgaaaacgt ctggatgtgc tttctccact
      661 tcatctaaga gaatcactga atatggtttt tgacgaattt tttctgtcaa ctgtccacct
      721 tcatcatacc ctacgtatcc tggaggagac ccgattaatc ggcttgtact gtatttttcc
      781 ataaattctg acatatcaac acggatcagg gcatcttcac ttccaaacat gacttctgaa
      841 agtgctttgg ccaattctgt ttttccgaca ccagttggtc caaggaacat gaatgaacca
      901 attggacgat caggatcttt caatccgctt cgtgcacggc ggatcgcacg tgaaactgct
      961 ttgacagcct catcttgacc aacgacacgt tcatgcagaa gtccttccag ttctaacaag
     1021 cgttcgcttt cttttttctc caactgctgt agagggacac cagtccactc cgatacaact
     1081 gtagcaacat cttcttctgt tacacgatca gaataacctt gctcctgttt tgcttcaatt
     1141 gcagaaactt tttgcagctt tttacgtagt gctttttctt gtctgcgtaa ttgagcagca
     1201 ttttcaaagt cttgtttttg gatcgctgct tccttttctt gtactagttc atcgatttct
     1261 agtttgatca cagttgattt tgttaagtga tcggtttgat ccaaacggac tttcgctgct
     1321 gattcatcga tcaaatcaat tgctttatcg ggtagttgac gagaagtgat ataacgaaca
     1381 gaaagattga ctgctgctcg tacagcttca tctgtgatct ccactccatg gtgctcttca
     1441 tatcttgtac gtaatccttg aagaatcact tctgcttctt caggagttgg ctcatctact
     1501 tgaatgcgag caaaacgtcg ctctaatgca gaatcttttt cgatatattt ttgatattca
     1561 tctagggtcg ttgctccgat cgtttgtaat tctcctcgag ctaacgccgg tttcaagata
     1621 ttcgaagcat caattgctcc ttctgctcca cctgcaccaa ttaaggtatg caattcatca
     1681 atgaataaga ttacttgacc atcattatag atttcgtcga tgaccttttt cattcgatct
     1741 tcaaattcac cgcgatattt cgtaccagca accaaagatc ccatatcaag catcatcagg
     1801 cgcttttctt gcatgtcttc tggcacttca cctaaaatga ttttttgagc taacccttca
     1861 gcaatggctg ttttgcctac accaggttca cccaccagta cagggttatt ctttgtccgt
     1921 cggctaagaa tctggataag ccgtttcact tctctcgaac gaccaacaac tgggtccagc
     1981 ttgttttctt ttgctaattt cgtcaaatca cgtgctaatg aatctagcgt aggtgttcct
     2041 tgcggtgttt gccgtttatc ttgtacattt acatttctac gacgattcat tccttttcca
     2101 gcagaatcac cagctagccc cattttttgt ttcagaactt gacgggcttt tgaaaggctg
     2161 atacctaagt tgatcatgat tcgagtagcc atgatacttt catcttttaa taagctcatc
     2221 aaaatatgtt ctgttccaac tttttgagaa ccagttttct tcacttcgct gctagctaaa
     2281 gcaaaaactt gtttcattct tggtgaatat ggaagaaaga ctcctttagg gtaagaacgg
     2341 ataccaccat aaccaatcag atgttcaatt tcttcataaa tttccgtttc attcaaatcc
     2401 atttcacgta atacttttcc agcaattcca ttttgctcga ccactaaggc tagtaaaaca
     2461 tgttctgaac taactgattg atgtttcaac cgttttgctt cttcttgtgc aatttctaat
     2521 actgcttggg ctctttcagt aaaaagttct tccatctaat ctcacttccc atcttcataa
     2581 cttaaacgtt ccagaaatgc tcggagaatc cgagcacgtg tatgatcttc atattcattg
     2641 aaattcaatg tgttttttgc aattgcactc aacatcaagt ttccttcttt ttttgtaatt
     2701 atttggtctt cataaagttt ttgaataata gcaaaagcat ttttctcact gattgtatca
     2761 tcaaataact gatttatctg ctcaagtaat tgttttttat ctgaaatcct tacttttgca
     2821 attcgtatat aaccaccacc accacgtttg ctttctacta gataaccacg ttggatggta
     2881 aaacgggtat taatcacata gttgatttgt gaaggaacac agttaaagag gtctgccatt
     2941 tcaatccttc gaatctcgat catttcacta ttttccagaa ttttctttat atatgattca
     3001 attaaatccg aggtattctg atgtgccatc ttattcattc cctttgacta atattgacct
     3061 taattataac gaatttgata atagaaagaa agaagaacgc tttttgttaa ataaatcctt
     3121 tagaatcaac aaaaaaaaat acggaatata ctattccgta tcgacgcgcc caagaggagt
     3181 cgaaccccta accttttgat ccgtagtcaa acactctatc cagttgagct atgggcgcat
     3241 attcaattta aaaaaattat aaatcatttg aaaacaaaaa gcaagataaa aaaaatcggg
     3301 aagacaggat tcgaacctgc gaccccttgg tcccaaacca agtgctctac caagctgagc
     3361 tacttcccgt ttaaaaaata aaaaagac
//
LOCUS       DAYYGS010000158         3340 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000158 DAYYGS010000000
VERSION     DAYYGS010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.166"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            93..1133
                     /locus_tag="ACHA44_002772"
     CDS             93..1133
                     /locus_tag="ACHA44_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HFM8905989.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            1957..2289
                     /gene="traE"
                     /locus_tag="ACHA44_002773"
     CDS             1957..2289
                     /gene="traE"
                     /locus_tag="ACHA44_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFM8905990.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            2908..3249
                     /locus_tag="ACHA44_002774"
     CDS             2908..3249
                     /locus_tag="ACHA44_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFM8905991.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1243 a          456 c          638 g         1003 t
ORIGIN      
        1 aatgcgtttg cattttttat ttatctttgc tattttcgaa aagagtaggt aattttacat
       61 aaataaaaca acgtgaaagt agaggatcta caatgactga ttttaaattt tttaaggcgg
      121 atagagttta caacgaatta ttttatcaat ttccaaaagt ctttattgtt tctgacgaat
      181 acaaaaaaat gaaagattca actaagattg cctatatgct tttaaaagca agattagaga
      241 tcgcaatcag caaacggcaa atcgatgaag aaggtaatgt ttattttact tatacgacaa
      301 atgaactatg tagagtatta aactgccaaa aacaaaaagc gatagcaatc aaaaaagagt
      361 tggaatcctt tggtttatta ttacaaaagc agatgggatt taacaaacag ttagggaaaa
      421 ataatcctaa tagactatat ctagcagaat taaaagtctc agaaaatgat atctacttac
      481 tcgaaaaatt tgatagagag aatagggaaa acgttgataa atcagagggt atgaaaatca
      541 tacccaccct cgacgaaaaa tcagacgctg aatcccttgg ggctcaagag ggtatgaaaa
      601 tcataccgtg ccaaaacgtt gataaatcag agggtatgaa aatcatacca gaacttaata
      661 ataatatatt agacactaat agacacaata tagacactga aaaagaccgc ctacaagatc
      721 aattgttgtt agacaatttt gagacaatta tgacaaacga cagcattgct acgtttgtcc
      781 ctgaacgagt attaaatttg ataaaaacat tttcttcaag ttacagtgaa gctcaaaaaa
      841 ccgtccagac tattcataat gcaaagaaaa aagctgaaat agaaagtgat atttcgatag
      901 tttttgaaga actcgatagt tattatgtca atgcagaaca agaattatac acgacactgt
      961 taaaagccta tcaaaaatta aaaaccgaaa aagtcgaaaa tatccagaac ctgatttttg
     1021 tctatgtaaa aaattggttt atcgaaaaac caatagctgc taaagtatca agtgaaaaac
     1081 gtttgaatta tgcaagctcc ccaagcacta ttacgaaaga ctggttagag tgaaacttta
     1141 ctgacaaagt gattgttgct tcatggagta acattaaaat taaaaatttt ttattattct
     1201 aattatatta cgaatattac aaaataatag ctattttaat agaatttata agttaaaata
     1261 ttatgataaa aatcacaaac gtagcctttt acgtcataag tgttaaaaga aataattaat
     1321 ttcaaatata gaaaagggaa cgtatgtttg catactcctt ttttttgagt tataataata
     1381 cgcgaagaga gataatttca ttatttaaac aagctaaaaa tcttgatatt acttcttttc
     1441 aggcaatacg aatattattc gcttacattt tttgtataaa tttaattgtc tgctatgctt
     1501 gtgatgaatt gattttcgtt ttgcactttt ggatgataaa tttggacaat tgaaaataca
     1561 aaaaaaagaa aggcggtatg aactcggaag gctggtaacc ttcgtcagcg ttcatacacg
     1621 gcattaagat ttggccctta atgcgttaac ctttcagtgt tacggtatta cccgcaatgg
     1681 atttagtata acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa
     1741 atacgtgata ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc
     1801 tctttgccgt atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct
     1861 tttttgtttg tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt
     1921 gttaaaaata tttaaagaaa ttgggtggtt tattttatgt catatacagg aattttgagt
     1981 ctcaaagata tttgtcacta cggtaaacgg tgtaccgcta cagagaaaat cacgaagaaa
     2041 ctatcaactg gccaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
     2101 aaagtctcag aggaaatgat ttattacatt gggaaacaaa agcaaataat tttaaaagat
     2161 cctatcccct taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
     2221 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgtttg
     2281 atcggctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
     2341 attgtcgaga cttctaaagg aacttagagt cactagaaaa ataaggagta gacttgatct
     2401 atcaacaatt cccttcgggg agtgacgggg taggttttaa cgattggtaa cgtaaaaaat
     2461 tggtcaatcg agtaaatgcg cttgtttgga tagtggtgaa atgaatttgt gtaaataata
     2521 agcgcagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gtctagatta
     2581 atcatgagca gctactaagg gatctagact ttgacgcaaa ctcatggaat aaggagtagg
     2641 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
     2701 acttgcgagg acgttgatta ttggatcaat cgagcattta ccgctgtcta ctagggtttc
     2761 tcgtttcgga cgccataaac gagtcgaaat tttttatgta tttttaagta aacgaaaaac
     2821 gataaaatat gaacaagtcc tgaataaagt gaaaaaattg atataaaaca tagagaaaat
     2881 gaaatttacg taatggagga atgacaaatg aaaaattatg atccgaatat acgattgggc
     2941 acacatacta ttaaggtttc tttccaacgt tgggattaca aaggatttgt gacgtttaga
     3001 aggggcggaa attgtaaagg attagatgtt ttagcattag acgaggatga tttatacgat
     3061 caaacattga ctgataatcc aattggattc ggcttgctac ctgaagatga tgaaggtaat
     3121 gaatggttta aaatgacgct tatgaatgat aatggagacg aattatctgt tgaagatact
     3181 tggtcatatt tgagtgatta tattgtcagc tttgaaatca ttgaatttgt agcggataaa
     3241 gaagaataaa aactggagga ataaatttta cagataaagg cttagaagga gtcacgacga
     3301 ttaataaaaa ggtagtatta ccttaggcat tgagtaagga
//
LOCUS       DAYYGS010000159         3322 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000159 DAYYGS010000000
VERSION     DAYYGS010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.167"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(9..284)
                     /locus_tag="ACHA44_002775"
     CDS             complement(9..284)
                     /locus_tag="ACHA44_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8905992.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            516..1250
                     /locus_tag="ACHA44_002776"
     CDS             516..1250
                     /locus_tag="ACHA44_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8905993.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            1289..2431
                     /locus_tag="ACHA44_002777"
     CDS             1289..2431
                     /locus_tag="ACHA44_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8905994.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            2697..>3322
                     /locus_tag="ACHA44_002778"
     CDS             2697..>3322
                     /locus_tag="ACHA44_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8905995.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT         1212 a          567 c          575 g          968 t
ORIGIN      
        1 ttgtttactc aacctttata ccgttccatg aaactatcaa tatcagattt tttaaatcta
       61 atgaccgtat caatttttat aacaggtaac ccgtattgag aaacatattt tctaatcgtt
      121 cctactgata cacctagata ttcacttgcc tgctgatagt ccatatactc tagagtgact
      181 ccagaaataa tgtcttctag cataggcttt agcactgtaa ttatctcttt cacttgttgt
      241 tcaacttgta tagaaacaat gctatcaatg atttgtagag acatatttcc ccctcctttc
      301 gtgattgctt gaacaaattt tctaagaatt atcttcgtat tttttgctca aaataaaagt
      361 attttgttat taaccgatta atcaaacaaa aaatgattac cagttcatcg acatacacat
      421 aatacctcga taaaaaaggg gtgtcaatag ttttgtaaaa tttatttaca acaccccttt
      481 tttgaggtat actaatttta ggaggtgtgg tcaatatgat aaaacgtcca ccaataaatt
      541 atttagaacg aaaaaagata ctcggaacaa aaataaaagc tataagaaag agtaaaaaat
      601 taacacaacc agcatttggt cttatgatta acaacggtca attaattgac aaaaagacta
      661 tctacgaatg ggagaaggga acctatttac caataccaga gcgattatcc cgaattgctg
      721 atttgggaaa tatgagtata gaggagttag tttgtggcaa tgtagaagaa tatattttgg
      781 gaattattct ttatagagat agcattgtac tagatggtat aacatttcca gataaaaacc
      841 tgttccagca tcttcgtcaa cagttcccac cagtacactc taatttagat acatggctgg
      901 atagatattc caaattagaa ccagagatgc aagaatttat tgctaacaag acttgtaata
      961 aagttaaaaa tgaaaaaatt agtcttttca atatcttaaa aatagaagaa ttattcatca
     1021 atgctattgt agaagaattt gacaataaca ttcttttcct cactagcagt atagaagaac
     1081 tactagaaag aatggtagat gaatggctac ctattcaatt gaaagacatg tcttatcccg
     1141 aagaagctgt gagagaaatt actgataata taaacaagct agaacaaacg atttcatcaa
     1201 ttgggaaaaa atacactaag aaaaaaatga agggaggtga taccatctag tggtaagtac
     1261 tccttgtaaa ctaagaggag atgatttcat ggcaaaaata tcaaagtaca aaaagaaaaa
     1321 cggtcaagtg gcgtggatgt ataaaggaca cattgccacc gatccacgaa ctggtgaaaa
     1381 aataaatact acgagaagag gttttagtag caaacaagaa gctcaaaaag attatgacga
     1441 gtacagacat caaatattat acggtgttaa aaagaaagca cctgatatga catttgaaga
     1501 cctgtataat gaatggataa ctcatcagcg taccagtgtt aaagcttcaa caatcgcaat
     1561 ctcagtaaga tacgcaaata atcaaatact tccagcattt ggtaagctta aactatcgaa
     1621 tatctctgtc ccatattgtc aaaaagtggt agatgaatgg cacagtaaat atgaaagtta
     1681 cgattatatg agaaaacaga ctgcacaaat tttacgctac ggggttgcaa tgcaatatat
     1741 tgacaataat cctatggaaa aaacacttct tccacgaaaa aaagagtatg agaaaaatcg
     1801 taaattttac tcaaaagagg agcttaataa tttactggat gcttttaaag attttggtaa
     1861 catgaaacaa tacgccttct tcagattatt ggcatacaca ggtatgagaa agagtgaagt
     1921 actcgcttta cagtggaaag acatagacac gtttaataaa gaactacatg tcaataaaac
     1981 tcttgctgta gatgaatttg gtaaagttat aattcaatct ccgaaaacaa gagcttctag
     2041 acgagttatc tctttagata ctgagactct ttctatcctc aacaactgga aattacagca
     2101 aaaagaagag tatttaaaac ttggatacaa tactagctct aaagagcaac atgtttttac
     2161 aacagttaaa aacactctat acattccaaa cactgttaat gattggttaa gatatatttt
     2221 gaaaaaatat aacttgcctc gtattacacc tcatgggttt agacatacac atgctagttt
     2281 attattagaa gcaggagaat ctgtcaaggt agttcaacaa cgattagggc atgaaaatag
     2341 taaagtgaca ttagacatct atgcacatat tacaaataat gcacctaaga aaacaggtca
     2401 agactttgct gatatgatgg ctcatcagta aaagagctgg tcaaaatctg gtcatttcta
     2461 gtatacaggc aaaacaaaaa agctagaacc cttatatatc aagggttcta gctcctataa
     2521 caggtttacc caacactgcc ttccaatgac tcttttggtt ctataatatt ttgtgttggt
     2581 ggaaaatcga aagcgatttt ctactaacac ttttttcttc gtttcaactc aaaaagagac
     2641 tcacttttgg taaaatttag tcgaccaaaa cgtcacccta ccaaaggagt ctctcaatgg
     2701 ataatcatac tagaaaatta ctcggattaa cagataaaca tttatttttt gatgaagaat
     2761 ggctgattga aaaagaatat aaaggcgttc aagcgcagtt tattaaaggg agattggatg
     2821 actcaccttc ccattgtgaa cactgtggat cgattcgtat tatccgcaac ggttcctata
     2881 cgacacgcac tcagatgttg aaagtcaaag aaaagttgac tattttagaa ttaaaacgta
     2941 cccgttttct ctgccatgat tgtggacgga ccttttctgc gaaaacggac ttagttgatg
     3001 aacatcatca actaacaaag gaactgaagc aggcgattct catggaactg tacgaaaatc
     3061 aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg acacggatct
     3121 tgcgtgaagc aacgaagcat taccagccac gtatgaactt ccttccgacg gtgttgtgta
     3181 tggatgagtt caaatcaatg aagtctgttt ctggttccat gagttttatt tgtgttgatg
     3241 ggacaaccaa tcagctattt acgatccttg aagatcgcag actttacaaa ctaactcaat
     3301 acttcctgcg ctttcctaga aa
//
LOCUS       DAYYGS010000160         3265 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000160 DAYYGS010000000
VERSION     DAYYGS010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.168"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..701
                     /locus_tag="ACHA44_002779"
     CDS             <1..701
                     /locus_tag="ACHA44_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFM8905996.1"
                     /translation="FKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVNS
                     AENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTIK
                     GIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTPS
                     VQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQM
                     KRDAGAVGGTLYAT"
     gene            691..1431
                     /locus_tag="ACHA44_002780"
     CDS             691..1431
                     /locus_tag="ACHA44_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFM8905997.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            1428..>3265
                     /locus_tag="ACHA44_002781"
     CDS             1428..>3265
                     /locus_tag="ACHA44_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HFM8905998.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCEIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWR"
BASE COUNT         1228 a          396 c          630 g         1011 t
ORIGIN      
        1 ttttcaaaga tacttggaaa aatattaaag atacggcata caatacgtgg atttctataa
       61 aaaataccat gattaataca tggaataaca ttaaggactc tttctggaat attgttactg
      121 gaattgttaa ttctgctgaa aatgcatgga caaatctaaa aaatggtgtt tcaaatgcta
      181 ttaatcgggt aaaagaaatc tttaattcgt taagggaagt caatttattt gaaattggca
      241 agaacatcat tgatggactt ataaatggtg taaaagaaaa gtggaatgct ttgaaagata
      301 ctatcaaagg tattgcaggt aatatcaaaa attggattaa aggagcttta gatattcatt
      361 ctccatcaag atggatgcgt gatatggttg gtaaaaatat tgttcaaggt attatcgttg
      421 gtattgataa agagcaaagt aagttagatc aaaccatgac aaatttagta aaaacaccgt
      481 ctgttcaacc tgtagtaaat ggaataagtc acctaccaat tacacaaaca aagcaaaata
      541 tatctgctag tgaaacaaaa gaaattcatt tacatttaaa tgtttatgga gatttacctg
      601 attcaatggt tcgaaagatt actacaaaaa ttaaaacaga attgacaaga caaatgaaac
      661 gagatgctgg tgcagtagga ggaacattat atgcaactta agagaggaca attttttatt
      721 aatcaacatt attcttctga atttaatgtg tatattcaaa acaggcctgc ttctgtttca
      781 gctagtcgag taattgaatt gagagagcga gaaggaaatg attctatcat tattgataaa
      841 ggctattaca aaaatgtgac tagaaagatt gagtgttact ataaagcacc ttcaattgat
      901 atggtgcagg agtgggaaga tcgaatcact gaatggttag atatgtcgtc ttatagtgat
      961 tttattcttt actatgatga acaatatatt tatcaagctg tagtaataga aggacctgaa
     1021 tttaaaggaa caagaaaaac aggaaatatt gtcccttttg aatttacagt gagtattcga
     1081 ccatttaagg aaaattacaa tggtagattt actattcgac aaacagaaac ttttgaaatt
     1141 tataacccag agaagtattc ttcaaaaccg cttattaaat tgagtggttc tggagatgct
     1201 tctttttata ttaataagga taaatatgat ttgaaatcgt tggacagaga attatttata
     1261 gattctaaat tagaagaggc ttatcgaaaa ttagatggta atttagaaca tcaggatcag
     1321 gtcaccttat ttttagattt tccattttta tatcctggaa aaaatgaaat taaatggacg
     1381 aaaaatattc attcatttga aataatgcct aggtggtgga gaaaagtatg aaaccaagaa
     1441 tatatagtcc tactgaaaca gatttctcaa cgaatggctt aggaatttta aaggattgta
     1501 caagatgtga aatatatgaa gtagctaatg gaaaatatga attagagttg gattatcctt
     1561 taggaactag atttgatgaa tattttgaaa atgactatca aataaaagca aagccaaacg
     1621 atcaagaaga gtatcatatc ttttttattg atgataaaga tatagatact tttttaaata
     1681 cagtaactat ttatgctcag agtcgtacaa atcgacttgg aagacgggta gtcactcttg
     1741 tggaagtgga ctctaaaact ggtcaagaag cgatatcaat tattgaaact aaaatggata
     1801 aaaaatctga catacgactt tattctgata ttacggctgt ttctagcaca atctttgaag
     1861 cgagaaatgt tttaaattgt attgctggtg aacaaggatc attgcttcaa tattggggcg
     1921 gagaaattaa acgtgaacca tttaagcttt ctttgttaaa gcgaagaggt cgcaataatg
     1981 ttggaacgat tcggtatgga aaagatatgt ctggtttgaa ggtcaaatta gattggacag
     2041 gtataaaaac aagaattatt ccatatgctg atcctcaaag tgaagtaggt acgactaatc
     2101 gaatttatgg ttcgccagta gatagtgcct atattaataa ctatcctgat gtctatacag
     2161 aacatgttca gtttacagaa gaacaaggag taaaagacat taagagctta aataaaatag
     2221 ctaaaaatta ctttaaaacg atcaatcctg gctgtgataa gcctaaagtt tctattacgg
     2281 ttgaatttga taagttgact gatagtgaag aagcgaaaga atttgcgaag attagaaatt
     2341 atggtctgtt tgatacattt aaaatatatc ataaaaaata taatatttat cttgaatcaa
     2401 aggttagtgg actacaatac gattctttat ctgaaaaaac tttgaaatta gaagcaggag
     2461 acattcaagt tgctttttat cagcaacaag ctgtaactat tcaggataaa ttaaaagatt
     2521 atgcaacgaa taattatatg agtgatttta atgattatgt ttcttcaatg attacaggtc
     2581 aaggcagtgc aggaggatat gtagttttat ggccaaaaga aaaaccttcc aacatcttta
     2641 ttatggataa tccagattta gaaaaggcaa gagaagtact aagaatgaat aaaaatggga
     2701 ttgctttttc taagaaagga tggcgggggc cttttaattc agcttggaca ttagatagta
     2761 tatttaatgc caattttata caaactggat taatcaaagc agatatcttt caaaattctt
     2821 ttaacaagac tggagatgta ttgaaattag ttaatggact actccaaatt tggaacaaca
     2881 aaaagaaaat aatggagtta actaaaaaag gaatggaatt ttggaattct aagggttcaa
     2941 ttggaacgat tggaacaact gattctgttg gtaatccttt tcctggggct tctactccaa
     3001 cccctattga agataattct ttagttattc gtacaaatgg agacggcaaa tatattttga
     3061 tttctcctaa agtaggaaaa ggattagttt tattaggaaa tggtaaagca atttattttg
     3121 gagacttaga tgtacaagga aaacttacag ttaaaggaaa agaaataatt cctggacaag
     3181 gtggtggtcc atcgggaggt ggagaatcta ctggtgggta tcctagtgaa ttaaaaacag
     3241 atgcagaaaa gagagcttgg agaat
//
LOCUS       DAYYGS010000161         3247 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000161 DAYYGS010000000
VERSION     DAYYGS010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.169"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1701
                     /locus_tag="ACHA44_002782"
     CDS             <1..1701
                     /locus_tag="ACHA44_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8905999.1"
                     /translation="NNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFH
                     LSRIKGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIW
                     LLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPA
                     VLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKND
                     IKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQAL
                     NNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTP
                     ISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENY
                     AKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKG
                     KYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGE
                     NKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK
                     "
     gene            1688..>3247
                     /locus_tag="ACHA44_002783"
     CDS             1688..>3247
                     /locus_tag="ACHA44_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HFM8906000.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVT"
BASE COUNT         1108 a          478 c          542 g         1119 t
ORIGIN      
        1 aacaacaaat ctatgaaaat aagtaaaaaa gcatttcttt tagtagttct tatcttactt
       61 agtactttat attcagtaaa ttttatgaga aatgctcaag aaatttatac aacaggtgat
      121 ttatcttttc atctttcacg aatcaaaggg ttatcttcta tttttgaagg gccaatcaac
      181 tatacaacat ttaacaatta tggagatgga ctgaactatt tttatccttt tcttacaata
      241 attcctgcag tcgtttttta tggaatttca aataacttaa tattaagtta tgttctatat
      301 atttggttat taaatatttg tacgattctg attagttttt attatggaga aaagtttttt
      361 aaaaagatag atgctgcttt tttatttagt tgtttatata cttttgcaac ttatcgaaca
      421 gttgatatct attatcgatc cgcaattgca gaagcaatcg ctcttacgat tatcccagct
      481 gttttatatt atgcttatgg attgatattc aatagagaaa aaaaagtcat cgctttatct
      541 gtcagtataa gtctacttgt gtatactcat gtcttaacta cactattgtg tagtgcattg
      601 attgcttgta tgattatagt tcgtttgttt ttaagcaagc aaataaaaaa cgatataaaa
      661 tttgtggtcg tgaatttagt aaaggctgtt tttttgactt gtgcattaac tagctatttt
      721 tggtatccta tgttgcagca gatgaattat caagaaatac acagtccctt caaaacagat
      781 ttgcagctag aagcactttc tatttcagat agtatcattc aagcgttaaa taacgatatt
      841 acaacataca ctatggggtt attaggattg gttgcgttga tttttccttt attcatactg
      901 aaagaaatgg agaaaaaaga aaaaatcatc tatgttggcg tagtttctac atgggtatgc
      961 gtaactaatt tatttccatg gtttttgttt caaaaaacac cgataagctt actccagttt
     1021 ccttggcgta tcttaggtat ccaaatcttt ttttcttcaa tgatcataac gcttgttttt
     1081 atgaaaaaga ctatgaataa aaaatattca tggttaatca ttggcgctac tgtggtgttt
     1141 ctactcgtca cgtctatagc agcaaaagaa aattacgcaa aagttatcca atcaaaacac
     1201 ggtcatattg ttatcaatga agaaacactc ccacgttata caacatcaga tatgggagga
     1261 ttatacgatt acgccccaac agaagcgata aaagaaaggc ctcacttaga aaaacatgaa
     1321 gtaaaagtag gagaatcgtg ggaaaaaggg aagtataaag ctgcagataa taacattact
     1381 tacacagtgg caagcaacaa aaagcaaaaa gtgacagtac cagtatatgc ttatttaggt
     1441 acacaggtaa aagtaaacgg taaagtcgta aatgactctt ataaaaaaca tggcttagtg
     1501 aatgtggatg taaatgctgg agaaaataag atagaaatta caacaaaata tactccaact
     1561 gctctcatgg ctttatggat cagtattctg acttattgtt tagtggtgta ttcaagtttt
     1621 aaacgatttg cacctaataa gttgaaagat tctcgatatg tgactaaaaa ggagataaac
     1681 aagaagaatg aaaataaata aaaaagtagt aatattgagt ggattaatta tagttagtag
     1741 tatatattct ttcatctttt tcaaaaatac acagagtatt tataccactg gagatctttc
     1801 ttttcactta tctcgaatca aggggttatc gactattttt accagtccga ttaattatga
     1861 gacattcaat tatactggat atggtgtaaa ttatttttat ccctttctga ctttttttcc
     1921 agctgtaatg ttatattgga tgacaaaaaa tttgattgta agctatatta tttacgtttg
     1981 gttattgaac ttgtgtacaa cactcgtagc ttatcattat ggcgagcggt ttttaaaaca
     2041 aaaaaaagcg gcatttttat ttagttgtct ctatattttc tctgcttatc ggacagtaga
     2101 catctactat cgttccgcaa ttgccgaagc tatcgcaata acacttgtta tccctgtatt
     2161 attttatgct tatcaaatca tctctggaaa agaagaaaag tatccgtctg taaagctagc
     2221 attgagtatg agtttgctag tgtacagcca tgtactatca acattaatga gtacagcatt
     2281 aattatcatt tttattttta ttcgtttggt ttctaaaggt ttcaagaatg cagattttat
     2341 tgctatattt aaaaaattat ttagtgcggc aggaatgaca ttggttctga cgagtgcttt
     2401 ttggtatccg atgtttgaac aaatgctcta ccaaaaaatc aacaaaccta gcgtgacaaa
     2461 tttatatgct catgcatcta atgtgttcga tagtctgact gaagctatga acaacgactt
     2521 aactacttac tcaatgggac tagttggtct gttatcttta tgtattcccc tgattttatt
     2581 taaaaaatta acaaacatag aaaaaaagat ttattatggt acgtgtttga cttggctagc
     2641 tacaactagt ttggtgccat ggtatcttct gcaaaatacg ccagccaaat tgttacaatt
     2701 tccttggcgg atcttaagct tgcaaataat attctcttct ctgatattaa cgatgatatt
     2761 tttcaaaaat agaagataca ataagacaag agaactattt tatttattta tgactgtttt
     2821 gttattaatc actttaactg tttctagtaa aaaaaatttc aatcaaaaaa taatttctca
     2881 gccgggacat attgttgtaa acaaagaaac agtagaggcg tataccacta aaggaatgga
     2941 ttatttttta tatgattatt ctcctatcgc ttcattggag aataaggagc atataataaa
     3001 acacgaagca aagattggca gtcaatgggt aaaccttcct tataaaacaa ctggtagttc
     3061 tatcatatat agagtaacta atgagaagag ccaagtaatc acacttcctg tatatgctta
     3121 tttaggaacg aatgtagaaa taaatcatca acctgtaaag gagcaggtga atgctaatgg
     3181 attggtacaa ataagcggag tcaaaggagt tgctgatatt aaagtaagca caagttatac
     3241 agtaacg
//
LOCUS       DAYYGS010000162         3158 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000162 DAYYGS010000000
VERSION     DAYYGS010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.170"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..592
                     /locus_tag="ACHA44_002784"
     CDS             <1..592
                     /locus_tag="ACHA44_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8906001.1"
                     /translation="YVFPDYYNDAQGIVLMNFINPILVLIVVSPLTLFLSKKYGKKEL
                     ASVGMFFSAIVYLILFLIKASNMYVFLILSSVGYMGLGVFNTVIWANITDVIDDQEVK
                     SDQREDGTVYAVYSFARKIGQALAGGVGGWTLSIIGYDSLANVQTGDVLEKLYNASTL
                     IPSICFLIVGLILFFLYPLSKDKVLENCELLKQKHK"
     gene            602..1396
                     /locus_tag="ACHA44_002785"
     CDS             602..1396
                     /locus_tag="ACHA44_002785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFM8906002.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            1409..>3158
                     /locus_tag="ACHA44_002786"
     CDS             1409..>3158
                     /locus_tag="ACHA44_002786"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HFM8906003.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGL"
BASE COUNT         1135 a          373 c          620 g         1030 t
ORIGIN      
        1 ttatgtattt cctgattact ataatgatgc acagggaata gttttaatga attttatcaa
       61 tcctattttg gttttaatag ttgtttcgcc tttaactttg tttttaagta aaaaatatgg
      121 aaaaaaagaa ctggcatcag taggaatgtt cttttcagca atagtatatt tgatcttatt
      181 tttgataaaa gctagtaata tgtatgtatt cttaattctt agttcggttg gctatatggg
      241 tttgggtgtg tttaatactg ttatttgggc aaatattact gatgttattg atgatcaaga
      301 agtaaagtct gatcaaagag aagatgggac cgtatatgcg gtatattctt ttgctagaaa
      361 aataggtcaa gctctagctg gaggcgtcgg aggttggact ctttctatta taggctatga
      421 ctctttagca aatgtacaaa cgggagatgt tttagaaaag ttatataacg cttcaacatt
      481 aattccatca atttgttttc tgattgtagg attaatttta tttttccttt atccattatc
      541 taaagataaa gtcttagaaa attgcgagct tttaaaacaa aaacataaat aggagagaaa
      601 tatgaataat tggatatttt gtattgattc agatggctgt gttatggata cgatgactta
      661 taaacacaat ctctttttcg gaccaattgc tgcagagatt tttgatgttc atcatagaga
      721 aaaattccta caggattgga ataatattaa tttatattca gagactcgtg gaataaacag
      781 atttttaggt ttagttaaaa cattagaaaa aaatgatgta acatcgatta atcgattaaa
      841 gcactgggta tctctaacaa aagaattgtc taatccatct ttgaagaggg aaatagaata
      901 ttctcagtca gaggatttat taaaagcttt aaaatggtcg gaagaagtca atcaacagat
      961 tagtcaagca aaaggattag ataaaccctt tttaagtgct agagatacta taaaagctct
     1021 gaaaaaatat gggaagataa ttattgtaag ctcagctaat aaggaagctg ttcaagaaga
     1081 atgggaaaga catgaattat tatcattagt tgatgaactg tgttgtcaag ataaaggaaa
     1141 aaaagaagat attattcgaa gtgtaattga aaatggttgt gatttggata agatattaat
     1201 gattggtgat tctcctggtg atctagaagc tgcaaataaa aataaagttt tcttttatcc
     1261 tattttggtc aataaagaaa aagaatcatg ggaaaactta agaactgatg ttttatttaa
     1321 atgtttatcg ggagattatg tagatattga acaaaaatat atagatactt tttggaaaaa
     1381 tcttactaat aagtagagga gaaagaaaat gactataaaa gtagatttac gaaaaaaacc
     1441 atataattta gatgaagatg ctatttcttg gattgaagag acgattgatt ctatgacttt
     1501 ggaagaaaaa ataggtcaat tgtttgttaa tatgggatct agtcgtgata aaaattactt
     1561 gagtaatatg gtcaatcagt accatattgg agctgtacga tataatccag gaactgctga
     1621 tgaggtatat gatcaaaact atatcttaca aacagaaagt aaaattcctc tattaattgc
     1681 tgctaatacg gaagcaggtg gtaacggagc ttgtacagat ggtactgaag ttggtgtaga
     1741 agtaaaaatt ggtgcaacaa atgatgcgaa atatgcttat gaattaggga gagtttctgg
     1801 gattgaagca tcggctattg gatgtaactg gagttttgct cctattgtag atattaatag
     1861 aaattggaga aatcctatca tttctagtcg atcttttgga caaaaccctg ataaagtttt
     1921 agaacttagt ttagcttata tgaaaggtat tcaggagagt ggaattttgc ctgcagctaa
     1981 acatttccct ggtgacggta ttgacgaacg agatcagcat ctatctttta gtattaatag
     2041 tttaggtacg gaagaatggg ataattcttt tggaaaagta tactcaggct taatagaagc
     2101 tggattacct tcaattatgg caggtcatat ccatatgcca gcttattcaa aacattttaa
     2161 tcctgatctg aaagatgaag atttacttcc tgcaacttta tcaaaagaat taatcactga
     2221 cttacttaga ggaaaactcg gatttaatgg attagtagtt actgatgcta gtcatatgtt
     2281 aggaatgact ggggcaatga aacgatcaga attattgccc acttctattg cagctggatg
     2341 tgatctattt ctatttttta atgatccaga tgaagacttt aattatatga tggaaggcta
     2401 tcgtaaagga attatcactg atgatcggtt attagatgcc ttaaaaagaa ttttagggtt
     2461 aaaagctaaa ttgggattac acaaaaaagc aaaagaacaa attttattac ctaagcaaga
     2521 agctctggaa aaaattggtt taattgaaaa taaatcaatt tttaaagagg tggctgacca
     2581 agcaattaca ttagttaaag ataaacaaga tatttggcct attgtaccag aaaaatataa
     2641 aaggatttta ctagtagatg tacatggggt aaaaggtggg tttggtgaag tagttgctgg
     2701 aaatagacct caagccattg atttattgaa acagattttg tcagcaaaag gatttgaagt
     2761 tgaaaaatac atttcacctg aagaagaatt gaaaggatta tcgcaagaag aaaaaattaa
     2821 aaatatagct ggaatatatg ctgctaagag accaattaaa aatttaacta aaaaatatga
     2881 cttgatcatt aatatagcta atgttaatcc aggcacagtt caacgaattg tctggccagc
     2941 aagtaaagga actccggata taccgtttta cattcatgaa attccaacaa tttttgtatc
     3001 tgtacagtgt ccgtttcatt tggctgatgt acctcaagta aagacttata ttaatactta
     3061 tgatggaaaa caagaaactt tagaaatatt ggtggacaaa ctaatgggtc tttctgaatt
     3121 taagggagta agtccaatag atgcttattg tggtttaa
//
LOCUS       DAYYGS010000163         3111 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000163 DAYYGS010000000
VERSION     DAYYGS010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.171"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            294..2354
                     /locus_tag="ACHA44_002787"
     CDS             294..2354
                     /locus_tag="ACHA44_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HFM8906004.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2437..3006
                     /locus_tag="ACHA44_002788"
     CDS             2437..3006
                     /locus_tag="ACHA44_002788"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFM8906005.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKSDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
BASE COUNT         1126 a          549 c          584 g          852 t
ORIGIN      
        1 ctgaaaaaat cagctagtat ttatggatgg cagctttgtc tcacatattt attagctgat
       61 ttatgttttt ttcaaagaaa aggtaactag atataactag atataactag atatattact
      121 taacaaaatt aaaaagtaca taagttatta ttccgatttt cttgaaacaa taaaaaatca
      181 aaaaaaatac tctcctaata gtccatcttc ttttgatttt atttatataa tgaaatcatc
      241 ttatgaaaat tactacttca tgtttagaca agagtaggga ggactaagaa taaatgtgtt
      301 caaaaagacg ttcaaaacaa ctaaataaac acgcttgtcg tttagataag gaacggtcgt
      361 gttctgaaaa acactacgtt aagtttatga tgcgcacgat gctgatcagt ttatctgtag
      421 gtgtctatat gcatgtaccc gttcaggcaa cgacacaaac catcaaggtc gtacgtacct
      481 ctgtttctga gaccactgat tcccctatca ctattactca tactttcact gatgcaaatg
      541 gaaaacagct acttcctgca ttgacttcct ataataaagt cagttatcct ccaacgataa
      601 accactactg gttaattcct aacaaaacat tatggacatc ggaaaccgga agcagtgtgc
      661 cgctttctat cttttccgat atgactgatc ctacaaaagg tgatcagtta aaacagatca
      721 ctgatatttt gaatcatgct ttgacagata taaaggacgg tggaagcgca caagtttctt
      781 atgtatatga aaaagattat tcacagtttg aaagaaagaa aatcgatgtt ctggcaggga
      841 caaaagtttc gatatctgat ctggtagaaa ggctgaagaa cgcagatgga acacaaggag
      901 atatgtccaa agtaatttca aacaatggga atatcgtgga tacgactaat acaggaacat
      961 ctactatttc cttgtcctat tttgatccaa ttgctttaaa aacaatgact gcatctgctg
     1021 tgatccatgt tcgtgctgat caaacagcta tcaaaggaaa agatcttcac ttaagaacag
     1081 gagagagtta cgcccctaaa gatcttgttg aatatgtaac aaaaagcgat ggaacttcag
     1141 gaaataaaga agaaatcaaa atgaacggga cagtagatac tacaactccg ggagaatatc
     1201 ccgttgtatt aacttatata gactcgaaaa catcaaatgt ggcagaaaca acagctgtag
     1261 tttatgtaga agcatctgct gcttcatcga ctgtcatcac tcattttata gatagtcaaa
     1321 caaataaaga acttcaagca gaaacacata tgagtagtgt aaatcgtatt cctgatttgc
     1381 gcctacctgc tgaattttct gatcgagtgc caaatataga tttatcgact tatgaacaaa
     1441 atgggaaagt cacttctatt tctgattgga tgcaagcaaa cggattagac agaaaaagtc
     1501 actggtcgga gatccttgaa gtacttcact cagaactgat gaataatagg aagcaatcag
     1561 gaggggaaat tacctttact tatgtttata taccagacaa tcgttcatta gaaggtcatg
     1621 atcttattgt tcatccaaac gaaaaaataa acaaagaaga gttgatccaa tcagcaacaa
     1681 attccttagg taaacccgta ccgttaaagg atgtatcggt tacaatcaat gaagtaccat
     1741 tggaggatgg ttatttgacc aaacaaacag gtgattttac gattatgtat agttatcatg
     1801 acccgtattc actccaagaa ctccatgctc agtcactatt acatgtttta ccattaaaca
     1861 aacagataga tcaaaaagaa caaaaaagac aagaagaaag cttgtcagaa aaaaaacaac
     1921 aaatggctca tgcagtttct ccaacaggga aagtaaaaca aacaagaagc ctagagttag
     1981 caagaatatc tagaaaaatc tcagaatcta ataaaacgat aaggcaagaa gctcatttgg
     2041 ccccacaagt ttctggattg gaaaatgaaa ttttagagcc taaaataaaa caacaatcag
     2101 aaaaagaatt tgttgctaaa cagtcggaag atcagaagga atcagatgac cagaaggaaa
     2161 cgaagaaaaa acgcttgtca actattgatt ccaccgatga gcatctgcag ccgcaagtac
     2221 cacttcctag tccgggaaca cccgcacctg gtggaggtat tccttcttct gctttaggag
     2281 atttcatgcg aggtatggca ggaacatggg tatacgctaa tcgaaatgaa tttgacgcag
     2341 atgactggtt ctgatttata tctctatctg ttatagtcaa ataagtagct gagtcatatg
     2401 ttatttggca taatggagac gaggagggat agaaaaatgt tagataaaac gttaccttac
     2461 gcagaaatct ggatgacaag accacttcaa caaaatgttc caatcacgcc tttagcggaa
     2521 ggttttgaat ttcgatttta tcaaaaagaa gatgaatctg cttgggcgga gattgagact
     2581 tccgttgggg aatttcaaac gacagatgaa gcacttgagt attttcaaca aaactttgct
     2641 ccttatcctg aagaacttga aagaaggatg ttttttgtag agacaaaaaa aggagaaaaa
     2701 gtagcaacct gtaccgcttg gcgaaaaaaa tcaagagagc aagtgtatcc ggtttttcat
     2761 tggttagcag tgaaatcaga ttaccaagga aaagggctcg caaaagcatt gactgccaga
     2821 gtcttgcaag aatttcctac cctccatacg gacggaccaa tctatcttca cacacagaca
     2881 tggagccatc aggcgattgc tctttataaa aaaatgggtt ttacttttat tgctgaaaat
     2941 ctagacggca cacctaatcc agattatgaa aaagttatgc aggttttaag taaattagat
     3001 aaataacgaa atggtgaaat aagtcctcag tattgggata aaaaacgaaa tagtcattcc
     3061 tcatatctta cttttttgag ttaatactat cggataacta atggcatgcc a
//
LOCUS       DAYYGS010000164         3070 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000164 DAYYGS010000000
VERSION     DAYYGS010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.172"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1980)
                     /locus_tag="ACHA44_002789"
     CDS             complement(<1..1980)
                     /locus_tag="ACHA44_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen binding domain-containing protein"
                     /protein_id="HFM8906006.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKNVINLNENELTYTLHVSMFSGTRNTLVI
                     EDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRFSANNTIGGPTSFTLSFKVNVEAYAVG
                     DQILNFATLVNSDNRLNTSTVRTRKIDGRIKVIKTDENGQRLAGAKFEILSSDSQVIQ
                     EGITAENGEFLSEPLPVGSYQVRETEAPEGYLLDATVHDVFINEYSIEEVTLTIENIL
                     DTGGVELIKHAEGSGEVLQGAVFELQNREGETLQTG"
     gene            complement(2652..2920)
                     /locus_tag="ACHA44_002790"
                     /pseudo
     CDS             complement(2652..2920)
                     /locus_tag="ACHA44_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          840 a          684 c          495 g         1051 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacaaattcc tcggaaacat aatgagttct aacgtttgct tcacctggat tcgttacttc
      781 tgcgatccga accccacctg aaataggttc tctatttcca gtggaactcc atgtagtcgg
      841 attcggtgcc aaagttgttc cttgcggtag tacatcggta accactacac cgtcttttcg
      901 tgatccttca taatttacct gcaaaataaa tttattactg ttggtcacgt cttgcccgtc
      961 aatcagcaga ttcccaagat ttccttggct gtcgatttct ctagagccat agtataattt
     1021 cgaaaaaatg ggtcttgatg aaggaatagg cgtaattgaa aattccgtgt ttgctgctcg
     1081 ctcagcataa gtaagactca cttgaaagtc attatttggc tcagtaccgc ctgattgaaa
     1141 gaaagcaact tcaaacgtga tactgaatgt atacgactga gcggcttcat ttaatccttc
     1201 ataatcaaag gtaaatggaa aaacaacgtc tgtgccttct tggtaaaatg ccagtggctt
     1261 taaaggtgaa tcatcagcga cctccactct aatgaactca attaagccaa tcgatgctct
     1321 aggaactcgt acgaccatct ccccatattc ttcaagaata tcgccagcgc ctccttgaat
     1381 gtatcccttt aacgtttgtg tatgcctgct cggcaaagtg gggcgctcag cagagatgcc
     1441 gatgtcataa ttcaagagct cctcttgtct ttcaatctga gattcctgcg ttatagatgg
     1501 ctcagatgat ctggcttcat taaaagattc acttaagctt tcttctggta tattaaaaga
     1561 ttcacttgtg ctttcttctg gtacattaaa agactcactt gtgctttctt ctggtacact
     1621 gatttctatt gagaccgtat ttgattgaat atcaccgctt cttgcggtca attcgtattt
     1681 acctgaatgc tgggaagaat aagtaatcag acgttcgtaa tcattgagtt gcttcacttc
     1741 cccttcaatc aaatcctcac tcttatcaat gaccaatgca tttagcaggt ctggtgttcc
     1801 tctgactcgc aactggaact gctcacccaa agcagcctcc tcttgatcaa cttcgatcgt
     1861 cagcgaacgc tctggctgtt ctgctaaaga ctcagctaga accgttatgt tcccaaattg
     1921 attgatcaca ataagcataa ctagaaacat acttaacagt ttatgaaatt tgttcctcac
     1981 tattactccc ccttataatt ttgactaaac aacaaggtac ccgataattt tctaaaaaga
     2041 ttccccaact caaaatggct gcgttacctc cctcgtattt tcggttcaac tcaagcgttg
     2101 caatgtaagc tgtaaacaaa gctaatgatt gccgattgtc ttctgttgat tgattcgata
     2161 gctttgtatg acttttagtt gtccgtgtgg cttaaccata attatgattt cacgattcct
     2221 cctgccagtg ccttgcacaa gctggagcgt aaccctaata gttattttgt taatgtactt
     2281 atatactttt atacttttcc attagaaaca atcactaaat cgttgatacc aaggtcagac
     2341 tctagaattt catcagtgag tccctgtatt ggttaacctt catcaatccc aaaaaaatat
     2401 atcaatgctt agcatcaaaa tgaatatata gtttagaata cttgggattc atcgataact
     2461 gatcgcttat ttggccatat tatttggcac ttggttcaac taaacgcatt taggatgttc
     2521 cttcattctc gtcatggatg ttctcaaacc gtcattacta ataaactgtc caccgaatcg
     2581 atggttcatt tcttgcattt tccacgtcca attactcttt ttgctcttta tttgggcgta
     2641 gtttcacttt cttaacttgt atcgtcttcc atcaactcct taatcatctt cttagcttta
     2701 ttggctcggt ttccttgtaa aaggtatcta caaatagtga caatctgtat taaatcttca
     2761 tcattttctg taatgtcaat aatttctata ttctgtacca tatattgcaa agggtttcac
     2821 taagttcata accgaagcga attaaacaac ttttgtatag aataactacc ttttctattt
     2881 ttccattggt aattctatct attaattggt ttaatccttt tgttgtagtt gattccacta
     2941 ccaagatgat tctctcgaat tttaaagctt gaagatgtat tgctattttt gctaactggc
     3001 gtatttgcaa ctatttttcc cttctattct tccttcattg attcccctct aaaattacta
     3061 tccataaaaa
//
LOCUS       DAYYGS010000165         3023 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000165 DAYYGS010000000
VERSION     DAYYGS010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.173"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(240..854)
                     /locus_tag="ACHA44_002791"
     CDS             complement(240..854)
                     /locus_tag="ACHA44_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFM8906007.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1460..1837)
                     /locus_tag="ACHA44_002792"
     CDS             complement(1460..1837)
                     /locus_tag="ACHA44_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906008.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2137..3018)
                     /gene="galU"
                     /locus_tag="ACHA44_002793"
     CDS             complement(2137..3018)
                     /gene="galU"
                     /locus_tag="ACHA44_002793"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFM8906009.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          916 a          632 c          468 g         1007 t
ORIGIN      
        1 tttatttttc tgacactaca tattcttata actcatttct cataactgtg aaacaactta
       61 taaaaataac aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata
      121 actaaaacaa aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa
      181 acgctccatt tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac
      241 tattttccac ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc
      301 aaaaagatcg ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc
      361 aagactgcat catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat
      421 cgcaaaccac tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg
      481 attaatggat gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt
      541 ccttcattcg aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt
      601 gcctgtcctg caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata
      661 cttggtacaa gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg
      721 ctagcatata tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct
      781 tgaagcatca gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa
      841 taacataaac tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc
      901 ctaatctaag gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct
      961 gtttcaaaca caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa
     1021 acaacgtgca gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt
     1081 acaataaatt aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg
     1141 tgtgtgactg gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta
     1201 ttcagctgtt tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa
     1261 atacaaaaaa acattggtat gatttatata tatataaata acataaaaaa aggaatggct
     1321 tatcccgata caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt
     1381 ttttccacca attaaataaa tctctcatct caatagttta tccattattt tatctttttt
     1441 tgcgtatacc tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat
     1501 ctgtactaga aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca
     1561 tatatgggat ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt
     1621 cccaactacc taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc
     1681 agataaaaat cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa
     1741 taagcaatag tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat
     1801 caagcagcga ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat
     1861 aattaaaaaa ttcgcttttt tattctacta tgaatcttct ggcttggctt tcatttctct
     1921 tatgtaatca ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt
     1981 ctgaatctat tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag
     2041 agtcaaaaaa ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa
     2101 atagcctttc attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt
     2161 actcaactgg ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata
     2221 ttcaatattt gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa
     2281 ctctctcgca tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat
     2341 ctcgtttcct gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag
     2401 ataacgtccg ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa
     2461 cccttttact ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt
     2521 agatgtttca ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata
     2581 atccatcatc aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat
     2641 gacgacaaac ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc
     2701 tcttggatga gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc
     2761 gagtaattct gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa
     2821 gtgatcttca atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga
     2881 atccaatgct tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc
     2941 ttttgccatg gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac
     3001 tgcttttttg accttcactt gct
//
LOCUS       DAYYGS010000166         3022 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000166 DAYYGS010000000
VERSION     DAYYGS010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.174"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(287..649)
                     /locus_tag="ACHA44_002794"
     CDS             complement(287..649)
                     /locus_tag="ACHA44_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906010.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="ACHA44_002795"
     CDS             complement(723..1103)
                     /locus_tag="ACHA44_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvfD/Ygs/GSP13 family RNA-binding
                     post-transcriptional regulator"
                     /protein_id="HFM8906011.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="ACHA44_002796"
     CDS             1185..2360
                     /locus_tag="ACHA44_002796"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFM8906012.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="ACHA44_002797"
     CDS             complement(2437..3021)
                     /locus_tag="ACHA44_002797"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFM8906013.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          942 a          578 c          498 g         1004 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca ta
//
LOCUS       DAYYGS010000167         2918 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000167 DAYYGS010000000
VERSION     DAYYGS010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.175"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..962)
                     /locus_tag="ACHA44_002798"
     CDS             complement(<1..962)
                     /locus_tag="ACHA44_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8906014.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHT"
     gene            1134..1268
                     /locus_tag="ACHA44_002799"
     CDS             1134..1268
                     /locus_tag="ACHA44_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906015.1"
                     /translation="MIKVIFKRITELPEFRLFVDYWYVWFFIVALLIGLDVILERKLK
                     "
     gene            complement(1261..1896)
                     /locus_tag="ACHA44_002800"
     CDS             complement(1261..1896)
                     /locus_tag="ACHA44_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906016.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(2009..2644)
                     /locus_tag="ACHA44_002801"
     CDS             complement(2009..2644)
                     /locus_tag="ACHA44_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HFM8906017.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(2678..>2918)
                     /locus_tag="ACHA44_002802"
     CDS             complement(2678..>2918)
                     /locus_tag="ACHA44_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HFM8906018.1"
                     /translation="GGILAATKDENTVMFTKKYWNEMENLKKYIEKQQSILDNNSKET
                     QISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT          831 a          492 c          422 g         1173 t
ORIGIN      
        1 gtatgcctta gtccatgcaa agtaaattgc ttttcaatcc ctaattcttt taattttttg
       61 cgcaaatatt tgctgacggc gtttgatgaa actaatccat tttttaagtt gaaaaaaaca
      121 aaattatgag ggtttttgat ttcaaaattc tcgaataatt ctttttgatt gattttaaac
      181 ttttttaaga gatcaatcgt atgtttatca atggaaattt tcctatttga agtttcgttt
      241 tttgtttttc cccattcatt taatttataa ttccatgttt tattaatagt gatgatttga
      301 tcttcgaaat cgatatcttc ccatgttaat cctagaagtt cagcaaatcg cattccagtg
      361 gcaccagcaa ctaacacgag cataggagaa gagtactgcg cagaaaggtt ttcttctacg
      421 agtttcatta aagatttaaa ttgatcataa tctaaatatt tatcttcttt tggcttcaat
      481 gaatcttttc ctttgattac tgcttttcta gttggatcaa acggaattaa tccttcttct
      541 acggcatctt tcaacgacgc tctaatatgg ttattaaact taataacggt agattttaca
      601 tggtctttag catacttatt taaaaagcgt tgatatccca ctctatctaa atcagaaatc
      661 aataccgctg gcatatattt ctttatgttc attaacgtat cttcgtattt tctataagtg
      721 ataggagata ctgttccttc tttataaagt tgcatccaat cctcaaagta atctgataat
      781 aatagatttt tgcgatcccc tttaaggcct ttgttcaagt tatattctaa ttcgttggcg
      841 gcatcttttg cttctccttt tgtcttaaat cctgattttc ttatcttcgt gtattttccg
      901 tcatcttttt tgtatgaaat ttcgtattgc caactatttc cacgtttgac taatcttgcc
      961 ataattgaat cagctctctt tctctgatac aataggaact ataaagaagc ccattgtttg
     1021 ggttttatta ttttttgcac atcctttttg cttgccggcg caaggatgtg ctatttgtcg
     1081 ttttaatggt ataattggat gtgtattaga tcatccaatc agaggtgttt attatgatta
     1141 aagtgatttt taaaagaata actgaacttc cagagtttag attatttgtt gattattggt
     1201 atgtctggtt ttttattgtt gcattgctga taggtctaga tgtcatatta gaaagaaaac
     1261 ttaagtaaga gttgagttaa taaattgaat tcctaaatga gatattttgt agcccatatt
     1321 ttgtttgccg acatcaccta atactatgga gctaatcgtt ttttctaaca gaccaaaatc
     1381 agataatcta tttaagctac catctattag ctcttgtgaa tagccagaca tgctgatatt
     1441 tccagtgaaa ttttcgtttc cacgactcat taagcatgct aacaattgaa tctcaatcga
     1501 tgttaattga ttcagtacaa gaagataata ctcctcgtta tcccatgaag cgttgttaat
     1561 tcgaagtaaa caattcttat aaagattttt atacatatct attttatttt gtgcctttga
     1621 tttttctacc tcgtcattta tcccttcgaa tattccaatg agttggtcct tgttctctaa
     1681 gttgaaaaca tcttctggaa tttgatcgcg aatgttttct aatgattcgt ttaaattttt
     1741 gtagaatttc tcgatgcgtt tgaaacgctt ctcgttttta gacccaaaat aaagtgactg
     1801 cacaacgcca ccaatataag gaattgctcc aataccgcct tcaactatta attcaagata
     1861 ttcttttttg gttagttttt cattgctatt atccatagtt acctcctatt ttgtcatttc
     1921 ctttacaagc attgtgactc ctgatggcac tcttttgtat aggttttatt attgtgcaca
     1981 atctaactct tagtggggcg tggttcattt attttttact tactttacta ttaatcatat
     2041 cccattgagc ataggatttt tctatgttcg atcctcttga actaatttct cctgtaaaag
     2101 ttactttatc gccctttgta aaggtatcag cattgtttaa gtccttcgca aaaattacat
     2161 atgaaacttt aggtgttgca gaaatatcat tccaactttt atcatcgtac tctttgtctg
     2221 ctataatggc tattctagta gtcattgatt cgataacagt tccttcaaaa gtatatgttt
     2281 taccactaat atatttagta aaagctgtat tttgctcagt agctggagta atagaataat
     2341 aactatctaa gaaagcaccc cagtcatgat tatttgtctc taaaaattct tcaaattgag
     2401 ttaggctttt attgctgctg ttagatgtag tagtttgagt atcagatgat gagctttcct
     2461 gtttgttatt gtcatttgcc tcgtttgatg aggaaagcga gaaacctacg gtagtaaaaa
     2521 taatcccgat aataaggaga atcataggtg ttttcttatt tgtttttttg ataaatctta
     2581 caattaacat gattaaacct ataataaata aaacaatacc agtaaaacca aatataccgt
     2641 ccattaatag atcctctttc tcttttgata ttgaatatta taaaccaagc aattcttttt
     2701 tcttaaaatc gaattcttct tgattaataa tcccttcatc taacaactct ttatactttt
     2761 taatttcatc tgcacttgaa atttgtgttt ctttggaatt attatctaat atagattgtt
     2821 gcttctctat atattttttt aaattttcca tttcattcca atattttttt gtaaacataa
     2881 cggtgttttc atctttcgtg gccgctaata tacctccg
//
LOCUS       DAYYGS010000168         2855 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000168 DAYYGS010000000
VERSION     DAYYGS010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.176"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..600)
                     /locus_tag="ACHA44_002803"
     CDS             complement(<1..600)
                     /locus_tag="ACHA44_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8906019.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSIC"
     gene            complement(720..1367)
                     /locus_tag="ACHA44_002804"
     CDS             complement(720..1367)
                     /locus_tag="ACHA44_002804"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HFM8906020.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(1451..>2855)
                     /gene="uxaC"
                     /locus_tag="ACHA44_002805"
     CDS             complement(1451..>2855)
                     /gene="uxaC"
                     /locus_tag="ACHA44_002805"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFM8906021.1"
                     /translation="LENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQYKNLTQVW
                     LNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPIFEWSHLEL
                     QRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDDPTDDLWYH
                     TELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGLKQALSQRV
                     DHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQFVTGIQLHL
                     MSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVALFDTAEKS
                     GCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMRQQLTTMAQ
                     QSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYLSQIVENIC
                     YRNAYNYFNFFEIGSRK"
BASE COUNT          899 a          602 c          391 g          963 t
ORIGIN      
        1 acatatcgaa aatattcccg caacaattgt aaaagttgat ccaccacgaa ctatttgagc
       61 gcctgaatca tctcttgtat aaataaataa tggtgttaat acaccaatga ccaaacttgc
      121 taaagtagct ccaacatttc ggaatgtcga tagcattgtt ctttcttctg taatagaagt
      181 aatagctgat gccattgatc cataaggaat atttatagat gtgtaaaaaa tactccccca
      241 taatatatac gtaacataca tgtatactac cttaacgctc atagaagctg aaatcataga
      301 agtttgatac attaaaaaac ttgctaaagc gactggacca ctcattctta aaatccacgg
      361 tttaaactta ccctgcttag ttgctttcga tctatcaaca atacgcccca tagttacatc
      421 agtaactgca tcgataaatc ttgctactaa aaataatgtt ccaacggcag ctccgcttat
      481 ccccaatact tttgtataaa agatcattaa aaaagaacta gcaaaaatga aagtaaaatc
      541 gtttccaaaa tctccaaaca tatatcctat tttgtctctt ataccaaatt ttctttccat
      601 acaattctct cccttctgtt gttaagcgtt ttcattctga tgaaagtacg tactatactc
      661 tgaagcgtaa agagtcagta tttatttttt acatttcaga gtattatttg ttttttctcc
      721 taataattct ttatctcata aaacttatct atatatttac tagctactcg acttacttct
      781 tcaaaatcac ctttttcaac agaagccaat aaattcccac ctacaccaac tgcaacaact
      841 cctacttcaa accaatcttc taagttctct atacttactc cgccagtagg cattatattt
      901 acgtgtggta atggagcttg ataggctttt acaatggatg gaccaaaagc agaacctggg
      961 aatagtttaa tgatatctac gccagcagta agtgcctcct ttatctcagt aattgtcata
     1021 caacctggta aataaggaat ttgatataaa ttacaaatct cagctgtttg tcgatcaaaa
     1081 gatggactaa cgatatattc tgccccagcc ataattgcga tacgagcagt aaacgcatct
     1141 aaaaccgtac cggctccgat tattacatct ttttgatcag catatttttt agcaatttct
     1201 ttgattattt cttcagcatt aggaactgta aaagttagtt caatcccttt aattccaccc
     1261 gcaataattg aatttattgc tttaatggcc tcatccttat ttttcccacg tacaaccgca
     1321 atgactccag ccgcttctaa cttacttaaa atttctactt ttttcatatc tatctccttc
     1381 ctttatggat acatactttg tctagctgta ctaacttaca aagaataaat ttaagctttt
     1441 atttcaagta ttattttcta cttccgattt caaaaaaatt aaaataatta tacgcattac
     1501 gataacaaat attctccact atctgactaa gatacatttc atcatctgga attcggcctg
     1561 ctttaaccca ctcgcttagt aattgacata atactcgacg aaaatactca tggcgaggat
     1621 agcttagaaa acttcgagaa tcagtcaaca tacctgtaaa attcgacaac aaactttgtt
     1681 gtgccatcgt agtcaactgt tgacgcattc cttcagctgt atcattgaac caccaagcac
     1741 atcccaattg gattttttga atcatacccc cttggaagct ttgcattgct gttgctagtg
     1801 gaagccaatc agttggattt aatgaatata aaattgtttt tggtaagcac ccactctttt
     1861 cagcggtatc gaacaatgct acaacatttt gtgtcaaact agcttgatcc cccatcgaat
     1921 caccaccgac atctactcca atttgttcct ttaatatact tgaattattt cttaaagcat
     1981 ttaaatgcat ttgcatcgtc caaccatatc ttgtatactc agacatcaaa tgtaattgaa
     2041 tacctgtaac aaattgtgca atttctgata aatggaaatc cgtttttcct gctaaagctt
     2101 ttattaacag attgtcgagt attttttcat ctgctatttc ataaaaataa gtattcaaac
     2161 cgtgatctgc taatcgacag ccaatttgat gaaaatgatc cactcgttgg ctcaatgctt
     2221 gcttcaaccc attaaagtct ttgattggta tagccgctgt ttgactaagt tttaaaatat
     2281 agttcgaaaa gcctgatgat ttgatactcc agaccttatc tggacggaaa gtaggtaata
     2341 ccttgaaaga attatcctgt ttagaaagct cagtatgata ccacaagtca tctgtaggat
     2401 catccgttgt acagatggct ttcacatcca tcatctccaa caattctttc gggcgatagg
     2461 aatccgtttg aattttttcg ttagcacgtc gccaaatttc tggtgcattc ttctcagaaa
     2521 ttgtcaaatc aatatcaaag taacgttgta attccaaatg agaccattca aaaataggat
     2581 tacctggcgc cttctccact gtttttacaa aagctaaaaa tttttcataa tcatcacctg
     2641 gaccactgat caactcttct tctataccat tagctcgcat caatcgccat ttataatgat
     2701 ctccagctcc tttatcattt aaccatacct gagtcaaatt cttgtattgc ttattttcta
     2761 gcaaatatgc tgggtctaaa tgacaatgat agtcgataat tggttggtgt tttgcaaatt
     2821 tatgaaaaag tcttttagct atttcattct ctaat
//
LOCUS       DAYYGS010000169         2849 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000169 DAYYGS010000000
VERSION     DAYYGS010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2849
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.177"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            523..>2849
                     /gene="esp"
                     /locus_tag="ACHA44_002806"
     CDS             523..>2849
                     /gene="esp"
                     /locus_tag="ACHA44_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HFM8906022.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1031 a          437 c          559 g          822 t
ORIGIN      
        1 ttttgtttcg ctaaaaacga gtgaaaaaat gttgataact ccaatcattc gtttaagatt
       61 ctaggaatca ttcagaaatg ctgtgaaacc gatactttat aattataaag aaggtgattt
      121 caaattccga agaaggaatt tgaagtcacc tttttactta tgtcacagtc tcattcagta
      181 atgcgggata aggctcctta ttccgttatt tgaatactct gaagtttaat ctaagaaagg
      241 gtaatatttt gaaaacatgc ttcatcgcat agaaatgaac ttaatttcag aaagaataca
      301 tacatatctt tttatatgtt ttcagctgct gaatggttaa aaagcacaaa tatccgaaaa
      361 aaacatgaag aaatgtactt tttttgttgt aatgttcatg aaattctcta agtaaattag
      421 ttaacataag gttagtaaga ttcctattct gtgaataata cgtatcagat ttatctttta
      481 ttcttactag gaaagtaatt attaagggag ttgatttgaa taatggttag caagaataat
      541 aagagagtat ttctagaaaa aacaaagaaa cgagtattga agtacagtat caaaaaatta
      601 agtgttgggg ttgcatctgt tttgattggt gttggtcttg tcttgggaac aactgaacta
      661 gttaaagcac aagatgaaat ttctccaagc agcccacttg aaacagcgat aagctccgta
      721 caagtaggtg acaaagtagc ttcggggaat acttttcagg agaatcctgg ttatacgaaa
      781 aattataact tttcggattt acaatttagt cctcaagaac taactggaga cacactaaaa
      841 ggcaatacaa ttggatttga agtttacgga aaacataata ttgcagcatc aactaaaaac
      901 tgggaaatcc gtcttcaatt agatgaacga ttagctaaat atgtagaaaa aattcaggtc
      961 gatccaaaga aaggaattgg atcttctaga cggacctttg taagaattaa tgattcgctg
     1021 ggtagaccta caaatatttg gaaggtcaat tatattcgtg caagtgatgg attatttgct
     1081 ggggcagaaa caaccgatac gcaaaccgct cctaatgggg taattacgtt tgaaaagagc
     1141 ctagatgaaa tttttaaaga gataggcata gacaatctca agaccgatcg tttaatgtat
     1201 cgaatctact tggtaagtca tcaagacgat gataaaattg taccgggtat tgatagtacg
     1261 ggctactttt taacagattc agatgatttc tataatagct tagatgtatc tgaaaataat
     1321 cctgaccagt ttaaacatgg atcagtaagt gcaaaatatg agaaacctaa tactcaaaca
     1381 aaagatggtt caggatccac aggtgctaat ggagctataa tcttagatca taagttaacg
     1441 aaaaattata atttctcata ttcatcttcc gcaaagggca cgccttggta tgctaattac
     1501 aaaattgatg aacgattagt tccatatgta gcgggtatac agatgcacat ggttcaggct
     1561 gacaaagtaa catatgatgt ttcgtttgaa tccggaacaa aagtagctga tttagcaatc
     1621 gagcgacggg aaggtcatga aaattatggc ataggttcaa taactaataa tgatttaact
     1681 aaacttatcg attttgctaa tgcaagtcca cgtccagtcg ttattcgata tgttttacaa
     1741 ttgactaagc cattagatga aatcttagaa gatatgaaag caacagcaca agttgaagaa
     1801 aataaaccat ttggtgaaga tttcatcttt gattcttggt tgtcggatac gaataaaaaa
     1861 ttaatccaga acacttatgg aacaggttat tattatttgc aagatattga tggtgatgga
     1921 aaccctgacg ataaagaaga gagcggagac acgaatccat atatcgggaa acctgaatta
     1981 gaagaagtat atgatgttga cacaacagtt aaggggaaag tattcatcca cgagttagcg
     2041 ggaacaggtc acaaagccca acttgttgat aaagaaggta ctgtattagc agaaaaaact
     2101 atcgctccaa atgaaaaaga tggggctcca atttcagata ctgtagaatt tgaatttacg
     2161 ggtgtagatt caagtaaatt aatcgcgaaa gatgaattaa aaatccaaat cgtttctcca
     2221 ggttttgata aaccagaaga aggttcaacc gttattaagg aatcaccaaa agcggttgat
     2281 aaacaaaccg tggtagttgg atttaaacca gatgctaaag aatcaattcg gaataataaa
     2341 aacttacctg aagatgcaga gtattcatgg aaaacagagc ctgatacttc taacgttact
     2401 gatagtacga aaggtattgt aactgttaag atcggaaatc gaactttcga cgtggatgta
     2461 gagtttgctg taaaagcttc tcaagctatg gaaaatgatg caacatacgt acctataaca
     2521 acaactccag aaacgacagt tcaaagtgga aaacctacat ttgataaacc agatgttcct
     2581 ttagctaaag atgccttttc aatttcagat gtctataata agtacttcgg taatgcaagt
     2641 gttgatgcaa acactggtat tgttacattc actccagcta aaggtgtagg agaatcggag
     2701 ccgattactg gaacaattcc tattaaaatt gtttaccaag atggttctgt aggcacgacc
     2761 gatttagcag taactgtaag taaagatatt tatgaaaatc caggagaaaa cattcctgca
     2821 ggctaccaca aagtaacctt caccgcagg
//
LOCUS       DAYYGS010000170         2826 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000170 DAYYGS010000000
VERSION     DAYYGS010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.178"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..224
                     /locus_tag="ACHA44_002807"
     CDS             <1..224
                     /locus_tag="ACHA44_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFM8906023.1"
                     /translation="AITNYIHSPIGKSADLVLQTAIEEFLNGGSLAGKISQLYICDLL
                     VHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            454..684
                     /locus_tag="ACHA44_002808"
     CDS             454..684
                     /locus_tag="ACHA44_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HFM8906024.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(907..1005)
                     /locus_tag="ACHA44_002809"
                     /pseudo
     CDS             complement(907..1005)
                     /locus_tag="ACHA44_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(1270..1725)
                     /gene="tnpA"
                     /locus_tag="ACHA44_002810"
     CDS             complement(1270..1725)
                     /gene="tnpA"
                     /locus_tag="ACHA44_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFM8906025.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1968..2826
                     /locus_tag="ACHA44_002811"
                     /pseudo
     CDS             1968..2826
                     /locus_tag="ACHA44_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
BASE COUNT          910 a          510 c          498 g          908 t
ORIGIN      
        1 ttgctataac taactatatt cattcaccta taggaaagtc tgcagactta gttttacaaa
       61 cagccattga agaatttctt aatggtggat ctttagctgg aaaaatatca caactttata
      121 tttgtgatct attagttcat ggttatgaac aaaacaataa tattgattca gttgaattaa
      181 gagaaaaagt tttgcgctct attattgata aaagtattga gtgaactacc cccacttagc
      241 taaacctaac ggtttttaac gattgaagta ggcaattctt gggatgagag catacttgat
      301 agcttatttt ttactaatag cggtggctct tattgaccaa atatccctgt agttcctacg
      361 gttcttgatt ttatctaaag atatttttat cgtggaagct attgatcaac ttgaaagaaa
      421 aacgtgtagc tcattatcac tagtttatcg agtatggatg aagcaatgga tcatcctctc
      481 ttaaataaat ttattaagga tctgatcctt caaatttttt ccatgatcga tgagcaggaa
      541 cctactgaat ctaaacgaca tcgggcacaa ggaattatta agattgctaa gcgaatgttt
      601 tctataaagg gtgttctaag ctatacagtg ttaattcaaa agattcccaa tcatcttgtt
      661 tataagaaca acgttgaaga ttaaaaaagt gtttcaatgc taaaattgca aaatacttca
      721 atgttatgag acaaactgtc tataagaatc aatttccacc catttcatca cttgttaaaa
      781 gtttatgatc cttttgattt ttatattaat ttatctgtat ttaatacaga ttttatttat
      841 tagttcatct gcctattgta ttttattttg ttgccttacc atctgaagta aatatactct
      901 taattgtcaa attaagttag acagatcatc aatacttacg taactcaaaa atacttccaa
      961 aggtgttcga taatccagtg attttctagg aagattattt ctgcgccagc attttctaat
     1021 gtttgaattt gtgttttgta atcttggttg cttgtgctaa ttcttgaata gccgtaaatt
     1081 gccaaaattt catcaccctt tctggacata agtttctgac accagttaaa atcaagtcta
     1141 tcagtaaccc atacacccgt gaggacgggt gtatttttaa tccccctaga aggggtcgac
     1201 actcgctgcc ccttaaaagg ggatttttaa aagaccgcca actgctcatc ttcactcact
     1261 tacttctctt tattttttaa atggttctac gtactctctt ttacttataa tatatcgaat
     1321 gcgatcatca gcttcttgtt cacggatgta tttttcattt gtcttttgat tcaatccgac
     1381 tgtactcaca tagtatctct tcgaccaaaa ggatttgttt ccgtatttat atttgagatt
     1441 tgcgtgctgc tcgtggatta gtaccgcact acgacctttc agatacccca taaaactgga
     1501 tatacatatt tttggtggta ctcgaacaag catgtgaata tgatctagca ttgcatgtgc
     1561 ttctatgatt tccacatctt tcatttcgca caacttccta agtatttttc caagatcttt
     1621 tcgtaattgc ccatagatta cttttcgttg atattttggt gtaaaaataa tatgatactt
     1681 acaattccaa cgagtatatg ctaaactttt atcgtcattt gacatgaaaa aactcctttc
     1741 gatctgatga gcggttggta gccactcact agtatatcga aaggagtttt tatcgctaaa
     1801 gatacttctt ccacgcgtag aaacgcgcgg ttttcttgtt tcgcacgctt tacgcacaaa
     1861 actgactgaa gtcattaaga aaataaaagg gtctaaaact tttaagtttt gtaccgaccc
     1921 ccaaaagtta gaccaaaaaa tctaactttt ggaggtcggt attttttatg gctaagtata
     1981 cttttgaact taaattaaag atcgttcatg attacttgga tggtaaaggc ggaagtgatt
     2041 atctggcaaa gaaatattcc attaaggctc catcacaagt gaagcgctgg atcaatgcct
     2101 atcaagaatt tggtgaagag ggtctagtaa gaaagcgaca aaagaaaaaa tattctgttc
     2161 aatttaagct ggatgcgata gagttatatc taacaagtga gctgtcctat cgtgaagttg
     2221 ccaacacact taacatgaac aaccctaatt tgatcgctag ttggatgcgt caattccgtg
     2281 agggtgggat cgatggactc tcaaaaacga aaggatgtcc acctatcttg tctaagaaaa
     2341 atgaacccaa aaataagaaa aaatctacaa ctaaagcaac gtctaaagaa cgtgaacgca
     2401 tcgaggagtt ggagaaacga gtccgttccc ttcaaattga aaatgccttt ttaaaagaat
     2461 tgaggaagct gcgaaaacag gaagcccaac aacgacgaat gaagcaatcg cacgaatcat
     2521 cgcaagcctc cgaggaccgt ttaaattagt tgagcttcta gaaactttaa agttccctaa
     2581 ggcaatcttt atgtactggc aaaaacgatt ggaccgtaaa aatccagatc aagagattga
     2641 agcggaaatg cttaaagttc gggagaagca caaggattat ggctgtttac ggatgaccaa
     2701 tgaactaaga aaccgcggtt tctctatcaa taaaaagaaa gttcagcgcc tgatcaagaa
     2761 gctaggaata aaagtaacga catacacacg taaatcacgg cgctacaatt cttatcgagg
     2821 acaagt
//
LOCUS       DAYYGS010000171         2690 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000171 DAYYGS010000000
VERSION     DAYYGS010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.179"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1533)
                     /locus_tag="ACHA44_002812"
     CDS             complement(<1..1533)
                     /locus_tag="ACHA44_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFM8906026.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKI"
     gene            complement(1597..1956)
                     /locus_tag="ACHA44_002813"
                     /pseudo
     CDS             complement(1597..1956)
                     /locus_tag="ACHA44_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
     gene            complement(2134..2454)
                     /locus_tag="ACHA44_002814"
                     /pseudo
     CDS             complement(2134..2454)
                     /locus_tag="ACHA44_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          839 a          504 c          362 g          985 t
ORIGIN      
        1 aatttttgtt aaataaatat cccctatttg aataaaacga ttgactaatt gaacttctcg
       61 actacaccaa ctattaatat cattgatgaa ggaatataat ttatcgtccg atgttaattg
      121 gatttgataa atccaagaat tcaaaaagat agacgttatt tgaagataat aatagtcaaa
      181 ttctatttga aaatctctct ctatttcctt aacatactgt ctaaatcgaa tctcttgata
      241 ggtgggatat gttaaagctg aattattttg gatatcaaca ttcttttgtc taaaatatct
      301 atcatatttc tcaaaaagct gctgcttatc agtgtctttc ttccacgttt gataaagata
      361 ctctataagt tcgttgatat taaaaacttc gtctactttt tccaaatcat ccatttgaat
      421 atattttaat tcattctgtc ttttcataac cttcatgccg ttgaaattca cccattgtgg
      481 taaatgatac atctgaatat aaagttcttc caaatcatct aagaaataaa aggcattggc
      541 acaggtcaat tgaatgatat ttttcaaatt gccaacattt tcactaaagc gatagtttaa
      601 taaaaaatct aataattgtt gagatatcag tattttcttt gctagtcttt cttcttctga
      661 tttaaaaaat atcttgatta actgttttct ttcataaaga gaacgactgt ctaatgttgg
      721 aagtgttagc tttacaggta ttcttctcaa caaagttgat aatagtttat cctcaggtgg
      781 ttctgatgtt gcaaatataa tccagcaact tgatgtgtac cagttttcat tatcacccat
      841 ccgatgaaat ttcctcgtat ccataaactg aaaaagtttc tcctgacatt cattgctcaa
      901 tgcatgaatt tcatctaaaa ataagactcc attattagct aattctgcta aaccctttct
      961 tccatcttcg gctcctgtat atgcaccttt tacatgccca aaaaaattgg acaagaaaag
     1021 ttctggatta ttggcatatt cactacaatt aactgaaatg aaagatgcct tctcttctat
     1081 ataattattg gccaacaaat ccttatacac ataatttgct aaatagcttt ttccagttcc
     1141 agtctcgcca tgaagtaaaa tgggtaagcc atttggcgga tagtagattg ctgctttaat
     1201 ttgagcaatt acatttcgca gacttccttg acttccgata accctttcta ttccaaccct
     1261 aaaagagcta tcataccatt tttccgcttc ctctgaagag ctaaaaattt gttgatttgg
     1321 aactttctcc ggtaaagaaa agaagattac tggacgagta ttgatttttc caaattctcc
     1381 atcttttacg aattcattca aatattgaga gacaatatta cgactaacat ttaatttttc
     1441 agatattttc tgtgctgtat tgttccccca tttattttta atctcagagt tctctatgac
     1501 ataattgaca atgcgttctt ttaattcttc catcacagca cctcctcgac actattcgaa
     1561 ttttgccgac agtatttttc cataaatatg acttgctaaa aaggctttgt ttagatcatt
     1621 caattggaac gttgcttgtt ttgcttgatc cgctaagacg ttgaaaagat actctggcga
     1681 aatcgcatac cccaaaatat caacgattgt tgcaatcaga tcttcttttg attcttcatc
     1741 tgaaagtaat tcacgataca acatcgtttg tttgcttact gtgtcatatt cctctaagga
     1801 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
     1861 cagccccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
     1921 cgcactaaat aacttctgat ttaattctgc acccaaaaag atacactcct atctcttatt
     1981 taaagttgct atataccctc ttattttagc atactcagtt ttagaaaaag caaaaaaata
     2041 aagaaactta ttcttgataa caagctacgt gacgtatatc cctaacacaa aaatagccat
     2101 atcctttggt ttggatacgg ctatttgata ttactcattc aattatcaaa tcacagtagg
     2161 tgtgaatctc atcgggatcg gcaatatcta cttctgatta ggatcaaagg tgattttctc
     2221 aataaaggtg tgatacaaag ctttaacttg cttcttctca ctctggataa gaaagcaatc
     2281 caaactagtc aatatctgtt ggacatcttt gacttgaatc ggttcccctt gttgttgaaa
     2341 gatccctaag atttcattct ctcgaatttg taactgtctt cgtttttcag tcaattcatt
     2401 taaacgatct tttaacatcg gaaacaattc gggactatct tccaaagcag cttcctgttt
     2461 ttgctctaat agttagattt tgcgtaccat tttcttcttt tattttcctt cgattgctga
     2521 ttttaatata gtctaaagga aatttatatt tattgtattc tttataactt caagagaact
     2581 tcgtttgagt attactgtat ttttttaatt ccataagaaa attagttatt acctgccacc
     2641 taatcagcaa atctgaacct gagtcaatat aattaatatt attttccatc
//
LOCUS       DAYYGS010000172         2679 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000172 DAYYGS010000000
VERSION     DAYYGS010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.180"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            702..788
                     /locus_tag="ACHA44_002815"
     CDS             702..788
                     /locus_tag="ACHA44_002815"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HFM8906027.1"
                     /translation="MFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1091..1315)
                     /locus_tag="ACHA44_002816"
     CDS             complement(1091..1315)
                     /locus_tag="ACHA44_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906028.1"
                     /translation="MEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNMTPERKRS
                     LEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1681..1965
                     /locus_tag="ACHA44_002817"
     CDS             1681..1965
                     /locus_tag="ACHA44_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906029.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1968..2384
                     /locus_tag="ACHA44_002818"
     CDS             1968..2384
                     /locus_tag="ACHA44_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906030.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2521..>2679)
                     /locus_tag="ACHA44_002819"
     CDS             complement(2521..>2679)
                     /locus_tag="ACHA44_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8906031.1"
                     /translation="NPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKI
                     SLRNWLEE"
BASE COUNT          882 a          498 c          430 g          869 t
ORIGIN      
        1 ttccgttttt tcatccattc ctttcttccc cccttacaaa taaataataa cctttaacaa
       61 ccaactcgta aataggttgg ttgttattat acaaaaggat attagtactc agataaaaag
      121 ttgttagtac tcagataaaa agttgttagt actcagataa aaagttgtta gtactcagat
      181 aaaaagttgt tagtactcag ataaaaagtt gttagtaccc ttctgtatcc gttgggagag
      241 taaggctcaa ctgcccccta aaaagggttc taaaaaatct ttaaaaaata aataaaataa
      301 acgcgcgcgc gcgagcaaaa tttcccgcca aaaaatcacc cactttttaa aataaacagg
      361 ttcgcgcatg atattaaact gccaatctag ctccctatgg tcgctgtaag aaggagtgat
      421 cgctacgctc tcacatttta cttgcagcag tcgcctttag gctccttcgc caaaaataat
      481 tcattaggga ctcctttgtg gcgtcctgag agctcgtcta agccgttcta agacaagatt
      541 cgataaaaac tatgtccttc gttctaaagc gccttacagg cggtcagggc aagccctaac
      601 aaccccaagg caataaaaaa cttaggctta tttttgcttt tttattgtat aattgattgt
      661 cgcctatccg acagtcttgc gactggaagg aggtgtgttt cgtgttcact aactttattt
      721 gctcccttct tgttgggctt ttaagcgctt tgttcagtta ctggttgaat aaccgaaaca
      781 agaagtaaaa cttaattaag gcgactcagc ccacaaaaaa agccccagag aatccgacct
      841 ctctggggct ttcgtgtgtt tcgtgttcac ttatttgcaa tcaaattata acagaaataa
      901 atatttattt caaaaatatc ctacaaaagt ctaaaatccg taattatttc ttatatgcaa
      961 catttacaaa taaagtcgct ctgagagctc gtatttttcg atttaagacg ttttatttag
     1021 tttttggtgt attttcattg gtccaagcta aaaaactctt agaagcgatt ttagagccat
     1081 ttaaacccac ttaattatat tctccatgaa attcgctcca tgcgtcaaaa gctgcgtctg
     1141 aaattccgcc gtcttcgttc actagttgca ttaatttttc caacgatcgt tttctttcgg
     1201 gtgtcatatt tttatattcc aacaattttt cgtctgttaa atcgaacttg attgcttgaa
     1261 ttgcttgctc taatttttct aagttttgaa aatattcgta aggaaatttt tccatttttt
     1321 ctttctccag tctcacaatt tttctagaaa taaaatcact tctttgccgt ctatactctc
     1381 aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc gtgcattttt aaatttggtt
     1441 atcgattaca cgtgcagcac tcccccccga attttaaaga gcataaggaa acgtgtaccc
     1501 actttctaaa aaaagtgggt agataggctg tgcctatcac gtttctcaga aaaaaatttg
     1561 atatcacttt ttcgccatcg tgggtagctt atgtatagca ttttggcaaa aaacagctat
     1621 acaaatatag tatttgtatg gtatacataa attatacata attgcaaaag gagatttttt
     1681 atggacattt tagttcggaa tattgatcca aagtttgttg caacaataga caaacgttgc
     1741 caagaactca cgaataaaac aggagaaaaa tggagtcgaa ataaatatct aaaagtttta
     1801 atcgaaagtg attttgaccg tcaacttacg acttacaaaa aagaccaatt cgatttattg
     1861 ctcgataaat ttattgctat ccaaacagaa aatactcaaa tattaagcga gtatgtctac
     1921 acaaataatc aaatcattga aatgcttatc ggaggaatga tataaccatg agaaaaactt
     1981 ctgtccaaaa acatgttact ttgtacccta gtcatgttga cgttttagat tccatcatcg
     2041 aaaaaaatcc agatatcaat aactactcgg aagccattcg ctttttatgt atgagttatg
     2101 aacccgatac caacacgacc actaccagca atcaggcaaa actaaatgct atgggaaaag
     2161 aaatttccat catgactgaa atcatcgcag aatttgctga tttatatcta aaagatacag
     2221 gcgttcttat tggcacaaat tctaaagtat atcaagaagt gaaagaaaaa atcgaagcta
     2281 aactaaatcc atcaaatcga aatcgaccaa agaatcatca aaaagaaacc agtcaaacag
     2341 tcacttctag aaatttcgac ccaacaaatt ttctaaaacg ataaaaaaca gataggagtt
     2401 attcctatct gttttttatt tagaagcccc atacaatatg ctagaatgta ctagcaatct
     2461 tagtcggttg taagggactt gtctacttgc ttaatgtaag gggggcgagt tcttttttat
     2521 ttattcttct aaccagttac gcagagaaat ctttggcaaa tctgacgtta ggcttgaaaa
     2581 agtaaattct aatttggcga ttttattgcc cttttttgct tttatttttg tgactttaag
     2641 atcattgaag tattttttta gttctcgtaa tgcaggatt
//
LOCUS       DAYYGS010000173         2589 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000173 DAYYGS010000000
VERSION     DAYYGS010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.181"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..2268
                     /locus_tag="ACHA44_002820"
     CDS             <1..2268
                     /locus_tag="ACHA44_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003731151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HFM8906032.1"
                     /translation="EELIDKLDTKDIVNWLNQDEELTDLLSSVKKESSASQQSNWSGL
                     YFNSNEYVKYILNKTAWIKIDGEKYSPRQIVLYEKLQAHVPGLYGVSEQSLMELLGQR
                     IVEQLDFRKNMAMFSDDEIRAILVELPKFDKGEISRRLYNELIRNKKGMNPTYSTEGL
                     SVLAKDGFFYPNKELRYADKRLPKSIEKQMRFIYVPEKASTTTIYEWLGVERFKTNLK
                     LETFELLEDYSEDFKKEIDDIKTAVLSTIDDNSKNVSSLKRIQIIPCSKISAKDVEQD
                     GQSIVLEDYYFVESKGKFYLKLPANSIEINQLRLIDTFSSTIVDIFKQVLNLSLDLNL
                     IELLISRDTEHKQRKITEEFGVDKWNESFELLFSENALNKKVNDFLLSNGLNEEEYPT
                     IFEIDYSYALKEEEYQLLAIALESISKDIEDLNGLSELIHLDIREFWKLKLRSYLDKK
                     AEHYRQILYSNANKSGDEILKETFLEKIDRYKDYQFEISEIPNSVQVDIEKESKKRFP
                     ELSVSITDEIDIDGLYDNNVEELVTVAAITIDDFDYYIRGHQKVSSLLYFGIPSRLRE
                     DILIFVTKENDVDLNGEKSGISIEGTSTVQTILEPSNSVSRKGKNTRNGERSKRDYDN
                     RNSANDDAGETAEQIAYAELTQNQKLNVIWNTKYSSKSSDRNKQPPNGIVCDMWVHDP
                     ENGNMYFEVKSSITEFEMSINEYNSMLDNPDSYEVVLVNRDTKEISRHKFDELDGLKQ
                     ASSYLFKFKQKRSDG"
     gene            2403..2531
                     /locus_tag="ACHA44_002821"
     CDS             2403..2531
                     /locus_tag="ACHA44_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFM8906033.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
BASE COUNT          991 a          339 c          497 g          762 t
ORIGIN      
        1 gaagaattaa tagacaaatt agatacgaaa gacattgtca attggctcaa tcaagacgaa
       61 gaattgacag atttattatc ttcggtaaaa aaagaatcaa gtgcaagtca gcaatcgaac
      121 tggagtggat tgtactttaa ttcaaatgag tatgtgaaat acattttgaa taaaactgca
      181 tggattaaga ttgatggaga aaaatatagc ccgagacaaa ttgtgctata tgaaaagctc
      241 caggcacacg ttccgggatt gtatggagtt tctgaacaaa gcttaatgga actcttgggg
      301 caacgcattg ttgaacagtt ggatttcaga aaaaatatgg cgatgttttc ggatgatgaa
      361 ataagagcta tattagttga gttaccaaaa ttcgataaag gcgaaataag tcgcagatta
      421 tataatgaac ttataagaaa taaaaaagga atgaatccta cttattctac cgaaggatta
      481 tcagtcttag ccaaagatgg cttcttttat ccaaacaaag aattaaggta tgctgataag
      541 cgactgccta aatcaataga aaaacaaatg cgttttattt acgtgcctga aaaagctagt
      601 acaactacga tatatgaatg gcttggggtt gaacgcttca aaacaaattt aaaattggaa
      661 acatttgagt tattggaaga ttattctgag gatttcaaaa aagagattga tgatatcaaa
      721 actgcggtat tatctacgat agatgacaat agtaagaatg ttagttcatt aaaacgtatt
      781 cagattattc catgcagcaa aatatctgct aaagatgttg agcaagatgg tcagtcaatc
      841 gttttggaag attattattt tgtggaaagt aaaggaaaat tctacttaaa attacctgct
      901 aattctattg aaattaatca attacgtttg attgatacgt tttcttcaac aatcgtagac
      961 atattcaagc aggtgttaaa tctaagctta gatttgaatt tgattgaatt gttaatttcc
     1021 cgagatacgg aacataaaca aagaaaaata actgaagaat tcggtgtaga taagtggaat
     1081 gaatcgtttg agctattgtt tagcgaaaat gcgttaaata aaaaagttaa tgactttttg
     1141 ttatcaaatg gactaaatga agaggaatat cctaccattt ttgaaattga ctattcatat
     1201 gcattaaaag aagaagagta tcaattgttg gcgatagctc tagaaagcat ttcaaaagat
     1261 attgaggatt tgaatggatt gtcagaattg attcatttgg atattcgtga attttggaaa
     1321 ctgaaattaa gatcttatct agataagaaa gcggaacatt accgccagat tttatatagc
     1381 aatgcaaata aaagcggtga tgaaatactc aaagaaacat tcctagaaaa aattgataga
     1441 tacaaagatt atcaatttga gatttctgaa attccaaatt cagttcaagt cgacattgag
     1501 aaagaaagta agaagagatt tccagagcta agtgtgtcaa ttactgatga aattgacatt
     1561 gatggcctgt atgataataa tgtcgaagag ttagtcacgg ttgcagctat tacaatagat
     1621 gattttgatt attatattag aggtcatcaa aaagtttctt ccttactgta ttttggtatt
     1681 ccgtcccgtt taagagaaga tatactgata ttcgttacta aagaaaatga tgttgatttg
     1741 aacggagaaa aatctggtat ctctattgaa ggaacatcaa ctgttcaaac cattcttgaa
     1801 ccatcgaata gtgttagtag gaaaggtaag aacacacgaa atggagaaag aagtaaacga
     1861 gattatgata atagaaactc cgcaaatgat gatgctggtg aaacagcaga acaaattgct
     1921 tacgctgaat tgactcagaa tcaaaaactt aatgtaattt ggaatacgaa atattctagt
     1981 aaatcttcag atcgtaataa gcagccacca aacggaattg tttgtgatat gtgggttcat
     2041 gacccagaga atgggaatat gtactttgag gtaaaatcat caattacaga atttgaaatg
     2101 tcgattaatg aatataattc tatgttagat aaccctgact cttacgaggt cgttttagtc
     2161 aatcgtgata ccaaagagat aagtcgtcat aaatttgatg aacttgatgg attgaaacag
     2221 gcaagtagct atcttttcaa atttaagcaa aagagaagtg acggttgagt gatttgtttc
     2281 cacaaaccta ttcaatctat caactcaaaa atcataaaca accattatta tagtaggaag
     2341 atacagagtg tactctcaac gcatatcgaa ttagtcgctt cattcgttaa aggagaataa
     2401 aaatggacca aaaagaaaaa atgaaagaat tgatcaaaaa gaaacaaggt ggaggccgat
     2461 ccaaacaaat ggaaacacct aagaatgatc gtaaaaacat gagaaaaggc cctaaaatct
     2521 ttaataaata gacgtgctaa agaaagacta agaagtgtta acttttgtta ttgtttctta
     2581 gtcttttta
//
LOCUS       DAYYGS010000174         2546 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000174 DAYYGS010000000
VERSION     DAYYGS010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.182"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(296..903)
                     /locus_tag="ACHA44_002822"
                     /pseudo
     CDS             complement(296..903)
                     /locus_tag="ACHA44_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313657.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
     gene            1426..2100
                     /locus_tag="ACHA44_002823"
                     /pseudo
     CDS             1426..2100
                     /locus_tag="ACHA44_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
     gene            2093..2254
                     /locus_tag="ACHA44_002824"
     CDS             2093..2254
                     /locus_tag="ACHA44_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906034.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          900 a          404 c          437 g          805 t
ORIGIN      
        1 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
       61 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      121 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac
      181 tgttgccatt ccatctatga atataaatag agtgtatctt ctttgataaa tgtcagaaaa
      241 agtgtgacaa ataaccatca gtaaaattac ttgtcatact attgataagg agtatttaat
      301 tgaagagaat gattaggaga aaggatctcc gtacttttta tagtaatcat agaatccaag
      361 tttgtataga cgaatctttt tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag
      421 aatcaagtct gtgaattcta taggagctgt tccattcgca gtaatcaaat tacgatcttt
      481 cacagcttgt tgttctataa aatcttcttt attctgataa ttttccgatt ctaaccaagg
      541 aaattgcgca tttccggtgt gtttgtaacc ttgcaataat ccgctctgag ctaaataatc
      601 aactgcccca caaatagcag ctactggtac gtctgttttt aacaatttat cgattatttg
      661 atataaatga tgatttttta tgtcccatga attgccaccg atcataataa aaaggtcaac
      721 tttttcaggt atttgttcga ttaagtaatc gatttttgtt tttaatcctc caatggaagt
      781 gacctctgat tggatagaag cagttttgtt ttgccatccc tctttttggt ttagtgtgct
      841 agtaatataa gcaccttccc aatcggcata ttcctctaat agaaacgtaa tcgcagtttt
      901 cattttcaag ctctccttta ctatctatga caaggacaac agttttaaca tacagacatt
      961 cattaaattt ggatagcgac agcgaaaaca acaattctta agttttagac agagtataca
     1021 gacagtcaac ttaattaaat acgaactaaa taaaaatgac aggtaatcat ttttgtttac
     1081 cagcattata acattttgca tacatggatg acaaaagaac caattcccac ttgcttgagt
     1141 acaatcttaa aagaaactgg aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat
     1201 cgggcactca gacatgaaaa caaccctaga aatatataac tagtccaatg aaaaaaaagc
     1261 gaatgccgta gaaaacaagg attctgcgta cattcatatc tcattttgcg agcatatctg
     1321 ctattactgt gactaaatgt taatatggtt aaaatgaccg ttatattttt tatataaata
     1381 tcgtataatt agtatgaata tagaagaagg aaagaaggtg caatcttgga acctaaagac
     1441 aagtatttag attacgctag ggtcaataaa gataaattta ttgaaacaat catcaaggga
     1501 aaaatccctg atgatgaaaa agatgcggta ttcatggctg gaagcccagg agcaggtaaa
     1561 acagaggttg cactaggttt agcttaaaat tatgacaacc atgttgtgat tgatgcggac
     1621 tattttagag cacaatttcc agaatacaat ggaaagaact caagtgtttt tcagaaagct
     1681 tcttcttggt tagttgaaca atcattaaaa tatgtactgg aacatggcta ttctttcata
     1741 ctagaggata cttttgctat ccttagtgca gagaaaaata ttattcgagc ccttaaaaat
     1801 aactttcgcg ttacgatttt ctatgtttac caagatccca aagttgcctg ggatttcact
     1861 cgaaaacgtg aactagctga aggacgtcat gtgccaaaag aaatgtttat caatgcgttt
     1921 tttaagtcca gagagaatat tgaaaaagtc aaaaatagac atcctgaagt agtccttcac
     1981 ataatgatta aggattatca aaacgacatt tctgaagtgc attatgcagc agataatatt
     2041 caattagtac taccaataca atatacttca aaagagttgg aggaggaatt acatgactga
     2101 gaaagaacaa gtacaacaaa tcgtgaagaa gtataataaa agtatcgctg atttatcaga
     2161 aaacgcctca gcgaaagaat ttaaaacagt aatgaattac gtagcagatg aagcaaatcg
     2221 taagcagcga aaactagttg ggttggataa ataaattttt taaaccatat ccttgtaaag
     2281 ttgagttttt tattttaatg aattaagact attaaataga taattattgt aacttaaaaa
     2341 acacagctaa aaattagttg tgttttttat taggtttact tttctggtat aaaaggaact
     2401 agctactttt tcaaaatttc taaatcattc aatatgtagc gcttactaaa ttattcgcca
     2461 taaaatttta taattggatc actcattgtt ttacctaagt catatttaag taataaccgg
     2521 tgtttttaca taagaagact tcgggt
//
LOCUS       DAYYGS010000175         2407 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000175 DAYYGS010000000
VERSION     DAYYGS010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.183"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            105..2258
                     /locus_tag="ACHA44_002825"
     CDS             105..2258
                     /locus_tag="ACHA44_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFM8906035.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          822 a          424 c          534 g          627 t
ORIGIN      
        1 ttgaaattag gtaaagaatt tcttgctccg cccatttttt cttttttatt aagaagtttt
       61 tgggtacaat gaagcatgaa tgtaaatagg cggaaggata ggaaatgatg aaagatttta
      121 tgaaaaaaat attaaatact tcttggttag aaaaaataag aaaaggtgca caagaaccta
      181 agaacaaaac gtttcgccgg tcccatgttc cttttcgctt aaattttctt tttttcatta
      241 tatttacgtt atttgttgcg ttgattgcta gattgggtta tttgcagatc gtgaacggcg
      301 aagagatgga ggcgcgagtg aagtctactt ctacgattac ggtccaagaa agtactcctc
      361 gcggaatgat ctacgactcc acaggaaaag ctttagttac gaataaagcg aatcaggcca
      421 tcacatatac gagaggaaca caagttacgg ctaaagattt gcttgaaatc gcaacgaagc
      481 tgaactcgct gatcgatgtt ccggtagatg aaaatctgac agatagagat aaaaaagatt
      541 tttggctggc tgatcccaaa catttgaaag aagccacaaa aagactttcg gcaaaagaaa
      601 agcaattagg gaacagcgaa caatatagtg cgatggtcaa taaagtcaca gctgatgaga
      661 ttaatttaaa cgaggagcaa ttaaaaattg ctaccatctt taaacggatg aatagtgcat
      721 atgaattaaa cactgtctat atcaaaaact ctggtgttac tgatcaagag ttagctgttg
      781 tggcagaacg cgcttctgaa cttccaggag tttcgactgg cacagactgg acacgagaat
      841 ataatgcagc aagttcactg aaaagtattc ttggcagtgt gacgactgaa aagcaaggac
      901 ttccagcaga tgaagcagag aaatacctag caaaaggtta ttctcggaat gaccgtgtag
      961 gacaaagtta tttggaaaaa caatatgaag atgtcctgca aggaaccaaa acccaatatg
     1021 aagtttccct tgataatgaa ggaaatgttt cgaatcaaaa agaaatcttc tctggggaaa
     1081 aaggttctaa tttaatgtta tctatgaatg cagaattcca atcaaaagta gaagaaattt
     1141 tgaaaaggaa ttatcaaaca ttgatcaaca acggcaaagc acagtattcg cctggtgctt
     1201 atgcagtggc tatgaatcct cagacaggag aagtgctagc aatgactggt ttcagtcatg
     1261 aacaaggttc caaagaaatc accgagaatg ctttaggcac gattactagc gcctttgatc
     1321 cagggtctgt agtcaaggct ggtactttga cagcaggctg ggcaagtaac gcgatcagcg
     1381 gaaatcaagt tttgattgat gagccgattc gtctgcaagg tgcttcagaa aaaagctctg
     1441 tctttaatcg aagcggacaa gttgctcttg atgcagtcaa agcatcggaa ttgtcttcta
     1501 atacgtatat gatcaaagtg gcgttaaaga tgttaggact agactataca cctggaatgg
     1561 gactacctag tttagatgaa gaagctaaag cttatcagca attgagagac tctttcaaag
     1621 agtttggtct tggtacaacg actgggatcg atttgccgaa tgaatctcct gggatctcac
     1681 gttctgttga ttatatgaag aagttcaatg cggataatgg caaagaatgg tatgctccag
     1741 gtaactttac cgatttggca tttggacagt ttgacacata tacaccaatc caattagctc
     1801 aatatgcttc aacagtcgct aatggcggga aacgagtaca gccgcaccta gtcaaagcga
     1861 tctatggcaa tgacgaaaat ggaaacttgg gtgaagtgaa gaaagaaatt ggcacaacag
     1921 tagaaaatac ggtcaatatt tctgcagaaa atatggcgat cctccgtgaa ggattccacc
     1981 aagttgtcca tggtaccgat ccttacacaa cagccaaacc attagccagt gcgaaaatgg
     2041 acctttccgc aaaaacaggg actgcagaaa cggttgcgga aggtcatcca gatattgcga
     2101 cagtcaatag taatatcgtg gcatacggac caacagacaa tccggaaatt gccatcagtg
     2161 ttgtcttgcc gaacttatta gacgaacaag accacataaa cttgacgatt gctaaagaaa
     2221 taatggacgc gtattatgat atgttcatgg caaaataaaa aagaggtcgt gaaaaagccg
     2281 cttagtttcg agtatgaaga aaaattttgt gaaaatagct tctcacattt ttgcttaaat
     2341 ttgacttcat accgagaaac tggcttttga acaccgttta ctcggcaaaa aaggttgtga
     2401 caaaaat
//
LOCUS       DAYYGS010000176         2395 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000176 DAYYGS010000000
VERSION     DAYYGS010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.184"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            316..570
                     /locus_tag="ACHA44_002826"
     CDS             316..570
                     /locus_tag="ACHA44_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8906036.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="ACHA44_002827"
     CDS             571..888
                     /locus_tag="ACHA44_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8906037.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1447..2382
                     /locus_tag="ACHA44_002828"
     CDS             1447..2382
                     /locus_tag="ACHA44_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906038.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          861 a          339 c          453 g          742 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 ggcaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaaccgt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacatc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 actactttca aaagattatc cgagttaatg cagcagactg ttaaaagtgc tatgcctgaa
     1561 ggatacaagt ttaattttgt tcaatcttca gtatctactg aaagtatcta tttttcaatt
     1621 ctttctgaga gtaggctttt tcattttaga ataagttctc atgtaaagaa aactgatgtt
     1681 gatatatata cgttccgtac aaataattat aaaaatttaa atgatttgag tagaagtatc
     1741 agtgaatatc tcaaggggaa tagctatctc aagattcgat tttcacatat attaatttta
     1801 ctaccatatt tgaatgagaa tttcacccta attcaagtag ataagtcttt cattgcctca
     1861 ggtaatcaag gaaatatgaa tattacaagt aacaaaacgt cacgtgtgct attggcgttg
     1921 atgggttacg gaattcttta tagagatgac caaaatggcc agattctaat aaatcgggct
     1981 gggcgttcat tattaatgaa atatagaaaa tttataccgg aagattttca aaacttacag
     2041 ttagaaaaca tgcgtttaga agatttattt agattagcag aagatgataa gtatagattt
     2101 gaagttttga aaattcaaaa gaatgaacta ttgaataagt tatatcctga gaatgcgata
     2161 aaagcacatt ttggtgatgc taatcatatg tatagttggt tcattcctaa aagatttaga
     2221 gggaaaatca atgctggaga tcaagtgaaa gtactcaatc gaaatagtac agatgtcgta
     2281 atgataacag atatctatgt agaagatgaa gatataatca aatatatgaa gcctgtgata
     2341 aaacttgtta ataaaaataa aggaacccgt ggtgctagtt aattttttcc tagag
//
LOCUS       DAYYGS010000177         2354 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000177 DAYYGS010000000
VERSION     DAYYGS010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.185"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..233)
                     /locus_tag="ACHA44_002829"
     CDS             complement(<1..233)
                     /locus_tag="ACHA44_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8906039.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKS"
     gene            complement(564..797)
                     /locus_tag="ACHA44_002830"
     CDS             complement(564..797)
                     /locus_tag="ACHA44_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HFM8906040.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(812..1762)
                     /locus_tag="ACHA44_002831"
     CDS             complement(812..1762)
                     /locus_tag="ACHA44_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein"
                     /protein_id="HFM8906041.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEVVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(1759..>2354)
                     /locus_tag="ACHA44_002832"
     CDS             complement(1759..>2354)
                     /locus_tag="ACHA44_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFM8906042.1"
                     /translation="IKSNSVIKGDTAIGITFSSFLALGVILIGVANSSTDLFHILFGN
                     ILAVQDIDKWITIGVSVLVLVIVVLFFKELLITSFDPLMAKAIGMNVSFYHYLLMVLL
                     TLVAVTAMQSVGTILIVAMLITPAATAYLYANSLKTMIFISATVGASSSLLGLFIGYS
                     FNIAAGSSIVLTAAFLFVISFLISPKQKFLRKKERSL"
BASE COUNT          753 a          439 c          291 g          871 t
ORIGIN      
        1 caacttttct ctatatctat attttctaag tttaagaggt atttggtaaa ctcttcatta
       61 tacatattaa taagcgcatt aatatgcggc tgaataattt cttcaaataa cgcttcctta
      121 gaagtaaaat gtttataaaa agcacccgta gttacgcctg cggtcaggca cagttgcctt
      181 atcttagttt gctgaaatcc ttctttttca aaatggctta gtccactttc tattaacttt
      241 tggtgagtta taatataatc tctggtttct atgggtactt cctcctaata agatgaatta
      301 actttattat cttacacagg acttgaagtt tcaatcgtta acgtttatta ctgaactcat
      361 tccttagagc taaactaatg agaaagagca gaataactga tacaaaaagc aatcctacaa
      421 gaattaatag actcatctaa atcattcacc ttttctaaaa taatccgaga aagatttaaa
      481 aaaattatat ccagctatta ggataagtcc tatcataatc actccaatag ccattttctt
      541 atcctcccta aaaataaagt cttttactta aaaccttttt tgtaatcgtc aataaatttt
      601 ttccctttat cagttaattg aaatcctata tctaaatagt cctcatatct aagtttagcc
      661 agcatctcac ttacagatgc tggactaaca cttagaactt ttgctaaatc attatttttt
      721 aatttctttt ttctttttga taattcctga attgctagta aataaattag tatagtagtc
      781 gttttaattt gtaccatacc taccctccaa attatttcga aaggccttca gcaattttat
      841 ccaggttcca tttcatcatc gcatagtaac tatcaccatc ttgtcctttt tctgcaattg
      901 aatcagtaaa aatcgttgaa tagataggaa tattggtatc ttttgatact gttttcattg
      961 gtctatcatc cacactgctt tctacgaata aggagggaac ttttgttgtg cgtaattttt
     1021 caactaagtg ttttatttga tctggtgttc cttcttcttc agtgttgatt tcccaaatgt
     1081 aagcagaagg cacattatac gctttcgaga aatatttaaa gcatccttca cttgtaacaa
     1141 tcattttttt atcattcgga attgaagcaa atttagattt agcttctttg tctagagaat
     1201 ccaatttttc aatatactta tctagatttt ctttatagaa ttttttatta tcaggatctt
     1261 tttccgctaa ctgtttttca atatttttag cgtaaataat accgttttct aaatttaacc
     1321 aagcatgagg atcttccttc cctttctcac tctgaccctc taagtaaata acatctatgc
     1381 catcacttgc tgcaaaatag tctttgtttt cctctttgtt cgcattttta actaattttg
     1441 taaaccaagc atttcctcca gtttccaagt taacaccgtt ataaaaaatc aaatctgctt
     1501 ttgaagtttt ttgaacaact tcaggcaaag gttcatattc gtggggatct ttcccaatag
     1561 gtacgatact gtgtaaatca attttatctt ttgctatatt ttcggtaata tctgctaaaa
     1621 tcgaattagt aactactact tttaattttt cgttcgaagc cactttatct ttgtctttac
     1681 tatttattga acatgccccg aatataaaaa gagcaatcaa aatccccaga cttcccagta
     1741 ctgctttcca ttttctcatc ataacgatct ctccttcttt cttaaaaatt tttgttttgg
     1801 tgagattaga aaactaatta caaataagaa tgccgctgtt aaaacaatac ttgatcctgc
     1861 tgctatgtta aaactataac caataaacaa tccaagcaat gatgaacttg ctccgactgt
     1921 tgcagaaata aatatcatag ttttcaagct atttgcatat aaatatgctg tagctgctgg
     1981 tgtaattaac attgcaacaa ttaatatagt tcccacactt tgcatagctg ttactgctac
     2041 taaagttaat agaaccatca atagataatg ataaaaactg acattcatcc ctattgcttt
     2101 tgccattaat ggatcaaaag aagtgattaa taattcttta aaaaatagaa ctactataac
     2161 caacactaaa accgaaacgc caattgtaat ccacttatca atatcttgta cagctaaaat
     2221 atttccaaat aaaatatgaa ataaatccgt tgagctattt gcaactccta taaggataac
     2281 tcctagtgct aaaaaagaac tgaaagtaat accaattgct gtatctcctt ttattacact
     2341 attgcttttt ataa
//
LOCUS       DAYYGS010000178         2281 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000178 DAYYGS010000000
VERSION     DAYYGS010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.186"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            88..1950
                     /locus_tag="ACHA44_002833"
     CDS             88..1950
                     /locus_tag="ACHA44_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFM8906043.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            1950..>2281
                     /locus_tag="ACHA44_002834"
     CDS             1950..>2281
                     /locus_tag="ACHA44_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFM8906044.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          818 a          340 c          450 g          673 t
ORIGIN      
        1 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
       61 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      121 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      181 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      241 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      301 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      361 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      421 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      481 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      541 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      601 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      661 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      721 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      781 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      841 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
      901 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
      961 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1021 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1081 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1141 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1201 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1261 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1321 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1381 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1441 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1501 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1561 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1621 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1681 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1741 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1801 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1861 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     1921 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     1981 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2041 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2101 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2161 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2221 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataatt
     2281 a
//
LOCUS       DAYYGS010000179         2257 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000179 DAYYGS010000000
VERSION     DAYYGS010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.187"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(76..1095)
                     /locus_tag="ACHA44_002835"
     CDS             complement(76..1095)
                     /locus_tag="ACHA44_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFM8906045.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(1114..2115)
                     /locus_tag="ACHA44_002836"
     CDS             complement(1114..2115)
                     /locus_tag="ACHA44_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFM8906046.1"
                     /translation="MTSTVFIILLGFVCRKKGIFSESVGKILSKVVLTVALPALAFRA
                     FMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYKGDKQTTLRVLTIFGSTTFF
                     GTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGLKMEAKNIKAMFVNPIIIAT
                     FAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPWLFQPMDYLAGLSSPLAWLA
                     IGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILAVLTATNILPVNFEALATIV
                     IMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVLTPVWIVVLQMIHGIGLFS"
BASE COUNT          701 a          480 c          379 g          697 t
ORIGIN      
        1 gtccaagtat acagaagtaa caactgttta cttggactat ttttaaacat tttattgacg
       61 aatatccatg ttttcttatc ttaattgacg ggctttagga agagatacct catttttcgt
      121 ttttccagtt tctattattt cacgaaagtt ctcgaaactt gtcgaaatca tatttgataa
      181 tgcctcatct gtatttgaac ctacatgcgg ggttacaaga actctagggt acatttctac
      241 taattgctgg atagcagggt ctggaatcgt ctcccatggc ttgaaggatc ggaaaaaaac
      301 ctctgtttca tttgcaaaaa catcggttgc aaaaccagct aaatgattgc ttttcaaagc
      361 tcttaaaata gcttcattat cttgaagctc tccacgggct gtattaatca agatgctacc
      421 ttttttcatt tgagctaaaa atccttcatt gatcatttga tcgttttcac ctgggatatg
      481 aggtacatga atactgacaa tatcgctttt ggctaataac tcttcaagcg agtggaacgt
      541 caatactttt ttagcagctt ctgtctgatg aatatcgtag ccgatcactt tggctcctag
      601 tcccttaaac aaaacagctt ccgtcaaccc tatcctgcct gttcctatga tccctactgt
      661 gcaatttctg atttcacgac taaacatttg ctcatccact cgaaaatctt tgaaagatgt
      721 tcggatcgtc gtatacgcag tatttctcaa aagcatcatg gcaaaagtca aagataactc
      781 tgctatcgca tttggcgaat acgatggtac acgggctact gtcataccaa aatcagctgc
      841 cgcttgtaga tcgatatgat tgactcccac agtccgtgta aaaacataac gaatcccata
      901 ttcctgcatt tttgcaagat tggtctcatc tgctatacaa tttcccctca acaatacagc
      961 atcatggtcc tttgctgaca cgatattgtc atgtgtcaac agttcttcat aaagactgag
     1021 atcataatgg taaatattca atttttcaaa aaaatctatc tcgttcggac gaacaccgta
     1081 acatgcgatt ctcatacttt cttcctccat ttcttatgaa aataaaccta tgccatgaat
     1141 catttgtaat acaacgatcc atacaggtgt cagtaccaca gcaaaaatag ttgacagtaa
     1201 ggatgcattg gaggctaaaa gtgcctccct atcaaaactg atagcatagg ctgctgccac
     1261 tgttgcagct ggtgtagcca tcatgataac gattgttgcc agtgcttcaa aattgactgg
     1321 tagaatattc gttgccgtca aaactgctag aatcacgata ttcaatgcag gtacaaatac
     1381 cactttgact agactgtaat accatgaagt tttttcagat gcagcttttt tgaagcttac
     1441 ttcacctaat gttgcgccga ttgctagcca cgccaatggt gaacttaaac cagctaaata
     1501 atccatcggc tgaaatagcc acggtgcagt ctggtcaatt cgcaaaaacg ctacttggct
     1561 tatctctcct tctgcacctg taactgacac ttgtggcaga tacccttgga acagccaaat
     1621 gaaaaggccg gcaaaagtag cgataatgat tggattgacg aacatcgctt tgatattttt
     1681 agcttccatc ttcaagccac tcatttttat gtatccataa gaatacaaaa agattcggta
     1741 gccaatattg aaaattgagg aatacatcac gccgacagga ccataaatcg cactgacgat
     1801 aggcgttccg aaaaaggtcg tagaaccaaa gatcgtcagg acacgcaacg ttgtttgctt
     1861 atccccttta tagcttaaga aaaaaggttt tgaaactaga ataagaataa tataaataac
     1921 taatccccaa atcaagacat tgattccttg catcagtaat tctgtattga tatcttgcat
     1981 gaaagctcga aatgccagtg ctggcaaagc cactgttaag acgactttcg atagaatttt
     2041 tccaacagat tcagaaaaaa tccccttttt tctacaaaca aaccctaata atataataaa
     2101 aactgttgaa gtgattgcac tgacaatcga catatctgtc aaagtcgatc gaaaaatgtc
     2161 aaataagttc atctttctcc tcctaattat gttcatttat tcacaataaa aaaagctttt
     2221 attttgctaa tacgtattaa catttttaat tttatct
//
LOCUS       DAYYGS010000180         2217 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000180 DAYYGS010000000
VERSION     DAYYGS010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.188"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            157..990
                     /gene="msrA"
                     /locus_tag="ACHA44_002837"
     CDS             157..990
                     /gene="msrA"
                     /locus_tag="ACHA44_002837"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFM8906047.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            1070..2059
                     /locus_tag="ACHA44_002838"
     CDS             1070..2059
                     /locus_tag="ACHA44_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFM8906048.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
BASE COUNT          804 a          365 c          442 g          606 t
ORIGIN      
        1 aaataaattg attaaaaagg agaatctgtc gataaggatt ctctcttttt tatacgaaaa
       61 atctgtaggg ggatgaagta atcgcttatt tcctcacttt atgataaatt acagctaaag
      121 tcactagctt gtcttcttcg gaaaggagga gaatctatgg atgaggaaca aatattcatt
      181 caaacacttt ataatctgat tttgaatccg aatacacgag attgggaacg aaaagtattg
      241 attcagacaa agaatgacat aagagaaaat atatccgtta aagaacagtt atcaaaacta
      301 gaagcaacac tacgtccatt agctatccga atgaatctga cgcctgatgt aatggacttt
      361 tatttattga ttacggaagg atttgataag gagcaaaaat atgacttttc caagcatgcc
      421 atgcaagatg ctgactatca agaacgtgca gtatttgcag ggggatgttt ctggtgtatg
      481 gtcgagccgt ttgaatcgaa aaaagggatc ctatctgttt tgtcaggata cacgggcgga
      541 catgttgaaa aaccaaatta tgatcaagta agtggtggtt acacaggtca tgtggaagct
      601 gtggagatca tttatgacac aagagaaata agttatagcg agttgctgac aatctattgg
      661 caaattactg atcccacaga tacatttgga caatttcaag atagagggaa gcaatatcgt
      721 ccagtgattt tttatcaagt cgaacgacaa aaagaattag cggaacaatc aaaacagaaa
      781 ctagacagtt caggaacttt ccaccaacca atcgtcacaa aaatcgaacc agccggtacg
      841 ttttggccag cagaaaacta tcatcagcaa ttttacaaaa aacaaccgaa acgttataaa
      901 aaaatccagc aagcacgtaa tcaatttctg atctatcaac gagtaaaaaa caaatggcaa
      961 aaaaatatac gaaaaaatca ttttgattaa ctccttttct ttaaaatgta gtaaactaaa
     1021 aatagaaaaa agaaagcgtt ttaaaaagat ttgatgagga ggagaaaaat tgtatattgc
     1081 agatagtgca cgttatgaaa aaatggaata tcgtaaatcc ggtcattcag gtttgaaatt
     1141 accgatttta tctctcggat tatggcaaaa ttttggtgat tatgatccta tccataatca
     1201 aagagagatt ttaagaggcg cattcgacat gggaattacc catttcgacc ttgcgaataa
     1261 ttatggaggc cctgctggag ctgcagagaa aaactttggg cgtattttta gagaagattt
     1321 tcaagcctac cgtgatgaac tgattatttc tagcaaagca ggttatcata tgtgggaagg
     1381 gccatatggg gaatggggct ctcgtaaatc catcatttca agttgtgacc aaagtctcca
     1441 aagaatgggg ctagattatg ttgacatctt ctatcatcat cgcccagacc ctgatacacc
     1501 tttggaagaa acagcagaag ctttgatgca acttgtaaga caaggaaaag cattatatat
     1561 tggtatatcc aactataatg gagaagatac aaaaaaaatg actgaaattt taaaaagaaa
     1621 ggaagcaccg ttcattattc atcagatgcg ctacaatatg ttttctcgtg cactactaga
     1681 agacgatcta tctccagtat tagaagaaga agggctaggg gcaatcacgt tcagtccgct
     1741 tgctcaaggg ttgctgacaa atcgttatct acatggtatt cctgaagatt ctagagcaca
     1801 tcgtaaggaa ataccattcc tttcagaaga acaagttgga tcaactctgg aaaaaatcaa
     1861 agcactgcaa acaatcgccg tatcacgagg acaatcatta gcgcaaatgg cccttgcatg
     1921 gaatttgcga caaaaatctg ttacaagtgt actggtcgga gcaagtcggt taagtcagtt
     1981 acaagaaagt gttcgaatga tggataattt agatttctca ccagaagaag aattgagaat
     2041 cgaccaaatt ctagaataga aacgatgtaa tcaaactttt tatccatata ttaaaaaaaa
     2101 caaaagatga catcaagaat cgaatgaaga tcgtctgtta ttgaacatca tattttttgt
     2161 cgttcaaaat aaaaatgatc gaaggctagg agtgaatttt atcctagtct tttttat
//
LOCUS       DAYYGS010000181         2148 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000181 DAYYGS010000000
VERSION     DAYYGS010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.189"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            54..542
                     /locus_tag="ACHA44_002839"
     CDS             54..542
                     /locus_tag="ACHA44_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HFM8906049.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            838..1119
                     /locus_tag="ACHA44_002840"
     CDS             838..1119
                     /locus_tag="ACHA44_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906050.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            1121..1537
                     /locus_tag="ACHA44_002841"
     CDS             1121..1537
                     /locus_tag="ACHA44_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906051.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT          848 a          389 c          324 g          587 t
ORIGIN      
        1 aagaaaactt actagataat ctccaaaact tatttaaaga ataaggggtt tttatgacag
       61 ataaaactat caaagaatta gcagatattc taggtgtttc aaaagataaa gtgaagtatc
      121 aactgaggaa attacctagt aattttacct acaaaaaagg aaatattacc tacctgaaaa
      181 aagaagcagt aacaacaatt acaaaggttt tatcaggtaa tgaatcgggt aacttaccta
      241 gtgaattacc cgattcatta cctaccgatt acctactcaa ggagattgaa actaagaata
      301 atcatattga agaaaaagat aggcaaattg aaaaacttca aatagcttta gaaacgcagc
      361 aaaaattact cgaccagcaa caacaactca ccctacaagc caatcaacaa atcaaaaaac
      421 ttcacgaaca actccagcta acctatgcag aagaatcttc agaaaaagaa cccctttctg
      481 ttactgaaga aactcaaaaa ggacaaaaca aggagaaaaa atggtggcaa ttctggcaat
      541 aacccaacaa tttttattca taaaaattgt tgggttactt tctttttttg cttttacaag
      601 catagcgaca tgatctcacg tcgctatttt ttaaagagca taaggaaacg cggtatcact
      661 ttcttacgaa aggtgctagg taggctgtgc ctaccacgtt tctaagaaaa aaatttgcta
      721 tcactttttc gtcattgtgg atagcttatg tatagtgttt cggcaaaaaa cagctataca
      781 aatactatat ttgtatgata tacataaatt atacacaaat gcgaaaggaa ttcttctatg
      841 gacattttag ttcggaatat tgatccaaaa gttatttcag aaatagatag acgttgccaa
      901 gaactcacaa caaaaactgg caaaaaatgg agccgtaatg cgtacctaaa agttctaatc
      961 gaaaacgatt ttgaccaggc acttaggcat tacaaaaaag atcaattcga tttattactc
     1021 gaaaaattta tcgatataca agcaaaaaat actgaaacat tgaacgacta tgttcataca
     1081 aacaaccaac tcattgaaac acttatcgga ggaatataac atgagaaaaa cttctgttca
     1141 aaaacacgtt accctttatc ccagtcacat tgacgtttta gattccatca tcgaaaaaaa
     1201 tccagatatc aataactact cggaagccat tcgttttcta tgtatgagtt atgaacctaa
     1261 tactaatacg acaactacca gcaaccaagc aaaactaaat gcgatgggaa aagaaatttc
     1321 tattatgtcc gaaatcattg cagaatttgc tgatttatat ctaaaagata ctggtgttct
     1381 tatcggtaca aattctaaag tatatcaaga agtgaaagaa aaaattgaaa atcaactcaa
     1441 tccctcccga cgaaatcaac ccaaaaatcg tcaaaaagaa accaatcaaa cattcacttc
     1501 tagaaatttt gacccaacaa actttctaaa aagatgaaaa agaaaccgaa aattttttct
     1561 atttctcagt ttctttttta gggggcagtc ttccagcaag atttgcaagt taccggtaac
     1621 ttgcacaaaa aaagaaaaat ccgcttgtgc gaatccttct ttttgcattt tggggcgctg
     1681 ccccaatctc caaatatcgg ttatttaata gcgtggtaac tcaaaaaaat agaacgtact
     1741 ctgaaataaa aaaagactgc tctaacgagc agccaataaa aaataatgag cgtttattct
     1801 tgaaaaagca gcgcgcttat gaagcgctgc caaaaattta ccatatttta tgaaaaataa
     1861 caagtttcta aaaaggaatc ggttaagatt cacaaagaat cgtaaacaga caatagcgga
     1921 aaatcaagct tccgaacgta ccttttagct agaccaatga aaaatcacaa agtgagcggc
     1981 tggatccctt ggtacaggcg ttacttggta gtatacacat tatgttaact aacgctataa
     2041 ataacgatta tgttcccaaa acaatatttt atgaactata acaaaaattt gttatcgcat
     2101 attgtatatt ttaattatag gtgtatacca cttaaattta acacaaag
//
LOCUS       DAYYGS010000182         2115 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000182 DAYYGS010000000
VERSION     DAYYGS010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.190"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..353)
                     /locus_tag="ACHA44_002842"
     CDS             complement(<1..353)
                     /locus_tag="ACHA44_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906052.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="ACHA44_002843"
     CDS             complement(613..1161)
                     /locus_tag="ACHA44_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906053.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="ACHA44_002844"
     CDS             complement(1162..2019)
                     /locus_tag="ACHA44_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFM8906054.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
BASE COUNT          584 a          449 c          269 g          813 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac tacct
//
LOCUS       DAYYGS010000183         2080 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000183 DAYYGS010000000
VERSION     DAYYGS010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.191"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..426)
                     /locus_tag="ACHA44_002845"
     CDS             complement(<1..426)
                     /locus_tag="ACHA44_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFM8906055.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(535..1566)
                     /locus_tag="ACHA44_002846"
     CDS             complement(535..1566)
                     /locus_tag="ACHA44_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8906056.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          645 a          336 c          360 g          739 t
ORIGIN      
        1 aggaatctgt tttgattttg ttccatttga aatttctctc actcgctttt ttgtctcatc
       61 atcaaataaa atgaaagggt cttctaaacg atcgataaat acttccattg ttgttgttgg
      121 tagatgataa gaagtcatga caggcactgc accaaggtaa gacacagaaa cagctagaag
      181 gtatgtgtca aaagcggaac tcttataaat aataactttg tctccggatt tcactccaag
      241 atgagccaat tgataagccc gtttcaacac actttcgcag cattttttgt aagttgtttc
      301 aagtcctaat tctgggaaag ctgaaagtgg ctcatcaaag ataatcgggg tctcggaaaa
      361 gttagctgca gcttcttgaa aattcgtaaa taagttcaaa ggggaatacg ttatggtctc
      421 catcattcac actccattat cttttgttaa gtaatataca aattcaagat aatcgtaaca
      481 taatccttta tataataaca tagtttattg tgaaaaaaag atcatacatt tgttttatat
      541 ggaagaaacc cgaacgaatg ccgctaattc gtgataagaa aaatcaacca tcttttccca
      601 gatatcttga aaagaatggg atgagaggta tcgattacca cataaataat ttaccgcgtc
      661 agcatcatct aaatatagtt ctgcataata acgatatgtt tttctgcata gttctggcga
      721 tacgttttta tatactgtaa ttttgctcaa aatttggcga atagtccgag gagaaatttg
      781 ctgatcatgt gaggaacgaa aaacaaaaga attttcgtcc gtattttgtg ctgttaactt
      841 aaaatagttc tccagaaatg gcatcgcttc ttttggtatt ggtactttat tcagtctgtt
      901 ttcttttcta ttcagattaa gaatctgctt ggtcaaagat acatctttac gtttaagttc
      961 tactacttca tggatcttta atccgcaata cagaagaatg gcagtaattg caaggttcct
     1021 cggtctattc ttttcttcaa agctagcttc tctgcttgtt tgaaagcctt caatcgggat
     1081 aaacaaatgc tcaaaatctt tgaaaagttc tgcatcgata tatcgcggag ctgctttggt
     1141 tgtttccggt aaacttgtac ccaaaagaga aatctcttct tcaatcagtc ctttttgata
     1201 caaaaatcgt aaaaaatgat ttaatgaata ttgcttcctt gaaatcccgt aatagctatt
     1261 ttcaaacaat tgctccgttt cttggttcgc tgtagtttgg aacgaacgag tatgtcgata
     1321 gattttagct aagaaattct gataatcttg tttttctaaa tggataatca gctgatcatc
     1381 cacttcttca gaaaatactt ttctcaaata atcagtaaac agatgaaaat cttttgcgta
     1441 ttcatagcga ctgcctaatg aaagggaaga acgattttct tctaaaaact gttgaaagac
     1501 ggaagggagc aagtgtgtac gttgctttaa cttttcttgc agttgttttc tagttttgct
     1561 gtccattaca aattccctcc aatagtgttc attttctata ttttaccgca tttcatcaaa
     1621 tgaagataga cagtttgtct tgatcttagg aagcaaattt ttaactaagt actagctaaa
     1681 agatatgaag taagaagata catttttagt tattttatta gtgcagtcat tcttttgatc
     1741 ttttaattga tttatcctct ttttagctat gtggttactt catgagcaat gaaatggatt
     1801 tggttcgttc taatggcagg aggctctttc ttttatttcc gaaaaattag aaaacatttt
     1861 ttcagaatcg attggctctc catttattca ataatggttt gtctaacgat attgttggga
     1921 taattcttaa agaagtatta gaaatagttg gtacttcatt actttacatg aagatttatt
     1981 ctatggtagg agcaattata ttgagaatta atcctttttt atataagttg tttgcttatt
     2041 gatggattgg ataaattaaa ccatcacaac gagtggaaat
//
LOCUS       DAYYGS010000184         2063 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000184 DAYYGS010000000
VERSION     DAYYGS010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.192"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..244)
                     /locus_tag="ACHA44_002847"
     CDS             complement(<1..244)
                     /locus_tag="ACHA44_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8906057.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELI"
     gene            complement(237..1829)
                     /locus_tag="ACHA44_002848"
     CDS             complement(237..1829)
                     /locus_tag="ACHA44_002848"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFM8906058.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
BASE COUNT          595 a          424 c          324 g          720 t
ORIGIN      
        1 tgattaattc attatatttt ttttcagtaa ttttatatct tgtttcgtaa ttatcataaa
       61 tagcattttg ttgttcatag acaacaaaat tgctattaga tacaaagaat tctttcttta
      121 aggcactccc aaatggtccc ctccttactc catctttttt atcaagtaag cattcaaact
      181 tacgctgttc ccaagcttcc gtaaatcctg ggaaacgaat ttctggttgt atatcattac
      241 tcatgtttga acacctccag cgcaccttgt aaccacgctt cattttcagg tgttgtttgc
      301 agggaagaga tagcttctaa aagttctttt tctagcactt gctgttcttg tcgtatcttc
      361 ttgatgtcat ttccgacatg aaccatatca attggttctt cttcttcaaa cgtatctaca
      421 tagcgtggaa tatttaagtt atagtcattt tctttgattt catcaaagtt tgccaaatga
      481 gcgtatttct cgacatcttt tctttcagca taggtttcga ggattttttg aatgttttct
      541 tccgataatt tattctgatt ttttcctttg acaaattctc gactagcgtc aatgaataat
      601 acgtctcgag tttggcggtt ctttttcaat acaatcaccg tcgttggaat cgatgtcccg
      661 aagaacaagt tcgcaggcat accgatcaca gcataaatac tgccgtcttc tagtaatttt
      721 tgacgaatga ccccttctgc agcgccacgg aataaaactc cgtgtggcaa gacgatggcc
      781 atagtccctg tttccttcaa atgatagaaa ccatgaagaa gaaacgcaaa atctgctttg
      841 gattttggcg ctaattttcc ataacgatta aaccgagaat cgtctaagaa ggtcgtatct
      901 gctgaccaat tagccgaata aggtgggttc attactactg catcaaatgt gtaaggttca
      961 tctgttggcc aatccttatt caacgtatcc ccattgcgga gattcatttc ttcggcatct
     1021 accccatgta aaatcaagtt catttttgct agattgtatg tcgttgtatt taattcttgc
     1081 ccatgatatt taacgttatc tgggtgagtt aagtagttac gcacatttaa catcaatgaa
     1141 cctgaaccca tggttggatc aaaaacacta aagaatcgtc gttctttttg atccaacgtc
     1201 acgatttgtg ccatcatatc agaaaccata tgaggcgtgt aaaattctcc ggctttcttc
     1261 cctgcttctg aagcaaattg actgatcaga aattcataag catccccgat cacgtcccca
     1321 tcgtgaccta aaacttccac gtcattcaat ttcttaatga cttcggtaat cgtcacattt
     1381 cgttgttgct catctgttcc tagttttttt gattggagat ccacatcatc aaataaccca
     1441 ttaaattgat tgtaggtaga tgccaattgc acaaaggctt tgtttagatc attcaattgg
     1501 aacgttgctt gttttgcttg atccgctaag acgttgaaaa gatactctgg cgaaatcgca
     1561 taccccaaaa tatcaacgat tgttgcaatc agatcttctt ttgattcttc atctgaaagt
     1621 aattcgcgat acaacatcgt ttgtttgctt accgtatcat attcctctaa ggattcatct
     1681 gctaaaagaa ccacttgttc caacaaacgg tcggataaat atttatagaa aatcagtccc
     1741 aataaataat ttttatattc tgacgcatcc attttgctac ggagattatc tgccgcacta
     1801 aataattttt gatttaattc tgcacccaaa aagatacact cctatctctt atttaaagtt
     1861 gctatatacc ctcttatttt agcatactcc cttttagaaa aagcaaaaaa aaaaagaaag
     1921 aaacttattc ttgctaataa actacgcgaa aggagtctaa cgttgtttga tataacttag
     1981 actgatcgtt aacttatttt ttatatttct acatagttat ctttagaacc atttcagata
     2041 catatgaaac aattacatat gat
//
LOCUS       DAYYGS010000185         1986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000185 DAYYGS010000000
VERSION     DAYYGS010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.193"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="ACHA44_002849"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="ACHA44_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HFM8906059.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="ACHA44_002850"
     CDS             853..1041
                     /locus_tag="ACHA44_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HFM8906060.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="ACHA44_002851"
     CDS             1054..1611
                     /locus_tag="ACHA44_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HFM8906061.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="ACHA44_002852"
     CDS             1643..1885
                     /locus_tag="ACHA44_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFM8906062.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          724 a          306 c          406 g          550 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt tatatgaagt
     1981 attctt
//
LOCUS       DAYYGS010000186         1953 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000186 DAYYGS010000000
VERSION     DAYYGS010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.194"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..161
                     /locus_tag="ACHA44_002853"
     CDS             <1..161
                     /locus_tag="ACHA44_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFM8906063.1"
                     /translation="SFEENIVKEMPESANDMYEKELKYFFENVVTGKENWNDLYHANE
                     VLKIAEEK"
     gene            162..914
                     /locus_tag="ACHA44_002854"
     CDS             162..914
                     /locus_tag="ACHA44_002854"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFM8906064.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            919..1866
                     /locus_tag="ACHA44_002855"
     CDS             919..1866
                     /locus_tag="ACHA44_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFM8906065.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          681 a          263 c          390 g          619 t
ORIGIN      
        1 taagttttga agaaaacatt gttaaagaaa tgcctgaatc tgctaatgat atgtatgaga
       61 aggagttgaa atatttcttt gaaaacgtag taacaggcaa agaaaactgg aatgatttat
      121 atcatgcaaa cgaagtattg aaaatagctg aggagaaata aatggaaatt ttatttacta
      181 tttgtggtag agctgggtct aaggggttta aaaataaaaa tttgaaatat ttggcaggaa
      241 agcccctagt ccatttcgct ttagcaatga tagatatcta tcaacaaaaa catcctgaac
      301 ataattgcac gacttgtttg aatacggaca gtaatgagtt agtagaattg acgactaagt
      361 taaatccgtc tgtgaaaatt gtagaacgta aacctgaatt agctacagat actgtaggga
      421 aaattgaagt gatacgtgat tcattcttac aactagaaag agaagataat atatttgatt
      481 taattataga tttagatttg acttcacctt tacgtgaagt ttctaatctt gaagaattaa
      541 tagatgaaca tctaaaaaat ttagataaag acgtctactt ttcagttgtt tctgcaagac
      601 gtaatcccta ttttaatatg gtaaaaagaa ttgaaaatga agttactata gttaataaat
      661 caagatacac atctaggcaa cagacacctg aagtttttga aatgaatgcc tcaatgtatt
      721 cttttaaacc tgaatttcta aaaaaaagta attatatttt tgatgggcaa tgtgggataa
      781 taaaaatgag agattatcta attttagata ttgatagtga agaagatttt aaatggttag
      841 aatttttgtt tcctaaattt ttagaagatt cttcggagct tcaagatatc tataattatt
      901 tatatcggag gtaaaaggat gagctgttat cttatcaccg gtggggctgg cttcattggt
      961 tctaccttag ccaatcattt aggagaagaa catgtggtaa tcgtcgtcga tgacttatca
     1021 atgggaaaag tagaaaatct tgatatgacg aaaaatatca cttttattga gggagatgtc
     1081 gctgagaaat ccttgatgga agagatcatg aaagctaata aatttgatta tatctttcat
     1141 ttagcagcag ttgctagtgt ggcagattca gttgcccgac cagtagaaac acatcgtgtg
     1201 aattttgaaa gtgtattaat gattttagaa ttgatccgca agtatcaacc tgatttaaaa
     1261 cggatcgtgt ttagttcttc agcggctgtt tatggggatg aaccgacttt accaaaaaaa
     1321 gaagagtccg tgattcgccc attgacgcct tatgcggttg ataaattcgc tgcagaacaa
     1381 tatgtgctag attattgtca tttatatgat gtacccggta gcgcagttcg ttttttcaat
     1441 gtctatggtc caaatcaaaa tccgaattct ccatacagtg gtgtgatttc tattctcgta
     1501 gatcgctata agaaacaaat cgtaggagaa gcaacttcat ttacattatt tggtgatgga
     1561 agccagtcta gagactttgt ttatatagat gatgtaatcc aagcattact tttagttgcg
     1621 aatgaggaag ctgcactggg gaaacaattc aatgttggaa cgggtaaagc aaccacactt
     1681 ttggaattga ttcatgcaat caatgaaatt ctcggtgtag aattgacact tgaatacaaa
     1741 gaagagcgtt cgggtgatat ccatgactct ctagcagata ttactaagat ccagtcaatt
     1801 ggctatcaac cgaaatatgg tattttaagt ggaatgacta attatttgaa aactgaaatc
     1861 aattaaaaca tactaagata agattagagg gtaaattcag gctctttgtc aataaggact
     1921 gatgagtttt gtaaaatcaa atctgggata gaa
//
LOCUS       DAYYGS010000187         1905 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000187 DAYYGS010000000
VERSION     DAYYGS010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.195"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..963
                     /locus_tag="ACHA44_002856"
     CDS             <1..963
                     /locus_tag="ACHA44_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFM8906066.1"
                     /translation="PINGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRN
                     EAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKA
                     TFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSE
                     MGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNYAHIDQFGEIE
                     NANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRADVNQAYNTSGNVGYHVS
                     FNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="ACHA44_002857"
     tRNA            1152..1225
                     /locus_tag="ACHA44_002857"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
BASE COUNT          636 a          335 c          362 g          572 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttatcaa gtcggtcaac ctggttatgt acagtacggt
      241 gctggttcgt atgctaatgc caacagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaacgt ttgagaaaaa ctacaaggca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac gttggacact ccttataacc aaccgggaat caaatcgcat
      421 ttatgggtaa cacaaaacat ctggggcgat catacagatc cttacggtta tctttctgaa
      481 atgggcgtaa gtaaagaaaa attagcatat gatttagctc atggatttac cgatgaaaat
      541 ccaacaactt ctgaaaacaa gcctgtcatt gatccaacac gagctggtgc agctaatcct
      601 acactgacag atggaacgaa ttacgcccac attgatcagt ttggagaaat cgaaaatgca
      661 aatttgcatg tagctggatg gcacattgct aactataaat acgagtatat cttcattatg
      721 gattacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagagcagat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt acaattggag tggtagttaa ttagtgactt atttttgatt ttatagcact
     1621 gatactataa aatatagata tcatcatatt acacaatctt aatactaact taaaaaatat
     1681 ctcctttcac aagtatggtg ataaaattcg ttccgggcta cctttttagg tagcctactt
     1741 taatctttat acctttctgg atcaacgaaa gtatacttta tatagtcata acgccgatga
     1801 tcgctacgtg cgtccggcac gtcagtcatg aatcggctac actagactag acagaaaaaa
     1861 taaggtgtgt agaataataa aaaacacact ggaggatttt tcatg
//
LOCUS       DAYYGS010000188         1887 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000188 DAYYGS010000000
VERSION     DAYYGS010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.196"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..181
                     /locus_tag="ACHA44_002858"
     CDS             <1..181
                     /locus_tag="ACHA44_002858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906067.1"
                     /translation="GIWLANIELAKPFSVFCRKGPYSHFHITNDTSIGRIKRVGLMSK
                     LKQNLASNPSKKYTY"
     gene            958..>1887
                     /locus_tag="ACHA44_002859"
     CDS             958..>1887
                     /locus_tag="ACHA44_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFM8906068.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     NFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIQKRYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKEPTSLQEKIKEQKRKLELL
                     QQLAELERKQNTPSKKINKQIADNNSDITPEFKE"
BASE COUNT          658 a          313 c          348 g          568 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtccata ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga acaattaaaa gatttaagca attataaagc aactgcaaat
     1141 gttcgattca tagatgattt agaaacaacg tatgacaaat taatggattt acgttttggt
     1201 agaagaagtg ctgacggttt gcaacgtgaa cgatttgttt tatttaatca atttaaaatc
     1261 gatggaaaag ctgatattcc ttttgctgaa atacaagtcc atgagaaagc cttaccctta
     1321 cttaataatc ttgaagaatg ggttcgatat tctctccaac aattcaatga attagaaagc
     1381 agttattcca aaaccatgtt tagattatta aaacaataca gaaccaaagg aattgccaat
     1441 ttttcaaaag aagactttca tgaattactt gatatcccta aaagttatag agaaagtgat
     1501 ataaataaaa aaattataaa gcctattcgc caagaactaa ccgcaatctt caaaggtttg
     1561 acaatccaaa agagatacgg aaaaggacgg ggaaagcctg tcattggcta tcaatttaca
     1621 tttaaacctg agataaaaaa atcagacgac tttaacaaac atgctccaac caaagaagaa
     1681 caaaataaag ctttggaact agctaatcaa caggttcaat ctgaaaaaga acctacttca
     1741 ttacaagaaa aaatcaaaga acaaaaacga aaattagaac ttttacaaca gttagcagaa
     1801 ttagaaagaa aacaaaatac cccaagtaag aaaattaaca aacaaatagc agataacaat
     1861 tccgatatta ccccagaatt taaagaa
//
LOCUS       DAYYGS010000189         1859 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000189 DAYYGS010000000
VERSION     DAYYGS010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.197"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA44_002860"
     CDS             416..1804
                     /gene="ltrA"
                     /locus_tag="ACHA44_002860"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFM8906069.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
BASE COUNT          654 a          343 c          444 g          418 t
ORIGIN      
        1 aacttggtgg tgaaagtcca ctacaggctt ggcagtagga actgttagcg aaagacaagg
       61 cggcaatcgt gaggttgtgg ctgaaggaag tcggacgcaa actcctgaac tgacgaacag
      121 aaactagata caaggctgaa ttaggtcgga taagtcttca tcacaagaca aagtccaata
      181 ctgcccgaaa cctatacagt aaatctagca gttacatggg aggaaagttt tcactcttac
      241 cgggggagat ctcatgaggg tgttgtgggc gttgaatgaa aacaaaccag cacaacgatc
      301 atggcgacat gatggtgaat catgagaagt cagccgaggt catagtagta acgacactga
      361 ccgttacgaa ggactgaaca ataagaatct tgaataattt aggaggtgtg agaagatgcg
      421 acgaccgcag aaaacaagaa aacttggcta tcatcaaaga gctagtgtgg aacacgaagg
      481 gtatgatgaa gtgcgcagca cttcttgcgg tgaacagagg aaacaagatg gtgtagaaaa
      541 tctgtttggg agaattctag atcggaataa tctcaatcaa gcgtatcttc aagtagtcag
      601 aaataaaggg gcggctggtg cagatgggat gaccgtcgat caactgcttc cttacttgaa
      661 agagcaccgc gaggaattac tatccaattt acgccaaggg aactataaac ctcagcctgt
      721 tttgcgagta gaaataccga aaccaaacgg cggtgtaaga aaactaggaa ttcccacggt
      781 tattgatcgt atgcttcaac aggcaatcaa tcaagtgtta caaccgctat ttgaaccaga
      841 attctcagat aatagttatg ggtttcgccc aaatcgcagc gcacatgacg cagtgaagaa
      901 agcaaaatct tattatgaac aaggttaccg atatgtggtg gacattgata tgaaagccta
      961 ctttgacacc gtaaatcacg ataagctgat gtatttcatt gagcggaaag taaaggacaa
     1021 acaagtccta aaactgatcc gtcaatactt aatgagtggt gtaatggaaa atggactcat
     1081 cacaacaact gaagaaggaa cgccccaagg aggcaatctc tctcccctgt taagtaacat
     1141 ctacctgaac gaatttgatc aagtgctaga aaatctagga cataaatttg ttcgatacgc
     1201 cgatgactgc aatatctatg ttagaagcaa acgagcagga cagcgtgtga tgaagaaatc
     1261 cattcaattt ctcgaagaac aactaaagtt gacggtaaat cgagaaaaga gtgcggtagg
     1321 aagtccattg aaacgaaagt ttcttggatt ctgcattctg ccaacaaaga aaggtattaa
     1381 aattcgccct cacgcaaaag caaaacagcg tgtgaaaagc aagctcaaac aactcactaa
     1441 aagaaaccgt ggaagaagta tccaacttat tttaaaagaa atcaagcaac taatgactgg
     1501 ttggatcaac tactatggaa ttggtgagat gaaagaattc atgagggagt taaacggttg
     1561 gttgaagcga agaatcagac aatatatttg gaaacaatgg aagaatccaa gaaccaaaag
     1621 aaaaaatctg attcgtttag ggatcgataa agcgaaagcg tatgaatggt caaatacaag
     1681 aaaaggtatt tggtcagtct ccaaaagtca tattcttcat cgatcattaa cagacaaaga
     1741 actggcccgc caaggatatg aggacatctc cttgaagtac cagttcgtac actcaactta
     1801 ttgaaccgcc gtatacgggt ccgtacgtac ggtggtgtga gaggggcgga aggtcagtt
//
LOCUS       DAYYGS010000190         1845 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000190 DAYYGS010000000
VERSION     DAYYGS010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.198"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(66..464)
                     /locus_tag="ACHA44_002861"
     CDS             complement(66..464)
                     /locus_tag="ACHA44_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFM8906070.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(461..1234)
                     /locus_tag="ACHA44_002862"
     CDS             complement(461..1234)
                     /locus_tag="ACHA44_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8906071.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1231..>1845)
                     /locus_tag="ACHA44_002863"
     CDS             complement(1231..>1845)
                     /locus_tag="ACHA44_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HFM8906072.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
BASE COUNT          572 a          468 c          288 g          517 t
ORIGIN      
        1 caaaaaagcg gaaataacgc tcatccgcct tcttgtttgt gtttatttcc tcttcaaacg
       61 cttgtctatg attcagactt ttctctatcg ttattttcat ctaaaagttc ttgcattccg
      121 ataatttctc gatattgatg aatcttagtg ataccaatga ctttgtctac atcaatacta
      181 aaacaaatac cattacctgg aatatcgatg ttgatgcctt gaccaatcgc atccattact
      241 ttattcgtca aactatcggg tactaatgtc aacacaatat ctttttctgg cgaaatgtcc
      301 aaacctaaaa atttccctga atcatgtaca ccagaacctc ttccatgtaa gatcgttccg
      361 cctgctgcac ctgcttcttt tgaaaagtcg ataacatctt cgcccattcc tccgtcaacg
      421 atcgtcacaa tcatgtgaag attcaatgct acaagatctt tcatttgctt tcccccaatt
      481 tttgcaatct ttctttttcg attctgtctt tttcatcttt tcgtttataa atatatccta
      541 gtactaaagt ggataatatt ggtgtcaatg caatcattgc aaccacacca aacccgtcaa
      601 tgatcggatc tcttccttca atttgtgcag cgattgccac gctcattgat aaaataaatg
      661 tcgaacacat cgggcccgtt gctactccac cgttatcaaa agccattgct aaaaagatct
      721 tgtccacttt tcgtcctaaa ataaatgcta gcccgtatcc tgggatcaag aaataataaa
      781 gagagaatcc gacgagcaaa cgccacatag acaatcctac tgctgcgccg atcccaattg
      841 aaattaccat cagcatgatt ttcgatcgga ttctcccctc gctcaattct tcaacctgtt
      901 tgatcattac atgaatcgcc ggctcagcaa accctactaa gaaccccatt aaaaagccaa
      961 ggggaaccag tatataattg taaggcaatc ccgcaatagc actgcctaaa tgctgtccta
     1021 ccggtacata agccatattg actccgtgca ggaaaaggat caacccaata gtcgttatca
     1081 agaaaccttt cataatgtca aagaaacgct ttttgcgcat acgaaaacta atgatattca
     1141 ttaaaacaaa taggattacg ataggtgcca ctgccaaaat gacttccatg gtaacgattt
     1201 cgattccatc tatcacttgc tgccagaaac tcattggaag atcaccccca aaatcatcac
     1261 ggcaagaatc ggccctaacg aagcaatacc gaccattcca aaactgtcat tttcattttt
     1321 cttttgtttg atcatactag tcatccctcc ggcaagggcc aagataaatg gaacagttac
     1381 tggaccagta gtcactcccc ccgaatcaaa agcaatcggc ataaattcgg aactggtgaa
     1441 aaatgaggcg atcaaaacgc ctatgtagcc tatagccatc aattgataat aggatagctt
     1501 gaatacgacc cgaagcagcg caaaggctag aaatactcca gtgccgacgc tgacgatccc
     1561 aatcagcaac actctgccaa tctctccgtc agatatatca tatacttgtt gtcccaaaac
     1621 ttgcacggaa ggttcagcaa tcgtgatcac gatcccgata gcaaatccca aactgaccaa
     1681 aacggtcaaa cttttctttt tgatcatata tttccctact agatctccga cttccatcat
     1741 agaataatct gctccaaaca aaaagagcgt cattccgatc atcatgattg ccgcaccaac
     1801 cagaaatgaa gcgaggtctt ctccttctaa cggtgcaaaa acaaa
//
LOCUS       DAYYGS010000191         1823 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000191 DAYYGS010000000
VERSION     DAYYGS010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.199"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1586..>1823
                     /locus_tag="ACHA44_002864"
     CDS             1586..>1823
                     /locus_tag="ACHA44_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906073.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEID"
BASE COUNT          770 a          213 c          306 g          534 t
ORIGIN      
        1 aaacaacttg aaatattgtt atctagaaaa aagtgaagag ataatccatg aattaaatgg
       61 cttttaaaaa ttgtatttga catgaattta cgacaaggaa ttagtgggga ttaagcgatt
      121 attctcttcc actttagaga aataatgggt taaaaagcca gaatacgtag agcaatattg
      181 acaacctata aaggagctat tgttgaaaat tgaagtaaca aaaagataaa gggaggttta
      241 catctatcag attaaaagtg atagtacacc catgaaacct gcaaaagagt tgaacctaat
      301 gtatatcggt ttaactcttt ttatttgcaa attaaaaaaa tatcaccgaa gtgacgaatg
      361 aaactatgac caaattaagc aagaaaatat atcttgcaaa taaagtagta taaaaagata
      421 ttgataaagc caatcaaaac tttgaatttc aaacgttaga taatcaaaca gatacaaaaa
      481 atgaaatcag ttctacgcgt acgattatta aaaataataa attttcacta gaagaaaatg
      541 gaaataaaat caaataatta ggaagaaaaa acaaagcaat tagaaaagcc tgcaagcaaa
      601 tttgagtaat cgagatccaa tttttttaag gaagtttaac aaaaatatgt aattctatta
      661 tcactattaa agagaaagtt ggagcggaat ataaagaaaa gagaaaagta attaaacgca
      721 aaaaaatgta gaacttaaaa aatataaaga ggaacaaccg ttaaaattag gaatgatgtt
      781 gtgtaaacat tataattgct atgatataaa tgaagtgtta aagaaagcaa atttaaacca
      841 attttgtgaa cagaaaagcc ttagtcacta tgaccaaaac tacggacgaa actatgacca
      901 aaagtaggta ctaaattatg accgaaaata ggtccaaaat ggattttttt atgatatcaa
      961 aaattatgaa atgattttga aagaaaacca gttggcacag ccaacttagc caccttcctg
     1021 taggaaggtg ctatcagttt tcccttatgc tcttttcttt cttttttgtt tcatatgtca
     1081 aatgccattt tgaaatgtac catcttaaga aatttatcct taaaaacaat tgcgttattg
     1141 gataaaaaga tgctacatta tcttaaaaaa ggaatcaaat atgatgaatt tagtggctag
     1201 catatcgtgt gattaagata ctttatagtg taagttgaag gagaaaaaat atttaaaata
     1261 tacttcccat ttcagtggga agtaaaatta atcttgttgt ttctactagt aatgtatctt
     1321 tttggaaact atgtaacgat gtaaacaaga tttttttcag aagaaaaaac gataaaagtt
     1381 gtgataaacg atttaaaaaa taggagaaaa tatatcaagc gcatggtatg aaacgcacat
     1441 ttaaaaagat tcgactaact aaaaaaataa gtttacaaag cattgttgtg tattacaagt
     1501 gatctataca ctgaaaaatg atagagaaaa tctgatacgt ccgcaacaat ggacaattga
     1561 aaatgaaagt gaggaaattg gaagaatggc tgatgaaaat aaagttgttc cgcttgaaac
     1621 actagtgata aataaggaag agtttgagca actttttctt gatttttttg ttcaatatga
     1681 caggatttat gattttagtt cgtcactaga tttatttgaa ttagaggcag aaataataga
     1741 aaacagtcga gaggagaatg gtaaaatcat catttcagta gaaaaattca atgaattatt
     1801 ctcagaaaca tttgaaatag ata
//
LOCUS       DAYYGS010000192         1781 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000192 DAYYGS010000000
VERSION     DAYYGS010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.200"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(348..1489)
                     /locus_tag="ACHA44_002865"
     CDS             complement(join(348..1261,1261..1489))
                     /locus_tag="ACHA44_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8906074.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          483 a          344 c          334 g          620 t
ORIGIN      
        1 tcgtcatctt gtaaattact gacaaaaaac ctactatcac ttctcataat tatattctcc
       61 taatatgatt ttttcaaaat aatcaatatg taacatagaa gtatactcta ttgttacata
      121 ccttatttct tattatatca gtatttctct gtgacacata agggtgtaac atggatttga
      181 gtcaatacct cggacacgat ttgtgagaat tttttcgaga ttccagcggg tgagtatctc
      241 atcgtttcga ccagcggtca aaccaaaacc gtttgaccgc tgatcgaagc gatgaagttg
      301 aaaccctgtg gaaatctcgc caaaaaaatg taagtggtag ctaggaacta agcttgcaat
      361 gccaactcct ccatctgatt tggtgttaaa tagttgatac tactatggat tcgcttgcga
      421 ttgtaaaatc cttcaatgta ttgaaataac gctaaataag cagcttcaaa gttttcatac
      481 gcttttgatg gatagacttc ttcttttttg agtgaggcgt gaaacgattc gataccggca
      541 ttatcgtagg gacagccctt acgactgaag gagtgccgaa tgttcaattc attcagcttt
      601 gcctcatagg cttcgctagt gtactgagaa cccaaatctg agtggatgat caagttctcg
      661 gagggtgttt ggtttaagag cgcattgtca agggtttcga taaccaattc tttcgtcatc
      721 tgcttgccaa acttccaacc gatgactttc tttgtgtgga ggtcttgaat agtggagagg
      781 taggtccagc catcttttag ggtatgaata taggtaatat ctgtcaccca tttttgattg
      841 atcgacgtag tggtaaaatc ttggtttaac aggtttttac gtccttcact tttaactttc
      901 gcttgtttgc caggcttgta cttcttgagg gtgactgaac gcaagcctaa ttttctcatc
      961 agtatttgta ctttctttaa ggacaaggtc tcgccacggc caacaagaat cttatgaatt
     1021 ttaggcgcac catagatacc cttgttttca aagtagattc ctgaaatcaa gcggtttaag
     1081 cgtttgtttt ctgattggcg cttggaaggt ttctttttct tccagtcata gtagctggat
     1141 ttcggtgctt ccaaaacggt acacatttgt tttacgtcat gatcatgaac atttgattga
     1201 ataaattcga agacatcact catttttact tttgagcgaa tatggttaaa gcctttttta
     1261 ggatagtgtt ttcctcctgc atgcgggcca tttccttttt catcgctagg atttctgcct
     1321 cagtcatgcc ggttgctaca ttctttgtat gaagcttctt ccaacgataa atcgtttgtg
     1381 tagcgatacc atattgcgct gccagttctt cgaccgattg gccagactgg tttaaatcta
     1441 agatcatctt cttaaagtct tcatcaaatt ttttcttggc ttttgccatc gtggacacgt
     1501 ccttttctta gtagttagat tctaccaaat cgtgtccatg aaatcatcct aacatcaaca
     1561 taattatttt aaacgaaatg actacaagta gtcattgaag caaatctcca taagacattt
     1621 gtaaaatctt ttaaaaagaa gcgtcgaaag aaggaacttt tcttcaacgc ttcttttatt
     1681 gactcaattt tcaatattac ttgaacgaat aatctgattt ctaaactctc gaaaaacttc
     1741 ttgtggttga taatagtctg gatctatttc aaatgtttct g
//
LOCUS       DAYYGS010000193         1768 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000193 DAYYGS010000000
VERSION     DAYYGS010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.201"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA44_002866"
     CDS             complement(140..1288)
                     /gene="tnpB"
                     /locus_tag="ACHA44_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="HFM8906075.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHA44_002867"
     CDS             complement(1305..1709)
                     /gene="tnpA"
                     /locus_tag="ACHA44_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HFM8906076.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          529 a          304 c          280 g          655 t
ORIGIN      
        1 taaacctgac ggttcttaac gcttgaagtg ggagcttctt gggaatagag catacttgat
       61 agcgtatttc tttactaaca gcggtgtctc ttatttacca agctatcccc gtagttccta
      121 cggttcttga tgattggatc taagccaatc ccatagaatg tagtcttaga ccttcggtca
      181 gaatattgat actagcattg atatcacgat catgatgagt atgacaaaca ggacaagtcc
      241 attctcgaat ttcaagagat ttcttgccat ctttatgtcc gcactctgaa caaatctgac
      301 tagacggaaa ccatttatct actttgatga tttttcgtcc ataccagtca gccttgtatt
      361 gtaatttagt cacaaacttt gaccaagaaa catcagaaat acttttagct aatttatgat
      421 ttcgcaacat accttttgtg tttaagtctt caatacagat aatatcgtga tttttgatca
      481 tttctgtact caacttattc agaaaatcag tacgttgatt cattactttt tcatgcaatc
      541 tagctacttt gcgtttttgt ttttgataat ttttagcttc aaatagattg gtacctttat
      601 ttttagccaa taaagcacgc ctagacaatt tgcgttgttc acgctttagt ttcttttcca
      661 ttttggacgt gaatttatta ttatcaattt tttgtccatc agaaagaatc gcaaagtcca
      721 taatacctaa gtcaatacca attgtggaat tagttttagg taattcgata atttcttctt
      781 tacacaacaa agaaatatag tatttaccgc tagaatgacg tgatattgta gcagatttga
      841 taatcccttt tggttgtcta tgaagcttaa ttcttactaa tgacttcaat ttaggaactt
      901 tgatgaattt actatcaatc aaagcaactg taccattttg attattcgtt gtataactct
      961 gaacaggatt tttcttactt ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat
     1021 tcttgtatgc tttatctaaa ttaagttggg cattggctaa agcaaggcta tcaatttctt
     1081 tcaaaaacgg aaattctttc ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag
     1141 aatcattttt aacttcttca tacgcctttt ttcggtcatc aagcattaga ttgtagacct
     1201 tacggacaca accaaaagat ttggcaaaga agatttcttg ttcctctgtt ggatagattc
     1261 tgaatttgta tgcttttagt tgttccataa agttcaccta ctttctattt atgattgttt
     1321 ccttgatttt ggatatactt cttaatcaca tcaattggtg cgccaccagt tgtcagaaga
     1381 caaaaacttt tagaccagaa catttctttc caaagaaatt gtttgactct tggaaagtca
     1441 cgttttatca atcttgagct ggcactttta taggcattga tgaattttgt caattctgtt
     1501 ttaggttgag ctttgaacat aatatgaacg tggtctttat catgattcca ctccactaaa
     1561 gtaatgtgat acgattctga aattctttca aaagtagttt tggcataatc agatatttca
     1621 tcatcaatca cttgtctacg atatttcgtt actaaaacaa ggtggtaata aagaagaaat
     1681 actgaatgat tatttgtatc taattccatg ttatatcaac tcatttcttg tatagactga
     1741 ttgtaacatg gaacaaaata aaaaggca
//
LOCUS       DAYYGS010000194         1735 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000194 DAYYGS010000000
VERSION     DAYYGS010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.202"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(147..433)
                     /locus_tag="ACHA44_002868"
                     /pseudo
     CDS             complement(147..433)
                     /locus_tag="ACHA44_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(423..752)
                     /locus_tag="ACHA44_002869"
     CDS             complement(423..752)
                     /locus_tag="ACHA44_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFM8906077.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(829..1101)
                     /locus_tag="ACHA44_002870"
     CDS             complement(829..1101)
                     /locus_tag="ACHA44_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HFM8906078.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(1101..1364)
                     /locus_tag="ACHA44_002871"
     CDS             complement(1101..1364)
                     /locus_tag="ACHA44_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFM8906079.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            1598..>1735
                     /locus_tag="ACHA44_002872"
     CDS             1598..>1735
                     /locus_tag="ACHA44_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HFM8906080.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT          558 a          333 c          222 g          622 t
ORIGIN      
        1 gaaatatatt gatttatagc tttcctcaaa gtaatgacgt tatttaattt atcgagaagc
       61 agagaatact cgcgacttta gtcatgtaga cgaatcagat tttcgataaa aaagaagccg
      121 tccttcttaa gaacgacaac aaatcactag attaattttt tcaactgttc ataaaagttt
      181 tcatgcgttc cacataacaa tactggaata accttgtttt ctataatctc gtaagctact
      241 ctatatgttg tgcccgcata tttgaaccca ctagaccaaa tacctgataa atccccagtt
      301 ttttgttcac ccttatatgg atcaatcgca atttcatcat aaattaaatt caaaaatttt
      361 tctttcaatg gcttctcttt tagcttttta aatacttaat gacatgtttt gaaggaacga
      421 tttcataagc caatttctgt tgccaactcc tctttagtca ttactttaga attagtaagt
      481 gtatcttcag caatatctct aaaggaagaa tgcattttgt tttttctatt cttaaattct
      541 acaagaatct catctttatc gtagccttct gcaattatat cagacaaaat atcttcgcta
      601 aaatcataaa attctttttg tggttcaaca aattcataaa gaataccatt ctctaccaac
      661 tttgcttcaa cttcgactcc attaggaaca ttaaaatctt taggtactgt caacatgatt
      721 gaattgcctt gttttcttgt ttttgtaatc atttaaatcc ctcatttcta acagtatgta
      781 attacttagt aaatacatta taccacgaga taatgtattt acaagtaatt actgtgactc
      841 gtaatgcgtc cttgctgaat aaatttcaac cacgtgttca tcttcatcta ccttataaac
      901 aacccggtgt tgtctattca atcttctaga atacctacct tcatgtgtag gttttaactt
      961 ctcaaatgat tgggtaggga gatagggatc ttcctttaaa gtctgaataa ccttctcaaa
     1021 ttgaggtttc aaattagact gtttgatttt ctttaaatca gctttagccg agtttttaat
     1081 catcacagaa tagttattca ttataagtta tcccaatcaa tttcatcaac attagtaaat
     1141 ccactatcat ctttttctcg cttacgaaca acacccaaag tgcctatttg ctcaagtaat
     1201 agcgtttctt tgattgcttt ccaatcatcc aaaccaataa gcaccgcatt attttcactg
     1261 cgctcactaa tgatttcaat ttccgtatga ttctcattta catctttcaa caacttataa
     1321 aaatttttcc gtgctgagct aggatttaag actgtttttg ccatatctac tccccctcta
     1381 atttatatct ccattctaac gtacttaatt aagtacgtaa attcgattgt tttgtttaaa
     1441 ttggagtacc aaaacagcat tgtaaagtgt tatccgtttc ctttataccc gtggtgctag
     1501 ttaatttttt cctagaggaa aatgtctaaa catgttggta aatcaacaaa aaaacgcata
     1561 gagtagtctc aaaactccca agaaatgagg actacatatg cgttcatcaa aatatactga
     1621 acattataca gatactttta taacattcaa ggaaattatg agaactgttt ctaacatata
     1681 ccacaattgt gtaccagata agattaaaaa ccgaagaaat actgatcaac tgaag
//
LOCUS       DAYYGS010000195         1733 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000195 DAYYGS010000000
VERSION     DAYYGS010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.203"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..233
                     /locus_tag="ACHA44_002873"
     CDS             <1..233
                     /locus_tag="ACHA44_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8906081.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
     gene            392..1687
                     /locus_tag="ACHA44_002874"
     CDS             392..1687
                     /locus_tag="ACHA44_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8906082.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          562 a          335 c          313 g          523 t
ORIGIN      
        1 tattaaaaaa tcttcctgaa acacttgatg cacaacttcg cacgaaactt caaaaccttt
       61 taacgtatga agaaggtatt tacaatgcta tgatttaccc gtattctaat ggcaaaatag
      121 aggccaagat tccccatatc aagacactga aacgattatc ttatggattt aaatcatttg
      181 agaacatgaa aatcaggatt tttttgatca atcaattgat tcaagtgaaa taacaaaaaa
      241 atctgagaca gaaggtcagt ccttctatcg gctctatact ttttgatgtt ggtgaacttc
      301 accaacatct tttttgtata aaaaaatata gaaaagacac cctgaaatcc tttagaatta
      361 atttggctac caattcatag gaggattcaa gatgtctcat actcagctta tcaaagatac
      421 cctaaatatt ctagacctaa atatctattt cgaggaaaat tgtttaacta aagaaaaata
      481 caaagggcaa atttgcttga tttacaaagg aactcttctg tacaagccag aagagtgttc
      541 ccattgtctt tgtgtcgtcc cctctaggat cattcgttgg gggacgacaa ccgttcggtt
      601 gttactgaat gatgtgtctg agtatcgcac ttatctggag ctgaagaaac aacgatttaa
      661 atgtaagtct tgccaaagaa cattcgtagc ggatacctct gttgctgaaa aacactgttt
      721 cattagtcaa aaagttcgtt ggtctgtcat tgctcgctta aaagagaata cttccatgac
      781 ggaaatcgct cgtcagaaaa atatttccac ttcttcggtc tatcgtgtca tgaagcgatt
      841 ctaccgccca ttgaatcctt tcaaacagac tttgccaaag gtcttatgct ttgatgaatt
      901 caaatcggtt cgaagtgttg ccagtgcaat gagttttatc atgatggacg gacaaagcca
      961 tgcattattg gatattgtcg aaaatcgacg cttgccttat ttagaacgct atttttctcg
     1021 gttttcatta gcgactcgtg aagccgttca attgattgtt attgatatgt atgcgcctta
     1081 tgtttcgttg gtgaaaaagc ttttcccgaa tgctcagttg atcatcgatc gtttccacat
     1141 cgtccaacat attggcagaa ctttcttgaa tcatcgagtg aaagaaacca cggtacgact
     1201 taaaggaaat tcccactctc aacgagggtt agggaaaaaa ttgaagcgct attggaaact
     1261 cctacaaaaa gaagaagcaa agttaaacta tgaaaaacgg gtttggcgtg ccagttttaa
     1321 agattatttg actgaaagtg agattgccga tcgactgtta gctgcttgtc ccaacttacg
     1381 ccaaggctat cagttatacc aagacattct ttatgcggta aagaaaagag atcagtcact
     1441 atttgaggat tgtttaacta gagacattag cggtctgcca gaaacctata caaccacttt
     1501 acgaacgttt aaaaagtttc tgccacagat ccaaaatgcg cttcactaca gctattcaaa
     1561 cggaccattg gaatgcttaa acaaccacat caaagtatta aaacgcaatg cgtacggctt
     1621 tcgcaacttt tacaatttca aactacgaat tatgattcgt catggcaaaa catttttaac
     1681 caaataaaca gaacagaaag aggttttctt ctttctgttc tgtccaaatt att
//
LOCUS       DAYYGS010000196         1705 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000196 DAYYGS010000000
VERSION     DAYYGS010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.204"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1135
                     /locus_tag="ACHA44_002875"
     CDS             <1..1135
                     /locus_tag="ACHA44_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="HFM8906083.1"
                     /translation="KKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQAIN
                     FAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIK
                     YIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWK
                     SPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWR
                     IAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQII
                     KYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQG
                     GYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1108..1551
                     /locus_tag="ACHA44_002876"
     CDS             1108..1551
                     /locus_tag="ACHA44_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906084.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLG
                     TWQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNL
                     LTLRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
BASE COUNT          639 a          273 c          265 g          528 t
ORIGIN      
        1 aaagaaaatt tatctaggtc tcctcttttc cttgatgatt ggaattttta gtataggaat
       61 tacaaatacc tatgcttcag aaaattctaa tttcaatatc aactcccaac caattgaaga
      121 acaagcaatt aattttgcga aaactgattt tgaacactcc ataacttcaa ttcttttaat
      181 ggaaaataac accgaaactg atattaacta tactcttggt tcgccattta aaatgctcga
      241 tggagaaaat acggcaaata gttctatttt ctttccagta ttttcagacg ctgataatac
      301 gattaaatat atttataccg tcaaaaaaaa tacaaatgga gaatattcag gaagtatctc
      361 acaattttta gcaaaagaac ttcaaaatct agtagatagt aatataacag atacaacctt
      421 attttttcaa gataatagca tttattattc cgataatgga caaattaaac tactttggaa
      481 atcaccttta agtacagaaa aaaatggtgt ttcttccttt acagaaaaaa atgatcttat
      541 atccgtaaat ataaaaaata aaattaaaaa tttctctaaa aaacaaaatt ttcctgcggt
      601 taaacgtgta aaaagtgtct cctccggaga taactttatt atgattaatt ggcgtatagc
      661 agaaacccaa acaagttttc cgtggtgtgc tgcttacgta gaagctgcta tcttatgtaa
      721 caaaacagat gtacgtgcta cttcagctag agccattatg cattatacct atcctaattt
      781 aaacaacagt caaattgaat caaaaagtct taatcaagat caaataataa aatatgcaaa
      841 ttactgtggt gtttatccta aaaaagtagc acgaatacta aacctttcag aggtccaaac
      901 acaactaaga aaaggaaatg ccatttatat gggaactgct ggttatggaa attatagtaa
      961 cagccgtcat gcacttgcat tatttgggtg ggtcaatcaa ggtggttatc aaacttacta
     1021 tgtgtggaat ccttggtata actacccaac tgtaataagt gcaaatgcag gaccagttac
     1081 aattcctgta cctggaggtg gttttacttg ggacaatacc attacaaatt ggtaaataaa
     1141 cccattgctt tagttatggg tctagtgtta gttcttttct taactagttg ttcaaaaaaa
     1201 caagaaatac aaaagactga tctgattcct tttctaggaa cttggcaaac taaagatcat
     1261 tcagaacgtc tagtaatcac taatttaaat acttcaaaaa atatagaaca atataccatc
     1321 ttagtaaaag agaacaatca taaaattcaa atcattgata aaaatttaaa tataaatgaa
     1381 caagttttca tttacaaagc aacaaataat ttactaacac tgcgctcgaa taaaaaacaa
     1441 ttagattaca cttttagttt ggattctcca aaaaagctcg tatttatttt gacaacgaat
     1501 gatgggacag aagggacagc taaaccaatt gaatttttta aagctgattg atgaatagtt
     1561 gtttgatatt acaaaacaat aatttcaccg aaggtgaaac aagcgaaact ttacaaaaag
     1621 tggctagcat ttgctagcct tttttgtatt gtggagcgtg ggttttgagg gggattcaag
     1681 ggggagcttg cgcccctttg acaag
//
LOCUS       DAYYGS010000197         1678 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000197 DAYYGS010000000
VERSION     DAYYGS010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.205"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..765)
                     /locus_tag="ACHA44_002877"
     CDS             complement(112..765)
                     /locus_tag="ACHA44_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFM8906085.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1053..>1678
                     /locus_tag="ACHA44_002878"
     CDS             1053..>1678
                     /locus_tag="ACHA44_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFM8906086.1"
                     /translation="MDNHTRKLLVLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
BASE COUNT          542 a          305 c          232 g          599 t
ORIGIN      
        1 ccaaatatgt tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc
       61 caagaacaga aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct
      121 ctccaattta tatcctacat ttcttatagt agttatacag tctaattgta atttttttct
      181 aagttctttt atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca
      241 tacataatca ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt
      301 taatacttct agttcttttg catttacatc tatttcttca ccatttattt tagctgtata
      361 gctattaaaa ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt
      421 cttaattaaa gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata
      481 tccgtctact aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa
      541 gattagaatt ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat
      601 aaaaggtatc tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc
      661 ttctcttcca tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat
      721 tccctctctt atcatattat catcttcaac agttaatatt ttcatatata cctccactta
      781 agcctataca ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac
      841 accttctgtt gccaatactt tttctttttt ctaataaatg caaacagaca ccttgaaatc
      901 ctttagaata aagggttcta taatattttg tgttgttgga aaatcgaagg cgattttcta
      961 ctaacacttt ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga
     1021 ccaaaacatc accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg
     1081 tattaacaga taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag
     1141 gcgttcaagc gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact
     1201 gtggatcgat tcgtattatc cgcaacggtt cctatacgac acgtactcag atattgaaag
     1261 tcaaagaaaa gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg
     1321 ggcagacctt ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac
     1381 tgaagcaggc gattctcata gaactgtacg aaaatcaatc acgtaaacta atcgctaaga
     1441 agtacttcgt ctcggatggg acggtgacac ggatcttgcg tgaagcaacg aagcattacc
     1501 agccacgtat gaacttcctt ccgacggtgt tgtgtatgga tgagttcaaa tcaatgaagt
     1561 ctgtttctgg ttccatgagt tttatttgtg ttgatgggac aacccatcag ctatttacga
     1621 tccttgaaga tcgcagactt tacaaactaa ctcaatactt cctgcgcttt cctagaaa
//
LOCUS       DAYYGS010000198         1642 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000198 DAYYGS010000000
VERSION     DAYYGS010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.206"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1594
                     /locus_tag="ACHA44_002879"
     CDS             128..1594
                     /locus_tag="ACHA44_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HFM8906087.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          460 a          296 c          337 g          549 t
ORIGIN      
        1 attgccatta aaaattactt gcttttatag aaaattagtt tatatttctt aaataagttt
       61 tttagttaaa tttttcggta aagatgtata atattttctg aaaatttaaa gaattggggt
      121 atagattatg gaaaaacagc aagacaaggg cttcttcggg cagccgaagg gattgtcaac
      181 tttgttcttc actgagatgt gggaacgatt tagttattac ggtatgcgtg cattacttat
      241 ctattatatg tacgatacag ttgccaacgg gggacttgga ttaccacgag caacagcatt
      301 agctatcatg tcgatctacg gatctttgat ctacatgtct agtatcgtcg gcggatgggt
      361 atctgaccga gtgttaggag caaaacgcac tgtattcttc ggtggagttt tcattatgct
      421 tggccacatc gtcttggctt taccatttgg tgtaagtaca ttgtttgttt cgatggcatt
      481 gattattatc ggaaccgcct ttttgaaacc aaatgtttct ggcatggtcg gacatcttta
      541 ttcaaagact gacttgcgaa gagatgcagg attttcgatt ttttacatgg gtgtcaactt
      601 aggggcatta ttagcaccac taatcgttgg tacaatcggt caagaatata actatcattt
      661 agggttctct ttagctgcta ttgggatgtt ctttggatta ttacaataca tcatccaagg
      721 acgcaagaca ttagacggag tgggtacaac accgccgaat cctttgacaa ctgaagaacg
      781 taaaggattc atccgtaact tgatcatcgc acttgttgtt atcgctgcag tattcggcgg
      841 ggcacaacta actggtcatc tgacaattga cttttttgtc aatacaatca gtgtattggg
      901 gattatctta ccgttgtatt actttatcaa aatgctgact tcaaaagatg taactgctga
      961 agaaaaacca agagtatggg cttatatccc attattcctt gcagcaatca ttttctggtc
     1021 attacaagaa caaggttcat ctatcttagc tttatttgct agtgaacgta cacaatctac
     1081 gctatttggt gtaacgatcc aagcaagctg gtatcaatca ttgaatccaa ttttcatctt
     1141 tattctgaca ccagtattcg taacgatgtg gacgaaactt ggtgaacgtc aaccatctac
     1201 agtagtgaaa ttctctcttg gacttttatt tgcaggatta tcgtacttgc tattgatgtt
     1261 gccgggtatg ctttatggta caagtggaaa agtaagtcca ttgtggttgt tgggaagttt
     1321 cttcatcgtg gttattgctg aattgtgtct ttcaccagtt ggtttgtctg tgacaacaaa
     1381 attagcacca cgagcatttg aatcacaaac aatcgctatt tggttcttag ctgatgcttc
     1441 ttcacaagca atcaatgcac aaattgcacg cttctacaca ccgggaacag aatctgctta
     1501 cttcggtatt atcggtgcag tagcaatcgt cggtggtatc ttgctattta ctgtgaaaga
     1561 accaatcaaa aaactaatgg gctctattca ttaatcaatg aacataaaaa caggaattgc
     1621 ttatttgcag cagttcctgt tt
//
LOCUS       DAYYGS010000199         1507 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000199 DAYYGS010000000
VERSION     DAYYGS010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.207"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(112..627)
                     /locus_tag="ACHA44_002880"
     CDS             complement(112..627)
                     /locus_tag="ACHA44_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HFM8906088.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(809..1420)
                     /locus_tag="ACHA44_002881"
     CDS             complement(809..1420)
                     /locus_tag="ACHA44_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFM8906089.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
BASE COUNT          522 a          302 c          196 g          487 t
ORIGIN      
        1 tctatatcta aatttagttt tatgtattcc tcatagaaat aaccgagcat ttgctcggtt
       61 attgttcttc ttattttcca gtatccttct tttctccaca gctcatttta atcaaaagct
      121 taatctttcc acatttttgt ctttctcact tataacgaaa cactgccgac tgaccggcca
      181 ttcaatatct acgctttcat aacttataca tttggcagca ttagaattga aatcattatc
      241 tacgcaccac tgaactagcg tattgtcctc catagtaaga tagccatgag catatccacg
      301 tgggatatac gcacatttac cattgctttc actcaatgtg ttgacataca cacatccata
      361 agtggaagat ccttgtcgaa tatcaagtgc gatattcata atttcaccat gtaggcacat
      421 cactatcttt ccttgagcat gctctccact ctgataatgt agccctcgca tagtatattt
      481 attatgacta aatccttgat ttatttgctt aatgtgaaac tcaataccaa tcgagaaaag
      541 agtctcatta tccaatgcaa cagcagtata tcctctgaaa tccgaaaact taattatatc
      601 aaacactttt accataacta ccaccatgtt atatatttta ccaatttttt aattcgctcg
      661 acctcattta aagcaataaa attccctacc tcttgaagat ataacaacaa tgttttagca
      721 aaaatagatg gcatgtaaag tattcttgat gagattacac aatcctgtct gcagaaaaat
      781 attaaccata ttaaaattca aagcagttct attttaagtc tatatactta tttaatcggt
      841 agaatagcca atatagcaaa gcaccaatta agccccctag ggtgttataa aacaaatccg
      901 atagttgaaa tgtaccaagc ctaaaaaaca gttgtgtaaa ttctatactt aatgagaata
      961 aaaacacata cttaatccca acatagatgg tgcgtataat atcacattta atgtcacgcc
     1021 accataacaa taacatgcaa aaagggataa acagcatcgt gttctccacg cattctgttg
     1081 tcaatctgct ctctcctgta gcattatcta taatatataa tgaccactgt ccaataacgt
     1141 ttgaaagcgg gttcatccac atatttcgat taagcaaggt tttaaataat atcaaagtaa
     1201 aataaaacgc aagaaaaaag agtctgcgaa atttactaga tgctttaaaa tatctcaacc
     1261 agattaatac agaatctttt aagtttttag gatctcgaga gttatcaact ataaatagat
     1321 accagtacat aaacagatac gctgttaata cggcaaataa gaacggctga tataatgctg
     1381 ttaatacatt taccgttata gcagaaataa tatctatcat tctctatctc tcaatgagca
     1441 aatgtttatt tgctcataat tctccttact aaaagtagca atcgttttct aaatataatt
     1501 gcagcga
//
LOCUS       DAYYGS010000200         1506 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000200 DAYYGS010000000
VERSION     DAYYGS010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.208"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..437)
                     /locus_tag="ACHA44_002882"
     CDS             complement(<1..437)
                     /locus_tag="ACHA44_002882"
                     /inference="COORDINATES: protein motif:HMM:NF025639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HFM8906090.1"
                     /translation="MFQILKLSKNEIRHSNFLAFLFAPNETHNLSDEFFKMFLKRYID
                     SNDDTKAAINYFDALLNSYEDLIVYRENKNIDILLVSEKNKIVVCIENKILSSESGGQ
                     LNKYQIYIERNYSNYKKIFVFLTPDGNEPTNPSWGIITYKNIV"
     gene            complement(401..526)
                     /locus_tag="ACHA44_002883"
     CDS             complement(401..526)
                     /locus_tag="ACHA44_002883"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906091.1"
                     /translation="MIDEKKCLKNLLEDMDDIEENYLNGLGKQIYFRYLSYLKMK"
     gene            complement(729..1208)
                     /gene="rlmH"
                     /locus_tag="ACHA44_002884"
     CDS             complement(729..1208)
                     /gene="rlmH"
                     /locus_tag="ACHA44_002884"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFM8906092.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
BASE COUNT          433 a          274 c          201 g          598 t
ORIGIN      
        1 tcaacgatat tcttgtaagt aattatgccc caactaggat ttgtgggttc atttccatct
       61 ggagttaaaa aaacaaaaat ttttttatag ttagaataat tgcgttctat atatatttgg
      121 tatttattaa gttgccctcc agactccgaa gacagaattt tattttcgat gcacaccact
      181 attttatttt tttccgatac caataaaata tcaatattct tattttctct gtaaacaatt
      241 aaatcttcat agctattaag taaagcatca aaataattga tagcagcttt ggtatcatca
      301 ttagaatcta tatacctttt caaaaacatt ttaaaaaact catcagataa attgtgggtt
      361 tcatttggag caaacaaaaa agctaaaaaa ttactatgtc ttatttcatt tttagatagc
      421 ttaagtatct gaaatatatt tgttttccca gtccatttag atagttttct tcaatatcat
      481 ccatatcctc taatagattt tttaaacact ttttttcatc tatcatgttt tttctccttg
      541 ccattattct aactaaaatt ttacgtattt gtttctatgt ctacattatc tttttaacag
      601 tatctatttt tcttttgcaa cttacttggc taccaaagca aataaaaaag ttatttataa
      661 atttctccct accactacct attgctatta tttaaaatag ggataatttt ttctcttaaa
      721 aaatatcatt atttgtggta tggagccccc gcattgatcc ggaaggcacg gtagatttgc
      781 tcgacaagga tcagacgcat caattgatgt ggatacgtca tttttccaaa agagatctga
      841 gcattgcttc ttttcatgac ggcttcgctg agtcctaaag agccgccgat aacaaaaaca
      901 aggtggcttt ttccttggat acctaattgg tcgatttgtt tagcaaaaga ttcacttgta
      961 ggatttttcc cttcaattgc taaggcatag acgtattctt gttctttgat tttcgctaag
     1021 atccgttccc cttctttttc ttttacttgt ctcatttgcg cttcgcttaa attctccggg
     1081 gctttttcgt caggaacttc gatcaattcg atttttgcat aggcattcag ccgtttgaca
     1141 tactcattga taccttggat caggtatttt tcttttaatt ttcctactga gataattttg
     1201 atattcatgt tttctctcct ttggtcttca tgctcacttt gtttattata atcaattttt
     1261 tcgtctatat gtttattttg gctatttttg ttaaaaagat ttccccaaca gttatgcaca
     1321 acatccacag gtttatccac agaaaacagt gttattttac cttatttcat cacattcatt
     1381 aggcaaacat acgttttata agaatttatc cactttatac acaggctgtg gatatgtttt
     1441 atcccttatt tttccaatcc tatccgcgta gttttacaca gtatctgtgg aaaaagaaaa
     1501 tcaaat
//
LOCUS       DAYYGS010000201         1480 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000201 DAYYGS010000000
VERSION     DAYYGS010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.209"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            64..972
                     /locus_tag="ACHA44_002885"
     CDS             64..972
                     /locus_tag="ACHA44_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HFM8906093.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKIKYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FDRWREEQENSFQDWREGQESDYLSWFESIKDILKSIDPGGVMLAELMDARVDLQGVR
                     HESISERFLSDLNYLYQKMKAALFTIEYGEIEVTDILQDDLFSDNHEVEKIETVEFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            972..>1480
                     /locus_tag="ACHA44_002886"
     CDS             972..>1480
                     /locus_tag="ACHA44_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906094.1"
                     /translation="MVKVKRMMETDESGVKRQFMPITHVSAVLGLEKMISGQSKVLSV
                     NGKYGAVILTKADLGLENAITELPYASETSDGILTAEMFQKIVNGEGGTYILPIATPE
                     QLGGIKVGELLEVTEEGVLSATKQTDFNFSEELKGKLESLKILKAGANISIAEDGTIS
                     STGGSGTGGV"
BASE COUNT          529 a          197 c          316 g          438 t
ORIGIN      
        1 cagtattata gtgcaagtgc aatttcttcc attgtcatac caccaaaata gggaggtgat
       61 ttaatggcaa agtggaatgt catattaagt acaacagaac cctataatta cgtggggatg
      121 attcaagttc gacaaggcaa taaaaacact gaagttatgg aagctacaat tgtcgaaaat
      181 ggtcttccat atgatttatc agaatgtaag gtatattttg aatcagttgt tggcggaaaa
      241 tatccagtcc aattagaaac aaaaattgtg gatgctaaaa aagggaaaat caaatatatt
      301 tttgataaat attccatgca gtgtttgcat cgacaaacag ccaatttcat tatatttaaa
      361 ggagaagact tgattggaac aactcaagac ttctcttatt ttgtcatcaa tgctgtttca
      421 aaaacagaag gagaaatggg ttcttattgg caatcaattg aagatttaat tgctgatatg
      481 acagatttta ttaatgaaaa taaaggcgat tttacggact ggatgaatga aaggaaagaa
      541 gaatttgatc gctggagaga ggaacaggag aatagtttcc aagattggag agaaggacaa
      601 gaatcagatt acttgtcatg gtttgaatca atcaaggata ttttgaagtc tatcgatcca
      661 ggtggagtaa tgttagccga attaatggat gcacgtgtag atcttcaagg tgtacgccat
      721 gaatcgattt ctgaacgttt cctatccgat ctaaattatt tgtatcaaaa aatgaaagca
      781 gcacttttca cgattgaata cggtgaaatt gaagtgactg atattcttca agacgatctc
      841 ttttcagata atcacgaagt cgaaaaaatt gaaacagtag aatttccaat tgaagaagga
      901 gctttgatta ttgcaactgt tgatgatcca aaacagaatg ttttcactct tgagaaagtt
      961 ggggtgattt gatggttaaa gtaaagagaa tgatggaaac tgatgaatct ggtgttaaac
     1021 gtcaatttat gccaattaca catgtctctg cagttttagg cttagaaaaa atgatatcag
     1081 gacaatcaaa agttctttcg gttaatggta aatatggtgc agtcatttta acgaaagctg
     1141 atttaggatt agagaatgca attactgaac taccttatgc aagcgagaca agcgatggta
     1201 ttcttactgc tgaaatgttt caaaagatcg taaatggaga aggtggaaca tatattttgc
     1261 cgattgctac tcctgaacaa ttaggaggta taaaagttgg ggaactctta gaagttactg
     1321 aagaaggtgt tttatctgca actaagcaaa cagacttcaa tttctctgaa gaattaaaag
     1381 gaaaattaga atctctaaaa atattaaaag caggtgcaaa tatatcaatt gcagaagatg
     1441 gaacaattag ttctacaggt ggtagtggga ctggtggtgt
//
LOCUS       DAYYGS010000202         1474 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000202 DAYYGS010000000
VERSION     DAYYGS010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.210"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            131..505
                     /locus_tag="ACHA44_002887"
     CDS             131..505
                     /locus_tag="ACHA44_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HFM8906095.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="ACHA44_002888"
     CDS             complement(619..1248)
                     /locus_tag="ACHA44_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFM8906096.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="ACHA44_002889"
     tRNA            complement(1382..1454)
                     /locus_tag="ACHA44_002889"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          498 a          225 c          258 g          493 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataa
//
LOCUS       DAYYGS010000203         1465 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000203 DAYYGS010000000
VERSION     DAYYGS010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.211"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            80..490
                     /locus_tag="ACHA44_002890"
     CDS             80..490
                     /locus_tag="ACHA44_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8906097.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            504..860
                     /locus_tag="ACHA44_002891"
     CDS             504..860
                     /locus_tag="ACHA44_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HFM8906098.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          541 a          209 c          271 g          444 t
ORIGIN      
        1 ttatatttta ttagctaata attggtattt taaaagaaat tactgtagaa ttggattata
       61 attaaaaagg agtgagaaaa tgataaaagt atatacttcg cctagctgta cttcctgtag
      121 aaaagcaaga gcatggttgc aagcgaatca acttgaattt gaagagaaga atatttttgc
      181 cgaaccgtta acagaaagtg agatcaaaaa gatcttggca gcgactgaag gaggagttca
      241 agatattatt tccactcggt cgaaaattta cgaaaagctg ggcatagatt ttaacgaatt
      301 aactttaaag caaatgatcg tcttattcaa agaatatcct tcattattga gacgacctat
      361 tttgattgat gaaaaaagaa tactaatcgg atttaatgaa gatgagatcc ggtcatttgt
      421 ccctcgtgaa attcgatgtg ttgccagaaa tctgatctat ttacaaaaca ataacatctt
      481 aaattcatag ggagggagcc ttcgtggaag tgaaagtcta tggatcagcg cactgtgtaa
      541 caagtctgaa ggcgaaacaa tggttagaac accatcaatt gccttatcaa tttgttgact
      601 tagaagaacg cgatttaagt acagaagaag cagaagaatt atgcagtttt gaagaagtgc
      661 aagcagaaca gctatttgcg acttggtctg aggcttttca caacattgcg attgaccttt
      721 cctgtgtagg aaaaaaacaa ctgatttttt tatgcacaca acataccaat ctcctcagac
      781 gtcctttgat tattatcgat gaagttctat ttattggata caatgagcag ttgatggaac
      841 agcttatctt aaataaatag actttaactg ttttaatcaa gtcactgagc atttcataca
      901 gttgtaacag tcgaacactt agcatcaagc gtgttatgtg ttcttttttt gactttattt
      961 ttctactata gagacatgct caatttatgt ggtcaaaatt caaatctagg tggggatata
     1021 gagttaaaaa tatattgaca tgtgtaaatt tacatgatat attcatttct gttgttttaa
     1081 gtagcaaagc aataaaatag tattttaaaa aacatataaa atagttgttg acatgttaga
     1141 aatgacatga taagataact gagttgttaa aaaagaacca cttgtgaagc ttgattctat
     1201 cgaaaaaaac ttcaaaaaag ttcttgacaa ataacaaatg ataagttatg ataaatgagt
     1261 tgctgtaaga tttcgaaaaa cgaaataaaa aaaactttta aaaaagagtt gacaattact
     1321 tgtctctctg atatgatata aaagttgcta cggcagccaa aaacgaatcg gggaacactc
     1381 ctgatgaagt agacctttga aaactgaaca aagtaagaca aaccaaatgt gtagggcgtc
     1441 ttgattcaat tcaagacaac aaaca
//
LOCUS       DAYYGS010000204         1463 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000204 DAYYGS010000000
VERSION     DAYYGS010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.212"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(13..1152)
                     /locus_tag="ACHA44_002892"
     CDS             complement(13..1152)
                     /locus_tag="ACHA44_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HFM8906099.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT          384 a          315 c          263 g          501 t
ORIGIN      
        1 attaaaaaaa tcttacgcta agtaggataa aataattcct tgatattttt cgatcatatc
       61 caaataatta tcgatttcaa catactcatc tacttgatga ggcaaagtaa cgacaccagg
      121 cccaaagacg acaaagtcga aggaatggtt agctttcgta aattcggctg catccgttgt
      181 tgctgccgct ccaactaaag gtaatggctg gctgaattgt tgctgaatgc aatgaatcaa
      241 aggagaatct ggatcagctt tgacaggtat tttgttgtaa tcgatcatca attccaaatg
      301 atagtctgtc tcttgattca attcattgac gattgattgc agcaatgcaa tgattttatc
      361 atttgggtat tctggaatcg atcgaatgtt cccttggagc tgcgcatggc ttgggatgct
      421 gttgacttga ttcccgccac tgatcaatgt aacattatgg attgtttttc ctaggacagg
      481 attctcgatt gtttcagcga ggtgattcat ttcagcattt gctttcgtaa tgaattcatt
      541 taggtgattg attgcattat aaccttggtc tggcatagag ctatgcgctt cttttccgtg
      601 cgaggtgact gtatagttga ttgatcccat gtgcgtatac atcaaactgt aattggttgg
      661 ctctccgatg atcaaggcat ctaagtcatc tacatagcct gctttcgtga gctgttcccc
      721 acctagttca cccacttctt ctccgacagt ggctaataat ttgactgttc cgttaaatgg
      781 ttttcctgac tctttcagtt cgatcatggc aatcaccatt gctgccaagc cactcttcat
      841 atctgtagca ccacgtccgt acaaacggtt cccatggatt tctgctgcaa agggtgcata
      901 ggtccaagac gattcatcac cagccgccac gacatccata tgtccagata ggcctaacac
      961 ttttcctgat tggccctttt ggtaacgagc aatcaaatta tctctaccat ctctgtaaga
     1021 aacaatctct cctgttatgt catgctttgc caaaagctta ttcaaatagg ctgctacttc
     1081 accttcgttt ccattcactg atttaattcg gataatctct tgtaaaatcg cgattttttc
     1141 ttcttttttc atctaatcct ctcccttgcc atttcttttt attgtatttg agtgtgctcg
     1201 aattttcaaa taggagcaac caaagtaaaa atgaagaaag agagccactt tatgaaaaat
     1261 atgctgctac ttccctccta ttttatttat acgagaggac ttgcccatag attcctattc
     1321 tactgttcta tccttaaaag ctcttatatg atgctagtta ttgttagctt tcctttttca
     1381 aggatgatct tttcttgtac cattctttgt aaacatcata tacagaagct agttgcaaaa
     1441 aaactctcta cctgctgata ttt
//
LOCUS       DAYYGS010000205         1422 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000205 DAYYGS010000000
VERSION     DAYYGS010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1422
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.214"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..593)
                     /locus_tag="ACHA44_002893"
     CDS             complement(<1..593)
                     /locus_tag="ACHA44_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HFM8906100.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTD"
     gene            complement(752..1018)
                     /locus_tag="ACHA44_002894"
     CDS             complement(752..1018)
                     /locus_tag="ACHA44_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8906101.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1008..1364)
                     /locus_tag="ACHA44_002895"
     CDS             complement(1008..1364)
                     /locus_tag="ACHA44_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFM8906102.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          392 a          274 c          178 g          578 t
ORIGIN      
        1 ttatcagttc cggtaacttt tttgttaatt actgaaggtg tcttgtaaga aatagaatct
       61 aaatcaaagc gttttgattc taaattattt tgtgctttct caattaaata attcactttt
      121 tttgtgagaa accttctgat attgtctttg ctatatgttc cataaaaacc cttatcttct
      181 aaagagtccc tgtacttctt aacttctata tactcttcag ccaaaacttg tggaactggt
      241 tcatctaaga aaaaaacccc tacctcgcct ttgctatttt tataaaagta atcaatttct
      301 gctatagttc ccgatctata attatcaact ggagaaccca acttatggcc aaaaatcgct
      361 aataatatat cactattttc tacaatttgt ttattaataa cactttgccc agagctatta
      421 tttatataag taggaatcgc attcgtttcc catcgaattg gcatcaaaat tacttttgta
      481 ttatctgtat tttctatatt ccatctatat atggtttctt ctacttcatt cctctttgct
      541 gctatatcac ctggtgatgc tataaatact tttattagat ttatggtttt catttattgt
      601 acctttctct ttatgtctct ttaaagtaat ttttgtataa aatttattga cttttacact
      661 ataggacaac ttttatctat ttagtacaaa aaggcccact aaactaattg attagttagg
      721 agcctattac aggtatattt tcaactcgct tttaagcaaa ttctactacc agctttttgc
      781 ctaatgccct agcgatatca tcaagtttat cgaaagatac gttatcccct ctctcaattc
      841 gagcaatagt agattgagtt gtatgtgctt tttgtgcaag ctccgcttga gttaggcctg
      901 cttcctcccg tgcatgataa agagcaaccg cagtttcgag ttttcttcct tcacggtcat
      961 atgcttctgc aaaatctttg tctttttgct tttcttcaat caatttttta atcatcattt
     1021 gtgaaccact cctctctaag ttcttttgca tgtttaagtt cacttattgg tgttttctcc
     1081 gtttttttag taaatccatg ggtaatgaca taccttccat tgaccacatg gaaatagata
     1141 gctctttgga tgtttgatcc taccttactt cttaattcgt acaaattagt gtctaatttt
     1201 ttcacccatt tcaaccgttg agctactagc atcccattct tttctgtttc ttctattgta
     1261 cgtaaaagct tcgcactatc tttttttggt aatgtttcca accattctaa aaattcatca
     1321 tgaccattag gtcttttaaa tgtttcaaaa gatatttttt ccatacccaa ttatatcata
     1381 tattgatttt atattatagt atataacata aaaattcgaa tt
//
LOCUS       DAYYGS010000206         1404 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000206 DAYYGS010000000
VERSION     DAYYGS010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.215"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1235
                     /locus_tag="ACHA44_002896"
     CDS             <1..1235
                     /locus_tag="ACHA44_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFM8906103.1"
                     /translation="ENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRL
                     ARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYP
                     EKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEV
                     SSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQ
                     IGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDA
                     REEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAF
                     SNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKR
                     AWRKMLDDELIPLENELR"
BASE COUNT          513 a          212 c          287 g          392 t
ORIGIN      
        1 aagaaaatag ccgagattca agtgcccaac aagaagaaat gcaaaaaatt attgaggaat
       61 ataatcagta ttatgatact gcctggtcac tagcagacat tgagcgttat aacggagaca
      121 tcaataatcg tttggcacga aaaagagcgg aattcaaaca atttgggaaa caaatcgatt
      181 tagtaatcgt agtggatcgt ctattgacag gttttgacgc ccctacgatt caaaccctat
      241 ttgtggatcg taatctagag tatgcaggat tgatccaagc cttttcacgt acgaaccgta
      301 cgtatcctga aaaaacaaaa ggactaattg ttactttccg aaagccagcg acaatggaga
      361 agaatgtcga ggatgcgaca aaactgtatt cagaagctaa agaagaatcg ggtttgattt
      421 atccaagtta ccaagaatcc aaaaaaagat tcaagcaagc atatggtaag ttaaaagagg
      481 tttcatccat ggaatccatt gatgaacata cgccacttga aatccgtatc gagtacgtca
      541 aagcatttca agaattgaat cgtgcgtatg aatcgttagt gacttatgat gattacaatg
      601 atgacatggt gcaatcttca actttgaaca atcaaattca acttttggaa gaacaaattg
      661 gcgtgtacga gaccgtgaag ggttcattga tggaggaaga accagaaaaa gagggagaag
      721 atttttcaac gatcgaattt tatagtgaaa ataccactaa gttgtatgat attgattcta
      781 cttatattga tcaattacta ggaacttatg ctgcgaatag ttcagatgca cgagaagaaa
      841 ttgaaaaagc tttggctaaa ttgaataaaa cagaaggagt caaagaagtt taccgacaaa
      901 ttttaaatgc atttgatgtg gggactttag atagtaatga agatatcttt gtcattaaac
      961 gtcgctattt tacacaagca agtgataatt tgattcatgc cttttccaat gagtggtttg
     1021 tttctgagaa agaattacat gcttctaatc tgcaatatat gccaggtgaa gatccaattc
     1081 ctaatatgaa agcaattatc aacagtaagg attacgaagg atataaagcc aaacatccag
     1141 aagcgaaacc atttaaatac ccacaagaaa tgaagcgagc atggcgtaaa atgttagatg
     1201 atgagttgat tccacttgaa aatgaattga ggtaaacgag aagagttaag aaaattgtac
     1261 gattcaaaga tagtagtaaa gtggaagtgt agaaaatagg gatagatgtc tttttacttg
     1321 ttcacatttt ataacggagt atttaagaaa gctattgaat aatgattctt ccatcttttt
     1381 cattcattta ccggttctgt tgca
//
LOCUS       DAYYGS010000207         1355 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000207 DAYYGS010000000
VERSION     DAYYGS010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.217"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..1355
                     /locus_tag="ACHA44_002897"
     rRNA            <1..1355
                     /locus_tag="ACHA44_002897"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DAYYGS010000208         1348 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000208 DAYYGS010000000
VERSION     DAYYGS010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.218"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..348)
                     /locus_tag="ACHA44_002898"
     CDS             complement(<1..348)
                     /locus_tag="ACHA44_002898"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8906104.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLF"
     gene            512..718
                     /locus_tag="ACHA44_002899"
     CDS             512..718
                     /locus_tag="ACHA44_002899"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8906105.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            769..1050
                     /locus_tag="ACHA44_002900"
     CDS             769..1050
                     /locus_tag="ACHA44_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8906106.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
BASE COUNT          510 a          167 c          248 g          423 t
ORIGIN      
        1 aaataagtca tatagaccaa gcctatctct gtctttaccg aaatctttga atttgttatg
       61 gaaggtaccg ttgtcaaaaa aaatctcatt gaataaatac ttgatatatt tcttagtttt
      121 ttgagaatta cctgtcagta gatattcagt agaaacacca ccaagtttag caatacttag
      181 tagccgcgct ttgttgggta tgttctttcc tttttcccat ctagaaacat tgcttttgct
      241 tgcgggaggt gtcagatttt cgccaaattc ttcaagagta taccctaaag aaacacgtat
      301 ttcttgtatt cgttttccaa tttcagaatt aatgttttta tcaaacaaaa tggtttcacc
      361 accttcatct attctattct acagtttaca ctacattctc atataatgaa atgaaaaagt
      421 agataaaagt tgaattttac gttgcggtat gaataaatat atgttattct tttagtgacg
      481 aaaagttgaa aagaaggagg agtgaggagt catgaagcct aaagatttta gaaagttttg
      541 tggattatca caatctgaac ttgctgaaaa atatggcatt tcagtacaag cggtaagaaa
      601 caaagaaagc ggtaaaacaa actattctcc agaagaaatg attattttga agtatcttgt
      661 gaaagaaaaa ggaattgaaa atatcacaat tgatcaaatt ttttttaatg aaaagtgacg
      721 aaaagttgaa tttgaataca tgtagtcaaa ttagtggggg caaagaaaat gaaatctgag
      781 attcaagtta ttcttccatc ggatttagat caagaattgc gaagatattt ctatcgagtt
      841 gcaaaagaag cagtagatca agttcgaatt gaaacatctc taagcaaaga agtttttaga
      901 ttgggggaag cttgtaagta tttaagctgt agcagaagta cattaaaaaa atttatcaga
      961 gagaaaggtt taaaagtaag cataatcgga gattgtcaat ttattagaaa agaagaatta
     1021 gcaaggttta tcaaagaata tgaagtataa aaagttgcgt ggcgtgcttt tgatagaaaa
     1081 aaggaagtgt ttgtttgaat aatgatgaag tatagaaaat ataaaaatac tttgaaaaaa
     1141 attaaaagga gtgttcccga cagtttggcg accacaggaa acactcgaca ataaagttct
     1201 aagaaaactt tatttgacta cattttaaca tttattagaa agaagaggaa taaatggaag
     1261 aaatagataa attaattgct cagtatgcat actcaaaaga tgacgtatat gctgaaaaat
     1321 acttgaagta tccgattttt ttagataa
//
LOCUS       DAYYGS010000209         1334 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000209 DAYYGS010000000
VERSION     DAYYGS010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.219"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..1165
                     /locus_tag="ACHA44_002901"
     CDS             86..1165
                     /locus_tag="ACHA44_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HFM8906107.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DAYYGS010000210         1326 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000210 DAYYGS010000000
VERSION     DAYYGS010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.220"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..892
                     /locus_tag="ACHA44_002902"
     CDS             <1..892
                     /locus_tag="ACHA44_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFM8906108.1"
                     /translation="VSVPLILNKREKNNLKKKMLIPFDSEVKGLLNKLTISNIDSTRE
                     KIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITEEKEQQKAIKEQMIE
                     EEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKELYANKIQELEEKLKE
                     LEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEPMDRIKELSSASVPF
                     EFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTV
                     TFTKLAKAEQYRRSLELSKD"
     gene            964..>1326
                     /locus_tag="ACHA44_002903"
     CDS             964..>1326
                     /locus_tag="ACHA44_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFM8906109.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYY"
BASE COUNT          579 a          156 c          212 g          379 t
ORIGIN      
        1 tgtttctgtc cctcttattt taaataaacg agagaaaaat aatttaaaga aaaaaatgct
       61 cattccattt gattcagaag tgaaaggatt actcaataaa ctaactattt ctaatataga
      121 ctcgactagg gaaaaaatca taaaaatatt tgataaaatt aataatttat tcaagaatga
      181 caatgctgaa ttaaggaagg gtcttttaga gtttaaactt caagaactag aacttcatta
      241 ctcttattta gtgaaaatta ccgaagaaaa agaacaacaa aaagcaataa aagaacagat
      301 gatagaagag gaaaaagttc gtagagaaat tgatcgtgaa aagaaacgta ttgataaaga
      361 acaacgtcaa ttcaattctg aaatttccaa attaatcgca tatatgcaaa aagctaatgc
      421 agatgtggaa aaagaattat atgcaaataa aattcaagaa ctggaagaaa aattaaaaga
      481 attagaagtt atacgtgaaa atgtattaca aagagaatta aatactagag ccggttatgt
      541 gtatgtaatt agtaatattg ggtcttttgg tgaagacata tataaaattg gtatgactag
      601 aagattagaa cctatggata gaataaaaga attgagtagt gcatcggttc catttgaatt
      661 tgacgttcat gcgatgattt tttcagagga cgctccgtct ttggaaacta aattgcataa
      721 tcattttaga aaacaagaag ttaataaaat taatcaaaga aaagaatttt tcaaagtttc
      781 actagacgaa atagagaaag ttgttttaga aaattataat ggaacagtaa catttacaaa
      841 attagctaaa gctgaacaat atagacgatc tttagaattg tcaaaagatt aaatatataa
      901 aaagccttcg ggctttttct tttaacaatt aataggaacg tatattcgaa aggaatagaa
      961 ataatgtggt tagaaaaaat aaatgacaaa aaatttaagt acaccgaacg ttatacggat
     1021 cccttaactg aaaaaaagag aaaagtgtcc gttactttat ctagcaattc acgccaagct
     1081 tggaatcaag ctaacttact tttgaatgaa aaaatcgcag aaaaaataaa acgaaatgaa
     1141 gaattaccac taacttttgg agaattaaaa actaaatggg atgaaaaata caagccaact
     1201 gtaaaagaaa gttcctatcg aacaactcag gtttatctat cattaatttc aaaatatata
     1261 aaagatgatg tacttgtaaa aaacgttaat agtaatctta tacaagatat gctagattac
     1321 tactat
//
LOCUS       DAYYGS010000211         1314 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000211 DAYYGS010000000
VERSION     DAYYGS010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.221"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            82..>1314
                     /locus_tag="ACHA44_002904"
     CDS             82..>1314
                     /locus_tag="ACHA44_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HFM8906110.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          444 a          257 c          251 g          362 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg attt
//
LOCUS       DAYYGS010000212         1287 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000212 DAYYGS010000000
VERSION     DAYYGS010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.222"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(62..286)
                     /locus_tag="ACHA44_002905"
                     /pseudo
     CDS             complement(62..286)
                     /locus_tag="ACHA44_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877485.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(840..926)
                     /locus_tag="ACHA44_002906"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="ACHA44_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          454 a          217 c          175 g          441 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattata ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt agtacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacct
//
LOCUS       DAYYGS010000213         1213 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000213 DAYYGS010000000
VERSION     DAYYGS010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.223"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(74..445)
                     /locus_tag="ACHA44_002907"
     CDS             complement(74..445)
                     /locus_tag="ACHA44_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906111.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(447..848)
                     /locus_tag="ACHA44_002908"
     CDS             complement(447..848)
                     /locus_tag="ACHA44_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906112.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            complement(862..>1213)
                     /locus_tag="ACHA44_002909"
     CDS             complement(862..>1213)
                     /locus_tag="ACHA44_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906113.1"
                     /translation="TNVINKVINPALSINAPLIPTALSFSVTAVVSEIEDTEKIKIVE
                     IEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYAVVKFNGTEIGR
                     QIFDIKVNKPVEKN"
BASE COUNT          389 a          215 c          157 g          452 t
ORIGIN      
        1 agtatacttt cgttgatcca gaaagataca aagattaaat aaaaaaataa caaacaaact
       61 cgcttgaaat tcattaggtg agtttgttta ttattttttc tgcttctcga ttcatcagcg
      121 ccatatctgt tttcgtaatg gtttcattag gaatatagtc agcacatttt cttttctcta
      181 acattctata cccactatta tgtattccta aggtattcaa ttccttaact gttgaaaaca
      241 aattccaaac ggtttggtgg cttccttttc cgctacttcc attataaata atatcataat
      301 tttctttcaa atactcatca gattttttaa aagcactgta gtatgctctt gaaacagcag
      361 ttcttctata agattcttta gaattatatt tatcatctga ttcaatttgt ttggaaatag
      421 ataaatattc attaaagtca aacattttat tcccccaaag ttgctactac agatttcccc
      481 atatatttat agtcagataa tacattatcg agagtatcca aaacatcgta atctgaggaa
      541 aacaattcag atgttgtttt gataacaaag aaatacaagt ttaaatcttc atcatatttt
      601 atattgattt cagcttttct ttttaaaacc gtatttatca catcaaaaat tttgccatca
      661 ttacttgaaa ttgtgttaat ctcatttttt gtagtaatta caagttgatt atcattttta
      721 gttttcggag tagaacttgc atccacaaca actggagatg ctagaattac cgatgcgcaa
      781 gcaaaaatcg cagcaaaatt tattaagcta ccactccgat gtgtatttga tggtactata
      841 tcaagcatcc gtatcgtctc cttaattttt ttccactggt ttgttgacct tgatatcaaa
      901 gatttgcctt ccaatctcag ttccattaaa tttaacaaca gcataatgct ctcctgcttc
      961 ttcaaccaag acatttctgg cgtttatatt aaagtttata tcgttaactt gaggaggcaa
     1021 gcttaccgat acttcacccg tagaaaaaat ttgtttttca ttcttgttta aaacttcaat
     1081 ttctactatt tttatctttt ctgtatcctc tatctcagac accacagctg ttacagaaaa
     1141 tgataaagca gtaggaatta aaggcgcatt aatagaaaga gctggattaa ttactttatt
     1201 aataacatta gtg
//
LOCUS       DAYYGS010000214         1192 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000214 DAYYGS010000000
VERSION     DAYYGS010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.224"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            77..949
                     /gene="scrK"
                     /locus_tag="ACHA44_002910"
     CDS             77..949
                     /gene="scrK"
                     /locus_tag="ACHA44_002910"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HFM8906114.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          355 a          221 c          282 g          334 t
ORIGIN      
        1 aataaaaaaa taatttaaat atgatatata gtcatttatt tagtgatttc attctaaaat
       61 aaagggagaa atgtctatgc tatacggtgc aattgaagca ggcggcacaa aatttgtgtg
      121 tgccattggc gatgaagaaa tgacgatcaa ggaaagagtg agctttccga caacgacacc
      181 tgaggaaaca atgcctttag tcattgactt cttcaaacaa taccaagcag atttagcagg
      241 gattgggatt ggctcatttg ggcctatcga tatccatcgc gattccgcca cttatgggta
      301 cattacctcg accccgaaat tggcatggca aaattttgat ttcatcggta ccatgaaaaa
      361 agaatttccg atcccgattt cttggacaac agatgtcaat gcggctgcct atggtgagta
      421 tgtcttcggc agtggcaaag gattgagcag tgtcgtttac tatacgatcg gtacgggtgt
      481 tggcggcggt gccttacaag aagggcgctt tatcgaaggg tttagtcatc cagaaatggg
      541 gcatatgctc gtcgtccctc atccaaaaga tgactttgct ggcagctgtc catttcatgg
      601 caactgttta gaagggatgg ccgctggacc cgccatcgaa aaccgcttag ggaaaaaagg
      661 acaagaggtt gcagaagatg atccttattg ggaaatcgaa gcctcctata ttgctcaatg
      721 tgcctacaat acaaccttga tgttttctcc agatgtgatc atttttggcg gtggtgtgat
      781 gaaacaacgc catatgctgc aaaacgtcca cgacgcattt gcaaaattgg tcaatgggta
      841 tgtcgaaaca cctgagttaa gcaaatatat cgtgacccca gcattagaag acaacgctgg
      901 aacgctcggt tgtttggcat tggctcgaga agcggtctta cattcataaa cgaaaaaagg
      961 ctcttcgaca tttagtgttg gtagagaaag atacaactga aaatttagtc ataaccagtt
     1021 aggcagagtg cttaactggt ttttgtttta cttggaagat caagccttga atgagataaa
     1081 ttcttgctcg aaagttatgg aaatttcgat agccgtaggc cacttgtatt gtgcaatagt
     1141 tttttacaaa aaaaaggggg tgcggatatt tttttggacc tttttaacaa at
//
LOCUS       DAYYGS010000215         1151 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000215 DAYYGS010000000
VERSION     DAYYGS010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.226"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            172..513
                     /locus_tag="ACHA44_002911"
     CDS             172..513
                     /locus_tag="ACHA44_002911"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906115.1"
                     /translation="MSIDDCLEEQIRLFLKFLKVKVQNNYFTCYSYAHPVLNSLPFNS
                     LVQGLITNIVLLVCTPNEIFVKKIGSGISSVRLKEKEYTQKIIHIPKPEIKRFEIKDY
                     SALGLFSIGYW"
     gene            881..1072
                     /locus_tag="ACHA44_002912"
                     /pseudo
     CDS             881..1072
                     /locus_tag="ACHA44_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          424 a          126 c          189 g          412 t
ORIGIN      
        1 ttcggtaaaa ctactttttc tagttttaat tttcagtttc tattaaaaaa taatttttat
       61 ggtttattga aataaggttg cgaaggaaga tgtagagtta tttgcactgt tgtgttggta
      121 tatctcttaa tgctgtgtct ttttggggag attaattagt ggagtgatta tatgagtata
      181 gatgattgtt tagaagaaca aataagatta tttttaaagt ttttaaaagt taaagttcaa
      241 aataactact tcacatgtta tagttatgca caccctgttt taaatagtct tccttttaat
      301 tctttagtgc aaggattgat tacaaatatt gttttattag tctgtacacc aaatgaaata
      361 tttgtaaaaa agataggcag tggtatttct tcagtaagat taaaagagaa agaatatact
      421 caaaagatta ttcatattcc taagcctgaa attaagcgtt ttgagataaa agactattct
      481 gctttagggc tgttctcaat tggttactgg tgataaaaaa taagaatact aaaactatac
      541 gtagaatata agtaatcggc atcaatccta attatgtgat tgaatgccga tttttgttac
      601 gcgctatttt taattatctg taagaagaat aattttattt tactcatatt cgttttcact
      661 ttgaagaagt aaattgcaat ttcttataag ttaagcagct atgcaaaagg ctatttattc
      721 tattgtgaaa tataagatgt agttgaaaca ggatatatag aatcaaaata cgaatcttat
      781 tttgattttt ctagtttttt gaaaaaagca ccctttgatc gttgtattag tggaaaagag
      841 aaaaaggagc atacttatca tgtcacaaaa ctacaactaa atatctaaac aacaccaatt
      901 tgatgcctat tgaaaaaaag tattgcgaaa tgaagccaag tctattagaa aatgaaataa
      961 tagaataaaa gaaaatggga aaccgctgaa tgatgtaaat gaaggtaagt atccacgaac
     1021 taattttgat gaacaagata tctatttttt atttgtattg gagatactag tttaatatca
     1081 aaagttatca gcagtcataa ttagttatcc aatgtaaaaa gtacaaacaa tagttgttat
     1141 gcagaatagt t
//
LOCUS       DAYYGS010000216         1094 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000216 DAYYGS010000000
VERSION     DAYYGS010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.227"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            48..590
                     /locus_tag="ACHA44_002913"
     CDS             48..590
                     /locus_tag="ACHA44_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906116.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            746..>1094
                     /locus_tag="ACHA44_002914"
     CDS             746..>1094
                     /locus_tag="ACHA44_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFM8906117.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          467 a          114 c          214 g          299 t
ORIGIN      
        1 aaaataaaaa attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag
       61 tagctataaa atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac
      121 caagaggagc atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa
      181 aaagtaatga aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca
      241 tgtcaattaa tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg
      301 caaagaaaga atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta
      361 gaaattatat agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag
      421 aactaaagta tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta
      481 tagaaaagat gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta
      541 ttggtatatt tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac
      601 tcatactaaa ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag
      661 cggagctgaa agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa
      721 aaaagaacgt cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga
      781 agcatttaac aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg
      841 caaggatttt acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc
      901 aacttggtgt acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat
      961 gaaaagcaag ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga
     1021 aaacaaggaa gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc
     1081 tttcttaatg ccag
//
LOCUS       DAYYGS010000217         1037 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000217 DAYYGS010000000
VERSION     DAYYGS010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.229"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(20..91)
                     /locus_tag="ACHA44_002915"
     tRNA            complement(20..91)
                     /locus_tag="ACHA44_002915"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="ACHA44_002916"
     tRNA            complement(113..200)
                     /locus_tag="ACHA44_002916"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="ACHA44_002917"
     tRNA            complement(225..298)
                     /locus_tag="ACHA44_002917"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="ACHA44_002918"
     tRNA            complement(312..382)
                     /locus_tag="ACHA44_002918"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="ACHA44_002919"
     tRNA            complement(384..459)
                     /locus_tag="ACHA44_002919"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="ACHA44_002920"
     tRNA            complement(478..551)
                     /locus_tag="ACHA44_002920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
     gene            complement(564..653)
                     /locus_tag="ACHA44_002921"
     tRNA            complement(564..653)
                     /locus_tag="ACHA44_002921"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(615..617),aa:Ser,seq:tga)
     gene            complement(677..750)
                     /locus_tag="ACHA44_002922"
     tRNA            complement(677..750)
                     /locus_tag="ACHA44_002922"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Ile,seq:cat)
     gene            complement(764..837)
                     /locus_tag="ACHA44_002923"
     tRNA            complement(764..837)
                     /locus_tag="ACHA44_002923"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Met,seq:cat)
     gene            complement(865..937)
                     /locus_tag="ACHA44_002924"
     tRNA            complement(865..937)
                     /locus_tag="ACHA44_002924"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Ala,seq:tgc)
     gene            complement(957..1030)
                     /locus_tag="ACHA44_002925"
     tRNA            complement(957..1030)
                     /locus_tag="ACHA44_002925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(994..996),aa:Pro,seq:tgg)
BASE COUNT          281 a          285 c          246 g          225 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc tccatatgaa
      661 acttacaacg ccacaatgga ccttgtagga ctcgaaccta cgaccggacg gttatgagcc
      721 gtctgctcta accaactgag ctaaaggtcc aggctcttaa aaatcgcggc ggaggggatc
      781 gaacccccga cctcccgggt atgaaccgga cgctctagcc agctgagcta caccgcgaaa
      841 aagatattca ttatttaaat gaaatggagc ctagcgggat cgaaccgctg acctcctgcg
      901 tgcaaagcag gcgctctccc agctgagcta aggccccgtg tttaaattta taattatcgg
      961 gaagacagga ttcgaacctg cgaccccttg gtcccaaacc aagtgctcta ccaagctgag
     1021 ctacttcccg tttaatt
//
LOCUS       DAYYGS010000218         1035 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000218 DAYYGS010000000
VERSION     DAYYGS010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.230"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>1035)
                     /locus_tag="ACHA44_002926"
     rRNA            complement(<1..>1035)
                     /locus_tag="ACHA44_002926"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAYYGS010000219         1031 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000219 DAYYGS010000000
VERSION     DAYYGS010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.231"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..592
                     /locus_tag="ACHA44_002927"
     CDS             <1..592
                     /locus_tag="ACHA44_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906118.1"
                     /translation="PTLITKKELLKKLDISTGVLANLIRNGMPKEGEMFNLDKIITWR
                     ENWSKNILGELEVGRVYTNKEISEKFKCSKQGGMRRSHQTNTLVLFSDQTGSNVYKDK
                     WLNGILQYTGMGLKGDQVLDKNQNKVLANSKSNFVKIHLFETFKPKEHTYLGEVYLAG
                     QIYTVNEKDSSGNSRKVYKFPLALINQEQLIEDKDI"
     gene            573..1019
                     /locus_tag="ACHA44_002928"
     CDS             573..1019
                     /locus_tag="ACHA44_002928"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFM8906119.1"
                     /translation="MKIKIYNQEENQTRHIRNLSNAKLEEEARKVSNYNKKMAAKYVN
                     EEEFCFSKTKIYKRNPFVSEYVKRLAKGICQLCQEKGPFIKDGVPYLHCHHIEYLSQG
                     GKDVIENCIALCPNCHARIHELELPDDKEKLLKIVEKRENSFSKIP"
BASE COUNT          423 a          118 c          185 g          305 t
ORIGIN      
        1 tccaacattg attactaaga aagaattact aaaaaaatta gatatttcta ctggtgtatt
       61 agctaattta attagaaatg gcatgccaaa agagggagaa atgtttaatc ttgataagat
      121 tattacatgg cgagaaaatt ggtctaaaaa catattaggt gagttagaag ttggtagagt
      181 atatactaac aaagaaattt cagagaagtt taaatgttcc aaacagggtg gaatgagaag
      241 atctcatcaa actaatactc ttgtactgtt tagtgatcaa acaggaagta atgtttataa
      301 agataagtgg ttaaatggta ttttacaata taccggtatg gggctaaaag gggatcaagt
      361 attggataag aatcaaaata aagttttagc caattcaaaa agtaattttg taaagattca
      421 tttatttgaa acatttaaac caaaagaaca cacatatctg ggagaagtat atttagcagg
      481 acaaatatat actgttaatg aaaaagatag ttcgggcaac agcagaaaag tatataaatt
      541 tccattagct ttaataaatc aagagcaatt gattgaagat aaagatatat aaccaggaag
      601 aaaatcaaac tagacatatt cgtaatttat ctaatgctaa attagaggaa gaagcaagaa
      661 aagttagtaa ttataataaa aaaatggctg caaaatatgt taacgaagag gagttttgct
      721 tctcaaaaac aaaaatatat aaacgtaatc ccttcgtatc tgaatatgtt aaacgacttg
      781 caaaaggtat ttgtcaactt tgccaagaaa aagggccatt tataaaagac ggtgtacctt
      841 atttacattg tcatcatata gaatatttgt cgcaaggtgg aaaagatgtg attgaaaact
      901 gtatcgcgtt atgtcccaat tgtcatgcta gaattcatga acttgagtta cctgacgata
      961 aagaaaaatt gctgaagatc gttgaaaaaa gagaaaatag cttttctaaa ataccgtaat
     1021 ttatttttat a
//
LOCUS       DAYYGS010000220         1009 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000220 DAYYGS010000000
VERSION     DAYYGS010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.232"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>1009
                     /locus_tag="ACHA44_002929"
     CDS             <1..>1009
                     /locus_tag="ACHA44_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HFM8906120.1"
                     /translation="INNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSL
                     ATLSLIVFLLIGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFK
                     YLILSFISTFLNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHF
                     FDKKQWRYALKISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSV
                     IWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKD
                     YYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPR
                     LG"
BASE COUNT          307 a          126 c          170 g          406 t
ORIGIN      
        1 gattaataat ttcacttcat ttgtatcaat tttttcgatt cttgttgggc taagcctaaa
       61 ttcggcaatt aataatgcaa gttttgacta taaagatgac ataaatgaat ttatgtcatc
      121 ggtactttct ctagctacac tttcgttaat tgtgttctta ttaataggga acatatattt
      181 tttgttttca aatgcttttt ttgatatgaa ccaacttgta tttaatttga tgttattaca
      241 aagttttgct agttttgtaa ttagttatat ccagtcttat tttacgatta actttaacca
      301 ttttaaatat ttgattttat cttttatatc tacttttttg aatattgcaa tctctgtagc
      361 tttgatgcag acattatatt ttgatgataa atatgttggc agagtagtcg gcggaacact
      421 gtcatttgtg ttgataggtt tagtaattta tttaagaata atgataaagg gtgctcattt
      481 ttttgataaa aaacagtgga gatacgcttt aaaaatctct ttgccgctaa tcccacattc
      541 cttagcaaat atagttttag cacaattcga tagaattatg attaattctt atttaggtcc
      601 tgctgcgggg ggaatatatt cctacattag taacataggt attattctaa gcgtaatatg
      661 gatgtctact aataacgcct gggttccatg gttctatggt gagatgagta aaaataatga
      721 aaaaaaagtg aagaaagtta gtaactatta tttagtagtc tttactttga ttacgatgat
      781 tgtaatggta gtttcgattg atttagcaaa atttatggct ccgaaagact attattctgg
      841 gttatcttta gtggttccga taaccttagg ctatttcttt cagtttttat atagtttacc
      901 agttaatgct gaattttatg agaaaaaaac atcgtttatt gcgattggta cagtagcaag
      961 tgctatagtt aatatactat taaacatgat tttcatacct aggttagga
//
LOCUS       DAYYGS010000221          997 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000221 DAYYGS010000000
VERSION     DAYYGS010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.233"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            365..997
                     /locus_tag="ACHA44_002930"
                     /pseudo
     CDS             365..997
                     /locus_tag="ACHA44_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          342 a          181 c          183 g          291 t
ORIGIN      
        1 aaagttagta gagaaaatgt agttatatat ttaaattttt accagaaaga aatgaacacc
       61 ttcaattatc taaatctcga aaatggttag atgtaatcat caggaaaaac aatcttaaac
      121 gaattactat tcatggtctt agacacacac atgctagctt gcttttagca agctagtgca
      181 aatattaaag atgtgcaaga gcgtttaggc cactcttcta ttcaaatcat tatggatcta
      241 tatattcata ttacagacaa aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg
      301 ctctataatt tatgggactg atgaatcaaa attgattctt cagtcccatt gttattttag
      361 gtatatgatt cgtacattga aagagttgaa aaactatctt ccgaagccgt caaaaaggac
      421 cctgccaaaa gtattgatgg tggatgaatt tcgttcgcac gcttcaattg tggataaaat
      481 gagctttatt tgtgcggatg gcgagactgg aaaactcatt gatattttgc ctacacgaaa
      541 attgtctcga ttaacacact attttagtcg ctgtgcaaat cctgaagcgg tggaatattt
      601 agtgacagat atgaacgccg cctacttcca actgacaaaa cgtgtcttac caaatgcgaa
      661 agtcgtcatt gattgttttc atatcatcaa acatttgaat caggcgttca atgattttcg
      721 tgtacaagaa atgaaaagat tacaccagtt agggcaacga cgggaagccg agaaagtgaa
      781 aaagaactgg cgctttcttc taaaaaaccg tcaaaaaatc aatcactatg attacaaaac
      841 atggcgcagt tttcgtgcac ctaaataccc gctactcact gaagcaatga tgattgatcg
      901 attgctggca ttttcaacgc ctttaaagga cgcctatact tttttccatg agctgaccga
      961 agcctttaga gacaaggaat cagacttatt ttttacc
//
LOCUS       DAYYGS010000222          986 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000222 DAYYGS010000000
VERSION     DAYYGS010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.234"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>986
                     /locus_tag="ACHA44_002931"
     CDS             <1..>986
                     /locus_tag="ACHA44_002931"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFM8906121.1"
                     /translation="LEPGDFIMSGAGTIGRISRVPKQIKPGVFNQALIRLRINKEITD
                     SEYFIQFIRADFMQRKLTGANPGSAITNLVPMSEVKKWIVQFPILEEQKKIGNFFKQL
                     DDTIALQQRKLDLLKETKKGFLQKMFPKNGAKVPEIRFPGFTEDWEQRKFKEFSKKTG
                     KKNTKDLDFPAYSVSNKAGLISQTEQFDGSRLDDLEKTNYKFVEPNEFAYNPARVNVG
                     SIAFNNLGMTVIVSSLYVVVKMSEDLDNEFILQFIKSPTFIKEVKRNTEGSVREYLFY
                     ENFANIKFPFTRNKEEQQKIGAFFKQLDDTIALHQRKLDLLKETKKGFLQKM"
BASE COUNT          375 a          159 c          189 g          263 t
ORIGIN      
        1 ttagaaccag gcgattttat catgagtggc gctggaacaa ttgggagaat ttcaagagta
       61 ccaaagcaaa taaaaccggg tgtattcaat caggctttga ttagacttag aataaataaa
      121 gaaatcacag attcagaata ttttattcaa tttattcgtg cagattttat gcaaagaaaa
      181 ctcacaggag ctaatccagg ttcagcaatt acaaatctcg taccaatgtc agaagtgaaa
      241 aaatggatag tacaatttcc aatattagaa gaacaaaaga aaattgggaa cttcttcaaa
      301 caactggacg acactatcgc tcttcaacaa cgtaagctag atttattaaa ggaaacgaaa
      361 aaaggattct tacaaaaaat gttccctaaa aacggagcaa aagtgccaga aattcggttt
      421 cctggattta cggaagattg ggaacagcgt aagttcaaag agtttagtaa gaagactgga
      481 aaaaagaaca cgaaagattt agacttccct gcctattcgg taagtaataa agcaggttta
      541 atttctcaaa cagaacagtt tgatggtagt aggttggatg atttagaaaa aacaaattat
      601 aaattcgttg aaccaaatga attcgcctat aatccagcaa gagtaaacgt aggatcaatt
      661 gcatttaaca atcttggtat gacggtcatt gttagctctc tttatgtagt tgtgaaaatg
      721 agcgaggact tagataatga gttcatactt cagtttatca agtcaccaac atttatcaaa
      781 gaggtcaagc gcaacactga aggaagcgta cgagaatact tattctatga gaatttcgct
      841 aatatcaagt ttccttttac taggaataaa gaggaacaac aaaaaatcgg cgctttcttc
      901 aaacaactcg acgacacgat cgctcttcat caacgtaagt tagatttgct gaaggaaacg
      961 aaaaaaggct tcttacaaaa gatgtt
//
LOCUS       DAYYGS010000223          943 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000223 DAYYGS010000000
VERSION     DAYYGS010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.235"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>943
                     /locus_tag="ACHA44_002932"
     rRNA            <1..>943
                     /locus_tag="ACHA44_002932"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAYYGS010000224          941 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000224 DAYYGS010000000
VERSION     DAYYGS010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.236"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..741
                     /locus_tag="ACHA44_002933"
     CDS             <1..741
                     /locus_tag="ACHA44_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HFM8906122.1"
                     /translation="NLSKTIEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNL
                     ENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQE
                     NELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIA
                     NYFSVSETKLRESFKNEMHITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSH
                     FSRVFKKYAGVSPKQYQLGLVDNFNNIS"
     gene            758..>941
                     /locus_tag="ACHA44_002934"
     CDS             758..>941
                     /locus_tag="ACHA44_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906123.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCE"
BASE COUNT          351 a          105 c          167 g          318 t
ORIGIN      
        1 aatctaagta aaactatcga gcaaatcaaa atagataatc tgttatcaca aaattacgat
       61 ccagaaatat atctgtttct ttatgaaaat aaaatactgg aatatgttaa aaatggagat
      121 attcgaaatt tagaaaacat ggtttttaat ctgagtaatg gaataattcc aagtgttagt
      181 ggagatacga ttcggtctga aaaaaattat tctatcattg tttttgaaaa gttagcacaa
      241 acgtctataa cattaggaat ggatataata gaagcgtatc aaagtagaga tgctttgata
      301 caagaaaacg aattagctgt atcgttacct gaggtcttaa aagtgaggga ttctgggatt
      361 gtttattata ctaaggaaat cggaaaaact aagatagagc atctttctcc cttaatttct
      421 tctgtcgttc aattcatcgg cttaaatatc tataaaagaa ttactgtaaa ggaaatagct
      481 aattattttt ctgtaagtga aactaaatta agagaaagct ttaagaatga aatgcatatt
      541 acgatttata attatatctc taagcgaaaa atttctacgg ctaaaataat gttgaaatca
      601 aaccatacta tcagtgaagt ttctttaggt ttaggatttt cagattcttc acacttttcg
      661 agagtattca aaaagtacgc aggtgtatct cctaagcaat atcaattagg tttagtggac
      721 aatttcaaca acattagctg agaggtttta ttttattatg agactatatc atttacgtag
      781 tttggaggag ttgaggaaat tggattttaa caaaaaattt ttttgggaaa tttctgattt
      841 gagttgtttc gtagaatctg agcgtctaag taatggtaat aatagtgtgt taaatgaata
      901 tgacaaagta gtaattaata aagtaaaaag tttatgtgaa g
//
LOCUS       DAYYGS010000225          915 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000225 DAYYGS010000000
VERSION     DAYYGS010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.237"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            86..>915
                     /locus_tag="ACHA44_002935"
     CDS             86..>915
                     /locus_tag="ACHA44_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HFM8906124.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          317 a          136 c          158 g          304 t
ORIGIN      
        1 aatagatatt tagggtagaa aaaaagaaag tccattctgt aaaattaaag ttaccacaac
       61 cgaaatttta taggaggact ttctcatgcc caattttact acagaaatta tggaaacact
      121 aatcaataaa ggtgatttag atgaattatt tcgatgtcat ttagaattag ctgttaattc
      181 attattgcag gctgaattaa cagcttttct tgattatgaa aaatatgatc gtgctgggtt
      241 caattcaggt aattcccgta atgggaatta ttcacgttca ttcagaacag agtatggaga
      301 attaaatttg gtaattccta gagatagaaa tggtaagttt tctcaacaaa ctttacctgc
      361 ttataaaaga accaatgact ctttagaaac cactattatt cagctatttc aaaaaggaat
      421 tactatgtct gaaatctctg aattgattga aaaaatgtac gggcatcact atacgccaca
      481 aaccatttca aatatgacta aaatagtttc tgaagatatt attgccttta aagaaaggtc
      541 cttagaatcc agatactctg tcatttttat ggatgctact cacattcctt taaaaagaca
      601 aactgtttca aaagaagcag tttatattgt aattggcatt cgattagatg gaaccaaaga
      661 ggttcttggg tttagtcttg ctccaactga atctgcttat gtttggaaag agattcttca
      721 agacctaaaa gaccgtggtt taaaagaggt tttattagtc gtaactgatg gtttaagtgg
      781 cattaacgat agtattcata gtgtttatcc aaatgctcaa tttcaacaat gttgtgtcca
      841 tatatctaga aatatcgctc ataaggttcg tgttagtgat cgacaagaaa tctgtagcga
      901 tttcaaatta gttta
//
LOCUS       DAYYGS010000226          889 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000226 DAYYGS010000000
VERSION     DAYYGS010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..889
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.238"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            6..>889
                     /gene="bsh"
                     /locus_tag="ACHA44_002936"
     CDS             6..>889
                     /gene="bsh"
                     /locus_tag="ACHA44_002936"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HFM8906125.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQIT"
BASE COUNT          304 a          122 c          160 g          303 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctattactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctggactaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattac
//
LOCUS       DAYYGS010000227          872 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000227 DAYYGS010000000
VERSION     DAYYGS010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.239"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>872
                     /locus_tag="ACHA44_002937"
     CDS             <1..>872
                     /locus_tag="ACHA44_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HFM8906126.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYV"
BASE COUNT          295 a          149 c          178 g          250 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gt
//
LOCUS       DAYYGS010000228          866 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000228 DAYYGS010000000
VERSION     DAYYGS010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.240"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(98..>866)
                     /locus_tag="ACHA44_002938"
     CDS             complement(98..>866)
                     /locus_tag="ACHA44_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFM8906127.1"
                     /translation="ITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWM
                     VSILLVLLVYFSFGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSD
                     NSEISAGRFTIWKAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAH
                     GNMHNGFLQVLVSSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIF
                     IDEMVEAHVLFNKRDVISIVFWYYLGSISYLYLKKEN"
BASE COUNT          332 a          150 c          105 g          279 t
ORIGIN      
        1 aaataaatct ccttttgatt ctcggtaaaa attttgataa acatttaata tattaatcaa
       61 atgtaaagga gtccaagcaa tgaaagctgt cttcatatta attctccttt tttaaataca
      121 agtaagaaat gcttcccaaa taataccaga aaacaattga aatgacatcg cgcttattga
      181 aaagtacatg agcctccacc atttcatcta taaaaataga tagtataaaa attaaaacaa
      241 taccaagaaa cgcataattc actttacttc taaaaaatgt tttgagtaaa ctaaagatat
      301 ttaacacata gaaagcatag atacatataa aagcaattat accacttgat acaagtactt
      361 gaagaaatcc attatgcatg tttccatgtg ctctttttag ttcattcaca tctgtagtat
      421 caagatctac ttttttcaaa aattttgcat cgctattccc atagaaatcg gtctctccat
      481 aaccaaataa caaagttggt cttataactt taagcgcagc tttccaaatc gtaaaacgac
      541 cagctgaaat ttcggagtta tcactagaat gctgtaatat tacttctttt tctggaagtg
      601 gagttttatc tgattcattt gactctataa cttctacttt tctatcaatc gatacaggaa
      661 ttgttgctaa aaaatcctta gcataattag taccaaaaga aaaatatacc aatagaacta
      721 ataatatgct taccatccat ttgattttta caggtagtac ttctctaatg ctctttctaa
      781 attttgaatt ggcaaacaac aaaagaaata atactaaaaa agaacaaatt actacctggg
      841 cacctctaga cgaagaaagt gtaata
//
LOCUS       DAYYGS010000229          841 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000229 DAYYGS010000000
VERSION     DAYYGS010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.241"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACHA44_002939"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="ACHA44_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFM8906128.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(591..785)
                     /locus_tag="ACHA44_002940"
     CDS             complement(591..785)
                     /locus_tag="ACHA44_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906129.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          244 a          168 c          146 g          283 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc atttaattta
      781 tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag
      841 t
//
LOCUS       DAYYGS010000230          832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000230 DAYYGS010000000
VERSION     DAYYGS010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.242"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(40..339)
                     /locus_tag="ACHA44_002941"
     CDS             complement(40..339)
                     /locus_tag="ACHA44_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5960 family protein"
                     /protein_id="HFM8906130.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKDVYFLFDIVTDVLDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(345..575)
                     /locus_tag="ACHA44_002942"
     CDS             complement(345..575)
                     /locus_tag="ACHA44_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906131.1"
                     /translation="MMEEFIRKNISDEYADFYEQSNEKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
BASE COUNT          289 a          145 c          114 g          284 t
ORIGIN      
        1 ataaaatccg ttccgggcta cctttttagg tagcctactt taatctttgt atctttctgg
       61 atcaacgaaa gtatacttta tatagtcata acgccgatga tcgcttcgag cgtctagcac
      121 atcagtcacg atatcaaaca aaaaatatac gtctttcttc attctagttt tcgcagcagg
      181 gattttaaag tagttcttat tagaatagta gagattaatt aataagctat cttcgattgc
      241 taaaaagaaa acttctgaat cccacacctt ataaaaatct ttgacaaatc tattcgaagg
      301 atcaaattta aaccataatt gcgtctcatt aaaaagcata accattactc cgatctattt
      361 ttaaacttag tctctacctc taatatatat cgagttttta ttttgccttc agagaatact
      421 gtttcttttt ttgcagttac aggttgttta ttttcggaaa aagctaatat agctaaaatt
      481 gaaacatcca tctggaattt gtctttttcg ttgctttgct cataaaaatc tgcatattca
      541 tcactaatat tttttctaat aaattcttcc atcataaaga tcacctcgaa aagagtatac
      601 gaacaaacgt tctttttgta aaggtggaat ttattatact aaataaatag gtgaacaagt
      661 acttgtgcca aattatgtgc caaaaaaaat cgaatttaat aaaacacaaa caaaaagaaa
      721 tcctattatg ataacatttc ttataataac aaacacaaca gaagacgtgt aatagttagt
      781 caggaacgta caaataaccc ctgtatcctt tgcggtacag ggggttattt tg
//
LOCUS       DAYYGS010000231          811 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000231 DAYYGS010000000
VERSION     DAYYGS010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.243"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            3..>811
                     /locus_tag="ACHA44_002943"
     CDS             3..>811
                     /locus_tag="ACHA44_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HFM8906132.1"
                     /translation="MMEILINKGDLDDLFRRHLELAINALLQAELTVFLDYEKYDRAG
                     FNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLETTIIQLVQ
                     KGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVIFMDATHI
                     PLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRGLEEVLLV
                     VTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV"
BASE COUNT          278 a          117 c          149 g          267 t
ORIGIN      
        1 aaattatgga aatactaatc aataaaggtg atttggatga tttgtttcgt cgtcacctag
       61 aattggctat taacgcttta ttacaagctg aattaacggt atttcttgac tatgaaaagt
      121 atgatagagc tggatttaat tcaggtaatt cccgtaatgg gaattattcg cgctctttta
      181 aaacagagta cggtgaattg aatttagtaa tacctagaga taggaatgga gacttttctc
      241 aacaaacttt acctgcttat aaaagaacca atgactcttt agaaaccact attattcagc
      301 tagttcaaaa aggaattact atgtccgaaa tctctgatct aatcgaaaaa atgtatggtc
      361 attattacaa accacaaact atttcaaata tgagtaaaat tgtgtctgaa gatgtttcgg
      421 catttaaaga aagaacttta gaagctaaat actcagtcat ttttatggat gctactcata
      481 ttcccttaaa aagacaaacc gtatcaaaag aagctgtcta tatagtaatt ggtatcaggt
      541 tggatggaac taaagaggtt ctaggattta ctatagctcc aactgaatct gcttatgttt
      601 ggaaagagat tcttcaagat ctaaaagacc gtggtttaga agaggttttg ttagtcgtaa
      661 cagatggttt aagtggtatt gacgatagta ttcatagtgt ttatccaaat gctcaatttc
      721 aacaatgttg tgtccatata tctagaaata tcgctcataa ggttcgtgtt agtgatcgac
      781 aagaaatctg tagcgatttc aaattagttt a
//
LOCUS       DAYYGS010000232          795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000232 DAYYGS010000000
VERSION     DAYYGS010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.245"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..274
                     /locus_tag="ACHA44_002944"
     CDS             <1..274
                     /locus_tag="ACHA44_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HFM8906133.1"
                     /translation="LLNIWIFDRSRNASIGFRTKRSLSSKKNWVYSQTIFYGGIVLIS
                     LLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(365..730)
                     /locus_tag="ACHA44_002945"
     CDS             complement(365..730)
                     /locus_tag="ACHA44_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906134.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          298 a          104 c          142 g          251 t
ORIGIN      
        1 acttctgaat atatggattt ttgataggag tagaaatgca agtataggtt ttagaactaa
       61 acgaagtctg tcttctaaaa aaaattgggt atactcacaa actatttttt atggaggaat
      121 tgttttaatt agtttacttt cttcaacact ttattctttg aatattatag atgtttccac
      181 ctcaaattca atatctatta ttggaatcat tattgctgca attattacac aactgttttt
      241 agtgtttgga gagaagaaaa gaagtaaaaa atagaaaaac tagtgatagt tttggtgata
      301 taagaaaaac atatatgtat aaaaagaaga aagtagcttc tctttgtaca tatatggttt
      361 gtttttatgg atgccgagct tttttataag ataaggctac cgcccataac gacccaagga
      421 tttgtggaac taagtaaata cctgcaagaa gcaagatcag ctgggtcggt tttaaaggat
      481 gatagaagta acgccattca gtaaaattgg cgcaaaataa ataggaagtg acagtagata
      541 taatattggc aatgaataaa gatataatac gcctgttcct ttgaaaataa tatccagcaa
      601 aaagagataa aaatatcaat gtgatgaacc cgcttacgga atgataacga aaatcaagca
      661 gtaaggcgaa atgcacataa ggaacaaaag ataaaagcaa cggaaggttt ttccagcaag
      721 tttttatcat ctttcatcac cacctaacaa gtgttattat tttgttagtt ttattgtaaa
      781 ttgaatcctg caata
//
LOCUS       DAYYGS010000233          787 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000233 DAYYGS010000000
VERSION     DAYYGS010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.246"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            264..587
                     /gene="relB"
                     /locus_tag="ACHA44_002946"
     CDS             264..587
                     /gene="relB"
                     /locus_tag="ACHA44_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HFM8906135.1"
                     /translation="MRFFKRGNYRAMITVRVSDAKKEWLSYMAEFYGITLSELLKNYS
                     MEQLEDEYNRQTAEIAHKHWIRSGKTTVFMEESLMNLVGWNDLPFKIHARNSEAALIG
                     KYVYW"
BASE COUNT          274 a          113 c          145 g          255 t
ORIGIN      
        1 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
       61 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
      121 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
      181 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
      241 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
      301 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
      361 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
      421 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
      481 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
      541 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
      601 tcaattcgtt gttatcgcta ttcaagtagg gcaccgaaga aatgtctatg attaagagac
      661 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
      721 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
      781 taaaaaa
//
LOCUS       DAYYGS010000234          756 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000234 DAYYGS010000000
VERSION     DAYYGS010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.247"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..352)
                     /locus_tag="ACHA44_002947"
     CDS             complement(<1..352)
                     /locus_tag="ACHA44_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906136.1"
                     /translation="MDKPFLDSVREYLSNPKSTLFPHEDYKIIMNYYLPIGAPQNSKI
                     KIPNDIVNDGDTHNVIKGFLPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGL
                     DLKNLLCADDKIKKI"
BASE COUNT          235 a          134 c          110 g          277 t
ORIGIN      
        1 gtattttttt gattttatca tctgcacaaa ggagattttt taaatctaat ccttctttta
       61 tttcaaaagt tgaatttttt aaatctataa gagtaaaacc aattccttca tactgaattt
      121 ctgaaaatcg aatttttctc ccagttgata gatcaagggg cagaaaacct tttattacat
      181 tgtgagtgtc accatcattt acaatgtcat taggaatttt gattttagaa ttctgtggag
      241 cacctattgg aagataataa ttcattataa ttttgtagtc ttcatgagga aataaagtac
      301 ttttaggatt gctaagatat tctcgtaccg aatctaagaa tggcttatcc attgaatctt
      361 catccccatt taaactaaaa aacattccta caattgcttt tgcaactcta agtgaattca
      421 ttttctttat tgaatgtgtt aaatcagact taccattctt taaacttgta ttgttataaa
      481 ggaaattttt attgtcttct gcaaatctaa tatattcctc aacatactca ctaccaaaaa
      541 aactattaca tttactacat aacgtattgg aatggcaaca gtttttttga caaattttat
      601 aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga gcctcctcgg
      661 atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct agcgttagca
      721 catccgagct cattatcaag gattcgctgc gccaat
//
LOCUS       DAYYGS010000235          676 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000235 DAYYGS010000000
VERSION     DAYYGS010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.248"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>676
                     /locus_tag="ACHA44_002948"
     CDS             <1..>676
                     /locus_tag="ACHA44_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-54"
                     /protein_id="HFM8906137.1"
                     /translation="KIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANR
                     IVERYIYDAIDMPLMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDA
                     QKKFGLLNNVSTRMAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCS
                     CASGLGTSFKLKRILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLN
                     PHLEDVE"
BASE COUNT          250 a           87 c          124 g          215 t
ORIGIN      
        1 aaaaattcaa aaaaatgttt tggttatttt atttaagtta atgtgtagta aagaatttca
       61 gcaaaaacgt ttagcactga ttttggctca tggagatcta acggcaacct gtatagcaag
      121 tactgctaat aggatcgttg agagatatat atacgacgcc attgatatgc cactaatgac
      181 ttcaagtagt gagattgcag acaaaattaa tagatactta caagagtgta ccggatatga
      241 agaaattatt ttacttgttg atatgggttc actggagaaa atttatctaa atttggaact
      301 agacgctcaa aaaaaattcg gactactaaa caatgtttca acaagaatgg ctattgatgt
      361 tgcaaatcag atattattag atttaccgct taaacagatt cttgaacaag taaaagaaaa
      421 tcaaaatgtg gattatattt atgaatcatc aaatattaag aaggatatga ttgtctgttc
      481 ttgtgcaagc ggattaggaa cttcgtttaa attaaaaagg atacttgaag atagctttcc
      541 aagggatact gatcttgtca tagaaacgtg tgactattac tctttagtga atgaaaacta
      601 tttatcggag cttcgtaaag agaacaatat tctttttatt gttggaacac taaatccaca
      661 tctagaagat gtagaa
//
LOCUS       DAYYGS010000236          663 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000236 DAYYGS010000000
VERSION     DAYYGS010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.249"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            123..458
                     /locus_tag="ACHA44_002949"
                     /pseudo
     CDS             123..458
                     /locus_tag="ACHA44_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            554..>663
                     /locus_tag="ACHA44_002950"
     CDS             554..>663
                     /locus_tag="ACHA44_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8906138.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
BASE COUNT          234 a          127 c          132 g          170 t
ORIGIN      
        1 ttttatataa taaaatgcaa tacttttcga gagtgtaaaa tattttgtgt aaatagaaaa
       61 aaggaagtcc cttctgtaga atagagttac cacaacacat tcacagaaaa gaggacttcc
      121 atatgaacga ttttactaca gaaattctaa agactctagc gaacaaaggc gatttgaatg
      181 aattattccg tgtccatttg gaaaaagctg tcaatacgct tctcaaaacg gagttaacgg
      241 ctttcctcga ttacgaaaag tacgatcgca ttggttttaa cacgggtaat tctcgtaacg
      301 gctcctatga ccgtacggtc aagaccgagt acggggaact tcatctccag attccgcgcg
      361 accgcaacgg cgagttcaag caacagactg ttcctgctta tagacggacg aatgatacct
      421 tagaggagac cgtcattcac ctcttccgaa aaggtatgga taggctacaa ttaactagtg
      481 gataggctac aattaactag acagaaaaat taaggtgtgt agactagaag aaaacatacc
      541 aggaggaatt tttatgtcta agagaacacg aagaactttt tcacaagaat tcaagcaaca
      601 aatcgtcaat ctttacttag ctggaaagcc acgtgtagaa atcattcgag aatatgaact
      661 aac
//
LOCUS       DAYYGS010000237          649 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000237 DAYYGS010000000
VERSION     DAYYGS010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.250"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(7..582)
                     /locus_tag="ACHA44_002951"
     CDS             complement(7..582)
                     /locus_tag="ACHA44_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytS/YhcK type 5TM receptor domain-containing
                     protein"
                     /protein_id="HFM8906139.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          212 a          142 c          117 g          178 t
ORIGIN      
        1 gtttattcag cctatttttc tttttgaata tatcaagcgt tggggcaaca tagtgatgac
       61 taatgaaaca acgacgatag acaacaagcg atcaaggtag tctgtacccg cctgtaccaa
      121 gatcacactt gttgtctgac ttaaacctaa cccatgtaat agctgaacga tcatactgga
      181 tcctgaggag gtgatcccgc caaataactg aacggtaatg atcgaagaaa cgatcgttcc
      241 aggtaccgac aaaatcaaag cgagtaataa cagtttcaag ctgtttttat ctttgccgag
      301 atatcttggc aaaatcaatc cagcaagcaa gcctgtgaga atttggattg gcgaataata
      361 cagagaaaaa atatctgtag tcactccact taaaataccg ctggacaatc ctgtcagcat
      421 tccaggtaca ggcccaagca atgcaccagc aaaaatcgtt ccgatagaat cgagatagat
      481 tggcaaacga agccataaag caatcgttcc tccaatataa ttgatcccaa tacacaaagc
      541 catcactgta agtacgcgag ttgccatttt gtttttattc attttgttag cccatcttta
      601 acctttccaa atctttcata atcgtttctt cttgtgcatt taaaagaac
//
LOCUS       DAYYGS010000238          632 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000238 DAYYGS010000000
VERSION     DAYYGS010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.251"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..321)
                     /locus_tag="ACHA44_002952"
     CDS             complement(<1..321)
                     /locus_tag="ACHA44_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HFM8906140.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQE"
BASE COUNT          194 a          114 c           83 g          241 t
ORIGIN      
        1 ttcctgaaag aaagccaata ccattttcat agataaagaa tccgttatat aacgagataa
       61 cagagctttg ctagaacatt ttccattaat ttccagacat atatccaatt cactgacggt
      121 tactcgcaaa tttttctcta gatcgaactt ttgtaaatca tgtttcagct tatcgatcgt
      181 ttttttcaag agaaatgtcg atagcgacaa ctcttcttga acattcgata ttgactctga
      241 taaatttgag cttgcttcta aatatttaag taatgaataa cttctgtgat cttctttgct
      301 taaaaaaaac tcataattca ttagtcatca gctctttcta cgtatattac tttccatgca
      361 ttcaccaact agctggggat aaaactgatt tcctggatcc gagtcactgt tgtcagcgat
      421 tcttgtttgt ataatataca tcgtcctgtc ttttttttaa cattttggag agcgttttat
      481 ttggcctttc tcgcgatcaa acctgttatt agaattatat catttactag tatgcatttt
      541 tattaataaa atggtatatt ttatattgtt ataaaaagag cccaaccatc ctacaataat
      601 aaaaatttac tatttgttat cttaacctta ct
//
LOCUS       DAYYGS010000239          601 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000239 DAYYGS010000000
VERSION     DAYYGS010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.252"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..156
                     /locus_tag="ACHA44_002953"
     CDS             <1..156
                     /locus_tag="ACHA44_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFM8906141.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>601
                     /locus_tag="ACHA44_002954"
     CDS             175..>601
                     /locus_tag="ACHA44_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFM8906142.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGK"
BASE COUNT          265 a           47 c          106 g          183 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 a
//
LOCUS       DAYYGS010000240          588 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000240 DAYYGS010000000
VERSION     DAYYGS010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.253"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(65..150)
                     /locus_tag="ACHA44_002955"
     tRNA            complement(65..150)
                     /locus_tag="ACHA44_002955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="ACHA44_002956"
     tRNA            complement(167..238)
                     /locus_tag="ACHA44_002956"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="ACHA44_002957"
     tRNA            complement(250..322)
                     /locus_tag="ACHA44_002957"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="ACHA44_002958"
     tRNA            complement(340..421)
                     /locus_tag="ACHA44_002958"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="ACHA44_002959"
     tRNA            complement(429..501)
                     /locus_tag="ACHA44_002959"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="ACHA44_002960"
     tRNA            complement(505..577)
                     /locus_tag="ACHA44_002960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAYYGS010000241          571 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000241 DAYYGS010000000
VERSION     DAYYGS010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.254"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>571
                     /locus_tag="ACHA44_002961"
     CDS             <1..>571
                     /locus_tag="ACHA44_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tip lysozyme"
                     /protein_id="HFM8906143.1"
                     /translation="DGFKACTDPRQAAYAFERNYERPAATHPERQDYAVNWYNKFKDL
                     KPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYSGYLGGCGMGAGTKYGLTHVIGNT
                     SAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIINWARGGQVGSWFADGTYGHTGVIR
                     GLANGRMQTYEQNTELGMICGKLDRQYYSA"
BASE COUNT          174 a           84 c          147 g          166 t
ORIGIN      
        1 tgatggattt aaggcctgta ctgatccaag acaagcagca tatgcttttg aacgaaacta
       61 tgaacgtccg gcagcgacac atcctgaacg tcaagattat gcagttaact ggtataacaa
      121 atttaaagat ttaaaaccag gaggagctac tggagaagca ggactaaaac atttagagtc
      181 tttgattgga caacgaattg gcaatggtca gtgttatggc ttgtctgcag aatattcagg
      241 atatctgggt ggttgtggca tgggtgctgg aacaaaatat ggtttaactc atgtgatagg
      301 aaatacttct gcagcatctg atattggtat tgcctatgat tggtctgctg ttggttggaa
      361 agtgattcaa aaccctagat atgatcaatt agtagttggt gcaattatta attgggcaag
      421 aggtggacaa gtgggttcat ggtttgcaga tggaacttat ggacatactg gcgttatccg
      481 aggcttagct aatggtcgta tgcaaactta tgaacaaaac acagagttag gtatgatttg
      541 tggaaagtta gatcgccagt attatagtgc a
//
LOCUS       DAYYGS010000242          566 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000242 DAYYGS010000000
VERSION     DAYYGS010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.255"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>566
                     /locus_tag="ACHA44_002962"
     CDS             <1..>566
                     /locus_tag="ACHA44_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906144.1"
                     /translation="FTNLAIGYNLFLYIVTLITLFICHYVMYEITKKHVTSSFFAIIY
                     TTSSFRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAISMTLLIYTHV
                     LSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIGPMIEQTLYQD
                     LNRPYVDILQKRAIYLGKEFLIGSIDSE"
BASE COUNT          181 a           78 c           87 g          220 t
ORIGIN      
        1 tttactaatt tagcaatagg ctataacctt tttctttata ttgttacgct cattactcta
       61 tttatatgtc attatgtaat gtatgaaatt accaaaaaac atgttactag ctcctttttc
      121 gctattatat atacaacatc ttcttttagg agtgtagaaa tatttcttcg aggggcaatg
      181 ggggaattac tcgcaatgtc aatacttcct cttattttac tcgggtttat aaagctttac
      241 gactcaaaaa aagaatcttg ggttatgttg gcgattagca tgactttatt aatttacact
      301 catgttcttt ctgtagctat gactatggta atgattataa ttgctttaat tattcaattt
      361 tatcggaaaa agaaaataga aatcttctta attattaaat taataaaagc tgctatagtt
      421 actttacttc tttcactagc gtttataggt cctatgattg agcagacatt gtatcaagat
      481 ctgaatagac cttatgtgga tatattacaa aaaagagcaa tttatctggg taaagagttt
      541 ttgattggaa gtatagattc agaatt
//
LOCUS       DAYYGS010000243          544 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000243 DAYYGS010000000
VERSION     DAYYGS010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.256"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>544
                     /locus_tag="ACHA44_002963"
     CDS             <1..>544
                     /locus_tag="ACHA44_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HFM8906145.1"
                     /translation="RFHIIKHMNQAFNELRIREMNELRKVGQKSQAEKLKKNWRFLLK
                     NRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAYPYFHELVEAFRDKD
                     PDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNGKIEAKNTHIKTMKR
                     VSYGFKSFENMRIRIFLINQL"
BASE COUNT          192 a          106 c          100 g          146 t
ORIGIN      
        1 tcggtttcat attatcaaac acatgaatca agcctttaat gagttgcgta tccgtgaaat
       61 gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg
      121 ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa tacaaaacat ggaaaagttt
      181 tcgagcacca aaatacccat ttcttacgga agcgatgatg attgatcgat tgcttggctt
      241 ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa ttagttgaag cctttcgaga
      301 caaagaccct gacttatttt tctccttatt ggcagaactt cccgaaacgt tagatgacgg
      361 ctttcgggaa aagctccaaa accttctgac ctatgaagaa ggcatcacca acgcgatgat
      421 ctatccttac accaatggaa aaatagaagc gaagaatacc catataaaga caatgaaacg
      481 agtatcctac ggatttaaat catttgagaa catgagaatt agaatctttt tgatcaatca
      541 atta
//
LOCUS       DAYYGS010000244          536 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000244 DAYYGS010000000
VERSION     DAYYGS010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.257"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(145..444)
                     /locus_tag="ACHA44_002964"
     CDS             complement(145..444)
                     /locus_tag="ACHA44_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFM8906146.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT          174 a          100 c           63 g          199 t
ORIGIN      
        1 aattaaagga taaaatacaa tataaagaaa catctgtttc tttatttaga ataaaaaaat
       61 acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa
      121 ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat
      181 tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga
      241 tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt
      301 tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat
      361 acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt
      421 ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta
      481 cattctataa acaaagtata cgctacatct agattttttt aaatcattac tggatt
//
LOCUS       DAYYGS010000245          524 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000245 DAYYGS010000000
VERSION     DAYYGS010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.258"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(37..>524)
                     /locus_tag="ACHA44_002965"
     CDS             complement(37..>524)
                     /locus_tag="ACHA44_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906147.1"
                     /translation="SFFRENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLI
                     NHIPIQLISEPVKIIIDFSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNR
                     NNLYEESASQVVVWLDPPRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRI
                     E"
BASE COUNT          167 a           92 c           84 g          181 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaa
//
LOCUS       DAYYGS010000246          518 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000246 DAYYGS010000000
VERSION     DAYYGS010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.259"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(125..196)
                     /locus_tag="ACHA44_002966"
     tRNA            complement(125..196)
                     /locus_tag="ACHA44_002966"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(161..163),aa:Glu,seq:ttc)
     gene            complement(208..281)
                     /locus_tag="ACHA44_002967"
     tRNA            complement(208..281)
                     /locus_tag="ACHA44_002967"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Ile,seq:gat)
     gene            complement(294..364)
                     /locus_tag="ACHA44_002968"
     tRNA            complement(294..364)
                     /locus_tag="ACHA44_002968"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(330..332),aa:Gly,seq:tcc)
     gene            complement(369..441)
                     /locus_tag="ACHA44_002969"
     tRNA            complement(369..441)
                     /locus_tag="ACHA44_002969"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Phe,seq:gaa)
     gene            complement(445..517)
                     /locus_tag="ACHA44_002970"
     tRNA            complement(445..517)
                     /locus_tag="ACHA44_002970"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Asp,seq:gtc)
BASE COUNT          167 a          137 c          107 g          107 t
ORIGIN      
        1 aaatatttta cattagaact gtccaaaaaa gcttagcaca atcatccata aagataactc
       61 tgctaagctt tttaaaatca ttctttcaga taaaaagaaa aagatactca aaaagtatct
      121 tccatgaccc gtacgggatt cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc
      181 gcttgaccaa cgggccaata aaataaatgg gcctaaatgg actcgaacca tcgacctcac
      241 gcttatcagg cgtgcgctct aaccagctga gctataggcc cataaaaact aaaagcgggt
      301 gacgagaatc gaactcgcga caacagcttg gaaggctgtg gttttaccac taaactacac
      361 ccgcataatg gcgcgggaca gaatcgaact gccgacacat ggagcttcaa tccattgctc
      421 taccaactga gctaccgagc caaacggttc cgacgggaat cgaacccgcg atctcctgcg
      481 tgacaggcag gcatgttaac ccctacacca cggaacca
//
LOCUS       DAYYGS010000247          509 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000247 DAYYGS010000000
VERSION     DAYYGS010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.260"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..132)
                     /locus_tag="ACHA44_002971"
     CDS             complement(<1..132)
                     /locus_tag="ACHA44_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFM8906148.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DAYYGS010000248          498 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000248 DAYYGS010000000
VERSION     DAYYGS010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.261"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            102..296
                     /locus_tag="ACHA44_002972"
     CDS             102..296
                     /locus_tag="ACHA44_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906149.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
BASE COUNT          209 a           65 c           83 g          141 t
ORIGIN      
        1 aattgattct ttttatttat cataaagata tagatacata atcattttca cttacatata
       61 tttgaatatt aactaaaaac agcaagaaag aagaggatca aatgtattat gtagaagtaa
      121 aaacaaaagg cgtcaaaaac aaacaatatg tgaaaggtat cagtaacgaa tatcctttac
      181 ttggttcatg gaaagaagct gctccttttt ccaaaccatg tgccataaaa ataaagaacg
      241 agctagaaaa agaactgact tgtggtaaag ctgttgtaga tatcatcgaa aagtaaatcc
      301 aatgtataga aaggaagatc acacatgaaa agcgacgtac aaattgctaa gattatagaa
      361 ttagaagaag aagacgcagt gttgacaatt gaaaaaaaat aaatgtttgt ataccatatg
      421 attatttcaa tatgtatcct ggaattggag atctcgtaga gctatatcaa gatgaatcaa
      481 atatacttgt cattcatc
//
LOCUS       DAYYGS010000249          479 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000249 DAYYGS010000000
VERSION     DAYYGS010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.264"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..430)
                     /locus_tag="ACHA44_002973"
     CDS             complement(<1..430)
                     /locus_tag="ACHA44_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8906150.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          135 a           95 c           64 g          185 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tctaactttt ggggtgcac
//
LOCUS       DAYYGS010000250          468 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000250 DAYYGS010000000
VERSION     DAYYGS010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.265"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>468
                     /locus_tag="ACHA44_002974"
     CDS             <1..>468
                     /locus_tag="ACHA44_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HFM8906151.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHK"
BASE COUNT          155 a           86 c           83 g          144 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaa
//
LOCUS       DAYYGS010000251          451 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000251 DAYYGS010000000
VERSION     DAYYGS010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.266"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>451
                     /locus_tag="ACHA44_002975"
     CDS             <1..>451
                     /locus_tag="ACHA44_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rib/alpha-like domain-containing protein"
                     /protein_id="HFM8906152.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           97 t
ORIGIN      
        1 ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca gaagataaac
       61 ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag gaagcaactc
      121 aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg gatgataaat
      181 ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg aataaggaac
      241 cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact aagtatactt
      301 ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt gtagtgacat
      361 atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac aatcgttctg
      421 acgccgataa atatgagcca acagtagaag g
//
LOCUS       DAYYGS010000252          438 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000252 DAYYGS010000000
VERSION     DAYYGS010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.267"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            8..>438
                     /locus_tag="ACHA44_002976"
     CDS             8..>438
                     /locus_tag="ACHA44_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8906153.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEIL"
BASE COUNT          179 a           70 c           67 g          122 t
ORIGIN      
        1 aaaaaagatg aagccaaatc cttcagaagc aggaaaacga atcaaacaat tacgcctttc
       61 atgtggtttt actatggaag aacttggaag gaaaatagat aattcaccac gtgcgaccat
      121 atccaactgg gaacgtggga ccaatttacc aaatccacaa aagctgaaat tattatcaac
      181 tatcactaat tcaacaattg actggattaa atggggaact ttagaagaat atataacttc
      241 ataccttatc gatatagggt atgagctata tattaaggac ttcccagaaa tacctcacaa
      301 agtctttaaa gacattcaag aaagatatag tgatacgttt tcgctagata aagactatga
      361 gttattgaat cctataataa aaaatatatt tacaaaatat tactcaaaag attttgaaat
      421 ttatctaaat gagatact
//
LOCUS       DAYYGS010000253          414 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000253 DAYYGS010000000
VERSION     DAYYGS010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.268"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(109..>414)
                     /locus_tag="ACHA44_002977"
     CDS             complement(109..>414)
                     /locus_tag="ACHA44_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFM8906154.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          116 a           96 c           82 g          120 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggt
//
LOCUS       DAYYGS010000254          406 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000254 DAYYGS010000000
VERSION     DAYYGS010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.269"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(38..376)
                     /locus_tag="ACHA44_002978"
     CDS             complement(38..376)
                     /locus_tag="ACHA44_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HFM8906155.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT          129 a           80 c           47 g          150 t
ORIGIN      
        1 aaatgtcaaa ttccgttctc aactgtgcat attcattcta tgttccatgt accccattat
       61 cctcctgaat actcattcca atgttagtta atttttctaa ttcttctacg cttagataag
      121 cgccatattt tacaatttcc tcatcaatga caataatcgg taaacttttc aaatcttttg
      181 tctgcaacaa gttaagaatt ttagggttgc taataaaagg caagatagag tcacttaaaa
      241 aatataaatt tgtttctatc tgtttattct taatccgatt ataaaactct tttagcatcg
      301 tatcctcatg taatgattga aaaacgaaga gtccttcatc atcacttcta tttaagacat
      361 agtaagaaat tatcattttc ctattgtcaa tcctcccgca gtgcaa
//
LOCUS       DAYYGS010000255          392 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000255 DAYYGS010000000
VERSION     DAYYGS010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.270"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          127 a           53 c           57 g          155 t
ORIGIN      
        1 agatattttc aaaacatttt ttcttgatgt gatatccttg tcttttttgt aatgttttta
       61 acttattgct tttgcaaaat ttatttacct gttttctgga tcaacgatag tgtatattat
      121 gctatccttg tttggatttt aggacgttta aagaggcact tttttacata acttaatcca
      181 tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg caccccaaac
      241 cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca ctaagaagca
      301 taccatattt ttgaaaaaaa gtgagaaaaa aggcttataa ttaggattta gagtaattaa
      361 ttagatgttt attttatagg actgatacta ta
//
LOCUS       DAYYGS010000256          362 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000256 DAYYGS010000000
VERSION     DAYYGS010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.271"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..355
                     /locus_tag="ACHA44_002979"
     CDS             <1..355
                     /locus_tag="ACHA44_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HFM8906156.1"
                     /translation="KFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSK
                     GVVFDRLESIYNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSN
                     DKDGVSQAFERILTI"
BASE COUNT          130 a           48 c           61 g          123 t
ORIGIN      
        1 caaatttttt atttttattc ctaatcgtca attgaaagct aatatattta accatttgaa
       61 aaagatgcta aatgtgtctg ttactgaatc ggcacctgta aatattgaaa tcgtacctca
      121 aaatgtatct aaaggtgtgg tgtttgatcg tttggaatca atctacaatt taaagaaacc
      181 tttgagaatc gcgatcggag atagtttaaa tgacttatct atgtttgaaa gtgcagatat
      241 tagttttgca atgggaaata gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt
      301 ttcaaatgat aaagacggag tatctcaagc atttgaaaga attcttacca tataaactaa
      361 tt
//
LOCUS       DAYYGS010000257          339 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000257 DAYYGS010000000
VERSION     DAYYGS010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.272"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..318
                     /locus_tag="ACHA44_002980"
     CDS             <1..318
                     /locus_tag="ACHA44_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8906157.1"
                     /translation="ANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYS
                     TNLIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLE
                     SMF"
BASE COUNT          135 a           50 c           49 g          105 t
ORIGIN      
        1 gcaaataatc aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt
       61 aaattgctca tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga
      121 actatttatt caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa
      181 agaaaagagc aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac
      241 aactataatc aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca
      301 ttagaatcaa tgttttaact tgatcaacag aatgggctt
//
LOCUS       DAYYGS010000258          321 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000258 DAYYGS010000000
VERSION     DAYYGS010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.273"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..>321)
                     /locus_tag="ACHA44_002981"
     CDS             complement(<1..>321)
                     /locus_tag="ACHA44_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8906158.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAYYGS010000259          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000259 DAYYGS010000000
VERSION     DAYYGS010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.276"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            1..309
                     /locus_tag="ACHA44_002982"
                     /pseudo
     CDS             1..309
                     /locus_tag="ACHA44_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          122 a           61 c           59 g           67 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaa
//
LOCUS       DAYYGS010000260          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000260 DAYYGS010000000
VERSION     DAYYGS010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.277"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            161..271
                     /locus_tag="ACHA44_002983"
                     /pseudo
     CDS             161..271
                     /locus_tag="ACHA44_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          104 a           51 c           39 g          115 t
ORIGIN      
        1 catctttcag cattttacta tatttggtat atagtgctaa ggttcatatt cgattgacaa
       61 gagaatataa attgatctat ttctatttcc taagttgagt aaacataact ttgcccttta
      121 taaatatctt ccctctctct cttctctcta tattttatat ttgaataatc atatcaaggt
      181 attccaacga aatgccaatg gatttcgaag cttttataac tttaaattac ggatcatgat
      241 ttggcatggg gaatcattac taataaaata aaaaaatgaa cagaaagaaa acagttcttt
      301 ctgttcacc
//
LOCUS       DAYYGS010000261          309 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000261 DAYYGS010000000
VERSION     DAYYGS010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.278"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
BASE COUNT          111 a           57 c           26 g          115 t
ORIGIN      
        1 ctgaattcgg aattaaaaac cacgataggt aactttaaac aaacatgaag agactttaat
       61 aagacttctt tatctgaaaa cacttttgtt tctctctttc ctttctacat cattaaatag
      121 tattttatac gttatctaaa atactattcc tctgtcaaaa taagctaact tacttcatta
      181 taaaatatcc acttatcgct ttcatgtata ttccaccgta aaaagtacat tttatcaccg
      241 agaactcatc atttttcatc atgatgaata ctatttacat atatctagtt caaagacaat
      301 aattattat
//
LOCUS       DAYYGS010000262          288 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000262 DAYYGS010000000
VERSION     DAYYGS010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.280"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            11..>288
                     /locus_tag="ACHA44_002984"
     CDS             11..>288
                     /locus_tag="ACHA44_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906159.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTV"
BASE COUNT          115 a           31 c           53 g           89 t
ORIGIN      
        1 gtaaaaggac atgatgatta tggaagaaat agaatggaat aatctgactc aaaataataa
       61 aaataattct ggatgtggaa ttatctttat gtttgttata gttttgggaa tagtagtact
      121 tttaataact gtcaagatag tagattactt tgcgaatact cctgaagaac aaatacagag
      181 aaaacttcct gagtttgaaa gagtaccacc ttatgatcca agagataatt catttcaagt
      241 aaaaaaatta aaagagtctc caaatgaaat gtggtttgaa gttacagt
//
LOCUS       DAYYGS010000263          282 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000263 DAYYGS010000000
VERSION     DAYYGS010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.denovo.282"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            <1..>282
                     /locus_tag="ACHA44_002985"
     CDS             <1..>282
                     /locus_tag="ACHA44_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFM8906160.1"
                     /translation="QSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPKGK
                     KGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           84 a           52 c           56 g           90 t
ORIGIN      
        1 aacaatcgaa gagacttttt cctgaaatca attgtgttgt cgctgatgct gggtataaga
       61 cacctaagtt tgttcacttt ttgactcatt tgaaccttcg accttgtttg ccttattcaa
      121 ggcccaaagg gaaaaagggg ctattatcaa aaaatgagtt tctctatgac gagtattttg
      181 attgttatat ttgtccgcaa gatcaaatgc tcgctttttc aacggttact cgagaagggt
      241 accgagaata caaatccaat ccaaaagaat gtgtgaactg tc
//
LOCUS       DAYYGS010000264         8139 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.099, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000264 DAYYGS010000000
VERSION     DAYYGS010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.099"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(134..577)
                     /locus_tag="ACHA44_002986"
     CDS             complement(134..577)
                     /locus_tag="ACHA44_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HFM8906161.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="ACHA44_002987"
     CDS             complement(795..2135)
                     /locus_tag="ACHA44_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFM8906162.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="ACHA44_002988"
     CDS             2404..3300
                     /locus_tag="ACHA44_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HFM8906163.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="ACHA44_002989"
     CDS             complement(3450..4391)
                     /locus_tag="ACHA44_002989"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFM8906164.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="ACHA44_002990"
     CDS             4517..5638
                     /locus_tag="ACHA44_002990"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFM8906165.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA44_002991"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="ACHA44_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection-like protein
                     Eat(A)"
                     /protein_id="HFM8906166.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="ACHA44_002992"
     CDS             7465..7992
                     /locus_tag="ACHA44_002992"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HFM8906167.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
BASE COUNT         2704 a         1511 c         1603 g         2321 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaattt
//
LOCUS       DAYYGS010000265         7959 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.103, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000265 DAYYGS010000000
VERSION     DAYYGS010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.103"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            128..1564
                     /locus_tag="ACHA44_002993"
     CDS             128..1564
                     /locus_tag="ACHA44_002993"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HFM8906168.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="ACHA44_002994"
     CDS             1753..4200
                     /locus_tag="ACHA44_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFM8906169.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA44_002995"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="ACHA44_002995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFM8906170.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            5487..5693
                     /locus_tag="ACHA44_002996"
     CDS             5487..5693
                     /locus_tag="ACHA44_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906171.1"
                     /translation="MPNNRILLVFSITIKGAVFLIKELFNLNKRKDSIAKAIKLFLTR
                     KTLIKQRNVLNLMTACLMGVLAII"
     gene            complement(5690..6793)
                     /locus_tag="ACHA44_002997"
     CDS             complement(5690..6793)
                     /locus_tag="ACHA44_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HFM8906172.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA44_002998"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="ACHA44_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HFM8906173.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DAYYGS010000266         7612 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.108, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000266 DAYYGS010000000
VERSION     DAYYGS010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.108"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..347)
                     /locus_tag="ACHA44_002999"
     CDS             complement(<1..347)
                     /locus_tag="ACHA44_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HFM8906174.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLII"
     gene            complement(552..1745)
                     /locus_tag="ACHA44_003000"
     CDS             complement(552..1745)
                     /locus_tag="ACHA44_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFM8906175.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(1824..2030)
                     /locus_tag="ACHA44_003001"
     CDS             complement(1824..2030)
                     /locus_tag="ACHA44_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFM8906176.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(2023..3165)
                     /locus_tag="ACHA44_003002"
     CDS             complement(2023..3165)
                     /locus_tag="ACHA44_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFM8906177.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            3677..4312
                     /locus_tag="ACHA44_003003"
     CDS             3677..4312
                     /locus_tag="ACHA44_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFM8906178.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            4448..4540
                     /locus_tag="ACHA44_003004"
                     /pseudo
     CDS             4448..4540
                     /locus_tag="ACHA44_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323349.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            4654..5289
                     /locus_tag="ACHA44_003005"
     CDS             4654..5289
                     /locus_tag="ACHA44_003005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFM8906179.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            5396..6634
                     /gene="mef(H)"
                     /locus_tag="ACHA44_003006"
                     /gene_synonym="mefH"
     CDS             5396..6634
                     /gene="mef(H)"
                     /locus_tag="ACHA44_003006"
                     /gene_synonym="mefH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_070509550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide efflux MFS transporter Mef(H)"
                     /protein_id="HFM8906180.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            6861..7280
                     /locus_tag="ACHA44_003007"
     CDS             6861..7280
                     /locus_tag="ACHA44_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HFM8906181.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            7285..7524
                     /locus_tag="ACHA44_003008"
     CDS             7285..7524
                     /locus_tag="ACHA44_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFM8906182.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
BASE COUNT         2293 a         1332 c         1434 g         2553 t
ORIGIN      
        1 taaataataa gagaagcaat caagatccct atactttttt gtttaggtgt cagctcatat
       61 ttatcatcga gcactcctgt gactatgatt actaaagaag caatcaagat cggtacaata
      121 tacgattgag ggataatttt acggaataag aataaagtcg atataacaaa tgaaataaag
      181 ataggcagtc cgcccgctga aggcattgga actttattta ttcttctttc atttggtttg
      241 tcgaccatac ctgtttgtac tgaaatgatt ttgaatacag gtgttaatat cagtgataat
      301 aaaattgtca gaaaaaatct tataaccata ctaaaagaaa caagcatatg ggcacctact
      361 ttaattaaga gtcataatta ttataaatta tatatgtctt tttgactatc tttttatatc
      421 ttctcaaatg tccgcaaagc cttattttat cggctctacg ggcattttgc ttttgtggta
      481 aacctcgcat atctaggtct atcttcttat attttccctg tcaatcgtgg ttaaaatcgt
      541 ggtaaatact tctatgcgat tagacgctga acctctgatt ttgcggtatc tatggacgca
      601 tgagcgtacc agttcattgt gatactgatg tttgaatgcc ccatgatata ctgtaaatct
      661 ttcgggttca tgttcttgct tgccagcctt gtgcagaatg tatggcgtag cgtatgcggt
      721 gtaatatgtg gcaagggatt gtccttgtgg tgcttgttgt atttctttac catacggata
      781 aataaggcgt tataatcaat ggcaactttt ggattacctt tcggattgat aaacacaaaa
      841 ccgctgtgtc cgtctacctc tatcgacttt atctttggtc ggctgttgat aacccgttga
      901 aatgcctgta tcgtttcttt gcttaatggc acttgcctta tcccgctttt cgtcttaggc
      961 gtttcaatat aatagccctg ttccttactc tttagtaact ggtggtcgat aatcacaact
     1021 tcattcttga aatcaatgtc ggctactgtc agtccgcaca gttccgagat acgaagtcct
     1081 gtctttaaca gtatcagcac atcatcataa tgcttgtgat acacgttgtc cgtcttaata
     1141 aatgacaata aggcttgctc ctgttcctct gtcaatgcga ctttctcttt ggtatcattt
     1201 tctatgactt cacttagctt gaaatcaaaa gggtttttcc tcacataatc atcttgtatg
     1261 gctatgtaga atgacgcttt taacgagcgt ttatggttgt taatggtatt gtaggaaaag
     1321 cctttgtctt tcatgcgtaa cgcccattct ttagcgtcag agggttttat cgtatcaatg
     1381 ctcctagcac ctaacttgtc ctcttttaat aaccgcatga gttgttcccg ttgtttctgt
     1441 gtgcttttct tcacatttgt cctctgtgcg ttctgtttgg catagagttg gcaaagcgtc
     1501 attttcttgc ctgtgctgtc gataccgtcc tcaatatccc gtcttatctg ctgttccagt
     1561 tctcgaagtg agggtttttc ccgttttccc tttggtgtcg ggtctgtggg tgtcaatctc
     1621 caagcataca catattttgt gtttcccaat gcgtctacat atttatataa gtattttccg
     1681 tctgttcgtt ggctctctcc agtatgcagg atacgacctt tgttatcccg tcttttttct
     1741 ttcatggtgt ctgctccttt ccttgttgga aagagccttg atatgacttg tgttcatcat
     1801 aacacataca aggctcattt gcattagatt gcgtccaatt tatcaataac cttttcaaac
     1861 tgtcggcgtt taatctgtat gcggttgcca ttcataatga gccagccagc gtccttgttt
     1921 tcctctgcca atcgtcttaa cttgttttct ccaatgcgga aatactttga cgcttcttca
     1981 atggtaaggg tgtatttttc ccatacggga atatcctgtg tattactcat aaaacacccc
     2041 ctcactttcg attacgtctg atatgtccgt acacatctgt tccacaagca tttcctgttt
     2101 ttcagaaagg tgggtacatt catcaatcat tttcatgtag tttgaacctg tcagcctgtc
     2161 gatttcctct atcatcacga ttgacggcat gacctgtctt tccagccatt gcagagtgcg
     2221 ttccatattg acaggtacag ggttcatttc aaaaggtaaa ctgtcatggg aaatgaatat
     2281 ctcccacaat ggatagtcag gaaattccac aaaatcggta atgagataaa acaccagccc
     2341 atgagggtta tacgtcaata acagttcttc aactgcctgt accgcctttt tgtcacgcag
     2401 acgaatttca aaacggttga taatatcggt atgtttcttt ttcgttgcct gttccttttg
     2461 tttttcatac aggcagaaat attttgtgct tgcctttgag ccgatataaa gggtacttgc
     2521 taaattacgg ttttttccgc ttaattttcc accttgtaca ttttcatagt ttttacagcg
     2581 acattccgca ccaccatttc tgtatttttc agatagcaca ggaatatcca gcagaccgca
     2641 catatcattg attgccaagt caaaacgtcg gataacaccg ccacagtcaa gacagcggtt
     2701 taagaatgaa taccagtccc tgttctgtgc ctgtaagaca tattccatgt tgcgtgagcc
     2761 tgcccctttt aattctaaaa atacgcccat gtgttcatca tcagaagcca tgaccttgat
     2821 ttccccgtaa gcataatgct ccttatagcc ataataccca tagtcataat ggataaaata
     2881 atctgctttc atgccaagca cctttcggat aacttccaat gcgtcagtcg ttggaaaacg
     2941 gacagacaga taatcaacga gcagataaaa aggcttatcg cagatatagt aattcagaca
     3001 acgcaggata atgttctgtg tttccttgct ggcgttagct tttccgttct caaagcggtt
     3061 gatactatat cttgatacat gggtaagact tgcaagttct ccttgtgaaa gaccgctggc
     3121 aagacgtttg ttcttcattt caagtgcaaa ttctaaattg ttcattgtat tcctccaatc
     3181 ccacaatagc cttgaaatac ctgtaaacca cctttcaaaa agtgtgatat gtactatctt
     3241 cttaaaacct ctcattacta ctcacaggtt tgggggtgta tggtttttga cttgtctttt
     3301 gtgaaatagt gcccccctgt tagataacgg gggcacagac aaggctcgca ggctcgccag
     3361 ccgacaaagc atttcttccc cttgacacaa ggtgtcatgg cggtaagcct gagaggcttc
     3421 cgccaccgtg tcagaaatac tttgtcagca agttctgttt tgccttttct aacaagttcc
     3481 atttcaaccg atattcatat ctacggattt aatcagatag gtatttcttt ttgaatgttt
     3541 ttatatttca ttctattgtg cttattcgat tacaagaaca tagtaattct tatatgctta
     3601 tttgtcaata tctttttttg cctttacaaa tcatattaag catgatagac ttataatagc
     3661 gaagatataa cgagatatgg agggttatgc aatgaaaata agccatttag gaaataatat
     3721 acagaccata agaaaattta ggggaatgaa acaacaggaa cttgcggaca aaatcggtat
     3781 caatatgcag agcctttcca agattgaaag agggttgaac tatcctgcct atgaaacgct
     3841 ggaaaagata atggaagtgc tggacgttac gcccaatgaa ttgttgtcgg gagaatggaa
     3901 gtatgtcaat caatctgaaa aggaagtctg tcagttttta agagccgaag aacgtctaaa
     3961 tgcagaactg aaacatggac actatgataa cttctttgac agcgaggaag aatggctgga
     4021 atatgagtta gaaaagttac gggaatatat tacagactat atcaacggga aaagcattga
     4081 agcgtccgac ctttacccaa tcaaagaatt tatccagcat ttgaagtttc aaaaattatt
     4141 agaccgctat gatgatttat acagtatgga tatgtttggg gaaagcatag agggacataa
     4201 gtataagaca ccttatcaag tcgtgaaaat gataaacccg aactcaaaag aagatatgga
     4261 actgttacga gaagtattaa ggaataacaa ttttgatgat gaggacgagt aacttttttc
     4321 ctcttgcgag aagatacagc aaacaaaaag cccgatcaag agtgcaaagc accaccaatg
     4381 gtggcaaagc tcttgacagg gctttttctt ttctgttgtg ggcaatcaag aggaagaaag
     4441 ggataaaatc tgatttgtgg taaatcaggc aaaggcaacg caggtatcac agtatgaatt
     4501 gacaccgcat aaggaaagca actggaagat tgtagaataa atgaaaaagg cttgcattct
     4561 gcatttttat aaaattgcaa gtctttatat tgacaagcat aaaatactaa gatatacttt
     4621 aatcagacca acggtttgta ttggaggtaa gttatggcac gaaataaata tccagaagaa
     4681 acagtaaaac tcattttaga tgtttctaca aaaatgtttt gtgaaaaagg atatgataac
     4741 acatcattgc aagatattat caatgaaaca aaactttcta aaggtgctat ttatcatcat
     4801 ttcgattcaa aggaagatat tttaaaagca atttttcaaa gaattggaaa tgaaaatgca
     4861 gtaatatttg ctaaaattag agataacgat agtttaaacg gtttagaaaa gttaagagaa
     4921 atatttaaga cagcggtatt caattctaac caaagcgttt tattgacagt ttctcctcaa
     4981 ctattaaaca atcctcgttt tcttgctatg caaatagaac aaatgtatga aattgtagta
     5041 cctgaatttg ttgaacccgt tttagaacaa ggtatcaagg ataagtcttt ggagattgaa
     5101 aattcacatg aaattgcaga agttatcatg gtattaagta atgtatggct caacccatta
     5161 gttgaaatga cggataaaga gggtatggaa aaacgttgca atacattcaa tgccctttta
     5221 aatggaatag gcatagacac attattagac gaagaaacaa tttctgcatt tatacagttt
     5281 tgtaaataac tgtatttgtt atccctatac gatagactgc tctataaaag agcagtttat
     5341 ttaaaccatt atataaaccg acggtctgtt tgattaaata aaaaggagag attttatgca
     5401 agcagaaaaa aaacttttta atcgtgattt tatacttgtt gttataggac agataatttc
     5461 tttatttgga aacgctatcc tacgatttgc attaccgtac tatttgttaa aagaaactgg
     5521 ttcatcagct ttgtttggta ttgctacagc ctgctctttt ttacctatgg ttattttgtc
     5581 gctggtaggt ggtattattg ctgacaggac aaataaaaga aacattatgg tcatactaga
     5641 ttttgtaaca gcaatcataa ttttagtatt ttatttgtca ttgggaaaaa ttcctatcat
     5701 acctttgttt attgttgctt tgatgttact ttatggtatt tctggtgcat atcaaccagc
     5761 ggtacaagca agtattcctc tattggtatc atcagataaa cttaccgtcg gtaatgctat
     5821 aataaatcaa gtaaatactt tagcaaactt tatcggacca gctataagcg gagtagtttt
     5881 tcaattttat ggtatcaatc ccattttaat tgttagtatt atatgtttta ctttttcagc
     5941 cattatggaa atttttatcc atattccaca ccaaaaaaga cctcgtgaag taggtgtaat
     6001 tgcagttgct aaaaacgatt taaaagatag ttataccttt ttgaaaaagg agaaacccat
     6061 ttttatatcc gtaatctttt tggtttgcat tttcaatttg gttttaagtg ctgttttaat
     6121 tgttggtctg cctatcttaa tttctgaagt tttaaaaatg ccagatacac tatatggttt
     6181 ttcacaatca gcattatcat taggtggttt atgtggtggt attttaacag ctcttgttgc
     6241 ttcaaagtta aaactaaaaa acctttatac cttgttactt atttgttctg tttctgtttt
     6301 acttatggga atttcgctat ttttagaatt gcctgcaata gtttcctact gggtagttac
     6361 agtgatgtgc tttgtggcta tgggtgtatc tactatgttt gttgttcaaa tttacactct
     6421 tgttcagaca caaacaccac cgcaattagt aggaaagata atggctacgc tggtagcagt
     6481 tgctatgtgc ggtcaaccta ttggacaaat ggtttacggt attttattcg atgtatttat
     6541 acaagaaaca tgggttgtta tgtttgtagc ttctattgct tcaatcatga tttcaatata
     6601 ttcaaaaaga atttttcaaa agttagagat ataatacaag gggttaaata gtatgttagg
     6661 ctaaaaacaa tatataaaag aaaatttgaa atctgctaaa aggatagtgt tataaaagaa
     6721 agagtaatat tgcagtattt aaggctcgca caaatatatg ttttgtgtgg gcttttttct
     6781 taccattttt ctgtatttct aaagggtata aacggtattc cgtttatata tttcagcatt
     6841 agcagaaagg gggtgagatt atgaggacat cttctttcga gcatatcgtc agattacagt
     6901 tcaattcttt aatgctgatt gtcattaaga acaaagtgaa aagtcgaaac agacagcttg
     6961 caagacattc taaacgtgaa gtattgtttt gtgaaatagc cgaaacgaaa caagttgaac
     7021 gtggaacgat tgatacctgt ttttatgatt gtgtatcatt taaagtcttg cactttacaa
     7081 tttatgtatc tgatgaaaca cttggtacag ttttacatag actgtcagaa aaacagcgta
     7141 gtgcgatttt attacgttat tttcaaggaa tgaacgacag agaaatttca gaactgtacc
     7201 atgtatcacg ttctgctatt tgcagtagaa gaactagagg actaaggaag ttaaaaatgc
     7261 ttttgagcga aaggaagtaa agaaatgagt aaagattatg gctatccctc ttttgaattg
     7321 atttgcaaag cgtcaagcgg agatgaaatg gcagttaaag agattttgaa gttttatgac
     7381 ggttatattt ctaaattatg cttgcgtcct ttttaccact ctgaaagtgg taaaatcatt
     7441 atgcaggttg atgaagaact aaaaggagaa attcatacag acatgatgaa agcaatattg
     7501 aaatttgaaa tcggagtgaa gtaatcaact gtataaaaaa atcgggagat acacagtttc
     7561 gtgctatctc ccttagtagt tttacggtca tatcaaggct cattcaatcg tg
//
LOCUS       DAYYGS010000267         6008 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.122, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000267 DAYYGS010000000
VERSION     DAYYGS010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.122"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            complement(<1..1221)
                     /locus_tag="ACHA44_003009"
     CDS             complement(<1..1221)
                     /locus_tag="ACHA44_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HFM8906183.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAY"
     gene            1500..2306
                     /locus_tag="ACHA44_003010"
     CDS             1500..2306
                     /locus_tag="ACHA44_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
                     /protein_id="HFM8906184.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGWR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            2324..3031
                     /locus_tag="ACHA44_003011"
     CDS             2324..3031
                     /locus_tag="ACHA44_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HFM8906185.1"
                     /translation="MDRKRLTQRFPFLLPMRRAQRKMCFYAGMRFDGCRYAQTIGEKS
                     LSHLLFETDCALYNHNTGFDMIYQENKVFNLKLAAKTLNGLLIRPGETFSFWLFLHTP
                     LTIIQRRGHEVKEFPEPNSDEIKGVDATISEGWIDLKVRNDTDCTYQIWVTLDDEKII
                     GQVFADKEPQALYKITNGSIQYVRESGGIYEYAQVKRMQVALGTGEIIDCKLLYTNKC
                     KICYPLPESVDIQEENQ"
     gene            3028..3999
                     /locus_tag="ACHA44_003012"
     CDS             3028..3999
                     /locus_tag="ACHA44_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HFM8906186.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVSHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTGRGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            3992..5020
                     /gene="vanB"
                     /locus_tag="ACHA44_003013"
     CDS             3992..5020
                     /gene="vanB"
                     /locus_tag="ACHA44_003013"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HFM8906187.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKAAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQMIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVPADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTLPGFTSYSRYPRMMAAAGITLPALIDS
                     LITLALKR"
     gene            5026..5634
                     /locus_tag="ACHA44_003014"
     CDS             5026..5634
                     /locus_tag="ACHA44_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HFM8906188.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
     gene            complement(5700..5873)
                     /locus_tag="ACHA44_003015"
     CDS             complement(5700..5873)
                     /locus_tag="ACHA44_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906189.1"
                     /translation="MLPEKNGGLDFSKPPPAKYKMGVGKVCPTAVFFFSAAGQIEYYN
                     LQCITKDALTTFQ"
BASE COUNT         1701 a         1231 c         1512 g         1564 t
ORIGIN      
        1 ataagcgact cgtttcaatg ctttgatgtg ggtatgcaga ttttctattt tggcatttga
       61 ataaggcaat tcgagggcta aacgaatgcc ggattcatag tttataaaag ctttttttat
      121 agaatgcagt tcttttggaa cggaattggg catcgttctt acaagtttaa agaaagtgtc
      181 cgcctctttg tcgtgaaacg cttgaagaat ttcttgatag taaaaatagg cgattcgtaa
      241 ttcgtcagag atcgaaagca gacgatccac cacttcactt tcggtgagat acgcccaacc
      301 aaaattgcgc catttggtcc atttgtcata aacaagcttg tcctgtcgtt tgatcagaag
      361 ccgagacaac gcctttaatt gtcgataata tttggcttgt gttggatcgt ttcgatcaaa
      421 gcttttcatg attcgtacgc gaatatcctc aaatttacga atgagatgtt tgacaatgtg
      481 aaaacgatcc acaacgagtt ttgcctttgg aaaacattcc tttatcagag aggcataaga
      541 aacattcatg tccgtcacga tgacctttac cttcaatcgt gcgcgtcgtt caaattgcat
      601 aaaataagaa acgagagcct ttttcttccg tgaaggaaga atatccagta attttccagt
      661 atcgccatcc atacaagaaa aggagtaggg accgacttga tttttcagtg aacgaaattc
      721 atccattaat aaacaacgcg gtaaccaatt tctttttact tttgtcatgg ggatcagaga
      781 agcgagtgtc cgagaaacgg tcgaccaaga aacgccgcaa tgcttagcaa ttgtcgtcat
      841 tgcttgtttt tcagttagct cttctaaaat tttatagcgc acttgtttag cgataaagca
      901 gtttctatcg acaatgtagg tattcgccgt aaaataggtg tggcagccac gacagtggta
      961 gcgttgtttc gctaaagcaa gataagtcag ttggttcgca catttattaa gttgaatgag
     1021 cgattttttc tttccgttct taacgatcat gccttcatga ttcatctgtt tacacaaagg
     1081 acagcgacga atttctttac ttaacgtcgc agaaacaaca agactcttga tattcttaat
     1141 gctcttcgat tccacatttg tgatgaaaat gtttttatct ttgattccaa gtagtagttt
     1201 gatatgatga agatcaggca tacagaacgc tcctttgatt gtgttgtggt gattcaattc
     1261 taacggagct tctgtatgtt ttgttttttc atgctcaaaa gggaccgata gaatgatttt
     1321 ctatcagtcc tagaaattat agagccgatt ttgaccgaca ggtataaccc tgccggtctt
     1381 tttttgctgc tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca
     1441 taataacaat cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa
     1501 tggaaaaaag caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg
     1561 tggaaaagcg ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt
     1621 tggggtggag actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc
     1681 atacaggcag cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca
     1741 aagaacaggg agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc
     1801 ccgcacagta cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga
     1861 tttctcccta tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg
     1921 tcgcatccgg ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg
     1981 aatataaggc gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg
     2041 ccgtgccggg aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa
     2101 ttcattcaac aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt
     2161 ttattcgtcg ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc
     2221 attaccgata tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg
     2281 aggaatattt aaaaccagaa aaatgagaaa aggatgttat gttatggaca gaaaaagatt
     2341 gacacagcgt ttcccgttcc tgcttccaat gagacgggcg cagagaaaaa tgtgctttta
     2401 tgcgggaatg agatttgacg gctgtcgcta tgcgcagacg ataggggaaa aatcgctttc
     2461 ccatttgctc tttgaaacgg attgcgcatt atacaaccac aataccggat ttgacatgat
     2521 ataccaggaa aacaaggtgt tcaacttaaa gctggcagca aagaccttaa acggcttatt
     2581 gataagaccg ggggaaacct tttctttctg gttgttcctg catacgccat tgacaattat
     2641 ccagcgcaga ggtcacgaag taaaggagtt tccagagcca aacagtgacg agattaaagg
     2701 ggtggatgca accatttcag agggctggat tgatttaaaa gtgcgaaacg ataccgactg
     2761 cacctaccaa atatgggtga ccttagatga tgagaaaatc atcggtcagg tgttcgccga
     2821 caaagagccc caagcattat acaaaattac aaatggcagt atccagtatg tccgtgaaag
     2881 tggcgggatt tatgaatatg cccaggttaa acggatgcaa gttgccttag gtaccgggga
     2941 aataatagat tgcaagctgc tttatacaaa caaatgcaaa atctgttatc ccctcccgga
     3001 aagtgtggat attcaggagg agaaccaatg agaaaaagtg tgggcattac tgtttttgga
     3061 tgcgaacagg atgaggcaaa tgctttccgc gctttatcgc cggattttca tattatccct
     3121 acgctgatta gcgatgcgat atcggcagac aacgcaaaat tggccgctgg caatcaatgc
     3181 gttagcgtaa gccataagtc cgaggtttcc gaggcgacaa ttcttgcgct gagaaaggtc
     3241 ggggtaaaat acatttctac ccgcagcatc ggctgcgatc acattgatac gactgccgcc
     3301 gagagaatgg gaatctcggt tggcacggtt gcgtattcgc cggacagcgt tgcggattat
     3361 gctttgatgc tgatgctgat ggccatacgg ggtgcaaaac ccaccatgca cgccgtggcg
     3421 caacaagatt tcagattgga tcgtatccgg gggaaagaac tgggggatat gactgtggga
     3481 gttattggaa ccggccatat cgggcaagcg gtcgtcaaaa ggctgcgggg atttggatgc
     3541 catgtgctgg cctatgataa cagccgaaaa atggatgcag attatgtcca gcttgatgag
     3601 cttctaaaaa acagcgatat tgttacgctc catgtgccgc tttgtgcgga tacccgccat
     3661 ctgatcggtc agaagcaaat tggagagatg aagcaaggcg catttttaat caacaccggg
     3721 cgcggagcgc ttgtcgatac cggggcgctg gtggaggcgt tggaaagcgg aaagctgggt
     3781 ggtgcggcac tggatgtgtt ggagggcgag gatcagtttg tttataccga ttgctcgcag
     3841 aaagtgcttg accatccctt tttgtcgcag cttttaagga tgccgaatgt gatcatcaca
     3901 ccccatacgg cgtactacac tgagcgtgtg ctgcaggata ctacagaaaa aacaatcagg
     3961 aattgtctca attttgaaag gagtttacag catgaataga ataaaagtcg caatcatctt
     4021 cggcggttgc tcggaggaac atgatgtgtc ggtaaaagcc gcaatagaaa ttgctgcgaa
     4081 cattgatacg gaaaaattcg atccgcacta catcggaatt acaaaaaacg gtgtatggaa
     4141 gctatgcaag aagccatgta cggaatggga agccgacagt ctccccgcca tactctcccc
     4201 ggataggaaa acgcatgggc tgcttgtcat gaaagaaagc gaatacgaaa cacggcgtat
     4261 tgatgtggct ttcccggttt tgcatggcaa atgcggggag gatggtgcga tacaggggct
     4321 gtttgtattg tctggtatcc cctatgtggg ctgtgatatt caaagctccg cagcttgcat
     4381 ggacaaatca ctggcctaca ttcttacaaa aaatgcgggc atcgccgttc ccgaatttca
     4441 aatgattgat aaaggtgaca agccggaggc gggtgcgctt acctaccctg tctttgtgaa
     4501 gccggcacgg tcaggttcgt cctttggcgt aaccaaagta aacggtacgg aagaacttaa
     4561 cgctgcgata gaagcggcag gacaatatga tggaaaaatc ttaattgagc aagcgatttc
     4621 gggctgtgag gtcgggtgtg cggtcatggg gaacgaggat gatttgattg tcggcgaagt
     4681 ggatcaaatc cggctgagcc acggtatctt ccgcatccat caggaaaacg agccggaaaa
     4741 aggctcagaa aatgcgatga ttacagttcc cgcagacatt ccggtcgagg aacgaaatcg
     4801 ggtgcaggaa acggcaaaga aagtatatcg ggtgcttgga tgcagagggc ttgcccgtgt
     4861 tgatcttttt ttgcaggagg atggcggcat cgttctaaat gaggtcaata ccctgcccgg
     4921 ttttacatcg tacagccgct acccacgtat gatggccgcc gcaggaatca cgcttcctgc
     4981 actgattgac agcctgatta cattggcgtt aaagaggtga taagtatgga aaatggtttt
     5041 ttgtttttag atgaaatgtt gcatggcgtc cgttgggatg ccaagtacgc cacatgggat
     5101 aacttcacag gaaaaccggt ggatgggtat gaggtgaatc gcatcatcgg cacaaaggcc
     5161 gtggcgtttg ctctgcgcga ggcacaaatc catgcggctg cgcttggcta tggcttgctt
     5221 ttatgggatg gatatcggcc aagaactgcg gtggactgct tcctgcgttg ggcagcgcaa
     5281 ccggaggaca atctcacaaa agaaaaattt taccccaata tagagcgagc cgagttgatt
     5341 acaaagggtt atgtggcctc acaatccagc catagccgtg gaagcgcaat tgatcttacg
     5401 ctctaccacc tggatacagg ggaacttgtt tcaatgggaa gtaacttcga ttttatggac
     5461 gaacggtcgc atcatacagc aaaagggata ggggatgcag aggcacaaaa tcgaagatgc
     5521 ttgcgtaaaa tcatggaaag cagcggattt cagtcttatc gctttgaatg gtggcactat
     5581 aagttgattg atgagccata ccccgatacc tattttaatt ttgccgtttc ataatgaaag
     5641 tattggtttt gtatcagatt ttggatttga taaaatctat gctggtcatg gacaatcttt
     5701 tactggaaag tggttaaagc atcttttgta atacattgca aattatagta ttcaatctgc
     5761 ccagcggcag aaaagaaaaa aaccgctgtt gggcagacct ttcccacgcc cattttgtat
     5821 tttgctggcg gcggttttga gaaatcaagg ccgccgttct tttctggcag caaaaggtgt
     5881 ggcggcccat aggaggatgc cgctatgctg ttttgctatt gccatgctct aatccacttc
     5941 caccactcaa aaaaagcctt gcccctccat gaactgaacc ccaaaagttg aactatttaa
     6001 tggactgt
//
LOCUS       DAYYGS010000268         5832 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.123, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000268 DAYYGS010000000
VERSION     DAYYGS010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.123"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            351..815
                     /locus_tag="ACHA44_003016"
     CDS             351..815
                     /locus_tag="ACHA44_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFM8906190.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            799..1428
                     /locus_tag="ACHA44_003017"
     CDS             799..1428
                     /locus_tag="ACHA44_003017"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFM8906191.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            1782..3260
                     /gene="msr(C)"
                     /locus_tag="ACHA44_003018"
     CDS             1782..3260
                     /gene="msr(C)"
                     /locus_tag="ACHA44_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HFM8906192.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            3307..3753
                     /locus_tag="ACHA44_003019"
     CDS             3307..3753
                     /locus_tag="ACHA44_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HFM8906193.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3952..4467)
                     /locus_tag="ACHA44_003020"
     CDS             complement(3952..4467)
                     /locus_tag="ACHA44_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HFM8906194.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            4584..5786
                     /locus_tag="ACHA44_003021"
     CDS             4584..5786
                     /locus_tag="ACHA44_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFM8906195.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         1805 a          972 c         1232 g         1823 t
ORIGIN      
        1 tgacaacatc ttgtcataac ttttttatta ctgtttaagc atactgatcc aatttctaat
       61 attcgacggg attcttgcaa aaggcgattt cggaaatttt tggcggtgta aaattatcta
      121 ctgcagaaga gctatcttct atagtttgat aagaatcaat aatcttggtt ttagcgttgt
      181 gctttcttta tcattttttc cgttcattct acaaaaaatt tcttttctct atacttttct
      241 attgagagcg ttttttttat tacacagctg attctttgac ttttttcttt ttttattcta
      301 ttattgacaa gtcaatcgtt gacgagtcaa ttattagaaa ggaggaaatt atgtctttag
      361 aagagattag tcacctttta tatcaaatca aattagccga tcaaaaaatc gtccagatct
      421 ttgaagaaaa tatcggtata agtcttacta gatacgagat catgatgaga ctgaaagaag
      481 aaaatccatt gctacaaagc agtttgcaag aaaagttgca gattgatcaa ggagcagtta
      541 ctcgtcatct gaaggtattg gaagcaaatg gctatgtttc gcgtcagaga aatcctcaga
      601 acaaccggga agtgtatgta gcgctaacag aaaaagccca gaagaaaatc aagcattgtg
      661 aaatggacca taaagataca ggttccgtgc tatccccggc tttcagcgat gtagaaatga
      721 aacaatttct aacactttta gataaattga atacccaact ttgcaatata acaacagaaa
      781 cgaaagaggt taataataat ggaacaaaaa aatgattttt caacaattat gactggtaga
      841 agatccgttc gcgtatatga tgaaacatac aaaatcccac acgaagaaat gctggaaatg
      901 atcaaagaag cgacgactgc accttcttct gtcaatatga aaccatggcg ctttgtggtc
      961 gctgaaagcg aggaagcaaa agaaatcttg cgtcctttga tccgtttcaa tacgagacaa
     1021 aatgatactt cttctgcaat ggtcatgatt tttggcgata tgcaatgtta cgaatatggc
     1081 gaagaaatct acaaccaagc ttacgaatca gggaagatgc caaaagaagt cagagatcaa
     1141 caattagctg cgatcattcc ttattacaag agtttttcaa gagaagaaat gaacgatgta
     1201 gtcaaagtcg attctagctt agcggctatg caattcatgt tagttgcgcg tgatcatggc
     1261 tatgaaacaa atccaattgg tggattcgaa gcagatcagt tggcagaggc atttggtttg
     1321 gataaagacc gttatgttcc agttattatt ttatcaatag gaaaagcggt agaagaaggt
     1381 tacgagtctg ttcgtttagc accagaaaag atcactatgt tcgaataaaa ggttttcagt
     1441 tactattcag tagctattgc tttttttgaa tcgtgtgtgg taacctaata aaaaaggagg
     1501 tcggaaaaat gactgcatcg atgaaattac gtttcgaact tttgaataac aactaaataa
     1561 attcaaaaga catgtataaa caacaatttc atggatggag tatgtccatt ttttcgaacg
     1621 acagtagaat aggtgaaagc tatgacagat ttggtgattt agtcatagtt atttcgttca
     1681 gagtaggaaa agatgtgatg cagttttttt gcaaacagat ttttctgctc ttttttagta
     1741 tcgataattg ttgttttaca tgaaatagga gtgtaaaaca tatggaaaat ttagcagtaa
     1801 atataacaaa tctacaagtc agctttggca accagctaga actatctatt gattctcttc
     1861 gtgtctatca gcaagatcgt atagggatca taggagaaaa tggtgtcggt aaatcgactt
     1921 tgctcaaact aatagccggt gaactttttc ccgatcatgg gaaaatccaa acagagatca
     1981 ccttcaacta cctgcctcaa ttaacctatc tcgctgaagc aaaggaccta aatttggaat
     2041 tagccagtca tttccagtta agactggaag aaacttcaga gcggaaatgg agcggagggg
     2101 aagaacgaaa gatcgagttg atacgtcttc tttcttctta tgaacaaggg atgcttctag
     2161 acgagccaac aacccatcta gatagaaaaa gtattgatcg actgattgaa gagcttcgtt
     2221 attattatgg tacgctggtt tttgttagtc atgatcgcta ttttctagat gagctagcat
     2281 cgaaaatctg ggaagtaaaa gacggagaaa tccgagagtt ttcggggaat tatagtgcct
     2341 atctcactca aaaggaattg gagaaaaaga ctcagctacg agaagcagag tcgatcatga
     2401 aagagaaaaa acgattggaa aaatcgatcc aagaaaagaa aaaacaagcg gaaaagttgg
     2461 aaaaagtgtc cagtaaaaag aaaaagcaac aaatcagact ggatcggttg tcttcctcta
     2521 aacaaaaaga cagtgtacaa aaagccatcc aaaagaatgc gaaaacatta gagagaagac
     2581 tccaaaaaat aggagaaaca accaagccgc agcagatgaa acaaatccgt tttccagtac
     2641 caaaatctct tgaactccac agccgctatc ccatcatggg acaaaatgtc caattggaaa
     2701 gaagtgggag aacattactg gtaaatggtg attttcagtt ttctttaggt aaaaaaatcg
     2761 cgattgtcgg tgaaaatggt tcaggtaaga caaccttatt agaacatatc cgcaaacaag
     2821 gagaaggaat ccttctctct ccgaaagtaa gctttcaagt atatcagcaa aagggttatc
     2881 aaatgacatc tgaagaatcc atcattcgtt ttgtcatgag acaaacagag ttttcagaat
     2941 cgcttgtccg tagtttgctg aatcacttag ggtttgctca ggaaactttg acgaaaccgt
     3001 tatgtacatt aagtggggga gaagcgaccc gtctgacgat tgctttgctt tttactaagc
     3061 caagtaatgt gttgctgtta gatgaaccga ctaattttat tgatatggca acgatcgaag
     3121 ctttagagaa gctaatgcaa atatatccgg gaacgatttt gtttacttct catgattctt
     3181 actttgtcga gcgtacggct gatgaagttt atgaaataaa agggcagaaa ataaaaaaag
     3241 tacttacgag aaatttttaa atgtgctacg atgattttcg tgacaaattt aaaggagtgg
     3301 tacagattgc ctacaaataa aaaagaagga ataattttta caacaatgat gtgttttttg
     3361 atggtcttgg ggatgagcgc gtataattta tggctgcatc aagatcttca tttatcaagt
     3421 ttgttgattg gtctagtgcc tggattcgtg gtggctttca ttttggatgt cttcatcatc
     3481 ggtgtgctgg cgaaaaaaat tgcctttaaa ctaccagtaa atccagaaag caaggtccaa
     3541 atgatcttaa ctatttcatg cttgatggtt ttaggaatgg tgacatgtat gtctttattc
     3601 ggtgtgatca tggaacaagg aatacctgat caattattct ctgcttatat gaatgcttgg
     3661 aaaatgaatg tgattgccgc tttaccgttg cagttgatca tcgttggccc gctttcaaga
     3721 agtgtgctaa aagcaattca aagtaatgaa tgatggaaca ctgttatttg aaaaaaggcc
     3781 ttggcatgct ttttgtcata gtctcttgaa tgcagttcta gaaaaatagt gatccgaacg
     3841 ataggttttg gcctgtcgtt cggatcgctt tttcttttcc aactgtattg ctcttttatt
     3901 gaagataatt tgctcttcaa tagatatttc tagaaaggtt ctgtttatcc cttactcaaa
     3961 aaactgctgc atttgttctc ttacttgctg acaccattgg agttcctgct gacaaagcat
     4021 aattctgcgc tggatcacaa cataacgtcc aaattcttgt ttcttgtcta gcgagaggtc
     4081 attcaatttt ttttgatttt tttgcatgat tttgttcagc tgttgttctc tagtgaatag
     4141 cagtccagta gctgttgttt tatcgagcgt cgaaaaagca tagatttttg ctagaaattc
     4201 atctttttgc gtaggagcgg gagtttcctc tttgatccat tcctctacaa gagcacgtcc
     4261 ttcatcagtc aagtggtaga tctttttttg agagtgctcg tctttgtcca aaacggtcac
     4321 ataaccctgc tggtctaact tggcaagcgt tgtatagatc tgactgtgat gggcttccca
     4381 aaaaattgtg atcagctgct tcaattcata cccagaacgt ggtttggata aaagcacgca
     4441 taataatata taggctaaag tattcataaa aatcctacct ctcgtcttat tttctttatt
     4501 atatcacagt aaagtttcca cgttttacat gtgcatattg acatgtaaaa taaatcatgt
     4561 atgatgctaa tagagagaaa gggatggaaa tattggagaa tcaaatagat atacaagaac
     4621 aaacaaatga agaaacaaag gggaaaagtt cagcgctagt tgtagcagct acctgtatga
     4681 tcgcttttat gggagtgggt ctagtagacc caatcttaaa aacgattgct gcacagttaa
     4741 acgctacgcc agctgaaacg accctgttat ttactagtta catgttagtc actggtgtgg
     4801 tgatgctatt tacaggtttc ttatctagtc ggatcggttt gaaaaaaaca ttgatgacag
     4861 gattgtttat catcgttgta ttcgctggtc ttggcggatt atctgggaat atcggaatgc
     4921 tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt atttgtctct actgctttag
     4981 caacgatcgt cagtatttta gttggtaaca cagaaaaagc aatcatgatg tatgaagcag
     5041 cacttggctg cggaatggct gttggccctc tagtcggtgg ggtcttgggc agtggttcat
     5101 ggcgttatcc attttttggg gtagcatttt tgatgttttg cggttttata gccctagcgg
     5161 tacttttacc tgaaactgag aaaccaaaga gaaaagtagg cctttgggaa ggtgtcaaag
     5221 cgttaagcaa tcataaactt cgttcagttg gactaatcgc attgttatat aatttcggat
     5281 tctttacttt acttgcgtat gcgccatttt tattgattgg ctattctgaa ttagaagtcg
     5341 gttttgtatt ttttggctgg ggtgtgcttc tggctattgc ttcgattttc gttgcaccgg
     5401 ctcttgagcg gaagtttacg acttatcgga caattttagc agcatttgtc ttattcatta
     5461 tttgtttgct gttattagga gctggaactt ctatgccagt ccttgtaccg atcagcattg
     5521 tgttagcagg atttttccaa ggaattatca atacactgtt gacgacgatt gctatggaaa
     5581 taccaggtct gcaacgaaac gttgcttcgt ccagctatag ttttgtccgc tttttcggtg
     5641 gagctttagc accatttatt gcaggtgtga ttggtgaaca tattggtgaa agctatgcgt
     5701 tttattttgc aggtgccatt gttcttatca gtgtatgttg tatcatttat caccgtaatt
     5761 attttgctac aaatgaagaa gaataagaaa tcagaggttg tgaaaaagct gtcctgcatc
     5821 aagtcataag aa
//
LOCUS       DAYYGS010000269         3910 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.149, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000269 DAYYGS010000000
VERSION     DAYYGS010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.149"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            109..801
                     /locus_tag="ACHA44_003022"
     CDS             109..801
                     /locus_tag="ACHA44_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFM8906196.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSWKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1128..1625
                     /gene="dfrG"
                     /locus_tag="ACHA44_003023"
     CDS             1128..1625
                     /gene="dfrG"
                     /locus_tag="ACHA44_003023"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HFM8906197.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1697..3649)
                     /locus_tag="ACHA44_003024"
     CDS             complement(1697..3649)
                     /locus_tag="ACHA44_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HFM8906198.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3656..>3910)
                     /locus_tag="ACHA44_003025"
     CDS             complement(3656..>3910)
                     /locus_tag="ACHA44_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906199.1"
                     /translation="KGVHFPMQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTES
                     EAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1315 a          661 c          665 g         1269 t
ORIGIN      
        1 tgatagtatt tatattaaaa ttacatatgt aatttaaaaa cgcttgacat tgtgtttgag
       61 atcacttact tttagcatgt aaaagtattg gattaaggag ctatgcaaat gaaaaagaaa
      121 tttattactg gtgggattgt cgttgcattt ctatgtctaa ttggctacac aggcacgcgg
      181 tacgcaaatg aaattatttt gtggggtgga gagacagata ttgatacaat caatgagaat
      241 ctgacacagc ttgatcatac attgacagat aaagagcaag cgattacaga tcttaccgct
      301 cgactagcac aaaaagaaca gacattgcaa gattcaaagg cagatgtcca agaataccaa
      361 acaaaagtaa atgaattaga aaaccaaaaa aatcaatcgg cagcagaaaa acaaaactta
      421 gaaagtcaat tagtcagcaa ggatgcacaa ctacaaagca aacaaaatga aatcaatgtc
      481 aaactagatg agatcaacca aaaaaatgat gaaatcaatc atctgaatgc agattggaca
      541 gcaagactac aagaagcaca agcaaaactt tcagaattac aaaatactgt aaatggtttg
      601 aatcaacaaa tcgcaacact gacacaagaa aaaacaaatg tagaaactca gttaacagat
      661 acacaaaaga aattagacga agcaactgct gaaaactcaa gctggaaaca atatattcaa
      721 aagttagaaa aagcaaaaga tgaagtggaa gatacagctg ataaatctca gcaaattgtt
      781 gaagaacatt tgcgtaaata atctttctta acaagtaaac ctaaattagt aaagaagcaa
      841 cagtaatcta tactaagtag ttcagttttg gagtacaaaa tgacaaaatg tacttcaaaa
      901 tggactactt ttttcatttt aacttacata ccaaattaca tacaaaatga tttataaaat
      961 gcaattgaaa atgataattg aatgaaaatt tgtgattttg tggtattatg ataatataac
     1021 ttatagaaaa aattgcttat tcaactaacg ggcaggataa tgagaaaagg ttttttgata
     1081 gaaataagaa attagatggt aaattagtta aaaaggctgt gttataaatg aaagtttctt
     1141 tgattgctgc gatggataag aatagagtga ttggcaaaga gaatgacatt ccttggagga
     1201 ttcccaagga ctgggaatat gttaaaaata ctacaaaggg acatccgata atattaggta
     1261 ggaagaacct tgaatcaatc ggaagagcct tacctgacag aagaaatatt attctgacga
     1321 gagataaggg gtttaccttt aatggttgtg aaattgttca ttcaatagaa gatgtttttg
     1381 agttatgtaa aaacgaagaa gaaattttta ttttcggagg agaacagatt tataatttgt
     1441 ttttccctta tgttgagaaa atgtacatca caaaaataca tcatgaattc gaaggagata
     1501 ctttttttcc agaagtgaat tatgaggaat ggaatgaggt atttgcccaa aaagggataa
     1561 agaatgataa aaatccgtat aactactatt ttcatgtata tgaaagaaaa aacttattga
     1621 gttaacgggt gcgttaattg aagaagaaca gtaaagataa agcacaagag cttgggatta
     1681 ctccaagctc tttttgtcat ttgaaataaa ttatatcttt ccaatcaaat tgtttatctg
     1741 taatccctaa tctttgagca ggtgtcaaaa cctttttatc cgctgattta aaagggcgac
     1801 aaaagttgta gtaagttctt agaattgtaa tcgcaaattg agcatacttc ggattgaagt
     1861 tagcgtaaat atagctcttc ttatcaccac gagctgttgt taatggtctt tcaagagatg
     1921 atatataacg tcttatctgt tgaataaagc tattggtcga atggtcatta acatttaata
     1981 gcatctttgc gatttcgttt ggttccagag cagataaatc cgttctacaa tcaactgaat
     2041 aaaatccttt atccttagtg gctaatggat gtttgatagg gttatcggca tatttgcgat
     2101 atctttctcc atctttacta atagcctctt cgtggaattg atgacttgtt agtaattcag
     2161 ttagataatg agatgccaaa gtccgtagaa actttgtttt gaatcctctg atatctcccc
     2221 aatcgagcag ttctgctttt gcttgtccga attcttttaa gcattgtttt ctgcttttcg
     2281 tcttgttaac ttggcaaata aaatgatgtg catcgctcaa ccttaattct ttcgtgaata
     2341 ctctataaaa ggcgtttcta atagagaagt catcatcact aatcattcgc cattcgtcgg
     2401 cattaaccat ttgtttaatt aaccaatagt gagcagttgt agtataagga gcatcaacat
     2461 ttaaaccatc gacaaatcta ctcctattag taattttccc taattcgtga agataagcat
     2521 tcctattttc gctatcgtta gcggatggtt cttgtggata ataagaccaa tctaacctat
     2581 catttttacg actaaaagta ttgagatggt cttctttgaa ttttcttgta tcttcattta
     2641 attcatccat tgaaatattc caatcatagg caacatcaga acggaacaca tatcttgata
     2701 agacctcagc agatacaact atatacgttt gcatattaag gtcttcaaac cccgtatatt
     2761 tctttgaacc ttgtcctttc ttacggacgt tatttaggta gtagtgcatc ttgtccgtat
     2821 taagccacat ctcgttaaat ttctttgttt gaaggggttg tgtctcatat cgttctaaaa
     2881 actccaaaca acgcctatac agccactcca gcttatgata gtaggttcca actccaattc
     2941 ctaaaatatc acaagtacga ctgactgaaa cccttccaac aaccatctta gcgaacattg
     3001 gaagaatggt gttcttttgt tggtgataag tagtggtttc ttctcttttt ggtagaacat
     3061 tagtgaactt cttacacgcc ttacattgat agcgttgaga tttgccccta cttttccccc
     3121 ttttgtaaaa ttgcttcggc tcattgaatg gggttgattc ttcttccgag cagccttctt
     3181 tatgaaattg ataatctggc tcaacatctt ggatgctatt gatttctata aggcgtttaa
     3241 tttcttcaac aacagaccaa ttagataaag gggttactgt acagttttgc gatacacctc
     3301 tgccaatagg attgtcattg cagtacatac ctttatgacc tttatctttg gaactaccag
     3361 tcattttata tcgacttggt ttccctttta catctttgaa tttgtgctgt tctttcccaa
     3421 aattacaaca aaacggattg atgcagtaat tatattgaat gggatattca gaaccgttcc
     3481 atttaaaaga cgttggcaag aacaacattt tgttatacct tgttgcaaat cgttttggag
     3541 ttaataaatc atattgttta gccctatctt ttaattcttc gtttgaaatc ggaattttaa
     3601 catctacaat ttcatcttcc ttcgtggcta atcttttaag cttggtcatt gtctatcaac
     3661 ccactctatc tttgataccc attttttcaa caatccgctt attacttcgc ttgttggcaa
     3721 tccattcaat aaacttctta tcgtctaaaa tgttcaataa gaattcatcc actgcttcgc
     3781 tttcagtacg ctctgcatat tcagcgtatt gcttaactat cacccttact tgctctgata
     3841 tttcccaatc cacagactta gcgttcttat tttttggctc aagaaattgc ataggaaaat
     3901 gcaccccttt
//
LOCUS       DAYYGS010000270         3795 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.153, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000270 DAYYGS010000000
VERSION     DAYYGS010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.153"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            110..193
                     /locus_tag="ACHA44_003026"
     CDS             110..193
                     /locus_tag="ACHA44_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFM8906200.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA44_003027"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="ACHA44_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFM8906201.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="ACHA44_003028"
     CDS             complement(1318..2181)
                     /locus_tag="ACHA44_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFM8906202.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="ACHA44_003029"
     CDS             complement(2183..2455)
                     /locus_tag="ACHA44_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFM8906203.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="ACHA44_003030"
     CDS             complement(2472..2681)
                     /locus_tag="ACHA44_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HFM8906204.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(2759..3655)
                     /locus_tag="ACHA44_003031"
     CDS             complement(2759..3655)
                     /locus_tag="ACHA44_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFM8906205.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
BASE COUNT         1174 a          662 c          586 g         1373 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattct
     2761 attctttttc attttctaat tgaatgattc gctcaatcat ttcaaaaaat acgttctcat
     2821 acatagacaa aacttttttg tcatagcctt tgtgttctgt aatgccattt ttagaccaag
     2881 tacttacttt attacttcgc ttgataatat ttcggaaaac caaattatcc tctttatgtt
     2941 ctttgtacag ttcttccagg tttgatttta tcgttgcgct gtccgtatca actaaataag
     3001 gaacaaaacc aatcatatct agtccagggt taaactgttc ttgcaaatca atcaaatagg
     3061 aaatatagtt ttgaatgttg tttgtacttt cttcttctgc ttgtaaaggg atcataacgt
     3121 aatcactcgc cacgattgca ttatttgtat aaacgcttgg cgttggtaca gtatcaataa
     3181 taataagatc atagtcactt tttaaaggtg ctaaaagagt agcaagcaat ctactttcat
     3241 tttcaaacgt ccatgagcga gttaattttg gcagtaacat caaatcaaac gtgccaggga
     3301 tcaagtctaa attatcagtc aaatgaataa tagaagaagc caagtttcca tttttcaagc
     3361 cttcataaaa attgacacgt ggcaattcta cctcaaaagt ttttgctaag tcttttgtca
     3421 atgttgcctg taagtcctta tcgatcatta aaacttttaa attcaatttg tctgtcaagt
     3481 aagcaaacat agtcgataat ttggactttc caacaccacc tttaaagtaa ttattcaaga
     3541 taataattgc ttcattttta ttgtttaaaa tacgtcttaa ttcttcaagt atttttagtt
     3601 tttccttctc cataactacg ccccattctt tttttgtata gtaatattgt atcattaaga
     3661 atcacaaatc acaagtgatt tgtgattaat cacttgtgat ttgtgataag tgatttgtga
     3721 ttaatatata aaagccctct ttaaagggct tttataggtt ctgttgcaaa gttttaaata
     3781 aagaataaaa tccct
//
LOCUS       DAYYGS010000271         3391 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.164, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000271 DAYYGS010000000
VERSION     DAYYGS010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.164"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            29..214
                     /locus_tag="ACHA44_003032"
     CDS             29..214
                     /locus_tag="ACHA44_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFM8906206.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            372..539
                     /locus_tag="ACHA44_003033"
     CDS             372..539
                     /locus_tag="ACHA44_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906207.1"
                     /translation="MKKRNFIMTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVS
                     RHWITAGLMRL"
     gene            1157..1816
                     /gene="vanR-B"
                     /locus_tag="ACHA44_003034"
     CDS             1157..1816
                     /gene="vanR-B"
                     /locus_tag="ACHA44_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance response regulator
                     transcription factor VanR-B"
                     /protein_id="HFM8906208.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFEGDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            1819..3180
                     /gene="vanS-B"
                     /locus_tag="ACHA44_003035"
     CDS             1819..3180
                     /gene="vanS-B"
                     /locus_tag="ACHA44_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin resistance histidine kinase VanS-B"
                     /protein_id="HFM8906209.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPNFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRSRKSGRSGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLT
                     STL"
     gene            complement(3271..>3391)
                     /locus_tag="ACHA44_003036"
     CDS             complement(3271..>3391)
                     /locus_tag="ACHA44_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFM8906210.1"
                     /translation="LHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1011 a          649 c          855 g          876 t
ORIGIN      
        1 attataatgt accctgtgga ggtgtactat ggactacatg acgttgaaag aggcaggcga
       61 gaaatggggt gtgcccccac gatggataaa ctactattgt gctgcgggac gcatccccgg
      121 cgctgtaaag atggcgacga tctggctcat tcccaaaact gcggaaaagc cgattgacag
      181 gcgtacaaag aaaggaaagg cggttactga atagtataat gaaaccggcg cattttggat
      241 atggttttga agagagagaa agcatggtat tttgtcacgg aaaaactata agatgataga
      301 tggcaggctg ctccagaccg ataaaaagta ttctcatctg aaacagaaag aaaagattta
      361 cacatggatg tatgaagaag cgaaacttta tcatgacaga aatggaaaat gcccgaaaca
      421 aagacgagga agctgctgct gtatcagccg tatatggtag aattgaaaag gccggcatct
      481 ggattcttta tggtgaagtc tccagacatt ggataactgc tggtctaatg cgcctttgag
      541 tatggggtga ttgattttaa taccggggag gtacggcttt ttgaggaaaa tattctggga
      601 tttacatttg gcgattatta catttcctgt aactgcaagg acacggatga tgaagatatg
      661 aggaatatag acttgctgat tttctactgt cttgaacggt attaggactg aatctgctgc
      721 gcggagtgcg gcaagagcga ccggagggag tacacacagg ccctgcctgt gtgaaaagcc
      781 ataccgcaag gtgcattcgc aaaaaagagg ccgcgcattt tgctgaggct tggggtgtcc
      841 ccaacaagca aaacgagaac gccgcaggcg ggcaagtggt ggatcagaat atccgtaaag
      901 gatattctga tccattgctt gccagtatta tcgggatgtt ttgacaaccg ctgtaatcga
      961 aacatcccaa acattaagaa ggtgttaatt attattatct tgatttaatt cgtttaaacc
     1021 aataatatag atacatacac catgaaaaga agcgagtatc ccaatgacgg atgattaaaa
     1081 tccgaaaaga aatcgctttg aaactacagg gaaactacag actgttatgt tatcttctta
     1141 aaatggaggg atttttatgt cgatacgaat tctacttgtc gaggatgatg atcatatctg
     1201 taatacagta agggcgtttc tggctgaggc aggatatcag gtggatgcct gcacagatgg
     1261 aaatgaggca tacaccaagt tttacgaaaa cacttatcaa ctggttattc ttgatattat
     1321 gctgcccggt atgaacgggc atgaactttt gcgtgaattt cgtgcgaaaa atgatactcc
     1381 cattctgatg atgacagccc tgtcggatga cgaaaaccaa atccgggcgt ttgatgcaga
     1441 ggcagacgac tatgtaacaa agccattcaa gatgcagatt ttactaaagc gggtggaagc
     1501 cctgctgcgg cgcagcggtg cgctggcaaa ggaaatccgt gtcggcaggc tgacacttct
     1561 gccggaggat ttcacggtac tttgtgacgg tacagagctg cccctgacac gaaaagaatt
     1621 tgaaatcctt ttgctgctgg tgcagaacaa aggcagaacc ttaacgcatg aaatcatttt
     1681 gtcacgtata tggggatatg actttgaggg tgatggcagc acagtccaca ctcatatcaa
     1741 aaatttgcgg gccaagctgc cggaaaatat catcaaaacc attcgcggtg taggataccg
     1801 attggaggaa tcataataat ggaaagaaaa gggattttca ttaaggtttt ttcctatacg
     1861 atcattgtcc tgctactgct tgtcggtgta acagcaacac tgtttgcaca gcaatttgtg
     1921 tcttatttca gagtgatgga attacagcaa acagtaaaat cctatcagcc attggtggaa
     1981 ctgattcaga atagcgatag gcttgatatt caagaggtgg cagggctgtt tcactacaat
     2041 aaccaatcct ttgagtttta tattgaagat aaagagggaa gcgtactcta tgccacacca
     2101 aatgccaata catcaaatag ttttagaccc aactttcttt atgtggtaca tagagatgat
     2161 aatatttcga ttgttgctca aagcaaggca ggtgtgggat tgctttatca agggctgaca
     2221 attcggggaa ttgttatgat tgcgataatg gttgtattca gccttttatg cgcgtatatc
     2281 tttgcgcggc aaatgacaac gccgatcaaa gccttagcgg acagtgcgaa taaaatggca
     2341 aacctgaaag atgtaccgcc gccgctggag cgaaaggatg agcttggcgc actggctcat
     2401 gatatgcatt ccatgtatgt caggctgaaa gaaaccatcg caaggctgga ggatgaaatc
     2461 gcaagggaac atgagttgga ggaaacacag cgatatttct ttgcggcagc ttctcatgag
     2521 ctaaaaacgc ccatcgcggc tacaagcgtt ctgttggagg gaatgcttga aaatatcggt
     2581 gactacaaag atcattctaa gtatctgcgc gaatgcatca aaatgatgga taggcagggc
     2641 aaaatcattt ccgaaatact ggagcttgtc agcctgaatg atgggagaat cgtacccata
     2701 gctgaaccgt tggacatagg gcgcacggtt gccgagttgc tgcccgattt tcaaaccttg
     2761 gcagaggcaa acaaccagcg gttcgtcaca gatattccag ccgggcaaat tgtcctgtcc
     2821 gatccgaggc tgctccaaaa ggcactatcc aatgtcatat tgaatgcagt tcagaacacg
     2881 ccgcagggag gcgaggtacg gatatggagt gagcctggtg ctgaaaaatg ccgccttttt
     2941 gttttgaaca tgggcgttca cattgatgat actgcgcttc caaggctgtt caccccattc
     3001 tatcgcattg atcaggcgcg aagcagaaaa agtgggcgaa gcagaaaaag tgggcgaagc
     3061 ggtttaggac ttgccatcgt acaaaaaacg ctggatgcca tgagcctcca gtatgcgctg
     3121 gaaaacacct cggatggcgt tttgttctgg ctggatttac cgctcacatc aacattgtaa
     3181 atatttaaaa cttaaatgat tttgggctct ttgtcaaata ggactgatag agtgagataa
     3241 gaacatctaa ggtagaattc cttttctact ttagatgttt tttgattcat aggtaatcag
     3301 attatttagt aagaaaattc tggtcttcat tttccgaaag cttcgaaagc cataagcgac
     3361 tcgtttcaat gctttgatgt gggtatgcag a
//
LOCUS       DAYYGS010000272         1401 bp    DNA     linear   BCT 19-OCT-2024
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE8259
            SAMEA115709638-rid22597373.guided.216, whole genome shotgun
            sequence.
ACCESSION   DAYYGS010000272 DAYYGS010000000
VERSION     DAYYGS010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA115709638
            Sequence Read Archive: ERR13199356
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002290501.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002290501.1
            Genome Coverage       :: 135x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/18/2024 16:24:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 3,038
            CDSs (total)                      :: 2,977
            Genes (coding)                    :: 2,881
            CDSs (with protein)               :: 2,881
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 96
            CDSs (without protein)            :: 96
            Pseudo Genes (ambiguous residues) :: 0 of 96
            Pseudo Genes (frameshifted)       :: 40 of 96
            Pseudo Genes (incomplete)         :: 44 of 96
            Pseudo Genes (internal stop)      :: 36 of 96
            Pseudo Genes (multiple problems)  :: 18 of 96
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA115709638-rid22597373.guided.216"
                     /isolate="VRE8259"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Denmark"
     gene            184..533
                     /locus_tag="ACHA44_003037"
                     /pseudo
     CDS             184..533
                     /locus_tag="ACHA44_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741963.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(805..1095)
                     /locus_tag="ACHA44_003038"
     CDS             complement(805..1095)
                     /locus_tag="ACHA44_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFM8906211.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          446 a          262 c          203 g          490 t
ORIGIN      
        1 acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc agttctttgt
       61 tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata tttcctaata
      121 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
      181 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      241 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
      301 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
      361 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
      421 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
      481 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
      541 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
      601 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
      661 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
      721 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
      781 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
      841 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
      901 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
      961 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
     1021 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
     1081 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1141 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1201 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagattt
     1261 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
     1321 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
     1381 caaaatgtaa gttaaaatgt a
//
